LOCUS       DAJTRM010000001       136304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRM010000001 DAJTRM010000000
VERSION     DAJTRM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.001"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1199
                     /gene="cscA"
                     /locus_tag="OO322_000001"
     CDS             <1..1199
                     /gene="cscA"
                     /locus_tag="OO322_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5905039.1"
                     /translation="QHEPIALALGDDNDKDGCFSGSAVDDNGVLSLIYTGHVWLDGAG
                     NDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWREADTWWMVVGAKDPG
                     NTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPDFFSLGDQHYLMFSPQGMNAEG
                     YSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFLAKDGRRIVIGWMDM
                     WESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQHQSVSPRRISNKYVL
                     QENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSERLILWRYYPHENLDGYRS
                     IPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELSLYASHGVAVLQHG
                     ALWLLG"
     gene            complement(1207..2265)
                     /locus_tag="OO322_000002"
     CDS             complement(1207..2265)
                     /locus_tag="OO322_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5905040.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2433..2651
                     /locus_tag="OO322_000003"
                     /pseudo
     CDS             2433..2651
                     /locus_tag="OO322_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2669..3997
                     /gene="dsdA"
                     /locus_tag="OO322_000004"
     CDS             2669..3997
                     /gene="dsdA"
                     /locus_tag="OO322_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5905041.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4105..5643)
                     /gene="emrY"
                     /locus_tag="OO322_000005"
     CDS             complement(4105..5643)
                     /gene="emrY"
                     /locus_tag="OO322_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5905042.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5643..6806)
                     /gene="emrK"
                     /locus_tag="OO322_000006"
     CDS             complement(5643..6806)
                     /gene="emrK"
                     /locus_tag="OO322_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5905043.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7222..7836
                     /gene="evgA"
                     /locus_tag="OO322_000007"
     CDS             7222..7836
                     /gene="evgA"
                     /locus_tag="OO322_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5905044.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7841..11434
                     /gene="evgS"
                     /locus_tag="OO322_000008"
     CDS             7841..11434
                     /gene="evgS"
                     /locus_tag="OO322_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5905045.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11490..12635)
                     /gene="yfdE"
                     /locus_tag="OO322_000009"
     CDS             complement(11490..12635)
                     /gene="yfdE"
                     /locus_tag="OO322_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5905046.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12709..13653)
                     /gene="yfdV"
                     /locus_tag="OO322_000010"
     CDS             complement(12709..13653)
                     /gene="yfdV"
                     /locus_tag="OO322_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5905047.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13723..15417)
                     /gene="oxc"
                     /locus_tag="OO322_000011"
     CDS             complement(13723..15417)
                     /gene="oxc"
                     /locus_tag="OO322_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5905048.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15471..16721)
                     /gene="frc"
                     /locus_tag="OO322_000012"
     CDS             complement(15471..16721)
                     /gene="frc"
                     /locus_tag="OO322_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5905049.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17234..17857)
                     /locus_tag="OO322_000013"
     CDS             complement(17234..17857)
                     /locus_tag="OO322_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5905050.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18154..18429
                     /locus_tag="OO322_000014"
     CDS             18154..18429
                     /locus_tag="OO322_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905051.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18506..18748)
                     /locus_tag="OO322_000015"
     CDS             complement(18506..18748)
                     /locus_tag="OO322_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5905052.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19101..20021
                     /gene="lpxP"
                     /locus_tag="OO322_000016"
     CDS             19101..20021
                     /gene="lpxP"
                     /locus_tag="OO322_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5905053.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20376..20447
                     /gene="ypdK"
                     /locus_tag="OO322_000017"
     CDS             20376..20447
                     /gene="ypdK"
                     /locus_tag="OO322_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5905054.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20512..21750)
                     /gene="alaC"
                     /locus_tag="OO322_000018"
     CDS             complement(20512..21750)
                     /gene="alaC"
                     /locus_tag="OO322_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5905055.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22127..23613
                     /locus_tag="OO322_000019"
                     /pseudo
     CDS             22127..23613
                     /locus_tag="OO322_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23628..24362
                     /gene="ypdB"
                     /locus_tag="OO322_000020"
     CDS             23628..24362
                     /gene="ypdB"
                     /locus_tag="OO322_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5905056.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24375..25232
                     /locus_tag="OO322_000021"
                     /pseudo
     CDS             24375..25232
                     /locus_tag="OO322_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25235..27730)
                     /gene="ptsP"
                     /locus_tag="OO322_000022"
     CDS             complement(25235..27730)
                     /gene="ptsP"
                     /locus_tag="OO322_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5905057.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(27755..28792)
                     /gene="ypdE"
                     /locus_tag="OO322_000023"
     CDS             complement(27755..28792)
                     /gene="ypdE"
                     /locus_tag="OO322_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5905058.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28792..29875)
                     /gene="ypdF"
                     /locus_tag="OO322_000024"
                     /pseudo
     CDS             complement(28792..29875)
                     /gene="ypdF"
                     /locus_tag="OO322_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(29890..31137)
                     /locus_tag="OO322_000025"
     CDS             complement(29890..31137)
                     /locus_tag="OO322_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5905059.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31159..31485)
                     /locus_tag="OO322_000026"
     CDS             complement(31159..31485)
                     /locus_tag="OO322_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5905060.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31704..32669)
                     /gene="glk"
                     /locus_tag="OO322_000027"
     CDS             complement(31704..32669)
                     /gene="glk"
                     /locus_tag="OO322_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5905061.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            32873..34129
                     /locus_tag="OO322_000028"
     CDS             32873..34129
                     /locus_tag="OO322_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5905062.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34244..34570
                     /locus_tag="OO322_000029"
     CDS             34244..34570
                     /locus_tag="OO322_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5905063.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34711..35949)
                     /locus_tag="OO322_000030"
     CDS             complement(34711..35949)
                     /locus_tag="OO322_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5905064.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36285..37487
                     /gene="nupC"
                     /locus_tag="OO322_000031"
     CDS             36285..37487
                     /gene="nupC"
                     /locus_tag="OO322_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5905065.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37537..39649)
                     /locus_tag="OO322_000032"
                     /pseudo
     CDS             complement(37537..39649)
                     /locus_tag="OO322_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(39857..39932)
                     /locus_tag="OO322_000033"
     tRNA            complement(39857..39932)
                     /locus_tag="OO322_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39897..39899),aa:Ala,seq:ggc)
     gene            complement(39972..40047)
                     /locus_tag="OO322_000034"
     tRNA            complement(39972..40047)
                     /locus_tag="OO322_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40012..40014),aa:Ala,seq:ggc)
     gene            40268..40627
                     /locus_tag="OO322_000035"
     CDS             40268..40627
                     /locus_tag="OO322_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5905066.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40629..41021
                     /locus_tag="OO322_000036"
     CDS             40629..41021
                     /locus_tag="OO322_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5905067.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41073..42488)
                     /gene="gltX"
                     /locus_tag="OO322_000037"
     CDS             complement(41073..42488)
                     /gene="gltX"
                     /locus_tag="OO322_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5905068.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            42747..42822
                     /locus_tag="OO322_000038"
     tRNA            42747..42822
                     /locus_tag="OO322_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42780..42782,aa:Val,seq:tac)
     gene            42867..42942
                     /locus_tag="OO322_000039"
     tRNA            42867..42942
                     /locus_tag="OO322_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42900..42902,aa:Val,seq:tac)
     gene            42947..43022
                     /locus_tag="OO322_000040"
     tRNA            42947..43022
                     /locus_tag="OO322_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42980..42982,aa:Lys,seq:ttt)
     gene            43053..43178
                     /locus_tag="OO322_000041"
     ncRNA           43053..43178
                     /ncRNA_class="other"
                     /locus_tag="OO322_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43298..43639
                     /locus_tag="OO322_000042"
     CDS             43298..43639
                     /locus_tag="OO322_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5905069.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(43630..44556)
                     /locus_tag="OO322_000043"
     CDS             complement(43630..44556)
                     /locus_tag="OO322_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5905070.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            44646..45644
                     /locus_tag="OO322_000044"
     CDS             44646..45644
                     /locus_tag="OO322_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5905071.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(45641..45859)
                     /locus_tag="OO322_000045"
     CDS             complement(45641..45859)
                     /locus_tag="OO322_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5905072.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(45861..47876)
                     /gene="ligA"
                     /locus_tag="OO322_000046"
     CDS             complement(45861..47876)
                     /gene="ligA"
                     /locus_tag="OO322_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5905073.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(47947..48933)
                     /gene="zipA"
                     /locus_tag="OO322_000047"
     CDS             complement(47947..48933)
                     /gene="zipA"
                     /locus_tag="OO322_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5905074.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49163..49924
                     /gene="cysZ"
                     /locus_tag="OO322_000048"
     CDS             49163..49924
                     /gene="cysZ"
                     /locus_tag="OO322_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5905075.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50109..51080
                     /gene="cysK"
                     /locus_tag="OO322_000049"
     CDS             50109..51080
                     /gene="cysK"
                     /locus_tag="OO322_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5905076.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51464..51721
                     /gene="ptsH"
                     /locus_tag="OO322_000050"
     CDS             51464..51721
                     /gene="ptsH"
                     /locus_tag="OO322_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5905077.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            51766..53493
                     /gene="ptsI"
                     /locus_tag="OO322_000051"
     CDS             51766..53493
                     /gene="ptsI"
                     /locus_tag="OO322_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5905078.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53534..54043
                     /gene="crr"
                     /locus_tag="OO322_000052"
     CDS             53534..54043
                     /gene="crr"
                     /locus_tag="OO322_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5905079.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54085..54936)
                     /gene="pdxK"
                     /locus_tag="OO322_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54085..54936)
                     /gene="pdxK"
                     /locus_tag="OO322_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5905080.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55041..55415
                     /locus_tag="OO322_000054"
     CDS             55041..55415
                     /locus_tag="OO322_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5905081.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55448..56171
                     /locus_tag="OO322_000055"
                     /pseudo
     CDS             55448..56171
                     /locus_tag="OO322_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56375..57286)
                     /gene="cysM"
                     /locus_tag="OO322_000056"
     CDS             complement(56375..57286)
                     /gene="cysM"
                     /locus_tag="OO322_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5905082.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57420..58517)
                     /gene="cysA"
                     /locus_tag="OO322_000057"
     CDS             complement(57420..58517)
                     /gene="cysA"
                     /locus_tag="OO322_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5905083.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58507..59382)
                     /gene="cysW"
                     /locus_tag="OO322_000058"
     CDS             complement(58507..59382)
                     /gene="cysW"
                     /locus_tag="OO322_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5905084.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59382..60215)
                     /gene="cysT"
                     /locus_tag="OO322_000059"
     CDS             complement(59382..60215)
                     /gene="cysT"
                     /locus_tag="OO322_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5905085.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60215..61231)
                     /gene="cysP"
                     /locus_tag="OO322_000060"
     CDS             complement(60215..61231)
                     /gene="cysP"
                     /locus_tag="OO322_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5905086.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61535..62326)
                     /gene="ucpA"
                     /locus_tag="OO322_000061"
     CDS             complement(61535..62326)
                     /gene="ucpA"
                     /locus_tag="OO322_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5905087.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62513..63313)
                     /gene="murR"
                     /locus_tag="OO322_000062"
     CDS             complement(62513..63313)
                     /gene="murR"
                     /locus_tag="OO322_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5905088.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63477..64373
                     /gene="murQ"
                     /locus_tag="OO322_000063"
     CDS             63477..64373
                     /gene="murQ"
                     /locus_tag="OO322_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5905089.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64377..65801
                     /gene="murP"
                     /locus_tag="OO322_000064"
     CDS             64377..65801
                     /gene="murP"
                     /locus_tag="OO322_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5905090.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(65981..66880)
                     /gene="yfeX"
                     /locus_tag="OO322_000065"
     CDS             complement(65981..66880)
                     /gene="yfeX"
                     /locus_tag="OO322_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5905091.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(66976..67551)
                     /locus_tag="OO322_000066"
     CDS             complement(66976..67551)
                     /locus_tag="OO322_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5905092.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67612..68061)
                     /locus_tag="OO322_000067"
     CDS             complement(67612..68061)
                     /locus_tag="OO322_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5905093.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68048..68473)
                     /locus_tag="OO322_000068"
     CDS             complement(68048..68473)
                     /locus_tag="OO322_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5905094.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            68687..69556
                     /gene="amiA"
                     /locus_tag="OO322_000069"
     CDS             68687..69556
                     /gene="amiA"
                     /locus_tag="OO322_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5905095.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69560..70459
                     /gene="hemF"
                     /locus_tag="OO322_000070"
     CDS             69560..70459
                     /gene="hemF"
                     /locus_tag="OO322_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5905096.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70465..71517)
                     /gene="eutR"
                     /locus_tag="OO322_000071"
                     /pseudo
     CDS             complement(70465..71517)
                     /gene="eutR"
                     /locus_tag="OO322_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71563..72063)
                     /gene="eutK"
                     /locus_tag="OO322_000072"
     CDS             complement(71563..72063)
                     /gene="eutK"
                     /locus_tag="OO322_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5905097.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72076..72735)
                     /gene="eutL"
                     /locus_tag="OO322_000073"
     CDS             complement(72076..72735)
                     /gene="eutL"
                     /locus_tag="OO322_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5905098.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            73917..74708
                     /locus_tag="OO322_000074"
     CDS             73917..74708
                     /locus_tag="OO322_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905099.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75428..75823
                     /locus_tag="OO322_000075"
     CDS             75428..75823
                     /locus_tag="OO322_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5905100.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            75841..76104
                     /locus_tag="OO322_000076"
     CDS             75841..76104
                     /locus_tag="OO322_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905101.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76127..76669
                     /locus_tag="OO322_000077"
     CDS             76127..76669
                     /locus_tag="OO322_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905102.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            76808..77095
                     /locus_tag="OO322_000078"
     CDS             76808..77095
                     /locus_tag="OO322_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905103.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77134..77340
                     /locus_tag="OO322_000079"
     CDS             77134..77340
                     /locus_tag="OO322_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905104.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            77652..78137
                     /locus_tag="OO322_000080"
     CDS             77652..78137
                     /locus_tag="OO322_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5905105.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78118..78396
                     /locus_tag="OO322_000081"
     CDS             78118..78396
                     /locus_tag="OO322_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905106.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78389..79849
                     /locus_tag="OO322_000082"
     CDS             78389..79849
                     /locus_tag="OO322_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5905107.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            79883..80692
                     /gene="murI"
                     /locus_tag="OO322_000083"
     CDS             79883..80692
                     /gene="murI"
                     /locus_tag="OO322_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5905108.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(80811..81505)
                     /locus_tag="OO322_000084"
     CDS             complement(join(80811..81259,81259..81505))
                     /locus_tag="OO322_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5905109.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82024..83397)
                     /gene="eutA"
                     /locus_tag="OO322_000085"
     CDS             complement(82024..83397)
                     /gene="eutA"
                     /locus_tag="OO322_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5905110.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83372..83851)
                     /gene="eutP"
                     /locus_tag="OO322_000086"
     CDS             complement(83372..83851)
                     /gene="eutP"
                     /locus_tag="OO322_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5905111.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(83864..84199)
                     /gene="eutS"
                     /locus_tag="OO322_000087"
     CDS             complement(83864..84199)
                     /gene="eutS"
                     /locus_tag="OO322_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5905112.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84492..86771)
                     /gene="maeB"
                     /locus_tag="OO322_000088"
     CDS             complement(84492..86771)
                     /gene="maeB"
                     /locus_tag="OO322_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5905113.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87060..88010
                     /gene="tal"
                     /locus_tag="OO322_000089"
     CDS             87060..88010
                     /gene="tal"
                     /locus_tag="OO322_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5905114.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88030..90033
                     /gene="tkt"
                     /locus_tag="OO322_000090"
     CDS             88030..90033
                     /gene="tkt"
                     /locus_tag="OO322_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5905115.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90136..91179)
                     /locus_tag="OO322_000091"
     CDS             complement(90136..91179)
                     /locus_tag="OO322_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5905116.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91305..91880)
                     /gene="nudK"
                     /locus_tag="OO322_000092"
     CDS             complement(91305..91880)
                     /gene="nudK"
                     /locus_tag="OO322_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5905117.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(91948..93927)
                     /gene="aegA"
                     /locus_tag="OO322_000093"
     CDS             complement(91948..93927)
                     /gene="aegA"
                     /locus_tag="OO322_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5905118.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94133..95850
                     /gene="narQ"
                     /locus_tag="OO322_000094"
                     /pseudo
     CDS             94133..95850
                     /gene="narQ"
                     /locus_tag="OO322_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96052..99165
                     /gene="acrD"
                     /locus_tag="OO322_000095"
     CDS             96052..99165
                     /gene="acrD"
                     /locus_tag="OO322_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5905119.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99264..99323)
                     /gene="ypfM"
                     /locus_tag="OO322_000096"
     CDS             complement(99264..99323)
                     /gene="ypfM"
                     /locus_tag="OO322_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5905120.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            99704..100060
                     /locus_tag="OO322_000097"
     CDS             99704..100060
                     /locus_tag="OO322_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5905121.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100064..101191
                     /gene="dapE"
                     /locus_tag="OO322_000098"
     CDS             100064..101191
                     /gene="dapE"
                     /locus_tag="OO322_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5905122.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101219..101419
                     /locus_tag="OO322_000099"
     CDS             101219..101419
                     /locus_tag="OO322_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5905123.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101529..102227)
                     /gene="ypfH"
                     /locus_tag="OO322_000100"
     CDS             complement(101529..102227)
                     /gene="ypfH"
                     /locus_tag="OO322_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5905124.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102301..104316)
                     /gene="tmcA"
                     /locus_tag="OO322_000101"
     CDS             complement(102301..104316)
                     /gene="tmcA"
                     /locus_tag="OO322_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5905125.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104331..105194)
                     /locus_tag="OO322_000102"
     CDS             complement(104331..105194)
                     /locus_tag="OO322_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5905126.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105362..106075)
                     /gene="purC"
                     /locus_tag="OO322_000103"
     CDS             complement(105362..106075)
                     /gene="purC"
                     /locus_tag="OO322_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5905127.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106288..107322)
                     /gene="bamC"
                     /locus_tag="OO322_000104"
     CDS             complement(106288..107322)
                     /gene="bamC"
                     /locus_tag="OO322_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5905128.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107339..108217)
                     /gene="dapA"
                     /locus_tag="OO322_000105"
     CDS             complement(107339..108217)
                     /gene="dapA"
                     /locus_tag="OO322_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5905129.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108363..108935
                     /locus_tag="OO322_000106"
     CDS             108363..108935
                     /locus_tag="OO322_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5905130.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            108935..109405
                     /gene="bcp"
                     /locus_tag="OO322_000107"
     CDS             108935..109405
                     /gene="bcp"
                     /locus_tag="OO322_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5905131.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            109658..110275
                     /gene="hyfA"
                     /locus_tag="OO322_000108"
     CDS             109658..110275
                     /gene="hyfA"
                     /locus_tag="OO322_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5905132.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110275..112292
                     /gene="hyfB"
                     /locus_tag="OO322_000109"
                     /pseudo
     CDS             110275..112292
                     /gene="hyfB"
                     /locus_tag="OO322_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112303..113250
                     /gene="hyfC"
                     /locus_tag="OO322_000110"
     CDS             112303..113250
                     /gene="hyfC"
                     /locus_tag="OO322_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5905133.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113267..114706
                     /gene="hyfD"
                     /locus_tag="OO322_000111"
     CDS             113267..114706
                     /gene="hyfD"
                     /locus_tag="OO322_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5905134.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            114718..115368
                     /gene="hyfE"
                     /locus_tag="OO322_000112"
     CDS             114718..115368
                     /gene="hyfE"
                     /locus_tag="OO322_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5905135.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115373..116959
                     /gene="hyfF"
                     /locus_tag="OO322_000113"
     CDS             115373..116959
                     /gene="hyfF"
                     /locus_tag="OO322_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5905136.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            116949..118663
                     /gene="hyfG"
                     /locus_tag="OO322_000114"
                     /pseudo
     CDS             116949..118663
                     /gene="hyfG"
                     /locus_tag="OO322_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            118673..119218
                     /gene="hyfH"
                     /locus_tag="OO322_000115"
     CDS             118673..119218
                     /gene="hyfH"
                     /locus_tag="OO322_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5905137.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119215..119973
                     /gene="hyfI"
                     /locus_tag="OO322_000116"
     CDS             119215..119973
                     /gene="hyfI"
                     /locus_tag="OO322_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5905138.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            119966..120379
                     /gene="hyfJ"
                     /locus_tag="OO322_000117"
     CDS             119966..120379
                     /gene="hyfJ"
                     /locus_tag="OO322_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5905139.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120409..122238
                     /gene="hyfR"
                     /locus_tag="OO322_000118"
     CDS             120409..122238
                     /gene="hyfR"
                     /locus_tag="OO322_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5905140.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122239..122421
                     /locus_tag="OO322_000119"
     CDS             122239..122421
                     /locus_tag="OO322_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5905141.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122443..123315
                     /locus_tag="OO322_000120"
                     /pseudo
     CDS             122443..123315
                     /locus_tag="OO322_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123353..124414)
                     /locus_tag="OO322_000121"
     CDS             complement(123353..124414)
                     /locus_tag="OO322_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5905142.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            124627..126090
                     /gene="bepA"
                     /locus_tag="OO322_000122"
     CDS             124627..126090
                     /gene="bepA"
                     /locus_tag="OO322_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5905143.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126111..126470
                     /gene="arsC"
                     /locus_tag="OO322_000123"
     CDS             126111..126470
                     /gene="arsC"
                     /locus_tag="OO322_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5905144.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(126603..127349)
                     /gene="hda"
                     /locus_tag="OO322_000124"
     CDS             complement(126603..127349)
                     /gene="hda"
                     /locus_tag="OO322_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5905145.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127399..128688)
                     /gene="uraA"
                     /locus_tag="OO322_000125"
     CDS             complement(127399..128688)
                     /gene="uraA"
                     /locus_tag="OO322_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5905146.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(128774..129400)
                     /gene="upp"
                     /locus_tag="OO322_000126"
     CDS             complement(128774..129400)
                     /gene="upp"
                     /locus_tag="OO322_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5905147.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            129724..130761
                     /gene="purM"
                     /locus_tag="OO322_000127"
     CDS             129724..130761
                     /gene="purM"
                     /locus_tag="OO322_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5905148.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            130761..131399
                     /gene="purN"
                     /locus_tag="OO322_000128"
     CDS             130761..131399
                     /gene="purN"
                     /locus_tag="OO322_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5905149.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131571..133637
                     /gene="ppk1"
                     /locus_tag="OO322_000129"
     CDS             131571..133637
                     /gene="ppk1"
                     /locus_tag="OO322_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5905150.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            133642..135183
                     /gene="ppx"
                     /locus_tag="OO322_000130"
     CDS             133642..135183
                     /gene="ppx"
                     /locus_tag="OO322_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5905151.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135222..>136304)
                     /gene="pdeF"
                     /locus_tag="OO322_000131"
     CDS             complement(135222..>136304)
                     /gene="pdeF"
                     /locus_tag="OO322_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5905152.1"
                     /translation="SPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFF
                     WDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRE
                     LKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPGSFLPV
                     AHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAK
                     YQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNA
                     DLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDY
                     MQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34006 a        35076 c        33516 g        33706 t
ORIGIN      
        1 ggcagcatga gcctattgcg ctagcgctag gagacgataa tgacaaagac gggtgttttt
       61 caggtagtgc tgtcgatgac aatggtgtcc tctcacttat ctacaccgga cacgtctggc
      121 tcgatggtgc aggtaatgac gatgcaattc gcgaagtaca atgtctggct accagtcggg
      181 atggtattca tttcgagaaa cagggtgtga tcctcactcc accagaagga atcatgcact
      241 tccgcgatcc taaagtgtgg cgtgaagccg acacatggtg gatggtagtc ggggcgaaag
      301 atccaggcaa cacggggcag atcctgcttt atcgcggcag ttcattgcgt gaatgggcct
      361 tcgatcgcgt actggcccac gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact
      421 ttttcagcct tggcgatcag cattatctga tgttttcccc gcagggaatg aatgccgagg
      481 gatacagtta ccgaaatcgc tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac
      541 gactttttgc acaatccggg cattttactg aacttgataa cgggcatgac ttttatgcac
      601 cacaaagctt tttagcgaag gatggtcggc gtattgttat cggctggatg gatatgtggg
      661 aatcgccaat gccctcaaaa cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc
      721 tatcagagag caatggcaaa cttctacaac gcccggttca cgaagctgag tcgttacgcc
      781 agcagcatca atctgtctct ccccgcagaa tcagcaataa atatgttttg caggaaaacg
      841 cgcaagcagt tgagattcag ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg
      901 gattacagct cggcactgga atgcggctgt atattgataa ccaatctgag cgacttattt
      961 tgtggcggta ttacccacac gagaatttag acggctaccg tagtattccc ctcccgcagc
     1021 gtgacacgct cgccctaagg atatttatcg atacatcatc cgtggaagta tttattaacg
     1081 acggggaagc ggtgatgagt agtcgaatct atccgcagcc agaagaacgg gaactgtcgc
     1141 tttatgcctc ccacggagtg gctgtgctgc aacatggagc actctggcta ctgggttaac
     1201 ataatatcag gtggaacaac ggatcaacag cgggcaaggg atccgcgtca ctcttccccc
     1261 ttcacgacct tcaataatat gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg
     1321 aattgtggtc agcggcggta aaaacagatg cccgacgcca accagattat caaagcccat
     1381 tacagcgaca tcctgcggga tacgtacccc cttcgccaaa agaacctgat aagccacaaa
     1441 ggctgcgcga tcgttaccac atatcagaac atcaaaatct ggtttgcccg atttgaagtg
     1501 agcattgagt aaacttgcga gatcggtgta gtgatcatca cctgttgcca tgtgaaattg
     1561 tttcacctca gccagatctc gtccagcatc acgccaagcc tgctcaaatc cctgccgacg
     1621 ataccctgtt gccaacgcac tttccggtag ccagaagcat aatggttgac gatagcccgc
     1681 cgcgagcaaa tgctgtgttg attcatattg tgcagtgtaa tcatcaggga tataactggg
     1741 taacgctggg tcatccgcca cacagttcgc caatacaata ttttcaccat acagagactc
     1801 aggcagcgtg atatgtcgca gccccattgt agtatagata atgccatccg gacggtgggc
     1861 aagcagctga cgtgccgcgc gggcagcgtc atcttcagaa aaaatattga ttaaaaaact
     1921 attccagccg aactcgctgg cggtttgctc aatggcaagc agaatatcaa cagagaaagg
     1981 agtggtagcc gtgtcctgcg ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg
     2041 catcttacgg gcggaaagat caggaacata attcagggtc tggattgcct gcaatacgcg
     2101 gtcacgcgtt gcaggacgca cagattctgc attatgcatc acccgggaga ctgtcatcat
     2161 cgacactccc gccaggcgtg cgacatcctt taatgaagcc atacccaagc cgtttgccgt
     2221 aaaacgggca ctgtagcaga aacagacgtc actggcgaga tccaacgccc tatcacctga
     2281 cacagcaata caataaaaaa taacaataat tcccggacaa ttgtccccaa ttccgcctct
     2341 gttctcgcca acgagtaacc atataggcaa gtaagtaaaa catgccaact ggcatcgcca
     2401 acctggagaa gaaaatataa atatggatca ttgtgctgcc actgtatccc gacatcagcc
     2461 cggaaattat tgcgattgct atcggttcag gtgcaattgg ctgcacgatc gttacggact
     2521 cgcttttctg gctggtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact
     2581 atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat
     2641 ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc
     2701 ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctag
     2761 cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga
     2821 cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttg ctgaaacagc
     2881 tgccactggg gggattattg aatcagaact ggttgccatt ccggctatgc aaaaacggct
     2941 ggaaaaggaa tatcaacaac cgatcagcgg gcaactccta ctgaaaaaag atagccattt
     3001 gcccatttcc ggctctataa aagcacgcgg cgggatttat gaagtcctgg cacatgcaga
     3061 aaaactggct ctggaagcgg ggttgctgac gcttgatgat gactacagca aactgctttc
     3121 tccggagttt aaacagttct ttagccaata cagcattgct gtgggctcaa ccggaaatct
     3181 ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag ttcatatgtc
     3241 tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga
     3301 atatgagcaa gattatggtg ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc
     3361 gaactgtttc tttattgatg acgaaaattc ccgcacgttg ttccttgggt attccgttgc
     3421 tggccagcgt cttaaagcgc aatttgccca gcaaggccgt atcgtcgatg ctgataaccc
     3481 tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcgggct
     3541 taaactggcg tttggcgatc atgttcactg cttttttgcc gaaccaacgc actccccttg
     3601 tatgttgtta ggcgtccata caggattaca cgatcagatt tctgttcagg atattggtat
     3661 cgacaacctt accgcagcgg atggccttgc agttggtcgc gcatcatgct ttgtcgggcg
     3721 ggcaatggag cgtctgctgg atggcttcta tacccttagc gatcaaacca tgtatgacat
     3781 gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa ccttcagcac tggcgggtat
     3841 ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc
     3901 agaacaattg cgtaatgcca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga
     3961 agaagagatg aatcaatatc tggcaaaagg ccgttaataa cgtttcaacg caacatcgca
     4021 atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc cggtcttacc
     4081 cgctataacc ccctcctttt atcatcaccc aacgcctttc gctgtaaacg gcggtttcgc
     4141 aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt
     4201 tgcagaaata gaaagtgact gctgggttat ttcattatta atttcattaa gaactcctga
     4261 aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaacacgg ggttaaattg
     4321 atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc acagcgtcat
     4381 tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca tactcgaggc
     4441 attggcaaat ttattatctg gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa
     4501 acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt
     4561 tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactgaatgt
     4621 cactaacacc cgcatgtcta ttttattgcc ataacgtcct atcaaaggtg aaatcaatag
     4681 tggcatgatg ccgatgggcg cataagcaag cccggcccat atcgcattat accccatcgt
     4741 tttctggagt aactgcggca taaggacgat cgctccagag taaaataaat acgcgcaggt
     4801 gatactcaca ataccaatgg tgaaattacg ggacttaaac aaactgagat caagaatcgg
     4861 gttctctgag gtcgattccc aaatgactaa agagatcaga gaaataactg atactactgt
     4921 taatattatt attgtactcg agttgaacca atccagatcg cgccctttat caagcataat
     4981 ttgcaagcca ccaacaccga gcactaacag ggtcagtctt ggtagattca tttttaccgg
     5041 tgaagtttca gtttctcttc ctttaagtaa ggttaagcat aatgtcagga cgataatccc
     5101 catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa tatagccgcc
     5161 caatatcggc ccacatatcg gagcgataat cacggtcatt gaccataatg ccagagcaaa
     5221 tgttcttttt tctggtggat aattccttaa taataaactc tgtgacagtg gaattaacgg
     5281 ccccgccatt aatccctgaa cgactctaaa aaatatcagc acatcaagat tggtcgataa
     5341 actacacatt aaggaagaca gcgaaaaaaa agtgactgaa agtaaaaata accttaattc
     5401 gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa
     5461 cgaggtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg
     5521 tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta atgacaatgc
     5581 aatagtaacg caccataacg tcccaccggt taatggtgcc ggagttgatt tagtgattgc
     5641 cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat cggactggta
     5701 tcgataacta aagccttact ggtataagca ggcatggagg tcacggttga agccagatca
     5761 ggcatctcag caatgtcttc gtccttcgta tcaatagttg cagtcatcga taaaccaatg
     5821 cgcaaggggt gttccatgag ttcttttgga tcaagagaaa cttcaaccgg tacacgctga
     5881 acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg
     5941 gttcccatat tgatccctgt cacccgacca tgaaacacaa cattttcacc ataaagatcg
     6001 ctgataatat tgactgattg accaatccgt acatccgtga gttgtgtttc tttaaagttg
     6061 gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc ggaactcact
     6121 gtttcgccga cctgaacact tcgctgggca atatagccag taaccggact ccttatatcc
     6181 gtacgtttaa gcaccaacca ggcttctttg gttgcatccg ccgcttcaac tacttgtggc
     6241 tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc ctggatagcg
     6301 gcattcagtg ccgctttgct acttattaac gtatctttgg tatgctccag cgtttctttt
     6361 gaaataaccc cctgcttcgc taaaggcaca cgacggttat aatcttctaa agattgttga
     6421 tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtacagttta
     6481 ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc gatagtggca
     6541 tcagttttat ccagtgaaac taaaatgtca ccctgtcgaa cgtagttcgt atctttatga
     6601 ttaactacag tgacactacc tgagacttgt gcagaaattg gatctgcatt ccccgtgaca
     6661 taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc ataggcacct
     6721 gaaaacgcaa taaataaaac tatcaccaat aaagaaaagt atttttttct gttagactgt
     6781 tttttatttg aattaatctg ttccactatt atctctcatt tcgcatagat gaaatttatg
     6841 aattgaatga aaagagtatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat
     6901 cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac
     6961 cgtggctttt gcaatacaat tcttacgcct gtaggattag tgagaagact tatagtgcta
     7021 acttgaaact ataaatcatc ggtataatcc ctgattttat tgttgacatt tcatttatgc
     7081 cgactattta tatggtatac ttgtcgaatt atcttaaagg aaactcagat tttcttattt
     7141 ttattgagaa aatgagatga cgccttatgt ctgtattact acagggagaa gggagatgct
     7201 tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc
     7261 gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga gttaactgaa
     7321 ggcggaagcg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat cattgatgtc
     7381 gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc
     7441 ggaattatta ttatcgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat
     7501 gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga acaatatcat tgcggctatt
     7561 gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt
     7621 ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg
     7681 tatattcttg atggcaagga taataatgac attgcggaaa aaatgttcat cagcaacaaa
     7741 actgtcagca cgtataaaag tcgcctgatg gaaaaattag aatgtaaatc actgatggat
     7801 ctttacacat tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt taccctattt
     7861 ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacgaag attacatcga
     7921 atatcgtggc atcagtagta acaaccgtgt cacacttgat ccagtacgtc tgagcaacaa
     7981 ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac
     8041 ggctaccttg ttgcataccg attcgcagca acaggttcgt ggtattaatg ctgattattt
     8101 aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca
     8161 aaaagcaatg gacgcgcttg cagaaggtga agtcgatata gtgttatcac atttagttac
     8221 ttcgccgcct cttaataatg acattgctgc aaccaaacca ttgataatta cctttccggc
     8281 gctggtaacc acccttcacg actcaatgcg accgcttacc tcaccaaaac cagtaaatat
     8341 tgctcgggta gcaaattacc ccccagacga ggtaattcat caatcatttc caaaagcaac
     8401 aattatctct tttacaaatt tatatcaggc attagcatcc gtctcagctg ggcacaatga
     8461 ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgctatt tcactcactc
     8521 cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat aattttttct tgaccagaaa
     8581 agaatctgtc attcttaatg aagtactcaa tagatttgtt gatgctttaa caaatgaagt
     8641 tcgctatgaa gtatcacaaa attggcttga tacaggaaac ctggcctttc tgaacaaacc
     8701 attagaactc actgaacatg aaaaacagtg gattaagcag catcccaatt taaaggtgct
     8761 ggaaaatcct tactcgcccc cctattctat gacggatgaa aatggctcgg ttcggggcgt
     8821 tatgggggac attcttaata ttattacctt gcaaacaggt ttaaattttt ctccgatcac
     8881 cgtttcacac aatatccatg ctggaacaca acttagcccc ggaggatggg atataatacc
     8941 tggcgctatt tatagtgaag atcgagaaaa taatgtttta tttgctgaag ccttcataac
     9001 aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg
     9061 aatgaaagtt gccattccat attattatga gctgcattcg caattaaaag agatgtatcc
     9121 ggaggttgaa tggatacagg tcgataatgc cagcgctgca tttcacaaga ttaaggaagg
     9181 tgaacttgat gctctggtcg cgacacagct aaattcgcgt tacatgatcg atcattacta
     9241 tcctaatgaa ctttatcatt ttcttattcc tggcgttccg aatgcatcgc tttcgttcgc
     9301 ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga atgcaattcc
     9361 cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa atgcccaatg tgaccattga
     9421 cacatgggac ctatatagcg agcaatttta tattgttacg acattatccg ttttattagt
     9481 tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc gtaaagtcat
     9541 tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca ctctcggatt ccttaccgaa
     9601 tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cataatagtg cttttgaaca
     9661 ttatttcact gcggattact acaaaaatgc aatgttacca ttagaaaaca gtgattcacc
     9721 ctttaaagat gttttttcta atgcgcatga agtcacagca gaaacgaaag aaaatcgaac
     9781 aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca ttaatcactg
     9841 gcatacatta tgcaatcttc ctgcaagtga caatgcagta tatatttgtg gttggcaaga
     9901 tattactgaa acgcgtgatc taattaatgc actcgaggta gaaaaaaata aagcgataaa
     9961 ggctaccgta gcaaaaagtc agtttctggc aacgatgagt cacgaaataa gaacaccaat
    10021 aagctctatt atgggcttcc tggaacttct gtcgggttct ggtcttagca aggagcaacg
    10081 ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct taattggtga
    10141 aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac aatgggtcga
    10201 tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg caagcaaatc
    10261 gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga tcgaccctca
    10321 ggcgtttaag caggtcttat caaatttact gagtaatgct ctcaaattta ccaccgaggg
    10381 ggcagtaaaa attacgacct ccctgggtca cattgatgac aaccacgctg ttatcaaaat
    10441 gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac tgtttaagcg
    10501 ctacagccaa acaagtgcag gtcgtcagca aacaggttct ggtttaggct taatgatctg
    10561 caaagaatta attaaaaata tgcagggcga tttatcatta gaaagtcatc caggcatagg
    10621 aacaacattt acgatcacaa tcccggtaga aattagccag caagtggcga ctgtcgaggc
    10681 aaaagcagaa caacccatca cactacctga aaagttgagc atattaatcg cggatgatca
    10741 tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg atgttgatga
    10801 agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg atctgcttat
    10861 tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac tccgtgagca
    10921 aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg aacgtgaaaa
    10981 agggttaagt tgcggcatga acttatgttt gttcaaaccg ttgaccctgg atgtactgaa
    11041 aacacattta agtcagttac accaggttgc gcatattgca cctcagtatc gccaccttga
    11101 tatcgaagcc ctgaaaaata atacggcgaa tgatctacaa ctgatgcagg agattctcat
    11161 gactttccag catgaaacgc ataaagatct acccgctgcg tttcatgcac tagaagctgg
    11221 cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta acatcctgaa
    11281 tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgctt cagatgacag
    11341 taagcctgaa attcttcagc tgctgaactc tgttaaagaa cacattgcag agctggacca
    11401 ggagattgct gttttctgtc agaaaaatga ctaaattgcg gctcccacaa tgttctaatg
    11461 tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg aatttgttcc
    11521 ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca gccgctgatt
    11581 tttatcggat tacccggcat cattattcct ccggcttcaa tcaacatatt tctcgcctga
    11641 gtttgtggca aattaatggc ctcagccaca cttaataacg gcgcgacggg cacaccaact
    11701 tcatgtattt tggctaacca aacttcagct gcctgcgttt ttaacgttcg ctcaatatat
    11761 tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatcg gggatcatta
    11821 accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc attaccacaa
    11881 caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat gtagggatgg
    11941 cgatttccca ggcgttgtgg tgacttccct gtcgcaatat atgccatcag accatgctcc
    12001 agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct ctggctcttt
    12061 tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc gcatagatcc
    12121 gcaagagagg taccaacacg cactggcgga gcatcagggt accctgtttc catcattatc
    12181 ccactcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg accggtatgt
    12241 ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgtaa cctttcccat
    12301 gaaaacccca gtttttccat tgtacctagg cgaaaattct cagctaatac atcagcttgt
    12361 ttgagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag aaccacactc
    12421 tctttgccat gattaataaa actgtaatag agtgactgtc catccacata gggaccaaat
    12481 gtgcgggtat catcaccatg acctggtggc tcaactttaa ttacccttgc gcccatatta
    12541 taaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat aactaataag
    12601 ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga aaaaagccga
    12661 aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt atgaaaccag
    12721 tcgactgaca taaatccaca agggagccgt gacgacaaag cccagtacgc tcacagccaa
    12781 tgacgctgta ccagtacggg tgtagacatt aaaccggctg gcaataatga ttccggagaa
    12841 tgccggcggt aatgcgcctg ccagtaccat catctgcaaa tgttcgctgt tcaaatgaca
    12901 tgccatacca acgagaagca gcgccagtgg catcagaatc aacttcagga aggtgttata
    12961 agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg cagcgaatac
    13021 cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg ctgccggaat
    13081 ttttacccca accagcacca ggatcgttgc cagaacaggt gcccatacta ctggctcctt
    13141 tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc ctgaggaagg
    13201 attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag aaataattgc
    13261 taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg caaaccccag
    13321 gaatccaatg gtaggtgaac ccgcaattaa tgcacataca gctgcttctg catgggtacg
    13381 tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa catccgacaa taaccacaag
    13441 tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc gggtaataga
    13501 aacaaataga gccgcaggaa gagcgtagtt taataccagt ttattaaatg cgcgggcttg
    13561 atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga ttacgataat
    13621 aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa gggtcccctc
    13681 accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac cagcgacttg
    13741 ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt cgataaccac
    13801 attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac gaagttcatc
    13861 tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt cataccttgc
    13921 atggtgcaac agatccgttg gtgatggtgc accagcgcca ctgagatcaa caccgtctcc
    13981 tctgtagatg ccgccattat taaaaataac gatcgtcacc ggcaggttat atcgacaaat
    14041 cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg cgacaaccgg
    14101 agaaccagaa gtcacgctag caccgatggc atagcccata ccgatgccca tgacacccca
    14161 ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc gtgcattatc
    14221 cagggtattt gcaccttcat taactaaata aatatcctgg ttctcgcgca atacgtcgcg
    14281 cacagcactt aatgcattaa agtaatttaa tggttgagta tctgtactta atttttcatg
    14341 cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata ccagtggagt
    14401 cgtaaatgtg ttttgtttca gttctgccag cataccttgc atactggatg caatatcacc
    14461 aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa tatccagttg
    14521 aataaactgt gtatctgccg cccatccttt tttaccgtgt gccaataacc aattcagtcg
    14581 tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg cagctgccgc
    14641 agaaagtgga tgcgtatctt caaggatccc tttcgccata gacattggca ggaatggaat
    14701 ctgagcactt tcaataaatt cacgaagctg ttcatcagct tgtgaatacg ccgcgccttt
    14761 gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc tagtgactga
    14821 cttcgggcat ggcaataatg ctggcgacgg attttcaact ttaacaatcg tggttaacgc
    14881 ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat aaactccgcc
    14941 agggcgaccc gatacagaga cccggatagc gcgtgccaat gcaatgccaa gatcctgcgg
    15001 ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt ggtccagctc
    15061 ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc taatcatgat
    15121 aatcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga ggaatcctgg
    15181 cgcagaaact gtcaggcaga tccccggttt ttgggtaaga aaaccgcttg ccgcagcggc
    15241 atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg cctgggcatg
    15301 gcgtgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa tattattctg
    15361 ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat ctgacatgat
    15421 taataacctc gaaattttta taaattggga gccagaagcc cccgttaata tcagatggcg
    15481 tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg caatacagca
    15541 gcggtatgtt cacctaatag cggcgcagct ttaatatccg gcgtaaaggc agagaatttc
    15601 attggacaac caacggtcag atattttcca cgcaacggtt gttccacttc aacaacactg
    15661 ccactttggc gcaaagaggg atcaagtgaa atttctttca tacttaaaac cggtgcacaa
    15721 ggaatatcaa actgagtcaa ataggccacc gcttcatgtt tatcaatagt gacagtgtat
    15781 ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gggcatgggc tgtactgtat
    15841 gccggatcgg taatccattc tggtttgccg atggctttac aggtgttttc ccagttttgc
    15901 tcctgaatag tgaaataaat ataggcgtta ggatcggttt cccagccttt acatttcagg
    15961 atccagccag gctgaccgcc accacctgca ttaccaccgc ggggaactgc atcaccaaat
    16021 gtaccattcg gatactgcgg gtattcttcc agataaccca atttatcgag acgctgctgg
    16081 tcacgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat ggtgactcgt
    16141 tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat cagcaaatgc
    16201 attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc atcccaaaaa
    16261 cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc ttttacattc
    16321 acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg tggattgatt
    16381 tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg aaagttctcg
    16441 actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc cgctgttttg
    16501 gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag cgcatcgata
    16561 tcaggaatat ctcgcagttg gtgacgcgtt acgtcaccaa cgccgggacg ttcaatttta
    16621 ataacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga ttgcacaccg
    16681 gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc tattagttaa
    16741 ttaaatatgt gttaatttag cgtttattta aggcataacg ccctctataa ataatctgca
    16801 aaatatcttc gtagcggcac tcgatatttt taaattcctg tatcaagtac tgaaaattat
    16861 tttatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa agaagaatat
    16921 gataaatatt aattcgtggt aatcagagag agtcgcgaca tttgtgatgt tattcttaca
    16981 actgtaagaa gcgccggtga tttacaagta agagtatgat tacgcttttt aaagcgtacc
    17041 ataaagcata acataataaa tgatttgtga tgacacggga ttattgcggt ttactttgag
    17101 gaaaataaaa tgcgtgttta tagaaccgcg agcgccaaat aattttatga ccacaacctg
    17161 tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta cctcaatata
    17221 ctgcctgtca gcattagttc acaaacagtg cttcgctatc gacgatgatt ccatcttccg
    17281 cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat
    17341 cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc attacgccaa
    17401 caccggccag gcgaagctct tccattgcgc cctttttatc ccctttcgcc attttttcat
    17461 tggcaatttt tatcgcggct tctttttcgg gtgttgctac ataactctca gaaattccga
    17521 cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta cctggcttag
    17581 cgaactttgc ccattcggta ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt
    17641 cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca
    17701 tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgctgag
    17761 tttgctgttg tgccgctacc ggtgcattat cagcagccca tacagttgaa gatgccagaa
    17821 ttgctgtgac catcgtagcc ataattaaac gtttcatact tacctcattc tttaacagtt
    17881 ttagaaaacg ccgccatcat attgggtgag catgaaatga gaaatcagag agcaacggtc
    17941 attttgaaag ggttatctta caattgtagt tattatcgca aagcggcatt tagtcttaca
    18001 gttgtaggaa tatgcctcct gtcgcagaga actattctta tataccacaa agagatgtca
    18061 tttattatat tgatgtaaat attgattaat tatatgtaaa tacagccata acatcgtatg
    18121 gttctcagtg aaccattttc aggataactt tctatgaaag taaacttaat acttttcagc
    18181 ttatttttat tggtctctat tatggcatgc aatgtttttg cattttccat ttctggtggt
    18241 gtaagtgaga ggagctataa agagactgaa aaaacatcag cgatgacgac cacacactct
    18301 acaaaacttc agccatcaca ggcgattttg tttaagatga gagaagatgt gccaccatta
    18361 aacctcacag aagaaatcac gcctacttat ccgacaaagg cgaattatct tattcatcct
    18421 gtgcgatagc gtctgtcaca tggcaggtga acaatcatgt ctgatgctga tgtgaacacc
    18481 tgcccaaatg agccatcctg acccactatc gcctgccata ccccaccata aagcgaatca
    18541 tcaagccaca agccggggca agaaacataa atatccccgt caccagttca ttaccgccag
    18601 ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat caccatgccg aagaaagaag
    18661 agaccgccga taccacgttc agtacctgac cgatgtagcc gcttacgcag acaatacaca
    18721 gggcgagaaa catccataaa taaatcatag ctcaacctcc tgccgcgcta aaaaacctgt
    18781 aacttcagtc actttaatag taagacaatc cttaacctct ggcaattttt gctaagcatc
    18841 aatgaaaaca gcacgttaaa tgcaacgcta ttgcgtgcgg aaaatttttt actttgcacg
    18901 attaataatc agtagctgaa agcagtcagc cagtgataaa gtagagccac cgcattcatg
    18961 ctcctcgtgg ttatgtcctg actagtcttt acactcttta caggaaccat tgtcgtacat
    19021 gatggcccaa ccaattgagt gtaccgctgt cgacatggaa tcttctgcga tacaacaatt
    19081 cgtatctaca gaaggtaact atgtttccac aatgcaaatt ttcccgcgag tttctacatc
    19141 ctcgctactg gctcacatgg tttgggcttg gtgtactctg gctttgggta cagcttcctt
    19201 atcctgttct ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca ttcctgaaac
    19261 gtcgtgaatc tatcgcccgt aaaaacctgg aactttgttt cccgcagcat tctgcggaag
    19321 aacgcgagaa gatgattgcc gaaaactttc gttcactcgg catggcgctg gtagaaaccg
    19381 gcatggcatg gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt gaagggttgg
    19441 ataaccttaa acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc gtccatttta
    19501 tgtcgctgga actgggcggc cgcgtgatgg gactgtgcca accaatgatg gctacctatc
    19561 gtccacataa taatcagctg atggaatggg tgcagacccg tgggcgcatg cgctctaaca
    19621 aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg tgcactgaag aaaggtgaag
    19681 cggtatggtt tgctcccgat caggattatg gtcgtaaagg cagctccttc gcgccgttct
    19741 ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt ctctctggcg
    19801 cagccatgtt gaccgtaacg atggtaagaa aagcggatta cagcggatat cgtttgttca
    19861 tcaccccaga gatggaaggc tacccgacag atgaaaatca agccgctgct tatatgaaca
    19921 agattatcga gaaagagatc atgcgcgcac cggagcagta cctctggatc caccgtcgct
    19981 ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta acaaactgct cttactcttt
    20041 ccctcctcaa atcggccata ataacctcat gatttttata gcttttttct taagtttttt
    20101 ccttcgctga atatctaatt attaccgtca gaaattaaac tgtcgccgat atgagacact
    20161 tgtaagttac taaaagtaaa taagaaaatg cctcttgtct cgtttcagtt ttaggcgtac
    20221 attagcgccg tctcgagcag tttagctcag aattctgggg tgatgcccac ggtaaaatac
    20281 agggctttta aggtcgcttt gcccggtgtc aactcactgt atcaggtgta atgaagtcat
    20341 tcaggcgtaa cagtaattac gcggagagat gtaaagtgaa atatttcttt atgggcattt
    20401 cttttatggt catcgtttgg gccggtactt ttgccctgat gatctaaaag caaactgtgc
    20461 aatgaaaaac aggagccatc aggctcctgt ttgcatttct gacgacggtg tttattccgc
    20521 gttttcgtga atatgtttgc tgctgacggg tagtaaaccg tcggcacgga acatcgcttt
    20581 aatccctctg atggcctgac gaatacggtc gtggttttca atcagtgcaa agcgaacatg
    20641 cgtatcacca tagtcgccaa agccaatccc cggcgagaca caaaccttcg cttcgttaag
    20701 gagcttcttg gcaaattcca gcgatcccat ggccgcatat ggttccggga ttttcgccca
    20761 gacatacatc gaagccttcg gcatttcgac catccagccc gcttcatgca gccctttaac
    20821 caatacatca cgacggcgtt tgtactgttc ggcaatgtcg cgcacgcact gttgatcgcc
    20881 ctcgagtgcc gcaatcgccg ccacctgcaa cggcgtaaaa gtgccgtaat cgtgatagct
    20941 tttgattcgc gccagggcac tgaccagcgt tttgttgccg accataaagc cgatacgcca
    21001 gcccgccatg ttgtagcttt tcgacagcgt aaagaactcg accgccacat cgcgtgcacc
    21061 cggcacctgc ataattgacg gcgctttcca gccatcgtag acgatatcgg cataggccag
    21121 gtcatggacc accagcacat cgtagcattt cgccagcgcc acaacctttt cgaagaactc
    21181 cagttccacg cattgcgcgg ttgggttcga cgggaagccg aggatcatca tcttcggttt
    21241 cggataactt tcacgaatgg cgcgctccag ttcgttgaag aaatcgacac cttccaccag
    21301 cggcactgag cgtacctgcg ccccggcaat caccgcgcca taaatatgga tcgggtaact
    21361 tggattcggc accagcaccg tgtcaccatg atccagcgtc gccagcatca gatgcgccag
    21421 gccctctttc gaaccaatag tgacgatggc ttctgattcc gggtcgattt caacgtcgta
    21481 gcgatcctga taccagcggg aaatggcgcg acgtaaccgc ggaatgccgc gtgaagtgga
    21541 gtaaccatgc gtgtccgggc gctgggccac agtacataat ttttcgacga tatgcggcgg
    21601 agtcgcaccg tccgggttac ccatgctgaa atcgataata tcttcgccgc gccgacgcgc
    21661 agccattttc agttcagcgg tgatgttaaa aacgtagggc gggagacgat caatgcgcgt
    21721 aaagcgacgt tcagggcgag tgtcagccat aatttcctca gattaacgtt agcgcccgga
    21781 ccgtccgagc gacgctgcca caatggtggc tgttttgaaa atagcctgat taatttctgt
    21841 ctgtccagcg ttttagaaca tttttatcaa tggatttaaa tgaggaactc cacttcaaaa
    21901 acaggaaatt tttctacgct aacaagaaaa tcaccaccaa ttttattaac attataattt
    21961 caataaattt accagccaga agtgcattat caagagatac attgctaata caccatttat
    22021 cttttaatcc ccttttaaat ttatggtttt tcctcaaaaa cggcctgctg gtcatcagcc
    22081 ttcaggttac ctatcataga ggtttaatcc ttattcagag tcacccgtgc acgaaatatt
    22141 caacatgctg ctggcggtct tcgatcgggc agcgttaatg cttatctgcc tgttctttct
    22201 catccgtatc cgcctgtttc gcgaactgtt gcacaagtcg gcgcactccc caaaagaatt
    22261 gctcgccgtt accgccattt tctcgctgtt cgccctgttc agcacctggt ccggcgttcc
    22321 cgtagaaggc tcgctggtga acgtacgtat tatcgcggtg atgtccggcg ggattctgtt
    22381 tggcccgtgg gtaggcatca ttaccggcgt gattgcgggt attcaccggt atttaattga
    22441 tatcggcggc gtgacggcga tcccctgctt tatcaccagc attctggcgg gttgtatatc
    22501 aggctggata aacctgaaaa tccccaaagc acagcgctgg cgctggatat cgccaacaaa
    22561 acgctaccgc tgtttcgcca tgtcaatagc gagtcattac gcaaggtctg cgaaattatc
    22621 cgcgatgaca ttcacgccga tgcggtggcg attactaata ccgatcatgt gctggcctat
    22681 gttggcgtgg gtgaacataa ctatcagaat ggcgatgact tcattagccc gactacccgt
    22741 caggcgatga attacggaaa aatcatcatt aaaaacaatg atgaagccca ccgcacacca
    22801 gagattcatt ccatgctggt gatcccattg tgggagaaag gggtcgtgac cggaacgctg
    22861 aaaatttact actgccacgc gcatcagatc acctcgtcat tacaggaaat ggcggtcggt
    22921 ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg ccgagcagct acgtgaaatg
    22981 gcaaataagg cagagattcg cgccctgcaa agcaaaatta atccccattt tctgtttaac
    23041 gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg ataccgctcg ccagttgatc
    23101 tttaatctgt cgcgttatct gcgctataac attgaattaa aagacgatga gcaaatcgat
    23161 atcaaaaaag agctgtatca aattaaagac tatattgcca ttgagcaggc ccgctttggc
    23221 gacaagctga cggttatcta tgatattgat gaagaggtga attgctgcat tcccagcctg
    23281 ctgatccagc cgttggtgga gaacgccatt gtccacggta ttcagccttg caaaggtaaa
    23341 ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg tacgcattgc ggtgcgagat
    23401 accgggcacg gcatcgttcc aaaggtgatt gagcgggtcg aagcgaatga aatgccgggc
    23461 aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt tattgtatgg cgaggggctg
    23521 catatccgcc gcctggagcc ggggacggaa attgcgtttt acattcctaa ccaacgcacc
    23581 ccagtcgcct cacaggctac gttattgctt tgagccggag tgatattgtg aaagtcatca
    23641 ttgttgaaga cgaattcctg gcacaacagg aactgagctg gctaattaaa gagcacagcc
    23701 agatggagat tgtcggcacc tttgacgacg gtctggacgt gttgaagttt ttgcagcata
    23761 accgcgtcga cgccattttt ctggatatca atattccgtc gctggatggc gtgttgctgg
    23821 cgcaaaacat cagccagttc gcccataaac cgtttattgt gttcatcacc gcgtggaaag
    23881 aacatgcggt agaagcgttt gaactggagg cgtttgacta cattctcaaa ccgtatcagg
    23941 agtcacgtat taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa cagcagacca
    24001 gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac cattaatctg gtgaaagatg
    24061 agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac gagaaaatga
    24121 cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc gaattttgca
    24181 gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc aatctgaaca
    24241 aaatacgcga aatcgaaccg tggtttaata acacctacat tctgcgactg aaagatctgg
    24301 attttgaagt gccggtcagc cgcagcaaag tgaaagaatt tcgccagtta atgcatcttt
    24361 aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt ttcgccgatc
    24421 tgttcagcgg cctagtgctt aacccgcaac tactggggcg cgtctggttt gccagccagc
    24481 ctgcctcgtt accggtgggc agtttatgta ttgattttcc ccgtctggat atcgtgctgc
    24541 gcggcgaata cggcaacctg ctggaagcaa agcagcagcg aatggtggaa ggagaaatgc
    24601 tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa caaaccggtg atgctgttaa
    24661 gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta cgatagccgc accacgtcgt
    24721 tgttgcatcc tgctcgccag acccagcttc ccagcctgca acgcggtgaa ggtgaagcga
    24781 tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcagaatatc attcagccac
    24841 tggtgttaag tttgctgcat ctttgccgca acgtggtgaa tatgccgccg ggtaattcac
    24901 aaccgcgcgg cgattttctc tatcacagca tttgtaactg ggtttaggat aattatgccc
    24961 agccgctcac ccgcgagagc gtggcgcagt tttttaatat cacgcccaat catctgtcaa
    25021 aactgtttgc tcagcatgga acgatgcgtt ttatcgagta tgtgcgttgg gtgcgaatgg
    25081 cgaaggcgag gatgattttg cagaaatatc atctgtcaat tcatgaagtg gcacagcgtt
    25141 gcggttttcc ggatagcgac tatttttgtc gcgttttccg gcgtcagttt agtctgacgc
    25201 cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct gtttccagca
    25261 gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag tttttattca
    25321 ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg ttagcaccca
    25381 gcgtcagcat gatcaccagt tcgacttcgc ccatttctga ctgccagtca accggtttcg
    25441 ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg tgcgggatcg
    25501 ctacgccaaa accaacgccg gtggtaacaa tctcttcccg ctgccagaca tcttcttcca
    25561 gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt gccgcacagg aactggatcg
    25621 cctgctcttt attgctaaaa tcctgatcaa caaagatatt ctccagcgcc agcagtgggc
    25681 gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc aatttcctgc gcactgcggc
    25741 attcacatgc ctgacgcgcc agttcccgac acgcctcgct atccagttga cgaagctggc
    25801 ttttcaccgc cggaatacgc gggctactca tactcagctc atccaggccc agcccaagca
    25861 gtagcggcag ataacggctt tcaccgccca gttcaccgca aatgcctacc catttgcccc
    25921 gctgatgcgc agtggtaact atttgctgca acatgcgcag gaacgatggc gtaatcgggt
    25981 tatatagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac tgggtcatat
    26041 cgttggagcc gatactgaag aaatcgacct catcgcagaa gtggtcgatg atgtagcaca
    26101 ccgacggcac ttccaccatg atcccaagcg taatcgtctc tgcatgacgc aggccatcgc
    26161 gcttaagctc aacgatcgct ttttggatct cgcctttcac ccataagatc tgatcgaggc
    26221 tgtgaaccat cgggatcatc aactgggcgt tgccgaaact ggcggcgcgc aaaatggccc
    26281 gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg cgatagccga
    26341 ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg ccgccgatgt
    26401 ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag tagcacctgc tggtaggctt
    26461 caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt tcggtacgga
    26521 acagcccgac accttccgcg ccgttggcaa acgcgcctgg cgcttccaga gcggtgccga
    26581 tattcgccgc aatatcaata cgcttgttat cacgggaata ggccagctgc gcggcagcct
    26641 gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc gacctgataa taaccgctca
    26701 ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcact gcaggttgcc
    26761 cggcataacg ggcaatcgca tccagcggca agccactcag taccgggatc gccgaggcac
    26821 gggccagaat cagtgtatgc gaggtgcgcc cggttttctc cagaatcata cccgcaagat
    26881 ttttcaaatc aaggctcaaa aactggcttg gggttaaatc ttcagccacc agaatggtcg
    26941 gtttttccag caccagattg ttgcgcggct tcagttccgg ccaggtgata tgcagcaact
    27001 gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag ataatcgctg gcagaagcag
    27061 atagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagt ccctgatgct
    27121 gttctgtcat caggcgacgg atattgcctg caaattcatc atcctgaatc agcgacaaat
    27181 gagcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctga ttcagttgct
    27241 cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg gcggggattg
    27301 cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg ccgacgccgc
    27361 ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa cgcggcagcg
    27421 gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc gctgtcgata aagcgcacca
    27481 ggatgtactc ttccagcacg cgccgcgcct gctcttcatc gctgccgcta atgttcaggc
    27541 tgcaactgtc attaaataag gtgccagttc caatcaacgc cagcgagctt ttagcatcag
    27601 cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct ttgccactgg ctgcactgtt
    27661 ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag agaaattgaa
    27721 tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc agttgaacaa
    27781 ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc tgcaaaatgt
    27841 cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc cccattacca
    27901 atgtgggtac gccagtaccg gttaagtgca ccgctccacc gtccgtgccg ccgttactga
    27961 acatatccgc ctgcaacgga acgccaattt ctgccgccac ggtttcaatc cacgcggtaa
    28021 gttttggcgg cgcaatcagc gatttatcgc ttaacaccag catcggcccg ttaccaatct
    28081 gacgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta tcaagcacaa
    28141 tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc gccgcgtaat cccacctctt
    28201 cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc agctcacgta
    28261 gtaacgtcac cagcagatag caactgaggc gatcatcaaa ggctttgccc atcacccgct
    28321 ggtgagggag gacctgaaaa gtggtatcga aggtgacgcg atcgcctgga cgaattcccg
    28381 cctgcatcac ttcgtcatag gtgcgcgcgc caatgtccac gcgcatggcg ctaacgtcat
    28441 tcccctgccg gtcgccgtca agcaggcctg gaattttgca ctcttcacgg gtggtgatgc
    28501 gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc acgtcaatag
    28561 cgccttcgcg ggagatgctg cgcaccataa atcccacttc gtccatatgg gcgcagatca
    28621 tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga ccatcaaatc
    28681 gcacttcttt ttgcaggcga gccgcttctt ccagcaggat ctgccgcact tcctgttccg
    28741 aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc attatgcctc
    28801 tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt gcggggtaac
    28861 cagcacaaca tcttcgatgc gcacgccccc ttgccctagc aaataaatcc ccggctccac
    28921 ggtcagcaac atgcctggct gtagcgtcgt ggtgtcccgc ggtgaaaaac gcggatcttc
    28981 atggacttca atgccgatag cgtgaccagt gttatgaccg aaatagtcgc caaatccagc
    29041 ctcggtaatg acccggcgcg cggcgtcgtc aatctgctgg cagcgcacgc cggggcgaat
    29101 tgcagagatt gccgcgagct gtgcctgcag gacaatctga tagacgtcaa acagcgggtg
    29161 agattcggcg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat cagcagtagc
    29221 cctgatacag cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc
    29281 tggctttgcc gtgcggcagc gccccgcgcc agccgctggc gacaatggtg tcaaaagagg
    29341 ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc
    29401 tcatccccgc ctgaataaag cggcgaatat gctccgctcc gcgatcggca atcccacagg
    29461 cgaggcggat tatctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg
    29521 cgctaaccag tttcgcattg agttcagact tccagcggtg cgcggtttcc caactcacct
    29581 gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttga ttgacgatag
    29641 tggtaagcgt gttcgttgcg tcaagcaaat gcagctggta gccttgcgca cgggcttcca
    29701 cctcaacgaa atagcgcgaa tctaccagaa tgtgcgcact ttcacggcta ataaccacat
    29761 agcctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca
    29821 ccgcatccag ttgttgcacc ttaagccagt tgcgcagcga agcgagtaat gtcatctgga
    29881 ttttccttct tacaggctat cgattaacaa tttgcctttg cggaacatca tcagacgcag
    29941 gaacaccacc atcagcgcag taatgattgc ccccagcgcg atccccgcca tataaacgcc
    30001 caagttagtg accagcggcc atgcccagat agcggattcc gggaaccatt gcactgcgcc
    30061 cagccagaca gctgcggttg agccgacaat cgcgccgacc atatatgacg gaatagcggt
    30121 gatggggctt tccagcgcaa acggaatcgc accctcacta atccccataa aggccaggaa
    30181 catcgcggtc ttaccctgcg gataaagttg agcgttgaac aggcgtttgc cggttaaacg
    30241 acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag cgatagaacg
    30301 agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac ctgcggcttt
    30361 gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg cgtacatcag
    30421 cgcgcccttc tcacctgcgg cagtcagcac agtacggata ccgccgttga tccagccacc
    30481 aaagggagtg atgacgtagt acatcgccag catgacaaaa atggcggaaa gaatcggtag
    30541 caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct ggttcatcca
    30601 tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg tggacggcac
    30661 cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg
    30721 ggtagacatc aacccgccga taaaaccagc cgggaacgcc agtttgccac cgatagagtt
    30781 cgccacaaat gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc cgaaggactg
    30841 cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc cgctattaag
    30901 agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa tcagctggct
    30961 gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat
    31021 cacatgctgc gggagttcac cccagaagct gcttttagag gcctgcggat tcttaactgc
    31081 cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac
    31141 aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat
    31201 gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc
    31261 gaaacgttcg ttatcttccg gggtaactgc cacggaatgg atgatgatgg tcgcttcggc
    31321 gatatcctgc gccgtcaggc gattctggat gccgtccgcg ccctgggttt caattttcac
    31381 ttcataaccg gcttctaccg ccgcttcttc cagcacctga gcggccataa aagtgtgagc
    31441 caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcgtt
    31501 cagtgatagt tgactagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa
    31561 taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg tcggccttgt
    31621 gaggggacac aaagaaaaat tgaattaact gtatgattta aaagattatc gggagagtta
    31681 cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca
    31741 ccggaaccga gaagacccgg atagtcatgg acgatgagat acaccggaat atcatggaca
    31801 tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccggaggc tttgaagaac
    31861 tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg
    31921 agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa tgcgcggcgg
    31981 caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt gagattttct
    32041 ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa
    32101 agcacgcgct ccgccgaaac atgaccaatt tccgcacgca atatttcgag gataatggcc
    32161 tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa gcttacccaa
    32221 cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc gtaaaccgca
    32281 ataggcttac cttcgaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc
    32341 atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg
    32401 agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag
    32461 tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct
    32521 tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta
    32581 gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc
    32641 acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat
    32701 tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct
    32761 gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt gcagcgatgc
    32821 ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca
    32881 tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc
    32941 cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca
    33001 acgactgccg ggaactctgg ggatagccca ggattcaccc ctctggatca tcggtgtatt
    33061 aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc
    33121 agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc tacctagact
    33181 tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat
    33241 catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac cgcgcgtcaa
    33301 ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc
    33361 tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg
    33421 ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta ccggattatt
    33481 tttccatccc catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat
    33541 tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg
    33601 cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat ccggtgctcg tgctgggtat
    33661 tggcggattt attctcggaa ttctgggggt tattggtgga ccagtttcgc tgtttaaagg
    33721 gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt
    33781 gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg
    33841 gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt
    33901 tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg tgctggtggt
    33961 aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca ataccacatt
    34021 gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat
    34081 gatgatggtc aatcgtccga agcaacagcc accccacgat aatgtttagc aaaaaatgct
    34141 ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc
    34201 gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt
    34261 tctggcggcc gccctgatgg catttacccc gcttgcagca aatgcaggtg aaatcaccct
    34321 actgccatcg atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg
    34381 tggtcactgg cgagatcgtg attactggca tcgcaattat gaatggcgca aaaaccgctg
    34441 gtggcgtcat gataatggct atcatcgtgg ctgggataag cgtaaagcat atgagcgtgg
    34501 ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatgggca
    34561 ccgccattaa acgcgtcctc caatggagca caatgcctga tgcgacgcta gcgcgtctta
    34621 tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac gccgcatccg
    34681 ccaatagtgc cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaacaa
    34741 ccagatattc agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt
    34801 gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag
    34861 cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc
    34921 taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac gcacccacag
    34981 cgggatatgg aagcgaatga atccctgcat caccacctgc cccgccagtg tccccaccac
    35041 cgttgaggac agtccggcag caaccagact taacccaaag accgttgccg cagcatggct
    35101 taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga
    35161 aaagtggaac gccgccgcag ctgtagccat catcaccaga ttgacaaaac cggcaatcgt
    35221 catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg
    35281 taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa
    35341 cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt
    35401 acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac
    35461 aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat
    35521 taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag
    35581 aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat
    35641 aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat cacgaatctg
    35701 ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat
    35761 cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc tagcacccgc
    35821 ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg
    35881 tcccattaat gcgagcctca tcttacgcgc cgcccgtccg ctgctactct caacgcgata
    35941 gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgctaaacga
    36001 gaatgattat caaattcatt taaatggatt gtggtgattt ctctgataga ccaagattat
    36061 gattacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca
    36121 taactttcag cttccataca caacatagca gaaatgtatg acagatcact atttttgaag
    36181 cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat
    36241 agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc
    36301 attttgtact ggcacttgcc gttgttgtga ttctcgcact gctggtaagc agcgaccgca
    36361 aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt
    36421 tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa
    36481 aactgctcgg atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag
    36541 gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg
    36601 gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt ttcctgctct
    36661 ccaaagtcaa cggtatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg
    36721 gtcagtcaga aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta
    36781 tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt ggtgcataca
    36841 tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt actgaacatg ttcagcacct
    36901 ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc cagtgaagaa aacatccaga
    36961 tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag
    37021 gtttcaaagt tgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg
    37081 cgctgaacgc actgtttgcc accgttactg gctggtttgg ctacagcatc tccttccagg
    37141 gcatcctggg ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag
    37201 cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga
    37261 tggatctgca gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc atctctgtgt
    37321 tcctagtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc
    37381 tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt tacggctcta
    37441 ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac catacataaa
    37501 aaagccgggg atcattcccc ggctttttta cgcctgttaa tcagctaatg gctgcgggcg
    37561 accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg cctgctgctg
    37621 ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat
    37681 tgatctcaca atcatcgcat ccagcgtatt cgtgacaata tctttcacaa agacgccatc
    37741 aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat atccggtgcc
    37801 aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct
    37861 gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg cctgcgggga
    37921 gatgtgataa cgcttaaaca gacgaataat ccgcccggca atattctttt gcagcagcgt
    37981 gagcggcatt aaattgactg aaaagcgcgg tccttttttg tcgcaagggt gtgttgccag
    38041 ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc
    38101 aataaggggc agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag
    38161 gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc
    38221 accctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc
    38281 ggaaaccatc tcctctctgc tatccagcgc cagcacatga tgatgtgcgc aggattgctc
    38341 cgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg
    38401 cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact
    38461 attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc tcagcactaa
    38521 cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca acggcagcag
    38581 cgtacggcaa attgagcgga tacagtgaac gcgcatcatt aagccgtaat gacgactcat
    38641 aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccgcctctt gctccggcgc
    38701 ttgttccaac gcacgaaagt tgggtaggag cgttaacgga tctgttagcg cctgcaaatg
    38761 ccagcggcga ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac
    38821 gctaaaggaa atcagcaccg cgagaataaa tgccagcgaa tattcggttt ccaccccttg
    38881 caaaaagttc tgattgtaat tcagaaggca aagcgttgaa acagcccagg tgagatttaa
    38941 aaacggatag cgaagattac cgaccccgag ggtaaagatg atgaagaaaa cgggtaccag
    39001 gtaaccggca ataaagtcgt tttcataagg tgtgcacaac agaagtagca gcacgcttaa
    39061 cgctgccagc cagcttaagg taaatgcgag tgagatagta gaaaagcatg ttgtaaatca
    39121 gcactgcggt gaaaaggctt agtaaatcaa cgaccgtgaa aatggcatcc gcatcgccga
    39181 aaaaggtgga tatctttagc gggaaatcaa agaagctccc cacaagatac atgctgcatt
    39241 tgatgccgat cggcgtcacc aaaccaagcc agaacagacg ttgccagaca tatcggctgg
    39301 tcaatccata acgccaacgc gtccccagct gccagcgtaa tattgcgcag gcacacaaca
    39361 cgacaaacgt ctggctgccg agcatgaccg cagtctgtaa aaaagagaga tgaaagttcc
    39421 acagattggt gcaaaacatc ccgcataaaa cgggaacgac accgcgccag ccaaagataa
    39481 acaagattga cagcattacg cacagtggca tccacgccag aaagatataa ctggaatgga
    39541 taatggcaag tggcgaaata aaacgggata gctgaataag taccacggtg agcgtaaacg
    39601 ctagtgtgaa tatcttgata ttttttatca ggttatgctc cacaaacatg agtacactac
    39661 tctcactgat cggggttttc aggcacacaa ataataagtt tattcgcatc gcgaagcaaa
    39721 caggggccag cctgttgccg atttttcatc caatgatgct ttcaccaggc ggcgctaacc
    39781 cggacgtaga ctgtattaca aaagcggcaa aaagcagaga caaaaaaccc ccgctttgca
    39841 gcgagggttg gaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    39901 tgcaagcgct ctcccagctg agctatagcc ccacgatgcg tttacgtacc aaatttgctg
    39961 ggtgcaaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa
    40021 gcgctctccc agctgagcta tagccccgtc acgtaaagct tgtcgagttg acgggcggca
    40081 tcatatgaat tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg
    40141 aaaaatcagg caaatgaaca attttggaaa accgctcgca ttcagtagtt attcatgtca
    40201 cggtttcctg taaagtggtg ttataaaatg aactactaat agacccacat acattcaggg
    40261 aattgttatg ttcaaggaga ggatgacacc agatgaactt gccagactga ccggctatag
    40321 ccgccagacc attaataaat gggtacgcaa ggaaggctgg acaacgtcac caaaacctgg
    40381 cgtccagggt ggcaaagccc gactggttca cgtcaatgaa caggttcgtg aatatattcg
    40441 caatgctgaa cgtccagaag gtcagggaga agcgcctgcc ctttctggtg atgcgccgct
    40501 tgaagtattg ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa aacagtttac
    40561 atccttgctt ctgcgggaag ggattatcgg attgttacaa cgcttaggga ttcgcgatag
    40621 caaataatat gaaaagatta cgcaataaaa tgaccaccga agaactggct gaatgcctcg
    40681 gtgtggctaa acaaactgtt aaccgttgga ccagagaaaa aggctggaaa acggaaaaat
    40741 ttcccggcgt gaaaggcggt cgtgccagac ttattctggt cgatacgcaa gtttgcgagt
    40801 ttattcagaa cacgccagcc ttccataaca caccaatgtt gatggaagct gaagagagaa
    40861 ttgctgaata cgctcctgga gcacgcgccc cggcgtatcg acaaatcatc aacgcgatag
    40921 ataatatgac agacattgag caggaaaaag tcgcgcaatt tttgtcacgt gaaggaattc
    40981 gcaatttcct cgcccgtctc gatattgacg aatcagcata aataaaaaac ggcaggatat
    41041 tatctcctgc cgtttatctt tttacacgct aattactgct gattttcgcg ttcagcaata
    41101 aaatccagcg ctttgttgat acgctcgata ctgcgggtct taccaattgc gtgaacggta
    41161 acatccagtg ctggagactg ccccgcaccg gttacggcga cacgcagcgg cataccaact
    41221 ttacccatac ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg aacgttttca
    41281 gcggtccagt cagtaatcgc ggccagtttg tcacgaacca cttccagcgg ctgacgcgct
    41341 accggacgca gatgtttttt cgccgcatcg gcatcgaact cagcaaaatc ttcgtagaaa
    41401 taacggcagc tctgtgccat ctctttcagc gtcttgcagc gttcgcccag cagtttcacc
    41461 agatcagcca gctgcgggcc gttacgggta tcgatatttt cctgctcaat gtgccactgt
    41521 aagtgagtag caacatactc cggcggcagc gcgttaatgt agtgatggtt cagccacagc
    41581 agcttgtcgg tgttgaacgc actggcagat ttgctgacgg cattcaaagt gaagtatttg
    41641 atcatctctt cacgagtgaa gatttcctga tcgccgtggg accagcccag acgcaccaga
    41701 tagttcagca gtgcttctgg caaataaccg tcatcacgat actgcattac gctgactgcc
    41761 ccgtgacgtt tggacagttt tttaccgtca tcgccattga tcatagaaac gtgcgcgtaa
    41821 accggcaccg gcgctttcag ggccttaaga atgttgatct ggcgcggcgt gttgttgata
    41881 tggtcttcgc cacggataac gtgggtgatt tccatatccc agtcatcgac aaccacacag
    41941 aagttatagg ttggggaacc atcggtacgg cggataataa gatcgtccag ttcctggttg
    42001 ctgaactcga tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc ctgcgggtta
    42061 gcaaaacgta caacacacgg ttcatcatca gcatgatgct catggctgtg gcggcagcga
    42121 ccgtcataac gcggcttctc acctttcgcc atttgctctt cgcgcagcgc ttccaggcgc
    42181 tctttagagc aatagcattt ataagcagtg ccctcttcca gcatctgatc gatcaccgcg
    42241 ttgtagcgat caaaacgttt ggtctggtag tacggacctt catcccactc caggctcagc
    42301 cagttcatgc catccataat ggcttcgata gcttccggcg tggaacgctc aagatcggtg
    42361 tcttcaatac gcagcacgaa ctcaccgccg tggttacgtg caaaaagcca ggagtaaaga
    42421 gcagtacgcg cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa gcgagttttg
    42481 attttcatga aatggcctta cgtttagaaa gatgccgaca accggcaaat cctggaaaaa
    42541 ttgagtgggc gatattctat cactcccgcc tgattcctca atgtagttcg ggtttctacc
    42601 ttacgcatta ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc gatcatcttg
    42661 cttaaattta cgacgaacga acaatttctt tagaaaatgc gttgactcat tttgaactct
    42721 ccctataatg cgactccaca cagcgggggt gattagctca gctgggagag cacctccctt
    42781 acaaggaggg ggtcggcggt tcgatcccgt catcacccac caactacttt atgtagtctc
    42841 cgccgtgtag caagaaattg agaagtgggt gattagctca gctgggagag cacctccctt
    42901 acaaggaggg ggtcggcggt tcgatcccgt catcacccac cacttcgggt cgttagctca
    42961 gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac
    43021 caatgtaaaa aagcgcccta aaggcgcttt tttgctatct gcgatactca aagattcgaa
    43081 cctgcagcag gtttgagttg agcgcagcga aacaacggag ccgctcgcgg cgacggcccg
    43141 aagggcgagc gaagcgagtc atcctgcacg acccaccaat gcaaaaaagc gccctaaagg
    43201 cgcttttttg ctatctgcga tttgtaagat tgatttttct tctcaagttc cttgttcttc
    43261 taccgatatt acctcgtaca gtgacgagga ctgaacaatg gccactatct cttcaacttc
    43321 aattccttcc atacaaacac agagtagtaa cagggcatca caaggtagcg atgttgcttc
    43381 acaaatcgcg cggataagtc agcaaatcat caaactcact caacaaatta aagagattgt
    43441 agacactagc ggcagtgctg aagacaagca aaaacaggcc gaattgattc aacagcaaat
    43501 caccctactg gaaacacaac tggcgcagtt gcagaaacag caggcagaaa aggcgcagga
    43561 gaaagagcag cgtttgtctc tgaatgtatc ccttctaaat cctgttgaaa aaacgactca
    43621 tatcgatatc tatatctgat acaacggatc cccttcccga ccagccatta gcgcattgcc
    43681 ctggtctcgt accacgtccc ataacgcttc agcggcggtg gaaagtgaac ggttcttcct
    43741 gcgcaccagc atcaactggc gttcaaccac cggcgtaatg cgcttaacca ccagtgggct
    43801 accttcaggc aatggtagcg ccagtgcagg aagaatactg atgccgatgc ctgcggctac
    43861 catcgggaac agcgtcgccg gatgtccaat ctcctgcaca atattcgcct gaataccatt
    43921 gcgcgccagc gctgcgtcaa tcagcggtcg gctgcctgac gcgtaatcct gcaacaccaa
    43981 tttcgctccc tgtaatgcct gccagggtac ataatcttcc acggccaaag cgctatcgcg
    44041 atggcaaagc agaaagaaag gctcggaaag aatcgcttca cattgcagat cgcccactgg
    44101 gcctggatcg atgacgatgc caaagtcgac atctccctgg cgaatacttt ccatcaccca
    44161 ctgctgcgga cgatcatgca ggacaaactg aatatctgga tagcggcgat ggctttcagc
    44221 aatgcattgc ggaataagat gcgcggaaat ggtctggctg gcagcgaccc gaacttttcc
    44281 gctcagttgt tgccccatac gcccggtatc gcgcaacgtg ctgttaagtt catccagcag
    44341 tcgctcaaga cgcaaagcca actgctgccc tgcatctgta agcaccactt cacgcgtggt
    44401 tctgtccagc aggcgaacac cagtatgatt ttccagctcc ttcacactgt gacttactgc
    44461 cgactggctc aagccgatac gctctcctgc acgactaaaa cttttctcct gcgctactgt
    44521 gacgaaaacc tttaattgtt ttaaagaata attcatctgt tttcttcatg aatggatgca
    44581 ataaatcaat tttatttctc aaactggaag aagcacaata gaaccatcga tcatctggag
    44641 tctttatgaa actttttcgt atcctcgatc ctttcacctt aaccctgatc acggtggtgt
    44701 tgctggcctc tttctttccg gccagaggcg atttcgtccc cttctttgaa aatctgacca
    44761 ccgcagctat tgccctgctg ttctttatgc acggcgcgaa gttgtcgcgt gaggcgatta
    44821 ttgctggcgg tggtcactgg cgactgcatt tgtgggtaat gtgcagcacc ttcgtgctgt
    44881 ttccgattct gggtgtactg tttgcctggt ggaaaccggt aaatgtcgac ccgatgctct
    44941 actccggttt tctctacttg tgcattctcc cggctaccgt gcagtctgca atcgccttca
    45001 cgtcaatggc gggcggtaac gtcgcggcgg cggtttgttc tgcgtcagca tccagcctgc
    45061 tggggatttt cctttcacca ttgctggttg gtctggtgat gaatgttcac ggtgcaaggg
    45121 gcagccttga gcaggtcggt aaaattatgc tgcaactgct gctgccgttt gtgttggggc
    45181 atctttcccg gccgtggatt ggtgactggg tgtcgcgcaa taaaaaatgg attgcgaaaa
    45241 ctgaccagac gtccattctg ttggtggttt atacagcgtt cagcgaagcc gtcgttaatg
    45301 gtatctggca taaagttggc tggggatcat tgctgtttat cgtggtggtc agctgcgttc
    45361 ttctggctat cgtgattgta gttaacgtct ttatggcacg ccgactgagc ttcaataagg
    45421 cagatgaaat tactatcgtc ttttgtggtt cgaaaaagag tctggcaaat ggcatcccga
    45481 tggcaaacat tctgttcccc acatcggtga tcggtatgat ggtgctgccc ctgatgattt
    45541 tccatcagat ccaattgatg gtctgtgcgg tgctggcgcg tcgatacaaa cgccagaccg
    45601 aacagttaca ggcgcagcag gaaagcagcg ccgataaagc ttaaagcgga cgcttcaggg
    45661 gctggaccag ttgcgtcagc ccctcggttt taatcagcag cgtgatttgc attagctcac
    45721 cgagcttccc tgccgggaac tcatctttgc gggcaaacca cagcagatac tcctccggca
    45781 ggtcaattaa gcgacgccct ttgtatttgc caaacggcat tatcgtattg gctatttcaa
    45841 tcagctgctc tttttccatc tcagctaccc agcaaacgca gcatttccgc ttcgtcgatg
    45901 acttcaatgc ccagttcctg cgccttcgcc agtttagatc ctgcagcttc acccgctatc
    45961 accagatcgg ttttcttcga cacgctgccc gcgactttcg cgcccagttc gaccagtcga
    46021 gctttagcgt catcacgcga catctggctt aagctgcccg taagcaccac ggttttacca
    46081 gcaaacgggc tgtcaatctc ttccgcgttg ataacgatcg gcgcaggcca gtgaacacct
    46141 tccgccaaca gctcgctgat gacattgcgg ttgctttctt cggcaaagaa gttgtgaacg
    46201 tgggatgcaa cgacaatgcc aacatcaggc accttttgca gctcttcaat cgaagcggct
    46261 tccagcgctt ccagcgtgcc gaaatatgcc gccagacctg ctgcggtggc ctcgccgact
    46321 tcacggatgc caagtgcata gaggaagcga gcaaaggtgg tttctttcgc tttttccagc
    46381 gcgttaacca cgttttgtgc caattttggc cccatacgct ccagtccggt cagtttgcct
    46441 gcggtgagtt tgaacagatc tgccggagta tggacatatt ctttttcaac cagctgatcg
    46501 atgattttgt cgcccattcc gtcaacatcc atcgcacggc gggaaacaaa gtgtttcagc
    46561 gactctttac gctgcgcacc gcaaatcagg ccaccggtac agcgggcaac cgcttcacct
    46621 tccacacgct caacgtcaga accacatacc ggacaatgcg tcgggaatac aacctcacgg
    46681 gtatcttccg ggcgttcaga aagcacgacg ttaaccacct gcgggatcac gtcgccagcg
    46741 cggcgaatca ccactttatc gccaatgcgt aaaccaagac gttcgatttc atccgcattg
    46801 tgtaaggttg cgttactcac cagcacgcca gcaacatgga caggttccag acgcgcaaca
    46861 ggcgtaatcg cgccagtacg cccaacctga aactcgacgt cacgcacaaa ggtcatctgc
    46921 tcctgcgccg ggaatttaaa cgcaaccgcc cagcgcgggg cacgcgcgac aaagccaagc
    46981 tgctcctgct gttccagtga gttgacctta atcaccacgc cgtcgatatc aaagcccagc
    47041 gtcgggcggt cttcttccac tttgtgatag aacgccagca cttcttccgc cgattcacaa
    47101 agcgttaccc gatcgctgac cggcaccccc cactttttaa attgcagtaa acggccaaga
    47161 tgagtatccg gcagctcgcc accttccaga acaccaacgc catagcagaa aaaagtgagc
    47221 ggtcgcttcg ctgtaatacg cggatcaagc tgacgcagtg aaccagctgc cgcattacgt
    47281 gggttagcaa aaactttccc gcccgtgcgt cgcgcatctt cgttaatctt ttcgaacccc
    47341 gcctgcggca ggaacacttc accacgcact tccagacgcg ccgggatatt ctctccgtgc
    47401 agcttcagcg gaatggcgcg aatagtacgc acattagacg tgatatcttc cccggtggtg
    47461 ccatcgccac gggtcgcggc actgactaaa acgccatttt catacagaat actgacggca
    47521 aggccatcca gcttcagctc acagcaccag gtgactttct cgttgttttt cagacggtcc
    47581 tgcacacgtt tgttgaaagc aagaaagctt tcttcatcaa aaacgttatc cagtgacagc
    47641 attggtactt catggcgtat ctggctgaaa gctgccagcg gcgcagcacc tacacgttgg
    47701 gtaggcgaat caggcgtaat cagttctgga tgtttggttt ccagctcgcg cagttcgcgc
    47761 atcagcctgt cgtattcagc gtcgggaatt tccggcgcat ccatcacatg ataaagatat
    47821 tcatgatggc aaagcgtcgt tcgcagttct gtcagttgtt gttcgattga ttccatatcg
    47881 caccatcaat gctaaaaacc cccgacaagc gggggttcga agaggagtta atttgcctta
    47941 agtgtatcag gcgttggcgt ctttgacttc gcggatgatg tcctggtact cgcgcaattt
    48001 ctgcggagtc atcatacggc gctgatcgtc aagcacgaca ccgcccactt catcggcaat
    48061 atgctgcgca gattgcagca tcagcttgaa gttctgcagc tcgtcaccgt aagacggtac
    48121 ctgcataaag atagtgacac ccggagtagt gaaatccttc atttcaggat caaaggttcc
    48181 cggtttcacc atattcgcca ggctgaataa cgccgggccg ctgccatccg ggctaagatg
    48241 acgatggtaa atattcatat cgccaaaaat gaagcccgct tgttgaatgc tgttaagaag
    48301 cagttcaccg tttagctcgc taccgtgatg cgccgcgacg ttcatgataa tcaccgcttc
    48361 tttgcgcttc ggtttatcca taactggagc cggttccgct acaggctcag gctgtggtgc
    48421 cgctacgggt tctgcaggct ggaaagcctg ttgtgccggt tgcggtgctg aatgcacagg
    48481 ctgcggcgct ggcgcgacct gtggcgaaac tggctgctgc aacggctgtt caggctgcgg
    48541 ctgataggca ggctgctgca ccggctgcgc tggacgcgga gcatgttgcg gcggtacctg
    48601 cgcttcaggc ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggtaggta
    48661 ctggtgttgc ggcgacggac gagcagcctc atgctcctga gcgttagccg gggcatgatt
    48721 cacgcggtga acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga
    48781 atcgtcgtca cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg
    48841 ttctttacgg ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac
    48901 aatgattaat atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg
    48961 ccaccacggc aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca
    49021 ctattaactt tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca
    49081 tatcctaact gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg
    49141 tcaaaaggag ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt
    49201 ttactatttt gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat
    49261 tttaccgctg ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacaca
    49321 gctcgatgtc tggatcccga ctttcatgag ttacgttccg gactggctac aatggctgag
    49381 ttatctgttg tggcctctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc
    49441 cacgattgct aactggattg ccgctccgtt taacggttta ttggctgaac aactggaagc
    49501 acgattgact ggcgctacac cgccagatac cgggattttc ggtatcatga aagatgtgcc
    49561 gcgaatcatg aaacgcgaat ggcaaaaatt tgtctggtat ctgccgcgcg caattgtatt
    49621 actaattctt tacttcatcc ctggtattgg gcaaaccgtc gcgccggtac tgtggttcct
    49681 gtttagcgcc tggatgttag ccatccagta ttgcgattac cccttcgata accacaaagt
    49741 gccgtttaaa gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg
    49801 tgctttaacc agcctgttta cgatgatccc gctgcttaat ctgttcatca tgcccgttgc
    49861 cgtttgtggc gcgacggcga tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg
    49921 gtaacaatct accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc
    49981 cccttattcc atcttgcatg tcattatttc ccttctgtat atagatatgc taaatcctta
    50041 cttccgcata ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag
    50101 gacaggccat gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg
    50161 ttcgcctgaa tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca
    50221 gcttcagcgt taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg
    50281 tgctgaaacc aggcgttgaa ctggttgaac cgaccagcgg taataccggg attgcactgg
    50341 cctatgtagc tgccgctcgc ggttacaaac tcaccctgac catgccagaa accatgagta
    50401 ttgaacgccg caagctgctg aaagcgttag gtgcaaacct ggtgctgacg gaaggtgcta
    50461 aaggcatgaa aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat
    50521 acctgctgct gcaacaattc agcaatccgg caaaccctga aattcacgaa aaaaccaccg
    50581 gtccggagat ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga
    50641 ctggcggtac gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc
    50701 ttatctctgt cgccgttgag ccaaccgatt ctccagttat cgcccaggtg ctggcaggtg
    50761 aagagattaa acctggcccg cataaaattc agggtattgg cgctggtttt atcccggcta
    50821 acctcgatct caagctggtc gataaagtca ttggcatcac caatgaagaa gccatttcta
    50881 ccgcccgtcg tctaatggaa gaagaaggta ttcttgcagg tatctcttct ggagcagctg
    50941 ttgccgcagc gttgaaacta caagaagatg aaagctttac caacaagaat attgtggtta
    51001 ttctaccatc atcgggtgag cgttatttaa gcaccgcatt gtttgccgat ctcttcactg
    51061 agaaagaatt gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc acctttttag
    51121 gtgctttttt gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg
    51181 aactggtatt taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc
    51241 caaagctgaa tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat
    51301 gacgtacgaa acgtcagcgg tcaacacccg ccagcaatgg actgtattgc gctcttcgtg
    51361 cgtcgcgtct gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag
    51421 actttagttc cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt
    51481 taccattacc gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc
    51541 taagggcttc acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag
    51601 cctgtttaaa ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga
    51661 aggcgaagac gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta
    51721 atttcccggg ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt
    51781 ttagcatccc cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc
    51841 attgaccgga aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc
    51901 ggtcgtgcca aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc
    51961 ggtgaagaaa aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg
    52021 gagcaggaaa tcatagccct gattaaagat aaacacatga cagctgacgc agctgctcat
    52081 gaagttatcg aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa
    52141 cgtgcggctg acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag
    52201 attatcgacc tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacgccg
    52261 tccgaaaccg cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc
    52321 cgtacttccc acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc
    52381 ggtagcgtca cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat
    52441 caggtttacg tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa
    52501 gtggcttctg aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac
    52561 ggtcaccagg tagaagtatg cgctaacatt ggtacggttc gtgacgttga aggtgcagag
    52621 cgtaacggcg ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac
    52681 gcgctgccca ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc
    52741 tcgcaggcgg ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg
    52801 aacttcccga aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggat
    52861 cgtaaagaga tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa
    52921 ttgcgcatta tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag
    52981 atcgaaatct acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa
    53041 atcggcgtaa tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa
    53101 gttgatttct ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt
    53161 ggtaatgata tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc
    53221 aagcaagtta ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt
    53281 gctggcgatg aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg
    53341 agcgccattt ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg
    53401 aaggtgttag cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt
    53461 aacaagttca ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc
    53521 ttaggagaag atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaaaaa
    53581 ggataccgga actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga
    53641 cgtgccggat gtcgtttttg cggaaaaaat cgttggtgat ggtattgcta tcaaaccaac
    53701 gggtaacaaa atggtcgcgc ctgtagacgg taccattggt aaaatctttg aaaccaatca
    53761 cgcgttctct atcgaatctg atagcggcgt tgaactgttc gtccacttcg gtatcgacac
    53821 cgttgaactg aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt
    53881 tggcgatact gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct
    53941 gactccggtt gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag
    54001 cgtaaccgtg ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa
    54061 aatggcgccc atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc
    54121 gctctcatgc tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg cacggtgcac
    54181 cgcatcgttt aacgccttcc ctttcagcaa accactgata agctgagcac aaaacaggtc
    54241 gccagtccct ttcaggtcgg tttttacccg tgaatgggaa atgacattca cgctgtcggc
    54301 actgaccacc accacctgca tctcctgatt ttcttcatta ccggaggcgc tggtaatcac
    54361 cacccatttt aatgtgtctg aaagcagact ttttgcggca gcaatggcac tgtcgagatc
    54421 gcggcaattt ttaccggtca ggatttccag ctcaaagata ttgggggtaa ttccctgcgc
    54481 cagcggcagt aaatattgtc gatacgcctc gggaaggtca ggtttgacat aaattccgct
    54541 atcaatatcg ccaatcaccg gatcgaccat gatcaatagg tcaggatggt ctttgcgtag
    54601 cgcagtcagc cactcggcaa ggattttgat ttgcgatgcc gttcccatat agcccgtggt
    54661 tacagcacga agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa
    54721 ccattcgtcc ggaatcgcac caccgtagaa agtgtcataa tgcggcgtat tgctcagcaa
    54781 taccgtcggc acggcaaaga cattcaagcc gttctgtttt atagcaggca ccgcaatgct
    54841 gttgcctacg cttccgtaca ctacctgcga ctgcacggcg acgatatccg cctgcagcgc
    54901 cctactctta tcgttaaaca acaacaaact actcatttaa ttttttctcc ttgccgatga
    54961 tcctcatcgt attccaaccg aaactttacc tgattctggc agtcaaatcg gctattacaa
    55021 aacaaagata aggtaattca gtgaagaaaa tcatttgtct ggccattacg ctgctaatga
    55081 cactccccgc ttacgcgaag ttaactgttc atgaagaagc ccgcatcaac gccatgctgg
    55141 agggattagc acagaaaaag gatttgatat ttgtgcgcaa cggtgatgaa catacctgcg
    55201 atgaagcggt ttcccacctg cgtctgaagc tcggcaatac ccgtaaccgc attgacactg
    55261 ccgagcagtt tattgataag gtagcttcgt cgtcatcgat taccgggaag ccgtatatcg
    55321 tgaagatccc cggtaagagc gatgagaacg cacagccttt tttacatgcg ttaattgcgc
    55381 agacggataa aacggtgcct gcggaaggaa attaatccgc tttggtaagg catttacagg
    55441 aggtaacatg aaaaaatgct ttatttatca cgatgaaaaa tcgaataaat tttggtggat
    55501 agattacgaa ggggatagtt tagctgtcaa ctatggcaag gtaggtagta ttggtaaatt
    55561 ccatacaaaa gaattcgata atgaagaaca gtgtctgaaa gaagccagta aattgattgc
    55621 cgcaaaaatg aaaaaaggat atcaagaaac tccagacttt aacttcatgg atctctacta
    55681 ttttgatgat gaagagattg ggttacatct taaaacgtca cacccaaact tccagtgcca
    55741 ttttactgat ccactttata tgtgttgctg ggatgaagaa tctccttttg gtagcgatga
    55801 aggtgctgat gctctaaacg aacttgaaaa tagcctccgt tctggactgt gctgatttcc
    55861 ctcaaatgtt aattgaaact atgtggggta tgaaatacat cgctatggac agtattcttg
    55921 aagaggatgt gcgtgcgcaa ttactagccg atgaaatgag ctctatccag agcaatatga
    55981 ttacctacgc gactgcattc ggtcagatca aagtcatggg taaaatctcc cataaactta
    56041 aaaagatggg actcaatgca ctagcgcgtc atcagcttac cgcaaaaatt cttcaatggg
    56101 gtgacggtca ggactcacca atacttcaaa aaatgattga tgaccttact gcttttccac
    56161 acgaaaatta aaaactgcat ttgtcggcag caacaactgc cgacaaatca gattattgac
    56221 aaagtgcact ttgtttatgc cggatgcggc gtaaacgcct gatccggcat aaaaatattg
    56281 ctaattcaat atattgcaga aacattgtag gcctgataag cggagcgcat caggcagttt
    56341 tgcgtttgtc atcagtctcc aatgctatta atccttaaat ccctgccccc tgactaaaat
    56401 gctcttcccc aaacactccg gtggagaggt agcgatcgcc acgatcgcag atgatcgcta
    56461 ccaccaccgc accagggtta gcttttgcca cccgcagtgc tccagcaacc gcgccgcctg
    56521 agctgacgcc acagaatatt ccttcccgca ccgccagttc gcgcatggtg ttttccgcat
    56581 cgcgctgatg aatatccagc acctcatcca ccagcgcagc gttgaaaatc cccggcagat
    56641 attccgcagg ccagcggcga atgcccggaa tactgctgcc ttcttccggt tgcaggccaa
    56701 caatggtcac cggtttggat tgttcgcgca taaagcatga aacgccggtg atagtgccgg
    56761 tcgtccccat gctggagaca aaatgagtga tgcgcccgcc ggtttgctgc cagatttccg
    56821 gcccggtggt ggtgtaatgc gcataagggt tatcgggatt attgaactga tcgagcagct
    56881 ttccttcgcc acgattcgcc atctccagcg ccagatcgcg cgcaccttcc atgccctgct
    56941 ctttggtgac aagaatcagt tccgcaccat aagcacgcat cgccgcacgg cgttcctggc
    57001 tcatgttgtc gggcatcagc aatttcatgc gatagccttt cagcgcggca atcattgcca
    57061 gcgcaatgcc ggtgttacca ctggtggctt cgattaagac atcacccggt ttaatttccc
    57121 cgcgcttttc cgcctcgacg atcatcgaaa gtgccgcacg atctttcacc gaacctgccg
    57181 ggttattgcc ttccagtttt aaccacactt cactgccgtt atccggcccc attcgctgca
    57241 acttcaccag aggcgtattg cctattgttt gttctaatgt actcacaatc tctatccata
    57301 cgtggtgttg cctgatgcga cgcttgcgcg tcttatcagg cctacaggtt acaaaccttg
    57361 ccattaaaaa gcccggatcg cgggaagcgc ctccgggcgt ttaacattca ctcaacctat
    57421 caggcgcttt gtgcgagagc aagttcctca tcgcgggttt caatacgctc gtcgccgtta
    57481 tacagccgcg catgttgcag accaacgaat aaacgctcgc catgctgcgg ggcatcgtcg
    57541 ccatgcatca cgaccgtcag cggttcgttg taccacccca gcggctgcac cactaattgg
    57601 gtgtagtgac ctttcgggct ggcttccagt acctgtaccg gcagcggcga atcgagactg
    57661 gtacggcggc tgatatccac ttcccacggg cgcaggaaga gatccaccgg cccctgatac
    57721 gcaggtgtgt aacccagcgg ccagcgatgc gcgccaacat ggaactgccc gccgcgaatg
    57781 gttccctgca ggcggttcac ttcgcccata aattcgagta caaaacgggt cgctggttcg
    57841 cgccatacct gattcggcgc atcagcctgt tcaatattcc cctgactcat caccactaca
    57901 cgatcggcta cttcggtcgc ctcttcctgg tcgtgggtca caaacacgct ggtgaatttc
    57961 agctcttcat gcagttgacg cagccagcga cgcagctctt tacgcacctg cgcatccagc
    58021 gcgccgaacg gttcatcaag cagcagaatt tgcggttcta cagccagcgc gcgcgccagc
    58081 gccacacgct gtttctggcc gccggaaagc tgcgccggat aacgatccgc cagatgcgca
    58141 agctggacca tttccagcaa ttttgtcact ttcgctttga tggctgcggc attcgggcgc
    58201 tcgcgacgcg gcagcaccgt caggccaaaa gtgatattgt cgaataccgt catatggcgg
    58261 aacagcgcgt aatgctggaa cacgaaaccg actttacgat cgcgcgcgtg caggcgactc
    58321 acgtcggtgc cgtggaagcg aatatgcccg ctggtttgat gctccagccc ggcgataatc
    58381 cgcagcagcg tggttttccc ggaaccggac ggccccagca acgcgaccat ctgaccagaa
    58441 ggaatatcca gtgagatatc gttcagcacc tgggtgcgac caaaagactt cttgatattg
    58501 gcaatctcaa tgctcatgat gctcctcctg ctgcgcgcgt ttttcctgat tctccaggcg
    58561 ccactgcaac atactcttta aaaacagggt gataatcgcc atcaacgtca acagcgcggc
    58621 ggcggtaaag gagccgacgg tgttgtagtc ctgctccaac aattcaatct gtaacggcag
    58681 cgaaagtgtt tcgccgcgaa tcgagccgga aaccaccgac actgcaccaa actcgccaat
    58741 cgcgcgggcg ttggtcagca ccacgccgta aagcagcgcc cagcgaatat tcggcaatgt
    58801 gacgcgacgg aacatctgcc agccggacgc gccaagcaaa atcgccgctt cgtcttcctg
    58861 gctgccctgg cttaacatca ccggcaccag ctcgcgcacc acaaacggac acgtcacaaa
    58921 gatggtgacc agcaccattc cgggccagga gaacataatt tgcagattat gttcatccag
    58981 ccagccgccg agcgggccgt tggagccgta gaacagcaga tacaccagac cggcaaccac
    59041 cggcgataca gcaaacggaa tgtccagtag cgtcagcagt aactggcgtc ccgggaagtt
    59101 aaagcgcgtc accagccagg ccagcagaat gccgaacacc aggtttaccg gtacggcaat
    59161 cagcgcgatc atcaccgtca gccagatggc gtgcagcatg tccggatcgg ccagattctg
    59221 taaaaccggc atcagcccct tgctgaatgc ctgcacgaag atgtaaatca tcggcaccag
    59281 caggatgaac gccgaaacca gcatcccgat gccaatcaga aaccatttgc cccagttaat
    59341 cgggcgcgcg tcataacgct tcaattgggt aacttccgcc attaatgacc taccacacgc
    59401 cgaccaaagc gactttgcag agtgttaatt gagaacagca gcagcagaga tgccgcgagg
    59461 atcaccgaag caatcgcgct cgctgccggg taatcaaact cctgtaagcg cacaaaaatc
    59521 atcagcgacg tcacttccgt cttccacgcg atatttccgg cgataaaaat caccgcgcca
    59581 aattcaccaa gactacgggt aaacgacagc gccacgcccg ccaccagcgc cggagaaagc
    59641 tccggcagca ccactttgca gaaactctgc cagcgcgttg caccaagcgt ttccgccgct
    59701 tcttcatatt ccgggcctaa ctcttccagc accggctgca cggtacgcac cacaaacgga
    59761 atgctggtaa aggccatcgc caccgcaata ccaagccagg tataggtgac tttgatatca
    59821 aacttcgcca gccattcacc gtaaaaaccg tttacggaaa agagcgaggc cagcgttaaa
    59881 ccggcgactg ccgttggcag cgcaaagggt aaatccatca gcgcatcaag cagcgtgcgg
    59941 cccggaaagc gataacgtgt taaaatccac gccatcagca gaccgaaaac gccgttaaaa
    60001 atcgatgcca caaacgccga cagcagcgtc actttgtagg ccgcgaccac ctgcgggttg
    60061 gtgatcacct cccagtactg cgcccagctc atctcagaaa gctgcatcac cagcgcagag
    60121 agcggcagca gcaatatcag gcacacaaac agcagactgg tgccaaggct taaagtaaag
    60181 cccggcagca cgcgtcttga agaaactgca aacattactt acgccccgcc gctaacagct
    60241 tgtctaactc gccgccgctg gtgaagtggg ttttcatcac ttccggccat gagccaaatt
    60301 tgtcttccac gcggaacagt tcggtctgcg ggaatttatc tttcagtttg tccatgactt
    60361 ccgggttatt cacacggtag taatagtcgg tgatgatggt ctgcgcctgt gggctgtaga
    60421 gccagttcag gtaggctttt gcggcttttt ccgtaccgtt ggcctgcaca tttttatcga
    60481 cccacgccac cgggaattcc gccagaatat tggttttcgg gatcaccact tcaaagccct
    60541 gtgcttcata ctgtttacgg atgttgttca cttccgattc aaagctaatc agcacatcgc
    60601 ccaagccacg ctcggcaaaa gtggtggttg cgccacgacc gccggtatcg aacacttcaa
    60661 cgtttttcag gaactgggtc ataaactgtt cggttttggc tttatcgcca ccgtcagctt
    60721 tatccgctgc gccccatgcc gccagatagg tataacgcgc gttacccgac gttttcgggt
    60781 tcgggaaaat cagcttcacg tcggagcgca ccaggtcgtt ccagtcgtgg atattcttcg
    60841 ggttgccctt acgcaccagg aagcccatgg tggagtagaa cggcgagcta ttattcggca
    60901 ggcgcgactg ccagtcagcc gggatcagct tgcctttgtc gtgcaggatt tgtacgtcgg
    60961 tcacctggtt ataagtgaca acgtctgctt ttaagccctg caaaatcgcc agcgcctgtt
    61021 ttgatgaccc ggcatgagat tgttttatcg tcagtttgtc gccgccgtta tcttttgccc
    61081 attgctgttc aaacggcgga ttcagggcgg caaacagctc gcgggagacg tcataagaac
    61141 tgttcagcag ttccgttgcc tgtacatggc ccgccagcag cagagaagcg accagcgcga
    61201 gtgagttctt tttcagtaag ttaacggcca ttgcgcaccc ttataaattt aatgactttc
    61261 taatagccat catatttata acggacgtga aaggagtaac ggttttatat accgtttggt
    61321 gatttggaag ttaaaaaggg aataagaact tggttcaaca ggcgtaattg ttgcaggcag
    61381 tttggacacg gacagcgcgg agaaaccgga gcgtacacat agtacgtgag gatttcgagc
    61441 actgcccggg gccaaaatga caaatgaaat agcctggtga acttagttca agacgaaatc
    61501 ctccccacac atgcagggag gaaacagagg tgaatcagat accgacgcta accgtctccg
    61561 gcagtgtgct gccgccatca atcacattct gtgtaccggt taaatagctg gattcatccg
    61621 atgcgaggaa ggccgccagt tcgccgactt ccagcggatc ggcgaggcga cacagcggga
    61681 ttgcttttgc catttcagtc agcaccgatt ctggatcttc cgggttcgac tggcgggcaa
    61741 tgctttccgc cattggcgtg cggacgtatc ccgggcagat ggcgttaacg cgaataccgg
    61801 attgcgcgta ctccaccgcc agcgatttag tcaggccaac aatcgccgct ttcgttaagg
    61861 catacgccgt ttcgccagga tcggccacca tatcaccagt gactgaagac atcatcacaa
    61921 tgcgaccatc tttgcgcgca atcatctccg gcagcaccgc cttcgtgacg ttccatacgc
    61981 ctttaatatt gatatcaata tggaaatcgc gatcttcatc gctcatatcg aggaaactgc
    62041 ccagacgaca aacgcctgcg ttattcacca ggatatcaat gcgcccttct ttttccttcg
    62101 cgcgtttgat agctgcggct accgacgccg ggtcacgcac atcggcgaca accgccgtac
    62161 agcgatgacc acgaccacac agttcgtccg ctagcttttc gatctcaggg gagatatcca
    62221 gcaagattag gttcgcgcca tgacgtgcaa aagttctggc aattccttcg ccaattccct
    62281 gcaatgcgcc cgtaatcagt gctgtcttgc ccgtgagttt acccatttta atgctctcct
    62341 tgttaataca gagcgttaca ctccattccc tctgaaaata caggataaac aaaatattgc
    62401 tgcggatcag ctcactaatt caggaaaatc tcaatccagt cgctgcttta tgcatcattt
    62461 caggcgttgt gttagctcac tgctgcgctg aatcattttc aatgacgcca catcattgat
    62521 gttgcacaag accaacaaac agtaaatcgg taacagaatt ttgcgccgtg cgggtagaca
    62581 tcgacgagct acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa tgcgccagcc
    62641 gccgcagcgg agagtcagcc agagaagtaa tggcaatcac cgttgccccc tgctttcgtg
    62701 ccgcttctgc gcacaagaca atctcttttt tactgccgct gtaagagata gcgatttgta
    62761 catcgccttt tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtcg gcttcgcagg
    62821 ccacgcgata gccaattttc atcagtttga acgacagatc gcgccccacc agcgccgatc
    62881 cgcccaggcc cgttatctgg ataaacggtg ctttactgat gacctcaatg attttttgca
    62941 gccgcgcgta gtcgaacaac gcacaggttt gctccagcgc cagctctttt tcgcgattca
    63001 gtttgcgggc aatcacttcc agcgaatcat cactggtaat cgaactgtgc aggtgcagcg
    63061 ccgtggcatt cgttttctcc cggctggcac tgtattcgcc aattaacgcc atacgcaatt
    63121 cagtaaatcc ttgcgcgccg agcttctggg caaatttcac aatgctcgac tggctgatac
    63181 cgagctgttt cgccatctgc cgtgaagaga cggatttcag ctcgctcact ctggcccgca
    63241 gaaagtcagc aattttttgc tcgttcccgg taaattcact ttccgcattg cgtattttcg
    63301 tcaggtacaa catcttccac tctcctgggc tggcagcaaa aattccgctt aagttcttat
    63361 gacgctcttc acactctgcg agtgtagact caatgattcc tttaagtctg tcaattcggg
    63421 atatatgatt ccatacatct gcaattattt gatcgtaaat agtaaggtca ccaccgatgc
    63481 aactagaaaa gatgattact gaaggctcga acaccgcctc ggctgaaatt gaccgcgtat
    63541 cgacgctgga aatgtgccgg attatcaacg atgaagataa aaccgtaccg cctgccgttg
    63601 atcgcgtcct gccggatatc gccgcggcga tcgatgttat ccacgcccag gtcagcggcg
    63661 gcggtcgtct gatttatctc ggtgcgggaa catccggtcg tctggggatt ctggatgcca
    63721 gcgaatgtcc gcccacctac ggcgtgaaac cgggtctggt ggttggtttg attgctggag
    63781 gcgaatatgc cattcagcac gcggtggaag gagctgaaga tagccgggaa ggtggtgtta
    63841 acgatctgaa aaatattaat ttaacggcac aggatgtggt ggttggcatt gctgccagcg
    63901 gtcgcacgcc gtatgtgatt gccggactgg aatacgcacg ccagctcggc tgccgcacag
    63961 tgggaatttc ctgtaatccg gggagcgccg tttcaaccac cgctgagttt gccattacac
    64021 cgattgtagg tgccgaagtt gttaccggtt cttcgcggat gaaagcaggt acagcgcaga
    64081 aactggtgct caatatgctt tccaccgggc tgatgattaa atccggcaaa gtgttcggca
    64141 acctgatggt cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag gtcaatatcg
    64201 ttaaaaacgc caccggatgt aatgccgaac aagcggaagc ggcgttaatt gcctgcgagc
    64261 gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct cgatgccgca gaagctaaaa
    64321 aacgcctgga tcaacacggc ggctttattc gtcaggtttt agacaaggaa taacccatgg
    64381 ccaaagagat cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc ggaccgggaa
    64441 atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat gacagttcac
    64501 tggttgatcc caatatcaaa acgctggaag gcgtgaaggg cgtcattttg accagcgacc
    64561 aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg atgagcgagc
    64621 tgctgggcga tgcaccggta caggatgccg cagaaatcgc cgcccagaac aaacgtcagt
    64681 taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc accatcttca
    64741 cgccgctgat ccccggtttt attgccgccg gtctgctgct ggggatagcg acgttaattg
    64801 ccacggtgat gcacgttccg gcagatgctc agggcacgct acccgacgcg ctgaatttta
    64861 tgaaggtgtt cagcaaaggt ttgttcacct tcctagtgat tctggtgggc tataacgccg
    64921 cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc tttttgctcg
    64981 gttataaccc cgctgcaacc accggttact acgccggttt tcacgatttc tttggtctgc
    65041 ccatcgatcc gcgcggcaat attatcggcg tgctgattgc cgcgtgggcc tgcgcccgca
    65101 ttgaaggcat ggtgcgccgc tttatgccgg acgatctcga catgctgctg acctcgttaa
    65161 tcaccctgct gatcaccgct acgcttgcgt acctgatcat tatgccgctg ggtggctggt
    65221 tattcgaagg tatgtcatgg ctgtttatgc acctgaacag taatccgttc ggttgtgcgg
    65281 ttttagccgg gctgttcctg atcgccgtgg tatttggcgt gcatcagggc tttattcctg
    65341 tttacctcgc gttaatggac agccagggat tcaacagctt atttcccatc ctttcaatgg
    65401 caggcgcggg ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa ccgcacagtg
    65461 cgctacgcag tcaggtacgc ggggcgatta ttcccggcct gctgggcgtt ggtgaaccgc
    65521 tgatttacgg tgtcaccctg ccccgcatga agccgtttgt taccgcctgt ttaggcggcg
    65581 cggcgggcgg tttgtttatc ggattgatag cctggtgggg tctgccgatg ggcttaaaca
    65641 gcgcctttgg gccgtctggt ctggtggcgc tgccgctgat gacttccgca caaggcatcc
    65701 ttccggcaat ggcggtttat gctggcggga ttctggtggc atgggtttgc gggtttattt
    65761 tcaccacgct ctttggctgc cgtaacgtca atctggactg atattatgaa acggacaatg
    65821 ctctatcttt ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata ccctgttctg
    65881 acagaaggta gttgatcaag tatatgcggc gttaaagcag taataaaaag gccagtcagt
    65941 aatgttactg cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcaacttgt
    66001 ccagcgacgg tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc aacatcgcat
    66061 cacgtttgcc atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac
    66121 gcgcgcagta ggcgcagaag tacagaccgt gagtgccact ggcggtgccg tacggcaggc
    66181 tctggcgaac aatcttcagc cctttgccat cttctttcag atcaacgcgg gtgaggtgag
    66241 aggtttccgg acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg cgcccgatca
    66301 tcatctcctg atcgtgaacg ctcatccggt tgagctgctt caggttgtgc tcccaacgct
    66361 gcacaaacac atagctgccg ccggaatcca cgccgtcttt gataaccgcc acttcgcggc
    66421 gcgtctcttc acccgccggg ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt
    66481 ccacccaacg gaagccgtgg atctcttctt tcacttcaat gcagtcacca aaggcttcca
    66541 tcgccgcctg agcgacagag aagtttacgt cgtgacgcag agaaagaatg tggatcaaca
    66601 catcgaactg ggtcgtcggc gcaaggcctt taccgtagcc cggaaaatct ttcagctctt
    66661 ctgccccaac gccgccgctc agagcgcgcc aggtgttgtt accaaaggca accaccgcac
    66721 caagatgcgc gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac
    66781 tggccgcacg cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc
    66841 ggcaatgttc tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataaga
    66901 atgccacgca atccgtggca ttgttgagcg ctattttacg tgttttttcg cgacggcggt
    66961 ttgttcagac gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt
    67021 atcgtccgaa ggcattaacc cttcaggacc acgccattcc ccgctgaact ggtagctaat
    67081 atgttgactg ccttcggctt tacattcgac ggcacgatta tcatcaccat cagctttttg
    67141 gcaattgcca aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt taacaccggt
    67201 gtcagcagga atatcgctat ccagcacgtc aatgcgactg atcgtgcctt gatcgccgtt
    67261 aatcaccatc gccacgttgt cgcctttcat cacttcaaaa aagcgcacca cgttgccgtt
    67321 cgtggttttc attccgctgc gcaggcgata atcgccatca agcgcatcag caatggcttg
    67381 ttcttgcagt ggtgtggacg ccgttaattc acccacgccc tgctcgctca ctttggtgga
    67441 cgatccaaac cagttccacg ggttagcggc agaccagtta actgacgaca tcgtggaaca
    67501 gccggtcagc atcaacggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc
    67561 tttggttatt caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt
    67621 ttcttcatta aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacaat
    67681 atccgccact accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca
    67741 cggttgccag caaagtaaaa ccacctgcgc caacagcaac agaaaataga gcacatgcca
    67801 ggtgcgagga aacgaagccc gccgaccgct caacaaaaac gccagcaccg ccggaatacc
    67861 gggaatcagc cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt
    67921 accctgctca cgcgacgcac cggcaatgac aaacagcacc caggttcgcg cctgaagcaa
    67981 tagcacaagc cagaagagca agggtaaacg caggcgtccg tgcgcatcat aatggacagg
    68041 atggaactca gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt
    68101 gttcatatcc caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacgttga
    68161 tctgaatttt cgggcagccg cgagcaatca gctttttctc cagccggtta agcaacgcat
    68221 tggcaattcc acgcccacga aactctggat gcacgccaag ataataagca gacccgcgat
    68281 gcccgtcata accgcccatc accgttccga ccacttcacc gtttacctca gcgaccaaaa
    68341 acaaactgac gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac
    68401 gcagcaagtc gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata
    68461 cgcgtatctc catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa
    68521 cggtcgaatt agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg
    68581 tcaactattg aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta acggattacg
    68641 cgatacgata taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa
    68701 accactaaaa acactcactt cgcgccgcca ggtgctgaaa gccggtttag ctgccctgac
    68761 gttgtcagga atgtcgcaag ccattgccaa agacgaacct ttaaaaacca gcaacggaca
    68821 cagcaagccg aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg atccaggcca
    68881 cggcggaatt gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct
    68941 ggcgattgct aaaaacgtcc gttccatttt gcgtaatcat gggattgatg cgcgtttaac
    69001 gcgttctggc gatacgttta tcccacttta cgatcgcgtt gaaatcgccc ataaacatgg
    69061 cgcagatctg tttatgtcaa ttcatgccga tggctttacc aacccgaaag ctgccggtgc
    69121 ttcggtattt gccctctcta accgtggggc aagtagcgca atggcgaaat acctgtctga
    69181 acgcgaaaac cgcgccgatg aagttgccgg taaaaaggcg actgacaagg atcacctatt
    69241 gcagcaagta ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgcttgg
    69301 ctcgcatatt ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca
    69361 agccgcgttt gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg aaacctcgtt
    69421 tatcaccaac ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc agaaaatcgc
    69481 cacagcgatt gctgaaggcg tgatcagtta tttccactgg ttcgacaacc agaaagcaca
    69541 ttcgaaaaag cgataagtta tgaaacccga cgcacaccag gttaaacagt ttctgctcaa
    69601 ccttcaggat acgatttgtc agcagctgac cgccgtcgat ggcgcagaat ttgtcgaaga
    69661 tagttggcag cgcgaagctg gcggcggcgg gcgcagtcgg gtgttgcgta atggtggtgt
    69721 tttcgaacag gcgggcgtca acttttctca cgtccacggt gaggcgatgc ctgcttccgc
    69781 caccgctcat cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt
    69841 agtgcatccg cataacccgt atgttcccac cagccacgcg aatgtgcgtt tttttattgc
    69901 cgaaaaaccg ggtgcagagc ccgtttggtg gtttggcggc ggcttcgatt taaccccttt
    69961 ctatggtttt gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgccagcc
    70021 atttggtgaa gacgtttatc cccgttacaa aaagtggtgc gacgattact tctacctcaa
    70081 acatcgcaac gaacagcgcg gcattggcgg gctgttcttt gatgatctga acacgccaga
    70141 tttcgaccac tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt
    70201 accaattgta gagcgacgta aagcgatggc ctacggcgag cgcgagcgca attttcagct
    70261 ctaccgtcgc ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg
    70321 cctgcaaact ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg
    70381 ggagtatgat tatcagccaa aagatggcag cccagaagcg gcgttaagtg agtttattaa
    70441 ggtcagggat tgggtgtaac tccctcaccc ccattcccgc atccgctgat gcaacgtcaa
    70501 cgacggcttc tcggcaaaca gctgctggta atccgtggca aattgcccca gatgccagaa
    70561 tccccactgc atggcggcgt cttttaccgt tgtgctttgc gaccacgggc ttatcagttc
    70621 gcggcgtacg acgttcaggc gaatgcgttt cagccacgcg tttgggccaa tgcctaaaat
    70681 agcgtgaaac gcgttttgta gcgtgcggcg actgacatgc agttgattac acaaatccag
    70741 caccgtcacc ggttcggaca tgttttccag cacatattca cgggcgcggg aaagcaaccg
    70801 acggtaactc tgatgactga tgctttccgc cgtcaccatt ggttgcgctt cttccagcat
    70861 cgcccccatc gccattagca aattatcccc cagcactttt cgcactgcag gctgatggag
    70921 attttccggg ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagcgcggc
    70981 tttatgctgc tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc
    71041 cggattgtgc aaaaagttag cctgacaggt gatgacatct tcagacagca ctacgcccag
    71101 gatcgtataa tcgtccggcg tacttaattc aaactcggtt ccaccggggc gcgtcgctat
    71161 ttctgcgctt cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat
    71221 gccaaaccag aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccggtgta
    71281 ttcacgaaaa acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc
    71341 cggatgcagc tgatcgtaaa tctgctgcta ggcggtaatc gtcaaagcat gttcatagac
    71401 atccgttgtc tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt
    71461 ttcgggtaag ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat
    71521 gttaatgccg ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg
    71581 ataacgacta ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct
    71641 ggctttttcc agcggccagc caaagtgggc ggccacttct cctgccgtca ttccctgaca
    71701 tactgatgtt aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc
    71761 gggttccttt acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc
    71821 atcgtctggc cgaccgatct ccttacggct gatgacacgc ccggtacgca tcgcggcagc
    71881 acaacctgcg tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttaa
    71941 gcgaccaggg tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc
    72001 atccgctgca tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat
    72061 catcggcaac ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc
    72121 ggtaaaggcg ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga
    72181 gtagttggtt tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg cagatttcaa
    72241 cgcggcatca atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc
    72301 gccaagcgtg atcccggcgg ttgatgaaag ataagaaccg gtacgcgaaa ccacatgcgc
    72361 caggaaggcg gtgttttctg cgtcattggc ccactggaaa gctgcgccat tttcaatatg
    72421 tgcaaccatc gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat
    72481 cagcacttca ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc
    72541 atataccact tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt aggttacgtc
    72601 gtcagaatcg gcagaaatca gcccgaggct acgaatatgc ggcggcaatt ttagttcacg
    72661 cgcaaattca gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa
    72721 atctaaagct ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc
    72781 cagcatacgt ttcgccaaat ccacaatgac ggcacagcag cacgaaagag aagcaaaagt
    72841 cagctacctg gtacactagg cagcgggtca gcaaaggggg gcaaatgccc cttttttcat
    72901 gtcagataat cagatcatta cctcaataaa ctaataatca catcgcggtt acaaccagtt
    72961 tgatagtgat agctcaaacc cagacgtggc gctggctgca agcaaaataa gccgcaaaaa
    73021 tgcgacccca tgtttactag ccgcaaaaat gcggtcctat ttgccagaac cttttattat
    73081 tcaattggtt agtgcaagaa aaagcgtgat tactgttcaa gaagtatcca gaaaggtcat
    73141 gattttgacg ctgaaaaaaa ataggtgggc atttttgccc actcactagc cgcaaatttg
    73201 cccactcact agccgcattt ttgcggccca aagtaataaa tacagagaaa taataaatac
    73261 agatcagcgc caagattttt cgtcttttcg gcggaatcat gagactagtg cttacgtcct
    73321 gataacctcc agagcagacc ttaacttctg tgcagcagga ctacgctcat tgccgctcgg
    73381 ctaggtcaaa tcctcacgat aaagctgttc ggattgtgac cataatgtta aaggactctt
    73441 taaatcattc cttgacgaat taactagctg gcttaagcgg aatagcatgc aggataaaaa
    73501 caaaaccagc ttagcagtgg ggagagcttc gcttcgctcg tctcctgggg ccaatgctca
    73561 atgaaacgac gtaaggcacg gagcgctcac cataggtaga cacataaaca tctgagagcg
    73621 caccttctat cgcgctacgc gctcaataaa aaccaacgac accgccacac tgcaagacca
    73681 atggttttta tcgcgtatac agaccagaca ggaaacagca gcaaagcagc gtatagccag
    73741 gtgcagacag cacactcaca cgacgcacag caggcttaag tgatcactca ggaaacttta
    73801 tccccgccgc cgatcaccgc gaactatcat gaccgcgcca aattcgaaag gaatggccca
    73861 tagaacgcga tgaacacaca taagcgctac acttacttaa cgtcgccaca gagcgattga
    73921 gagcgcttaa aatcgatgcg atttcattaa atttttctca ctcgcctgaa tggtggcgat
    73981 caggactgat cgcaacattg ccccgcctcg atattgagcg cataatagat acatacatta
    74041 agcggaaaag agataagttt attgtgaaaa tcagggggtt acatatcatg cgcatatcag
    74101 aacaacaacg gcagatcctc gactatctgg caaaaaacga atcaatcacc gcacgacaag
    74161 ccgcagatct cgtttatggt ggcaatgtta cccgcgtcca ggttgacgcc gcccgccgct
    74221 cactcatgac gatggttagt aatgggctaa tcaggaaaca aggccgcgct ttcgtagctg
    74281 aaagacatat caatcacgct tatagccagg acgttgaaga attgcgccgc cagatccttt
    74341 gggtattcag caaacagggt caggagtatt tcctttctga acacccaaat ttcgccgcca
    74401 aagagttaac agtaagcgga ttgtcgtgcg cactggatta tcttgtcggt gaaggttgcg
    74461 ttacttacaa gcagctatgg cacgccctga atacgcttgt agaagatggt ttactcattg
    74521 ccacgcgtga gaacatcgaa tactggataa tgccgcgttc aggtcgccgc aatcatgcac
    74581 ggtatatttc aggcaacaga tacagaacgc cggactttaa tccgacacag gaagatcggg
    74641 accgtgccga aatgatgtgc agggaacagg agaaaagaat tatggataag ttattttcga
    74701 tgcagtgaac acaagcgggc gcaatgcccg tttttattgc cttattccac gcctgacgaa
    74761 tcaatttgat cacacctgag aaaatgcata gccagaagtt aaggccgtta tattgcgatt
    74821 gggagcgttt taaacggtat caatttaatt aattctgatg tggtgatttg caggcaaaaa
    74881 aaacccgccg gagaagcggg aaatagagac atatcgttgt agttattaac agatcacttt
    74941 gaggtcgagg gcggctaacc ctcgctaata ttatcccgct attaccttgc gggtgcaaat
    75001 aatcttttat tgctttgatg atttaataaa tagcactttt tgctaacaaa aaccgctgtc
    75061 aatatatggc caaaaatgtc aacagaaaaa ataagattta taatattaag tatcacatgg
    75121 gtaatgggtt cagggaatcg gaatccaccg cctcacggaa attcaaaatt tgcttcttct
    75181 ttcctccgtg ggcggcttct ttttcgtatc gaggtattta tgaaagcaat aacattagaa
    75241 cagttcgcag caatggcgaa tgtatcgacc agaacgctga ggcgctggct aaaaaaacca
    75301 tcaaccggag tccctttgcc atttacccca cgcggtactc gccaattatt ctgggagcac
    75361 gaatgcaaag aatggttgta cagaacagaa cgataataat cacgcatcac attttgaggt
    75421 aaacatcatg accaatatcg aaagattaga acgctgtgcc gatattatgc gacgcagatg
    75481 gatttatgac cctgaagcgg gtttattgtt tagccgtgaa acaaaagacg ttatcaaagg
    75541 cagcatgacc aataaagggc atttggttgt aacggtacat gaaggtaaat tcaaagtgac
    75601 actcacctat cagaaagcct gctatgtata cacatacggc gcttatgacg agacattata
    75661 tgaagtgcac cacgttaatt tgaacaaaca ggataaccgc atcaagaata ttcgcctgat
    75721 gccgaaagaa aagcaccgcc ggatacataa aaacgtccgc aaacttattc ggctgggcca
    75781 cacacaatgg cttcaaccgc aattaattga acaatcacta taaacacagg agtaaatacc
    75841 atgcattcat tagacagtta ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa
    75901 cgccgtgagg aattccatga gaaagtgatg cgttctgctg attatattgc ggacaagttc
    75961 gtggaaacgg ttcgtccact tgttgatgaa gtagctgata aacttcagtc tgaaatgccg
    76021 gaagatatgg aaggcactgc aaagcggcga ctcatttgcg aattaagtcg tcgttttggc
    76081 gtgtcaataa gcgcttttaa ataatcgaaa aaagggggga aagataatgg acgcatttga
    76141 ggtactggca aataaaggcg ctgaactggt agcacagcgt gataaaaccg ccaacgaagg
    76201 ggaacgcgca ctgctaaata aacagattaa agcgatccgt atggcccagt ttaaattaat
    76261 cagtaatgaa gttatcgaaa caattaaacc gcagatccca caaatcgttg ctgacgctat
    76321 taaagccgcc ggactggaga aacgcattgc cgggctaaaa acgaacgcag agaaaatgga
    76381 gatgtacaaa cagagcggtg ctccgaatcc cgatgagtac ttctcaccgg atgttgagtt
    76441 tatggcgcag gttgaggaac gtctgggtag ctgtctcacc gaggaacagc gtcgctattt
    76501 tgatggagtg gatagttcgg caggtattga cctgaactcg tatttcggtc gtgaaattga
    76561 gcattttgat gccgccccgt ctatgccgga gccagaacca gaaatggcca ccgatcctga
    76621 agctgagcaa cggaaacgcg tattcaatgc gatagggatt cggtactgat cggataaaag
    76681 aaagccggga attaccccgg ctgacttaac aatgtgacca atgaacaaat cggacaagac
    76741 aaacaggata agcagtctcg aaacaatgtt cgggaactat attagcacaa gtccggagaa
    76801 aatcatcatg ttggcactac gcatcatgca gggcatcgct aagaccctcg cagaacatgt
    76861 cttagacctg aaacattcgc cactgagcaa acaggccatg aagcgccaaa cgcttcgtct
    76921 gtgggctgaa tattcgcttg gaactatcaa taaaatcatc gacatgaaat cggggccgag
    76981 caaccagagc gcggaggaaa tggagttcat caggcgttta attctcattc gcagagacat
    77041 tcatagccag ttacacagcg tgggaatcga tattaatgat gggacgggcg attaattttc
    77101 ggacaagtac tatgcgcgcg cgaggagctg aacatgaagc aagatattgc agacaggctg
    77161 gagatactcg aaggccagcg ggcagaagcg aaacaactac gtaagcaggc cagaagagcg
    77221 cacaggaaca acgaagcaga gttactaacc aaatacatca gtttcaccaa ttactgcatc
    77281 tacgagtgtt acaaggaaga tgcagaggac tggctggatt cattgccaga acagtactga
    77341 gcagaataac accacgtcat gttgttctga ggtgagcata tcacctctgt tcaggtggcc
    77401 aaatttagtg tgccacttca gcatcaccgt tgaaatcaca ataacgattt ttttgtaaat
    77461 atgctctcac atatgtagga atagaccagg ttggcattga aacacatcta ccatgaccat
    77521 aataaagtaa ttttttgata tgtttatggt tttatattaa ttaaaatata tctaaatttg
    77581 atagttttaa aattgcaaaa tccagattgt gtttgtaatg tttaatctga ttgtattgag
    77641 tggcgtttat tatgaaaaat aaatcaggta attcttgtta tagagaagca gtcgatattc
    77701 atagctcact tgagctttca tttatattgc ataaatatca tttgcgtgta gatgagaaga
    77761 aatctcgaaa tgtattcagc gccatacgtg atgagctcat ccttgatggg gattcacgac
    77821 aaaacttagc tactttctgt caaacatggc ttgatgatga aatccgtgag ttgcttaatt
    77881 tagctattga taaaaacatg atttataaag atgaatatcc acaaatggcg gaacctgaag
    77941 gtcgctgtgt gcgtatgctt tccgattcgt ggagtttccc cgattcgcga cacaccttag
    78001 gttgctcaac cattggttca tctgaagctg ctatgctcgg ccgcttggcg ttaaaatggc
    78061 agtggtgtaa aaaacgcgaa gtacaaggta aatcaaccga acctgatttg cgatcctgtg
    78121 caaatatgct ggcataaatt ctgccgttac tgtgatgtga aattgtgtga aattccactt
    78181 gatgatcatc acctgataat gaatgctaaa gaggttttga agcatgtcga tgaaaacact
    78241 attggtgtcg ttacgacttt aggtattact tatttttgta aagcagattg cgttagtgtt
    78301 tttaagataa gtgttaaagt taatattagt ttttatattt acatcgtaat tatatgtttc
    78361 tattttaaaa atgttcgaga gtataaaaat gtctaatgct aatatcgcac ccaaaagtaa
    78421 gaaaatgaca gtggggacac tcgctatcat gaatataact gctgtagtaa gtctacgatt
    78481 tataccttct gaagcagaat acggcttagg tgccattttt tactatacct ttgctgcaat
    78541 tatgttttta gtacctgttg cctttgttgc ggctgagcta gctacaacat atccacaaaa
    78601 agggggggca tttcgatgga taggagaagc atttggggct cgctggggtt ttgtaggaat
    78661 gcttatgacc tggctacaag tcatccccta ctttccaaca gtactgacat ttggtgcagt
    78721 ctcggttgca tttattgatg ttgatatggg ggtggcagaa tcaattgcca gtaataaatg
    78781 gtatatttta tttttcgtac tatttgttta ctggggagct atttgtgttg cattacgtgg
    78841 cgttggtatt ttctcgcagg tatctaagtg gtgcggtatt atagggacaa ttattccagc
    78901 tattatcctt gttattcttg gtttctccta tctgtggttc agcggtaaac cagcggtgat
    78961 cccattaggt tggggtgatc ttttccctaa tatagctaac ttccagaata ttgttcttgc
    79021 cgccagcata tttcttgctt atgcagggat ggaaatgaat gcggtccatg ttaacgattt
    79081 agataatcca actaaaaaat acccaattgc aattaccatc gcttctatag gtactattgc
    79141 aatattcctg ctcagtactt taggggtcgc ctttattatt cctacagata aaattagtct
    79201 aactcaaagt ttgttggttg cctacgatat gctgttcgaa tgggctggag tgccatggct
    79261 tggctctgtg atggcattta tgctagcaat tggcgtactg ggtggggtgg ttacttggat
    79321 tgcagggcct aatacaggtg tattagcgat cgcgaaagct ggttatttac ctaagttttt
    79381 ccaaaaaacc aatcgtcatg gaatggggca ccatctaatg tttgtacaag gtataattgt
    79441 ctcagttctt tctgttactt ttgtgataat gccttcagtt caagcagctt ttcagatttt
    79501 atcacagtta acagttatgc tctatttagt tatgtatatg ctcatgttcg ctagtgctat
    79561 ctatctacga cattctcaac ctgatacaaa acgcccatat cgagcccctg ctttattttt
    79621 ctgggcaaca cttggattct tagggtcttt tctggctttt gtattaagtt ttattccgcc
    79681 cgagcaaatc ccaactggta gtccaaccag ctatgttctt tatttgttag tattagttgc
    79741 cctattttta ttaattccaa tattaattta cgccttccga aaatctgact ggaaagatcc
    79801 aaactctgat ttcgaaccat tttcttggga aaaaagaact agtaattaaa ttttccataa
    79861 gctacaaaag atgaggttaa atatgtttag tgctgaatct ccaattggtg taatagactc
    79921 aggtgtcggt ggattgacat cggtaaagga aataattaat ttgcttcctg gtgaagatat
    79981 tatctattgt ggagataata tgaatgcacc ttatggcaac cgttctgccg atgatatcat
    80041 cagtttgact aaaaaaatgc tgactttttt acagtcaagg aatgtcaaac tagttgctgt
    80101 cgcatgtaat actatatcct caactttgga atccgaagaa tacagcggat atgctaagag
    80161 ctttccgttt cctatcttat ccatcattga acctgcagta gaagatgtga ttcgccagca
    80221 atataaaaat gttggtatta tcgctaccga gtttactatt aaaacggggt gtcacaaaga
    80281 gctaataaaa aaattgaatt caacaataaa tgtatttgga gaaccaagca aaaatcttgc
    80341 aatgttaatt gaggaaggta atcttaatgc tcctgcaatc ttgaatgaca tcaaaaaaca
    80401 tgttaaccat ttaatttccc tccatccagt aaatgaaatt attctcggct gtactcacta
    80461 tccaattgtt cagaattttt ttgaagcagt tgcaccggat ataaaattta ttaatcctgc
    80521 acatgatcag gctatctcta ttaaaaatca cttgggacag ctaaatttat taaactcctc
    80581 aaatatagga actttgcaaa taaatactag tggaagtatg gagatctaca aaacggtact
    80641 aggggaattg agcattacga aacctcatac attcagtatc aggcagtttt aaatttatcc
    80701 ggtacaccgt aaaatcccgt ccttctgaac tgcaccccaa aagttggaca ccaactgagt
    80761 aaggtgcatt ttatggacaa acccagttac atactgggta gtgcatccaa ttagtagaac
    80821 atgtgttttt cgataaacgc tccgatcact ttttcgtgga tctcaactga gcgtgagaag
    80881 cagattgttt tacgacccaa ccgcttaatg cgggtgcgta gcgtcagatt attacgctca
    80941 atgcgttggg tgaatatttt gccggtcaga tgcttattct tcggcacctc ccggccatag
    81001 ctgccccagt catcgctggt cagcatgccg atgttgaagg gtgtaagcag tgccagtagc
    81061 tcccggcacg tttgatcggt tcggggacca aaagtgtagg ccagtacacc gcctgttttg
    81121 gtgttgtacg cgtacccgag ccaatgttgc cgggctttac tgccaacgta gctccattgc
    81181 tcatcaagct cgcagataaa cgccacatca gcatgggcaa caggcgaaga agttattcgc
    81241 tttggcgcga gttttttaaa gtccggatga cggtgttact accaattttc agtgtcctgg
    81301 cggtatcgcg aaccccggca ccattaaagg ccatttcagt tatcagctct ttcatacccg
    81361 gcttacgtgc ttgataagta taagtgaact gaaacacacg gtggcagtca cggcagcgaa
    81421 gtctgtcacg gcctttaggg ttctgaccat ggcggtaaac ctgagctgac tgacaacggg
    81481 gacaatgaat attaacgctg gtcatgagat aacctcaaaa gcccgtatta tacatcagat
    81541 tcaactaatt agaggcatca cccatccagc attataaaac atactaacaa taactaaatt
    81601 ataataatca caacttacaa tcaaataata aatgcataag attaacaatg attttttcgg
    81661 gttatattta tctaaaaaat cagaacatta tgtaacttat tattttctaa gcgaaaattt
    81721 attgtgcggt cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta
    81781 ttctttcttt tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctgttc
    81841 tggttttggt tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa
    81901 aacatttttc accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata
    81961 cactgccgga tataatccca ctaacaccca gacaccaagc accatacctg ataactaccg
    82021 tattcaggaa ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag
    82081 aggcgtacca atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa
    82141 cgggagttgt tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc
    82201 ggccaccacc agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt
    82261 gatgaccgtc agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta
    82321 cacgtcagtt ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag
    82381 atccgtttca tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc
    82441 cagccagatt gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtgga
    82501 gcgtacggtt tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag
    82561 cgccgttgcc agaagtgggc caatatcggc aaaacagaac gggttggcgg gctggtggcg
    82621 ataacattcg cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag
    82681 caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac
    82741 aatcagcgcc gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc
    82801 gtcagtgcct gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac
    82861 cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc
    82921 gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt
    82981 cagtacccga cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat
    83041 cacggattcg aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc
    83101 cttcatcact ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag
    83161 ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc
    83221 gggcacggac gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag
    83281 atccgccact tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc
    83341 ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg
    83401 cttcctcctg tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt
    83461 cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg
    83521 ttgcggcgac gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc
    83581 tgacgccaat atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga
    83641 catagatcag catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat
    83701 ggctaaaata ttcacccggc gtatcaatat cgcccttatc attaaattcc acggccagtg
    83761 tttttctggc gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc
    83821 cgacgctacc cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt
    83881 cacaaagcgt ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt
    83941 ctactgcgcc caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga
    84001 tatgcacatc agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag
    84061 ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct
    84121 caccgggatg cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa
    84181 tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt
    84241 tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcaata
    84301 agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat
    84361 aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa
    84421 gctttgataa tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag
    84481 ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg
    84541 acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc
    84601 agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg
    84661 cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc
    84721 ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca
    84781 atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac
    84841 gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata
    84901 ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct
    84961 ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgca
    85021 ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacacta
    85081 aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga
    85141 accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga
    85201 gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga
    85261 tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc
    85321 tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc
    85381 gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg
    85441 cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa
    85501 acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg
    85561 cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca
    85621 aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa
    85681 gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt
    85741 ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg
    85801 aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca
    85861 tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc
    85921 aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg
    85981 gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca
    86041 tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc ctgatagata
    86101 acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg
    86161 ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg
    86221 tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg
    86281 tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag
    86341 aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg
    86401 gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca
    86461 atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc
    86521 gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta
    86581 ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag acaaggtgcg
    86641 gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga
    86701 gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac
    86761 tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca
    86821 gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa
    86881 gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc
    86941 tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt
    87001 tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta
    87061 tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc tgcgatattg
    87121 agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg ttactcaagg
    87181 ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa
    87241 atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg
    87301 gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct
    87361 cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac
    87421 aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg
    87481 cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttctttta
    87541 cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta
    87601 tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg
    87661 gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg
    87721 tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac
    87781 tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta
    87841 aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc
    87901 gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt
    87961 tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg
    88021 gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgct
    88081 gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa
    88141 gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac
    88201 cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc
    88261 ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca
    88321 ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa
    88381 ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac
    88441 cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg
    88501 atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg
    88561 attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc
    88621 gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt
    88681 cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg
    88741 tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa
    88801 gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc
    88861 tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa
    88921 aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat
    88981 ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa ggactgggag
    89041 aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt
    89101 aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt
    89161 tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc gctgaaggaa
    89221 gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc
    89281 aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt
    89341 gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt
    89401 tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa
    89461 ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcagatggaa
    89521 gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc
    89581 tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt
    89641 ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt
    89701 tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta
    89761 cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag
    89821 tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac
    89881 tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct
    89941 gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa
    90001 gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc
    90061 cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg
    90121 catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg gcccatgcca
    90181 gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg
    90241 ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc
    90301 acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctggccgtt
    90361 gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac gcgacacaat
    90421 ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagtgcttt
    90481 atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag
    90541 accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga gtgagagtgg
    90601 cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag
    90661 aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc
    90721 atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat tgccgtcgcg
    90781 taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg ttgccgtatc
    90841 atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg ccaacggctc
    90901 accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc
    90961 accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc tcagggtcat
    91021 caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat tattacaggt
    91081 gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa
    91141 caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt
    91201 tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcggatttg
    91261 tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc cattaaatgt
    91321 gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg
    91381 atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca
    91441 ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc
    91501 tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca
    91561 tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc
    91621 agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca
    91681 cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag gatcgtcgca
    91741 ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg
    91801 agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg
    91861 agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta tattacgctg
    91921 tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca
    91981 gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc
    92041 accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg
    92101 gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag
    92161 ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac
    92221 aaattcactg ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag
    92281 acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct caagcgccac
    92341 cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt
    92401 cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg
    92461 gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt
    92521 aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt
    92581 agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa
    92641 acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa
    92701 aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat
    92761 cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat
    92821 gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca ccgccacgcc
    92881 attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga taatcgccac
    92941 ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg
    93001 atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat cgcgaatagt
    93061 acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt
    93121 gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat
    93181 ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgaa
    93241 cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc
    93301 atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt
    93361 tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg tactggcatg
    93421 ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca tgccggagag
    93481 tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac aggcaggacc
    93541 gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc
    93601 gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc aggatttaca
    93661 gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt
    93721 agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg
    93781 ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg
    93841 ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg
    93901 actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat
    93961 tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt tcatgtgccc
    94021 agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa
    94081 attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt
    94141 taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat
    94201 tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat
    94261 caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg
    94321 cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt
    94381 aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa
    94441 tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc
    94501 caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc
    94561 tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac
    94621 gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc tgaatcagct
    94681 ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag
    94741 tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga gcgagctgca
    94801 taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgagac
    94861 caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa caccagccag
    94921 attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa
    94981 tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag
    95041 ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg
    95101 caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga
    95161 cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa
    95221 cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt
    95281 atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc
    95341 atggccgatt tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact
    95401 actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat
    95461 acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca
    95521 ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg
    95581 atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat
    95641 cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt
    95701 cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt
    95761 tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggtgag
    95821 gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt
    95881 taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc
    95941 atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag
    96001 tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat
    96061 ttctttattg atcgccccat ttttgcctgg gtgctgacaa tcctgttgtg tctgacaggt
    96121 accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg
    96181 cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag
    96241 gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt
    96301 ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc
    96361 gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa
    96421 aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc
    96481 tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag
    96541 gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc
    96601 atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc
    96661 actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct
    96721 tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca
    96781 gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc
    96841 gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt
    96901 aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat ggcgacagcg
    96961 gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac
    97021 aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg
    97081 ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc
    97141 gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt
    97201 tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt
    97261 ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa
    97321 ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc
    97381 gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc
    97441 ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg
    97501 gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt
    97561 gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc
    97621 gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt
    97681 tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta
    97741 ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa
    97801 caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac
    97861 aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg
    97921 gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg
    97981 tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc
    98041 gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg
    98101 caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg
    98161 ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg
    98221 cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat
    98281 attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc
    98341 ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac
    98401 atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg
    98461 acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt
    98521 gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa
    98581 tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag
    98641 gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc
    98701 ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg
    98761 ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt
    98821 tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc
    98881 gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc gacgctccac
    98941 gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta
    99001 ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc
    99061 gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt
    99121 gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg
    99181 acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg
    99241 ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag
    99301 ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa
    99361 caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa
    99421 accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt
    99481 cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac
    99541 aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat agttgttaat
    99601 ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa tgttttcacc
    99661 cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta cactttacgg
    99721 catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca ataacatcga
    99781 ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga acggttttat
    99841 caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc gtaaactgga
    99901 cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa tgactgaaat
    99961 gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc tgctgggttt
   100021 cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt gcccggttat
   100081 tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg caggatgtca
   100141 ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca tggattttgc
   100201 cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct ttgccgggca
   100261 taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt ttgaacccac
   100321 cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct cgctggcggc
   100381 gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg ggcgactggc
   100441 atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa aagtcgtcga
   100501 ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac cgtcgagtat
   100561 cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct gcaacctcac
   100621 cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc cggtacatcg
   100681 cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca atgaattctt
   100741 cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta acaatgttat
   100801 tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga ctgatgagat
   100861 gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata cggtggattg
   100921 gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa
   100981 cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg gaacgtccga
   101041 cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac
   101101 tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg cccgtatgta
   101161 tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg aaataagcat
   101221 ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag gcgtcctgct
   101281 gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca acaagccgga
   101341 gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact ggccgaaaaa
   101401 ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc gttaacgccg
   101461 catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca ggccaggtaa
   101521 tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc acttagcgct
   101581 tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa ttgcatactg
   101641 cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc accaccggca
   101701 ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg atcttcacca
   101761 ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct cgcatagcgt
   101821 ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct ctccagcgcc
   101881 ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac tccgctctgt
   101941 ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac acgcgcctgg
   102001 cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg attaccgctc
   102061 ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc aaaccagctg
   102121 cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag taacaactgt
   102181 tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc gaactcctta
   102241 actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa attgaaggag
   102301 tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc gaacctcatt
   102361 tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca tcttgcggcc
   102421 tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta aacgcccacg
   102481 cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac ctaacgatgt
   102541 taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt cgtcagaaag
   102601 gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg cgagagcttg
   102661 cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg catcactcat
   102721 cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat tacccatccg
   102781 caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt aaccaaaact
   102841 caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa gttgtcgccc
   102901 tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc ttccgcgcaa
   102961 tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca gattaccccg
   103021 cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc cgccctcatc
   103081 caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa aatgttgccc
   103141 tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg caccagacaa
   103201 taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt gttcaaatgc
   103261 ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa aaaccagtgc
   103321 ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct gttgcagttc
   103381 aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac gtccggtacc
   103441 ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata ccagttgatg
   103501 caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg cttgctcatc
   103561 gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa attgtgccag
   103621 tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa tacgagaaat
   103681 gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg ctgccacgcc
   103741 cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg gtgcgccggt
   103801 cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct ggttttgccg
   103861 ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga caaaatgcgg
   103921 cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag gttggttttc
   103981 ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca acgttccgct
   104041 aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg catgccggaa
   104101 ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag catctagctg
   104161 cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag catgatcaaa
   104221 acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac gtttcatttg
   104281 cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa ttaaatgctt
   104341 ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt aaaccagctg
   104401 tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc ctgactttgc
   104461 tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc cagatcgcca
   104521 gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa acagtcggcc
   104581 tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt ttgttgcagt
   104641 tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac ttcatgggcg
   104701 ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat gtcatcatag
   104761 aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc cataatggac
   104821 tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt cggttgctga
   104881 taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt tgcctgaatc
   104941 accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt tgattgttgt
   105001 tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta gcctgcaacc
   105061 agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa accgggaccg
   105121 cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact ttcacgtcgc
   105181 ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc gtaggtggtt
   105241 cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc gttcaatgca
   105301 tatattccaa gagcggttgt cttgccgtgc cagctgcacg gcaagatgat gaacagaaaa
   105361 atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg atcaggccac
   105421 cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg tcccacaggc
   105481 ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac agaccaaact
   105541 ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc agcacgtcgt
   105601 tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc cagccaaagg
   105661 tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc aggaacagat
   105721 cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc actagagagc
   105781 cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt ttcaccagac
   105841 attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca gccagtttgc
   105901 tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac tgctcaatgc
   105961 gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg tccgggtttt
   106021 ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt tgcatcttta
   106081 tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg ccggatgttt
   106141 tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat ttcgtgcgaa
   106201 ttaccgctaa aatcgcgttt tcctgccgac gcacacgttt gcgtatcata tcagaaaaaa
   106261 gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga agacagctac
   106321 cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga actgtaggct
   106381 gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca ggcctggatc
   106441 gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta cggccatgtt
   106501 gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg ccgctggcag
   106561 acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac cggtgtcatc
   106621 agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt tcgcggcgtc
   106681 agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc tgtaacgctg
   106741 catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca gtttaaccgt
   106801 aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac gatactgctc
   106861 gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac cagcatcatc
   106921 acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg gccacagagt
   106981 attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact gggtacgcgc
   107041 gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct taccgacagc
   107101 accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca aaatcattcc
   107161 agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat caccactgac
   107221 ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa gcgaaacacc
   107281 cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc cctaaacttt
   107341 acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg tcggtgattg
   107401 gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta catgcccatt
   107461 tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc agacgctgat
   107521 taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc tgggccatat
   107581 cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat tgcatgaagt
   107641 ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc agctctttga
   107701 tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata ttttttactt
   107761 tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac ggcacattat
   107821 acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga tacaaacctt
   107881 cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata ccactgtcat
   107941 tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc ccggcaatta
   108001 ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca tgttcgtcat
   108061 gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa gtaccgctgg
   108121 cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct ttttcatcca
   108181 tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg tgcaaacaag
   108241 tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg ttctgattgt
   108301 tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa ggaataacca
   108361 gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc gatcgccctg
   108421 gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt gaagacagtc
   108481 gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt tcatggaatg
   108541 ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat cttttaatcg
   108601 tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc tgggttcagg
   108661 tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc gacgcgcatc
   108721 atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa cgggctgcgc
   108781 agttgcatat tcagataacc gcccacagcc ccgcatctgc ggacgcagca aatattgagc
   108841 aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac gtcgtcaatt
   108901 attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag ccggtgatat
   108961 cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga ccgacttcca
   109021 gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct gtaccgtaca
   109081 ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg atgtgctggg
   109141 tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc tgcttaactt
   109201 tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct ggggtgaaaa
   109261 gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga ttgacgctga
   109321 tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg acgttgtgct
   109381 gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc tgcgcttgcg
   109441 gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct gcgacacggg
   109501 tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt tcagaatcca
   109561 gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt tatacaatat
   109621 gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg tggtggccga
   109681 accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg tgcataaaac
   109741 gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa tcactgcccc
   109801 tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg tcaatgccat
   109861 ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct gcaagctttg
   109921 cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg tgaatgccca
   109981 tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa acgtgccgcc
   110041 ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga aatgcgacct
   110101 gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc aggcgttacg
   110161 gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc ttgccgcaag
   110221 ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac actaatggat
   110281 gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct ggcttcgctg
   110341 tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc gctggtgggc
   110401 ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac tttagtcgcc
   110461 cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag cctctcggca
   110521 tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc attgacttat
   110581 atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa tcttttcatc
   110641 gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt gctgtttgaa
   110701 atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac gtcgatcaac
   110761 gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat gatcgccttc
   110821 ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg ctttcacttt
   110881 ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc gcgaaagccg
   110941 ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca tcgcacgctt
   111001 cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg aaagtagcga
   111061 tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg atggcgatcg
   111121 gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat atcaaacggc
   111181 tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc ggtgtggcga
   111241 tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc ggcgcactgt
   111301 ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg gacgcgatta
   111361 tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa cggatgccgt
   111421 ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct ccgctgaatg
   111481 gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt gtggaagccg
   111541 tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact ggtgggctgg
   111601 cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg cgcagtacac
   111661 acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta ctgctcgcgg
   111721 cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata atgcatattg
   111781 cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca cttgtacccg
   111841 gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg gcgatgcctt
   111901 tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt cgtcgcacag
   111961 gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca ggcaatggcg
   112021 tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa cagctcgacc
   112081 ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag agcacagaac
   112141 ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg ctggtcaaag
   112201 aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg gtcgccgcac
   112261 tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca aactctttgc
   112321 gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat gctaacccca
   112381 ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg cgggccgggg
   112441 atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt tgcgccgaca
   112501 tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat gctggtgctg
   112561 gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg cgatctgatc
   112621 acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg gctggatacc
   112681 ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat tctggtcgaa
   112741 ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac gcatatcgag
   112801 atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt actggcgtta
   112861 ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga tgttgctgaa
   112921 gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg gctggcgcta
   112981 gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt ggccctgttt
   113041 ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc acttgtcgtt
   113101 acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa cacgctggca
   113161 cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct tgctgcgctt
   113221 gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg aaaatcttgc
   113281 tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt cgccacaacg
   113341 tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca tgttgtcgct
   113401 gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg tcaacgtggg
   113461 ggatgtggca ttatttggcc tggtcattga tcgcgtgagt acgctgattc tgtttgtggt
   113521 ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg ataaaaatcg
   113581 cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt ttatcggcgc
   113641 gatggcggga ctggtactct cctcgacgct gctcggtcag ttgttgtttt ttgaaattac
   113701 gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc agcgttcagc
   113761 actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg ccgccgccac
   113821 gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac atggcgacgc
   113881 acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg cccagctacc
   113941 gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg cctatctcca
   114001 cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta tcgacggcgg
   114061 caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca ccattcttta
   114121 tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct ggtcgaccat
   114181 cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct cgcggctggc
   114241 gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc tgtttttcct
   114301 tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc tgcgtggcgt
   114361 attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg caattaccgg
   114421 tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg gttttgcgtt
   114481 gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg aatcggtcgc
   114541 cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac cgagcgaggc
   114601 cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg tactgattgt
   114661 gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag atgatgaatg
   114721 accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc gctgtttgtg
   114781 attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc gctggtgctg
   114841 gtgtctattt tcgccactct ctcgtgcctg ttcgccacag agcaactgct gatctggtcc
   114901 gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta cgctgcacga
   114961 aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat ggcactgctc
   115021 gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct accgatggct
   115081 accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg cctgctgtgc
   115141 attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat ggaaaacggc
   115201 tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga aataggtatc
   115261 gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa aatatggcgt
   115321 acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg agatgagtta
   115381 ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc tctgttttgc
   115441 ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata gcttagggat
   115501 cacactgctg ctgattctag cactctgggt ggtccaaact gccgctgatg caggagaaat
   115561 attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc tcgccattct
   115621 tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc atgaagtggc
   115681 acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc atctgttttt
   115741 gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg cggcgatcga
   115801 agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt catcgctgga
   115861 atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc tgttcggtac
   115921 cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg cgatattctg
   115981 gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt tggcctttgt
   116041 gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg cctggctgcc
   116101 ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg tattgctgaa
   116161 ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca tcggcagcga
   116221 tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg cggcattttt
   116281 cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg agaacatggg
   116341 gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg cgctgctgca
   116401 caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca atgtactgct
   116461 caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca tgccatttac
   116521 cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc cgcccttcaa
   116581 catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc acctgctgat
   116641 tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac ggatggctgc
   116701 gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg gctggttgac
   116761 cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc atattccaca
   116821 acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga cgcacgacct
   116881 gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat ctgtttcgga
   116941 gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc gccgccctga
   117001 aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa caggtcacca
   117061 ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat caacatgatg
   117121 gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat tacgcggttt
   117181 attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag gcactggtcg
   117241 atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg gtctggggcg
   117301 agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat cagcgtcgcc
   117361 tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat gcgatggatt
   117421 atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc aatgagggca
   117481 acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat gaaccgggtc
   117541 acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc ctgttttatg
   117601 tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa gtgaccttct
   117661 tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat accaactcgg
   117721 ttgaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc tcgattctgc
   117781 tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc cattttgttg
   117841 gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg acgatggcgg
   117901 aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt gttcgccgcg
   117961 atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg cgcgccgacg
   118021 tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc actcagggca
   118081 ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg ctgatccgcg
   118141 cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg gcaatattcc
   118201 aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg tccgtgtcaa
   118261 agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc cggatacccc
   118321 actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct ttgttgaagc
   118381 gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat tgttccgctg
   118441 gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc tgcgcggcaa
   118501 taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact cctgtaccga
   118561 ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt ataaagagat
   118621 cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga agatgctgaa
   118681 gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct tcgcgccact
   118741 ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat gtattgcctg
   118801 cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca
   118861 aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga
   118921 agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg tcacccataa
   118981 agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc
   119041 gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc
   119101 tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg
   119161 cgcaacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt
   119221 ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg
   119281 aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc
   119341 ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa
   119401 cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc
   119461 gcagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat
   119521 aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac
   119581 agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg
   119641 ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag
   119701 attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag
   119761 atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt
   119821 caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat
   119881 cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa
   119941 ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac
   120001 gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat
   120061 gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt
   120121 ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg
   120181 acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc
   120241 cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt ggcaagcgcg
   120301 tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat
   120361 ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca
   120421 gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc
   120481 gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg
   120541 ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat
   120601 taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg
   120661 gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga gctacgcttt
   120721 attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa ctggctggca
   120781 cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca ggtggcgctg
   120841 cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg
   120901 ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt
   120961 ttcggtctgg cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc
   121021 ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa tatgcctggc
   121081 gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca
   121141 gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc
   121201 gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg
   121261 gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc
   121321 gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac cgatttgcag
   121381 gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc
   121441 ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt agatattgtg
   121501 gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc
   121561 gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc
   121621 gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt
   121681 actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt
   121741 ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag
   121801 gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt
   121861 gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc
   121921 ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat
   121981 atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt
   122041 gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg
   122101 cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat
   122161 ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt
   122221 cgcagttcaa tggcgtagtt gctgcacccg acgacgccag agaatgacga agaagaacgt
   122281 cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg
   122341 gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc
   122401 tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa caaactctct
   122461 ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc cgcccataaa
   122521 attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt gtttatggcg
   122581 atgtccgctg gcgtgtttat ggcgatcgga tttacttttt acctttccgt tatcgccgat
   122641 gccccgtctt cataggcatt aacccatctg gtgggcggcc tttgctttac acttggcttt
   122701 attttgctgg cggtttgcgg caccagcctg ttcacctcgt cggtaatgac ggtaatggca
   122761 aaaagtcggg gcgttattag ttggcgaact tggctgatta acgcacttct ggtggcctgc
   122821 ggtaatctgg caggtattgc ctgtttcagt ttgttaatct ggttttccgg gctggtgatg
   122881 agtgaaaacg cgatgtgggg agtcgcgatt ttacactgcg ccgagggcaa aatgcatcat
   122941 acatttactg aatctgtcag cctcggcatt atgtgcaatc tgatggtttg cctggcgctg
   123001 tggatgagtt attgcggacg ttcgttatgc gacaaaatcg tcgccatgat tttgtccatc
   123061 accctgtttg tcgccagtgg ctttgagcac tgtatcgcca atttgtttgt gattccgttc
   123121 gccattgcca ttcgccattt cgcccctacc tccttctggc aactggcgca cagtagcgca
   123181 gacaattttc cggcactgac ggtcagccat tttattaccg ccaatctgct cccggtgatg
   123241 ctgggtaata ttatcggcgg tgcagtgctg gtgagtattt gttatcgggc tatttattta
   123301 cgtcaggaat cctgaaaaat cagcccggcg aaacagttcg tcgggctgaa gattattctt
   123361 gcgcgatttg cccatcgggc caggcgtgga tcacggcttt gatcagcgtc gccaatggaa
   123421 tggcgaagaa tacgccccag aatccccaca aaccaccgaa gatcaccacc gataaaataa
   123481 tcaccagcgg atgcaggtta accgcttcgg agaacaacac cggtactagc aggttgccgt
   123541 ccagcgcctg aataatcaga tacaccgcga agcagctcca gaattcagta cctgcgccaa
   123601 actggaatag cgccacgcca accaccggaa tggtcaccac aaatgcgcca atgtacggaa
   123661 taagaaccga gaagccgacc agcactgcca gcagcagcga atagttcagc ccgaagagca
   123721 agaaccccag ccaggtggcg atccccacca cgatcatctc cagcactttg ccgcggatat
   123781 agttggtgat ttgttgattc atctccttcc acacctgtcc tgccagccca cggttacgcg
   123841 gcagcacccg gcgaacggcg ttcagcatct gctctttgtc tttcagcagg aagaagacca
   123901 tcaatggcac cagcaccaga tagacggcta tggtcagcaa accgaccagc gaggcgaggg
   123961 aaattttcac caccgaatcg cccatggtca gcatccgact gcgcatattt tcggccattg
   124021 catcaataat gcccgcgtcc attaacaccg gatagcggcg cggcaacgtg gcggcaaagt
   124081 cagaaagctt attgagcatc cccggcatat cgcggattaa gtagatgccc tgttgccagg
   124141 cgataggcag taccacgaac gccatcagta gcaatatacc gacaaaaacc accaatacaa
   124201 tcgacgtcgc ccagcggcgg gagcagccaa tagattgcag gcgcacggtt ggccattcca
   124261 gcaaataagc cagcacaata gccaccagca acggagcaag caggccacta aagaaaaaga
   124321 taatgccaaa tccggcaact aaaataacca gcaaggcaat cgcttccggg tcgctaaaac
   124381 ggcggcgata ccattgcatc aacatttcga gcataaagac cttccctgaa cctcaagagc
   124441 gggattgcga tccgcaattg tatcgaaatg tcacaaaaaa gacttcgctt tttatgacgg
   124501 attcaggaaa ctgaaaagtc atttgagtgg gctaatcttc gccgttacac tcaaaggcgg
   124561 cgaggtggga acgatatttc acagtatcgg tcaaatgact atcttcagaa atacaggata
   124621 gaggttatgt tcaggcagtt gaaaaaaaac ctggttgcaa ccctcattgc tgctatgacc
   124681 attggtcagg tagccccggc gtttgccgac agcgcagaca ccttgccgga tatgggaacc
   124741 tccgcaggaa gcacgctttc cattggtcag gaaatgcaga tgggcgacta ttatgtccgc
   124801 cagctacgcg gcagcgcgcc gttaattaat gacccgctgt taacgcaata tattaattcg
   124861 ctggggatgc gtctggtttc gcatgccaat tcggttaaga caccgtttca tttttttctg
   124921 atcaacaacg acgaaattaa cgcctttgct ttctttggcg gcaacgtggt gctgcactct
   124981 gccctgttcc gttattccga taacgaaagt caactggctt cagttatggc gcacgaaatc
   125041 tcccacgtca cccaacgtca cctggcgcga gcgatggaag atcaacaacg cagcgcgccg
   125101 ctgacctggg tcggcgcgtt aggttctatt ttactggcga tggccagtcc gcaggcgggg
   125161 atggcggcgc tgaccggtac actggcggga acgcgccagg ggatgatcag tttcacccag
   125221 caaaatgaac aggaagcgga ccgcattggt attcaggtgc tgcaacgctc gggattcgat
   125281 ccacaggcga tgccgacctt cctggaaaaa ttgctcgatc aggcgcgtta ctcctcgcgc
   125341 ccgccggaaa ttttattgac tcacccgttg ccggaaagtc gtctggcaga tgcccgcaac
   125401 cgtgctaatc agatgcgccc gatggtggtg cagtcgtcgg aagatttcta tctggcgaaa
   125461 gcgcgcacac tggggatgta taattccgga cgtaaccagc tcaccagtga tttgctggat
   125521 gaatgggcga aaggaaacgt tcgtcagcaa cgagcggcgc aatatggtcg tgctttacag
   125581 gcgatggaag ccaataaata cgacgaggcg cgtaaaacgc tgcaaccgtt actggcggca
   125641 gaacctggca acgcatggta tctcgatctg gctaccgata tcgatcttgg gcaaaacaaa
   125701 gccaatgagg caatcaatcg cctgaaaaat gcccgcgatt tacgcaccaa tccggtgttg
   125761 cagctcaacc tggcgaacgc ttatctgcaa ggcggtcaac cacaagaagc ggccaatatt
   125821 ctgaatcgct acacctttaa taataaagat gacagcaacg gctgggattt gctggcacag
   125881 gcggaagccg cgctaaataa ccgcgatcag gagctggctg cgcgagcaga aggttatgcg
   125941 ctcgccggac gactcgatca ggccatttcg ctgttgagta gcgccagttc gcaggtgaaa
   126001 ttaggcagcc tgcaacaagc gcgttacgat gcgcgcatcg accagttgcg ccagctgcag
   126061 aaacgcttta agccttatac caagatgtaa ttcgcattaa aggaagaaca atgaccaaac
   126121 aggtaaaaat ctaccataac ccacgctgtt caaagagccg ggaaacgctg aacctgctga
   126181 aagaaaacgg cgtggagcca gaagtggtgc tctaccttga gacacccgcc gatgcggcaa
   126241 cgctgcgcga tttgctgaaa atgctgggga tgaatagcgc ccgtgaattg atgcgccaga
   126301 aagaggatct ttataaagaa ctaaacctgg ctgacagttc gcttagcgaa gaggcgctga
   126361 ttcaggcaat ggtggataat ccgaagctga tggagcgccc gattatggtc gcgaatggca
   126421 aagcgcggat tggtcgtcca ccggagcagg tactggaaat cgtgggttaa gatgtgctcc
   126481 atcgctagtt gaagcacatt gccggatgcg acgcttgcca cgtcttatcc ggcctacgaa
   126541 tactatggat taacttcgcg taggtcaggc gttcgcgccg catccgacaa taaacacctt
   126601 atctacaact tcagaatttc tttcacaaac ggaatggtca gcttacgttg cgcggtaatc
   126661 gacgcacgat ccaactgatc caacgtcata aatagcgtgc gcatttctct gtcgagccgc
   126721 ttcagcaaga aacgccccac atcttccggc agttcaaaac cacgcaaacg cgcgcgtaac
   126781 tgtagcgcct gcaacttatc ttcatcagaa agtggctgca atttgtagat ctgcccccag
   126841 tcgagacgcg acgcgagatc cggtaatccc agattcaact gccgcggtgg acgatcgccg
   126901 gtgatcaaca accgtgtttt gcccgattcc agaattcgat tgtagagatc gaaaatcgcc
   126961 atctcccaca actcatcgcc tgcaatacac tcaatgttgt cgatacagac cagcgacaaa
   127021 tgctccatac cgtcgagcac ttccggaaca aaccaggtgc gtttatccag cgggacatag
   127081 cccaccgcat cgccacgctg cgacaattcc gcgcaagccg cgtgcagcag atggctgcgc
   127141 cccgcgcctt cgcgtgccca gagatagatg taaccgctat gttcctgacg cagcacgttt
   127201 tgcagcgcgg ccagtaaaga ggagttatcc cccggccaga aacttgcaaa ggtttcgtcg
   127261 tcaggaagat aaagtggcaa agagagctgt gccggtgtgt tcagagatac ctcaaccagg
   127321 atttcacaaa atcgcgagaa gtttaccaca gaatcccatg atgtttgaac cgggcagcaa
   127381 cactgcccgg tcggtacatt atttgtctgt tatatccgcg tcttctgcgt ccagcaccac
   127441 ttcttcaggg cgcagcacac tgatcagttt gaaaatcaaa cttaagccga taccgacgat
   127501 ggtcgccagc gccatacctt ttaactccgc cgcgccgatg tttaccttcg cgccactgac
   127561 gccgatgatc aaaatcacgg aagtcaggat caggttctgt gctttgttgt aatccacttt
   127621 cgattcgatc aaaacacgaa taccggaagc accaatgaca ccataaagca gcagcgaaac
   127681 gccgcccatc accggcaatg ggatcatctg gatagcggca gccagtttac cgacgcagga
   127741 aagcaggata gcgaaaatcg ccgccccgcc gataacccag gtactgtaaa cacgggtgat
   127801 cgccatcacg ccaatgtttt ctccgtaagt ggtatttggc gtagagccaa agaagccgga
   127861 gatcacggtc gacaggccgt tagcaaacat cgaacggtgc agacctggat cgcgcagcag
   127921 atcttttttg acgatattag ccgttactac caggtgccct acgtgttcgg caataaccac
   127981 taacgccgct ggcagaatag tcagaatggc aaaccactcg aagcgcggcg tatagagggt
   128041 tggcagtgca aaccagtgag cattaataat cggcgtggta tcgacaattc ccattgcgaa
   128101 agagagcgcg taccccacca gcacgccaat taaaatcggg ataattgcca ggaaaccacg
   128161 aaacagcacg gaacctaaaa ccgtgaccgc cagggtggta atagagatga tgatggtttt
   128221 ggagtttggc gtttgccctt cagccgggag taaacccgcc ataccggcag ctacgcccgc
   128281 cagctccaga ccgatgacgg caacgattgc gcccattgcc gcaggtggaa acagcacgtc
   128341 caaccagccg gtccccgctt tcttcacgat aaaagaaacc aggcagaaca gcacgccgca
   128401 cataataaag ccgcccagcg cgacttcata ccctaacggc aacagtaaca ataccggtga
   128461 aataaaggca aagctggaac caagataagc cggaattttc cctttacaga tgaagagata
   128521 cagcagcgtt ccaataccgt taaataacag tacagtcgcc gggttaatat gaaataagac
   128581 gggcaccagg acggttgcac caaacatggc gaacaaatgt tgcaaactaa gcgggattgt
   128641 ctgtaaaagt ggcggtcttt cactcacccc gatagcacgg cgcgtcatag tattatcctc
   128701 tgtattatgt gttataggcg ctttactcaa aaaaaagccg actcttaaag tcggctttaa
   128761 ttatttttat tctttatttc gtaccaaaga ttttgtcacc ggcatcgccg aggcccggaa
   128821 taatgtatcc gtgctcgttc agtccctggt caatcgatgc agtatacagt tcgacgtccg
   128881 ggtgcgcttt ttccagcgca gcgatacctt ctggcgcagc gaccagcacc agaactttga
   128941 tgctgctgca gcccgctttt ttcagcaggt cgatggtcgc gataacggaa ccaccggttg
   129001 ccagcattgg gtcaacgatc agcgccatac gctcatcgat gttagaaacc agtttctgga
   129061 agtacggtac cggctccagc gtttcttcat tacggtacat accgacaacg ctgatgcgcg
   129121 cgctcggaac gttttccagc acaccgtcca tcataccaag acccgcacgc agaattggca
   129181 caacggtaat tttcttacct ttgatctggt cgatttctac cgggccgttc cagccttcga
   129241 tagttacttt ttccgtttcg aggtcggcgg tcgcttcgta agtcagcagg ctacccactt
   129301 cggaagcgag ttcgcgaaag cgcttggtgc tgatatcttg ctcacgcatc agtcccagct
   129361 tgtgtttgac gagtgggtgt ttgacttcca cgatcttcat actctttctc ctttgagggg
   129421 cagccacaaa aaaatcgacg gattatacct cctttcttca gggcggcaat attattttcg
   129481 ttgactttag tcaaaatgat aacagtttga gataaagtta ttttatattc aaatggttat
   129541 gaaagaagat tattccatcc gaaaactaac ctttgccctg gcacaagtct tctttcgccg
   129601 cgcgcccggg gaaaagacgt gcaaaaaggt tgtgtaaaac agtctcgcaa acgtttgctt
   129661 tccctgttag aattgcgccg aattttattt ttctaccgca agtaacgcgt ggggacccaa
   129721 gcagtgaccg ataaaacctc tcttagctac aaagatgccg gtgttgatat tgacgcgggt
   129781 aatgctctgg ttggaagaat caaaggcgta gtgaagaaaa cgcgtcgtcc ggaagtgatg
   129841 ggcggtctgg gcggcttcgg tgcgctgtgt gcattgccgc aaaaatatcg tgaacccgtg
   129901 ctggtttccg gcactgacgg cgtaggtacc aagctgcgtc tggcaatgga cttaaaacgt
   129961 cacgacacca ttggtattga tctggtcgcc atgtgcgtta atgacctggt ggtgcaaggt
   130021 gcggaaccgc tgtttttcct cgactattac gcaaccggaa aactggatgt tgataccgct
   130081 tcagcggtga tcagcggcat cgcggaaggt tgtctgcaat cgggctgttc tctggtgggt
   130141 ggcgaaacgg cagaaatgcc ggggatgtat cacggtgaag attacgatgt cgcgggtttc
   130201 tgcgtgggcg tggtagaaaa atcagaaatc atcgacggct ctaaagtcag cgacggcgat
   130261 gtgctgattg cactcggttc cagcggtccg cactcgaacg gctattcgct ggtgcgcaaa
   130321 attcttgaag tcagcggttg tgatccgcaa accaccgaac ttgatggtaa gccattagcc
   130381 gatcatctgc tggcaccgac ccgcatttac gtgaagtcag tgctggagtt gattgaaaag
   130441 gtcgatgtgc atgccattgc gcacctgacc ggcggcggct tctgggaaaa cattccgcgc
   130501 gtattgccag ataataccca ggcagtgatt gatgaatctt cctggcagtg gccggaagtg
   130561 ttcaactggc tgcaaacggc aggtaacgtt gagcgccatg aaatgtatcg caccttcaac
   130621 tgcggcgtcg ggatgattat cgccctgcct gctccggaag tggacaaagc cctcgccctg
   130681 ctaaatgcca acggtgaaaa cgcgtggaaa atcggtatca tcaaagcctc tgattccgaa
   130741 caacgcgtgg ttatcgaata atgaatattg tggtgcttat ttccggcaac ggaagtaatt
   130801 tacaggcaat tattgacgcc tgtaaaacca acaaaattaa aggcaccgta cgggcagttt
   130861 tcagcaataa ggccgacgcg ttcggccttg aacgcgcccg ccaggcgggt attgcaacgc
   130921 atacgctcat cgccagcgcg tttgacagtc gtgaagccta tgaccgggag ttgattcatg
   130981 aaatcgacat gtacgcaccc gatgtggtcg tgctggctgg ttttatgcgc attctcagcc
   131041 cggcgtttgt ctcccactat gccgggcgtt tgctgaacat tcacccttct ctgctgccga
   131101 aatatcccgg attacacacc catcgtcagg cgctggaaaa tggcgatgaa gagcacggta
   131161 catcggtgca tttcgtcacc gatgaactgg acggtggccc ggttatttta caggcgaaag
   131221 tcccggtatt tgctggtgat acggaagatg acatcaccgc ccgcgtgcaa acccaggaac
   131281 acgctattta tccactggtg attagctggt ttgccgatgg tcgtctgaaa atgcacgaaa
   131341 acgccgcgtg gctggatggt caacgtctgc cgccgcaggg ctacgctgcc gacgagtaat
   131401 gcccccgtag ttaaagcgcc agctctgccg ctggcgtttt tcaattcacc tgtaaatcgc
   131461 aagctccagc agtttttttc ccccttttct ggcatagttg gacatctgcc aatattgctc
   131521 gccataatat ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta atgggtcagg
   131581 aaaagctata catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg
   131641 aagcggcgga caaatctaac ccgctgattg aaaggatgcg tttcctgggg atctattcca
   131701 ataaccttga tgagttctat aaagtccgct tcgctgaact gaagcgacgc atcattatta
   131761 gcgaagaaca aggctccaac gctcattccc gccatttact gggcaaaatt cagtcccggg
   131821 tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga gctattgctg gagatggcgc
   131881 gcaaccagat cttcctgatt aatgaacgcc agctctccgt caatcaacaa aactggctgc
   131941 gtcattattt taagcagtat ctgcgtcagc acattacgcc gattttaatc aatcctgaca
   132001 ctgacttagt gcagttcctg aaagatgatt acacctatct ggcggtggaa attatccgta
   132061 gcgatagcat ccgttacgcg ctgctggaga tcccatcaga taaagtgccg cgctttgtga
   132121 atttaccgcc agaagcgccg cgtcgacgca agccgatgat tcttctggat aacattctgc
   132181 gttactgcct tgatgatatt ttcaaaggct tctttgatta tgacgcgctg aatgcctatt
   132241 caatgaagat gacccgcgat gccgaatacg atttagtgca tgagatggaa gccagcctga
   132301 tggagttgat gtcttccagt ctcaagcagc gtttaactgc tgagccggtg cgttttgttt
   132361 atcagcgcga tatgcccaat gcgctggttg aagtgttacg cgaaaaactg actatttccc
   132421 gctacgactc catcgtcccc ggcggtcgtt atcataattt taaagacttt attaatttcc
   132481 ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc gcgtttacgc catatttggt
   132541 ttgataaagc ccagttccgc aatggttttg atgccattcg cgaacgcgat gtgttgctct
   132601 attatcctta tcacaccttt gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc
   132661 cgagcgtact ggcgattaaa attaacattt accgcgtggc gaaagattca cgcatcatcg
   132721 actcaatgat ccacgccgca cataacggta agaaagtcac tgtggtggtt gagttacagg
   132781 cgcgtttcga cgaagaagcc aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc
   132841 acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg atttcacgta
   132901 aagaaaacgg tgaagtggtc cgttacgcac acatcgggac cgggaacttt aacgaaaaaa
   132961 ccgcgcgtct ttatactgac tattcgttgc tgaccgcaga tgcgcgtatc accaacgaag
   133021 tacggcgggt atttaacttt attgaaaacc cataccgccc ggtgacattt gattatttaa
   133081 tggtgtcacc gcaaaactct cgccgtctgt tatatgaaat ggtagaccgc gaaatcgcca
   133141 acgcgcagca agggctgccc agtggtatca ccctgaagct aaataacctt gtcgataaag
   133201 gcctggttga tcgtctgtat gcggcctcca gctccggcgt accggttaat ctgctggttc
   133261 gcggaatgtg ttcgctgatc cccaatctgg aaggcattag cgacaacatt cgtgccatca
   133321 gtattgttga ccgttacctt gaacatgacc gggtttatat ttttgaaaat ggcggcgata
   133381 aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa tattgattat cgtattgaag
   133441 tggcgacacc gctgctcgat ccgcgcctga agcagcgggt gctggacatc atcgacatat
   133501 tgttcagcga tacggtcaaa gcacgttata tcgataaaga actcagtaat cgctacgttc
   133561 cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat ttacgactac atcaaatcac
   133621 tcgaacaacc tgaataaccc tatgccaata cacgataaat cccctcgtcc gcaggagttt
   133681 gctgcggtcg atcttggttc aaacagtttt cacatggtca tagcccgtgt ggtagatggt
   133741 gccatgcaga ttattggccg cctgaaacag cgggtgcatc tggcggacgg cctggggcca
   133801 gataatatgt tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc
   133861 gaacggctac aagggttttc tcctgccagc gtctgtatag tcggcaccca tacgctgcgt
   133921 caggcgctga acgccactga ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt
   133981 gaaattattt ccggtaatga agaagcccgt ctgattttta tgggcgtgga acatacccaa
   134041 ccggaaaaag gtcgcaaact ggttattgat attggcggcg gatctacgga actggtgatt
   134101 ggtgaaaatt tcgaacctat tctcgttgaa agccgccgga tgggttgtgt cagctttgcc
   134161 cagctttatt ttcctggcgg ggtcatcaat aaagagaatt ttcagcgcgc tcgcatggcg
   134221 gcagcacaaa aactggaaac tttaacctgg caattccgta ttcagggctg gaacgttgca
   134281 atgggcgctt ccggtaccat aaaagccgcc catgaagtgt taatggaaat gggcgagaaa
   134341 gacgggataa ttaccccgga acgtctggaa aaactggtaa aagaaatttt acgtcaccgt
   134401 aatttcgcat cgctgagttt accgggtctt tccgaagagc ggaaaacagt cttcgttcct
   134461 ggactggcga ttttatgtgg tgtgtttgat gctttagcca tccgtgaact gcgcctttct
   134521 gacggggcgt tacgcgaagg cgtactgtat gaaatggaag gacgtttccg tcatcaggat
   134581 gtgcgtagtc gcaccgccag cagcctcgcc aaccagtatc acatagacag cgaacaggcc
   134641 cgacgagtgc tggataccac tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag
   134701 ctggcgcatc cgcaactgga ggcgctactg cgatgggccg ccatgctgca tgaggtcggg
   134761 ttgaatatca accacagcgg tttgcatcgc cactccgctt atattctgca aaacagtgac
   134821 ttgccgggtt ttaatcagga acagcagctg atgatggcga cactggtgcg ctatcaccgt
   134881 aaagcgatta agctcgacga tctgccgcgc tttaccttgt ttaagaagaa acagttcctg
   134941 ccactgatac agctattgcg ccttggcgta ttactaaaca atcaacgtca ggcaaccacc
   135001 acaccgccaa cattgacact gattactgat gacagtcact ggacactgcg tttcccgcat
   135061 gactggttta gtcagaatgc gctggtactg cttgatctgg aaaaggagca agaatactgg
   135121 gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa gtaccccaga aatcgccgct
   135181 taatgctttg cgggccgacg agaaatgtcg gcccgcatta ttcaggcact ttcgcgaatg
   135241 ggttcgattt cattcagcgt atcaattaac ggttgcggct taccaataag ataaccctgc
   135301 atataatcga tccccaaaga gagcaccgcc tcgcggatct cttcgttttc aacgtactct
   135361 gccactaccc gcattttctt cattcgcgcc agatggcaaa tcgacgccac aatctgataa
   135421 tccagactat ttgacacaat attgcggata aagctgccgt caattttaag cagatcggca
   135481 ttcacatttt taagccgtgc atagctggcg tagccggtgc cgaaatcatc aatcgcaatc
   135541 tggcagccta attcctgaag atgctgcaag gtgatttgcg cctgcttaac attggtcaga
   135601 gcattacttt cggtgacttc aaaaataagt tgccacgctt caatctgata tttagccagc
   135661 aactgactga cttcaacagg aaaacgagcc tgacataccg aggttggaga cagattaata
   135721 gcaaaacggt gagcgggcat cttcgctctg ttttcagcca taaattgcag cgtatgctca
   135781 atgacccaca tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg caagaagcta
   135841 ccggggccga tcagttcatc attctcacct ttcatgcgca gaagaatttc atggtaaaca
   135901 tcaccacgca taccggtaat cggctgggcc atcaggaaaa aatggttgtg ttccagcgcc
   135961 tgctgtagtc gattcatcat cgcgacttta tctttcaatt cgcgttgcaa atacattgcc
   136021 ccgcaacgct gcatattttc cggggcgttg gtcacgatgg aaagttcggc gaccgtattt
   136081 agctctccca gcagcaggta gatatgattc actggcgagc gcacatagca gtaactgacg
   136141 ccaatctgcg gttgcatcgg catcccatcc caaaagaaac gaaattgctt gagatgacta
   136201 tccagtgcgg taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta
   136261 cccgaaagct gataaacatc ttcacccggt tccagcaagg gtga
//
LOCUS       DAJTRM010000002        95385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRM010000002 DAJTRM010000000
VERSION     DAJTRM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.002"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO322_000132"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO322_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5905153.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO322_000133"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO322_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5905154.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO322_000134"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO322_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5905155.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO322_000135"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO322_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5905156.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO322_000136"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO322_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5905157.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO322_000137"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO322_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5905158.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO322_000138"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO322_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5905159.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO322_000139"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO322_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5905160.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO322_000140"
     CDS             complement(8942..9223)
                     /locus_tag="OO322_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905161.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO322_000141"
     CDS             complement(9237..10898)
                     /locus_tag="OO322_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5905162.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO322_000142"
     CDS             complement(10882..11208)
                     /locus_tag="OO322_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5905163.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO322_000143"
     CDS             complement(11264..11446)
                     /locus_tag="OO322_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905164.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO322_000144"
     CDS             complement(11430..11870)
                     /locus_tag="OO322_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5905165.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO322_000145"
     CDS             complement(11870..12166)
                     /locus_tag="OO322_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5905166.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO322_000146"
     CDS             complement(12163..12501)
                     /locus_tag="OO322_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5905167.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO322_000147"
     CDS             complement(12498..13709)
                     /locus_tag="OO322_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5905168.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO322_000148"
     CDS             complement(13711..14283)
                     /locus_tag="OO322_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5905169.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO322_000149"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO322_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OO322_000150"
     CDS             complement(15781..16005)
                     /locus_tag="OO322_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905170.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OO322_000151"
     CDS             complement(16147..16308)
                     /locus_tag="OO322_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5905171.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OO322_000152"
     CDS             complement(16318..16623)
                     /locus_tag="OO322_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905172.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OO322_000153"
     CDS             complement(16620..16898)
                     /locus_tag="OO322_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905173.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OO322_000154"
     CDS             complement(17187..18935)
                     /locus_tag="OO322_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5905174.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OO322_000155"
     CDS             complement(18932..19231)
                     /locus_tag="OO322_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5905175.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OO322_000156"
     CDS             complement(19237..19470)
                     /locus_tag="OO322_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905176.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OO322_000157"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OO322_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OO322_000158"
     CDS             complement(19685..19906)
                     /locus_tag="OO322_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905177.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OO322_000159"
     CDS             complement(19896..20096)
                     /locus_tag="OO322_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5905178.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OO322_000160"
     CDS             20226..20405
                     /locus_tag="OO322_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905179.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OO322_000161"
     CDS             20530..20859
                     /locus_tag="OO322_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905180.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OO322_000162"
     CDS             complement(20909..21097)
                     /locus_tag="OO322_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5905181.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OO322_000163"
     CDS             complement(21472..22701)
                     /locus_tag="OO322_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5905182.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="OO322_000164"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="OO322_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5905183.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO322_000165"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO322_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5905184.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="OO322_000166"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="OO322_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5905185.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="OO322_000167"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="OO322_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5905186.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="OO322_000168"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="OO322_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5905187.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="OO322_000169"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="OO322_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5905188.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO322_000170"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO322_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5905189.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO322_000171"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO322_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5905190.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO322_000172"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO322_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5905191.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO322_000173"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO322_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5905192.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO322_000174"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO322_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5905193.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="OO322_000175"
     CDS             34739..35812
                     /locus_tag="OO322_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5905194.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="OO322_000176"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="OO322_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5905195.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="OO322_000177"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="OO322_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5905196.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="OO322_000178"
     CDS             complement(40574..40786)
                     /locus_tag="OO322_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5905197.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="OO322_000179"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="OO322_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5905198.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="OO322_000180"
     CDS             complement(41765..42304)
                     /locus_tag="OO322_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5905199.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO322_000181"
     CDS             complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO322_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5905200.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="OO322_000182"
     CDS             complement(44243..44785)
                     /locus_tag="OO322_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5905201.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO322_000183"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO322_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5905202.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO322_000184"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO322_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5905203.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO322_000185"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO322_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5905204.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47682)
                     /locus_tag="OO322_000186"
     CDS             complement(47305..47682)
                     /locus_tag="OO322_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5905205.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO322_000187"
     CDS             complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO322_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5905206.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48377..50562
                     /gene="fhuE"
                     /locus_tag="OO322_000188"
                     /pseudo
     CDS             48377..50562
                     /gene="fhuE"
                     /locus_tag="OO322_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OO322_000189"
     CDS             complement(50622..52055)
                     /gene="ptsG"
                     /locus_tag="OO322_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5905207.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52350..53147)
                     /locus_tag="OO322_000190"
     CDS             complement(52350..53147)
                     /locus_tag="OO322_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5905208.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OO322_000191"
     CDS             complement(53158..54162)
                     /gene="holB"
                     /locus_tag="OO322_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5905209.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54159..54800)
                     /locus_tag="OO322_000192"
     CDS             complement(54159..54800)
                     /locus_tag="OO322_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5905210.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OO322_000193"
     CDS             complement(54790..55812)
                     /gene="yceG"
                     /locus_tag="OO322_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5905211.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OO322_000194"
     CDS             complement(55815..56624)
                     /gene="pabC"
                     /locus_tag="OO322_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5905212.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OO322_000195"
     CDS             complement(56744..57985)
                     /gene="fabF"
                     /locus_tag="OO322_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5905213.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OO322_000196"
     CDS             complement(58072..58308)
                     /gene="acpP"
                     /locus_tag="OO322_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5905214.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OO322_000197"
     CDS             complement(58519..59253)
                     /gene="fabG"
                     /locus_tag="OO322_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5905215.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OO322_000198"
     CDS             complement(59266..60195)
                     /gene="fabD"
                     /locus_tag="OO322_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5905216.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OO322_000199"
     CDS             complement(60211..61164)
                     /gene="fabH"
                     /locus_tag="OO322_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5905217.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OO322_000200"
     CDS             complement(61232..62302)
                     /gene="plsX"
                     /locus_tag="OO322_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5905218.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OO322_000201"
     CDS             complement(62383..62556)
                     /gene="rpmF"
                     /locus_tag="OO322_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5905219.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OO322_000202"
     CDS             complement(62608..63129)
                     /gene="yceD"
                     /locus_tag="OO322_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5905220.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63328..63912
                     /gene="yceF"
                     /locus_tag="OO322_000203"
     CDS             63328..63912
                     /gene="yceF"
                     /locus_tag="OO322_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5905221.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OO322_000204"
     CDS             complement(64024..64983)
                     /gene="rluC"
                     /locus_tag="OO322_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5905222.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65556..68747
                     /gene="rne"
                     /locus_tag="OO322_000205"
     CDS             65556..68747
                     /gene="rne"
                     /locus_tag="OO322_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5905223.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OO322_000206"
                     /pseudo
     CDS             complement(68944..69886)
                     /gene="flgL"
                     /locus_tag="OO322_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OO322_000207"
                     /pseudo
     CDS             complement(69898..71540)
                     /gene="flgK"
                     /locus_tag="OO322_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OO322_000208"
     CDS             complement(71606..72547)
                     /gene="flgJ"
                     /locus_tag="OO322_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5905224.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OO322_000209"
     CDS             complement(72547..73644)
                     /gene="flgI"
                     /locus_tag="OO322_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5905225.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OO322_000210"
     CDS             complement(73656..74354)
                     /gene="flgH"
                     /locus_tag="OO322_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5905226.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OO322_000211"
     CDS             complement(74407..75189)
                     /gene="flgG"
                     /locus_tag="OO322_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5905227.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OO322_000212"
                     /pseudo
     CDS             complement(75327..76078)
                     /gene="flgF"
                     /locus_tag="OO322_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76098..77237)
                     /locus_tag="OO322_000213"
     CDS             complement(76098..77237)
                     /locus_tag="OO322_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5905228.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OO322_000214"
     CDS             complement(77262..77957)
                     /gene="flgD"
                     /locus_tag="OO322_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5905229.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OO322_000215"
                     /pseudo
     CDS             complement(77969..78372)
                     /gene="flgC"
                     /locus_tag="OO322_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OO322_000216"
     CDS             complement(78376..78792)
                     /gene="flgB"
                     /locus_tag="OO322_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5905230.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78947..79606
                     /gene="flgA"
                     /locus_tag="OO322_000217"
     CDS             78947..79606
                     /gene="flgA"
                     /locus_tag="OO322_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5905231.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79682..79975
                     /gene="flgM"
                     /locus_tag="OO322_000218"
     CDS             79682..79975
                     /gene="flgM"
                     /locus_tag="OO322_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5905232.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79980..80396
                     /gene="flgN"
                     /locus_tag="OO322_000219"
     CDS             79980..80396
                     /gene="flgN"
                     /locus_tag="OO322_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5905233.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OO322_000220"
     CDS             complement(80436..81971)
                     /gene="murJ"
                     /locus_tag="OO322_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5905234.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82171..83004)
                     /locus_tag="OO322_000221"
     CDS             complement(82171..83004)
                     /locus_tag="OO322_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5905235.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83006..83653)
                     /locus_tag="OO322_000222"
     CDS             complement(83006..83653)
                     /locus_tag="OO322_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5905236.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OO322_000223"
     CDS             complement(83664..84248)
                     /gene="rimJ"
                     /locus_tag="OO322_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5905237.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84484..85692
                     /gene="mdtH"
                     /locus_tag="OO322_000224"
     CDS             84484..85692
                     /gene="mdtH"
                     /locus_tag="OO322_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5905238.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85756..86403
                     /gene="grxB"
                     /locus_tag="OO322_000225"
     CDS             85756..86403
                     /gene="grxB"
                     /locus_tag="OO322_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5905239.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86537..87097
                     /locus_tag="OO322_000226"
     CDS             86537..87097
                     /locus_tag="OO322_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5905240.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87203..88249
                     /gene="pyrC"
                     /locus_tag="OO322_000227"
     CDS             87203..88249
                     /gene="pyrC"
                     /locus_tag="OO322_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5905241.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88323..88568
                     /gene="dinI"
                     /locus_tag="OO322_000228"
     CDS             88323..88568
                     /gene="dinI"
                     /locus_tag="OO322_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5905242.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88858..89112
                     /gene="bssS"
                     /locus_tag="OO322_000229"
     CDS             88858..89112
                     /gene="bssS"
                     /locus_tag="OO322_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5905243.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89227..90345
                     /gene="solA"
                     /locus_tag="OO322_000230"
     CDS             89227..90345
                     /gene="solA"
                     /locus_tag="OO322_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5905244.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90393..90506
                     /locus_tag="OO322_000231"
     CDS             90393..90506
                     /locus_tag="OO322_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5905245.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90767..91333
                     /locus_tag="OO322_000232"
     CDS             90767..91333
                     /locus_tag="OO322_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5905246.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91337..91912
                     /locus_tag="OO322_000233"
     CDS             91337..91912
                     /locus_tag="OO322_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5905247.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91954..93006)
                     /locus_tag="OO322_000234"
     CDS             complement(91954..93006)
                     /locus_tag="OO322_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5905248.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93231..94151
                     /gene="lpxL"
                     /locus_tag="OO322_000235"
     CDS             93231..94151
                     /gene="lpxL"
                     /locus_tag="OO322_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5905249.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94323..94520
                     /locus_tag="OO322_000236"
     CDS             94323..94520
                     /locus_tag="OO322_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5905250.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94544..>95385
                     /locus_tag="OO322_000237"
     CDS             94544..>95385
                     /locus_tag="OO322_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5905251.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNT"
BASE COUNT        22951 a        25285 c        23531 g        23618 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gtggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    47461 aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag gttgaatatc
    47521 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    47581 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    47641 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    47701 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    47761 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    47821 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    47881 gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct
    47941 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    48001 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    48061 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    48121 agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg
    48181 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    48241 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc agcaaaatta
    48301 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
    48361 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagtcat
    48421 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
    48481 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
    48541 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
    48601 acatgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
    48661 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
    48721 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
    48781 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactgtt
    48841 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
    48901 tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag gcgatgtctc
    48961 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
    49021 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
    49081 ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
    49141 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
    49201 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag
    49261 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
    49321 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
    49381 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
    49441 gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt ggaacagtgg
    49501 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
    49561 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt cagttcatgg
    49621 gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa
    49681 gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa atcgttatat
    49741 gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc
    49801 aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct
    49861 ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc
    49921 cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac
    49981 tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc
    50041 ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac
    50101 ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc
    50161 aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta tcttgcagag
    50221 gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc
    50281 agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat
    50341 cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac
    50401 gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac
    50461 gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat cgtctacggc
    50521 gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag
    50581 gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac
    50641 tcatccatct cggttttcag gttatcggat ttagtaccga aaatagcctg aacaccagaa
    50701 ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac
    50761 acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct
    50821 ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt tgcttttgca
    50881 tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg
    50941 aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag ccacagtttg
    51001 ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca
    51061 cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg
    51121 tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat accggtcagg
    51181 aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta
    51241 gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca
    51301 ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa
    51361 acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg
    51421 tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact
    51481 accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc
    51541 caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca
    51601 aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta
    51661 aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt atccgccaag
    51721 tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg
    51781 gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg
    51841 gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg
    51901 cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg
    51961 acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag cgatttaccg
    52021 accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg
    52081 tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc
    52141 taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct
    52201 atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca
    52261 atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc
    52321 cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg
    52381 atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca
    52441 cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt
    52501 cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa tcgatccagg
    52561 gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac aatgccggaa
    52621 aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa
    52681 cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg
    52741 gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata
    52801 tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata ataatccagc
    52861 ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca
    52921 tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt
    52981 tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa
    53041 aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat
    53101 tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta
    53161 aagatgagga accggtaaca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag
    53221 atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg
    53281 gcaaacatcc cccagtatag cctgcaggca cgagggagaa agatggtttg ccagttcggc
    53341 gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc gttttagcgc
    53401 atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc
    53461 cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt
    53521 ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc
    53581 gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca
    53641 agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac gactacgtaa
    53701 tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg
    53761 ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt
    53821 tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt cggtgacctc
    53881 acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgtagtaatc
    53941 gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc
    54001 ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat
    54061 gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac
    54121 cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc
    54181 cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca
    54241 atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta
    54301 aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt
    54361 aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa atccccgaga
    54421 acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc gccctgatac
    54481 gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc cagcgcaggt
    54541 ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta
    54601 tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg
    54661 gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg
    54721 agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg
    54781 acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt
    54841 gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata
    54901 gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtagc
    54961 tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag
    55021 gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggttgggtc
    55081 ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg
    55141 ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca gctgattttt
    55201 atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat
    55261 tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat acatcaaggt
    55321 gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac
    55381 ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac gcaattgctt
    55441 gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg cttctttacc
    55501 gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc
    55561 ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa cccgtgggcg
    55621 attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg tccctggctt
    55681 cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt
    55741 ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga taatcaataa
    55801 cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt
    55861 gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat taacgcatta
    55921 caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac ttcgacaagc
    55981 tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc gttaacacct
    56041 gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa attagccgca
    56101 cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc
    56161 tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat acctgcaaga
    56221 tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc gttacgcaaa
    56281 cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg tccgctgttc
    56341 aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag cacaccattt
    56401 tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa gtcacaggaa
    56461 atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa caaactgact
    56521 ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg
    56581 cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc tccttatcag
    56641 agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa
    56701 aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc tttttaaaga
    56761 tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc
    56821 tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca
    56881 ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagattcta
    56941 ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac
    57001 ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta tcgccagccg
    57061 gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac
    57121 gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat
    57181 gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt
    57241 ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaagc
    57301 catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata
    57361 atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc tctgcgccac
    57421 ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt
    57481 gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg ccatacatga
    57541 tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct
    57601 tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa
    57661 tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag
    57721 aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc
    57781 gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt
    57841 tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa
    57901 gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg
    57961 tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat gattctagtg
    58021 ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg
    58081 gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc
    58141 cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga
    58201 atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc
    58261 cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt
    58321 cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca
    58381 actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata
    58441 acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc
    58501 attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc
    58561 cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc
    58621 gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag
    58681 cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg
    58741 tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc
    58801 gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg
    58861 accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga
    58921 aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc
    58981 acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt
    59041 ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt
    59101 ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag tgccaataac
    59161 tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc
    59221 ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcgagcgc
    59281 cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt
    59341 cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta cgccttgcgc
    59401 tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc
    59461 gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca caacagaaac
    59521 tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt
    59581 catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt ttgcgccagc
    59641 agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa taaccacctg
    59701 tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt cttcacacgc
    59761 tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg tgccttccgg
    59821 tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc
    59881 aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg ccatcattgc
    59941 cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg cagtcaacag
    60001 cgctggctgg gtttgccagg ttttattcag ttcttcagct gacccctgct gggtcagcgc
    60061 ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata
    60121 gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa
    60181 tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag agccccaagt
    60241 gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg
    60301 tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag
    60361 cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac tgataatacg
    60421 caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tattcgccgc
    60481 cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc
    60541 cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg gcagcgtcag
    60601 caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg gctcttcaga
    60661 ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatctgt
    60721 tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc
    60781 agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac aggctgctgc
    60841 aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac aagctgcgct
    60901 cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc
    60961 aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc
    61021 gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga
    61081 ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc
    61141 agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact
    61201 tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt
    61261 ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa
    61321 cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg
    61381 ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact
    61441 ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata
    61501 acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca
    61561 tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg tcacaaacca
    61621 gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag
    61681 aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc tttacttctt
    61741 cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca
    61801 gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg acgttggccc
    61861 ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc accagcgccg
    61921 gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg
    61981 tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc agggccatac
    62041 gcattgaact cccacggctg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa
    62101 ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta
    62161 atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca
    62221 gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta
    62281 acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc
    62341 ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt
    62401 gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt
    62461 tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg
    62521 tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt
    62581 tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa
    62641 tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg cttcggacac
    62701 ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc
    62761 aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc
    62821 cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac
    62881 ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc
    62941 gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc
    63001 actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc
    63061 ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac
    63121 cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc
    63181 ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc
    63241 agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc
    63301 aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca
    63361 tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc acctgaggtc
    63421 gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa
    63481 aaatcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta
    63541 tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa
    63601 ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg
    63661 gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg tcacctaagc
    63721 gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc gggtagcttt
    63781 aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga ccctaacacg
    63841 ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg
    63901 ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat cggtgcaggc
    63961 ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc ttatatgctt
    64021 gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc
    64081 ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag
    64141 gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc
    64201 gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg
    64261 agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac
    64321 tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct
    64381 ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact gccactgacc
    64441 gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct catgcagaga
    64501 acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc ggtcgagacg
    64561 atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc
    64621 gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat
    64681 atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga
    64741 aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct catcacccgc
    64801 ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac ctttacgcaa
    64861 aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat
    64921 acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt
    64981 catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttatt
    65041 tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa
    65101 tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt
    65161 gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac
    65221 aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa
    65281 gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg
    65341 aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca
    65401 tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc
    65461 gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc
    65521 ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa
    65581 ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata
    65641 tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca
    65701 ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc
    65761 cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca
    65821 acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc
    65881 gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc
    65941 tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc gatgaccgta
    66001 ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc
    66061 gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgcctga
    66121 aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc
    66181 aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat atcggcgaaa
    66241 tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct gcattaggtc
    66301 gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact
    66361 accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccatccggtg
    66421 gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac tccgcacgcg
    66481 cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa gccgccgatg
    66541 agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg
    66601 acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc
    66661 aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc
    66721 gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg
    66781 gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ttgatcgaag
    66841 aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt
    66901 cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt caggacggtg
    66961 ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgttgcgcg
    67021 tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg catgaagaag
    67081 cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcac
    67141 tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa cctgccgcga
    67201 ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct gcacaacctg
    67261 ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaagcca
    67321 aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc
    67381 gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac
    67441 gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc
    67501 aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca
    67561 ccgacgagca gcaagtgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc
    67621 aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac
    67681 aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag
    67741 tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg
    67801 ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc
    67861 cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc
    67921 gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg
    67981 gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga
    68041 ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg
    68101 tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc
    68161 cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcgccagttg
    68221 ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg caaccggaag
    68281 tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag ccgcaggtga
    68341 ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa ccgatggttg
    68401 aaccgcagga agagacggca gatgcagata ttgaagaagt tgccgaaact gctgaggttg
    68461 tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag
    68521 cagaagttga aacggtggcc gcggttgaac cagagatcac cgttgagcat aaccacgcta
    68581 ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg
    68641 actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa
    68701 cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa
    68761 gtaatcaagc cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa
    68821 aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc
    68881 cccggcgatc ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg
    68941 agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga
    69001 tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc
    69061 aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag
    69121 cgactccagt ttgttcagct gcgtgcctaa ttccgcgcga acggtaagga cattattcag
    69181 cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc
    69241 ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag
    69301 attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa
    69361 aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat
    69421 actttctgtt ccgccaacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt
    69481 atatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc
    69541 cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt
    69601 agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt
    69661 ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct
    69721 gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga
    69781 ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga
    69841 tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta
    69901 gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc
    69961 caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga
    70021 aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt
    70081 tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa
    70141 ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt
    70201 gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt
    70261 gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt
    70321 aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc
    70381 atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt
    70441 atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc
    70501 gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa
    70561 gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc
    70621 ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga
    70681 acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat
    70741 attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg
    70801 aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg
    70861 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
    70921 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
    70981 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
    71041 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
    71101 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgccgcc
    71161 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
    71221 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
    71281 gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgtatca
    71341 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
    71401 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
    71461 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
    71521 ttattgatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
    71581 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt
    71641 tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat
    71701 agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt tccgcactcg
    71761 cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca
    71821 atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt
    71881 tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca gaggctttga
    71941 caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
    72001 cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
    72061 ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc ggcagcgaat
    72121 catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg ttttgataac
    72181 gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
    72241 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
    72301 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
    72361 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
    72421 tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg ctcgctttcg
    72481 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
    72541 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
    72601 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt
    72661 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
    72721 cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct ggctgacatt
    72781 ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
    72841 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
    72901 cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct gggaactgtt
    72961 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
    73021 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
    73081 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
    73141 accattggtg atccgtccac cgttcagttg gttaacctga acactgctac cgcctgcgga
    73201 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
    73261 gcccttaagc ggagtcatca acaacgtacc tccacgcaag cttttggcat tacccatgga
    73321 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
    73381 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
    73441 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
    73501 agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc ttaccccctg
    73561 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
    73621 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
    73681 aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca
    73741 atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga
    73801 ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca
    73861 cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg
    73921 ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct
    73981 tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag
    74041 ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg
    74101 ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac
    74161 agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg
    74221 ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt
    74281 atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca
    74341 gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt
    74401 tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact
    74461 gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac
    74521 gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct
    74581 ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag
    74641 tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg
    74701 gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc
    74761 cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg
    74821 atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca acattacctg
    74881 gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg acagattgcc
    74941 ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga
    75001 gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag
    75061 atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt
    75121 ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga
    75181 actgatcata taatgtcctg tagtggattg ctgacaagca gagcagaagc acgcgacagt
    75241 gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt
    75301 ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct
    75361 gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca
    75421 atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc
    75481 acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt
    75541 aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt caccagtttc
    75601 agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg
    75661 ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc cgcctcgcct
    75721 atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta
    75781 cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa
    75841 cgtccagcgg gcgagaggtg taatccatct tgccgggcgt catgtctgca cccggtgttg
    75901 acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg
    75961 cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg
    76021 cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg
    76081 agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt
    76141 ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt gaccagttct
    76201 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagagtgcc aaagtttccc
    76261 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    76321 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    76381 tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat ttgataactc
    76441 accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt agcgccggtt
    76501 gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa ttccagtgtt
    76561 gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac ctgccagtta
    76621 ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg actgtcgaaa
    76681 acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa tggcgtaaca
    76741 gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt ggtagttttc
    76801 gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    76861 ggcggcgtac cggttaccgg gtaacccgtc agctgtaaac cttgcgtatt caacaggtta
    76921 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct
    76981 accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg
    77041 gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag tcccactttc
    77101 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag
    77161 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    77221 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    77281 tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct gttgccgcgg
    77341 atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta
    77401 ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc agtcaacgaa
    77461 ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat ggtgcgcaca
    77521 accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg ttgcagctca
    77581 acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc cgcaagaaca
    77641 gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg
    77701 ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat cccgctgacc
    77761 gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    77821 tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    77881 ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg atcggtggtg
    77941 gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca
    78001 tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac gccgacatgg
    78061 tgttaaccat ctccccgacg acatcaacgt tcggcatttt tatgtagccc tttgcatctg
    78121 ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct taacatcggc
    78181 aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata ccacctgttt
    78241 tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca gattactggc
    78301 cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat caaaaatatt
    78361 cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt tgatttgccc
    78421 gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact gggtgcgttc
    78481 gcgatccata tcgacggtat taccgtcaag cgaaggctgg tccggaatac ggtattgcag
    78541 ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgc tgcgttgagg tcatcgtcag
    78601 tgcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa
    78661 atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg ctgccagcac
    78721 ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag
    78781 cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta
    78841 cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca
    78901 ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa
    78961 acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga gcaatctcac
    79021 atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt
    79081 ttctattcgt acagcgccca atctactacc gccatgcgag cagccattgc tttcgatgag
    79141 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    79201 atatttacag gttaatgtgc aggccacagg aaattatgtg gttgccgcga tgcctattgt
    79261 gcggggagga aagctggaag ctggcaatgt caaactgaaa cgtggacgcc tggataccct
    79321 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc tgcgcgatct
    79381 atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg taaaagcggg
    79441 acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    79501 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg aacaggtagt
    79561 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    79621 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    79681 catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac
    79741 caccgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca ccagcaccag
    79801 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    79861 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacaccgg
    79921 caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta actgatcgta
    79981 tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    80041 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    80101 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    80161 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    80221 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    80281 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    80341 gggcgaacgg tcagacctca acaccccatc gcggcagtaa aaagatttcg atctgaatat
    80401 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    80461 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    80521 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    80581 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    80641 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    80701 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    80761 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    80821 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    80881 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    80941 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    81001 gcaaccgcac tcggcagtgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    81061 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag caaaatggtg
    81121 ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc gtaatacatc
    81181 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    81241 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctcca
    81301 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    81361 tgatacacca gctgcagaat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    81421 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    81481 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    81541 gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    81601 aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    81661 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    81721 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    81781 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    81841 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    81901 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    81961 aataaattca tcggtgttct aatccggacc cacgggcaaa acgcctgcaa ggcaggcgct
    82021 gaaagaggcg aatagtctac ctagattatg gtgaattgct accgccagat gttacagggt
    82081 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcactgccta
    82141 ttcgccggcg gtttgcggaa ctgtctgatt ttacacgcat tcaatgaagt gccgcgcaca
    82201 gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt tattcactac
    82261 accctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga gcgcaccgtc
    82321 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aagtggtgat
    82381 ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    82441 cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg cggtatccac
    82501 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cgctattgct
    82561 acgatgttta tccattctta gcgaggctgc ggtggcgagt tgcgttttta actcaccgta
    82621 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    82681 ttccaccagc ctttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    82741 tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggagtgcac
    82801 aaaaaccgca tcacaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    82861 gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac caggctcctt gtaacgtcca
    82921 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc ctaatcccac
    82981 tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    83041 gttgtttcag ttctgccact tcgatttcca gggcttcgac gcgggtctgt aaatcaccgt
    83101 caaccatgtt cgacatagcc gttaccgccg gctgatcttc aacctcacca ctgaaaagat
    83161 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    83221 cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    83281 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacaacg
    83341 tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct gaattacaaa
    83401 aacgttgctc atatttggtg acccgattac caaaaccgct cactgtgcgt agataatgac
    83461 gtttgaccag attatccagc tgttcctgca cttcggattc actcagattc atgaccggtt
    83521 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc gggtattgct
    83581 ccggcgtggt cacctgcttt tccagcaagc aaccaattac gcgagcttcc agggcagtaa
    83641 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    83701 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    83761 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    83821 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    83881 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    83941 tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    84001 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatccct
    84061 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    84121 ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc acgatcatgc
    84181 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    84241 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    84301 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    84361 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatactgtcc ccgaagtgat
    84421 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    84481 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    84541 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    84601 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    84661 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    84721 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    84781 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    84841 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    84901 ttgatgatgc aggacagtgc cagtgcggtc attggcgcat tgttggggag ctggctgttg
    84961 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct atgtgcggcg
    85021 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    85081 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    85141 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    85201 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    85261 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttgatggct
    85321 gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg cctacaacaa
    85381 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    85441 accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg gtttagccgt
    85501 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct gtttgacctg
    85561 ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggaattat tggcatcttc
    85621 actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa
    85681 cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagtga actgacagcc
    85741 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    85801 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    85861 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    85921 agccgctata tgccagaaag catggatatc gttcactatg tcgataaact cgacggcaaa
    85981 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    86041 tacgtcaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    86101 cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    86161 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg tgcgctggac
    86221 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    86281 tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag ccgcgttgct
    86341 aattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    86401 taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    86461 gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg acgcagattt
    86521 catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg tcagtggcct
    86581 gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa ttaaccagtc
    86641 gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg ctgacgccca
    86701 tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata aggttaccct
    86761 aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag cgaccatgaa
    86821 actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga
    86881 gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt
    86941 gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg cttacgtcct
    87001 gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt
    87061 gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca ttcgcgccct
    87121 ttatttttcg tgcaaaggaa aacgtttccg cttatccttt gtgtccggca aaaacatccc
    87181 ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga tccgccgccc
    87241 agacgactgg caccttcacc tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac
    87301 cagcgaaatt tatggacggg ctatcgtaat gcccaatctg gctccgcccg tgaccacagt
    87361 tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc gtacctgccg ggcacgattt
    87421 caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc tggagcgcgg
    87481 atttaatgaa gccgtgttca ccgctgcaaa actttacccg gcaaacgcaa ccactaactc
    87541 cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca tggaaaaaat
    87601 cggtatgccg ctactggtgc atggtgaagt aacacatgca gatatcgaca tttttgatcg
    87661 tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa
    87721 agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg acggaaatga
    87781 acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc atatgctggt
    87841 tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata ttcaccaaca
    87901 ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta cggattctgc
    87961 gccacatgca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct tcaacgcccc
    88021 aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc agcactttga
    88081 agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg acacattcat
    88141 cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg atgacaccct
    88201 ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa aatcgtagct
    88261 tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa cagggggcta
    88321 ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg gctattgacg
    88381 ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat gaaggccacg
    88441 tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca aaagaagata
    88501 aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca
    88561 gcgaataata tgcagtgatt tttgtttttg ccggatcgcc ccggcttttt tattttactt
    88621 acgaattttg agtacaaaat ttctacactt cctcaaaata attgcacaat atgctaaaaa
    88681 aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa atttatctgt
    88741 tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag ttgcttcaga
    88801 taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg ggccatgatg
    88861 gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat cagcaccgtt
    88921 gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa gtccgagcag
    88981 gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag acaatttatt
    89041 tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt aaacgagtat
    89101 cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt gcctttacca
    89161 tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga gagcagagag
    89221 aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc tgccgccggg
    89281 tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat gccaccgcat
    89341 caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa
    89401 aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg atgaactttc ccgccacaac
    89461 gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc tgactccgca
    89521 tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga aaagctcgat
    89581 gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta catcggctta
    89641 tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg gatccaactg
    89701 gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat tcgtcatgac
    89761 gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa agcgattgtc
    89821 tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc cgtacgtaaa
    89881 gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt cccggcgttt
    89941 accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa cgacgcgttg
    90001 aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg tgttccgttt
    90061 gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt
    90121 atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga cgaagatttt
    90181 attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct gagtgggcac
    90241 ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca agaccaaaaa
    90301 agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc attacggcct
    90361 ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac tatctcatca
    90421 ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg gcaattatgg
    90481 atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac aaatatttaa
    90541 tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta cagcactcac
    90601 cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta ttaattcaaa
    90661 attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc
    90721 cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt cattcacaaa
    90781 tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt
    90841 ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta
    90901 tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat
    90961 tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt attcgcccat
    91021 gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt tcgccatcgg
    91081 catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt
    91141 tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg gtgccctgca
    91201 tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta tggccctgaa
    91261 gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga
    91321 ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt ccctgatgtt
    91381 ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt
    91441 tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct ttaaagattt
    91501 cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga atgtgacaat
    91561 taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc gcagtgcgga
    91621 ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga
    91681 cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac
    91741 gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac gtgcaggctt
    91801 tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt taggtccagc
    91861 ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa
    91921 tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag gaatacccag
    91981 tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt cacgtcctgc
    92041 ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca
    92101 acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt ggcagccatc
    92161 atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat
    92221 ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg
    92281 caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc cctcgatatg
    92341 ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc
    92401 gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt
    92461 tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa agtgccccac
    92521 ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt caagcatggc
    92581 gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt gaggatcgtc
    92641 aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aggatttccc
    92701 gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag
    92761 ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc
    92821 atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc
    92881 acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg
    92941 ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac
    93001 tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa
    93061 ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt
    93121 atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa
    93181 aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc
    93241 tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg
    93301 gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat
    93361 taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg
    93421 aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg
    93481 aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa
    93541 tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac
    93601 gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg
    93661 gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc
    93721 tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag
    93781 gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat catgattacg
    93841 gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg
    93901 gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta
    93961 agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct ccgccactgg
    94021 atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg
    94081 caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt
    94141 cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa
    94201 atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata
    94261 attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg
    94321 ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct
    94381 ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct
    94441 acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct
    94501 tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac gggcaatcag
    94561 ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa
    94621 gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat ccttgagctt
    94681 taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt
    94741 tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa tcgagagttt
    94801 agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc gtatcttaag
    94861 ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag aaagtttaaa
    94921 agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg aggaaagttt
    94981 gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca tctccgaagg
    95041 tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc gtcgaacatt
    95101 cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga gattacaggg
    95161 tttaagcttt tgggctatca ccagaacttt aaacgatgaa ggtcacttgt cattgaatca
    95221 atacacaccc aaacaaaagg ggtggtctga tactgctgta aagaaactgc tcagaaatcg
    95281 tgctgtgatt ggatgtttca cacctgctgg acgtgaagaa gttcagggtt actaccccgc
    95341 aataatcagt gagtcactat tctatcgagt acagcaactg aacac
//
LOCUS       DAJTRM010000003        72967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRM010000003 DAJTRM010000000
VERSION     DAJTRM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.003"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO322_000238"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO322_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5905252.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO322_000239"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO322_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5905253.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO322_000240"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO322_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5905254.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO322_000241"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO322_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5905255.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO322_000242"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO322_000242"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5905256.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO322_000243"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO322_000243"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5905257.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO322_000244"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO322_000244"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5905258.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO322_000245"
     CDS             complement(4598..5320)
                     /locus_tag="OO322_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5905259.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO322_000246"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO322_000246"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5905260.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO322_000247"
     CDS             6520..6738
                     /locus_tag="OO322_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5905261.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO322_000248"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO322_000248"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5905262.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO322_000249"
     CDS             complement(7472..7672)
                     /locus_tag="OO322_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5905263.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO322_000250"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO322_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO322_000251"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO322_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5905264.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO322_000252"
     CDS             10101..12014
                     /locus_tag="OO322_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5905265.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSSEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO322_000253"
     CDS             12014..13036
                     /locus_tag="OO322_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5905266.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO322_000254"
     CDS             13030..13248
                     /locus_tag="OO322_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5905267.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO322_000255"
     CDS             13302..14171
                     /locus_tag="OO322_000255"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5905268.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO322_000256"
     CDS             complement(14226..14630)
                     /locus_tag="OO322_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5905269.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO322_000257"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO322_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5905270.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO322_000258"
     CDS             15615..17705
                     /locus_tag="OO322_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5905271.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO322_000259"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO322_000259"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5905272.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO322_000260"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO322_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5905273.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO322_000261"
     CDS             complement(19673..20275)
                     /locus_tag="OO322_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5905274.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO322_000262"
     CDS             complement(20265..20432)
                     /locus_tag="OO322_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5905275.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO322_000263"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO322_000263"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5905276.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO322_000264"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO322_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5905277.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO322_000265"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO322_000265"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5905278.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO322_000266"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO322_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5905279.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO322_000267"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO322_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5905280.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO322_000268"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO322_000268"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5905281.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO322_000269"
     CDS             28269..28436
                     /locus_tag="OO322_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5905282.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO322_000270"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO322_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO322_000271"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO322_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5905283.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO322_000272"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO322_000272"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5905284.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO322_000273"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO322_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5905285.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO322_000274"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO322_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5905286.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO322_000275"
     CDS             complement(33764..34849)
                     /locus_tag="OO322_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5905287.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO322_000276"
     CDS             complement(34861..36165)
                     /locus_tag="OO322_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5905288.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO322_000277"
     CDS             complement(36177..36530)
                     /locus_tag="OO322_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5905289.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO322_000278"
     CDS             complement(36541..37419)
                     /locus_tag="OO322_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5905290.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO322_000279"
     CDS             complement(37416..38642)
                     /locus_tag="OO322_000279"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5905291.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO322_000280"
     CDS             complement(38643..39806)
                     /locus_tag="OO322_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5905292.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO322_000281"
     CDS             complement(39890..40252)
                     /locus_tag="OO322_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5905293.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO322_000282"
     CDS             complement(40269..41174)
                     /locus_tag="OO322_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905294.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO322_000283"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO322_000283"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5905295.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO322_000284"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO322_000284"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5905296.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO322_000285"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO322_000285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5905297.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO322_000286"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO322_000286"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5905298.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO322_000287"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO322_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5905299.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO322_000288"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO322_000288"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5905300.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO322_000289"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO322_000289"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5905301.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO322_000290"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO322_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5905302.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO322_000291"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO322_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO322_000292"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO322_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5905303.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO322_000293"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO322_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5905304.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO322_000294"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO322_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5905305.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO322_000295"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO322_000295"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5905306.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO322_000296"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO322_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5905307.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO322_000297"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO322_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5905308.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO322_000298"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO322_000298"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5905309.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO322_000299"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO322_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5905310.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO322_000300"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO322_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5905311.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO322_000301"
     CDS             complement(59604..61355)
                     /locus_tag="OO322_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5905312.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQGRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO322_000302"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO322_000302"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5905313.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO322_000303"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO322_000303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5905314.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO322_000304"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO322_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5905315.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO322_000305"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO322_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5905316.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO322_000306"
     CDS             66301..68622
                     /locus_tag="OO322_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5905317.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO322_000307"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO322_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5905318.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO322_000308"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO322_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5905319.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO322_000309"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO322_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5905320.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..72951
                     /gene="rpnA"
                     /locus_tag="OO322_000310"
     CDS             72064..72951
                     /gene="rpnA"
                     /locus_tag="OO322_000310"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5905321.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17587 a        19775 c        18454 g        17151 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcagt gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcacag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgaccctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccacgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcgagtagac tggccccctg aatctccaga
    72961 caaccag
//
LOCUS       DAJTRM010000004        71139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRM010000004 DAJTRM010000000
VERSION     DAJTRM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.004"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(845..2431)
                     /locus_tag="OO322_000311"
     CDS             complement(845..2431)
                     /locus_tag="OO322_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5905322.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2786..4957)
                     /gene="glcB"
                     /locus_tag="OO322_000312"
     CDS             complement(2786..4957)
                     /gene="glcB"
                     /locus_tag="OO322_000312"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5905323.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4979..5383)
                     /locus_tag="OO322_000313"
     CDS             complement(4979..5383)
                     /locus_tag="OO322_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5905324.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5388..6607)
                     /gene="glcF"
                     /locus_tag="OO322_000314"
                     /pseudo
     CDS             complement(5388..6607)
                     /gene="glcF"
                     /locus_tag="OO322_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6618..7670)
                     /gene="glcE"
                     /locus_tag="OO322_000315"
     CDS             complement(6618..7670)
                     /gene="glcE"
                     /locus_tag="OO322_000315"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5905325.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7670..9169)
                     /gene="glcD"
                     /locus_tag="OO322_000316"
                     /pseudo
     CDS             complement(7670..9169)
                     /gene="glcD"
                     /locus_tag="OO322_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9420..10190
                     /gene="glcC"
                     /locus_tag="OO322_000317"
     CDS             9420..10190
                     /gene="glcC"
                     /locus_tag="OO322_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5905326.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10314..11455)
                     /locus_tag="OO322_000318"
                     /pseudo
     CDS             complement(10314..11455)
                     /locus_tag="OO322_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11813..13543
                     /locus_tag="OO322_000319"
     CDS             11813..13543
                     /locus_tag="OO322_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5905327.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13540..14454
                     /locus_tag="OO322_000320"
     CDS             13540..14454
                     /locus_tag="OO322_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5905328.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14487..14735
                     /locus_tag="OO322_000321"
     CDS             14487..14735
                     /locus_tag="OO322_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5905329.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14735..15906
                     /locus_tag="OO322_000322"
                     /pseudo
     CDS             14735..15906
                     /locus_tag="OO322_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15942..17021)
                     /gene="lptG"
                     /locus_tag="OO322_000323"
     CDS             complement(15942..17021)
                     /gene="lptG"
                     /locus_tag="OO322_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5905330.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17018..18088)
                     /gene="lptF"
                     /locus_tag="OO322_000324"
     CDS             complement(17018..18088)
                     /gene="lptF"
                     /locus_tag="OO322_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5905331.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18119..18679)
                     /locus_tag="OO322_000325"
     CDS             complement(18119..18679)
                     /locus_tag="OO322_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5905332.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18691..19515)
                     /locus_tag="OO322_000326"
     CDS             complement(18691..19515)
                     /locus_tag="OO322_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905333.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19515..20864)
                     /locus_tag="OO322_000327"
     CDS             complement(19515..20864)
                     /locus_tag="OO322_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5905334.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20910..21668)
                     /locus_tag="OO322_000328"
     CDS             complement(20910..21668)
                     /locus_tag="OO322_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905335.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21700..22413)
                     /locus_tag="OO322_000329"
     CDS             complement(21700..22413)
                     /locus_tag="OO322_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5905336.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22587..23279
                     /locus_tag="OO322_000330"
     CDS             22587..23279
                     /locus_tag="OO322_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5905337.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23328..24827)
                     /gene="pitB"
                     /locus_tag="OO322_000331"
     CDS             complement(23328..24827)
                     /gene="pitB"
                     /locus_tag="OO322_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5905338.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25119..26978)
                     /gene="gss"
                     /locus_tag="OO322_000332"
     CDS             complement(25119..26978)
                     /gene="gss"
                     /locus_tag="OO322_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5905339.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27183..28049
                     /gene="yghU"
                     /locus_tag="OO322_000333"
     CDS             27183..28049
                     /gene="yghU"
                     /locus_tag="OO322_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5905340.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28172..28420)
                     /gene="hybG"
                     /locus_tag="OO322_000334"
     CDS             complement(28172..28420)
                     /gene="hybG"
                     /locus_tag="OO322_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5905341.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28554..28895)
                     /gene="hypA"
                     /locus_tag="OO322_000335"
     CDS             complement(28554..28895)
                     /gene="hypA"
                     /locus_tag="OO322_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5905342.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28888..29376)
                     /gene="hybE"
                     /locus_tag="OO322_000336"
     CDS             complement(28888..29376)
                     /gene="hybE"
                     /locus_tag="OO322_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5905343.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29369..29863)
                     /locus_tag="OO322_000337"
     CDS             complement(29369..29863)
                     /locus_tag="OO322_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5905344.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29863..31566)
                     /gene="hybC"
                     /locus_tag="OO322_000338"
     CDS             complement(29863..31566)
                     /gene="hybC"
                     /locus_tag="OO322_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5905345.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31563..32741)
                     /gene="hybB"
                     /locus_tag="OO322_000339"
     CDS             complement(31563..32741)
                     /gene="hybB"
                     /locus_tag="OO322_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5905346.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32731..33717)
                     /gene="hybA"
                     /locus_tag="OO322_000340"
     CDS             complement(32731..33717)
                     /gene="hybA"
                     /locus_tag="OO322_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5905347.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33720..34838)
                     /gene="hybO"
                     /locus_tag="OO322_000341"
     CDS             complement(33720..34838)
                     /gene="hybO"
                     /locus_tag="OO322_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5905348.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35027..35314)
                     /locus_tag="OO322_000342"
     CDS             complement(35027..35314)
                     /locus_tag="OO322_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5905349.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35433..36320)
                     /locus_tag="OO322_000343"
     CDS             complement(35433..36320)
                     /locus_tag="OO322_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5905350.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36477..37517
                     /gene="gpr"
                     /locus_tag="OO322_000344"
     CDS             36477..37517
                     /gene="gpr"
                     /locus_tag="OO322_000344"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5905351.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37557..38051)
                     /locus_tag="OO322_000345"
     CDS             complement(37557..38051)
                     /locus_tag="OO322_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5905352.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38242..39126
                     /gene="yghA"
                     /locus_tag="OO322_000346"
     CDS             38242..39126
                     /gene="yghA"
                     /locus_tag="OO322_000346"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5905353.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39398..39823)
                     /gene="exbD"
                     /locus_tag="OO322_000347"
     CDS             complement(39398..39823)
                     /gene="exbD"
                     /locus_tag="OO322_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5905354.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39830..40564)
                     /gene="exbB"
                     /locus_tag="OO322_000348"
     CDS             complement(39830..40564)
                     /gene="exbB"
                     /locus_tag="OO322_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5905355.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40816..42003
                     /gene="metC"
                     /locus_tag="OO322_000349"
     CDS             40816..42003
                     /gene="metC"
                     /locus_tag="OO322_000349"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5905356.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42143..42802
                     /locus_tag="OO322_000350"
     CDS             42143..42802
                     /locus_tag="OO322_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5905357.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42842..43741)
                     /gene="yqhC"
                     /locus_tag="OO322_000351"
     CDS             complement(42842..43741)
                     /gene="yqhC"
                     /locus_tag="OO322_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5905358.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43935..45098
                     /gene="yqhD"
                     /locus_tag="OO322_000352"
     CDS             43935..45098
                     /gene="yqhD"
                     /locus_tag="OO322_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5905359.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45203..46030
                     /gene="dkgA"
                     /locus_tag="OO322_000353"
     CDS             45203..46030
                     /gene="dkgA"
                     /locus_tag="OO322_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5905360.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46230..47156
                     /locus_tag="OO322_000354"
     CDS             46230..47156
                     /locus_tag="OO322_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5905361.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47207..47464
                     /gene="yqhH"
                     /locus_tag="OO322_000355"
     CDS             47207..47464
                     /gene="yqhH"
                     /locus_tag="OO322_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5905362.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47507..49726)
                     /locus_tag="OO322_000356"
     CDS             complement(47507..49726)
                     /locus_tag="OO322_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5905363.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49837..51249)
                     /gene="ftsP"
                     /locus_tag="OO322_000357"
     CDS             complement(49837..51249)
                     /gene="ftsP"
                     /locus_tag="OO322_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5905364.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51324..52061)
                     /gene="plsC"
                     /locus_tag="OO322_000358"
                     /gene_synonym="parF"
     CDS             complement(51324..52061)
                     /gene="plsC"
                     /locus_tag="OO322_000358"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5905365.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52295..54553)
                     /gene="parC"
                     /locus_tag="OO322_000359"
     CDS             complement(52295..54553)
                     /gene="parC"
                     /locus_tag="OO322_000359"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5905366.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55099..55581)
                     /locus_tag="OO322_000360"
     CDS             complement(55099..55581)
                     /locus_tag="OO322_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5905367.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55634..56026)
                     /gene="ygiW"
                     /locus_tag="OO322_000361"
     CDS             complement(55634..56026)
                     /gene="ygiW"
                     /locus_tag="OO322_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5905368.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56178..56837
                     /gene="qseB"
                     /locus_tag="OO322_000362"
     CDS             56178..56837
                     /gene="qseB"
                     /locus_tag="OO322_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5905369.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56834..58108
                     /gene="qseC"
                     /locus_tag="OO322_000363"
     CDS             56834..58108
                     /gene="qseC"
                     /locus_tag="OO322_000363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5905370.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58154..58486)
                     /locus_tag="OO322_000364"
     CDS             complement(58154..58486)
                     /locus_tag="OO322_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5905371.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58805..59386
                     /gene="mdaB"
                     /locus_tag="OO322_000365"
     CDS             58805..59386
                     /gene="mdaB"
                     /locus_tag="OO322_000365"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5905372.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59417..59731
                     /locus_tag="OO322_000366"
     CDS             59417..59731
                     /locus_tag="OO322_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5905373.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59779..61671)
                     /gene="parE"
                     /locus_tag="OO322_000367"
     CDS             complement(59779..61671)
                     /gene="parE"
                     /locus_tag="OO322_000367"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5905374.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61700..62281)
                     /gene="yqiA"
                     /locus_tag="OO322_000368"
     CDS             complement(61700..62281)
                     /gene="yqiA"
                     /locus_tag="OO322_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5905375.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62281..63108)
                     /gene="cpdA"
                     /locus_tag="OO322_000369"
     CDS             complement(62281..63108)
                     /gene="cpdA"
                     /locus_tag="OO322_000369"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5905376.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63133..63555)
                     /locus_tag="OO322_000370"
     CDS             complement(63133..63555)
                     /locus_tag="OO322_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5905377.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63556..64185)
                     /gene="nudF"
                     /locus_tag="OO322_000371"
     CDS             complement(63556..64185)
                     /gene="nudF"
                     /locus_tag="OO322_000371"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5905378.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64390..65871
                     /gene="tolC"
                     /locus_tag="OO322_000372"
     CDS             64390..65871
                     /gene="tolC"
                     /locus_tag="OO322_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5905379.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66019..66690
                     /locus_tag="OO322_000373"
     CDS             66019..66690
                     /locus_tag="OO322_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5905380.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66696..67856
                     /locus_tag="OO322_000374"
     CDS             66696..67856
                     /locus_tag="OO322_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5905381.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67894..68682)
                     /gene="ygiD"
                     /locus_tag="OO322_000375"
     CDS             complement(67894..68682)
                     /gene="ygiD"
                     /locus_tag="OO322_000375"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5905382.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68825..69598
                     /gene="zupT"
                     /locus_tag="OO322_000376"
     CDS             68825..69598
                     /gene="zupT"
                     /locus_tag="OO322_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5905383.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69656..69826)
                     /locus_tag="OO322_000377"
     CDS             complement(69656..69826)
                     /locus_tag="OO322_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905384.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70087..70740)
                     /gene="ribB"
                     /locus_tag="OO322_000378"
     CDS             complement(70087..70740)
                     /gene="ribB"
                     /locus_tag="OO322_000378"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5905385.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70844..70992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT        17722 a        18972 c        17585 g        16860 t
ORIGIN      
        1 gcaggaaata ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataagttaa
       61 tgattttaat gattttaatg attttaatga ttttttcatt tattcacacc taataaaagt
      121 attcaaattc agatgatttt tctgctcaac agacacaaac aggcaataat actctccaaa
      181 gcgaacaata tttggcatta cgatttaatg cttcataatc accaaattat ttaaccataa
      241 attttcttat ttctaacaaa atgattggtg tgttgttgga gttaaaatac atacataata
      301 ttaacatctg ccaacatttc actttccgca agacggatga tttcgaatcc tcatccacgt
      361 taccctatcc caaatatctt tattaatagg cagtaacgaa cactgttata gcaagattaa
      421 cttattaacc taacggcatc ttttatgtaa caagtggaga taaattgctt aaaattgatc
      481 ctcgacaaat cttaaaagtt ctcattattc tgcaaaatca gctttgcgaa taatcttatg
      541 tttttgggag ttttgggaat tttgatctac tcattgttct agtgaaaaat ataaataaga
      601 gaaaaaacgc cattcgtcat gaaagcaaca gatgaagcaa tgtaataaca tactaaaatt
      661 gatctcatga aagtgggtac tttataacca gcacagaagc caagttgtat aaatattaaa
      721 caattcgcat cgtaaaacgg agactacgta taaagaagcc aatcgtaaaa acggttccct
      781 ggcgagataa acgacagaca aatggaaagc cgggtgatat ttaccttcat ccggctttat
      841 tgatttacga gactaacatc ccggtaaaca catacgcctg cagcagggtg ataacgctgg
      901 caaaaatcag actgtgcttc acggtgtagc ggaacagttc agattctcgg cccaccatgc
      961 ccgtcgcggc gcaggccacg gcgatagatt gcggggagat catcttgcca gttacgccgc
     1021 cgctggtatt tgctgccacc agcagagtgt cagagacgtt gatttgctgc gccgtggtcg
     1081 attgcagtga accaaacagg gcgtttgaag aagtgtccga cccggtaagg aatacgccca
     1141 gccagccgag aaacggtgag aaaaacggga acatcacgcc tgtacctgcc agtaccagcg
     1201 ccagcgtggt ggacatgccg gaatagttgg tgacgaaggc gaatgccagc accatgccaa
     1261 tcgacaatat cggccacttc agactaatta gcatttcggc aaagacgcca atccctttct
     1321 tgatgcccac gccgaggatg aagatagaga taatcgccgc aataaaaata gcggtgccgc
     1381 cagccgagag agggtcgaat ttaaacaccg catccattgg cgttggttgg gcgacaatgg
     1441 gtgccgcttt caacacttgt tgatgcaaat gagggatctg gaaattaatc accagtgaat
     1501 aaaacgcgcc gcccggagca aataacgctt taaacggctt catggtccag atggtgacca
     1561 gcaccgttaa gattaaaaac ggtgaccacg cacgaatgat ttgccccaga ctatattctg
     1621 aaggcacggg accgccagaa gatggcttat ttaccaccat cgcacctgcg gattgcccca
     1681 tgctgattgc cgtttcggta tttttcggcc gccagacttt aaggaataaa gcgagtgaga
     1741 cgatactcac cagcgccgaa gtaatatccg gcagttccgg accaatatag ttagaggtaa
     1801 agaactgagt gacagcgaag cttcccccag caaccagcgc cgctggccac gtctctttca
     1861 cccctttcca gccgtccatc attgctacca gccagaacgg cacaagaacc gacaggaacg
     1921 gtaactgacg tcccgccatt gcgccaatgt ggaacggatc gattcccgtt acctgaccgg
     1981 cgaccagaat cggcacgccc aacgcaccaa acgccaccgt cgcaccaaag ccagcagctc
     2041 cttccagcaa cgcaccaaag gagaaaccaa tcagtaacac ctgcaaacgc tgatcgtcgg
     2101 tgatggagat aaccgagctg cggataatat cgaactgccc gctggcaacg gttaatttat
     2161 acaggaacac cgccgcgaca ataatccacg caattggcca taatccataa ataaagccat
     2221 agcccgcagc agcaaatgcc atatcaatcg gcattttaaa ggcgaatatc gcaatcagga
     2281 tagataatat aagggttatt gctccagcaa catgtccttt cagacgtaat accgcgagtg
     2341 caacgaagaa gaatattatc gggatcaggg cgaccagagc ggatagcccc agtcctccca
     2401 tcggcatata catttgggtc caggtaacca tattgttcag tctcttatta tctttatatg
     2461 cttgatatac ttaaggttgt aataagcaaa agaggactga actgtaaaat ataggcgtta
     2521 tactttacag caacagtacg ccgctaacgc aattgctacc tctggcataa caagtatatc
     2581 gggtaagggt ttctgttccg cacacgcagg cgcagagtat cgttaagatg tccatattgt
     2641 tgttttaggc ccgctagtaa tgcgctacgg gtatttaata ttgttaaacc ctgataatcg
     2701 ctccggttat ttccgggata aatgtactac cgcagttact atcatagccc cgacaataaa
     2761 acttgccggg gcttttttga cgctattaat gactttcttt ttcgcgtaaa cgccaggcgt
     2821 gtaataacgg ttcggtataa ccgtttggct gtttcacgcc gaggaagatt aaatcgctgg
     2881 cagctttaaa agcacacgag ttagcgaaat tccccgccat cggacgataa gccggatcgc
     2941 cagcattttg ctgatcaacc actttcgcca tattctccag cgacgcctgc acctgttctt
     3001 tggtcagaat accgtgacgt aaccagttgg cgatatgctg gctggagata cgcagcgttg
     3061 cgcggtcttc catcagcgcc acgttgtgaa tatccggcac ttttgaacaa ccaatcccct
     3121 gctccaccca acgcaccacg taccccagaa tcccctgcac gttgttatcc agctcttgtt
     3181 ggatctcttg cgccgaccag ttagcgtttt cagccaccgg aatagtcagc agatcgtcca
     3241 gcagcggttc aaattcagca ctgaactcgg tctgggcaat gttggcttgc acgctctgta
     3301 cgttggtatg gtggtagtgc agcgcatgga gcgtagcagc ggttggtgac ggaacccagg
     3361 ctgtgtttgc cccggcacgc agttggtcgc ccttctggct gtacatgtct gccatcaggt
     3421 ccggcattgc ccacatgcct ttaccgattt gcgctttacc gcgcagccca cagaacagac
     3481 cggaaagcac gttattacgt tcgtaggctt tgatccacgg cgtcgatttc atctggtttt
     3541 tacgcagcat cgggccagct tccatcaccg aatgcatttc atcgccggta cggtcgagga
     3601 aaccggtatt gatgaacgcc acgcggttgc gcgcctgagc gatacagcta cgcaagttca
     3661 gcgaggtccg gcgttcttca tccataatgc ccattttcag ggtattcggt gccataccga
     3721 gcattgtctc aatgcgggta aacagtttgt tggcgaacgc cacttcctgc ggaccgtgca
     3781 ttttcggttt cacaatatag acgctgccag tgcgcgagtt tttctgcact tttaaatcat
     3841 agagggcaat cgcgccagtc atgacgccat caagaatacc ttccgggatt tcattgcctt
     3901 cgctgtccca aatcacagga atggtcatca aatgacccac gttgcggata aacagcaacg
     3961 agcgtccgtg cagagaaatt tcagagccat cggcggcggt gtactgacga tcgtcattca
     4021 gtttacgtac gatttgccga ccgtttttct ccattttctc ttgcagagtc ccctgcatca
     4081 ggcccagcag attgcggtac agcaggattt tatcttccgc atcaaccgcc gcgaccgaat
     4141 cttcgcagtc gagaatggta ctgatagcag cttcgacgat aacatcgttg atgtgcgccg
     4201 gatcgtcttt gccaatccgc ccgttggcat caatttgcag ttcaatatgc aggccgttat
     4261 ttttcagcaa aatgcaggtc ggcgcagcgg catcgccacg gtaaccgaca aactgtgctg
     4321 gagtacgtaa cgtggtttct ttaccatttt tcaactggat gcgtaattgt ttatcgacca
     4381 ccttaaacgc caccacatcc tgatagctgc cgttttccag cggtagtgat tcatcgagga
     4441 aacgtcgaac ccaggcgata acctgctcac cgcgctgcgg atcgtagcca ctgaccatcg
     4501 ccccttcctg cgggatgatg tcgctgccgt ataacgcatc gtacagtgag ccccagcgag
     4561 cgttcgccgc gttcagcgcg tagcgggcgt tcattgccgg aaccaccagc tgtggccccg
     4621 cctgactggt gatttcgctg tcaatgcccg tggtttccac cgtcacgcgc tccggttgcg
     4681 gcaccaggta gcccagttca cgaaggaaag atttataggc cgctttatct tttaccggcc
     4741 ccggattgct gcgatgccac tcatcaagcg ccgcctgaat gcgatcgcgt tctgccagca
     4801 actgacgatt ttctggtgcc agatcgtgaa cgatctcatc aacattgtgc cagaatgcag
     4861 cagcgtccag ttccacgccc ggcaaaactt ctccatccac aaaacgttta aaattggcgt
     4921 caatgcgtaa acggccctgg gttatggttt gactcattgt ttctctcctc gttttcgctt
     4981 atttcgccaa caccgctgct gccactttcg cgacctgcgc atcctgtgct ccggttaaac
     5041 cagaaacgcc cacggcacca ataatttgcc catcaacaac aaccggtacg ccgccttcca
     5101 gcgacgtcag taacggcgca gtcacgaacg cggtacgtcc gttgttcacc atctcttcat
     5161 agcccttagt ttcacgacgc cccagcgcgg cggtacgcgc tttctcctgg gagatgtaag
     5221 ccgcaatcgg cgcgcaatcg tccatgcgac ttaacgccag cagatgaccg ccgtcatcgg
     5281 caacagcaat ggaaacagac cagttatttt tctgcgcctc ttcctgacct gcggcaataa
     5341 ttgcactcgc catttgctgg ctaagaatga ctttagtttt cattttttta ttccttttca
     5401 agggcttgtt ctacaatttc aatccagtga cgcacagagg tacgaccggc gctcgccaga
     5461 tgcgtctggc aaccaatgtt ggcggtgacg atcatttccg gtttgccgct ttccagcgca
     5521 ttcattttgt tatcccgcag ctggcgtgcc agatcgggat gcgttaacgc atatgttccc
     5581 gctgaaccgc agcacagatg gctgtcggga acgtccgtta aggtaaatcc aagacgaagt
     5641 aacacttttt ccacttcgcc gttcagcttt tgcgcatgtt gtagggtaca cgggcagtgg
     5701 aaggccagtt ttttatcgcc gcgaattgcc agtttttcca gcggttcctc gcgcagaagt
     5761 tcgactaaat cgaccgccag ttcactgacc tgacgcgctt tatcggcata taacgcatcg
     5821 tttttcagca tctgcccata ctctttgacg aacgcgccgc aaccgctggc ggtttgcaaa
     5881 attgcctcgg cacctgcttc aatcgcgggc caccaggcat caatattatt gcgcgcccgt
     5941 gccaaccctt tctcctgcgc attaagatga tagtccaccg cgccacaaca gcctgcttcg
     6001 ttagctgaca tgacgctgat ccccagacga tccagcactc gcgcagttgc cgcgttggtg
     6061 ttgggcgaaa gcgtaggctg ggcgcagcct tccagcatta aaacccgacg cttatggcgc
     6121 agcggcggac gcggtttagc tttcaccgtt tcagcaggca gttttgctct gacctgttcc
     6181 ggtaaaaacg gtcgcagcac cagccctacc tgcgtcagcg cacggaagac cgccggacgc
     6241 ggcactacct ggcgcaatcc ttcgcgcagc attcgctccg gcagtgggcg tttcactttc
     6301 tgctcgacaa tatcacgccc gatatccagc aaattgtgat agcgcacacc agaaggacag
     6361 gtggtttcac aattgcggca agtgaggcag cgatcgagat gctcctgtgt tttaagcgtg
     6421 acttcgttgc cttccagcac ctgtttaatc agatagatgc gcccgcgcgg cccgtccagt
     6481 tcatcgccca gaagctgata ggttgggcag gttgcggtac aaaatccgca gtgaacacag
     6541 gcgcacagga tgctgtcggc ttccagcgcg cgttctgccg catctcttcg gttaatcggg
     6601 tttgcatagc ctgctcctca aagttccgcg tacatgcgac cggggttaaa cacgccgcaa
     6661 gggtcgagct gctgtttaag ctgctggtga tagcggaata aaggagccga tagcggggca
     6721 aagccaccat ctccggcact aaagcgggtc gcatgaccgc cagcgttgcg ggcgatgcga
     6781 tggatttgat tgtcatcggc tgtcgatttc agccagcgta acgccccgcc ccagtcgatc
     6841 agttgctcgc cgggtaaatc catcatcggc gcatcgctgg gtaatgaaat gcgccataag
     6901 gtacctggta acgagaagaa cggcagttgt tgttcacgca attgctgcca gaactgaccc
     6961 acaacctctt cgccacccag cagttcacgc gctgctttta ccgatccttc gccgccctca
     7021 aggcggatcc acaacgcatt gtcgaagtaa cataagccac taatgggtaa tggctggagt
     7081 tgccactcgg cgatttcact catggcttct tgcaggctga tttcccgacg caggctcaga
     7141 gaggcacgcg gtcgcggtaa cactttcatt gagatttcag tgagcacgcc aagacaaccg
     7201 tagcttccgg ccattaaccg tgagagatcg tatccggcaa cgtttttcat cacttcgcca
     7261 ccaaaacgca gatgttttcc agtgccggta atgatgcgcg tgccgaggac aaaatcgcgg
     7321 accgaaccgc tccacgggcg acgcggcccc gccagcccgc aggcgaccat cccgccccag
     7381 gtggcttctt caccataatg cggcggctcg cagggaagca tttgcccggc gctctccagc
     7441 gccgcttcaa tcgccaccag cggcgttccg gcacgagcgg ttatcaccag ctcggtcggg
     7501 tcgtaattaa caatgccgcg atgacaacgc acatccagcg tttgcccggt gactgggcga
     7561 cctaaaaagg ctttgctatt gctgccctga atcaccagcg gcgttttatc gctaattgcc
     7621 tgattcacct gctccagcag cgcctgactg taatcacact cgcgtagcat caaaagcgct
     7681 ccagttcagg aaaaggtaaa tgaccgtgat gcacatgcat ggcaccaaat tcagcacagc
     7741 ggtgtagcgt gggaatgttt ttcccagggt tcagcaaacc atcggggtca aacgccgcct
     7801 tgaccgcatg gaaggtcgtg atttcatcgc tgttgaactg ggcgcacatt tgattgattt
     7861 tttctcgccc gatgccatgt tccccactga tgctgccgcc aacttcaacg cagagttcga
     7921 ggatcttccc gcccagctct tccgcgcggg caaattcacc aggttcgttg gcatcgaaaa
     7981 ggattaacgg gtgcatgttg ccatctccgg catgaaagac gttggcaaca cgtaaatcat
     8041 attgctgcga taaacgggca atgccttcca gtacgccagg cagggcgcga cgcgggatgg
     8101 tgccatccat gcagtagtaa tccggggaga tacgtcctac cgccgggaac gcatttttgc
     8161 gaccggccca gaaacgtacg cgctctgctt cgtcctgtgc caaacggacg tcagtcgcgc
     8221 ccgctttcaa caagatgtag ttaacccgct cgcagtcttc ctgtacgtca gactccacgc
     8281 cgtccagttc gcataacaaa atcgcttcgg cgtcgacggg ataaccggca tgaataaaat
     8341 cttccgccgc gcggatcgac aggttatcca tcatctccag cccgccgggg ataatgccat
     8401 tggcgatgat gtcaccaacc gcaagtccgg ctttttctac cgagtcaaag ctggctaaca
     8461 gaacccgcgc cacgggcggc ttcggcagca gttttaccgt cacttcggtg gtcacgccga
     8521 gcataccttc cgatccggtg aacagcgcca gcaggtcaaa accaggtgaa tccagcgcgt
     8581 ccgatcccag cgtcagcgcc tcgccgtcca gcgtttgcac ttcaattttc agcaggttat
     8641 gtacggtcag accatatttc aggcagtgga cgccaccggc attttccgcc acattgccgc
     8701 caattgaaca ggcgatttgt gaggaaggat ccggcgcgta atagagatta tgcggcgcaa
     8761 cggcctagga gatcgccagg ttacatacgc ccggctgcac gcgcgcgcgg cgaccaacgg
     8821 ggttaatgtc gaggctctct ttaaagcgcg ccatcaccaa caacacacct ttttccagcg
     8881 gcagcgcgcc accagaaagc ccggtgcctg caccacgggt caccaccggt acacgcaggc
     8941 gatggcagac agccagaatc gctgtcacct gttccatttg cttaggcaga acaaccagta
     9001 atggacgcgt gcgatacgcg ctcaacccgt cacactcgta aggaatgatc tcctcatcgg
     9061 tatgcaggat ctcaagtcca gggacatgct cacgcagtgc catcagtacc gatgtgcggt
     9121 cgacatcggg taaagcgcca tcaagacgct cttcgtacaa gatgctcatg agtaggcttc
     9181 gctttgttgt gttgtgtggc agctgatttt tgcgcgctgc ttctgtgaac agttattaag
     9241 cgggcttttc gttttcgtct atctctttag ctaccggtca gaccattttt tttccagctc
     9301 tgtgaccttg tcttggttaa cttaatgtta aattgatgta acataatcac ttgcgtgatg
     9361 tgcgtgtttt gcgagttaag aacagaaaaa ttggtcctac ctgtgcacga ggtccgggaa
     9421 tgaaagatga acgtcgccct atttgcgaag tggttgcaga gagtatcgaa cggttaatta
     9481 tcgacggcgt actgaaggtc ggtcagccgc ttccctcgga acgtcgactg tgtgaaaagc
     9541 tcggcttctc acgctccgca ctgcgtgaag ggctgaccgt gctgcgcggg cgcgggatta
     9601 ttgaaacggc gcagggtcgc gattctcatg tcgcacggct taatcgggag caggacacca
     9661 gcccgctgat ccatctgttc agtacgcagc cgcgaacgct gtacgatctg ctcgacgttc
     9721 gcgcattact ggagggcgaa tcggcaaggc tggcggcaac gctgggaacg caggctgatt
     9781 ttgttgtgat aacccgctgt tatgaaaaaa tgctcgccgc cagtgagaac cacaaagaga
     9841 tttcgctgat cgaacatgcg cagttggatc acgctttcca tctcgccatt tgtcaggctt
     9901 ctcacaatca ggtgctggtg tttacgctgc aatcattgac cgatctgatg tttaattcag
     9961 tgtttgccag cgtaaataat ctctaccatc gaccacagca aaaaaagcag atcgatcgcc
    10021 agcatgcgcg gatctacaac gcggtgttgc agcggctgcc gcacgtcgct cagcgcgcag
    10081 cgcgcgatca tgtacggacc gtgaaaaaga atctccacga tatcgagctg gaaggccacc
    10141 atttgattcg ctcggcggtg ccgctggaga tgaacaaggt gggaatgtga gcagagtttt
    10201 accctatccc caaggcgagg ggaccgcccc tgcacttaac aggatctggt gttaaatctg
    10261 ttcatctata ccctctcccg aatgaccgcc cctgcgctaa acaggatctg gtattaaatt
    10321 tgcttttcat acaatcggta acgcttgtac ggctccgccc caatgcgttc cagcatgtta
    10381 ttcatgcctg tattggtttc gaggatccat gacatctcca gcgcatcgat cttccggcgg
    10441 gcaaacggat cgcgtaaggc ttcaatcaat aacagcgcaa tcaccgggcc gatgcggctg
    10501 aactgatact cgtcgcgcac gcccatcagc ggtactcgcg cagttcgcac accgctgact
    10561 ttcaaacgcc acagcaattt tgcccagccg aagggaaaga gcgatccgtt cagatcggca
    10621 atcgcctcgt tgatgttagg caagccacaa taaacgcgca gggtgcagaa tcaatctcag
    10681 cgatgtagat catatcgtcc ggcaccagat atttaagttg gtcgcccatg gtcgcgaatt
    10741 catgttcggt aaacggcaca aatccccagt tgtgctgcca gccagagttg aaaatctcac
    10801 gcaggatctg catctcttcg gcaaaccgct gacgattgat gcagcgaatg gtcacctttt
    10861 tgcgcacctg atccatcagt ttttttagcg ccggagagaa agtgagatcg gttcgctgca
    10921 tccaccacgc cagtaaatca atgcctttgt gataacccag ttgttcaata tgcgcggcat
    10981 accacggttt gccgtgtggc atcatcgcac agggtggtgt gtcaaaacct tcaatcagta
    11041 atccgctttc ctgattgata ttcaggctga aaggcccgct gatcttactt gcaccttgtg
    11101 acttcaacca cgcttccgct gcgccaaaca acgcggcaaa atcctgcgga tcatcaatgg
    11161 cgtcaatcat gccgaaatga ccggtatctt caccgtaacg ctcacggtgc aaggtatcta
    11221 tttgcgcggt aatacgccca actatctgcc ccgctttttt tgccacccac gcctgccaga
    11281 tgatatggtc cgtccccgga tttttcgcag acaaatgctc gttgcgttca atgaataaag
    11341 ggggtatcca gtttggatcg tcgggataaa gtgatgacgg aaaagcgata aatgccttaa
    11401 ggtcattttt attaattact ttttcaattt taatcatcag cagatcgcat tccatatctc
    11461 tgtggataac aaataattaa tggtgaaaaa caggttgcaa cagcaacgag cggcatctta
    11521 ataatcaact caatgaatac aaacgacgaa taaatagtat taattaatga ttatgataag
    11581 gtcagaggct acacagaaac gcctcaatgc cttttgtcaa tttaaggcga ccggacaagg
    11641 cttcttctta atatattcat aaattaaata tcattcataa taaaaaaaac ttaaactcat
    11701 tatttaaatc aatgttcatt ttcacaccga tgaaaatact atctcattta tcttttatgc
    11761 ctatataatc caccgcatta ttgtcatttt atttttgata tccaattttt tcgtggtgta
    11821 tatgtctaat aaaatcttta cgcattccct acctatgcgc tatgccgatt ttccaacgct
    11881 ggttgatgct ttggactacg ccgctctgag tagagccgga atgaattttt atgacagacg
    11941 ttgccaactt gaagatcaac tggaatatca gacattaaaa acgcgtgccg aagctggtgc
    12001 gaagcggttg ttatcgctga acctgaaaaa aggcgaacgc gtggcgctga ttgccgaaac
    12061 gagtagcggg tttgtagagg ctttttttgc ctgtcagtat gctggcttag tcgccgtccc
    12121 gttggcgatt ccaatgggcg tcggtcagcg ggattcctgg agcgccaaac tccagggttt
    12181 actggcaagt tgccagcccg cagccattat cactggtgat gagtggttgc cactggtcaa
    12241 tgccgcgacg cataacaacc ccgaattaca tgttttaagc cacgcttggt ttaaggcatt
    12301 accggaagcc gatgttgcgc tccagcgtcc agttccgaac gatatcgcct acctccagta
    12361 cacctccggc agcacccgtt ttccccgtgg cgtcattatc acccatcgcg aagtgatggc
    12421 taatctacgt gctataagcc acgatggcat taaattacgc cctagcgacc gctgcgtctc
    12481 ctggcagcct ttctaccatg atatgggact ggtcggcttt ctcctgcccc ccgtcgccac
    12541 gcaactttca gtagattatt tgcgcactca ggattttgcc atgcgtcctt tgcaatggct
    12601 taaattgatc agtaaaaatc gcggcaccgt ttccgttgcg ccgccgtttg gctatgaatt
    12661 gtgccaacgc cgcgtgaatg aaaaagatct cgctgaactg gatctttcct gctggcgcgt
    12721 cgctggtatt ggtgctgagc cgatctccgc agaacaactc catcaattcg ctgaatgttt
    12781 ccgtcaggtt aactttgaca ataaaacttt catgccgtgc tacggactgg cagaaaatgc
    12841 gctggctgtc agcttctctg atgaagcctc cggggttgtg gttaacgaag tggatcgcga
    12901 catcctcgaa tatcagggta aagccgtcgc gccgggtgca gagacacgcg ccgtatcgac
    12961 tttcgtcaac tgcggcaaag cgttgccgga acatggtatt gaaatccgca atgaagcagg
    13021 tatgccggtc gcggaacgtg tggtaggcca tatttgcatc tccggtccca gtctgatgag
    13081 cggttacttt ggcgaccagg tttcgcaaga cgagattgcc gcgacgggct ggttagacac
    13141 cggcgacctc ggttatctgc tggacggtta tctgtatgtc accggacgca ttaaagatct
    13201 gattattatt cgtggccgta atatctggcc gcaggatatt gaatatatag cggaacagga
    13261 accggaaatt cattctggcg atgcgattgc ttttgttacc gcccaggaaa aaatcatttt
    13321 gcagatccag tgtcggatca gcgacgaaga acgtcgcggg cagcttatcc acgcgctggc
    13381 ggcacggatc caaagcgaat ttggcgtgac cgcggctatc gatctgttgc cgccccacag
    13441 tattccccga acgtcctccg gcaagcctgc ccgtgcggaa gcgaaaaaac gttatcagaa
    13501 ggcttatgct gccagtctta atgtgcagga atccctggca taaatcaaac tgtcgcggtg
    13561 acgggcgcta ccgggtttat cggtaaatat attatcgata acctgctcgc ccgcggcttt
    13621 cacgttcgcg cattgacgcg tactgcccgc gctcacgtca atgataatct cacctgggtg
    13681 cgcggttcgc tggaagatac tcattcactt agcgagctgg ttgccggagc cagcgtagtg
    13741 gtccattgcg ccgggcaagt gcgcgggcac aaagaagaga ttttcacccg ctgtaacgtt
    13801 gacggcagcc tgcgcctgat gcaagcagca aaagagagcg gcttttgcca acgttttctg
    13861 tttatctctt cgctggcggc gcgccatccc gagctctcct ggtatgcaaa atccaaatac
    13921 gtcgccgagc aacgactggc agcaatggcg gacgaaatta cgctgggcgt ttttcgcccg
    13981 acagccgtgt atggtcccgg cgataaagag ttaaaaccgc tgtttgactg gatgctacgc
    14041 ggcctgctgc cacgacttgg tgcgccagat acacaactct cttttcttca cgtcaccgat
    14101 tttgcgcaag cagtgggtca gtggttaagc gccgaaactg tacagacgca aacctatgaa
    14161 ttatgcgatg gcgtcgccgg tggctatgac tggcaacgcg tacgacagct tgccgccaaa
    14221 gcccgttgtg gttccgtgcg aatggttgac attcctctgc cggtactcac aggccttgcg
    14281 gatatcagta ccgcgttgag tcgcctggca ggtaaagaac ctatgctgac ccgctcgaaa
    14341 attcgtgaat taacccacgc cgactggtcg gcaagtaata accgtatttc tgaagatatc
    14401 aattggtttc ccgggattag cctggaacac gcattacgca acgggctatt ttgattgtct
    14461 tgttttaaag gtgtgttaac gtttctatgg taaatcgtga aatagtaatg gattacatcc
    14521 tttcctgttt acaggatttg gtcgaaaacg gtgtggaaat taagcccgac agcgatctcg
    14581 tcaacgatct tggcctggag tcgatcaaag tgatggatct cttaatgatg ctggaagaca
    14641 gattcgatat ttccattcct attaatattc tgctggatgt caaaacccct gcgcaattaa
    14701 tggaaactct acttccatgg ctggagaata aataatgggg ctatacgata aatatgcgcg
    14761 ccttgctggc gagcgtctgc aatttagtga caacgggtta acaccgtttg gcacctgtat
    14821 tgatgaagtg tattccgcca ccgaaggccg gatcggcaat aaaaaagtga ttctggccgg
    14881 gaccaataat tatctcggtt taacctttaa tcatgatgcc atcagcgaag gtcaggccgc
    14941 gttagcggcc caaggcaccg gcaccaccgg ttctcgtatg gcaaacggca gttatgcgcc
    15001 gcacctggcg ttagagaaag agattgccga gtttttcaat cgccctaccg ctatcgtttt
    15061 tcaaccggtt ataccgctaa tttaggcgtc atcagcgcac ttgccgacca taacgccgtg
    15121 gtgttactgg atgccgacag ccacgccagt atttatgacg tctgttctct cggtggcgcg
    15181 gaaattattc gtttccgcca taacgatgct aaagatctgg aacggcgcat ggttcgcctc
    15241 ggtgaacggg cgaaagaggc gattatcatc gttgagggca tctacagcat gctcggtgac
    15301 gttgcgccgc tggcggaaat tgttgatatc aaacgtcgtc tcggtggtta tttgattgtc
    15361 gatgaagcgc attcgtttgg cgtgttaggc gcaacggggc gcggactggc tgaagctgtc
    15421 ggcgttgaag atgatgtgga tattatcgtt ggtactttca gtaaaagtct ggcctcgatt
    15481 ggcggttttg ctgtcgtttc ggaagcgatg gaagtgctgc gctacggcag ccgcccgtat
    15541 atttttaccg cctctccttc tccgtcctgc attgcgaccg tgcgctcgtc gttaagaact
    15601 attgccacac aaccagagct gcggcaaaag ctgatggata acgccaacca tctgtatgac
    15661 ggtctgcaaa aactgggtta tgagttaagt tcacatatta gcccggtagt gccagtaata
    15721 atcggttcga aagaggaagg tttacgcatc tggcgcgagc ttatctcgcc gggcgtgtat
    15781 gtgaatctta tcctgccgcc tgccgcgcct gcaggcatca cgctgctgcg ctgtagcgta
    15841 aatgctgccc acagtcgtga gcaaatcgat gccatcattc aggcatttgc tactctgaag
    15901 cagtaatgtg agcccgccgt ggtgtttgcg ccacggcggg tttaatgttg tctgtcatac
    15961 caatacacca ggctcaacgc caccaacaca aacgctaccg gtaaaccgag cgcggtaaca
    16021 ggtgcgctta acgcaaatag caatcccaga ttaaccatga tttgatagct gatccaggtg
    16081 agtaagccca cgatgacgcc tacagcgaga cggctaccca tcccaggcga acgcggggca
    16141 ctaaaggtaa acggcacagc cagcaaaatc atcgccaggg tcaggatggg ttgccccagt
    16201 ttttcccaca gtgcaaggtg gtattcgctg ctgggttgcc cggtttcctg caaataatgg
    16261 atgtaatgat taagctgacg gacagaaaaa gtattccccg gcattgataa ctcttcaaga
    16321 ttcatactgg tgaaggccga ttgccaggca agattatcta atgtttccag cgtttctttg
    16381 ccattcagcc attttttatg attcacacca tgcaggaccc acgttttgtc gtcttcaatg
    16441 gtggcactgc gtgcataaat gtaggattca agcgagagat cgtcgcgata atgaaatatc
    16501 tccacgccca ccggctggcc ctgctcgttc agcgatttca ccgtcacaaa ttcattacca
    16561 cgcctggccc acagcatatt gccggtaatg tcatcgtcct cccccaacgc ggtggcggtc
    16621 gatttgattt gcaacgcctg ttgctgcaat ggcgacgcca cccactcatc tatcgcgcct
    16681 aacgaaacag tccacaatat cccggcaacc agcgccacca gtgcaatacg gaagatagaa
    16741 aaccccgtgc tgcgaatggc ggtaagctca ctgtttttcg ataactgccc cagtccaaca
    16801 ataccaccca ataacgcgat aaacggcgaa agttcgacca gtgtgcgcgg taaggtcatt
    16861 agcaccacca gcaccgcctg agtccagcga taaccgcccg ggctgacacc atccagttcg
    16921 ttaatcaggt tgaaggtggt aaaaagcggc agcaatagcc ctgcagcggc ggcaaaaccg
    16981 agaaagagat gacggattaa atagcgactg aaaacattca tcgggaaaat ttctgcagca
    17041 aagagaagtc acgtgcgacc agtatcagca ggcccattag catgagtccg ggcactaacc
    17101 acaaaccagg aatgagggga atcgcgccgt tagccaccag tgtacggcag acgtcgccgc
    17161 cataaaaaat ggcaataaac agcaacgtca gtggcaataa cgttgaaaat cgcccttgcc
    17221 gcggttttac ccgacttaat gaaatggcca gcagcgccat caacaatgcg gtcaggccac
    17281 ggctttgtcg ccattgcagt tcggcatgat cggcaggaaa ggcggagcgc gccagctccg
    17341 tgaccgattt cgctttgcgt ttgacgttcg ggctttgatc cagcggattc agatgtaatt
    17401 gcagattacg gtagatctgt tcgttgtcgt cacgcccctg atgatcgaga agataggcag
    17461 tgccgttgtg caacataacg gtcggtttct cgggtgatgg gtcaaccaca tcaaccgaac
    17521 cggcgcggaa aatacgggtt cgattggcag tagaagtata aatcagcgcg tcagtcaggc
    17581 gattattatc ctgatcaacc gtctgcgaaa ggatcattcg tccgttatcg ttagtgttaa
    17641 atttctttgc ccgcaactgg cgaacatcca gctccgactg cgactgctgc tccagttgat
    17701 aaatctgcgc ataagcccac ggacgaccat acatcgacag aagggtgaca aaaatactta
    17761 gcggtatcgc cagataaaga acggctttgt acaagcgtcc cggactgccc cccgccgcag
    17821 agatagcggt aatttccgag tcggtataca tctgccctag cgtcacgcca actgacacat
    17881 acagaccaac aggtaacaac atctccagtg caatcagtac tttgtagaaa acgatatcca
    17941 gcacaacatc aagagctaat gtcccatttg ccgcttcggt cagataacgc tgtgcggagt
    18001 agctggcgaa aataaagatc agaaaaccga cgataatcag caccagacgg cgcgtaccac
    18061 gcatgatgta gtgttcaacc agcttcatct cactaacgcc ctcaatcctt tgagtttttt
    18121 attcattgat cagtttctgc gccaacgcaa tgacatgatc tacctcttcc ggcgtcaggc
    18181 tgccgtcctt tgcccactga atttgcccgg ctttattcag caccagaatg gttgaactct
    18241 gctccggcaa gcgccaggca acgcgtccct gtccattgcc gtcaataacg aattgcgccc
    18301 acggatagcg gcgtttattt ttttcgattt tgccaacaac aaaatagcca gtaccaaaga
    18361 tggcatcatc ggtgttcaca atggtggtgg gctgaaaacg atcctgcgga aagtttgccg
    18421 cttcaaccgc tttaattaac agtgagttct ttttcttcgc cgacttacgc ccggcgatat
    18481 actgaataat gcggactttt cccgctaatt gtgtgctgtt ccacttttgg tagtgaattt
    18541 cgtcgtcgct attcagaagc agctcgccgc cttcctggat ataaacaggg gtaaccgttt
    18601 tgccagtgac gaaattgtgt gcctgcacac ccggggctaa gagcatgata atcaacgcaa
    18661 taatcaaacg agatgacatc attgctccgg ctatagcgga aaacgcgaaa tcagcagctc
    18721 taagcgctga caaatatcgc tatccagcgc caacggtgca taatacgact ggcggttttc
    18781 catatcatcc gccaccatgt cgtcgtgttt atctcccatc aggccattaa tggcggcgag
    18841 gaaacgggaa atctgtaacg cctctttatt ttcaggatca agcgatctta aggtgtcgta
    18901 ataagaaacc tgctggctga tgatttttcg cacatcatga aattccctgc cggttagtcg
    18961 cggtttggca ataatctctt tcagggtatg catttgcttt tgccgatagg caataaaact
    19021 gcttacgctt tctaactggc tatggcgtaa tgaatagttc accaacgacc agaccggaga
    19081 gcttaaataa atccgtaaca ggttgccgta atacgacaca atatttttct taccgttacg
    19141 aaagccgtcc tgaaaacgcc ccagaaaaac ggtcgttttg ccgaataaaa atccggcctg
    19201 atgcttcttt aaatacagcc gttgcgcaaa acgcaggtgc ttgtagcgcg ctcgcatgta
    19261 tttaaattgt ttgcgctcat cagcagtcag ctcatcgcct ttggcttgtt tgcgaactaa
    19321 acgcagtaat tcctgacgat caacgccatt gagccagaat tgcaggcata acgcgtaatt
    19381 atcatgaatg atttcggtgg tacaacttag tgcaatagtc ggcggcaaac taacctgtgc
    19441 atcaacgaca tcatcttctt caatcgcaac aaatagacgc tcgctcgcct caagcgggaa
    19501 atattttctc atcatcagga tttctctccg gctgcgttat aaagcaagga atggcgctga
    19561 aaaaatgcct ttaaggttgg aattaacgag agcgccacgt ccagacattg cccaccgaag
    19621 taagcaaacg ctgctcccag cacaccaaac agatgacaga acatgaacag caatacgatg
    19681 tagccgattg aggcgatggt ttgcgccacg agaaaagcgc gctgttttcc cgccatcaat
    19741 agcaatgact cctgcgggaa gcccagcatc gaaatcacaa ttgcgcccag catcacctga
    19801 atcaggtcat acgcttcgag atacttaacg ccaaacacca gcgaaatgag cggcttgccg
    19861 acgatcaaca ccgcgagcgc caccagaata ccgattccgc ccgccagtaa accggacttc
    19921 acacctaaca accacggtct ggtggtgcgc ggatctaaac gcatcacctc tgggtaaaaa
    19981 cttttaccca gcaaacccgc tggcgttccg gcggcgtcga agaatgtcat ggcgatttta
    20041 aataacccgg cggcagcggg tcctaacacg atccccacca acactgtgct gcacgagtta
    20101 cgcgccgacc agatggagtg ggcaatgttg gttgaccaga caaaactcca cgcgccttta
    20161 atgtgtcggg cagactcaaa cagattcaat ttgaaggcgt tatggatatt tctacggcgt
    20221 aattcgcgcg cggcaaacca ccagtacatg gtgccgccaa ccagattcga cacgtaccag
    20281 gcaataacaa aacccacaaa accaaagtca aaataccagg ctgcgacgct ccccgctgcg
    20341 cgcagaaaag gtttcgtcgc ctgctgtaca gcaatcaaat caaagcgatc taccgcacgc
    20401 agaatgccgg tcggcgtgga ggaagccatt gaaggaatga gcgtgcaata cagcgccgcc
    20461 agccaaaagc tttggtcatc cagacctaat gaatgggaaa ggaatggcag taaggcaatg
    20521 ccgccgacaa tcgccaccgc gccgctgacg atatccagcg agaaggaaaa tgagacgaca
    20581 ttgcgaaatt gctgcggatt attgttggtc aaggctggcg ttccgtactg aaccaccagc
    20641 tgccatgtct gaaacttaat aaaatcgctg atcgacttgg cgtacgattg cacaatcacc
    20701 agtacaccaa acatggcggg cgtcatccct ttaccggcac acgagagcgc caacagcccc
    20761 agcaaggcgc tctcgacatt actggagcct aaccaggcgc tattgcgaat aatggtgcga
    20821 aacgcgccat ctgcaaacca atgtttgatg ttaaaacccg ccaattcagc ctgaccttat
    20881 cgttgatagt aaaaaatatc ccgccagcct taagttaaac ttcggcggtc agaaacgatg
    20941 gcaaccagag aaaccgcctt ctgtacctgt tccagcactt cgctgtaggg cgctctggaa
    21001 tcaatctcaa gaatttttgt gccgttatag ccaatcgtcg acatgacgcc gattttgtcc
    21061 tgcagctcgg catactcatg gtcaggcttg cgggagatgg cagtatcaat atcaatcccc
    21121 aaacgaatga ttaattcagg acggtattgc gccatttttt ggtataaact ccgttcgcgc
    21181 tgcgccagaa acatgctgat tttcccggtc gcacgttcga cgccgatccc cggtccgtca
    21241 taataaaagc ccgaaatttc agcctgcggg aagcgatcgc tgaccaccag aacgccactt
    21301 tgcgccagtc gctgaacctt gcgtagattc gccattcttc gcagcgagaa gcagtacata
    21361 atcaccgccg cccatagcgc cggagatttg gttttcatgc tttgggtttt cgaggatttg
    21421 gccgccaatc gccgttccag ccagacgcca accaacggca atcgtttgat tttgtcgccg
    21481 tcttcgccgg agagcagccc cagatagcgg cgctcggttt gccagtgttg ttgcagcgat
    21541 ttcaccaggt cggtggtcag cgtggattta ccggtgccat cacacccgac caccgcaatc
    21601 agccccggaa tgtagttggg ctgcggcgcg gttgtactat taacagtttg tgtttgtagt
    21661 gcatccattc gttaaatcat cccctatcca ttttggtgat caggaaagcg cggcgtgaat
    21721 tgcgcgtagt gacgcttgca taatttcatc ggctggttgc cgaccatcca gttcaaggat
    21781 cttggcgcca ttgaatgtca gttgcggcgt aacggcgatt ttttcctgta acgctgccag
    21841 ttggtggtca ggtttacgcg caaacgcggt ttgttcatca atgccaagac gaatcaacaa
    21901 tacgggtaaa taagacgcca tccattggta cagcttcaac tcgcgctgcc ttaacatttt
    21961 tatccaaccg ttaccgcccg tggtttttgc caattgcggg ccatcaaagc gaaaacccgg
    22021 cacttcaacc tgcgggtagc ggtcggtgat gagcagatag ccttgctggc ttttacacaa
    22081 cattttgcga aacttgtacg cccgccagca ggaaagcaga tagataacca gcgcagtaat
    22141 attgccaggc ggagttaagg gcttttcgtg tacatgtgcc gctttacttc gcagataaca
    22201 cccacaaggt gcgccaataa cagggagctg tgaaatccat tcgccaattc gcccggacga
    22261 ttgcccgaga taaatgtgtt ctgttggcat tcttgctgcc agttcattta ccaggcttgc
    22321 cgtgagggtc gatttacctg aaccatcaca tcctacaatg gcaataacac gcactggcgt
    22381 ggaattaatt attgacatat tacgttgatt cacgaaaaac ccggcagtaa ataatgtatt
    22441 gaatattacg tggtccgtgc tcagaatatc cgttcaacct tattgtgaca atgactaatt
    22501 gtgacattga cctgaattac gctttcataa aaacatatta accaaataaa tatttttaat
    22561 ggatatttaa attaaaggat atattcatgc agtcaataac acctccatta attgccgtta
    22621 ttggtagcga tgcttcaggc aagtcaacgg tgtgtgaaca tcttattacc gttgtcgaaa
    22681 aatatggtgc tgccgaaaga gttcatttag gaaaacaggc cggaaatgtc ggtcgtgcag
    22741 tgacaaaatt acctttgatg ggaaaatcct tacataaaac aattgaacga aatcaggtga
    22801 aaacagcaaa aaaattgcct ggaccagttc cggcgctggt aattacagcg tttgtcgccc
    22861 gtcgcttact gcgctttcgt catatgcttg cctgtcgtcg tcgcgggtta attgttctaa
    22921 ccgaccgtta tcctcaggac caaattcctg gcgcttacga tggtacggtg ttcccaccta
    22981 acgttgaagg tggtcgtttt gtctcatggc tggcaagcca ggaacgtaaa gcgtttcact
    23041 ggatggcgag ccataagcct gatctggtca tcaaactcaa tgttgacctt gaagttgcct
    23101 gtgcacgtaa acccgaccat aaacgggaat cgctggcgag gaagattgcc ataacgccac
    23161 agttaacctt tggtggtgca caactggttg atatcgatgc caatcagcca ctggaacagg
    23221 tgttggttga tgcagaaaaa gcgattacgg attttatgac cgcgcgtggt tatcactagt
    23281 caaaaatgga aatgcccgat cgccaggacc gggcattttc aggaaggtta aatcaactgc
    23341 aatgctatcc agtacagccc accagaaaga aaaattgccg ccggtaaagt aaacacccac
    23401 gccatcagga tgctggttac cgttttacgc tgtaacccac cgccgtccac caccatcgtc
    23461 cctgctaccg cagacgagag gacgtgtgtt gtggagacag gcatcccaat ataactggca
    23521 aggccgatag acactgccgc cgtcatttgt gccgccatgc cttgcgcata cgtcatgccg
    23581 cgcttaccaa tcttctcacc gatggtcatc gctacacgac gccagccaat catggtgcca
    23641 atgccgagcg ccagtgctac cgccatgatg atccacaccg gagcgtactc aatggtgctt
    23701 aacatatcgc tgcgcagttt tttcagcagg ttctggtctt ctttactgac gcctggcagt
    23761 ttcgccagct tcgcggaggt atcggagatg cacagcataa tgcggcgcag ctggctgcgc
    23821 tgactcacgc ttaacggctc gtaactttcc atattgcctg gcagcatcgt tttaacgcgc
    23881 gcaatcgcat caaaggtatt tgccggatga cagtgaaact ctgttacttg cgtgccatca
    23941 gtcgatgctg caggcaatgg aggttccatc gcaatcaact tctgcggcag ttcaggatgc
    24001 tgttgcaggt agtgttcgaa gttggtaacg gcatcgcggg tacgggtaat ttcatagccg
    24061 gacgcattca tattgacgac gaagccagca ggggcaatcc ccaccagtac cagcattacc
    24121 aggccgatcc ctttctgccc gtcgttcgcg ccgtgcgaaa acgccacgcc cgcagcggaa
    24181 acaatcagcg caatacgcgt ccagaatggc ggtttacgtt tgcccttttt ctttttgcga
    24241 tcttccggaa tgcggtgaat acggtcacgc tttttcgtcc cactccagaa acggcgcagc
    24301 aggaaaatca gaccacccgc aatgaccagg ccgacgatag gggaaacaat cagcgaggag
    24361 aaaattttgg ttacttcacg caggtttaac gcatccatca ccgatgagcc ggttaacagc
    24421 gcgttggtta aaccgatgcc gataatcgca ccaatcaagg tgtgcgaact ggaggccggt
    24481 aaaccaaaga accacgttcc caggttccag ataatcgccg ccagcagcat ggaaaagacc
    24541 atcgccaaac cgtgagttga ccccatattc agcaacaaat cgattggtaa catatggaca
    24601 atggcatagg caacgctaag tccgcccaat aacacgccaa aaaagttaaa aaatgccgcc
    24661 atcaccacag caagttgtgg ctgcatggca cgagtataaa taacggttgc caccgcattc
    24721 gccgtgtcat gaaaaccatt gattgcttcg tagaacaaca caaatgccag agcaagtaat
    24781 aacaaaagcc ccgtatatat atcaaggcca acaaataaat ttagcatata ttagattacg
    24841 ccattttgaa tttacgaacg gacgcattat cagtgacttt aacggcatgg gcaaagtgaa
    24901 atatcatttt ttaacgataa aaagagtgtt atttcttgtg tatcagacaa taagcattca
    24961 ttcaaaacct ttagaaataa tcaaaacaca ggtattccac tggttaatat attcacatat
    25021 gaaatgaata aatattggaa tatataaata ttgaatattt tgattaatcc ccgtactaat
    25081 tattcttcat aatcagtacg gggttgctac aacatgtatt actttttcac cacaattaac
    25141 ggttcaatat cactctcttt tttgatgacc agtgattcat caccgcgcaa acacgtccca
    25201 ccgtagttgc cgccaacggt gaaggtacat acctgaatgt atttaccgtc cactttcggc
    25261 aaacaccaca gttgctgata gatgtttttc tgctcggcaa atttaccgct ggttttgtcc
    25321 agcacctctt catgatggct gaccaggtcg atattgctac cacagcgacc ggcgatcggt
    25381 ttcactgcgt aacctgtttt caccaattca tcattaacag tgaaatcggt atccagcagg
    25441 taacgatggt gcgggaacag cgaccagagg atcggcagaa tcgctttgtt gccggggatc
    25501 accgtccaca gcggctcaaa gaccagcact tccgggcgca gcaatacgtc gataagacgc
    25561 acttcgtttt gcggatgacc ggtacggatt ggcaccgcag caaactcacg gtcgctaact
    25621 tcacgaatct gatcaaacgc ggtttcccac gcccaggttt tccacacgca gttaaccagt
    25681 cgcccttccc catcaatcag ttgcccggca gcatcccagc ccagttcatc cagcccgcgc
    25741 aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct ccataaactg cgcgtgatag
    25801 ttttcctcga tatctttgtc ctgcatgata tggacaaacg gacgtgcacg actgtgtttc
    25861 caggcaccag ccagttcgtt aatcagccct tccgccggat tgaagccgtt gcctttatag
    25921 ccctgctccg cccaacgttc gaggatcaag cccgcttcgg tatgacagga ggcggagtcg
    25981 gcgttgtatt cgtaaacctt caggccacgc tcatccatgc agaaatccat acgaccagtg
    26041 atcatatggt gacggcgacg ctgccaggag agacgcaaac gtggccagag gattttcgga
    26101 atgtcgaaca gcgccagcag gttgtcatct ttcagcacct tgtcggttgc gtgaagatac
    26161 atcagatgca gctcgttggt ggctttaatt agctcctgct cggcactctc ggtaatggtg
    26221 tagtaatgat acggatcctg attgatcacc tgaccgttgg cctgcacata ggcgttttgt
    26281 agcggatctt tttcatccag ccatttaccg tcaaactggc ctttgttttc cagacgcgct
    26341 ccactgattt tcagcagctc gcctgcaatt tccggctgcg gtaagctgta ttcagtatct
    26401 tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt aagggtatag
    26461 cagccgtttt cgaccaccat ctccagctcg cgcgtccact gttgcccttg cggcaacggg
    26521 gaatgaatca cgttctgttc cgcaatacgg actttgttgc catgcaattg ggtaatgatg
    26581 gcgacatggc cagtgtcttt aaattcaccg cctttatccc agataagaag cgcacccgcg
    26641 accggcgcac gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt gtcattaacc
    26701 acttctcgca ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc agtaaagacc
    26761 acaccgtaat tcagaaagag aaaacggcga gcaaattcaa cgcattgcca cttgtggccc
    26821 atatattcgt cgtcgatata gctacggaat acggcgtcat cttcgtattc ctgcggatcg
    26881 agagaactgt aatctgaaga gtagattgct accccacctg gggcgtagcc caataatgtc
    26941 ccgaacgggg catcctggct ggtcgttcct ttgctcatca ctttaccttt aacaatacaa
    27001 cctaagcagt tggcgtaatt gttgtgctct gattacctgc tcagcgacat taaccggaca
    27061 gaggtcagac taatcataca cctcatcgca gcggctggcg caaagggtta aaaaattcac
    27121 attctgtaca gcaagtgacc tgctacactg cttcaacact accactcaga aggcaactca
    27181 ctatgacaga caatacttat cagcccgcga aagtctggac gtgggataaa tccgctggcg
    27241 gcgcgttcgc caatatcaat cgcccgtttt ctggtccgac gcatgaaaaa acgctgcccg
    27301 ttggcaaaca tccattgcaa ctttattcgc tgggaacgcc gaacggtcag aaagtaacga
    27361 ttatgcttga ggagctactg gcgctgggcg ttactggtgc agagtacgac gcctggctga
    27421 ttcgtattgg cgatggcgat caattctcca gcggctttgt cgaagtgaac ccaaactcga
    27481 agatcccggc gctgcgcgat catacgcata atccgccgat ccgcgtgttt gaatctggtt
    27541 cgatcctgct ttatctggcg gagaaatttg gctacttcct gccgcaggat ctggcaaaac
    27601 ggactgaaac gttgaactgg ctgttctggt tacagggtgc cgcgccgttc ctcggcggtg
    27661 gttttggtca cttttaccat tacgcgccgg tgaaaattga gtacgccatc aaccgcttta
    27721 ccatggaagc caagcgcctg ctcgacgtgc tggataagca actggcgcag cataagtttg
    27781 ttgcgggcga tgagtacacc attgcggata tggcgatttg gccgtggttt ggcaacgtgg
    27841 tgctcggcgg cgtgtatgat gccgcagagt ttctggatgc gggaagttat aaacatgtgc
    27901 aacgctgggc gaaagaagtg ggcgaacgtc cggcagtgaa acgtgggcgt attgttaacc
    27961 gcaccaacgg gccgctgaac gagcagttgc atgagcgcca tgacgccagt gattttgaga
    28021 caaatacaga agataagcgt caggggtaag ggttggtgtt cgtcgcagca agccatccag
    28081 gccggataag gcgttcacgc cgcatccggc aatcgtgcac aatgcctgat gcgacgctgc
    28141 cgcgtcttat caggcctaca aaagctaatc atcacgcgct ggtaatgtcg taatccattt
    28201 ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc gatgatgctc atggcaaatc
    28261 cgacgtgtac cagcacccac tggcccagca gatcggcagg gttaccttcg caaatcaggg
    28321 cgatattcac gtcgcgcttg ataccacata cttcaacctg cgcaagctgg tgaatatctt
    28381 caccgacagc cagcacctgg cctggaacgc caatacacat tgttaactcc ggttatttcg
    28441 attgccggac aaacgcgaaa aagaaccttt agcgggtgtt ccctctcacc ctaaccctct
    28501 ccccggaggg gcgaggggac cgtccgtgcg ctttttaggc gttgtcctca gtcttattca
    28561 acttcaatac ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga
    28621 cagagcggac actgcgcatc gtgctgatga atctccacca cctggctgca atcccagcac
    28681 caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg
    28741 cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcgccaatt
    28801 tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt
    28861 tcaacggcgc tctggcaaag cgacaactca tgcattttcg cccctcctgc ggccaaacag
    28921 cagggcgcga cgccctgcgt gtggtacatc cggattggtg actggcaacg aaaggatcat
    28981 tcgtgcgcag tcatccgtca ggcgttggcc ttcttcaatc gacatgctgt gcgaaagcgg
    29041 cgacatcagc gaacaggaga ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt
    29101 catggtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag
    29161 ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt
    29221 ccactgacct tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa
    29281 agagagatcg tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt
    29341 cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt
    29401 ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca atcattgctt
    29461 caaccgtcgg cgttaagccg atgtgtggct ccagcgattc cgggatcacg ccgaccaggg
    29521 tcagcttttt cggaaactca ccggtgaagc gcagggccga caatacgtcg gccaggccaa
    29581 gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca
    29641 tcatcgttcc cggagtgttc tttttcgaca caatggcatc cgcaataatc agatgatcgc
    29701 gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat
    29761 aatccggcag aatgtatcgt tgctctaacg cttcgacaat ccgcacgccg atggcttcat
    29821 cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga
    29881 aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa
    29941 ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcggcaaccg gtgtacccac
    30001 cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcggaccag agttccaggt
    30061 tgatggaaca accgcctggt agttgctgat gataccgtct ttaataacca tccagtgaga
    30121 gagcataccg cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat
    30181 gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta
    30241 ttggttttgc aggatatcct gcaattcaca gcagtgaacg gtacgaccaa taatacggcc
    30301 cagcgtggag tgcagctgtg ccacttccag cgtgttgcca gtcagtttct gataaatcgc
    30361 aacgatttca ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat
    30421 attagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga
    30481 atatttcccg tcgtcagacc aaccatcata agccggaatg gtggtacctt cccacggcgc
    30541 ctgtggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag
    30601 gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta
    30661 gcctcccggg aacaggaagc tgccgttttt gctgtcggtc gggaattccg gcacgctcag
    30721 gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcgg cgataaccgc
    30781 ggtatcaacc ttataaacct gctcaacaaa gtcgctcagt ttgtcgatga aagacttgat
    30841 gtacatcagg cgctcaaggt tcaacacgcc caacccgtcg aggttgattg ggttcgcgac
    30901 accacccaca gccaggttct gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg
    30961 gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac
    31021 ttccggcggc agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg
    31081 accgctggca accagatctt tgatcttgtt ctgaacttta gtgaactctt ccggactgtt
    31141 cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt cagcttgcag
    31201 tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc
    31261 atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc
    31321 gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac
    31381 gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc
    31441 ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat
    31501 ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg
    31561 gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc
    31621 ggcagtagac gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa
    31681 atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc
    31741 aacgaatagg tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac
    31801 agcatacggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc
    31861 agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc
    31921 ttgtcgcgat agataagctc gccaaagcgc agcccgatga aaatcgccag caacacactg
    31981 atggtgttgg tcagcttgac gaacaggctc ttttcatccg gaccgttgcc acgcagaccc
    32041 gcctgcacca gtgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc
    32101 agcgagaaca gcggcaacat ttcatagctc tgccagagcg gatgcacctt gtagcccgcc
    32161 gagatcatca gcgaccccat tgaagactgg tgcatggtcg gcagcagcgc accgagcgcg
    32221 atgatgaaga acatcacctt gttcagacgt tgtagcgaca ccttccagcc cagacgttca
    32281 aacagtgccg gagcaaactc cagagccatc acgccgatgt agatggtcat acagaccgcc
    32341 gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc
    32401 cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc
    32461 gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc
    32521 cacgccagcg cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc
    32581 cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga
    32641 cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta
    32701 ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg
    32761 atggtcgttt ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg
    32821 tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag
    32881 atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg
    32941 agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag
    33001 gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag
    33061 cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc
    33121 cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt
    33181 ctggttgcac agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg
    33241 cacgttgtac ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta
    33301 atggacaatg ccggttttcg gatctttttt cagtgcagag accgggcaca cagagacaca
    33361 gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg
    33421 gtctttgttg acccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga
    33481 cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat
    33541 atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat
    33601 ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag
    33661 cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt
    33721 tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc
    33781 atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca
    33841 gagacgttgc cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt
    33901 tcgacgttgg caagctgatg gatgccttta tggaagccga tacctttttc gttacagcca
    33961 tagcacgggt gaccaatcgc caccggccac acaccgccaa catcgcagaa ttgcagcgtt
    34021 gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaagca ccagccttcg
    34081 cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt
    34141 tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt
    34201 ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc
    34261 ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg caagctgact
    34321 gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcggagca ggaaccaata
    34381 gcgataatgg ctgctgagcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg
    34441 gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca
    34501 tactgccctt tgtacttctc gagagcgtta tgtttgttct cttcgacctg atgaccgaag
    34561 gcggcggaaa gcacttcgtg atattccaga gagatagtct ccagcacgag gttttctacc
    34621 gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc
    34681 cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg
    34741 ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta
    34801 atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaga
    34861 acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc
    34921 acacatttat tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa
    34981 gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc
    35041 gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca
    35101 ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa
    35161 gctggcgatc tccggtcttt tcgtacatcc tgtgtgagta gcctaataca aaaccgcgtt
    35221 tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc
    35281 ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcat tatatatcgc
    35341 catatataac gatattttag gcaaagaacg acttttgcgc acgctatttt ttcatacttg
    35401 tttaagacgt aatcccgtaa gagataaacc tatcaggaga gatatttatc gaaccatttc
    35461 agtgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta
    35521 tggaatccgt gattaacccc cggatagata taagcctcat aaaccttatt attgtctttc
    35581 aacgccgcct cgtaaggagg ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg
    35641 agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac
    35701 ggcaccgcgc aggccagttc cggatacgcg acagccgccg cgttcgatac gccaccgcca
    35761 tagcaaaatc cggtaatacc cactttacct gtcgcttgcg gatagcgttg cataaactca
    35821 attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga
    35881 cctttatcat catttcccgg ataacctcca acggaactta atccgtcagg tgccagggcg
    35941 atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt cagtccacga
    36001 ttctcatgca ccacccccac ggctggcgtt ttgccgctca tttttgcggg tttcaccaga
    36061 taaccccgta cctcgccgtg accatttggc gaaggatacg tgatgtactc agcaacaatc
    36121 tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac
    36181 aacgctaatg ccgtcatccc gcccaccgca tacttcgccg caaggttgag gaactcacgt
    36241 ttcgaaattt tcccgtgaac gtaatagtcg tagtaatcca acaactcttg tgggaaatct
    36301 ttggcggtta aacgcggcat cgttgcattc ctcaactggt gtttttttaa gcaaagcata
    36361 agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg
    36421 gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga gaggtcatgg
    36481 tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgc ggaaaaagtg
    36541 gtttacgcct gcccgcatta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc
    36601 tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt aggcattacg cactttgatt
    36661 tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc
    36721 gcgaggattt tgctgcttat cgcgatgaac tgattatctc taccaaggct ggctacgata
    36781 tgtggcccgg cccgtacggc tctggcggtt cacgtaaata cctgctcgcc agcctcgacc
    36841 aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg
    36901 aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg
    36961 cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg gtcgagttgc
    37021 tgcacgagtg gaaaattccg cttttaattc atcaaccttc gtacaattta ctgaaccgct
    37081 gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgtattgcct
    37141 ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt ccgcaagatt
    37201 cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg cttaccgaag
    37261 ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga caatcaatgg
    37321 cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta ttgattggtg
    37381 ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct gaataatctg acatttagca
    37441 ccgaggagct ggcgcagatt gatcagcata tcgccgatgg cgagctgaat ctgtggcagg
    37501 cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga atcagatcag
    37561 tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagaaag cacaaacgtc
    37621 agatggataa tgacgtacca catcagtttg ttatccggga catttttcgc atccataaag
    37681 acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt gtttttcagc
    37741 gacgttgcgt ccattttccc cagccacttc agcttctctt tgttctcgga gatatccagc
    37801 tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc gccaaccagc
    37861 gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat cgagaagata
    37921 ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc
    37981 gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga
    38041 aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattgagt
    38101 aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata attctgaggt
    38161 ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt
    38221 gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca gtattacact
    38281 ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat gacaccgata
    38341 ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagcactg
    38401 gtgacagggg gcgattccgg aataggtcgc gctgccgcca tcgcttacgc gcgtgaaggg
    38461 gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag
    38521 atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa
    38581 ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag gcgggctgga tattatggcg
    38641 ctggtcgccg ggaaacaggt tgccattccg gatattgcag acctcaccag cgaacagttt
    38701 caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga agcgatcccc
    38761 ctgctaccga aaggtgcaag tgtcatcacc acttcgtcaa tccaggcata ccagccaagt
    38821 ccgcatttac tggactatgc ggctacgaag gcggcgattc tgaactacag ccgtggcctg
    38881 gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg ccctatctgg
    38941 acggcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt tggtcagaaa
    39001 acgccgatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt ttatctggca
    39061 agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg cgagcattta
    39121 ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt cactcttacc
    39181 tgatgtggcg aaaacgcctt atccgcctac aaacgcatga agattcaaca tgttgcaata
    39241 gattttgtag gcctgataag cgtagcgcat caggcattga gcacctaatg ccggatgcgt
    39301 cgtgaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca atagattttg
    39361 taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg gcggtttctt
    39421 cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt accttcatca
    39481 acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct ttcttgcctt
    39541 cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg ttaccgataa
    39601 acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc ggctgcggcg
    39661 tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt gccgccacca
    39721 taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg atatcatgca
    39781 tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat tatcctgcgc
    39841 gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga tctaggtcac
    39901 ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta aagccgccaa
    39961 tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg aggccgattg
    40021 ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta gtggtctgcg
    40081 tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac agaccaacga
    40141 acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt tgacgaccca
    40201 ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct tcgttatcgt
    40261 cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc aggcttaagc
    40321 ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgt gcttcagcca
    40381 gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg ctcttactga
    40441 agaagattgc ccaggtgact acagaggcca aaataagccc aatcatcacg cacttaacga
    40501 caatatcggc gtactgatac ataccccaga cggaaaggtc cgtctgcatt aaattattac
    40561 ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat caaaacgacg
    40621 tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc tttcttttcc
    40681 ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt ctgctttatg
    40741 ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca gacgtataaa
    40801 aacaggaatc ccgacatggc ggacaaaaag cttgatactc aactggtgaa tgcaggacgc
    40861 agcaaaaaat acactcttgg cgcggtaaat agcgtgattc agcgcgcttc ttcgctggta
    40921 tttgagagta tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg cgagttgttc
    40981 tatggacggc gcggaacgtt aacccatttt tccttacaac aagcgatgtg tgaactggaa
    41041 ggtggcgcag gctgcgcgct atttccctgc ggggcggcag cggttgctaa ttcgattctt
    41101 gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga accgagtcag
    41161 gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt tgatccgctg
    41221 attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt tctggaatcg
    41281 ccgggttcca tcactatgga agtccacgac gttccggcga ttgttgccgc cgtgcgcagt
    41341 gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt gctgtttaag
    41401 gcgctggatt ttggcatcga tgtttctatt caggccgcca ccaaatatct ggttgggcat
    41461 tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca gctacgggaa
    41521 aatgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac cagccgtggc
    41581 ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa agtggccgaa
    41641 tggctggcag aacatccgca agttgcgcga gttaaccacc ctgccctgcc tggcagtaaa
    41701 ggacacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc ctttgtgctt
    41761 aagaaaaaac tcaatgatga agagctggcg aactatctgg ataacttcag tttattcagc
    41821 atggcctact cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc agaacatatc
    41881 gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg cctgcatatt
    41941 ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc acggattgta
    42001 taacattgcc acttttggac aattttgcag acattttatt gtgaaaagtc ttaaattgtt
    42061 gcgtccggga tcaaggcgtc ccggacgatt caggagtaca ataggcagat aaaggcttaa
    42121 acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg cgctctggca
    42181 acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact ttgtcatgtt
    42241 tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag gcgacagctt
    42301 gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc ctacgattgc
    42361 gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg ggagctggtt
    42421 agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat atcaccagcg
    42481 cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac gttttcttgc
    42541 atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa accgccgctt
    42601 ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca gttttggcta
    42661 tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga cgttcctgat
    42721 gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg tggtgattaa
    42781 aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac cgggaacgta
    42841 tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc acaccgaagt
    42901 aacgtttaaa ctcacggcta aattgcgatg cgctttcata gccgacgcgc atcgctgctg
    42961 cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga cggtaattct
    43021 tcaaatactg caacggcgag gtgctggtga cagacttaaa attatggtgg aacgccgata
    43081 cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg tatttattct
    43141 caattcgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg accagcgcca
    43201 gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg atgatctgtt
    43261 tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc tccgccgcgc
    43321 ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc gacggctgga
    43381 aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata tcgacattga
    43441 gacgcaaccc tgccagcggc acctctgacg tggcataggt ttcgcactca aacggcaacg
    43501 gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca ttgatataac
    43561 cgattttatg cccggaaaag agaattatga tgccaggctc gtacatcacc ggtgtacgtg
    43621 cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac aaactatttt
    43681 ctttattttt cagtttatta actttatccg ccagcaagcg acaaatctct tcacgtttca
    43741 tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt tgtagcattt
    43801 ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga gagccagggc
    43861 gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata ttggcccagc
    43921 aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt ctgtttggta
    43981 aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta ttgattacct
    44041 acggtggcgg cagcgtgaaa aaaaccggcg ttctcgatca agttctggat gccctgaaag
    44101 gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa acgctgatga
    44161 acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt ggcggcggtt
    44221 ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa aatatcgatc
    44281 cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg atgggctgtg
    44341 tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc tcccgtaaaa
    44401 ccacaggcga caagcaggcg ttccattctg cacatgttca gccggtattt gccgtgctcg
    44461 atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta gtggacgcct
    44521 ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt caggaccgtt
    44581 tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg aaagagccag
    44641 aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg aacggtttga
    44701 ttggcactgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa ctgactgcga
    44761 tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg tggaatgaaa
    44821 aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg aacatcactg
    44881 aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat ttctttgagc
    44941 aattaggcgt gccgacccac ctctccgact acggtctgga cggcagctcc atcccggctt
    45001 tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat gacattacgt
    45061 tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc tcaaactttc
    45121 gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc tgctccggtg
    45181 agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac aggatggcaa
    45241 tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag taatcaccgc
    45301 cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg cctacaagaa
    45361 cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag aactgttcat
    45421 caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc tcgacagcct
    45481 gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg ttcccgctat
    45541 cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg gattaatcaa
    45601 aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg atgaaactgg
    45661 cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac gccagctaca
    45721 cgcttggaac gcgacacaca aaatccagac cgaatcctgg agcccattag cgcaaggagg
    45781 gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg gcaaaacccc
    45841 ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc cgaaatcggt
    45901 cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg acaaagacga
    45961 actcggcaaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc ctgaccagtt
    46021 cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg acttttcaga
    46081 aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag gaatgccaaa
    46141 tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat ctatatccgg
    46201 gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt ttagcagccc
    46261 tggcaagttt taccgtacac gcgcagcctc cttcacagac cgtagaacaa acagtccggc
    46321 agatttatca gaactataaa tcagatgcca gtacgcctta tttcggtgaa accggagagc
    46381 gggcgataac ttcagcgcgt attcaacagg cgcttaccct gaacgacaat cttacactac
    46441 cgggcaatat tggctggctg gattatgatc cggtttgtga ttgtcaggat tttggcgatc
    46501 tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga tcatgccgat gccgttgtgc
    46561 gctttcgtat ctttaaagat gataaagaaa agaccacaca gacactgaaa atggtggcgg
    46621 aaaatggtcg ttgggtcatt gatgatattg tcagcaatca tggcagcgtc ttacaagcag
    46681 ttaatagcga gaatgaaaaa acgctggccg ctttagcttc gttgcagaaa gaacagccag
    46741 aatcctttgt tgctgaactc tttgaacata ttgctgatta tagctggccg tggacgtggg
    46801 tggtttccga ctcttaccgc caggcggtta atgccttcta taaaaccacc ttcaagacgg
    46861 ccaataatcc cgatgaagat atgcaaatag aacggcaatt tatttacgac aatccgatct
    46921 gttttggcga agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg gagaaaaccg
    46981 ccgattccgc ccgcattcat gttcgtttta cgctgaccaa tggcaacaat gaagagcaag
    47041 agctggtttt acagcggcgc gaaggtaagt gggaaatcgc tgattttatc cgcccgaaca
    47101 gcggcagcct acttaagcag attgaggcaa aaaccgccgc cagattaaag caatgagctg
    47161 aattaaataa caattagccg gaacaataaa taaaagggaa cactatatga aaacgatttt
    47221 caccgtggga gctgttgttc tggcaacctg cttgctcagt ggctgcgtca atgagcaaaa
    47281 ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc aaaatcgccc ggcttgagca
    47341 ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa tccgaagcta acagagccaa
    47401 tacgcgtctt gatgcacagg actattttga ttgcctgcgc tgcttgcgta tgtacgcaga
    47461 atgataaaaa aatccccggc agcatgtcag ttgccgggga ttttttttaa cgtccaaccg
    47521 ccgctttagg gcgtttcttc gcaccagcat tcaccggacg agattgcgta gacgacgctt
    47581 tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg cgtatgttta gtcaacgccg
    47641 gacgggtatt gcggttctgg cgacgagctt cacgcatctc ttcaatggtt ggcgcaggca
    47701 ctaagcaatc gcgacggctg ccaatcagat gctttttgcc catcgcttcc agcgcctggc
    47761 ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct
    47821 gtttgtcgcc cttcggtacg aagacgcctt cgctcttata accaatcttc gccagcggtt
    47881 ttttcccggt gtaatacatg gtggttgagt tcgccagcgg cgatgggtag aagttctgta
    47941 cctggtcgag acggaaacga tgctttttca gccacagcgc caaattcacc atatcttcat
    48001 cacgcgtacc ggggtgcgca gagatgaaat acgggatcag atactgctct ttacctgcct
    48061 gtttcgagta ggtatcgaac agctctttaa agcggtcata gctgcccatg cccggcttca
    48121 tcatcttcga caacggccct tcttcggtat gttccggagc aatcttcaga taaccgccga
    48181 cgtgatgggt cgccaactct ttgatatagc gcggatcttc cacggcgatg tcataacgta
    48241 cgcctgaggc gataaggatt tttttaatgc ctttcagatc acgcgcacgg cgatagaggt
    48301 tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat atccggataa acgcacgaca
    48361 aacggcgaca agtttgttca gcgcgtggcg atttgcagcg caacatatac atgttggcag
    48421 ttggcccacc gagatcggaa atcacgcccg taaaacctgg aacggtgtcg cggatcgctt
    48481 cgatctcatt aatgatcgaa tcttccgaac ggctctgaat aatgcgccct tcgtgctcgg
    48541 taatagaaca gaaagaacag ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga
    48601 tcatttcgta agccggaata cgggcattgc cataggccgg atgtggcacg cgcttgtacg
    48661 gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc gggttgatcc
    48721 acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc gcagcctggg ttggtttcgt
    48781 ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc gcccttcact ttctcgaaag
    48841 aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg cggtggctgc acgattacag
    48901 ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc cgcacacggc aaatcttcgc
    48961 catacggatg cgggattggg tcgattttcc ctagggtatc aagacgggtg gaatccacac
    49021 cgctccagcc aggcagcgcc tcttttacga taattgcggt attacgcaca tcgcggattt
    49081 cactaatcgg ctcgcccatc gccagacgat gcgctacttc caccagcgga cgttcaccgt
    49141 taccaaacat cagcatgtcg gctttcgaat ctaccagaac ggaacggcgc acggtatcgg
    49201 accagtaatc ataatgcgcg gtacggcgca ggctagcctc aataccgccg aggatcaccg
    49261 gcacatcttt ccacgcctct ttacaacgct gggtataaac cagcgtggcg cgatccggtc
    49321 gcttacccgc gacattatct ggcgtgtagg catcatcatg acgtaaacgg cgatcggcgg
    49381 tatagcggtt gatcatcgaa tccatgttgc cagcagtaac accaaaaaac agattcggtt
    49441 tacccagacg cataaagtcg tctttgctgc tccagtccgg ctgggcgatg atcccgacgc
    49501 gaaagccctg cgcttccagc atacgaccgc aaatcgccat cccgaagctt gggtgatcga
    49561 catacgcgtc gccagtaacc aaaatgatgt cgcagctatc ccagccaagt tgatccatct
    49621 cttcacgaga catcggcaaa aacggtgccg gtccaaaaca ggcggcccag tactgcggcc
    49681 aggagaacag gtcgcgatcc ggttggatca gggagataga gctcattttg cttccagaaa
    49741 tgataaaaaa ataatcaaag gccggggatt ataagccgga acgaaagaga aatcgaaagg
    49801 tattccatac tcgccctcct cgggcgagta tgaagattac ggtaccggat tgaccaacag
    49861 ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt agaacgggaa
    49921 gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt caacccaaac
    49981 ggtatctttc cagcctctgt cttccgggaa cggcatcgcg ccattcacgt tacggatctg
    50041 gaacattacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc
    50101 ccacgttcct tgctgcgcgg tgacatcaat acggttgacg tcccacagct gtccattaat
    50161 acccgggtca tcacccagac tgatatcgcg actgcgaatt ggcgaaccag ccatgatttc
    50221 agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg
    50281 cagcgttagc accaaggtag aaaccagaat actggatggc tcaaagaagc cgcgaatacg
    50341 atcaacaatg ctcgccgctt caccacaggt gatcgacact tcatcgccgt tgctcatatc
    50401 caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc
    50461 aggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc tcatctgtag
    50521 ttgatagcga cgagagttcg acgcgttcag cagtcgcaag cgcacccagc cacgcgagac
    50581 ttcaacgtac gggctttgta caccgttaac cagcagcgta tcaccaacaa agccgccgct
    50641 tcccggttcg ttgtattctg gcgtaccaaa gttatccagc cgtttatcct ggataatgac
    50701 cggaaaatca tccacaccat aatggttggg gataggcagc gacttgctga cttcatcttc
    50761 caccagccac attccggcaa ggccgttata gacctgctga gccgtgcggt tgggagtatt
    50821 ggcgtgatac cacagagtag ctgcgttctg gcgaataggc agtacgggtg cccagtcagc
    50881 gtttggcgac atcatccgtg ccggaccgcc catcagcggg cctggtacct gtagcccggc
    50941 caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc
    51001 cttccagacg cggatagtcg gccccaggta acgaccattg attccccaga ccgacgcgcg
    51061 tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg
    51121 gcgagattca agtagcggcg gaacgggtag cggttgctgt tgcccggctg cgctggcctt
    51181 caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact gacgccgact
    51241 gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc
    51301 ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc tgcgacttct
    51361 ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc cagctcacga
    51421 acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac aatcaccaga
    51481 ccgttgtgca gtcgattaag attaatctta ttcgaagttg tagagacgca cacgggaata
    51541 atcgggacgc ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg tagcaggccg
    51601 cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg ttttttgaaa
    51661 tgattcacga cttccgcaat ggtgccgtga gctttagtgc gattgtttct gtcgatcaat
    51721 aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa gctcttttta
    51781 cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc
    51841 tggtggttag cgatatagat agcattgccg tagctttcag catctgcagg tttgcggcac
    51901 tcaactttca ggccgaacag tggcgcaaga cggccaaaca tatgcccaaa ggtggccaca
    51961 tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca gactaagatg
    52021 ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc tgaaggtctg
    52081 agagactggc attgtacgtc acctgaaatc aggttagggc attgttaaaa gccgctctct
    52141 ggaaaagcgt attgttaatc gcaacgcatg aataaacaac ggtttcaagg gaatttttat
    52201 cgtttccggg ggttgaatac gctgccggat gacgacttaa cgtttcatcc ggcgttcctt
    52261 gctagcggga ggaaacagcg ccctccccgg catattactc ttcgctatca ccgctgctgg
    52321 cacggcgagg agagtcgatc tcaacacgat cgatacgctg caaaccgcgc atcaacgtac
    52381 cgcggcgtcc acgttcgcca gtgactttct gtagctcttc cggacgcagt ttaattttgc
    52441 gtttcccaac atgaatggtc agcgtgcttt gcggcggcag aacgtacagt tgcgccagac
    52501 catcctcgcc acgcgcggct tctgccgatg gaatgttgat aatcttgttg cctttgccct
    52561 tcgacagctg cggcagatcg cttaccggga acatcaacat acggcctgcc tgagtgattg
    52621 ccagcagcat atcggaagca tcttcaatca ccaccggcgg cataacatgg gcattttccg
    52681 gtaaggtgat caaagcctta cccgcacggt tacgcgctac cagatcgtta aaggtgcaga
    52741 cgaaaccgta acccgcatcg gaagccatca gcagtttctg atcgtcgctt tccatcagca
    52801 tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt tcgccctgac
    52861 cacgcgccga cggcagcgta atcgggtcga tggcatagct acgaccggtg gaatcaacaa
    52921 acactaccgg ttggttactc ttacctttca ccgccgcttt gaagctatca cccgctttat
    52981 aattcaggcc cggcgcgtcg atatcatggc ctttagcgct gcgtacccag cccatctgcg
    53041 acagcacaat ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt
    53101 cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca
    53161 gttctttctt cagcaggtta ttcattttac gctcggaagc caaaatgccc tgcaactggt
    53221 cgcgctcttt ttccagctca ctctgctcac cgcgaatctt catctcttcc agtttggcaa
    53281 gatgacgcag tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg
    53341 acatcagcgc cggtttcggt tcatcttcgt tacgaatgat ctcaatcact tcgtcgatgt
    53401 tgagaaacgc caccagcaaa ccttccagga tatgcaggcg cttgaggact ttctccagac
    53461 gatagttcag tcggcggcgc acggtatcgc gacggaacac cagccattcg gagaggattt
    53521 ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat catattgagg ttaatgcgat
    53581 agctcttttc cagatcggtg gtagcgaaga ggtggttcat cacctgatcc atatccacgc
    53641 ggttggaacg cggcacaatc accagacggg tcgggttctc gtggtcagat tcatcgcgca
    53701 gatcgtcaac catcggcagc tttttgttgc gcatttgcgc agcaatttgc tccagtacgc
    53761 gcgcacctga aacctgatgc ggtaatgcgc tgataaccac cgcgccatct tctttcttcc
    53821 acaccgcgcg catacgcact gaaccacgtc cgttctcgta gattttacga atctcggcgc
    53881 gcgaagtgat aatttccgct tcagtcggat aatccggccc ctgcacgata tccagcagct
    53941 gatcgagcgt agttttcggc tggtcgatta atgcgattgc cgcctgagct acttcacgca
    54001 ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat accggtggtg ccgttaagca
    54061 aaatgtttgg cagacgggca ggtagcattt tcggctcctg caaagtgccg tcgaagtttg
    54121 gcacccagtc agccgtcccc tgccccagct cgctcaatag cagctcggaa tatttcgaca
    54181 accgggattc ggtgtaacgc attgccgcga acgatttcgg atcgtccggc gcgccccagt
    54241 tcccctgacc atcaaccagc ggataacggt aagagaacgg ctgcgccatc aggaccatcg
    54301 cttcataaca ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca ccgacggtac
    54361 gggccgattt tttaaatttg gcgctggcat tcaggcccag ttcagacatc gcatacacaa
    54421 tgcggcgctg aacaggtttc agaccatcac caataaacgg caacgcacgg tccatgatca
    54481 cgtacatgga gtagtttaag taggcgtttt ccgtaaattc atgtagcgca aggcgctctg
    54541 ccatatcgct cattaattct gattcctcaa cttattcgcc agccttcaaa ggggcaatat
    54601 tgccggagat actacattat ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc
    54661 gccggacgtg ctccgacggc gataaaggca tcaatgtgcc ttgatataca actctttgct
    54721 gtagacttga tcggcataga gggcagcaca aggttgcgcc catgccacac cactcggcat
    54781 gggggtccgc tccgggcgat tcgagaagtt gaggttatgc gagtccaggg aagcctaaag
    54841 catcaaagta gtcgactgta agtatacggt tcagcccagt ttagctgtgt ctccaccagt
    54901 gccccgcgcc tcctgccata agtgcggctt gctcatggat atccccgaga cggcttacgg
    54961 cgcaccggat aataaatttc gcgtatacat ggtattcctt accggagaaa ttataagccc
    55021 aacaacaata cgcctgtgac attgttcgct caaacttatc gcgagtgatt tggctcacac
    55081 tccggtaaag attccccatc acgccaacgg cacataaaca tccgtttcca gtcgctgctc
    55141 tgtcacccct tcggcaagat tggtgtagtg aaacagaatc ggtgctttac gcattttctc
    55201 gccgcttgca ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag
    55261 ctcgccaacg tggcgggcca cggcataacg tccaccggta agttcaccat tgctcacacc
    55321 ataacgatta tcgggaatcg gttcgctaac gctgccgcag atatcaaagc gaaacgcttc
    55381 cggtgcggtg gtggcagggg cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg
    55441 agaaagcccc gtctctttac gccacatgat aaatttcgcc acactgtagt tgagcaactc
    55501 agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga taatgtttac
    55561 atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg
    55621 ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt tcaacagaat
    55681 tccagtcttt atcgacttca ccctgaatct caaccgtatc tttcggcgtc accgtcacac
    55741 cgttccagcg tttgtgatcg atatcaacat tgatagtacc gctggcatct ttaaacacgt
    55801 agagatcgtc agagatgcgt tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac
    55861 gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctga aatcctccgg
    55921 cctgactttg cgttaccgat gggccagaaa aaccgccctg ctctgctgcc atcaccggtg
    55981 cactgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt attactccct
    56041 ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta agggaaaaaa
    56101 ataaaattta atgctgtaca gagcgcgtta caacacggtt tactggcagc aaatacggtt
    56161 atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg attggtgacg
    56221 gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga ctggtttaca caaggtcgtc
    56281 agggaaaaga ggcgctttat agcgcacctt atgatgcggt gatcctggat ttaaccttac
    56341 caggcatgga tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag cgtgagccgg
    56401 tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg cgtctgggag
    56461 ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg ctggaggctc
    56521 tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggtaac gtcatgctcg
    56581 accccggcaa acgtatcgcc acgctggctg gcgaaccctt aacactgaaa ccaaaagaat
    56641 ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc aaactgattg
    56701 aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc
    56761 atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt attggctaca
    56821 cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc tgacgctaat
    56881 ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct ggaaacaaac
    56941 aacggataac gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca aacggttaag
    57001 tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc caaataaatt
    57061 aaaacacggt catgttgatg acgatgcgct gacctttgcc atctttaccc acgacggcag
    57121 aatggtcctt aatgatggcg ataacggaga agatattccc tatagctatc aacgggaagg
    57181 ttttgctgac gggcaactgg tcggtgaaga cgatccttgg cgttttgtct ggatgacctc
    57241 acctgatggc aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg aagacatggc
    57301 gctggcgatt gttgccgggc aattgatccc gtggctggtc gcactgccga ttatgttaat
    57361 catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg cgctggcact
    57421 acgtatgcgt gaccctgact cggaaaaacc actaaacgcg actggcgtac ccagcgaagt
    57481 gcggccactg gttgagtcgc taaatcaact gttcgcccgc acacatgcga tgatggttcg
    57541 agaacgacgc tttacctccg atgcagctca cgaactacgt agcccgttaa cagcgctgaa
    57601 agtgcaaacc gaagttgcgc agctctctga cgatgatccg caggcgcgga aaaaagcact
    57661 gctccaatta cattccggga tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct
    57721 atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc ttgaagatct
    57781 cctacaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa ttgacgtgcg
    57841 actgacactc aatgcccacg gcatcaaacg caccggacaa ccgctattgc taagtttgtt
    57901 ggtgcgaaat ttgctggata acggccccgg tgtgacacca gaggcactgg cgcgaattgg
    57961 cgaacgcttc tatcgcccac ccggacaaac cgctaccggc agtggacttg ggctatcgat
    58021 tgtccagcga atcgccaagc tgcatgacat gaatgttgag tttgggaatg cggaacaagg
    58081 tggatttgag gcgaaggtaa gctggtaact tctctgtcta tgagagccgt taaaacgact
    58141 ctcatagatt ttactaatag caaaatataa accgtcccca aaaaagccac caaccacaaa
    58201 ccagacaaat aacgagcgag aatacatata gccaaaactc gaacatttcc cgcataaaga
    58261 gttttcttaa gataagaata ataagtggcg taagaagaaa aaatgctgca atcattgctt
    58321 gcaatatacg atcttcgcct gaaacggaag gtatactgca aatattgcct cccgactcaa
    58381 gcatccagtc atacttattg aaagcaaaaa aagaatctat taaccaataa gtaagcatta
    58441 tatatggcaa tgcttcaaat attgttttaa ttttctgctt taacatatat cagtacgctc
    58501 ataaaaatcc atttataatt atcaacatga cacgttgata ttttcatctt ttacttattc
    58561 aaagccatat tttcaatccc caaaaaaacc gaaccaaacg tgtaaattat tccggtgcga
    58621 gtaagaatta tgaggaatgg ctatcagtat tgtcattttc agaaaatatt tatcctgcat
    58681 cggtgagtca gagctagatc agacttttgc taaattcgca aaagactttg cacattttgc
    58741 taatttcaca gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgagatta
    58801 aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
    58861 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
    58921 ccgcatcgtt cgcgccgaca gcgactacaa tgtcaaagcg gaagtacaaa actttctctg
    58981 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
    59041 aaaatatatt gatgatgtat tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg
    59101 tacccgcaaa gatccgtcga aaaaatacgg ttccggcagc ctggtacagg acaaaaaata
    59161 tatgctttct ctaacctgga acgcaccaat ggaagccttc accgaaaaag atcagttctt
    59221 ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat aaagcaaacc aattcctcgg
    59281 tatggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
    59341 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
    59401 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
    59461 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
    59521 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
    59581 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
    59641 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
    59701 gaatatccgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
    59761 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
    59821 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
    59881 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
    59941 ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg
    60001 cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc
    60061 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
    60121 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
    60181 gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct
    60241 cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga
    60301 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat
    60361 gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc
    60421 cacgaggaac agttcggtac ggttaaggcc ctgcgcggta caatcagcca gtttgccagg
    60481 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
    60541 ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag
    60601 ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat gccgcacact gacgcgaaga
    60661 gagacgctct ttggtctgcc cggcaaactg cggatcctgc attttaaccg acagaacata
    60721 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
    60781 gtattcacag aactcacgca tcgcgtccaa caggccctga cgcagaccat taacatgggt
    60841 accgccctgc atcgttggga taaggttgac gtagctttcg gtcagcagtt caccgccttc
    60901 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
    60961 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
    61021 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
    61081 gccggggcac agtactgctt tggccttcag cacatgcgtc aggcgtgaaa cagaaaaacg
    61141 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt
    61201 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
    61261 ataaacctga ccatcgcggc gcacgttaac ttctacgcgc ttcgacaagg cgttaactac
    61321 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
    61381 gcctgcatgc agacggcaaa gaatcagttc aaccgccggt actccttctt ccgggtgaat
    61441 atccaccggc atcccgcgcc catcgtcaat cacttctaac gactggtcag catgtaagat
    61501 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
    61561 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
    61621 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
    61681 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
    61741 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
    61801 aagcatagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
    61861 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
    61921 ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt
    61981 ccgtcagcaa ttcaaacggg cgtaccgccg ggtttaccac cactgcgggc agcataaaac
    62041 attgcgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
    62101 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
    62161 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
    62221 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
    62281 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
    62341 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc
    62401 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
    62461 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
    62521 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
    62581 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
    62641 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
    62701 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
    62761 cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
    62821 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
    62881 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
    62941 gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
    63001 tacccctaac agggcttcgt gcttttgtgc aaacaggtga gtatcggtaa tttgtaaaat
    63061 ccttcctctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
    63121 ggtttcacga cgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
    63181 ccagtccgct aaaaactggt taatttgatg cttttcgtcg cgttgatgca actttttatt
    63241 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
    63301 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
    63361 cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg
    63421 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
    63481 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
    63541 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
    63601 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
    63661 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
    63721 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
    63781 ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
    63841 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcaa tcatcccggc
    63901 aaccatctcc agtagccagg gggtttcgct ggtgtcgtac gcggcaatcc gaatctgctc
    63961 aatcagcaca acttcatcac gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
    64021 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa
    64081 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
    64141 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctagc
    64201 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatat
    64261 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
    64321 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
    64381 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
    64441 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
    64501 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
    64561 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
    64621 gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc tgcagttaac tcaatccatt
    64681 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
    64741 gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
    64801 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
    64861 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
    64921 aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
    64981 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggcggcg
    65041 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
    65101 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggagcgcgag
    65161 caaattcgcc aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg
    65221 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
    65281 gatagcaata tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc
    65341 ggaatggtta actcgcaggt gaaacaggca cagtacaact ttgttggtgc cagtgagcaa
    65401 ctggaaagcg cacatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat
    65461 gcttctatca gtagtattaa cgcctacaaa caagccgtag tttccgcgca aagctcatta
    65521 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg
    65581 accaccacgc tgtacaacgc caagcaagag ctggcgaatg cgcgttataa ctacctgatt
    65641 aatcagctga atattaagtc agccctgggt acgttgaacg agcaggattt gctggcactg
    65701 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg
    65761 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcacccgt cgttcagcaa
    65821 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg atgacgacga
    65881 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
    65941 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
    66001 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
    66061 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
    66121 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
    66181 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
    66241 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
    66301 ggtcagtgtc agcaagcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggct
    66361 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
    66421 atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccggccagt
    66481 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
    66541 cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac cactaccgtt
    66601 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc
    66661 ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
    66721 gcccggactg gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
    66781 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
    66841 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
    66901 gcgatgagct gatgaccaaa ttccgcatac caaaacacac ctggagtttt gtgcgtcaat
    66961 catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg
    67021 gcgaacctaa acttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt
    67081 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
    67141 accagtttaa cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt
    67201 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
    67261 ttcagtattt gcaggactgc gcaaccgaag ctgaaatcgc caccgagttc ctctacatcg
    67321 acgagatagg tttaggcgaa aaaggccagt tcacggattt acaggatcag gtgatttcta
    67381 acctgttcaa gctttatccg tgggaattta tgctgcgcga aatgttctcc accaagctgg
    67441 aagatgctgg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaggcac
    67501 ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
    67561 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
    67621 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
    67681 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
    67741 tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc
    67801 gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttgtt gaataagcca
    67861 cgataccgga tggcactcgc catccggtaa ttgttagcct atctgcaccg acagcatact
    67921 taaactgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacac
    67981 acctaacaca tacaacaacg gcagatagtg ctctggcgtt gggttcgata acgcgccacc
    68041 ttcatggtcg agataattca ccagaggatg ctgctctacc ggcccttgcc acgtcagatt
    68101 cgctttcaca tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca
    68161 cttcactgtg cgcaggttat gcaccacgtt accgctggcg accaacatta ttccttcatc
    68221 acgcagcgcc gcgagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
    68281 gatactcaac tgcaccatag ggatatcggc gtcaggatac atcttaatca gcacgcccca
    68341 cgagccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggatcg gcgctaacag
    68401 ctcaaccaga cgctgtgcca gcgcaggcga acccggagca ggataatgcg tatcgtacag
    68461 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
    68521 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    68581 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    68641 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    68701 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    68761 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    68821 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    68881 gtttctcggc gttctcggac aaaaaccctc gaaccgctta ctggcgtttt cgctaggttt
    68941 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    69001 tgaaggaatg tcgccggtgt tgggttatgg aatgtttatc ttcggtctgc ttggctactt
    69061 tggcctggac cgcatgttgc cgcatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    69121 gctgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    69181 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    69241 tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc
    69301 ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact
    69361 agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    69421 ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga
    69481 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    69541 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    69601 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    69661 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    69721 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    69781 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    69841 tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
    69901 atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct
    69961 gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc
    70021 aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag
    70081 cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
    70141 cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt
    70201 gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat
    70261 caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
    70321 aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc
    70381 tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc
    70441 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
    70501 cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg
    70561 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
    70621 ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc
    70681 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
    70741 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
    70801 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
    70861 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
    70921 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
    70981 ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac
    71041 ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta
    71101 cagggaaact acaatgattg acccgaaaaa aattgagca
//
LOCUS       DAJTRM010000005        70711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRM010000005 DAJTRM010000000
VERSION     DAJTRM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.005"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..794
                     /locus_tag="OO322_000379"
     CDS             <1..794
                     /locus_tag="OO322_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5905386.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="OO322_000380"
     CDS             805..1896
                     /locus_tag="OO322_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5905387.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO322_000381"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO322_000381"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5905388.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="OO322_000382"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="OO322_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5905389.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="OO322_000383"
     CDS             4858..5253
                     /locus_tag="OO322_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5905390.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="OO322_000384"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="OO322_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5905391.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="OO322_000385"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="OO322_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5905392.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="OO322_000386"
     CDS             complement(6562..7602)
                     /locus_tag="OO322_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5905393.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="OO322_000387"
     CDS             complement(7675..8310)
                     /locus_tag="OO322_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5905394.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="OO322_000388"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="OO322_000388"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5905395.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="OO322_000389"
     CDS             complement(8936..9379)
                     /locus_tag="OO322_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5905396.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO322_000390"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO322_000390"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5905397.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="OO322_000391"
     CDS             complement(9719..10222)
                     /locus_tag="OO322_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5905398.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="OO322_000392"
     CDS             complement(10216..10740)
                     /locus_tag="OO322_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5905399.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="OO322_000393"
     CDS             10949..11944
                     /locus_tag="OO322_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5905400.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="OO322_000394"
     CDS             11953..12831
                     /locus_tag="OO322_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5905401.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="OO322_000395"
     CDS             12912..13919
                     /locus_tag="OO322_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5905402.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO322_000396"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO322_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5905403.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO322_000397"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO322_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5905404.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO322_000398"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO322_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5905405.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO322_000399"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO322_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5905406.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO322_000400"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO322_000400"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5905407.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO322_000401"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO322_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5905408.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO322_000402"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO322_000402"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5905409.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO322_000403"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO322_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5905410.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO322_000404"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO322_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5905411.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO322_000405"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO322_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5905412.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO322_000406"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO322_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5905413.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="OO322_000407"
     tRNA            complement(27653..27729)
                     /locus_tag="OO322_000407"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="OO322_000408"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="OO322_000408"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5905414.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="OO322_000409"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="OO322_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="OO322_000410"
     tRNA            complement(31513..31599)
                     /locus_tag="OO322_000410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO322_000411"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO322_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5905415.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO322_000412"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO322_000412"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5905416.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO322_000413"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO322_000413"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5905417.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO322_000414"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO322_000414"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5905418.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO322_000415"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO322_000415"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5905419.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="OO322_000416"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="OO322_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5905420.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO322_000417"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO322_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5905421.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="OO322_000418"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="OO322_000418"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5905422.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO322_000419"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO322_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5905423.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="OO322_000420"
     CDS             complement(41211..42176)
                     /locus_tag="OO322_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5905424.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO322_000421"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO322_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5905425.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO322_000422"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO322_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5905426.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="OO322_000423"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="OO322_000423"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5905427.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="OO322_000424"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="OO322_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5905428.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO322_000425"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO322_000425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5905429.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO322_000426"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO322_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5905430.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO322_000427"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO322_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5905431.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO322_000428"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO322_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5905432.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO322_000429"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO322_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5905433.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO322_000430"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO322_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5905434.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO322_000431"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO322_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5905435.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="OO322_000432"
     CDS             49714..50679
                     /locus_tag="OO322_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5905436.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="OO322_000433"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="OO322_000433"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5905437.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="OO322_000434"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="OO322_000434"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5905438.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="OO322_000435"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="OO322_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5905439.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="OO322_000436"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="OO322_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5905440.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="OO322_000437"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="OO322_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5905441.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="OO322_000438"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="OO322_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5905442.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="OO322_000439"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="OO322_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5905443.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="OO322_000440"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="OO322_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5905444.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="OO322_000441"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="OO322_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5905445.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="OO322_000442"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="OO322_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5905446.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58510
                     /gene="yrbL"
                     /locus_tag="OO322_000443"
     CDS             57878..58510
                     /gene="yrbL"
                     /locus_tag="OO322_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5905447.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="OO322_000444"
                     /pseudo
     CDS             complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="OO322_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="OO322_000445"
     CDS             complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="OO322_000445"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5905448.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="OO322_000446"
     CDS             complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="OO322_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5905449.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62546..63475)
                     /locus_tag="OO322_000447"
     CDS             complement(62546..63475)
                     /locus_tag="OO322_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5905450.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64150..68610
                     /gene="gltB"
                     /locus_tag="OO322_000448"
     CDS             64150..68610
                     /gene="gltB"
                     /locus_tag="OO322_000448"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5905451.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68623..70041
                     /gene="gltD"
                     /locus_tag="OO322_000449"
     CDS             68623..70041
                     /gene="gltD"
                     /locus_tag="OO322_000449"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5905452.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70225..70704
                     /locus_tag="OO322_000450"
     CDS             70225..70704
                     /locus_tag="OO322_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5905453.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17315 a        19177 c        17559 g        16660 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgttcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgtattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggcg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagcaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagtgagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgcgct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tcagccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaagtt cgccaggtca gcaccggaga aaccaggagt accacgagca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgatgaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa
    58021 gtactacgcg catcttggtc gccggttaaa agactggagt ggaataccgc gctatcacgg
    58081 taccgttgag acggattgcg gaaccggtta tgtctacgat gtgattgccg attttgacgg
    58141 caagccttcc attacgctga ccgaatttgc tgaacaatgc cgttacgaag aagacattgc
    58201 acaactgcgc cagctactga aacagctgaa gcgttatttg caggataacc gtatcgtgac
    58261 gatgtcgctg aagccgcaga atatcctctg tcaccgcatc agtgagtcag aggttatccc
    58321 ggtcgtctgc gacaacattg gtgaaagcac attgattcca ctggcgacct ggtcgaagtg
    58381 gtgttgtctg cgtaagcaag agcggttatg gaaacgattt atcgcccaac cggcgctggc
    58441 tattgccctg cagaaagatc tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg
    58501 cgaagcttaa tccagctggt gttgttgcat aaacggctca ccgcctaact gatacatctg
    58561 ccgtaaaatc cacgcctgac ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg
    58621 aagtggatta ggtaatacag ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc
    58681 gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc
    58741 ggcgatattc aggtaaaccg tcaggatacg ctttttgctc cagactgttt ctatccccag
    58801 cgttaatcca gcttccagcc cttttcgtac ccagctacgc ccatcccata aaaagagatt
    58861 ttggccgtct gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg
    58921 tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga
    58981 tcttctgcgg caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag
    59041 tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc
    59101 attaccgctg agaagggaac aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa
    59161 acggcgagga caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag
    59221 cggcttttac tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg
    59281 ccgcttctgc aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt
    59341 catcaaccac gatatcatcg acaggacacg gcacatgctc cgcgcccatc tcttccagca
    59401 cttctgcggt atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg
    59461 gcagcatcgc cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt
    59521 gtgccagcgc ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc
    59581 ttaaattctt cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat
    59641 cggcctgggc cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat
    59701 tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat
    59761 ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg
    59821 cttcatgaat ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct
    59881 tcattgtgat catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt
    59941 acgcatcaca catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg
    60001 cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag
    60061 ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt
    60121 ttagtggctt ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc
    60181 atctcttcaa tccattcacc gacgttatct tcccaggcgg gaaggtcagg agactgaatt
    60241 tgctgaccca gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta
    60301 tgtccttcct caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg
    60361 acatagcccg gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct
    60421 acaagttcga gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc
    60481 tcttctgtcg tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc
    60541 gcggttagcg ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg
    60601 tactcttgtt tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg
    60661 cgcggataac gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga
    60721 atatccagca acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg
    60781 gtcatggcga catcaacgct gttacctaat ttttccagca cagaacgcgc gacaatcacg
    60841 ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca
    60901 tcaaacgcat catcgacctc ttctgccacc gacggtgcgt ggatcgtcaa cgtaaaggtt
    60961 gaacctttgc cctgttcact ggtaaccgta atatcgccgc ccatattttt cgccagacga
    61021 cgagaaacgg ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc
    61081 acctggtaat acatggcgaa gattttatcc agctcatcct gcggaatgcc gataccagaa
    61141 tcttccactt caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg
    61201 ccttgctggg tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc
    61261 gtcccgtcgg taatgacctg atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat
    61321 cctttttgtt gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa
    61381 tcaaccggct gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata
    61441 tcgttaaaga tattccccag cgtgacggcc gaaacatgga tagttttgag atatttttcc
    61501 tgctcggcgg tgagttcggt atccagcaga atgcggctca ggccgacgat accgttcagc
    61561 ggtgtacgca attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcgcgt
    61621 tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg
    61681 tgacgtttac ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc
    61741 ccgtccgggt aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt
    61801 tcatcggttt caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg
    61861 tgaaccagtt gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa
    61921 aactctttat cttcgttacg ataaaaaacc aagtcgggtg aagcatcaag gaaggaacgt
    61981 aagaatgagg attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt
    62041 ttcagttggc cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc
    62101 tgatttagct gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc
    62161 atctcctcca gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg
    62221 acgaccaccg atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa
    62281 cgaataacat caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc
    62341 acaatggcaa gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac
    62401 ttcatcatca ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc
    62461 cttcacgaca acctgcccca ataataccca attctgacag ttacgttata aattgtgtga
    62521 gaaatgcgga attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg
    62581 ccgatccctg tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc
    62641 gattttcgca ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt
    62701 ggtagatcac ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtataatcct
    62761 ccagttcaat accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca
    62821 caatatgcag cggatgcagc tttatgccgt ccacgctggt ttcaaccact cgctccagcg
    62881 tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct
    62941 tcagcccgcg ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc
    63001 ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt
    63061 cgtaaccctg gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg
    63121 ggcgggtacc aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag
    63181 aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc
    63241 gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct
    63301 gctgcgcttc atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg
    63361 taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc
    63421 cataacgacg ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct
    63481 gatagaccgc gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga
    63541 cctggatcaa tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa
    63601 tttcatgcac aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct
    63661 tatggatttt aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa
    63721 tgctggtttt ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag
    63781 gaattgactg tttctctaac gactctcctt ttagccttaa agataaaatc tattttaatt
    63841 tcagctattt aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat
    63901 ttgacctgta tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca
    63961 gcctgctcat catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg
    64021 aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac
    64081 gctctttctg tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag
    64141 gttcacgata tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc
    64201 gcccacatag aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc
    64261 cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg
    64321 ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta
    64381 gcaaaaaact acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc
    64441 gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt
    64501 gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt
    64561 gagcaaattt ttgtgaacgc cccggcaggc tggcgtccac gcgatatgga gcgccgtctg
    64621 tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt
    64681 agcctgtcga atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc
    64741 ttttatctgg atcttgcgga cctgcgtctg gaatcggcca tttgcctgtt ccaccagcgc
    64801 ttctccacta acaccgtacc gcgctggccg ctggcgcaac cgttccgcta tctggcgcat
    64861 aacggtgaaa tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa
    64921 ttccagacgc cgctgatccc tgacctgcac gacgccgcgc cattcgtcaa cgaaaccggt
    64981 tctgactcca gttccatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc
    65041 atccgcgcca tgcgcctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg
    65101 gaactgcgtg ccttctttga ctttaactcc atgcatatgg agccgtggga tggtccggcg
    65161 ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc tcgaccgtaa cggtctgcgt
    65221 ccggcacgct acgtcattac caaagacaag ctcatcacct gcgcctctga agtcggtatc
    65281 tgggattacc agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg
    65341 gtgatcgaca ctcgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa
    65401 agccgccacc cttataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa
    65461 gatctgcccg atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac
    65521 cagaaacagt ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa
    65581 aacggtcagg aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt
    65641 cagccgcgca ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca
    65701 atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg
    65761 aacgtctttt gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg
    65821 ctctactccg atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg
    65881 ctggatatca cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctatgc
    65941 gacaaagccg aaaaaatggt acgtagcggc accgtgttgc tggtgctctc cgaccggaat
    66001 atcgctaaag atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt
    66061 ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc
    66121 gatccgcacc acttcgccgt gttgctgggt ttcggcgcga cggctattta tccgtacctt
    66181 gcctatgaaa cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc
    66241 gtgatgctca actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg
    66301 ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctacac
    66361 gatgatgtag tgggcctgtg cttccagggc gcggtcagcc gcattggtgg ggcaagcttt
    66421 gaagacttcc agcaggatct gctgaatctg tcgaaacgtg cctggctggc gcgcaagccg
    66481 attagccagg gcggcctgct gaaatacgtc cacggcggcg aataccacgc ctacaacccg
    66541 gacgtggtgc gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa
    66601 tacgcgaagc tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg
    66661 ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc
    66721 tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg
    66781 gaagcgatga atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg
    66841 cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt
    66901 actccggcgt atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag
    66961 ccgggcgaag gtggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc
    67021 tattcggtgc ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc
    67081 gaagacttag cgcagcttat tttcgacctc aagcaggtta acccgaaagc gatgatctcc
    67141 gttaagctgg tgtccgaacc aggcgttggc accatcgcga ctggcgtggc gaaagcttat
    67201 gcggacttga tcaccatcgc gggctatgac ggcggcaccg gcgcaagccc gctgtcgtca
    67261 gtgaaatatg caggctgccc gtgggaactc ggtctggtgg aaacccaaca ggcgctggtt
    67321 gccaacggct tgcgtcataa aattcgtttg caggttgatg gcggcctgaa aacgggtgtc
    67381 gatatcatca aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg
    67441 gtggcgctcg gctgtaaata tctgcgtatt tgccacctga acaactgcgc cacgggtgta
    67501 gcaactcagg atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgact
    67561 aattactttg agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggagtaacg
    67621 cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc
    67681 aaacagcaga aactggcgct gtcgaagctg ctggagactg ccgagccaca tgcaggtaag
    67741 tcactctact gcactgaaaa caacccgccg tttgataacg gcctactgaa cgcgcagttg
    67801 ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc
    67861 cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc
    67921 gatcaggggc tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt
    67981 ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat
    68041 gtcggtaaag gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc
    68101 cgcagccatg aagcaagcat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt
    68161 ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc
    68221 gtggtagaag gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt
    68281 ctgggtaaaa ccggcgttaa cttcggcgcg ggcatgaccg gcggcttcgc ttacgttctc
    68341 gatgaaagcg gtgatttccg caaacgcgtt aacccggaac tggtcgaagt cttaagcgtc
    68401 gacgatctgg cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat
    68461 accggttctc agcgcggtga agagattctg gcgaactggt caaccttcgc cacgaaattc
    68521 gcgctggtta aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc
    68581 gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat
    68641 caatttatcg acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgtaaaatt
    68701 gagtttgttg aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc
    68761 tgtctgtcgt gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatccca
    68821 aactggctga agctcgccaa cgaggggcgt atttttgaag cggcagaact gtcgcatcag
    68881 accaacacac tgccggaagt atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt
    68941 tcctgcaccc tgaacgatga gttcggtgcg gtgaccatcg gcaacattga gcgttatatc
    69001 aatgataaag cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt
    69061 aaaaaagtgg cgattatcgg tgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg
    69121 cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc
    69181 ttcggtattc cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc
    69241 accggcatgg gtattgaatt caaactcaat accgaagtgg gccgcgacgt acagctggac
    69301 gatctgctga gtgattacga tgccgtgttc cttggcgttg ggacttatca gtcaatgcgc
    69361 ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc
    69421 aacaccaaac agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa
    69481 ggcaaacgcg tggtagtact tggcggtggc gacactgcga tggactgcgt gcgcacgtct
    69541 gtacgccagg gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg
    69601 ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc
    69661 cagccactgg ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt
    69721 accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc
    69781 gaacatatcg ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg
    69841 gaatggctgg caaaacacag cgtcgagctg gattcacaag gccgcatcat cgctccggaa
    69901 ggcaacgaca acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc
    69961 cgtggttccg atctggtcgt taccgccatc gccgaaggtc gcaaagcggc agacggcatt
    70021 atgaactggc tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg
    70081 ctgaaattct cctttctttc tggaaatcgc ctcgcataat ctgaagaaaa ccaatgtaat
    70141 acgctcatct ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg
    70201 cagcgccata taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac
    70261 cagcaaatcc acaacggcat tattcatctg gtcgatagcg cccgaacgga aacggtacgt
    70321 agcgttaacg cgctgatgac cgcgacgtac tgggaaattg gccgacggat tgtcgaattt
    70381 gaacaaggtg gtgaggccag agcagcttat ggtgcacagt tgattgatcg gctatcgaag
    70441 gatttaagtc gacggtataa acgtggggtc tctgcaaaaa acttacgcca aatgaggctt
    70501 ttttacctct tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca
    70561 ttgggaattc cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg
    70621 tctgccaaat tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt
    70681 cggtttgtat attcatgcaa ataaacgaag g
//
LOCUS       DAJTRM010000006        67673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRM010000006 DAJTRM010000000
VERSION     DAJTRM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.006"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..229
                     /locus_tag="OO322_000451"
     CDS             <1..229
                     /locus_tag="OO322_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5905454.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="OO322_000452"
                     /pseudo
     CDS             624..1263
                     /locus_tag="OO322_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="OO322_000453"
     CDS             1366..1689
                     /locus_tag="OO322_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5905455.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO322_000454"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO322_000454"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5905456.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO322_000455"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO322_000455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5905457.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO322_000456"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO322_000456"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5905458.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO322_000457"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO322_000457"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5905459.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO322_000458"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO322_000458"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5905460.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO322_000459"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO322_000459"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5905461.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO322_000460"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO322_000460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5905462.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO322_000461"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO322_000461"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5905463.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO322_000462"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO322_000462"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5905464.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO322_000463"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO322_000463"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5905465.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO322_000464"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO322_000464"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5905466.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO322_000465"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO322_000465"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5905467.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO322_000466"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO322_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5905468.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO322_000467"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO322_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5905469.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="OO322_000468"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="OO322_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5905470.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="OO322_000469"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="OO322_000469"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5905471.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="OO322_000470"
     CDS             complement(21227..23059)
                     /locus_tag="OO322_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5905472.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO322_000471"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO322_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5905473.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="OO322_000472"
     CDS             complement(23807..24301)
                     /locus_tag="OO322_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5905474.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /locus_tag="OO322_000473"
     CDS             complement(24384..24839)
                     /locus_tag="OO322_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5905475.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="OO322_000474"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="OO322_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5905476.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="OO322_000475"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="OO322_000475"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5905477.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="OO322_000476"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="OO322_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5905478.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO322_000477"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO322_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5905479.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO322_000478"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO322_000478"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5905480.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO322_000479"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO322_000479"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5905481.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="OO322_000480"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="OO322_000480"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5905482.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="OO322_000481"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="OO322_000481"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5905483.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="OO322_000482"
     CDS             33415..34308
                     /locus_tag="OO322_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5905484.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO322_000483"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO322_000483"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5905485.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO322_000484"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO322_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5905486.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="OO322_000485"
     CDS             complement(36224..36940)
                     /locus_tag="OO322_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5905487.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO322_000486"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO322_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5905488.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO322_000487"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO322_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5905489.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO322_000488"
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO322_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5905490.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO322_000489"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO322_000489"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5905491.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO322_000490"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO322_000490"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5905492.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO322_000491"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO322_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5905493.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO322_000492"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO322_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5905494.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO322_000493"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO322_000493"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5905495.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO322_000494"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO322_000494"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5905496.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="OO322_000495"
     CDS             complement(45789..46448)
                     /locus_tag="OO322_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5905497.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO322_000496"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO322_000496"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5905498.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="OO322_000497"
     CDS             complement(47343..48356)
                     /locus_tag="OO322_000497"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5905499.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO322_000498"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO322_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5905500.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="OO322_000499"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="OO322_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5905501.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="OO322_000500"
     CDS             complement(50649..51827)
                     /locus_tag="OO322_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5905502.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO322_000501"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO322_000501"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5905503.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="OO322_000502"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="OO322_000502"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5905504.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="OO322_000503"
     CDS             complement(55609..55887)
                     /locus_tag="OO322_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5905505.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO322_000504"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO322_000504"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5905506.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="OO322_000505"
     CDS             complement(56469..57278)
                     /locus_tag="OO322_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5905507.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO322_000506"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO322_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5905508.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO322_000507"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO322_000507"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5905509.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO322_000508"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO322_000508"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5905510.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="OO322_000509"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="OO322_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5905511.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="OO322_000510"
     CDS             complement(60979..61869)
                     /locus_tag="OO322_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5905512.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="OO322_000511"
     CDS             complement(61856..62380)
                     /locus_tag="OO322_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5905513.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="OO322_000512"
     CDS             complement(62377..62847)
                     /locus_tag="OO322_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5905514.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="OO322_000513"
     CDS             complement(62844..63392)
                     /locus_tag="OO322_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5905515.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="OO322_000514"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="OO322_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..64861)
                     /locus_tag="OO322_000515"
     CDS             complement(64139..64861)
                     /locus_tag="OO322_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5905516.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO322_000516"
                     /pseudo
     CDS             complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO322_000516"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66024..66779)
                     /locus_tag="OO322_000517"
     CDS             complement(66024..66779)
                     /locus_tag="OO322_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5905517.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66861..67424)
                     /locus_tag="OO322_000518"
     CDS             complement(66861..67424)
                     /locus_tag="OO322_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5905518.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16925 a        18281 c        16785 g        15682 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaatga
      601 cgccttttat tgataattta catatgaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgtttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aagcctaatt atttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaagaa acatacaacg catgatacat atccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32521 aaccgttaag cctctttgag gctgctgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact aacagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgagaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgttcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca atatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
    66121 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
    66181 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
    66241 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
    66301 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
    66361 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
    66421 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
    66481 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
    66541 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
    66601 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
    66661 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
    66721 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
    66781 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
    66841 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
    66901 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
    66961 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
    67021 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
    67081 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
    67141 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
    67201 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
    67261 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
    67321 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
    67381 atcaccattg ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact
    67441 ataaataatt aaaaatataa acattgcaat acattgattc agtcaatagc caatgtttat
    67501 acccagccat tgaatacggc atgcgaccca aaaagaacag gtcgtagaaa attgtattca
    67561 ttggtgtggg gccgcatagt gatgtttcca cacccctgat ccaatttaat tttactaaag
    67621 agtaaatgta gatttaatta atatattgac agaagaaagg ttatcttaag gac
//
LOCUS       DAJTRM010000007        66657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRM010000007 DAJTRM010000000
VERSION     DAJTRM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.007"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..742)
                     /gene="cra"
                     /locus_tag="OO322_000519"
     CDS             complement(<1..742)
                     /gene="cra"
                     /locus_tag="OO322_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5905519.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSF"
     gene            complement(824..910)
                     /locus_tag="OO322_000520"
     CDS             complement(824..910)
                     /locus_tag="OO322_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5905520.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(922..1413)
                     /gene="ilvN"
                     /locus_tag="OO322_000521"
     CDS             complement(922..1413)
                     /gene="ilvN"
                     /locus_tag="OO322_000521"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5905521.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1416..3140)
                     /gene="ilvI"
                     /locus_tag="OO322_000522"
     CDS             complement(1416..3140)
                     /gene="ilvI"
                     /locus_tag="OO322_000522"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5905522.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3259..3303
                     /locus_tag="OO322_000523"
     CDS             3259..3303
                     /locus_tag="OO322_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905523.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3458..4402)
                     /gene="leuO"
                     /locus_tag="OO322_000524"
     CDS             complement(3458..4402)
                     /gene="leuO"
                     /locus_tag="OO322_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5905524.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5061..5147
                     /gene="leuL"
                     /locus_tag="OO322_000525"
     CDS             5061..5147
                     /gene="leuL"
                     /locus_tag="OO322_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5905525.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5240..6811
                     /gene="leuA"
                     /locus_tag="OO322_000526"
     CDS             5240..6811
                     /gene="leuA"
                     /locus_tag="OO322_000526"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5905526.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            6811..7902
                     /gene="leuB"
                     /locus_tag="OO322_000527"
     CDS             6811..7902
                     /gene="leuB"
                     /locus_tag="OO322_000527"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5905527.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            7905..9305
                     /gene="leuC"
                     /locus_tag="OO322_000528"
     CDS             7905..9305
                     /gene="leuC"
                     /locus_tag="OO322_000528"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5905528.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9316..9921
                     /gene="leuD"
                     /locus_tag="OO322_000529"
     CDS             9316..9921
                     /gene="leuD"
                     /locus_tag="OO322_000529"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5905529.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(9970..11148)
                     /gene="setA"
                     /locus_tag="OO322_000530"
     CDS             complement(9970..11148)
                     /gene="setA"
                     /locus_tag="OO322_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5905530.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11250..11381)
                     /gene="sgrT"
                     /locus_tag="OO322_000531"
     CDS             complement(11250..11381)
                     /gene="sgrT"
                     /locus_tag="OO322_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5905531.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11470..13125
                     /gene="sgrR"
                     /locus_tag="OO322_000532"
     CDS             11470..13125
                     /gene="sgrR"
                     /locus_tag="OO322_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5905532.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13159..13258
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13289..14272
                     /gene="thiB"
                     /locus_tag="OO322_000533"
     CDS             13289..14272
                     /gene="thiB"
                     /locus_tag="OO322_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5905533.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14248..15858
                     /gene="thiP"
                     /locus_tag="OO322_000534"
     CDS             14248..15858
                     /gene="thiP"
                     /locus_tag="OO322_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5905534.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15842..16540
                     /gene="thiQ"
                     /locus_tag="OO322_000535"
     CDS             15842..16540
                     /gene="thiQ"
                     /locus_tag="OO322_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5905535.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16654..17418)
                     /locus_tag="OO322_000536"
     CDS             complement(16654..17418)
                     /locus_tag="OO322_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5905536.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17504..18382)
                     /gene="araC"
                     /locus_tag="OO322_000537"
     CDS             complement(17504..18382)
                     /gene="araC"
                     /locus_tag="OO322_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5905537.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18721..20421
                     /gene="araB"
                     /locus_tag="OO322_000538"
     CDS             18721..20421
                     /gene="araB"
                     /locus_tag="OO322_000538"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5905538.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20432..21934
                     /gene="araA"
                     /locus_tag="OO322_000539"
     CDS             20432..21934
                     /gene="araA"
                     /locus_tag="OO322_000539"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5905539.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22134..22829
                     /gene="araD"
                     /locus_tag="OO322_000540"
     CDS             22134..22829
                     /gene="araD"
                     /locus_tag="OO322_000540"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5905540.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            22904..25255
                     /gene="polB"
                     /locus_tag="OO322_000541"
     CDS             22904..25255
                     /gene="polB"
                     /locus_tag="OO322_000541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5905541.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25420..28326
                     /gene="rapA"
                     /locus_tag="OO322_000542"
     CDS             25420..28326
                     /gene="rapA"
                     /locus_tag="OO322_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5905542.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28338..28997
                     /gene="rluA"
                     /locus_tag="OO322_000543"
     CDS             28338..28997
                     /gene="rluA"
                     /locus_tag="OO322_000543"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5905543.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29114..29929)
                     /gene="djlA"
                     /locus_tag="OO322_000544"
     CDS             complement(29114..29929)
                     /gene="djlA"
                     /locus_tag="OO322_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5905544.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30184..32538
                     /gene="lptD"
                     /locus_tag="OO322_000545"
     CDS             30184..32538
                     /gene="lptD"
                     /locus_tag="OO322_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5905545.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32591..33877
                     /gene="surA"
                     /locus_tag="OO322_000546"
     CDS             32591..33877
                     /gene="surA"
                     /locus_tag="OO322_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5905546.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            33877..34866
                     /gene="pdxA"
                     /locus_tag="OO322_000547"
     CDS             33877..34866
                     /gene="pdxA"
                     /locus_tag="OO322_000547"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5905547.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34863..35684
                     /gene="rsmA"
                     /locus_tag="OO322_000548"
     CDS             34863..35684
                     /gene="rsmA"
                     /locus_tag="OO322_000548"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5905548.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35687..36064
                     /gene="apaG"
                     /locus_tag="OO322_000549"
     CDS             35687..36064
                     /gene="apaG"
                     /locus_tag="OO322_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5905549.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36071..36913
                     /gene="apaH"
                     /locus_tag="OO322_000550"
     CDS             36071..36913
                     /gene="apaH"
                     /locus_tag="OO322_000550"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5905550.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(36991..37470)
                     /gene="folA"
                     /locus_tag="OO322_000551"
     CDS             complement(36991..37470)
                     /gene="folA"
                     /locus_tag="OO322_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5905551.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37661..39523)
                     /gene="kefC"
                     /locus_tag="OO322_000552"
     CDS             complement(37661..39523)
                     /gene="kefC"
                     /locus_tag="OO322_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5905552.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39516..40046)
                     /gene="kefF"
                     /locus_tag="OO322_000553"
     CDS             complement(39516..40046)
                     /gene="kefF"
                     /locus_tag="OO322_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5905553.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40094..41488)
                     /locus_tag="OO322_000554"
     CDS             complement(40094..41488)
                     /locus_tag="OO322_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5905554.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41547..41834)
                     /gene="fixX"
                     /locus_tag="OO322_000555"
     CDS             complement(41547..41834)
                     /gene="fixX"
                     /locus_tag="OO322_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5905555.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41831..43117)
                     /locus_tag="OO322_000556"
     CDS             complement(41831..43117)
                     /locus_tag="OO322_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5905556.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43168..44109)
                     /locus_tag="OO322_000557"
                     /pseudo
     CDS             complement(43168..44109)
                     /locus_tag="OO322_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44124..44894)
                     /locus_tag="OO322_000558"
     CDS             complement(44124..44894)
                     /locus_tag="OO322_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5905557.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45250..46764
                     /gene="caiT"
                     /locus_tag="OO322_000559"
     CDS             45250..46764
                     /gene="caiT"
                     /locus_tag="OO322_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5905558.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            46795..47937
                     /gene="caiA"
                     /locus_tag="OO322_000560"
     CDS             46795..47937
                     /gene="caiA"
                     /locus_tag="OO322_000560"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5905559.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48066..49283
                     /gene="caiB"
                     /locus_tag="OO322_000561"
     CDS             48066..49283
                     /gene="caiB"
                     /locus_tag="OO322_000561"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5905560.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49357..50910
                     /gene="caiC"
                     /locus_tag="OO322_000562"
     CDS             49357..50910
                     /gene="caiC"
                     /locus_tag="OO322_000562"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5905561.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51019..51804
                     /gene="caiD"
                     /locus_tag="OO322_000563"
     CDS             51019..51804
                     /gene="caiD"
                     /locus_tag="OO322_000563"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5905562.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            51810..52400
                     /gene="caiE"
                     /locus_tag="OO322_000564"
     CDS             51810..52400
                     /gene="caiE"
                     /locus_tag="OO322_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5905563.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52486..52881)
                     /gene="caiF"
                     /locus_tag="OO322_000565"
     CDS             complement(52486..52881)
                     /gene="caiF"
                     /locus_tag="OO322_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5905564.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53143..56364)
                     /gene="carB"
                     /locus_tag="OO322_000566"
     CDS             complement(53143..56364)
                     /gene="carB"
                     /locus_tag="OO322_000566"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5905565.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56382..57530)
                     /gene="carA"
                     /locus_tag="OO322_000567"
     CDS             complement(56382..57530)
                     /gene="carA"
                     /locus_tag="OO322_000567"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5905566.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(57986..58807)
                     /gene="dapB"
                     /locus_tag="OO322_000568"
     CDS             complement(57986..58807)
                     /gene="dapB"
                     /locus_tag="OO322_000568"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5905567.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(58974..59888)
                     /gene="rihC"
                     /locus_tag="OO322_000569"
     CDS             complement(58974..59888)
                     /gene="rihC"
                     /locus_tag="OO322_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5905568.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(59954..60904)
                     /gene="ispH"
                     /locus_tag="OO322_000570"
                     /gene_synonym="lytB"
     CDS             complement(59954..60904)
                     /gene="ispH"
                     /locus_tag="OO322_000570"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5905569.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(60906..61355)
                     /gene="fkpB"
                     /locus_tag="OO322_000571"
     CDS             complement(60906..61355)
                     /gene="fkpB"
                     /locus_tag="OO322_000571"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5905570.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61442..61936)
                     /gene="lspA"
                     /locus_tag="OO322_000572"
     CDS             complement(61442..61936)
                     /gene="lspA"
                     /locus_tag="OO322_000572"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5905571.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(61936..64752)
                     /gene="ileS"
                     /locus_tag="OO322_000573"
     CDS             complement(61936..64752)
                     /gene="ileS"
                     /locus_tag="OO322_000573"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5905572.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(64795..65736)
                     /gene="ribF"
                     /locus_tag="OO322_000574"
     CDS             complement(64795..65736)
                     /gene="ribF"
                     /locus_tag="OO322_000574"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5905573.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(65744..65962)
                     /locus_tag="OO322_000575"
     CDS             complement(65744..65962)
                     /locus_tag="OO322_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5905574.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66065..66328
                     /gene="rpsT"
                     /locus_tag="OO322_000576"
     CDS             66065..66328
                     /gene="rpsT"
                     /locus_tag="OO322_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5905575.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16176 a        18146 c        17153 g        15182 t
ORIGIN      
        1 caaacgacgt tgtgaacagc gcctgcggca tcggatgcgt ttccagccat ttttcgaata
       61 actgggcagc cgcctcccgc tcatagctgt tggcatacag gaaatgcact tcgcgcggat
      121 catctttcca agcagtacgg aaaccttgtt cacgcaggaa gctgacagaa agctccggca
      181 gcgcgccaag ataaagcacc gtctcggcgg gaaacttacg taactcttcc gccagcattt
      241 cggcatcatc ctgatcggca ccaaccacgc tggtgaaatg ttcacgatcg agggcgcggt
      301 ccagcgcgac aatcgggaac gggtcgttag cccagcgttg ataaaaagga tgctcaggtg
      361 gcaacgacgt cgaaacaata atggcatcaa cctgacgctg taaaaggtgc tcaatgcagc
      421 gcatttcgtt gtctggctga tcttctgagc aggcaatcag cagttgataa ccccgttgcc
      481 gcgcctggcg ttcaagatag ttagcgatgc gggtatagct ggtgttctcc agatcgggga
      541 tcacaagacc aatagaacgt gtgcgtccag cacgaagccc agctgccacg gcgttcgggt
      601 ggtaattgtg ctcacgcacc acagccatga ctttttcaac ggttttgtcg ctcacacggt
      661 attgcttcgc tttgccgtta ataacatagc ttgcagtggt ccgcgacact cccgccagcc
      721 gagcgatttc atccagtttc acaattgccc cttgcgtaaa atgtaaaaac catagccatt
      781 gtactggtat gggttaaatt cattaacatc taagcgcaga aaattaactg cggcaactgc
      841 ttttattcca ggttcccact gatttcgcaa aaaaagccca acgtgacatg ttgggctgca
      901 aattgcgcac tgagatcatt ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac
      961 accagagcga gcaacttcca caattttcgc cacatcgcga atcgatgcta aaaatgcatc
     1021 aagcttatcg ctggtgcctg ctaattgaac ggtataaagc gagggggtga catcgataat
     1081 ttgcccacgg aatatttccg tattacgttt cacttcgtca cgcccgtaac cgctggcctg
     1141 aattttcacc agcatgattt cccgctcaac atgcgcgccc tgccccaact cactcacgcg
     1201 caagacatcg accagcttgt gtaattgctt ttcgatctgc tcaagtactt tttcatcgcc
     1261 cacggtctgg atggtcatac gcgataatgt cggatcgtcg gttggcgcaa cggtcaggct
     1321 ttcaatgttg tagcctcgct gggaaaaaag gccaatcacg cgggataacg cgcctgattc
     1381 attttcgagt aagactgata atatccggcg cataatcagg ttctctccgt tttgcttaac
     1441 cacatttcat ccattccgcc cccgcgaatc tgcatcgggt agacgtgctc actgccatcg
     1501 acggtaacat caacaaacac caggcgatta ttgcgcacct gttccagcgc ctcgctaagt
     1561 ttgctttcca gctcatgcgg atgagagatc tggatcccga cgtgaccata ggcttccgcc
     1621 agacggacga aatcgggtag tgattgcata taagattgtg aatgacggcc ggaatagatc
     1681 atgtcctgcc actgtttcac catccccaga tagcggttat tgagattcac caccagtacg
     1741 ggcaactcgt attgcaacgc ggtagacagt tcctggatgt tcatctgaat actgccgtcg
     1801 ccagtgacgc aaaccacggt ttcttctggc aacgccattt tgacgcccag tgccgcaggt
     1861 aaaccaaaac ccatcgtgcc gagaccgccg gaattgatcc agcgacgcgg tttgtcgaat
     1921 ggataataaa gcgcagcaaa catctggtgc tgcccgacat cggacgtcac gtaagcgtcc
     1981 ccattagtca accgccaaag agtctcgatc accgcctgcg gtttaatctt ttcactgtga
     2041 gtgtcatatt tcaggcactg aagagcgcgc cactgttcaa tctgctgcca ccagtcgcgg
     2101 atctcatcca gcggttgatg ggcggattct tgcgacagga gttcaagcat ttgttcgagg
     2161 acctggcgag catcccccac aatcgggata tccgcagtca cggttttaga aatggaagta
     2221 ggatcaatat cgatatgcag aacagtggca tttgggcagt actttgccag attgttcgtc
     2281 gttcggtcat caaatcgtac cccgacggcg aaaatcacat ccgcgttatg catcgtcata
     2341 ttggcttcgt aggtaccgtg cattcccagc atgcccagtg cctgacgatg cgttgccgga
     2401 aacgccccca ttcccatcaa tgaggaaaca acgggcagat tcaacgcctc caccgtttct
     2461 ttcaactgct gatggcagcc cgccatgatt gccccaccgc ctacgtagac aaccggtttt
     2521 tttgccgcta ccagcgtttg cagagcacgc ttaatttgcc ctttatgtcc ggtagtagtg
     2581 ggattgtaag aacgcatact gaccgactca gggcagacat agggtaattt gttcgccgga
     2641 ttaagaatat ctttcggtaa atcaacgacc actggtcctg ggcgaccact tgccgccagc
     2701 cagaaagcct ttttcagcac ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt
     2761 ttaaccaccg gtcgcgaaat ccccaccatg tcgcactcct gaaaggcatc gtaacctatc
     2821 aacgaggtcg ctacctgccc ggaaaggaca actaatggaa tggaatccat ataagcggtg
     2881 gcgatgccgg taatcgcatt ggtcgccctt ggacccgacg ttaccagcac gacgccgact
     2941 tccccggtcg cacgcgccag gccatcggcc atatgcaccg ccgcctgctc atgacgaact
     3001 aatacatgat caataccacc cacggtatgc aatgcatcgt aaatatcaag gactgcgcct
     3061 ccgggataac cgaatacttg tttaacgccc tgatcgataa gcgatcggac gaccatctcg
     3121 gctccagaca acatctccat ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac
     3181 attaaccgtc cgatcttgct taagcaattg gcagccagag acataaacgc agggtaataa
     3241 gaataaacag gaggaaaggt gaaaagaaac gataaaatcc tccattacga aggattaaat
     3301 tgataataaa tcggctgaat cccacaactt acagcaattt ctctattttt ttaattaatc
     3361 ctaaaataga gagtccacac attcagaata aaattctaca ttcaataaaa aatgcagaat
     3421 aaaccagaca ttcatgtctg gcctattctg cattcagtta gcgtttgcaa attgagacta
     3481 attgttcttc catccactga tggcctttat cgcgcccggc agcttcatgc caagagagat
     3541 aacaggttct gctgttttgt tttaacggca gcggtaatac ctgtaattct aaggattcag
     3601 cgaactcttc agccagccaa cgcggcgcaa tagcgaccaa atgcgtttgc gacaccacgc
     3661 taagtacgct catcattgcc atgccctgat acgcgatact ggcttgctta tctaccgtgt
     3721 cataccaagg ttgactaaat gacgcgaaac gatcgagcga aaccgccgca tgttgttcgt
     3781 tataaacatc atgtttcagt aacgggcctt taattgttgg atgatttttg ctggctacca
     3841 gcaccatttc atctttaaat aatggcacgc tggtaaattc aggacgatgg aagtcttcat
     3901 aactaatcac aaactccgtt tcctgataac gcagctgatg ttcagtgttc tgatttaatg
     3961 aagacttgaa cataacatgt atatttggcg caatctgctc aatgtgatta taaatctgcg
     4021 aggtcagaat gctgtctaac gggctgcaaa cacaaagatg aaatacacgt tcactgctcg
     4081 cgggttcaaa acctgaacca ggcaattcat tttgtactag ttgcaatgcc tggcgaactg
     4141 aaccaaaaag ttgaaatgcg cgagcagtcg gttgaatacc acggccataa cgaacaaaaa
     4201 gctcgtcatt aaacatcacc ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca
     4261 tacccagaac atgagcggca cgggtaatgt tttgctcctg catcacggca tcgaaaacgg
     4321 ttaataagtt gagatcgacc atgcgtagct gtggcttgct taactccgcc gtctctgaat
     4381 gatctgtttg tacctctggc atacttaact ccactgtcac acttaactcc ctttccctta
     4441 ttggaatgca gagaaataat cctgaagaat atgatttatc atgcatataa aataagaaaa
     4501 agcaaaatga gtaaaattcg gaaaacataa agatacgggt tctgataaaa acagaatcca
     4561 ttgccgtgga atgagtcatt taccacagca taataatcca taatgttttg ttgcgaaaac
     4621 aatctaatca taactaccgc gaatactcaa tcatctacaa aatggattaa atgtgcttat
     4681 ttaataatta atttatatat ttaatgcatt aattcttaac attaattgtt caataatatt
     4741 caccaaatca atatcaaaaa aaatcgcaaa acatataatt caatacaaat catcaggata
     4801 ggttttgcaa cgcgtgcatt ttgtcccctt tttcctcgtt gattagatgc aaaaatttat
     4861 gctgaaatat gtcaaccgat gaaaagcgtc ggtagttaag cagaaattaa tatcgcttac
     4921 tttaaccacc gtagcacaat tagctaattt tacggatgca gaactcacgc tggcgggacg
     4981 tttttattgc gtcagggttg acatccgttt ttgtatccag taactctaaa agcatatcgc
     5041 attcatctgg agctgattta atgactcaca tcgttcgctt tatcggtcta ctactactaa
     5101 acgcatcttc tttgcgcggt agacgagtga gcggcatcca gcattaagcc agcacgcagt
     5161 caaacaaaaa acccgcgcca ttgcgcgggt ttttttatgc ccgaagcgag gcgctctaaa
     5221 agagacaagg acccaaacca tgagccagca agtcattatt ttcgatacca cattgcgcga
     5281 cggtgaacag gcgttacagg caagcttgag tgtgaaagaa aaactgcaaa ttgcgctggc
     5341 ccttgagcgt atgggtgttg acgtgatgga agtcggtttc cccgtctctt cgccgggcga
     5401 ttttgaatcg gtgcaaacca tcgcccgcca ggttaaaaac agccgcgtat gtgcgttagc
     5461 tcgctgcgtg gaaaaagata tcgacgtggc ggccgaatcc ctgaaagtcg ccgaagcctt
     5521 ccgtattcat acctttattg ctacctcccc aatgcacatc gccaccaagc tgcgcagcac
     5581 cctagacgag gtgatcgaac gcgctatcta tatggtgaaa cgcgcccgta attacaccga
     5641 tgatgttgaa ttttcttgcg aagatgccgg gcgtacaccc attgccgatc tggcgcgagt
     5701 ggtcgaagcg gcgattaatg ccggtgccac caccatcaac attccggaca ccgtgggcta
     5761 caccatgccg tttgagttcg ccggaatcat cagcggcctg tatgaacgcg tacctaacat
     5821 cgacaaagcc attatttccg tacataccca cgacgattta ggcctggcgg tcggcaactc
     5881 actggcggcg gtacatgccg gtgcacgcca ggtggaaggt gcaatgaacg ggatcggcga
     5941 gcgtgccggt aactgttcgc tggaagaagt catcatggcg attaaagttc gtaaggatat
     6001 tctcaacgtc cacaccgcca ttaatcacca agagatatgg cgcaccagcc agttagttag
     6061 ccagatttgt aatatgccga tcccggcaaa caaagccatt gttggcagcg gcgcattcgc
     6121 tcactcctcc ggtatccacc aggatggcgt gctgaaaaac cgcgaaaact acgaaatcat
     6181 gacaccagaa tctattggtc tgaaccaaat ccagctgaat ctgacctctc gttcggggcg
     6241 tgcggcggtg aaacatcgca tggatgagat ggggtataaa gaaagtgaat ataatttaga
     6301 caatttgtac gatgctttcc tgaagctggc ggacaaaaaa ggtcaggtgt ttgattacga
     6361 tctggaggcg ctggccttca tcggtaagca gcaagaagag ccggagcatt tccgtctgga
     6421 ttacttcagc gtgcagtctg gctctaacga tatcgccacc gccgccgtca aactggcctg
     6481 tggcgaagaa gtcaaagcag aagccgccaa cggtaacggt ccggtcgatg ccgtctatca
     6541 ggcaattaac cgcatcactg actataacgt cgaactggtg aaatacagcc tgaccgccaa
     6601 aggtcacggt aaagatgcgc tgggtcaggt ggatattgtc accaactaca acggtcgccg
     6661 cttccacggc gtcggcctgg ccaccgatat tgtcgagtcc tccgccaaag ccatggtgca
     6721 cgtactgaac aatatctggc gtgccgcaga agtcgaaaaa gagttgcaac gcaaaactca
     6781 acacaacgaa aacaacaagg aaaccgtgtg atgtcgaaga attaccatat tgccgtattg
     6841 ccgggagacg gtattggtcc ggaagtgatg acccaggcgc tgaaagtgct gaatgccgtg
     6901 cgcaaccgct ttgcgatgcg catcaccacc agccattacg atgtaggcgg cgcagccatt
     6961 gataaccacg ggcaaccact gccgcctgcg acggttgaag gttgtgagca agccgatgcc
     7021 gtgctgtttg gctcggtagg cggcccgaag tgggaacatt taccaccaga ccagcaacca
     7081 gaacgcggcg cgctgctgcc gctgcgtaag cacttcaaat tattcagcaa cctgcgcccg
     7141 gcaaaactgt atcaggggct ggaagcattc tgtccgctgc gtgcagacat tgccgctaac
     7201 ggcttcgaca tccagtgcgt gcgcgaactg accggcggca tctatttcgg tcagccaaaa
     7261 ggccgcgaag gtagcggaca atatgaaaaa gcgtttgata ccgaggtgta tcaccgtttt
     7321 gagatcgagc gtattgcccg catcgcgttt gaatctgctc gtaaacgtcg ccacaaagtc
     7381 acctcaatcg acaaagctaa cgtgctgcaa tcctctattt tatggcgtga aatcgtcaac
     7441 gagatcgcca cggaataccc ggatgtcgaa ctggcgcata tgtacatcga caacgccacc
     7501 atgcagctga ttaaagatcc atcacagttt gacgttctgc tgtgctccaa cctgtttggc
     7561 gacattctgt ctgacgagtg cgcaatgatc actggctcga tgggcatgtt gccttccgcc
     7621 agcctgaacg agcaaggttt tggactgtat gaaccggcgg gcggctcggc accagatatc
     7681 gcaggcaaaa acatcgccaa cccgattgca caaatcctgt cgctggcact gctgctgcgt
     7741 tacagcctgg atgccgatga tgcggcttcc gccattgaac gcgccattaa ccgtgcatta
     7801 gaagaaggca ttcgcaccgg ggatttagcc cgtggcgctg ccgccgttag taccgatgaa
     7861 atgggcgata tcattgcccg ctatgtggca gaaggggtgt aatcatggct aaaacgttat
     7921 acgaaaaatt attcgacgct cacgtagtgt acgaagccga aaacgaaacc ccgctgttat
     7981 atatcgaccg ccacctggtg catgaagtga cctcaccgca ggcgttcgat ggtctgcgcg
     8041 cccacggtcg cccggtacgt cagccgggca aaaccttcgc cactatggat cacaacgtct
     8101 ctacccagac caaagacatt aatgcctgtg gtgaaatggc gcgcatccag atgcaggagc
     8161 tgattaaaaa ctgcaaagaa tttggcgtcg agctgtatga cctgaatcac ccgtatcagg
     8221 ggatcgtcca cgtaatgggg ccggaacagg gcgtcacctt gccggggatg accattgtct
     8281 gcggcgactc gcataccgcc acccacggcg cgtttggcgc actggccttt ggtatcggca
     8341 cttccgaagt tgaacacgta ctggcaacac aaaccctgaa acagggccgt gcgaagacca
     8401 tgaaaattga agtccagggc aaagccgcgc cgggcattac cgcaaaagat atcgtgctgg
     8461 caattatcgg taaaactggc agcgcaggcg gcaccgggca tgtggtggag ttttgcggcg
     8521 aagcaattcg tgatttaagc atggaaggtc gtatgaccct gtgcaatatg gcgatcgaaa
     8581 tgggcgcaaa agccgctctg gttgctcctg acgaaaccac ctttaactat gtcaaaggcc
     8641 gtctgcatgc gccgaaaggc aaagatttcg acgacgccgt tgcctactgg aaaaccctgc
     8701 aaaccgacga aggcgcaact ttcgataccg ttgtcactct gcaagcagaa gaaatttcac
     8761 cgcaggtcac ctggggcacc aatcccggcc aggtgatttc tgtgaacgac aatattcccg
     8821 atccggcttc gtttgccgat ccggttgaac gtgcgtcggc agaaaaagcg ctggcctata
     8881 tggggctgaa accgggtatt ccgctgaccg aagtggctat cgacaaagtg tttatcggtt
     8941 cctgtaccaa ctctcgcatt gaagatttac gcgcggcagc ggagatcgcc aaagggcgaa
     9001 aagtcgcgcc aggcgtgcag gcactggtgg ttcccggctc tggcccggta aaagcccagg
     9061 cggaagcgga aggtctggat aaaatattta ttgaagccgg ttttgaatgg cgcttgcctg
     9121 gctgctcaat gtgtctggcg atgaacaacg accgtctgaa tccgggcgaa cgttgtgcat
     9181 ccaccagcaa ccgtaacttt gaaggccgcc aggggcgcgg cgggcgcacg catctggtca
     9241 gcccggcaat ggctgccgct gctgctgtga ccggacattt cgccgacatt cgcaacatta
     9301 aataaggagc acaccatggc agagaaattt atcaaacaca caggcctggt ggttccgctg
     9361 gatgccgcca atgtcgatac cgatgcaatc atcccgaaac agtttttgca gaaagtgacc
     9421 cgtacgggtt ttggcgcgca tctgtttaac gactggcgtt ttctcgatga aaaaggccaa
     9481 cagccaaacc cggacttcgt gctgaacttc ccgcagtatc agggcgcttc cattttgctg
     9541 gcacgagaaa acttcggctg tggctcctcg cgtgagcacg cgccctgggc attgaccgac
     9601 tacggtttta aagtggtgat tgcgccgagt tttgctgaca tcttctacgg caatagcttt
     9661 aacaaccagc tgctgccggt gaaattaagc gatgcagaag tggacgaact gtttgcgctg
     9721 gtgaaagcca atccggggat ccatttcgac gtggatctgg aagcgcaaga ggtgaaagcg
     9781 ggagagaaaa cctatcgctt taccatcgat gccttccgcc gccactgcat gatgaatggt
     9841 ctggacagca tcggcctgac cctgcagcac gacgacgcta ttgcgtctta tgaagcaaaa
     9901 caacctgcgt ttatgaatta gaccccttgc ccggtcaaat gaccgggctt tccgctatcg
     9961 tccacgtcat caaacgtctt taacctttgc ggttaaaaat aatgcgacaa cagaaataat
    10021 cgcaattacc cagtagacag caaagtgccc ccaactttgt gcaattgctc cctgaataac
    10081 accagccaga attaccccgg tagagatagt gttggtaaat aaggtggtag ctgcccccgc
    10141 tcttccaggc attaaatcct gaaaccatag catcccaata cccgcaacaa tgccgataaa
    10201 tacagcgtta aaaagttgca gcgtcatcaa cgccatacgg ctatgaaaga aaatcaatcc
    10261 agtgtaaaac agcactcctg ccaccactgc aatgaccatc attcgccgct taccataacg
    10321 tttgacatag tagccagcca gaatcattgc tggtatttcc agtccagctg ccgtccccat
    10381 caggaaaccc gccagtttgt ctggcaatcc taaatcgcta ctgatccaca atggcatatc
    10441 aataatgtac atagtgttgc aggtccacat taacgtcgag gcgacaaata acatccgtac
    10501 gttactatcc tgccagtcac cttgcattga taaagcattt tccgacggca gttctacccg
    10561 cgccacagac agaagcataa atgcaatcaa taccagactg agtgtgaata tcccggcggc
    10621 aatcgaaaac atcactgtaa agccgtaatt caacgccagc ataaaggcca gcggtggacc
    10681 gattacccat gccagagaaa gctgcgcacg catcaccgag ctaaacatca ccacttctcg
    10741 cgccgagtta tccgcgtatt cccgcgccag agcaaataac tgtggcattg ccgtattggc
    10801 cagagatgcc agaagcacac cacaggtgat aagcgtcaga taatgacgat taaatgcaaa
    10861 cagtagcgca ttgccaatag ccatcaaaca gcaaaatata atcagttttc gccgatcgcc
    10921 ctgactgtca gaacgttttg ccaaccagag gcttacgccg atcccagcaa tagcattcac
    10981 cgtataaaag agtccgatcc agaaaggttg cgcgccaacc tcacgactca gaaataagct
    11041 caatgtagga gcctgtagcg ccccggccac ccccatcata aaagcgacca gcataaatgc
    11101 cgcgtaaaca ccgttcatac ggcgagccat cgtcattatc cagatcatac gttccctttt
    11161 tagcgcggcg agaataaaaa aaaccagcag gtataatctg ctggcgggtg attttacacc
    11221 aatactcagt cacacatgat gcaggcaagt caactttcag aattgcggtc atcccactgc
    11281 atcagtcctt caagcatggt taatcgttgt gggacgctta accaacgcaa ccagcacaac
    11341 ttcgctgtcg cggtaaaata gtgctgataa aactgacgca tggggcacct ccttgcttca
    11401 tcgttaggaa ttattgactt aatataggga aaataaaatt gctgtctttt gcgcaggagt
    11461 tcccctttta tgccatctgc tcgtctgcaa caacagttca tccgcctgtg gcaatgctgc
    11521 gagggtaaat cgcaggacac aacgctcaac gaactggcag cgttattgag ctgctcgcgt
    11581 cgtcatatgc gtaccctgct caacaccatg caggatcgcg gctggctgac gtgggaagcg
    11641 gaagtcgggc gcggtaaacg ctcgcgtctg acattcctct ataccgggct ggcgcttcag
    11701 caacagcggg cggaagacct gctggagcag gatcgtatcg accaactagt gcagttggtt
    11761 ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc tgggccgcag cttccgccag
    11821 gggcggcaca tcctgcgcgt gctctactat cgtccgttgc gtaatctgct acctggcagc
    11881 gcattgcgcc gttccgaaac ccatattgcc cggcaaatct tcagttcgct aacgcgcata
    11941 aatgaggaaa atggggaact ggaagcagac atcgcccacc actggcagca aatatcaccg
    12001 cttcactggc gtttcttttt gcgtccagga gtccattttc atcatggtcg tgaactggaa
    12061 atggacgacg tgatcgcctc tttaaaacga atcaatacgc tgccgctcta ttcgcatatt
    12121 acggacattg tgtcgccgac gccctggacg ctggatatcc atctcacgca gccggaccgc
    12181 tggttgccgt tactgctggg gcaagttccg gcgatgatcc tgccgtgcga atgggaaacc
    12241 ctcagtaact ttgccagcca tcctattggc accggtccgt atgcggtgat tcgcaacagc
    12301 accaatcaac tgaaaattca ggcattcgat gacttcttcg gttaccgggc attaatcgac
    12361 gaagttaacg tctgggttct gccggaaatt gccgacgagc cagccggtgg tctgatgcta
    12421 aaaggtccac agggcgagaa aaaagagatt gaaagccgcc tggaggaagg ttgctactat
    12481 ttactgttcg acagccgcac ccatcgcggg gcgaatcagc aagtcaggga ctgggtaagc
    12541 tatgtgcttt ctccaactaa tctggtctat ttcgctgagg aacagtacca gcaactgtgg
    12601 ttcccggctt atggactgct cccccgttgg caccatgccc gcaccataaa gagcgaaaaa
    12661 ccggctggcc tggaaagcct caccctgacc ttttatcagg atcacagtga gcatcgggtg
    12721 attgccggga tcatgcagca gattctggca agtcaccagg tcacgctgga aatcaaagag
    12781 atcagctacg atcagtggca tgaaggagag atcgagagcg atatctggct taatagcgcc
    12841 aactttacgc tgccgctgga tttttcgctg ttcgcgcact tgtgcgaggt gccgctgctc
    12901 caacactgta ttccgatcga ctggcaagcc gacgccgccc gctggcgcaa tggcgaaatg
    12961 aacctggcga actggtgcca gcaactggtc gccagtaaag caatggtgcc gcttatccac
    13021 cactggctga tcattcaggg acaacgcagt atgcgcggcc tgcgtatgaa taccctcggc
    13081 tggttcgatt ttaaatcagc gtggtttgcg ccaccggatc catgattgct ggtagataac
    13141 aaaatcacta cactaacgcc gttctcaacg gggtgccacg caaacgcgtg cgctgagaaa
    13201 atacccgtcg aacctgatcc ggataacgcc ggcgaaggga tttgaggctc cttctcaagt
    13261 cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa tgtcttcccc tgctgttgct
    13321 gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt tatacctacg attccttcgc
    13381 cgccgactgg gggcctggtc caaagatcaa aaaagctttt gaagccaact gtaactgcga
    13441 actgaaactg gtggcgctgg aagatggcgt ttcgcttctc aaccgtctac ggatggaagg
    13501 caaaaatagt aaagccgatg tggtgctggg gctggataac aatctgttag acgccgccag
    13561 taaaaccgga ctatttgcca aaagcggtgt ggcagcggat gccgttaacg ttcccggcgg
    13621 ctggaataat gacactttcg tcccgtttga ttacggctat ttcgccttcg tctatgacaa
    13681 gaacaaactg aaaaacccgc cgcaaagcct gaaagaattg gttgagagcg atcagaactg
    13741 gcgggtgatt tatcaggatc cgcgtaccag cacgccgggg ctgggtctgc tgttgtggat
    13801 gcaaaaagtc tatggcgatg acgccccaca agcctggcaa aaactggcga agaaaaccgt
    13861 cacggtcacc aaaggctgga gcgaagccta cggcctgttt ttaaaaggtg aaagcgatct
    13921 ggtactgagt tacaccacct ctccggctta tcacattctc gaagagaaaa aagataacta
    13981 cgccgccgcg aactttagcg aaggtcacta tctgcaggtg gaagtcgccg cccgcaccgc
    14041 tgccagcaag cagccggagc tggcgcaaaa attcctccag tttatggttt ctccggcttt
    14101 tcagaatgcg atcccaaccg gtaactggat gtatccggtg gcaaatgtca cgctgcctgc
    14161 cgggtttgaa caattgacca aaccggccac cacgctggag ttcacaccag ctgaagtggc
    14221 ggcacaacgt caggcatgga ttagcgaatg gcaacgcgcc gtcagccgtt aattcccggc
    14281 tggttaattc caggtgtaag cgccgccacg ctggtggtag cggttgcgct cgcggcgttt
    14341 ctcgccctgt ggtggaacgc gccgcagggt aactgggtgg cagtctggca ggacagctac
    14401 ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc tctcggcgct actctctgtc
    14461 gtacccgcga tattcctcgc ccgcgccctc tatcgcaggc gctttccggg tcggctggcg
    14521 ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc tagttgctgt tttcggcatt
    14581 cttagcgtct atggtcgtca gggctggctg gcatcgctct gccaatcgct cggtctggag
    14641 tggacctttt cgccctacgg cctgcaaggc attttgctgg cgcacgtatt ttttaatctg
    14701 ccgatggcga gccgcttatt actccaggca ctggaaaaca ttcccggcga acaacgtcag
    14761 cttgccgccc agcttgggat gcgcggctgg cattttttcc gcttcgtcga atggccgtgg
    14821 ctacggcgac aaatcccgcc ggttgccgcg cttatcttta tgctctgttt cgccagcttc
    14881 gccaccgtgc tatcgctggg cggcggtccg caggcaacca ctatcgagct ggctatttat
    14941 caggcgctga gttacgacta cgatcctgcc cgcgcggcga tgctggcgct gctccagatg
    15001 gtgtgttgcc ttgggctggt gctgttgagt cagcgactaa gtaaggccat tgcgcccggc
    15061 accacgctgc tgcaaggctg gcgcgatccg gacgatcgtc tgcatagccg catttgcgac
    15121 acggtgttaa tagtgctggc gctgctgctg ttgctgccac cgttgctggc ggtgatcgtc
    15181 gatggggtaa atcgccagtt gccggaagtg ctggcacaac cggtgctgtg gcaggcgctg
    15241 tggacctcgt tgcgtattgc gctggcggca ggtgtattgt gcgtagtgct gaccatgatg
    15301 ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa tgctggcggg tcaggcgctg
    15361 gagatgagcg gcatgttgat cctcgccatg ccggggattg tgctggctac cggcttcttt
    15421 ttactgctca acaacactat cggcctgcca caatctgctg acggcattgt gattttcacc
    15481 aatgcgttaa tggcgatccc ttatgcgctg aaagtactgg aaaacccgat gcgcgatatc
    15541 accgcccgct acagtatgtt gtgtcagtcg ctgggcattg aaggctggtc gcgcttaaaa
    15601 gtggtcgagc tgcgcgccct gaaacgtcca ctggcgcagg cgctggcttt tgcctgcgtg
    15661 ctgtcgattg gtgattttgg cgtagtggcg ttgttcggta acgatgattt ccgtaccctg
    15721 ccgttttatc tctaccagca aattggctcc tatcgcagcc aggacggcgc ggtcaccgcg
    15781 ttaattctgc tactactctg ttttctgctg tttaccgtga ttgaaaaact accggggcga
    15841 aatgttaaaa ctgactgata tcacctggct ttatcaccat ttgccgatgc gttttagctt
    15901 aacggtagaa cgcggcgagc aggtggcaat cctcgggcca agcggcgcgg gtaaaagtac
    15961 cctgctgaat ttgattgccg gttttctgac gccagccagc ggttcgctga ctatcgatgg
    16021 tgttgatcac accacgacgc cgccgtcacg ccgtccggtg tcgatgctgt ttcaggagaa
    16081 caacctgttc agccatctga cggtcgcgca gaacatcggg ctggggctaa atccggggtt
    16141 gaaactgaac gcggcacagc aggagaaaat gcacgcgatt gcccgtcaga tggggattga
    16201 taatttaatg gcgcggttac cgggcgagct ttccggcggt cagcgacagc gcgtggcgtt
    16261 agcgcgttgt ctggtgcgcg aacagccgat tttattgctc gatgaaccgt tctctgcgct
    16321 cgatccggcg ttacgtcagg agatgctgac gctggtgagc acgagctgcc agcagcaaaa
    16381 aatgacgcta ttgatggtgt cgcacagtgt ggaagatgcg gcgcggatcg ccacacgctc
    16441 ggtggtggtc gccgacgggc gcatcgcctg gcagggtaag actgaagaat tgttgagcgg
    16501 taaggcgagt gcttcggcaa tattggggat tacgggttag tatgcactgt cagatgcggc
    16561 gtgaacacct tatccgacct acggacagtg cgttttgtag gcctgataag acgcggtcag
    16621 cgtcgcatga ggcaacccgc acaagacaat cccctaaacc ccaaccactt tacgcaaaat
    16681 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
    16741 ggcagaaatc aacggcgtca gccacaacaa acggccgcgc gacaaataat gacttaaacg
    16801 atcggttgct ttaccgctgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
    16861 taccgccgtc gccagcagca accatttaaa ctcgccgctc tgcattccgg ctggaatatc
    16921 gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac ggtggccaca gcaggcagcc
    16981 gataatattc ggcgtaatga attttgccac cggcagatcc agcattcccg ccaccattgg
    17041 caccagcgga cgcgtcgggc caacaaagcg accgaccaga atggtgaaca tgctgtgttg
    17101 atgcaacgca tgttcagttt tatcgagtaa tgctttgttt ttcttcagaa atgaccagcg
    17161 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcagaca
    17221 accgacaatc cctgccagcc aagcgtgcca gaaacttaac tcgccgctgc caatcagcgc
    17281 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
    17341 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtagattggg taataaagtg
    17401 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
    17461 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
    17521 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
    17581 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
    17641 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
    17701 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
    17761 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
    17821 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
    17881 taatcgcttc catgcgccgc agtaacaatt gctcaagcag attcatcgcc agcagctccg
    17941 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatacg
    18001 gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa
    18061 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
    18121 cctccggatg acaaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
    18181 gtcggcagac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
    18241 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
    18301 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
    18361 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
    18421 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
    18481 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag caggaccaaa gccatgacaa
    18541 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
    18601 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
    18661 gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
    18721 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac
    18781 tgcgccagcg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcaaaaaggg
    18841 caattttgtg atgccccgaa taaccagttc cgtcatcatc cgcgtgacta cattgagtca
    18901 atggaagcgg cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg
    18961 gtcgggattg gcgttgacag taccggctcg acgcccgcac cgattgatgc cgacggtaac
    19021 gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg cgatgttcgt attgtggaaa
    19081 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
    19141 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
    19201 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
    19261 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
    19321 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
    19381 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
    19441 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
    19501 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
    19561 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
    19621 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
    19681 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
    19741 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
    19801 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
    19861 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
    19921 cgtcgcacgc caaacgctaa ccaacgcctg aaaggggtga ttaccgatct taacctcgct
    19981 accgacactc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
    20041 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    20101 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    20161 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    20221 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    20281 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    20341 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    20401 caggccgttg cgactctata aggacacgat aatggcgatt tttgataatt atgaagtgtg
    20461 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    20521 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctaca aactggtgtt
    20581 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    20641 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    20701 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt tcaacgcggc
    20761 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    20821 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtcg ttaccggtca
    20881 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgtcagg cggtctctaa
    20941 acaggatacc cgtcatctga aagtctgccg atttggcgat aacatgcgtg aagtggcggt
    21001 caccgatggc gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    21061 ggttggctat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    21121 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    21181 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    21241 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    21301 tctgcccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    21361 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    21421 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    21481 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    21541 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    21601 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    21661 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cactgtggaa
    21721 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    21781 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    21841 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    21901 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    21961 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    22021 aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg cgtcttatca ggcctacgcg
    22081 ctgcgatttt gtagaccgga taagcgaagc gcatccggca cgaaggagtc aacatgttaa
    22141 aagatctcaa acgccaggta ttagaagcca acctggcgct gccaaaacac aacctggtca
    22201 cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg cggcgtcttt gtgatcaaac
    22261 cttccggcgt cgattacagc gtcatgaccg ctgacgatat ggtcgtggtt agcatcgaaa
    22321 ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga cacgccaact caccggctgc
    22381 tctatcaggc attcccctcc attggcggca ttgtgcatac gcactcgcgc cacgccacca
    22441 tctgggcgca ggcgggtcag tcgattccag caaccggcac cacccacgcc gactatttct
    22501 acggcaccat tccctgcacc cgcaaaatga ccgacgcaga aatcaacggc gaatatgagt
    22561 gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca gggtatcgat gcagcgcaaa
    22621 tgcccggcgt tctggtccat tcccacggcc cgtttgcatg gggcaaaaat gccgaagatg
    22681 cggtgcataa cgccatcgtg ctggaagagg tcgcttatat ggggatattc tgccgtcagt
    22741 tagcgccgca gttaccggat atgcagcaaa cgctgctgga taaacactat ctgcgtaagc
    22801 atggcgcgaa ggcatattac gggcagtaat gactgtataa aaccacagcc aatcaaacga
    22861 aaccaggcta tactcaagcc tggttttttg atggattttc agcgtggcgc aggcaggttt
    22921 tatcttaacc cgacactggc gggacacccc gcaagggaca gaagtctcct tctggctggc
    22981 gacggacaac gggccgttgc aggttacgct tgcaccgcaa gagtccgtgg cgtttattca
    23041 cgccgatcag gttccccgcg ctcagcatat tttgcagggt gaacaaggct ttcgtctgac
    23101 accgctggcg ttaaaggatt ttcaccgcca gccggtgtat ggcctttact gtcgcgccca
    23161 tcgccaattg atgaattacg aaaagcgtct gcgtgaaggt ggcgttaccg tctacgaggc
    23221 cgatgtgcgc ccgccagaac gctatctgat ggagcggttt atcacctcac cggtgtgggt
    23281 cgagggtgat atgcacaatg gcactatcgt taatgcccgt ctgaaaccgc atcccgacta
    23341 tcgtccgccg ctcaagtggg tttctataga tattgaaacc acccgccacg gtgagctgta
    23401 ctgcatcggc ctggaaggct gcgggcagcg catcgtttat atgctggggc cggagaatgg
    23461 cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc agccgcccgc tgctgctgga
    23521 aaaactcaac gcctggtttg caaaccacga tcccgatgtg atcatcggct ggaacgtggt
    23581 gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt taccgtcttc cgctgcgtct
    23641 tgggcgcgat aatagcgagc tggagtggcg cgagcacggc tttaaaaacg gcgtcttttt
    23701 tgcccaggct aaaggtcggc taattatcga cggtatcgag gcgctgaaat ccgcgttctg
    23761 gaatttctct tcattctcgc tggaaactgt cgctcaggag ctattaggcg aaggaaaatc
    23821 tatcgataac ccgtgggatc gaatggacga aattgaccgc cgtttcgccg aagataaacc
    23881 tgcgctggca acttataacc tgaaagattg cgagctggtg acgcagatct tccacaaaac
    23941 tgaaatcatg ccatttttac tcgaacgagc aacggtgaac ggcctgccgg tggatcggca
    24001 cggcggttcg gtggcagcgt ttggtcatct ctattttccg cgaatgcatc gcgctggtta
    24061 tgtcgcgcct aatctcggcg aagtgccgcc gcacgccagc cctggcggct acgtgatgga
    24121 ttcacggcca gggctttatg attcagtgct ggtgctggac tataaaagcc tgtacccgtc
    24181 gatcatccgc acctttctga ttgatcccgt cgggctggtt gaaggcatgg cgcaacctga
    24241 tccagagcac agtaccgaag gttttctcga tgcctggttc tcacgggaga aacattgcct
    24301 gccggagatt gtgactaaca tctggcacgg gcgcgataaa gccaaacgtc agggtaataa
    24361 gccgctttcg caggcgctga agatcattat gaatgccttt tatggcgtgc tcggcaccac
    24421 cgcctgccgc ttcttcgatc cgcgcctggc atcgtcgatc accatgcgtg gtcatcagat
    24481 catgcggcag accaaagcat tgattgaagc gcagggctac gacattatct acggagatac
    24541 cgactcgact tttgtctggc taaaaggtgc acattcggaa gaagaggcgg cgaaaatcgg
    24601 tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa acgctgcaaa aacaacggct
    24661 gaccagcgca ttagaactgg agtatgaaac tcatttctgc cgttttctta tgccaaccat
    24721 tcgcggagct gataccggca gtaaaaagcg ctacgccggg ctgattcagg agggcgacaa
    24781 gcagcggatg gtgtttaaag ggctggaaac cgtgcgcacc gactggacgc cgctggccca
    24841 gcagtttcag caggagctgt acctgcgtat cttccgcaac gagccatatc aggagtacgt
    24901 ccgccaaacc atcgacaaac tgatggcggg tgaactggat gcgcggctgg tttaccgtaa
    24961 acgccttcgc cgtccgctga gcgaatatca gcgtaatgtt ccgcctcatg tacgcgccgc
    25021 tcgccttgcc gatgaagaaa atcaaaagcg cggtcgcccc ttgcaatatc agaatcgcgg
    25081 caccattaag tacgtatgga ccaccaacgg cccggagccg ctggactacc aacgttcacc
    25141 actggattac gaacactatc tgacccgcca gctacaaccc gtggcggagg gaatactccc
    25201 ttttattgag gataattttg ctacacttat gaccgggcaa cttgggctat tttgagcaaa
    25261 aaaaagagtt cgccagatac cattttgatg cgtgacgaat gctttgccat ccagtaccat
    25321 agcgcccttt ccattcctgg acctgaataa caccactacc tcataagcac ggtagcgggt
    25381 ggttattgcc tgcaattaaa gatatagagc cgaacacata tgccttttac acttggtcaa
    25441 cgctggatca gcgatacaga aagcgaattg ggacttggaa ccgttgtcgc ggtggatgcg
    25501 cgaactgtca ctttactttt cccatctact ggtgaaaacc gtctgtacgc acgcagtgat
    25561 tcccccgtga cccgcgtgat gttcaaccct ggtgatacca ttaccagcca tgacggctgg
    25621 cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc tgacctatat cggtactcgc
    25681 ctggatactg aagagtccgg cgtagccctg cgtgaagttt tccttgatag caaactggtg
    25741 ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg accgtatgga ccgctttgcg
    25801 ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc gtatgccgta cagcggcctg
    25861 cgcggtcagc gtaccagcct gatcccgcat cagctcaaca tcgctcatga tgttggtcgc
    25921 cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt tagggaaaac cattgaagcc
    25981 gggatgatcc tgcatcagca actgctctct ggcgctgctg aacgtgtgct gattatcgtc
    26041 ccggaaacct tacagcatca gtggctggta gaaatgctgc gccgtttcaa cctgcgcttt
    26101 gctctgtttg atgatgagcg ttatgccgaa gctcagcacg atgcttacaa cccgtttgac
    26161 accgaacagc tggtgatttg ctcgctggat tttgcccgtc gtagcaaaca gcgcttggaa
    26221 catctctgtg aagccgaatg ggacctgctg gtggtcgatg aagcgcatca cctggtgtgg
    26281 agcgaagatg cgccaagccg tgaatatcag gccattgaac aactggcaga gcacgtgccg
    26341 ggcgttctgc tgctgaccgc gaccccggaa cagctgggga tggaaagcca cttcgcccgt
    26401 ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc agttcgttga agagcagaaa
    26461 aattatcgtc cggttgcgga cgccgttgcc atgctgctgg caggtaacaa actgagcaat
    26521 gacgaactga acatgctcgg cgagatgatc ggcgagcagg atatcgagcc gctgttgcag
    26581 gcagcaaaca gcgacagcga agatgcccag agcgcccgtc aggagctggt ttcgatgctg
    26641 atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca cgcgtaacgg tgtgaaagga
    26701 ttcccgaaac gcgagctgca caccattaag ctgccgctac cgacgcagta tcagacggct
    26761 attaaagtct ccggcattat gggcgcacgt aaaagtgcgg aagatcgtgc tcgcgatatg
    26821 ctctacccgg agcgtattta tcaggaattt gaaggtgata acgccacctg gtggaacttc
    26881 gatccgcgcg ttgagtggct gatgggctac ctgaccagcc atcgctctca gaaagtgctg
    26941 gtgatctgcg ccaaagctgc cactgcgctg caactggagc aggtactgcg cgaacgtgaa
    27001 ggtattcgcg ctgcggtgtt ccacgaaggt atgtcgatta tcgaacgtga ccgcgctgcc
    27061 gcctggtttg ccgaagaaga caccggcgca caggtactgc tgtgctcaga aatcggttct
    27121 gaaggacgta actttcagtt cgccagtcac atggtgatgt ttgacctgcc attcaacccg
    27181 gatctactgg agcagcgtat tggtcgtctg gatcgtatcg gccaggcgca cgatattcag
    27241 atccatgtgc cttatctgga gaaaaccgct cagtcggtgc tggtgcgctg gtatcacgaa
    27301 ggtctggatg catttgagca cacctgcccg accggacgca ctatttacga tagcgtatac
    27361 aacgatctga ttaactatct ggcttcaccg gatcaaaccg aaggctttga cgatctgatc
    27421 aaaagctgcc gcgagcaaca tgaagcgctg aaagcacagc tggaacaggg tcgtgaccgc
    27481 ctgctggaaa tccactccaa cggtggcgaa aaagcccagg cactggcaga aagcattgaa
    27541 gagcaggatg acgataccaa cctgatcgcc ttcgccatga acctgttcga tattatcggt
    27601 atcaatcagg acgatcgcgg cgacaacatg atcgtgctga cgccgtccga tcatatgctg
    27661 gtgccggact tccctggcct gtcggaagat ggcatcacca tcacctttga tcgtgaagtg
    27721 gcgctggcgc gtgaagatgc acagtttatt acctgggagc atccgctgat ccgcaacggt
    27781 ctggatctga tcctttctgg cgataccggt agcagcacga tttcactgtt aaaaaacaaa
    27841 gcgttgccgg taggtacgct gttggtggaa ctgatttatg tggttgaagc ccaggctccg
    27901 aagcagttgc agctcaaccg cttcctgcca ccgacgccgg tacgtatgct gctggataaa
    27961 aacggcaaca acctggcggc gcaggtagag tttgaaacct ttaaccgcca gcttaacgcg
    28021 gttaaccgtc acaccggcag caaactggtt aacgccgtgc agcaggatgt tcacgctatc
    28081 cttcaactgg gtgaagcgca gatcgagaaa tctgcccgtg cattgattga tgcagcgcgt
    28141 aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc tggaagctct gcgtgcagtg
    28201 aacccgaaca ttcgtgacga cgaactgacc gccattgaga gcaaccgtca gcaggtaatg
    28261 gaaagcctgg atcaggcagg ttggcgtctg gatgccctgc gtttgatcgt tgtaacgcat
    28321 cagtaacgga gccgaaaatg gggatggaaa actacaatcc accgcaggaa ccctggttgg
    28381 ttatcctgta tcaggatgac catattatgg tggtcaacaa gccgagcggt ttgttgtcag
    28441 tgccgggtcg tctggaagag cacaaagaca gcgtgatgac gcgcattcag cgtgattatc
    28501 cgcaggcaga atcggtgcat cgtctggata tggctaccag cggcgtgatt gtagtggcgc
    28561 tgaccaaagc cgcggagcgg gagttaaaac gccagttccg cgagcgcgag ccgaaaaagc
    28621 agtatgtggc ccgcgtctgg gggcatccat cccccgcaga aggtctggtg gatctgccgc
    28681 tgatttgcga ctggccaaac cgcccgaaac agaaagtctg ttacgaaacg ggtaaacctg
    28741 cgcagacgga atatgaagtg gtggagtatg cggcggataa cacggcaaga gtggtgttaa
    28801 aaccgattac cgggcgttcg catcaattgc gtgtgcatat gctggcgctg gggcatccga
    28861 ttctcggcga tcgtttttat gcatcaccag aagcgagagc gatggcaccg cgtttgttgc
    28921 tacatgcaga gatgttgacg attacccatc cggcgtatgg caatagtatg acgtttaaag
    28981 cgccagcgga tttttaatcg tcacgctggc gcagttcggt aggcggataa ggcgttcacg
    29041 ccgcatccgg caattgtgcg ccgattgcct gatgcgacgc gggccgcgtc ttatcaggcc
    29101 tacagtttaa aacttattta aaccctttct gctgctttat cagctcatat gcctgctgaa
    29161 tttcctgcgc tttctgcttc gccatctcca tcatctcagg cggcaaacct ttcgccacca
    29221 gcttatcggg atggtgttca ctcatcagct tacggtaggc acgtttgatg gtggtcgcat
    29281 catccgtcgg cttcacgccc agcacattac aggcatcttc cagcgtcggg ccacgctgcg
    29341 cttgctgcca gttaccaccg ccagattgct gctgataacc gccgccaaac tgtgcaccgc
    29401 cctgcatcat gcgcaaaaac tggtcaaact gggcgcggga gatccctaat tcttcagcaa
    29461 tgacatacag caccgcccgt tcattcgggt gcagtgaacc atcagcaaac gccgcctgaa
    29521 tctggatctc cagaaacata cgaattaagt caaaacgacc aaagcagaca ctgcgaaact
    29581 ggcgcatctt ttcgcgcagc gggtaattat ctgattttcc cacccggaac gcgttttgcg
    29641 ccgcagtacg ggaagcgcca tgaaggttca ttcggtccat caactggctg gcgatatgaa
    29701 tatcagcctc cgtgacgcga cctttggatt tggttaaatg ccccatcact tcaaaagtgg
    29761 tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa ccacgccatt ttacggctac
    29821 gggctttatc aaacatatgg ccaattaaca ggcctaacac tacgccccaa aagccgccgc
    29881 ccatcagtaa ggccacggcc acgccaatga tttttcccca atactgcata tattccccaa
    29941 atcgacacac ggatatcagg gctatctccc acaatataaa ggtgctttta ccgttttccg
    30001 gctgcggtca attgtgggac atcgcctata atttgcatta tcatacctgt cattcaatgc
    30061 cgtgcctaac accactgacg ctattcggac aggattaaca ctagcgtaga gatgacgagt
    30121 acgttagtct ctgaccgttt gtcacgcgca acgttaccga tgatggaaca ataaaatcaa
    30181 cgtatgaaaa aacgtatccc cactctcctg gccaccatga ttgccaccgc cctttatagt
    30241 caacagggac tggcagccga cctcgcctca cagtgcatgt tgggcgtgcc aagctatgac
    30301 cgtcctctgg tacagggcga taccaatgac ttacccgtga ctatcaatgc tgaccacgcg
    30361 aaaggggact acccggatga cgccgtgttt actggcagcg tggatatcat gcagggtaac
    30421 agccgtctgc aggccgacga agtgcagctc catcaaaaag aggcaccagg acaaccggag
    30481 ccggtacgta ccgttgatgc gctcggtaat gtccattacg acgataacca ggtgatcctc
    30541 aaagggccga aaggctgggc gaatctgaac accaaagata ccaacgtctg ggaaggtgat
    30601 taccagatgg tgggtcgcca gggtcgcggt aaagcggacc tgatgaaaca acgtggcgaa
    30661 aaccgctata ccattctgga taacggtagc tttacctcct gtctgccggg ttctgacacc
    30721 tggagcgtgg taggtagcga aattattcat gaccgcgaag aacaagttgc tgagatctgg
    30781 aacgcccgct ttaaggtggg tccggtaccg atcttttata gcccctattt gcagttgccg
    30841 gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg ccaagtacac caccaccaac
    30901 tactttgagt tctacctgcc atattactgg aacatcgcgc caaatatgga tgccaccatc
    30961 acgccgcatt atatgcatcg tcgtggcaac atcatgtggg agaacgaatt ccgctacctc
    31021 tcccaggcgg gcgctggctt gatggaactg gactatctgc cttcagataa agtctatgaa
    31081 gatgaacacc cgaacgatga cagttcacgt cgttggttat tctactggaa ccactccggg
    31141 gtcatggatc aggtgtggcg tttcaacgtc gactacacca aggtcagcga tcctagctac
    31201 ttcaatgatt tcgataacaa gtacggttcc agtactgacg gctacgcaac gcaaaaattc
    31261 agcgttggct atgcggtgca aaacttcaat gccaccgttt caaccaagca gttccaggtt
    31321 ttcagcgaac agaacaccag tagctactcg gcagagccgc agttagacgt taattactac
    31381 cagaatgatg ttggtccgtt tgatacgcgt atttacggcc aggcagtgca ctttgttaac
    31441 accagagacg acatgcctga agcaacccgt gttcacctgg aaccgaccat caatttgccg
    31501 ctctctaata actggggcag catcaatacc gaagcgaagt ttctggcaac ccattatcag
    31561 caaaccaatc ttgactggta taactccaga aacacgacca agctggacga atccgttaac
    31621 cgcgtaatgc cgcaattcaa agttgacggc aaaatggtct ttgaacgcga tatggaaatg
    31681 ctggctccgg gttataccca aacgctggaa ccgcgcgcgc agtatttgta cgtgccgtat
    31741 cgcgatcaga gcgacatcta taactacgac tcgtctctgc tgcaatctga ctactctggc
    31801 ctgttccggg accggactta cggcggtctt gaccgtattg cctccgctaa ccaagtgacg
    31861 accggtgtca catctcgcat atatgatgat gctgccgttg aacgttttaa tatttccgtt
    31921 ggtcaaatct actatttcac ggagtctcgc actggcgatg acaacataac atgggagaat
    31981 gacgacaaaa cgggctcact ggtgtgggca ggcgacacct actggcgtat ctccgagcgt
    32041 tggggattgc gtggcgggat tcagtacgat acacgtctgg ataacgtagc gaccagtaac
    32101 tccagcattg aataccgtcg ggatgaagac cgtctggtac agctgaatta ccattacgcc
    32161 agcccggaat atattcaggc tacgctgcct aagtactatt ccactgctga gcaatataag
    32221 aatggtattt cgcaggtagg tgctgtcgcc agcaggccaa ttgccgatcg ttggtccatt
    32281 gttggggcct actactacga caccaatgcg aacaagcaag ccgactctat gttaggtgtg
    32341 caatacagct cctgctgcta tgcaattcgc gtcggttacg agcggaagct gaacggttgg
    32401 gataacgata aacaacatgc ggtatatgac aacgcaatcg gctttaacat cgaacttcgc
    32461 ggcctgagct ccaactacgg tctgggtacg caagagatgc tgcgttcgaa cattctgccg
    32521 tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc cgcagtgcgg ttaattgaaa
    32581 tggaaaaagt atgaagaact ggaaaacgct gcttctcggt atcgccatga tcgcgaatac
    32641 cagtttcgct gccccccagg tagtcgataa agtcgcagcc gtcgtcaata acggcgtcgt
    32701 gctggaaagc gacgttgatg gattaatgca gtcggtaaaa ctgaacgctg cgcaggcaag
    32761 gcagcagctt cctgatgacg cgacgctgcg ccaccaaatc atggaacgtt tgatcatgga
    32821 tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa atctccgatg agcagctgga
    32881 tcaggcgatt gctaacatcg cgaaacagaa caacatgacg ctggatcaga tgcgcagccg
    32941 tctggcttac gatggtctga actacaacac ttatcgtaac cagatccgca aagagatgat
    33001 tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc accatcctgc cacaggaagt
    33061 cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc agcactgagc tgaacctgag
    33121 ccacatcctg atcccgctgc cggaaaaccc gacctctgat caggtgaacg aagcggaaag
    33181 ccaggcgcgc gccattgtcg atcaggcgcg taacggcgct gatttcggta agctggcgat
    33241 tgctcattct gccgaccagc aggcgctgaa cggcggccag atggggtggg gccgtattca
    33301 ggagttgccg gggatcttcg cccaggcatt aagcaccgcg aagaaaggcg acattgttgg
    33361 cccgattcgt tccggcgttg gcttccatat tctgaaagtt aacgacctgc gcggcgaaag
    33421 caaaaatatc tcggtgaccg aagttcatgc tcgccatatt ctgctgaaac cgtcgccgat
    33481 catgactgac gaacaggccc gcgtgaaact ggagcaaatt gctgctgata tcaagagtgg
    33541 taaaacgact tttgctgccg cagcgaaaga gttctcgcag gatccaggtt ctgctaacca
    33601 gggcggtgat ctcggctggg ctacaccaga tattttcgat ccagccttcc gtgacgccct
    33661 gacccgcctg aacaaaggtc aaatgagtgc accggttcac tcttcattcg gctggcattt
    33721 aatcgaactg ctggataccc gtaatgtcga taaaaccgac gctgcgcaga aagatcgtgc
    33781 ataccgcatg ctgatgaacc gtaagttctc ggaagaagca gcaagctgga tgcaggaaca
    33841 acgtgccagc gcctacgtta aaatcctgag caactaatgg ttaaaaccca acgtgttgtg
    33901 atcactcccg gcgagcccgc cgggattggc ccggacttag ttgtccagct tgcacagcgt
    33961 gagtggccgg tcgaactggt tgtttgtgcc gatgccactc tccttaccga ccgggcagcg
    34021 atgctcggtt tgccgctcac cctccgccct tattccccca actcccctgc acaaccgcaa
    34081 actacgggca cattaacgct acttcctgtc gcgctacgtg aatctgtcac tgcggggcag
    34141 ttagcgattg aaaatgggca ttacgtggtg gagacgctgg cgcgagcgtg tgatggctgt
    34201 ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc ataaaggcgt cattaacgac
    34261 gcaagcattc cgtttaccgg tcataccgag tttttcgaag agcgttcgca ggcgaaaaaa
    34321 gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc tggcaacgac gcatttaccg
    34381 ctgcgcgata tcgcagatgc tatcacccct gcacttttgc acgaagtgat tgctattttg
    34441 catcacgatt tgcgtacaaa atttggtatt gccgaaccgc gcattctggt ctgcgggctg
    34501 aatccgcacg cgggcgaagg cggtcatatg ggtacggaag agatagacac cattattccg
    34561 gtgctcgacg agctgcgggc gcaggggatg aaactcaacg ggccgctgcc tgccgatacc
    34621 ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc tggcgatgta ccacgatcag
    34681 ggtcttcccg tgctaaaata ccagggcttc gggcgcggtg tgaacattac gctgggcctg
    34741 ccctttattc gcacatcagt ggaccacggc accgcgcttg aactggcggg acgtggcgaa
    34801 gccgatgtcg gcagttttat tacggcgctt aatctcgcca tcaaaatgat tgttaacacc
    34861 caatgaataa tcgagtccac cagggccact tagcccgtaa acgcttcggg caaaactttc
    34921 tcaacgatca gttcgtgatc gacagcattg tttctgccat taacccgcaa aagggccagg
    34981 caatggtcga aatcggcccc ggtctggcgg cattgaccga gccggtcggc gaacgtctgg
    35041 accagctgac ggtcatcgaa cttgaccgcg atctggcggc acgtctgcaa acgcatccat
    35101 tcttaggccc gaaactgacg atttatcagc aggatgcgat gacctttaac tttggcgaac
    35161 tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa cctgccttat aacatctcca
    35221 cgccgttgat gttccatctg tttagctata ctgatgccat tgccgacatg cactttatgt
    35281 tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa cagcaaagcg tatggtcgat
    35341 taagcgtcat ggcgcaatac tattgcaacg tgatcccggt actggaagtt ccgccgtcag
    35401 cctttacacc accacccaaa gtggattccg ccgtcgtgcg cctggtccct catgcaacga
    35461 tgcctcaccc ggttaaagat attcgcgtgt tgagccgcat caccaccgaa gcctttaacc
    35521 agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt tagcgtcgag gtgttaaccg
    35581 gaatggggat cgacccggcg atgcgagcgg aaaatatctc tgtcgcgcaa tattgccaga
    35641 tggcgaacta tctggcggag aacgcgcctt tgcaggagag ttaacgatga tcaattcgcc
    35701 ccgagtgtgt attcaggttc aaagcgtcta cattgaggca caatcttcac ctgataatga
    35761 acgttacgtt tttgcttata ccgtaaccat acgcaatctg gggcgagcgc cagtgcagtt
    35821 gttggggcgt tactggctga tcaccaatgg caatggccgt gaaaccgaag tccagggcga
    35881 aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa gagtaccagt acaccagcgg
    35941 tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac tacgaaatga tcgatgaaaa
    36001 tggcgtccct ttcagcatcg acattcccgt attccgactc gccgttccca cactcattca
    36061 ttaaaagaat atggcgacat accttattgg cgacgttcat ggttgttacg atgaactgat
    36121 cgcattgctg cataaagtag aatttacccc cgggaaagat accctctggc tgacgggcga
    36181 tctggtcgcg cgcggcccgg gttcgctgga tgttctgcgc tatgtgaaat ccttaggcga
    36241 cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg ctggcggtat ttgccgggat
    36301 cagccgcaat aaaccgaaag atcgcctgac accgctgctg gaagcgccgg atgccgacga
    36361 gctgcttaac tggctgcgtc gccagcctct gctgcaaatc gacgaagaga aaaagctggt
    36421 gatggcccac gcagggatca cgccgcagtg ggatctgcag accgccaaag agtgcgcgcg
    36481 cgatgtagaa gcggtgctgt cgagtgactc ctatcccttc tttcttgacg ctatgtacgg
    36541 cgatatgcca aataactggt caccggaatt gcgggggctg ggaagactgc gttttatcac
    36601 caacgccttt acccgtatgc gtttttgctt cccgaacggt caactggata tgtacagcaa
    36661 agaatcgccg gaagaagccc ctgccccact gaaaccgtgg tttgcgattc ctggccctgt
    36721 cgctgaagaa tacagcatcg cctttggtca ctgggcatcg ctggaaggca aaggtacgcc
    36781 ggaaggtatt tacgcgctgg ataccggctg ctgctggggt gggtcattaa cctgcctgcg
    36841 ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg cataaggatt tgggcgaagc
    36901 ggcggcgtct taaacacagc ctgatacagg aaggccggat aagacgcgac cggcgtcgca
    36961 tccggcacta gccgtaaatt ctatacaaac ttaccgccgc tccagaatct caaagcaata
    37021 gctgtgagag ttctgcgcat cggcgtcgtg gaattcgctg aataccgatt cccagtcatc
    37081 cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct gcgtcgatat gcgtcagata
    37141 cagtttttgc gcttttggca ggaactgctc ataaacgcga ccgccgccaa tcaccatgat
    37201 ttctggtacg tcaccacacg ccgcaatggc ttcatccacc gatttcaccc acgttacgcg
    37261 atcgtccgta cccggttgac tgctgaggat aatatttttg cgtcctggca acggacgacc
    37321 gattgattcc caagtatggc ggcccataat cacgggttta tttaaggtgt tgcgtttaaa
    37381 ccaggcgaga tcggcaggca ggttccacgg catggcgttt tccatgccga taacgcgatc
    37441 taccgctaac gccgcaatca gactgatcat tgagatttcc cgataaaaaa attgtcgcca
    37501 ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga tgatgttgag gaaaatttta
    37561 tattctgctg gcgagtccac gctctccccc tggactcgcc gcattacaat gaaacaaaaa
    37621 caaacagtta gctgtaaagt gtgatttacg tcactcttta ttaagatgag ggtttcgttt
    37681 ccggttcatc cgccatgttg ccggtatgtt taccttcttc tgttccctgc cagccatgtc
    37741 gttgaattaa tgacagatgt tcgcggtcct cggtgatgat ctcacttaac atcgcgctgg
    37801 tgcgtttata gaccgccgcg cgggctttgg tgtcattctc aaccattgcc atctcttcca
    37861 ccatctgaat attaaagcgg cggaacacat cggcacgttc tcgcgcttca tacggcccca
    37921 gacctaaact ttccagcgcc agacgcccgg ttttcagcgc accttcaaag gtttcacgct
    37981 ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg atcgacatcg cgggcgcggg
    38041 caataatctg caaatgcggg aaatgttctt tcaccatctc cgtcagttgc aggttggttt
    38101 gcggatcgtc aatagcgtta atcagcactt ccgctttcgc cgctccggca gattccagta
    38161 aatccatccg cgtggcatcg ccataaaaca ctttcatacc aaatttacgc agcgtttcga
    38221 tatggtccgg atcgtgatcg agcaccacca ttttcacccc gctggagagc agtaaacgtc
    38281 cggtaatctg tccaaaacga ccgaatccgg cgataatcac gcgcggctgt tcttcatcga
    38341 tctcatcggc ttcacgcgcc tcctccgtag aagattgctc aaggcgattg aggatcacca
    38401 gcagaatcgg cgttgctgcc atcgacagcg ccaccgccag ggtcagcgat ttcacccact
    38461 ccggctccag cacattcgcc atctgcgccg caccaaatac cacaaaggca aactcactgc
    38521 cctgccctaa caacaccgca aaccagcgac gctgtttatt tggcacctgc aacggtcggg
    38581 caatcagcca cagcatggcg attttgatga tgaggaaacc gagcagcaaa atgacaatgc
    38641 gcaatgggtt ttcaatcagc gtgccaaagt ctatcgacat gccaacaccg atgaaaaaca
    38701 gccccaacag caaaccttta aacggttcga tatcgctttc caacgcatga cggtattccg
    38761 agcttgccag cagtacgccc gccagaaacg cgcccatcgc catcgacaag ccgacctctt
    38821 ccagcagcaa accaaagcca aacacaagga ataacgccac ggcactaaac acttcccgca
    38881 agccagagcg ggcaacaaaa cgcagcgccg gacgcgtaac atagcgcccc agcaatacca
    38941 ccagtaccag cgcacccgcc acttttaacg ccgagagagc aaatgcgccc atcgtcgtcg
    39001 aggcactgct cgccgccagt agcgggatca tcgccaccag cgggattgcc gcgatatcct
    39061 ggaacagcag cactgcaaag gcactgcggc ccatttgcgt caccatcaga ttgcgttcat
    39121 tcatcgcctg catggcaatc gccgttgatg agagcgccag cgtcataccg atcaattccg
    39181 cgacctgcca gcgcagccca agtaacatac agaacagccc cagcaggcca ccgcaaatca
    39241 ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag cttccacagc ctttgtggat
    39301 cgagttcgag gccgataata aacagcatca gcaccacccc aatctcggca aagtgcagaa
    39361 tagattcggc atcggtcacc agtcgcagcc cccacgggcc aataatgcag ccggcgatca
    39421 ggtagccaag taccgatccc agaccaagac gtaccgcaat gggtacaatc agcgccgccg
    39481 atccgaggta gatcagcgcc tgaaccagcg tatgactatc catgatgggc ctcctgccat
    39541 tccagcagac gttgcttata gtggcgcgcc tgcccttcga gggtttcgtc gtcacaaata
    39601 aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc cgcagtagat tgccgtcgcc
    39661 tgtagcggct gcgacagcac atcaaagccc ggatgcgcac caatttcaaa atggctttcc
    39721 ccgccgccgg tcgtcaccgc ccacagcaaa tgtttgccat gcagcgccgt gccgccgtga
    39781 ccgtaagccc agccgtgcga caaaacttta tcgatccaaa gtttaagaag cggaggaatg
    39841 ctgtaccact gcatcgggtg ctgccagacg atcagatcgg cgcgagagag cgcctcctgc
    39901 tcggcggcaa tatcgatatt gaagtcagga tagagttgat aaagagagcg aatttcgacg
    39961 ccttccagcg tccttgcctg ttcaagcatc cgtttattcg catgggaatg atgcggatac
    40021 ggatgcgcat aaatgatgag gatcatagtt attagcccgc gtgtttacgc ctttgttttc
    40081 ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa taatattgat tcagaaaaga
    40141 gagaaatctg aacaaataat cccattctct gcccgcggat cgtcatattg ctggtctgcg
    40201 ccagtgacat tcctcgagtc tccggggcaa acgctacgga aatcaacaag ccaaacagcg
    40261 agatacccgc ccccattagc atcgtgttac tgataccgta attattgata aatatcggca
    40321 gtgcccaggt cgaaacaatg gtgccaatac gacttaagga cataatcacg cccacggcag
    40381 aggcgcggat atccgtcggg aagagttcat taggatagag ccactgcaaa ttacccggcc
    40441 cgccagagaa aaaggcatac accgcaaagg ccatcactac cagccagatc cccatatccg
    40501 ggattagccc caaaaccgcc aacgccagcg tcatcatggc aaagctgcca atcaacaatg
    40561 gacgccgtcc ggcagtgttt agccacagca tcggcggaat acagccgagc ataaagaaca
    40621 ggctaatcac cacattccct agtgccgcgt ttttgccaac ccccaatccc aacgaaccaa
    40681 cgatttgcgg gccaaaggtg taaatggcga acattgggat cacctggcag gtccagatgg
    40741 cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt
    40801 gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagttttatc atcatctctt
    40861 cgcactcttt tactcgccct ttgcgtaata accagcgggg agattctggc agttcgaatc
    40921 gaccaatcaa aatcaacaaa caagggatcg ccgcgctacc cagcatccag cgccaaccac
    40981 cttccacatc ataaagccag tagccgacca gatcggcgca ggtcgcgccg acataccaca
    41041 tcgcggcaat aaagctgatg gaaaacgccc gctgacgggt actggagaac tcggtgatca
    41101 tcgaagtggc gatgggataa tctgcaccga tgacaatgcc gatgagtacc cgcatcacca
    41161 acagttcgac gggggatgaa acacacatcg tcgccaccga tatcacgccg atggcgatga
    41221 tatcaatgag gaacattttg cgccgtccga ctttatcgga gatatagcca aacagcgatg
    41281 tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa gccaatccag tcagcgtcca
    41341 gtttcagcgc cggagtcagt tgctccagcg ccacgccaat cattaccagt acataaccat
    41401 ccagaaacgg accaccgctt ccccacagca aaatgcggcg gtgaatagag gagaatttga
    41461 gatcgtcaaa gtttctggac ggttgcatgg gttattcctg tttttttgca atgagagtca
    41521 gtacggcgtt acgtatcaaa ccaacatcag ccgtaacgga actccacacc aaaggtgccg
    41581 cgcgggtatt cccactgttc cagcgccgac cccagcccca gaatgcgaca ggtgccgcac
    41641 tccagacatc cggcgtaatc gaagcgcaca ctgccgtcat cctgcttctt gtacagacct
    41701 gcggggcacg ctttcaccag cagctccagc acctgtttat cagcatcggc cttcacaaca
    41761 atgtgcggat gctcttcatc gacattgaat ttattgacgc ccagtttgac gtccacattg
    41821 acgggagaag tcataaaacg gtcactcctt tcatgccatc cttgatcaga ttgatgaagc
    41881 ccactttctt gccgtggcgg aggattttct tgcgcatcag ttccggcgca ctgccgtcaa
    41941 tggtgaacag gtcacgcgcc acgcccaccg ccagttccgg gttgccgcta aacatgcgtg
    42001 ggttatcaag gaacgccggt agtttctggt acatgcgcat atcgcgcagc gggccactct
    42061 ccagatgctg acgatattcc gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg
    42121 aaagcacggt ttttgctgca gcttccccgg cggcaatcgc cagatccata ccgcgaatgg
    42181 taaaaccgag gttcatacac attccggcgg catcaccggc aatcaatacg ccgtcaccga
    42241 ccagctccgg cagcatgttg atgcctgctt ccggcactac gtgagcggaa tattccacca
    42301 gcttgccgcc cgcgatcagc ggtgcaacgg ccggatgctg tttgaaatct tccagcattt
    42361 gcggcaccga ttttttcgcg tcatgcagat gatgcagacc acaaaccagc cccagcgaca
    42421 gggtattttc attggtataa aggaagccgc cgcccatcag gccatcggtg ggtgatcccg
    42481 caaacagaca agccgcgccc tgattaccct gcaactgaaa acggtcttca attaccgact
    42541 tcggtaactc gatcagttcc ttcacgccaa ccgccacatc cgtcggttta acgcgttttg
    42601 ccatccccaa cttttcggca aggatggagt tcaccccatc agcaaggagc accgttttcg
    42661 cttcaatcac atcaccatcg gcttctacac cgacgacttt gccatcgcgc tgtacgaggt
    42721 tatcgacgcg gatcccggta attaactgcg cgcccgcttc ttcggcctgc tccatcaacc
    42781 aggcatcaaa tttactgcgc aaaacggagt aagaacgctg ggatggcgag gtttcgtcac
    42841 cgttgcagta gtccatagtc atcgctgact tttccgtcat aaacgcgagt ttttcatggg
    42901 tgatcaggcg ttctacgggg gcggaatcgg cgaaacctgg aataatgtgt tccagactgt
    42961 gggcatagag acgcccgcca gtaacgttct tggcacctgc ggaattgcca cgctcgataa
    43021 ctaacacttg cgccccttcg cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg
    43081 caccgacgat gatggcgtca aagatatctt cggacataag agctccctgt cagcggcaga
    43141 gccgtaatgc agccctgcca gagtggatca acgcgctaaa gctgcggtca gtgccggaag
    43201 gatcttcacg gcatcgccaa caatgccgta atccgcgtac tggaagatcg gcgcattttt
    43261 atctttattg atggcgaaaa tggtttgcga cgcgttagcg ccaaccatgt gctggatcta
    43321 cccggagatc cccaccgcca ggtacagttc aggtttcagc atcaggttgg agataccgac
    43381 atagcgttcg tgctccatcc atttttcgtt ttctgccacc ggacgagaac aggccaactc
    43441 cgcacctatc gccttgcaaa gctgttctgc cagcgcaatg ttctctttgc tgccaatgcc
    43501 gcgaccgacg ctgaccacca gacgggcttt gtcgagatcg acgctgttgc tctggcgcgc
    43561 ctgggttgcc gtgcgggtaa tcgccacagc cggagcctgc cactccacgg tgtgcgtttc
    43621 gccagtgcgt gacgcgtctg gctgagccgc atcgaacgtg ccgctgctga tggtcagtac
    43681 cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga ccgccgtaaa ccatgtgttt
    43741 cactgttgct ttaccgtcct gtacgctgac ggtgctggca tcgttagaca ccgccgcttt
    43801 aaggcgataa cccagttttg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac
    43861 cagaccgtca gcgccgtgct ggcgaatagt gtcagccatg acaccggcgt aatcttcgat
    43921 catccgatcg tccggtttgc cgtttaattt ccagacatga ttagcgccga gctggattgc
    43981 ctgtgcgccg tcggcatcat tgaggacaaa ggcattgatt tgattagcta aagcctgcgc
    44041 accgttcatc agttccggca gacgagaggg ggtatcgctg aatacccaga cttgagaaaa
    44101 cgtgttcata gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg
    44161 atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg
    44221 gcaacctgtt gttcagacca ggcgtccacg gcgttaaaac caatatccgc cgccgaccat
    44281 acctggactg gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag
    44341 ttgatatcag tggaaacagc aacaaccgca ggcagcggaa tgcttaaggt ttcggtttca
    44401 tcttccagtt cgcgctcaac ggtgagggta tctgccgtca gggagataat tttgctgacg
    44461 ccgttaactg ccggaatatt gaggatttcg cccaccagca gaccaacctg ctgggcataa
    44521 aggtcggaag aaccatcgcc acagaggatc agatcaaagc ctgctttctg ggcggctgca
    44581 gccagtgcgc tcgccgtttg ttgcggcagt gcctgctcga actggtcatc aatcaccaca
    44641 atcagttcat ccgggccgcg cgatagcaca tctttacgcc ctttggcgtt ggtcagggct
    44701 ttaccgccca cacttaaggc tgtcacctgc gcctctgctg cctgttgctt tagctggcaa
    44761 gccgcttcaa tagcgttgag atcgtattgg cttattttgg catcggcttt gctgaagtct
    44821 aatgaaccat cagcattatt gaccgcaata tcctgttcat caggcacgca cttatagcaa
    44881 gtaataatct tcattgcatc tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg
    44941 ttaattacag atatcacgcc ctttaatttg gaaataaaac tttaatcacc aatattgaaa
    45001 gtcagatgct atttttttac ccacaaaaga atataacaaa ctgattaata accaaaatta
    45061 acgaaaaacg ccctctgttc actcgaaaca aaaatgtgat accaatcaca gaatacagct
    45121 tattgaatac ccattatgag ttagccatta acgcgtccac gatgttaata ataattatat
    45181 taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt ttcacagtgg
    45241 aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt tttctttccg
    45301 ccattaataa tcgtcggcat actttgttgg cttacagtca gagatctgga tgcggcgaat
    45361 gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc atttgaatgg
    45421 tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta tgccaaaaag
    45481 cgtttaggta acgaaccgcc agaatttagc accgccagtt ggatctttat gatgttcgcc
    45541 tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta ctacatctcc
    45601 accccgccgt ttggcttaga accgaactcg acaggagcga aagagttggg gctggcttac
    45661 agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc agtcgccttc
    45721 gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac actggtgccg
    45781 ctggcaggtg aaaaacacgc caaagggttg ttcggtacca tcgtcgacaa cttctatctc
    45841 gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct ggtgaccgag
    45901 tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat catcattacc
    45961 tgctggatta tcctcaacgc catttgcgtc gcttgcggtc tgcaaaaagg ggtacgtatc
    46021 gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttgggtgtt catcgtcagc
    46081 ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct gatgtatctg
    46141 ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca gggctggacc
    46201 gtgttctact gggcatggtg ggtgatttac gccatccaga tgagtatctt cctcgcccgc
    46261 atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgatggg gctgacagca
    46321 tccacctgga tcctgtggac cgtgctcggt agtaacactc tgctgttgat ggataaaaac
    46381 atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat catcgaaacc
    46441 tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct ctgctttatt
    46501 gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtctac ttgccgcgaa
    46561 gtacgcgatg gtgaagaacc accgctgctg gtgcgtatcg gttggtcaat tctggttggc
    46621 attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac cgccattatc
    46681 gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt tattaaagac
    46741 gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg aaagatggat
    46801 tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact gatggccagc
    46861 gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt
    46921 gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga gcacggtggt
    46981 ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg tctgggggca
    47041 ccaacctatg tactgtacca gctgccgggc gggttcaata ccttcctgcg cgaaggcaca
    47101 caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat gtggaactca
    47161 gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac ttatacccgt
    47221 agaaatggta agatttatct taatggtagt aagtgtttta ttaccagtag cgcctacacc
    47281 ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa
    47341 tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgagaa gctcggtctg
    47401 cgtatggata gctgctgtga aatcaccttt gacgatgtgg aactggacga gaaagacatg
    47461 ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc
    47521 ctggtagccc tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc tgcgcgctac
    47581 gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat tcaggaaaaa
    47641 ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga agcagcgtgg
    47701 aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata cttctgcgcc
    47761 aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg
    47821 ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt ttccggagga
    47881 tctgacgaaa tgcagatcct gacgctgggt cgtgcggtgc tgaagcaata ccgctaagtc
    47941 cgtgcgctgc ctgatgcgac gctgacgcgt cttatcaggc ctacgggagc acgaatgtaa
    48001 gccggataag gcgtttacgc cgcatccggc agtcatgcgc tacataaatt tttcaggagc
    48061 taataatgga tcatctaccc atgccgaaat tcggaccgct ggccggattg cgcgttgtct
    48121 tctccggtat cgaaatcgcc ggaccgtttg ccggacaaat gttcgcagaa tggggcgcgg
    48181 aagttatttg gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc
    48241 cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc
    48301 gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc agtaaaggtc
    48361 cggcctttgc ccgtcgtggc attaccgatg aagtactgtg gcagcacaac ccgaaactgg
    48421 ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc aatcttccgg
    48481 cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt gatgttgacc
    48541 agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc gccaccacgg
    48601 cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt atcgacatcg
    48661 ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac ttcaacggcg
    48721 gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt tgcggtctgt
    48781 ataaatgtgc cgacggctac atcgtgatgg aactggtggg cattacccaa attgaagagt
    48841 gctttaaaga tattggcctc gcacatctac tcggtacgcc ggaaatcccg gaaggcactc
    48901 agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa ctcgatgcct
    48961 ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg aatatcgcct
    49021 gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat
    49081 caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac atcatgccga
    49141 aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc atggacacgg
    49201 ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg gtcagcaaag
    49261 gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg
    49321 cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg cggacaacat
    49381 ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc gctgatttgt
    49441 gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca ggagattaac
    49501 cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcaacaaggt tgcactacat
    49561 ctcgacaact gcccggaatt tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt
    49621 atggtgccga ttaacgcccg cctgttacgc gaggaaagcg agtggatctt gcaaaatagc
    49681 caggcgtgcc tgctggtgac cagtgcgcaa ttttatccta tgtatcaaca gattcagcag
    49741 gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc cgctgatgat
    49801 ggagtgagtt catttactca actgaaaaat caacaacctg ccaccttgtg ctatgcaccg
    49861 ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg
    49921 aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc cgcctggcag
    49981 tgtgcactgc gtgacgatga cgtctacatg acggtaatgc ctgcgtttca tatcgattgc
    50041 cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct ggtcgagaaa
    50101 tacagcgccc gcgccttctg gggacaggcg cagaagtacc gcgccaccat taccgaatgt
    50161 attccgatga tgatccgtac gttgatggtg cagccgcctt cagcgaacga tcggcaacac
    50221 cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga cgcattttgt
    50281 gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat tgtgggcatt
    50341 atcggcgatc gccctagcga taaacgacgc tggccgtcga ttggtcgggc ggggttttgc
    50401 tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa
    50461 atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct caacccgaaa
    50521 gccactgcga aagtgctgga agccgacggc tggctacata ccggcgatac cggataccgc
    50581 gacgaagaag gcttttttta tttcgtcgat cgccgctgca atatgatcaa acgcggtggc
    50641 gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccgaa aattcaggac
    50701 atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc atttgtggtg
    50761 ctgaatgaag gtgaaacatt gagcgaagag gaattttttc gcttctgcga acaaaatatg
    50821 gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg taattgctcg
    50881 gggaaaataa ttagaaagaa tctgaaataa atgaaacgtc agggaactac cctgccagct
    50941 aataatcacg ccctaaaaca atatgcattt tttgcaggga tgttgtcgtc cctaaaaaag
    51001 caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg atcaattctg
    51061 gaaattaccc ttgatcgccc aaaagcgaat gctattgatg caaaaaccag ctttgaaatg
    51121 ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat tattaccggt
    51181 gccggagaga aattcttttc cgcgggctgg gatttaaaag cggcagcaga aggcgaagca
    51241 ccggatgctg actttggccc gggtggtttt gcgggattaa ccgaaatttt caatctcgac
    51301 aaaccagtta tcgcagctgt gaacggctac gcctttggcg gcggtttcga actggcgctg
    51361 gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga agccaaactg
    51421 ggcatcgttc ctgacagcgg cggtgtgctg cgtctgccga agatcctgcc gcctaccatc
    51481 gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct gcgttggggg
    51541 atagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga actggctcag
    51601 cagctggtta acagcgcccc gctggcgatt gcggcgctga aagagatcta ccgcaccacc
    51661 agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt gaaacactat
    51721 ccatcggttc tgcattcgga agatgccatt gaagggccgc tggcgtttgc cgagaagcgc
    51781 gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag gggttaattc
    51841 cggtggttca cccgacggcg tttgtccatc ccagtgccgt cttgattggc gatgtgattg
    51901 tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac gggcggttga
    51961 tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac tgcgacactg
    52021 acaccatcgt tggggaaaac ggccatatcg ggcatggagc gatcctgcat ggttgtgtga
    52081 ttggtcgcga tgcattggtc gggatgaaca gcgtaattat ggatggcgcg gtcattggcg
    52141 aagagagcat tgttgccgca atgagctttg tcaaagcagg ctttagtggc gagaaacgcc
    52201 agttgttgat gggtacgcca gcccgcgctg tacgcagtgt tagtgacgac gagttacact
    52261 ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac
    52321 atgaaacgca gccactgagg caaatggagg aaaatcgccc ccgtttgcag gggacgacgg
    52381 atgtgacgcc taaacggtga tcttgccgga tgcgacctgg ttgctggata agatgctaca
    52441 acatcgtatc tggcaagcag gacgcccggt atcaacgaga taaaattaac gacgcatact
    52501 ctttgacaac atcatctgcc acttctgctc gcggttaagt tccgtgagcg gaacggcagg
    52561 aatacgcggt gtctttctta cgccgactaa tttttcccga ctgttattgc gcaggcgcgc
    52621 gtaaatttgc tcatcgatat cgaccacttt aaccagtcgc tgacactggc atccccgccc
    52681 ttccagctta ttagggatca tcttaacttc gcagcttatt tctgtgactt ccgacagaat
    52741 ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag agatctctct
    52801 tgctatcacc caccgatttt cagccatcat ccattcggcg attaacaagt agagtggttt
    52861 ttcaacatat ccttcacaca tacgaactcc agtttcacaa attaaaagtc tagctatgac
    52921 tcgttttgaa gcaattaaac aaggccatat taaaattgtg gatatatcga tagtatgcaa
    52981 cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc atcaaaaata tcgattcccc
    53041 aaaagattta cttaatgggc aaaaaaaaac ggtcctcatc agagaaccgt attagttatt
    53101 cgatcaaatt agcggatgaa aaatatccgc cacgacacgc tattatttga tctgtgcgtg
    53161 catttcctgc accgaaatta ctttttcagt cgcatcggca ttcagcgcca tcgcggtagc
    53221 gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac tgcgacggat
    53281 cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg ttgatgatgt aggtatattc
    53341 gccattcttg atacggtcct gaatgtgcgg acggccttca tgcaccttgt ttaccagacg
    53401 cggattgata cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa
    53461 gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac
    53521 ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc
    53581 gaacgcttca gcgaaggtgc ggcccacgcc catgacttcc ccggtagagc gcatttctgg
    53641 ccctaacagc gggtcaacgc ctgggaattt attgaacggc agcaccactt ctttcaccga
    53701 gtagtacggc ggaataactt ctttggttac gccctgctca gccagcgatt tgccagccat
    53761 cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga acggaacagt
    53821 acgcgccgca cgcgggttta cttcaatcag gtaaacttcg ttgtttttca ccgcaaactg
    53881 cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct gctggcgcat
    53941 cacatcctga atttcctgac ttaaggtgta cgctggcagc gaacatgcgg agtcaccgga
    54001 gtgcacaccc gcctgctcga tgtgctccat gatgccgcca atcagcacca tttcgccgtc
    54061 gcagatggca tccacgtcaa cttctactgc gtcatcaagg aaatgatcca gcaacactgg
    54121 cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata
    54181 gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgcaccacca gcgggtagcc
    54241 aatctctttt gccttctcaa ctgccatttc aatagcagta acggtggcgt tcgccggttg
    54301 tttcagtttc agacggtcaa ccgcatgctg gaagcgttca cggtcttccg cacggtcgat
    54361 agcatccggg ctggtgccga taaccggtac gccagcggct tccagtgcac gcgccagttt
    54421 cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa tacgcacgat
    54481 ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc
    54541 ggtagagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt cttcacgcag
    54601 cgccagcgag gcgtgtacgc agcagtagtc gaattcgata ccctgaccga tacggtttgg
    54661 accgccgcca agcaccatga ttttttcacg gtcggtagac ggattcgctt cgcactcttc
    54721 ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac tctgccgcac aggtatccac
    54781 gcgcttgtag accgggtgca ggtcatattg gtcacgcagc ttacggattt ccgcttcgcg
    54841 tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct ttgcgtttca gctggcgcag
    54901 gaattcagcg ttcaggccag tgatgcccac ttccgccact ttctcttcca gacgcaccag
    54961 ttcttcaatc tgtaccagga accagcggtc aatgttagtc aggttgaaga cgccgtccac
    55021 agacaggccc gcgcggaaag catcggcgat gtaccagata cgctctgcgc ctgcgtcttt
    55081 cagttcgcga cggattttgg ttaacgcttc cgggtcatcc aggctcactt tcgggtcgaa
    55141 gccagttgcg cccacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg
    55201 accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc
    55261 ggcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc
    55321 gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcta gggtgtaacc
    55381 caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc
    55441 cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt
    55501 caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca gcaccgccat
    55561 cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg gcgcgacagt
    55621 gatggagtca ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat
    55681 gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga
    55741 ctcatcaatc agcaactctt tggtcggaga gagatccaga ccgcgggcgc aaatttcttc
    55801 aaactcttca cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg
    55861 aataatgcac gggaagccca cgtcagcggc aacagccagc gcttcttcca tcgtgtgtgc
    55921 gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga aacgacggcg
    55981 gtcttctgct ttatcaatcg catcggcagt ggcaccaatc atggtgacac cgaactcttc
    56041 caacacgccc tgacgttcca gctccagcgc gcagttcagc gccgtctgac cgcccatggt
    56101 tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtaccactt cccagtgaat
    56161 cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg ccgggttgga
    56221 gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt gcgcgccaga
    56281 gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact
    56341 ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg
    56401 tactgctcaa ttaacttgat aaagtggtcg aacaacggcg cggcgtcgtg tggaccaggg
    56461 ctggcttcag ggtgaccctg gaagctgaac gccggcttat cgatgcgatg aatgccctgt
    56521 aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa tgttgcttca
    56581 tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc cacatcttta
    56641 accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc
    56701 agcgccagca gctgatgacc gagacagatg ccgaataccg gaatatcggt ttcgaggaac
    56761 ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg
    56821 aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg
    56881 gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca
    56941 taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag gccgccggtc
    57001 aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc ttttgccaga
    57061 tccatgccgt tcagacctgg gaacgcgcgg gctttttcta acgccagcgc cgcatccggg
    57121 ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc
    57181 ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta agaagagagg
    57241 tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgt
    57301 gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga
    57361 taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg
    57421 gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc tatggcccga
    57481 ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacaccctcc
    57541 agagaatact cactcacttt atttgcatat taattcacag tgatgacgta aatcaatgca
    57601 aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg ggcgctaaaa
    57661 gtcaacttaa tggcaggatt ttttatatta ttttgtggct catgcatgtt ttacaggcat
    57721 taagcgacaa aaacatcatc taacctgttc tggaaaacgc ttgcgcagca agaagattgc
    57781 atgaaataaa aacgagcagg aaaaagtgga ccaaatggtc aaaatttaca ttaaaacaac
    57841 aaaaatagaa gacgaaacat gtaatttttg gcagttatag aaaatttaaa agcataacac
    57901 caaaaataaa agggctaaaa attaaagccc tttataatta ataaattaaa tgtgttactt
    57961 ttgcatcata ataaacattt tgtggttaca aattattgag atcaagcaca tctcgcatat
    58021 caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgca ccgttagcaa
    58081 atgtcatgcg gctggacgcc ttatgggtga tctccagacg ctcgccaata tcggcaaaca
    58141 tcgcggtatg ttcgccaacg atgtcacctg cacgcacggt agcaaaacca atggtacctg
    58201 gcacacgttc accggtatgg ccttcacgac tgtagaccgc gcaatctttc agatctttat
    58261 caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc gcatcaactt
    58321 tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc actttggctg
    58381 ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg gcaaagacaa
    58441 tcgcaatatc ggcggcggcg tcacgaattg cctgtttacc ggcttcgtca aaccccgtag
    58501 tgccgatcac catccctttg ccatgctgcc gacaaaaagc gagatggttc agcgtacctt
    58561 ccggacgggt aaaatcgata aacacatcaa aatcatcttt tatcgcatca agactgcttt
    58621 gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg cccagtaaag
    58681 aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc agtgccgcct
    58741 gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg atgtttgcat
    58801 catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta accagcgcat
    58861 cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca gggagggata
    58921 tcagtgaaag gcatgagtcg atgaatgact gcatgccgat aacatgtgac aggttacggc
    58981 accagagcca gcacctcagc cacccactgt tggaagcctt ttacattcag atccaatgcc
    59041 acctgtacat tggctggttt gcccaggcaa ccgtcgatat caaccaccgt cgtgcctgag
    59101 gtaaattcgc cttgagtttc taccgcgaca aaacacggct tgagggtgaa taagtccggg
    59161 cgcaccagcc aggcgatggc gcagagatcg tgcattcgca gaccgctttg catactgccg
    59221 ctacggtagt ggctaaacag ggcgtggagc atttttccgg tgcgattcaa ctccggtaaa
    59281 gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc gcacatgaca
    59341 atttcgatgc cgctacggaa gacacaggca gccgcttctg gatcggcagc gatattaaac
    59401 tcggcatttg gtgtacagtt gccgcgacca gcagaaccgc ccatgatcac cagacgacga
    59461 atatacggtt tacattccgg gcattgtaaa agtaacagcg caatattggt taacgggccg
    59521 atggccacca gggtaacggg ctctggtgca cgcatcaggg catcccgaat cgccagaaac
    59581 gccggtatcc cgagtggctt tcggttgtgc tcaacaaagt cgtagccagc cattcccgat
    59641 tcgccgtgca cagatgctgc atcacgcggt gcgcgtacca gcggcacagc ggctccctgg
    59701 gcgagcggaa tctccgcatt ccagaaatgc agcagttgca gggcattgcg ggtagttttc
    59761 tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc gggggcaaaa
    59821 atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag gaagataggt
    59881 aaacgcatgt tttcttcata aaaaatgccg gtaacaagac cggcattttc gcataactta
    59941 ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc ggcacttcga
    60001 aaacaatatt ttcttcacgg ccttccagcg gaatggcttc accaccaccc aactgctgca
    60061 aacgtgccac cacattctgc accagaatat ccggagccga tgcgcccgca gtcacgccga
    60121 cgcatttaac ctctttcacc cactcttcct ggatatcttt cgcatcgtca atcaaaaacg
    60181 cgtgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag gagtttttcg
    60241 aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct tcctgacggt
    60301 tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg aagcgtttac
    60361 gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtttgg gtcataaagg
    60421 agagcttctc ttcgtttttg accgtcagtt tccacacatc gtctggcgat tcgactagat
    60481 acattccccc ttcggggttg ctgtactgcc ccatcgtccc ttccacttcc gggtgcccgg
    60541 cgtggccgat gagaatagat tcttcgccac ggcgactggc gcgggcaact tccatatgca
    60601 ctttggtcac cagcggacag gtggcatcaa acaccgtcaa atcgcggctt ttcgcttcgt
    60661 tacgaaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg tccggcactt
    60721 cgctaatctg ctcaataaag atagccccac gctcgcgcaa gctatcgacc acgtagcggt
    60781 tatgcaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc gcgttttcaa
    60841 caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc aacaggatct
    60901 gcatgttacg cctccagtgc cggatcgatt tccagcactt caatatcaaa atgaacggtc
    60961 tgcccggcca gcggatggtt gaaatcaacg gtaatggagt cgccgttaat ttcgcggatc
    61021 acaccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc aatttctggc
    61081 tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg tgacggcacg
    61141 ccaaacgccg catctggctc cagcgagaag gtggttttgt cgcccacttt cagccccagc
    61201 aggtgttgct ccagcccttc agaaagagaa gcatcgccca ggcggaacag cgccggttta
    61261 ccgttgttgc gagtagactc ggcggtggtg ccatcgtcga gttttagcgt gaagtgcacc
    61321 aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta tttttgagga
    61381 tttttgccgg atgcggccta cgatttcgta gcaatctgta ggccgcatcc ggcaggattt
    61441 attattgttt tttcgctttg gaaggtaaaa acccttccag cacgatcagt gccgcaccga
    61501 cacaaatggc agtatcggca aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt
    61561 cgatcatatc gacaacgaag ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc
    61621 caataatcag cgcgtaagcg atattgttta gcttctgcgt ggccttcgag cgatacatca
    61681 tcactgccag gagcacgcta ataccaatcg caataccggc aaagaaccaa cgctgccagc
    61741 cgccgctatc ggcaaggaaa ctaaacgccg cgccatagtt acgcgcataa tgcagattaa
    61801 gcgacgggaa cagcgggacc gtatccccca gagcaaagtt ctggaggatc aggtatttgc
    61861 tgcccagatc gataatcagc acgactacca ccagccacag ccagcgtagc cctgttgaac
    61921 agatcgattg actcatcagg caaacttacg tttttcaccg tcaccggcga cgttgctgac
    61981 acagcggccg cagatttctg cgtgttccgc caccttgccg acatcctggg tgtagtgcca
    62041 gcagcgtggg cacttctcac cttcggcttt actcaacgcg actttcagcc ctttgagtac
    62101 ttcgctctgc tgagcatcag caggtgcgtc gttatagtct gcaacggtag cgccggaggt
    62161 caacaggaca aatcgtaatt catcgcccag cgcggtcagt ttcgccgcca gttccggttc
    62221 tgcatacaag gttactgccg cttccagcga gccacccact ttcttgtcgg cacgcgcttg
    62281 ctcaatgact ttgttcactt cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt
    62341 cattgcttca ctgtctgcca gaccaaacag gccttcgtac cactcaccgg tgaagacgta
    62401 tttttcacgt tcgcccggca gatagcccca cacttcatca gcggtgaagg agaggattgg
    62461 tgccatccag cgcaccagcg cttcagcgat gtgatacagc gcagtctggc agctacgacg
    62521 cgccacgcta tccgctttgg cggtgtactg acggtctttg atgatgtcga ggtagaagga
    62581 acccatctca acggagcaga agcgcatcag acgctgtacc acttcgtgga aatcgtatgc
    62641 ttcgtacgcc ttgaggatgt cttcctgtgc cgctttcgca caacctacgg cccagcgatc
    62701 cagtaccacc atctcttccg gtttcaccat atcttttgct ggatcaaaac cattcaggtt
    62761 tgccagcagg aagcgcgcgg tgttacggat acgacgatag ctatcggcag cacgtttcag
    62821 gatctcgtca gaaacggcca tttcaccggt gtagtcggtt gatgccaccc acagacgcag
    62881 aatatccgcg cccagtttgt tcatcacatc ctgcggcgaa acggtgttgc cgatggattt
    62941 agacatcttg cgaccctgac catccacggt aaagccgtgg gtcagtacct gacgatacgg
    63001 cgctttgcct ttcatcgcgg tggagatcat tagggaagac atgaaccaac cacggtgctg
    63061 gtcagaacct tccagataca tgtccgctgc gtgaccggca aattccggac gcacgtcaac
    63121 aacagaagag tgggtagatc cggagtcaaa ccatacgtcg agagtatccg gcactttcac
    63181 atactggtca gcttcgtcgc cgaggatctc tttcgcatcg agatcccacc acgcctggat
    63241 gccgtcaact tcaacgcgtt tagccacttc ttccatcagt tcgagggtac gcggatgcag
    63301 ctcttccgtg tctttgtgca cgaacagtga catcggtacg ccccaggtgc gctgacggga
    63361 gatacaccag tcaggacggt tagcaaccat cgactcgata cgcgcctggc cccagtccgg
    63421 gagccactgc acgcctttga tctctttcag tgactgcgca cgcagacctt tctgatccat
    63481 gctgacgaac cactgcggtg tcgcgcggaa gatgatcggc gttttgtgac gccagcagca
    63541 cggatagctg tgctgcattt tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc
    63601 aacgacgatg tcgttcgctt tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg
    63661 cagataagtg ccgtccgggc caaccgggtt agcggtttcc aggccgtatt tctgaccgat
    63721 cacatagtcg tccgggccgt ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag
    63781 ggtaacgtga tcgccgagga ttgccggaac gtcgaagccc ataaacggat gggtaaagcg
    63841 cagcagctca agctctgcac cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat
    63901 acgctgcatt acgctttcaa ccagatcttt cgccagaatc acggcctgac cgtcgatctg
    63961 caccagcgca tagtcgaaat caggtgcaat agagattgcg cggttcgcag gcagagtcca
    64021 cggcgtagtg gtccagatca ccagcgagat tgggccgtta acgttgctta cgccaaattt
    64081 cgctttcagc gcatcctgat cgaccgcctg gaaagcgacg tcaatggacg gagaagtttt
    64141 gtcgtaatac tcaacttccg cttctgccag tgcagaacgg cagtctacgc accagtgcac
    64201 cggcttcgcg cctttgtgca ggtgaccatt gccgatgatt ttgcccagcg cgcggatgat
    64261 gttggcttca gttttgaagt ccatagtcag atacgggtgc gaccagtcgc ccagcacgcc
    64321 cagacggata aagtctttgc gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt
    64381 ggcgcggaac tcggcggcgg tgaatttctc acccggctta ccgtattctt gctctacttt
    64441 cagctcgatc ggcagaccgt ggcagtccca gccaggcaca tacggcgagt cataaccgga
    64501 aagccctttg gacttcacga taatgtcttt cagaatcttg ttaaccgagt gaccaatatg
    64561 aatgctgcca ttcgcataag gagggccatc atgcagaatg aaggtttttt tgcctttttt
    64621 agccgcacgg atgatgccgt acagatcatc atcagtccaa cgcgccagca ttcccggttc
    64681 gcgcttggcg agatcgccac gcatcgggaa ccctgtttcc ggcaaattca gggttgattt
    64741 atagtcactc atcagattct cggttccgtg tttcggtttg attacataac aggcttaagc
    64801 cggttttgtt agcccaaaaa attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt
    64861 cagttcgtcc agcgacgcaa atcgctgctc attgcgtatt tttttacgca gcactacttg
    64921 tatatggcga ccgtaaaggt ccattgcaac atctaacaaa tgcacttcca gctgctggcg
    64981 aataccggca accgttgggc gtgttccgat gtttgccacg ccgggtaacg gcttttcacc
    65041 gaggcccagc acttctaccg cataaacccc tttcaccggg gaaacctgac ggcgtagcgg
    65101 tacattcgcc gtcgggaaac ctatagtgcg ccctaattca tcaccgtgga ctacacgccc
    65161 ggagatggca aacgggtgcc ccagtaaact ttctgccaga gccagattgt catcagccag
    65221 cgcctgacgc acggcggtgc tgctgatacg cacaccacct tcgcaaaaag tttgcgtact
    65281 ggtgatatcg aagccgtatt ccatgccagc tttctgtaat aacaagaaat cgccttcacg
    65341 accagcgcca aagcggaaat catcacctac ggcaagaaat tttacgcgca aatgcttcac
    65401 caaaagatcg ctgatgaaat tttgcgcggt taacgccgca aaacgcctgt cgaaacgcac
    65461 gcacagcacg taatcaacgc cacactctgc aaggtaacgc agtttttccc gcagccgggt
    65521 cagtcttgcc ggggctttat cggtagcaaa cagttccagt ggttgaggtt caaaaagcat
    65581 caccatcacc ggtaagttgc gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg
    65641 atgaccgcga tgcacgccgt cgaaattacc aatagtcagc acacaccctt cttgcggggc
    65701 ctggctgaga ttatgtatgc cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc
    65761 gtccgagtat accttgtaca gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa
    65821 agaagtggct tttcattgcc atggagcaaa tcacgggaag aaactgaccg cctgctgcaa
    65881 tttttatcgc ggaaaagctg tattcacacc ccgcaagctg gtagaatcct gcgccatcac
    65941 tacgtaacga gtgccggcac attaacggcg cttatttgca caaatccatt gacaaaagaa
    66001 ggctaaaagg gcatattcct cggcctttga attgtccata tagaacacat ttgggagttg
    66061 gaccttggct aatatcaaat cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa
    66121 gcacaacgca agccgtcgct ctatgatgcg tactttcatc aagaaagtat acgcagctat
    66181 cgaagctggc gacaaagctg ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga
    66241 ccgtcaggct gctaaaggtc tgatccacaa aaacaaagct gcacgtcata aggctaacct
    66301 gactgcacag atcaacaaac tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc
    66361 cgcgcaagcg ggttttttta tgcctgctgc ttttgtgatg cgtgagagaa cacactgccg
    66421 gttgccagat agtcaaaaga gacatgccgt tgtactatct gaactcttcg tggcttaaga
    66481 atcgtagaac catcggctga tacattctga acaagaatat ctactcaatc tccagactcc
    66541 cccacaaaga atatggatat tgtgatacac attgaggtat taaacttaag accactctat
    66601 ttttgaatta aattcctata tactttaacc attcataaat atctcctcat tagacct
//
LOCUS       DAJTRM010000008        66345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRM010000008 DAJTRM010000000
VERSION     DAJTRM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.008"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..2280)
                     /gene="glgP"
                     /locus_tag="OO322_000577"
     CDS             complement(<1..2280)
                     /gene="glgP"
                     /locus_tag="OO322_000577"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5905576.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLAGY"
     gene            complement(2299..3732)
                     /gene="glgA"
                     /locus_tag="OO322_000578"
     CDS             complement(2299..3732)
                     /gene="glgA"
                     /locus_tag="OO322_000578"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5905577.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3732..5027)
                     /gene="glgC"
                     /locus_tag="OO322_000579"
     CDS             complement(3732..5027)
                     /gene="glgC"
                     /locus_tag="OO322_000579"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5905578.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5045..7018)
                     /gene="glgX"
                     /locus_tag="OO322_000580"
     CDS             complement(5045..7018)
                     /gene="glgX"
                     /locus_tag="OO322_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5905579.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7015..9201)
                     /gene="glgB"
                     /locus_tag="OO322_000581"
     CDS             complement(7015..9201)
                     /gene="glgB"
                     /locus_tag="OO322_000581"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5905580.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9474..10577)
                     /gene="asd"
                     /locus_tag="OO322_000582"
     CDS             complement(9474..10577)
                     /gene="asd"
                     /locus_tag="OO322_000582"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5905581.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10769..11362
                     /gene="yhgN"
                     /locus_tag="OO322_000583"
     CDS             10769..11362
                     /gene="yhgN"
                     /locus_tag="OO322_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5905582.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11419..12759)
                     /gene="gntU"
                     /locus_tag="OO322_000584"
     CDS             complement(11419..12759)
                     /gene="gntU"
                     /locus_tag="OO322_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5905583.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12763..13290)
                     /gene="gntK"
                     /locus_tag="OO322_000585"
     CDS             complement(12763..13290)
                     /gene="gntK"
                     /locus_tag="OO322_000585"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5905584.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13429..14424)
                     /gene="gntR"
                     /locus_tag="OO322_000586"
     CDS             complement(13429..14424)
                     /gene="gntR"
                     /locus_tag="OO322_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5905585.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14648..15343)
                     /gene="yhhW"
                     /locus_tag="OO322_000587"
     CDS             complement(14648..15343)
                     /gene="yhhW"
                     /locus_tag="OO322_000587"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5905586.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15466..16503)
                     /locus_tag="OO322_000588"
     CDS             complement(15466..16503)
                     /locus_tag="OO322_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5905587.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16836..17324
                     /gene="yhhY"
                     /locus_tag="OO322_000589"
     CDS             16836..17324
                     /gene="yhhY"
                     /locus_tag="OO322_000589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5905588.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17383..19125)
                     /gene="ggt"
                     /locus_tag="OO322_000590"
     CDS             complement(17383..19125)
                     /gene="ggt"
                     /locus_tag="OO322_000590"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5905589.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19245..19685
                     /locus_tag="OO322_000591"
     CDS             19245..19685
                     /locus_tag="OO322_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5905590.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19672..20415)
                     /gene="ugpQ"
                     /locus_tag="OO322_000592"
     CDS             complement(19672..20415)
                     /gene="ugpQ"
                     /locus_tag="OO322_000592"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5905591.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20412..21402)
                     /locus_tag="OO322_000593"
                     /pseudo
     CDS             complement(20412..21402)
                     /locus_tag="OO322_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21404..22249)
                     /gene="ugpE"
                     /locus_tag="OO322_000594"
     CDS             complement(21404..22249)
                     /gene="ugpE"
                     /locus_tag="OO322_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5905592.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22246..23133)
                     /gene="ugpA"
                     /locus_tag="OO322_000595"
     CDS             complement(22246..23133)
                     /gene="ugpA"
                     /locus_tag="OO322_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5905593.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23231..24547)
                     /gene="ugpB"
                     /locus_tag="OO322_000596"
     CDS             complement(23231..24547)
                     /gene="ugpB"
                     /locus_tag="OO322_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5905594.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(24944..25657)
                     /gene="livF"
                     /locus_tag="OO322_000597"
     CDS             complement(24944..25657)
                     /gene="livF"
                     /locus_tag="OO322_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5905595.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25659..26426)
                     /gene="livG"
                     /locus_tag="OO322_000598"
     CDS             complement(25659..26426)
                     /gene="livG"
                     /locus_tag="OO322_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5905596.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26423..27700)
                     /gene="livM"
                     /locus_tag="OO322_000599"
     CDS             complement(26423..27700)
                     /gene="livM"
                     /locus_tag="OO322_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5905597.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27697..28623)
                     /gene="livH"
                     /locus_tag="OO322_000600"
     CDS             complement(27697..28623)
                     /gene="livH"
                     /locus_tag="OO322_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5905598.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28671..29780)
                     /gene="livK"
                     /locus_tag="OO322_000601"
     CDS             complement(28671..29780)
                     /gene="livK"
                     /locus_tag="OO322_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5905599.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30204..30587
                     /gene="panM"
                     /locus_tag="OO322_000602"
     CDS             30204..30587
                     /gene="panM"
                     /locus_tag="OO322_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5905600.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30786..31889)
                     /gene="livJ"
                     /locus_tag="OO322_000603"
     CDS             complement(30786..31889)
                     /gene="livJ"
                     /locus_tag="OO322_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5905601.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32160..33014)
                     /gene="rpoH"
                     /locus_tag="OO322_000604"
     CDS             complement(32160..33014)
                     /gene="rpoH"
                     /locus_tag="OO322_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5905602.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33259..34317)
                     /gene="ftsX"
                     /locus_tag="OO322_000605"
     CDS             complement(33259..34317)
                     /gene="ftsX"
                     /locus_tag="OO322_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5905603.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34310..34978)
                     /gene="ftsE"
                     /locus_tag="OO322_000606"
     CDS             complement(34310..34978)
                     /gene="ftsE"
                     /locus_tag="OO322_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5905604.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(34981..36477)
                     /gene="ftsY"
                     /locus_tag="OO322_000607"
     CDS             complement(34981..36477)
                     /gene="ftsY"
                     /locus_tag="OO322_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5905605.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36627..37223
                     /gene="rsmD"
                     /locus_tag="OO322_000608"
     CDS             36627..37223
                     /gene="rsmD"
                     /locus_tag="OO322_000608"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5905606.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37213..37482
                     /locus_tag="OO322_000609"
     CDS             37213..37482
                     /locus_tag="OO322_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5905607.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37485..37844)
                     /locus_tag="OO322_000610"
     CDS             complement(37485..37844)
                     /locus_tag="OO322_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5905608.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            37985..38611
                     /locus_tag="OO322_000611"
     CDS             37985..38611
                     /locus_tag="OO322_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5905609.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38685..40883
                     /gene="zntA"
                     /locus_tag="OO322_000612"
     CDS             38685..40883
                     /gene="zntA"
                     /locus_tag="OO322_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5905610.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(40985..41230)
                     /gene="tusA"
                     /locus_tag="OO322_000613"
     CDS             complement(40985..41230)
                     /gene="tusA"
                     /locus_tag="OO322_000613"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5905611.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41451..42116
                     /locus_tag="OO322_000614"
     CDS             41451..42116
                     /locus_tag="OO322_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5905612.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42189..42746
                     /gene="dcrB"
                     /locus_tag="OO322_000615"
     CDS             42189..42746
                     /gene="dcrB"
                     /locus_tag="OO322_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5905613.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42750..43967)
                     /locus_tag="OO322_000616"
     CDS             complement(42750..43967)
                     /locus_tag="OO322_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5905614.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44101..45150
                     /locus_tag="OO322_000617"
     CDS             44101..45150
                     /locus_tag="OO322_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5905615.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45205..45792
                     /gene="acpT"
                     /locus_tag="OO322_000618"
     CDS             45205..45792
                     /gene="acpT"
                     /locus_tag="OO322_000618"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5905616.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            45903..47477
                     /gene="nikA"
                     /locus_tag="OO322_000619"
     CDS             45903..47477
                     /gene="nikA"
                     /locus_tag="OO322_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5905617.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47477..48421
                     /gene="nikB"
                     /locus_tag="OO322_000620"
     CDS             47477..48421
                     /gene="nikB"
                     /locus_tag="OO322_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5905618.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48418..49251
                     /gene="nikC"
                     /locus_tag="OO322_000621"
     CDS             48418..49251
                     /gene="nikC"
                     /locus_tag="OO322_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5905619.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49251..50015
                     /gene="nikD"
                     /locus_tag="OO322_000622"
     CDS             49251..50015
                     /gene="nikD"
                     /locus_tag="OO322_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5905620.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50012..50818
                     /gene="nikE"
                     /locus_tag="OO322_000623"
     CDS             50012..50818
                     /gene="nikE"
                     /locus_tag="OO322_000623"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5905621.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50824..51225
                     /gene="nikR"
                     /locus_tag="OO322_000624"
     CDS             50824..51225
                     /gene="nikR"
                     /locus_tag="OO322_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5905622.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51345..51704)
                     /locus_tag="OO322_000625"
     CDS             complement(51345..51704)
                     /locus_tag="OO322_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5905623.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51701..51976)
                     /locus_tag="OO322_000626"
     CDS             complement(51701..51976)
                     /locus_tag="OO322_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5905624.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52035..53159)
                     /locus_tag="OO322_000627"
     CDS             complement(52035..53159)
                     /locus_tag="OO322_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5905625.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53159..55894)
                     /gene="rbbA"
                     /locus_tag="OO322_000628"
     CDS             complement(53159..55894)
                     /gene="rbbA"
                     /locus_tag="OO322_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5905626.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55891..56958)
                     /locus_tag="OO322_000629"
     CDS             complement(55891..56958)
                     /locus_tag="OO322_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5905627.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57324..58946)
                     /locus_tag="OO322_000630"
     CDS             complement(57324..58946)
                     /locus_tag="OO322_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5905628.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59208..60797)
                     /locus_tag="OO322_000631"
     CDS             complement(59208..60797)
                     /locus_tag="OO322_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5905629.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61182..62213
                     /locus_tag="OO322_000632"
                     /pseudo
     CDS             61182..62213
                     /locus_tag="OO322_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62300..62776)
                     /locus_tag="OO322_000633"
     CDS             complement(62300..62776)
                     /locus_tag="OO322_000633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905630.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62769..63533)
                     /locus_tag="OO322_000634"
     CDS             complement(62769..63533)
                     /locus_tag="OO322_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5905631.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63779..64540
                     /locus_tag="OO322_000635"
                     /pseudo
     CDS             63779..64540
                     /locus_tag="OO322_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64647..65147)
                     /locus_tag="OO322_000636"
     CDS             complement(64647..65147)
                     /locus_tag="OO322_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905632.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65298..>66345)
                     /locus_tag="OO322_000637"
     CDS             complement(65298..>66345)
                     /locus_tag="OO322_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5905633.1"
                     /translation="DGSTISLSQPLGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVW
                     RGITFSINWAKNDLLTSHKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNY
                     QVSLSGQNSGGVWWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQ
                     LGANLSGGILITRDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAIQPVQ
                     PYRRDDVILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKP
                     IPFGAVISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAK
                     KSASGLYQIAATCL"
BASE COUNT        16125 a        17817 c        16284 g        16119 t
ORIGIN      
        1 ataacccgcc agtacctggt agtgatcgcc gaagttgatc agcgaatcaa ccagatcgcg
       61 atagcgaccc ggatcttccg gactgaatac accgctgccg atttgcgtca gcacctgatg
      121 cagctcctca tctttctcgt agtattcacg cggtttgtag ccctgacgac gcagttcttc
      181 cacttcttcc gctgtgttac caaaaataaa gatattgtca gcaccgacat gatccagcat
      241 ctcgacattc gcaccgtcca acgtaccgat agtcagcgca ccgttaagcg caaacttcat
      301 gttactggtg ccggaagctt ccgtccctgc cagcgaaatc tgttcagaca gatctgccgc
      361 cggaatgatc aactgcgcca ggctaacgct gtagttcggg atgaacacga ctttcagctt
      421 atcgccaatc tgcggatcgt tgttgatcac tttcgctacg tcattgatca aatgaataat
      481 gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca aaaatattca cgcgcggtac
      541 ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg gtaatcacat gcaacacatt
      601 catcaattga cgtttgtatt cgtgaatacg tttgatttgt acatcgaaca acgcctttgg
      661 attcaccacc acattcagct gctgggcgat atactctgcc agacgctttt tgttctccag
      721 cttcgcctga tgcacagcgt gattaaccat tgggaaatca cagtgttgtt gcagctcatt
      781 aagcaggcta aggtcggtgc gccagttacg gcccaggtgt tcgtccagca cggctgaaag
      841 cgatgggttc gctaccgcca gccagcgacg cggcgtcaca ccgttggtga cgttggtgaa
      901 acgacccggg aagattttcg caaagtcggc aaacaacgat tgcaccatca gattagagtg
      961 cagttccgat acaccgttaa ctttgtggct cacaacaacc gccagccagg ccatacgcac
     1021 acgacgaccg ttggattcat caatgatcga cgcccgtccc agcagatcgg tatcgttcgg
     1081 atactgttcc tgcaaggttt tcaggaaata gtcgttgatt tcaaagatga tctgcaggtg
     1141 acgcggcaga attttaccca gcatatcaac cggccaggtt tccagcgcct cgctcatcag
     1201 cgtgtggtta gtgtaggaga agacctgaca acacacctca aacgcgtcgt cccagctaaa
     1261 ttggtgctca tcgatcagca gacgcatcat ctcaggaatc gacagtaccg gatgggtatc
     1321 attgagatga atcgcgattt tatccgccag gttatcgtag gttttatgca actgataatg
     1381 acggcttaaa atgtcctgaa tggtcgagga aaccaagaag tattcctgac gcaggcgcag
     1441 ctcacgcccg gagtaggtgg agtcatccgg atacagtacg cgagatacgt tctcggagtg
     1501 gtttttatct tccactgccg cgaagtagtc accctggttg aatttaccga ggttaatttc
     1561 gctactggct tgcgcactcc acaaacgcag cgtgttggtc gcgtcggtgt cgtaaccagg
     1621 gattatctga tcgtaagcga ctcccagaat ctcttcggtt tcaatccagc gcgttttttt
     1681 accttcctgc tgaatgcgac caccaaaacg gactttatag cgcgtgttgt ggcgtttgaa
     1741 ttcccacggg ttaccgtatt ccagccagta gtctggcgac tctttctggc taccgttaac
     1801 gatgttctgc ttgaacatac cgtagtcata gcggatgccg taaccgcgcc ccggcaaccc
     1861 taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt cccaggccac cgttaccgag
     1921 gcctgggtca ttttcttcat caatcagctc ttcgagattt aaccccatcg cttccagtgc
     1981 gccctgtaca tcttcgtaaa ttcctagcga caacatggcg ttggagagcg tacggccaat
     2041 caaaaactcc atcgacaggt agtaaacctg acgagtttct tgcgacaact gggcacggtt
     2101 tgaacgtaac cagcgctcca cgagacgatc gcgcacagca aataacgttg cgttcagcca
     2161 ttcatgttta ttggcgacga ccgggtcctt tccaatcgta aacatcagct tgtaagcgat
     2221 agagtgctta agagcttcta cgctaagcgt gggcgatgaa tatgtaaacg gagcattcat
     2281 ataggcgttt cctgaaaact atttcaagcg atagtaaagc tcacggtacg acttcgccgc
     2341 gacctgccag ctaaaatcca ttgccatagc ctgacgttgc acaaaacgcc acagtgaagg
     2401 acgggaccac agtacaaaag cacgccgaat agcccgtaac agcgaccagg cattactatc
     2461 ttcaaagaca aacccactgg cgacgccatc tgcaaggttc tcaagagaac agtcagaaac
     2521 cgtatcagca agcccaccgg tgcgccgcac taacggcagc gtaccgtact tcaatccata
     2581 aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc agaatgacgt ccgcgccgcc
     2641 cataatgcga tgcgaaaatg cttcgtgata gccaatctga acgcccacct gaccggggta
     2701 ttccgctgcc gccgcaagga aaccttcctg cagcaccgga tcgcccgcgc cgagtagcgc
     2761 cagctgcccg ccctgctcca gaagccccgg taaggcttcc agcaccaggt cgagaccttt
     2821 ctggctggtc agacggctca ccactgcaaa aagcggcact ttatcgtcaa ccttaagccc
     2881 cattgcgatt tgtaactggc tcttattttc cgctttatct tccaacgtat cgcgggtgta
     2941 acgcgaggcc aacagtaagt ccgtctctgg actccagatt ttctcgtcca cgccgttcag
     3001 tacgcctgaa agacgccctt cacggtgacg ctgttgcaac agaccttcca taccgtaggc
     3061 aaactgcggt tcggtgatct cgcgagcgta ggttggactg accgccgtaa tgtgatcggc
     3121 atagtacaga ccggccttca ggaaagagat ttgtccgttg aattccagcc catgaatatt
     3181 aaagaatgac catggcaatt ggatgtcatt catgtgatgt gcataaaaca tgccttgata
     3241 ggccaggttg tgcacagtaa acaccgactt cgcaggacgc ccgcgcgccg ccagatacgc
     3301 aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca tcaggacgcc agaatgggtc
     3361 aagcccgctg gccatttctg ccccaaccca ccccagcagc gcaaaacgca atacgttgtc
     3421 ggtataggca aataagttgg tatcgtgata cgggcttccc ggacgatcat agagatgcgg
     3481 cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg aacaacagcg tgatatgccc
     3541 ggcgaaagta tcacgacggg atactacctg cgcatcggtc acgccacggc gaatatcggg
     3601 aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg atttgtgctg cgggtaatgc
     3661 cccaataaca tcagccagac cgccggtttt aagcagcggg aacatctctg aacatacatg
     3721 taaaacctgc attatcgctc ctgtttatgc cctaacttcc gtagcatttc gcgcgttacc
     3781 agcacgatgc cttcttctga acgatagaaa cgacgtgcat cttcctctgc gttttcacca
     3841 atcaccatgc cttccgggat aacacaagca cgatcgatga cgcagcggcg cagacggcac
     3901 gagcgaccta cccatacttc cggtaacaat acggcggaat caatgttgca gaatgaattc
     3961 acgcgaacgc gcgagaacag aacggactgc accaccaccg aaccggagat cacacaaccg
     4021 ccggatacca gtgagttaag ggtcatcccg tggctaccgg agcgatcctg cacgaatttc
     4081 gctggcggta atgattcatt gtaggtgcga attggccaat tgcgatcgta catatccagt
     4141 tccggcacca cagaggccag atcgagattc gctttccagt aagcttccag cgtacccaca
     4201 tcgcgccagt acggctcggc atccgggtcg gattgtacgc aagagagcgg gaacgggtgc
     4261 gcataggcca gaccggcttc ggtgatcttg ggaatcaaat ctttgccaaa gtcgtggctg
     4321 gagttctcat cacgatcgtc ttcttccagc agttcataca gatagtcggc gtcaaagacg
     4381 tagataccca tactcgccag agatttgctc ggatcgttcg gcattgacgg tgggttagca
     4441 ggtttttcaa cgaattcgat aattttatcg ttctcatcaa ccgccataac gccaaatgcg
     4501 gaggcttctt caatcggtac tggcatacaa gcaacggtgc aacgtgcgcc tttttcgacg
     4561 tgatcgataa gcatacgcga gtagtcttgc ttgtagatat ggtcgcccgc caggatcacc
     4621 acgtattccg ctttataacg gcggataatg tcgaggtttt gggtgaccgc atctgcggtg
     4681 ccgcgatacc agttttcccc tttcattctc tgctgtgctg gcagcagatc gacaaactcg
     4741 ttcatttctt cattgaagaa tgaccagccg cgctgaatgt gctgcaccag agtgtgggac
     4801 tggtactggg tgatcacgcc catacgacgg atcccagagt tgatgcagtt agacagcgca
     4861 aagtcaataa tgcggaactt accgccgaag tgtacggccg gttttgctcg cttattggtt
     4921 aaatccttca ggcgggtacc acgtcctccc gccagtatca gggcaacaga tttcaatggc
     4981 agctggcgcg ccaacattaa gtgatcgttc ttctctaaac taaccatgac taactccttt
     5041 tttatcatct ctggaacaca cacaatccgt gtgcaggtcc ctgccagaca accgtaatca
     5101 ctgggttatc ctctccagcg aatgggggaa tggcgtgcca ctccccagca ggtaaaacaa
     5161 tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa gcgatccgag agcagaattt
     5221 gcagctgttt cggcccgttt tgccactcat ccgtgcttaa aggttgagca tatcgattta
     5281 gccaacggac attgccgtca ccttcttccc accagcgatt ctccaccaaa gcgggaatgc
     5341 gcttgcgcag atggattaac gcggcggtaa atgcggttaa accactgctt gcctgcgacc
     5401 agtccaacca gtttaattgg ttatcctgac agtaggcatt gttattgcca tgctggctgt
     5461 gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg ggagagcaac aacgttgtta
     5521 acagggcatg aatgctgtcg cgccgccgtt caaccaggtc aagagtaccg cctaaccctt
     5581 ctttaccatg attgttactg taattgttgt tggtcccgtc gcgattttct tctccgtttg
     5641 cttcattgtg tttatggttg aagcaaacgc agtcgcgaag cgtaaaaccg tcatgcgcgg
     5701 tgacgagatt aatcgcggca ctcggcagac gaccattacg tttaaaaaca tcgctggagg
     5761 cagcaaaacg cccggcaaac gcccccagag gcaaatcata atgtagccag aaacgacggg
     5821 cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg cgggaaattt cccacctgat
     5881 aaccactagg agcgatatcc cacggttcag caattaactt cacctgcgag agcaccgggc
     5941 agttctggat agcggtaaac aacggcgcat cctgacggaa ctctggcgta cggcccatga
     6001 ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt ttctacccaa taacgcaggc
     6061 aggcgctggc ataatccacc accgccggat gactcaaatt gagcgtgttg ccgcaaccgg
     6121 tccagttgtg ataatcgccg tcttctctta tccaataata gctacggtta tcgatcccac
     6181 gcagcgagaa taacgggccg tcgaggtcca gttccgcact atggttgagc acgatatcaa
     6241 gaatgacttc gatacccgct ttatgcagtg ctttgattgc atcgcgaaac tcatccagcg
     6301 ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa catcgccacc gggttgtaac
     6361 cccagtagtt acttagcccc atgcgttgca gacgtggttc actggcaaac tgcgccactg
     6421 gcagcagttc cagcgcggta atgcccaatt gtttcaaata gttgatcatc accggatgcc
     6481 cgagggcttt ataagtgcca cggatctcga ccgggatctc cgggtgcaag tacgttaatc
     6541 ctttgacatg ggcttcataa atgatggtgc tgccccacgg cgtgcgcggc ggggcgtcat
     6601 cttcccagtc atagtgatca accaccacta cgcatttcgg cgcaatggcg gcgttgtcgc
     6661 gatagtcagg ttcattatga ccggcgtgca gcagcgggtt atctttaaac tccccgtcaa
     6721 tttgccgcgc gcaaggatca atcaacaact tcgccgggtt aaagcgatgc ccctcggcgg
     6781 gttgccaggg gccatgaacg cgataaccat aacgcaaacc cgggcgcgca tccggcagat
     6841 aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata gcgatgttcc tggccattgg
     6901 cgtcaaagac acacagttct acccgctcgg catgagcgga gaaaagtgtg aagttgacgc
     6961 cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc aatggcgagt tgtgtcattc
     7021 tgcctcccga accagccaga tagtggccag cggtggtagc gtcaggctta gtgaatgctg
     7081 acgaccgtgg ctggcaatct catcgctgtg taccgtgccg ccattgcctg cattactgcc
     7141 gtgatagtgt atggaatcgg tattgaggat ttcacgccat ttacctggct gattaatgcc
     7201 gaagcgataa tcatgacgcg gcaccggcgt aaagttactg gcaacgataa tttcgttacc
     7261 ctctttatcg cgacgcacaa agatcagcac cgagcgttct ttgtcatcca ccaccagcca
     7321 ttcaaagccg tacgggtcaa aatccagttc atgcattgct ttatggtggc ggtaggtgag
     7381 gttcagatcg cgcaccagac gctggacacc gtggtgccag ttatcgccgc cttccaacag
     7441 atgccagtcg aggctggcgt catggttcca ctcgcggccc tgggcaaatt cgttccccat
     7501 gaacagcagt ttcttgcccg gaaatgccca catccagcca tagtaagcgc gcaggttagc
     7561 gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc gattttttac cgtggaccac
     7621 ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag ttgtagagca tcccgaaggt
     7681 cagtttatcg tgatgatact gacgataaat cgggtcgagc ttcatgtagt ccagggtgtc
     7741 atgcatccag ccgaggttcc acttgtacca gaagcccaga ccgcccatat cctgcggacg
     7801 agaaacgcca gggaaatcgg tagactcctc agccattgtc accgcaccgg aaacctgctc
     7861 accaagaata cggttggtat tacgcaagaa ttcaatcgct tcaagattct cgcgcccgcc
     7921 aaattcgttc gggatccact ccccctcttt acggctgtag tcacgataaa tcatcgacgc
     7981 caccgcatcg acgcgcagcg catcaatacc aaaacgttca atccagtaaa gcgcgttacc
     8041 gacgaggaag ttactgactt cacggcgacc atagttgtag atcagcgtgt tccagtcctg
     8101 atgatagcct tcacgcggat cgctgtgttc atacaagttc gtgccatcaa attcggcaag
     8161 cgcaaagtca tcagtcggga agtggcctgg cacccagtcg agaatcacgt tcagaccagc
     8221 tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga gtaccaaaac ggcgggttgg
     8281 tgcatacagg ccggttggct gataacccca actgccatcg aagggatgct cgttaatggg
     8341 cagtagttcg aggtgggtaa agcccatcca tttagcataa ggcaccagtt gatcggccag
     8401 ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga cgccaggaac ccaggtgaac
     8461 ttcataaata gagattggcg catcaaactg attcgctttt ttgcgctctt cagtctgtac
     8521 aaccttttcc ggcagcccgc aaataagaga cgcggtttcc gggcgcattt gcgcttcgaa
     8581 ggcataaggg tcggacttca gacgcaagtt gccattggca tcaatcatct cgtatttata
     8641 gagctgaccg ttatgcgccc cagggataaa cagttcccag atgccgctct ctttacgcag
     8701 gcgcatcggg tgacggcgac cgtcccagta gttgaattgc ccaaccaccg agacccgacg
     8761 ggcgtttgga gcccagacag agaaacgcgt acctgtgacg ccatccatag tatctgcatg
     8821 cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca gataatagcc aggcatccat
     8881 ttcctggatt agcggaccaa aacggtaagg atcatcaatc aggttttgct gaccatgcca
     8941 gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc ggaatgacgc cgctaaagaa
     9001 tccccgtgag tcgagacact ccagttttgc gagtttgcgc ccggttttcg gttcaatcac
     9061 ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc agtcccgcgg tggttttatg
     9121 cattcccagt acggaaaaag gatccgcaaa atggcctgca attagcgcgt taatcacgtc
     9181 tctatcgata cgatcggaca tgcttgtctt cctgttttat tgtgtcaccc catccaattg
     9241 gatttttgac ttctggttgt gacatttttt tgacctgaac ggcgcagcac tctgtgcatc
     9301 ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac tcatgtattc agccaccctt
     9361 aaagaatagc caatgctcta tttaactccc ggtaaatcat gaaacatctg cgcttactcc
     9421 tgtattacgc actaacaggg gcggcatcgc gccccagatt taatgaataa agattacgcc
     9481 agttgacgaa gcatccgacg cagcggctcc gcggcccccc acagcagctg gtcgcccacg
     9541 gtaaaggctg acaggaactc tggtcccata ttcagcttac gcaggcggcc taccggcgtg
     9601 gtcagcgtgc cggtaacggc agctggggtt agctcacgca tagtgatttc ccgatcgttc
     9661 ggaacgactt tcgcccacgg attgtgcgca gccagcagtt cttcgacggt cggaatagac
     9721 acatcttttt tcaatttaat agtgaatgcc tggctgtggc agcgcaatgc cccgacacgc
     9781 acacataaac catctaccgg aattacggaa gatgtgttga ggatcttgtt ggtttccgcc
     9841 tgccctttcc actcttcgcg gctctgaccg ttatcgagct gtttgtcgat ccacggaatc
     9901 aggctacccg ccagcggcac gccaaagtta tccaccggca gctcaccgct acgggttaag
     9961 gttgtgactt tgcgttcgat atcgagaata gcagaggacg gggtcgcgag ttcatctgcc
    10021 acatggccat acagatggcc catctgggtt aataactcac gcatatgtcg cgcaccaccg
    10081 ccggaagcgg cctggtaggt tgcaacggac acccaatcaa caagatcatt ggcgaataaa
    10141 ccacccaacg acatcaacat caggcttacg gtacagttac cgccaacaaa agtcctgatg
    10201 ccattattta atccgtcggt aatgacgtcc tgattgacgg ggtcaagaat gatgatggcg
    10261 tcatctttca tgcgcagaga cgatgctgcg tcaatccagt aaccttgcca tccgctttca
    10321 cgaagctttg gatagatttc gttggtataa tcgccgccct gacaggtcac aatgatatcg
    10381 agggccttta gcgcctctag atcaaaggca tcctgaagtg tgccagtggt tccgccaaaa
    10441 gacggcgcag cctggccaag ctgagaagta gaaaagaaga cagggcgaat ggcgtcgaag
    10501 tcgcgctctt caaccatgcg ttgcatgaga acggagccga ccataccgcg ccagccgata
    10561 aaaccaacat ttttcataag cgttttttcc tgcaaagatg tgtgctgtat aaatgtgccg
    10621 gtctcctctt ggcacatctt tcaccataca aaaagctgcc aaagtcgcaa gtgaaattaa
    10681 tcaatgatag cgaagccatc agtaatgcga cttatcctgc tttgttagca cgcagaaagt
    10741 ccgcggcaat tatcagggaa tttgagttat gaatgaaatc atttctgcag cagttttatt
    10801 gatcctgatt atggatccgc tcggaaacct gcctattttc atgtccgtac tgaaacatac
    10861 tgaaccgaaa agacggcggg caatcatggt gcgagagttg cttattgctc tcctggtgat
    10921 gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt ctcagcctgc gagcagaaac
    10981 cgtctccatt tctggcggca tcattctgtt tctaatcgcc attaagatga ttttccccag
    11041 cgcttcagga aatagcagcg ggcttccggc aggtgaagag ccatttatcg tgccgttggc
    11101 aattccgtta gtcgccgggc cgactattct cgccacgctg atgttgttgt ctcatcagta
    11161 cccgaatcag atgggacatc tggtgattgc tctgctgctg gcctggggcg gcacctttgt
    11221 catcctgcta cagtcttcgc tatttttacg tctgctgggc gagaaagggg tgaacgcact
    11281 tgaacgcctg atgggattga ttctggtgat gatggcaact cagatgttcc tcgacggcat
    11341 tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa aagagcccgg tagcgtcaca
    11401 actaccgggc gaacaaactt aactcaacag ctggaacgca atcatcccaa cgatggcacc
    11461 aacagtgccg aggatggttt ccatcatggt ccaggttttc agcgtttcgg cttcggtcgc
    11521 gccggtaaat ttaccgaaca accagaaacc agcgtcgtta acgtggctga caacaatcga
    11581 accaccagcg atacaaatcg acagtgccgc catttgcgca ccagagtagt tcagttgttc
    11641 aatgactggc atcaccagtc ccaccgccgt taaacaggct acggtggcag ccccctgaat
    11701 gatgcgcact gcagcagcca gcacgaagca ggtgatagca atcggcaggc ccatgccggt
    11761 taacgcttcg cccagtgccg gacctacgcc agagtcaacc agcacctgtt tgaacacgcc
    11821 gcctgcacca atcaccagca gaatgatccc cgccggttgc agcgcgtgac cgcaaatctc
    11881 catcactttg tcttttggca tgccctgacg cattgccagg ccataaatcg ctaccagaca
    11941 agcaaccaga atcgcggtaa acggatgacc aataaactcg aaccattcgt aagcggtaga
    12001 gccttctggc acaaaacgcg cggcaatggt tttcagcccc accagcacca gcggcaacag
    12061 gatcagcgac aggctgaatc cgaaagatgg cattttacct tcgccgagat gcggttcgct
    12121 gatgtcgtca ggaatatgca gctcaacgta acggctgatg aagttacccc acagcggccc
    12181 ggcaataatc attcccggaa ttgccgcaca caggccaatc aggatcatcc agccaaagtc
    12241 ggcattcatc tgcgatgcca gcagcattgg cgctggccca ggcaccagga acgctgccgc
    12301 ggccgccacg cctgcgaata atgggattac cagcttcacc aggttcgtac cggtgtggcg
    12361 cgccattgag aaagcaacgc taatcagcag aacaatcgcc acttcaaaga acagcggtag
    12421 cgcacagacc agccccgcaa ggccgatggc ataatgcgcg cggctgtgac cgaaggattt
    12481 gagcattttg acggcaatct gatcgactgc gccggtttca tataagatct tgccaaacat
    12541 agctcccagg gcgacaacca ccgccaggaa gccgagggtg cctcccatcc ctttttccat
    12601 cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg ccagccccca tggacaccac
    12661 catcaaagcc aggaaagcgt gcatacgcgc cttcatgact aaaaacagca gcagtaaaac
    12721 agaccctact gctgttaaaa caagcgttaa tgtagtcact acttatttgc cttttttaat
    12781 aacctcaatg gtgcttgcca caacaccttc cagcggttga tcgatatcca ccaccagtac
    12841 atcggtttcg tccgcacccg gctcctgcag cgtttcaaac tgcgtcacca acatttgggt
    12901 tttaaagaaa tggcctttgc gcgctttcag gcgactttca atcacatcaa aatcgccttt
    12961 caggtagatg aaagagagat tcggattacc ttcacgcagc aagtcgcgat agtgcttttt
    13021 cagtgcagaa caggcgatca gcgacacttt attggtgcgc tgcatagcaa acgcggagtc
    13081 gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc agcggttcgc cagacgccat
    13141 tttttcgata ttgcgccgtg gatggaggaa atcgccatca agaaacgcgg catgaagttg
    13201 atgcgccact tcactggcga ccgcagattt gccgctgccc gatacgccca tcaagacgta
    13261 aatgtggtga tcatggttag tcgtgctcaa agtggtgccc ccacaataca agaattaaaa
    13321 atgttacggg taactgttat cggcaacatt gtccagccgg acaatatcag aagcaatatc
    13381 catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat ttgtgggctt aaatagatcc
    13441 gcccggtgac aaggtgaaac ctaaatctaa cattttcggt gtcacagatt cgccacgaat
    13501 acgcgccagc aggcgttcag cgccaatact gcccatccgc tcacgcggcg tcagcacgct
    13561 cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg tggaaaccgg caatcgccat
    13621 atcgtcagga acttttaacc ccagacgctg acattcaaac gccgcgccga ccgccagatc
    13681 atcattagta cagaacacgc catccagctg cggatattcc cgccgcgcct ggcgaatcag
    13741 ttcaataccg gaagagtaag aagaagattg ctcaaccatc acgctatatg gcaccaggcc
    13801 tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg atagtacgtt cgtcgagacg
    13861 tgcgccgaga taggcaatgt ggcgatgccc acgagcaata atggcggtag tcatctggcg
    13921 tgccgcttca aagttatcaa aaccgacggc gatatcgagg cacggcgact ggctgtccat
    13981 caactccacc accgggatcc ccgccacttc aatcatcttt aaggtgcgcg gcgtgtgggt
    14041 acgttcggtg aggatcaggc cgtcgatatt ccaggagagc atggattcga gacgttcttg
    14101 ctccatttcc ggtttataac cgtagtgcgc cagcatggtc tgataaccgt gcgcgtcggt
    14161 gacgctttcg attccgcgta atacttccgc gaaaacctgg ttggtgagag aaggtaacag
    14221 gacgccaatc gcccggctgg tagcgttaga gaggatatcg ggcgcacgat tgggaatata
    14281 gcccagttca tcaagagcgg cggcaatctt gccgcgtaga gcgacggaaa cctgctccgg
    14341 gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct acacggtcag ccacatcctg
    14401 aagtacgggt cttttctttt tcatcgtcct gaaggtacaa aagagataga ttctcttagt
    14461 ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg cttgtttaca attattagat
    14521 atacactaaa tcaattgcat cgcattgtgc taacgacgcg gcaaaacccc ggaagcgtgc
    14581 atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa ggcaggcttc aaagatgccg
    14641 ttgagtttta aaccggcggc agatcgaaca gtaacacttc gctgtcgcta tccgcatgga
    14701 tggagattgc ctgctcatcc cagattgcca gaccatcgct ggtcgaggct ttcacgccgt
    14761 taatggtaac attgcctttt accacctgaa tccagacgcg gcgttcagcg gcaatctgat
    14821 gcaccgactg ctcatctttc agcaacgccc aacgatacag ttccatatcc tgatgcactt
    14881 tcagcgaacc atctcgcgca tccggcgaga gcaccagctg tttgccctgc acggcatcga
    14941 agcgacgctg ttcataacgc ggcgtaatgc cattttcttc aggcatgatc cagatctgat
    15001 acagatgcag acgctcggtg ctgcttgggt tgtactctga gtgacgaata cccgtacccg
    15061 cactcataat ctggaactca cccgccggaa cctgctcttt attgcccatg ctgtcctgat
    15121 gctcaacagt accttccagc acgtaggtca aaatttccat atctttatgc gggtgagtgc
    15181 cgaagccctg ccctacttca atcacgtcgt cgttaatcac gcgcagcgcg gagaagccca
    15241 taaagttcgg atcgtaatag ttggcacaag agaaagtatg ccaggagtcc agccagccat
    15301 gatttgcatg accacgttca tttgctttgc gtaagtagat catttgtatt cgccccctga
    15361 atgatttcga tgggcttagt gtggacccga tctgcctggg atgatagagg gtgaaaattg
    15421 ccccctctgt tcaaaaaaat tgaactattc gaggggccat caaacttaat tcgcgagagt
    15481 cactgtggag ggagaggctt gctcaaatcc gcgttcaagg atttccagat tggtaagaac
    15541 ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg gtttgataca acgcatcata
    15601 aacacgcccg taatcgccca tctccggctt catctcttct ctgaccgtca caccctcgtc
    15661 attgacatac tccagcacac cgaccgaatc atccgctgcg aatcccggtt cgcccggcat
    15721 aatattagcc ttcaggctgg tttcctgctg gtcgataccg tatttaataa acgaaccttt
    15781 cttaccgtga acgataaatt tcggataatc gattttcacc agatggctgg ttttgacgat
    15841 ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg tcgtcaggat tggctttatt
    15901 acgcaggctg cggatgtcat aagcgacgtg atccgggcga ccgaacagag aaataatctg
    15961 gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc tgcggcagtc caggtttggt
    16021 ttctgccacc gggcggtaat agtcaaaatg gctttccact tcgacaatct ctcccagctt
    16081 gccgctttca atcgcttttt tcgccgtcag gaagcaggaa tcaaagcgac gattctgata
    16141 tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac agctctttcg cctgcgcaag
    16201 tgtcggagtg aacgtttttt cgaccagcac atttttcccg gcttccagcg cgcgcttcgc
    16261 gtactcgaag tggctgtccg catgggtgca gacaacaacc aacttaacat cgggatcgtt
    16321 tagcacttcg tcgagatcgc tggtgaaatg gatatgagag taaatgggag cctgttcttc
    16381 cggcttcgca tggcgacgaa aaatatgcgc gacatgccag ctatccttgc ggttaagtac
    16441 atacggcaga tggtaacggg tggtgctttt gccgaagcca ataaaggcgc agttgatgac
    16501 catgatttcg tcctttttaa ggtggttatt tacaccttag cgcaaagcgg acgtggttcc
    16561 tactggagtg cgcataacga acacaagcac tcccgtggat aaattgagaa cgaaagatca
    16621 aaaaaaaagc cagcacccgg ctggctaagt aatactggaa gcaatgtgag caatgtcgtg
    16681 ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca ataataatca ttctcattcg
    16741 cacttgtcca acactttttg caaaaaaatg catttgactc gcatttgaaa gtcaatgatg
    16801 ttgaaaggga catttacccc aaagaggaca aaggaatgag tgagatagta atacgccacg
    16861 cagaaacacg ggattacgag gccatcaggc agattcacgc ccagccggag gtgtattgca
    16921 acacactaca ggtgcctcat ccttccgatc atatgtggca ggagcgactc gccgatcgtc
    16981 ccggcatcaa gcaactcgtc gcctgtattg atggagacgt cgtgggccat ctcaccattg
    17041 acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt tggtatctgt gtcgactctc
    17101 gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca
    17161 actggttacg ggtagatcgc attgaactaa ccgtgtttgt cgataacgcg ccagcaatta
    17221 aggtctataa aaaattcggc tttgagattg aagggaccgg taagaagtac gcattgcgta
    17281 atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa atagagaatt tgcaatctaa
    17341 gtttgtcatt taagccctca cctaccggga gggcttaaga tattaatacc ccgccgttaa
    17401 atcatccacc gagcgcgggt cggatgcgcc gtacaactca ccgtccggcc caaccataat
    17461 gctttgtgtg ctgcccatcg cctctttcag cgccactttc tgaccttttg cttccagcag
    17521 cttgagcgta tccgggctaa accctttttc gacacgcagc tcgtccggca accactgatg
    17581 gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg ccataatcga tgctattcac
    17641 caccatttgc agcaccgtgg tgataatccg gctaccgcct gggctaccgg taaccagcca
    17701 ggttttaccg tctttcacca caatggtcgg cgacatcgac gacagcgggc gtttgttcgg
    17761 cccgacggcg ttggcatcac cgcccaccag cccgtaaacg ttcggtacgc ccggtttggc
    17821 ggagaaatca tccatctggt tattaagcag aataccgctc tcacccgcga caatacccgt
    17881 accgaaggtg gtgttcagcg tataggtcac cgccaccgcg ttaccgtctt tatccactac
    17941 agagtaatgg gtagtttgat tactctcata aggcgcaagc ttgccagggc gaatctcact
    18001 ggacggcttc gcttttttga tatcaatttg atcggcaatg gatttggcat aggctttatt
    18061 ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg ccaagatatt ccgagcggtc
    18121 ggcgtaggcg tatttctccg cttctgccat gatttgcatc gcatcggcgc tgccaaagcc
    18181 gtatttcttc atatcgaagt tttccagaat attgaggatt tgtacgatat ggatcccgcc
    18241 ggaggatggc ggtggcatgg agtaaacctg atacccgcga taatcgccgc ttatcggagt
    18301 gcgttcgacc gctttatagg ccgctaaatc ttctttagtg atcaagccac cgtttttctg
    18361 catctcctgg gcgatctgtt ccgcaatcgt gcctttatag aattcgtccg ggccgttttc
    18421 agcaatcatc tccaggctct ttgccaggtt cgcctgcacc agcgtgtcgc cctttttcag
    18481 cggctcgccc tctttccaga agatagcttt actgttttcg tgattcggca acacttcgct
    18541 accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg ataaaaccat cgcgtgccag
    18601 tttaaacgcg ggctgcacga ctttgttcag cggcatggtg ccgtatttat ccagcgccag
    18661 cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga tgcgaagtga gtgatttttt
    18721 gctgtccggg ttgccctgat catcgaggaa catatcgcgg gtcgctttgg cgggtgccat
    18781 ttcgcggaaa tcgatagccg tggtattgcc atttttcgag cggattaaca taaaaccacc
    18841 accgcccaga ttccctgcct gcggatgcgt taccgccagc gcgtagccca ccgccacggc
    18901 ggcatcaacg gcattcccgc cctccttgag gatatccacc cccacctgag tggcagtggc
    18961 gtccacagac gctaccattc cctgtttcgc gcgtaccgga tggaagacat cttcctccac
    19021 accatacgag acgggcggcg caggaggcgc ggcggcggcg ctaaaacaac ttcctgagag
    19081 cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt atcatcgtta ttctccagag
    19141 attaaggggc aaccccagct aagcctggta tataactctg aattaatcat cgttttgtcg
    19201 ggaagcgagt aaacttaaag gatatcttca gaggaggata cgcgatgaaa cgacttctga
    19261 ttcttacggc actcctgccg tttgtcggct ttgcacagcc cattaatact ctgaacaacc
    19321 ctaaccagcc ggggtatcag atacccagcc agcagcggat gcaaacccag atgcagactc
    19381 agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa aacgcaaact cagttgcaac
    19441 agcagcattt agaaaaccag ataaacaata attctcagcg ggtgttgcag tcacagccgg
    19501 gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac caacggcggg atgttaaaca
    19561 gcaaccgtaa tccggatagt tcgttgaatc agcagcatat gctgccggag aggagaaaca
    19621 gcgacatgct gaatcagccc agcacgccgc agcctgacat tccgttgaaa actattgggc
    19681 cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag atgcaatcca cgccccagcg
    19741 cagcaactct gccgcgtgct ggggtttatt gacggtataa accagaatcc gtaatccggc
    19801 gtctttcaac tgcatcactc gcgctttatc gagtaactta tgattgagat gaatagagac
    19861 gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc cactcatcaa gcaataaacc
    19921 acgcggcagt tccggtgccg cctgttgtgc agcttctaaa gcatcaatct caaacgatga
    19981 cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc gccagcgcca ccattttgcc
    20041 cgttaatggt ccagtgccag tggtgggttt gatttcgata ttcgccatca tcccgtgttc
    20101 gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc tcacctttaa acgctttgct
    20161 gtaccaactg cccgcatcca cgcgcagtaa atcctgccag ttcagttcac ccgcgacgcc
    20221 ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc aggaagatct cgccatcttt
    20281 cgataacttc gcgtcaaatt cgatcatctt atgaccgtat tttgccccga cgtcgattgc
    20341 cgccagggtg ttttccgggg ccagcttacc gccgccacga tgagcgacga tgcggggata
    20401 aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa aaagatgcag ctgattttcc
    20461 gccagatgca gccacagcgt gctgcctgcc gtcgggcgct cctgatgcgc cagtcgcacc
    20521 accagcttct gcttgcgagc ttagcgcaat atgttccggg cgaatgccga gggtcatttt
    20581 acgcccggca tactgacggt agccaccgtt tagcggtagc acgataccgc cgtccagttc
    20641 gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg ttcatcgccg ggctgccgat
    20701 aaaactcgcc acaaacaggc tggcgggctt ttcgtagact tccaccggcg tgccaatctg
    20761 ttcggcaacg ccgccgttca tcaccattac tcgctgggcg agcgtcatcg cctcaacctg
    20821 atcgtgagta acgtagagtg aagtcgtttt caggcgacgg tgcagctgtt gcagttcaag
    20881 acgcatctgt acgcgcagct tggcatcgag gttagagagt ggctcatcaa acaagaacac
    20941 cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc tggcgctgac cgccggaaag
    21001 ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga atgcgcgccg cttctttaac
    21061 gcgctcggct atttgctgct tgcccatgcc gcgaattttc agcccccacg ccatgttttc
    21121 ttcgacgctc atatgcggat aaagcgcgta attctggaac accatcgcaa tcccgcgatc
    21181 tttcggctcc atttcggtca cgcgctggtc gttgatccag atatcgcctt cggtcaccct
    21241 ctccagcccg gcaaccatac gcagcagcgt cgatttcccg cagccagacg gcccgaccat
    21301 cacgataaat tcgccatccg ccacatcaag ggtcagcggt ttaatgacct aggttttgcc
    21361 atcccagctt ttggttactg cctgtaattt cagtcctgcc atcttatttc tcactatcga
    21421 ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat cactactggt gggatcaggg
    21481 ttaacaacat cgccgccatc actgagttcc attccgtggt gccttcgcca gtggcgatca
    21541 tccctttgat ccctgccacg gtggtgccga gatccacgtc ggtaataatc aacaacggcc
    21601 acaaatactg gttccagccg taaataaagg tgatcacaaa cagcgccgcc agattagttt
    21661 tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg tgacgcaccg tcgatccgcg
    21721 ccgcttccac cagttcatcc ggcagcgtca taaagaactg gcgaaacaga aacgtggcgg
    21781 tcgccgaggc catcagcggc agcgttaaac cggcgtagct gtcgagcatc ttcaggttgg
    21841 cgatgacttc caccgtcggg aagatacgta cttcaaccgg cagcatcagg gtgataaaaa
    21901 tcatccagaa gaagaggtta cgtagcggaa aacgaaacca gacaatggca aatgccgaga
    21961 gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc catcacaaag ctgttaagca
    22021 acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt cacccagatg ttgtggatgt
    22081 tttccagcag atgcgtgccg gggatgagcg tcatcggcgc ggcgtagacg gcctgtttat
    22141 ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag gatcaccgcg atcccgagga
    22201 tcagcatggt atggctgaat atcgtcagcc acggacggtt ctcaatcatt ggtaacgcac
    22261 cttgctttca acatagcgga actgcaccac cgtcagcacg atgacgagga acatcagcac
    22321 catcgactgt gcggcagacg aagccagatc cagtccggta aaaccttcgc ggtagatctt
    22381 ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac gtggcggcgt cgatcaccgg
    22441 gaaggtgtcg aagaaggcat acaccaggtt cactaccagc aggaagaaac tcaccggggc
    22501 gataagcggc agcgcaatct taaagaagcg gcgaatcggc cctgcaccgt cgatggctgc
    22561 ggcttcgatc aacgaacggg gaatggattg cagcgcggca tagaagaaca gaaagttgta
    22621 gctgatttgc ttccacaccg aggcaaacac caccagaaac atcgcctgac cgctattttg
    22681 cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg gtgatcagcc cgcgaccggg
    22741 gttaaacagg aagatccaca atacggcggc aacggcggga gccacggcat acggcagcaa
    22801 cattaaggtt tggtagaaac gactgccgcg cacgatgtac tccaccagcg ccgcaaagaa
    22861 cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg aattttatcg tcgtccagaa
    22921 ggagtcgaga taatagctgt catgaaacag cgtgacgaag ttatccagac cgacaaactg
    22981 gctggagaaa ccaaacggat cgacgctttg tagcgagtac cacaacgctt cgcccgcagg
    23041 ccagataaaa aagataacgg tgatgatgag ctgcggcgcg accagcagat aaggcagcca
    23101 gcgcgagcgg aacaccggac gggatgatga cattacggtt aattcctgaa ctgtgccgga
    23161 tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag ataagcgaaa cgcatccggc
    23221 attacactga ttaagacttc gtcgatttct caaagcggcg cagtaactgg ttcccacgct
    23281 caactgcggt atccagcgcc tgctgtggcg tcttcttacc ggtccacacg ctctcaagct
    23341 cttcatccac aatcacgcgg atctgcggca tgttgcccag acgaagccct ttggtgaacg
    23401 gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc cgcacctggg tttttctcgt
    23461 aaaagccctg ctcacgggtc aggtcatacg ccgctttggt aattggcaga taaccggttt
    23521 tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa gtcgagaaac ttcgccacgc
    23581 cggtatacgt ttctttatct ttgccctgca tcacccacag gctggctccg ccgataatgg
    23641 cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat catacctacg ccgtagttaa
    23701 atttggcgta ctcgcgaatg ttggcaagag aaccggaaga ggcggtggtc atcgcgcaat
    23761 caccgttata gaacttctcg gtggattcat ccttacgacc gacgtagctg aagtcgccct
    23821 tcttgttcat ctcctcgagc atggcgatgt gtttcacctg ctccggcttg ttgaactcca
    23881 gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc aaacggcaga ccgttccagg
    23941 cgctaaagtt ttccagttgg atccagccct gccagccgct ggcgtagccg cacttaatac
    24001 cggaggcttt cagttttgcg gaatagtccg ccagatcctg ccaggttttc ggcggctgtt
    24061 ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta atagagaacg gaagtcgagc
    24121 tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc ggagtagtaa ccggaaaccg
    24181 tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc tttaaacacg tcatacaccg
    24241 gtttaatggc tttcgacgcc atcatggtgg cggtgccaac ttcataaacc tgcaaaatag
    24301 ccggagcgtt gccggtacga aatacggcaa tccccgcgct taaattctgt tcgtagttgc
    24361 ctttataggt cggtacaatt ttgtaatccg gattttcggc gttaaaacgt tgggccagag
    24421 aatccacctc tttacccagt tccccttcca tagaatgcca gaacggaatg gtcgtcactg
    24481 cctgtgcatt ccccattaac gccagtccga gcgccagtgc tgaagctgta taatgtaacg
    24541 gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa ttcacgcgtt ttatgctcgc
    24601 ggggtaacat gacatgctcg aattacagaa aaataacttt tttgttacat ttgtaagata
    24661 gtaaggtgtc agaaagatga caaggcggtg acggcgtggg tgagggaaaa tgggagatgg
    24721 ggcacggata agcggaaaaa tagaaggtct gaatcaaact ctacagatta ctcatcgttt
    24781 catgccggat gcggcgtaaa cgccttatcc ggcctacaag atcgtgcaaa ttcaatatat
    24841 tgcaactcac ccagtaggcc tgataagcgc agcgcatcag gcaattttgc atttgtcacc
    24901 tgtctcaaag gagtcttttg actccttatc aatcaacgtg ttattacccg cctaaatacg
    24961 cacttctcac tgcttcgttc gccagcagcg cgtcgccggt atcggagagc accacatggc
    25021 cgttttccag cacgtagccg cgatccgcca gctttagcgc ctggttggcg ttctgctcga
    25081 cgaggaagat agtcatcccc tgctcgcgca gctgctcgat ggtgtcgaaa atttgctgga
    25141 tgataatcgg cgcaagaccg agcgatggct catcaagtaa caacaaacgc ggattgctca
    25201 tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc ggacatggtg cccgcccgct
    25261 gaacacggcg ctcatgcaaa cgtggaaaca gttcatacac ccactttatg cgctcctgaa
    25321 actggtcacg ttcggcaaag aagccgccca tcgccaggtt ctcttccacc gtcatccgcg
    25381 agaagacgcg acgcccttcc gggacaatcg ctaccgcttc gcgcatgatt ttcgccgtct
    25441 gccagtcggt aatgtcttta tcatcaaaca caattcgccc gctggtggcg cgcggatcgc
    25501 cgcataacgt gccgagcaag gtggttttcc ccgccccgtt cgcgccaatg agagtgacaa
    25561 tctcgccctg attgatatgc aggctcacct catgcagcgc ctggattttg ccgtagtggg
    25621 cgctgacttt gtcaaaggac aacatgactt tttccatctt atgcctcacc taaataggca
    25681 cggatcacgt ccgggttatt acggatttgc tccggcgtac cgtttgccag tggcgtcccc
    25741 tgattgacca cataaattcg gtctgaaatc cccatcacca gcttcatatc gtgttcaatc
    25801 aacaaaatag tggtgttgtg atgattgcgc agttcggcaa tcagctcatc cagctctttg
    25861 gtttcttttg ggttaagacc tgccgccggt tcgtcgagca ttaaaatctc cggctgcgtc
    25921 accatgcagc gggcaatctc cagacggcgt tggtcaccat aggccaggtt actcgcctga
    25981 cggttggcgt gttccagcaa accaatgcgc tcaagccagg tggcggcgcg gtcgagcgct
    26041 tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc cagagaacag cccggttttc
    26101 agttgctgat gctgcgccac cagcaggttt tcaattaccg tcatttcacg gaacagacgc
    26161 acatgctgga aggtgcgcac cacgcccatg cgagcaattt gctgccccgg taaaccttca
    26221 agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt tgtagaaacc ggtcaggcag
    26281 ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta acgagacgat ttcctgcggg
    26341 tacagttcaa gattgacgtt gttcaccgcc agcaggccgc cgaagcgcat catcagaccg
    26401 ttaacagata ataatggctg actcatgcct gctctccttt cgctgcgccg tttttcagct
    26461 tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg ccagatcatc atcagcacca
    26521 tcaaaccacc gagcattaac atgctgtatt cgttgaaatc acgcatcaat tcgcgcgaca
    26581 ccaccagcag aacagccgcc agaatcactg caaattgcga gcccataccg ccgagcacca
    26641 ctatcgccag cacaaaggcc gattcagcaa aggtgaagga ttccgggctg acaaagccct
    26701 gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc ggcgctgatg gtaaaagcag
    26761 tcagcttgat acggcgcggg cttaagccca gcgaacgaca ggcgatctcg tcttcacgca
    26821 acgcttccca cgcgcggccc agcggcatcc gcagcaggcg gttaatgaca aacaggctta
    26881 acaccaccag cagcaacgcc accaggtaga ggaagataac gcgatcggac ggatcgtatt
    26941 tcaggccaaa gaaattactg aacgtgtccc agccgccttc acgagcggta cggctgaact
    27001 cgagtccgaa gagtgtcggt ttcgggatct gactgattcc gttcgggccg ccggtgattt
    27061 cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa accaagggta acgatcgcca
    27121 gatagtcacc gcgcagacgc agcaccggaa aaccgagcag gaaacccgcc gccgctgcca
    27181 tcagtccggc aatcggcaaa caggtccaga accccagacc gtaatagtga ttgagcagcg
    27241 caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc cagcaccagc agaccagaaa
    27301 gaccaacaac cacattcagc ccgagaccga ggataatgta gatcatggtc agagtggcga
    27361 tatccaccgt cccacgtgac accataaacg gccacgccac cacaagcacc agcagcgcca
    27421 cgaggaacag cttttgcttc accgtggacc catcaatcgc gggcagaata aacttcggcc
    27481 cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa aagctggaag aaaaagacca
    27541 ccgccgtgcc gataaacatc cactgccaac ggatgtctga agccgtgtcg accaccagtt
    27601 tggtgccatc cagctccagt tgtacgccca taaagacgcc cgccaggaca aagaacatcg
    27661 cggcagagag cagtgccatt gcaatatgca tcggtttcat actttctcta cctccgggcg
    27721 acccagaata ccggtcggca tcaccagcag caccagaatc agcagggcga atgagaccac
    27781 atctttatat tccgtactca gataggcaga agagagcgcc tccgcaatcc ccaggatcag
    27841 cccgccaatc atcgcccctg gaatgctgcc aatcccaccg agcaccgcag cggtaaaggc
    27901 tttcatcccg gccataaagc cgatataggg gttaatgacg cagtagaact gaccgagcag
    27961 tacgccagcc actgccgcca tcgccgcgcc aataacaaag gtcaaagcaa tcacccggtc
    28021 ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc gcgcaggcac gacaggcgcg
    28081 ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc atggcgagga aggtgacaat
    28141 ccagatcacc gcctgcatgg tggtaataga ggcagagaag ttttcgctat ggcccaccac
    28201 ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc gaaccttcgg tcaggctgac
    28261 gtagttttgc aggaagatgg acataccgat tgcggaaata agcgcaatca ggcgcttaga
    28321 gttacgcacc gggcggtaag ctacccgttc gatactccag ccgtaggcgc tggcgatgac
    28381 gattgcgccg acgaatcccg ccgccaccag cagccagccg gtatcaatgc ccatcatcat
    28441 cagcgcggcg atgatcataa atgagacgta gctgccaatc atataaacct cgccgtgggc
    28501 gaagttgatc atgccgataa tgccgtaaac catggtgtag ccgatggcta tcagcgcgta
    28561 ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag aaatacaaaa actgctcaga
    28621 cataaggtaa cctttctaaa cccgcccgca ttttacgggc ggtgggatga tcacttggct
    28681 gccgtggatg aaccgtcggc gtgccactgg aagacaccaa aatcaaatcc cttaagatcg
    28741 cctttttcat cccagttcag cggcccaatc acggtgtttg caccgttagc ttttaaatct
    28801 ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa gggcagttgc cagagattgc
    28861 actgccgcgt aagtgatcca gacataaggc ccggacggat ctttcttgtc tgctttcagc
    28921 gcatcaacga tgccctggtt tgccggatcc tggtcatagc gttttggcat agtgaccaac
    28981 atgccttcgg cggcatcacc ggcaatgttc gacagcgatg cgttaccgac accttccggc
    29041 cccataaact gggttttcag gccaacggaa cgggcttggc gcagcatctg ccccatttcc
    29101 gggtagtaac cgccgtagta aacgaagtcg atattttctt ttttcaggcg ggcgatcagc
    29161 gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga agacgacgtt ggcgttagcc
    29221 gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc catactgttg tttgtcgtga
    29281 atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt attttgccgc cgtaggcccc
    29341 tgggaagagt ccagcccggc agtacgcata atgtgttgat aaccgcgttg ggtcagctcc
    29401 gggttggtcg ctcccggcga gatcatcaga ataccttcgt cttcgtagat atccgacgct
    29461 ggctgggtag aggaagaaca cagatgacca ataacgtatt taatgccgtc gttaacgatt
    29521 ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt cgtcatattc cacgccaacc
    29581 agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt taatcgcctg acgcgcgccg
    29641 ttaaattcca tatcgcccca ctgggcaatc gggccggaca tcgcgccgac aacggcgact
    29701 ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg caatcatccc tgcgatgata
    29761 gttttcgcat tccgtttcat agtcaaaaat ccccattcgt gatgttgtgt tgctttgttt
    29821 ttatgtgtta acaaatcaga ctgttctttt tttatactgc actgtttttg cctgtctgat
    29881 taaggggtta gcgcagtatt ttgtgatgat agcgattaaa atccctattt ttcagtcgat
    29941 taagaacaga taatattctg aatttattga tagataaaca gaaaaaagtg cctttgtcag
    30001 cataaaataa cggcacaaag ggcggaataa ttcactatca ttcaggggat tatgctggac
    30061 atttttcatt ctctaatgtt ttaactttgt aattattgct gttaaaaaat taatcacctg
    30121 ccaaaagaaa taaaaaagag aaagcctccg attaaattat ttcgctacac tggttccact
    30181 tttgtgattt acacgggtta cccatgaagc tgaccatcat tcgattagaa aactttagcg
    30241 accaggaccg gattgacctg caaaagatct ggccggagta ttccccttcc tctttacagg
    30301 ttgacgataa ccaccgtatc tacgccgcgc gttttaacga gcgtctgctt gcggccgtgc
    30361 gggtaacctt aagcggcacc gagggagcac tggattctct acgcgtgcgg gaagtcaccc
    30421 gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt gcgtaacaat cctggcgttt
    30481 catgctggtg gatggcggat gcaggcgtgg aagatcgcgg tgtgatgacg gcgtttatgc
    30541 aggcactggg atttacggca cagcagggcg gctgggagaa gcgttaatcg tcaagttgat
    30601 ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta cgcttatcag gcctataatg
    30661 tgtgctgcaa tttactgatt tctttggttc ttgtaggccg gataaggcgt ttacgccgca
    30721 tccggcatga agcaacgtat tcgatattag caatttggcg gcaacccaaa gttgccgatt
    30781 aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc agtcaaatac gccgaactca
    30841 aagcccttta gatcgccttt ctcatcccag gtcagtggcc ccatcacggt atccacggag
    30901 ttcgctttta ggtatttggc gatttcagcc ggatcgtcag actgattcag gcccgcctgc
    30961 aaagattgca gcgcggcgta ggtggtccag acgaatgcgc cgcttgggtc ctgttttttc
    31021 gctttgatcg catcaacaat gggtttgttc gccggaacct gatcgtagtt cttcggcttg
    31081 gtcaccagca gcccttccgc tgattcgccc gcaatgttag acagcgaaac gttagccaca
    31141 ccttccggcc ccataaactg agttttcagc cctgccgcgc gtgcctgacg caggatttgc
    31201 cccatttccg ggtgataacc gccgtagtaa acgaagtcga tattctcttt tttcagacgc
    31261 gccaccagcg ttgagaaatc tttttccccg gcggtgatac catcaaagaa caccacgtta
    31321 gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca gaccttcgcc gtattgctgt
    31381 ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct caagaatata tttcgccgcc
    31441 gtcggcccct ggtcggagtc caggccggtg gtgcgcagga tcagctgata gccacgggcg
    31501 gtcagctccg gcgcggttgc cgctggggtg atcattaaaa tgccttcgtc ttcgtagatg
    31561 tcagacgcag gctgcgttga tgaagaacag aggtgaccaa tcacatattt aatgccgtcg
    31621 ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt cacaggcatc gtcatatttt
    31681 acgatttgca gtttgttgcc tttaatgccg cctttagcgt tgatatccgc aaccgcctgc
    31741 tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg gaccggacat tgcacccacg
    31801 accgcgactt taatatcttc tgccagagcc atattgctga atgccagcgc gatacatcct
    31861 gccagtaacg ctttaccctt tatgttcatc ctgagaatcc ccattcttct ggttattacg
    31921 tgtgttgtga tgttgttgtt cagcacttta tttcgtttta tgcatgacta cccgtgcttt
    31981 agcagcatac tctgctaaaa catacccgat ttttatgata ttggaatagc tattttgaca
    32041 gtttattaac aatctgcgtg gggattggcg ttttgccgga gggggaattg attattgtag
    32101 aggcccaaaa aacaaaaacc ccggactctc atccagggtt ctctgcttaa tagcggaaat
    32161 tacgcttcaa tagcggcgcg aagttttttc atcgcgttct tttccagttg gcgcacacgt
    32221 tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg tggacttgtt gtcttcgtcc
    32281 agccagcgcg cacggatgat gtcctggctg cgttcgtcca gaccatgcat cgcgtcggtc
    32341 agacggtttg ccgcctgctc ttcccagtta tcatcttcga tgccgtcggc aaagttagat
    32401 gatttatcct gcagatagag caccggtgcc atcggctggc tgtcggaatc gtcgtcggaa
    32461 gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt ccatctcacg tacgtctttg
    32521 ctggttacgc ccagttcacg ggccaccatt tcgacttcat cctggttaaa ccagcccaga
    32581 cgctgcttgg ttttacgcag gttgaagaac agtttgcgct gcgctttggt ggtcgcaact
    32641 ttgacgatac gccagttacg cagaacgtat tcgtggatct ctgctttgat ccagtggacg
    32701 gcgaaggaga ccaggcgcac acccacttcc gggttaaaac ggcgcactgc tttcatcagg
    32761 ccgatgttac cttcctgaat caaatccgcc tgtggcaggc catagcccgc ataattacga
    32821 gcaatatgaa caacaaaccg caggtgagac aggatcagcg ttttagctgc ttccagatcg
    32881 ccatggtaat gcagcttttc agccagcgcc cgctcctcgt cagccgacaa catcggccac
    32941 gcgttagctg cccggatgta ggaatccagg ttgccaactg gggctaaagc taaactttgc
    33001 attttgtcag tcattcaaat cctctcaatc gatatcttct ggcgcttcag tggtagcaac
    33061 aactgtgcca aagcttaaga gcaacgaggt tatcattcac tgttttatca gaccgtgatt
    33121 ttatccacaa gttcaatgca agcttgtgaa taaattacgc acaaaatgtg acatagagat
    33181 gaaataccgg gaagagacaa cggggtctct ttccctgcta cggaacccat tgcagggaaa
    33241 gagtataaca cgcttttatt attcaggcgt aaagtggcgt aaatgttgta ccgtggcaag
    33301 ccacgctgcc acccagccaa tcatcgagca taccagcagc aatagcaggc attcatcgaa
    33361 cgataaacca ttgatatcaa acttcgttcc gaaaacctgt gccacttccg ccaccgctga
    33421 cgacaaacgc agtaccagaa tttctgacaa aattaatgac aacaatgcgc cagaaaatcc
    33481 cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa ccatccgtcg caccaatcag
    33541 tttctgcacg ttgatggagt cacggcgagc aaagatgctc agacgtacgc tgttgccgat
    33601 gacgaggaac actgccgcca ccatcaacac gccgatcatc gccgaaacgc gcccaaccag
    33661 cccggtcaac gccgccagac gggcaaacca gctgtcatcc atccgcactt cgtcaatacc
    33721 gttaatctgc gtgatacgat cacgcagcgt attgagtgat tccgtcccct gaaaatcgag
    33781 tttcgggatc accaccgcca ctgccggaag cgggttttct tccagcatat ccagcgcacc
    33841 accaaaacca gaccagttac ggaactcgcc cagtgcgtct tcacgagaaa gatagttcac
    33901 tttctccacg ccttgctcgg cctgcaactg tgccaccacg cccgcagcgg cgtcatcgtc
    33961 cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga taatactgcg tcgccgcctg
    34021 attaacgttt ttgtacacca tataacagac gctgggcagc gtcagggaga tggcgataac
    34081 catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga tcctgcaatg cgccgtggaa
    34141 ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga tttaccggtt ttggcgagga
    34201 tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag ccgccgaccg atttacggaa
    34261 gcgatccaga cgcccaccaa actgccgaat atgattgatt gcatcgcgct tattcatggc
    34321 ccacgcctcc atgcaagtga ccatcgctca gggtgagcat gcgataggaa cgccgtgaaa
    34381 tcagattaat gtcgtgcgtt gccatcaata cggttacccc aacgcggtta aactcttcaa
    34441 acagacgtag aatcccttcc gacaacgcgt cgtccaggtt accggtcggt tcgtccgcca
    34501 gcagtaccgc gggcttattc accaccgcgc gggcaatgcc aacacgctgt tgttcaccgc
    34561 cggaaagctg aatagggaag ttcttcgctt tgtccagtag cccgacttta tccagtgccg
    34621 ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc gataatcagc gggatcgcca
    34681 cgttatcgta gacagtacgg tccatcagta gatggtgatc ctggaaaatc atgccaatct
    34741 ggcggcgcag aaacggaact tcacggtttt tcagacgcgt gatgtcatgg ccgctaaacc
    34801 agattttccc ggcgctgggc cgctcaatcc cacagatcag cttcaggagg gtacttttcc
    34861 ctgcgccgga atgaccggtc agaaacgcca tctcacccgg ctgcatatgg aacgtaacgc
    34921 cctgcagcgc ctgtctccca ccgagataag ccttgctgac atgttcaaag cgaatcattg
    34981 ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc gccttaaacg gacgcaaatc
    35041 ctcaatacgt tcgccgacac caatgtagcg gatagggata ccaaactggt cagccaccga
    35101 gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt agcgtgatgc cggttaagcc
    35161 aacagcttcg tggaacagtt tggcctggct taccgcgttc tgcccggtgc tggcatcaat
    35221 agtcagcata acttcatgcg gcgcttcaac gtcgagtttc ttcatcacgc ggacgatttt
    35281 cttcaactct tccatcagat gcgatttgtt ctgcagacgt ccggcggtat cggcaatcag
    35341 gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg aagataacag aggcggaatc
    35401 cgcaccggta tgctgggcaa tcaccggaat attgttgcgc tgaccccaga cctgaagctg
    35461 ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc atcaccgatt taccctgctg
    35521 ctcaaactga cgcgccagct taccaatcgt cgtggtttta cccacaccgt tgacgcccac
    35581 catcaggatc acgaacggcg ttttatcttc aacattcagc ggctcatcga ctttcgccag
    35641 aatctcgccc atctcttctt tcagcaggcc atagagcgcc tcggcatcac gaagctgctt
    35701 gcgggatgcg ccttccgtca gattggtgat aattttacgt gtggtttcca cgcccacatc
    35761 ggcgatcaac agctgttctt ccagctcttc aaacagatca tcgtcgattt ttttaccgcg
    35821 gaacaggctg ataaatccgg aaccgagatt ttctttggtt tttaacaggc tgcgtttcag
    35881 gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc tgggcaattt ctgcaaccgg
    35941 ctgctcttct tctgccggag gaactaccat caccgcctct tctgccgctt cggcagccag
    36001 cgcctgtgct tccagctctt cgtcggtgat ttcttcttta gccgcttctt cttctgccgc
    36061 ttcgacaatc tctacggttt ccgcttcagc ctgccactct tctgacgaaa cctcttcggc
    36121 gttgacgtct tccggcaacg gcagctcttc acgttcgata gccactggct ccggcgtttc
    36181 ttctacgacc ggttccggct gtgcaacaac ttccgcttca ggctgcgctt tttcactttc
    36241 aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa gtttccgcct cggcttcagt
    36301 atgcgcctgc ggctgctctt caacggcttg ttcagaagcc ttcgcaggct cttgcgcctg
    36361 aacgatttct tctacaaccg gttgttcatt ctgaacttct gtctcttttt ccggggtctg
    36421 ctctttttga ccaaagccca gccaggaaaa aaagccacgt cttttttctt tcgccatttg
    36481 cgactacact cctcgctgtt gcttcatggc acagcgttaa cgctatgtac atagcagcta
    36541 aaaaaatgat gaaatagtct atcacttaac ttaattcaca tcaccgcctg caagtatgtg
    36601 ttatctggcg gattgagcaa tttatcatga aaaaaccgaa tcattccggc agcggccaaa
    36661 tccgcattat tggcgggcag tggcgaggcc gtaaactccc ggttcctgat agcccaggtc
    36721 tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa ctggctggct ccggtcattg
    36781 ttgacgccca atgtctggat tgcttcgccg ggagcggcgc gctggggctg gaagcgttat
    36841 cgcgctacgc tgcgggggca acgttgattg agatggatcg cgtggtttct cagcagttaa
    36901 ttaagaatct ggcgacacta aaagcaggca atgcacgcgt ggtgaacagc aacgcaatgt
    36961 cattcctggc gcaaaaaggc acaccgcata atatcgtgtt tgtcgatcca ccgttccgcc
    37021 gtggcttgtt agaagagacg ataaatttac tggaagataa cggctggctg gctgacgaag
    37081 ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc cactgttccg gcaaactggt
    37141 cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg gctgtatcaa cacgaagcac
    37201 aaggagaaag tgatgctgat taatattggt cgtttgttaa tgctctgcgt ttggggattt
    37261 ttaatcctca atctggtgca tcccttcccg cgcccgctaa atattttcgt taacgtggcg
    37321 ctgattttta ccgtgctgat gcatggtatg cagctggcgc tattgaaatc cactttaccg
    37381 aaagatggcc cgcagatgac caccgccgaa aaggtgcgga ttttcctttt cggcgtgttt
    37441 gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat aaccttattg ttcgccaaca
    37501 aagctaacaa agcgcgttcc tttatagctc agcgtacctt tatcgcccac tgtcagggcg
    37561 tggtactgct gggcgtcgag gcgaaacgtc tgctccattc ctccgctttg cggtttgaag
    37621 cttgcctcat agcgcatact ggtgcctgcc ggagtcactt cctgctggcg cgaacggcga
    37681 tcgttaatcg gtttttcccg cttgttgctc accaccacca gcttttgctg gagcggagcc
    37741 atatcattat cagctttttc ccgtcgctgt tgcataaaac gaaacgatgc ggcgacgaca
    37801 attaagccaa tgataacaat aaagaaaaga ggtggtttac tcatctttat ccctcatcgg
    37861 aaaatgcgga attaagcata ccctgccagt tatggtgttg tcatccgtcc accctcgcca
    37921 ctaaactgga agcaagaccg taggcattct gcttacgaaa aaataacgaa ttcaaggaac
    37981 taagatgctt tggtcgttta tcgctgtctg tctttccgca tggctatctg tggatgcatc
    38041 gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg ttaacccttc ttctcctgct
    38101 gttactggcc tggcaagcac cgatgttcga cgccattagc tatctggtgc tggcgggact
    38161 gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca cgtcaacgtc tgatgtacgc
    38221 catcggcgcg tttttcctct cgcacctgct gtacaccatc tatttcgcca gtcagatgac
    38281 gctctctttc ttctggcctc tgccactggt gttgctggtt ctgggtgcgc tgctactggc
    38341 gattatctgg acgcggctgg aagagtaccg ttggcctatc tgcacgttta tcggcatgac
    38401 gctggtgatg gtgtggctgg caggtgaact gtggttcttc cgtccgaccg ctccggcgct
    38461 ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac tttgtctggc tggggagcca
    38521 ctatcgccga cgcttccgtg cggataacgc gattgctgcg gcctgctact ttgccggtca
    38581 cttcctcatc gtccgctcac tgtatctctg ataaaacttg actctggagt cgactccaga
    38641 gtgtatcctt cggttaatga gaaaaaactt aaccggagga tgccatgtcg acacctgaca
    38701 atcacggcaa gaaagcccct caatttgctg cgttcaaacc gctaaccacg gtacagaacg
    38761 ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc aacgctctct gaaaacgtct
    38821 ccggcacccg ctatagctgg aaagtcagcg gcatggactg cgccgcctgt gcgcgcaagg
    38881 tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt gcaggtgttg ttcgccaccg
    38941 aaaaactggt ggttgatgcc gacaatgaca tccgtgccca ggttgaatct gcggtgcaaa
    39001 aagcgggcta ttccctgcgc gatgagcagg ccgccgacga gccacaagca tcgcgcctga
    39061 aagagaatct gccgctgatt acgcttatcg tgatgatggc aatcagctgg ggtctggagc
    39121 agttcaatca tccgttcggg caactggcgt ttatcgcgac cacgctagtt gggctgtacc
    39181 cgattgctcg tcaggcatta cggctgatca aatccggcag ctacttcgcc attgaaacct
    39241 taatgagcgt agccgctatt ggtgcactgt ttattggcgc aacggctgaa gctgcgatgg
    39301 tgttgctgct gtttttgatt ggtgaacgac tggaaggctg ggccgccagc cgcgcgcgtc
    39361 agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc cacgcgcctg cgtaacggtg
    39421 agcgggaaga ggtggcgatt aacagcctgc ggcctggcga tgtgattgaa gtcgccgcag
    39481 gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt tgccagtttt gatgaaagcg
    39541 ccctaaccgg agaatccatt ccggtggagc gcgcgacggg cgataaagtt cctgcaggag
    39601 ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc agaaccgggt gccagcgcca
    39661 ttgaccggat tctgaaactg atcgaagaag ccgaagagcg ccgcgccccc attgagcggt
    39721 ttatcgaccg tttcagccgt atttatacgc cagcgattat ggccgtcgcc ctgctggtaa
    39781 cgctagtgcc gccgctgctg tttgccgcca gctggcagga gtggatttat aaaggtctga
    39841 cgctgctgct gattggttgc ccgtgtgcgt tagttatctc aacgcctgcg gcgataacct
    39901 ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa aggcggtgcg gcgctggaac
    39961 aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg tacgctgacc gtcggtaaac
    40021 cgcgcgttac cgcgattcat ccggcaacgg gtattagtga atctgaactg ctgacactgg
    40081 cggcggcggt cgagcaaggc gcgacgcatc cactggcgca agccatcgta cgcgaagcac
    40141 aggttgctga actcgccatt cccaccgccg aatcacagcg ggcactggtc gggtctggca
    40201 ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc tgccgggaaa catcccgctg
    40261 atgcatttgc tggtttgatt aatgaactgg aaagcgccgg gcaaacggtt gtgctggtag
    40321 tacgtaatga tgacgtgctg ggtatcattg cattgcagga taccctgcgc gccgacgcta
    40381 caactgccat cagtgaactg aacgcgctgg gggttaaagg agtgatcctc accggcgata
    40441 atccacgcgc agcggcggca attgccgggg agctggggct ggagtttaaa gcgggcctat
    40501 tgccggaaga taaagtcaaa gcggtgacca agctgaatca acatgcgccc ctggcgatgg
    40561 tcggtgatgg tattaacgac gcgccagcga tgaaagctgc cgccatcggg attgctatgg
    40621 gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc attaacccat aaccacctgc
    40681 gcggcctggt gcaaatgatt gaactggcac gcgccactca cgccaatatc cgccagaaca
    40741 tcaccattgc gctgggactg aaagggatct tcctcgtcac cacgctttta gggatgaccg
    40801 ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt gctggtgacg gcgaatgcgt
    40861 taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac attgagcgcg atcggtcccc
    40921 tcgcccctct ggggagaggg ttagggggtg aggaaaaggc ggcatcgaag ccaatcagcc
    40981 cctatcaacc gcctttacga atcaaataac gataaggcag tccatccgtc tctttcgcaa
    41041 ccagttcgtg ttccataaag gtacaaaacc caggaatatc gcgggtagtg gccggatcgt
    41101 cggcgataat cagcaacgtt tcgccaggct gcatattgcg cacggttttg cgcaccatca
    41161 tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt gtggtcaggg ctggaaaaga
    41221 gatcggtcat tttcttctca tcacttaaaa aaacggcgct agtttacgcc ctgtgagtcc
    41281 gtaagcaaac caggttaacg attgcgtgaa aattaaccat tgcattgtca acgtaaagca
    41341 gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt atgttaaggt aacagacgtg
    41401 tcgtacgtat tgggttccct caccccaatg gttaatcaaa aaggtacaat atgaacgttt
    41461 tctcgcaaac tcaacgctat aaggcgttgt tctggttatc gttatttcat cttctggtga
    41521 tcacctccag taactatctg gttcagcttc ccgtctccat ttttggtttc cataccacct
    41581 ggggcgcgtt tagctttccg tttatttttc ttgctaccga cctgaccgtg cgtatttttg
    41641 gcgcaccgct ggcccgacgc attatcttcg cggtaatgat ccctgcgtta ttaatctcct
    41701 acgtcatctc gtcgctattc tatatgggtt cctggcaggg attcggcgca ctcgcccact
    41761 tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat ggcctacgcg ctggggcaaa
    41821 tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg ccgctggtgg ctggcaccga
    41881 cagcgtccac actgttcggt aacgtcagcg acacgctggc ctttttcttc attgccttct
    41941 ggcgtagccc ggatgccttt atggctgaac actggatgga aatcgcgctg gtcgattact
    42001 gtttcaaagt gttaatcagt atcgttttct tcctgccaat gtatggcgta ttactcaata
    42061 tgctgttgaa aagactggca gataaatccg aaatcaacgc tttgcaggcg agttaaaggt
    42121 tcgttatcag agttgtgata agatggatga atgagccgtt atggccgttt atcgaaagga
    42181 agaagtcaat gcgcaatctg gttaaatatg tcggaattgg cctgctggtt atggggcttg
    42241 cggcctgtga tgataaagac actaacgcta cggcgcaggg ttcggttgcg gaaagtaatg
    42301 ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag tttctcgctg ccagcggata
    42361 tgaccgacca gagcggtaag ctgggaacgc aggccaataa catgcatgtc tggtccgacg
    42421 ccaccgggca gaaagcagtc atcgtcatca tgggcgatga tccgaaagaa gatctggcgg
    42481 tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga tccgcagctg caagtggtaa
    42541 ccaataaagc cattgagctg aaaggtcaca aaatgcagca gttagacagt attatctccg
    42601 cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa cgtgggtaat caactgctga
    42661 ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc gcaaaccacc gcagaaaaca
    42721 tcattaatac gctggttatt cagtaagttt taagatgatg aggcggcctc agggacgtgt
    42781 tccggaggcc gtttttttaa tcgccacgtc agtaataacg cgattgcgac cagtcccgcc
    42841 gccgccagat aaatcaccgg tacgcccgcc cagctcatca ccagcccagc cagtggtcca
    42901 gtcacgccaa gcgataaatc cataaatacg gtgtaagttg ccagcgccgc cccctgattt
    42961 tgctgcggaa ccgcttttac cgccactaca cccaatgccg ggaacaccag cgaaaacccg
    43021 gcccccgcca gtaagacgcc gattttcgcc atccacggca tagtcgccac gccaaccagt
    43081 agcaggccga ttatctcaac gctaaagcaa atcatcgcta cgtttaagcc accgatacgg
    43141 ttaatgccgt tagggaataa caaacgcgta ccgacaaacg cacagctaaa cagcgtcagc
    43201 gcgaaagccg caccgtccca acctttagcg tcataaaaca gcgtgataaa ggtggcgatg
    43261 acgccaaatc cggcggaagc cagtgccagc gccataccgt acagccagac gcgcccaagc
    43321 accgcgcgaa acggcagcgg tttgccttta ctggctttta ccgtcggacg cgggatcgcc
    43381 aacaaaatgg ccaccagcgc cacgcccata atgattaacg ctaacgcctg caagccgccc
    43441 cagtgataaa acacgacgcc taacggcgca cccatcgcca tcgccccgta agtgacaatg
    43501 ccgttccacg aaatcacccg cccgatatgc agcgagccaa ccactccaac gccccatagg
    43561 gtcgatcccg ttccggcaaa actttgccca atcccaagga tgacgcgccc caggcaaagt
    43621 aataacaggc tgatgacagg cagactggcg gttaatcctg ccgtcagata ccccagaccg
    43681 ctcaaaaagc agccgcataa accgaagacg acaatctttt tgggtcccag caaatcggcg
    43741 taacgtccgg catgagggcg gctcagcaag gtggcgaaat attgcaggct gataaccaat
    43801 cctgcccaga aggcgctaaa gcccatcaca tcatggacat agcccggtaa tacagcgagc
    43861 ggcaacccga tggtgaggta gctggcgaag ttaaacatga caatggagac aatgcgcaaa
    43921 ttcaggcgca atccgtttag cgcgggttcg gctacgggtt cgggcatgag gatcaccaca
    43981 tttttacaac agtgtttcat ttttaccatg tgctgacgtg aaaatcagca gtaagaatca
    44041 gaatattgct ggcgtgactc ccgcgctaca cttaacacaa aaagtcacaa ggaagcccca
    44101 atggaaaccc ctcaacccga taaaacgggc atgcacattc tgctcaagtt ggcctcgctg
    44161 gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca ttgtgcagct gttactggcg
    44221 ctgttttttg ccatcgtcct caacccgctc gttacctggt ttattcgtcg gggagtacaa
    44281 cgccccgttg ccattacgat tgtagtggtg gtgatgctga tcgcactaac cgcgctggtc
    44341 ggcgtactgg cggcatcgtt taacgaattt atctctatgc tgccgaagtt taataaggag
    44401 ctgacgcgca aactttttaa attgcaggag atgttgcctt ttcttaattt gcatatgtcg
    44461 ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg ttaccttcac cacagcgcta
    44521 atgaccgggc tttccggggc aatggcgagc gtgcttttgc tggtgatgac cgtagttttt
    44581 atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt ttgcgctgaa taacccacag
    44641 attcacatcg caggattaca ccgcgcactt aaaggcgttt cgcattatct ggcgttaaag
    44701 acgctactca gtttatggac aggtgtcatc gtctggctgg ggctggcgct aatgggcgta
    44761 cagtttgcgc tgatgtgggc agtactggcg tttttgctca actacgtgcc caatatcggc
    44821 gcggtaattt ccgccgtacc gccaatgatt caggtgctgc tgtttaatgg tgtttacgaa
    44881 tgtattctgg tcggcgcatt gtttttagtg gtccatatgg tcatcggcaa tattttagaa
    44941 ccacggatga tgggccatcg cctggggatg tccaccatgg tggtatttct ttcattgtta
    45001 atctggggat ggctgctcgg cccggtaggg atgctacttt cggtgccatt aaccagcgtg
    45061 tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac tggcgatttt actggggccg
    45121 ggcagaccga aaagtcggtt accgggatga ggcgacaagt gatacgatac gcactttcat
    45181 tttccattaa acgttggccc tgatatgtat cggatagttc tggggaaagt ttcgacctta
    45241 agcgcagctc cactgccacc gggtttacgc gagcaagcac cgcaaggtcc acgacgcgaa
    45301 cgctggctgg cggggcgtac attgctttcg cacacgcttt ccccgctacc ggagatcatc
    45361 tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc cgctatggtt caacttaagc
    45421 catagcggtg acgatatcgc tctgctgttg agcgatgaag gcgaagtcgg ctgcgatatc
    45481 gaagtgattc gcccgcgcgt caactggcgc tggctggcga acgccgtgtt cagcctcggg
    45541 gaacacgctg aaatggacgc cgtgcatcct gatcagcaac tggaaatgtt ctggcgcatc
    45601 tggacgcgca aagaagccat cgttaaacag cgtggcggca gcgcctggca aatcgtcagc
    45661 gtagatagca cctatcactc ctcgctgtca gtcagccatt gtcagctaga aaatttaagc
    45721 cttgcgatct gcacccccac cccctttacg ctcaccgccg acagtgtgca atggatcgat
    45781 tcagttaact gatccgccca cccgactgcc catctattga tccagaacag gtaatcagta
    45841 tgacgaatac ttaaaatcgt catacttatt tccgccatct attttaatcc attggggtta
    45901 ccatgctctc cacactccgc cgcactctat ttgcgctgct ggcttgtgcg tcttttatcg
    45961 tccatgccgc tgcaccagat gaaatcacca ccgcctggcc ggtgaatgtc gggccactaa
    46021 acccgcacct ttacacgcct aaccagatgt tcgcccagag catggtttat gaaccattgg
    46081 tgaaatatca ggcagacggt tcggtgatcc cgtggctggc aaaaagctgg actcattcag
    46141 aagatggtaa aacctggacc ttcaccctgc gtgatgacgt gaaattctcc aacggtgaac
    46201 cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt gctcgataac cgtcaacgtc
    46261 acgcctggct ggagctggca aaccagattg ttgatgttaa agcactcaat aaaacagagc
    46321 tgcaaattac cctgaaaagc gcctactatc ctttcctgca agaactggcc ctgccccgtc
    46381 ctttccgttt tatcgctccc tcgcagttta aaaaccatga aaccatgaac ggaattaaag
    46441 cgccgattgg caccggaccg tggattttgc aggaatcgaa actaaatcag tacgatgtct
    46501 tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat taaaaagatc acctttaacg
    46561 tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac tggcgatatc gacctgctgt
    46621 acggaaacga agggttatta ccgctcgata ccttcgcccg ctttagccag aatccggctt
    46681 accacaccca actgtcacag ccgatcgaaa ccgtgatgct ggcgctcaat accgccaaag
    46741 cccccaccaa cgagctggca gtacgtgaag ctcttaatta cgcggtaaac aaaaaatcgc
    46801 tgattgataa cgcgttatat ggcacccagc aggtcgccga caccctgttt gccccttctg
    46861 tgccttacgc taatctcggc ctgaaaccgc gccagtacga tccgcagaaa gcgaaagagt
    46921 tgctggaaaa agccggttgg acgctgcctg cgggcaaaga catccgcgag aaaaatggtc
    46981 agccgctgcg cattgaactg tcgttcatcg gcaccgatgc gttaagcaaa tcgatggcgg
    47041 aaatcattca ggctgatatg cgccagattg gcgcagatgt ctcgctgatt ggcgaagaag
    47101 agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg catgattttc caccgcacct
    47161 ggggcgcgcc atacgatcca cacgccttcc tgagttcaat gcgcgtaccg tcgcacgctg
    47221 acttccaggc acagcaagga ttagccgaca agccgctgat tgataaagag atcggcgaag
    47281 tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta tcgcgacatt ctgacccgtc
    47341 tgcatgacga ggcggtttat ctgcctatca gctacatctc gatgatggtg gtatcaaaac
    47401 cggagctggg taatatcccc tacgcgccga tcgccaccga aattccgttc gaacagatta
    47461 aaccggtgaa accttaatgt tgcgttacgt attacgccgc tttctgctgc tgatcccgat
    47521 ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc ctcggtaccg gcgacccggc
    47581 gctcgattat ctgcgtttat ctaacctgcc gccgacgccg gagatgctgg cctctacccg
    47641 caccatgctc gggctggatc agccgctgta cgtccagtac ggtacctggt tgtggaaggc
    47701 gctgcatctt gactttggta tctcattcgc cagccaacgc ccggtactgg acgatatgct
    47761 gaacttcctg cccgccacgc tggaacttgc aggtgcggcg ctggtattaa ttctgctcac
    47821 ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat cgtttgccgg atttcgccgt
    47881 gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc tggctggcat ttttgctagt
    47941 gatggcgttt tcggtgtatc tgcaatggct acccgcgatg ggctacggca gctggcagca
    48001 catcatttta cctgcggttt ccatcgcctt tatgtcgctg gcgattaacg cgcgactgct
    48061 gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc acctgggcgc ggctacgcgg
    48121 cctgaatgac aaacagaccg aacgccgcca tattctgcgc aatgcctcgc tgccgatgat
    48181 caccgccgtg gggatgcaca ttggcgaatt gattggcggg acgatgatta tcgaaaacat
    48241 ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg atttttaacc gtgactatcc
    48301 ggtgattcag tgctttacgc tgatgatggt ggtggttttt gtggtctgta atttgattgt
    48361 cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt catgaaggag cgcacgcgtg
    48421 aactttttcc tctcttcccg ctggtcggta cgcctggcgc tgatcattat cgccctgctg
    48481 gcgctgattg cgctcaccag ccagtggtgg cagccgtacg atccacaggc aattgatttg
    48541 ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg gcaccgatca cttaggtcgc
    48601 gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc tcggttcggt aatggcctgc
    48661 ctgctgctgg tgctgacatt agggctggtt attggcggca gcgccgggtt gattggcggg
    48721 cacgttgatc aggccaccat gcgcgtcgcc gatatgttta tgaccttccc gacctcgatt
    48781 ctgtcgttct ttatggttgg cgtgctcggc accgggctga ccaacgtaat tatcgccatc
    48841 gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca gcttggtgat ttcactacgc
    48901 caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg gccatgtgcg ggtgtttgtc
    48961 gatcatctgg caggcgcggt gatcccttcg ctgctggtgc tggcaacgct ggatatcggc
    49021 catatgatgc tgcacgtcgc ggggatgtct ttccttggcc tcggtgtgac cgcgccgacc
    49081 gccgaatggg gcgtgatgat taacgacgcg cgccagtata tctggaccca gccgctgcaa
    49141 atgttctggc cggggctggc gctgtttatc agcgtgatgg cctttaacct ggtgggtgac
    49201 gcactgcgcg atcatctgga tcctcatctg gtgacggagc acgcacacta atgccgcaac
    49261 agattgaact acgtaatatc acgctacagg ccgcgcagcc gctggtacac ggtgtatcgt
    49321 taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg tagcggcagc gggaaatcat
    49381 taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt tcgccagacg gcgggggaaa
    49441 ttttagccga tggcaaaccg gtttcgcctt acgccctgcg cggcatcaaa attgccacca
    49501 tcatgcagaa cccgcgcagc gcctttaatc cactgcacac catgcacacc cacgcgcggg
    49561 aaacctgcct ggcgttaggg aaacccgccg atgacgctac gcttaccgct gccatagaag
    49621 cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta cccgttcgag atgagcggcg
    49681 gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg tgaatcaccg tttatcatcg
    49741 ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc gcgcatcctc gatctgctgg
    49801 aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt cacccatgat atgggcgttg
    49861 tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg taaaattgtc gaacagggcg
    49921 atgtagaaac gctgtttaac gcccccaaac atacggtgac gcgcagcctg gtttccgctc
    49981 atctcgccct ctacggtatg gagctggcat catgacctta cttaacgtct gcggcctttc
    50041 ccatcactat gcccacggtg gatttagcgg aaaacatcaa catcaggcgg tgctgaataa
    50101 cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg ctgggacgca gcggctgcgg
    50161 aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct ccctcacaag ggaatattag
    50221 ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa cgtaaagcct tccgccgtga
    50281 tattcagatg gtgtttcagg actccatcag tgccgtgaat ccgcgcaaaa ccgtgcgcga
    50341 gatcctgcgt gaaccgatgc gccacctgct atcactgaaa aaatccgaac aactggcgcg
    50401 cgccagcgaa atgctgaagg cggtcgatct cgatgacagc gttctcgaca aacgcccacc
    50461 gcagttaagc ggcggccagc tccagctcgt ctgcctggca cgcgcgctgg cggtcgaacc
    50521 gaaattgctg attctggatg aagccgtttc taaccttgat ctcgtgttac aggcgggcgt
    50581 cattcgcctg ctgaaaaagc tacaacaaca gtttggcact gcctgcctgt tcatcactca
    50641 tgacttacgc ctggtggaac gcttttgcca gcgagtgatg gtgatggaca acggacaaat
    50701 cgccgaaacc caggcggtgg gagataaatt aaccttttcc tctgacgccg gacgtgtgct
    50761 acaaaacgcg gtattacccg cattccccgt gcgccgtcgc acctcagaaa aggtttaacg
    50821 ccaatgcaac gagtcaccat cacgcttgat gacgatttac tggagacgct ggacagcctg
    50881 agccagcgtc gtggttataa caaccgttcc gaagctatcc gcgatattct gcgtagtgcc
    50941 ctggcgcagg aagctaccca gcagcacggc acacaaggtt tcgcggtgct gtcgtatgtg
    51001 tatgaacacg aaaaacgcga cttagccagc cgtattgttt ccacccagca tcatcaccac
    51061 gacctctccg tcgccacgct acatgtgcac atcaaccacg acgactgtct ggaaatcgcc
    51121 gtgttgaaag gtgacatggg tgacgtgcag cattttgccg atgacgttat cgcccagcgc
    51181 ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag attgagttat ttctaaaaca
    51241 aggaaagaaa agatattaac tgaacttaat ttataaaatt aagttcagtt gcatagatag
    51301 atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta aaatttataa atgattcaaa
    51361 cgctcattca gtgtttcaat aatataggta ttaacagaga cttgttgctg cgcagccgca
    51421 ttattaaggc gttcactcag cgattctgga tagcgtaacg taaaggtctt aatcttttct
    51481 gtacgcgcat acggctcaat acccgcagct ttacaatcct caagatactc tcgcagggaa
    51541 agctctcctt ctttttgcag cccctgaata ctgtcagaga caaaatcaca ataacctgac
    51601 aagcccagga atttgccacg aaatgcattc agttctggca cgtaggtaat aacagctggt
    51661 tgcccggcaa tctccataga gtttggcgtc ttcagtttga tcatggtttt acccctatac
    51721 ttagtaacca gtcacacacg ctttctacag cgcctttatc ggtatctggc gacggatgag
    51781 gccgatggaa acacgcaacg ctcatattta gtatgaactt gcaacgcgaa cctcttcctt
    51841 ccttaatttc tccgcctaat gctttaacca gtgactctat atcagaccac ttgatccctt
    51901 gaggaacagg agttttaaat atttgatcca atgtattttt ttgtttcttc cgtaaagcct
    51961 gtaccttggt tctcatggca tgtccttatg acttcacttt atgacatcac agtatgaagt
    52021 caagtttccc ttccttacgc cattgtccca atcgtcttcc tgaatcgcag cagtgcgatg
    52081 gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact gcggccagac gatttcgaat
    52141 ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa agtgtgtcgt cggcatggtc
    52201 agcataatgt cctgcaccat ctgcggcata ctttcgcgcg gcgtggatcc accggaaagc
    52261 atttgcagcg gcagcagcac cagaatcacc agcagcccca gttgcggcat tgaacgcgct
    52321 atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca gactgagcgc cacgcccagc
    52381 ataaacagcg ggatcgagcc ttcaatcggt acgcccagta cacctttcac catcagcacc
    52441 agcgataatc ccgataccac cagcaccacc agccccatcg accagatctt cgccatcatg
    52501 atctcaaacg gcgttatcgg catcaccagt aaatgttcca ccgtgccgtg ttcacgctcg
    52561 cggatcagcg ccgatccggt caatacaatc gccagcatgg taatgttgtt gatgatcgcc
    52621 atcaccccgc caaaccacgc gggatcaagg ttcgggttaa agcgcatccg ggtttccagc
    52681 gataccaacg gttcgctgtt atcacggtag cgcgcgacaa agctgttcac ttcaccgttg
    52741 ataatattct ggatataccc attgccggta aatgcctggc tcatgcgcgt ggcatcgacg
    52801 ttcacctgaa tatccggctg gcgtccggcg aggacatcac gctgaaaatt aggcggaata
    52861 tttatcgcga aggtatagcg tccggcgtcc agtccggcat ccatctcatc ggcggtgatc
    52921 atctccggtg gcaaaaacca cggacgatag aagctgttaa cgatccggtt cgataactgc
    52981 gattgatcca tatcggcaat ggcgatcggc gcgaggttca acgatcctgg cgtaacggtc
    53041 gctgacgaat acaccgacac cgtaaacgag aagacaatca gcgtcagcat cgctttatca
    53101 ccgagcagac tgcgcaactc tttgataccc agattaaaaa tattgcgtaa atggcgcatc
    53161 atccctcctg ttttttcagc agcaggatgc ttaagcccat caccagcggg atggctatca
    53221 gcaacgggat aaaaagttgc cacaaatcag tcagatccag cgctttcgag aacgttccgc
    53281 gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc gatccaacgt ccaggccctt
    53341 ccagcgaagc taccggatcg atcatcccgg aaaactgtgt cgccgggatc aatgtgataa
    53401 tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa ggtggagatc agcagcccca
    53461 ttccagtggc aatgatgata tacagcagcg ccgccaaggt gagcgtcagg aaactgcctt
    53521 tatgcggtac gccaaacaca aacaccgaca ggccgcagag caggaaaaag ttcagcatcc
    53581 ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc gctacgggtg gtgggggtca
    53641 cgtaaaggtt gataatcgac cctagctctt tttcccgcac cacgctaagg gcgcttagca
    53701 ttgacgggat catcatcagc agaagcggga tcaccgccgg aacaatcgct ggcaggcttt
    53761 ttacgtccgg gttatagcga taacgcgtct caatgttcat cagcccgctt tggctggcgg
    53821 gtgtcgattg tcggctcgcc acatcctgaa gccaactctg gtgcattgcc tgcacgtaac
    53881 cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat ccagacgccg agttccacag
    53941 gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat ctcaatggcc accgtgatat
    54001 cgcccgcacg catccgacga tcaagctcgt cataactggt gagcggcggc tgttcgataa
    54061 agtaacggga accggagagg ttgagtgtcc acgcctggct actgacggtc tggtcgcggt
    54121 cgagcaccgc aaagcgcagg ttttccacat ccatactgat gccgtaaccc attatcagca
    54181 tcaggatcac cgttcccatc agcgccagcg tcgaacgtac tggatcgcgt cgcagttcca
    54241 gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct aaatccctga cgcggcgcgt
    54301 gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt gctctgccct gccgcttcct
    54361 gcaaataggc gataaatgcc tcttccagac tggcggctcc gcgtttctca accagttcct
    54421 gcggtgtacc gctggcaagc acttttccgg cgtgcatcag tgagatgcgg tcgcaacgtt
    54481 ccgcttcgtt cataaagtgg gtggagatga agatagtcac tttgtcctgg cgcgagagat
    54541 cgaccatcaa ctgccagaac atatccctcg ccaccggatc gacaccagaa gtaggctcat
    54601 cgaggattaa catctccggg cgatgaatca ccgccaccgc cagcgaaagc cgctggcgaa
    54661 tgccgagcgg caatgactcc ggcagaacat cttcaacgtc gttgagctta aaacgctcgc
    54721 tcatttcagc cactcgcgcg ggaatttccg cttccgggat gtgaaacaaa cgggcatgta
    54781 actccaggtt ttgccgcacg gtgagttcgt tatagagcga aaacgcctgc gacatatagc
    54841 ccacacgacg gcgggtatcg atatcttttg gatcaaccgg ttgcccaaat agccacgcct
    54901 caccttcgct ggcgggcagc agtccggtaa gcattttcat ggtggtggat ttaccgcagc
    54961 cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat gcggaaattg acgtgatcaa
    55021 cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc ttcgatggca atctctgcgt
    55081 tttcaggttg atacggtggg atcactaccg cctgatgcgc ctggcgttgc gcttgcggta
    55141 acagattaat aaatgcttct tccagcgtgg cgctttgcgt ttgctgccgc agctcttcgg
    55201 cgctgccggt tgccagcact tctccggcat tcatcgctac cagccagtcg aagcgttcgg
    55261 cctcttccat ataggcggtg gcgaccagca cgctcatatt gctctgccgc tggcgaatac
    55321 tgtcgatcag atcccagaac tgggcgcggg agagcgggtc aacccccgtt gttggctcat
    55381 caaggatcaa cagttccggg tcgtggatta acgcgcagca cagcccaagt ttttgcttca
    55441 tcccgccgga gagtttacct gccggacgat cgcgaaacgg tgctaacccg gtgctggtca
    55501 gcagctcatt aattcgcgct tcccgctccg ctttatcgtg accgaacagg cgagcgaaaa
    55561 aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt tttgcccagc ccctgcggca
    55621 tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg gtcgtgcata tcgccaccca
    55681 gcaccaccac attgccctgc tcaatgacgc gggcaccgga aatcagtgac aataaactcg
    55741 acttcccgac gccatccgga ccaatcagcc cgaccatgca acgggcctga atatcgagag
    55801 tgatattgtt cagcgcaacg gtttttccat aatgctggct cacgcccgcc agttgcgcga
    55861 caggcgggac gggaaccagt tccagatgcg tcattgcggc aacctcacca cgaggtcgtc
    55921 aggccacgga agttcttcat tcacccgcac ccacgctacg cccggtaaac cggttttgac
    55981 atattccaga tgctgctgga gtaattccgg tgggatacgc gctttgacgc ggaacatcag
    56041 tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg aactgggcga cactggcgac
    56101 aaaactgatg gttgcaggaa tacgcagatc tggcgcggca tcgaggatca gccgggcttc
    56161 accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg aagaaggtca tatagacgtc
    56221 gctgagatcg accatattca gcacccgacc gcctgccgcc agcacttccc ctggctcggc
    56281 aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt tcactgtcat cgatatctgc
    56341 ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga gtttgcgcct gaatgatatt
    56401 ggtgcgtgct gcttctatag ccgctttaga agccgaaacc tgggctttcg ctgattccag
    56461 cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc agctgttgcg cagaaatagc
    56521 ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg gcaacagagt ccagttcggc
    56581 ctggcgttga ttgaccagcg actgtgcggc acgagtttca ctttgtcgtt gctccagcaa
    56641 agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt tgcgcgatgg cttccagtcg
    56701 ctgttcctgc aacacgcgag tatccatctt cgccagcact tcgccttcgc gaacaaactg
    56761 gccttctttc accagaatgg tgtcgatacg cccggcaatt ttgctggcga tatccacttc
    56821 cgtcgcttca atgcgcccat tgctgacagc aaagccttcc ggcacacccg ccgggcgcaa
    56881 cagccaccac gccacgacgg cagccaccgc cagtgccccg acaacccacc acgccagatg
    56941 gcgcttactc ttatccataa tcgacccgcc ataatccctg taagcaaacg acgcgtgtta
    57001 caactgccgc cgaacgacgt tcagcggatt cttccatccg ggataagaag gaaacgacaa
    57061 atacgaccag cgcgcagagc attgagcgcc aacggggaag gcttagtgaa aacgggtagt
    57121 aatgttgtca tcatcagcat tcctggccgt aaatgaaaca cgcatactca taagtgtgat
    57181 ctataaaact ggctgacaaa acggtatcca tcctgttgga tcgcatttca cgcctgttca
    57241 gttgcaggaa cagagcattc ctttttacgt tttatcaaca gtctcaacca gatagtaaca
    57301 ttaatattca tgttgtctat ggttcagagc tgtaacattg tgcgccttct aatcaaaggc
    57361 ataagtccat ttctgttttt ttcatgctgg taagacttcc agggagtggt tatctctggc
    57421 cctgcaccag ggttaatttg gcaattatgc atgctgaaca ttcttccagc atgtttactg
    57481 tcacgggcgt taagacctaa aactgtcccc gtttcaccta tgcgttctct cccaccattt
    57541 ccgttatgtg caacaacttg atttatacct aacatttgtg aacatgctaa tagtgcttca
    57601 agtttaggtc tgtaatctgt cgaaacagaa aaataacgat taaaggatcg acaagagata
    57661 gcccggctag ttttaaactt tttgccatta attatagcat gatgcttttt attaataatt
    57721 tccgctaatt cgaccggatc gagagcattg gtcatttcat caatagagac agttatcggt
    57781 cctataatgt aacgttttcg ctgatcatca aaagttatcc catgatgatt tgccattatt
    57841 ttgtatttgg ggtcatagaa acatacagga taatctttta tcccgtcgta ggtatcctca
    57901 tcaagtggtt tcatgcgcgt gaagttttgc atatagttac cattgcaatt cgtttcatga
    57961 tttccagcta atacatgaac attttcattg atactcatta tgcgttttaa caaatcgatg
    58021 ataaattgat cgccacagat attactaaat cgatcgccgg tttggtcgcc aagaaacgta
    58081 catggcgtga ctgcgtcttt attaataacc aaataaggct gaagactttc taaaacatta
    58141 tctcttctgt tcagtatact gattattgta ttaacattag tgtcaagttg ctcattaaag
    58201 ttctccctta gcaatacgtt gagaacttca tcttctatat taagcaactg gctaaggaaa
    58261 ttttctcctt cagccatgat tcccaaatgt cctgttgcaa aaagcgcgta caaaactgca
    58321 ccgactgagc catcggtatc gccaaaatag gtcggcctgg tacttgtggg atcaacaggt
    58381 gaacaattgg agaaataggt tctgtcagcg aactggggtt ttataattgt ttcgaattta
    58441 atatcaaata cattgtattg cgatatttcc cagttccaga tgggtttgta cgactcagta
    58501 actgcaacat cattagcatt atcatttact gtcatattat taagtaaata aacaatgctg
    58561 caccaattaa tatcggtatt atcaagaggg caaccgtaat ttttactatt aattgtcaca
    58621 ataaactgat aacagttatt cctacgtaga atatgagcgc gcatgcccga tagcatacta
    58681 tatttttctg aatgattcgt ttgtatggta aatgaaattt tgtcatcttg aatagattgt
    58741 tggaaacgtt ctatttgcca atcatcaatt ttttccacaa cttcaccgag cattgtcctg
    58801 gcaatacttg atatatcatc acctatacca tttgcaatat acccaaacag ttctttagtg
    58861 aagttggtaa catgctcttg caccatatca tttgttgcga ttgtcgttgt actgccatta
    58921 gtgcctgcca cagttccaat tttcatcttt tacctcatat tattcaccgc gaggaataca
    58981 tagttaacat tttatcttat gacatacacc tcatattcga tggggaaata attatatttt
    59041 gcacaaatca ttcagcctta aagcataata tgaaatttct tatctcatag cagagaaagt
    59101 ccctaaatat tgtagaggcg tcatcagtac atacctctac aatcgattaa gaaaacctat
    59161 gaaatactgg caaaattaaa aagagatacc acagcactca tgaagtttta ttgaggttga
    59221 aaacaactaa gccctgggac acctttttta tcaccacgtt cgttaggtgc tatcttccaa
    59281 gaatttagcc ccatggtata attatcaatt ttttgatgct ggtctacccc taatccataa
    59341 tggccaaatt tctgtttagt ttttttgagc gtctgtccac atttgaagat atcttcaggt
    59401 tttggtctga aagcagtagt gttattaaaa taattttcat atacaggtgt gggtgctata
    59461 gcattgcttc taattagatg gagaagatga tcatccatgt gttgtttatg ttttttatta
    59521 catatatttg ctaattcctc aggatcaatt gggtctttca tctgattaaa tggaacctgc
    59581 aaagcaccga gacaataaca ttgattttct tcatttctta ttattccgtg atgactggtt
    59641 aaaactttag tttctgagtt ataattacaa acatcgagcg ttttaattaa gttatacgta
    59701 tcaccggcag agagtttata attttctagt cgtgcagtat agttaccatt aaaattgatt
    59761 tcatgatttc cagccagaac gatgacattt ttattaatac ggctatcttt attaccctcc
    59821 atatttcgca tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat
    59881 ctatcgcctg tatgatcccc aaggaagata caaggggtta tcgcatcgtc ctttaaaaaa
    59941 acatgttgat gcaactcatt caatatcatg tctctcgtat ttaataaatt gtagagcaat
    60001 ttattatttt tatgtatgta tgcagatgaa gccatgtctt cgtgttttaa taattcacat
    60061 aataaactcc aacccttttc cctgatgcct atatgccctg aaacgagcaa tgaataaagt
    60121 acagcgccta ctcttccatc tgtatcgcca aaataagccg tgttggcatt ttgtgggtta
    60181 atcacaccac attgtttata aaagtctatt ttagaaaaca cagatttaac atagctatca
    60241 aacttatcct taggaaacgt ctctttagag acatcccaac gccagtcagc tttaaaatta
    60301 ccgctaagaa gagtaaagaa attttcaggt atgatagaaa gcccattaac tttataaatt
    60361 atgtctgatt tatctccgga gaaattaata aaataataac catccttaca ctcaatctta
    60421 actgtaatgg gagacgataa ttttatcaca tggttattgt ctggtataat tgtaaagata
    60481 aacgtgttag tagtattatc ggcaaaacta ttatattctt gaatatctat agatggctca
    60541 ctattgtaaa accatatttt ctccatctga agactatgtt catttaccct ccagtgacca
    60601 tgttgcagga aatagatgat atgcatgcca aaatcgttat ctatttgcga atcattatcc
    60661 agcttatcaa tttcaactat ctttgaacga tgtgttttat caaccttgct atggtgttga
    60721 tgattattaa ctttatctga atgcttatct ttatggtttg aagggatgta cttaaatata
    60781 ttgtcaattg ctttcataaa taacccgcga tatttttgac ctgttagtaa aaaataaatg
    60841 atacatgtct gaaagacata tttacgacga tataaaacaa taatacttat atgttcgctg
    60901 attttaagca ccgagactat ccggtctttt actaaataat ggaaaacaat ccatccctgg
    60961 aagacaattg taaaaaaaaa caacagaata agaattaatc ttaggataaa tatttattta
    61021 tcatggcctt ttgaccgcta aaaaatggca ctcggcacag taaaccccgc tatagttatg
    61081 ctatgcattt gtctttaatg atatctcgtc gttatattaa caggctgaaa ttatcattaa
    61141 tgcacatttc aatattagca gggatacctg aggagtttaa agtgaacata tatatcgggt
    61201 agcttttcaa attaatccct ttgattatag gcttaatttg catcgcgtta ggtggctttg
    61261 tgctggaaag ttcagggcaa cgtcacgggt catgtgctga tttctctggc ggccatatgc
    61321 ctggcattat tcactaccgc atttattatc atttcgcaac tcacgcgcgg cgttaatacg
    61381 ttttacaata cattgttccc cattattggc tatgcggggt caattatcac catgatatgg
    61441 ggttgggcac tgttagcagg taatgatgtg atggcagacg agtttgttgc cggccatgtt
    61501 atttttggcg ttggtatgat tgccgcatgt gtatcgacgg tggcagcgtc gtccggtcac
    61561 tttctgctca ttcccaaaaa tgcagcgggg agcaagagcg acggaacacc ggtacaggct
    61621 tattcttcat taatcggtaa ttgcctcatt gccgttcccg ttttactcac cctgctcggt
    61681 ttcatttggt ctattacgct gttacatagt gccgacataa ctccgcatta tgtcgccttg
    61741 ggttaaccgc aatctgtgcc tgtctaattg gcctagtggc cacaatcgtc catcaaacac
    61801 gtaatacgtt ctcatccaaa gagcactggc tgtggtgtta ttgggttatt tttctcggct
    61861 cagtcacggt gctgcagggg atatacgtct tagtcagttc cgatgcaagc gcccgactgg
    61921 ctcccggcat tattctcatt tgcctcggaa tgatctgtta cagcatattc tcaaaagtct
    61981 ggctactggc actggtctgg agacgtacct gttcgttagc caacagaata ccgatgatac
    62041 cggtatttac ctgtctgttt tgcttgtttt tgtcatcatt cctcgcggaa atcgcacaaa
    62101 ctgagctgga ctactttatt ccatctcggg ttctggttgg attaggcgca gtatgtttta
    62161 cattgttctc tattgtttca atattagagg ccggttctgc gaaaaaaaat taaaatttac
    62221 ataatcccag cgttattcat atcttaacgc tggtttttta cgaacttagt gaaataatgt
    62281 agaatataaa attataattt tatattggag cacatgtgtt aatgatcaac cccgggatac
    62341 taaaaatata gacattatta aacttgctta gcctcgcatt gcctttacct gttaaagatg
    62401 catcgagaaa attgagtctg aagttctcat cctttatctg attttccagt cccgaaactt
    62461 tggccttagc attacctgag atccgtccgt tactatctat tttgtaggta aaaaaatacg
    62521 aatgggaaaa tttcctcggt gtatcttcat cagttaagcc aacaatcgta aaactaccgc
    62581 taccatcttt ttttaaaatg actgaaaggt tagcagagta gtataaagaa tgatctgttt
    62641 tagcatttct tgttaacgtc gacgtacatt caagagacgt tgtgctacca tcacgagcct
    62701 tccagagagc aaaaaaacac acctgtagca atgtgaaaac aagtattaat ataaaataca
    62761 ttttcttatt agtcatataa tagcacctct gaacaaattg cataagtttc gtcattagag
    62821 gattttatac atcttgataa ataccgctgc ccttccccaa gcaaataatg catactattt
    62881 acatcatata taaaatttgc acctggcaca cagggtaaat gctttttcga aatttcagcg
    62941 gcctctattt gagatttagc gatgccaatc tcgttaagat tctctgaaag atataaatca
    63001 caggtgtcaa tcttacctga tttaaccaat ggataatccg ttatactgta ggtatcttta
    63061 aaagccacgg caaacatttc aataataaca attactacca caagcgaaat caaaatgcga
    63121 aaaagaggaa tactcctttt gcgctctact ttttcaacaa catcttcttg taaatgagaa
    63181 tcatcataag attgtttact tattgttatg ttttcactga tgtaaaagcc tactttgggt
    63241 atcgtaagaa tcacattttc ttcaacacca atatttttaa aacttttgcg taacagggat
    63301 atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa atctctccaa aacgctatca
    63361 atgattgagt tcctgtcaac cgctccacca ttactttcca gtaacactgc caaaatacac
    63421 cccgccgtag ctggaaaatt tatctcatcc tgggtattta cattcgagag cgagcatttg
    63481 ttcggatcaa aaataatcgc gtcattgata atgaaaaaac atctccgctc catgcaggca
    63541 acaacacaaa ataagatttt gtcattttag cctattcctg gtggaactga cacaagaaac
    63601 taaaatacaa atataaccct gaaaaagtca tataaaactt atatccacag aataaaaaat
    63661 caaacatgtc agttttatca atataaagta tcgcaaggcg aaaagtgatg caagaatact
    63721 cctaagtaaa tgaaagcgca ccacatgaaa ttatcaacac atgtaatttc atcattaggt
    63781 gctatcgaaa ataactcagg agctacttaa gatgaataat gtcttactca tgagacccga
    63841 tgactcagaa tttatgatac ctgatgactg gtcagggcta aacgcaaaag gcattacata
    63901 cgaacaagat aatagagtga cagagtcttt tctgcctttt aaatcttttt attgttcacc
    63961 ggtgtgcacg acagttgaac tcatctcata aaataattgt tttgctatgg gtatccgcta
    64021 ccttctggaa aagaaaagag acttaaggct aaatcacttt aaagccttct ctcataaatg
    64081 cgagacaaaa cctgacgtgt taattatgac cgggtgcacc gtcgctgata cttatagtat
    64141 attctcacgc attgttgaag aaaatataat gaaaacgatg acacctatcg tcatcattac
    64201 tgggcatcac tcagaagatc tttgttctct ggcatcgaaa ctgggaggag ttaaaataat
    64261 atcaccgaca gaaaagattg caaaaataga aaacgctatc aatgatgctt ttaacagcaa
    64321 gcctaaaaaa ataagatttt taacagatat actctctcct caacaaattg agaccatgaa
    64381 aatggttgcg cagggtagat ccgttaatga gatttcagcc gcaatgaata ttagcatcaa
    64441 gaccgtgtat gcacataaag ttcgtgctat caataagctg ggaattaaga aaaaagttta
    64501 tgaagcgctt ttttataaag cgctaattca gttcaactga tgtaaaaatc accctcgctt
    64561 aataaataat atattctatg tcttttttta taagttaaaa aaggcgggat atcaaatccc
    64621 gccatgaaat taactaatca tctgcattat tctgcgtaga tataagcctg ggtacggatg
    64681 gtgtaagagt ctggctcaac aacctgttct ttcatatggg aaacgacgcg ataggcacca
    64741 cctgttccag tatttttcag catcatataa cgcttaccgc caatctcttc ggaggcagct
    64801 gaaggaggat cctgctcaag cgttgtcagg gaaactttca gtttatggtc cgggttattg
    64861 tcaccagaaa ttactgcatc cgctgaagta ctgctctccg cataaatatc atccgggaaa
    64921 gttacagcat agtcgtggct ctgcccatca gatgcgccaa caatacctgt tgccaaaggc
    64981 gtccatgcag ttaacgtacc gctatatacc cctttcactg gttgaatggt attattaaca
    65041 acgataggct tggtcaaagt aagattagct gacgcttcag taccctggct tactaaagtt
    65101 cccgcctgag aagcaaaagc tgtccccgcc agcatgattg ctaacaatgt tttcttcatt
    65161 atattttctc catgattttc atttacactg caaaatgcag tttctttctt tgcatttacg
    65221 tgctatcgcg aaaatgcttc caccaaataa tggaaacatc ttcacaaaaa taaattatga
    65281 gtttcttaat ttagaaacta taaacatgtc gccgctatct ggtataaccc tgatgcactt
    65341 ttcttcgcag gaagttggta ttttgcgata caggattgat ctttagaata accccatttc
    65401 acattaaact cgccttccgc actcaagcca gaaacataag caataccatc ttcacctaca
    65461 atgccggcca tattttcagt gtctttgcta taactaatca cagcgccgaa ggggatgggt
    65521 ttaccctctt tctgttttaa ggttactaat cctttatccc cgctcttcac tgccaatgtc
    65581 gcaggcacaa cggctccagc tgtcggtaca accaattgac tagtttgtgg taagtcgtaa
    65641 ttcttaccta tcgttttttc atccagaata acatcgtctc ttcggtatgg ctgcactggc
    65701 tgtattgcat acccacgaaa atcagtactc agtcccggcc agccattaac gttaaccccc
    65761 gttgctccgg gtgcctctat aagggctaat gtcccatcca cattttgacc aaatgtaagt
    65821 ccatcccttg taataagtat cccaccgctt aaatttgcac ctagctgttt catgttttcg
    65881 ctggagctgt aatggctact aaactgacca tatgatccat ttttgccaac ttgcacaatg
    65941 ttcatcgtgc tcttcggttg tgactgatga gcgttagtta tatttgtcgc tacatcccac
    66001 catacaccac cactattctg acctgataat gaaacctgat agttattacc ctgctctccc
    66061 gaacgactca tctgtgaaga cgcataaatt tgattttcgt cacccagcga taatggcact
    66121 gaaataccca cgcttaattt ccgatctact ttccatttat gcgatgtcag caagtcgttt
    66181 tttgcccagt taatggagaa tgttatgcct ctccataccg tactataact caggctggta
    66241 gaccgcgtgt ttcctgtgcc ccgggaagaa ttatacctcg tggtattgaa gttcaaattc
    66301 ccgatattac caaggggctg ggataaggat atcgtagagc catcg
//
LOCUS       DAJTRM010000009        66032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRM010000009 DAJTRM010000000
VERSION     DAJTRM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.009"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO322_000638"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO322_000638"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5905634.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO322_000639"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO322_000639"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5905635.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO322_000640"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO322_000640"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5905636.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO322_000641"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO322_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5905637.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO322_000642"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO322_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5905638.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO322_000643"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO322_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5905639.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO322_000644"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO322_000644"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5905640.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO322_000645"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO322_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5905641.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO322_000646"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO322_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5905642.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO322_000647"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO322_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5905643.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO322_000648"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO322_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5905644.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO322_000649"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO322_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5905645.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO322_000650"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO322_000650"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5905646.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO322_000651"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO322_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5905647.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO322_000652"
     CDS             19304..19369
                     /locus_tag="OO322_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905648.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO322_000653"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO322_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5905649.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO322_000654"
     CDS             complement(19753..20376)
                     /locus_tag="OO322_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5905650.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO322_000655"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO322_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5905651.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO322_000656"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO322_000656"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5905652.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO322_000657"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO322_000657"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5905653.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO322_000658"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO322_000658"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5905654.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO322_000659"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO322_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO322_000660"
     CDS             complement(26460..26846)
                     /locus_tag="OO322_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5905655.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO322_000661"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO322_000661"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5905656.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO322_000662"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO322_000662"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5905657.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO322_000663"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO322_000663"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5905658.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO322_000664"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO322_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5905659.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO322_000665"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO322_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5905660.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO322_000666"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO322_000666"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5905661.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO322_000667"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO322_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5905662.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO322_000668"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO322_000668"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5905663.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO322_000669"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO322_000669"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5905664.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO322_000670"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO322_000670"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5905665.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO322_000671"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO322_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5905666.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO322_000672"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO322_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5905667.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO322_000673"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO322_000673"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5905668.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO322_000674"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO322_000674"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5905669.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO322_000675"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO322_000675"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5905670.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO322_000676"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO322_000676"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5905671.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO322_000677"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO322_000677"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5905672.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO322_000678"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO322_000678"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5905673.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO322_000679"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO322_000679"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5905674.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO322_000680"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO322_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5905675.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO322_000681"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO322_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5905676.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO322_000682"
     CDS             53914..54303
                     /locus_tag="OO322_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5905677.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO322_000683"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO322_000683"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5905678.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO322_000684"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO322_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5905679.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO322_000685"
     CDS             complement(55962..56162)
                     /locus_tag="OO322_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5905680.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO322_000686"
     CDS             56328..56873
                     /locus_tag="OO322_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5905681.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO322_000687"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO322_000687"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5905682.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO322_000688"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO322_000688"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5905683.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO322_000689"
     CDS             complement(58171..58995)
                     /locus_tag="OO322_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5905684.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO322_000690"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO322_000690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5905685.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO322_000691"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO322_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5905686.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO322_000692"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO322_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5905687.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO322_000693"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO322_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5905688.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO322_000694"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO322_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5905689.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO322_000695"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO322_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5905690.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO322_000696"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO322_000696"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5905691.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66032
                     /locus_tag="OO322_000697"
     rRNA            65762..>66032
                     /locus_tag="OO322_000697"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15627 a        16347 c        18196 g        15862 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccga agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DAJTRM010000010        64037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRM010000010 DAJTRM010000000
VERSION     DAJTRM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.010"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            133..576
                     /locus_tag="OO322_000698"
     CDS             133..576
                     /locus_tag="OO322_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5905692.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="OO322_000699"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="OO322_000699"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5905693.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO322_000700"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO322_000700"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5905694.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO322_000701"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO322_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5905695.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO322_000702"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO322_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5905696.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO322_000703"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO322_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5905697.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OO322_000704"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OO322_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5905698.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OO322_000705"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OO322_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5905699.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OO322_000706"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OO322_000706"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5905700.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OO322_000707"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OO322_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5905701.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OO322_000708"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OO322_000708"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5905702.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OO322_000709"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OO322_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5905703.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OO322_000710"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OO322_000710"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5905704.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDVRYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO322_000711"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO322_000711"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5905705.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="OO322_000712"
     CDS             13936..14316
                     /locus_tag="OO322_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5905706.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OO322_000713"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OO322_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5905707.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO322_000714"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO322_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5905708.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO322_000715"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO322_000715"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5905709.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="OO322_000716"
     tRNA            complement(17417..17492)
                     /locus_tag="OO322_000716"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OO322_000717"
     tRNA            complement(17496..17571)
                     /locus_tag="OO322_000717"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OO322_000718"
     tRNA            complement(17623..17698)
                     /locus_tag="OO322_000718"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OO322_000719"
     tRNA            complement(17701..17776)
                     /locus_tag="OO322_000719"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="OO322_000720"
     tRNA            complement(17912..17987)
                     /locus_tag="OO322_000720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="OO322_000721"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="OO322_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5905710.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="OO322_000722"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="OO322_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5905711.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="OO322_000723"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="OO322_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5905712.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="OO322_000724"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="OO322_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5905713.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="OO322_000725"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="OO322_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5905714.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="OO322_000726"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="OO322_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5905715.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="OO322_000727"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="OO322_000727"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5905716.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="OO322_000728"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="OO322_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5905717.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="OO322_000729"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="OO322_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5905718.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="OO322_000730"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="OO322_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5905719.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="OO322_000731"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="OO322_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5905720.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27908..30541)
                     /gene="mngB"
                     /locus_tag="OO322_000732"
     CDS             complement(27908..30541)
                     /gene="mngB"
                     /locus_tag="OO322_000732"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5905721.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30559..32475)
                     /gene="mngA"
                     /locus_tag="OO322_000733"
     CDS             complement(30559..32475)
                     /gene="mngA"
                     /locus_tag="OO322_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5905722.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32645..33367
                     /gene="mngR"
                     /locus_tag="OO322_000734"
     CDS             32645..33367
                     /gene="mngR"
                     /locus_tag="OO322_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5905723.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33470..34339)
                     /gene="sucD"
                     /locus_tag="OO322_000735"
     CDS             complement(33470..34339)
                     /gene="sucD"
                     /locus_tag="OO322_000735"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5905724.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34339..35505)
                     /gene="sucC"
                     /locus_tag="OO322_000736"
     CDS             complement(34339..35505)
                     /gene="sucC"
                     /locus_tag="OO322_000736"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5905725.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35599..36816)
                     /gene="odhB"
                     /locus_tag="OO322_000737"
     CDS             complement(35599..36816)
                     /gene="odhB"
                     /locus_tag="OO322_000737"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5905726.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36831..39632)
                     /gene="sucA"
                     /locus_tag="OO322_000738"
     CDS             complement(36831..39632)
                     /gene="sucA"
                     /locus_tag="OO322_000738"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5905727.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39933..40649)
                     /gene="sdhB"
                     /locus_tag="OO322_000739"
     CDS             complement(39933..40649)
                     /gene="sdhB"
                     /locus_tag="OO322_000739"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5905728.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40665..42431)
                     /gene="sdhA"
                     /locus_tag="OO322_000740"
     CDS             complement(40665..42431)
                     /gene="sdhA"
                     /locus_tag="OO322_000740"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5905729.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42431..42778)
                     /gene="sdhD"
                     /locus_tag="OO322_000741"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42431..42778)
                     /gene="sdhD"
                     /locus_tag="OO322_000741"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5905730.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42772..43176)
                     /gene="sdhC"
                     /locus_tag="OO322_000742"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42772..43176)
                     /gene="sdhC"
                     /locus_tag="OO322_000742"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5905731.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43557..43664
                     /locus_tag="OO322_000743"
     CDS             43557..43664
                     /locus_tag="OO322_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905732.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43870..45153
                     /gene="gltA"
                     /locus_tag="OO322_000744"
     CDS             43870..45153
                     /gene="gltA"
                     /locus_tag="OO322_000744"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5905733.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45544..46110
                     /locus_tag="OO322_000745"
     CDS             45544..46110
                     /locus_tag="OO322_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5905734.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46161..48620
                     /locus_tag="OO322_000746"
     CDS             46161..48620
                     /locus_tag="OO322_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5905735.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48635..49366
                     /locus_tag="OO322_000747"
     CDS             48635..49366
                     /locus_tag="OO322_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5905736.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49363..50439
                     /locus_tag="OO322_000748"
                     /pseudo
     CDS             49363..50439
                     /locus_tag="OO322_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50591..51637
                     /locus_tag="OO322_000749"
     CDS             50591..51637
                     /locus_tag="OO322_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5905737.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51634..52425)
                     /gene="nei"
                     /locus_tag="OO322_000750"
     CDS             complement(51634..52425)
                     /gene="nei"
                     /locus_tag="OO322_000750"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5905738.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52461..53195)
                     /gene="pxpA"
                     /locus_tag="OO322_000751"
     CDS             complement(52461..53195)
                     /gene="pxpA"
                     /locus_tag="OO322_000751"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5905739.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53185..54117)
                     /gene="pxpC"
                     /locus_tag="OO322_000752"
                     /pseudo
     CDS             complement(53185..54117)
                     /gene="pxpC"
                     /locus_tag="OO322_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54111..54767)
                     /gene="pxpB"
                     /locus_tag="OO322_000753"
     CDS             complement(54111..54767)
                     /gene="pxpB"
                     /locus_tag="OO322_000753"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5905740.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54790..55533)
                     /gene="ybgI"
                     /locus_tag="OO322_000754"
     CDS             complement(54790..55533)
                     /gene="ybgI"
                     /locus_tag="OO322_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5905741.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55804..57285
                     /gene="dtpD"
                     /locus_tag="OO322_000755"
     CDS             55804..57285
                     /gene="dtpD"
                     /locus_tag="OO322_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5905742.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57435..58853)
                     /gene="phrB"
                     /locus_tag="OO322_000756"
     CDS             complement(57435..58853)
                     /gene="phrB"
                     /locus_tag="OO322_000756"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5905743.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58850..59359)
                     /locus_tag="OO322_000757"
     CDS             complement(58850..59359)
                     /locus_tag="OO322_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5905744.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59488..59775
                     /locus_tag="OO322_000758"
     CDS             59488..59775
                     /locus_tag="OO322_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5905745.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59830..60210)
                     /locus_tag="OO322_000759"
     CDS             complement(59830..60210)
                     /locus_tag="OO322_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905746.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60213..63599)
                     /locus_tag="OO322_000760"
     CDS             complement(60213..63599)
                     /locus_tag="OO322_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5905747.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63618..63821)
                     /locus_tag="OO322_000761"
                     /pseudo
     CDS             complement(63618..63821)
                     /locus_tag="OO322_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15627 a        17249 c        15605 g        15556 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatgt gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caagacttat acattgataa atatcaattt ctacaaatta
    27421 tgttaatcat gtttcaacaa ggtaacttta gtgatattta tgtttaaaat gtgtgaatta
    27481 taagactgaa attagtttcg gttggcaatt aaagataaaa agagacgaag caaaaacagc
    27541 acggttgacc ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta
    27601 ttaataaagg cataaatctc cctcccgccg atattaacat ttaattaact tataattata
    27661 ttataattaa ctcacccgtg gttattattt tgttaaaaat aaagccacat atacgtagct
    27721 tgagagtgaa gataaacaca aaattacctg atgcgctacg cttgccagtg tctacatcat
    27781 ctctgcaata tattgaattt gcctgctttt gtagaccgaa taaagcgttc atgccgtatc
    27841 cggcatgaac aaagcgccct ttgtcaacaa tctggccgcg catatgtggc ttcggtttga
    27901 ttgctattca ggcaagccgg taactgaacg tccgtgactg gccgggtaaa aaaggccccg
    27961 atagatttga accttgattt tcctcggtgg taatgtgttc atccatcatc gtttctgagc
    28021 aagaaatcac ctcgcgactg aaagcaacag tcgcatcaca ggttgctgat tcagccggat
    28081 taaacagccg taaaatcact tcttgtcggt cttcagcttt cttaagtgcg cttatcaggc
    28141 atcccactgg gggcattttc aacaaactat aactttccgg cacgttgaat ccggctttgt
    28201 tgagcttcat cgcatcccat gggattttgt tgtaacactg tactggagtc agccatgctc
    28261 gcgcctgctg agctacacca gcggcggttg gcgtaccggt ataactcaat aaacttaggc
    28321 gacaagaaag cagaccacgt agttgtgagt ccgggactgg cattttaatt cccgaaggcc
    28381 gcctaggcct taaaagcaga tcttctttcc ccagtaagcc cacgccgcgc agcaacgtaa
    28441 tggcaaaggt tttcttctct tcaccgatga cttcaaattc acgtagccct tcgctaaaga
    28501 cagccatgcc gttacgccct tcctgtaagg ctgcatagtt gagcatattc cataccggaa
    28561 ccggcgcttc tttccagctt tcttgctgcc agttgttcat tgcactgtcg ttcacggggc
    28621 gcgttagcga accaaactgg gtatctgcca gaacactatc ggtgttaaaa gatgtaggga
    28681 tcaggacgcg aaggcgatga tcgtcagcct ggttatcaag gttgatatcc acatcaatgc
    28741 gcctgttgtt atgactaaga gtgacaacca actcgacatc tactctgcca gtggattgtc
    28801 ttgcgctgcg ttgttgcaga ttacgcggca ccgccatttc atagcggata acagccctgc
    28861 tctgccaggc ctcatgaata atctcgcatt gcggtttcgc attcgccgaa gtaattaccc
    28921 actcttcttt tgcgggtgaa tagtcatatt catcaccatc atcagagctt tcttcaattt
    28981 gcaacacccg gtcatagcgc acaccgctat ctttatctac cagttgtagc gttccatcct
    29041 cattgagcgc aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg
    29101 caatcacgtt gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga
    29161 caatctggtt gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta
    29221 tttgccgatc gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacggtacag
    29281 gctgaccgcg atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttga
    29341 tcacttcttc ccgcggccag ggcatcaggt taaacaggac gagtttgtcg gcgtcgctct
    29401 gcggcatgtt gtcggcgatt ttacgcatat agaaacgcag cagattatcc gccatgtctt
    29461 cagccagttc gaagcgggcg acgatttcgc gatgaacttt gtcacgacag cagcagccga
    29521 tgctgtcgtg ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt
    29581 ggtatccaaa acccaacgtc ctggccagcg ttgccagcgg ttccagcaga ttaacaatct
    29641 tattttcaat acgtgcgtgg gcaactttga tatccatgcg cgtggaaccg atggtgcgat
    29701 gcacgcgcat atatttgcca tcaataaatt cccctttcag ggttgccaga ttatctcgct
    29761 gcgcttcgat cttctcaaat acctcttcaa agcggctcat cacaaacttt cgttgaggat
    29821 aaatttcgcg cagtttctcc atcacttcga agatattttg ctgcaatggc atctgatcat
    29881 gcccattcgg cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac
    29941 tgtcgaggcg tttgcgtaat ccgttttcgt ccgcaggtaa gtacttaccg atggcataac
    30001 ccaacggcat cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg
    30061 ttttatccgt accgtggcgc tcagaacatc cacgccagaa catggtgcag gtaatgccaa
    30121 atccattgta gatatgcggc agttgcccgg acatgccaaa ggaatcgggt aaataaccta
    30181 ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga
    30241 tggattccgc agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact
    30301 tgccggcttc taccagtttc ttcaagcggt ctttgttttc cggtttcacc gcgaaataat
    30361 cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca
    30421 gtcggcacag gatctcttcc atattattga ccagcagaat gcgtgactct tcggtggtga
    30481 aataccactc ccgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca
    30541 tcgtcgtttc ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac
    30601 cgcgtgacgc cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc
    30661 accaaaccag ccaattgccg ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag
    30721 cgagaaaatg ccagcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc
    30781 cgttaccata gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat
    30841 cgggatcgcc ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc
    30901 aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc
    30961 cgaaaccatt ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc
    31021 gcccaggcag aatgcataag cggctttatt cactggccct ccaaggtcaa aggaacacat
    31081 aaaaccgaga atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc
    31141 ggtaagcgag ttattgatcc aggcgacagg ttcccccacc acaaacagca tcagactgcc
    31201 cgctcccaat gtaccgagca ccgggtagag ataaaaagtc aggaagccat tgaatttact
    31261 gctaagacgc aagtgatttt tcatccagcg tatcaagtaa ccggctatca atccgccaac
    31321 gaccgcgccg agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg
    31381 cgctaacgcc ggtttatctg ccagcgaata ggcggtatag gctgccagca ccggtaccat
    31441 caaaattccg agcagcccgc cgcccagttt gcggtacatc cacagccagg agttttcttc
    31501 attaaacaga tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt
    31561 cccccccgcg acaatcaacg gtacggcaaa agagatcccg ctcaacagtg cctgtttcag
    31621 ctccgttttg acgcttttca ccggttgcgt atcttgctgt aaggtacgcg tctcatcgcc
    31681 acgctcgagg gtaagcgatt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac
    31741 aggcacagaa agcgcaggaa tgccgttaaa acgctcactc tccttgatgg cgacttcagc
    31801 cgcaaaaata caggcagttg cactattgag ttgatccgcc gttaaacgcc cttcaatacc
    31861 gttagcacct tgcttttcaa cgtagacatt cacgccgagt ttgcgtccgg ctttttccag
    31921 atactccgca gccatgtagg tgtgagcaat acccgccggg caggccgtta cgcaaacgat
    31981 agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga
    32041 gagcaattca tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgccag
    32101 gcgcgtggtc agcgctgtca gcagttgcat gtgcgtagta cccgcttcat tgaggggaat
    32161 cgccagcaac accactaaat caaccgcttc cgggccatcc acgccttccc actgaagcgg
    32221 ctcgctgagt gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg
    32281 cacagccaac ccttcactta aggcggtcgg gccaaggctt tcacggcgat acacttcttt
    32341 cagaaattgc tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg
    32401 aatggcctct tcacggctgg taaagcgcgc attcagacac aacgcatcgc ggtgggtcag
    32461 agtcgtcagg ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg
    32521 acaaaacaat accattgcgt aatcaatttc acttgattga aattatttgc attttatttg
    32581 tattgtttac aggatgagtt tatcgggcat aatggctaaa ttcgtattaa tgagatgcta
    32641 aataatggga cacaaaccct tataccgaca gattgctgat cgcattcgtg agcaaattgc
    32701 gcgtggcgag cttaaacctg gcgatgcgtt acctaccgaa tcggcattac aaacagagtt
    32761 tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc aacagatcct
    32821 cgaaagcatt cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt
    32881 tcagttaacc agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaagt
    32941 tctgatattt gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgt
    33001 gcaggatcgt gtttggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct
    33061 ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa
    33121 ttcgaaatat cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga
    33181 aatcattcct ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa
    33241 gccgattctt gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag
    33301 ccgcaacgct tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc
    33361 gcgataagca caaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc
    33421 gccattcctg tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa
    33481 cagttttcag tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt
    33541 ccagagcagc gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt
    33601 ggcccatacg tttgcctttc ggcgcagtca caccagcgat gtaacccaca actggcttgg
    33661 taacatgctc tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga
    33721 tcatcacgat cgcttcggtc tgcggatctt tttcgaacat ttcgagaatg tcgataaagt
    33781 tagagccagg gatcgggtca ccgccgatac cgacacaggt cgactgaccg aaaccgtaat
    33841 ccgtggtctg tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt
    33901 tacccggttt gtgaatgtga cccggctgga taccaatttt gcattcaccc ggggtgataa
    33961 cgcctgggca gttcgggccg atcatacgaa cgcctgcttc gtccagtttc actttcacgg
    34021 tcagcatatc cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat
    34081 cgatagcttc cagaatggag tctttgcaga acggtgctgg tacgtaaata acagacgcgg
    34141 aagcgccagt agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg
    34201 tagtgccgcc tttacctgga gttacgccac caaccatttt agtgccgtat gcaatggcct
    34261 gttctgagtg gaaagtcccc tggctaccgg taaagccctg gcagataacc ttggtgtttt
    34321 tatcaattaa aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc
    34381 cgtcagacct tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag
    34441 ttcggcgttg ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttccgctac
    34501 cgcgccgatg ataccgtcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac
    34561 cagaacggct ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc
    34621 tttggttgcg ccgccgccaa cgtccaggaa gttagccggt tcgccgccgt gcagtttaac
    34681 tatgtccatc gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc
    34741 cagcgcaacg tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga
    34801 ctggtcacgc atttcgcgca gatcaggctg gcggaacagg gcgttgccat cagcgcccag
    34861 tttgccgtcg aggcaaatca gatcgccctg tttggtgatg accagcgggt tgatttcgat
    34921 cagcgccagg tcgcgctcca ggaaaatggt cgccaggccc atgaagattt tggtgaactg
    34981 ctgaaccagt ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat
    35041 cgggccagtc agcggatcaa gcgcaacttt atggatcagg tgcggagttt cttccgccac
    35101 tttttcgatt tccacgccgc cttcggtgga ggccataaag accacgcgac gggaactacg
    35161 gtctacaacg gcaccgagat acagctcttt agcgatatcg gtcgccgctt caaccagaat
    35221 ctggttaacc ggttggccat tggcatctgt ttgatacgtt accagacgct tgcccagcca
    35281 gttttctgca aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt
    35341 accgcggcca ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga
    35401 agcggcttct tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc
    35461 atagcgggca aacagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc
    35521 ttcagtaatc gttatctttt aaaccgtaga ccgacaccct atccacgtcg gtctacagtg
    35581 caggtgaagc ttaaactact acacgtccag cagcagacgc gtcggctctt ccagtaactc
    35641 tttgatcgtt accaggaagc ccacggattc gcgaccatcg atcagacggt gatcgtagga
    35701 cagcgccagg tacatcatcg gcaggatctc aacctgacca ttcaccgcca tcggacgatc
    35761 tttgatagcg tgcataccca gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat
    35821 cagggaaccg aacacaccac cgttggtgat ggtgaagtta ccaccggtca gatcttcaac
    35881 cgtcagcttg ccatcacggc ctttgactgc cagctctttg attttcttct cgatgtctgc
    35941 catgccgagg gtatcgacat cacgcagaac cggcgtcacc aggccgcgcg gcgtagaaac
    36001 cgccatgctg acgtcgaaat agttgtggta aaccacgtca tcgccgtcga tagacgcgtt
    36061 cacttccggg taacgtttca gggcttcaac caccgctttc acgtagaagg acataaagcc
    36121 cagacggatg ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca gatccataat
    36181 cggcttcatg ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt tcgcttccag
    36241 cagacgctct gccacacgct tacgcaggcg agtcatcggg acacgttttt cactacgtgc
    36301 agccagagcc ggttgcgccg ccggagcagc cgctgccgga gcagactctt tcgccggggc
    36361 tttcgccaga tgtttttcca catcttcacg agtcagacga ccacccacac cggtgccttt
    36421 aatggcgctg gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc
    36481 atcgttgttt tgctcttcca gagacgcctg ctggcgttgc gccggagtgg acgctttctc
    36541 ttcagatttg gcgctggttt ctttaccggc gctgttgcct tcacgcaggc gaccaaggat
    36601 ctgacgagac gttaccgttg taccttcatc ttccagaacc gcatccagaa tgccgtctgc
    36661 tgatgccggt acttccagta ccactttgtc agtttcgatt tctaccagca cttcatcacg
    36721 tacgactgcg tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg ctacggattc
    36781 aggcaggtca gggaccagaa tatctacgct actcattgtg tatcctttat ttattcgacg
    36841 ttcagcgcgt cattaaccag atcttgttgc tgtttctggt gaacggacat ataccctacc
    36901 gccggagagg cggaggccgg gcggcctgca taacgcagag aagccccaaa cggaatcact
    36961 tcacggaaat gatgctggct gcagtaccat gcgccctggt tgagcggctc ttcctggcac
    37021 cagacaaaat ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc tttatgcggg
    37081 aacgggtaga gttgctcgat acgcacaatg gcgacatcgt gttgattgtt cttacgacgc
    37141 tgttccagca ggtcgtaata aaccttacca gaacacatca ctacgcgctt cacgcccttc
    37201 ggatcaagct cgtcgatttc accgatggct ggcaggaagg tgccgttcgc cagttcttcg
    37261 aggctggaaa ccgccagcgg atgacgcagc agggatttcg gcgacatcac gaccagcgga
    37321 cgacgcatcc cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc cggggtagac
    37381 ggtacgcaaa cctgcatgtt ttgctcagca caaagttgca gataacgttc cagacgcgcg
    37441 gaggagtgct ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac cagaccacac
    37501 atccggcccc atttctgctc gccagaggag atgaactggt cgataaccac ctgcgcaccg
    37561 ttggcgaagt caccgaactg cgcttcccag atggtcaggg tgcgtggttc tgcggtggca
    37621 taaccatatt caaacgccag cacggcttct tctgacagta cggagtccca gacacggaac
    37681 gcgccctgac cgttatggat gtgttgcagc ggcgtgtaag tggaaccgtt agactggttg
    37741 tggatcaccg cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga
    37801 accggaatgc cttcatcaac cagcgtggcg taagcgaggt tttccgcacc gccccagtcg
    37861 aacagtttct caccggctgc catcgcctgg cgatcgccat aaatcttggc aacgcgagac
    37921 tgcatttcaa ctgcttctgg taccgtactg atacgtttag ccagttcctg caggcgcttc
    37981 atctcaactt tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg
    38041 aaagagtgca tgttcatcgg acgccactct gccactacgc aatcgccagc atccagcgca
    38101 tcgcggtaca ggttaaccat ctcggtggca tcttccagcg tcgccacttt ttcctgctcc
    38161 agcttgtcag cgtagatttt gcgcggtgtc ggatgttttt tgattttctg atacatcagt
    38221 ggctgggttg cgctcggctc gtcggcttcg ttgtggccgt gacggcggta gcataccagg
    38281 tcgatgaaga catcacgttt aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag
    38341 gcaacggctt ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg
    38401 atatcagtac agtacggtgt agaacgggca tccagcgggt tagaggtggt gaaaccaacc
    38461 tggttgttga taacgatacg taccgtaccg ccaacttcat aaccacgcgc tttcgacatg
    38521 ttcagagttt cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt
    38581 ggcagcactt tgttgctgtt cggctcatca agtctgtcca gacgggcacg aacagagccg
    38641 ataacgaccg ggcttacaat ctcaaggtga gacgggttaa acgccagcgc caggtgcacc
    38701 aggccgccat cggtctggaa gtcagacgag aagcccatgt ggtatttcac gtcgcctgtg
    38761 ccgaggtgtt ctttatgttt accggcgaac tcgtcgaaca agtcttgcgg ttttttaccc
    38821 agcacgttca ccagcacgtt cagacgacca cggtgcgcca tcccgagaac cacttcgcgg
    38881 gtgccgctgt tgccagcgtg gcggatcatc tctttaagca tcgggattaa cgcgtcaccg
    38941 ccttccagcg agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg ctcaaggcct
    39001 tcagcggcgg tcagttcgct taagaagcgt tttttctctt cgctattgaa agtcgcgcga
    39061 ccagactcga tacgctgttg gatccagcgt ttttcttcgg tgctggtgat gtgcatatac
    39121 tcggcaccaa tcgggccgca gtaggtttgc ttgagggcgg aaataagctc gccgagtttc
    39181 atggtttctt tgccgctggc aaatgaaccg acgttgaagg tttcctggaa gtctgcttcg
    39241 gtcagatcgt ggaaagacgg atccagatcg gccactttat cttgctgcca cagtcccagc
    39301 ggatctagat tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag ctgcaggact
    39361 ttaacctgct tcacattggt gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct
    39421 ttcgccaggc ggcggaaata ttcacgcgtt tgagagtgga attgatccgg tttgactccc
    39481 gtaccaggta actgctggaa cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt
    39541 aagaagtctt catagagctg ttctatccag ctctggtttg cgccagagag gtaagaagag
    39601 tccaaccagg ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc ttttttatgc
    39661 ttacttcgcc gtggatacta ccacgcacag tgatgtgcgt gccggggttc acttgcgagc
    39721 tcttgtctac gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag
    39781 tttttaaaga tttcctgcat ttctttgtta cgcctgatgc gctgcgctta tcaggcctgc
    39841 gggtaatgac ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat
    39901 gcaacgcttg cgcgtcttat caggcctaca gtttacgcat tacgttgcaa caacatcgac
    39961 ttgatatggc cgatggcgcg cgtcgggttc agccccttcg gacatacact gacgcagttc
    40021 atgatgctgt gacagcggaa tacgctgaat gcatcgctca aaccgtcgag gcggctgtca
    40081 gtctcggtat cacggctatc gatcaggaaa cgatatgccg ctaacaagcc tgccgggccg
    40141 ataaacttat cgggattcca ccagaaagac ggacaagagg ttgaacaaca tgcgcagaga
    40201 atacattcat acagcccgtc gagtttttcg cgctgctctg gcatctgtaa atgctcgcga
    40261 gctggcggat tttgtccatt attcaacagg taaggcttaa ttttctcata ttgcgcatag
    40321 aattgtccca tgtctaccac caaatcgcgg atcaccggta aacctggcag cgggcgaatc
    40381 acaatcttct tgcccggctg gttgagtgcc gaaatcgggg taatacaggc cagaccattc
    40441 ttgccgttca tgttcagacc gtcggaaccg cacacacctt cacggcagga gcggcggaac
    40501 gacaggctgg gatctttctc tttcagctgg ataagcgcat ccagcagcat catgtcgcga
    40561 ccttcttccg cttccagggt gtaatcctgc atacgcggag catcatcaac atccgggtta
    40621 tagcgataaa ttgaaaactc gagtctcatt ttcctgtctc cgcattagta agtacgaatc
    40681 ttcggcggga atgccgggcg cagtttcggt tccatgttga cgcttcggcg cgtcatggat
    40741 tccgactcag gcagatacag ggagtggcac agccagtttt catcatcacg atccgggaag
    40801 tcgaagcggc tatgtgcgcc acggctttcg gtacggaagt tggcagaaac agccgttgca
    40861 tacgccgttt ccatcaggtt atccagttcc aggcactcaa cgcgctgggt gttgaactcg
    40921 ctggaagtgt catccagacg ggcatttttc agacgctcgc ggatcacttt caactgctca
    40981 agccctttcg ccatcgcatc accttcacgg aagaccgaga agttatgctg catacattct
    41041 tgcagcgctt tacggatcgc caccggatct tcaccgttac gattattgtt ccaccggttc
    41101 aggcgatcca gagacgcttc aacatcagac tcgctggcat cgtgcagtgc gccctgctcg
    41161 gcgatagact cttgcagatg cagacctgcc gcgcgaccaa agaccaccag gtccagcagc
    41221 gagttgccgc ccagacggtt agcgccgtgt accgatacac aagcgatttc accaacggca
    41281 aacagtcccg gaacaaccac atcttcgcct ttctcattca cagtcagcgc ctgaccggta
    41341 actttggtcg gaataccgcc catcatgtag tgacaggttg ggataaccgg aatcggctct
    41401 ttcaccggat cgacgtgagc gaaggtacgg gaaagctcca ggatacccgg cagacgggat
    41461 tcgagaactt ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc ccacggacca
    41521 tcacagccgc gaccttcacg gatttcaatc atgatggaac gcgccaccac atcacggccc
    41581 gccaggtctt tggcgttcgg cgcataacgc tccataaagc gttcgccatg tttgttcagc
    41641 agataaccgc cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc ggcaatgccg
    41701 gtcgggtgga attgccacat ttccatatcc tgcaccggta cgcctgcgcg gatagccatg
    41761 ccgacaccgt cgccagtgtt aatgtgggcg ttagtggtgg actgataaat acgccctgct
    41821 ccgccagtcg ccagcacggt agcgcgggct ttgaaataaa ccacttcacc ggtttcgatg
    41881 cacagtgcgg tacaacccac caccgcgcca tcctggtttt tcaccagatc cagcgcatac
    41941 cactcggaga aaatggtggt gtggtttttc aggttctgct gataaagcgt gtgcaacagt
    42001 gcgtgaccgg tacggtcagc agccgccgca gtgcgtgccg cctgctcgcc gccgaagttt
    42061 ttcgactgac cgccaaacgg acgttgatag atacgaccat catcgagacg cgagaacggc
    42121 aggcccatgt gttcgagttc cagaatcgct tccggcccgg ttttacacat atattcaatc
    42181 gcgtcctggt caccgatata gtccgaccct tttacggtgt cgtacatatg ccattcccag
    42241 ttatcttcat gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga aacggtatgg
    42301 gaacgggtcg ggaagacttt agagagcagg gcacaggtct ggccgctctg ggaaatttgc
    42361 agcgccgcgc gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa ttctctgact
    42421 ggcaatttca tcacacaccc cacaccacaa cgaatccata aatcacgtaa accaccagtg
    42481 caacgacaat caccagttgc agcatcaggc gcaaggccag cggtttaacg tagtcggtca
    42541 acacctgcca catgccgatc caggcatgaa tcaagataga aaacagcgcc agcagggtga
    42601 acactttggt gaacgcagag gcgaagaaac cgatccagac ttcatatgtc agttcgccac
    42661 tggtggcgaa aaaaccgacc atataaatga tgtagagcgt caggacgata gcggtagcgc
    42721 gaacgaggat gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg cttaccatac
    42781 gaggactcct gcgagaagtg aaagcacgac agtaataaca aaggagattt tggcggagcg
    42841 tttacccgct tcgaatgttt cttccagata gccaaaatcc atcatcatgt ggcgaatacc
    42901 tacgacgacg tgatacgcca gagcggtaag gatgccccac atgataaatt tgacgaagaa
    42961 gctgcccata atcgcggaag cttgctcgaa accttcaggg gaagagaggc tggtacccag
    43021 aagccactgc aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc gatggagaat
    43081 ggacgctatc gccgtgatgg ggaaccggat ggtctgtagg tccagattaa caggtctttg
    43141 ttttttcaca tttcttatca tgaataacgc ccacatgctg ttcttattat tccctgggga
    43201 ctacgggcac agaggttaac tttctgttac ctggagacgt cgggatttcc ttcctccggt
    43261 ctgcttgcgg gtcagacagc gtcctttcca taactgcgcg tcatgcaaaa cactgcttcc
    43321 agatgcgaaa acgacacgtt acaacgctgg gtggctcggg attgcagggt gttccggaga
    43381 cctggcggca gtataggctg ttcacaaaat cattacaatt aacctacata tagtttgtcg
    43441 ggttttatcc tgaacagtga tccaggtcac gataacaaca tttatttaat ttttaatcat
    43501 ctaatttgac aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc
    43561 gtaaaagtta tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat
    43621 tgaccaattc attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa
    43681 gtacttgatt cgccatttat tcgtcatcaa tggattcttt acctgcaagc gcccagagct
    43741 ctgtacccag gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc
    43801 caggttgatg tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg
    43861 agaccttaaa tggctgatac aaaagcaaaa ctcaacctca acggggacac agctgttgaa
    43921 ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca
    43981 aaaggtgtgt tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt
    44041 acttttattg atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg
    44101 gcgaccgatt ctaactacct agaagtttgt tacatcctgc tgaatggtga aaaaccgact
    44161 caggaacagt atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag
    44221 attacccgtc tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt
    44281 attaccggcg cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcat
    44341 cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac
    44401 aagtattcca ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac
    44461 ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa
    44521 cgtgctatgg accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc
    44581 accgtgcgta ccgctggctc ttcgggggcg aacccgtttg cctgtatcgc agcaggtatt
    44641 gcttcactgt ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa
    44701 gaaattagct ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat
    44761 tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc
    44821 gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg
    44881 gaagtggcta tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa
    44941 ctgtacccga acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct
    45001 tccatgttca ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc
    45061 gaaatgcaca gttacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa
    45121 aaacgcgact ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt
    45181 taacccgccg ttcaaatggc gggttaattt ttatatgcct aaacacaaaa aattgtaaaa
    45241 ataaaatcca ctatcggaca ttcatatatt ctaaaaaaaa atacatcggt ataaattata
    45301 aacaaataga tgatttttaa tcatctatct tcctctcagt tacatgaata aaccaccaaa
    45361 tatagcgcac ccctataatt cccaatactt tattcatcaa acatttaatt cataccatca
    45421 attaattact tttacattta ataaaattaa taatattttt aagaaaaact aaaaacattc
    45481 gacaatcaaa taagatattc ttgcaccgat atactttttt cttttctgtt aaaggactca
    45541 gttatgttta aaggacaaaa aacattggcc gcactggccg tatctctgct gttcacagca
    45601 cctgtttatg cggctgatga aggatccggt gaaattcact ttaaaggtga agttattgaa
    45661 gcaccgtgtg aaatacatca ggatgatatt gataaagagg ttgaactcgg tcaggtgacc
    45721 accagccaca ttaatcagtc acatcacagc gatgccgttg ctgtcgacct gctcttagtc
    45781 aactgtgatc tggaaaactc cagcaacggt tccggtggca agatttctaa agttgcagtc
    45841 acctttgata gctcagcgaa aaccaccggc gcagatccga ttctcaacaa caccagcacg
    45901 ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag atcaaagcaa tatcgtttta
    45961 ggcaccgcta ctccagatat cgacctggct ccgacctcca gcgaacaaac gctgaatttc
    46021 tttgcctgga tggaacaaat tgatcaggct acacctgtaa cgccaggcgc agttacggcg
    46081 aatgcaacct acgtgctgga ttataaataa gtattacttt ggcggcgcta tcgccgcctt
    46141 tcataaaata tttatcaggt atggacaccg tgaatattta tcgactctct tttgtatcct
    46201 gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt caatctgaat gttctcgata
    46261 aatcaatgcg cgaccgcatt gatatttcat tattaaagga aaaaggagtc attgctcccg
    46321 gtgagtattt tgttagcgtt gcggtaaata ataaccaaat cagtaacgag caaaagatta
    46381 actggcacaa aaatgacgat aaaaccattc cgtgcatcaa tgatttactg gtcgataaat
    46441 ttggcttaaa acctgaagtc cgtcagtcgt taccattgat aaatcagtgc gtcgatttta
    46501 gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa tcagcaactg aatattagta
    46561 ttccgcaagc ctggctggcg tggcactcag aaaactggac cccaccctcc acatggaaag
    46621 aaggtgtcgc cggtgtcctg atggattaca acttgtttgc cagcagctac cgcccacagg
    46681 atggcagcag cagcactaat cttaacgcct atggaacagc cggaatcaac acgggggcat
    46741 ggcgcttacg cagtgattat cagttgaatc atactgatag cgatgataac catgaacagt
    46801 caggcgaaat atcacgtacc tatctttttc gcccgttacc gcaattaggt tcaaaattaa
    46861 ccctcggcga aacggatttt agtcccaata ttttcgacgg tttttcttat accggcgcgg
    46921 cactggcaag tgacgatcga atgttgccat gggaactacg cggctacgcc ccacaaatta
    46981 gcggtattgc acagaccaat gccacggtga cgatcagtca atcaggccgc gtcatttacc
    47041 agaaaaaagt cccaccaggc ccatttatca ttgacgacct taatcagtct gttcagggca
    47101 cactggatgt caaagtgacg gaagaagatg gtcgggtgaa caatttccag gtttcagcag
    47161 catcgacgcc cttcctgact cgtcagggac aggttcgcta taaactggcc gcgggtcagc
    47221 cacggccctc catgtcacat caaactgaaa atgaaacctt ttttagcaat gaagtttcct
    47281 gggggatgct gtcaaacacc tcgctgtaca gcggcctgct gctttctggt gatgactacc
    47341 attctgccgc aatgggtatt gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg
    47401 tcacgtgggc cagtagccat tttgatactc agcaggacga gcggggctta agctaccgtt
    47461 ttaattacag caaacaagtg gatgccacta acagcacgat ttccctcgcc gcttatcgtt
    47521 tctccgatcg tcattttcac agctacgcca actatctgga tcacaaatac aacgacagcg
    47581 atgcgcagga cgaaaaacag acgatcagct tatctgtggg ccaaccgatt accccactaa
    47641 acctcaatct ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga
    47701 ccgccaacat cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt
    47761 ctacgtcatt caatacaacc cattacgaag ataaagatcg cgacaaccag atttacctgt
    47821 cgatttcgct ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc
    47881 acagcaccac acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca
    47941 tgtctgccgg actgcaatcc gaccgtcctg acaatggagc ccaggtgagc ggtaactatc
    48001 agcacctgag ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca
    48061 gttccgtcag cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc
    48121 atcgccgcag ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata
    48181 ttccggttca gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga
    48241 tttccagtta ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa
    48301 cagttgcaga aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac
    48361 tggcttcccg ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc
    48421 ctcccctcgg agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg
    48481 aggaaggaca tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg
    48541 gtgatagcca gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc
    48601 tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct
    48661 tttgttgttg gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat
    48721 tgtgtttaac gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact
    48781 cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct
    48841 ggtcgccctg ccgccgatac aacgtctgga accgaaagca acaacacagg tacggatagt
    48901 aaagcaagcc tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat
    48961 gcgtgaaatt cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat
    49021 tcagagtcgt atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa
    49081 ggtcgaactg cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac
    49141 cgcgtattat ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt
    49201 caaaaccgtg atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg
    49261 cagccagttt tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa
    49321 ctgcagcgga caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga
    49381 gtgttgctcg tcatctgtct gttatttctg tcgttgaagt ctgcgcttgc cctgaactgc
    49441 tactttggtt catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta
    49501 ccaggcaatg ccaaactcgg cgataagatc tgggaatctg acgatattaa aataagatct
    49561 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg
    49621 aaagcgaaca tgtctacgta tgggtaaacc cctacccggg ggtgcaggat cgttattatc
    49681 aactcggagt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
    49741 acactaacca gtgtatcgac agtaagaaca ttgacattta tacccctgat gggatggcag
    49801 aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgaacctt tctcgctcgc
    49861 atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc
    49921 gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa
    49981 aacctgaaat atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat
    50041 ttttccattt caccggaaac acagatgatt gattttggta aatttaattt gctggatatt
    50101 cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa
    50161 tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa
    50221 gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg
    50281 ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac
    50341 gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc
    50401 ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca
    50461 gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt
    50521 attttttcct agcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag
    50581 ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc
    50641 tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg
    50701 gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc
    50761 gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct
    50821 ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat
    50881 cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc
    50941 ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg
    51001 cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt
    51061 cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc
    51121 gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg
    51181 atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct
    51241 ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat
    51301 ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg
    51361 cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg caggtatggc
    51421 gtggggactg acccggttta tgcatattga ttttatgact gcctacctcg ccaccagtcc
    51481 cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat
    51541 tatggcgatg caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg
    51601 gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta
    51661 aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc
    51721 gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac
    51781 ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc
    51841 cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag
    51901 gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc
    51961 tgcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca cctccggcgt
    52021 cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg
    52081 ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt
    52141 ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg
    52201 ccagacgccg taaagctgat tatggctgta gagcgttaaa ccgttgggaa aatgagttaa
    52261 caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata
    52321 agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc
    52381 ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt
    52441 tcattcttta ttaagcgggg ttatgctgcg acaacaatcc cctttttggc aaatgtagct
    52501 cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca gaccgtttgc
    52561 gccgcgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctcc
    52621 agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc
    52681 accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg
    52741 ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgcc
    52801 ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc
    52861 tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca
    52921 atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc cggcggcagc
    52981 tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg
    53041 acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga
    53101 aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag
    53161 ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg ctaattgttc
    53221 aaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa
    53281 atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca
    53341 ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc
    53401 gttatgcggc acctgcacca cgcccggtaa caaaccgtga gataacagtt cgcgatcggt
    53461 gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt cggttacttt gcgagctaag
    53521 ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg
    53581 ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat
    53641 aaaatcacgc ttcgatttgc caatcgggag tcagtcacca tccttcagta aacggccttc
    53701 cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg gcggaacatc
    53761 aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa
    53821 acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcgttat catccaaccg
    53881 cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca gtttcgaact caactgttaa
    53941 ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc
    54001 aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg atacccgact ggcaaaaacc
    54061 gtgacgaccg ccatcctgca cagtggtgta catgcccgca cgaataatct tcagcatatt
    54121 ccctccttct gcggtacaaa tcgcacgctg tctcccggac gtaataagat gggttcgtca
    54181 cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt
    54241 gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc
    54301 aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg
    54361 aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca
    54421 acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg tccgcctgca
    54481 ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc
    54541 tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa
    54601 atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga
    54661 cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc
    54721 agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct
    54781 gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc
    54841 attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt gacctgcagc
    54901 ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt
    54961 aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt
    55021 gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg
    55081 tcccagacgc gcttcaatcc aggaggctag ttccattccc ggcacaggca tggtcagctc
    55141 tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag
    55201 ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt
    55261 gatatcattc gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga
    55321 ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc
    55381 ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt
    55441 gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc
    55501 gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct
    55561 acaccgttaa catagcgtta catcgcctgt tcgtcgatag ccaatctttt gacatattaa
    55621 gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta
    55681 tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact
    55741 ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt
    55801 attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc gctgcaaatc
    55861 tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa
    55921 ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat
    55981 gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg
    56041 atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat
    56101 ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg gctatggcct gtttaaatct
    56161 aacgtaagct gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt
    56221 ttctcgctga tgtatgcggc gggtaacgtg gggtctatta tcgcacctat cgcctgtggt
    56281 tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt tggaatgatc
    56341 gcgggtctgg tcattttctt atgtggcaat cgtcatttca ctcatacccg cggcgttaac
    56401 aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact ggggatggct gctggttctg
    56461 ctggtggcaa cgcctgcact gattaccgtg ctgttctgga aagagtggtc ggtatacgcc
    56521 ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa
    56581 aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg
    56641 ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga ccgcttcgtt
    56701 aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat taatgccttc
    56761 gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat
    56821 cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag cgccggattc
    56881 tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg
    56941 atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg
    57001 tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg
    57061 ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag
    57121 gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt
    57181 gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat
    57241 caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccgat
    57301 gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa tttattgaat
    57361 ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca tccggcatct gggctcgatt
    57421 gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa cgtttgtact
    57481 ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc tgctttctgc
    57541 gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat
    57601 cgacgaataa actcgccctc acggtcaaat ttctcgccct gagttatcgg attgaaaata
    57661 cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc accgttatta
    57721 gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg
    57781 atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag
    57841 ccagtgctgt taagctgacg catagcggca tcaataatcg ggtatcccgt tttgcctttc
    57901 tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt ccaggcaata
    57961 aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc
    58021 cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc
    58081 gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa gctggcggac
    58141 aaacggctgg tgccttccac tgccggaaaa tctcgttgtt gctcatactc tccagcaccg
    58201 ttctcgcaaa actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca
    58261 gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt tatcgatccg
    58321 ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag ccgtttcagc
    58381 caggcattct taaaaggcgt aaagactttg tacatctcgt gattgccggt catcaccgcc
    58441 cccggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt
    58501 tcaacgtcca catcccgcgc tcgctcattc acctcatact gatagttata aaaaagatga
    58561 gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc
    58621 acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag cccattcagt
    58681 tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtctccca cttgcgtggt
    58741 gtagcgatat acagcgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg
    58801 agattatcgt gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc
    58861 ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc
    58921 aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt
    58981 gctcgccacg gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa
    59041 tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg
    59101 cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat
    59161 tttgccactg atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat
    59221 gggccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc
    59281 catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat
    59341 caaatcgttg tagattcatt actgctcctg aacggtatac aggagtaagt gtaggcgttg
    59401 gtaaatgatg tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc
    59461 gcaagcaact aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcact
    59521 atttccaaaa cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc
    59581 aaaaccggta tagcccggta ttattcacaa catcccagtc tgcaccttaa gggcgactgg
    59641 ctgaaagagg ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggggc
    59701 ctgaccatca tcgctgacag tgacgaggtg caggaactgc gggatgggat gtttagtgtg
    59761 ctgaacgaaa gttaaatatt aaaaaagctg ggaagattta aaaccttcct agctttttat
    59821 tctgtcttat tataatggat actcatcatc ttccgatgaa tttataaatt gctccaagtt
    59881 tacatgaaga gtacttaacc tagtttttaa ctcattaagt gaagcgataa tttcatattt
    59941 tccaacataa tcatatgatg gatcgtattc attatctatg tcagaagcta aaaatgatga
    60001 tattcgtagt ttaaatttag cttcatcttt attacattca ttggcataag gtgaatgccc
    60061 tatcatagca tattcatcta atctaaaagg ataagatggt tttaattcat cgatcaatga
    60121 aatttcaatc catgttctat atccaggcat taatggacta atttctttga gcaaaacctt
    60181 tccacttaat aatgtattga gattgttcat tattatgtct cccacgggaa aaatatatgt
    60241 ctaacacttc caccatgttg atgaacttgt aaatgtggta aatcaccatc aggatttcct
    60301 ggcccatgac ctgacactct atatgaaggt tttgttacag aaggatcttt tatatcatcc
    60361 caatgatatg aacctgcttt tgttttattt tcagtaagat attccattaa gttcttagtg
    60421 ctggggcctg actcacctgt catattttta taattatgac ctaaatatct atccataaac
    60481 aattgttcag cgtcacttct agacctaaca gtaactttgg tttcactact atctaataaa
    60541 cgattcgctt tttggttact aacagcacat ggagttaatc caagtggatc agcatatttt
    60601 agaggattag gtgcataact gtagagattt aaaccgcctc tcaaccctat cgggtcagtt
    60661 accatgaacc gcccgacatc cggatcatag tacctgtgca gattgtagtg cagccccgtt
    60721 tccctgtcga ggtactgtcc ctggtagcgc aggttctgct ccaccccgcc gtggtccttc
    60781 tcctgtatcg tgttacccca gacctgatac cccgtctccc acacgatttt cccgtcgctg
    60841 tccgtcatct cttccggcgc gccgttcaca tccgtgtgaa aatacagtat ccggttcggg
    60901 ccctctttgc ccgccttatc cacccgcgcc agcggttcat aactcccctg gtccctgtag
    60961 atgtacaggc tgcctgttcc ctccggcctg ctctcctgcg ccagccgcat tccgtcccat
    61021 aaaaattttg tccggttgta cggcttaccg tcagggctga tgtgctgttt ttccgtccgc
    61081 ctccccagtg catcgtacac ataccggtat gtcccccgcg ctgtcgtcac ttccaccatc
    61141 cggtgttccg catcataacg gtaactctgc gttccgctcc ggccctcctt ttccaccgca
    61201 cggccaaatt cgtcataacg gtagtgcaca ccacggtaag acatcagccg gttgaacggc
    61261 accacgctgt tttctgccgt ggccctttcc cccgcgcgcc tccccagcag gtttcccgcc
    61321 gcgtccacct ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc
    61381 cccagcgggt cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg
    61441 ccgccctgag ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc
    61501 gtcacctgtc cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga
    61561 taacgtgtgc gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc
    61621 agccggtact gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc
    61681 ggaccttcat accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc
    61741 gtcatgcagt ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac
    61801 acgccgttca ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc
    61861 tcagggaaat gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac
    61921 agacggatgc acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg
    61981 tagtacagat tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc
    62041 gggtggtgcg ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag
    62101 tgcccctgct caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc
    62161 ggcagtacgg cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt
    62221 tcgtctgcgc tacccggtgt gcacacgagg ctgacttcat acattacact ccagccctga
    62281 ccaaacattc cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga
    62341 aagtggcccg gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg
    62401 gcggcattga ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg
    62461 acactggcag caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc
    62521 gccacggttg ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt
    62581 aacggaccgc caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc
    62641 accctggcct cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc
    62701 acatcccgcg cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac
    62761 agattactga tgctgccaat aaccgcattc actccctgaa aggcggtttc cagcataccg
    62821 cccggattca gcaggttaag ggccgttccc aggggggcat ccagaaaggc ggcttttttt
    62881 tgtgcgagaa actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg
    62941 gtcggggtgg atcccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa
    63001 tgtgtcacgc catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga
    63061 gcccgggaca tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc
    63121 atttccagat attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct
    63181 tcttcctgtt ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca
    63241 gtcgccgctg cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg
    63301 atgtaacctt caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg
    63361 accttttctg tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg
    63421 acggccgtgc accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca
    63481 atgagggcat caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc
    63541 ataaacgacg tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg
    63601 tcccgttccc tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc
    63661 tttttaattt cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag
    63721 gtgaaggcca tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc
    63781 tgctcgttac cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt
    63841 ccgtcataat gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt
    63901 tttaatcatg aatgtaatac ccatcaccgc tttacagcac actttatctt tccagtcata
    63961 atcactaata atattaagca attagcaata tttacaacga ccagagcgga tacatagtta
    64021 tgtatttaac aatccca
//
LOCUS       DAJTRM010000011        60585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRM010000011 DAJTRM010000000
VERSION     DAJTRM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.011"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            545..1834
                     /gene="sdaC"
                     /locus_tag="OO322_000762"
     CDS             545..1834
                     /gene="sdaC"
                     /locus_tag="OO322_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5905748.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="OO322_000763"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="OO322_000763"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5905749.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3370..4125
                     /gene="xni"
                     /locus_tag="OO322_000764"
     CDS             3370..4125
                     /gene="xni"
                     /locus_tag="OO322_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5905750.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO322_000765"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="OO322_000765"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5905751.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO322_000766"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="OO322_000766"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5905752.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="OO322_000767"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="OO322_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5905753.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="OO322_000768"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="OO322_000768"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5905754.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="OO322_000769"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="OO322_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="OO322_000770"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="OO322_000770"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5905755.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="OO322_000771"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="OO322_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5905756.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO322_000772"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="OO322_000772"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5905757.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="OO322_000773"
     CDS             complement(13550..13945)
                     /locus_tag="OO322_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5905758.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO322_000774"
     CDS             complement(13964..14881)
                     /gene="gcvA"
                     /locus_tag="OO322_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5905759.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="OO322_000775"
     CDS             complement(15232..15459)
                     /locus_tag="OO322_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5905760.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="OO322_000776"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="OO322_000776"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5905761.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="OO322_000777"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="OO322_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5905762.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="OO322_000778"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="OO322_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5905763.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18394..19491)
                     /gene="mltA"
                     /locus_tag="OO322_000779"
     CDS             complement(18394..19491)
                     /gene="mltA"
                     /locus_tag="OO322_000779"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5905764.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19700..19776
                     /locus_tag="OO322_000780"
     tRNA            19700..19776
                     /locus_tag="OO322_000780"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Met,seq:cat)
     gene            19810..19886
                     /locus_tag="OO322_000781"
     tRNA            19810..19886
                     /locus_tag="OO322_000781"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19844..19846,aa:Met,seq:cat)
     gene            complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="OO322_000782"
     CDS             complement(19961..21214)
                     /gene="amiC"
                     /locus_tag="OO322_000782"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5905765.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21446..22777
                     /gene="argA"
                     /locus_tag="OO322_000783"
     CDS             21446..22777
                     /gene="argA"
                     /locus_tag="OO322_000783"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5905766.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22839..24665)
                     /gene="recD"
                     /locus_tag="OO322_000784"
     CDS             complement(22839..24665)
                     /gene="recD"
                     /locus_tag="OO322_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5905767.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24665..28207)
                     /gene="recB"
                     /locus_tag="OO322_000785"
     CDS             complement(24665..28207)
                     /gene="recB"
                     /locus_tag="OO322_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5905768.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="OO322_000786"
     CDS             complement(28200..31088)
                     /gene="ptrA"
                     /locus_tag="OO322_000786"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5905769.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31264..34632)
                     /gene="recC"
                     /locus_tag="OO322_000787"
     CDS             complement(31264..34632)
                     /gene="recC"
                     /locus_tag="OO322_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5905770.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34645..34995)
                     /locus_tag="OO322_000788"
     CDS             complement(34645..34995)
                     /locus_tag="OO322_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5905771.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34980..35387)
                     /locus_tag="OO322_000789"
     CDS             complement(34980..35387)
                     /locus_tag="OO322_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5905772.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35384..35947)
                     /locus_tag="OO322_000790"
     CDS             complement(35384..35947)
                     /locus_tag="OO322_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5905773.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35938..36408)
                     /locus_tag="OO322_000791"
                     /pseudo
     CDS             complement(35938..36408)
                     /locus_tag="OO322_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36592..37386)
                     /gene="thyA"
                     /locus_tag="OO322_000792"
     CDS             complement(36592..37386)
                     /gene="thyA"
                     /locus_tag="OO322_000792"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5905774.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37393..38268)
                     /gene="lgt"
                     /locus_tag="OO322_000793"
     CDS             complement(37393..38268)
                     /gene="lgt"
                     /locus_tag="OO322_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5905775.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38419..40665)
                     /gene="ptsP"
                     /locus_tag="OO322_000794"
     CDS             complement(38419..40665)
                     /gene="ptsP"
                     /locus_tag="OO322_000794"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5905776.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40678..41208)
                     /gene="rppH"
                     /locus_tag="OO322_000795"
     CDS             complement(40678..41208)
                     /gene="rppH"
                     /locus_tag="OO322_000795"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5905777.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41893..42582
                     /gene="mutH"
                     /locus_tag="OO322_000796"
     CDS             41893..42582
                     /gene="mutH"
                     /locus_tag="OO322_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5905778.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42651..43364
                     /locus_tag="OO322_000797"
     CDS             42651..43364
                     /locus_tag="OO322_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5905779.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43502..43720
                     /gene="ygdR"
                     /locus_tag="OO322_000798"
     CDS             43502..43720
                     /gene="ygdR"
                     /locus_tag="OO322_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5905780.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43828..44868
                     /locus_tag="OO322_000799"
     CDS             43828..44868
                     /locus_tag="OO322_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5905781.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44901..46091)
                     /gene="lplT"
                     /locus_tag="OO322_000800"
     CDS             complement(44901..46091)
                     /gene="lplT"
                     /locus_tag="OO322_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5905782.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46084..48243)
                     /gene="aas"
                     /locus_tag="OO322_000801"
     CDS             complement(46084..48243)
                     /gene="aas"
                     /locus_tag="OO322_000801"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5905783.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48829..49860
                     /gene="galR"
                     /locus_tag="OO322_000802"
     CDS             48829..49860
                     /gene="galR"
                     /locus_tag="OO322_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5905784.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49867..51129)
                     /gene="lysA"
                     /locus_tag="OO322_000803"
     CDS             complement(49867..51129)
                     /gene="lysA"
                     /locus_tag="OO322_000803"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5905785.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51251..52186
                     /gene="lysR"
                     /locus_tag="OO322_000804"
     CDS             51251..52186
                     /gene="lysR"
                     /locus_tag="OO322_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5905786.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52173..52865)
                     /gene="ygeA"
                     /locus_tag="OO322_000805"
     CDS             complement(52173..52865)
                     /gene="ygeA"
                     /locus_tag="OO322_000805"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5905787.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52994..54412)
                     /gene="araE"
                     /locus_tag="OO322_000806"
     CDS             complement(52994..54412)
                     /gene="araE"
                     /locus_tag="OO322_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5905788.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54727..55488)
                     /gene="kduD"
                     /locus_tag="OO322_000807"
     CDS             complement(54727..55488)
                     /gene="kduD"
                     /locus_tag="OO322_000807"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5905789.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55518..56354)
                     /gene="kduI"
                     /locus_tag="OO322_000808"
     CDS             complement(55518..56354)
                     /gene="kduI"
                     /locus_tag="OO322_000808"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5905790.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56641..57822)
                     /locus_tag="OO322_000809"
     CDS             complement(56641..57822)
                     /locus_tag="OO322_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5905791.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58077..59306
                     /locus_tag="OO322_000810"
                     /pseudo
     CDS             58077..59306
                     /locus_tag="OO322_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            59941..60585
                     /locus_tag="OO322_000811"
                     /pseudo
     CDS             59941..60585
                     /locus_tag="OO322_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT        14969 a        16262 c        14770 g        14584 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc
      121 atacaattta tgttgcaaca caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atcgccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacacaggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcaacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcctgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattctaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtttcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatgcc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg ctgcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttagt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccag cgaattcact actgatatca cgatggccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacaac gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatggaacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gattaaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccga tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaaacgatt
    15481 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagagctgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg acggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcaa
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tgggattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aagttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcccctgt
    18361 catatccgta aaaacggcat acagaatatc acatcagccg ctaaagacgt tacctgcgcc
    18421 cggagccgtt ttcagcaccc aaacccgtcc atagtggttg taccaacctg cgcggtgtcc
    18481 ggcttccggc ccgatccctt gatagatatc gaagtgttgg cctttgattg cgcccccgac
    18541 atccaacgcc accatcagac gcaattcgta ctgaccgtta aatttgccgt tattatccag
    18601 cagcggcact tctgccagca aggtagtacc tggcggaata attgaacgat cggaggcaac
    18661 agaagcacgg ccaaccagcg gcaccgcact tgcccctttc accggtgcaa aagattgcgg
    18721 tttaaagaag acgaaagacg ggttctgttc cagcagctcg cgaacctcgg cttcactgtg
    18781 tgtttcgccc caatgacgaa tcgcctgcat cgacatatct tcttttttca cttcgccacg
    18841 gtcgatcagc accttaccaa tgctgcgata ggcatgaccg tttttccctg catagctgaa
    18901 aaagttaagc ggactgccat caccaaagtc gatataccca ctaccctgca catccataat
    18961 gaagttatcc atcagagagt tgctgtaagc gaggatgtat ttatcgccca atgccccggc
    19021 gtagatctcc gcacgagacg gcagacgacc acgttttggc ggcatacggt aaataggata
    19081 ctggaactcg ccctggcggg tatggcgcgc ctgaattacc ggcgtgtaat agcctgtaaa
    19141 ctgcacgtta ccatagttgt cggcaccttc catctgccag gcatcaatgc cgaactggcg
    19201 catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc accgcgttat aaacattact
    19261 ctggttgcca tacagacgcg gtgacgaatt acggatatgg ttaatttgct cggcaaaatc
    19321 accggcgtta atcggcgcgc caacggcatc tggctggttc accagagaga aaggctgggt
    19381 aaatttcccg tctttatatt gctgtccgcg atcggttggt ttggaagagc aggcggcaag
    19441 cattgccaca accgtgccca taagaaggta ctttacccaa cgtcctttca ttgttctctt
    19501 cttcaggtta aaaaataagg cgcaacgaag ataacaaacc gccgcgttta atgaaatgag
    19561 cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct ttagtggttt gtttttgttc
    19621 aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag
    19681 tatagtgcgc atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc
    19741 cgaaggtcgt cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca
    19801 gtacggtgac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19861 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    19921 ctgatctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    19981 ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
    20041 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    20101 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
    20161 ttttatgcag cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc
    20221 tgtcagcaat agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    20281 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
    20341 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac
    20401 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
    20461 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    20521 tattgccctc ttcctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt
    20581 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
    20641 gcataatgac aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg
    20701 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    20761 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    20821 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc
    20881 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    20941 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    21001 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    21061 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    21121 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    21181 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    21241 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    21301 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    21361 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    21421 aaataatcat gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
    21481 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    21541 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    21601 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    21661 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    21721 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    21781 gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa
    21841 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    21901 tatccggcgg attgatgaag acgcgctcca tcgtcaactg gaaagcggtg caatagtgct
    21961 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    22021 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    22081 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
    22141 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    22201 tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga
    22261 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    22321 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    22381 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    22441 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    22501 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    22561 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
    22621 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    22681 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    22741 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg
    22801 caggcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    22861 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    22921 tcggcataca gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    22981 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    23041 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    23101 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    23161 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
    23221 accggtcggc cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    23281 ataaactgtt caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    23341 cacaaaagct ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    23401 agcagatcca gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct
    23461 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    23521 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    23581 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    23641 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
    23701 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    23761 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc
    23821 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    23881 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
    23941 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    24001 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    24061 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
    24121 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    24181 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    24241 tccagggttt gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag
    24301 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    24361 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    24421 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg attcgacgcc
    24481 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    24541 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
    24601 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    24661 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    24721 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    24781 ggaacagata gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc
    24841 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    24901 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    24961 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    25021 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    25081 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    25141 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    25201 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    25261 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    25321 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    25381 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    25441 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    25501 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    25561 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    25621 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    25681 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    25741 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    25801 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    25861 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    25921 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    25981 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    26041 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    26101 tttccagctg cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac
    26161 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    26221 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    26281 cgactaccgc atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    26341 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    26401 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    26461 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    26521 cctggcggct gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    26581 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    26641 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    26701 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    26761 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgtg tcatcagtct
    26821 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    26881 aagtgtagtg ggcatgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    26941 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    27001 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    27061 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    27121 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    27181 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcaac agatcgcgga
    27241 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
    27301 cccccccggc cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    27361 gcaactgata actgtttgtc tcttcttctg cccaggcgct gatcttttcg atccatttag
    27421 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    27481 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    27541 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcggttcgc
    27601 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    27661 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    27721 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    27781 caaaggcatt caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt
    27841 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    27901 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    27961 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    28021 tgaccaccag cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    28081 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    28141 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    28201 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    28261 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    28321 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    28381 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    28441 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    28501 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    28561 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    28621 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    28681 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    28741 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    28801 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    28861 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    28921 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    28981 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    29041 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    29101 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    29161 tggttatacc aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac
    29221 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaatc
    29281 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    29341 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    29401 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg
    29461 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    29521 ggatggtcgt atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta
    29581 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    29641 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    29701 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    29761 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    29821 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    29881 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    29941 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    30001 gccaggcctt tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    30061 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa
    30121 agtgtacctg ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg
    30181 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca
    30241 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    30301 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    30361 tccggcagcg gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc
    30421 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    30481 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    30541 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    30601 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg
    30661 tctaccgcac cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    30721 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    30781 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    30841 tacgcctcgg gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact
    30901 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    30961 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    31021 gcctgactta atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg
    31081 cggggcattc acggacctca taatcaactt tattttctgg acagattcaa caggttaacg
    31141 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    31201 tcagtccgtg actggtgcgc atcataaagt aagcagatag attgcgcaat ttttatacaa
    31261 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    31321 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    31381 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    31441 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    31501 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc
    31561 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    31621 accgtctttg cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag
    31681 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    31741 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    31801 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    31861 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    31921 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    31981 taactgctga ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg
    32041 gatttcgctg tcttcagtat ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    32101 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    32161 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    32221 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    32281 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    32341 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    32401 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    32461 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    32521 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    32581 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    32641 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    32701 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    32761 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    32821 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    32881 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    32941 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    33001 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    33061 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    33121 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    33181 gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa
    33241 gcgactgtca ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg
    33301 cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    33361 tgcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    33421 agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc
    33481 acgctgcggg ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg
    33541 tttgttatcg ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag
    33601 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc
    33661 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    33721 cccccatgag gccagcagcg agttgccgac atcctgttca ccatcgctgt taaagagctg
    33781 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    33841 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    33901 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    33961 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    34021 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    34081 ccagcgcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg
    34141 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    34201 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    34261 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    34321 ctccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    34381 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    34441 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    34501 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    34561 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    34621 gacccttaac atagcggctc ctgactactg acaattcggg caatgcaggc gggtcatctc
    34681 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    34741 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    34801 ctgccaacca tgccgccaga gcttttggta ctggtttctg ctggcgagac tgttcattga
    34861 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcagcaccat
    34921 cagcaccatc agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt
    34981 cagggaagct gacataatgc cccctctttc agcggacaaa aatcgctcca gccgcgtggc
    35041 gaaaaaacaa tttttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa
    35101 ccggcaatca ataaggcttc attatctgcc agtaaacgca aacaaacctg ggcatcggtt
    35161 ccagcgtact gcgagcactg aactgctggc tgcgtctgcc aggagtgcat ttttccccac
    35221 gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttccat gctcacgcga
    35281 gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc
    35341 agcatcagga ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg
    35401 tcacgctata gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat
    35461 taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga
    35521 taatgatggc atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac
    35581 cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtcg gactctttta
    35641 ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac
    35701 cctgtccgac aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct
    35761 ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagt gccagcttac
    35821 gagtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca
    35881 atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta
    35941 caggcatcct tctgtttctc cttgctgata aagccggagt cttccccacg gcgaaaccac
    36001 cagccaccac tcgcccgttg agtttttgaa gcgaatatgc acggcccatg cggtattgcg
    36061 caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg
    36121 cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag
    36181 gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc
    36241 atcttcacgt aaatagagca aatagtcccg cgcctagctg gcggtttgcc aaagccgttg
    36301 cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag
    36361 catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg
    36421 gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta
    36481 tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag
    36541 ggtaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc
    36601 caccggcgct ttaacgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg
    36661 gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc
    36721 gcggcttaat tgcagatgag tttgatccat atggttgctg tacaggtgcg tatcgccacc
    36781 ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa
    36841 cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag
    36901 ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag
    36961 cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg
    37021 gtcgtttttc agctggttca gtaccgtagt aatctggtca atatgacgac catctggcgt
    37081 tggccaggcg cgccactgtt taccatacac tggcccgagg tcgccatttt catcggccca
    37141 ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtat cgccctgcag
    37201 gaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttggttg tcaccagcgg
    37261 gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc
    37321 ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg
    37381 tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat
    37441 gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac
    37501 ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc
    37561 gccgtagcca atcaggaaca agcctgagac agctcccatt gggcgcggtt tacgaatata
    37621 caggttgagg ataataaaca gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg
    37681 gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag
    37741 cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc
    37801 aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa
    37861 cggaatgagt ggtgcgataa aatcagagac ctggaagaag gaacgtttag tacggcgggc
    37921 gaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc
    37981 ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac
    38041 ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac
    38101 ttcatttttg gtccagccac tgcccggacg attcgcccga cgtgttgcca gccacattgc
    38161 aaaaatgaac cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga
    38221 gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa
    38281 gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg
    38341 ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag
    38401 atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc
    38461 cataaaggct gcaacctgat gacgaacttc ggtcgccagt tgcgcttcca gactacgctg
    38521 cgcaagattt tctgcttcgg caaaatcaat gcgccgcagc aggtatttta cccgcgctac
    38581 agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat
    38641 gggatcgccc gccatttcac cgcacaaacg aagatcgata ccatgtattt ccgcttcccg
    38701 ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac
    38761 ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga
    38821 gatgaaatcg accctctttg ccagatgcgg cagcataaat accattgacg gcacttccag
    38881 catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc
    38941 ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa
    39001 cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa
    39061 gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc
    39121 ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt
    39181 gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc
    39241 cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc
    39301 aatacagctg cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt
    39361 tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct
    39421 aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc
    39481 gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg
    39541 ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg
    39601 ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc
    39661 aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg
    39721 cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcacc
    39781 agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat
    39841 gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg
    39901 acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt
    39961 ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag
    40021 cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg
    40081 ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc
    40141 cggtaatgcg cggattcgcg tctggcgata ttgcccaaac agggcagtca actgcgactg
    40201 agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc
    40261 atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat
    40321 aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa
    40381 gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc
    40441 gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg
    40501 ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc
    40561 ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg
    40621 tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta
    40681 acctctttta cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga
    40741 catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac
    40801 cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact
    40861 gctggtttgc atattgattt ctgcatcgcc gcttaccagc tgcaagagaa accatttttg
    40921 tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta
    40981 gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc
    41041 ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg
    41101 cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt
    41161 acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca
    41221 aaataaagct ttatatacga atgattgttt catactccag gaagacggta aaccactctc
    41281 tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa
    41341 cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa
    41401 ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat agacatttta
    41461 aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg
    41521 tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat
    41581 ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg
    41641 tcaaggctgt aaatgaaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg
    41701 ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt
    41761 gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca
    41821 catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca
    41881 aggtatcatg acatgtccca acctcgccca ctgctctctc ctcccgaaac tgaagaacag
    41941 ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc ggcacttgac
    42001 gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag
    42061 atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg
    42121 gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt
    42181 gttgcccggt taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag
    42241 ctcaaacgcg tactgtggat accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt
    42301 cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa
    42361 gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt
    42421 cacggggaat atttacagat aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc
    42481 attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat
    42541 ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc
    42601 gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg
    42661 catggataac cgatcctaac gcttggcttg cgctcggtac gctgacgctg ctggagatcg
    42721 ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctt cccacagcac
    42781 aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc
    42841 tggcatcaat ctcttgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg
    42901 aaatttctgc ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca
    42961 gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat
    43021 cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg
    43081 tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggcagcc gtggtgattg
    43141 ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt
    43201 cggtaaaaat gctggcgctc tctttcctga ttctggtggg ctttaccctg attctggaaa
    43261 gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg
    43321 ttgaaagcct caacttgatt cgcaacaaaa agaatccgct ctgatactcc gtacgctctc
    43381 ctgcgggagg gcgtactcac tccttttacc ctcacagttt aagattttcc tgctttcaag
    43441 atatataacg tcggtttata aacagactat tatcataggt aggccaaatg aggataaccg
    43501 gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg
    43561 ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc
    43621 tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca
    43681 aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg
    43741 ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt atattcctta
    43801 tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca cagttcgctg
    43861 gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag cgaagccgac
    43921 gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga cgttgccgaa
    43981 atgtacccag tacctccgcg ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac
    44041 tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg
    44101 tcgcgcaata atgacaaggg catccgcccg gatcaggcgc tggatcggaa gaatatccgc
    44161 gaagcgctgc atgacagcct caagcgccta cagactgatt acctcgatct ttatcaggtg
    44221 cactggccgc agcgcccgac caactgcttc ggcaaactcg gttatagctg gacggattct
    44281 gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg cagagtacca acgcgcggga
    44341 aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat
    44401 ctggcggaca aacacgatct gccgcgtatt gtcaccattc agaaccctta cagtctgtta
    44461 aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaaggggt cgaactgctg
    44521 gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaatgg tgcaaaaccc
    44581 gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa
    44641 aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg gccttgatcc tgctcagatg
    44701 gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca ctctgctggg cgcaaccacg
    44761 atggatcagc tgaaaactaa catcgaaagt ttgcatctgg agttaagcga agacgtatta
    44821 gctgaaattg aagcggtaca tcaggtttat acttatcctg caccataaag attaacgccg
    44881 gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc cgttattgcc
    44941 agcgcaaata gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc taccattacc
    45001 gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag gttttgtact
    45061 gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa cgcattgagc
    45121 ggaacgacaa aaaagccccc cagcacgcca atcagcatca acaaggcata ggctggcagc
    45181 agctcgtgtt gcagggagaa aatcggtacc accacgccaa tcaaaatccc ggctggcata
    45241 cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata
    45301 ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc
    45361 accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca gaataaactg
    45421 gtgcccacca gcgaaaaacg cgtttcacca ttgcaccata gcgaggtgca ggcattcagg
    45481 aaactgcggg tcatgttgat gagattccag gactgccccg gacgcgccgc cagtttggga
    45541 atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc cagagcgacc
    45601 aggacatgcc agtcagccag cacaccaccg gctacggaac cgagtaaaat cgccgctatg
    45661 gcagaagctt ccattaaacc gttagctttt actaacttac tacccgtggt taattcgccg
    45721 agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag cgtatagccg
    45781 agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag gccgttggca
    45841 aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa
    45901 agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc cggatagaac
    45961 tgcgctttca gtaacgccag agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga
    46021 aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact
    46081 gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg
    46141 tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg gtacagcaag
    46201 ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca gttcgttatc
    46261 tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg ctcttaatcg cagtggcatg
    46321 gactttatct ggcgaaacac caagtgccag ttgttccacc atttccagcg acaccatttc
    46381 gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc
    46441 aaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc gaacattctc
    46501 ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt tcattatgtt
    46561 cggccctttc agttgcaggc gtccgccctc ttcgatacca gggaccgaca acaggcgtgc
    46621 atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg gtacgttgat
    46681 agaaacgaca ggcgcgcatt cggtcacacc gtagccttca aggatgcgca ggccaaattt
    46741 atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca ccacatagcg
    46801 tagacgatag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt
    46861 accgaacaac acggtgcaac tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag
    46921 cgggcttgga taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt
    46981 cagcccaaag gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc
    47041 ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt ttatggctat ggacgacgcc
    47101 ttttggatgg ccttcagaac cggaggtaaa gaggatcagc gcctcttctt ccggctgctg
    47161 ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag atccatactt tgtcggcagt
    47221 ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa gttgctccgg
    47281 cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc
    47341 agccgccgta atagcactgg tcagcccttt taccccggca gtgtagttca tcattgcggg
    47401 aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg cattgggcag
    47461 cattaagccg atgcgttcgc cttcaacact gtatttttca aggatgcgtc caacaaacag
    47521 cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt cgacacattt
    47581 cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagag tttcacgcgg
    47641 gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc atttcgccag cgattttgcg
    47701 acggtcacgg gcacgcggcg catccggcat cgccacctgc gttggaggca aaatatgcag
    47761 agtaatttgc gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtaag
    47821 ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaaacc
    47881 cgcgccatcg tagattttca tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat
    47941 caccactggt cggccctgtt ccaccagacg taccagatgt ttaatagcca taggttgcgt
    48001 cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct
    48061 tattgaggtg taaacggcaa acactggacg cacaggtaaa aacagtccaa gcaaaatgcc
    48121 atcaataaaa gagacgtgat taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt
    48181 gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag
    48241 cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata
    48301 aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg
    48361 tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat
    48421 cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga
    48481 aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa
    48541 acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acacgaagtt gacttcacct
    48601 atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat
    48661 cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta
    48721 tttgcgtttg gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt
    48781 ttccgtaaca ctgaaacaat gtaagcgttt acccactaag gtattttcat ggcgaccata
    48841 aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat
    48901 tcacccaaag ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc
    48961 tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg
    49021 atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg
    49081 gcttatcaca ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag
    49141 cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa
    49201 atgatcccag atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc
    49261 aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc
    49321 tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct
    49381 aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa
    49441 agtggtattc cggccaatga tcggctggtg acatttggcg aaccagacga aagcggcggc
    49501 gaacaggcaa tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat
    49561 aacgattcaa tggcggcggg cgcgatgggc gttctcaatg ataatggtat tgatgtaccg
    49621 ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc
    49681 ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc aggctgccga actggctttg
    49741 gcactggcgg ataatcgccc tctcccggaa attactaatg tatttagtcc gacgctggta
    49801 cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa
    49861 ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa
    49921 ccgcgcctga ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga
    49981 tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac
    50041 ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga
    50101 ttcacataac ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc
    50161 atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc
    50221 gttgaacccg gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac
    50281 ctgagtaatt aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt
    50341 cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata
    50401 atgttcggta tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc
    50461 agaaatagcc tgtaaatcct gaccgaattc gataacctga cgcaccatag caccacacac
    50521 ctgctccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc cgactagctg
    50581 cagatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg
    50641 cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt
    50701 aacgcgcagc catacccgat gccctggcga aacctggccc agttggtcga gcgtatcaac
    50761 agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat
    50821 aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa
    50881 cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa
    50941 gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccactt caaactgttt
    51001 cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa
    51061 ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga
    51121 atgtggcata acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga
    51181 ataaaaaata tcgatttatg tcgagtctat gcaaaaatga tatggattac cggattgcga
    51241 gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac
    51301 cgccggaagc ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg
    51361 cgaactggcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg
    51421 attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg
    51481 actggatcgc attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc
    51541 tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct
    51601 ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga
    51661 gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata cgcctacggg
    51721 aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc
    51781 gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaatt acatcagcct
    51841 ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa
    51901 acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg
    51961 ggtaggtatt tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt
    52021 ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg
    52081 cccgtcatca gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact
    52141 ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc
    52201 tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt
    52261 aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca
    52321 atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca
    52381 aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa
    52441 aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc
    52501 agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt
    52561 aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta
    52621 cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg
    52681 tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg
    52741 ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt
    52801 atcagacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt
    52861 ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac
    52921 gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat
    52981 ccggcccgtg aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt
    53041 cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagta atacccacaa
    53101 acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca
    53161 gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg
    53221 taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg
    53281 gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg
    53341 acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag
    53401 tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta
    53461 ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa
    53521 tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca
    53581 tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag
    53641 cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc
    53701 tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca
    53761 acacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca
    53821 ggaagaccac cagaataatc agcagaactg ctggtaaagc aagaaccccc aacattgcgc
    53881 gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg
    53941 tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag
    54001 aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc accacacgag
    54061 cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca
    54121 ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc caaccattaa
    54181 acagcgcacc aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac
    54241 tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa
    54301 gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg
    54361 tatcacgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct
    54421 gccagcagag agtaagacat tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc
    54481 ggaaacagca taaatatgga ttaaattgct gcgacatgcc gttatgtgat ggatattcca
    54541 attttaaaat taagttgaat tattgagatt attattaacc tcctaatttt aaagcagata
    54601 aaattcataa agttcattaa ttgataattg atatggatta tttcataacc atgatatgga
    54661 ttatgatgat ctagagatat aaaaaaccct gccatgcggc agggtcataa aagtaagagg
    54721 aatgaattaa cgcgccagcc aaccgccatc cacagcaatg gtataaccat ttacataatc
    54781 tgaagcgctg gaggcaagaa acactaccgg ccccatcagg tcactcggca gtccccaacg
    54841 gccagctgga atgcgatcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg
    54901 ggtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
    54961 ccattcgttt gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga
    55021 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
    55081 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
    55141 attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg
    55201 aatcaatccg gcgttattca ccaggatatc aatatgacca aattcagcta ccgcgcgatc
    55261 cagcagtcca ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
    55321 ccccagcgcc gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat
    55381 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc
    55441 agtgacgacc gcaactttac cttggagaga aaatgcactt aaaatcataa caacacctta
    55501 ttcgtttatg cccacaacta gcgcaaatct ttaacgccca catggtccat atcattaaag
    55561 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
    55621 tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt
    55681 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
    55741 taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacctgga
    55801 gccagctccg tcagccccat actcaattga caggtttcca gtacatccgg gacaaaatat
    55861 ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
    55921 tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
    55981 ttcgccggag tggcggtatc aatactggca aagacaactt cttttgcacc tttaccaaca
    56041 tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca
    56101 ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt
    56161 ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata
    56221 attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc
    56281 aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc
    56341 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt
    56401 gaaacactgt ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat
    56461 taaaaataaa acaatgtttt atttttaatt taaggattaa aaaaagtgca gtgtcgatca
    56521 cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag
    56581 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag
    56641 ctactcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc
    56701 cagccctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca
    56761 accggaagcg cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg
    56821 ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
    56881 ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc
    56941 atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat
    57001 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc
    57061 agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc acagaaccga gactatcaaa
    57121 tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt
    57181 atcaacaacc aacgtctgac cgttactttg gatcattacc gggacgatct catctttaaa
    57241 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
    57301 caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
    57361 atcattgaag gcatcccaca acccatcatg caccagactg tcaaccaact ggctattgcc
    57421 aagctgttca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
    57481 gccacctgcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
    57541 tgctttaaga ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag
    57601 accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg
    57661 accaagaatt acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt
    57721 cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccac gaaagcagcc
    57781 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
    57841 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat
    57901 ttttatcaca aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt
    57961 tgcaaccact aacccaccaa tagatgggta gatagggcgt taatctccca tacttaactt
    58021 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
    58081 ctaatatttg gtctaaagaa gaaactctgt gaagtttcgc gctctatggc acagccgttg
    58141 atgcaggcac gctattcctt cctattcagt taggttcggc aggggctgtg gtcctgttta
    58201 ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca
    58261 tcctctcatc aaaaacatca gcaggtgaag ggataacggg cgcggtaaca cactactatg
    58321 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
    58381 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
    58441 ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaattctg aatctcattt
    58501 ttctgatggg acgtcatgcc actattcggg taatgggatt tttggtattc ccattgattg
    58561 cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacaa
    58621 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
    58681 ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
    58741 atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
    58801 gcataagtgt actgttcttt gtctttagct gcctgctttc tattccatct tcgtatattg
    58861 aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc
    58921 cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
    58981 tgggtacgta ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca acactacagc
    59041 aggttggtgt aaagaaaagt cgtgcattta accacgcact atcaattatg ttggtatcgc
    59101 tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca
    59161 gcggcccgct cattgccatg atacttttca tcatgcctac gctgtcaacg tatctcatcc
    59221 cggcgcttaa accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt
    59281 gcgtatcggt aatgttcttt agctaatatc cgttgtggat ggttcaaaat tgctacaatt
    59341 ttgaaccatt ccatttttca taatattcat ttgttattat tccgccctat cacttctctc
    59401 gtcatatcct agaattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa
    59461 gactatttaa gatgcataac ttagattcgc aagatatctt ctggtcattc aaaaacaatt
    59521 tgcgatatag atattctcat accatgagat agttatctgg accacgttcg accatgcaag
    59581 attaaatcag tgaaacataa tcatatttaa taacgagaaa tgcattttta aatatcttaa
    59641 tcagaattat agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcaag
    59701 gtgtattgac atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc
    59761 ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt
    59821 gccaaataat gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg tgttttgatc
    59881 ttacccagca atagtggaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
    59941 atgacaaaaa cagtatcaac cagtaaaaaa ccccgtaaac agcattcgcc tgaatttcgc
    60001 agtgaagccc tgaagcttgc tgaacgcatc ggtgttactg ccgcagcccg tgaactcagc
    60061 ctgtatgaat cacagctcta caactggcgc agtaaacagc aaaatcagca gacgtcttct
    60121 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
    60181 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
    60241 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
    60301 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
    60361 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
    60421 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
    60481 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
    60541 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaa
//
LOCUS       DAJTRM010000012        60409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRM010000012 DAJTRM010000000
VERSION     DAJTRM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.012"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..312
                     /locus_tag="OO322_000812"
     CDS             <1..312
                     /locus_tag="OO322_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5905792.1"
                     /translation="TENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNS
                     VYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTY
                     P"
     gene            425..1219
                     /gene="panB"
                     /locus_tag="OO322_000813"
     CDS             425..1219
                     /gene="panB"
                     /locus_tag="OO322_000813"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5905793.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1231..2082
                     /gene="panC"
                     /locus_tag="OO322_000814"
     CDS             1231..2082
                     /gene="panC"
                     /locus_tag="OO322_000814"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5905794.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2156..3058)
                     /gene="rpnC"
                     /locus_tag="OO322_000815"
     CDS             complement(2156..3058)
                     /gene="rpnC"
                     /locus_tag="OO322_000815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5905795.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3332..3712
                     /gene="panD"
                     /locus_tag="OO322_000816"
     CDS             3332..3712
                     /gene="panD"
                     /locus_tag="OO322_000816"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5905796.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3716..4945)
                     /locus_tag="OO322_000817"
     CDS             complement(3716..4945)
                     /locus_tag="OO322_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5905797.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5009..5449)
                     /locus_tag="OO322_000818"
     CDS             complement(5009..5449)
                     /locus_tag="OO322_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5905798.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5554..6324)
                     /locus_tag="OO322_000819"
     CDS             complement(5554..6324)
                     /locus_tag="OO322_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5905799.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6321..7247)
                     /locus_tag="OO322_000820"
     CDS             complement(6321..7247)
                     /locus_tag="OO322_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5905800.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7356..8018
                     /gene="can"
                     /locus_tag="OO322_000821"
     CDS             7356..8018
                     /gene="can"
                     /locus_tag="OO322_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5905801.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8059..8595)
                     /gene="hpt"
                     /locus_tag="OO322_000822"
     CDS             complement(8059..8595)
                     /gene="hpt"
                     /locus_tag="OO322_000822"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5905802.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8801..11191
                     /gene="gcd"
                     /locus_tag="OO322_000823"
     CDS             8801..11191
                     /gene="gcd"
                     /locus_tag="OO322_000823"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5905803.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11238..12788)
                     /gene="cueO"
                     /locus_tag="OO322_000824"
     CDS             complement(11238..12788)
                     /gene="cueO"
                     /locus_tag="OO322_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5905804.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12954..13301
                     /locus_tag="OO322_000825"
     CDS             12954..13301
                     /locus_tag="OO322_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5905805.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13407..14273
                     /gene="speE"
                     /locus_tag="OO322_000826"
     CDS             13407..14273
                     /gene="speE"
                     /locus_tag="OO322_000826"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5905806.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14289..15083
                     /gene="speD"
                     /locus_tag="OO322_000827"
     CDS             14289..15083
                     /gene="speD"
                     /locus_tag="OO322_000827"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5905807.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15121..15483)
                     /locus_tag="OO322_000828"
     CDS             complement(15121..15483)
                     /locus_tag="OO322_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5905808.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15658..18255)
                     /gene="acnB"
                     /locus_tag="OO322_000829"
     CDS             complement(15658..18255)
                     /gene="acnB"
                     /locus_tag="OO322_000829"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5905809.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18610..20463
                     /locus_tag="OO322_000830"
                     /pseudo
     CDS             18610..20463
                     /locus_tag="OO322_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20534..21958)
                     /gene="lpdA"
                     /locus_tag="OO322_000831"
     CDS             complement(20534..21958)
                     /gene="lpdA"
                     /locus_tag="OO322_000831"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5905810.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22283..24163)
                     /gene="aceF"
                     /locus_tag="OO322_000832"
     CDS             complement(22283..24163)
                     /gene="aceF"
                     /locus_tag="OO322_000832"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5905811.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24178..26841)
                     /gene="aceE"
                     /locus_tag="OO322_000833"
     CDS             complement(24178..26841)
                     /gene="aceE"
                     /locus_tag="OO322_000833"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5905812.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27002..27766)
                     /gene="pdhR"
                     /locus_tag="OO322_000834"
     CDS             complement(27002..27766)
                     /gene="pdhR"
                     /locus_tag="OO322_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5905813.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28307..29680
                     /gene="aroP"
                     /locus_tag="OO322_000835"
     CDS             28307..29680
                     /gene="aroP"
                     /locus_tag="OO322_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5905814.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29723..30577)
                     /gene="ampE"
                     /locus_tag="OO322_000836"
     CDS             complement(29723..30577)
                     /gene="ampE"
                     /locus_tag="OO322_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5905815.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30574..31125)
                     /gene="ampD"
                     /locus_tag="OO322_000837"
     CDS             complement(30574..31125)
                     /gene="ampD"
                     /locus_tag="OO322_000837"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5905816.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31213..32106
                     /gene="nadC"
                     /locus_tag="OO322_000838"
     CDS             31213..32106
                     /gene="nadC"
                     /locus_tag="OO322_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5905817.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32309..32749
                     /gene="ppdD"
                     /locus_tag="OO322_000839"
     CDS             32309..32749
                     /gene="ppdD"
                     /locus_tag="OO322_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5905818.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32759..33906
                     /gene="tadA"
                     /locus_tag="OO322_000840"
                     /pseudo
     CDS             32759..33906
                     /gene="tadA"
                     /locus_tag="OO322_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33896..35098
                     /gene="hofC"
                     /locus_tag="OO322_000841"
     CDS             33896..35098
                     /gene="hofC"
                     /locus_tag="OO322_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5905819.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35133..36176)
                     /gene="guaC"
                     /locus_tag="OO322_000842"
     CDS             complement(35133..36176)
                     /gene="guaC"
                     /locus_tag="OO322_000842"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5905820.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36338..36376)
                     /locus_tag="OO322_000843"
     CDS             complement(36338..36376)
                     /locus_tag="OO322_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905821.1"
                     /translation="MIIKTLKMSARK"
     gene            36401..37021
                     /gene="coaE"
                     /locus_tag="OO322_000844"
     CDS             36401..37021
                     /gene="coaE"
                     /locus_tag="OO322_000844"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5905822.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37021..37764
                     /gene="zapD"
                     /locus_tag="OO322_000845"
     CDS             37021..37764
                     /gene="zapD"
                     /locus_tag="OO322_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5905823.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37774..37971
                     /gene="yacG"
                     /locus_tag="OO322_000846"
     CDS             37774..37971
                     /gene="yacG"
                     /locus_tag="OO322_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5905824.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38071..38460)
                     /gene="mutT"
                     /locus_tag="OO322_000847"
     CDS             complement(38071..38460)
                     /gene="mutT"
                     /locus_tag="OO322_000847"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5905825.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38520..41225)
                     /gene="secA"
                     /locus_tag="OO322_000848"
     CDS             complement(38520..41225)
                     /gene="secA"
                     /locus_tag="OO322_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5905826.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41287..41799)
                     /gene="secM"
                     /locus_tag="OO322_000849"
     CDS             complement(41287..41799)
                     /gene="secM"
                     /locus_tag="OO322_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5905827.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42030..42947)
                     /gene="lpxC"
                     /locus_tag="OO322_000850"
     CDS             complement(42030..42947)
                     /gene="lpxC"
                     /locus_tag="OO322_000850"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5905828.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43048..44199)
                     /gene="ftsZ"
                     /locus_tag="OO322_000851"
     CDS             complement(43048..44199)
                     /gene="ftsZ"
                     /locus_tag="OO322_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5905829.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44260..45522)
                     /gene="ftsA"
                     /locus_tag="OO322_000852"
     CDS             complement(44260..45522)
                     /gene="ftsA"
                     /locus_tag="OO322_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5905830.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45519..46349)
                     /gene="ftsQ"
                     /locus_tag="OO322_000853"
     CDS             complement(45519..46349)
                     /gene="ftsQ"
                     /locus_tag="OO322_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5905831.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46351..47271)
                     /gene="ddlB"
                     /locus_tag="OO322_000854"
     CDS             complement(46351..47271)
                     /gene="ddlB"
                     /locus_tag="OO322_000854"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5905832.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47264..48739)
                     /gene="murC"
                     /locus_tag="OO322_000855"
     CDS             complement(47264..48739)
                     /gene="murC"
                     /locus_tag="OO322_000855"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5905833.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48793..49860)
                     /gene="murG"
                     /locus_tag="OO322_000856"
     CDS             complement(48793..49860)
                     /gene="murG"
                     /locus_tag="OO322_000856"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5905834.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49857..51101)
                     /gene="ftsW"
                     /locus_tag="OO322_000857"
     CDS             complement(49857..51101)
                     /gene="ftsW"
                     /locus_tag="OO322_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5905835.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51101..52417)
                     /gene="murD"
                     /locus_tag="OO322_000858"
     CDS             complement(51101..52417)
                     /gene="murD"
                     /locus_tag="OO322_000858"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5905836.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52420..53502)
                     /gene="mraY"
                     /locus_tag="OO322_000859"
     CDS             complement(52420..53502)
                     /gene="mraY"
                     /locus_tag="OO322_000859"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5905837.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53496..54854)
                     /gene="murF"
                     /locus_tag="OO322_000860"
     CDS             complement(53496..54854)
                     /gene="murF"
                     /locus_tag="OO322_000860"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5905838.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54851..56338)
                     /gene="murE"
                     /locus_tag="OO322_000861"
     CDS             complement(54851..56338)
                     /gene="murE"
                     /locus_tag="OO322_000861"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5905839.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56325..58091)
                     /gene="ftsI"
                     /locus_tag="OO322_000862"
     CDS             complement(56325..58091)
                     /gene="ftsI"
                     /locus_tag="OO322_000862"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5905840.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58107..58472)
                     /gene="ftsL"
                     /locus_tag="OO322_000863"
     CDS             complement(58107..58472)
                     /gene="ftsL"
                     /locus_tag="OO322_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5905841.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58469..59410)
                     /gene="rsmH"
                     /locus_tag="OO322_000864"
     CDS             complement(58469..59410)
                     /gene="rsmH"
                     /locus_tag="OO322_000864"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5905842.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59412..59870)
                     /gene="mraZ"
                     /locus_tag="OO322_000865"
     CDS             complement(59412..59870)
                     /gene="mraZ"
                     /locus_tag="OO322_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5905843.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14471 a        16732 c        14843 g        14363 t
ORIGIN      
        1 actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc aaaaggagtc
       61 ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt gaaacctaat
      121 gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac aacaggagga
      181 gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc cacgctacaa
      241 caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac tttccaggta
      301 acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa ttgataaatc
      361 attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt tatcaggata
      421 cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata aaaagcgttt
      481 cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg aagggcttaa
      541 cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt ccactctgcc
      601 agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg caccaaactg
      661 cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag cctttgaaaa
      721 cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg gtgagtggct
      781 ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc acttaggttt
      841 aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg gcaatgaagc
      901 gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac agctgctggt
      961 gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg cgatcccggt
     1021 tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc acgacgcctt
     1081 tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg aaacgggcga
     1141 catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt atccgggcga
     1201 agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct gccgctgctg
     1261 cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt gcctactatg
     1321 ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg cgccgatgtg
     1381 gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga tctggcacgt
     1441 tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt ggatttagtt
     1501 ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac ttacgttgac
     1561 gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt tcgcggcgtt
     1621 tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg cttcggtgaa
     1681 aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg cttcgatatt
     1741 gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag ttcccgtaac
     1801 ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt tttaagttcg
     1861 attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac cattgcgggg
     1921 caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga tgccgacaca
     1981 ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc ctggcttggc
     2041 gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac tggcgctacg
     2101 gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag gtaacttaaa
     2161 taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct gcaacgtctt
     2221 cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc tcctgtcttc
     2281 cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc gtcatcatag
     2341 acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct tgttcagtat
     2401 gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta gtgtaccctt
     2461 cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg cgaatatgtt
     2521 tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca tccggtgtga
     2581 tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc tccggcgagt
     2641 aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc tgataaaaca
     2701 gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg tgcatggcag
     2761 caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt tggtgttcaa
     2821 tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc acgtccgtgc
     2881 tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc gtgttgagat
     2941 cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga gccgtctccg
     3001 catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt gcatccatct
     3061 tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt gctatcgtca
     3121 gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt tacaggcgtt
     3181 acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca gatgagtaga
     3241 cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc accgcctgct
     3301 tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc agggcaaact
     3361 ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg ccattgacca
     3421 ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct ggaatgtcac
     3481 caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga gaattatttc
     3541 tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca tcgccagctt
     3601 cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct attttgaagg
     3661 cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt gattgttatc
     3721 cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt cttaagatat
     3781 aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc atggttgtca
     3841 ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta aagccgccaa
     3901 acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg cgtgaacgtg
     3961 caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg cgataaccat
     4021 ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga atttcgttaa
     4081 gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg tgacgtttga
     4141 tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac acaggatacg
     4201 catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct cgcgcaggga
     4261 gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc gcgtatcccc
     4321 tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc gtcgaagtat
     4381 ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc accggcaggc
     4441 cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca atacggatct
     4501 gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag cgcaaattgt
     4561 ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc ggcgtatctt
     4621 tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa ccgtcttcaa
     4681 ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct ttaccaaagc
     4741 caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt tgtccggccc
     4801 gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca gcattttcga
     4861 tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac agcatcagca
     4921 ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa cgcttttatt
     4981 gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt ttgaggacga
     5041 aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca cccagttcga
     5101 caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc atctgttcaa
     5161 ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg cttaataatg
     5221 aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca ccctcaccag
     5281 agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag cttaatccgc
     5341 gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc agcgcatcca
     5401 gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc gccttcctcc
     5461 ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc gtcaaaactc
     5521 ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac gtccacgttg
     5581 gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta cgccaaaggt
     5641 agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac tgatcatata
     5701 aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag tcagtgagta
     5761 aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca ggctgatgtc
     5821 atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata gcaccgccgt
     5881 gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca caaaaaacag
     5941 tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac cgccgacata
     6001 tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa tattacgctg
     6061 gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga tcaccgacat
     6121 catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat cgccaatacg
     6181 cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga ctggcggcac
     6241 cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga tgctttttag
     6301 cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac cagtgaaaca
     6361 aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat gccctgctca
     6421 cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac ttccagcgtc
     6481 gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact tttcggtgcg
     6541 agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat cgaggtattt
     6601 tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc tgcttcttcc
     6661 aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt caaaaagccc
     6721 cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag aatcagtagt
     6781 ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc gccagataac
     6841 atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata tttttcgctg
     6901 cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac cacaatttgc
     6961 tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa ctgacgttta
     7021 gcattcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc ggaggtttta
     7081 tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt cggcccgaga
     7141 agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc ctgaacgccg
     7201 cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa tttttactta
     7261 ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc tatattagcc
     7321 aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat acactcatca
     7381 gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt tttgagaaac
     7441 tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt cgcgttcctg
     7501 cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat gttgctaacc
     7561 tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg gatgtactcg
     7621 aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa gccgccgttg
     7681 aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat atctggttca
     7741 aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc ttgtgtgaac
     7801 tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa tcagcgtgga
     7861 aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac ggcttgctgc
     7921 gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac cgtcacggga
     7981 tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc ggatgcaaca
     8041 catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat acggcagatg
     8101 acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg ggatcgagaa
     8161 accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca gcagcgtaca
     8221 aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg acagtgtatt
     8281 ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat cttcatccag
     8341 atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc tggaggcggt
     8401 cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca taaacataaa
     8461 tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt aacgctcagt
     8521 aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga tcattacttc
     8581 tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc gcacgggcag
     8641 gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta ttcattcgcg
     8701 gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac ctataattaa
     8761 ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta acaatacagc
     8821 ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg ggctgtatct
     8881 actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc ctatcgctgg
     8941 ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg cgctttggct
     9001 atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg gtttcgactt
     9061 ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc tgatcctgcc
     9121 gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac tggtggtcgc
     9181 actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc aggagatcaa
     9241 cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg ccgatcagga
     9301 ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga agcaaattca
     9361 cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg atgtgaagca
     9421 gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg gcgataccct
     9481 ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg gcaaagagaa
     9541 atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa cctgccgtgg
     9601 cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg cggattgccc
     9661 gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg ctgaaaacgg
     9721 caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca atatgccaga
     9781 caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata aaaccatcgt
     9841 gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg tgatccgtgg
     9901 ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga aagatccgaa
     9961 cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt gggcaccagc
    10021 ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca cgccggatat
    10081 ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc tggcgctgaa
    10141 tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc tgtgggacat
    10201 ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga aagtgccgat
    10261 tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta atggcgaact
    10321 ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg attacgttac
    10381 ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga gcggtgcgga
    10441 tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc accagatgcg
    10501 ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc cgggtaacct
    10561 ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg caattgccaa
    10621 cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca acccgatgga
    10681 gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac agtacggtgt
    10741 accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat gtaaacagcc
    10801 agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt ggaagaaacg
    10861 tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc cgttcaatat
    10921 gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt ttatcgccgc
    10981 tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac tgtggcaggg
    11041 tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg gcaagcagta
    11101 cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg actatattgt
    11161 ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat ccgggcatat
    11221 ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc ttcatgctcc
    11281 agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt aaacttcacc
    11341 agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc gcgatgagcc
    11401 gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat atggaacgga
    11461 tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc tttcgccgcc
    11521 gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc atggtggaaa
    11581 tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg gcccatcatc
    11641 tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag catctgcatc
    11701 cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt cagcccttcc
    11761 agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga ggcactaata
    11821 gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat caccatcccc
    11881 atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac ctccaccagc
    11941 acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg ttcaggtagc
    12001 agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc gaaattgagc
    12061 gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg ggcagcgtgt
    12121 tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac ggcggcggtc
    12181 atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc ctgaacgatc
    12241 accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat ctcgtcatct
    12301 tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt gccatgttga
    12361 tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac cgagcgcttg
    12421 ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac ttccagcccg
    12481 tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt taccgctttg
    12541 ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca ggtagttgca
    12601 gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg attacgggca
    12661 tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa gactgcacgg
    12721 ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa gaaatcacga
    12781 cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg ttgagcatag
    12841 tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat aatggtcgtc
    12901 gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa gcaatgaaga
    12961 cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac agttacgcgc
    13021 tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc tttctgaaga
    13081 acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg gtctttttcg
    13141 cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa gctctcggtg
    13201 aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa tgcaaagccc
    13261 tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata cctttttgta
    13321 gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc tttttttacg
    13381 ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat gaaacgctac
    13441 acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa aagaccgatc
    13501 accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg ctggatggcg
    13561 tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc catgttccgc
    13621 tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac ggtgccatgc
    13681 tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa atcgatgcgg
    13741 gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc tacgacgatc
    13801 cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc agccagacct
    13861 ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc ctgttcacct
    13921 cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc gtcgcacaaa
    13981 acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa ctcagccatt
    14041 acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc ggtatcatga
    14101 cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa attattcagg
    14161 cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc catacggcag
    14221 cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc taaggagaag
    14281 ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa agtctgagtt
    14341 tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt tatattgctt
    14401 atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa acctgttcca
    14461 ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag ggtgccagcg
    14521 tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa acagaacacc
    14581 ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt tgcgtacata
    14641 cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat attgaagtct
    14701 ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac cagcttgagt
    14761 ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt aacggtatga
    14821 agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac gatatgaagg
    14881 cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat accaagatgt
    14941 tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa gatttaaccg
    15001 acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag atttattacg
    15061 ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg ccagatttat
    15121 ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat cctcaacgcc
    15181 gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt catcgccagc
    15241 gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca gggtgtattc
    15301 atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg cttgttccac
    15361 ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc cgaccacttc
    15421 atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa attcgtaatc
    15481 catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca ttgatgtaat
    15541 tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat aaaaaatgcc
    15601 cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc ttacttatta
    15661 caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa gctggttgaa
    15721 gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt aggtctggta
    15781 ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt ctgcagaagc
    15841 caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag aggtggaaac
    15901 caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac agcccgggat
    15961 ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg tcagttgcgc
    16021 ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac ctttgtgcgc
    16081 atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag aaccgataaa
    16141 tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat ccggatcgtt
    16201 cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca ctgccgcgta
    16261 ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt ccatgccctg
    16321 aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc acttcagcag
    16381 gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga tagtacaacc
    16441 agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa ctttcagatc
    16501 cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc ctttcttctc
    16561 aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca gatcgcgcag
    16621 ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg gcatatccag
    16681 cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg ggaaagagat
    16741 accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg gcagcagcat
    16801 acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac cgccacggtt
    16861 cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg gatacgccgc
    16921 ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg ccaggtcttt
    16981 cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag tcattttcgg
    17041 ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta ccattttctg
    17101 cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga acacatcact
    17161 gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga taatcagcgg
    17221 aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt cgaaggtcgc
    17281 cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa cgtcaatcac
    17341 gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg cgtcttccat
    17401 cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc cgcgtttgtt
    17461 cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg cggatttacg
    17521 cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt tctgttgcag
    17581 agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa taccttcacg
    17641 ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc acgcatccgg
    17701 tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg taacggtcag
    17761 tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg actgcattac
    17821 ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga agttatcgaa
    17881 catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg catcatccag
    17941 cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca gttcgatagc
    18001 tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag ccaggaagcc
    18061 tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta acagatctaa
    18121 caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg cggccatttg
    18181 gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta cgtgcttacg
    18241 gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc aggcgattct
    18301 cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc aacgggcaga
    18361 atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa aaaagtggct
    18421 gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt aattcgcatg
    18481 gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact accgcctctt
    18541 tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga tttccttgag
    18601 atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct aatttgcagt
    18661 gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt ggaagcgcct
    18721 gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac gcttcctgca
    18781 acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag cactgcacaa
    18841 atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc gcaggtgctg
    18901 atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca cgataatcca
    18961 cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga cgccagcgtt
    19021 aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc gcaatgggtg
    19081 caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc ggtacagcga
    19141 ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca gaaagttatt
    19201 accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt cataccatcc
    19261 aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt catttccatt
    19321 gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt ggtttacggg
    19381 aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg agaaccgttt
    19441 gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac gtacgcacta
    19501 ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga cgatgatgat
    19561 tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa cggcgacaac
    19621 atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac tgataagaat
    19681 atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga tcaggatatg
    19741 gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca gctacctgct
    19801 ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg aacacacgcc
    19861 gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt taatgaagct
    19921 gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc actgacccag
    19981 cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca gcgccaggca
    20041 gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc ggcgcgccag
    20101 cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat gcagactaac
    20161 ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc aactcccgaa
    20221 cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca gcaacgtgat
    20281 aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt tcgggagaaa
    20341 gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag gctgaataac
    20401 gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag actggaaagg
    20461 taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc cggatgttcc
    20521 ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta atgctacctt
    20581 cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg tggatggtca
    20641 gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca cccagcagct
    20701 cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct ttgtcgaaaa
    20761 tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa gcagcccacg
    20821 ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca gtcagaccta
    20881 cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga tcgaagtagt
    20941 gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct ttgtgtgcca
    21001 gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg gtacgcagct
    21061 gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg cctgcgtcga
    21121 ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa cgctgcggtt
    21181 cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc gcttcaacgg
    21241 cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg gtgaagactt
    21301 taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc acgtcaatct
    21361 gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg ccacccatta
    21421 ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc cagatacgcg
    21481 gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca atgatcgcat
    21541 tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg ttagccccgg
    21601 tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata ccagccagac
    21661 caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca atatcggttt
    21721 tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg ataacttttg
    21781 ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca ccgccaaggg
    21841 tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg aaggcagcgg
    21901 agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca gtactcatca
    21961 tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc gcagcggcgt
    22021 tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat tctggcgctt
    22081 gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta accaccaccg
    22141 ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca acaaacggca
    22201 accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag aaaaaagccg
    22261 gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac agcgtgttgt
    22321 taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg tcgaaggaga
    22381 gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc ggctccatcg
    22441 cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc gcgaagtggg
    22501 tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt tcgcccgcag
    22561 tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac agctcgatga
    22621 tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg gtatccaccg
    22681 ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc gacagcgaac
    22741 tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg aagacaaccg
    22801 gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt ttacggaacg
    22861 cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc atcacccagt
    22921 tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact tcttcgattt
    22981 cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata ccaccgccag
    23041 tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc tgaacgtctt
    23101 cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta acgccaaact
    23161 cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt tcagcaaatt
    23221 cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga gccggcgctg
    23281 ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa
    23341 tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg
    23401 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc agtgactgtt
    23461 cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact tcgtcaccgc
    23521 cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc gctgccggag
    23581 tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca
    23641 taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtac
    23701 ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg atcagcgact
    23761 gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc
    23821 tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct gctgccggag
    23881 ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat
    23941 cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt
    24001 taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct tctacggtga
    24061 tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg gtgatttcaa
    24121 cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt ttacctctta
    24181 cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg cgtcagcaac
    24241 cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa ccacgacata
    24301 agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc
    24361 atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa
    24421 cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct gagcgatata
    24481 cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat cctgaccatc
    24541 acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc cgtaatcttt
    24601 cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc ccagcagctg
    24661 aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac ggataccttc
    24721 ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta
    24781 gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca taatgacagc
    24841 aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca gcgactgaat
    24901 gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac gaccggaagt
    24961 accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca gatcgccgat
    25021 acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca gattgttcgt
    25081 gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt tgatcccttc
    25141 ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg
    25201 ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac cttccatacc
    25261 gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg acttgttctt
    25321 cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt tgctctgctc
    25381 ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg tgaagttcgg
    25441 ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat aggtatgctc
    25501 ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat cagacaccgg
    25561 cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct taacctggtg
    25621 cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag
    25681 gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt agattttctt
    25741 cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt ctgcaaccag
    25801 tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac catctttcga
    25861 tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca gtttaccgct
    25921 ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga tcacgttcca
    25981 gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac cggtgaccgg
    26041 gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca gtttttcacg
    26101 ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt cgccgaggaa
    26161 cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat atttcaggaa
    26221 tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg ggaactgcca
    26281 gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt gaacttcctg
    26341 acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac gggcgtaaac
    26401 gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc
    26461 acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat
    26521 gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga tggcgttcca
    26581 gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc
    26641 ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta cgtttacacc
    26701 gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc
    26761 ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc
    26821 atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg
    26881 gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta cgtttagtta
    26941 ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc
    27001 actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc tcttcacggc
    27061 ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt
    27121 cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg
    27181 gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca
    27241 tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg
    27301 caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca
    27361 gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta ctacgcagcg
    27421 cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg
    27481 attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc cataggctgc
    27541 tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt
    27601 gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt tcgcgttccg
    27661 gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc agttgctgct
    27721 caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt tcctgtctta
    27781 agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa
    27841 agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg cacattttaa
    27901 caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga ctttacgtag
    27961 gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg attcacttac
    28021 caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca tattgagagg
    28081 ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata
    28141 aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt ctttttgtaa
    28201 caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc aaacttacac
    28261 acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga tggaaggtca
    28321 acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct
    28381 gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac agtccgcagg
    28441 gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca
    28501 gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa
    28561 atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt
    28621 agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt atccggaaat
    28681 cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa
    28741 cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta ttgcggtagt
    28801 agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc cgcaggcgag
    28861 cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg gactggtgat
    28921 gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc
    28981 agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta tctaccgcat
    29041 cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt
    29101 taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc
    29161 gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata
    29221 ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa aagcgctggc
    29281 gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc tggttacagc
    29341 attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt taatggcact
    29401 ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata tgaagttccg
    29461 tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg
    29521 taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg
    29581 aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct
    29641 gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg
    29701 tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt agcgcaatca
    29761 ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca
    29821 ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt aatacctgat
    29881 attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat
    29941 gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa
    30001 gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc gccagccagt
    30061 attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag gttcccccca
    30121 caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt
    30181 gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga
    30241 gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag
    30301 catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca
    30361 ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca
    30421 cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc
    30481 gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct
    30541 cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcgc
    30601 acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc
    30661 acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg cccgcgtaac
    30721 cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc cttcaagctc
    30781 aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga ctcccgcatg
    30841 ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca
    30901 gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat
    30961 agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat
    31021 attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt aatgtggtga
    31081 gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat
    31141 atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc
    31201 tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga cgagctgctg
    31261 gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga agatttaggc
    31321 ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa ttctcgctct
    31381 catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg ggttgaagag
    31441 gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat
    31501 gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg
    31561 ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc
    31621 cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg caaaacctta
    31681 cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt
    31741 ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc cggctcagtg
    31801 cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga agtcgaagta
    31861 gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat catgctggat
    31921 aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg
    31981 gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac
    32041 tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt
    32101 cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac
    32161 tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg
    32221 aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta
    32281 gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga
    32341 actgatggtg gttattggca tcattgccat tttaagctcc attggtattc ccgcttatca
    32401 aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac
    32461 cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa
    32521 tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt
    32581 ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga cgccaggttg
    32641 ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa gtgacagcgc
    32701 attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat
    32761 gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc tggatgccag
    32821 cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc tggacgcatt
    32881 gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg
    32941 tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc atcagcccaa
    33001 agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca
    33061 tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc
    33121 tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa aagtgctggg
    33181 aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc
    33241 aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt
    33301 attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg gaatgcagcc
    33361 gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac
    33421 tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca caccaattcc
    33481 accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg
    33541 gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca ttgtcgccga
    33601 cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct gccccactgg
    33661 caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc cttgtttgaa
    33721 gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga agttgaatcg
    33781 ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc
    33841 gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc
    33901 aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat
    33961 gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga tggttacccc
    34021 actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga
    34081 agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg aagggctggc
    34141 tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca
    34201 cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag aggcatttcc
    34261 gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg
    34321 ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag tgaaatcagc
    34381 gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca
    34441 ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact
    34501 aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc tgctggtgtt
    34561 gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga cctggcttat
    34621 cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga ggggacaaaa
    34681 actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttaca
    34741 gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca
    34801 aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa atgccggtga
    34861 gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggacct
    34921 catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct
    34981 cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt
    35041 ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg
    35101 ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat gcggttttct
    35161 tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg tgaagcccca
    35221 acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaacctgg
    35281 cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc aacgccgcca
    35341 acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg
    35401 ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg
    35461 acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt acagccaccg
    35521 tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa
    35581 agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg
    35641 ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc accagtcact
    35701 acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc
    35761 acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa tgccgggttc
    35821 aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt agaaaccatc
    35881 acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa
    35941 aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc catagaaaat
    36001 gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa
    36061 tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt
    36121 ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc
    36181 ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg
    36241 cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa
    36301 tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg ctcattttta
    36361 acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata tagttgcctt
    36421 aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg atctcggaat
    36481 taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct
    36541 acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa cattgcagcg
    36601 ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgacct
    36661 gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa cttcccccta
    36721 tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt
    36781 gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt
    36841 aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc
    36901 cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc
    36961 ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta
    37021 atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc
    37081 attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg
    37141 ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc
    37201 actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg gattggcgtg
    37261 cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc ggcggtgagc
    37321 gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg
    37381 gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac
    37441 atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc
    37501 ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc
    37561 ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg
    37621 ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc
    37681 cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg
    37741 gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc
    37801 ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc
    37861 aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc
    37921 agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct
    37981 gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat
    38041 gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg caattaccgg
    38101 ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc actcaccggg
    38161 ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga accacagagt
    38221 tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt
    38281 aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt cgcccatttc
    38341 aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgtgt
    38401 tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat
    38461 tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac tgttagcttt
    38521 tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg
    38581 ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc tgcagagtcg
    38641 tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat acgacgctgt
    38701 tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag cgtactgata
    38761 acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat
    38821 tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg cagatagtcc
    38881 atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac gcctttctcg
    38941 aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac ttcgatggac
    39001 tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt atccagccac
    39061 tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata
    39121 tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc tttgaacaca
    39181 tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag ttcgttacgc
    39241 tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta
    39301 cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc
    39361 cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact
    39421 cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa
    39481 ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg acgggattcg
    39541 tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc gtgacgtacc
    39601 tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag cgcggcaact
    39661 tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat gttggtcgcg
    39721 atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat
    39781 ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga caccagctcc
    39841 gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt acgttctttg
    39901 atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaga
    39961 tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt gtagatggag
    40021 ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt ttcatacaga
    40081 cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca
    40141 ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc ctgcatggta
    40201 cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtca
    40261 cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg
    40321 gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca
    40381 atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag
    40441 tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata
    40501 attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc
    40561 ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt
    40621 ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta gtcaaagccg
    40681 tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct
    40741 ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag cggacggttg
    40801 ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg
    40861 gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc
    40921 atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga
    40981 cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc cgggatcaga
    41041 ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt ccctttcagt
    41101 tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg
    41161 cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa caatttgatt
    41221 agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag taaaggttta
    41281 ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac
    41341 gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc
    41401 agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg
    41461 cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca ttgcgaaaga
    41521 aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg aatagttcga
    41581 attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga
    41641 aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag
    41701 cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc agaagtagcg
    41761 tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgaa
    41821 ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca gcacgaatga
    41881 agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct
    41941 tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca gatttgccag
    42001 tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg cgctttgaag
    42061 gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc
    42121 aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc ggtaaatgca
    42181 ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc
    42241 acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg
    42301 atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca
    42361 cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag
    42421 ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa
    42481 tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga
    42541 acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat accggcgtca
    42601 agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta
    42661 acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag gtgctctacg
    42721 gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca
    42781 gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc
    42841 ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt atgtaaaccg
    42901 acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc
    42961 caaattacct atccaaccga agtgtactat acattcggcg ggccagttta gcacaaagag
    43021 cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga atgctgggat
    43081 atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta ctttagcaac
    43141 cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat ccatcactgg
    43201 ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc
    43261 tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat
    43321 aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc
    43381 atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg cgccagacag
    43441 gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt ccgcacggtc
    43501 ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca ttacggtgcg
    43561 tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg cgataccttg
    43621 cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg agataccgcg
    43681 gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga
    43741 cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa agttgaaagg
    43801 cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg
    43861 tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc
    43921 ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc caacttctgg
    43981 attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac
    44041 cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat
    44101 acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt
    44161 aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc
    44221 tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt ttcgcagcca
    44281 actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc
    44341 ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg tcgaataata
    44401 cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg
    44461 cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg cgccaccggt
    44521 taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa
    44581 ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct cgatcacctc
    44641 tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg gcacttccac
    44701 gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc
    44761 ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc
    44821 ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt
    44881 accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga
    44941 ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac
    45001 cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca cttttgcctg
    45061 catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt caatcgcata
    45121 ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg atttcgcggt
    45181 atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag gcaccatacc
    45241 aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg
    45301 acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga ccacggattc
    45361 gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca cgccaatgat
    45421 attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat
    45481 ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc
    45541 tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca
    45601 gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt
    45661 aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc ccggccaaga
    45721 ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct
    45781 tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac
    45841 acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcga
    45901 tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc attccaccgc
    45961 gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg
    46021 ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat gttgacatcc
    46081 tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat atcgtcatta
    46141 cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct
    46201 tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg
    46261 aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgctg
    46321 tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac
    46381 caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt
    46441 catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt ccagcataac
    46501 gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg
    46561 caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga
    46621 gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta ttgacggtaa
    46681 aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa
    46741 tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac
    46801 tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc
    46861 cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact ctgcgcgggt
    46921 taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt tgctgcgtag
    46981 tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca agcccatcag
    47041 ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg cgataaacac
    47101 tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt cgacaggata
    47161 cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat tcagagaaac
    47221 ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca tgttgttctt
    47281 cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt tttccgatat
    47341 taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc agcatctcgg
    47401 ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca cgaattgtgc
    47461 gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga tacacttcca
    47521 gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgcg
    47581 tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc cagcctgcgc
    47641 gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca tcgaccagca
    47701 ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac
    47761 ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg tcaattccct
    47821 cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat
    47881 tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc ccctgcgggc
    47941 caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg taagttgtgg
    48001 tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca cacatcaccg
    48061 cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt aaattctcaa
    48121 agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg
    48181 gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat
    48241 gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg aaggttgggt
    48301 cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt ttgccgtgcg
    48361 ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac
    48421 ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg gcagaaatgg
    48481 cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa
    48541 tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac
    48601 tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccaa
    48661 tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg
    48721 ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag
    48781 gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc gctcggtggc
    48841 atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca
    48901 cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga taattttggc
    48961 tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa
    49021 cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta aagcaccgga
    49081 gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattcagt
    49141 cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc
    49201 tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat
    49261 tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac gaaccggacc
    49321 ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg tacgcaccgg
    49381 gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca ctttggtagc
    49441 aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat gaagtacaac
    49501 cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc cgcccatacc
    49561 gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcgttgaa
    49621 gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac cagagatacg
    49681 aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcggcagt
    49741 ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg ccagtcccgg
    49801 gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt gaccactcat
    49861 cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac
    49921 agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact gattagcggc
    49981 aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg
    50041 aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct
    50101 aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat taaaagtgct
    50161 agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa gtcagtgtgc
    50221 gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc ccaaagttcg
    50281 ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa gggatcttcc
    50341 cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag gatcagcaac
    50401 acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc
    50461 aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt
    50521 gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa gccgcgcagg
    50581 ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca aaacagcgac
    50641 agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc
    50701 cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga tccgagcagc
    50761 atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga
    50821 ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg atcgttggtt
    50881 aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcgagg
    50941 ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc
    51001 cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat actgaatcct
    51061 ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca
    51121 gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg
    51181 ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag cgcatagcct
    51241 gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc tgcgcgccgt
    51301 cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc agtgggctaa
    51361 agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct acgtgcaggc
    51421 cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta atccagcgca
    51481 caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag gtggttaacg
    51541 ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc gccaccagcg
    51601 cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc actttctcgc
    51661 ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca cccatgttga
    51721 cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag gcatcatcag
    51781 cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca cgatactgtt
    51841 gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg gtcgctgcta
    51901 ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc acgtacagtt
    51961 cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc acaccaacgt
    52021 taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag
    52081 aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg atatcgccaa
    52141 cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt gcaataccgg
    52201 gactggcgac aatcagatct gccgccatca gccattcatc attcagactg cccgtgtggc
    52261 gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc
    52321 gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg aggcccaggc
    52381 cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct tcagcgttgc
    52441 cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc
    52501 cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc
    52561 gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca cgaacacgcc
    52621 ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc ctaacgcacc
    52681 acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg tgttaaacca
    52741 caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt ccccggcgtg
    52801 tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg cccacgccac
    52861 cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt caagaccatc
    52921 ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca gaatgtagaa
    52981 cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg ttgcgggcgt
    53041 gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg acatccagaa
    53101 atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc gataatcatc
    53161 aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga cgtacggatt
    53221 ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca taatcccgcc
    53281 catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcactacctg
    53341 accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca atgagatgaa
    53401 cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc
    53461 ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa
    53521 gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat cgtaattacc
    53581 tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc agcaaaatgt
    53641 tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct taacacgcgg
    53701 tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc gctttcagcg
    53761 cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc
    53821 tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg
    53881 ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt tgccagcccc
    53941 gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca
    54001 ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc ggttggggtt
    54061 tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt gaaatcgctg
    54121 ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc
    54181 agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag
    54241 atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct
    54301 gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca ggcaatctcg
    54361 ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt taagcgcaac
    54421 agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgccg
    54481 cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga ggaccccgtc
    54541 agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc
    54601 agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg gctaaccagt
    54661 agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc aaaacgttca
    54721 cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt ggttacagca
    54781 tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag ttggctaagg
    54841 gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga tcggagtagt
    54901 ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg accagtacca
    54961 catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg ccttccatca
    55021 ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggtt
    55081 cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct tcggcaattg
    55141 cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag acgcaccaca
    55201 gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat
    55261 gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc
    55321 aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag cccagcgcca
    55381 gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc agatggcttt
    55441 caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg tcgtgatagt
    55501 tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga tcttccattg
    55561 ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca tcgtcggcgt
    55621 taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca gcttcgtagt
    55681 gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag accgacgccg
    55741 gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact tccattgcgc
    55801 aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca actgccgaac
    55861 cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg gtgcccatta
    55921 ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc gtcgtggttt
    55981 tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca tggtaaaagc
    56041 ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc ggtacgccgt
    56101 gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata atggcagcca
    56161 caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag
    56221 ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc
    56281 gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct
    56341 gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggc
    56401 tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg cgcggaaacg
    56461 gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc
    56521 ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat gtagcgaccg
    56581 tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc tttaatcgcc
    56641 gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac cacggtgcga
    56701 acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac
    56761 agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacggt
    56821 gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt
    56881 ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt
    56941 ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc cagcttggaa
    57001 acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct gtagcgtgcc
    57061 acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac cgagttttcc
    57121 cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt tgagcccggt
    57181 tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc gctcagattg
    57241 ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt gacatccacc
    57301 agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag ttcgcgataa
    57361 accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc ctggctgtca
    57421 gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc
    57481 ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc ttgactatcg
    57541 acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta acggcgagac
    57601 tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc catgtcagag
    57661 tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat gcgggcagaa
    57721 agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc accgacgctg
    57781 ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc
    57841 accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga
    57901 acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat aacttgtaac
    57961 cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca
    58021 aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc
    58081 gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga
    58141 tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg
    58201 agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc
    58261 accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt taccacggtc
    58321 accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga
    58381 tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga tcctttaact
    58441 ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca atacgcagaa
    58501 ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt aacttgccta
    58561 gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg
    58621 ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca
    58681 gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt
    58741 ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg
    58801 tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc acgacttccg
    58861 ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gcgcgggcaa
    58921 tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata tcggcttctt
    58981 ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca
    59041 gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc ggtgaagaga
    59101 cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg gcaacgtatt
    59161 cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg
    59221 caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct
    59281 gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat
    59341 caggacggat attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt
    59401 tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc ggtagccaac
    59461 tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca
    59521 aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt
    59581 actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac
    59641 agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga taatttttgc
    59701 tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg
    59761 gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg ggtaggcact
    59821 gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca
    59881 ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa
    59941 aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat
    60001 atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg aatagctgta
    60061 agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc cacagcaaat
    60121 ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt
    60181 ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg
    60241 tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac gcgctgtcga
    60301 tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg tttaaaacca
    60361 tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAJTRM010000013        58332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRM010000013 DAJTRM010000000
VERSION     DAJTRM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.013"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1101)
                     /locus_tag="OO322_000866"
     CDS             complement(<1..1101)
                     /locus_tag="OO322_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5905844.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO322_000867"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO322_000867"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5905845.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO322_000868"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO322_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5905846.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO322_000869"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO322_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5905847.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO322_000870"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO322_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5905848.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO322_000871"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO322_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5905849.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="OO322_000872"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="OO322_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5905850.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO322_000873"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO322_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5905851.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO322_000874"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO322_000874"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5905852.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO322_000875"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO322_000875"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5905853.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO322_000876"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO322_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5905854.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO322_000877"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO322_000877"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5905855.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="OO322_000878"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="OO322_000878"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5905856.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="OO322_000879"
     CDS             11851..12015
                     /locus_tag="OO322_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905857.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="OO322_000880"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="OO322_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5905858.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="OO322_000881"
     CDS             13524..13769
                     /locus_tag="OO322_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5905859.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="OO322_000882"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="OO322_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5905860.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="OO322_000883"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="OO322_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5905861.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="OO322_000884"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="OO322_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5905862.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="OO322_000885"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="OO322_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5905863.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="OO322_000886"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="OO322_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5905864.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="OO322_000887"
     CDS             19432..19785
                     /locus_tag="OO322_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5905865.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="OO322_000888"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="OO322_000888"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5905866.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="OO322_000889"
     CDS             21085..22212
                     /locus_tag="OO322_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5905867.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="OO322_000890"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="OO322_000890"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5905868.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="OO322_000891"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="OO322_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="OO322_000892"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="OO322_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26571..26744
                     /locus_tag="OO322_000893"
     CDS             26571..26744
                     /locus_tag="OO322_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905869.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="OO322_000894"
     CDS             26834..27583
                     /locus_tag="OO322_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5905870.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="OO322_000895"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="OO322_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5905871.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO322_000896"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO322_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5905872.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO322_000897"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO322_000897"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5905873.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO322_000898"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO322_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5905874.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO322_000899"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO322_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5905875.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO322_000900"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO322_000900"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5905876.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="OO322_000901"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="OO322_000901"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5905877.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO322_000902"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO322_000902"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5905878.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /locus_tag="OO322_000903"
     CDS             complement(35512..35607)
                     /locus_tag="OO322_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905879.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /locus_tag="OO322_000904"
     CDS             complement(35721..35888)
                     /locus_tag="OO322_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905880.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36055)
                     /locus_tag="OO322_000905"
     CDS             complement(35891..36055)
                     /locus_tag="OO322_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5905881.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO322_000906"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO322_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5905882.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO322_000907"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO322_000907"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5905883.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="OO322_000908"
     CDS             40511..40762
                     /locus_tag="OO322_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5905884.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO322_000909"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO322_000909"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5905885.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="OO322_000910"
     CDS             42067..42825
                     /locus_tag="OO322_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5905886.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="OO322_000911"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="OO322_000911"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5905887.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO322_000912"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO322_000912"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5905888.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="OO322_000913"
     CDS             complement(44425..45045)
                     /locus_tag="OO322_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5905889.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO322_000914"
     CDS             complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO322_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5905890.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="OO322_000915"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="OO322_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5905891.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="OO322_000916"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="OO322_000916"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5905892.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="OO322_000917"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="OO322_000917"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5905893.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="OO322_000918"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="OO322_000918"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5905894.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="OO322_000919"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="OO322_000919"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5905895.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="OO322_000920"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="OO322_000920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5905896.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53107..53286
                     /locus_tag="OO322_000921"
     CDS             53107..53286
                     /locus_tag="OO322_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5905897.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53372..53872
                     /locus_tag="OO322_000922"
     CDS             53372..53872
                     /locus_tag="OO322_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5905898.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53918..54424
                     /locus_tag="OO322_000923"
     CDS             53918..54424
                     /locus_tag="OO322_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5905899.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="OO322_000924"
     CDS             complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="OO322_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5905900.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIAIVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55362..55451
                     /locus_tag="OO322_000925"
                     /pseudo
     CDS             55362..55451
                     /locus_tag="OO322_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55479..56222
                     /locus_tag="OO322_000926"
     CDS             55479..56222
                     /locus_tag="OO322_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5905901.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56252..56791
                     /locus_tag="OO322_000927"
     CDS             56252..56791
                     /locus_tag="OO322_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5905902.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56896..57294
                     /gene="yciA"
                     /locus_tag="OO322_000928"
     CDS             56896..57294
                     /gene="yciA"
                     /locus_tag="OO322_000928"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5905903.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="OO322_000929"
     CDS             complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="OO322_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5905904.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
BASE COUNT        14006 a        15089 c        14572 g        14665 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttt gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatacca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac tgcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaactcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttc cctctatctt ctcgttgcgc ttaatttgac taattctcat tagtgactaa
    53041 ttttaatgag tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga
    53101 aataacatgg ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca
    53161 tccgacgcag gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa
    53221 cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa
    53281 tcctgattca ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt
    53341 atggctcgca gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt
    53401 tattcaactg ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa
    53461 actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga
    53521 aactcatgga cagattgaac gaattgatca agttgtggaa tcagaatcaa atctgaaaat
    53581 taagcgcatg aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga
    53641 aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga
    53701 gcattatgag attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg
    53761 taaagcagcg aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact
    53821 gactgatctg gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa
    53881 ttttaacttt tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca
    53941 tgactggtta cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat
    54001 ggccagccgt atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga
    54061 aaccaaaaac cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc
    54121 agtcattaaa gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt
    54181 cccttctgat gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat
    54241 cacctgttac acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc
    54301 gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat
    54361 tccgcaaaca actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa
    54421 ataataccct ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg
    54481 gaattaaaaa cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc
    54541 gtgttgctgt gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac
    54601 tgacatgtta accaaccagt cacggttaat cagataatca acccccacct gcccggcagc
    54661 tccccaggaa tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa
    54721 tccattatca aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct
    54781 gctggcatca ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacgattgc
    54841 aatatcgccg gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc
    54901 cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt
    54961 cacgctgaat ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt
    55021 tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag
    55081 aagagttgtt actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg
    55141 gttataaaaa cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac
    55201 attacatatg ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat
    55261 cctggttaat gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga
    55321 tcgccacttg ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag
    55381 atatgctttt ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc
    55441 ccgctaatta acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg
    55501 tataccgtga caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt
    55561 tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc
    55621 ttgcgcagct caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc
    55681 agaatatgtc accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt
    55741 cagaattaat cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt
    55801 ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca
    55861 agttatttat tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg
    55921 tcgttccggg aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg
    55981 acaaaatggg catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc
    56041 gtctggtggc acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg
    56101 cctcttcttt tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca
    56161 acctgatttc tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat
    56221 aaccttaagc atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct
    56281 ggttgtcttt ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat
    56341 cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat
    56401 ggccctgatc acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa
    56461 tgatgagttt attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt
    56521 agtcagccaa tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac
    56581 gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg
    56641 cggtctggca aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa
    56701 agtctttggc ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta
    56761 ccgccacatg ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc
    56821 tgtatctcct ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt
    56881 taagtcggtt ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt
    56941 actttagcca tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg
    57001 tcacaaatgg atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg
    57061 actgtgcggg ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc
    57121 tgctatgcac gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg
    57181 gtgaaaaaag tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt
    57241 aagtatgtag cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca
    57301 aaagcctccg gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat
    57361 tttaaacagg atattcacca caatcccact gcctggctta cccggctcat aacgccatct
    57421 gcgcatcgca tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg
    57481 tacgttatcc acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat
    57541 gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc
    57601 tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg
    57661 cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg
    57721 ttttggctgc tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg
    57781 cttttcaatg accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc
    57841 tggctctacc accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg
    57901 cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac
    57961 cgaggtatag agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg
    58021 ccaggggaag cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa
    58081 actaggcctc gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga
    58141 tcagtgaaaa tgtcattttt ggcgtaatta cccttacttt atgcatgacc ttaacaaaga
    58201 catcatcttt ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga
    58261 acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga
    58321 aaaacgcctt tc
//
LOCUS       DAJTRM010000014        58035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRM010000014 DAJTRM010000000
VERSION     DAJTRM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.014"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(26..280)
                     /locus_tag="OO322_000930"
     CDS             complement(26..280)
                     /locus_tag="OO322_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5905905.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO322_000931"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO322_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5905906.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO322_000932"
     CDS             1230..2696
                     /locus_tag="OO322_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5905907.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO322_000933"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO322_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5905908.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO322_000934"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO322_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5905909.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO322_000935"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO322_000935"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5905910.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO322_000936"
     CDS             5711..7267
                     /locus_tag="OO322_000936"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5905911.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO322_000937"
     CDS             7349..9163
                     /locus_tag="OO322_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5905912.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO322_000938"
     CDS             9164..10258
                     /locus_tag="OO322_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5905913.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO322_000939"
     CDS             10258..11283
                     /locus_tag="OO322_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5905914.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO322_000940"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO322_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5905915.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO322_000941"
     CDS             complement(12878..13222)
                     /locus_tag="OO322_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5905916.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO322_000942"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO322_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5905917.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO322_000943"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO322_000943"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5905918.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO322_000944"
     CDS             15618..17378
                     /locus_tag="OO322_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5905919.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO322_000945"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO322_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5905920.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO322_000946"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO322_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5905921.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO322_000947"
     CDS             19115..19342
                     /locus_tag="OO322_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5905922.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO322_000948"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO322_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5905923.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO322_000949"
     tRNA            21197..21273
                     /locus_tag="OO322_000949"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO322_000950"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO322_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5905924.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO322_000951"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO322_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5905925.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO322_000952"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO322_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5905926.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO322_000953"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO322_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5905927.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO322_000954"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO322_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5905928.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO322_000955"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO322_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5905929.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO322_000956"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO322_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5905930.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO322_000957"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO322_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5905931.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO322_000958"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO322_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5905932.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO322_000959"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO322_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5905933.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO322_000960"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO322_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5905934.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO322_000961"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO322_000961"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5905935.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO322_000962"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO322_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5905936.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO322_000963"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO322_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5905937.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO322_000964"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO322_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5905938.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO322_000965"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO322_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5905939.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO322_000966"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO322_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5905940.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO322_000967"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO322_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5905941.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38302..39333)
                     /locus_tag="OO322_000968"
     CDS             complement(38302..39333)
                     /locus_tag="OO322_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5905942.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39567..39926)
                     /locus_tag="OO322_000969"
                     /pseudo
     CDS             complement(39567..39926)
                     /locus_tag="OO322_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO322_000970"
     CDS             complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO322_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5905943.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO322_000971"
     CDS             complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO322_000971"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5905944.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO322_000972"
     CDS             complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO322_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5905945.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO322_000973"
     CDS             complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO322_000973"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5905946.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO322_000974"
     CDS             complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO322_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5905947.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46677..49349
                     /gene="rcsD"
                     /locus_tag="OO322_000975"
     CDS             46677..49349
                     /gene="rcsD"
                     /locus_tag="OO322_000975"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5905948.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49366..50016
                     /gene="rcsB"
                     /locus_tag="OO322_000976"
     CDS             49366..50016
                     /gene="rcsB"
                     /locus_tag="OO322_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5905949.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO322_000977"
     CDS             complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO322_000977"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5905950.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53340..54116)
                     /locus_tag="OO322_000978"
     CDS             complement(53340..54116)
                     /locus_tag="OO322_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5905951.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54121..55770)
                     /locus_tag="OO322_000979"
     CDS             complement(54121..55770)
                     /locus_tag="OO322_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5905952.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55771..56330)
                     /locus_tag="OO322_000980"
                     /pseudo
     CDS             complement(55771..56330)
                     /locus_tag="OO322_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56489..>58035)
                     /locus_tag="OO322_000981"
     CDS             complement(56489..>58035)
                     /locus_tag="OO322_000981"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5905953.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14197 a        15655 c        14258 g        13925 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caacttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcagcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attattttta tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccatg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccgacagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcacccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcga ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc actgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttga ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcggcgcac tgatgaccat
    13861 ccacgcagcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgacaacc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctaaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccagtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc agcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctagtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgttttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg cccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taataaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgaaggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgttaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaagt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc tagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccatgatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 ggtttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tagcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg actgttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcaaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg aagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc attacaacgc aatatccgcc acttcttttt gaacgggttg
    38341 cggtttcaac tgcggtttcg gcgtactatc tgctgcttgc ggtttgtcgt atttcagccc
    38401 cagcacttcg ctggtgtact gcaactcgcg ttctgttgcc gctacatcac cgttcagctt
    38461 gcgtccataa gacggaacga tcgctttcaa cgtagcctgc cattgcgggc tggaaacgcg
    38521 atcgccaaat actttttcca gtagattcaa cataatcggc gcggcggttg acgcccctgg
    38581 cgatgccccc agcagcgccg caatggtccc ttgctggtca ctgaccactt cggtacccag
    38641 acgcagtacg ccacctttct cggcatcacg cttgataatc tgcacacgct gccccgcttg
    38701 ccacaaacgc cagtcctctt ttttcgcttg cggatagtac tctttcaacg cttcaaaacg
    38761 atcctcttca ctcaacatca cctgactcac cagatatttc accagatcga aattatccag
    38821 cccgacgtgc atcatcggca tcacgttaga ggtggtggtg gaactcatca gatcccacaa
    38881 tgaaccgttt ttgaggaatt tggttgagaa ggtggcaaat ggcccaaaca gcactacgcg
    38941 tttaccgtcc agaacacggg tatcgatatg cggaaccgac atcggtggtg cgccaacgga
    39001 tgctttaccg taaactttcg ccagatggcg attaaccacg tctggatttt ccgaaacaag
    39061 gaactgtccg cccaccggga aaccggcgta gtctttcgct tccggaatcc ccgattcctg
    39121 taacagcttc agcgccgcac cgcccgcgcc gataaagaca aatttggcgc ggatgttctg
    39181 tgcagtgccg tttttcagat cggcaacggt aacggtccag gtgttatcgt cattacgctt
    39241 tagggcgcgg acttcgctgc tgagttgcag cgagaagtta gatttcttct gtaaggaagc
    39301 aattaactgg cgggtgatct caccatagtt cacatcagtg ccaatttctg ttgcggatcg
    39361 cgcccttcca tcactaacgg tgcccactct ttgatctgcg cgtgatcttc agagtaacgc
    39421 ataccgcgaa acagcgagct ttgttgcaac gcggcgtaac gggcgcgcag gaaattaacg
    39481 ttatcctcgc cccagacaaa gctcatatgc ggaacggtat tgataaatga acgcggagta
    39541 cgcagcacgc cgcgctcgac ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg
    39601 ttaatggcga ctgctttttc aatactgatg ctgccatcgg cgttttgcgg ggtgtagttc
    39661 agttccatca gtgcagaatg cccggttccg gcgttgttct agccgttcga actctcctgc
    39721 gcgcccccct ccaggcgctc caccatggtc atcgaccatt caggctccag ctcgcgtcaa
    39781 taggtcccca acgtggcgct cataatgccg ccgccaatca acagtacatc agtttcctgc
    39841 tcctcggacg ctttcgcttt tgccgccatc gaaacggcat taagccccac ggccatcgag
    39901 aagagcatgg cagtcacttt tttcatcttg ttaatgcctt acttttagtc gctttattgc
    39961 aggtgagatt tgcgcggcat caacggtaac acttaagtaa caacatttaa ataatgttta
    40021 taaattatat tctaatttta gaaacattat aatttcgttg attaattata gggttattag
    40081 aaatgaaggg atttttagca tattgacctg acggcagcag tccgccaggt cagcgtcgta
    40141 atattatgcc gtccgggcaa cggcatcacg cgaagcggca tcgcgctctt cgcccgtcag
    40201 ctcgctaagt tgcccattgc gcatttccag caggcggtcg gcgtggataa agtaatgatc
    40261 atcatgactg atagcgaaaa tagttttacc catctcctgc atcagcggca gcaacacctg
    40321 ataaaactca cgacggaagt gtggatcctg atccaccgcc cattcatcca gcaggataat
    40381 atcgcgttct tctgccagcg ccagcaacag cgccacgcgt tttttctgcc cttttgataa
    40441 cttcaggtta acaatacgcc cgttgcttaa ctcaagctta tgagccattt tcagctgcgc
    40501 cagccacttc tcaaccagtt gcgggttagc gggttgcccc tccggcccca gcagttgatc
    40561 aaacagccag acatcggtaa acactgccga aaacagtttg cgataatctt ccggttgttc
    40621 gccgctgaca ggtttgccat ccagcaagat ttcgccgctt tgtggctgat acaagcccgt
    40681 caacaacatc gccagcgtcg attttccgct accgttaccg ccaatcagaa acagcagctc
    40741 gccacgtttg atggtgagat taatcggacc aacggaaaac gcgttatcct gataagcaaa
    40801 cgtcacgtta cgcagctcca gcgtttgcca gttgggaaac gcctgcgggc gcggaaactc
    40861 agctttgaaa ggcgcgagcg cgaatttgtt cagcttgtta aacgccacct gcgccgtcag
    40921 cagcgtcggc aatgcgccaa ccgccgaaag cagcggcgta cgcaggaata acagcgtcaa
    40981 cgaataggtc gcagcaacgt tggtatcagc ccaaccgaga ctgttcgcca tccagaacac
    41041 caggccgata gcgcccagca tcatgatgtt tgaccagttc acggcactaa gatggaaggt
    41101 gtctgcgcga ataatatgat ggcgatactc ttgcgcatcg gggatgtaga ggttgttaaa
    41161 cacatactcg gcgcgttccc ggttcagagt cagctctttg cgcccttcaa gtacagtttg
    41221 aaaatccgtg tacagcttgt cttcggtttc acgtagggtc gccatatgtt tgtacacccg
    41281 cgccaccagc acaaaaccgc cccagatggt gatcgccatc cagatagccg ttaccagcaa
    41341 cattttgccc gacagcatcc acagatacgc cgatgaaccg atagtgagaa tgatcccctg
    41401 ccccagttcc ggcagacgca caaaagcaat ggtgatattg cgcacatcgc tggttaatcc
    41461 cgccagcaac gaggcgctac cgagttgttc aatgcgctcg acgtgggtat ccagaatccg
    41521 cttgataaat tcactacgca gtcggtaaac gaagtgatgc cccaaagtgg tgagcgccag
    41581 ttgcgatccg agagtgactg ccatcaacag cagcaataat cccagaaact ccggtaacac
    41641 cagcagactg gtatccgccg tttcgataag gcgctgattg ataaaagcaa ttaagccaat
    41701 gcctaatgcc gcactggcga ggcttagcgc catcacactg ataaatggcc agcgatactg
    41761 ccgccagaca agtacaagaa gttccatgca gaaaacccgg acaatgaatt acagcccgca
    41821 gtttaaacat cttcgcgcgc acagcaataa taattcttat ttttattctt ttttacctgc
    41881 ctgacggaat gtcaggttgt agcggcagtc ggtggtgagt ggatgaaacc ccgctttcaa
    41941 cggttgaata ccgtgataaa acagccgcga ttcaccgccc cataccacca catcgccatg
    42001 ttccaacaac aaacgtttga gcggatcatt acgtttcagg ccaccaaatt gaaaaatcgc
    42061 gggtaagccc agagaaacag aaacaattgg cgcgcgcaga tccggttcgt ctttatcctg
    42121 atgcagcgac agtttcgcgc caggagcgta gcggttgata agacaagcat ctggctggaa
    42181 atctggatag cccgccgccg tagccgcacg ttgacataaa tcatgaaagc tttgtggcat
    42241 agcgggccac ggtttatttg tttgcggatc aatgggcgaa tagagataac cttgccgatg
    42301 ggtcgtccag cccagatgcc cacagttggt catcgccacc gacatggtat atccgcctgg
    42361 agcgaccatc tggcgaaacg gcgactggct ggcaacgtca ttaatatcgc ggattagttg
    42421 ctccgcagca ttaaaagcaa aacgccgtaa aattaccgca ccagccgcca gtggctcttg
    42481 ccacggttca gcatcggcaa acagatccaa cattacctct cctcattttc agcttcgcgg
    42541 cgcagcagtt gcgctttacg cgacacgccc cagcggtaac cggaaagtgt gccatcacca
    42601 cggaccaccc gatgacaggg tattacgata gccagcttgt tggcggcaca ggcgctggca
    42661 acggcccgta ccgctttcgg tttgccgatg gcgttagcca gttgctgata actgacggtt
    42721 tcaccacaag gtatcgtgcg cagtgcctgc cagacttgtt gctgaaaagc agtgccgcga
    42781 atatccagcg gtaacgtcag cggcgtatcg cgttgattga ggctggcgat cacttcacgc
    42841 acatgttgct gaaacgtcag atcggcaggc gcgttgtcgg cagcgggaaa catctgctgc
    42901 aactcgctga ttaatgtcgc gtcatcatcg cccagcaata tcgcgcaaat cccccgctcg
    42961 ctttctgcca ccaggcaacg acccagctca caatcagcca gcgcgtaacg caccgccaga
    43021 ttttcgccac cgtgacggaa ttgtttagcc gtcatgccca gcgtttcatc ggctttgcgg
    43081 tagtaactgc tgctgtcggg gaatccggcg ttaagaatag acgtcgtcac gctctcccct
    43141 ttcgccagcg attcgcgcaa acggcgagcg cgccaggcct gttgccaggc tttaggcgtc
    43201 attccggtag tagctttaaa caaccgatgt agatgaaatg gactcatcgc cacctggtcg
    43261 gctaaggctt ccagcgttac aggcgtttcc tgttccagca gtcgacacgc gtgggtgatt
    43321 ttatccaacc gatgttgccg gggattggct ttgtctggct gacaacgttt gcaggggcga
    43381 aatccggcgg cgagtgcctc gctggcattt gcgtagaagg agacattttc ccgcaaggca
    43441 tgtctggcgc ggcaagaagg acggcaaaag atacccgtgg tgcgcacggc gaaaacgaat
    43501 tcgccgtcgg cattcgggtc gcgggctaag acagattgcc agcgttgatc gtcagttaag
    43561 catgtggcgt ttttcataat cagctccctg gttaaggata gcctttagtc tgcccggtca
    43621 ccatcacgca aaaaccaaca atcttgcgct ttaatttttt tcgctgacaa ggaagctttt
    43681 aaactgtggt gacatccagg ttttaaagct atcgccttct ttggtgatca tataaaccgc
    43741 cagcccttcc cggcgaacaa cctctttggc tttctccggc ccgagtacca tcaagccagt
    43801 atcccaggca tcggcttcca gcgccgtcgg agcaatcacc gtcacggata ccagattgtg
    43861 ttcgatggga cgcccggttt gcggatcgat aacatgggaa agacgtttgc cgtccagttc
    43921 gtaatagtta cggtagctgc cagaggtgct aatcccatga ccgttgatat ccaccacagc
    43981 ctgaaccgcg ttttctttat cggttggttt ttgaatcgct acccgccacg gctggccttc
    44041 accgttcata ccacggctgt tcagcgcgcc gcccaccgac accagatagc gggaaatccc
    44101 ttcctgctcc atcaagcgtg ccaggtgatc cgccgcataa ccttcaccga cggtggagag
    44161 atcgacatat aaatccggca ggtctttttg cagatattgc tgatgcgact gattgatgac
    44221 cgtcaggtgc tgtaagccgg ttttggcttt catcgcatcg atctgttcct ggctcggaat
    44281 ttgaaccggc tgttgttccg ggccaaagcc ccacagattc accagcggcc cgacggtaat
    44341 atccatcgca ccatcggtcc ttgccccaat gcgcagcgag gtggtgacga tatcggccat
    44401 cgcttcactt accggccacg gcgacaaact ttgcgagtcg ttaaagcgca tcagcgcgga
    44461 atctttttta taggtcgaaa gcagctgatc gtcggcgtcc agctgggtct gaatcttttc
    44521 tttaagttcg gcactgcgtt tggcatcaat gcccgggatg ctggcacgcc agaaggtacc
    44581 catggttttg ccttcaagaa cggtaacttc agtagcgtgg gttttggcgg gttgtggttt
    44641 ttgatcgcaa ccaacaaaga agagcgcggc aaccagcagt gccacgcggg taaagcttat
    44701 ttccatacgt gattatcctc atgcgaacgg tcgcaagagt acaccaaaaa actgtgtttg
    44761 tacgctgaaa acaatgaaaa aagggcccgc aggccctttg ttcgatatcc atcgagatta
    44821 gaactggtaa accagaccca gagctacgat gttatcagtg ttgatgccag cgtcacgagt
    44881 gaactggttg tcgtccagca ggttgatttt gtagtcaacg taggtggaca tgtttttgtt
    44941 gaagtagtag gtcgcaccaa catcaacata tttcaggata tcttcgtcgt cgtagttacg
    45001 accattgatg acacccaggt ttttaccttt agactgcagg taagccaggg acggacgcag
    45061 accgaagtcg aactggtact gagcaacagc ttcgaagttc tgtgctttgt tcgcccaacc
    45121 caagttgcct acgcgagttg cgttgtaggt ctgggtgtac tgagcagcta ggtagatgtt
    45181 gttcgcgtcg tatttcagac caccggtgta ggtttcagcg cggtcgccat taccgatgta
    45241 acgctcatca taacggttca gaccgaagtt ctggtcatca gtacgtttgg agctggaaac
    45301 tgcagcaccg ataccgaagc cttcgtaatc ataagtaata gaaccgccaa cgccgtcacc
    45361 gttctgacgc agagcttcac gaccgttgtt ggtcatgcct tcaccttccg ggctaccgtt
    45421 tttgccctga tactgaacag cgaagttcag accgtcaacc agaccaaaga agtcggtgct
    45481 acggtaggtt gcgaagccgt taccacgctg ctgcatgaag ttgtcagaac cgtaagtgtc
    45541 accaccgaat tctggcagta cgtcggtcca ggaagttacg tcgtaaacta cgccgtagtt
    45601 acgaccgtag tcgaaagaac ctacatcctg gaatttcaga cctgcgaatg ccacacgggt
    45661 ccaggagttg ttttcgtttt cagcgctgtt gccctggatc tgatattccc actggccgta
    45721 accggtcagc tggtcagtaa cctgagtttc acctttgaag ccaagacgca tgtaggtctg
    45781 gtcgccatct acagacttgt cgtcagagaa atagtgcagg ccgtctactt taccgtacag
    45841 atctaatttg ttgccgtctt tgttgtaaac ttcagcagcg tttgctgcgc ctgctaccag
    45901 cagagctggg accaggaggg acagtacttt aactttcatg ttattaaccc tctgttatat
    45961 gcctttattt gcttttttat gccactgcat actgattaac cctcattaat cagtcggcaa
    46021 gtccattctc cccaaaaatg cagaataatc caacacgaat atgatactaa aacttttaag
    46081 atgtttcatt tatcgctata gatgtttcaa aatgtaaata caagggaact ttttaagatt
    46141 attgcggaat ggcgaaataa gcacctaaca tcaagcaata ataattcaag gttaaaatca
    46201 ataacttatt cttaagtatt tgacagcact gaatgtcaaa acaaaacctt cactcgcaac
    46261 tagaataact cccgctatca tcattaactt tatttattac cgtcattcat ttctgaatgt
    46321 ctgtttaccc ctatttcaac cggatgcctc gcattcggtt tttttaccct tctttacaca
    46381 cttttcatta ttctgtgcta ccacagaaaa actataacgc ttgttaacta tttcacaaat
    46441 aattaacatc agcataattt ccagcaatct ttgtttattt gcaattaatt tcgttgggct
    46501 ttttgtaggt tatttgtaca gcaaaatggc gcttgtacat ctatttcccc ccatacagga
    46561 tgataaatat cacgggagaa tagagaatca tcaatcaggt aagagtctgg aatttcacac
    46621 tgtacccttt atactgccct atcacttcgc gaagttttaa caggtcataa acacgaatgc
    46681 gtcagaaaga gacaacggcc acgacccgct tttcactcct accggggagc attacccgct
    46741 tctttttact gttgatcatt gtgttactgg tgacgatggg ggtaatggta caaagtgccg
    46801 ttaacgcttg gctgaaagat aaaagttacc agattgtcga cattacccac gcaatccaaa
    46861 agcgcgtcga tacctggcgt tacgtgacca ggcagatcta cgacaacatt gccgcgacga
    46921 cctccccctc ctccggcgaa ggtttacaag agacgcgcct gaaacaggat gtctactatc
    46981 tggagaaacc acgccgcaaa acggaagcgt taatctttgg ctctcacgac aactcaacgc
    47041 ttgagatgac tcagcggatg tccacttatc tggatacatt gtggggtgca gaaaatgtac
    47101 cgtggtcgat gtattacctg aatggtcagg ataacagtct ggtgctgatc tcaaccctac
    47161 cccttaaaga tctcacctcc ggatttaaag aatcgaccgt cagtgacatt gttgattcac
    47221 gtcgtgcaga aatgttacaa caggccaacg ccctcgatga acgcgaaagt ttttctaaca
    47281 tgcgccgcct agcctggcag aacggtcatt actttacctt gcgtaccacg ttcaaccagc
    47341 cgggacatct ggcaacggtc gtggcttttg atctgccgat taatgatttg atcccaccgg
    47401 gtatgccgct ggacagtttc cgccttgagc cagacgcgac ggcaacggga aacaatgata
    47461 atgagaaaga agggacggat agcgtcagta tccactttaa cagtacgaag attgaaatct
    47521 cctcggcact caactctacc gatatgcgcc tggtctggca ggttccttat ggcaccttat
    47581 tgctggatac gttgcaaaac attctgctgc cactgctgct gaacatcggt ttgctggcgc
    47641 tggcgttatt tggctatacc acattccgcc atttctccag ccgcagtaca gaaagcgtcc
    47701 ccagcacggc ggtcaataac gaattgcgca ttttacgggc aatcaatgaa gagatagtct
    47761 cactgttgcc gctcggcctg ctggttcacg atcaggaatc gaaccgcact gtcataagta
    47821 acaaaattgc cgatcatttg ctgccgcatc tgaatctgca aaacatcacc accatggcgg
    47881 aacagcatca ggggattatt caggcgacga tcaataacga gctgtatgag atccgcatgt
    47941 tccgcagcca ggtcgcgccg cgcacacaaa ttttcattat tcgcgatcag gatcgcgaag
    48001 tgctggtaaa caagaaactc aagcaggcgc agcgtctgta tgagaaaaac cagcaggggc
    48061 ggatgacctt tatgaaaaac attggcgatg cgctgaaaga acccgcacag tccctggcgg
    48121 agagtgcggc taaactcaac gccccggaaa gcaaacaact ggcgaatcag gcagatgtgc
    48181 tggtgcggct ggtcgaagaa atacagttag cgaacatgct tgcggatgat agctggaaaa
    48241 gtgagacggt gctgttctcc gtgcaggatt taattgatga agttgtgcct tcagtgttgc
    48301 ctgccatcaa gcgtaaaggt ctgcaactgc tgattaacaa tcatctgaaa gcacacgata
    48361 tgcgccgcgg cgatcgcgat gccttacgac gtattttgct gctactgatg caatatgccg
    48421 tgacctcaac gcaattggga aaaatcaccc ttgaggttga tcaggatgag tcctctgaag
    48481 accgcctgac attccgcatt ctggacacgg gagaaggcgt aagtattcat gaaatggata
    48541 atttgcactt cccgtttatc aaccagaccc aaaacgatcg ttatggaaag gcggacccgc
    48601 tggcattctg gctgagcgat caactggcac gtaaactggg cggtcattta aacatcaaaa
    48661 cgcgggatgg gcttggtaca cgctactctg tgcatatcaa aatgctcgcc gctgacccgg
    48721 aagttgaaga ggaagaagag cgtttactgg atgatgtctg cgtaatggtg gatgttactt
    48781 cggcagaaat tcggaatatt gtcactcgcc tgttagaaaa ttggggtgca acctgtatca
    48841 cacccgatga aagattaatt agtcaagatt atgatatctt tttaacggat aatccgtcta
    48901 atcttactgc ctctggcttg cttttaagcg atgatgagtc tggcgtacga gaaattgggc
    48961 ctggtcaatt gtgcgtcaac ttcaatatga gcaacgctat gcaggaagcg gtcttacaat
    49021 taattgaagt gcaactggca caggaagagg tgacagaatc gcctctgggc ggagatgaaa
    49081 atgcgcaact ccatgccagc ggctattatg cgctctttgt agacacagta ccggatgatg
    49141 ttaagaggct gtatactgaa gcagcaacca gtgactttgc tgcgttagcc caaacggctc
    49201 atcgtcttaa aggcgtattt gccatgctaa atctggtacc cggcaagcag ttatgtgaaa
    49261 cgctggaaca tctgattcgt gagaaggatg ttccaggaat agaaaaatac atcagcgaca
    49321 ttgacagtta tgtcaagagc ttgctgtagc aaggtagcct attacatgaa caatatgaac
    49381 gtaattattg ccgatgacca tccgatagtc ttgttcggta ttcgcaaatc acttgagcaa
    49441 attgagtggg tgaatgttgt cggcgaattt gaagactcta cagcactgat caacaacctg
    49501 ccgaaactgg atgcgcatgt gttgattacc gatctctcca tgcctggcga taagtacggc
    49561 gatggcatta ccttaatcaa gtacatcaag cgccatttcc caagcctgtc gatcattgtt
    49621 ctgactatga acaacaaccc ggcgattctt agtgcggtat tggatctgga tatcgaaggg
    49681 atcgtgctga aacaaggtgc accgaccgat ctgccgaaag ctctcgccgc gctgcagaaa
    49741 gggaagaaat ttaccccgga aagcgtttct cgcctgttgg aaaaaatcag tgctggtggt
    49801 tacggtgaca agcgtctctc gccaaaagag agtgaagttc tgcgcctgtt tgcggaaggc
    49861 ttcctagtga ccgagatcgc taaaaagctg aaccgcagta ttaaaaccat cagtagccag
    49921 aagaaatctg cgatgatgaa gctgggcgtc gagaacgata tcgccctgct gaattatctc
    49981 tcttctgtga ccttaagtcc ggcagataaa gactaatcat ctgtaggcca gataagacgc
    50041 gttagtgtct tatctggcat ttgcaccgat tgccggatgc ggcgtaaacg ccttatccgg
    50101 cctacgattc ccattatttc aacagattac attaaagtag gccagataag acgcgtcagt
    50161 gtcttatctg gcatttgcac tgaatgccgg atgcggcgta aacgccttat ccgtcctacg
    50221 aatcccgcga tttcctgacc ctctcggcat ataccgtcag cgtctgtttt atcacatcca
    50281 gcgttaccgg cttcgagagg cagctgtcca taccggactc cagacaccgc tgcttctctt
    50341 cagccaacgc attagcagtt actccgatta ccggcaacgt cagtcccaac tgacgaatgc
    50401 gttgcgtcaa gcggtaacca tccatatttg gcatgttgac gtcgctaagc acgatatcaa
    50461 tatgattctt actaagtaca ttaagcgcat cgacgccatc attcgcggtt ttacattgat
    50521 agcccaacga tcccaactga tctgccagca gacgtcggtt aatcggatga tcatccacga
    50581 ccagaatcat catatcgtcg ttatcgctga ccgctttgtc cgtcgacggc agagcgttag
    50641 caggatcgtc gctctccatc tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat
    50701 gcggagcagc cacactgtgt acccactccc ctggcgcttt ctccagcgga ataccaatat
    50761 ggcgacgaca gaaggtcact accgctctgc cctgccattt tttactcact acctcgtcag
    50821 tgattaacac atcttcggga gtcggttcct gcccttcgta tgttgtaacg acgatgccgc
    50881 tgcgctgcaa actggtttcc aggaactgac agagcgacgc attgcggacc gccagccagc
    50941 agcgtttacc actcaaccct tccacgcctt ttttctgcgg gtactgagcg ccgtacaacg
    51001 gaatacgcac ggtaaactgg ctgcccattc ccggttctga atctaccgag atatcgccgt
    51061 ccatcatgct gatcagtttt tcacaaatcg ccagacccag accggtcccc tggaagtttc
    51121 gctgtacgcc cgttccaacc tggaagaagg gatcaaacaa gcgcaccact tctttcgccg
    51181 gaatccccac gccggtatcg cgaacacgga tagagagata atcgccatca gcgcgaacat
    51241 gcaaaactat acagccggta tcggtgaatt ttatggcgtt actcaacagg ttggagatga
    51301 cctgctgtaa acgcatcggg tcgccattta aggccactgg cacatccggt tcaataaagc
    51361 agtacaagcc taactgcttg cgtaccacca gcggtaaata gttggcggtg atgtggttca
    51421 tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat tcaatcttcg
    51481 agaaatcgag aatatcgctg ataattttca acaacaggct ggaagagttg ttcattgccg
    51541 tcaccagacg atcgacgcct ttcggtaact ctttggtttg caacagatcc aggttaccga
    51601 taatgccata cagcggcgtt cgcagctcat gactgacggt ggcaaggaac atcgattttg
    51661 actggctcgc ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc
    51721 gcgaagaaac atccaccagc acacaaatgg ccacgttttc attacgatag cgcgaatgga
    51781 cgaagctgat ttgcagattg gtattgttgc tggtcaggac atcaacaaaa ttgacctgct
    51841 gcccacagat aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata ttgagatagg
    51901 tatgcgccag ttcgttactt aaaatattga cgccatcagc ggtacgcaaa atgcagatac
    51961 ccactggcgc ggaggcgaca atcttgcgat tgaactgctc atgttcttcc agtcgcaggg
    52021 cgtcgctttc cgccggaatg aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg
    52081 cagctccggc aagcacattc agcaaaattg cgttaaggat caacatgcga atgcgttcca
    52141 gcaccttatc aaccggcacc gaatacacga tgcttagcga tgagggtggc agatttttct
    52201 tcagcaccag ctcccggaac ccttccgtat agccaaacca ggagcgttcc tgcatccagc
    52261 gaggatcgcc cttaatttta ctttctggtc cggtaagcga aatcagggta tgaccatttt
    52321 catcaagaat ggtaaccccc atcggcaacg tacccggtaa gaaaaagttc tccatccgga
    52381 tggtctgctc gacacccaaa agcgcctgca accggttcgc cagataaact ggcgtcaacg
    52441 cgtaaaaata cccgacgcaa gggcgcggac cttcgctgat ccagtagagg ttactgccgc
    52501 tatcatcttg tggtgcattt cgatatttat tgatgcgttc atgcaacgct ttcaacgcgg
    52561 tatcgcgttc cactggcata tcacgcagac cgaaattggc catgcagagg ttatcactgc
    52621 cgattaaaaa tacccggttg agatcgtaag ccgcagaaaa attatcgcgc cagtagcgca
    52681 taaaccacgc caatgactcc agagaacctc gccaggtgtt actcattgcg gaacaatcgg
    52741 agtcggcaaa cagcggttca aacgcaggca catccgcctg cgtttctcgt ccacgcgggg
    52801 aaagcacacc gttttctgcc gataagcgat tttcggcgat gtacttaagc tctttcatca
    52861 catcagaggt gcgttgaata aagcgctgag cctgatcgga actcagatta aattcctgac
    52921 gaatttccga ttctcgctga tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa
    52981 tcaacagcca gagcactaac gccaatgctc tgaacatgta gcgcgaggct ttcagggttg
    53041 tacgaaaaga agcaaggtat ttcaaagggg agaagctccg cctcaggtga ccgatggagt
    53101 gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc tgtcaccatt gcaataatgc
    53161 gtatcgctta gaaaccattg ccttaaccag gaaagccgga gtagatggac agttctcagt
    53221 acgaaaaata agaaatgacc aatctgccga cgcttgttaa tggttatatc ctggcgtcgg
    53281 cagatcgccc agcagtctta tctgaaaaaa gagagagcca ggttgtgtga agggatggat
    53341 caccagtcaa aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat
    53401 gtttcctgtt tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtcgtc
    53461 aattccgttt cgctgcgccc accatagtag ttgatataca cctgatagcg cccgtgaact
    53521 ggtgctggca tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca
    53581 ccactgtttt tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca
    53641 tggagatcga gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga
    53701 atagttcctg tacccggcgt tgagtaaaac tgcatttttt gccggctttg cccatccggg
    53761 ctgatgacct gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca
    53821 tcggattcaa ttctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact
    53881 ttacccgccg cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca
    53941 tctgccatgt tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg
    54001 ccctctgccg gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca
    54061 ctgtgagcga caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca
    54121 ttagtccatt aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg
    54181 ataaccatca aaggccaagt gcagatactc atgggttaaa tccagccgat cctgaagcgt
    54241 aaagaagtta cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac
    54301 taaacgacag accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg
    54361 ccgccactgc ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt
    54421 tgagcgtgga gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg
    54481 gtcgtaacgc tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc
    54541 ggtagcgcga ctgccatgat aatgaaccgg atcgcctgca tagatgagct cctgcgtcca
    54601 ggcggtcatc gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct
    54661 gctgtcgggg atggtcaggc aatcaccttc ccgattggcg ttttgctgta aaaaagtacg
    54721 tatcgccaca gtcatggctt tcgctgcctc aggtggcgta cttttcgcct cgcgatccag
    54781 aactctggca acgtactctt cacgatcgag atgcgactgc aatttcagtt tgcccttttc
    54841 gctcagtaag gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tagtaaacgt
    54901 cacacgatag cgcccattaa gtacgctggg ttttactgcc gtcgtactct tttccgctgt
    54961 gattttcttc agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac
    55021 ctgcgtcggc actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt
    55081 gctggtgcca ctgccagtaa cccacagcgg tgtaccatcg gttaaccagc cagcaaaacc
    55141 accctgacgg atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg
    55201 actaccgagc atggatgcga caccgatttt cgcctcgtcc agcaccacat ccagtaagac
    55261 ttcctgagct ttgttttgcg caggtaacgt cgctaatgaa tcaagcagcg atttaaccgt
    55321 taccgaggtt tctggcttca gcgttgtaag agacgctaac cacgcaggtg cctgacgctg
    55381 ttgccagtat tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag
    55441 cccacaggaa cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata
    55501 aacctcttcc ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc
    55561 cagccaggcg taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg
    55621 taatgctgag actttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc
    55681 accgcgcagc accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc
    55741 caatagccag acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact
    55801 ccctgccgcg acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag
    55861 aacgaattgc cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag
    55921 ttctttcacc ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa
    55981 ttgcccctgc tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc
    56041 aacgtcagct cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg
    56101 ctcgctggta agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt
    56161 caccggctgc agagtaaagc tcatctcttc ttcaccgggg ataagccgat acaactggcg
    56221 ttcaacggtc accggaatgt tactttgttg agccgttttt gccggttcac gccagcggac
    56281 ctgcacattt gcggcgataa ttcgtcacca aaagagagaa tgtccggcac accctaacca
    56341 acccaacgcc agtcttcgcc ccctccagtt aacttatgtt tagcccatgc gcccgcaggc
    56401 gcaggcaaaa caactggagg cattgtcgcc atatatttcg ccagccagtt catggccagc
    56461 gcgcgctcaa tagtggattg ctcagcggtc aaaccattaa aatagcggcc gtctgggtag
    56521 cgtcaccacc gctattgagc agcacaaccg tgtgcgccag cggctgattg ctgtttgccg
    56581 ctttatccca cgcatattgc tgaacctgtg gcatcaccgt ggacttcaac tgcgcttttt
    56641 tcagcaacgt catggttaag acgtttgcca ccgcatctgc cagtggagat tccggtgtat
    56701 cgaggatcag gctgtcattg atatcgcgga tgtcttcctc agagaaatct tcatctttag
    56761 ttccgcgccg ggcgatagct tcatcccccc ctttcaacaa cgttttgcac ggcagattca
    56821 tctcctgtgc ccacgccagc accagcgccc gatgcaataa cggcatatta tccgcctgct
    56881 cggcataact gtcgagcata tgctgccagt attccggttg ttgcgttacg ccgagcgcct
    56941 ggctggcctg ccagtcggcg taccatgccc atgccgtaag gaaggcgtca ccattgccat
    57001 cttcacccca ccaggtaaag cgcgctccgg gccccgccag ttgcatcagc cgcagacggt
    57061 tatcctgaat catctgacga atgtcgttag cggcggcact ttgatgatcg gcaagcgaac
    57121 gccaggcgag actgagcggg atcagacggc taccggtgtt gatcacccca ccccacggtt
    57181 catcgactaa cgcatcaaga ttgttgcgga aaatctcctg cggcgtttca ctactttgta
    57241 gccggatatt actcgcctgc tcgggcaaca tcagcgcgtt ctcgccaccg ccgagcatga
    57301 catttttctg ctgttcaacg ggccagctgt tgtccacaaa agacagtttt gtgctaatgc
    57361 tgtcctgcac ttgcccattt tgttgcagtt ctgcacttaa caagccagat tgctgaatat
    57421 tctgcgtcag cgaaatataa ttcgcccctt tgtgcagcgt cagcgtctgg cgcatctcag
    57481 cgcctgcaaa tttagtcacc agcgccaccg gttcgttatc ctgctgactg aagataaaca
    57541 gtcctgccgc cggtttgtcg cccacgcgat acaccgttgg catactccac ttcatgtaga
    57601 gatttttttc cgaacgcaga taagcacgcc cctgcccgac cagcccgtcg ccgttcatcc
    57661 cacgcgcggt gatacgccag cgggttaacg aatcaggcat caggaacgtg aaatacgctt
    57721 tgccttgttt atcggttgtg agtgacggca tccatgccgc ggtatccacc tcttcacgcc
    57781 gtggacgttc aagcattttt actcgccgct cgctgcggtt agtcgcgcca ggcgcaaccg
    57841 gctcgctgga gagcgcctgg tcatagctga taaacgacag actggagctg gtacgcacat
    57901 tgttacgccc cagcggatag aaaaatttgc cgatattcgg cgcgatttct ggttgcagcg
    57961 cgtagatcat ttcatcgacc acgcctaccg ttagctgcgc agaaacaggt ttacctttca
    58021 gcgacgaggt taatt
//
LOCUS       DAJTRM010000015        55418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRM010000015 DAJTRM010000000
VERSION     DAJTRM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.015"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO322_000982"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO322_000982"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5905954.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO322_000983"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO322_000983"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5905955.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO322_000984"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO322_000984"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5905956.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO322_000985"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO322_000985"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5905957.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO322_000986"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO322_000986"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5905958.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO322_000987"
     CDS             7826..9559
                     /locus_tag="OO322_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5905959.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO322_000988"
     CDS             9708..10559
                     /locus_tag="OO322_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5905960.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO322_000989"
     CDS             complement(10546..10887)
                     /locus_tag="OO322_000989"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5905961.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO322_000990"
     CDS             complement(10889..11767)
                     /locus_tag="OO322_000990"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5905962.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO322_000991"
     CDS             complement(11733..14030)
                     /locus_tag="OO322_000991"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5905963.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO322_000992"
     CDS             complement(14081..14401)
                     /locus_tag="OO322_000992"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5905964.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO322_000993"
     CDS             complement(14416..15495)
                     /locus_tag="OO322_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5905965.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO322_000994"
     CDS             15804..16907
                     /locus_tag="OO322_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5905966.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO322_000995"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO322_000995"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5905967.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO322_000996"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO322_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO322_000997"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO322_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO322_000998"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO322_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO322_000999"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO322_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5905968.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO322_001000"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO322_001000"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5905969.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO322_001001"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO322_001001"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5905970.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO322_001002"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO322_001002"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5905971.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO322_001003"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO322_001003"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5905972.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO322_001004"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO322_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5905973.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO322_001005"
     CDS             30120..30728
                     /locus_tag="OO322_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5905974.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO322_001006"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO322_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5905975.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO322_001007"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO322_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5905976.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO322_001008"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO322_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5905977.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO322_001009"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO322_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5905978.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO322_001010"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO322_001010"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5905979.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO322_001011"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO322_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5905980.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO322_001012"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO322_001012"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5905981.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO322_001013"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO322_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5905982.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO322_001014"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO322_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5905983.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO322_001015"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO322_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO322_001016"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO322_001016"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5905984.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO322_001017"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO322_001017"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5905985.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO322_001018"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO322_001018"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5905986.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO322_001019"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO322_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5905987.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO322_001020"
     CDS             complement(44843..45142)
                     /locus_tag="OO322_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5905988.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO322_001021"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO322_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO322_001022"
     CDS             45895..46494
                     /locus_tag="OO322_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5905989.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO322_001023"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO322_001023"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5905990.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO322_001024"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO322_001024"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5905991.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO322_001025"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO322_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5905992.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO322_001026"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO322_001026"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5905993.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO322_001027"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO322_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5905994.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO322_001028"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO322_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5905995.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO322_001029"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO322_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5905996.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO322_001030"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO322_001030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5905997.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO322_001031"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO322_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5905998.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAJTRM010000016        53154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRM010000016 DAJTRM010000000
VERSION     DAJTRM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.016"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..265)
                     /gene="atl"
                     /locus_tag="OO322_001032"
     CDS             complement(<1..265)
                     /gene="atl"
                     /locus_tag="OO322_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein"
                     /protein_id="HCR5905999.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMV"
     gene            428..524
                     /gene="ffs"
                     /locus_tag="OO322_001033"
     ncRNA           428..524
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO322_001033"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            644..997
                     /locus_tag="OO322_001034"
     CDS             644..997
                     /locus_tag="OO322_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5906000.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1039..2589)
                     /gene="pdeB"
                     /locus_tag="OO322_001035"
     CDS             complement(1039..2589)
                     /gene="pdeB"
                     /locus_tag="OO322_001035"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5906001.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2753..3223)
                     /locus_tag="OO322_001036"
     CDS             complement(2753..3223)
                     /locus_tag="OO322_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5906002.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3339..3890)
                     /gene="maa"
                     /locus_tag="OO322_001037"
                     /pseudo
     CDS             complement(3339..3890)
                     /gene="maa"
                     /locus_tag="OO322_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4062..4280)
                     /gene="hha"
                     /locus_tag="OO322_001038"
     CDS             complement(4062..4280)
                     /gene="hha"
                     /locus_tag="OO322_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5906003.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4306..4680)
                     /gene="tomB"
                     /locus_tag="OO322_001039"
     CDS             complement(4306..4680)
                     /gene="tomB"
                     /locus_tag="OO322_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5906004.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5225..8374)
                     /locus_tag="OO322_001040"
     CDS             complement(5225..8374)
                     /locus_tag="OO322_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5906005.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8397..9590)
                     /gene="acrA"
                     /locus_tag="OO322_001041"
     CDS             complement(8397..9590)
                     /gene="acrA"
                     /locus_tag="OO322_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5906006.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9732..10379
                     /gene="acrR"
                     /locus_tag="OO322_001042"
     CDS             9732..10379
                     /gene="acrR"
                     /locus_tag="OO322_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5906007.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10507..13869
                     /gene="mscK"
                     /locus_tag="OO322_001043"
     CDS             10507..13869
                     /gene="mscK"
                     /locus_tag="OO322_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5906008.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14081..14242)
                     /gene="rsmS"
                     /locus_tag="OO322_001044"
     CDS             complement(14081..14242)
                     /gene="rsmS"
                     /locus_tag="OO322_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5906009.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14256..14783)
                     /gene="priC"
                     /locus_tag="OO322_001045"
     CDS             complement(14256..14783)
                     /gene="priC"
                     /locus_tag="OO322_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5906010.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14853..15230
                     /locus_tag="OO322_001046"
     CDS             14853..15230
                     /locus_tag="OO322_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5906011.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15383..15934
                     /gene="apt"
                     /locus_tag="OO322_001047"
     CDS             15383..15934
                     /gene="apt"
                     /locus_tag="OO322_001047"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5906012.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16063..17988
                     /gene="dnaX"
                     /locus_tag="OO322_001048"
     CDS             16063..17988
                     /gene="dnaX"
                     /locus_tag="OO322_001048"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5906013.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17321..17385
                     /gene="dnaX"
                     /locus_tag="OO322_001048"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18041..18370
                     /locus_tag="OO322_001049"
     CDS             18041..18370
                     /locus_tag="OO322_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5906014.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18370..18975
                     /gene="recR"
                     /locus_tag="OO322_001050"
     CDS             18370..18975
                     /gene="recR"
                     /locus_tag="OO322_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5906015.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19085..20959
                     /gene="htpG"
                     /locus_tag="OO322_001051"
     CDS             19085..20959
                     /gene="htpG"
                     /locus_tag="OO322_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5906016.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21140..21784
                     /gene="adk"
                     /locus_tag="OO322_001052"
     CDS             21140..21784
                     /gene="adk"
                     /locus_tag="OO322_001052"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5906017.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21916..22878
                     /gene="hemH"
                     /locus_tag="OO322_001053"
     CDS             21916..22878
                     /gene="hemH"
                     /locus_tag="OO322_001053"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5906018.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22875..23834)
                     /gene="aes"
                     /locus_tag="OO322_001054"
                     /pseudo
     CDS             complement(22875..23834)
                     /gene="aes"
                     /locus_tag="OO322_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            23986..25290
                     /gene="gsk"
                     /locus_tag="OO322_001055"
     CDS             23986..25290
                     /gene="gsk"
                     /locus_tag="OO322_001055"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5906019.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25423..27099)
                     /locus_tag="OO322_001056"
     CDS             complement(25423..27099)
                     /locus_tag="OO322_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5906020.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27337..28557)
                     /gene="fsr"
                     /locus_tag="OO322_001057"
     CDS             complement(27337..28557)
                     /gene="fsr"
                     /locus_tag="OO322_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5906021.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28775..30427
                     /gene="ushA"
                     /locus_tag="OO322_001058"
     CDS             28775..30427
                     /gene="ushA"
                     /locus_tag="OO322_001058"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5906022.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30464..30943)
                     /gene="ybaK"
                     /locus_tag="OO322_001059"
     CDS             complement(30464..30943)
                     /gene="ybaK"
                     /locus_tag="OO322_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5906023.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31147..31941)
                     /locus_tag="OO322_001060"
     CDS             complement(31147..31941)
                     /locus_tag="OO322_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5906024.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32079..32420
                     /locus_tag="OO322_001061"
     CDS             32079..32420
                     /locus_tag="OO322_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5906025.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32635..35139)
                     /gene="copA"
                     /locus_tag="OO322_001062"
     CDS             complement(32635..35139)
                     /gene="copA"
                     /locus_tag="OO322_001062"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5906026.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35401..36333
                     /gene="glsA"
                     /locus_tag="OO322_001063"
     CDS             35401..36333
                     /gene="glsA"
                     /locus_tag="OO322_001063"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5906027.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36336..37628
                     /locus_tag="OO322_001064"
     CDS             36336..37628
                     /locus_tag="OO322_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5906028.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37753..38160
                     /gene="cueR"
                     /locus_tag="OO322_001065"
     CDS             37753..38160
                     /gene="cueR"
                     /locus_tag="OO322_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5906029.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38161..38619)
                     /locus_tag="OO322_001066"
     CDS             complement(38161..38619)
                     /locus_tag="OO322_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5906030.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38616..39533)
                     /locus_tag="OO322_001067"
     CDS             complement(38616..39533)
                     /locus_tag="OO322_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5906031.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39679..40356
                     /gene="fetA"
                     /locus_tag="OO322_001068"
     CDS             39679..40356
                     /gene="fetA"
                     /locus_tag="OO322_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5906032.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40343..41122
                     /gene="fetB"
                     /locus_tag="OO322_001069"
     CDS             40343..41122
                     /gene="fetB"
                     /locus_tag="OO322_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5906033.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41185..42039)
                     /gene="cnoX"
                     /locus_tag="OO322_001070"
     CDS             complement(41185..42039)
                     /gene="cnoX"
                     /locus_tag="OO322_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5906034.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42100..42909)
                     /gene="ybbO"
                     /locus_tag="OO322_001071"
     CDS             complement(42100..42909)
                     /gene="ybbO"
                     /locus_tag="OO322_001071"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5906035.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42899..43521)
                     /gene="tesA"
                     /locus_tag="OO322_001072"
                     /pseudo
     CDS             complement(42899..43521)
                     /gene="tesA"
                     /locus_tag="OO322_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43492..44178
                     /locus_tag="OO322_001073"
     CDS             43492..44178
                     /locus_tag="OO322_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5906036.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44175..46590
                     /locus_tag="OO322_001074"
                     /pseudo
     CDS             44175..46590
                     /locus_tag="OO322_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47303..48397)
                     /gene="mnmH"
                     /locus_tag="OO322_001075"
     CDS             complement(47303..48397)
                     /gene="mnmH"
                     /locus_tag="OO322_001075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5906037.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48466..49392)
                     /gene="allS"
                     /locus_tag="OO322_001076"
     CDS             complement(48466..49392)
                     /gene="allS"
                     /locus_tag="OO322_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5906038.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49622..50104
                     /gene="allA"
                     /locus_tag="OO322_001077"
     CDS             49622..50104
                     /gene="allA"
                     /locus_tag="OO322_001077"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5906039.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50182..50997
                     /gene="allR"
                     /locus_tag="OO322_001078"
     CDS             50182..50997
                     /gene="allR"
                     /locus_tag="OO322_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5906040.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51087..52868
                     /gene="gcl"
                     /locus_tag="OO322_001079"
     CDS             51087..52868
                     /gene="gcl"
                     /locus_tag="OO322_001079"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5906041.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52881..>53154
                     /locus_tag="OO322_001080"
     CDS             52881..>53154
                     /locus_tag="OO322_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5906042.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12818 a        13674 c        13999 g        12663 t
ORIGIN      
        1 ataccatcac accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggcccggtta
       61 gcgaaattgt gccatggcga ttaaccaccc ggtgccaggg taaggtgctg ccttcaggga
      121 gacgctttaa cacaccgccc acctggcgcg cggcgcgggg cgatcccgcc agtttcgcca
      181 catcaccgta agtggtgaca tagccttcgg gaatagcggc gacgatttgc cagacgcgtt
      241 ggggaaatga atcttctttt tccatctttt cttcctgagg taatttttca gcataatctg
      301 gaaaaacgcc cgagtgaagt cgcattgcgc aagaaaccag catctggcac gcaatgggtt
      361 gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt ggttttcaac gctctcaatg
      421 ggggctctgt tggttctccc gcaatgctac tctgtttacc aggtcaggtc cggaaggaag
      481 cagccaaggc agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg
      541 cctcccaccg tttcgtcaaa aaatcccagc atggctaaac tttaaccaca actgacgtcg
      601 caagaattgt ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg
      661 ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc
      721 cgcgccattg tttaaagagt ttggcgcgct tcgtattgtc gaatgctggg ccagcgatgt
      781 gccggatggc aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt
      841 tgtctttagc tggattgaat acccttcaaa agaggtccgc gacgcagcta atcaaaagat
      901 gatgtcggac ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat
      961 gatctatggc ggattcgagt caatcatcga cgaataggta gcgtgacggg ctgtgcggcg
     1021 tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag cgtaagaaat cttctttcgg
     1081 taatgccttg ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtagccactc
     1141 ttcttgttta ctggtttcga ttccctccgc taccattttc agtttcagtg tttttgccat
     1201 ttcgatgata tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat
     1261 cttcagaatg tcgacatcca gatcctgtaa ataacttaaa cttgaatacc ccgtaccaaa
     1321 atcatcaaga taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt
     1381 tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg
     1441 atagtgattg atcatttcac gcagcaattg cgggattttt tccgaggtga gcacggtgga
     1501 ttcaagattg atcgaaatat gctgctgtgg atgctgacgc aagcagtcgc ccatatcttc
     1561 aaagacgctt cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag
     1621 cggaataaaa ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc
     1681 ctcagcaccg acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat
     1741 atcacgattt tcgatagcat cctgcagccg atgatgcggt gattggatac ggcgcaaaat
     1801 acgcagcaca aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa
     1861 gacttgccga tgccagcctt tctgcaacat tttcgttgaa gcccaggtga tgatcgaaat
     1921 attcatctcc aggaagggca gtatatcgta aatgatccca ttattttcga tatgctcacc
     1981 gggtgttttt tgtagcctgg taataattcc ctgagcaatt tcatcgctgc tggttatgac
     2041 aacgttatgg gcattgccaa taatggcggc atcaatttgc catgaactat agggaatgac
     2101 atcaataaag gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat
     2161 gtaacggata atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga
     2221 aattttacct agctcgggaa aggtatcggg cggactttca tgctccagag acgaacactg
     2281 gggaacgttg ttatcgatat aaaccacttc ctgaatatag cgataactgt aagatacccg
     2341 acgcatttcc atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcctg
     2401 cagcgcatct ttcccttgtg tcgccacctt attggctcga atagcgacgc gggaggaata
     2461 ggtatccagc tcttcaataa acgatgtttc tacctgctga tgggccagcc agatgcttaa
     2521 gccgacaggc agcaatactg aaagaatcag tactcccgaa ataaggccga ccagatgtcg
     2581 tgttctcacc agcatatcct tattaacaca gtgtagggaa agtatatctg attgtggaaa
     2641 aaggctggca ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaaacga
     2701 tcattgtcat ttgaccaggc gcagagcttt acgcctggta tgtaacccta acttaagatg
     2761 ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga cagttcacct ttacggacga
     2821 tcattttttg cagttgttcc ttatgaacat ccgcgacacg cggagagttc aagatggctt
     2881 caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga
     2941 aatccagcac gtcgatatct tcatagcggt agcgtgggta gacatcgaaa aagaagaaac
     3001 cattaagcag gataaataat acaaccagta gccagacaga gcctgacagc gtttcggact
     3061 gcaacatcac cgccagggtg gcaaaaaaag caacgtacat accgataaac agccccggat
     3121 gtttacggat aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca
     3181 gagactcaat cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtggta
     3241 tctgcaactt tccagggaac accctatttt aacgtgggaa tagtagaggg gaagtaacag
     3301 atctaccaca attttgcata acagttgcga aaaaccgatt acaatttttt aattattctg
     3361 gctggattac cgcccacgac aacgttgtcc gggacacctt ttgtgacaac tgcacctgaa
     3421 gctaccacga cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg tccgccaatc
     3481 cagacgttat taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca
     3541 gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca
     3601 ccgatgcgaa tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattattg
     3661 ccgagaaaaa tgttatagcc ataatcacag cgaaacgttg gctcaatata agcctctgtc
     3721 acctgaccga ataaattagc gagaatttac tggcgtaatg tgtgctcttc cgccagggaa
     3781 tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa cgtctcatct
     3841 gccgagcgat acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc
     3901 cggttgctga aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttctcat
     3961 tacatctgtg tgataaagat cacatagggt atgtcttcat ggcgaaaaag tataaaattc
     4021 ttaataaaca gccggttata gcaccgaaag cgaattattt attagcgaat aaatttccat
     4081 actgaggaag ggatcttgtc gtacagttta ttcatggtca attcggcgag gcggtgatct
     4141 gcggctgagt aaaataccgc cagttcatta tctgataatt cgtatttatt tttctcgata
     4201 acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata atcggttttt
     4261 gttaaaggtt tttcggacat acttctaccc atggttgtaa taattctaac aagataaact
     4321 cgcaggattc tctttcgtcg cgttgacaaa acaacggaat agtcgattac ctgacttccg
     4381 ccatttctga agagcctgca tattaatacc ataactgctg aacaacataa aggtgtcatc
     4441 cagatattcg tcgatctgct caatgagctt attgtcttca ttatacttaa ttttgtaatt
     4501 aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac tggaggttga tcgccgaggt
     4561 tgggtcgtta acccaaccat gattgctttc ttcaaggttt gcaagacagt catgatacag
     4621 ggtttcacag agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt attcatccat
     4681 aacgcgtccc cttcttagtg gttgaactaa cggacacctt tcgggatgga aaaaacttac
     4741 tgacctagac ttgccctctc gtttgttagc ttaactataa gccactcttt gcaggttttc
     4801 atcgcattat tacgaaacat tacaattagg aaggaatcgg gacaggactc actttttcat
     4861 actagcacta acccgcagca ggtaaaagca gtttgctcga actgttcata acatttcagg
     4921 attatcgcca gatgaaattg gtgacactaa taccaggatt gctctgaata tgacgtaata
     4981 accgaggaat gaataaagaa ttaccgcaaa taattaagaa tagcctctaa atgattatgg
     5041 attattaaat ctattagcgc tacttaatat aaattaacta acaatcagta ttttttatga
     5101 attttatacg tggttaatac tggttttcgt atgagatcct gagttggtgg ttcaattact
     5161 ccttaatgtt cgtaggttat gcataaaaag gccgcttacg cggccttagt gattacatgt
     5221 tgtatcaatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg cggctaaagc
     5281 ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc gttgcggtca
     5341 ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca gcaccagtac
     5401 tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg atcggacgta
     5461 aacgcatccg caccgcatca agcgtcgctt caatcagacc tttaccttct ttatccatca
     5521 agtctttggc gaattcgacg ataagtatcg cgttcttcgc cgacaaccca atggttgtga
     5581 gcagtcctac ttggaagtaa acgtcattgg tcaggccacg gaaggtggca gccagcaacg
     5641 caccgataac ccccagcgga acgaccagca taacggagaa cggagtcgac cagctctcgt
     5701 acagcgccgc cagacacagg aatacgacaa tcaacgaaat cgcgtacagt gaaggtgcct
     5761 ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagcca acaccggtag
     5821 gcagtttgct cgccagttgt tccatcagct ccattgcttc accggtactt ttacccggtg
     5881 ccgcctggcc taagatttcc atggatggca ggccgttgta acgttccaga cgcggcgaac
     5941 cgtactccca acgagaagag gagaacgccg agaatggcac catctgacca tcagcagcac
     6001 gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct gacatgacgt
     6061 aaactttctt cacacgaccg cggtcgataa agtcgttcac atagctaccg ccccatgcag
     6121 cgcccagagt ggtgttaatg tcgttgatag aaacacccag cgcctgcgct ttttcctggt
     6181 cgatatcaat cttaaactgc ggggtatctt ccagaccgtt tggacgtacg ctggtcagca
     6241 tatcagggtg cttcgctgct tctgcaagca gctggttacg cgcctgagtc agtttttcgt
     6301 gaccaaggcc agcctggtca atcagctcaa agtcaaagcc ggttgcagta cccagttcca
     6361 cgattgcggg caggttaaag gcgaaaacca tcgcatcttt gatttgcgag aaagcgcgtg
     6421 ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg gcccagtcct
     6481 tcaaggaaac gaacgcaata ccggtattct gaccacgtcc cgcaaagccg aagccgttaa
     6541 cggcgaacac cgactcaacg ttgttctttt ctttggtcag atagtaatgc gttacctcat
     6601 tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg cagctgaacc atggtcataa
     6661 atacgccctg gtcctcatct ggcaagaagg agcttggcag acgcacgaac agataggcca
     6721 tgccgaccac gatgatcaga tacagcacta ggtaacgccc cgtactgcgc agaataccgc
     6781 ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat gcggttcaac cagccgaaga
     6841 agcctttttt accttccccg tgatcgcctt tggcaatcgg tttcagcatg gtggcacaaa
     6901 gagccggagt caggatcaac gctaccagta ccgacagcgc cattgctgaa acaatggtaa
     6961 tagagaactg acgatagata gcaccagtag aaccgccaaa gaaggccatc ggtacgaata
     7021 ccgccgacag taccatcgcg ataccgacca gagcgccctg aatctgcccc atcgacttac
     7081 gggtagcttc ttttggcggc aaaccttctt ccgccataac acgctccacg ttttctacca
     7141 caacgatagc gtcatccacc aacaagccga tggcgagcac catcccgaac attgttagcg
     7201 tgtttatcga gaagccaaag gcggcaagga cggcaaaggt cccgagcaat accaccggta
     7261 cggcaatggt cggaatcaac gtcgcgcgga agttctgcag gaacagatac ataaccagga
     7321 acacgaggat gatcgcttcg accagcgttt taaccacttc gtgaatagag attttcacga
     7381 acggcgtggt gtcgtacggg taaacaattt tcagacccga cgggaagaac ggttccatct
     7441 tcgccagttc agcacggatt gccgcagcgg tatccagcgc gtttgcaccg gtcgccagct
     7501 tgatccccag accggaagca ggttggccgt taaactctgc gatgatgtcg tagttctcac
     7561 cacccagctc aatcttcgcc acatcacgca gcagtacgcg ggaaccatcc tgattcactt
     7621 tcagcaggat tttgccgaac tcttcagtag aggtcagacg cgtctgagca ataatagagg
     7681 cgttaagctg ttggcctttc accggcggcg taccaccgag ctgacccgcc gcaacctggg
     7741 cgttctgcgc tttgatggcg gtaatgacat caaccggcgt tagctggaat ttgttcagct
     7801 cattcgggtt catccagata cgcatcgcgt actgtgaacc gaacaactga acatcaccca
     7861 cgcccgacgt acggctgatg gcatctttca tattcgccgc tacgtagtca gagatatcct
     7921 cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac catcaggaag ctgctggatg
     7981 atttctcaac gctcacccct tgctgctgaa cttcttgcgg cagcaacggc atcgccagct
     8041 gcagtttgtt ctgcacctga acctgcgcga tatccgcatc agtaccagac tcaaaggtca
     8101 gggtgatctg cacggtaccc gtggagtcac tgttagagga catgtacatc aggttatcga
     8161 taccgttcat attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt ttcgcatcag
     8221 cgccggggta ggaggcggag atcgttactg ccggcggtgc aatcgtagga tattgcgcca
     8281 ccggcagttt gaggatcgcc agcccccctg ccaacatgat gataatggcg atcacccacg
     8341 caaaaatcgg gcgatcgata aagaaattag gcatgtctta acggctcctg tttaagttaa
     8401 gacttggact gttcaggctg agcaccgctt gcggcttgct ggttattatc agcggtaact
     8461 tcttgtgctt ttacctggac accaggacgc actttctgca gcccacttat tactacgcga
     8521 tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc caatagcctg gcttgcaacg
     8581 atcggacggg tttccacttt gtcatccgcg ccaactacca gtacggtggc atcaccacgc
     8641 ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt ttggattaag cccttcttcc
     8701 agacgtgcgc gcacgaacat acccggcagc agagtgtgat ccgggttcgg gaagatagcg
     8761 cgtagggtga tagacccagt ggtctgatca acggtaacgt cagagaattc cagcgtaccg
     8821 tcctgcggga acttaatgcc gtcactggtg atcagcgaca ctttggcttt gccgttctct
     8881 tgtttcagcg tgccattcgc cagttcctgt ttcaggcgaa ggaagtcgtt gctggactgg
     8941 gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg ccagcacagt cgcctgaccg
     9001 ttctgtacca atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct aatcggagag
     9061 gtgactttgg tgtaagccag attgatccgc gcagtttcaa cggcagcttt cgccgcagtt
     9121 accgcagcat tcgcctgttg cgcatcagcc agagcctgat cgtacccttg cttactgatg
     9181 tactgagtac cgagcagttt ctgataacga ttcaccgtca attgcgcgat attggctgca
     9241 gcctgggctt tcgccagatc acctttcgca ctgtcgtatg tcgcctgata ggtcgcagga
     9301 tcaatctgat agagagagac accagcttcg atgtcgctac cttctttgaa attacgcttc
     9361 aggataatcc cgctaacttg aggacgaact tctgcgatcc ggtaggcact ggtgcgacct
     9421 ggaagctcgg ttgtgatctg cagaggttca gttttgactg ttactacgcc aacggcgggc
     9481 atctgctggc caccttgttg ggcctgtttg tcgtcacatc ctgttagggc taagctgcct
     9541 gagagcatca gaacgaccgc cagaggcgta aaccctctgt ttttgttcat atgtaaacct
     9601 cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa taaacccatt gctgcgttta
     9661 tattatcgtc gtgctatggt acatacattc acaaatgtat gtaaatctaa cgcctgtaaa
     9721 ttcacgaaca tatggcacga aaaaccaaac aagaagcgca agaaacgcgc caacacatcc
     9781 tcgatgtggc tctacgtctt ttctcacagc agggggtatc atccacctcg ctgggcgaga
     9841 ttgcaaaagc agctggcgtt acgcgcggtg caatctactg gcattttaaa gacaagtcgg
     9901 atttgttcag tgagatctgg gaactgtcag aatccaatat tggtgaacta gagcttgagt
     9961 atcaggcaaa attccctggc gatccactct cagtattaag agagatatta attcatgttc
    10021 ttgaatccac ggtgacagaa gaacgacgtc gattattgat ggagattata ttccacaaat
    10081 gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca acgtaatctc tgtctggaaa
    10141 gttatgaccg tatagaacaa acgttaaaac attgtattga agcgaaaatg ttgcctgcgg
    10201 atttaatgac gcgtcgggca gcaattatta tgcgcggcta tatttccggc ctgatggaaa
    10261 actggctctt tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat tacgtcgcca
    10321 tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact aacgaataac
    10381 cctgaatctg actccaggat ttttcctgga cattttcgtc gttgctattc tggttcactg
    10441 cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc ttcaggttca gaaaccttca
    10501 ttcatcatga ctatgttcca gtattacaaa cgatcacggc attttgtttt ttcagcattt
    10561 attgcttttg tttttgtctt gttatgccag aacacggcgt ttgcgcgggc gtcatcgaat
    10621 ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg actcactaaa taaacaaaaa
    10681 gatctttctg ctcaggacaa actggtgcag caggatctga cagatacatt agccaccctc
    10741 gataaaatcg atcgcataaa agaagagaca gttcagctac ggcaaaaagt cgctgaagcg
    10801 ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt cgataacgac
    10861 gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc agctggaaac tcgcgttgcc
    10921 caggcgctgg acgatttgca aaacgcacaa aacgatctgg cgtcttataa cagccagctg
    10981 gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc ttcgcagcag
    11041 ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg gcgagactgc cttacgtccc
    11101 agccagaaag tgttaatgca ggctcagcag gcgttgctga atgcggagat tgaccagcag
    11161 cgtaaaagcc tggaagggaa caccgtcttg caggatacct tgcaaaagca acgtgattac
    11221 gtgacggcga acagcgcccg tctggagcac cagttgcagc tgttgcaaga agcggtaaac
    11281 agcaagcgcc tgactttaac tgaaaaaacg gcgcaggaag ccgtctcccc ggatgaagcc
    11341 gcgcgtattc aggctaatcc gctggtgaaa caggaactgg aaattaacca gcagttaagt
    11401 cagcgtctga ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa cattaaagtc
    11461 aaaaactggc tggagcgggc gctgcaatcg gaacgcaata ttaaagagca gattgccgtc
    11521 ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac gctgccctcg
    11581 gcggatgaac tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga acagtttgaa
    11641 gttaaccagc agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa actggaagaa
    11701 ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt ggttgatatg
    11761 cgtcgcgaat tgctggatca actcaacaaa cagttgggta accagctgat gatggccatt
    11821 aacctgcaaa tcaaccagca gcagttaatg agcgtatcga aaaacctgaa atccatcctg
    11881 actcagcaaa tcttttgggt gaacagtaac cgtccaatgg actgggactg gatcaaagcg
    11941 ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa tcacggtgaa ctgggaaaaa
    12001 gcctggcctg ccgtttttat cgctttcctc gccggtttgc cgctgctgtt gattgccggg
    12061 ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac agaaactggc ttccgctgtg
    12121 ggttccctgc gtaacgatag ccagctcaac acaccaaaag cgatccttat cgacctgatc
    12181 cgcgcgctgc cggtgtgcct gattattctc gcggttggcc tgattctgtt gaccatgcag
    12241 ctcaacatca gcgaactgct atggtcgttc agtaaaaaac tggcgatttt ctggctggtg
    12301 tttggcctgt gctggaaggt actggagaaa aatggcgttg ccgtgcgtca cttcggcatg
    12361 ccggagcagc agaccagcca ctggcgtcgg caaattgtcc gcatcagtct cgcgttgctg
    12421 cctatccatt tctggtctgt ggtggcagaa ctttccccgc tgcatctgat ggatgatgtg
    12481 ctggggcagg cgatgatttt cttcaacctg ctgctgattg ccttcctggt atggccaatg
    12541 tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc gactagtcac cattaccgtg
    12601 ctgtcgatta tcccgattgc attgatggtg ctgactgcca cgggttattt ctacaccacg
    12661 ctgcgcctct ccgggcgttg gattgaaacc gtttatctgg tgatcatctg gaacttgctc
    12721 taccagacgg tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgcg
    12781 ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc gccggaagaa
    12841 cccaccattg cactggagca agttaaccag cagacgctgc gtattaccat gttgctgatg
    12901 tttgcgctgt tcggtgtcat gttctgggca atttggtccg atttgatcac cgtgttcagc
    12961 tatctcgaca gcatcacgct ctggcattac aacggcactg aagctggcgc tgcggtggtg
    13021 aaaaacgtca ccatgggcag tctgttgttt gcgattatcg cctcaatggt ggcctgggcg
    13081 ttgattcgca acctgcctgg tttactggaa gtgctggtgc tctcgcgact gaatatgcgc
    13141 cagggcgcgt cgtatgccat tactaccatc cttaactaca tcattattgc cgttggtgcg
    13201 atgacggtgt tcggatcgct gggcgtctct tgggataaac tccagtggct ggccgcagca
    13261 ttatccgtag gtcttggttt tggtttacaa gaaattttcg gtaacttcgt ctccggtttg
    13321 atcattctgt tcgaacgtcc ggtgcgtatt ggcgatacgg taaccattgg tagcttctcg
    13381 gggacggtaa gtaagatccg tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa
    13441 gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga tcaactggtc gttgactgac
    13501 actactacgc gtctggtgat ccgtctcggc gtggcctatg gctccgatct ggaaaaagtg
    13561 cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg tgatgcacga accaatgccg
    13621 gaagtcttct ttacggcatt tggtgccagc acgttggatc atgagctgcg tctgtatgtg
    13681 cgtgaactgc gtgaccgtag ccgtactgtc gatgagctga accgtactat cgatcagctg
    13741 tgccgtgaaa acgacatcaa cattgccttt aaccagcttg aagtgcatct gcacaacgag
    13801 aagggcgatg aggtgacgga agtaaaacgc gactacaaag gcgacgatcc gacaccagcg
    13861 gttgggtaat aaccaaaggg ggcgacattt agttgccccg agattactga caaagtgcgc
    13921 gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg caagaattca
    13981 atatattgca ggagcggtgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
    14041 cccgcaatct taggggacat ttagcggccc catttatttc tcacttttcc gcctcatcat
    14101 cgtgcgttaa tttcttttca taatcacgct ttacaatatc cagcgcgcgc agcacggtac
    14161 tggcagggat ctgattttcc tccagcagca caatcaaatc gacagccagt ttgacatcgt
    14221 caggggcatt ttcaagtgac atactctttc cattgctagc gggttaagcg cgctagcctg
    14281 ttttcgattt tttccagcgc atggcggcag cgtgccaggc gcgcttcata ggcttccact
    14341 tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc gcgccaggcg ggctctgcgt
    14401 tcggcaacca tctcacgtag ccgccgctca aaatcctgat gctgaatacg tttacgctgc
    14461 cagcgggaaa ttttcggtgg cgcactgtcc cactcgcgca atgaccaggc ggaggcttca
    14521 cgcgcgatgg cttccagttg tgccgccaga tgttccgcca gccaggccac ttgcggcagc
    14581 tgttgctgct caactgcatg acgaagcgca gccagattat cgcccgcctc gtcgagacaa
    14641 gactgtagtg ttgtcgcacg agtctgaaaa agatgcctgt cgaaacgagc acttagcgtg
    14701 gcgaactgtg acaccggggc acaacgctga cgcagcgtag cgagctgtcc ttccagtttt
    14761 tccagcagca gggcggtttt cacgataaga cctcaatgaa aatgataatt gttatgctaa
    14821 agtagccact atgtaagctg acaacactca atatgcaacg aatcatttta atcatcattg
    14881 gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt accggtatta ccgacgacgc
    14941 cgtttatcct gctggcggcc tggtgctttg cccgttcttc cccgcgcttt cacgcctggt
    15001 tgctgtaccg ctcatggttt ggcagctatc tacgtttctg gcagaaacat catgcgatgc
    15061 cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct cacgtttgcc atttctctgt
    15121 ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt aattctcgcc tgtttgcttt
    15181 tctatatgtg gcgaattccg gtgattgatg aaaagcaaga aaagcactga agcacaacaa
    15241 tcgcagttgc aattattgcg tacagccagt acattctggc gttttcgagc acaggcgcag
    15301 gcggtcaaag gttaaacaac agttactttt gatacgttta aaacgcgccg tgagtaccac
    15361 cgtaacaagc aggcatacac ttatgaccgc gactgcacag cagcttgagt atctcaaaaa
    15421 tagcatcaaa agcattcagg actacccaaa acccggcatt cttttccgcg atgtcaccag
    15481 cttactggaa gacccgaaag cttacgctct cagcatcgac ttgctggttg agcgttacaa
    15541 aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt ttggcgctcc
    15601 ggtagctctg ggtctgggcg ttggctttgt accggtccgt aaaccgggca aactgccgcg
    15661 tgaaaccatc agtgaaaatt acgacctgga atacggcacc gatcagctgg aaatccacgt
    15721 tgatgccatc aaaccgggcg acaaagttct ggtggtggac gatctgctgg caaccggcgg
    15781 cactatcgaa gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg ctgacgctgc
    15841 gttcattatc aacctgtttg atctcggcgg cgaacagcgt ctcgaaaaac agggcattac
    15901 cagctacagc cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt gctgcccacg
    15961 ctacggacag cacaagatgt gcattcagcc tcgccgttct gacggggctg tgttagcatt
    16021 accccttcgt gaatccacct tccagcgttt cagagcctgc caatgagtta tcaggtttta
    16081 gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca tgtgctgacc
    16141 gcactggcga acggcttgtc gttagggcgt attcatcatg cttatctttt ttccggcacc
    16201 cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga aggggctaaa ctgcgaaacc
    16261 ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga gcaggggcgc
    16321 tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacca aagttgaaga tacccgcgac
    16381 ctgctggata acgtccagta cgctccggcg cgtggtcgtt tcaaagttta tctgatcgac
    16441 gaagtgcata tgctgtcgcg ccacagcttt aacgcactgt taaaaaccct tgaagagccg
    16501 ccggagcacg ttaagtttct gctggcgacg accgatccac agaaattgcc ggtgacgatt
    16561 ttgtcacgct gtctgcaatt tcatctcaag gcgctggatg tcgagcaaat tcgccatcag
    16621 cttgagcaca tcctcaatga agaacatatc gctcacgagc cgcgggcgct gcaattgttg
    16681 gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc tgaccgacca ggcgattgcc
    16741 agcggtgacg gccaggtttc aacccaggcg gtcagtgcga tgctgggtac gcttgacgac
    16801 gatcaggcgc tgtcgctggt tgaagcgatg gtcgagggca acggcgagcg cgtaatggca
    16861 ctgattaatg aagccgctgc ccgtggtatc gagtgggaag cgttgctggt ggaaatgctc
    16921 ggcttgttgc atcgtattgc gatggtacaa ctttcgcctg ctgcacttgg caacgacatg
    16981 gccgccatcg agctgcggat gcgtgaactg gcgcgcacca taccgccgac ggatattcag
    17041 ctttactatc agacgctgtt gattggtcgc aaagaattac cgtatgcgcc ggaccgccgc
    17101 atgggcgttg agatgacgct gctgcgcgcg ctggcgttcc atccgcgtat gccgctgcct
    17161 gagccagaag tgccacgcca gtcctttgca cccgtcgcgc caacggcagt aatgacgcca
    17221 accctggtgc cgccgcaatc agcgccgcag caggcaccga ctgtaccgct cccggaaacc
    17281 accagccagg tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca
    17341 aaaaagagtg aaccggcagc cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa
    17401 agactggctt cggtcaccga tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa
    17461 gcgccagcca aaaaagaagc gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa
    17521 gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa
    17581 ctggcggtga agctagcggc agaagccatt gagcgcgacc cgtgggcggc acaggtgagc
    17641 caactttcgc taccaaaact ggtcgaacag gtggcgttaa atgcctggaa agaggagagc
    17701 gacaacgcag tatgtctgca tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca
    17761 cagcaaaaac tggctgaagc gttgagcatg ttaaaaggtt caacggttga actgactatc
    17821 gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt ggcgtcaggc gatatacgaa
    17881 gaaaaacttg cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt
    17941 cggtttttcg atgcggagct ggatgaagaa agtatccgcc ccatttgatc gtaagcacag
    18001 cttacgttcg tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct
    18061 gggtaacctg atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga
    18121 gatcgcgcag ctggaagtta ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa
    18181 cggtgcacac aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga
    18241 gatgctggaa gacctggtgg ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac
    18301 gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg gctttaagat
    18361 gccgttctga tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgtctg
    18421 ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt
    18481 agcggcggga tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc
    18541 gccgattgcc gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt
    18601 caggaaaacg gtcaaatctg tgtggtggag agtccggcgg acatctacgc cattgagcag
    18661 acggggcagt tttctggtcg ttattttgtg ttgatggggc atctgtcacc gctggacggc
    18721 atcggtccgg atgatatcgg gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc
    18781 actgaagtga tcctcgccac caatcccacg gttgaaggtg aagctaccgc taactacatt
    18841 gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt
    18901 ggcggcgaac tggaaatggt cgatggcacc acgctgtcac actccctcgc cgggcgtcat
    18961 aagattcgtt tttaagcaaa cgagagcagg agcacctgct ctcgcttgaa attattctcc
    19021 cttgtcccca tctctccctc atcctgtttt taaccttaaa atggcattat tgaggtagac
    19081 ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct
    19141 gatgatccat tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc
    19201 ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct aacccggacc tgtacgaagg
    19261 tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc tgaccatctc
    19321 cgataacggc gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa
    19381 atccggtacc aaatcattcc tcgaatccct gggttctgac caagcgaaag acagccagct
    19441 gatcggtcag tttggtgttg gtttctactc tgcgtttatc gtggccgaca aagtgaccgt
    19501 gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga
    19561 aggtgaatat accgttgccg atatcaccaa agaagatcgt ggtactgaaa tcaccctgca
    19621 tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc gtgcgttcca tcatcagcaa
    19681 atactccgac catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga aagatggcga
    19741 aaccgttatc tcctgggcaa aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc
    19801 ggaaatcacc gatgaagagt ataaagagtt ctacaaacac atcgcccacg actttaacga
    19861 tccgctgacc tggagccaca accgcgttga aggtaagcag gagtacacca gcctgctgta
    19921 catcccgtcc caggctccgt gggatatgtg gaaccgtgat cataaacacg gcctgaaact
    19981 gtacgttcaa cgcgtgttta tcatggacga cgctgaacag ttcatgccga actatctgcg
    20041 cttcgtgcgt ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct
    20101 ccaggacagc acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat
    20161 gctggaaaaa ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg
    20221 cctggtactg aaagaaggtc cggcggaaga tttcgctaac caggaagcga tcgccaaact
    20281 gctgcgtttt gcttctactt ataccgattc ttctgcgcag accgtgtctc tggaagacta
    20341 cgtttcccgc atgaaagaag ggcaggagaa aatctactac atcactgcag acagctatgc
    20401 ggcagcgaag agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct
    20461 gctttccgac cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc
    20521 gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg aagttgatga
    20581 gagcgcgaaa gaagcggaga aagcactgac tccgttcatc gaccgcgtga aagctctgct
    20641 cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgatcgt
    20701 ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg cggcgggcca
    20761 gaaagtgcca gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg
    20821 tgcggcagat actgaagatg aagccaagtt cagcgagtgg gtagaactgc tgctggatca
    20881 ggcgctgctg gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa
    20941 tcagctgctg gtttcctgat gtcatgccgg atgacctttg tgtcatccgg catttttctt
    21001 ttcatcatct gtactttccg caaattatct cgccattaac cgtttcagcc tcaggtgcct
    21061 ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa aatcgacaca
    21121 ttttaagggg attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg
    21181 gactcaggct cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat
    21241 gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga
    21301 tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca
    21361 ggaagactgc cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga
    21421 cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga
    21481 actgattgtt gatcgtatcg taggccgccg cgttcatgcg ccgtctggtc gtgtttatca
    21541 cgttaaattc aatccgccga aagtagaagg caaagacgac gttaccggtg aagaactgac
    21601 tacccgtaaa gacgatcagg aagaaaccgt gcgtaaacgt ctggttgaat accatcagat
    21661 gactgcaccg ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc
    21721 gaaagttgac ggtaccaagc aggttgctga agttcgcgct gatctggaaa aaatcctcgg
    21781 ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc ttatccacct caggcaatac
    21841 ctctcacagc aattagttct tcttcctcac ttttccgcta caattatcaa caagttgaat
    21901 cgataagagg cggtaatgcg tcagactaaa accggtatcc tgctggcaaa cctgggtacg
    21961 cccgatgccc ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga
    22021 cgcgtggttg atacctcacg gttgttatgg tggccgttgc tgcgcggcgt gattttgccg
    22081 ctgcgctcgc cgcgtgtggc gaagctgtac gcctctgtct ggatggaaga cggctcgccg
    22141 ctgatggttt acagccgcca gcaacagcag gcgttggcac aacgtttacc ggatacgcca
    22201 gtggcgctgg gcatgagcta cggctcgcca tcactggaaa gctccgtcga tgaactcctg
    22261 gcagagcatg tagatcatat tgtggtgctg ccgctttatc cgcaattctc ctgttctacg
    22321 gtcggtgcgg tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg
    22381 atatcgttta ttcgtgatta cgccgataac cacgattaca ttaatgcact ggcgaacagc
    22441 gtacgcgctt cttttgccaa acatggcgaa ccggatctgc tgctgctctc ttatcatggc
    22501 attccccagc gttatgcaga tgaaggcgat gattacccgc aacgttgccg cacaacgact
    22561 cgcgaactgg cttccgcact ggggatggta ccggaaaaag tgatgatgac ctttcagtcg
    22621 cgctttggcc gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga
    22681 gaaaaaggcg taggtcatat acaggtgatg tgcccgggct ttgctgcgga ttgtctggag
    22741 acgctggaag agattgccga gcaaaaccgt gaggtcttcc tcggtgcggg cgggaaaaaa
    22801 tatgaatata ttccagcgct taatgccacg ccggaacata tcgaaatgat ggctaatctt
    22861 gttgccgcgt atcgctaaag ctgagcggta aagaactgag cgccgtcgcg aagagcctcg
    22921 tcggcggttt tcatcatccg tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt
    22981 ttgaactcac agggctgctg atgcgccgct aacgtctagt aaagcagacg gctgtcatcc
    23041 agcagcggat cgaactccgc cccggcaata aaacagggcg gaacttcgcg agtgagatca
    23101 ttattaaaca gacagtaata cggtgactcg cggtccgcgt cgttgcttaa atacgcctct
    23161 tcatacatct gcaaatcctg ttgcgttaag ccatcccaga caccgcccaa cagacgacga
    23221 gtcacggaat cccgtaatcc gtaaagccca taccacagca aaacacccgc aattttaccg
    23281 caatcgatct gtttatcacg caaccacaac gcactggcga gcgccagcat ggcacctgcg
    23341 gaatcaccgg caaagccaat gcgggacata ttgatttgat aatcctccgc ctgctggtgg
    23401 aaataacaac aagcagccac aatttcctct atcgcttgtg gaaaacgcgc ttcaggtgaa
    23461 agggtgtaat taataccaat caccgtacat tggctgtagc ttgccagcag gcgcatgatg
    23521 cgatcgtggg tatcgagatt gccgagaata aaaccgcctc catgcaaata aaatagcgtc
    23581 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    23641 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    23701 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    23761 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    23821 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    23881 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    23941 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    24001 aaacgtaaat ccaaacatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    24061 cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    24121 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    24181 ctggtgattg aggatgacgt agccgaagtg ctttatcagg agctaaaaca gaaaaacctg
    24241 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    24301 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    24361 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    24421 gatggtccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    24481 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    24541 ggagcctcgg cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg
    24601 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    24661 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    24721 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    24781 gatccgttgt tggcatctga caaggcgctg gactgggtag agctggtgct gtgcaccgcc
    24841 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    24901 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    24961 cgccacaagg attgccagaa tccgctgcgt gtctattcgc acattgcgcc gtacatgggc
    25021 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    25081 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    25141 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    25201 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    25261 agccaggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    25321 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    25381 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacttc
    25441 acccgccggt ggcgtttcca gcagttccag catagtacgg gcaatttcac gttcacccat
    25501 cactacctga ttcgcgccac gttcggtgat atacgtcact tcatcgtcat aatgggcgcg
    25561 ggcgataatc tcaatatccg ggtttttcgc gcgggcagac gccacaatct cacccgcttc
    25621 ataaccgttg gggatcgtca ggatcagcca ttttgcacat tccagatgtg ccagttgcat
    25681 aatttcttcg ttcgctgcat tgcccaatac agcgcggacc ccacgctctc gcagctcatc
    25741 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    25801 ccccagcagg ctgcctacac gaccgtaacc caccaatagc gcatggttgc aaatatccac
    25861 tgggatctgt ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    25921 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    25981 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    26041 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    26101 cgtacgttgg gagtgaccaa acagacggac aaggaacagg gccgcgaccg atttaccaaa
    26161 cagaataatt gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    26221 aaacaacatc ccgactgaga caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt
    26281 atcgtgggcg gcacggtgac tcaattcaga ctcgttcagt accatcccgg caaagaacgc
    26341 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    26401 cgccagcact gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat
    26461 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcaccttgcc
    26521 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    26581 tcctgccact gcgggcagca acaccagcgt cagaaccatc accaggtctt ccacaatcaa
    26641 ccaaccgatg gcgatttgcc cacgctgact gtctattaat tgccgttctt caagtgcgcg
    26701 cagtaacacc acggtactgg cggtggaaag acataagccg aacacgatac cggtcattaa
    26761 cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg ctatctgggc
    26821 gatcgcgccg ggaatggcga tggactttac cgccatcaaa tccttgagcg aaaaatgcaa
    26881 accgacgccg aacatcagca aaataacgcc cagttcagcc agttccggcg caagcttggt
    26941 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    27001 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    27061 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    27121 ttattgtcca tttttggctg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    27181 ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    27241 agcacggttc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    27301 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg
    27361 caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat agactaactc
    27421 gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc ccatgccgaa
    27481 agcaaaaccg aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc
    27541 gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac
    27601 ccccgtccag tgcaggcggg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat
    27661 agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt
    27721 acctgccgca acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa
    27781 tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata
    27841 tttcgagaaa atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag
    27901 tggattgatt atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca
    27961 acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac
    28021 gttgcctttg ccataaggct cgataatcac cgccgccagc aagggtccca gggaactgcc
    28081 aaagttgccg ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat
    28141 acgggctacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc
    28201 tgccagcaga actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt
    28261 aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac
    28321 cactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat
    28381 aaatgtcaga gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat
    28441 catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc
    28501 cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca ttgccatagg
    28561 aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac
    28621 taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc
    28681 tggatgcaga tttatcttca ccggacgcag acttgtctat gatgttgcgt catactattt
    28741 ttcaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc
    28801 gttagcgctg ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga
    28861 taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa
    28921 tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga
    28981 ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt
    29041 gcccgagtct gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta
    29101 tgacgcgatg gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca
    29161 ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga
    29221 gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg
    29281 gctgacaacc gatgacacag caaaaattgg taacctggaa tacttcactg atatcgaatt
    29341 tcgtaagccc gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc
    29401 agacattatt atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa
    29461 cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt
    29521 cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta
    29581 cgtgccgggt acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca
    29641 tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat
    29701 ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa
    29761 aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt
    29821 atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggca aaaccaatgg
    29881 tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat
    29941 tctggcagcc caaatggatc gcactggtgc cgactttgcg gtgatgagcg gaggcggaat
    30001 tcgtgattct atcgaagcag gcgatatcag ctataaaaac gtgctgaaag tgcagccatt
    30061 cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgattgatt acctgaccgc
    30121 cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt
    30181 ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa
    30241 aacttaccgt atggcgacat taaacttcaa tgccaccggc ggtgatggat atccgcgcct
    30301 tgataacaaa ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta
    30361 tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg
    30421 gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa tgcttaatcg cggcgggcga
    30481 tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt tcgatatcca
    30541 gtccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa
    30601 tcgttggcag acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg
    30661 aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta
    30721 cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat
    30781 caccgttcac tgccaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt
    30841 tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa
    30901 acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt
    30961 aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc
    31021 ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag
    31081 tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc
    31141 atttttttag cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac
    31201 cgcgaccaca tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg
    31261 ctgatgcatc agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt
    31321 attttgcggc ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt
    31381 atgccagtgg gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa
    31441 catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc
    31501 cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg
    31561 cgtcgcctga agaaccatcg cgatttgcca cagcggttgg gtagaaaaga gtgagggaga
    31621 aatgcccatc tcctggctaa tgtgctgcag gttttggagt tgctcctcgc taatgcgctc
    31681 ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc
    31741 ctcgacgatc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc
    31801 catatcgtgg ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag
    31861 ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt
    31921 tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg
    31981 agggatggcg caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc
    32041 ttgcatcgct attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac
    32101 cgacgacacc cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca
    32161 atgagttagc agagttgctg catgttcatc gtaatcgcgt cagtgcactg atcaataaca
    32221 atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg
    32281 attttaggct aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca
    32341 cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc
    32401 gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt
    32461 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    32521 gcctacaaac ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt
    32581 catgcgtcga tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    32641 cttcggttta aaccgcagca accggttggc gttacttact acggtaattg acgagagcgc
    32701 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    32761 ggcggcgacc ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat
    32821 gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg
    32881 catcagggta atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc
    32941 gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg
    33001 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc
    33061 aatcacttca tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc
    33121 ggtcaacatc accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
    33181 actacgcaac ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag
    33241 cacaggcgtt gctccttgcg atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc
    33301 gacttgctga tcatttaaca acgcctgatt acccagcaat aacgcatgac cttctgcttc
    33361 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc
    33421 gcctgcttta tcaaggatcg ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc
    33481 cgccagacgc agcgcctgcg cttcgtcaac atcagcaaat gttttcactg cgacaacctg
    33541 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    33601 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    33661 ggaaataatc gacatcggcg tcgccagtcc cagcgcacac gggcaggcga taatcagcac
    33721 cgtagtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    33781 tgccgcactg acaagcgcaa taaccactac taccggcaca aatatggctg agattttatc
    33841 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct
    33901 tgatagcgtg gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac
    33961 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggattg gttcgcccgt
    34021 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg
    34081 atcgccggtc gtcaggcgca gcaacatacc tggctgcact tctgccagag gcacgctttt
    34141 ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
    34201 tgccttagaa gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc
    34261 gataatcatc gcactggctt catagtaaag atgtcgcgct tccatcggga accactgcgg
    34321 ccacaggttg acgctcatcg aatagagcca cgccacgccc gttcccagcg ccaccagcgt
    34381 atccatcgtc gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgct
    34441 ggcgaaaacc atcaccgcca gggttatcag gccgataacc aaccacaggc tgcggttgtc
    34501 ggcggtgact atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccgc
    34561 cagtgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
    34621 gcgttcgcgg cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    34681 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    34741 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    34801 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    34861 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    34921 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    34981 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    35041 ttgttccaca tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca
    35101 ggacaggccg tccaggttca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    35161 tttgactggc tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa
    35221 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
    35281 catttatttc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    35341 gaaaatcggc actggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    35401 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    35461 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg
    35521 gcggcagtgg ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac
    35581 cgctttgcac tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc
    35641 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    35701 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
    35761 attgccacca ccagcctgat taacgctgaa aacgccgaac aacgctggca gcgaatttta
    35821 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    35881 gaacaaacaa ccaacttcca taatcgggcc attgcctggc tgctgtactc cgccggatat
    35941 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc
    36001 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    36061 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    36121 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
    36181 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    36241 ccaccgctgg acgaagaagg caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct
    36301 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    36361 gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg
    36421 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctggggcg
    36481 cctttgcttt tggcggtatt gtggctatgt tttccggtta tgcctatgcg cgtctggggg
    36541 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    36601 tcttttcgct agcgctctcg ttgctatacc tgttgacgct ggcggtgagc atcgccatgg
    36661 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    36721 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    36781 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    36841 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    36901 cccccagctc cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct
    36961 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    37021 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    37081 ttagcgatgt atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg
    37141 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    37201 cggcttcagg cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    37261 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    37321 atatcatcgt cgtggtgttg ataatgctga tgacggcggc gctaaattta gactcactcg
    37381 ccagcgttgc cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc
    37441 gcctgcgtca tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt
    37501 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    37561 ggattattgg tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa
    37621 ccgtataaca cctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc
    37681 gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc
    37741 gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc
    37801 attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat
    37861 cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg
    37921 ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac
    37981 agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag
    38041 gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac
    38101 agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga
    38161 ttaagacgag acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg
    38221 cgtacctgca ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
    38281 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
    38341 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
    38401 tttccaccac agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc
    38461 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
    38521 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
    38581 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg
    38641 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
    38701 ggcatcatca ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat
    38761 ttttgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc
    38821 attttggtgg cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac
    38881 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
    38941 aggatttccg cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc
    39001 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
    39061 tcaatacggg tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg
    39121 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg
    39181 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcatag
    39241 gcagcgcgtg gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg
    39301 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
    39361 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
    39421 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
    39481 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
    39541 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
    39601 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
    39661 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
    39721 tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
    39781 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
    39841 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
    39901 aaaccagaaa tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc
    39961 gatacggtat acgataatct gatctttccc tggcagatcc gtgaccagca gcctgacccg
    40021 gccatttttc tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaagaat
    40081 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa cctgcaattt
    40141 atgccgaagg ttttattgct ggatgaaata accagtgcgc tggatgaaag taataaacat
    40201 aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
    40261 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
    40321 ggagaaatgc aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact
    40381 ggcattaatg ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga
    40441 gaaagatatt ctatggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt
    40501 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
    40561 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
    40621 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
    40681 aggggcgatt gaatttatcc cgatgcaggt gatccctgtc gccgggatga ttgccggtaa
    40741 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
    40801 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
    40861 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
    40921 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
    40981 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
    41041 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
    41101 gactcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
    41161 gttcatgtgc cgaacaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
    41221 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
    41281 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc
    41341 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
    41401 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt
    41461 cagcagttcg atttgcgcta ccagcccctg gtagcgggtg tcctgatcct gcaacggaat
    41521 ggttttcagc accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag
    41581 cagcaggccg atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag
    41641 ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
    41701 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
    41761 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
    41821 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
    41881 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
    41941 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
    42001 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
    42061 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
    42121 catcacgcgc ccaggcagca ggcgctcgag caccattact gcccaggtca ccagcgttac
    42181 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc
    42241 ttccggtccc aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg
    42301 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
    42361 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
    42421 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
    42481 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
    42541 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
    42601 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
    42661 cagggcgatc acctcgtctg ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa
    42721 cacgccggta aatcccatgt tgttcatacg ctcaacatca ttcggttttc ggcaacctgc
    42781 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
    42841 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
    42901 atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct
    42961 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
    43021 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
    43081 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
    43141 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
    43201 gctgtggctg aaaaccacgc aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg
    43261 ctgatgctgt ttgagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
    43321 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    43381 ggccgcgctg gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt
    43441 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    43501 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    43561 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    43621 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    43681 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    43741 aagttgcgcg cgaagcacgt cggctttgtt tttcagttat ttatgttaat tcctaccctt
    43801 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    43861 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    43921 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    43981 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    44041 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    44101 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    44161 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    44221 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    44281 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    44341 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    44401 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    44461 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcggtctg caaactaatc
    44521 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    44581 ttaacctgaa aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag
    44641 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    44701 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    44761 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    44821 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    44881 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    44941 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    45001 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    45061 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    45121 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    45181 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    45241 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    45301 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    45361 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    45421 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
    45481 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    45541 agctttcggc attttcgctc tcctttatgc tgctggcact gctgctggtg ctgcgtggcg
    45601 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta
    45661 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45721 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaatgat aagccgacag
    45781 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45841 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45901 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    45961 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46021 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46081 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46141 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46201 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46261 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46321 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgatgtt cgccatgctt
    46381 gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa cggcactggc agtgttgcag
    46441 gcgaaagtgt ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc
    46501 agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt
    46561 aaggcgctgt tcaggcagtt tgtggggtga tgacaggtgt tagattgcct gatgcgctac
    46621 gcttatcagg cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga
    46681 taaggcgttt acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc
    46741 aactgtcatg tcggtttttt taaaggccga aggaggtaaa ccttcggcct tagctacgtt
    46801 tcctcagtta ttcaataaac tgacggatca gttccagcag cgcgattagc gccttaagaa
    46861 cgatgagaac tttatcgaga aaagttttca tccgttgctc ccttaaggga gaaagacaat
    46921 cacagcttac ccttacgttg cctataacta tgattttctt catgtgctag atgaggactg
    46981 tctctctcca aattagagca ccagaatcag caccacctgt atcctgacca ggacttaaaa
    47041 gtacttttcc gcactgcggt aatcccttga ggcagtatca ctactagcgt cgggattgta
    47101 tcgccaacat agctgtttga atatgtaggc atcctgactt ataattcact tgctagatga
    47161 ggactgttta atttctgccc ggaaccacca agtcttgggc ggaaaaaata aaaaaaccga
    47221 ctgtcatgtc ggttttttta tatctgtatt ctggtgcaac cattttgttc tcacgacaat
    47281 cgccgtttca gaataccgcc catcactgcg ccttaaccca taccgccact tccgcccact
    47341 caccgcgaaa gacaactttt tccgcttttt tctcaagctg atagcgatac attgggtcgt
    47401 aatattcttt aagtaacggc accagccagg ccagatgacc gtcggtgctg ccggtggtga
    47461 gttgcgttgt cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca
    47521 gccgacgctt aatcgccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct
    47581 gttcgtcgcc ataagcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcattca
    47641 ggcgttcaag acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc
    47701 gctcgcgcag acattccggc aggtgattcg aaccgatcat ccggctttca tcttccagca
    47761 cccacaggcg caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt
    47821 caaaactcgc ctggctaagt tgtggttgta acgtgcgacc aaacgccgaa ccgcgatgac
    47881 gcgccagccc ttccagatca acaccgtttg gctgttgttg cactaatagc gttttaccgc
    47941 tgccggtaca accgccaatc agcactatcg gtttttgtgc cagttcaatg gtcgcctgaa
    48001 tcgcggtctg gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctt
    48061 catgcagcca gcgttgcaca atatgtgaac gttgaccgcc acgggcgcag cagagaatac
    48121 cttgcggatt ttgcaggcac gctgcccgcc aggcgtacat acgctgctga cgaatttcac
    48181 ccgccaccag tttatgcccc agcgccagcg ctgcgtctga gccttgctgt ttatagcagg
    48241 tgccaacgga ggcgcgttca tcgttatcca ttaacggcag attgatagcg gcgggcattg
    48301 cgccttgctc aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca
    48361 gggcacgata gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca
    48421 gcttgcgcgc tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtggggt
    48481 gccgaaaatt tctaaaaatc cgctgatttc cggcctgtgt tgggtaaaca aggtcacaat
    48541 atcttctacc gctttgcagc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg
    48601 gcgcatcgtt gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag
    48661 tgattttggc aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat
    48721 atcgggaaca ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt
    48781 gccggcgctg tcttcaatat tgaccgccgg aaagcgacgc aactgcgctt ctgttagcgg
    48841 ctcttcaacg ttcgccagcg gatgatccgc cgccatgaca aatcgccatt gcaccgatcc
    48901 taagggatca agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag
    48961 cgaaaaacct tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg
    49021 aaactgggta aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg
    49081 gttatagagc aggttgttga tgacaatatt cacctggcgt tccacgccat cattcacctg
    49141 ttgcagctcg ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag
    49201 atgctcgcca gccgctgtca acgttacgct acgagtcgta cggaaaaaca gcgctactcc
    49261 ggtattctct tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg
    49321 ttctgccgct tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc
    49381 tggatcgaac atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc
    49441 tctgatttat cagtattttt acatgataat cctgttcaat ttgtggacta aatctagttt
    49501 tggaaaaaca ttccaacttt tgtattaatg ttgttctctt aaggttttag attgcctgtt
    49561 attgaaacca agctgaccgg tcggcggtgg ttgaacggaa ttatgttaca aggacaaaaa
    49621 gatgaaactt caggtattac cgttaagtca ggaagccttt agtgcttatg gcgacgtaat
    49681 cgaaacgcag caacgggatt ttttccatat taacaatggc ctggtggagc gttaccacga
    49741 tttggcgctg gttgagattc ttgagcaaga ccgtacgctt atcagcatta accgcgcgca
    49801 accggcgaat ctgccgctga ccattcacga actcgaacgt catccgctgg gcattcaggc
    49861 ctttatccca atgaaaggtg aagtgtttgt ggtggtcgtg gcgttaggcg acgacaaacc
    49921 agacctgtca acgctgcggg cgtttatcac caacggtgaa cagggggtga attaccatcg
    49981 taacgtctgg catcacccac ttttcgcctg gcagcgcgtc accgattttc tgaccatcga
    50041 tcgcggcggc agtgacaact gtgatgttga aagtattcct gaacaggaac tctgttttgc
    50101 gtgacgcctg caaccgactt gcataagata aactaattgt tcattgttta tgctcacttg
    50161 taggtcggag ttaacgtagg tatgacggaa gttagacggc gcggcaggcc aggacaggcg
    50221 gagcctgtgg cacagaaggg cgcacaggcg ttagagcggg gaattgcgat tctgcaatat
    50281 ctggaaaaaa gtgggggaag ttcgtcggtt agcgatattt ctctcaatct ggatttgccg
    50341 ctctccacga cctttcgctt gctgaaggtt ttacaagcag cggattttgt ctatcaggac
    50401 agtcaattag gctggtggca tataggatta ggtgtcttta acgtcggtgc ggcgtacatc
    50461 cataaccgcg atgtcctctc cgtcgccggg ccgtttatgc gccgcctgat gttactttcc
    50521 ggcgaaacgg tcaatgtcgc gatccgtaac ggcaatgaag cggtattaat tggtcagtta
    50581 gagtgtaaat cgatggtcag gatgtgtgcg ccactgggca gtcgtctgcc gctgcatgct
    50641 tccggtgcgg gcaaagcgct gctttatccg ctggcggaag aggagttgat gagcatcatt
    50701 ctgcaaaccg gtttgcagca gtttacgcca actacgcttg tggatatgcc caccttgctg
    50761 aaggacctgg aacaagcgcg tgaactgggc tataccgtag ataaagaaga gcatgttgta
    50821 ggtctgaatt gcatagcttc agcaatttac gatgatgtcg gtagagttgt tgccgctatc
    50881 tccatctccg ggccttcatc aagactgaca gaagatcgtt ttgtcagtca gggtgagctg
    50941 gtcagagaca ccgcccgcga tatcagcacg gcgttgggac tgaaagcaca tccatagtgt
    51001 ctgtcgcatc ccgctctgcg gagcgggttt ttttgacaaa atttgaaagt tggaaaattt
    51061 ttccaactaa tagaggtagg aataaaatgg caaaaatgag agccgttgac gcggcaatgt
    51121 atgtgctgga gaaagaaggt atcaccaccg ccttcggcgt tccgggagct gcaattaatc
    51181 cgttctactc agcgatgcgt aagcacggcg gtattcgtca cattctggcg cgtcatgtgg
    51241 aaggtgtttc gcacatggcg gaaggttata cccgcgcaac ggcagggaat atcggcgtat
    51301 gtctggggac ttccggtcct gcgggcacgg acatgatcac cgcgctctat tccgcttctg
    51361 ctgattccat tcctattctg tgcattaccg gccaggcacc gcgcgcccgt ctgcataaag
    51421 aagattttca ggccgtagat attgaagcaa ttgctaaacc ggtcagcaaa atggcggtta
    51481 cagttcgtga agcggcgctg gtgcctcgcg tgctgcaaca ggcatttcac ctgatgcgtt
    51541 ctggtcgtcc gggtccggta ctggtggatt taccgttcga cgttcaggtt gcggaaatcg
    51601 agtttgatcc tgacatgtac gaaccgctgc cggtctacaa acctgctgcc agccgtatgc
    51661 agatcgaaaa agctgtagaa atgttaatcc aggccgaacg tccggtgatt gttgccgggg
    51721 gcggggtaat caatgctgac gcagctgcac tgttacaaca gtttgctgaa ctgaccagcg
    51781 ttccggtgat cccaacgctg atgggctggg gctgtatccc ggacgatcat gaactgatgg
    51841 ccgggatggt cggtctgcaa accgcgcatc gttacggtaa cgcaacgttg ctggcgtccg
    51901 acatggtgtt tggtatcggt aaccgttttg ctaaccgtca taccggttcg gtagagaaat
    51961 acaccgaagg gcgcaaaatc gttcatattg atattgagcc gacgcaaatt ggtcgcgtgc
    52021 tgtgtccgga tctcggcatt gtctctgatg ctaaagcggc gctgacactg ctggttgaag
    52081 tggcgcagga gatgcaaaaa gcgggtcgtc tgccgtgtcg taaagaatgg gtcgccgact
    52141 gccagcagcg taaacgcact ttgctgcgca aaacccactt cgacaacgtg ccggtgaaac
    52201 cgcagcgcgt gtatgaagag atgaacaaag cctttggtcg cgatgtttgt tatgtcacca
    52261 ctattggtct gtcacaaatc gctgcggcac aaatgctgca tgtctttaaa gaccgccact
    52321 ggatcaactg tggtcaggct ggtccgttag gctggacgat tccggcggcg ctggggggtt
    52381 gtgccgctga tccgaaacgc aatgtggtgg cgatttctgg cgactttgac ttccagttcc
    52441 tgattgaaga gttagccgtt ggcgcgcagt tcaacattcc gtacatccat gtgctggtga
    52501 ataacgccta tctcggcctg attcgtcagt cacaacgcgc ttttgacatg gactactgcg
    52561 tgcaactcgc tttcgagaat atcaactcca gtgaagtgaa tggctacggt gttgaccacg
    52621 taaaagtagc ggaaggttta ggttgtaaag ctattcgggt cttcaaaccg gaagatattg
    52681 cgccagcctt tgaacaggcg aaagccttaa tggcgcaata tcgggtaccg gtagtcgtgg
    52741 aagttattct cgagcgtgtg accaatattt cgatgggcag cgaactggat aacgtcatgg
    52801 aatttgaaga tatcgccgat aacgcagcgg atgcaccgac tgaaacctgc ttcatgcact
    52861 atgaataagg gagataaatg atgttacgtt tctctgctaa tttatcgatg ttatttggtg
    52921 aatatgattt tctcgcccgt tttgagaaag ctgcgcagtg tggttttcgc ggcgttgaat
    52981 ttatgtttcc ttatgactac gacattgaag aattaaaaca ggtactggcg agtaataaac
    53041 tcgaacatac gctgcacaat ttaccggcag gtgactgggc ggcgggcgag cgcggtattg
    53101 cctgtattcc tggccgtgaa gaagagtttc gggatggcgt agcagcagcg attc
//
LOCUS       DAJTRM010000017        52261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRM010000017 DAJTRM010000000
VERSION     DAJTRM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.017"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(56..955)
                     /gene="nhaR"
                     /locus_tag="OO322_001081"
     CDS             complement(56..955)
                     /gene="nhaR"
                     /locus_tag="OO322_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5906043.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1021..2187)
                     /gene="nhaA"
                     /locus_tag="OO322_001082"
     CDS             complement(1021..2187)
                     /gene="nhaA"
                     /locus_tag="OO322_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5906044.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2773..2925
                     /gene="mokC"
                     /locus_tag="OO322_001083"
     CDS             2773..2925
                     /gene="mokC"
                     /locus_tag="OO322_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5906045.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3029..4159)
                     /gene="dnaJ"
                     /locus_tag="OO322_001084"
     CDS             complement(3029..4159)
                     /gene="dnaJ"
                     /locus_tag="OO322_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5906046.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4248..6164)
                     /gene="dnaK"
                     /locus_tag="OO322_001085"
     CDS             complement(4248..6164)
                     /gene="dnaK"
                     /locus_tag="OO322_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5906047.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6541..6945
                     /locus_tag="OO322_001086"
     CDS             6541..6945
                     /locus_tag="OO322_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5906048.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6971..7684
                     /gene="msyB"
                     /locus_tag="OO322_001087"
     CDS             6971..7684
                     /gene="msyB"
                     /locus_tag="OO322_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5906049.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            7833..8399
                     /gene="satP"
                     /locus_tag="OO322_001088"
     CDS             7833..8399
                     /gene="satP"
                     /locus_tag="OO322_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5906050.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8434..9021)
                     /gene="mog"
                     /locus_tag="OO322_001089"
     CDS             complement(8434..9021)
                     /gene="mog"
                     /locus_tag="OO322_001089"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5906051.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9136..10089)
                     /gene="tal"
                     /locus_tag="OO322_001090"
     CDS             complement(9136..10089)
                     /gene="tal"
                     /locus_tag="OO322_001090"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5906052.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10368..11798
                     /locus_tag="OO322_001091"
                     /pseudo
     CDS             10368..11798
                     /locus_tag="OO322_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            11868..12644
                     /gene="yaaA"
                     /locus_tag="OO322_001092"
     CDS             11868..12644
                     /gene="yaaA"
                     /locus_tag="OO322_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5906053.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(12797..13093)
                     /locus_tag="OO322_001093"
     CDS             complement(12797..13093)
                     /locus_tag="OO322_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5906054.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13307..14593)
                     /gene="thrC"
                     /locus_tag="OO322_001094"
     CDS             complement(13307..14593)
                     /gene="thrC"
                     /locus_tag="OO322_001094"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5906055.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14594..15526)
                     /gene="thrB"
                     /locus_tag="OO322_001095"
     CDS             complement(14594..15526)
                     /gene="thrB"
                     /locus_tag="OO322_001095"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5906056.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15528..17990)
                     /gene="thrA"
                     /locus_tag="OO322_001096"
     CDS             complement(15528..17990)
                     /gene="thrA"
                     /locus_tag="OO322_001096"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5906057.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18016..18135)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18071..18136)
                     /gene="thrL"
                     /locus_tag="OO322_001097"
     CDS             complement(18071..18136)
                     /gene="thrL"
                     /locus_tag="OO322_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5906058.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18350..19036)
                     /locus_tag="OO322_001098"
     CDS             complement(18350..19036)
                     /locus_tag="OO322_001098"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5906059.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19436..19576)
                     /locus_tag="OO322_001099"
     CDS             complement(19436..19576)
                     /locus_tag="OO322_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906060.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19672..20388
                     /gene="arcA"
                     /locus_tag="OO322_001100"
     CDS             19672..20388
                     /gene="arcA"
                     /locus_tag="OO322_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5906061.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20447..21799)
                     /gene="creD"
                     /locus_tag="OO322_001101"
     CDS             complement(20447..21799)
                     /gene="creD"
                     /locus_tag="OO322_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5906062.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(21857..23281)
                     /gene="creC"
                     /locus_tag="OO322_001102"
     CDS             complement(21857..23281)
                     /gene="creC"
                     /locus_tag="OO322_001102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5906063.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23281..23970)
                     /gene="creB"
                     /locus_tag="OO322_001103"
     CDS             complement(23281..23970)
                     /gene="creB"
                     /locus_tag="OO322_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5906064.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(23983..24456)
                     /gene="creA"
                     /locus_tag="OO322_001104"
     CDS             complement(23983..24456)
                     /gene="creA"
                     /locus_tag="OO322_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5906065.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24667..25536
                     /gene="robA"
                     /locus_tag="OO322_001105"
     CDS             24667..25536
                     /gene="robA"
                     /locus_tag="OO322_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5906066.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25533..26180)
                     /gene="gpmB"
                     /locus_tag="OO322_001106"
     CDS             complement(25533..26180)
                     /gene="gpmB"
                     /locus_tag="OO322_001106"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5906067.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26232..26752
                     /gene="yjjX"
                     /locus_tag="OO322_001107"
                     /pseudo
     CDS             26232..26752
                     /gene="yjjX"
                     /locus_tag="OO322_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(26837..27163)
                     /gene="trpR"
                     /locus_tag="OO322_001108"
     CDS             complement(26837..27163)
                     /gene="trpR"
                     /locus_tag="OO322_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5906068.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27253..29190)
                     /gene="sltY"
                     /locus_tag="OO322_001109"
     CDS             complement(27253..29190)
                     /gene="sltY"
                     /locus_tag="OO322_001109"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5906069.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29401..31068
                     /gene="ettA"
                     /locus_tag="OO322_001110"
     CDS             29401..31068
                     /gene="ettA"
                     /locus_tag="OO322_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5906070.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31175..31342)
                     /locus_tag="OO322_001111"
     CDS             complement(31175..31342)
                     /locus_tag="OO322_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906071.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31376..32608)
                     /gene="nadR"
                     /locus_tag="OO322_001112"
     CDS             complement(31376..32608)
                     /gene="nadR"
                     /locus_tag="OO322_001112"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5906072.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32629..34011)
                     /gene="radA"
                     /locus_tag="OO322_001113"
     CDS             complement(32629..34011)
                     /gene="radA"
                     /locus_tag="OO322_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5906073.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34060..35028)
                     /gene="serB"
                     /locus_tag="OO322_001114"
     CDS             complement(34060..35028)
                     /gene="serB"
                     /locus_tag="OO322_001114"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5906074.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35134..35778
                     /locus_tag="OO322_001115"
     CDS             35134..35778
                     /locus_tag="OO322_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5906075.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            35806..36821
                     /gene="lplA"
                     /locus_tag="OO322_001116"
                     /pseudo
     CDS             35806..36821
                     /gene="lplA"
                     /locus_tag="OO322_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            36853..37116
                     /locus_tag="OO322_001117"
     CDS             36853..37116
                     /locus_tag="OO322_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5906076.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37277..37996)
                     /gene="deoD"
                     /locus_tag="OO322_001118"
     CDS             complement(37277..37996)
                     /gene="deoD"
                     /locus_tag="OO322_001118"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5906077.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38053..39276)
                     /gene="deoB"
                     /locus_tag="OO322_001119"
     CDS             complement(38053..39276)
                     /gene="deoB"
                     /locus_tag="OO322_001119"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5906078.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39328..40650)
                     /gene="deoA"
                     /locus_tag="OO322_001120"
     CDS             complement(39328..40650)
                     /gene="deoA"
                     /locus_tag="OO322_001120"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5906079.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(40777..41556)
                     /gene="deoC"
                     /locus_tag="OO322_001121"
     CDS             complement(40777..41556)
                     /gene="deoC"
                     /locus_tag="OO322_001121"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5906080.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            41814..43355
                     /locus_tag="OO322_001122"
     CDS             41814..43355
                     /locus_tag="OO322_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5906081.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43327..44190
                     /locus_tag="OO322_001123"
     CDS             43327..44190
                     /locus_tag="OO322_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5906082.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44404..45186)
                     /locus_tag="OO322_001124"
     CDS             complement(44404..45186)
                     /locus_tag="OO322_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5906083.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45183..46256)
                     /locus_tag="OO322_001125"
     CDS             complement(45183..46256)
                     /locus_tag="OO322_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5906084.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46378..46539)
                     /locus_tag="OO322_001126"
     CDS             complement(46378..46539)
                     /locus_tag="OO322_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5906085.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46656..47261)
                     /gene="osmY"
                     /locus_tag="OO322_001127"
     CDS             complement(46656..47261)
                     /gene="osmY"
                     /locus_tag="OO322_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5906086.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47654..49243)
                     /gene="prfC"
                     /locus_tag="OO322_001128"
     CDS             complement(47654..49243)
                     /gene="prfC"
                     /locus_tag="OO322_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5906087.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49334..50011)
                     /gene="yjjG"
                     /locus_tag="OO322_001129"
     CDS             complement(49334..50011)
                     /gene="yjjG"
                     /locus_tag="OO322_001129"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5906088.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50026..50472)
                     /gene="rimI"
                     /locus_tag="OO322_001130"
     CDS             complement(50026..50472)
                     /gene="rimI"
                     /locus_tag="OO322_001130"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5906089.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50441..50854)
                     /gene="holD"
                     /locus_tag="OO322_001131"
     CDS             complement(50441..50854)
                     /gene="holD"
                     /locus_tag="OO322_001131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5906090.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            50957..51988
                     /gene="rsmC"
                     /locus_tag="OO322_001132"
     CDS             50957..51988
                     /gene="rsmC"
                     /locus_tag="OO322_001132"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5906091.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12414 a        14170 c        13108 g        12569 t
ORIGIN      
        1 gtggtaatgt acaactaatc tatctccccc accttaactt tgggggagct gccgattaac
       61 gcaccgctgg actaaaaagc gcagaataat ccgtattgca gattcgctgt accgccgggt
      121 gctgaatcat ccgctcagca aaaatagcat ggtactcttc catcacattc tcgacgcgac
      181 caatttctac gacagtttta tcggcataaa agtcatatgc ataaagcgtt ggggcaacga
      241 agattgcatt gtgcatcgca ccaaaagctt tcatcaaagc ggcatcatca aactcgccga
      301 ggatttctac gtttaatccc tgggagttaa accagttaag caatttgcgc cctaacattg
      361 aacgtcgccc aggaatcaaa agtcgccgtt cttccagaca agccgggaac ggtttttctg
      421 gtggtggatt tgtacaccag aaactcacgc cacattcgcc aattctcacg gagaacaggc
      481 cttcctgctg cgtagagtct atcggacagt cagaaatgat catatccagt ttatgctgac
      541 ttaattgctc cagcagcatt tcgtgggtgg attcgaagca gcgaagatga atgggctcgc
      601 cttctaccac tgcggcgtta agtacgctac tgaccaggcg tttggaaagt gcatcagcca
      661 cgccaacgtc aaacaataaa ttggattctt tgcgatagtt cacaatatcc agcatttcct
      721 ggcttaaggt gaacatttta tcggcatagc gatagaccag ttctcccagc tcgctgggtt
      781 cgagaccacg tcccttgcgt ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa
      841 tctgtccggt aatggtttgt ggagttaaat aaagcgcctc cgctgcgcca accacggaac
      901 cttctttata gacatgccag aagtaataca agtggttgta attgatatga gacatgctca
      961 tttctctccc tgataacaat gaaaagggag ccgtttatgg ctccccggta aaccgtcctg
     1021 tcaaactgat ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat
     1081 agaaccgacc aggataccga gtttcgccca gttaatcagt tctggatcta cgctaccaaa
     1141 ggccaggctg gcaataaaga tagacatagt aaaaccgata ccgcacagga tccccaccac
     1201 cataatttgc tgataagtcg ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa
     1261 ccagcagaac agactaatcc ccagcggttt gccaatcagc aagccagcga tgatccccaa
     1321 tggcagaatg gaggtcaagc catccagcgt gacgccttgc agtgaaacgc cagcattagc
     1381 aaatgcaaac agcggcaaaa tcagatacgc cacccacggg tgcaacacat gctccagtcg
     1441 cttcgctgga gaacgcccat gcttctcttt caaaggaata aagaagccga caattacccc
     1501 cgccagagtt gcgtgaaccc ccgatttcaa caccgcagtc cacaacacca cgccaacaag
     1561 aatatagacg cccgtgcggc gtacaccaca cagattcaat accgcgagta ccgcaattgc
     1621 tacagccgcg acgccaaggg aggccatcga taagtcatta gtgtagaaca atgcgatgat
     1681 aatgatggcc ccaagatcgt cgataatagc cagagccatc aaaaagatct tcagcactaa
     1741 cggaacacga cttcccaaca gcgccagtac accaagtgca aaagcaatgt cagtagccgc
     1801 cgggatcgcc cacccttcgc gggtaatcgg atcggcatag ttaaaagcca gatagagtaa
     1861 tgccggcaca atcatcccac caatagcggc gataactgga aatgcggcct ggcgtaagct
     1921 ggctagcgat ccttgcatca gttcacgttt aacttccaga ccgactaaca ggaaaaatac
     1981 cgccatcagc gcatcattta tccataacag catgtttttg ttgatttcga gtgaaccaac
     2041 ccggagctga accggcgtct ccagaaagtc gtgataccat ccactggttg cgccgctgtt
     2101 ggccataatc atcgccagga tagcggcaat gataagaata atgcctcccg aggcatcact
     2161 gctaaagaat cgatgcagat gtttcacttt ttatttctct ttcaggtgaa tagatcgttt
     2221 ttactatttt accccgcacg aatcatcgtt aaaatcagat tatagtgtag gaataattcg
     2281 ttttttacga gccattgaag aatttgcgcc atcagcggtg agatgcgttt ttgacggctg
     2341 taagcgctca cgagtgaccc taagcccaga taatcatagc ttaaggtgag gattcctgag
     2401 ggattaatta ggaaaagtaa taattaattt gttgtcagtc ggcgatgctt gttaggccaa
     2461 tatttcaaaa ataatccagc ttgtcgtcaa taattgcgat gaagagataa attaaagtta
     2521 aataattaaa tcattaaaaa attacgccac aaaaatgcga ggcgtcttca ggattagaat
     2581 cgataaaaca aaatgggtta aagagagcga tataatagcg gcgggtgctt gaggctgtct
     2641 gtctcaggca ttagctgaac ggcagataga gaaaagcccc gagtgatatt ttaccatcaa
     2701 cccgaggcct cctatatgct gaacacatgt agagtgcctc ttactgaccg taaggtcaag
     2761 gagaagagag caatgaagca gcataaggcg atgattgtcg ccctgatcgt catctgtatc
     2821 accgccgtag tggcggcgct ggtaacgaga aaagacctct gtgaggttca catccgaact
     2881 ggccagacgg aggttgctgt tttcacggct tacgaatccg agtaagagca acggcgggga
     2941 gtgatccccg ccactttaca ggtgctcgca tatcttcaac gcaccctatt tttacccagg
     3001 cctgcccacg ggcaggcttt tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca
     3061 caccatcaaa gaagctcttt gagcgcgggc tgttgtgctc gccggttggg ccaccgaagc
     3121 tttcttgcag ctcttgcagc agctgtttct gcttttcgtt caggcctacc ggtgtttcga
     3181 cgacaacgcg gcacagcaaa tcaccctgtg cgccaccgcg gacagacttg acgcctttac
     3241 cgcgcatacg gaatagctta ccggtctggg tttcgccagg cactttcagt ttgacgcgac
     3301 catcaagggt cggtacttcg atttcgccac ccagcgccgc catagcgaag ttgatcggga
     3361 cttcgcaata caggttgttg ccttcacgct cgaaaatcgg gtgctgttta acctgaacct
     3421 gaacgtacag atcgcctgcc ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac
     3481 ggatgcggtc tccagtgtcc acccctgccg ggattttaac ggacagcgtt ttgctgcgct
     3541 caacacgacc atgaccatga catttgttgc acggatcttt gatcagcgta ccgcggccct
     3601 gacagtgtgg acaggtctgc tgtacagcga agaatccctg gcgcatctgc acctgaccag
     3661 aaccatgaca ggtcggacag gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc
     3721 aaacgtcaca ctcttccaga gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt
     3781 cttcgagggt gagctccatg ttatagcgta aatcagcacc gcgcgccgca cgttgacgac
     3841 cacgtccgcc gccaaaaata tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc
     3901 cgccgccaaa accaccgccg cccatgcctc cttgctcaaa cgcagcatga ccatactgat
     3961 cgtatgccgc acgtttttgc gagtcggtca gaacttcata agcttccttg atctctttaa
     4021 atttcgcctc ggcctctttg tcaccctggt tacggtccgg gtggtatttc atggccaggc
     4081 gtttgtaggc ctttctgatt tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt
     4141 aataatcttg cttagccatc ttttttaaat tgcccctaga tgaatgcacg ggcggagagg
     4201 aaattcccct tcgcccgtgt cagtataatt acccgtttat agggcgatta ttttttgtct
     4261 ttgacttctt caaattcagc gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca
     4321 tcagcaccgg cagtctgctg ctgggcatgt tgctgctggg cgatttccat cagtttctgg
     4381 gaaacctgtg ccagttcctg cattttcgct tcgatagcgg ctttgtcttc acctttcaga
     4441 gcagtttcca gtgcagtcag cgcagactcg atagcagttt tgtcgtcagc cggcagtttg
     4501 tcgcctgctt cttcaacctg cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga
     4561 gtctgtacca gctcttcaaa cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc
     4621 attttctgga tttcatcttc gttcagacca gaagaagcct tgatggtgat cttctgctct
     4681 ttaccgctgt ttttatcttt cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag
     4741 gtaacttcga tctgcggcat gccgcgcggt gccgggttga taccatctag gttgaactga
     4801 cccagagatt tgttatcagc cgcacgttta cgttcaccct gcagcacatg gatggttacc
     4861 gcagactggt tgtcttcagc ggtagagaac acctggctgt gcttggtcgg gatagtggtg
     4921 tttttcgcga tcagcgtcgt catcacaccg cccatggttt cgatacccag agacagcggg
     4981 gtaacgtcca gcagcagtac gtcttttacg tcaccagtca gaacaccacc ctgaacagca
     5041 gcaccgattg ctacagcttc gtccgggtta acgtctttac gcggctcttt accaaagaac
     5101 tcagcaactt tcttctgaac cattggcata cgagtctgac caccaacgag gataacgtcg
     5161 tcgatatcag atacggacag gccagcatcc tgcagtgcaa ctttcagcgg ctcaatggaa
     5221 cggtttacca gatcttcaac caggctttcc agtttcgcac gagtcacttt gatgttcatg
     5281 tgtttcggac cggtcgcgtc tgcagtgatg tatggcaggt taacgtcggt ctgctgagcg
     5341 gaagacagtt cgattttcgc tttttctgcc gcttctttca ggcgctgcat tgccagcgga
     5401 tcgttgcgca ggtcaatgcc ctgatctttc ttgaattctt caaccagata gttgatcaga
     5461 cggctgtcga agtcttcacc acccaggtgg gtatcaccgt tggttgccag aacttcgaag
     5521 gttttttcgc cgtcaacttc gtcgatttcg ataatagaaa tatcgaaagt accacccccc
     5581 aggtcataaa ccgcgatagt acggttgcca gtgcctttgt ccagaccgta agccagcgca
     5641 gctgcggtcg gttcgttgat gatacgtttt acttccagac cagcgatacg gcctgcgtct
     5701 ttggttgcct gacgctgagc atcgttaaag tatgccggta cggtgataac agcttcagtt
     5761 accggttcac ccaggtaatc ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa
     5821 atctgcggcg gtgccatttt ctggccttta acttcgaccc atgcgtcgcc gttatcagca
     5881 gcaataattt tgaacggcat gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg
     5941 cggccaatca ggcgtttaat cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt
     6001 ttagccggct gaccaaccag agtttcacca tcctgggtat aggcaatgat agaaggcgtg
     6061 gtgcgatcgc cttcggcgtt ctccagcacg cgaggaatgg tgccatccat aatcgctaca
     6121 caagagttgg tagtacccag gtcgatacca attattttac ccatctaaac gtctccacta
     6181 tatattcggt catcatgtgg ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact
     6241 gcccaatttc atttttttgc agactcactc actgcggttg actactaaat ggggtcgtaa
     6301 cccacgtcat caagggggag atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga
     6361 tatggcagga caagacgtaa aattgacagc aattttattc atataaagaa tgaatcgcag
     6421 ccaaggcgtt catcaaaaaa ttgtaataaa aagaaaagat tacgtgcctg aatcttctct
     6481 ttatcagcag taaactagtg ggtattcatc cccctacctc ttcccactaa gagaatcctt
     6541 atgaaatccg tttttacgat ttccgccagc ctggcgatta gcctgatgct gtgctgcacg
     6601 gcgcaggcaa acgaccataa actcctcggc gtcattgcaa tgccgcgtaa cgaaaccaac
     6661 gatctggcgc tgaaacttcc tgtttgtcgc attgtgaaac gcatacaact ctctgccgac
     6721 catggcgatt tacagttaag tggtgcatcg gtttatttca aagccgcccg tagcgccagt
     6781 cagagcctga atattccttc agaaataaaa gaagggcaaa ccactgactg gatcaacatt
     6841 aacagcgata acgacaataa acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg
     6901 aactcatcgg atatggccac gctgaaaatt atcggcgacg actaacgcca gaatatagaa
     6961 gccacaaaaa atgaatgtta attacctgaa tgattcagat ctggattttc ttcagcattg
     7021 tagtgaggaa cagttggcaa atttcgcccg attgctcacc cataatgaaa aaggcaaaac
     7081 tcgcctctcc agcatactga tgcgcaacga actgtttaaa tcgatggaag ggcatcccga
     7141 gcaacatcgc cgcaactggc agctgattgc cggagaatta cagcattttg gtggcgatag
     7201 tatcgccaac aaactgcgcg gacacggtaa attgtatcgg gccattttgc tcgatgtttc
     7261 aaagcgattg aagctgaaag ccgacaaaga gatgtctacg tttgaaattg agcaacagtt
     7321 actggaacaa tttctgcgta atacctggaa gaaaatggac gaggaacata agcaggagtt
     7381 tctgcacgcg gtcgatgcca gggtgaatga gctggaagag ctgctgccgc tgctgatgaa
     7441 agacaaatta ttggcaaaag gtgtgtcgca tttgctttcc agccaactga cccgcatttt
     7501 acgcacccac gcagcaatga gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg
     7561 aggccctgtg ggcgcagcat taaatggggt taaagcggtc agcggcagca cctatcgcgt
     7621 gacgattcca gccgtactgc aaatcgcctg cctgcgccgg atggttagcg ccactcaggt
     7681 ctgataccgg aaaccgggga aaattttcat tttcccctca aaagatcgta gacactgccc
     7741 cactggctga ttattatgcc gcgccctgaa aacactacag ttattcaggg aaattatttc
     7801 accattcatt cgatgatgat ttttgaggaa ttatgggcaa cactaagttg gctaatccgg
     7861 caccgctggg cctgatgggc ttcggcatga ccaccattct gcttaacctg cacaacgtgg
     7921 gttatttcgc tctggacggt attattcttg ccatgggcat tttctacggc ggcatcgcgc
     7981 aaatttttgc tggtctgctg gagtacaaaa aaggcaacac tttcggttta accgcattca
     8041 cctcttacgg ttctttctgg ctgacgctgg ttgcgattct gctgatgccg aaactgggtc
     8101 tgaccgatgc gccaaatgca cagttccttg gtgtctacct gggtctgtgg ggcgtattta
     8161 cgctgtttat gttcttcggc actctgaaag gcgcgcgcgt cctgcaattc gttttcttta
     8221 gcctgaccgt gctgtttgcc ctgctggcga tcggtaacat tgccggtaat gccgcgatca
     8281 tccactttgc cggctggatt ggtctgatct gcggtgccag cgcaatctat ctggcgatgg
     8341 gtgaagtgct gaacgagcag tttggtcgca ccgttctgcc gattggtgaa tcccactaat
     8401 tgtcttgaga tccccccgct cggggggatt tttttattcg ctaacgtcgc gtcttgcact
     8461 cttcggtctg aatgcagcaa ccacttctgg tgccgtttca acgtatggcc cttccagcaa
     8521 ctgaatgcag tacggtacgc tggcaaaaat accatgcacc acaacgttac cctcagcgtc
     8581 cttcacacct tccagcgtct ctttaataga cttcggctga ccgggtaagt taaggatcag
     8641 cgcctgtttg cgaatcacgc ccacctgacg cgaaaggatc gcagttggta caaaatgcag
     8701 gctgatctgg cgcatctgtt caccaaagcc aggcatctcg cggtccgcta ctgccagcgt
     8761 cgcatcgggc gttacgtcac gacgcgccgg gccagttccg cccgtggtga gcaccagatg
     8821 gcaactcatt tcatccacca gctcacacaa cgtttgctcg atgatcgcct gctcatcggg
     8881 gattaagcgg gtttccagtt caaacggcgt ggttagcgcc gatgtcagcc attcttccag
     8941 cgctggaatg cctttatcct gataaacgcc gctggatgcg cgatcagaga tggaaactaa
     9001 gccaatacgt aaagtattca tcaagatttt tccgtttaaa caatctcgtt aaacagaatg
     9061 atacactgcg aagggagtga cagacaggct tcagaagagg tagcgtgacc gacttcccgg
     9121 tcacgctaag aatgattaca gcagatcgcc gatcattttt tccagttttt cctggtcaat
     9181 agcaaactta cggatacctt ccgccagttt atctactgcc attggatcct ggttgtgctg
     9241 ccacaggaac tcggactcag tgatacgcgc cggacgcgct ttcacttcgc cagtgtaaga
     9301 cagtttacgt tcgatagccc cttcgctctc cgccagctct ttcagcagtg ccggtgcgat
     9361 ggtcagacgg tcacagcctg ccagttccag aatttcgccg atgttacgga agcttgcgcc
     9421 cataaccacg gtttcataac cgtgctcttt gtagtactgg tagatttcag atacagaaac
     9481 cacgcccgga tcttctgccg gagcgtactc tttcttatcg gtattcgctt tgtaccagtc
     9541 aagaatacgg ccaacaaacg gcgagatcag gaacacgccc gcctccgcac aagcacgagc
     9601 ctgagcgaag gagaacagca gggtcaggtt acagttgata ccttcttttt ccagctgttc
     9661 tgcagcacgg ataccctgcc aggtagaagc cagtttgatc agaatacgat cgttgctaat
     9721 acctgcatcg ttgtagagtt tgatcaggcg ttttgctttc gcaattgacg cttcagtgtc
     9781 ataggaaaga cgcgcatcaa cttcagttga gatacggccc ggaaccagtt tcaggatttc
     9841 cagaccaata tttactgcca gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt
     9901 gctctgctgt ttcgcccagg cgacagcatc atcaatcaac ttacggtatt ccggaatctg
     9961 cgctgcgtta agaatgagag aagggttggt tgtggcatcc tgcggttgat acagcttcat
    10021 tgccgcgatg tccccagtgt cggccactac ggtggtgtac tgacgaaggg aggtcaattt
    10081 gtccgtcatg atagtatttc tctttaaaca gcttgttagg gggatgtaac cggtctgccc
    10141 tgatgatatc acgacgcaac gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag
    10201 actgtttcgg atttctgaca ctgcgttgat atcgcccacc atttttatac aaaacctcat
    10261 gtatgctacg cagaagttat caagtacctc gtagcgtata tacttcttaa acaattggta
    10321 acgtttacac aggaaagtca tcgcgaccgg caataagagg gatatgcatg ccagattttt
    10381 tctcctttat taacagcgtc ctttggggat cggtaatgat ttacctgctc ttcggcgcag
    10441 gttgttggtt cacttttcgc accggatttg tgcagtttcg ctacatccgc cagtttggca
    10501 aaagtcttaa aaatagcatt catccacagc caggcggttt aacctcattt cagtcattgt
    10561 gtaccagtct tgcggcgcgc gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta
    10621 ccgccggtgg accaggtgcc gtcttttgga tgtgggttgc tgcgtttatc ggcatggcga
    10681 cctcgtttgc cgaatgttcc cttgcacaac tttataaaga acgtgacgtc aatgggcagt
    10741 ttcgtggcgg accggcatgg tatatggcgc gcgggctggg gatgcgctgg atgggcgttc
    10801 tgttcgccgt ctttttgctc atcgcctacg gcataatttt cagcggagtt caggcgaacg
    10861 ccgttgcccg agccctgagt ttttcttttg attttcctcc gctggtgacg ggcattattc
    10921 tcgctgtctt tgctctgctg gcaatcactc gtggtcttca tggcgtcgcc cggctcatgc
    10981 aggggtttgt cccgttgatg gcgataatct gggtactgac cagcctggtg atttgcgtaa
    11041 taaatatcgg gcaacttccc cacgtcattt ggtctatttt tgagagtgct tttggctggc
    11101 aggaagcggc aggcggcgcg gcgggatata ccttaagcca ggcgattact aacggttttc
    11161 agcgcagtat gttttccaat gaggtgggaa tgggatcgac gccaaacgcg gcagcggcag
    11221 ctgcatcctg gcctccgcat ccggcagcac aagggattgt ccagatgatt ggcattttta
    11281 tcgacaccct ggtcatctgt acggcaagcg ccatgctgat attactggcg ggtaacggca
    11341 caacctacat gccgctggaa ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg
    11401 gttcctaggg tgctgaattt gttaccctcg tggttattct gtttgccttc agctccatcg
    11461 ttgccaacta catttatgcc gaaaacaatc tcttcttttt acgcctgaac aaccctaaag
    11521 cgatctggtg tttgcggatc tgcaccttcg caacggtcat cggcggcacc ttgctaagcc
    11581 ttccgctgat gtggcaactg gcagatatca taatggcctg catggctatt accaatttga
    11641 ccgccatttt actgctctcg cctgtggttc ataccattgc cagtgattat ctacgccagc
    11701 gtaaactcgg cgtgcgcccg gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc
    11761 tttctccgga cgcgtgggat gatgtttcgc aggagtaatc acaactatcg atcaactcat
    11821 tctctttttt tgctaaagtc ggcataaatt tcctgcaagg actggatatg ctgattctta
    11881 tttcacctgc gaaaacgctt gattaccaaa gcccgttgac caccacgcgc tatacgctgc
    11941 cggagctgtt agacaattcc cagcagttga tccatgaagc gcggaaactg acgcctccgc
    12001 agattagcac gctgatgcgc atcagcgaca aactggcggg tatcaacgcc gctcgctttc
    12061 atgactggca gccagatttc acgccggcga atgcccgcca ggcgattctg gcgtttaaag
    12121 gtgatgtcta caccggcttg caggccgaaa ccttcagcga agacgatttc gattttgccc
    12181 aacagcattt gcgaatgctt tccggcttgt atggcgtact gcgcccgctc gatttaatgc
    12241 agccttatcg tctggaaatg gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc
    12301 aattctgggg agatatcatc accaacaagc tgaacgaggc gctcgcagca caaggcgata
    12361 atgtggtgat taatctggcg tcagatgagt attttaaatc ggtgaagccg aagaaattga
    12421 atgccgagat tatcaagccg gtgttcctcg atgagaagaa cggcaaattt aagatcatca
    12481 gcttctacgc taagaaagca cgcggtctga tgagtcgttt cattattgaa aatcggctga
    12541 ccaaaccaga gcaactgact ggttttaata gcgaaggtta cttctttgat gaagattcct
    12601 ccagcaatgg cgaactggtg tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc
    12661 ggatgcggcg tgaacgcctt atccggccta caaaagcatg cagattcaat atattgcaga
    12721 attaacgtag gcctgataag acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt
    12781 acccggcatt tgtcatttag cggtgatgtt tgcctggacc atgaccgccg tgatgatcat
    12841 gaggagcttt cttatggtgg cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc
    12901 ctcgccattc ataatgttgt ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt
    12961 aatagccacg attatcacga tcgcctatct gtaattttac tgacgggact aacgtaattt
    13021 ccgcagcctg tgctgccatg ggagcgacca gaaccaggga aagtgcgagt acgatagatt
    13081 gcatcttttt cacaggttac tccttttatc attgcccgtt atgggtcgat atccggagat
    13141 tacgcgatga gaacgacggt tgttattctc cgcaatccta atctctaagt taccgcattt
    13201 attgataatt tctccttttt ccctgtcgtt tttctccata atttcttcat aaaaaagccg
    13261 ggctgcataa aagcaaaccc ggcctgattg agataatgaa tagattttac tgatgattca
    13321 tcatcaattt acgcaacgca gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat
    13381 cagcacgttc tgccagctct tttggcagat ccaacgtttc accgagaatc gcttccacgc
    13441 tctctttaaa tttcgccgga tgcgcggtgc cgaggaacaa gccatattcg cctggattca
    13501 actggtcacg cagcgcacga tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt
    13561 cttttaactc acgcattgtc tgttgcgtgg tttcatcatc cacggctgca taacccagct
    13621 ctttcagttg ccagattttg cggcggaaca actcttccac acgcggccag ttgttcggct
    13681 gactcacgtc catcgcgttg gataacgtcg cctgagtcgc tttgggtgac cactgaccgt
    13741 cgtgcaggaa acgtggcacg gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg
    13801 gcagaccgaa tgacttcgcc agcagacccg ccgtcaaatc gccgaagttt ccgcttggca
    13861 ccgagacaac cagctggttg cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt
    13921 agcaaatctg cgccagcaaa cggctgatgt taatcgagtt agccgagttt aaccccagcg
    13981 ccactttcag ttcttcatca tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat
    14041 cgccgtcgat ggcaacagtt tcgatattgc cgcccaacgt acagaacagt ttttcttgca
    14101 gtggactgat tttgcctcgt ggatagagga taaccacttt cacattcggc aaaccgtaga
    14161 aagcatgagc cactgcagct ccggtatcac cggaggtcgc ggtcagaatg gtcactggct
    14221 tatcatccgc aatatgggtc agcatttgtg ccataaagcg accgccgaaa tctttaaatg
    14281 ccagcgttgg cccgtggaac aattccagac aaccgacatc gctttcaaca ttggcgaccg
    14341 gagccgggaa ggcaaacgcc gcgcgcacgc gctcttccag gatttcctgt gggatttcat
    14401 caccaataaa cgccgagagg atcttcgcac tgcgggtgac aaaatccagc ttcagcatct
    14461 catcaatttc agtcaggctg aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt
    14521 tgcccaaccc ctgggttacg gcttgcgcaa agctgacctg ctcgttgtga tctttcagat
    14581 tgtagagttt catttagttt tccagtactc gtgcgcccgc cgtatccagc cggcaaatat
    14641 gaacaaaacc ttcctgattt tgcaggtagt tcttacccaa ccagtcggca acgcgctggg
    14701 cggtatccgg cttgtcacat agagcgaaca aggtcgggcc ggagccggag ataccgctcg
    14761 ctaccgcgcc gatttccgcg accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt
    14821 cacggtaggg ttcagcgata acatctttca tcagcttcgc ggcaagctca ggctgacggg
    14881 aatagcaggc gtgaatgaag ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc
    14941 gatactgcgc cggtaaaata gcccgggctt ctgccgtcga gactttaatt cccggatacg
    15001 ccagcaccca cagccactca tcaaaccctg gcacttgctg gctgatgatg tcgttttctt
    15061 cgatcatcaa ctgcatacca ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc
    15121 cggagatacg tccttccagc tcgcccatca aagccagcaa acgagtgtca ttaagcggct
    15181 tgccgcagtg ttcattcatc gccatcagcg ccgcgaccac cgaacaggcg ctggagccta
    15241 agcccgaacc gatcggcata ttcttttcca gagtcatcgc cactggaatt tgcttgccaa
    15301 gctcctggca aaaacgctcc cagcactgat aaacgatatt ttcccgtggt tctgacggca
    15361 gcttatcggc aaagcgtccg agtttgttga gactgaatgt ctctgccgcc tcaaccgtga
    15421 ctacatctcc gagcaatgca ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc
    15481 cgacgctcat attggcactg gaagccgggg cataaacttt aaccatgtca gactcctaac
    15541 ttccatgaga gggtacgtag cagatcggca aagacaccgg cagcggtaac gtcattgccc
    15601 gcaccatatc cgcgcagcac caacggcagc ggctgataat agtggctata aaaggccagg
    15661 gcgttttcgc catttttcac tttgaacagc ggatcattcc catccacttc ggcaatcttc
    15721 acgcggcaga caccatcttc atcaatattg ccaacatagc gcaaaacttt tccttcatca
    15781 cgggccttcg ccacgcgcgc ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa
    15841 gcggcaacat caccctcggc gttaaactct gcgggcagca caggttcaat ttcaatatcc
    15901 gccagctcca gttcacgtcc cgtttcacga gcgagaatca atagtttacg cgccacatcc
    15961 ataccagaaa gatcatctcg cgggtccggt tcggtataac ccatttcccg cgccagcgtg
    16021 gtcgcctcgg agaaactcat gccttcgtct aacttgccga agatataaga aagcgaacct
    16081 gaaagaatgc cggagaactt caccaattca tcacctgcat tgagcagatt ttgcaggttc
    16141 tcaataaccg gtaatccagc cccaacgttg gtgtcataga ggaatttacg ccgcgatttt
    16201 tccgccgcat aacgcaactg atgatagtaa tccatcgacg aggtgttggc ctttttgttc
    16261 ggcgtgacaa cgtggaaacc ttcgcgcagg aagtcggcat attgatccgc cactgcctgg
    16321 ctggaggtgc agtcaacaat gaccgggttc agcagatgat attctttcac gaggcgaatt
    16381 aagcgcccga gattaaacgg ctctttggct tgtgcaagtt cttcctgcca gttttccaga
    16441 ttaaggccat gcacactagt gagcagagcc ttcgagttgg caacgccgca gacacgtaag
    16501 tcgatatgtt tattcttcag ccagctttgc tgacgcttca gttgctccag cagcgcaccg
    16561 ccaacgccac cgacgccaat cacaaacact tcgataacct gatcggtatt gaacagcatc
    16621 tgatgagtaa cgcgcacgcc agtggtcgca tcatcgttat ttaccacgac agagattgag
    16681 cgttcagaag atccctgagc aatggcgaca atgttgatat tggcgcgggc cagcgcggca
    16741 aagaatttcg ccgagatccc acgcaaggtg cgcataccat cacctaccac cgagataatg
    16801 gccagccgtt ccgtcaccgc cagcggctcc agtaagcctt ctttcagttc caggtagaac
    16861 tcttcctgca ttgcccgttc agctcgcaca cagtcgcttt gtggaacgca gaaactgata
    16921 ctgtattcgg aagatgattg cgtaatcagc accacggaaa tacgggcgcg tgacattgca
    16981 gcaaagacgc gcgccgccat gccaaccatc cctttcatcc ccgggccgga aacgctgaac
    17041 attgccatgt tattcagatt ggaaatgccc ttgaccggta attcgtcttc atcacggctg
    17101 gcaccaatga gcgtacctgg tgcttgagga tttccggtat ttttaatcag gcaagggatc
    17161 tggaactggg cgatgggggt aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa
    17221 agctccatcg cttcctggta ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc
    17281 gggtcgcagg tatagacccc gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa
    17341 caggcagcca gcaccgccgc ggagtagtcg gaaccgttac gtccaagcac caccagttcg
    17401 cctttttcat taccggcggt gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg
    17461 cttgccgcaa tacggcgggt ggactcagca atatcgacgg tagattcgag gtaatgcccc
    17521 actgccagca gtttttcgac cggatcgata acggtaacgt tgtgaccacg cgcttctaac
    17581 acgccggcca taatggcgat cgacattttc tcgccacggc aaatcagcgc agcgttgatg
    17641 ctatccgggc actgccccaa caaactaatg ccatgcagga catgttttat ttgggcaaat
    17701 tcctgatcga cgaaagtttt caattgcgcc agcgggaacc ccggctgggc ggcggcgagt
    17761 cccgtcaaaa gttcggcaaa aatacgttcg gcatcgctga tattgggtaa agcatcctgg
    17821 ccgctaatgg ttttttcaat catcgccacc aggtggttgg tgattttggc gggggcagag
    17881 aggacggtgg ccacctgccc ctgcctggca ttgctttcca gaatatcggc aacacgcaga
    17941 aaacgttctg catttgccac tgatgtaccg ccgaacttca acactcgcat ggttgttacc
    18001 tcgttacctt tggtcgaaaa aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt
    18061 cctgtacgcg tcagcccgca ccgttacctg tggtaatggt gatggtggtg gtaatggtgg
    18121 tgctaatgcg tttcatggat gttgtgtact ctgtaatttt tatctgtttg tgcgctatgc
    18181 ctatattggt taaagtattt agtgacctaa gtcaataaaa ttttaattta ctcacggcag
    18241 gtaaccagtt cagaagctgc tatcagacac tcttttttta atccacacag agacatattg
    18301 cccgttgcag tcagaatgaa aagctgaaaa atacttacta aggcgttttt tatttggtga
    18361 tatttttttc aatatcatgc agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa
    18421 gccccaggcg ttgctgtaac cagtcgacca gttttatgtc atctgccact gccagagtcg
    18481 tcagcaatgc catgactcgt tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt
    18541 cacttttcgc cggttgttgt attaatgttg ctaattgata gcaatagacc atcaccgcct
    18601 gccccagatt gagcgaagga taatccgcca ccatcggcac accagtaagc acgtcagcca
    18661 acgctaactc ttcgttagtc agcccggaat cttcgcgacc aaacaccagc gcggcatggc
    18721 tcatccatga agatttttcc tctaacagcg gcaccagttc tactggcgtg gcgtagtaat
    18781 gatatttcgc ccgactacgc gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt
    18841 cagccagtgt cgggaaaact ttaatattat caataatatc accagatcca tgtgcgaccc
    18901 agcgggtggc tggctccagg tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca
    18961 tcgttttcat tgcccgcgcc gctgccccaa tattttctgc tctggcgggt gcgaccagga
    19021 taatcgttat acgcatattg ccactcttct tgaccaaata accgcgaaca gggtgatcac
    19081 tgtcaactta ttacgcggtg cgaatttaca aattcttaac gtaagccgca gaaaaagccc
    19141 tttacttagc ttaaaaaagg ctaaactatt ttctgactgc actaacggtt taattgttaa
    19201 aaaatgctac atatccttct gtttacttag gataatttta taaaaaataa atctcgacaa
    19261 ttggattcac cacgtttatt agttgtatga tgcaactagt tggattatta aaataatgtg
    19321 acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct acaattgaac
    19381 ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta gcctgttatg
    19441 ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc tgagggaaag
    19501 tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca agaacgcaat
    19561 tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc aggcaggtca
    19621 gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa catgcagacc
    19681 ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa aagtattttc
    19741 gaagcggaag gctatgatgt tttcgaagcg acagatggcg cggaaatgca tcagatcctc
    19801 tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt
    19861 ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct gactggccgt
    19921 gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta catcaccaaa
    19981 ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg taccatgaat
    20041 ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa tggttgggaa
    20101 ctggacatca acagccgttc gttgatcggc cctgatggcg agcagtacaa gctgccgcgc
    20161 agcgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca gtctcgtgct
    20221 gaactgctga agaaaatgac cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg
    20281 acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga aatcatcgcc
    20341 accattcacg gtgaaggtta tcgcttctgc ggtgatctgg aagattaatc ggctttacca
    20401 ccgtcaaaaa aacggcgctt tttagcgccg tttttatttt tcaaccttat ttccagatac
    20461 gtaattcatc gtccgttgta acttctttac tggctttcat tttcggcagt gaaaacgcat
    20521 accagtctat attacgagtc acaaacatca tgccggccag cgccaccacc agcacactgg
    20581 ttcccaacaa cagcgcgcta tcggcagagt tgagcagtcc ccacatcact ccatccagca
    20641 acaacagcgc gagggtaaac aacatgctat tgcgccagcc tttcaatacc gcctgcaaat
    20701 aaataccgtt cattaacgcc ccaatcagac tggcgattat ccatgccacg gtaaaaccga
    20761 tatgttcaga aagcgccagc aagagcaaat aaaacatcac caatgaaagc cccaccagca
    20821 aatattgcat tgggtgtaaa cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa
    20881 aagtcagtgc aatcagcaga atggcgtact tagtcgcccg gtccgttaat tggtattgat
    20941 cggctggcgt cgtcactgcg acgctaaacg cggggaagtt ttcccagccg gtatcattgc
    21001 ctgaagcaaa acgctcaccg agattattag caaaccagct gctttgccat tgcgcctgaa
    21061 aacctgactc gctagcttcc cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg
    21121 gccagttgct ggttaaggtc atttcgctat tacgcccggc aggcaccaca gaaagatcgc
    21181 cggtaccgct taaattcagt gccatattca gctgcaggtt ctgcttccgc cagtcccctt
    21241 cgggtaaagg gatatgcacg ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg
    21301 tcagtgccgt tccgttaact tcaggcgctt tcaccacacc aataccacgc gcatccccga
    21361 cgctaatcac aataaagggc ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac
    21421 gcgaaacatc gaaatcggct tttagcgtta aatcactgtg ccagacctga ccggtataaa
    21481 tccctatctt gcgttcttcc acgttctgat tgccatcaac catcaatgac tcaggtaacc
    21541 aaaaatggat aaaacttcgt ttccgctcca cggttttatc ctcttcctgc accgtataaa
    21601 gctcggtcac aggaatagcg ataagcggcc caacgagttt ttgcggcccg ctggtacttt
    21661 ggcgaatcgc atcttccaca tcgctacggt aatcggcacg ttcgacaatc acttgccgaa
    21721 tcagcattat cggaatcaac aacagcaata ctgcaccaaa caaggtggtc attttccaga
    21781 acagggggga tttcaacata gtcttctcct ttgcaatggc agcaggatac gaaggctatg
    21841 tgggaagaat ttgaagctat gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac
    21901 cttcctgcac gttgcgcagc gtgacttcgc cgttaaacaa acgggcgacc tcactgacga
    21961 acgccaaccc cagaccgctg cttttttgcc catttgcacg cggcaaagag taaaagcgtt
    22021 caaaaatacg tgacagcgcg tagtcaggaa taccactacc ggtatccagc accttaagcg
    22081 tgacgtgttc ctgatccact tcggcgctta gcgttatgca accgctctcg ggagtaaaat
    22141 cgatggcgtt atccagtaaa ttccccagcg cctgatccag taacgccggt tcagcagcaa
    22201 cattaacctc ggtgggcata acatgcaaag tgatgttttt ttctgccaac tgcacggtgc
    22261 gcgcttcgct gacgcggcga aataatgccg ccacatcaac agcagtcaga acgacttcct
    22321 gacgattctc cagtcttgcc tggcgtagta acgtttccac cagcgcctgc attcgcgcat
    22381 tttgtgtcag aatgttgtca gtaaaacgag ccactacttc aggcggcgga ccttcgcgta
    22441 aaatttccgc cgcgccacga atcgccgcca gtgggctttt tagctcatgg gttaacgcat
    22501 aaacatactg ctcaatatag tttttccctt ccagcttcac gcgcatactt tccagcgcct
    22561 gcgcgagttt acgcagctcg ctactaccga gatcgggaag aggaacgggc ttattgtcag
    22621 tgacggaatc agcatagcga gtgagccggg caatggagcg gttgatccac caaaccatgc
    22681 ctgcgccaat caccagtgca atccccaaca aaatggcgct ggcccataaa attcgccgct
    22741 cgctgcgttt aatgaccgga gccatcgccg cgttcggttt gcctacgctc aaaacgccaa
    22801 taagccgcga gccgcccata atcggcgcgg caacatacat caccgaactt tcgggatcgg
    22861 caggattttg caacgtgctg cgcgcaccat actgaccacg caacgttagc cagacgtcat
    22921 tccagcgcga ataatcctgt ccaacggctt tatttgccga atcgaacaat actttgccat
    22981 gcgcatcggt catatagaca tgatattcat tgcgcacttt gttaatgcca ccaatattgg
    23041 cgcgaaacgg gcgatgttgt agctgattaa acgcctgcgc cagttgccca tgcgttgggt
    23101 ccccagagag caaatcggga cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg
    23161 tcccctccgt tgctcttcgc acgcccggtt taacttcttt gacaaaaata gccagtacga
    23221 accaggccgc caccgccacc agtaaaaaat agcccagcag caaccgcatg ccgatacgca
    23281 ttacaagccc ctcaggctat atcccatgcc gcgatgagta ttaatcggtg aaagatcggg
    23341 gttgatggcg cgcagcttgg cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta
    23401 ggtgtcctgc gcatcttccc acacgctatc cattagttgc tggcgggacc agacgcggcc
    23461 tggcgacttg agtaacgtct tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt
    23521 gtcaaaccag ctgatctgcg ccgcgggttc attcaattca aaatgaccaa tacggatgac
    23581 gggagacggc gtcgagaact tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac
    23641 ttcgcggggt gaaaacggtt tagccacata gtcgtcagcg ccaatttcca gcccaagcag
    23701 gcgatcgacc tcttcacttc gggccgtcag gaacagtaca ggtaacgccg gatagagcgc
    23761 cagtaactgg cggcacaatt caaagccact gatatcaggc aaaccaacgt cgagaatcat
    23821 gacgtcggga acctgctggc gagctttatc cagcaccggc aaacctcgct caaagacctc
    23881 gacggcaaaa ccttcctgct gcaacatgta gaccagcgtg tcggctatcc cttgctcatc
    23941 ttccactaac cagaccgttt cccgttgcat aaatcgcctc tgttattgcc gccacggcat
    24001 gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct acaactttgt cggagtaagc
    24061 cagataagcg agcgcgttgc gtttggcgtc ataaaagcgc acgacctgta acgacttaaa
    24121 gaccagggac gtgcgttttt tgaatactac ctcgccctga gctttgccgt ttttaatacg
    24181 atccgacagt tcaatcggcc cgacttgctg acaagaaatg gccgcatcgg aggtatcttc
    24241 tgccaggccc aatccccctt taataccacc ggttttcgcc cggctcacat aacaggtgac
    24301 atttttcaca tcgggatcgt caaaggcttc cacaacaatt ttgtgatccg ggccgatcat
    24361 tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga gccaatggcc ccagcattat
    24421 caggcttaaa gacaggatca aatgcttgta tttcatattg ttaccatttt tatctattac
    24481 acgagcaatt agttcgtcac ggtaaataac atattgatta agaacaaaat ctcaattctt
    24541 ttatttccgt caagccctaa aacatactct actaagaaaa aaacactgaa tgctaaaaca
    24601 gcaaaaaatg ctattatcca attacctgat gtcaggtgct cgttgttgaa aggatgagga
    24661 tattttatgg atcaggccgg cattattcgc gaccttttaa tctggctgga aggtcatctg
    24721 gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag gttattccaa gtggcactta
    24781 cagagaatgt ttaaagatgt cactggccat gctattggcg cgtatattcg tgctcgtcgt
    24841 ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc cgattctgga catcgcgctg
    24901 caataccgct tcgactctca acagacattt acccgcgcat tcaagaagca gtttgcccag
    24961 actcctgcac tttaccgccg ttctcctgaa tggagcgcct ttggtattcg cccgccgcta
    25021 cgtctgggtg aattcacaat gccagagcac aaatttgtca ccctggaaga tacgccgctg
    25081 attggtgtta cccagagcta ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa
    25141 atgcgttatc agttctggca cgattttctc ggcaacgcgc cgaccattcc gccggtgctc
    25201 tacggcctga atgaaacgcg tccgagtcag gataaagacg acgaacaaga ggtattctat
    25261 accaccgcgt tagcccagga tcaggcagat ggctatgtac tgacggggca tccggtgatg
    25321 ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag
    25381 tttatcctga cggtatacgg aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt
    25441 caggatattg agcgatacta cccggcagaa gacgccaaag cgggagatcg cccaattaat
    25501 ctgcgctgtg aactgctgat tcctatccgc cgttaacgct gcagctcatc taatgcaggg
    25561 gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc agccagacgc cagccacagg
    25621 ctttcctgat aatccacgcg cgaaatagaa cagttacgca gacgtaagcg gcgctctgcc
    25681 catgctggta atccgagaat cgtactcacc agacatccca gtgcaatacc atggctcacc
    25741 agcagcggtc ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc attgacgcga
    25801 tcgctgagct cctgcattga ctcgccttca ggaatacgcc cgtcaacggt gccattgacc
    25861 agctgccgac gccagttctc ttcttcttcg gtcagagaat cgatatgtct tttttccagc
    25921 acacccatgt ttaattcacg caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg
    25981 gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg
    26041 ccaagctctt tggcacgggt tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc
    26101 gggctgtcag actggccctg aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg
    26161 actaggtata cctgtaacat gctttttttc cgtatactgc tgtaacgtta attttctgag
    26221 taatgctgat tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca
    26281 ttctgcaggc atttcacgag atcttcggcg aaggatcctg ccatattgca tccgttgccg
    26341 tcgagagcgg tgtaccggaa caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa
    26401 atcgggtagc aaatgcccgc cgtttacttc cagaggctga tttttgggtg gcgattgaag
    26461 ctggcattga tggcgacagc actttcagtt gggtggtgat tgaaaacgcc agccagcgcg
    26521 gcgaggcgcg ttcggcaact ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag
    26581 gcgaggcgct tggtcccgtg agtcgcgtta taccggtatt gatgagattg gtcgtaagga
    26641 aggggcgatc ggcgtgttta ccgccgggaa actcactcgc gccagcgtct atcaccaggc
    26701 ggtgatcctg gcgctgagtc cgtttcacaa tgccgtgtat caggcattgt aggacggata
    26761 aggcgttcac gccgcatccg acattcggtg cacgatgcct gatgcgacgc tgccgcgtct
    26821 tatcaggcct acaaaatcaa tcgcttttca gcaacacctc ttccagccac tgacgcagct
    26881 ccacgggcgc ggctttcagg ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga
    26941 gttcattttt taactcacgc tgactcattt cgccacgcaa cagctcttcg acaatacgca
    27001 cgcgagtccc caacgcttcg cgctcatctg gcgtcagcat caggtttaac aacggtaaat
    27061 ggagatcgtt ttggtaggca ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac
    27121 gctgttctgc catcgctgct gaatagggtg attgttgggc cataatatgt cgccattgtt
    27181 agcgggggaa gcaaaatgac tcccccggtt gtactcgcta aagagtacga tagcatatca
    27241 taaacgtgcg gatcagtaac gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc
    27301 ccccatgaaa tagcggtagt aagcatcata agccagcacg ttcttcacat aaccgcgcgt
    27361 ctcggagaac ggaatactct cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc
    27421 aagccaggtt cgcacccgtc caggtccggc gttataagct gctgaggaga aaatacggtt
    27481 attgccaaac tgctgataaa catattgcag gtaactggtg ccaatgttga tgttcgtttc
    27541 cggatccagc aattgcccag ggctgctata accaggaata gagaacatct tcaccgtatg
    27601 ggtcgctgta ccaggcataa tctgcatcag gccgctggcc cctaccggtg atttcacttt
    27661 cggattccag gcgctctcct gacgggcaat cgccatcgca tagctttgcg ggatctcttt
    27721 cccactggtg taacgtttga aaagatcgtt gtaagccagc gggaatcgct cttccagatg
    27781 atcccaaagc ttcccggcga tcgttgcctg aacgctaaga tcccaccatt ggttattgaa
    27841 agcataccga gccagttgag cctgctctgt ttttgacttg ctcttcacca gattggccca
    27901 ctcgctacgc gcggtattat cgagattcca gtacatcaac tcgcgcacgc gcgccatctc
    27961 cggcccctga gtcagggtgc tgtcaacatt ctgcggcgct ttatcaatct tcagctcata
    28021 ctcttcaccg atgcgttgtg ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg
    28081 atgcaaaatc tctttcgctt cagcttcacg tccgcgttcc agcaataaat ccgcctgcca
    28141 gtaacgccat tcatctttct cttttgcttc catcggcaga cgcgccagcc aggtattcag
    28201 gccgcgacga tcgccggtgc caagcgccat tcgcacacgg cgttcaatca acgacgtgga
    28261 ttgcgagcgc ataatggcgt catcgcgcca tttcgcctgc tcatcggtga catcattgcc
    28321 catcaaacgc caggcgacga tatcgcgcag ctcctgaatc tgatcttcat taagctgctg
    28381 cgcctgggca agcgacggga tcatcagccg tgcattttcg gcatcctgcc gcgccacact
    28441 ggcaaacgcc accgccgcca tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa
    28501 ggtcagtacc gtattagggt tgttcgccag tgaaatgatt gccgaggcga tagtctggta
    28561 atcggcaggc atctgccctg ccagcactgt taccaggcct gtgttacccg ctttcatcgc
    28621 cagacggata cgctctaaat acgccagcgg atcttgttta cctgacgcac gccagacgct
    28681 aaataacttg tcacaggcgt taggctggct cttgccggtt agccacagct ctttcgcccc
    28741 ttgccaggct tcttcactct gcccggtgtt ccatttcgca tagtagtaat tacattgcgc
    28801 ttcggtagtt ccgggctttt ccgggctaaa ggctaacaag ccacgccagt cttcacgccg
    28861 cgccagttca ttgacgaaac gagattgcag cgtgcgagcg ggaggaagcg tggggttagc
    28921 gcgaacaaag ttagtgaccg tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg
    28981 gtattccagg tagggataaa gcggataatc cttcagtcca ggcatcattt gttcgaccac
    29041 atccatctgt cgattatccc aggcctgctt gatttgcgcg taacggctac gctgctcatc
    29101 cagtgagtcg gctcgcgcca cgctgctgac cgtcagcaga cagacaccgg cagccaacag
    29161 ccgccaggta acttgtttgg ctttttccac aagcgcatcc tctaagtgta aatacatcaa
    29221 tgcagcgtca tgccgcgtaa tagatttatg ctaaccagtc attgccgttt acgccacgtt
    29281 acggacactt ttttactttt actgcgaagg tgatcgacca actgggatta ggcggggaaa
    29341 caggctacac ttcgcctttg aaaactcatc atcacgataa acatagaggc gaagtccaac
    29401 gtggctcaat tcgtttatac catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat
    29461 attttgaaaa acatctctct gagtttcttc cctggggcaa aaattggtgt cctgggtctg
    29521 aacggcgcgg gtaagtccac cctgctgcgc attatggcgg gcattgataa agacatcgaa
    29581 ggtgaagcgc gtccgcagcc agacatcaag attggttatc tgccgcagga accgcagctg
    29641 aacccggaac acaccgtgcg tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc
    29701 ctgaaacgcc tggatgaagt gtatgcgctg tacgccgatc cggatgccga ttttgacaag
    29761 ctggccgctg aacaaggccg tctggaagag atcattcagg ctcacgacgg tcataacctg
    29821 aacgtacagc tggagcgtgc ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc
    29881 gctaacctct ccggtggtga gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa
    29941 ccagacatgc tgctgctcga cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg
    30001 ctggaacgct tcctgcacga cttcgaaggc accgtggtgg cgattactca cgaccgttac
    30061 ttcctcgata acgttgcagg ctggatcctc gaactggacc gcggtgaagg aattccgtgg
    30121 gaaggtaact actcctcctg gctggagcag aaagatcagc gtctggcgca ggaagcttca
    30181 caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg agtgggtacg tcagggaact
    30241 aaaggccgcc agtcgaaagg taaagcacgt ctggcacgct ttgaagagct gaacagcacc
    30301 gaataccaga aacgtaacga aaccaacgaa ctgtttattc cacctggacc gcgtctgggc
    30361 gataaagtgc tggaagtcag caacctgcgt aaatcctacg gcgatcgcct gctgattgat
    30421 gacctgagct tctcgatccc gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg
    30481 ggtaaatcga ccctgttccg tatgatctcc ggtcaggaac agccggacag cggcaccatc
    30541 actttgggtg aaacggtgaa actggcatcg gttgatcagt tccgtgactc aatggataac
    30601 agcaaaaccg tttgggaaga agtttccggc gggctggata ttatgaagat cggcaacacc
    30661 gagatgccaa gccgcgccta cgttggccgc tttaacttta aaggggttga tcagggtaaa
    30721 cgtgttggcg aactttccgg tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag
    30781 gttggcggca acatgctgct gctcgacgaa ccaaccaacg acctggatat cgaaaccctg
    30841 cgcgcgctgg aaaacgccct gctggagttc ccgggctgtg cgatggttat ctcgcacgac
    30901 cgttggttcc tcgaccgtat cgccacgcac atcctggact accaggatga aggtaaagtt
    30961 gagttcttcg aaggtaactt tactgagtac gaagagtaca aaaaacgcac gctgggcgca
    31021 gacgcgctgg agccgaagcg tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc
    31081 cggatgcggc gcgaacgcct tatccggcct acaaaaccag caatttcaat acgttgtaaa
    31141 actgtaggcc tgataagacg cggtaagcgt cgcatcaggc attgagcacc gaatgccgga
    31201 tgcggcgtga acaccttttc cggtctacaa cagccttagc atcctcaatt tcgtggcggg
    31261 tcacccgtgc aggttttttc aaaccttgtt taatctcgac agcctcttcc agagaggttt
    31321 tcaactcatc aaaaaaactc attttatacc tcgcctttga gcagtttcgt cacggttatc
    31381 cttgctcccc catcatctcc cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat
    31441 cgtcctcttc aacccgcacg aattcgatat tgttctcttc cagcatctcc accagcaagt
    31501 tctggaactc tttgcgatcc accgaactgc cgaggctgcg taaaccatcc gccacccacg
    31561 gcgtgttgtt ctccagcagg atcaccagat cgaaacggta ttcatcaatc agcgcctgca
    31621 cgaacggatg ctcacgccct tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg
    31681 tgtcaataaa cgccacttta ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtgac
    31741 ccaacgcgat tttatcgtag tcggaatact gcaatgcgat ttcatcaccg ccgaggtgtg
    31801 aaaagacata atcgcggcca tattcccacg cactggtggt gttgaagata ttggcaagtt
    31861 tgtttaccag ggtggattta ccgctcgact cgccgccaag gatcgccacg gtacgcacaa
    31921 aaaacggctt cacttcggta ggaatatatt cccagtagcg gaacgggttt tcgcggatct
    31981 gcgcaccgct gatactcata aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca
    32041 gatgttccat atactgtggc gcatcggctt cttccgaggt gtagatcagg tccggctgga
    32101 tccccttttc agccataaac tttttgatgc cgttgctcca cacatcccag ccatgcggat
    32161 acggctccat gccctcttcg ttgaaagcat gaatacggat atttttctga tatttaaagg
    32221 tttgcaatag ccagcgcaga cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt
    32281 caaacaacgc gcggtcgcgg gtatcgtcaa aacccataat gatatgcagc tcgtcaacct
    32341 ggctacaggc gcgctggata aggtagatat gtccggtatg cagcgggtag aacttaccga
    32401 atacgacacc aatggttttc ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg
    32461 cctccagctt ttgcgcgctg gggcttttaa ttttggcatt cagtaactgg cttaaatacc
    32521 ctttggtcat accgctggca tcagctacct gctgtagcgt gcagccctgt tgcttgatgg
    32581 cagttttcag gtaatcaaat gacgacatat ctccctccgt atctctcatt ataagtcgtc
    32641 gaacacgcta agcgcgtcgg agagtttttt aacgccaaaa atctgcatcc cttccggcgc
    32701 ttttttcggt acgttagccg ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc
    32761 agaaatccgc tcctggccac tgggcaccgg gcggatctcc cctgccagcc cgacttcacc
    32821 aaacaccacc agatcctgtg gcagcggtct gtcacgcagg ctggaaacca tcgccagcag
    32881 taacgctaag tcggcgctgg tttcggttac cttcacgccg ccgaccacgt tcacaaacac
    32941 atcctgatcg gccatttgca gaccaccgtg acggtgcaag acagccagca ggattgccag
    33001 acggttttgt tccagcccca ctgccacgcg gcgcggattc gccattatcg agtgatcgac
    33061 cagcgcctga atctccacca gcaacggacg cgttccttcc cacaccacca tcacggagct
    33121 gccggaggtc acttcatctc cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg
    33181 cagcccctgt tcggtcatcg cgaagacgcc cagctcattc accgcgccga agcggttttt
    33241 atggctgcgt aaggtgcgaa aacgggagtc ggcatcgcca tccagaagca ccgaacagtc
    33301 gatacagtgt tccagcactt tcgggccagc cagcgaacca tctttggtta cgtgtccaac
    33361 catgacaatc gccacaccgc gcgttttggc gaaacgcgtc agataagccg ccgtttcacg
    33421 cacctgcgcc acgctaccag gcgatgattg tacatccgcc atatgcatca cctggatcga
    33481 gtcaattacc atcagcttcg gttgctcttc ttcggcaatc aggcagatct gctcgatgct
    33541 ggtttccgac aacatattga gattgtcagt cggcaggcca aggcgatgag cgcgcattgc
    33601 cacctgttgc agcgactctt cgccggtgac atacagcgtt ttcatctgct gggccagttt
    33661 gcacagcgtt tgcagcaaca gcgtggattt ccccgcacca gggttaccgc caatcagaat
    33721 ggcacttcct ggcaccacgc cgcctcctag tacgcggtcg aactctttaa atccggtgga
    33781 aaaacgcggc agctcttcaa ggctgatatc ggagagtttc tggacttttg ccaccccggc
    33841 gctaccggca tagccgctga gacgctcgtt acgcgccacc gttggcgacg cagcaagacg
    33901 cacctcggtg atggtgttcc aggcatgaca ggcactgcac tgcccctgcc agcgcggata
    33961 atcggccccg cattcattac aaacaaaggc gcgttttgga gcttttgcca cggtgtacct
    34021 cgttaatgct gtgccgcccg caggatgacg ggcaggcaat tacttctgat tcaggctgcc
    34081 agagaggatg cagaataccc ccatcaggtc agcgtgacgg atggtgactt ccgccttttc
    34141 attcactttt ggcttggcat ggtaggcaat ccccagccct gctgctttga tcatcggcag
    34201 gtcattagct ccatcgccaa tcgccacggt ctgcgccagc gggatttcat actcctgcgc
    34261 gaggcgagtc agagttttcg ctttgtactg cgcgtctacg atgtcgccga tcacattgcc
    34321 ggtaaattta ccgtccatga tctccagttc attggctacc acggctgtca ggcgcagctt
    34381 gtcgcgcagg tattcagcaa agaaagtaaa gccgccggag gcaatcgcca ctttccagcc
    34441 cagcgtttcc agcttgagca ccagctgcgt taagcctggc atcagcggca gattttcacg
    34501 cacctgttgc agaatattgg cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct
    34561 ggcggtaaaa tcgagttcgc cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc
    34621 cgttccggcc agcttggcaa tttcatcaat acattcaatc tggatggcgg tggaatccat
    34681 gtccatcacc agcaaacccg gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc
    34741 cagctgcgct tcgtgggcca ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat
    34801 cacctgataa tcttccacgc accaggcggc aacaatcacc atcgccgcac ccagtttgct
    34861 ctggtattgg gtcagacgct gtttatccag cccacgacca tacagcagcc agccgctacg
    34921 acctgcgtgg taatccagtg gcatcacttc atcaccactt aatgaaagag gcagacccgg
    34981 ccataaagag acatcttcag gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt
    35041 aaaatcgttc gaagcaggga aaataacgca tgaggctacc ttgtatccat tgcttctggc
    35101 aacattaagt ctcaaatttt caaagggtgg aagatggctc gcacaaaact gaaattccgg
    35161 ctacatcggg cagtgattgt cctgttctgt cttgccttgt tagtggcact gatgcagggg
    35221 gcgtcatggt ttagtcaaaa ccaccagcga cagcgtaatc cacagctgga agaactggcc
    35281 cgcaccctgg cgcgtcaggt gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg
    35341 gatgaaaaac gcattcaggc gatcctcgat cagttaacag atgaaagccg tatcctcgac
    35401 gcgggtgtgt atgacgaaca aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg
    35461 cgcgatcggc tggcgctcga cggtaaaaaa gcaggcggct attttaacca gcagattgtc
    35521 gagccaattg cgggtaaaaa cggaccgctc ggctatctgc gcctgacact cgacacccat
    35581 acgctcgcca ccgaagccca acaggtggat aacaccacta acattttacg cctgatgttg
    35641 ctgctctcac tggcaatcgg cgtagtgctg acccgcacgc tgctacaggg taaacgcacc
    35701 cgctggcagc aatcgccctt cctgttaacc gccagcaaac cggtgccgga agaggaagaa
    35761 agcgagaaaa aagagtgacc cattactaca agaaaggaaa tcgttatgtc cacattacgc
    35821 ctgctcatct ctgactctta cgacccgtgg tttaacctgg cggtggaaga gtgtattttt
    35881 cgccaaatgc ccgccacgca gcgcgttctg tttctctggc gcaatgccga cacggtagta
    35941 attggtcgcg cgcagaaccc gtggaaagag tgtaataccc ggcggatgga agaagataac
    36001 gtccgcctgg cgcggcgcag tggcggtggc ggcgcggtgt tccacgatct cggcaatacc
    36061 tgctttacct ttatggctgg caagccggag tacgataaaa ctatctccac gtcgattgtg
    36121 ctcaatgcgc tgaacgcgct cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg
    36181 gtgaaaaccg tcgaaggcga ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat
    36241 cgcggcttcc accacggcac cttgctactc aatgccgacc tcagccgcct ggcaaactat
    36301 ctcaatccgg ataaaaagaa actggcggcg aaaggcatta cttcagtgcg ttcccgcgtg
    36361 accaacctca ccgagctgct gccggggctc accatgagca ggtttgcgag gccataaccg
    36421 aggccttttt cgcccattat ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa
    36481 cgccagactt gccaaacttc gccgaaacct ttgcccgtca gagtagctga gagtggaact
    36541 tcggtcaggc tccggcattc tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg
    36601 aactgcattt cgacgttgaa aaaggccata tcacccgcgc ccaggtattt accgacagcc
    36661 tcaaccccgc gccgctggaa gccctcgccg ggcgactgca aggctgcctg taccgcgcgg
    36721 atatgctgca acaagagtgc gaagcgctgt tggttgactt cccggaccag gaaaaagagc
    36781 tacgggagtt gtcgacgtgg atagcggggg cggtaaggta actcgctgat taaaatatcc
    36841 aaggaaggaa atatgatcga tctggaaaac caggaacggg agattatcaa tttaatgttc
    36901 agccagcgta tttcctggct agccgccgtg cgtatcaggc ataaattatc gcttgctgaa
    36961 gtaagtaaaa tgttaggtat ctcgattaat tctctaaaac agatcgaaaa gacggaacgg
    37021 cttagctcaa acatcaaaag caaaatggca gaaatttatg gttgtccacc agagttgctg
    37081 atatgccctt cctggatgac ggctgaacat aaatgaatag attcgtgaat agatacttga
    37141 atgtttttta ttttctatcc atatgaaaaa taatagtaaa tctataaatc agttgctatt
    37201 gatgaataga tactaaaaag ccggagcagt cccccactcc ggcttaacac gacaatcgcc
    37261 tttcagcgaa acacaattac tctttatcgc ccagcagaac ggattccagt gcgattttga
    37321 tcatgtcgtt gaaggtggtc tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt
    37381 ggtcagacac ggtgcagatg gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga
    37441 taccagccgc ttccatttcc acgccgagga tgccgtattt ttccatcacg tcgaacattt
    37501 cgccgtccgg agagtagaac aggtcagcgg agaacaggtt acccacgcga gcatcaacgc
    37561 ccagtgcttt agctgcatct actgcgttac gcaccatgtc aaagtcagcg atagcggcaa
    37621 agtcatggtc tttaaaacgg atgcggttaa ctttggaatc ggtgcaggcc cccataccga
    37681 taacgacgtc gcgcagtttt acgtgcggca gaactgcgcc acaggaacca acgcggataa
    37741 ttttcttcac gccgaaatcg gtgatcagtt ctttggtgta gatggagcag gacgggatac
    37801 ccataccgtg acccattacg gaaattttgc ggcctttgta agtaccagtg aagcccagca
    37861 taccgcgaac gttgttcact tcacgggcat cttcaaggaa agtttcagca atatacttcg
    37921 cacgcagcgg gtcgcctggc atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa
    37981 tgtgtggggt agccattgtt ttatcctttt tgtgacataa caaaggcgga gtcaacgctc
    38041 cgcccaaatc catcagaaca tggctttgcc atattccata tcagaagtac caaaatattt
    38101 tgccagagtc tggccgatat ccgcgaaggt ttcacggtga cccagtgagc ccggttttac
    38161 tttcgggcca tataccagta ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt
    38221 cggatcgcaa ccgtggtcag cggtgaggat caggatgtcg tcatcgcgca gcagagacat
    38281 cagctccggc agacggcggt cgaacagttc cagacccgca gcataaccgg cgacgtcgcg
    38341 gcggtggccc caggaagagt cgaagtcaac gaagttggtg aagacgatgg tgttatcacc
    38401 cgcttctttc atctctttga tggtggcgtc aaacagcgcg tccaagccag tcgctttcac
    38461 ttttttggtg ataccgcagt tggcgtagat gtctgcaatt ttaccgacag aaaccacctg
    38521 gccgtgtttt tcatcaacca gtttctgcag cacggtcggt gctggcggct caacagccaa
    38581 gtcgtgacgg ttaccggtac gctggaagtt accagctttg tcaccgataa acggacgagc
    38641 gataacacga ccgatattgt agccgccgtt ggtcagctct tcacgggcga tttcgcacag
    38701 ttcgtagagt ttatccagac cgaaagtttc ttcatggcag gcaatctgga acacggagtc
    38761 agcggaggta tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca gttgatccag
    38821 aatgaccgta ccggaagagt ggcagttacc gaggtaaccc ggcagattag cgcgttcgac
    38881 cagtttatcc agcagctctt gcgggaagct gttttcgtga tcggagaaat atccccactc
    38941 aaacagaacc gggacaccgg cgatttccca gtgaccagac ggggtatctt taccggatga
    39001 catttcgtgc gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg
    39061 aatgaaaccg gtagaacctt cgtgtgcttt cgccagcccc agacgggtca gatttggcag
    39121 attgagcggc cctttacgac cattatcagc ttcgcctttg gcacaagctt ctgcgatatg
    39181 acccagggtg tcagccccga cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc
    39241 gaatgagtcc agcaccataa taaatgcacg tttcatatgt tctccgtact cagtgcttca
    39301 aatttaaatg atcagatcag tataccgtta ttcgctgata cggcgataga cagttggtgt
    39361 gctttccggt gcgttatcgg caagtttaat tgccgctttc accgctttcg ccgcttcctg
    39421 ccagctgttt tcgtctttcg cgaggataac cgccagcgga cgctgaccgt ctacctggtc
    39481 gcccagacgc gccatatcag taaagccgac gctgtaatcg atggtgtcag atgcctgacg
    39541 acgaccgccg cccattgcaa ctactgccat ccccagcgcg cgggtatcca tttcactgac
    39601 aaaaccttcg gtatcagcat agactgcttt cgtcagcatc gctgtcggca gatacttcgc
    39661 gtagttctca acgaagtcgg tcgggccttt ttgtgccgct accatacgac caaagacttc
    39721 tgccgcttta ccgttgtcca gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt
    39781 cgccagtttg ccggagatca acatctccac gcacagcgcc atcgtgacat caaacagacg
    39841 cgggttacga tactcacccg tcaggaactg caccgcttca cgaacttcaa ccgcgttacc
    39901 tgcactggag gccagtacct gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc
    39961 gccgttagcc acgccaacaa tcgcttcggc aagggcttca gagagttcgt aggtcggcat
    40021 aaacgcgccg ctacccactt tcacgtccat caccagcgcg tccagacctt ccgccagttt
    40081 cttcgccagg atcgaggcag tgatcagcgg gatggagtcc acggttgcgg taatatcacg
    40141 ggtcgcgtag aaacgtttat cagccggagc cagtgaactg gtctgaccga taatcgccac
    40201 gccgacgtct ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg
    40261 gatggattcc agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac cggagatcat
    40321 cggaatatag ccgccgcagg ctgcgaccat cggccccaac atcagcgaag tgacatcgcc
    40381 gacgccgccg gtcgagtgtt tatcaacaat cgggccattc agatgcaggc ttttccagtc
    40441 gagaacggtt cctgaatctc gcatcgccat ggtcagcgag acacgctcag gcattgtcat
    40501 atcgtggaag aaaatggtca tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc
    40561 gcgaataccg ttgataaaga aacgaatttc ttcatcgctc agcgcatgac catcacgttt
    40621 tttacgaata atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc
    40681 ggcttttgcc ggatgcgtct acgccttatc cggcctacgg gtagatacgt ctcgtaggcc
    40741 tgataagcgc agcgcatcag gcgtaaagca tcttacttag tagctgctgg cgctcttacc
    40801 gtcgccgtgg cccagcgctt tcagcaggct tgccagcagg ctggaagcac caaagcggta
    40861 gtgacgcgca tcagcccagt cagcaccgaa cagttcatct gcgatggcga gatatttctg
    40921 cgcatcttcc gcagtacgca cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc
    40981 catatcacgg atcacttcca tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac
    41041 tttaccggta gaggttttga tgaagtccgc acccgctttg atggagattt cagacgcttt
    41101 acggatcagc gcttcgtctt tcagttcgcc ggtttcgatg atcactttca gcagtacatt
    41161 cgctgccgcg caagcctctt tacaggcttt caccaggtca aaaccaacct gctcgtcacc
    41221 cgccatcagc gcgcggtacg ggaacaccac gtcaacttca tcagcaccgt aggcgattgc
    41281 cgcacgggtt tctgccagcg cgatttcgat gtcgtcgtta ccgtgtggga agttggttac
    41341 cgtagcgata cggatttccg gggtgccctg ctctttcaga gttttgcgag caatcgggat
    41401 aaaacgagga tagatacaga tagcggcggt attgccgacc ggagttttgg cctgatgaca
    41461 cagagcaatt actttctcgt cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt
    41521 cagtgcacgc aggctgcttg ctttcagatc agtcatttca ttctcctggc ttgtcgccaa
    41581 taaaattcac cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca cacttcgata
    41641 cacatcacaa ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg atctggttca
    41701 aataattcac tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac
    41761 atcacgaaac ttcttacaat ggcgcatcac cttcggcata aaaggaaaag aagatgccca
    41821 cttctcatga aaatgcactg caacaacgtt gccagcaaat tgtcaccagc ccagtgctta
    41881 gcccggagca gaagcgccat tttctggcac tggaagcaga aaacaatctg ccttacccac
    41941 agcttcctgc cgaagcccgc cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag
    42001 gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta cgctcgtttt ctggcgaacg
    42061 gttccgaatg gctggagctg gaaggcgcga aagatcttga tgacgcactc tctctgctga
    42121 ccattcttta ccaccacgta ccgtcggtca catcgatgcc ggtctacctg gggcaactgg
    42181 atgcgttgtt gcaaccgtat gttagaattc taacacaaaa cgagatcgat gttcgaataa
    42241 aacgtttctg gcgttacctc gacagaaccc tgccagatgc ctttatgcac gccaatatcg
    42301 gcccgtctga ttcgcccatt acccgcgcga tcttacgtgc agacgcgcag gtttcaccga
    42361 acctgacctt tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga
    42421 agaacatctg tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt
    42481 tcacaaaagg gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg
    42541 gcagcacgct ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg
    42601 acttctttac gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc
    42661 ggtgtgaatt cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag
    42721 ggctgattaa ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg
    42781 ttaacttgct gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa
    42841 atgaagtggg ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga
    42901 aatatggctg gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatatcg
    42961 gcaccacgcc gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc
    43021 aaactgtcgc cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg
    43081 acgaaaccat caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag
    43141 ccggaatgcg tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt
    43201 atatggtgcg tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca
    43261 cctggctggg cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga
    43321 taagccatga acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac
    43381 gggccaggca gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt
    43441 cacaatccgt ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg
    43501 catcaggcgt tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa
    43561 tgtgatacct gcctgaagat gtgtccgcaa catgccacac caatggcgca atccatgagc
    43621 gtggacgaag tgcttagcca tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta
    43681 agcggcggcg aagccacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa
    43741 aacgatccgc aactgcgcca tctcacctgt ctggtggaca gtaacggtat gttgagcgaa
    43801 accggctggg aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg
    43861 ggtagcgaat gtcatcaaca attaaccgga cgcgataatc aacagattaa gcgcagcatc
    43921 tatttgctgg cagagcgcgg caagctggcg gaactgcgtt tgctgatgat cccaggccag
    43981 gtggattatt tgcaacacat cgaagaactg gcggcgttta tcaagggact tggtgatgtt
    44041 ccggttcgcc tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg
    44101 agcgccacgc cggaagacgt tgagccgttg gctgatgcat taaaggtgcg cggggtgagc
    44161 cggttgatat ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca
    44221 gacttgtagg cctgataaga cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg
    44281 gatgcggcgt gaacgcctta tccggcctac ggttcggcac aaacctgtag gcatgataag
    44341 acgcgttaag cgtcgcatca ggcattatgc gccgactgcc ggatgctgcc tacgaagggc
    44401 aaactaccca cgaacgttaa acaccgcata cgtgttatta agcaacactt cggcaatctc
    44461 atccgcgggt tccgggcgta actcgcaaag cacggcaaac acacgggcag cctgttccgg
    44521 gcggttaggc tgcccctgaa aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa
    44581 caacgatgcc agcggtaatt ttgcgatgac atcgcgggtt ttactggcgc gtggataggt
    44641 gatagtaccg cctacgccga ttttgtagcc cagctgtaca aaccgttcag cctgttgctg
    44701 gctgccagaa aatccgtgga caacaccagc acgcggcaaa tcgtggcgtt taagatgcat
    44761 cgccagtttg tcgtgcgtgc gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc
    44821 cagtttcagt tgttcgtcga gtaaccactg ctgcctctca aattgcggat cgtcgccgaa
    44881 gagatccaga ccgatctccc ccaccgccac caccttcgcc ggacgccttt ccagcgcctg
    44941 ctgtagctgc tcaagagaca catcgctatg tttttccaac ataccaggat gcaagcccaa
    45001 tacggcatac agcggttgat aattttccgc taacgcctgc acacgggcaa aattttccgc
    45061 ctctgtcgcc ggaacaatga tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc
    45121 ctcttcatcg ccaataaaag gcgggaaatc aaaatggcag tgagtgtcga taaaacggca
    45181 aatcaagcca gatcctcatt gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact
    45241 tcggcaacca gcgtatcgtt ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc
    45301 accactggca ccagatggcg ggtaagcggc gctttttcag ttaatagctt gccaaccgtg
    45361 gcgaggaaat aacgaccgca taaacgccca agtttatagt cttcacgcag cgccggaatc
    45421 cgactgccaa gcgcgatact atgtaatggc ttcggcggat aaatttcgga tatccgcagc
    45481 ttgcccggtg gtttctcaat aaattgctga atgtcacgat agctggtttc atgatgctgc
    45541 accagattga ccagcggctg caggctactg tcacccagcc agcgttccat gcgtttgaac
    45601 cactgcggcg tgtagtacat ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg
    45661 ccctgccttg ccgcctcttt aaccggaatc gcatcactga tcccgccatc caggtagtta
    45721 atgccttcca gcgacactcc gctacgataa aagccaggta tcgcactgga ggcgcgaatc
    45781 acatccagcc agttttgttt ggttggtaaa aagtaattcg gcgcgtagtc atcctgacga
    45841 caggcgcaca tataaaacga tttgccgctg tcaaacaacc gcgcggcggt atccatttgc
    45901 aacggcatct ggcttgcggt ggcctccacc agccagtcga gatcgataag atttcctcca
    45961 cggacaaagc gcaatggatc gaaaaattcg cgttttgtgg tatagcgcat gatgactttg
    46021 cgcgcgtaac cgggctgatt gcagataaac gccgagaggt tctgcgcccc ggcagatgtg
    46081 ccgagataaa gatcgaaagg attaaactgc gcgcgcataa actcatccag cacgccagcc
    46141 gtgaaaattc cacgctgtcc gccgccttcg cacaccagag ctattcgtcc aggctggaac
    46201 ggccttagcg acaacggcgc aatattacca agcgttacag gtattcgctg ccccacttct
    46261 gccttcctgt ttttgttgtg aaagaaagac aaagtaacgc aatttattat tcgctaaaac
    46321 cgcaaaagcc agtccgctgg actggctttg gataaaattt tcttaatggt ctgaaatcta
    46381 gggtcgtttt cggcccatga acaaactcac taggaacaga ataatcccga cgacaaagac
    46441 aattttagct gcgcctgcag cggtaccggc cagaccacca aacccaagtg cggcggcgat
    46501 taacgcgata accagaaata tgatgcccca acgaaacata agcgtctcct ttaccatagt
    46561 taatgtcacc gcttgatatg agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc
    46621 acatcgcgcc tcccgacaaa gttcgggagg acgaattact tagttttcag atcattttta
    46681 acgcttttca caccatctac cgctttggcg atactttcag cacggtcact ttgtgcctga
    46741 gaatctacgg taccggagag ctgaaccacg ccatcggtgg tttcaacttt cactttacgt
    46801 gaagggacga tatcgtccgc cagcagtttg gctttgattt cactggtggt ggcggtgtca
    46861 cccgcgtagc ccttcaccga gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca
    46921 gaggtcacgc cttcaacgcc tttcgccact ttcactgcct cttcggcctg ggcctgactt
    46981 tcaacgaaac cgctcagggt cacgactttt tgatcggttt tgacagagat atcggtgctc
    47041 ttgatgttgt catgatccac cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc
    47101 atgaaattac cgactttatt catagagcta tcgacttttt gccctgcgct ttcattggta
    47161 gtctgcgcgt tgttttccgc gtaggcagag ccggtcgcga cggcagaggt caacattaca
    47221 gccagcagag ttttcgaaat cttcagtctt gtcatagtca tcgatttatt cctgtatgtt
    47281 tgctcgtaat ttgagcctag caacacgagg ttgcattgct gaatagggag agacttcacc
    47341 ctctacagaa gtcaatggtc gccatcacaa aagcgatggg tgatgaataa cgaccattac
    47401 agcctctgaa tcagttatta atatcggtag aatgacaatc gacggcttta gatactaata
    47461 tctacgcatt gaacggtatt taacgccatc agaaatgtca tcactttgtt aaatatagat
    47521 cacaattttg aaaccgctcg ggatatcagc gaaaacataa gcaaaagtga atgttttaag
    47581 aacattccgt aagcggctaa taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc
    47641 ctggaggcaa gaattaatgc tcgcgggtct ggtggaactg aacgtccgga taacgttcct
    47701 gtgccaggcg caggttgacc atgctggtag cgatgtaagc gaggttatcg ccgccatcaa
    47761 gcgccagttg gctttcgttc ttacgcttga actcttcgaa tttcttcgcg tctgcacatt
    47821 ctacccagcg ggcagtggca acgttgactg actcatacac tgcttcaacg ttgtattcgc
    47881 tcttcaggcg cgctaccacc acatcaaact gcagcacacc aaccgcacca acgatcagat
    47941 cgttgttgga gattggacgg aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc
    48001 ctttgagcag ctgtttttgc ttcagcggat ctttcaggcg gatacgacgg aacagttctg
    48061 gtgcgaagtt cggaataccg gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga
    48121 tctgaatggt gccgtggttg tgcaggccga ggatatcgcc cggatacgct tcttcaacgt
    48181 gcgaacggtc acccgccata aaggtcagcg cgtcggagat caccacatct ttcgcagtgc
    48241 gcacctggcg cagtttcatg cctttttcat atttaccgga caccacacgc ataaacgcca
    48301 cgcggtcgcg gtgtttcgga tccatgttgg cctgaatttt aaatacgaag ccggtaaatt
    48361 tatcttcgct cgcttctacg gtacgggtat cggtctgacg cggcatcggc gcaggcgccc
    48421 actccaccag gccattcagc atatgatcaa cgccgaagtt acccagcgca gtaccgaaga
    48481 ataccggagt gatttcgccc gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt
    48541 taaccagttc cagttcgtca cgcagctgct gtgccagatc ttcaccaacc gcagcatcga
    48601 gatccgggtt attcagccct ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc
    48661 tctgatagag ataggtttca tctttataaa ggtggtaaac gcctttaaac agcttgccgc
    48721 agccaattgg ccaagtgatc ggtgcgcagc caattttcag ctcgttctca acttcatcga
    48781 gcagttccat cgggtcgcgg atatcacggt caagtttgtt cataaaggtg aggatcggcg
    48841 tgtcgcgcag acgggtaact tccatcagct tacgggtacg atcttcaaca ccttttgcgg
    48901 cgtcgataac catcaggcag cagtccaccg ccgtcagggt acgataggta tcttccgaga
    48961 agtcttcgtg ccccggggtg tcgagcaggt taaccaggca atcgtgatac ggaaactgca
    49021 tcacagacgt agtaatggag atcccacgct gcttttccat ctccatccag tccgacttag
    49081 cgtgctggtt ggaaccacgg ccttttactg taccggcggt ctgaatggcc tgtccgaaca
    49141 gcagcacctt ctcggtgatg gtagttttac cggcgtccgg gtgagaaata atggcaaaag
    49201 tgcggcgctt cgccacctct ttcaaataag gagacaacgt cataattctt tcttcttgag
    49261 taagcggcat cgcgccgcgc ttgttgaaaa cgaaaaattg cggctatttt acccatcaac
    49321 gggggggagg caatcagtgt ttacacagga gctgctccag ttcgtgcaac gaagaaacgg
    49381 tccaggtggg cgcgatgcct tctggttgct cgcgattgtg cgcattcagc cagcaggtcg
    49441 caagcccggc gttgatgcca ccgagaatat cggactcggc agtgtcgcca accatcagca
    49501 cgcgtgaacg gtcaggattg cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg
    49561 gtttggcaac gccaacttct tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg
    49621 tccgttccag acgcacttgc tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag
    49681 cgttgctacg aatggcgtta agcagagaaa ccgcgcccgg caacggcgta cagatttccg
    49741 ccatcgcatt aataaaggca tcgttaagca agcctgcttc aactttcagc cgttcggccc
    49801 agctctcgaa acgcccgtgc tgaagctgta atgaagtgat cgcgccgttt tgataatcca
    49861 cccacagtgg cttgttaacg gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc
    49921 tgtaatcaag aaacatccgc tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat
    49981 cggcatcaaa gaaaatccag tcccacttca ttattccacc ttgtcttaca tactgattgg
    50041 caacgccatg atgatggcgt cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat
    50101 cgtcgcctcg ttaaagccta aactttcgta cagggcaatg gcggcagcat ttgaggcacg
    50161 gacttccagc catagtgtcg ccacgccgcg tttttccagt tcgtcgatca gatgttccag
    50221 cagcgcccta cccagcccct gacgctgata gtcgggatcg accgcaatat tgaacaatgt
    50281 agcttcatcc agcaccactt gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa
    50341 ctgaaagttg agataacgct cgccctggtt gctggcaaag gttttttcac tccacggaaa
    50401 ggcgtgggcg cgttgttcaa tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga
    50461 aatcgtgttc atatgtgcaa atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc
    50521 gtaattcggt gagcgccggt gatgccacct gagcgccttc caatgatagc ggttcgtcag
    50581 tacccaaccg ccagctgttg cagcgactgc cttgcggcag catcgcgatt ttttctggcg
    50641 tcagttgcag cacttgatcg gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag
    50701 gatcagtcag ggcgggaaga tcgtttgcca ccatcaccag acggacgtgt gccgggctcg
    50761 caatggcaat ctccccctgc aacgcgccag ggcgacgcag cgaccactgg gtaatgccca
    50821 gttgctgtaa ctgccagtct cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc
    50881 gcaaatatag caaattcgtc gataccgcgc caacatatgg ctataatcgc cgccagtatc
    50941 aattgaggag cattccatgt ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag
    51001 tgatgatttc gaacaaagcc gtattctgtt tgccggagac ttacaggatg acctgcccgc
    51061 gcgtttagat accgcggcca gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt
    51121 aagctgccag atgggggata acgcccgttt tagtctggtc gccacggcga atgatgtcgc
    51181 agattgcgat acgctgattt actactggcc gaagaacaaa ccggaagccc agttccagtt
    51241 gatgaattta ctttctctgc tgccagtggg gacagatatt tttgtcgttg gcgagaaccg
    51301 cagcggcgtg cgcagcgctg agcagatgct ggcagattat gcgccgttga ataaagtcga
    51361 cagcgctcgt cgctgtggcc tctattttgg tcgtctggaa aaacagccag tatttgatgc
    51421 ggataaattc tggggcgaat acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt
    51481 gtttagccgc gacggtctgg atgtcggtag ccagttgctg ctctcgacgt taactccgca
    51541 cacgaaaggt aaagtgctgg atgtcggctg tggcgcgggt gtactttcag ttgcctttgc
    51601 gcgccattcg ccgaaaattc gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc
    51661 cagccgcgca acacttgcag ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt
    51721 ttccgaggtg aaaggttgtt ttgatatgat catctccaac ccgccgttcc acgatgggat
    51781 gcaaaccagc ctggatgcgg cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag
    51841 cggcggcgag ctgcgaattg tagcgaacgc cttcctgcct tacccggacg tgctggatga
    51901 gacatttggc ttccatgaag ttatcgcgca aaccgggcgc ttcaaggtat atcgcgccat
    51961 tatgacccgc caggcgaaga aaggttgagt atctgatatc tgtcgccgca taccgtgtcg
    52021 gatgcggcgt gaacgcctta tccgacctac aaaacctgca cgttagccct tcgtaggcca
    52081 gataagacgc gccagcgtcg catctggcat ctgcccccgc atgcctacca aacctgcacg
    52141 ctagtttcct gatggacatt tttccagcaa ttacacatca ggcgataatt aactattgac
    52201 gaaaagctga aaaccactag aatgcgcctc cgtggtagca attcttttta agaattgatg
    52261 g
//
LOCUS       DAJTRM010000018        49221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRM010000018 DAJTRM010000000
VERSION     DAJTRM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.018"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..840
                     /locus_tag="OO322_001133"
     CDS             <1..840
                     /locus_tag="OO322_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5906092.1"
                     /translation="PMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLACAGPMGIG
                     AIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGMSDPIQM
                     LRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFNFYNVHY
                     NSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIPGGKK
                     LIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDIGE
                     AAQ"
     gene            837..1802
                     /gene="yggF"
                     /locus_tag="OO322_001134"
     CDS             837..1802
                     /gene="yggF"
                     /locus_tag="OO322_001134"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5906093.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1824..2333
                     /gene="fumE"
                     /locus_tag="OO322_001135"
     CDS             1824..2333
                     /gene="fumE"
                     /locus_tag="OO322_001135"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5906094.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2330..3043
                     /locus_tag="OO322_001136"
     CDS             2330..3043
                     /locus_tag="OO322_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5906095.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3015..3692)
                     /locus_tag="OO322_001137"
     CDS             complement(3015..3692)
                     /locus_tag="OO322_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5906096.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3686..4363)
                     /locus_tag="OO322_001138"
     CDS             complement(3686..4363)
                     /locus_tag="OO322_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5906097.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4351..4734)
                     /locus_tag="OO322_001139"
     CDS             complement(4351..4734)
                     /locus_tag="OO322_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5906098.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4750..5058)
                     /locus_tag="OO322_001140"
     CDS             complement(4750..5058)
                     /locus_tag="OO322_001140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906099.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5059..5637)
                     /locus_tag="OO322_001141"
     CDS             complement(5059..5637)
                     /locus_tag="OO322_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5906100.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5660..6091)
                     /locus_tag="OO322_001142"
                     /pseudo
     CDS             complement(5660..6091)
                     /locus_tag="OO322_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6463..7482
                     /gene="epd"
                     /locus_tag="OO322_001143"
     CDS             6463..7482
                     /gene="epd"
                     /locus_tag="OO322_001143"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5906101.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7532..8695
                     /gene="pgk"
                     /locus_tag="OO322_001144"
     CDS             7532..8695
                     /gene="pgk"
                     /locus_tag="OO322_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5906102.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8910..9989
                     /gene="fbaA"
                     /locus_tag="OO322_001145"
     CDS             8910..9989
                     /gene="fbaA"
                     /locus_tag="OO322_001145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5906103.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10180..11040
                     /gene="mscS"
                     /locus_tag="OO322_001146"
     CDS             10180..11040
                     /gene="mscS"
                     /locus_tag="OO322_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5906104.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11179..11814
                     /gene="argO"
                     /locus_tag="OO322_001147"
     CDS             11179..11814
                     /gene="argO"
                     /locus_tag="OO322_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5906105.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11907..12647
                     /locus_tag="OO322_001148"
     CDS             11907..12647
                     /locus_tag="OO322_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5906106.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12824..13774
                     /locus_tag="OO322_001149"
     CDS             12824..13774
                     /locus_tag="OO322_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5906107.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13716..15194)
                     /gene="scpC"
                     /locus_tag="OO322_001150"
                     /pseudo
     CDS             complement(13716..15194)
                     /gene="scpC"
                     /locus_tag="OO322_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15218..16003)
                     /gene="scpB"
                     /locus_tag="OO322_001151"
     CDS             complement(15218..16003)
                     /gene="scpB"
                     /locus_tag="OO322_001151"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5906108.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16014..17009)
                     /gene="meaB"
                     /locus_tag="OO322_001152"
     CDS             complement(16014..17009)
                     /gene="meaB"
                     /locus_tag="OO322_001152"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5906109.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17002..19146)
                     /gene="scpA"
                     /locus_tag="OO322_001153"
     CDS             complement(17002..19146)
                     /gene="scpA"
                     /locus_tag="OO322_001153"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5906110.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19350..20243)
                     /gene="argP"
                     /locus_tag="OO322_001154"
     CDS             complement(19350..20243)
                     /gene="argP"
                     /locus_tag="OO322_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5906111.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20626..21285
                     /gene="rpiA"
                     /locus_tag="OO322_001155"
     CDS             20626..21285
                     /gene="rpiA"
                     /locus_tag="OO322_001155"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5906112.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21541..22773
                     /gene="serA"
                     /locus_tag="OO322_001156"
     CDS             21541..22773
                     /gene="serA"
                     /locus_tag="OO322_001156"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5906113.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23002..23061
                     /gene="ibsC"
                     /locus_tag="OO322_001157"
     CDS             23002..23061
                     /gene="ibsC"
                     /locus_tag="OO322_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5906114.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23394..23453
                     /locus_tag="OO322_001158"
     CDS             23394..23453
                     /locus_tag="OO322_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5906115.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23554..24156)
                     /locus_tag="OO322_001159"
     CDS             complement(23554..24156)
                     /locus_tag="OO322_001159"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5906116.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24177..24360)
                     /gene="ssrS"
                     /locus_tag="OO322_001160"
     ncRNA           complement(24177..24360)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO322_001160"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24402..24731)
                     /gene="zapA"
                     /locus_tag="OO322_001161"
     CDS             complement(24402..24731)
                     /gene="zapA"
                     /locus_tag="OO322_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5906117.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24899..25477
                     /gene="ygfB"
                     /locus_tag="OO322_001162"
     CDS             24899..25477
                     /gene="ygfB"
                     /locus_tag="OO322_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5906118.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25503..26828
                     /gene="pepP"
                     /locus_tag="OO322_001163"
     CDS             25503..26828
                     /gene="pepP"
                     /locus_tag="OO322_001163"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5906119.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26825..28003
                     /gene="ubiH"
                     /locus_tag="OO322_001164"
                     /gene_synonym="visB"
     CDS             26825..28003
                     /gene="ubiH"
                     /locus_tag="OO322_001164"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5906120.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28026..29228
                     /gene="ubiI"
                     /locus_tag="OO322_001165"
                     /gene_synonym="visC"
     CDS             28026..29228
                     /gene="ubiI"
                     /locus_tag="OO322_001165"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5906121.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29302..29385)
                     /locus_tag="OO322_001166"
     CDS             complement(29302..29385)
                     /locus_tag="OO322_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906122.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29676..30770
                     /gene="gcvT"
                     /locus_tag="OO322_001167"
     CDS             29676..30770
                     /gene="gcvT"
                     /locus_tag="OO322_001167"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5906123.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30794..31183
                     /gene="gcvH"
                     /locus_tag="OO322_001168"
     CDS             30794..31183
                     /gene="gcvH"
                     /locus_tag="OO322_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5906124.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31302..34175
                     /gene="gcvP"
                     /locus_tag="OO322_001169"
     CDS             31302..34175
                     /gene="gcvP"
                     /locus_tag="OO322_001169"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5906125.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34442..35185
                     /locus_tag="OO322_001170"
     CDS             34442..35185
                     /locus_tag="OO322_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5906126.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35242..36675)
                     /gene="bglA"
                     /locus_tag="OO322_001171"
     CDS             complement(35242..36675)
                     /gene="bglA"
                     /locus_tag="OO322_001171"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5906127.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36720..37030
                     /locus_tag="OO322_001172"
                     /pseudo
     CDS             36720..37030
                     /locus_tag="OO322_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37194..37853
                     /locus_tag="OO322_001173"
     CDS             37194..37853
                     /locus_tag="OO322_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5906128.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38049..39029)
                     /gene="ygfZ"
                     /locus_tag="OO322_001174"
     CDS             complement(38049..39029)
                     /gene="ygfZ"
                     /locus_tag="OO322_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5906129.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39272..39538
                     /gene="sdhE"
                     /locus_tag="OO322_001175"
     CDS             39272..39538
                     /gene="sdhE"
                     /locus_tag="OO322_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5906130.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39519..39926
                     /locus_tag="OO322_001176"
     CDS             39519..39926
                     /locus_tag="OO322_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5906131.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39966..40487)
                     /gene="fldB"
                     /locus_tag="OO322_001177"
     CDS             complement(39966..40487)
                     /gene="fldB"
                     /locus_tag="OO322_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5906132.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40599..41495
                     /gene="xerD"
                     /locus_tag="OO322_001178"
     CDS             40599..41495
                     /gene="xerD"
                     /locus_tag="OO322_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5906133.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41520..42230
                     /gene="dsbC"
                     /locus_tag="OO322_001179"
     CDS             41520..42230
                     /gene="dsbC"
                     /locus_tag="OO322_001179"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5906134.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42236..43969
                     /gene="recJ"
                     /locus_tag="OO322_001180"
     CDS             42236..43969
                     /gene="recJ"
                     /locus_tag="OO322_001180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5906135.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44060..45158
                     /gene="prfB"
                     /locus_tag="OO322_001181"
     CDS             join(44060..44134,44136..45158)
                     /gene="prfB"
                     /locus_tag="OO322_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5906136.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45168..46685
                     /gene="lysS"
                     /locus_tag="OO322_001182"
     CDS             45168..46685
                     /gene="lysS"
                     /locus_tag="OO322_001182"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5906137.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46728..47276)
                     /gene="idi"
                     /locus_tag="OO322_001183"
     CDS             complement(46728..47276)
                     /gene="idi"
                     /locus_tag="OO322_001183"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5906138.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47331..47402
                     /locus_tag="OO322_001184"
     CDS             47331..47402
                     /locus_tag="OO322_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906139.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47399..47524)
                     /locus_tag="OO322_001185"
     CDS             complement(47399..47524)
                     /locus_tag="OO322_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906140.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47526..48974)
                     /gene="uacT"
                     /locus_tag="OO322_001186"
     CDS             complement(47526..48974)
                     /gene="uacT"
                     /locus_tag="OO322_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5906141.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11876 a        12335 c        13257 g        11753 t
ORIGIN      
        1 cctatgtgct gcattattgg tgcttatcat gccaattatc acaccacgca atatgtttat
       61 gagcatcgca tgggcgtcaa acctggcggc aatattgcac tgctggcgtg tgcgggtccg
      121 atgggcattg gcgctatcga ttacgccatt aacggtggca tacaaccgtc acgggtggtg
      181 gtggtcgata tcgacgacaa acgcctggcg caggtgcaga agctgctgcc ggtggatctg
      241 gcggccagta aaggcattga gctggtgtat gtgaatacca aagggatgag cgatcctatc
      301 cagatgctgc gggcgctgac aggagatgtc gggttcgatg acatttttgt ttatgcggcg
      361 gtgcctgctg tcgttgagat ggctgacgaa ttactggcgg aagatggctg tctgaacttc
      421 tttgccgggc tgacggataa aaacttcaaa gtgccgttta atttctacaa cgtccattac
      481 aacagcacgc acgtagtcgg tacatccggc ggttcaacgg acgacatgaa agaggcgatt
      541 gcccttagcg ccacaggaca gttacagccg tcgtttatgg tgacccatat tggtggcctg
      601 gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc ctggcggtaa aaaactcatt
      661 tataacggcg tgaccatgcc gctcactgcc attgccgatt ttgccgaaaa aggcaaaacc
      721 gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa cgcacggcat ctggaatgaa
      781 caggccgaga aatatctgct ggcacaattt ggcgttgata tcggggaggc cgcgcaatga
      841 tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct gcctggccgc
      901 aaaccggatg tggcgacaaa aataaaattg atggcctggc ggtcaccgcg atgcgccagg
      961 cattaaacga cgtcgctttt cgtggacgag tggttatcgg cgaaggagaa attgaccatg
     1021 cgccgatgct gtggattggc gaagaggtgg gcaaaggcga tgggccagaa gtcgatatcg
     1081 cggttgatcc cattgaaggc acgcggatgg tggcgatggg gcagagcaat gcgctggcgg
     1141 tcatggcttt cgctcctcga gatagcctgt tacatgcgcc cgatatgtat atgaaaaagc
     1201 tggttgttaa tcgactggct gccggggcga ttgatctgtc tttgcctctg gcggacaact
     1261 tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa gctgcgcatg gttacgctgg
     1321 ataaaccgcg tctgagtgcc gcgattgaag aagcaacaca gctgggcgtg aaggtttttg
     1381 ccctgccgga tggcgatgtc gccgccagcg tgctgacctg ctggcaggat aatccttacg
     1441 atgtgatgta caccatcggc ggtgcgccag aaggcgtgat ttctgcctgt gccgttaaag
     1501 cgttaggcgg ggatatgcag gccgagctga ttgacttttg tcaggctaaa ggggattaca
     1561 cggaaaatcg gcagattgca gagcaggagc gcaagcgttg taaggcaatg ggcgttgacg
     1621 ttaaccgtgt gtactcgctc gatgaactgg tgagggggaa cgatatcctc tttagcgcta
     1681 cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca gacggcgaat ggggtgcgga
     1741 cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa tataatagac tccctgcatt
     1801 gatggtgatc tatcggaaga ccaatggcga cgctgacaga agatgatgtg cttgagcaac
     1861 tggatgcaca ggacaattta ttttcattta tgaaaactgc gcattctatt ttgctccagg
     1921 ggatacgcca gtttctgccg tcgctgtttg tcgataacga tgaagagatc gtcgaatatg
     1981 cagtgaagcc gttactcgcc caaagcggcc cgcttgacga tattgatgtt gcgctgcgtt
     2041 tgatttatgc gctggggaaa atggataaat ggctgtatgc cgatatcacg cacttttccc
     2101 agtactggca ttacctgaac gaacaggatg aaacgcccgg atttgccgat gatataacct
     2161 gggattttat cagcaatgtc aacagtataa ctcgcaatgc gacgctctac gatgcgttaa
     2221 aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc gcgttttagc ggaatggtca
     2281 aaacggcgct gacgctggca gtaacgacca ccttaaagga attaacgccg tgaaaattga
     2341 attaacggtg aatgggctga acgtgcaagc gcagtaccat gatgaagaga tagaacgcgt
     2401 gcataaactg ttgctgcgta tgctggcggc cttacagacg gtaaatccgc agcggcgtac
     2461 ggtggttttt ctttgcgctc cgccggggac gggtaaatct accctaacca ccttctggga
     2521 atatctcgcg caacaagacc cggagctgcc cgctattcag gcgctcccga tggatggttt
     2581 tcaccattac aatagctggc tggacgcgca tcaattgcgc cccttcaaag gcgcaccaga
     2641 gacatttgac gttgcgaaac tggcggaaaa tctgcgccag gtggtggaag gggattgtac
     2701 gtggccgcag tacgatcgac aaaagcatga tcctgttgaa gatgcgttgc acgttaccgc
     2761 accactcgtc atcgtcgaag gaaactggtt gttgctggac gacgagaagt ggtgccagct
     2821 tgcgcaattt tgtgatttct cgatctttat caacgcgcca gctgctgcgt tgcgggagcg
     2881 tctggtgggg cgcaagctgg cgggaggggt atcgctggca gatgcagaag ccttttatga
     2941 ccgtaccgac gggccgaatg ttcgccgggg gctggaagag agtctgcctg caaatttgac
     3001 cttaatgatg accgctacgg gtgaatatcg tctggtggat taacgttcct gattacacca
     3061 tcttccagcc gcaccacacg agagaaatga cgccgggtaa atgcggcgtc atgactgatt
     3121 gctaccacgc tcgtgcctcg ctggcggcac ttctccagcc agctttcaaa cacgctcaac
     3181 caattttcgt caaaatcccg actcggctcg tccagcagta atagtggcgg agagagtgct
     3241 tccaggcagg cgacggcgac catccgtcgc tgcgcattat gcagatccaa cgggtgcgag
     3301 ttggcagtgt ctgctaactg acaacattgc agggcagcat gggtacgttg agtgatttca
     3361 tccgccgggc atttttgcag tttcaaacca aatgcgatct cgtcagcgac ggtgctgtga
     3421 aaaagttggt tttccgcttc ctgaaacagt acgccaacct ttgccgcgcg ctggcggttt
     3481 ttcaggtttt tcatcgcctg ttgttgcagc ataaccgtac cggctgtggg cgtaagaaga
     3541 cctgccatta ctcgtaacag cgtcgatttc cctgcgccat tgtcaccggt cagcgccaac
     3601 cattcacctt gtttgagttg cagagaaatg tcgcaaagac aatccgttgc cgcgccaggc
     3661 cagcgataag atatcttatt taacgttaac atagtggctg gagcgctccg ttttgcaatt
     3721 gccacgcttg ctggcagtag ttcaggaaag gtgtgcgatg acgttcaaaa agaataatca
     3781 gcgaaccacg ttcaaacgcc cagtgttgta agcgctgcaa cagcatttcg ctggcctgcg
     3841 gtgtcaggcg gctaaaggct tcatcaagaa ttaagagctt tggctgcatc gcgatggcgc
     3901 aggcgatgat cacgcgctgt gtctctccac cggagagtgt tgccgggtga cgatggcgca
     3961 acggctggca ctcggtcaga gtcagggcgg catcaatccg cgccataatc tctgcttcag
     4021 caagacacag attttctggc ccaaaggcaa cttcttcttc cacgctaaag gtacagccgg
     4081 agagctgcaa atagggcgat tgctggacga gctggatggt ggcggattgt tcattaagcg
     4141 ggagatgccc aatgggcgta ccgaggagcg tccccgtgcc tgttatctct cttggcagaa
     4201 agtccggata ccagcccgcc atcagctgtg ccagcgtact tttgccactg ccgttgtcac
     4261 caaagatggc gaccatcccc ggtttgacat agtgaaagtc ataacagaac ggaggatgcg
     4321 ttgagtgtgt cgggcaataa cggaactgtt ctaacgtaac catatccacg ctccgaattc
     4381 tgtcagcatc agcaagatca tggtgtaacg cgctacgcgt tgtagggtgc tatcggctgg
     4441 tgcccacaac gttgttcgtc ggttgtggat ccgaaatgcc cgcatatcca gcgccgcacc
     4501 acggacagcc agatcgttaa gcgcattgtg tgttagcgga ataattaacg cgggcatagc
     4561 acgtaaacgc tgataccagc cctcatccag cggcacgccg cgggcacgct gcgcttcatg
     4621 aataatcgct aattgccgtt ttaactgctc aacgaccaat aacggcccgg caaacagata
     4681 agcgacgcct ggcggcaggc gagaagcaaa cagagcgcga ataaaacatt gcacggggac
     4741 gtactgcatt cacaactgtg aggttgaaac aatcgccaga atcagcaacc agagcgtaat
     4801 agcatgcgac caccgttctg gcgagcgtgg cgtgccgctt agccattctg gtagccagcc
     4861 accgtgtacc agccacagcc cggctcccag cgagaacatc agccagacga catattttgc
     4921 ccgccgccgc gtggccttta gcgcaataag gcagaaaaag gtcgccgcgc tatagatcgg
     4981 caaaatggtc tgcgcgggca ggataagcgt ggtgcaggcc gctaatgccc acagtgttaa
     5041 tgaggtaaat ggatgcattt agcgcacggc agccattgcc ggaaaatggc gcgtggtgcg
     5101 aatcggcagc tggcgcagta aaagccagac aatcaccgct gtaagaattt tatcgaccag
     5161 gttcgcgcct atcaccgtaa tcgccaccga ctccaccagg ttttgcccca tcgagtgcat
     5221 ccaggcaacg aacaaatcgg ctccgctacc ggtgacaccg ccgaacagcg cagtacgcag
     5281 cggtacagcg acgaccgtta ccgcaagcgt aataatcacg ccgctgacaa cgacttttgg
     5341 cagggtgcga aaccaacccg ctcgcgccag ccaaccggag accaggccga tgaccatcgc
     5401 gacgggtgca aatgcggcgg cgatcgggtc ggtcagcaat ccccagagta aattggtcag
     5461 taagccggtc agcattccga tcaccggacc aagcagtacc gcgctaataa gtgtgccgat
     5521 ggagtcgaga aatataggta atttgaccat gctggccagt tgcccgccga tcatattgat
     5581 ggcgatagat ataacgatca acaccagtgc ctgactggaa aagtggctac gcgccataag
     5641 aaaacctctt tgaaagtagt tatttaacgg gggaaatatg caactcgtcg taaccagaaa
     5701 gcgcgcaagg ttggcggcta aacgccagtt cctcaaggct accgatcacc agttcaagcg
     5761 tggtccgcac ggtacagccg ggcatcatat gaccgccaag catcgtgccg tgtggatcgg
     5821 aaacgcagag atgaagatgc tcaccactct gttccagcgt gccgtttagt gagatcactt
     5881 cgaatgtacc gttcaggagc gtggtgcctt cttgcccggc atagcgcaag gcgacgtcag
     5941 tcaggctacc ggtacagcct gcaatctagg cggcatgaag ttgatgttgc tgtgcaaaag
     6001 cgtgtagctg ggaaaaaact tcctgcccgg ggaggaggcg cagtgcgtaa aagcgtgcgg
     6061 aggaagaata tgagtagggc aatgtcatca ttggagtact ccgaaactcg gacgtattgc
     6121 ggataatatc gacgctgcat cagtcatatt ctgatataag tcagggtttg tgacggcaca
     6181 acgaaaagat tttgctacgc ttattcgtga ttctgcaaag tggaataaat tccacagggg
     6241 cggggctgaa aacgtgacgt aagtcagata attgctttct ttgactggac aatcattcct
     6301 tttattccac gtttcgctta tcctagctga agcgtttcag tcgattaaat gttcgacaat
     6361 taaccaatca gtcgcagttt gcgacaggta aggtttcccc ggacgatttg ctggattact
     6421 ctgtctggca aatttgctca gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg
     6481 ataaatggct tcggtcgcat cgggcgtaat gtggttcgtg ctttgtatga atccggacgc
     6541 cgggcggaaa ttaccgtggt ggcaatcaac gaactggcgg atgctgcggg catggcgcat
     6601 ttgttgaaat atgacaccag ccatggccgt tttgcatggg aagtacgaca ggaacgcgat
     6661 caactttttg ttggtgatga cgccatccgc gtattgcatg aacgttcact gcaatcgctc
     6721 ccctggcgtg aacttggcgt tgatgtagtc ctcgactgca ccggcgtata tggctcccgc
     6781 gagcatggcg aagcgcatat tgccgccggg gccaaaaaag tgctcttttc acatcctggc
     6841 agtaacgatc tcgacgcgac cgttgtttac ggcgtcaatc aggatcaact tcgtgcggaa
     6901 caccgcatcg tttctaacgc ttcctgtacc acgaattgca taattcccgt catcaaattg
     6961 ttagatgatg cgtacggtat tgagtccggc actgtgacca caattcactc cgccatgcac
     7021 gatcaacagg ttattgatgc ataccatcct gacctgcgtc gcacccgggc agccagccag
     7081 tcgatcattc cggtcgatac taaactggcc gctggtatca cacgattttt tccgcaattt
     7141 aacgatcgct ttgaagcgat tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat
     7201 ttaagcgtga cggtgaagaa acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa
     7261 gcagcacaag gtgcatttca tggtatagtt gactatacgg aattgccgtt ggtctctgta
     7321 gattttaacc acgatccgca cagtgccatt gtcgatggca cccaaacccg ggtcagtggc
     7381 gcacacctga tcaaaacgtt ggtctggtgc gataacgaat ggggctttgc taaccgaatg
     7441 ctcgacacga cgttagctat ggctactgtt gctttcaggt aagacgcaag cagcgtctgc
     7501 aaaactttta gaatcaacga gaggattcac catgtctgta attaagatga ccgatctgga
     7561 tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa
     7621 agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca
     7681 aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg agtacaacga
     7741 agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta acccggttcg
     7801 tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg ttgttctgga
     7861 aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa accctgtcca aaaaatacgc
     7921 tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg cgcaggcttc
     7981 tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga
     8041 actggacgcg ctgggtaaag cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg
     8101 tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa tcgctgacca
     8161 gctgattgtt ggtggtggta tcgctaacac ctttatcgcg gcacaaggcc acgatgtggg
     8221 taaatccctg tacgaagctg acctggttga cgaagctaaa cgtctgctga ccacctgcaa
     8281 catcccggtt ccgtctgatg ttcgcgtagc aaccgagttc tctgaaactg caccggctac
     8341 cctgaaatct gttaacgatg tgaaagctga cgagcagatc ctggatatcg gtgatgcttc
     8401 cgctcaggaa ctggctgaaa tcctgaagaa tgcgaaaacc attctgtgga acggtccggt
     8461 tggcgtgttc gaattcccga acttccgcaa aggtactgaa atcgtggcta acgctatcgc
     8521 agacagcgaa gcgttctcca tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt
     8581 cggcattgct gacaaaatct cctacatctc cactggcggc ggcgcattcc tcgaattcgt
     8641 ggaaggtaaa gtactgcctg cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat
     8701 cacagggcag ggaaacctgc acttgtttca gcgcgctttt agagcacgca ttttttcata
     8761 ctctaaataa ttcgagttgc aggaaggcga caagagagtg aatccccagg agcttacata
     8821 agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag tattatgagt
     8881 ataaggcccg acgatacagg acaagagaca tgtctaagat ttttgatttc gtaaaacctg
     8941 gcgtaatcac tggtgatgac gtacagaaag ttttccaggt agcaaaagaa aacaacttcg
     9001 cactgccagc agtaaactgc gtcggtactg actccatcaa cgccgtactg gaaactgctg
     9061 ctaaagttaa agcgccggtt atcgttcagt tctccaacgg tggtgcttcc tttatcgctg
     9121 gtaaaggcgt gaaatctgac gttccgcagg gtgctgctat cctgggcgcg atctctggtg
     9181 cgcatcacgt tcaccagatg gctgaacatt atggtgttcc ggttatcctg cacactgacc
     9241 actgcgcgaa gaaactgctg ccgtggatcg acggtctgct ggacgcgggt gaaaaacact
     9301 tcgcagctac cggtaagccg ctgttctctt ctcacatgat cgacctgtct gaagaatctc
     9361 tgcaagagaa catcgaaatc tgctctaaat acctggagcg tatgtccaaa atcggcatga
     9421 ctctggaaat cgaactgggt tgcaccggtg gtgaagaaga cggcgtggac aacagccaca
     9481 tggacgcttc tgcactgtac acccagccgg aagacgttga ttacgcatac accgagctga
     9541 gcaaaatcag cccgcgtttc accatcgctg cgtccttcgg taacgtacac ggtgtttaca
     9601 agccgggtaa cgtggttctg actccgacca tcctgcgtga ttctcaggaa tatgtttcta
     9661 agaaacacaa cctgccgcac aacagcctga acttcgtatt ccacggtggt tccggttcta
     9721 ctgctcagga aatcaaagac tccgtaagct acggcgtagt gaaaatgaac atcgataccg
     9781 atacccaatg ggcaacctgg gaaggcgttc tgaactacta caaagcgaac gaagcttatc
     9841 tgcagggtca gctgggtaac ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc
     9901 cgcgcgtatg gctgcgtgcc ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc
     9961 aggaactgaa cgcgatcgac gttctgtaag tcattccttt ctgcttatct caaggcccgc
    10021 tctgcgggtc tttttttcgc caaaagcaag gtgattcaga tgagaaatct gtgatctatt
    10081 tggcaaaatt atgctttatt gtttaccctt gtcagactgc ccgtcataag gcggcggagt
    10141 gtatttctcc attttgagtc agttgaaaag gaatattgaa tggaagattt gaatgttgtc
    10201 gatagcataa acggcgcggg aagctggctg gtagctaacc aggcgctgct gctaagttat
    10261 gcagtaaaca tcgtggcggc actcgcgatc atcatcgttg gtttgattat cgcgcggatg
    10321 atttccaacg cggtgaatcg cctgatgatc tcccgtaaaa tcgatgccac tgttgctgat
    10381 tttctttctg cattagtccg ttacggtatt atcgccttta cgctaatcgc tgccctggga
    10441 cgcgtgggtg tacaaacagc gtcagtcatt gctgtactcg gtgccgcagg cttagctgtt
    10501 ggtctggctt tgcaggggtc actttctaac ctggccgctg gcgtgttact tgtcatgttc
    10561 cgcccgttcc gtgccggaga atatgttgac ctcggcggcg tagccggtac tgtgctgagt
    10621 gtgcagattt tctccaccac catgcgtact gcagacggta aaattatcgt tattccgaac
    10681 ggtaaaatta ttgccggaaa tattattaac ttctcccgcg agccagttcg ccgtaacgaa
    10741 tttattattg gcgtggcgta tgattccgat atcgatcagg ttaagcagat cctgaccaat
    10801 attatccagt ctgaagatcg cattttgaaa gatcgcgaaa tgactgtgcg cctgaacgaa
    10861 cttggtgcat cgtcgattaa tttcgtggtc cgcgtctgga gcaacagcgg cgatctgcaa
    10921 aacgtgtact gggatgtgct ggagcgtatt aaacgtgaat ttgatgccgc cggtatcagc
    10981 ttcccgtacc cgcaaatgga tgtgaacttt aagcgggtga aagaagacaa agctgcgtaa
    11041 tcaacgctga ggccagataa tactctctgg cctctctctt attagttatt ctgattgcca
    11101 attaatatta tcaatttccg ctaataacaa tcccgcgata tagtctctgc atcagatact
    11161 taattcggaa tatccaacgt gttttcttat tactttcaag gtcttgctct tggggcagct
    11221 atgattctgc cgctcggccc acaaaatgct tttgtgatga atcagggcat tcgtcgtcag
    11281 taccacatta tgattgcctt actttgcgct atcagcgatt tggtcctgat ttgcgcgggg
    11341 atttttggtg gtagcgcgtt attgatgcag tcgccgtggt tgctagcgct ggtcacctgg
    11401 ggcggcgtag tcttcttgct ctggtatggt tttggcgctt ttaaaacagc gatgagtagt
    11461 aatattgagt tagctagtgc cgaagtcctg aagcaaggca gatggaaaat tatcgccacc
    11521 atgttggcag tgacctggct gaatccgcat gtttacctgg atacttttgt tgtactgggc
    11581 agccttggcg ggcagcttga cgtcgaacca aaacgttggt ttgcgctcgg gacaattagc
    11641 gcctctttcc tgtggttctt tggtctggct attctcgcag cctggctggc accgcgcctg
    11701 cgcacggcaa aatcacagcg cattatcaat ctggtagtgg gatgtgtcat gtggtttata
    11761 gccttgcagc tggcgagaga cggtattgct catgcacaag ccttgttcag ttaggcgcta
    11821 tctgatggaa aaataaaaca gaggcgctaa gcttgcctcc agaggtcctg aattttgggc
    11881 aagtcaatta acacggagag actaacgtga agttcaaagt tatcgccctg gcggcattaa
    11941 tgggtattag cgggatggca gcgcaggcta acgaattgcc ggatggaccg catattgtca
    12001 cctccggtac ggcaagtgtg gatgcggtgc cagacattgc cactcttgcg attgaagtta
    12061 acgtggccgc gaaggatgcc gctacagcca agaaacaggc agatgagcgc gtcgcacaat
    12121 acatttcctt ccttgaactc aatcagatcg cgaaaaaaga tatcagctca gcgaatttac
    12181 gcacccagcc agattatgac tatcaggatg gtaaaagtat cctgaaaggc taccgagctg
    12241 tgagaacggt ggaagtcacg ctccgtcagt tagacaaact gaattccttg ctggatggcg
    12301 cgctgaaggc gggtcttaac gaaattcgtt ctgtgtcgct gggcgtggcg cagccggatg
    12361 cctataaaga caaagcgcgt aaggcagcga ttgataacgc gattcatcag gcgcaggaac
    12421 tggcgaacgg ctttcatcgt aaactggggc cggtatatag cgtgcgctac catgtttcca
    12481 actatcagcc cagcccaatg gtgcggatga tgaaagccga tgccgcgccg gtgtctgccc
    12541 aggaaactta cgagcaggcc gctattcagt ttgatgatca ggtcgatgtg gtcttccagt
    12601 tagaacctgt ggatcaacaa cccgctaaaa cacctgcagc acaataatac ttgtagcccg
    12661 acgtattcgt cgggctaatg cctttacccg atattgcctc ctttttctgt aattttctgt
    12721 aattttctgt aaaaccatga cgtaatcacc tatctttgct cgcggtttca tcaaggatag
    12781 gctgactgaa acggttaagc ggacaaggat gcggggaggg gggatggaca tttttatttc
    12841 gaaaaagatg cgcaatttca ttttattagc gcaaaccaat aatattgccc gggcggcaga
    12901 aaaaatccat atgacggctt cgccatttgg taaaagcatt gccgcgctgg aagaacaaat
    12961 tggctatacg ctatttaccc gcaaagataa taatatcagc ctcaataaag ccggtcagga
    13021 actgtatcaa aaactgttcc cggtttatca acgactttct gcgattgata atgaaatcca
    13081 taactcaggg cgtcgttcac gagaaattgt gattggcata gataatactt atccgacaat
    13141 tattttcgat cagttgatta gtctcggaga taagtatgaa ggcgtgaccg cccagccagt
    13201 tgagttcagc gaaaacggcg ttatcgataa tctttttgac cgtcagttag attttattat
    13261 ctccccacag catgtatctg cacgagttca ggaactggaa aacctgacta tcagtgaatt
    13321 accaccgctg cgattgggct ttctcgtttc tcgccgttat gaagagcgtc aagagcaaga
    13381 gctgcttcag gagctgccct ggctacaaat gcgctttcaa aaccgggcta acttcgaagc
    13441 aatgattgat gcgaatatgc gaccttgtgg gatcaatccc accattattt atcgtcccta
    13501 cagttttatg gccaaaatca gcgccgtgga gcgcggacat tttctgacgg tgatcccgca
    13561 ttttgcctgg cgactggtga atccggcaac gctgaaatat tttgatgcgc cgcacaggcc
    13621 gatgtatatg caggaatatc tttattcaat cagaaatcat cgttataccg ccacgatctt
    13681 cagcatattg ctgaagatcg tgacgggaca aaccattaac ccagcatcga gccggttgca
    13741 attaaattac ggtgtaagtc gaagacgtgg ctaagatcgt ggtgaatatg tccgccgggc
    13801 gcattttcca gatagcgatg cagataatcc cgatacatag gatgtgcaca attatcaatg
    13861 atagtgcggg cgcgttgaag cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg
    13921 atgatcactt tgacgctgtg ttcgctgtga tcaacatggc tgcacattgg cacgacggtt
    13981 gagatcttgc cttctttagc aatcgacgga gccataaaga tcgacagata cgcgttgcgt
    14041 tcaaaatcac cgctgccgcc gatgccgttc atcagatcga ccccggctac gtgcgttgaa
    14101 tttgcatgcc cgtaaatatc aaactccagg ccgacgttca gagcgatgac gcccagacga
    14161 cggatgattt ccgggttatt ggaaatctcc tgcggacgca acacaatgcg gctggcaaag
    14221 taatccatat tgtcgtaaat cttgcgcagg gaatcggccg agattgtcag gctggaggcg
    14281 ctggccccgc tgattttgcc ggtttccagt aaatgcacca ccgattcctg tagcacttcc
    14341 gaatacatca tgaacggagg aatttccggg ttttccccca gacgtgccat taccgcatta
    14401 ttgatattgc ccacgccact ttgcagcggc agaaattccg gcggaatacg cccatgcgcc
    14461 atttcctgca ataagaacgt gaccacgtta tcggcaatct gctggcacat gggattttgc
    14521 ttatccagca tattaccggc gtcgggcaag ttggtttcca cgacggcgac aatctttttc
    14581 ggatcgattt gcacatagcg ggtaccgacg cgatccattg catggaagat cgacacgcta
    14641 ttgcgccgcg gtggcgcgcc aggaatcaca atatctgcca gttctgcaac gcgcggatcg
    14701 tgatagtgat taagttcaat gatcactttc ttcgctcgca gcagctaggt cggcgcatta
    14761 ccgatcccgc tggttaacca gactcgacca tccggtgcca gtgccgatgc ttcaatgacg
    14821 gcaacatcaa tgtcgccgaa gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc
    14881 aggtcaacga aactcaccgc gccctgattg atctttttac gtaaactgga cgatgtttga
    14941 tatggcgcac gccaggaaac agcatcggcg tcagaaagta catcgtcagc ggcggcgctg
    15001 attgacgccc ccgtcagcag gcgaatttga tacggctttt tggcctcatg ctgttcgtta
    15061 gctctgcggg caatcgcggc gggtagggct ttcggcgaac ccgctggggt aaagccgcta
    15121 aatgccacca tgtcgttatg ctggataatt tctgccgctt catcggcggt catccttgtc
    15181 cactgagttt ccatttttac tccttcgttc gcagggatta atgaccaacg aaattaggtt
    15241 tacgtttttc gaggaaagcg ttcatccctt cctggtaatc ttcgctgtca taaaccgcgc
    15301 ggcgcatccc ctgaatacgt tcaaattcat cggagttcat ggtgtgtgct tcgcccagta
    15361 cacgcagctc ttctttgata acggcaatgg ctaacggcgc tttctcagag atgtggtgcg
    15421 ccatttgtaa ggtgaaatct tccagttctt ccacttccac aacatggttg aggatgccga
    15481 cagccagcgc gcgctgggcg gtgattggcg aagcggtaaa aatcagctct ttgacaatgt
    15541 ggaagcccgc gtcgcgggtc aggttgtgaa tgccgaccag gttatacggg acgccgaggt
    15601 ttacaggcgt cattgagaag gttgaggtac tggcggcgat gatcagatcg gaactcatga
    15661 tcatttcaaa tgcgccaccc caaacactac cttccaccat cgaaatgatc ggtttcggga
    15721 atttttggat catgcgggtg atttgacgca atggatcatc ataggagagc ggatcgcgac
    15781 cgccagacgg cagttcgtga atatcgtgac ctgcggagaa gactttggat ccacttggcg
    15841 cccgcaaaat gatacagcga atttccggcc ggttgagatc gcttaacgcc tgcataagat
    15901 catcaataaa gactttactt aaggcattaa gttttcgtcc atagttaaac tcaatgactg
    15961 ccactttgtt gatagtggca acgttcacat actgataaga cataaaaatt cctttaatca
    16021 aaatattgcg tctggataaa ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta
    16081 ttgtttttga ccgctaaaag cgtctggcgg taatagcgat cgaaatcttc attcgcgaac
    16141 aggtgattca gtacttcttc ttcggtctgc ttacgcagcc attccaccga ttgttgttgc
    16201 cgcacttgtt gtaaacgacc actggcagtt agcgcggttt tgaagtcgat gatggcgtgc
    16261 cagatctcat cgattccacg tttttccagt gcgctacaag tcagaccccg tggctgccat
    16321 tcgtcgtatt tacgtcgcag aatatgcagg gcactctcgt acatatgccg ggcaatggcg
    16381 acattggtat ggttatcgcc atcgtctttg ttgataacga gcagatcagc cacttccatc
    16441 agcccttttt taatgccctg cagatcatcg ccgccaccgg caatttgcaa cgagataaaa
    16501 cagtccacca tgcgggcgac ttccgtttcc gactgcgcga tgccaaccgt ttcgacaatc
    16561 actacgtcat aacccgccgc ttcgcataac agcattaatt cccgtgctcg ctgactggca
    16621 ccgcccagat gaccggagga gggtaccggg cgaataaacg ccgcctcggc acgcgccagg
    16681 tcattcatgc gggttttatc cccgagaatg ctaccgccag tgaccgggct gctgggatcg
    16741 accgcaataa cagcaacctt taatccctca cgaatcaaca acatgccaaa ggcttcaaga
    16801 aaggtacttt tccccgcgcc gggtgtgccg gtaacgccca gtcgcagggc gttaccacag
    16861 aacggcataa tagcgtcaag cagctgtgta cttagcgcct gatgacgtgg gtgacggctt
    16921 tccaccagcg tcatggcttg ggcgagcgtg gcacgctcac cctgacgtaa gcggcgaata
    16981 ctttctgcca gcgtggcttc attaatcatg atgctggctt atcagattca gtacgtcgcg
    17041 cacactgtcg agcataggtg tacctggacc ataaatcgcc gccacgccgc gctcttgcag
    17101 gaaggcgtaa tcctgcggcg gaatgacgcc acccgcgacc acgcagatat cttcgcgtcc
    17161 ccattttttc agcgcttcga ccagttccgg gatcagcgtt ttatgaccgg cagccagtga
    17221 ggatgcgccc actacgtgaa cgtcgttttc tacggctagg cgggcgatct cttcaggtgt
    17281 agagaacatc gggcttaaat ctacgtcgaa accgagatcg gaataggcgc tggcgatcac
    17341 tttcgcgccg cgatcgtgtc catcctggcc catcttagcg atcagaatgc gcgggcgacg
    17401 accattgtcg gcaaggaact gctccgtttg cgcaacaatg gcatcgaact cggaggccga
    17461 tttctcagac tgatgatagc tttgcgcaat cacgccggta acacactggc ttggcaccag
    17521 ataacggtcg aaagcgactt ccagcgcatc ggaaatttca cccagggtgg cgcgaacgcg
    17581 agcggcatta acagcggcag ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc
    17641 gttcaacgcg gcggttacgg cggcatcatc acgggtggcg cgaatgcgtt ccagcgaagc
    17701 aatttgctcg ttacgcacca tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg
    17761 atccagtttg tacttgttga caccaacgat gacacgcttg ccctggtcga tcagcgactg
    17821 ttcgcgcgct gaggcctctt cgatcattcg ttttggcaga cctgcttcga tcgctttcgc
    17881 catgccaccg gcttcgtcga tctgttggat aatagctctg gcttgtttga cgatttgatc
    17941 ggtcagcgac tcaatgtaat aggatccggc cagtggatcg acggtgtggc agagttctga
    18001 ttcttcctgg atgatgatct gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa
    18061 accaagcgct tcgtcaaagg cgttggtatg cagtgactga gtaccgccca gcgtcgcagc
    18121 cagtgcttca atggtggtgc ggataacgtt gttatacgga tcctgttcag tcaggctcca
    18181 gcctgaggtc tggcagtggg tacgcagcgc cagtgatttc ggatcctgtg cgccaaatcc
    18241 actgaccgct tcgctccata aataacgtgc cgcacgcaac atggcgacgt tcataaacag
    18301 atccatgccg ataccgaaga ataacgacag gcgaggagcg aagtcatcaa ttttcagtcc
    18361 ggcagagatt gctgctttga tgtactcaat cccatcggcg agcgtaaatg ctacctgctg
    18421 cacgcagttg gcacccgctt cccccatgtg gtaaccgctg atactgatgg tattaaatcg
    18481 cggcatgttg ccggaacacc aggcgatgat gtcggcgata atgcgcattg acggttttgg
    18541 tgggtaaata taggtgttgc ggcagaggta ctctttgaga atatcgtttt gaatggtgcc
    18601 ggtcagttta tcaggtgtaa caccttgctc ttctgcggcg acgatataaa acgccagtac
    18661 tggtagcact gcgccattca tggtcatcga aaccgacatt ttatccagcg ggatctggtc
    18721 gaacaggact ttcatatctt ccacggtgtc gatagcgacg cccgctttgc cgacgtcacc
    18781 cgccacgcgt gggttatcgg agtcgtagcc acggtgggtg gcaaggtcaa acgcaacgga
    18841 aagacctttt tgcccggcgg ccaggttacg gcgataaaaa gcgttggact cttttgctgt
    18901 tgaaaaacca gcatactgac ggatggtcca cggttgggcg gtatacatag tggcacgcgg
    18961 gccacgaacg tagggcggca aaccaggaag ggtacctgtc acctccagat tatcgagatc
    19021 ggcttcggta tacagcggct tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc
    19081 gacagttttc tcccgacggc tcaattcctt gttggcaagc tgttgccact cctgctcgtt
    19141 agacattatt tgctccgtaa aacgcaatca aaatgagggt atttgcgctt acggtgagct
    19201 ttttacgtgg gtttgtcgcc agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg
    19261 agatttgtct attttgtgat gtgattcaag gaatacaaaa agaagtgagg acaacgcctg
    19321 atatgtgcta tcaggcatta tttgatggat taatcctgac gaaggacttt gtgaccataa
    19381 tcgagtaacg catcagtgac tttacgcatc atgcggcttt caggagcaaa gcggtgccag
    19441 tagagcatcc gtcgttgaaa tagcccaggc gttaagtcaa tcagttcacc gctggccagc
    19501 tctttctcga tttgcaggtg cgggatcata cagcaggtgg tgccctggcg agcaagttgt
    19561 acgaacgctt ctgaagaatt aacgatatgg cagggtacgc tgccaggagg aagatcgaag
    19621 ttttgctgca aaaaggcctg gtgcatatcg tcaagatggt caaacgcgac cactggcgct
    19681 ttcaataatg ccgaacgcgt tacgccgtta ggaaaatatt tttcggcaaa cggttttgag
    19741 ctgacgaaca gatagtcgag cgcaccgagt ttatcgacaa gacaactcgg cagcgcctga
    19801 tgttgaatac tcaccgcgcc gaccacttcg ccgcggcgca gacgttcctg agtacgggtt
    19861 tcatcttcta cctgcaagtt gaggcggata ggcgaatcag ccaacacagg agccagtgcc
    19921 ggaagcaacc acgtcgccag actgtcggcg ttgaccgcca gtgaaagcag cagcggagtc
    19981 gaaccggttt gttcatcgcc cagccactct tcttccagca actccacctg gcgcagcagt
    20041 gccagcagtt tttgcccttg ttccgtcggg cgcggcggta cggtacgcac caacagcggc
    20101 tgcccgaaca tattttccag ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac
    20161 agcttttgtg ccgcgcgctc aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat
    20221 gttctgtagt ccgggcgttt cattgctgtt gtctgctcct ggtaaatgct ttttcctgca
    20281 ctatgacaca attttgtgtc aggttgcata taccgaagcc gtcggcgggt aacatttgta
    20341 tcaatggtta aacgcgcagc ttaaaagatc agggatttgc gctattgcat ttcgcttagc
    20401 gacttcgcgc cctggttatg ttgaatggcg tggcgttatt gcctctgttc gcaccgtgga
    20461 gcggcatacc cagaccgttg tatgctttat ttgcggggcg aaaggggatg cccgccattg
    20521 cgcgatattt tatggatgag ttaaccaccc ggcttgccaa cggggtctga atcgcttttt
    20581 ttgtatataa tgcgtgtgaa atttcatacc acaggcgaaa cgatcatgac gcaggatgaa
    20641 ttgaaaaaag cagtaggatg ggcggcactt cagtatgttc agcccggtac cattgttggt
    20701 gtaggtacag gttccaccgc cgcacacttt attgacgcgc tcggcacgat gaaaggccag
    20761 attgaagggg ccgtttccag ttcagatgct tccactgaaa aactgaaaag cctcggcatt
    20821 cacgtttttg atctcaacga agtcgacagc cttggcatct acgttgatgg cgcagatgaa
    20881 atcaacggcc acatgcaaat gatcaaaggc ggcggcgcgg cgctgacccg tgaaaaaatc
    20941 attgcttcgg ttgcggaaaa atttatctgt attgccgacg cctccaagca ggttgatatt
    21001 ctgggtaaat tcccgctgcc agtagaagtt atcccgatgg cacgtagtgc agtggcgcgc
    21061 cagctggtga aactgggcgg tcgtccggaa taccgtcagg gcgtggtgac cgataatggc
    21121 aacgtgatcc tcgacgtcca cggcatggaa atccttgacc cgatagcgat ggaaaacgcc
    21181 ataaatgcga ttcctggcgt ggtgactgtt ggcttgtttg ctaaccgtgg cgcggacgtt
    21241 gcgctgattg gcacacctga cggtgtcaaa accattgtga aatgatctga cgggggaacc
    21301 tcccccgtta aaaaaattct cttcattaaa tttggtgaca tgtgtcacgc ttttaccagg
    21361 caattgtcga ttgctctaaa taaatcctct aaaccagcat attcatccaa gaattacctt
    21421 tgcgtgatat ttcctcaaca tcgcgacgca aacgttcata ttgccgcaat attatttttt
    21481 gatatgttga aaggcggatg caaatccgca cacaacattt caaaagacag gattgggtaa
    21541 atggcaaagg tatcgctgga gaaagacaag attaagtttc tgctggtaga aggcgtgcac
    21601 caaaaggcgc tggaaagcct tcgtgcagct ggttacacca acatcgaatt tcacaaaggc
    21661 gcgctggatg atgaacaatt aaaagaatcc atccgcgatg cccacttcat cggcctgcga
    21721 tcccgtaccc atctgactga agacgtgatc aacgccgcag aaaaactggt cgctattggc
    21781 tgtttctgta tcggaacaaa ccaggttgat ctggatgcgg cggcaaagcg cgggatcccg
    21841 gtatttaacg caccgttctc aaatacgcgc tctgttgcgg agctggtgat tggcgaactg
    21901 ctgctgctat tgcgcggcgt gccggaagcc aatgctaaag cgcaccgtgg cgtgtggaac
    21961 aaactggcgg cgggttcttt tgaagcgcgc ggcaaaaagc tgggtatcat cggctacggt
    22021 catattggta cgcaattggg cattctggct gaatcgctgg gaatgtatgt ttacttttat
    22081 gatattgaaa acaaactgcc gctgggcaac gccactcagg tacagcatct ttctgacctg
    22141 ctgaatatga gcgatgtggt gagtctgcat gtaccagaga atccgtccac caaaaatatg
    22201 atgggcgcga aagagatttc gctaatgaag cccggctcgc tgctgattaa tgcctcgcgc
    22261 ggtactgtgg tggatattcc ggcgctgtgt gatgcgctgg cgagcaaaca tctggcgggg
    22321 gcggcaatcg acgtattccc gacggaaccg gcgaccaata gcgatccatt tacctctcca
    22381 ctgtgtgaat tcgacaacgt ccttctgacg ccacacattg gcggttcgac tcaggaagcg
    22441 caggagaata tcggcttgga agttgcgggt aaattgatca agtattctga caatggctca
    22501 acgctctctg cggtgaactt cccggaagtc tcgctgccac tgcatggtgg tcgtcgtctg
    22561 atgcacatcc acgaaaaccg tccgggcgtg ctaactgcgc tgaacaaaat cttcgccgag
    22621 cagggcgtca acatcgccgc gcaatatcta caaacttccg cccagatggg ttatgtagtt
    22681 attgatattg aagctgacga agacgttgcc gaaaaagcgc tgcaggcaat gaaagctatt
    22741 ccgggtacca ttcgcgcccg tctgctgtac taattcccct tctctgaaaa ttaacgggca
    22801 ggtcactgac ttgcccgttt ttttatccct tctccacacc gttgccgcgt tgcgcattct
    22861 actttgcgag tctgctccag aaaataaatc aaagtcagat caatgcgttg tgtttattta
    22921 atggggattt acgatggcgg gacagcatgg ggctgtaacg gtaaagccct caccgaagcg
    22981 agggcttaaa ggagaagggt tatgatgcga cttgtcatca tactgattgt actgttactg
    23041 ataagtttcg gagcttatta acagtcagtc tcaagggagg agtcatcctc ccttaccctt
    23101 actcactaaa ttaggtcaaa gaatcaacga tgtcaatcag ggcgatgcgg ggtgcatcgc
    23161 cctgaaccga actttttgat agccacttca tcgcccacgt ttactgccgt aatagatcct
    23221 ttttcccaaa aattatctct gttttgcatg gggtttttcg agcacgctcg aagaaaagat
    23281 ttaaaggtag atcaatgcgt tgtggttatt taaagagagc ttacgatagc tgggcagcat
    23341 ggggctgtaa cggtaaagcc ctcaccgaag cgagggcttg aaggagaagg gttatgatgc
    23401 gacttgccat catactgatt gtactgttac tcataagttt cccagcttat taacagtcag
    23461 tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta aagaatcaac
    23521 gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt tcgacggtgt
    23581 aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca ccaactgaca
    23641 atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta aggtccgatc
    23701 ataaaaacca ccgcccattc ccagacgctg accgtactca tcaaaggcga ccagcggtgt
    23761 gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt ttggctcatg
    23821 gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga acagcaaatt
    23881 accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc gccagagttg
    23941 ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag cgaccgtatg
    24001 cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac ccatttccag
    24061 ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga tttcttgtcg
    24121 ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat aagaagggaa
    24181 tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt gaatgtgtcg
    24241 tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg agcgccacat
    24301 tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc tcagagaaat
    24361 tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa gttttggtta
    24421 gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg ctgcagcatc
    24481 cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc ttgcgctaac
    24541 tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt gactctagtg
    24601 cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt caacgcatcc
    24661 ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat atcgacgggt
    24721 tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt agacccatgg
    24781 tctgcgaagg ggcgacacta tagctaccct gatgagaaga gacaagccct tttctggtcc
    24841 accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac gaatgcttat
    24901 gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga accaacaagg
    24961 gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat gtggcggtaa
    25021 cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca tggctttcgg
    25081 tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg ccctgcagga
    25141 tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg ttttcgatcg
    25201 ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg ttacgcaacc
    25261 gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc gtaacatcgc
    25321 gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc ttgaagagat
    25381 catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc atccgcaacc
    25441 gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt aaggagagtg
    25501 ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg gtggagcaaa
    25561 tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt agcgccgaca
    25621 gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt aacgaaccgg
    25681 aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt ctgtttaacc
    25741 gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag gatgccgcgc
    25801 cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag caactttatc
    25861 aactacttaa cggcctggat gtggtttacc atgcccaggg cgaatatgca tatgctgatg
    25921 aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat ctcaccgcac
    25981 cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc aaatcgccag
    26041 aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat acacgggcga
    26101 tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt caccacgaat
    26161 ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc ggtgaaaacg
    26221 gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac ctggtgttga
    26281 ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc ttcccggtca
    26341 acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag tctctcgaaa
    26401 ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt gaagtggtgc
    26461 gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt gatgaactga
    26521 tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg ttaggactgg
    26581 atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa ccggggatgg
    26641 tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca gaacaatatc
    26701 gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc aacgaaaacc
    26761 tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct gctgcgagaa
    26821 agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg cgctggctat
    26881 ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg cgccagagtc
    26941 acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta cctgtcagca
    27001 actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca tcactaccgt
    27061 gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag attaccaact
    27121 ggcggcgctg gggcaggttg tcgaattgca taatgtcggg caacggctgt ttgcattgct
    27181 gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg ttgcccgtac
    27241 tcagagtcat gttgaagtga cgctggagag cggcgagatg ctgacgggcc gcgtgctgat
    27301 agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt ggcagcagga
    27361 gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc acgaaggccg
    27421 cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt ctgacggacg
    27481 ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt cgtggagcga
    27541 cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc aaattaccca
    27601 cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac cgattaccca
    27661 tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg ggcaagggtt
    27721 taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg cgcatgagcg
    27781 cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc gacagagcga
    27841 tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca accgttgggc
    27901 accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca ccccggcacg
    27961 cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt tttaaggaat
    28021 aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg ctggcggttg
    28081 cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc gtaccggaac
    28141 ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc gccagcgaaa
    28201 aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc agttgttatc
    28261 acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac gatcaaagca
    28321 tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac gcgctgtgga
    28381 acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta cagcaggtcg
    28441 cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta acggcgcgtc
    28501 tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat attccgctga
    28561 ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa gaaccgcatg
    28621 atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta ccgcttagcg
    28681 atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag cggatgcagc
    28741 aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat cgccttggct
    28801 tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat gcgcgccagt
    28861 ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac ccactggcgg
    28921 ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa ctgaaacggt
    28981 tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat gaacgtagcc
    29041 gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat ctcttttccg
    29101 gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc gacacgcttc
    29161 ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg cctgaatggc
    29221 tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc atctcccggt
    29281 tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg ttttcgcatt
    29341 atattttcta ataccatgat tttttgattt agtgtttttt gacatttttt tagctcttaa
    29401 tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa ttggtgtatt
    29461 ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta atgtgatgat
    29521 caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa agagaacgat
    29581 tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt tcaccatgaa
    29641 aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact cctttgtacg
    29701 aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg atgccgctgc
    29761 attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga atgtttgatg
    29821 tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt ctgcgttatc
    29881 tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac tcggggatgt
    29941 tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact gaagatttct
    30001 tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt acccaacacg
    30061 ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt gccgtgcaag
    30121 ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt caggcggtgg
    30181 aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc accactggtt
    30241 ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc gatttctggc
    30301 gtgcgctggt ggaagcgggc gttaagccct gcggtctggg ggcgcgtgac acgctgcgtc
    30361 tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct cctttagccg
    30421 ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc ggtcgtgaag
    30481 ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg atgaccgaaa
    30541 aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc aaccagcatg
    30601 aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt gcgctggcgc
    30661 gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg
    30721 ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga tttacttttt
    30781 tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag aacacgaatg
    30841 gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg ctcaggagct
    30901 gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga
    30961 tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc cagtaagcgg
    31021 tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga acagcgaacc
    31081 gtatgcaggc ggctggatct ttaaaatcaa agccagcgat gaaagcgaac tggaatcact
    31141 gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt attcctcttc
    31201 tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct tcgtaaggag
    31261 agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag acgttaagcc
    31321 agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc gcgcaacagc
    31381 aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc cagattgtgc
    31441 cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc gaatacgccg
    31501 cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct tacatcggca
    31561 tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg gaaaatccgg
    31621 gctggtatac cgcgtacact ccgtatcagc ctgaagtctc ccagggccgc cttgaagcac
    31681 tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc tctgcttctc
    31741 ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac
    31801 tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg ctggatgtgg
    31861 tccgtactcg tgctgaaacc tttggttttg aagtgattgt cgatgacgcg caaaaagtgc
    31921 tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc ggtgaaattc
    31981 acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc agcgttgccg
    32041 ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg gatattgttt
    32101 ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac gcggcattct
    32161 ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt gtatcgaaag
    32221 atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa catattcgtc
    32281 gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac atcgccagcc
    32341 tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc attcaccgtc
    32401 tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc catgcgcact
    32461 atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg cgtgccgaag
    32521 cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc cttgatgaaa
    32581 ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat aaccacgggc
    32641 tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc cagcctgcga
    32701 tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac agcgaaaccg
    32761 aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat caggcgatga
    32821 tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc ccaatcactt
    32881 ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa ggttatcagc
    32941 agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac gccgtttgta
    33001 tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt cgtcattatc
    33061 atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct gcgcacggaa
    33121 ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa
    33181 acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc gataacctct
    33241 cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg atccgtgaag
    33301 tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg aacatgaacg
    33361 cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac ctcaacctgc
    33421 ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg atcggtgtga
    33481 aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa
    33541 cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg ccaatcagct
    33601 ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag gtggcaatcc
    33661 tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg tataccggtc
    33721 gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa gaagaaaccg
    33781 gcatcagcga gctggacatt gccaagcgcc tgatcgacta cggtttccac gcgccgacga
    33841 tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg
    33901 aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac caggtgaaag
    33961 ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt cagagcgaac
    34021 tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg gcaggcgtgg
    34081 cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac cgtaacctgt
    34141 tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt attttctaaa
    34201 ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga cagtcccctc
    34261 gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc gaatatttac
    34321 cctcaccccg accctctccc tgaaagggcg agggggaaaa gcgtgccaac attgaagatt
    34381 gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct cggaaaaaca
    34441 aatggctata gcacttgtga ccggtggcag tcgcggcatc gggcgggcaa ctgcattact
    34501 gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc acgcggcgca
    34561 ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc aggcggatat
    34621 cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg atgaaccgct
    34681 agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg aaaaccttac
    34741 cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc tctgctgccg
    34801 cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta tcgtcaatgt
    34861 ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg cggcatcgaa
    34921 aggggcgatt gattcgttaa ccaccggact atcgctggaa gtcgccgcgc aggggatccg
    34981 cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg gcggcgagcc
    35041 tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac aggcagaaga
    35101 ggtcgcgcag gccattgtct ggctactaag tgataaagcc tcttacgtca cgggaagttt
    35161 tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg caacaagtat
    35221 tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca cctctttgta
    35281 ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt catgtttatt
    35341 cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca cgcagtcgat
    35401 gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg cttttttcat
    35461 ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc tgccatcttc
    35521 ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc tctgataacg
    35581 ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc agccccagtc
    35641 agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg catcgccggt
    35701 gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat aatcgcaggt
    35761 gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc cgcgacgttc
    35821 ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg taaagacgta
    35881 gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg agtagggata
    35941 gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc gacgcgcggc
    36001 tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca tcgtctcttc
    36061 cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca gcggcgcacg
    36121 ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt tatgcttgta
    36181 gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac ggttggtcca
    36241 gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg ttatcaccgg
    36301 ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca gcccttcttc
    36361 gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg aagtccgaaa
    36421 acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac cataaaaatc
    36481 aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga tttcgcgcgg
    36541 cacgccgtgt gcgccacagg tcagaacgtc acaaatgctc ggtcctttgc cgcctttgtt
    36601 ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat ctttcggtaa
    36661 ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga aatagtacca
    36721 tgcagccaaa cgacatcact tttttcaacg tttccaggat gacattctgg ctgggcgtaa
    36781 aaccatcacc atccgcgacg agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt
    36841 cggacgtttt gaagatgacg gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt
    36901 aacgctggat acactgacgg aaaaacatgc agagcaggaa aatatgaccc tgcctgaact
    36961 gaaaaaggtc attgctgaca tctatcccgg tcagacacaa ttttatatga ttgaatttaa
    37021 atgtctttaa attgaactca cgcctgttag ttgaaatttc gcatttaatt tcatgatttt
    37081 taggagatta gtctcaaatc agaatttatt ttagctaaca ggtgttcact ggaactattc
    37141 tcagttacgc tggagaggta tacatgtgcg tgtacgtttc tctggagtaa gttatggttc
    37201 agaagcccct cattaagcag ggatattcgc tggcagagga aattgccaac agcgtcagtc
    37261 acggcattgg gctggtgttt ggtatcgttg ggctggtgtt gctactggtt caggcggtgg
    37321 atctcaatgc cagcgccaca gcgataacca gttacagcct ctatggcggc agtatgatcc
    37381 tgctgttcct cgcttcgacg ctctatcacg ccattcctca tcaacgggca aaaatgtggc
    37441 tgaagaaatt tgaccattgc gctatttacc tgttgattgc cggaacctac acgccgtttt
    37501 tgctggtggg gctggattct ccgttagcgc gcgggttgat gattgttatc tggagcctag
    37561 cattgctggg gattctgttc aaactgacca tcgcgcaccg attcaaaatt ttatctctgg
    37621 tgacttatct ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc
    37681 tcgcggcggg cagcgttacc ttactggcgg taggcggcgt ggtttattcg ctcggggtga
    37741 ttttctacgt ctgcaaacgc attccataca accatgccat ctggcacggc ttcgtgctcg
    37801 gcggtagtgt gtgccacttt ctggcgatct atttgtatat tgggcaggcg taatgggtat
    37861 gcctttgtag gcctgataag acgcgtcagc gtcgcatcag gcatcggagc gcaaccgccg
    37921 gatgcggcgt gaacgcctta tccgatctac gggcctcgtc cctgttgtga taaaggcatc
    37981 aaatcccaga catgataaga cgcgtcagcg tcgcatcagg cgataaatgc ctgatgcgat
    38041 acagagattt actcttcgag cgaatacggc agcggctcga tatgcagtgt attcgcatcg
    38101 tcgcgtacgc ggaagatgct gtccggttcc atatcgttat tcatgacgac ctgtaccacg
    38161 acctgaccat cttccagttt taccgcagcc agcaccgtac cggtacgacg ccagttctcg
    38221 cccattttca gctctaagtc ttcaccagct tccggcagtc ggctggcact acctgccagc
    38281 aaccagagcg cgcgtttgtt ggcaccacgg aattttgctc gcgccaccat ctcttgtccg
    38341 gtataacagc ctttcttaaa gctgataccg cccagcgcct ggagattggt cgcctgtggg
    38401 ataaactgcc cgctgttggc ggcatcaatc accgggaaac cagcttcaat gtttaatgcc
    38461 agccactgtt ggctattgtt cagttccgct tcaccgcgca gtttatcggt cagcatatta
    38521 gcagtagctt catcggttac gatcaggaaa cgttctgccg ggtgttcaaa ccatagcaaa
    38581 gtggtcgcgc cttctttgac tacctgtttt tctttcgaag gcagttcgct aaagagattt
    38641 gccagcgcgg cgcgcgcctg aaaaccggca acaccaagca gcacacgctc gtcgtctggc
    38701 gcgatggtca ctttagagaa taccgcatat tttttcagtt cagtcagctg cggttcacgc
    38761 acgctgcgcc gttcaatcca tgcaaagcca tcgccgtcgc ggaacagacg taaattgctc
    38821 cacattttac ctttggcgtc gcaatgggcg gcgagcaggt gctgatcttc tgccatctgg
    38881 ctgacatctg ctgtcacctg accctgcata tatttttcgc tgtccgcgcc agtaatggtg
    38941 gcaagcgccc agtcatcaag cgtcatcagc gtcagcggta aacgggcaga agccgtaggc
    39001 tgacgtggag gaaaaggtgt aaaagccata atagagtcct gagttgctta acggcgtatt
    39061 aatgactaat ggtaaaagag ctatgaggca atgcaagtgc tttcactacg cgatctgtgc
    39121 cttctgcgag gcgactgcca aaaaaatgaa gcccttgaat tgttgtgaat taatttcgcg
    39181 atcgcgatgg tgtttactga tattcccgta aaagaagtcg aaaaacacgt tacaatagcc
    39241 gggtactgga ttttcgtgag acaggaagaa gatggacatt aacaacaaag cccgaattca
    39301 ttgggcatgc cgccgtggta tgcgcgaact cgatatttca atcatgtcgt ttttcgaaca
    39361 tgagtacgac agcttaagcg atgacgaaaa acgcatcttt attcgtctgc tggaatgtga
    39421 cgatccggac ctgtttaact ggctgatgaa tcacggtaaa ccggccgatg cagaactgga
    39481 aataatggtc cgactcatcc agacacggaa ccgggaacgt ggtcctgtgg caatctgatt
    39541 tgcgcgtctc ctggcgcgca cagtggcttt ccttgctgat tcatgggctg gttgccgctg
    39601 ttattttact catgccctgg ccgctcagtt acaccccgtt atggatggtg ttactttcgc
    39661 tggtggtatt tgattgcgtt cgcagccagc ggtgtattaa tgctcgccag ggggaaattc
    39721 gcttgttgat ggacgggcgt ttgcgttggc aagggcagga gtggagcatc gtcaaagcac
    39781 cgtggatgat taagagcggc atgatgctgc gtttacgttc tgatggcggt aaacggcaac
    39841 atttatggct ggcagccgac agcatggacg aagccgaatg gcgggattta cggcggattt
    39901 tgttgcaaca agagacgcaa agataaagat gacggcaaag catatgcgct aagccgtcag
    39961 tgagatcagg cgtaatgctc tgccatttcg ttgaggattt gctcgcacca gctctgaata
    40021 cgctcgtcgc taaggtcata ctggttagtt tcatccagcg ccagacccac gaacagttgc
    40081 ccgtcagcaa tcaccggttt cgggctggta aattcatatc cttccgttgg ccagtagccg
    40141 acgaacttca cgcctttggt cgagagtttg tcatgcagca taccgagcgc atcgaggaac
    40201 cactcgccgt atcccagttg atcgccaagc ccatacagcg caataatttt accttcaagg
    40261 ttcaggtcgt cgagctgatc ccagacggct tcccagtctt cctggatttc accaaaatcc
    40321 caggtcggga tacccagaat gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc
    40381 ttgaggttat gtaaggtcac cagttctggg ccgataatat cgcggatttt ttctgccgcc
    40441 atttcggtgt aacaggtgct ggaaccgtaa aaaagaccca tattcataac gtaagcttct
    40501 cgattctgct gtggcttttg cagctagtgt atcagaatcg ctttagtcac ggcacaataa
    40561 ggcataatgg atcaaatgag tgaccataag gggcgcaagt gaaacaggag ctggcacgca
    40621 tcgagcagtt tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg
    40681 cttaccgtcg cgatctgtca atgatggtgg agtggttgca tcaccgcggg ttgacgctgg
    40741 cgacggcgca aagtgacgat ttgcaggcat tactggcaga acggctggag ggcgggtata
    40801 aagccaccag ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag tatctttatc
    40861 gcgaaaagtt tcgtgaagac gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc
    40921 gtttgccaaa agatttaagc gaagcgcagg tcgaacgttt attacaggcg ccattaattg
    40981 atcagccact ggagctacgc gataaagcca tgcttgaagt gttgtatgct accgggctgc
    41041 gtgtctctga actggtcggg ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc
    41101 gggtcattgg taaaggcaac aaagagcgcc tggtgccgtt aggggaagag gcggtttact
    41161 ggctggaaac ctatctggaa catgggcgtc cgtggctgct gaatggtgtg tcaattgacg
    41221 tgttgtttcc cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg caccgcatta
    41281 aacattatgc cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc
    41341 acgcttttgc cactcattta ttaaatcatg gtgcggattt acgcgtggtg cagatgctac
    41401 tgggccacag cgatctttcc accacgcaaa tttatacgca tgtcgctacc gagcgtctgc
    41461 ggcaacttca tcaacagcat cacccgcggg cgtgatgtct gaaaagaacg ggaagattta
    41521 tgaagaaagg ttttatgttg tttactttgt tagcggcgtt ttcaggcttt gctcaggctg
    41581 atgacgcggc aattcaacaa acgttagcca aaatgggcat caaaagcagc gatattcagc
    41641 ccgcgcccgt agctggcatg aagacagttc tgactaacag cggtgtgttg tacatcaccg
    41701 atgatggtaa acatatcatt caggggccaa tgtatgacgt tagtggcacg gctccggtca
    41761 atgtcaccaa taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt
    41821 ataaagcgcc gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact
    41881 gccacaaact gcatgagcaa atggcagact ataacgcgct ggggatcacc gtgcgttatc
    41941 ttgctttccc gcgccagggg ctggacagcg atgcagagaa agaaatgaaa gctatctggt
    42001 gtgcgaaaga taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag
    42061 ccagctgcga cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg
    42121 gtactccggc agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag
    42181 acatgaaaga attcctcgac gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa
    42241 acaacagata caacttcgtc gccgtgaagt cgatgaaacg gcagacttgc ccgctgaatt
    42301 gcctcccttg ctgcgccgtt tatacgccag ccggggagta cgcagtgcgc aagaactgga
    42361 acgcagtgtt aaaggtatgt tgccctggca gcaactaagc ggtgtcgaaa aggccgttga
    42421 gatcctttac aacgcttttc gcgaaggaac gcggattatt gtggtcggtg atttcgacgc
    42481 cgacggcgcg accagcacgg ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa
    42541 tatcgactac ctggtaccaa accgtttcga agacggttac ggcttaagcc cggaagtggt
    42601 cgatcaggcc catgcccgtg gcgcgcagtt aattgtcacg gtggataacg gtatttcctc
    42661 ccatgcggga gttgagcacg ctcgctcgtt gggcatcccg gttattgtta ccgatcacca
    42721 tttgccgggc gacacattac ccgcagcgga agcgatcatt aaccctaact tgcgcgactg
    42781 taatttcccg tcgaaatcac tggcaggcgt gggtgtggcg ttttatctga tgctggcgct
    42841 gcgcaccttt ttgcgcgatc agggctggtt tgatgagcgc ggcatcgcaa ttcctaacct
    42901 ggcagaactg ctggatctgg tagcactggg gacagtagcg gacgtcgtgc cgctggacgc
    42961 taataatcgc attctgacct ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc
    43021 agggattaaa gcgctgctgg aagtggcaaa tcgcgatgca caaaaactag ccgccagcga
    43081 tttaggtttt gcgctgggac cgcgtctcaa tgctgccggg cgactggatg atatgtccgt
    43141 tggtgtggcg ctgttgctgt gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact
    43201 cgatgcgcta aaccagacgc gaaaagagat cgaacaagga atgcaggttg aagccctgac
    43261 cctgtgcgag aaactggagc gcagccgtga cacgctaccc ggcgggctgg caatgtatca
    43321 ccccgaatgg catcagggcg ttgtcggtat tctggcttcg cgtatcaaag agcgttttca
    43381 ccgtccggtt atcgcctttg cgccagcagg tgacggtacg ctgaaaggtt ccggtcgctc
    43441 cattcagggg ctgcatatgc gtgatgcgct ggagcgatta gacacactct accccggcat
    43501 gatgctcaag tttggcggtc atgcgatggc ggcgggtttg tcgctggaag aggataaatt
    43561 cgaactcttt caacaacggt ttggcgaact ggttactgag tggctggccc cttcgctatt
    43621 gcaaggcgaa gtggtgtcag acggtccgtt aagcccggcc gaaatgacca tggaagtggc
    43681 gcagctgctg cgcgatgctg gcccgtgggg gcagatgttc ccggagccgc tgtttgacgg
    43741 tcatttccgt ctgctgcaac agcggctggt gggcgaacgt catttgaagg tgatggtcga
    43801 accggtcggc ggcggtccac tgctggatgg tattgctttt aatgtcgata ccgctctctg
    43861 gccggataac ggcgtgcgcg aagtgcaact ggcttataag ctcgatatca acgagtttcg
    43921 cggcaaccgc agcctgcaaa ttatcatcga caatatctgg ccaatttagc gtcatcttct
    43981 ctataaaaaa gagcgtggat tgggtacaat cccgctctta tcaccgcatt ttgactagct
    44041 caataaaaga aatcagacca tgtttgaaat taatccggta aataatcgca ttcaggacct
    44101 cacggaacgc tccgacgttc ttagggggta tctttgacta cgacgccaag aaagagcgtc
    44161 tggaagaagt aaacgccgag ctggaacagc cggatgtctg gaacgaaccc gaacgcgcac
    44221 aggcgctggg taaagagcgt tcctccctcg aagccgttgt cgacaccctc gaccaaatga
    44281 aacaggggct ggaagatgtt tctggtctgc tggaactggc tgtagaagct gacgacgaag
    44341 aaacctttaa cgaagccgtt gctgaactcg acgccctgga agaaaaactg gcgcagcttg
    44401 agttccgccg tatgttctct ggcgaatatg acagcgccga ctgttacctc gatattcagg
    44461 cggggtctgg cggtacggaa gcacaggact gggcgagcat gcttgagcgt atgtatctgc
    44521 gctgggcaga atcgcgtggt ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg
    44581 cgggtattaa atccgtgacg atcaaaatct ccggcgatta cgcttacggc tggctgcgta
    44641 cagaaactgg cgttcaccgc ctggtgcgta aaagcccgtt tgactccggc ggtcgtcgcc
    44701 acacgtcgtt cagctctgcg tttgtttatc cggaagttga tgatgatatt gatatcgaaa
    44761 tcaacccggc ggatctgcgc attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg
    44821 ttaaccgtac cgaatctgcg gtgcgtatta cccacatccc gaccgggatc gtgacccagt
    44881 gccagaacga ccgttcccag cacaagaaca aagatcaggc catgaagcag atgaaagcga
    44941 agctttatga actggagatg cagaagaaaa atgccgagaa acaggcgatg gaagataaca
    45001 aatctgacat cggctggggc agccagattc gttcttatgt ccttgatgac tcccgcatta
    45061 aagatctgcg caccggggta gaaacccgca acacgcaggc cgtgctggac ggcagcctgg
    45121 atcaatttat cgaagcaagt ttgaaagcag ggttatgagg aaccaacatg tctgaacaac
    45181 acgcacaggg cgctgacgcg gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga
    45241 agctggcgaa cctgcgtgag cagggaattg ccttcccgaa cgatttccgt cgcgatcata
    45301 cctctgacca attgcacgca gaattcgacg gtaaagagaa cgaagaactg gaagcgctga
    45361 acatcgaagt cgccgttgct ggccgcatga tgacccgccg tattatgggt aaagcgtctt
    45421 tcgttaccct gcaggatgtg ggtggccgca ttcagctgta cgttgcccgt gacgatctgc
    45481 cggaaggcgt ttacaacgag cagttcaaaa aatgggacct cggcgacatc ctcggcgcga
    45541 aaggtaaact gttcaaaacc aaaaccggcg aactgtctat ccactgtacc gagctgcgtc
    45601 tgctgaccaa agcactgcgt ccgctgccgg ataaattcca cggcttgcag gatcaggaag
    45661 cgcgctatcg tcagcgttat ctggatctca tctctaacga tgaatcccgt aacaccttta
    45721 aagtacgctc gcagatcctc tctggtattc gccagttcat ggtgaaccgc ggctttatgg
    45781 aagttgaaac gccgatgatg caggtgatcc caggcggtgc cgctgcgcgt ccgtttatca
    45841 cccaccataa cgcgctggat ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca
    45901 agcgtctggt ggtcggtggc ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg
    45961 aaggtatttc cgtacgtcat aacccagagt tcaccatgat ggaactctac atggcttacg
    46021 cagattacaa agatctgatc gagctgaccg aatcgctgtt ccgtactctg gcacaggata
    46081 ttctcggtaa gacggaagtg acctacggcg acgtgacgct ggacttcggt aaaccgttcg
    46141 aaaaactgac catgcgtgaa gcgatcaaga aatatcgccc ggaaaccgac atggcggatc
    46201 tggataactt cgactctgcg aaagcgattg ctgaatctat cggtatccac gttgagaaga
    46261 gctggggtct gggccgtatc gttaccgaga tcttcgaaga agtggcagaa gcacatctga
    46321 ttcagccgac cttcattact gaatatccgg cagaagtttc tccgctggcg cgtcgtaacg
    46381 acattaaccc ggaaatcaca gaccgctttg agttcttcat cggtggtcgt gaaatcggta
    46441 acggctttag cgagctgaat gacgcggaag atcaggcgca acgcttcctg gatcaggttg
    46501 ccgcgaaaga cgcaggtgac gacgaagcga tgttctacga tgaagattac gttaccgcac
    46561 tggaacatgg cttaccgccg acagcaggtc tggggattgg catcgaccgt atggtaatgc
    46621 tgttcaccaa cagccatacc atccgcgacg ttattctgtt cccggcgatg cgtccggtga
    46681 aataagcatt acgttatgct cacaaccccg gcaaatgtcg gggtttttta tttaagctgg
    46741 gtaaatgcag ataatcgtat tctggcttcg cgatttgtcg cctgcatcac catccacgga
    46801 ctgaacgccc acggcgtggc atcaataccg cgtaatacat cagctaaatc acaccattga
    46861 taatccatca cttcatcatc gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc
    46921 ggacacactt cattttccac aatgcctctc ggatcggtgg cgcggtagcg aaagtcagga
    46981 tagatagatt caggaggcgt aatttccacg ccaagctcat aacggcaacg gcggatcacc
    47041 gcgtcttcgc tgctttctcc cagttgtggg tgcccacaaa ccgagttagt ccacacgcca
    47101 ggccacgctt ttttgctcag tgcgcggcgg gtaactaata attgcccctt ggcattaaac
    47161 agccaactgg agaacgcgag atgtaagagg gtgtctgccg tgtgtgcggc atacttttcc
    47221 agcgtacccg tgggaactcc ctgtgcattc aataaaatga cgtgttccgt ttgcataatt
    47281 tctcacatgt aattctgatc actcatcatt atagataatt gaagagtgaa atgattaacc
    47341 aagagagcgt ttatcgacaa ccgcccgttt tgagcggatg ccgacaggta aaaaccattt
    47401 aaaaaagtag catcatatat ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa
    47461 tgacaggaat agagagagta aaaagcaaca acagactgaa tatagcgcgc attaaaaaat
    47521 tcatattatt ctccatgctc atttttcttc agcttgcgca tcagaagcca catacgtact
    47581 gtcctgactt ctaaatcttt atcagagacg gattccttca ccaggtcagc atgatgctga
    47641 tagccattaa agaagacgtt cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta
    47701 tgtagcagcg gttgcagtac ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga
    47761 gtcatgccaa cgccgagact gatcgccaca atatagaggt tgtaacggtt ggtggtgtag
    47821 ttacagcgcg acagaattcg aatccctgtc gccagtacca tgccgaacat cactagacca
    47881 gcgccgccca gcacaaattg cggaatggag gctaccagca ccgccatttt tggcaccatg
    47941 ccaaacagga ttaatataat tcccgaagaa atacacaccc agcggctatg aacgcgcatc
    48001 acgctaacca ggccaacgtt ttgagaaaat gacgtgtggg ggaagctgtt aaacgtgccg
    48061 cctatcattg tccctacgcc atcgacacgc agcccgcaaa taatatcgtg tgaagagagt
    48121 ttacgaccga ctatttcacc cagtgccagg aacatcccca tcgactcgat aaacacgatg
    48181 attaacacgg cagtcatggt cagaatggaa acgggatcga aaatcggcat accaaacgac
    48241 atcggggtga caatcgcaaa ccacgaggca tcatgtagcc cggataaatt gacttcattc
    48301 atcatccacg aaagtaaaaa gccaaataca atccccagta atacggcgac gttggacata
    48361 aatcctttcg catagcgagt aatgagcaag ataaaaatta agacggcaaa ggagatacct
    48421 aaataaacgg gattaccata ttgcggattc cctttacctc cagcggccca gtcaataccc
    48481 acctgaatga tgctaagccc aatagaagta ataaccacac cggtaaccag tggcgggaat
    48541 aaaggcatca agcgaccaat aagtggcgct aataatgtgg tgataaaacc cgcggcgata
    48601 gtggcaccaa atatccccag caggccgata tccgggttca tcccaatggc tatcattggt
    48661 gttacagcag caaaggtcac cgacataatc accggcaggc ggatccccat aaagcggccg
    48721 ataccgatac attgcaataa tgtgacgatc ccgcagcaaa agagatccga gctaatgagc
    48781 atcgcaatag catcttttga gaggcccagt cggtcaccaa tcataagagg aacagcgact
    48841 gcacctgcgt acatgacaag gacgtgttgc agaccgagta tgatcagctt tcctggtgat
    48901 aatatgcggt caacctcatc agttgggcgg tcttgcccag aagatgaggg aagttgggaa
    48961 tctatggcgc tcatcagtgt ttctcctttc gcccgccaga ataaaaagca accgtttacg
    49021 ttaatgaaac tgtctaaaca catgctcgtg gagtgccagt ttttgcgagg aaggggagtt
    49081 agagacagga attgcggagg caatcacata atcgtgctta aaccacgaaa atgacaagag
    49141 aaactgacaa tggaattatc tgtaattttc tcatattgat atttcattaa tcattctggc
    49201 atgtcgcact ctcgcattta a
//
LOCUS       DAJTRM010000019        48002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRM010000019 DAJTRM010000000
VERSION     DAJTRM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.019"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(196..1386)
                     /gene="nepI"
                     /locus_tag="OO322_001187"
     CDS             complement(196..1386)
                     /gene="nepI"
                     /locus_tag="OO322_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5906142.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1597..2049)
                     /locus_tag="OO322_001188"
     CDS             complement(1597..2049)
                     /locus_tag="OO322_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5906143.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2102..3436)
                     /gene="adeQ"
                     /locus_tag="OO322_001189"
                     /gene_synonym="yicO"
     CDS             complement(2102..3436)
                     /gene="adeQ"
                     /locus_tag="OO322_001189"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5906144.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3611..5377
                     /gene="adeD"
                     /locus_tag="OO322_001190"
     CDS             3611..5377
                     /gene="adeD"
                     /locus_tag="OO322_001190"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5906145.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5423..6814)
                     /gene="uhpT"
                     /locus_tag="OO322_001191"
     CDS             complement(5423..6814)
                     /gene="uhpT"
                     /locus_tag="OO322_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5906146.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(6952..8271)
                     /gene="uhpC"
                     /locus_tag="OO322_001192"
     CDS             complement(6952..8271)
                     /gene="uhpC"
                     /locus_tag="OO322_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5906147.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8281..9783)
                     /gene="uhpB"
                     /locus_tag="OO322_001193"
     CDS             complement(8281..9783)
                     /gene="uhpB"
                     /locus_tag="OO322_001193"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5906148.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9783..10373)
                     /gene="uhpA"
                     /locus_tag="OO322_001194"
     CDS             complement(9783..10373)
                     /gene="uhpA"
                     /locus_tag="OO322_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5906149.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10448..10738)
                     /gene="ilvN"
                     /locus_tag="OO322_001195"
     CDS             complement(10448..10738)
                     /gene="ilvN"
                     /locus_tag="OO322_001195"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5906150.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10742..12430)
                     /gene="ilvB"
                     /locus_tag="OO322_001196"
     CDS             complement(10742..12430)
                     /gene="ilvB"
                     /locus_tag="OO322_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5906151.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12536..12634)
                     /gene="ivbL"
                     /locus_tag="OO322_001197"
     CDS             complement(12536..12634)
                     /gene="ivbL"
                     /locus_tag="OO322_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5906152.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13199..13288
                     /gene="tisB"
                     /locus_tag="OO322_001198"
     CDS             13199..13288
                     /gene="tisB"
                     /locus_tag="OO322_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5906153.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13568..14757
                     /gene="emrD"
                     /locus_tag="OO322_001199"
                     /pseudo
     CDS             13568..14757
                     /gene="emrD"
                     /locus_tag="OO322_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
     gene            complement(14765..15262)
                     /locus_tag="OO322_001200"
     CDS             complement(14765..15262)
                     /locus_tag="OO322_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5906154.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15259..15621)
                     /locus_tag="OO322_001201"
     CDS             complement(15259..15621)
                     /locus_tag="OO322_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5906155.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15611..15958)
                     /locus_tag="OO322_001202"
     CDS             complement(15611..15958)
                     /locus_tag="OO322_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5906156.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16067..16480
                     /locus_tag="OO322_001203"
     CDS             16067..16480
                     /locus_tag="OO322_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906157.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16527..17969)
                     /locus_tag="OO322_001204"
                     /pseudo
     CDS             complement(16527..17969)
                     /locus_tag="OO322_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18004..19719)
                     /locus_tag="OO322_001205"
     CDS             complement(18004..19719)
                     /locus_tag="OO322_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5906158.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            19886..20779
                     /locus_tag="OO322_001206"
     CDS             19886..20779
                     /locus_tag="OO322_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5906159.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20776..21590)
                     /locus_tag="OO322_001207"
                     /pseudo
     CDS             complement(20776..21590)
                     /locus_tag="OO322_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21590..23206)
                     /locus_tag="OO322_001208"
     CDS             complement(21590..23206)
                     /locus_tag="OO322_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5906160.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23501..24175
                     /locus_tag="OO322_001209"
     CDS             23501..24175
                     /locus_tag="OO322_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5906161.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24214..25875)
                     /locus_tag="OO322_001210"
     CDS             complement(24214..25875)
                     /locus_tag="OO322_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5906162.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26071..26499)
                     /gene="ibpB"
                     /locus_tag="OO322_001211"
     CDS             complement(26071..26499)
                     /gene="ibpB"
                     /locus_tag="OO322_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5906163.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26611..27024)
                     /gene="ibpA"
                     /locus_tag="OO322_001212"
     CDS             complement(26611..27024)
                     /gene="ibpA"
                     /locus_tag="OO322_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5906164.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27018..27090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27329..27661
                     /locus_tag="OO322_001213"
     CDS             27329..27661
                     /locus_tag="OO322_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5906165.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27663..28895)
                     /locus_tag="OO322_001214"
     CDS             complement(27663..28895)
                     /locus_tag="OO322_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5906166.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            28996..30096
                     /gene="cbrA"
                     /locus_tag="OO322_001215"
     CDS             28996..30096
                     /gene="cbrA"
                     /locus_tag="OO322_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5906167.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30057..31394)
                     /locus_tag="OO322_001216"
     CDS             complement(30057..31394)
                     /locus_tag="OO322_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5906168.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31469..32617)
                     /gene="dgoD"
                     /locus_tag="OO322_001217"
                     /pseudo
     CDS             complement(31469..32617)
                     /gene="dgoD"
                     /locus_tag="OO322_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32614..33231)
                     /gene="dgoA"
                     /locus_tag="OO322_001218"
     CDS             complement(32614..33231)
                     /gene="dgoA"
                     /locus_tag="OO322_001218"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5906169.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33215..34093)
                     /gene="dgoK"
                     /locus_tag="OO322_001219"
     CDS             complement(33215..34093)
                     /gene="dgoK"
                     /locus_tag="OO322_001219"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5906170.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34090..34779)
                     /gene="dgoR"
                     /locus_tag="OO322_001220"
     CDS             complement(34090..34779)
                     /gene="dgoR"
                     /locus_tag="OO322_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5906171.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35057..35713
                     /locus_tag="OO322_001221"
     CDS             35057..35713
                     /locus_tag="OO322_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906172.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35759..36571)
                     /gene="yidA"
                     /locus_tag="OO322_001222"
     CDS             complement(35759..36571)
                     /gene="yidA"
                     /locus_tag="OO322_001222"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5906173.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36685..37083)
                     /locus_tag="OO322_001223"
     CDS             complement(36685..37083)
                     /locus_tag="OO322_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5906174.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37323..39737)
                     /gene="gyrB"
                     /locus_tag="OO322_001224"
     CDS             complement(37323..39737)
                     /gene="gyrB"
                     /locus_tag="OO322_001224"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5906175.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39766..40839)
                     /gene="recF"
                     /locus_tag="OO322_001225"
     CDS             complement(39766..40839)
                     /gene="recF"
                     /locus_tag="OO322_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5906176.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(40839..41939)
                     /gene="dnaN"
                     /locus_tag="OO322_001226"
     CDS             complement(40839..41939)
                     /gene="dnaN"
                     /locus_tag="OO322_001226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5906177.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(41944..43347)
                     /gene="dnaA"
                     /locus_tag="OO322_001227"
     CDS             complement(41944..43347)
                     /gene="dnaA"
                     /locus_tag="OO322_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5906178.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43644..43724
                     /locus_tag="OO322_001228"
     CDS             43644..43724
                     /locus_tag="OO322_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906179.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            43954..44094
                     /gene="rpmH"
                     /locus_tag="OO322_001229"
     CDS             43954..44094
                     /gene="rpmH"
                     /locus_tag="OO322_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5906180.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44111..44470
                     /gene="rnpA"
                     /locus_tag="OO322_001230"
     CDS             44111..44470
                     /gene="rnpA"
                     /locus_tag="OO322_001230"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5906181.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44434..44691
                     /gene="yidD"
                     /locus_tag="OO322_001231"
     CDS             44434..44691
                     /gene="yidD"
                     /locus_tag="OO322_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5906182.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44694..46340
                     /gene="yidC"
                     /locus_tag="OO322_001232"
     CDS             44694..46340
                     /gene="yidC"
                     /locus_tag="OO322_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5906183.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46446..47810
                     /gene="mnmE"
                     /locus_tag="OO322_001233"
     CDS             46446..47810
                     /gene="mnmE"
                     /locus_tag="OO322_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5906184.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11873 a        12996 c        12156 g        10977 t
ORIGIN      
        1 cacatgcgtc attatcagat gaagcgtggg aaaagttaat gttgtcagat gaaaacaata
       61 aacaacatat acgcgaggcc atcgtggcga tggaacgtaa taatcagagc aattattggg
      121 aagcgttggg taaggtagaa tgcccggata tgtaagaaca gcaacaacta ctgaccaacg
      181 cttctttacc tgatatcagg atttcttcat tttcaccttt gcagtaacca acaatgcagt
      241 cagcaacatc aatgtgccgg acaacatcaa cggcgaagtc agaccaatat tatccagcgc
      301 ataaccgccg attgccgcgc cacaggtatt agcaagctga ataaccgcca cctgaataga
      361 cccggctttt tctgcctgat cggccagcga gcgggtgatc cacgttgacc agccgacggg
      421 aaccaatgca aaagttagcc cccagataat cgccacgccg gtagcaacga ttttatcgct
      481 tccccacaac gtcagtacca acgcactcac agccagtatt aacggcgcgc ctgctaaggc
      541 cagttttacc gaacgtttaa gaatgaacga cgaaagcgac gtaccaataa agctggcgat
      601 accaaaactc aacagcacca gcgttaagcc atccacgccg aatcccgcca ggttcatata
      661 caccgggcga atatacgtga agaaagcaaa ctgcccggcg aaagacatga agatggcgat
      721 catccctgcc atcacacccg gacgttgtaa taagcggaaa gtattttgtt tctgatgcga
      781 gggttcgcct ggcagtgaag gcaatgattt gataatccag aaaatacaca gcacgcccat
      841 caccgccgcc gcattaaaga cattgcgcca accgataagc tcgcctaaaa aactgcccaa
      901 cggcgcggca atcaccagcg caatagaaac cgcgccgaag atcaccgaca gcgccttcgg
      961 caccgtacgc ggcggtacca gacgcatggt cagcgacgcc gacatcgccc agaacccgcc
     1021 cagcgccagc cccagacagg cacgaccgat taaaagcaaa ctgaatgagt tagcaaagga
     1081 aaccagcaag caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt agcggcggtc
     1141 agtggcctga attgtctggg taataaacaa actggcaaac attgccacaa aggcggtcac
     1201 ggtcaccgat tgcccggcaa ccccttccga aatgcctaaa tcctgggcca ttggcgtcaa
     1261 caaactgacg ggcaaaaact caacgataat cagacaggcg acacaaaacg ccaccgagaa
     1321 aacggctgac cagttcggtc gggtgatggc atccgcgccg cggttttcgg caataaattc
     1381 actcatggtg ttacccgtgg catgtttcag gggaaaagcc gaccacttta acattgaaat
     1441 gtgtgacgaa tttaacgttt ttgcaacttt tacggtgttg cttgagctgg agttaagatc
     1501 gaaacggaac agggcaaaga ttaccggatg cggcgtgaac gtcttatcca cccgacaggt
     1561 caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt gaacgccctc
     1621 ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct gcatttcagg
     1681 ttcggtttca cgcagaaaac ccagcgccag cctgacctga gcatcggtaa tcggcgcgga
     1741 aaggcgggct ttttgtaaaa tccggtgcca ttgcagcagg ttctgttcgc tatcatcgac
     1801 aatgacaatt tgccagataa gcaacacctg cgcggcgttt tgcaggtgcg actcatccgg
     1861 gcgatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat cgaccatcga
     1921 ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg cttttctggc
     1981 cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg cgagcattat
     2041 ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc ctcgccagtt
     2101 actaatccac cagaataatc ttcaatgcga acagagctgc gaccaccacc acacacaggt
     2161 tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata aagccgagtg
     2221 caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata aacgcaggca
     2281 ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc agcacgccaa
     2341 caaagattaa cgctccagcg gttgcgtaag gaggaaccat cgccaccagc ggtgagaaga
     2401 acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg cgaccaccga
     2461 ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg ataaatgcgc
     2521 ctgccaccga actgacgcta tcaacataca gcgccttatt catattgggg aatttaccgt
     2581 tgctatctat taagcccgct ttatcagtta caccaattaa tgttcctgat gaatcaaata
     2641 ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac gcgccgctca
     2701 aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg ccgctaaaat
     2761 gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc agcaccgcgg
     2821 catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata cctaacaaca
     2881 cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg acaataacac
     2941 cagtgttttt taatcccatt aaggcaataa ataatccgat tccactggtg ataccaatac
     3001 gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc gtgagcaaaa
     3061 atagcccaat tgcgccccag aatatcgcgc ccatcccggt ctgccaggag atccccatcg
     3121 cccccacgac cacgaacgca aagaaggcgt tcagccccat tgccggagcc agcgccacgg
     3181 gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa caggtggtaa
     3241 caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc acaaaaacaa
     3301 tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg acggtggtcc
     3361 catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc acgtaatcgg
     3421 tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc ttttgtaaaa
     3481 caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt aatagatatt
     3541 ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat aaaatcaaaa
     3601 tgagaaaaat atgaataatt ccattaacca taaatttcat cacattagcc gggctgaata
     3661 ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta ttgataatgt
     3721 ctctattctc gacctgatca atgccggaga aatttccggc ccaattgtga ttaaaggacg
     3781 ttacattgcc ggtgttggcg cagaatacgc tgatactccg gctttgcagc ggattgatgc
     3841 tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg aatccagcat
     3901 gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga ccgttatttg
     3961 cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt ttgcccgctg
     4021 tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc ccgctctgga
     4081 aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct ggcgggacca
     4141 tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta acgggcagaa
     4201 tgcgctgctc gataaactcg atgcatttcg ctacctgacg ctggacggtc actgtccggg
     4261 tttgggtggc aaagaactta acgcctatat tgctgcgggt attgaaaact gccacgaaag
     4321 ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga tgatccgcga
     4381 agggtccgct gccagcaatc tcaacgcact ggcaccgttg atcaacgaat ttaacagccc
     4441 gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg aagggcatat
     4501 cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg tggcatatcg
     4561 cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct tactggcacc
     4621 cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg tgcagcaggt
     4681 actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt cggcgagact
     4741 ggcgctgtct gccccgccat atggcaatac cattgcccgc cagccagttt ccgccagcga
     4801 ctttgccctg caattcacgc ccggaaaacg ctatcgggtc attgacgtca tccataacga
     4861 attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc gcgatgatgt
     4921 gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct gtggtttgct
     4981 cggcggcttt ggcctgaatg aaggcgcgct ggctgcgaca gtcagccatg acagccataa
     5041 tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc aggtgattca
     5101 ggatggcggc gggctgtgcg tggtacgtaa cggccaagta caaagtcatc tgccgttacc
     5161 cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa ttgacgcctt
     5221 gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc agatggcgtt
     5281 tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat ttgatggcga
     5341 gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaaagcccg gcgtcatgcc
     5401 gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt ttctcgcggc
     5461 ggattttgcg ttcttccatt accgccacta tcgccatcag gcagatacaa ccaatcgcgg
     5521 cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccgaatacc ggcgtcccat
     5581 cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa gcaaaggtgc
     5641 ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca gcagccacac
     5701 caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa gccagataga
     5761 tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc gccagcgcga
     5821 tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc cacagcagcg
     5881 taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc gctttagaga
     5941 gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata cgtaccacat
     6001 agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc acatactcaa
     6061 caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct tcgctgatct
     6121 cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag tcgctgccgt
     6181 aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata aacatgccga
     6241 tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacacctgct gcgcctgcac
     6301 cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta cgacgcggcg
     6361 tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa aagccgctta
     6421 aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata ctggcactga
     6481 agcccagcat acaaatggca gagaggataa gcatgaacgg caggaattgt ttggtgtttt
     6541 tgccgtcggc gtagtatgaa accaacgttt tacccacgcc ataagtgatg gagaaaccca
     6601 ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata tcgttctgcg
     6661 cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag accaccaggt
     6721 aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga aggtccaggg
     6781 tcggcttgcg aacttggttt aggaaagcca gcatgggtta ctcctgaaat gaatacctgc
     6841 gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt ccaagaaaaa
     6901 cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc atcacgcttc
     6961 acgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc cggcggcgat
     7021 agagataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg ccagcggcca
     7081 accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg ccgcccctgc
     7141 cgcctctttg tgggaacact ctgccgccgc cataccaatt aacatctgcg ggccaaagac
     7201 aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa atggcatcag
     7261 ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca tcggcccacg
     7321 gttgccgtta aacaatttgt ccgagcccca accggctacc agcgcaccga taaatccgcc
     7381 cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca gcgtctcgga
     7441 catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca gcacatagca
     7501 aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga tctctttacg
     7561 cgtcaaccct gccccttctt gttgttgagc aatttccagc gcgtcgtgcc gccattcacc
     7621 gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc cgccagcaga gaaaaatccc
     7681 cacgactatc gccatacaac cagcaatcat catcccggca cgccagccgt aatgcagcgc
     7741 gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg tgttccataa
     7801 tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc gttaacagac gcgcacacac
     7861 cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa acgcccatag
     7921 cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg gcgataagcc ctatccccat
     7981 aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg acacgccgta
     8041 agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca cgccgttagc
     8101 aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg aagtaaaaca gcgcgtaacc
     8161 cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat caatttcatg
     8221 tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca tcatcgaacc
     8281 ttagacataa cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac aggaaatgtg
     8341 taatgtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa aaccttgttg
     8401 cccggaaccc ggcggcaaac cgctgccatc gtcttcaatc accagcatca accgttcatc
     8461 ctgctgccag ccttgcagcg taaccgcgct ggcatcggca tgtttcacaa tgttgttcag
     8521 cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaatg ctgattcatc
     8581 gatacgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt cccgcatcag
     8641 tgagcggatg gcctgctcca gggtgagatc atccagctgg cgcggacgta accgtcctaa
     8701 caaacggcgc accgcgtcgt aaacgcccag cgacagttgt tcgatgagct gcccgctctg
     8761 cttcacactg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag tacgaatagc
     8821 agtgatggtc tgaccgatat cgtcgtgcag ctcacgcgcc acgtcgcggc gcacgctctc
     8881 ttcggtttct aacaaacgtt cagccagatg ctgattgcgc gccagttcct tttgtagcga
     8941 ctggttaagt tcacgcaacc gctggatgcc tgcgcccagt aacaaccctg tcagactttg
     9001 caccagcaac gagagtaata aatccaccag atgatcgcgc caggtttgac tggcgatcag
     9061 cgcgatggcg ttcatcaacg tcgcaatcag cgctccttgc cagccatagt gccaggccag
     9121 cgcgataatc ggcagcgcca gacagaatgg cgtaaagcgc gacagttcgt ccggcaatcc
     9181 caactggagc cagagactga taacaaacag cagcaagtac cagaccagat gccgcccgcg
     9241 ccagttgatg ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt tattggcgag
     9301 atagtgccag aacaccagac atatcggggc cagcgtcaga ccgccagtta aagtcagcaa
     9361 cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca acaacgccgc
     9421 cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgctgat ggcgatagcg
     9481 cgagatcagc gctacgggca gtaacgtcag caaactaccg atcatcaata acggaaaatg
     9541 ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac ccagcaatac
     9601 gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa acggaaataa
     9661 cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccata ggcaaaacca
     9721 tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga acaacgtctt
     9781 catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac gcccagtttt
     9841 tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag ttcggcggca
     9901 atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc acgtttggtc
     9961 agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag ataacagccg
    10021 cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg tttggaaaga
    10081 aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc gtgaacggag
    10141 agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc ggagatatcg
    10201 ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc cgccagcgcc
    10261 tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc cagcagctgc
    10321 gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat catggtcttg
    10381 tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt gttcaagcct
    10441 tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg gactgattac
    10501 gctgcacttt cacgacatct tccagcttag cgatttggct tatcatctgc tccagacgct
    10561 ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc ggcagacaaa
    10621 gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg gtcattacgc
    10681 ccggatggtt gcgaacggtg agctccagaa ttacgttgtt atgagttgtg ttttgcatgg
    10741 cttattcccc caccatttca gtattcgccg cacctggcgg caccatcggg taaacttttt
    10801 cttcggcatc aatgcgcaca tggatcagcg ccgggccagg gcgattgatg atttcctgca
    10861 atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg aatccggcgg
    10921 caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct tgctcgtaga
    10981 acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga atgattttga
    11041 catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc aggctgccgt
    11101 cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca atcgccgcag
    11161 gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt gggcgattga
    11221 gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg ataatcgcat
    11281 tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat ggatcgcacg
    11341 ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg tgccactctg
    11401 cacgcggttg cgcttccacc cgcgggatca attgcgccag cacgtcatca acatccgcct
    11461 gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata tcgacatgaa
    11521 taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg
    11581 caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg cgcacgccgt
    11641 gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc agcgccatta
    11701 aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc gcaggcgcat
    11761 tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc attgtagctg
    11821 cgtcacgaat gctttcttcg ctaaaggcgg gtgcaacggc tttttctgcc accgcgggct
    11881 gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc cacaccgggc
    11941 tagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgtggg agctcttcga
    12001 tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag gtgtccactt
    12061 cttggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg caaatcagcg
    12121 ggatggagtc cagccgcgca tcagcaatgg cggtcaccag gttagtcgca cccggcccgc
    12181 tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga gcgataaacc
    12241 ccgcgccctg ttcatggcga gccagaatat ggcggatttg cgtgctttgg cttaaggcgt
    12301 cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac aatcttaatg ccctgctgtt
    12361 ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg
    12421 aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg cttaaacgaa
    12481 aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc agtccctacg
    12541 gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat ggcgcagttg
    12601 gtagtagttt tgcgttgaac atggaagtag tcattgggga ccttgttggt tttgtgttta
    12661 acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt cagcaaggga
    12721 aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac cccgccggag
    12781 cgaggtttcg tcagccgcct gcggctggta accgcaaagc acactgtatt atgtcaacac
    12841 tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg acgtatttag
    12901 tgcatagttg agtatcgttc acagtttgcg ttttgtccaa atattactgt ttatttatac
    12961 agtaagcttc tataatatca ctgtacgcaa tgtgttatgc gggggccgca tcgttacccg
    13021 gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc gcgtgagcac
    13081 actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca caacagctgg
    13141 caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg agacgcgtat
    13201 gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac tgcaactgct
    13261 tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa ggggagcggg
    13321 taaccgctcc ccttttatat ttagcgtgcg ggttggagtc ggatgcgatg ctgacgcatc
    13381 ttatccgccc taccatccct cccggcaaca tttattgccg cttttgttta catattctgc
    13441 cgctaaacaa ttccccattc ctggcgtata tctggctaac attcatcaat gtgatagatt
    13501 cctctcccgc atttatggga atgcgtagtg acttattcta attattttta taaaagcatc
    13561 cgtgataatg aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat tactcgtggc
    13621 cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg cgcgcgatct
    13681 caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga cttacggtgt
    13741 ctcacagctg ttttatggcc cgatatccga ccgtgtcggt cgccgaccag tgatcctcgt
    13801 cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca gtttgacggt
    13861 attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg taatggcgcg
    13921 tactttgccg cgtgatttat atgaacggac acagttgcgc catgctaaca gcctgttaaa
    13981 catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc tggatacgat
    14041 gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg tgaccttcag
    14101 tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc gcctgcttac
    14161 cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga tgctgattgg
    14221 cggtctggcc gggattgctg cctttgaagc ctgctccggc gtgctgatgg gcgcggtgtt
    14281 agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg cggcattttt
    14341 tggggcatgg tttgccgggc gtcctaataa acgcttctca acgttgatgt ggcagtcggt
    14401 tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg gcgtgatgaa
    14461 tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga tgctgtttcc
    14521 gctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg ctggtgcgct
    14581 ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg cgatgttgcc
    14641 gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt tgatcgtgct
    14701 gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc cgtttaagcg
    14761 cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt atcagatcac
    14821 gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc gcacaaagca
    14881 cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg cacgcctggc
    14941 agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg gcgtcaaaaa
    15001 gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg gcaattaaaa
    15061 atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg tcatcaaatt
    15121 caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg tgataaaaat
    15181 cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc tcttcagcat
    15241 caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt gatgtatatg
    15301 cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa atttgtcacg
    15361 gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc gattacgggt
    15421 gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc agaataacat
    15481 gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt agcccagcat
    15541 ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg caatgcgtct
    15601 ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccgcc acaaccatca
    15661 atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc agacgcatcg
    15721 ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg gaaaacagac
    15781 acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc gcaagttgat
    15841 caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc agaaaagtac
    15901 gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa attttcattg
    15961 ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa gaatttcgtc
    16021 cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg gtatcatagc
    16081 gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa tgatggggat
    16141 tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa gcatcgtggc
    16201 ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg aaaaaattac
    16261 cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa gcggtatcgc
    16321 actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg aaaatcgctg
    16381 gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt tgctgatatt
    16441 cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa taatgcccgc
    16501 cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc caccttcact
    16561 ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc atcttgtggg
    16621 cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg acgcagactc
    16681 cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa ggcgtcatgc
    16741 attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat ttcatttggg
    16801 tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag tttaaagtca
    16861 tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc aatctcgtag
    16921 cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt ttcccccggc
    16981 agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa atcaatatga
    17041 ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat cagcggaata
    17101 cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc gcccatcatt
    17161 tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg cgttaaggca
    17221 ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata gagcggatgg
    17281 tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa gcggtgatgt
    17341 tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta aaaatcagcg
    17401 tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc atacgaaacc
    17461 accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac cgctcgattg
    17521 ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa atcttcgacg
    17581 ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct aagatagttc
    17641 gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt gccgaaatag
    17701 tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc atccttaaag
    17761 tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca cgggccggac
    17821 tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac cggtgaacaa
    17881 gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt ttgcgtattc
    17941 agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt caattggggc
    18001 gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt gacgatgcca
    18061 ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac tgatcatcgc
    18121 taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc caatcatcgc
    18181 gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt ccaccgcaaa
    18241 cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca ccagcatcac
    18301 gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt cgaacttcac
    18361 cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt ttgccgccag
    18421 cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg gcacgttgta
    18481 aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttcg cgatccacgg
    18541 cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga cggtgaccag
    18601 ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac tgaataatgt
    18661 actggcgcta ttcagaaagc cgttgaaggt actgatcacc gcaccaaata acaccgcgcc
    18721 gaagaaaccc tccagtggca ctggcagaac gttattgacc agcgtcgggt aggccatgtc
    18781 ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca ccagtaccag
    18841 cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt ccgccaggct
    18901 ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa aggtgttcac
    18961 cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc caccgactga
    19021 gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa agctgccctt
    19081 gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca gcccaatacc
    19141 gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat acaaaattcc
    19201 cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa tctgtagcga
    19261 ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa caatcggcag
    19321 aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg tcgttttatc
    19381 ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat agcgcggtaa
    19441 aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc ccatcaccga
    19501 catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg aaaggttggt
    19561 taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa gaaaatagcc
    19621 ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg ccaccagcag
    19681 cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct tatttatgtt
    19741 tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg cagtcagatt
    19801 cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc aggttgtgag
    19861 cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag atgtaaaaaa
    19921 cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca gcggaatttc
    19981 tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct ggccgcggga
    20041 caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac aaggatgtgg
    20101 gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta ttatattttt
    20161 aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac agtactggat
    20221 ggaatttacc cccaccagta tgatggatat ttccgttggt cagcaaagcg ttatttataa
    20281 tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt caccagaagc
    20341 aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt ggatttgtct
    20401 tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat taattgccac
    20461 tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca cgatcccctg
    20521 tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc cgaaagaata
    20581 ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag gaaactcggt
    20641 tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca aagcctttaa
    20701 acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc acccaacgga
    20761 tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct ccagcaactc
    20821 atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg taatcgcctg
    20881 ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt ccaccgccac
    20941 ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca gtggttcagg
    21001 ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg cgccatccgg
    21061 ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg gcgcagacga
    21121 cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta ggcgtttggt
    21181 gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg catgccgtac
    21241 gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag tgggattttt
    21301 tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac aaaatcaacg
    21361 tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg cgctttttct
    21421 ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc gttatcataa
    21481 aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa cacgatgcct
    21541 ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat tatactgcct
    21601 ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg agctgttcac
    21661 gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta cggaagacgc
    21721 cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg tcatgcagtg
    21781 caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc ccgcccaggg
    21841 cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg gtggtttggc
    21901 ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct tcacgtcccg
    21961 gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac agcagggtaa
    22021 agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg aacatcggga
    22081 tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc accacaccaa
    22141 agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca aacagcaacg
    22201 gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc atggcggtta
    22261 aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg gcagtgaagt
    22321 acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc agggcaaaac
    22381 cgccttccgg gaacaacgat ttcagtggct cggcgctcag actgaactct tgcagatgct
    22441 gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata aacggtccgt
    22501 agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg taaacccaca
    22561 ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc atttgtactt
    22621 ttggccagcc gagcaatgtc agccaggcac aggggatcat caccaggaag gcgataatca
    22681 ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta tcgaacagac
    22741 ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg ctggtatcga
    22801 gggttttaat cccggccatc attgtcagac cgctacctgc caccgcgtcc tgagtgaaat
    22861 cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag ttccaggtca
    22921 ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca aggccaatgg
    22981 gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg ccctcttcaa
    23041 taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc ccgacaaaca
    23101 tcgggttttg cagcaagatg gcaagaccca ccacaatccc ggcgaaggga aacagcagca
    23161 ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact gagcatttcc acatcctctt
    23221 ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg cagacctgga gcgaggatag
    23281 gaagatgcga tcggcagaac atgacgaccc aatgggattc gtgatcgctt tcatgctttt
    23341 cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga catctgtgag cacaggtaca
    23401 cagcatcgaa cacatcttta aaaaaaagat ttttttcaat cgattaagca gaacttgtgg
    23461 gcgcattacc cgtgcttgca ggcaaaaaag agagctagag atgatctaca aaagcattgc
    23521 ggagcggtta agaattcgac ttaattccgc ggatttcacg ctaaacagcc ttcttcccgg
    23581 tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg actatccgta aagccattga
    23641 cctgctggta gcgtgggggc tggtggtccg ccgccacggc agcggcactt acctggtgcg
    23701 caaagatgtg ctgcatcaaa ccgccagcct gaccggactg gtggaggtgt taaaacggca
    23761 gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc ctccggccat
    23821 tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc gcgttcgttt
    23881 tgtggaaggg aaaccgctaa tgctggaaga cagctatatg ccggtaaaac tgttccgtaa
    23941 cctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag agtgcgggat
    24001 tttgattggc ggtaattatg aaagcctgat gccggtgctc gccgatagac tgctggcgcg
    24061 gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt catatagcga
    24121 gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat actaggtgga
    24181 gtaccattta cggcgactcc acccggaaaa aagttaaccg atactccaga agagcaccgc
    24241 cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga
    24301 gagcgccgcc gcaccactgg ttggatgaag attattagca aacgccagcg ccggaggatc
    24361 ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg
    24421 cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca
    24481 gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt tcagaccaac
    24541 gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac ttggcggcat
    24601 aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca taatcagcgg
    24661 accgcccgcc agccccagtt tcaacgcggc cgggaatcct ggcacaaaga cgggaataga
    24721 acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa cctgttgcag
    24781 tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac
    24841 caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca gttcgacccc
    24901 ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat
    24961 acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgcc
    25021 tttcgtagac agcgaggtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc
    25081 cgctggctgc cccaccagat gcagcaaatc gcccagttgg ataatggtat ctggcgaagg
    25141 tacttttagg gtttcttctc gtttcagacg cgagcagata attttgtcgc cgttgagaat
    25201 cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa tattgatagt
    25261 cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt ctgtctcgac
    25321 attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc aaatgccgaa
    25381 tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg gtgtacccag
    25441 gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta ttggttaccg caccggagaa
    25501 aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag
    25561 accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa
    25621 tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca ggatcaggcc
    25681 aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct gagaaacaaa
    25741 atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta tgccgcgaaa
    25801 tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac tgaccgttaa
    25861 tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta cggattatgg
    25921 ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag aacgtgccga
    25981 aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca
    26041 ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat
    26101 agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa tatgcagtaa
    26161 accgtttacg aaggttgcgc cagagacttc catattttca gccagcgtaa aactcaggct
    26221 aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg
    26281 cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc tctaaatctt cctgacggaa
    26341 acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg
    26401 cgggaagctc tggctttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat
    26461 ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa
    26521 gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga
    26581 tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt tcgatacggc gcggtttttt
    26641 cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcccc
    26701 acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc
    26761 ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag
    26821 attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat
    26881 gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg
    26941 gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag
    27001 cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac
    27061 cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcgactac
    27121 gatgtaaaat gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag
    27181 tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc gatagccgcc
    27241 acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca
    27301 acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat
    27361 atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata ccggggggaa
    27421 agaaggaacg tatcccggca cccgcgccag cgccaccatg attggcgacg atgaaaccaa
    27481 ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtaa tggacacgct
    27541 tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcgcgcg ttgaaaaaag
    27601 cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc ctgataactg
    27661 atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc cagccattga
    27721 ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa
    27781 tactcaacct cgccgctttg cgcatgggca caaacaattc gattatccag cacaaagccc
    27841 aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt gttatgggtt
    27901 aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca gttgcacaat gccgttatca
    27961 tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg
    28021 acgttgacta accccggata agcccgatga tgggtaaagg ttaaacgtcg ctgcacgacg
    28081 ccacgcggtg gcgcgggtag ggttgtttcc gttccactta acggcgcatc acctgccgat
    28141 ttccagctag cttcatcttg cggtaactca acgataaata gctccggcac tttctcgccc
    28201 tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca tccttcttca
    28261 taagcacgat tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt actcaccagc
    28321 acacaccagt ggctaccgct gtattcacgc ggatgttgct tttgtacgtt gaccgggcca
    28381 aacggtgcag caaccccgac gtttcgcaaa tccagcgccg gatcgagttg atgcattaca
    28441 tggtcgttat aggtaaagct ccccctttca ccgttcgggc taaagacatg cacatggctg
    28501 ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc gagattgctc
    28561 accttgccgc cttcagcaat gactccgcgt cgatgatgga agtcgtaatg ccatgtttca
    28621 tcaggatttt cagggccgtg aataaagaca tatttctctg acttcggatg aacggtcacc
    28681 acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc tgtatggata
    28741 ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac gtcaaatacc
    28801 agccactggc tgtcgggcgt ccaggtattg gtatttgtat tggtattggt attggtgagc
    28861 aggtgattac ggggagcaaa ggttatctgt ttcatggcgc ggtcctgata caaaacggtt
    28921 cccgccatca tacttcacaa ggacttcact ttcagacaat agtcttttgc catcgtgatg
    28981 aatgtgagtg aaaagatgga acatttcgac gtggcgatta ttggcctcgg cccggcaggg
    29041 tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga tcgcgctgga taaaaagcac
    29101 cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc tgctggcacc ggacgcgcag
    29161 cgatctttta ttcgcgatgg actgacgctt cctgtcgatg tgatcgccaa tccacagatt
    29221 ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc gtaactacca gcgaagctat
    29281 atcaatatta atcgccatgc tttcgacttg tggatgaaat cgctgatccc cgccagcgtt
    29341 gaggtttatc acgatagcct gtgccgaaaa atctggcgtg aggatgataa atggcatgtc
    29401 atttttcgtg cagacggctg ggagcaacat attaccgccc gctatctggt cggtgccgat
    29461 ggcgcaaact cgatggtgcg gcgacatctc tacccggatc atcaaatccg taaatatgtc
    29521 gctatccagc agtggttcgc agagaaacat ccggtgccgt tctactcctg catctttgat
    29581 aatgcgataa ctgactgtta ctcatggagt atcagcaaag atggttattt tatctttggc
    29641 ggtgcctatc caatgaaaga cggtcagacg cgtttcacga cgctgaaaga gaaaatgagc
    29701 gcctttcagt tccagtttgg caaagcggtg aaaagtgaga aatgcacggt gttgtttccc
    29761 tcacgctggc aggattttgt ctgtggtaag gacaacgcct ttctgattgg tgaagcggcg
    29821 ggatttatca gcgccagctc gctggagggg attagctatg cgctggatag cgcagagatt
    29881 ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg ccgcttactg gcgcgccaca
    29941 cacaaactgc gtttaaaact cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg
    30001 gctttaagaa agtggattat gcgcagtggt gtggcgcata ttccacagtt gaaagattat
    30061 ccaacgcgct tcacatcacc caccagcagg atgtaagaga gcgcgccaat caacgcgacg
    30121 gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct gcgccaggta ccccaccacc
    30181 agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa acacgccgcc ggttaaacca
    30241 atcaggcgca tcggtgccag agaagagacc agcgaccagg taatcgaagc aaaaccgttg
    30301 ccgaagaatg ccagcgccat caggcacata atcatcatcg gatcgttggt gtagttagcg
    30361 cccataatgc aggtggagat cagcaagccg cagataatcg gcgttttacg cgcaaagccc
    30421 agtgaaaagc ccttacgtac cagcagatcc gcgacccagc cagagagcag gacgccgaca
    30481 aacgccgcga ggaatggcac cgtggtcata aagcctgctt tcagcgccgt gattcctttt
    30541 tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc acagtgcaga agccaccgca
    30601 aattgcccaa gataaacgcc gatcagttta cgatggaaca ccagtttcca gtctttggct
    30661 gttaacggct gacgcgcctc tttcttcacc ggcgcatcac catccaccag accgccgcca
    30721 tcacgaatgt aatccagttc agctttgctg atacctttgg tcaggcgcgg cggctgataa
    30781 accttaaacc aaatcagcga ccagataatg ccgataccac cagtgacaat gaacacccag
    30841 tgccagctca acatctcctg aatccagatc agcagcggag tcaaaaacgc cagaccgaca
    30901 aactgaccag acgtataaaa accaacggcg gaagcgcgtt catgttccgg gaaccagctg
    30961 gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc
    31021 agaccaatta atgacattaa tcccgtggca aagccttgga ataaagtcgc cactgaccaa
    31081 ccaaatatcg caataaaata agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg
    31141 gggatctggc atagcgtata aagccaggcg aaggccgaaa atacatagcc catttccgct
    31201 ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca cggccaggtt agcgcggtca
    31261 acataacaaa tgaccaccgt aataaagatc atcaccagcg tcagataacg ccgacgcccc
    31321 ggctttgctg cattaacggg aatatccata gcgagctttc tccagatttt gggcatagcg
    31381 aagccgctca ccatgccctg taatttacag agggttatat ttttgtattg ctgttttagt
    31441 gcccgatgag gggcttacgt ggcaggaatt accactctgc tacgctgtta tcttcatgac
    31501 gccagagcgg attacgccag tccggggcat ttttactgaa ctcaataact ttagcttcgt
    31561 cgatttccac acctaagccc ggtttcgtta acggtttaaa gaagccgcca accatgctga
    31621 agtcttcttt gtttttcaca aagtcgagta actccgcgcc tttgttgtaa tgaatgccca
    31681 tactttgtta ctgaagtacc gcgttatagg aaacgaagtc gatatgcagg caagccgcca
    31741 gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac atcataggct tctgccattc
    31801 cagcaatttt gtagcattcg gtaataccgc ctgcatggga gagatccggt tgcagaatcg
    31861 aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg
    31921 ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta gtattcggct tgttccgcca
    31981 gcaccggctc ttcaataaac agcgggcgat acggctccag ttctttaatc agcactttcg
    32041 ccattggcgc gctgacgcga ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag
    32101 cttcacgaat ttgtgccacg gtgttgactg ccgcatctac cgcgcgggag ttatcaatta
    32161 gccccagttc ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg cgcagggttt
    32221 taatgccgtc gataacatcc gccggacgat cgccgccgac ccaactgtag gctttaattt
    32281 tgtcgcgaac caggccgccc atcagttgcc agactggcgc attcagcact ttgcctttga
    32341 tatcccataa cgcctggtcg atcccggcga tggcgctcat caggattgga ccgccacgat
    32401 aaaatccggc gcgatacatc acttgccata agtcgttgat gcgcgaagga tcctgaccaa
    32461 tcaaatagtc acccagctcg tgaactgccg cttccaccgt acgggcgcgg ccttcaatca
    32521 cgggctcgcc ccaaccgacc acgccttcat cggtttcaat tttcaggaac atccagcggg
    32581 gaggtaaacg atacgtggta attttggtga ttttcattgc actgcctctc gatacgcctt
    32641 aacaaatgct gctgcctgct gcgcggtgcg ttctacggac tgcccggcgc gataaagatc
    32701 gctgcctaag cccgccccaa cacaacctgc gtctatccac tgcgccaggt tttctggcgt
    32761 cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat accgctttta acgctttgat
    32821 gtattgcgga ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag
    32881 cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa acggtcatgc cgtagcccac
    32941 cgcgcggcgg atcacttcac tatggatatt gggcgtaacg atgagctgac agcccatcct
    33001 ggcgagcgca tcgacctgtt caggtttcag taccgtacct gcgccaatca acgccttgtc
    33061 gccatacgca tcaacgatgg cgggaatgct ctgctcccat tgtggggaat tcagcgggat
    33121 ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg
    33181 cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt tgccactgca tgagcgatgc
    33241 tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc cgccacgtcg caacccatcg
    33301 cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc ggcgacaagg gtgatggcgt
    33361 gttgatgggt cacatagtcg cgcatactgg caacctctgc gccaatcaac aaaccagaga
    33421 gaaattcgct gacctgttcg cggggaagtg ttcccagcac atgcgaggcg cgaacttcaa
    33481 aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc aaggccagcc gcgaaggcat
    33541 cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt aacagtaaat
    33601 gatgtaattc accggtcatc acggtgcgaa aatcgttgat ttgctggcta tcggcctgca
    33661 cccatttgca atgcgtaccg ggcataacat aaagagagga aggagccaga gctcgcgcgc
    33721 cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt atcgtcatga gagacgcata
    33781 atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa
    33841 aacgggcagg aacagataaa tacggagcaa ctttccagcc gacattgctg ccgaccattc
    33901 ctgccatcac cactggcgta ttttcttcac gccagtcggt cgtgacttct gctaacactg
    33961 cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc
    34021 agtggtcgcc ctgataaagc caggcgcgca gattggtcga tccccagtca attgcgatgt
    34081 agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcaatca tggtaagcgc
    34141 cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc
    34201 ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa aaaccgcccg
    34261 ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc gggttatgca ccgactgcag
    34321 caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct gattgttggc
    34381 aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg cgcgctctgc
    34441 tgcccaacgg gcaatcgccg gttccaccag atttcgcact tcgctcatgg cactgataag
    34501 ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca
    34561 ctggttacgc ggtgccacaa acgccccgcg ataacgtttc atttcaatca gccgcttcgc
    34621 catcagcgaa cggaacacct cacggatgat gttgcgcgag gttgcaaact cttcacagag
    34681 ctccgcctca gccgcaagtg gcgagccagg tacgtatttg ccgtgaacga tctgtttacc
    34741 cagcgtaatg acaatgcgat cggttttatt gagagtcatg gagagtcctt gtgctctgta
    34801 tgttcttctc tactttaccc cgatcgatgc ataacgcggc aactttgtag taccagcgtg
    34861 atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac ttatattgtt gcgctacaat
    34921 ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc gtcgggcaga aatctgaaaa
    34981 gggatatcag gcgctaaaca ggagggaaag aagagtatgc tttcaacggc ttagctactc
    35041 gtttaaagga ttaatcatga agttgaattt taagggattt tttaaggctg ccggtttatt
    35101 cccactggcg ctgatgcttt caggctgtat ctcgtatgct ctggtttccc ataccgcaaa
    35161 gggtagttca ggaaagtatc aatcgcagtc agacaccatc actgggctat cgcaggcaaa
    35221 agatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt accttatcac
    35281 tgatggtgcc gatgacatcg ttaagatgct caatgatcca gcacttaacc ggcacaatat
    35341 tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac
    35401 aatatcgctt tactactact ggaataacga agaagaaaag gcactggcaa cgcattatgg
    35461 ttttgcctgt ggtgttcaac actgtaccag gtcactggaa aacctaaaag gcacaatcca
    35521 tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg ttctaccatc catttaaagt
    35581 gcgattttat gaatactatt cacccagagg cattccggat ggtgtttccg cagcattact
    35641 gccagtgact gttacactgg acatcattac tgcaccgctg caatttctgg ttgtatatgc
    35701 agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt
    35761 aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggttg gattttgtga
    35821 caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca ccgacgcctg
    35881 catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt tcttccggtt
    35941 taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg cgtttatcga
    36001 ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc acttcctgcg
    36061 gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc aatcatcatc actttcagga
    36121 actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaatt gtggcaacga
    36181 aggattcatg caccgtgtag tagctgatat cgcggttagc ggtgtacagc gtggtgcggt
    36241 ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt
    36301 catagctgag agcggtttgc gccacggtgc taccatcagc ggccttctgt accagcgcgc
    36361 cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc aggtagttgt
    36421 gcacacctgc atacggacga cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg
    36481 cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat
    36541 ccatatcgat agcaatgagt ttgatagcca tgagttccca aggtagatta gttcctgacc
    36601 catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag gggatgcaac
    36661 gcgggttgca tgctccccat ttgcttagcg gaagagtttc cctttcagca gttccatgcc
    36721 tgcggaaagc agatcgttat tggcttgtgc tgacacttca ccttgcggtg agagcgcatc
    36781 aataatcttc ggcaattgct ctgccagtaa actggaagcc gtactggtat ccacgccaag
    36841 tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gactccagtt gctcgccgct
    36901 aactgattga tttctctgtt gattactcaa ccaggttgag agaatggccc ctaagccgcc
    36961 actttgcagt ttttccagca gcacctgaat gccaccctgc tcctcaaccc aacttaaaat
    37021 cgcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat caaaaagccc
    37081 catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatc tgcgggatga
    37141 taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt cttatagctt
    37201 cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa atcatgcaaa atcaatatat
    37261 tgcaggagtc tcgtaggact aataagcgta gcgcatcagg cacgctcgca tggttaacgc
    37321 cattaaatat caatattcgc cgctttcagg gcgttctctt caataaacgc acgacgcggt
    37381 tctacggcgt cgcccatcag cgtggtgaac aactggtcag cggcaatcgc atccttaacg
    37441 gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag ctgttccggg
    37501 ttcatctcgc ccagaccttt ataacgctgg atggagaggc cacgacggga ctctttcacc
    37561 agccagtcca gcgcctgctc gaagctggct accggctgac gacgctcgcc acgttcgata
    37621 aacgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat acgacgatat
    37681 tcgccaccgg tgataaactc gtgatccagc ggatagtcag tatccacacc gtgggtacgc
    37741 acgcgaacaa tcggctcgaa caggttttgt tcggcattgg tgtggacatc aaacttccac
    37801 tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac ccagcgggta
    37861 acggtctgct catcagaaag gtcagcttcc gtcaacgtcg gctgatagat aagctctttc
    37921 agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt cgcgttgtac
    37981 tcagatacca gtttctctaa cgcttcgcca gccaatgccg gtgcactggc gttggtgtgc
    38041 agcgttgcgc cgtccagcgc gatagagatc tggtactgat ccatcgcttc gtcgtcttta
    38101 atgtactgtt cctgcttgcc tttcttcact ttgtacagcg gcggctgagc gatgtagacg
    38161 tgaccgcgtt caacgatttc cggcatctga cgatagaaga aggtcaacag cagcgtacga
    38221 atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata acgcagtttg
    38281 tccgggttgt actcgtcacg accgatacca cagccaagcg cggtgataag cgtcgccact
    38341 tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttaaggat tttacccttc
    38401 agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc gcccgcggag
    38461 tccccttcca ccaggtacag ttcggaaagc gccggatcgc gttcctggca gtctgccagt
    38521 ttgcccggca ggccagctaa gtcgagcgca cctttacggc gggtcatttc acgcgcgcga
    38581 cgcgccgctt cacgggcacg ggcagcatcg ataattttgc caaccacgat tttcgcatca
    38641 gttgggtttt ccagcaggta ttccgccagc agttcgttca tctgctgttc aaccgccgat
    38701 ttcacctcag aagaaaccag tttgtctttg gtctgggagg agaatttcgg gtccggcact
    38761 tttacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc gctgactttg
    38821 gcttttttgc tgtagccttc tttgtccatg taggcattca gggtacgggt catcgccgca
    38881 cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt aaagcagtag
    38941 atgttttcct ggaagccatc gttccactgc agcgccactt caacgccaat accgtctttt
    39001 tcggtggaga agtagaagat atttgggtgg atcggcgttt tgttcttgtt cagatattca
    39061 acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc acgcttgtcg
    39121 cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg tttcgccaga
    39181 atttcatatt cgaactcggt cacattggtg aaggtttcga ggctgggcca gaaacgcacc
    39241 atggtgccgg ttttttcagt ctcgccggta accgccagcg gagcctgcgg tacaccgtgt
    39301 tcgtagatct gacggtgaat tttaccctcg cgctgaataa ccagctccag tttttgcgac
    39361 agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt ataggagtta
    39421 tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc cgatacgccc
    39481 tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga gacagagtta
    39541 tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc gtcgatagcg
    39601 ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt gtcgccgata
    39661 tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat actggaggag
    39721 tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat ccgttatttt
    39781 acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc
    39841 actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg caagcaaccc
    39901 gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac accgccgccc
    39961 gctttcacga gtgaggaact ctccttgcgc cagacgtaag gcgcacatca acagcttaag
    40021 ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc
    40081 tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat tacgttccag
    40141 cacctcagcg tactctgtct ctttctccca gccgcgctgg aaagagaaag tcagagaaaa
    40201 ctcagggaga aattgcttac aggtatcatc catatcggcc gcgataccgg cgctatactc
    40261 cgcgcgccag gtgctgattt gctccgccag cgggatcagt tctttatccc acgggcgtag
    40321 ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag
    40381 attgctccag gcggtgaaaa atccaggttc gttgtgaaag catccccagt cgaggaatgc
    40441 tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta tcaactgcat
    40501 cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc gtaccgtcaa tacggacttt
    40561 gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc tcgcgctctt cgccctgtaa
    40621 tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg acgcgaccaa tctgcaaact
    40681 gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc agcacgctgg ttttgccact
    40741 gccgttggca cctaccagaa agttaaagcc gggagataaa gcgagatccg cggtttcaat
    40801 attgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat tggcatgaca
    40861 acataggcag ccgactgtga tgcggcatct tcaatctgca cgctggaaac cgaatcggtc
    40921 agcatcatgc ggacgttttc gcatttcagc gcgttcagaa catccagcac atagctgacg
    40981 ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc ttccgcttct
    41041 tcctgttccg ggttgttggc agtaattttc agctggtttt cgctgacata caggcgcaca
    41101 ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt gagcagatcg
    41161 cagcctgctt ccagatgttt gtccgggttc ttcggcagaa cgcgacggta atccgggaag
    41221 cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa cgtgggcacg aatgttattg
    41281 ctgccaatct gcacacgcag cagattgtcg ccgccgtcga gcatacgcat cagttcaatc
    41341 acgcctttac gcggtacgat caccgaatgg cttggcaaag attgaccaat tggcattgaa
    41401 cagaccgcca gacggtggcc gtcggttgcc acggtgcgca gttcttcacc ttcggtttca
    41461 aacagcatac cgtttaagta atagcgaaca tcctgatgcg ccatcgaaaa ctgggttgct
    41521 tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct ctgccagtca
    41581 tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg aaaaacggct acgcccggag
    41641 cgcaccagca tccgttcacc ttccagctgc acggcaattt ccgcgccttc aggcagacca
    41701 cggcagatat caaagaattt gcgcgccgga acggtcgtcg ctcctggctc gtgtggctga
    41761 accagcgcaa cgcgtgccac catttccatc tcgagatcag taccggtcag cgacaacgta
    41821 ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg accacctaac
    41881 ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt aaatttcata
    41941 ggtttacgat gacaatgttc tgattaaatt tgaaaaatct tctttgatat cgtggctctc
    42001 ttcacgcaac tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt cacgcccacc
    42061 aaacgcatcg ccaatctctg gcagactgtg gttagtcagc tctttcgcta gcgccatcgc
    42121 catctggcgc ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt
    42181 gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca gtttttcctg
    42241 caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt
    42301 aaagttggcg ttagcaataa cgcggttcag cgccccttcc agctcacgta cgttagatcg
    42361 tagacgcttg gcgataaaga acgccacttc acccggcaaa cgaatgtcat tttcgtcggc
    42421 ctttttcatc aggatcgcca cacgggtttc cagctctggc ggttcgatcg ccacagtcag
    42481 tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg
    42541 atccgaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg tgtggaaaaa
    42601 ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca gcagtgcatc
    42661 tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg ctttaaccat
    42721 gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct tgcgcgccat
    42781 aatgccgtta cccaccgcat gcagcaggtg agttttaccc agacccgtgc cgccataaag
    42841 gaacaacggg ttataggcac cgccagggtt atccgccacc tggcaagccg ccgcgcgcgc
    42901 cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga
    42961 acgataggtc ggttctgccg gggccgggac gttatcccaa cctgagcgcg tagaaggcgc
    43021 agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc
    43081 cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct gtggggcatc
    43141 cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt cccgtaccca
    43201 atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca gttccgcctg
    43261 caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca atcgggcaag
    43321 acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag tcgataatga
    43381 ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgct tgagggtgac
    43441 gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg
    43501 cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt ccctatttat
    43561 ccacaggact ttccagaact cgctaagtgt aaacgatcct gccgcgaggc gggcacgatt
    43621 tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat cgatctaata
    43681 aagatccagg acgatccttg cgctttaccc atcagcccgt ataatcctcc acccggcgcg
    43741 ccatgctggt ttccactggt gtgaggtcgt acattttccc tgcgaaaagg tgcggaaaag
    43801 cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca attattacaa tccggcctct
    43861 ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc caataaagcc
    43921 attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca accgtctgta
    43981 ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag
    44041 gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa gtaataaagc
    44101 taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa ctcccagtca
    44161 attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta ccattctcgg
    44221 ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga aaaacgttcg
    44281 acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt gaaagcttcc gtctgcgcca
    44341 acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga
    44401 taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc gcctggctcg
    44461 cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct actcgggccg
    44521 cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg caggtttgga
    44581 gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc tttacaccct
    44641 ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta acgatggatt
    44701 cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct
    44761 gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca acgaccaccg
    44821 cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg
    44881 ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt gagcaagctc
    44941 tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg ttggaaactt
    45001 caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc ccggataacc
    45061 cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg ctggctgaag
    45121 gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac acgtttacca
    45181 aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac gtgcagaacg
    45241 ctggcgagaa accgctggaa atctcctcgt ttggtcagtt gaagcaatcc atcactctgc
    45301 caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt ggcgcggcgt
    45361 actccacgcc tgacgagaag tatgagaaat acaagttcga taccattgcc gataacgaaa
    45421 acctgaacat ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat ttcgcgacgg
    45481 cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg ggtaacggca
    45541 tcgtcgctat cggctataaa tctcagccgg tactggttca gcctggtcag actggcgcga
    45601 tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa aatggcagct gttgctccgc
    45661 acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagccg ctgttcaaac
    45721 tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc atcatcacct
    45781 ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc atggcgaaga
    45841 tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat gacaaacagc
    45901 gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg ctgggcggct
    45961 gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg ctgatgggtt
    46021 ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg gcacaggacc
    46081 cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag aagatgtcgc
    46141 cgaccactgt gaccgacccg atgcagcaga agatcatgac ctttatgccg gtcatcttca
    46201 ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta ctatatcgtc agcaacctgg
    46261 taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc
    46321 gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc
    46381 gccttttttc ttttcgtagg gcggataagc accgcgcatc cgccacacaa agcaacagga
    46441 acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga cgtggcggcg
    46501 ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt tgccgaaacc gtgctgggta
    46561 aactgcctaa gccgcgctac gccgattatc ttccgtttaa agacgccgac ggcagcgtgc
    46621 tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc gaagatgtgc
    46681 tggaactgca aggtcatggc ggtccggtga tcctcgacct gctgttaaaa cgcattctga
    46741 ccattcccgg cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg tttcttaacg
    46801 ataaacttga cttagcccag gccgaggcga ttgccgatct tatcgacgcc agttcggaac
    46861 aggcggcccg ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg gttaatcatc
    46921 tggtagaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat ttccctgatg
    46981 aagagatcga tttcctctcc gacggcaaaa ttgaagccca gcttaatgac gtgatggccg
    47041 atctcgatgc agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa gggatgaaag
    47101 tagtgattgc cggacgtcct aatgccggta aatcgagcct gttaaacgcg ctggcgggcc
    47161 gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg tgacgtgctg cgtgagcata
    47221 tccacattga cggaatgccg ctgcatatca tcgacactgc cgggctacgt gaggccagtg
    47281 acgaagtgga acgtattggt atcgagcgcg cgtggcagga aattgaacag gccgaccgcg
    47341 tgctgtttat ggtcgatggc accacgacag acgccgttga tccggcagag atctggccgg
    47401 aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt ggtacgcaat aaagccgata
    47461 tcaccggcga aacgctggga atgagtgaag tgaacggtca cgcgttaatt cgtctctcgg
    47521 caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct caaacagagc atgggctttg
    47581 acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca cttacaggcg ctggaacagg
    47641 cagcggagca tttgcagcaa ggtaaagcgc aactgttggg agcctgggca ggtgaactgc
    47701 tggcggaaga gttgcgcctg gcacagcaga acttaagcga aatcaccggg gaatttactt
    47761 cagacgacct gctggggcgg attttctcca gcttctgtat tggtaagtaa ccgcgcttac
    47821 gaagccgtat tctgactgtc agatgcggct tcgcttcatt gttagcactc ctgttatgcc
    47881 tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa aaaagcattt
    47941 aatactgctc cccgaacgat tgtgattcga ttcacattta aacaatttca gaatagacaa
    48001 aa
//
LOCUS       DAJTRM010000020        47506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRM010000020 DAJTRM010000000
VERSION     DAJTRM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.020"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..532)
                     /gene="tdk"
                     /locus_tag="OO322_001234"
     CDS             complement(<1..532)
                     /gene="tdk"
                     /locus_tag="OO322_001234"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5906185.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNER"
     gene            1137..1550
                     /gene="hns"
                     /locus_tag="OO322_001235"
     CDS             1137..1550
                     /gene="hns"
                     /locus_tag="OO322_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5906186.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1694..2602)
                     /gene="galU"
                     /locus_tag="OO322_001236"
     CDS             complement(1694..2602)
                     /gene="galU"
                     /locus_tag="OO322_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5906187.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2804..3817)
                     /gene="rssB"
                     /locus_tag="OO322_001237"
     CDS             complement(2804..3817)
                     /gene="rssB"
                     /locus_tag="OO322_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5906188.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3909..4814)
                     /gene="rssA"
                     /locus_tag="OO322_001238"
     CDS             complement(3909..4814)
                     /gene="rssA"
                     /locus_tag="OO322_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5906189.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4927..5385
                     /locus_tag="OO322_001239"
     CDS             4927..5385
                     /locus_tag="OO322_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5906190.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5435..6277
                     /gene="purU"
                     /locus_tag="OO322_001240"
     CDS             5435..6277
                     /gene="purU"
                     /locus_tag="OO322_001240"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5906191.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6437..6521
                     /locus_tag="OO322_001241"
     tRNA            6437..6521
                     /locus_tag="OO322_001241"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6471..6473,aa:Tyr,seq:gta)
     gene            6568..6699
                     /locus_tag="OO322_001242"
     ncRNA           6568..6699
                     /ncRNA_class="other"
                     /locus_tag="OO322_001242"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6883..7560)
                     /gene="narI"
                     /locus_tag="OO322_001243"
     CDS             complement(6883..7560)
                     /gene="narI"
                     /locus_tag="OO322_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5906192.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7560..8270)
                     /gene="narJ"
                     /locus_tag="OO322_001244"
     CDS             complement(7560..8270)
                     /gene="narJ"
                     /locus_tag="OO322_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5906193.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8267..9805)
                     /gene="narH"
                     /locus_tag="OO322_001245"
     CDS             complement(8267..9805)
                     /gene="narH"
                     /locus_tag="OO322_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5906194.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9802..13545)
                     /locus_tag="OO322_001246"
     CDS             complement(9802..13545)
                     /locus_tag="OO322_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5906195.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(13938..15329)
                     /gene="narK"
                     /locus_tag="OO322_001247"
     CDS             complement(13938..15329)
                     /gene="narK"
                     /locus_tag="OO322_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5906196.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15668..17464
                     /gene="narX"
                     /locus_tag="OO322_001248"
     CDS             15668..17464
                     /gene="narX"
                     /locus_tag="OO322_001248"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5906197.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17457..18107
                     /gene="narL"
                     /locus_tag="OO322_001249"
     CDS             17457..18107
                     /gene="narL"
                     /locus_tag="OO322_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5906198.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18108..19502)
                     /gene="ychO"
                     /locus_tag="OO322_001250"
     CDS             complement(18108..19502)
                     /gene="ychO"
                     /locus_tag="OO322_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5906199.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19687..20040
                     /gene="ychN"
                     /locus_tag="OO322_001251"
     CDS             19687..20040
                     /gene="ychN"
                     /locus_tag="OO322_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5906200.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20084..20779)
                     /gene="chaC"
                     /locus_tag="OO322_001252"
     CDS             complement(20084..20779)
                     /gene="chaC"
                     /locus_tag="OO322_001252"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5906201.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(20937..21167)
                     /gene="chaB"
                     /locus_tag="OO322_001253"
     CDS             complement(20937..21167)
                     /gene="chaB"
                     /locus_tag="OO322_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5906202.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21437..22537
                     /gene="chaA"
                     /locus_tag="OO322_001254"
     CDS             21437..22537
                     /gene="chaA"
                     /locus_tag="OO322_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5906203.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            22942..23049
                     /locus_tag="OO322_001255"
     CDS             22942..23049
                     /locus_tag="OO322_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5906204.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23198..24052)
                     /gene="kdsA"
                     /locus_tag="OO322_001256"
     CDS             complement(23198..24052)
                     /gene="kdsA"
                     /locus_tag="OO322_001256"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5906205.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24088..24897)
                     /locus_tag="OO322_001257"
     CDS             complement(24088..24897)
                     /locus_tag="OO322_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5906206.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(24901..25293)
                     /locus_tag="OO322_001258"
     CDS             complement(24901..25293)
                     /locus_tag="OO322_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5906207.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25290..26123)
                     /gene="prmC"
                     /locus_tag="OO322_001259"
     CDS             complement(25290..26123)
                     /gene="prmC"
                     /locus_tag="OO322_001259"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5906208.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26123..27205)
                     /gene="prfA"
                     /locus_tag="OO322_001260"
     CDS             complement(26123..27205)
                     /gene="prfA"
                     /locus_tag="OO322_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5906209.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27247..28503)
                     /gene="hemA"
                     /locus_tag="OO322_001261"
     CDS             complement(27247..28503)
                     /gene="hemA"
                     /locus_tag="OO322_001261"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5906210.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            28717..29340
                     /gene="lolB"
                     /locus_tag="OO322_001262"
     CDS             28717..29340
                     /gene="lolB"
                     /locus_tag="OO322_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5906211.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29340..30191
                     /gene="ispE"
                     /locus_tag="OO322_001263"
     CDS             29340..30191
                     /gene="ispE"
                     /locus_tag="OO322_001263"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5906212.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30342..31289
                     /gene="prs"
                     /locus_tag="OO322_001264"
     CDS             30342..31289
                     /gene="prs"
                     /locus_tag="OO322_001264"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5906213.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31414..33093
                     /gene="dauA"
                     /locus_tag="OO322_001265"
     CDS             31414..33093
                     /gene="dauA"
                     /locus_tag="OO322_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5906214.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33148..33426)
                     /gene="ychH"
                     /locus_tag="OO322_001266"
     CDS             complement(33148..33426)
                     /gene="ychH"
                     /locus_tag="OO322_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5906215.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            33704..34288
                     /gene="pth"
                     /locus_tag="OO322_001267"
     CDS             33704..34288
                     /gene="pth"
                     /locus_tag="OO322_001267"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5906216.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34405..35496
                     /gene="ychF"
                     /locus_tag="OO322_001268"
     CDS             34405..35496
                     /gene="ychF"
                     /locus_tag="OO322_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5906217.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36340..39207
                     /locus_tag="OO322_001269"
     CDS             36340..39207
                     /locus_tag="OO322_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5906218.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLSAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39307..41226)
                     /gene="dhaR"
                     /locus_tag="OO322_001270"
     CDS             complement(39307..41226)
                     /gene="dhaR"
                     /locus_tag="OO322_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5906219.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41454..42524
                     /gene="dhaK"
                     /locus_tag="OO322_001271"
     CDS             41454..42524
                     /gene="dhaK"
                     /locus_tag="OO322_001271"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5906220.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDAMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42535..43167
                     /gene="dhaL"
                     /locus_tag="OO322_001272"
     CDS             42535..43167
                     /gene="dhaL"
                     /locus_tag="OO322_001272"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5906221.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43178..44596
                     /gene="dhaM"
                     /locus_tag="OO322_001273"
     CDS             43178..44596
                     /gene="dhaM"
                     /locus_tag="OO322_001273"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5906222.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            44916..46613
                     /gene="treA"
                     /locus_tag="OO322_001274"
     CDS             44916..46613
                     /gene="treA"
                     /locus_tag="OO322_001274"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5906223.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(46692..47131)
                     /locus_tag="OO322_001275"
                     /pseudo
     CDS             complement(46692..47131)
                     /locus_tag="OO322_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11909 a        12240 c        11640 g        11717 t
ORIGIN      
        1 atcgttcatt accaccaatt accacctgct caccttcgtt ataaggtctg cctgcttgat
       61 caagacgcag caccatgctt gctttacggc cacaaaaaca gatggttttt aattcaacca
      121 gtttgtcgga ccatgccagt aagtattggc tgccaataaa taattcacct cgaaaatcgg
      181 tacgtaaacc ataacaaagt acgggtatat cgagttgatc gacaacctcc gataattcat
      241 atacttgttg tctggttaaa aactggcatt catcaaccag tacgcaatga attgcctgct
      301 gttcatgttc cgcacgaatc tcatcaaata atgatgaatt ttggttaaat aattttgcag
      361 gcgatgacaa acctatacgc gaactgactt tcccggcacc aaagcgatca tcaatttctg
      421 ccgtatatac gacagtgcgc atgccgcgtt cctggtaatt gtatgaagat tgcaacaatg
      481 ctgtagactt acccgcattc attgcggaat agtagaaata tagctgtgcc atcagccaca
      541 ggccctcaat gatatgaaat aaagttggct ggagtttatc ataattcgtc agtttttcag
      601 ctcaacagta tgcacagaag tgggtatatg cgttctccct tacgaagcct tgcataatcc
      661 ttctgagcta tcattacaac tgcctcgctt gttataagcg ggtaaatgac tgctggtaac
      721 tattcacaat ctttaacctg ttgcgcaagt aatagccctc tgttgacctc caggagatag
      781 tgcaatacta agtccatgct cttattgcga ctgttctact tttcatcatt cgcttaatag
      841 ggaattctcg taaacacaac taatacagaa gactgaaagg tcgtcagcct acgataatct
      901 ccccataaaa tgtgacatga atcaggaagt tttaacctca cgtgctgcga aatcatcggt
      961 gtaaataggg ctatatgccg cgtcttttct ggctaatttt atgaaaagat atttattggc
     1021 ggcacaaaat aaagaacaat tttgaattcc ttacattcct ggctattgca caactgaatt
     1081 taaggctcta ttattacctc aacaaaccac cccaatataa gtttgagatt actacaatga
     1141 gcgaagcact taaaattctg aacaacatcc gtactcttcg tgcgcaggca agagaatgta
     1201 cacttgaaac gctggaagaa atgctggaaa aattagaagt tgttgttaac gaacgtcgcg
     1261 aagaagaaag cgcggctgct gctgaagttg aagagcgcac tcgtaagctg cagcaatatc
     1321 gcgaaatgct gatcgctgac ggtattgacc cgaacgaact gctgaatagc cttgctgccg
     1381 ttaaatctgg caccaaagct aaacgtgctc agcgtccggc aaaatatagc tacgttgacg
     1441 aaaacggcga aactaaaacc tggactggcc aaggccgtac tccagctgta atcaaaaaag
     1501 caatggatga gcaaggtaaa tccctcgacg atttcctgat caagcaataa tcttttgtag
     1561 attgcacttg cttaaaatcc cgccagcggc gggatttttt attgtccggt ttaagacaat
     1621 ttaataagaa taagctataa aaaaacggcg tcgattgctc aacgccgttt cgtggataac
     1681 accgatacgg atgttacttc ttaatgccca tctcttcttc aagccaggct ttaaattccg
     1741 tgccaagggt gttatgacga ataccgtatt caacgaaggc ctgcatgtaa cctaatttat
     1801 taccgcagtc atggctcttc cctttcatat gataggcttc caccgtttct ttttcgatca
     1861 gcatatcaat tgcgtcggtg agctgaattt catcaccagc tcccggaggg gtttttgcca
     1921 gcaacggcca aatatccgcg ctaagtacgt aacgacccac aatagcgaga ttagacggcg
     1981 caacatccgc tttcggtttt tctaccacac caaccatcgg tacgctttca cccggcgcta
     2041 attcaacgcc tttgcaatcc acaacgccat atgcggtcac atcagcaacc ggttcaacca
     2101 tgatctggct atgacccgtt tcatcaaagc ggcggatcat ctctgccagg ttatcctgtg
     2161 acaaatcgga ttcatattca tccagaataa catcaggcaa aataacagct accggttcat
     2221 cacccactac cgggtgagca cacaataccg cgtgtcccag gcctttcgcc agaccctgac
     2281 gaacttgcat aatagtcacg tgcggtggac aaatagactg cacttcatca agcagttgac
     2341 gttttacacg tttttccagc attgcttcca gttcaaaact ggtatcaaag tggttttcaa
     2401 tagagttttt agatgagtgt gtaaccagca caatttcagt aatgccagcc gcaatacatt
     2461 cattcacgac gtattgaatt aatggcttat cgacaagtgg cagcatctct ttcgggatgg
     2521 ctttcgtcgc cggcaacatc ctggttccta atcccgcaac ggggataacg gcttttttga
     2581 ctttcgtatt aatggcagcc atttaaattc tcctggactg ttcgtgtttt gaacgtgttc
     2641 atatttctgt atcgcatccc agtatatcag caccccgaag gggctaagtt ctgaaaagga
     2701 ggcgagatag cataattaag acaattactt atacgaatgg cgacgatagt accaccagcc
     2761 gacattagca ggtaatgcaa atttagcccg cgttatcgtt tgctcattct gcagacaaca
     2821 tcaagcgcag tcgaccaccg gttccccata tttggcattg ccaggcatcg catcgctggc
     2881 tcaattgatt caaataagcg ttacctaaag tgcctaacgg aacaccatta ctgatttgca
     2941 cctggtgttc gccggtattt aacgtcgcat tcagacccgc agaaaccaga atgagatttt
     3001 tcagttcgcg atgataatag ccaactaata gcggaaactg ccccggcaga ttggcctgac
     3061 gaagtaaatg gtttacctgc ttcaataacg cgcccaactc tggcaaccgt tgattttggt
     3121 gtgcaagctg ttcctgtaat aatccgttaa acaatgcgcg taataacaag gcagcaagta
     3181 cgccattatg tccagctcgg gtgacatcaa ggcaataaaa tgccagatcg ttttccgaaa
     3241 gtgcggcaat atcaagcacc aggccgggtt tgtccgcggc aaccaattga cggtaattaa
     3301 cccggcaatg ggaaatcacc tgctgaaccg gcggttgtag ttcctgtaat aatttcgccg
     3361 ctgcggcagg gttatcaacc attgcatccc agtcgcgaaa aagcctttcc tcttcctcaa
     3421 cgcgcgaatt aaacatgctg ggatagagac aggcaaaaac catctcgcgc aagcgattca
     3481 gatctttaac tggtttcagc aaaacatctt caacgcccag acgtaacgct ttggcaatat
     3541 ctgccatatt ttcagtggca gatatcacca gaactggggt ctggtcgcct ctgttacgta
     3601 tatgctccag cagtttaagc ccgttcattc gtggcatcgc gatatcacat atcatcaggt
     3661 ctggagtgaa acctcccagc aactcaaggg catccacccc atcagccgcc agtaccgttg
     3721 tcgctcccaa tgaggaaaac catgaatcca gaagcgagcg aaatacctgc tcatcttcaa
     3781 caatgagaat ctgttttccg accaatggct gcgtcatgtt ctctcccctg actggcttta
     3841 ctcaatagtg gcatgctatt gccacctgcg cctgtcagaa ttaacttaag tgtagataaa
     3901 aattctggtc aaatgttggt gcgtaccaac ggcaaaagtt cgtccatttt cctttccact
     3961 gccagctgtc cggccgcaat ggcagcgtgc gcgcgatgga aatcaagcgt agatatttgc
     4021 gggcaaacag gttgaatcag aatatcgggc ggatcacctg ccatgcggtt ccttttaagg
     4081 cggttctcca acacctggat agacgtggtc ataatctctg ttgccgttgg cgctgtcaca
     4141 gcccgacgtg tcgttatgct gcctaacctt tctttcagac gcgcatgcca cggtagagaa
     4201 tcaccattct cgctattttc ttcactgaca ttaaaggaga gcagatcttg ttgcatcaaa
     4261 tgagcatcgt gctgcaggtc gaccgctatc acaatatcag cccccaatgc acgcgtaaga
     4321 gaaataggaa ttgggttaac tactgcccca tcaaccagcc agtagccgtt atgtgcaacg
     4381 ggtgccatga gtcctggaat actgcatgat gcgcgaatag caagatggag atcgccttca
     4441 gtaaaccata attcacgccc cgtacttaaa ttggtggcaa cagccgcaaa gcgacgggaa
     4501 caattttcga tctctgtttc cggcattatt tcgcgatatt gattgaagac acgctcgccg
     4561 cgcagtaacc caccgcgctg ccaggagagg tccatcaggc gtaaaacgtc ccaataactg
     4621 aaagaggtta cccaatcttc cagcgcagat aatcgatcgc atgcataggc cgcgcccacc
     4681 agcgaaccaa ttgaacatcc tgcaacgata tcaatttcaa tacccacttt ttttagcgca
     4741 ttaataacgc caatatgcga ccaacctctc gccgcgccag atcccagcgc cagccctatt
     4801 tttatctttc tcattattcc cgcaagattc ccctggaatg caatcgtagc cacatcgcta
     4861 ctgctcggtt aacatagtgc tactcaggcg gttaatacgc cgttattgtt tccagggaga
     4921 ttatttgtgt ctcagctttg tccctgtggt agtgctgtcg agtatagcct atgttgccac
     4981 ccttatgtgt ctggtgaaaa ggttgcacct gatccagaac atctcatgcg ttcgcgttac
     5041 tgcgcttttg tgatgcaaga cgctgattat ttaataaaga cctggcatcc ctcttgtgga
     5101 gcagcggcgt tacgtgccga attgatgacc ggatttgcgc atactgaatg gttgggattg
     5161 accgtatttg aacattgttg gcaggacgcc gacaatatcg gatttgtcag ttttgttgct
     5221 cgatttaccg aaggtggcaa aacaggtgca ataattgaac gttcgcgatt tttgaaagaa
     5281 aacggtcaat ggtactatat tgacggtaca cgtccgcagt ttggtcgcaa cgatccctgc
     5341 ccttgtggtt caggtaaaaa atttaaaaag tgctgcggcc aataatggtt gacggtacgg
     5401 tttagcaaac actctcaaca aggtttttcc agcaatgcat tcactccaac gtaaagttct
     5461 gcgtactatt tgtccggacc aaaaaggtct gatcgcacgt attaccaata tttgctacaa
     5521 gcacgagtta aatatcgtac agaacaatga atttgttgat caccgtaccg ggcgcttttt
     5581 tatgcgcacg gaactggaag ggatttttaa tgattccacc ctgctggcgg atctcgatag
     5641 cgcattgcca gaaggctccg tgcgtgagct gaatcctgcc ggtcgtcgcc ggatagtgat
     5701 tctggtcact aaagaagcgc attgccttgg cgatttgttg atgaaagcca attacggcgg
     5761 tctggatgtc gaaatcgcgg cagttattgg taaccacgat actttacgtt ctctggttga
     5821 gcgttttgat attccgtttg agctggtaag ccatgaaggg ttaagccgca acgagcacga
     5881 tcaaaagatg gcggacgcca ttgatgctta tcaacctgac tatgtggtgc tggcgaagta
     5941 tatgcgggta ttaacgccgg aatttgtggc acgcttcccg aataaaatca tcaatattca
     6001 ccattcattc ctgcctgcgt ttatcggcgc acgtccttat caccaggcct atgaacgtgg
     6061 tgtgaagatt attggcgcaa ccgctcacta tgtgaatgac aatctggacg aaggcccaat
     6121 catcatgcag gacgttattc atgtcgatca tacctacaca gctgaagata tgatgcgcgc
     6181 aggtcgtgac gtcgagaaaa acgtcttaag tcgtgcgcta tacaaagtac tggcgcaacg
     6241 cgtctttgtt tacggtaatc ggacgattat tctttaatcg ccagcaaaaa taactggtta
     6301 cctttaatcc gttacggatg aaaattacgc aaccagttca tttttctcaa cgtaacactt
     6361 tacagcggcg cgtcatttga tatgatgcgc cccgcttccc gataagggag caggctagta
     6421 aaaagcatta ccccgtggtg gggttcccga gcggccaaag ggagcagact gtaaatctgc
     6481 cgtcatcgac ttcgaaggtt cgaatccttc ccccaccacc atcactttca aaagccccgg
     6541 aattctcaaa cgaatctgca atcaaatatt ctgcccaagc ggggaaggat gagaagcttc
     6601 gaccaaggtt cgacttgagc gccagcgaga gagcgttgcc gcaggcaacg acccgaaggg
     6661 cgaagcgcgc agcgctgagt aatccttccc ccaccaccat cgcttccttg aataatcaaa
     6721 acctgttact ccgagctaat tttgcccgat ggcaccacgt ttaccgggcc tacaatatcc
     6781 tcccctgtag gtcggataag cgaagcgcat ccgacaactc accccgcacc gtgcccataa
     6841 aaaaaccccg ccgaagcagg gtctatgtga actaaaattc gtttagtgac gagcgcgcac
     6901 cagctggtac ttacgtgtca gatactccac cggtacgctc cagatgtgta ccagacgcga
     6961 gaacgggaac agcaggaata acgtcatccc cagcaccagg tgcagacgga agataaacgc
     7021 cacaccatcg aggtgttgag aagcgccacc gtggaaggtc accaccgact gcgcccagcc
     7081 aaccagtttc atcatctcgc taccgtccat atgctgagcg gagaacggaa tggtcagcag
     7141 gcccagcgcg cactggataa cgagcagcga caggatcagg atatccgctc cggtagtggt
     7201 tgcacgcacg cgtgggctga acatacgacg tttcagcaaa agcacgccgc caatcagaca
     7261 cagcacgccg ctggcaccac cagcaaacat cgccattttc tgtttcactt caatcggcag
     7321 ccaggcttca tacatccagt gcggcgtcaa cataccgaag aagtgaccga caaaaatccc
     7381 cagaataccg atatggaaca ggttcgacgc caggttcatc cctttgcgat ccagcatctg
     7441 gctggacgcc gcgcgccagg tgtactgccc gtagtcataa cgcagccagc taccaatcag
     7501 gaagaccgcc ccggctatgt acgggtagat atcaaagaag aacatattca ggaattgcat
     7561 tagtgctgtc ctccggtggt gatattcaga tattgcggcg cgacggcacc ggcaaagcga
     7621 cgctgatgag cagtgattgc tgaatcaccg cagcctttgt cagcaaagaa tttaacctgc
     7681 tcttcttccc agacagcatc cagcgcctgc ggcgtatcat cgcgcgcttc gtcggcaatt
     7741 ttttccgcca ctttgtcgct gtcgatagcg gtattggcca atttcagcag cagatcaaac
     7801 atcacggcat aacggctttc acgctgttgc agacgcgcgc tcagcaatgc cagaatcggc
     7861 gcgatatctt tcaaaccttc cacggcttcg ctttgcggca gctgcgacag gtactccaga
     7921 tacagcggca gatggtccgg taattcgcgg ctgtttaact gcaagccgtg ctgctcgtac
     7981 tgcgccagca ggtccaccat cgcctggccg cggtcacggg attcaccgtg aacatgttca
     8041 aatagcaaca gtgacgtggc acggccacgg tcgaacagtt cgctgtactg gacctgggca
     8101 tcgagcggat ccatcgccgt taaatcgcgc aggaaaatgc ccagcgcatg ggcatcttct
     8161 ttcgacagat ttttcgacgc agcaatcgcc tcaaacatct cttgttgatg ctgccataag
     8221 gcagcatccg gatattcaag gagacgcgat acaatcacga gttcgatcat ggatgcggct
     8281 ccgttttgct ggtcacatcg atggcatcga tacgacggct gttgaacaga ttgaatttgg
     8341 tatctgagcc gtggcagcca tcaccaaagg taaagccgca gccatttttc tccgggaagg
     8401 cttcccgtgc cagttcacga tgactactcg gcaccacaaa gcgatcttcg tagttagcaa
     8461 tcgccagata acggtacatc tcctgtgcct gggcttcggt cagaccgacc tcttccagcg
     8521 cacgggtatc aactttaccg tcaacagttt cagcacgttt gtaatgacgc atcgccagca
     8581 tacgtttcag cgcacgcagt accggtttgg tatcaccggc ggtcagcaga ttcgccagat
     8641 actgtaccgg aatacgcaag ctttcgacgt ctggcagaat tccgttgctg cccagctcgc
     8701 ccgcgtctgc tgcagactga atcggagaca gaggcggcac gtaccagacc atcggcagtg
     8761 tgcgatattc tggatgcagc ggcagcgcca gtttccattc catcgccatt ttataaaccg
     8821 gcgactgctg tgcagcttca ataacgctca gcggaatacc gtctttaatc gcctgctcga
     8881 tgactttcgg atcgttcgga tcgaggaaca cgtccagctg acgctggtag agatctttct
     8941 cgttctcggt gctggctgca cgttcaatcg cgtcggcatc gtacagcagc acgccaagat
     9001 aacggatacg accgacacag gtttctgagc acacggtcgg ctgacccgct tcaatacgcg
     9061 gatagcagaa gatgcacttc tcagacttac cgctcttcca gttgaagtag atttttttgt
     9121 acgggcatcc ggtgatgcac atacgccagc cacggcattt atcctggtcg atcaggacga
     9181 tgccatcttc ttcacgcttg taaatcgcac cgctcgggca ggtcgccaca catgccgggt
     9241 tcaggcagtg ttcgcacagg cgcggcaaat acatcataaa ggtgttttcg aactggctat
     9301 acatcgcctt ctggatgttg tcgaagttct tgtctttcgc cagtttatca aactcaccgc
     9361 ccaggtcatc ttcccagttc ggcccttttt cgattttcgc catccgttcc ccggtaatca
     9421 gcgaacgcgg acgggcaatc ggctgcgatt tgctgccttc cggcgcggta tgcaggttct
     9481 gataatcaaa atcgaacggc tcgtaataat cgtcgatccc cggcagatgc gggttagcaa
     9541 agattttacc cagcagcatg gctcggttac ccatgcgcgg ttgcagtttg ccattgattt
     9601 tacggatcca gccgcctttg tatttttcct ggttttccca gtcagtcggg aagccctggc
     9661 ccggcttggt ttccacgttg ttgaaccacg cgtattccac gccttcacgg ctggtccaga
     9721 cgtttttaca ggtaactgaa caggtgtggc agccgatgca cttatcgaga ttcagcacca
     9781 tgccgacttg tgaacgaatt ttcattttac gctctcctgt acctggtcat tgccttcgcc
     9841 atctaaccag tcaatgttct tcatcttacg cactacaaca aactcatcgc ggttagaacc
     9901 tacggtgccg tagtagttaa agccgtatgc cagatgggca tagccgccga tcatatgcgt
     9961 cggtttcggc gtgatacggg tgaccgagtt atggatacca ccacgctgtt gggtaatttc
    10021 cgaaccaggc aggttaacga tacgttcctg cgcgtggtac atcatggtca tccctgccgg
    10081 aacacgctgg ctgacaaccg cacgggcagt cagtgcaccg ttgctgttga agacttcaat
    10141 ccagtcgtta tcggcgatac ccagatcttt ggcatcggct tcactcaacc agaccaccgg
    10201 accaccgcga cctaaagtca gcatcagcag gttgtcgctg taggtggagt ggatacccca
    10261 cttctggtgc ggcgtcagga agttgagcgc tttttccggg ttgccgttgg atttctggcc
    10321 tatcacttct ttcaccgaac gggtgtcgat cggcggacga taaaccagca ggctttcacc
    10381 gaaatcacgc atccactggt gatcctgata cagttgctga cgaccagaga gcgtacgcca
    10441 tgggatcagc tcgtgaacgt tggtgtaacc ggcgttgtaa gaaacgtgtt catcttccag
    10501 accagaccag gtcgggctgg agataatttt gcgcggctgt gcctgaatat cgcggaagcg
    10561 gatcttctcg tcttctttat tcagcgccag atgcgtatgg tcacgaccgg taaattcgct
    10621 cagggcagcc caggctttca cggctacctg accgttggtt tccggtgcca gcgtcaggat
    10681 catctctgcc gcatcaattg cggtgttcag catcggctgg ccttttgccg gaccttccgc
    10741 tttggtgtag ttgagcttac gcagcagatc catctcgctc tgggtgttcc aggcaatccc
    10801 tttaccgccg ttaccgattt tctccatcag cgggccgata gaggtaaagc gttcgtaagt
    10861 tgccggataa tcgcgctcta cgaccataat gtgcggtgca gttttgcctg ggatcaggtc
    10921 gcattcgcct tttttccagt ctttcacatc cagcggctgc gccagttcag cggcagagtc
    10981 gtgctggata ggcagcgtga cgatgtcggt ttctttaccc agatagccga cgcacacttc
    11041 ggagaatttc ttcgcgatgg ctttgtagat ttcccagtcg cttttcgctt cccaggccgg
    11101 atcgaccgcc gcagacagcg ggtgaataaa cggatgcata tccgaagtat tcatgtcgtc
    11161 tttttcgtac caggtcgccg tcggcaaaat gatgtcggaa tagagacagg tgctcgacag
    11221 acggaagtcc agcgtaacca ccagatccag cttgccttcc agaccattgt cctgccagtc
    11281 cacttcttcc ggcttcacgc cgccctgttg ccccagatct ttaccctgga taccgtgctc
    11341 cgtccccagc aggtacttga gcataaactc atgaccttta ccggaagaac cgagcaggtt
    11401 agaacgccag atgaacaggt tgcgcgggtg gtttttaccg ttttctggtt gttctgccgc
    11461 aaaacggatg gaaccctctt tcagggattt caccgtatag tccaccggat tcatcccggc
    11521 tttttccgct tcgccagcga tagtcagcgg gttagtgcct aactgcggtg cagacggcag
    11581 ccagcccatg cgttccgcac ggacgttaaa gtcgatcaag tgtccggtat agcgggattt
    11641 gtccgccatc ggtgacagca actcttccgc cgtgacggtt tcataacgcc actggctgga
    11701 gtggttatag aaataagaag tgctgttcat gtgacgcgcc ggacgctgcc agtcaagggc
    11761 aaacgccagc ggctgccagc cggtttgcgg acgcagtttt tcctgaccta catagtgcgc
    11821 ccagccgccc ccgctctgac cgacacagcc gcagaaaatc agcatgttga tcagaccacg
    11881 atagttcata tcgaggtgat accagtggtt cagccccgca ccgacgataa tcatcgaacg
    11941 accgtgcgtt ttatcagcgt tatcggcaaa ttcacgggcg atgcgaataa tctggctgcg
    12001 agaaacgccg gtaatctgct cggcccaggc cggggtataa gctttcacat cgtcatagct
    12061 ggttgcacag ttaacgtcgt tcaggccacg ttccagaccg tagtttgcca gcgtcagatc
    12121 ataaacggtg gtcaccaggg cggtgctgcc atcagccagt tgcaggcgtt tcaccggcag
    12181 tttgtgcagc agcacgtttt ccagttccac tttgttgaag tgttccgtgc cgtcgccacc
    12241 aaagtacggg aagcctacct cagcgatctc atcctggcta cccagcaggc tcagttgcag
    12301 ctcggtttct tcgccagttt tgccgtcgcg ctgctcaaga ttccatttgc ccttctcgcc
    12361 ccagcggaag ccaatagaac cgttcggcgc aaccatttcg ccattggtat taaaggcgac
    12421 agttttccat tccggattgt tttcctggcc cagcgcatca accagatcag cagcgcgcag
    12481 catacgacct gcagcgtagt aaccgtcgcg ttcttccagc atcaccagca tcggcatgtc
    12541 ggtgtagcga cgcacatagt cggtgaaata ctggcttggg ttgtcgaggt ggaattcacg
    12601 cagcattacg tggcccatcg ccagcgccat tgccgcatcg gtgccctgtt tcggtgccag
    12661 ccacagatcg cacagtttgg cgatttcagc gtagtctggt gtgacggcaa cagttttggt
    12721 gcctttgtaa cgcacttcag taaagaagtg agcatccggg gtacgcgtct gcggcacgtt
    12781 tgaaccccag gcgatgatgt agctggagtt ataccagtca gcagattccg gtacgtcagt
    12841 ttgctcgccc caggtttgcg gagacgcagg aggcaggtcg cagtaccagt cgtagaagct
    12901 taagcaagta ccgccaatca gcgagagata gcgtgcaccc gatgcgtaag aaaccatcga
    12961 cattgccgga attggcgaga aaccagcaac acggtccggg ccgtagtttt tgatggtgta
    13021 aacgttagat gcggcgatca gttcgttcac ctcctgccaa gaagaacgaa caaatccacc
    13081 gcgtccacgc gcctgcttaa agcttttcgc tttatcggcg tcttcaatga tagaagccca
    13141 tgcctcaacc ggatcgctat gcagcgcctt cgcttcacgc cacattttca tcaggcgttt
    13201 gcgcatcatc gggtatttca ggcggttggc actgtaaaga taccaggagt agctggcacc
    13261 gcgcgggcag ccgcgaggtt catggtttgg cagatccgga cgggtacgcg gatagtcagt
    13321 ctgctgggtt tcccaggtga ccagaccgtt tttgacgtag attttccagc tgcaggagcc
    13381 ggtgcagttt accccgtggg tagagcggac gattttgtca tgctgccaac gctggcgata
    13441 tccatcctcc cagtcacggt tggtattgag aagctggcca tgcccatcgg caaaggtttc
    13501 acccttctgt ttgaagtagc gaaaccggtc caggaattta ctcatcggtt ttctcctgtg
    13561 ggagcctgtc ggccctctga taaatcgaca ttgctaatgt ttaagggcaa ggtaacgctc
    13621 tgaaacagcg tgggaattga taacgatcaa gagtgatggg gatgaaaaat aaagtaaatt
    13681 cctaagatac ccctttagga taattaacag ttacagcata acatgctgta aattaagacg
    13741 ttttatacct atagttattt aaactattaa gatttttatg caaatgggta ttaaggagta
    13801 ttccccatgg gtaacatatt aatggctctt aatgaaatcc ggcgaaataa gccatataac
    13861 actaatatgt tgtaactaaa tgacgcataa aaaaagcgcg gcatcatgcc gcgcaaagga
    13921 taatcaaata ttaagattta ttttttagaa tgccgaccat ataccgccca agtaatcact
    13981 acgcaggcga tatagaaaat caaaaatact ttcattgcac ctactggcga acccgttaat
    14041 gccagcgagc taccaaacgc tttcgggata aagaagccac caatcgcgcc aatcgcagag
    14101 atgaaaccca gcgccgctgc cgtgccggtt gccgcttcac gcatcgcacg ttcgtcagaa
    14161 cccccttctg ctttcacgcg atccattgtc agtttacgga agatcactga aatcatctgg
    14221 aaagtggaac cactacccag cccagctgtc aggaacagcg ccaggaagac cgcgaagaac
    14281 gccatgaagc ttccgccctg cccgtcagtc ggtaaggtca ggaacagcag gccgctgaaa
    14341 atcgccatca gaataaagtt caccagcgtg acacgagttc cgcccagacg gtcagataat
    14401 gcaccacctg cagaacgcgc cagcgcacca ataaacggcc cgaagaaagc gtattgcaga
    14461 atctgaacat ccgggaactg cgtttttgac agcatcgcaa agcccgcgga gaagccgatg
    14521 aaggagccga aggttgccag atacagcagg ctcataatcc acagatgacc ccgtttgagt
    14581 accggcaact gctccttgat ggaggctttc gaggtagcaa gatcgttcat gccaaaccac
    14641 gccgcaatgg tgaagatggc aaggaacggc acccatatcc aggacgcatt cgccagatac
    14701 agctcagtcc catccggctg tttgacgccc tggctaccaa atactgcgaa aatcgacagt
    14761 gataccacca gcggagcaac caactgcatg acgctgacgc ccatgtttcc cagaccacca
    14821 ttcagaccca gcgcgccacc ctgcttctgt ttcggaaaga agaagctgat gtttgccata
    14881 ctggatgcga agttcgcgcc agcaaagccg catagcagag agatgatgat gaagacgcta
    14941 taaggcgtgg aggtatcctg cacggcaaaa cccagccaga cgcaaggaat aatcagaata
    15001 ccggtgctga acgccgtcca gcgacgacca ccgaagatag gaaccataaa ggagtatgga
    15061 acacgtaata acgcgccaga aaccgaaggc agcgcagtca acataaatag ctgatcggtc
    15121 gtaaaattaa aaccgacttt cggtaagttc accgcaacag cgctgaacaa catccatacg
    15181 caaaacgcca gcagcagaca gggaacggaa atccacaggt tgcggctggc aatacgttga
    15241 ccgcgttgtt gccagaacgc aggatcttcc ggtcgccaat ctgttatgac agctccagta
    15301 gccctttcgg aggcagatga gtgactcata gacacctctg atactcgttt cgatgtctgc
    15361 caccttagtg tctggagcta aaggcaattt gatgtaaatc aaacgataag ataactttat
    15421 ctatcattga tatttatcat tacccatagt gagtacagtg atttcataaa aattatgaga
    15481 tttttcacgg tgctgtagaa tccctaccct taccgatgta aagcgactaa ccacacggca
    15541 aataaggagt aactctttcc ggctatgggt atacttcagc caatagccga gaatactgcc
    15601 attccagaat atatcgtcac attcattaag gttattgctc atttaaagcc tgaaggaagg
    15661 ggtttacatg cttaaacgtt gtctctctcc gctcaccctg gttaatcagg ttgcgcttat
    15721 tgtgttgctt tctactgcta ttggactggc agggatggcg gtttctggct ggctggtgca
    15781 aggcgttcag ggcagcgccc atgcgatcaa caaagcggga tcgctgcgca tgcaaagtta
    15841 ccgtctgttg gcggcagtgc cattaagcga gaaagacaag cccttaatta aagagatgga
    15901 acaaacggca tttagcgccg agttgactcg agcagcagaa cgagacggac aactggcgca
    15961 attacagggc ttacaagatt actggcgtaa tgaactgatc cctgcgctga tgcgtgcaca
    16021 aaaccgagaa acggtgtcag cggatgtcag ccagtttgtt gccgggcttg atcagctggt
    16081 atctggtttt gaccgcacca cggaaatgcg tattgagaca gtggtactgg tccatcgggt
    16141 aatggcggta tttatggcac ttttactggt gttcactatt atctggttgc gggcgcgact
    16201 gctacaaccg tggcggcaac tgctggcaat ggcgagtgcc gtcagccatc gcgattttac
    16261 ccaacgcgca aacatcagcg ggcgcaacga aatggcgatg cttggaactg cgttgaacaa
    16321 tatgtctgca gaactggccg aaagttatgc cgtacttgag cagcgggttc aggagaaaac
    16381 cgccgggctg gagcataaaa atcagatcct ctctttttta tggcaggcta accgccgttt
    16441 gcattcccgc gccccgctgt gtgaacgcct gtcaccggta ctcaacggct tacagaattt
    16501 aaccctgcta cgtgatatcg aactgcgggt gtatgacact gatgatgaag agaatcatca
    16561 ggagtttacc tgccagccag atatgacttg tgatgataaa ggctgccagc tctgcccgcg
    16621 cggcgtatta cccgttggtg atcgcggcac gaccctgaag tggcggctgg ctgactctca
    16681 tacgcagtac ggtattttgc tggcgaccct gccgcagggg cgtcatctta gccatgacca
    16741 acaacaactg gtggataccc tggttgaaca acttaccgcc acgctggcgc tggatcgcca
    16801 tcaggaacgt cagcaacagt tgatcgtgat ggaagagcgt gccaccattg cgcgcgaact
    16861 gcatgattct attgcccaat ctctctcttg catgaagatg caggtgagtt gtttacagat
    16921 gcagggcgat gcgctgccag aaagcagccg cgaactgtta agtcagatcc gtaacgaact
    16981 gaatgcatcc tgggcgcagt tgcgtgaatt gctcaccaca ttccgcttgc agctcaccga
    17041 gcctggatta cgtccggcgc tggaagcgag ttgcgaagag tacagcgcca aatttggctt
    17101 cccggtgaag ctggattatc aattgccgcc tcgtctggtg ccttcacatc aggcaatcca
    17161 cttgttgcaa attgcccgtg aggcattaag taacgccctc aaacattcgc aagcgagtga
    17221 ggtcgtggtg acggtggcgc aaaacgataa tcaggtcaaa ctgaccgtcc aggataacgg
    17281 ctgtggcgtg cctgaaaatg ccatccgcag caatcactac ggcatgataa taatgcgcga
    17341 ccgtgcgcaa agtttacgag gcgattgccg cgtccgccgt cgtgaatcag gtggcaccga
    17401 agtggtggtc acctttattc ccgaaaaaac tttcacagac gtccaaggag atacccatga
    17461 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
    17521 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
    17581 aacagggtat tgaactggcg gaatctcttg atcccgatct gatcctgtta gatctcaata
    17641 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
    17701 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
    17761 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
    17821 aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct
    17881 tgcgcgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca cgcgagcgcg
    17941 atattctcaa gctgattgcc cagggtttgc cgaacaagat gattgcccgc cgcctggata
    18001 tcaccgaaag cacagtaaaa gtgcacgtca agcacatgct gaagaaaatg aagctcaagt
    18061 ctcgcgtgga agcagcggta tgggtgcatc aggagcgcat tttctgatta cggttcccag
    18121 cgcagttcgt cgtttgacaa tgcatcgaac ggttcgacaa gcgttagcgt gatctcattg
    18181 gaggagacac gctgcccctg gttatcttcc accaccacag acaatcgcca gtgattgctt
    18241 gccccttccc cgttctgcca gtcaggcatg atcagcgtcc agccctccgc gctgttggct
    18301 tgtgcgcccg gcgtcaaact taatatctgc gtatcgccct gccaaatcag ttgccgaata
    18361 ccgtaacgac tgcggatttg taatttcagc ggcactgttt cgccaggttt tagatcccac
    18421 ggcggtgtcg ccagaaacac cgttaacgtt tttcgctgtc ggtactcaag agtcggtaga
    18481 ttatttcgct gcggattgtc atagcgacta ccacgtaacg actgactttc ggcaacctcg
    18541 cccgcagaaa gttgtttttt gagcggtaca ccaaagcggt aattaagatt cagcccgagg
    18601 ttattttgat tttcgccact ttccccctgt ttatgctggg ccgtcacagt gactaatggc
    18661 acaggggtgt aatttaatcc cagactcaac gcgacgggat tgtgataacc cgtaccagag
    18721 ttaaacaaat caacacgatc accaaaatac tgttctaggc tgacactggt attgaggtgt
    18781 tgatagaacg gcatgcgcat ccgagctgtc aggtcgtacc cgcgcgccat ccgttgttcc
    18841 tgcgtggctg tctgttcatg ccatgcagca aacggctgat aaaagtttgc cgataatcgc
    18901 aaatattcgc cccacgcttc ggcaccaaag cccgctcgct gaagattttc gtcctgcaag
    18961 ttgtcataaa aagtgttata acccaccagc cagttgccgc gcgcccagcg ttgcccaacg
    19021 cccacattgc tcaccaaccc attatcctgc tgagtaagac caagctggct ccaggtgaga
    19081 taacgatcat tatcttgtaa cggcacaaac cagcttccac gactgccggt gaaatgtcct
    19141 tcgttatcca ctttgacgtc aacactggca tttccccacg gtgatagcca ggactctacg
    19201 tgctgattaa cctgttgact aagcgcgtcg cggacttttc ccaatgcgaa agcttttgcc
    19261 tgctcgccag tatccagccc gttatcattc atactggttt cgccgaaatc tttcacaatt
    19321 tcagcaaagt gtttttcccc atcatgattt tcgggagcca tgcctaaatc cggcagacca
    19381 tcattgttat tatcaaaggg atttgccgct ttttgctcga aggtagattg tgcgttagct
    19441 gtaccgcctg ctacaagcaa gagtaaataa aacgggataa tgcgaggaac gaaacggctc
    19501 aaaacgtcgg taacttccgg taataatgaa aaaccatagc cttaaagaat aacgattact
    19561 ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
    19621 gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag
    19681 taaataatgc aaaaaatagt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
    19741 tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt
    19801 ctgttcctga tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc
    19861 tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc
    19921 aaaacctgta ccgacgggcg cgggattagc acacttcctc tgattgatgg ggtggaaatc
    19981 ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa
    20041 tgtttaaggg ggcgtctgac gccccactat gagggaagtt tactcaggcg aatcccggcc
    20101 ttaacacacc atccggataa ttctccgcca gtagtttttt taccgatacc agcaaatcat
    20161 tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt
    20221 gtgcattggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg acctgagcgc
    20281 gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg
    20341 tgcgtccatc atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct
    20401 cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggtagt cgataggcga
    20461 cgcctgtggt gcgtccgccc tctttcagtg caagcatccg tcccggctgg tgcgcagtcc
    20521 cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc
    20581 atgattcggt aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca
    20641 ccggtccctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgttctg
    20701 ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca
    20761 agaaatcacg cgttatcacc ttacacctcc gccactgcat acttccctgc gacaactttt
    20821 tggtgtcttc cggacatttt taccgctcat tttgaaacca ctaacggaac aatatgcaat
    20881 ccacggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggttttac
    20941 gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag
    21001 gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcttc tttatcttta
    21061 tattgatccc atgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggtaga
    21121 acgtgcttta cgctttccgg cagatcgctt ttcgttttat acggcatcgt tgactccttt
    21181 tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt
    21241 tcatgtcaga ctgttcttaa gaaacgctaa aacaaacatt gtctaaacgt aatacttatt
    21301 tttatgcccc aatgagtaaa aaatcgacag cggggtaaaa attcgtaaaa tgttaccgat
    21361 atccagacac tttctgttat ttttcctacc tattttttac attttcgata aattcatctg
    21421 caaagggata accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac
    21481 ttcgcttatt ttcccggttc tggtgctggt agtgctgttc ctgtggggaa gtagccagac
    21541 actaccagtg gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt
    21601 tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct
    21661 tattcttagc ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac
    21721 cggcgacgcc gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac
    21781 cggtgggctg gttggctttt cattattgtt gggcggtcgt aagtttgcca cccaatatat
    21841 gaatctgttt ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact
    21901 ggtatttcca atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt tactggtagc
    21961 attaatttct gcggcaatgt atggcgtatt tttgctgatc caggccaaaa cgcatcaaag
    22021 tttgtttgtc tacgagcacg aagatgacag tgatgacgac gacccgcacc acggtaaaac
    22081 gtctgcccat agcagcctgt ggcatgctat ctggttgatt atccatctga ttgccgttat
    22141 tgcagtcacc aaaatgaacg ccagctcgct ggagacattg ctcgacagca tgaatgcccc
    22201 tgttgccttt actggcttcc tggtggcact gttgattctg tcgccggaag gtttaggtgc
    22261 gttaaaagca gtgttgaaca accaggttca gcgcgcgatg aatctgttct ttggttcagt
    22321 gttagcaaca atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa
    22381 cgaattgcag tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtgct
    22441 gtgccatatc tccttctcca ccggacgtac taacgtgctc aatggcgcag cgcatctggc
    22501 actgtttgcc gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg
    22561 gaaatcggga gagataagat aaattcctgc ttattgtatt tcttcatact acctggacag
    22621 ctcgttaatc agaattgcga gcctgtacca caacctgcac gaaaccactg caacagctgt
    22681 tgaaaagccg gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg
    22741 agacgtagta tccacaccaa gccggaatta tccggtgagg cgcaatgttg cgggggcttt
    22801 atccctggtg gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtaaaacct
    22861 gacaacaaaa cgggggctaa tcttgaaacc agacacttca agaatagccg caaaatgttg
    22921 tcattacaac aaggcggcta tatgacgctc gcgcagtttg ccatgacttt ctggcacgac
    22981 ctggcagcac cgatcctggc ggggattatt gccgcagcga ttgtcagctg gtggcgtaac
    23041 cggaagtaag gtgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg
    23101 ccgcaggggt aacagcctgc ggcctttttt gtatctggca cccatgaaaa aagtcttaac
    23161 gcagaacgct aagactttat ttttcaagca aaaaagatta cttgctggta tccagttctt
    23221 cgaaaccttt caccagatca tcaatcgctt tcatctgctt gaggaacggt tccagtttag
    23281 ccagcggcag cgcggatgga ccatcacatt tcgcatgttc cggatccgga tgcgcttcaa
    23341 taaacagccc cgccagacct accgccatac cggctcgtgc cagctcagcc acctgagcac
    23401 gacgaccacc ggaagctgcg ccaaacggat cgcggcattg cagtgcgtgg gtcacgtcga
    23461 aaatcaccgg cgagttacca gacactttct tcataatgct gaagcccagc atatcgacaa
    23521 ccaggttgtc atagccgaag ttagcaccac gatcgcaaag aatcactttt tcgttgccgc
    23581 cttctttgaa tttatcaacg atattaccca tctgtcccgg gctgacaaac tgtggtttct
    23641 tgacgttaat taccgcaccg gttttcgcca tggcttcaac caggtcagtc tggcgagcaa
    23701 gaaacgccgg caactgaatc acatccacga catcagcaac gggctgtgcc tgacttggtt
    23761 cgtgaacgtc ggtgataatt ttcacgccaa aagtctgctt caactcctgg aagattttca
    23821 tcccttcttc caggcccggt ccacgataag agtggatgga ggagcggttg gctttgtcaa
    23881 aagaggcttt gaacacgtaa gggataccca gtttctgggt cacagttacg tagtgctcgc
    23941 aaatgcgcat cgccagatcg cgagattcca gcacgttcat accgccaaac agtacgaacg
    24001 gcaggtcatt tgctacgttg atgtcgccaa tgctaaccac tttttgtttc ataggatcgc
    24061 cttaatcttg agtaaaatgt cgattaatta atgcagcaca atatgtttat gcgcgatgtt
    24121 atttatttgc gcacggatca tttcgctgat cgggtcttcc ggacaccgtt caacgaaata
    24181 acttaaatcg ttcaacgcaa cgtgttcgca atccagttgc gcataaatca acccacgatc
    24241 gcgaatttca tagggatctt cagggttgaa ttgtaataaa gcttcgctgg tgcgtaacgc
    24301 cagctccatc tgattttctt ccatcaacga ggctttgagt gtatccagca atttgcggat
    24361 tacctcaatg ttatcagctt catcaaggtc ttcataaaac agttccgccg acgggctgat
    24421 atttcccttt aaccatccgt ccagcatatg ttcgcttaac gattcaccgt taaaaggatt
    24481 aatcagccaa atttcgccat ccggacattc aatgcgcaat atcagctgcg tagggaaaat
    24541 caccggcagc agcggcaaat cgagacgatt cgcgacccat aataaaaccg cacctaatga
    24601 taccgcactg ccctgtcgat tctttaacac ctggtccagc cataatgcat cggaaagacg
    24661 ataaacgccg cgtgaggctt taaatcccca gtcgccgtaa aacagcgcga tcagtttttc
    24721 cagctgctct tctaaaggca gaagctggct gatttcttcc ttcgccagac taacgagacg
    24781 ctccagttcg tcgtaaacat cttgcgaggg aaaatcgcgg cggattgctt cgcaagccag
    24841 gatcatgcct tcgcacaatg gcgctttatt aaattcgaaa tcagctaacg atctcatgac
    24901 ttaccccagt aacggtactt ttgtggtggc gagtttaatg atgatgtaca gcaccaccaa
    24961 cgccagcggg aaggcaataa tgcgcgcctg ctgactgtgc atacgacgat aatcgagtgc
    25021 aataaaaccc aaaacgatat aaatgataac tccaaacagc ttttcagtca gccactgtgc
    25081 ctgcccggaa aatggcagga tgtgcgcttt agccatcaac gcaatgccgc ttaacaataa
    25141 caccgtatcg acaaccggcg gcacaatgcg cgtccagcga gcaaatgcct gtggatgctt
    25201 ctgataacgt agccagaaac gtaaggttaa tagcccaaca gaaagcgcga tactaataag
    25261 atgaacacta agcagtgtag aaaaacttgt cattgataat agcggccgag cgttacgcgc
    25321 tcgttatcac catagtcacg gcaggtttcg acgtcatgat accccgcgag gataaatgct
    25381 tgtcgcaccg cttcgccctg ctgccagcca tgttccagaa gcagaaagcc gccggatacc
    25441 agcgcgttac gcgactgttc gatgatatgc acgatgtctg ccattccact gtctgccgca
    25501 accagcgcag tgagcggctc aaagcggaca tcgccttgtt gaagatgtgg gtcctgctcg
    25561 tcaatatacg gcggattgct gacaatcatc gcaaactgct gcccggctag cgcgctaaac
    25621 cagtcgcttt gcagaatgtg gatatttttg atcgccagat gctgggcatt acgttgtgcc
    25681 agggagacag catcaggcat acgatctaca gcgataattt cgcagtccgg gcgctcgcta
    25741 gccagcgcaa gcgcaatcgc cccggtaccc gtcccgagat cgagaatacg gcaaggttgt
    25801 tcaggcaacc gcgccagtgc ctgctccacc agacactccg tatccgggcg cggaattaag
    25861 gtcgctggcg aaacaaataa cggcaacgac cagaattctc gcaccccggt taaatgagca
    25921 atgggttcac catcgcgacg acgtgtcagt agcgcatcaa gttgctgaca ttgttcgtca
    25981 gtcagctgcg tttcaccaaa ggcgagaata aaagtacgcc ctctgccggt aacatgctcc
    26041 agcaggattt cagcatcacg ccgcgggctt tcgctcgcct gaagttggct tattgcttca
    26101 cgtaaccagt gttgatattc cattattcct gctcggacaa cgccgccagt tggtcggcct
    26161 gatgttcctg gataatcggt tcaatcagca tatccagctt accttccatc acttcatcca
    26221 ggcggtagag cgtcaggttg atgcggtgat cggtaacgcg cccctgcggg aaattgtaag
    26281 tacggttacg gtcgctgcga tcgccactcc ccagcaggtt acgacgggta gacgcttcgg
    26341 cctgttggcg ttttgccatt tcagcagcgt ggatgcgagc accgagcact gaaagcgcct
    26401 tcgctttgtt tttatgttgt gaacgttcgt cctgacattc aacaacaatc ccggtcggca
    26461 agtgagtaat acgaattgcc gaatcggtgg tgttaacgtg ctgaccaccc gcccctgacg
    26521 agcggaaagt atcaatgcgt aaatctgctg ggttgatgtc cggcagttct gcgtcaggca
    26581 gttctggcat taccgcaacg gtacaagcag aagtatgaat acgaccctgc gattccgtag
    26641 caggaacacg ttgcacgcga tgaccgccgg attcaaattt cagacgacca tacacaccat
    26701 caccgctaat tttggcgatg atctctttat aaccaccatg ttcaccctcg ctggcgctca
    26761 tgatttctac ccgccagcgg cgggcttcgg cgtaacggct gtacatacgg aacagatcgc
    26821 ctgcaaacag cgctgcttcg tcgccgccgg ttccggcacg gacttcgagg aaggcgttac
    26881 gttcgtcatc aggatctttt ggtagtaata gaacctgtaa ttgctgttcc agttgctcgc
    26941 ttttttcttt agcttcgcgc agttcatcct gcgccatctc acgcatttca ggatcgtcga
    27001 gcatcatctg tgcggtttcg atatcttcct gaacctgttg ccagtcggta aaacagcgcg
    27061 aaacatcact taactgcgca tattcgcgtg ataatgcgcg aaaacgttcc tggtcggcga
    27121 tagtttgcgc gtcacccagc aacgcctgaa cttcttcatg gcgttcatgc agggcttcca
    27181 gtttggcaac gatagaaggc ttcataggcg taaatgcacc ctgtaaaaaa agaaaatgat
    27241 gtactgctac tccagcccga ggctgtcgcg cagaatattc aggcgttcgt tatccccgtc
    27301 acgggcggcc tgttgaagtg atttcgttgg cgcatggatc aagcggttag tcagtttcca
    27361 tgccagatcc tgcataatgg cttgcgcgtc gccgccctgc tcaagggccg ctaacgcttt
    27421 ggcggttaac tcatcgcgaa cgtgctctgc ctggctgcga tactcgcgaa tggtttcgct
    27481 ggcgctttgt gctcgcagcc acgccataaa ttcgctggtt tcctgagcga caatagtttc
    27541 cgcctcaacc gctgcggctt tacgctgcgc caggttgtgc gaaatgatgc tttgcagatc
    27601 gtccacacta taaagataag cattcgccag tttgccaact tccggctcaa catcgcgcgg
    27661 aacggcaata tccaccaaca gcattggttg gttacgacga ctttttaatg cgcgctccac
    27721 catgcctttc ccgataatcg gtaacgggct ggcggtggaa ctgatgatga tatcggcttc
    27781 gcgcagacgt tcgtcgatct cactcagggc aatcacttcc gcgccaactt catctgccag
    27841 aatttgggca cgttcgcgag tgcggttggc gataatcatc ttctgtactt tatgttcgcg
    27901 cagatgacgc gctaccagct cgatggtttc gcccgcgcct accagcaaca ctgtgaccgt
    27961 agagagcgat tcaaagatct gccgcgccag cgtacaagcc gcaaaagcga cagacacagc
    28021 actggcaccg atatctgttt cagtgcgaac gcgtttcgct acagagaaag atttctggaa
    28081 catgcgttcc agttcgctgg ccttcatatg acctttttgc gaatcgacaa acgctttttt
    28141 aacctgaccg aggatctgcg gctccccaag aaccagtgaa tccaggccgc tggcaacacg
    28201 cattaaatgg ctaaccgcgt cgttatcctg atgccagtag aggcttttac gcagatcttc
    28261 ttcattaaga ttgtgataat cgcaaagcca gcggattaac gcctcttgca ggttatcctg
    28321 ctcttcaacg ctaagataaa gttccgtgcg gttgcacgtc gacagcacca cgccgccctg
    28381 caccatcggc tgcgcaagca ggctgtcaag cgcctgatcg agcttatccg gcgaaaacga
    28441 tacacgttct cgcagcgata caggtgccgt tttatggttg ataccgagtg ctaaaagggt
    28501 catgtctgcg ggaaataata ccaacgttga tagggttagt ctgcttgcat catacaggat
    28561 gcgtaagatc aataaaagag agcgccccct tttggagtaa ttgccgaaag ccgttagatt
    28621 ctggcaatta agacaacttg aacatagacg atagcggacg gtaacgctag cattaagggt
    28681 tataactgca acgtatctca aggacttgtc atcactatgc ccctgcccga ttttcgtctt
    28741 atccgcctgc taccgctggc tgctcttgtg ctcactgcct gttccgttac cacgcccaaa
    28801 ggtcctggca aaagcccgga ttcgccacaa tggcgtcagc atcagcaaga cgtgcgcaat
    28861 cttaatcagt atcagactcg cggcgcgttc gcttatattt ctgaccaaca aaaagtgtac
    28921 gcccgctttt tctggcagca aaccggccag gatcgctacc gtctgctgct cactaaccca
    28981 ttgggcagca cggaactgga gctgaatgct caaccgggta acgtgcagtt agtcgacaat
    29041 aaaggtcagc gttataccgc cgatgacgcc gaagagatga ttggcaaatt gaccggaatg
    29101 ccaattccgc tcaacagctt gcgccagtgg attttaggtt taccgggtga tgcaaccgac
    29161 tacaaactgg acgaccagta ccgcctgagc gaaattacct acagccagaa tggcaaaaac
    29221 tggaaggttg tttatggtgg ttatgacacc aaaacgcaac ctgcgatgcc agccaatatg
    29281 gaactcaccg acggtggtca acgcatcaag ttaaaaatgg ataactggat agtgaaataa
    29341 tgcggacaca gtggccctct ccggcaaaac ttaatctgtt tttatacatt accggtcagc
    29401 gtgcggatgg ttaccacacg ctgcaaacgc tgtttcagtt tcttgattac ggcgacacca
    29461 tcagcattga gcttcgtgac gatggggata ttcgtctgtt aacgcccgtt gaaggcgtgg
    29521 aacatgaaga taacctgatc gttcgcgcag cgcggttgtt gatgaaaact gcggcagaca
    29581 gcgggcgtct tccgacggga agcggtgcga atatcagcat tgacaagcgt ttgccgatgg
    29641 gcggcggtct cggcggtggt tcatccaatg ccgcgacggt cctggtggca ttaaatcatc
    29701 tctggcaatg cgggctaagc atggatgagc tggcggaaat ggggctgacg ctgggcgcag
    29761 atgttcctgt ctttgttcgg gggcatgccg cgtttgccga aggcgttggt gaaatactaa
    29821 cgccggtgga tccgccagag aagtggtatc tggtggcgca ccctggtgta agtattccga
    29881 ctccggtgat ttttaaagat cctgaactcc cgcgcaatac gccaaaaagg tcaatagaaa
    29941 cgttgctaaa atgtgaattc agcaatgatt gcgaggttat cgcaagaaaa cgttttcgcg
    30001 aggttgatgc ggtgctttcc tggctgttag aatacgcccc gtcacgcctg actgggacag
    30061 gggcctgtgt ctttgctgaa tttgatacag agtctgaagc ccgccaggtg ctagagcaag
    30121 ccccggaatg gctcaatggc tttgtggcga aaggcgttaa tctttcccca ttgcacagag
    30181 ccatgcttta agccgggcaa gctgagtttc ggtgacaacg tcaccttgtt ccagacgttg
    30241 catcgcgctc tttaatacac cgcctggaaa ggatcatgcc tggcccgcac agttttcggc
    30301 agattctttc caccaatgga cgcatgcctg aggttcttct cgtgcctgat atgaagcttt
    30361 ttgctggtaa cgccaccccg gtactagcac aacgtattgc caaccgcctg tacacttcac
    30421 tcggcgacgc cgctgtaggt cgctttagcg acggcgaagt cagcgtacaa attaacgaaa
    30481 atgtacgcgg tggtgatatt ttcatcatcc agtccacttg tgcccctact aacgacaacc
    30541 tgatggaatt agtcgttatg gttgatgccc tgcgtcgtgc ttccgcaggt cgtatcaccg
    30601 ctgttatccc ctactttggc tatgcgcgcc aggaccgtcg cgtccgttcc gctcgtgtac
    30661 caatcactgc gaaagtggtt gcagacttcc tctccagcgt cggtgttgac cgtgtgctga
    30721 cagtggatct gcacgctgaa cagattcagg gtttcttcga cgttccggtt gataacgtat
    30781 ttggtagccc gatcctgctg gaagacatgc tgcagctgaa tctggataac ccaattgtgg
    30841 tttctccgga catcggcggc gttgtgcgtg cccgcgctat cgctaagctg ctgaacgata
    30901 ccgatatggc aatcatcgac aaacgtcgtc cgcgtgcgaa cgtttcccag gtgatgcata
    30961 tcatcggtga cgttgcaggt cgtgactgcg tactggtcga tgatatgatc gacactggcg
    31021 gtacgctgtg taaagctgct gaagcgctga aagaacgtgg tgctaaacgt gtatttgcgt
    31081 acgcgactca cccgatcttc tctggcaacg cggcgaacaa cctgcgtaac tctgtaattg
    31141 atgaagtcgt tgtctgcgat accattccgc tgagcgatga aatcaaatca ctgccgaacg
    31201 tgcgtactct gaccctgtca ggtatgctgg ccgaagcgat tcgtcgtatc agcaacgaag
    31261 aatcgatctc tgccatgttc gaacactaat cgaacccggc tcaaagaccc gctgcggcgg
    31321 gtttttttgt ctgtaatatc catttgtatg acctatgcct ccttcacctg ccatttagtt
    31381 gacagatgat gcgctcacgg atgaaacatt attgtgaaca aaatattttc ctcacatgtg
    31441 atgcctttcc gcgctctgat cgacgtttgc tggaaagaaa aatatactgc cgcacggttt
    31501 acccgtgacc tgattgccgg gataaccgtc gggattattg ctatcccgct ggcgatggcg
    31561 ttggctattg gtagtggtgt ggccccccag tacggtttat ataccgcagc tgttgcgggg
    31621 attgtcattg ctctaacggg tgggtcacgc tttagcgttt ccggtccgac tgcggcattt
    31681 gtggtaattc tctatcccgt ttcgcaacag tttggactgg caggactgct ggttgcgacc
    31741 ttgttgtcgg gaatcttttt gattctgatg gggctggcac gctttggtcg tctgattgag
    31801 tatattccgg tttccgtcac cttaggtttc acctcgggta tcgggatcac catcggtacc
    31861 atgcagatta aagattttct cggtctgcaa atggcccatg tcccggaaca ttatctacaa
    31921 aaagtcggcg cattatttat ggcgctgccg accattaatg tgggtgatgc tgccattggc
    31981 attgtgacgc ttggcattct ggttttctgg ccgcgtctgg gcattcgttt acccggtcac
    32041 cttccggcct tgctggctgg ttgcgcggtg atggggattg ttaacctgct cggcggacat
    32101 gttgctacca tcggttcgca attccactac gtcctggccg atggttctca gggtaacggt
    32161 attccgcaac tgctgccgca actggtgctg ccgtgggatc tgcctaattc agaattcacg
    32221 ctaacctggg attctattcg caccctgctg cctgcggcat tctcaatggc aatgctcggg
    32281 gcgatcgaat ctctgctctg cgctgtggta ctggatggta tgaccgggac taagcacaag
    32341 gcgaatagtg aactggtcgg tcaggggctg gggaatatca tcgctccatt ctttggtggt
    32401 attaccgcta ctgctgccat cgcgcgttct gccgctaacg tccgtgccgg ggcaacttcc
    32461 cctatctcgg cggtgatcca ctctattctg gttattctcg ccctgctggt actggcacca
    32521 ctgctctcct ggctgccgct ttccgccatg gcagccctgc tgttgatggt ggcgtggaac
    32581 atgagtgaag cgcacaaagt ggtcgacttg ctgcgtcatg cgccgaaaga tgacatcatc
    32641 gtcatgctgc tgtgtatgtc actgaccgtg ctgtttgata tggttattgc catcagcgtg
    32701 gggatcgtgc tggcatcgct gctgtttatg cgtcgtatcg cacgtatgac tcgcctggca
    32761 ccggtagtcg tagatgttcc agacgatgtc ctggttctgc gcgttattgg cccgctgttt
    32821 tttgctgctg ctgaaggctt attcacggac ctggagtcac gtcttgaagg caaacggatt
    32881 gtgattctga agtgggatgc cgttccggta cttgatgctg gtggtcttga tgcgttccag
    32941 cgttttgtga agcgtctgcc cgaaggatgt gaactgcgcg tgtgcaacgt ggaattccag
    33001 ccactgcgaa ctatggctcg cgcaggcatt caaccgatcc cgggacgcct cgcgttcttc
    33061 ccgaatcgtc gcgcggcgat ggcggattta taagtacgag attgaccagt cagcgcaaac
    33121 tgactggtca gcaaactgca ttatttgtta gctatgacgg cgattatcgc tacggcggca
    33181 acgtttgtca tagtgacgaa cccaccagta acggtcgcac acctgttcat gcccgccaac
    33241 acgggcaccc gccagccaga gaatggcacc gacgaaaata cttagcactg caccatgtgc
    33301 gaaatactgg ggcatattaa actgtggtaa ctggttgagg attgaatacc ccacgccgac
    33361 caccattacc accagaccca accccatgag cacgttaccg agtatcgaag cgtttttgcg
    33421 tttcatatgt cacctccgga actttctggg ttgtaacagg gaatacccct cttccttatg
    33481 tgtaaagtat agacaacacc ctgtgggtta tgtgcgggcg taatcacaat tacaaccctt
    33541 atttcaacaa aactttacaa ataaacgcct gaacaccttg actttctaat agtccactca
    33601 ggcaattatt acgttatctg catttgatac gcagtttttt gtcaagcggg gccgcaacca
    33661 gtaaactacg cgccagttat gtacacactc aggacaaaaa aacgtgacga ttaaattgat
    33721 tgtcggcctg gcgaaccccg gtgctgaata cgccgcaacg cgacataatg ctggtgcctg
    33781 gttcgttgac ttactggcag agcgtttgcg cgctccgctg cgcgaagagg ctaaattctt
    33841 tggttatact tcgcgagtca cccttggaag cgaagatgtc cgcctgttag tcccgactac
    33901 atttatgaat ctcagcggca aagccgttgc ggcgatggcc agttttttcc gcattaatcc
    33961 ggacgaaatt ctggtggccc acgacgaact ggatctgcct cctggcgtcg ccaaatttaa
    34021 gttgggcggt ggccatggtg gtcacaatgg actgaaagac atcatcagta aattgggtaa
    34081 taaccctaac tttcaccgtt tacgcatcgg aatcggtcat ccgggcgata aaaataaagt
    34141 tgtcggtttt gtgttaggca aaccgcctgt tagtgaacag aagttaattg atgaagccat
    34201 cgacgaagcg gcgcgttgta ctgaaatgtg gtttacagat ggcttgacca aagcaacgaa
    34261 ccgattgcac gcctttaaag cgcaataagt cgttgtctgc ggcatttttg ccgagtgccg
    34321 tgtataatag gcaaagttat ttccatttct gcaatctgtt agcaataaca ggttgattat
    34381 taagatatta aggtgattta aatcatggga ttcaaatgcg gtatcgtcgg tttgcccaac
    34441 gtcgggaaat ctaccctgtt caacgcgctg accaaagccg gtattgaagc ggccaacttt
    34501 ccattctgca ccattgagcc gaacacaggc gtcgtaccaa tgcctgaccc tcgcctggat
    34561 caactggctg aaatcgtaaa accgcaacgt acgcttccca cgaccatgga atttgtcgat
    34621 atcgccggtc tggtaaaagg cgcatcgaaa ggcgaaggtc tgggtaacca gttcctgacc
    34681 aacatccgtg aaaccgaagc gatcggtcac gttgttcgct gctttgaaaa tgacaacatc
    34741 attcacgttt ccggcaaagt taacccggct gacgatattg aagttatcaa caccgaactg
    34801 gcgctggcag acctcgacac ctgcgaacgt gcgattcatc gcgtacagaa gaaagccaaa
    34861 ggtggcgata aagacgcgaa agctgagctg gcggtcctgg aaaaatgcct gccccagctg
    34921 gaaaacgcag gtatgctgcg cgcgctggat ttaagcgctg aagagaaagc ggctattcgt
    34981 tacctgagct tcctgacgct gaaaccaaca atgtacatag ccaacgtcaa cgaagatggt
    35041 tttgaaaaca acccatatct tgaccaggtg cgtgaaatcg cggcgaaaga aggttctgtt
    35101 gtggttccgg tttgtgctgc tgttgaagca gacattgccg aactggacga cgaagaacgt
    35161 gacgagttta tgcaggagct tgggctggaa gaaccgggcc tgaaccgtgt gatccgtgcc
    35221 ggttataagc tgctgaacct gcaaacttac ttcaccgctg gggtgaaaga agtgcgtgca
    35281 tggaccattc cggttggagc aaccgcgccg caggcagcgg gcaaaatcca tactgatttt
    35341 gaaaaaggct ttatccgtgc acaaaccatc tcgtttgaag atttcatcac ttacaaaggt
    35401 gaacaaggcg cgaaagaagc aggcaaaatg cgtgcagaag gtaaagatta catcgtgaaa
    35461 gatggcgatg tgatgaactt ccttttcaac gtctaattaa tttattgtct catgaggttt
    35521 cgctaaatct catgaagagc gcaaaagtat ataaaatcca cgcaattgcg tggatttttc
    35581 atttcaccga gtcctaatca ttcttgtaac atcctacact tttgtgctta ttgcctacag
    35641 tgaaccacga gaataattaa agacataagt gttcagatga ctgatatctc attagcagtg
    35701 atataacgtc agtttttgtt ccactacaca tcagccctct tcacagctga tcaatattga
    35761 tctcgctcca atatgctggc gagaaaagga cttacctgaa gggtaatatc ccgcagataa
    35821 agtccccgaa taacgtgatt acgtgcgatc ctggcaagat gaaaatgata accagtagca
    35881 caataataac gataggccag aaaatccatt gaacaaaaat gaatatcgat gttttttgct
    35941 ttttcttatt agtaatgcaa aaatggcgca tattgttttt gttatgcaga tacatcaagc
    36001 gaaaatccca taactaatta ggattattac aaaaaatctt caccaaattt tttaaccgct
    36061 gtttgttgta atatttttgc catattcata gtaacgaatt ggaataagta acgacaactt
    36121 agggaataac tcgatataaa ctcacctttg taaggggggg gttaaatata tattaattcc
    36181 tgtgttcctg attttgcccg gtaatagaga attattctca gagaagctca tcatgattgc
    36241 accatacaga tatcatgatg ataagaggat attattcatt gacttaaaca aaatgacaaa
    36301 atccatagac ccatagcctg ggtactatat aggagtggta tgggcatcaa acaacacaat
    36361 gggaatacca aagccgatcg tctcgctgaa ttaaaaatcc gttcgccctc aattcaactg
    36421 ataaaatttg gcgctattgg tttgagtgca attatctttt cccccctgct gatagctgct
    36481 gatacaggaa gtcaatatgg caccaatatt actattaatg atggtgacag aattacagga
    36541 gataccgccg atccatcagg aaacctctat ggtgtaatga ccccagcagg aaacacgcct
    36601 ggcaatatca acctgggtaa tgatgtcacc gtcaatgtca acgacgcctc tggatatgca
    36661 aaaggaatca ttattcaggg caaaaacagc tccctgacag ctaaccgact cacagtagat
    36721 gttgttggtc aaacctctgc catcggcatt aacttaattg gtgactatac ccatgctgac
    36781 ttaggcacag gcagcaccat taagagtaac gatgacggca tcattattgg gcatagctca
    36841 acactaacag ccactcaatt caccattgaa aactcgaacg gtataggcct aaccatcaat
    36901 gactatggca ccagtgtcga tcttggaagc ggaagtaaaa tcaagaccga tggaagtaca
    36961 ggtgtttata tcggtggtct caacggcaat aacgccaatg gtgctgcgcg ttttacggcg
    37021 acagacctga caatcgatgt tcagggctac agcgccatgg ggataaacgt acagaaaaac
    37081 tctgttgtcg atctcggaac aaacagtacc attaaaacca atggcgataa tgctcacggc
    37141 ctctggagct ttggccaggt tagcgcgaat gcactcactg ttgatgtaac tggagccgcg
    37201 gccaatggcg tcgaagttcg tggtggtaca accactatcg gtgcagatag ccatatttct
    37261 tccgcgcagg gcggtggcct cgtcaccagt agttcagacg cgacaatcaa tttttctggc
    37321 acggcagcgc aacgaaacag catcttttcc ggcggttctt atggtgcctc ggcccagacg
    37381 gcaacggctg ttatcaacat gcaaaatacc gatattacgg ttgatcgtaa tggcagtctg
    37441 gcgctgggtt tgtgggcgct cagcggcggt agaataaccg gagacagttt ggctatcacc
    37501 ggcgcggcag gagccagagg gatttatgcc atgaccaaca gccagatcga cctcacgagc
    37561 gatctggtca ttgatatgag tacacccgac cagatggcca tcgcaacgca acatgacgat
    37621 ggttatgccg ccagccgcat caacgcctcg ggtcgtatgc ttatcaacgg tagcgttctt
    37681 tccaaaggtg ggctaatcaa tctggatatg caccctgggt cggtttggac aggttcctcc
    37741 ctcagcgata atgtcaatgg cgggaaactg gacgttgcaa tgaataacag cgtctggaac
    37801 gtaacaagta attctaatct cgacacgctg gcgctgagcc attcaactgt cgattttgcc
    37861 agccacgggt caactgccgg cacatttacc acattaaacg tagagaacct gagcggtaac
    37921 agtaccttta ttatgcgtgc tgatgttgtt ggcgagggta atggcgttaa taatagaggg
    37981 gatttattga atatcagcgg gagtagtgct ggtaatcacg tattggctat ccgcaaccag
    38041 ggcagcgagg ccacaacggg aaatgaagtt ctgacagtgg taaaaaccac tgacggcgcg
    38101 gcctcgttca gcgcgtcttc tcaggttgag ttggggggat atctgtacga tgtgcgtaaa
    38161 aatggcacta actgggagct ttacgcttcc gggacagttc cggaaccgac tcctaatcct
    38221 gaacccacac cagctcccgc tcagcctccc atagtcaacc ccgatcctac gcctgaaccc
    38281 gctcccacgc ctaaacccac cacgaccgca gatgctggcg gcaattatct caatgtcggt
    38341 tacttattga actatgttga aaaccgtacg ctgatgcaac ggatgggtga cttgcgaaat
    38401 cagagtaaag acggtaatat ctggttgcgc agttatgggg gaagcctgga ctcctttgcc
    38461 agtggcaaac tgagcggctt tgacatgggt tacagcggta tccagtttgg tggggataaa
    38521 cgtctctctg atgtaatgcc gttgtatgtc ggtctgtata ttgactcaac acatgcatcg
    38581 ccggactata gcggaggcga cggtaccgca cgttcagact acatgggaat gtacgccagt
    38641 tacatggcac aaaacggttt ttacagcgat ctcgttataa aagcatcgcg ccagaaaaat
    38701 agtttccacg tactggacag tcagaacaac ggcgttaacg ccaacggcac tgcgaatgga
    38761 atgagcatct ccctggaagc cgggcagagg ttcaacctgt cccctactgg ttatgggttc
    38821 tatatagagc cgcaaaccca gcttacatac agccaccaga atgagatggc tatgaaggcg
    38881 agtaatggcc tcaatataca tctgaatcac tacgaatcgc tgctggggcg tgccagcatg
    38941 atactggggt atgacatcac cgcaggcaac agccagctga atgtctatgt gaagactggc
    39001 gctatccgcg agttttcagg ggataccgaa tatctgttga acgactcccg ggagaagtac
    39061 agtttcaaag gtaatggctg gaataacggc gtgggagtca gtgcacagta taacaaacag
    39121 cacacattct atctcgaagc ggattacacg cagggtaacc tctttgatca gaagcaagtc
    39181 aacggaggat atcgcttcag cttttaagtg cgggatagac aagcccttaa acgatgctga
    39241 aaattgttag cgtgcgccgg aatgaaatac agatcactct gatgtatcag aaatcgtttc
    39301 tgtctttcat acccggcgct taaactgccc tgcatcaatg ccatgttgct tcattttccg
    39361 ccacagcgta gtgcggccga tcccaagtaa agccgacatt tcctgaatgc gaccgcctgt
    39421 gacctgggct gcgttaataa ttgcctcttt ttcaacttcc gcaaatgaca gactggtgga
    39481 aaggcgggtg gcgctgacat catctgtcgc ctgctcggta aacagatgtt caggtaaatc
    39541 actgacgcga atgcgcccgt tatcactact cagagcaaga ttctcgatga cgctgtaaag
    39601 ttcaaaatcg ttgcctggcc atgcacaaga aactaggcga gcgagggcat cgtcatcaat
    39661 tttcagccgc gtagagaagc gtttttcaag actgcgtaat ttgttattca ccagcgccgg
    39721 aatgctgcca cgccgcatac gcagaggcgg gatggtaatt tcaaatgcat gcagcgcgta
    39781 atacagctgg cgactaaaac gattttgttc caccagcatt gcgaggtccg cggtcgttgt
    39841 tgcaatcact ttgacatcaa ttggtattaa acgccgcgca tccagtcgcg tgataacccc
    39901 ctgcttgata acctgcagca aagcagactg taactccacc gccagatatt caatcttttc
    39961 aagaaacagc gtgccgccgt gtgccagttc cagccgactc agacggccat tttcattgtc
    40021 cgtgcgatcg ccaccaataa attcttccgc cagcgcagca tcaccatata actcacaatt
    40081 gacggcgata taaggacctg cagcacgctc gctttcatta tgaattgcct gacttagcag
    40141 tgccttgccc acgccctctt ctccacaaag caggacagga aagctactgc gcgccgcctg
    40201 gcgaccaaaa tgaatcaagc ggcgggtttg cggatcgtcc tgtggcatat gagcgaaggt
    40261 atggctgact tttcctaatt gactggtcat taactgccgc atctgttcca caggatggag
    40321 caacaaaata aagctggttc cctgcgtttc tattatcggt ttaagggtta tcaccgcatc
    40381 aataaactgg tgctggcttt caaaggttgc ttctacgtgt ttgagcggat gtgccagttt
    40441 tattgcttgt tgcaatacgg cgggtaacgt taagagttca gtgattgccc gtccctgact
    40501 tgccgtcgcg tcaaggcgca agacccgcgc cgcctgggca ttaataaatt gcaaattacc
    40561 ctgctcgtcc cagctaatca cgccatcatc catactttct aacagggcat taagttgatt
    40621 taaatgacgg ttagtttcag cgagcaaact gtccgtcagc agtaaattac cgacctcgcg
    40681 ggcgattgcc aacgtcaacg gcaaatcagc tgcggtagtt tgctcaaccg gacacgccag
    40741 cgctattgtt cccgtcaatc ggcccttgct gtcaaacaac ggcgttgcac aaaaggccca
    40801 gttccagagt gcctgtttga aatgttgatc ggccatcgtt ttcacggcct gaccagagat
    40861 agccgctaac gatagcgcac aagtaccaat aattccctcg gcgcaatacg tgccgtcatt
    40921 gaatcccagt gcacttagct gctgcaaggt ttgcggatcg ccattacggc tgagaatgca
    40981 ggcggtttca tcgaggataa acagcgcaca ctctcgcggt gccatatatt cccaggcatc
    41041 ttccagcgca gcctgcccga gcgtcagcat cgctttctta cgccgataaa tagaagcaaa
    41101 tgtcacgccc tgggcctgat gtggtacgtt ccatgtctcc cgtttcatca gcttattgca
    41161 tcgctcccag gaggttgcaa ttaagggaga tatgccccga ccatcgttgt taaaagcgcc
    41221 actcatatcc cgcatccctt atgacaaaag gtgttccggc tcgcaatttt aagtattaat
    41281 acacgtaaaa gtttcaatat ggaacattca ttaaagcata tgtttcacta tgaaacataa
    41341 tttgggctaa ttttcttttc ccttgcctga tgcacaatgg attcatcggc agtcagtggt
    41401 cgccgtgtcg ttgaacatca tccatgccct accgtaattg ctggagcaaa ataatgaaaa
    41461 aattgatcaa tgatgtgcaa gacgtactgg acgaacaact ggcaggactg gcgaaagcgc
    41521 atccatcgct gacactgcat caggatccgg tgtatgtcac ccgagctgat gcccctgttg
    41581 caggaaaagt cgccctgctg tcgggtggcg gcagcggaca cgagccgatg cactgtggtt
    41641 atatcggtca ggggatgctt tcgggggcct gtccgggcga aattttcacc tcaccgacgc
    41701 ccgataaaat ctttgaatgc tccatgcaaa ttgatggcgg cgaaggtgta ctgttgatta
    41761 tcaaaaatta caccggcgat attcttaact ttgaaacagc gaccgagtta ctgcacgata
    41821 gcggcgtaaa agtgaccact gtggtcattg atgacgacgt tgcggtaaaa gacagtcttt
    41881 ataccgccgg gcgacgcggc gttgccaaca ccgtattaat tgaaaaactc gtaggcgcag
    41941 cggcggagcg tggcgactca ctggacgcct gtgcggaact ggggcgtaag ttgaataatc
    42001 aaggccactc aataggtatc gctctcggtg cctgtaccgt tcctgccgcg ggcaaacctt
    42061 cttttaccct tgcggataat gagatggagt ttggcgtagg cattcatggt gagccaggta
    42121 ttgaccgccg ccctttctct tcccttgatc aaaccgtcga tgcaatgttc gacaccctgc
    42181 tggaacatgg ctcataccat cgcactttgc gtttctggga ttatcaacaa ggcagctggc
    42241 aggaagaacc acaaaccaaa caaccgctcc agtctggcga tcgggtgatt gcactggtta
    42301 acaatcttgg cgcaactccg ctttctgagc tgtacggcgt ctataaccgc ctgaccacac
    42361 gttgccagca agcgggattg actatcgaac gtaatttaat tggcgcgtac tgcacctcac
    42421 tggatatgac cggtttctca atcaccttac tgaaagttga tgacgaaacg ctggctctct
    42481 gggacgcccc ggtccacacc ccggccctta actggggtaa ataaggagaa agcaatgtca
    42541 ctgagcagaa ctcaaattgt taactggctc actcgttgtg gcgatatttt caacaccgag
    42601 agcgagtatc ttaccgggct ggatcgcgag attggcgacg ctgaccacgg gctaaatatg
    42661 aaccgaggct ttagcaaagt ggtggaaaaa ctccctgcta tcgcagataa agatatcggt
    42721 ttcattctca aaaataccgg tatgacgctg ctttccagcg tcggtggtgc cagtggtccg
    42781 ctgttcggta ccttctttat ccgcgccgca caggcgaccc aggcacggca aagcctgaca
    42841 ctggaagagc tttatcagat gttccgcgat ggcgcggacg gcgtaatcag tcgcgggaaa
    42901 gccgaacctg gcgataaaac catgtgtgat gtgtgggtgc cggtggtgga atcgttacgt
    42961 cagtccagcg agcaaaatct ctctgttccg gtggcgctcg aagctgccag tagcatcgcc
    43021 gaatccgctg cacaaagtac gattacgatg caagcccgca aaggccgcgc cagttatctc
    43081 ggtgaacgca gtattggtca ccaggatccc ggcgcgacct cggtgatgtt tatgatgcaa
    43141 atgttggcgt tagccgcaaa agagtaagga attggtgatg gtaaacctgg tcatagtttc
    43201 acatagcagc cgactgggag aaggtgtcgg tgaattagcc cgtcagatgt taatgagtga
    43261 tagttgtaaa atcgccattg ccgcgggaat tgacgatcca caaaatccca ttggtaccga
    43321 tgccgtcaaa gtgatggagg ccatcgaatc tgttgctgat gccgaccatg tgctggtcat
    43381 gatggatatg ggtagcgcat tattgagtgc tgaaactgcg ctggaattgc tggctcccga
    43441 gatcgccgca aaagtacgtt tgtgtgctgc gccgttggtc gaaggtacac tggcagcaac
    43501 ggtcagcgcg gcctcggggg cggatatcga caaagttatc tttgacgcta tgcatgcgct
    43561 ggaagccaaa cgtgaacaac tgggtttacc gtcctccgac actgaaatct ctgacacatg
    43621 tcctccgtac gatgaagaag cccgttctct ggcggtggtc ataaaaaacc gtaacggcct
    43681 gcatgtacgt ccggcctccc ggctggttta taccttatcg acatttaatg ccgatatgtt
    43741 gctggaaaaa aacggcaaat gcgtcacacc agagagtatt aaccagattg cgttactaca
    43801 agttcgctat aacgatacgc tgcgcctgat tgcgaaaggg ccagaagctg aagaggcact
    43861 gatcgctttc cgtcacctgg ctgaagataa ctttggtgaa acggaggaag tcgctccacc
    43921 tactctgcgt cccgttctgc ctgtttcggg taaagccttt tattatcaac cagttttatg
    43981 tacggtacag gcaaaatcaa ccctgaccgc ggaagaagaa caagatcgat tacgccaggc
    44041 tattgacttc acgttattag atctgatgac gttaacagcg aaagcagaag ccagcgggct
    44101 tgacgatatt gccgcaatct tttctggtca ccatacactg ttaggtgatc cggaactgct
    44161 ggcggcggca agcgaactcc ttcagcatga acattgcacg gcagaatatg cctggcagca
    44221 agttcttaaa gaacttagcc agcaatacca gcaactggat gatgaatatc tacaagctcg
    44281 ctatattgat gtggacgatc ttctgcatcg caccctggtc cacctgaccc aaacgaaaga
    44341 agaactcccg cagtttaact cgccaactat tctactggcg gagaacattt atccttccac
    44401 agtactgcaa ctggatccgg cggttgtaaa aggtatctgc cttagcgccg gaagtccggt
    44461 atcccacagc gccctaatcg cccgtgaact ggggattggc tggatttgcc agcagggtga
    44521 gaaactgtat gcgatacaac cagaagaaac gctaacgctg gacgttaaaa cgcaacgttt
    44581 caaccgtcag ggttaagcca ccgttcggat ggcatcgttc tgatgtcatc cggcaattta
    44641 cccagcactg atgtttttta actacctgtt atatttttta ccaatttatg atgatttttc
    44701 ttcgctaatc gcgaaccaaa tgtcgctggc tggctaataa acagacgtta tttgatcact
    44761 ctatttcgcc gttatttatc cctttaattc ccttttctaa aatgcctgac cgttcgcaga
    44821 atgagatttc gatcatgcag ctagtgcgat cctgaactaa ggttttctga tacttgaata
    44881 ccgttttatt cggtttcgcc aaaggagaat gattgatgaa atcccccgca ccttctcgcc
    44941 cgcaaaaaat ggcgttaatt ccagcctgta tctttttgtg tttcgctgcg ctatcggtgc
    45001 aggcagaaga aacaccggta acaccacagc cgcctgatat tttattaggg ccgctgttta
    45061 atgatgtgca aaacgtcaaa ctttttccgg accaaaaaac ctttgccgat gccgtgccta
    45121 acagcgatcc gctgatgatc cttgctgatt atcggatgca gcaaaaccag agcggatttg
    45181 atctgcgcca tttcgttaac gtcaatttca ccctgccgaa agaaggcgag aaatatgttc
    45241 cgccagaggg gcagtcactg cgcgaacata ttgacggact ttggccggta ttaacgcgtt
    45301 ctaccgaaaa caccgaaaaa tgggattctc tgttaccgct gccggaacct tatgtcgtgc
    45361 cgggcggacg ttttcgcgag gtatattact gggacaatta cttcaccatg ttaggacttg
    45421 ccgaaagtgg tcactgggat aaaatcgcgg atatggtggc caattttgct catgaaataa
    45481 acacttacgg tcatattccc aacggcaacc gcagttacta tttaagccgc tcgcaaccgc
    45541 ccttctttgc cctgatggta gagttactgg cgcagcatga aggcgatgcc gcgttgaagc
    45601 aatacctgcc gcaaatgcaa aaagaatatg cttactggat ggacggtgtt gaaaacctgc
    45661 aagccggaca acaggaaaaa cgcgttgtaa aacttcagga tagtaccctt ctcaaccgct
    45721 actgggacga tcgcgatacg ccacgaccag agtcatgggt ggaagatatt gccaccgcca
    45781 aaagcaatcc gaatcgacct gccactgaaa tttaccgcga cctgcgctct gccgctgcgt
    45841 ctggctggga tttcagctcg cgctggatgg acaacccgca gcagttaaat accttacgca
    45901 ccaccagcat cgtaccggtc gatctgaaca gcctgatgtt taaaatggaa aaaatcctcg
    45961 cccgcgccag caaagctgca ggagataacg cgatggcaaa ccagtacgaa acgctggcga
    46021 atgcccgtca aaaagggatc gaaaaatacc tgtggaacga tcaacaaggc tggtatgccg
    46081 attacgacct gaaaagtcat aaagtgcgca atcagttaac cgcggccgcc ctgttcccgc
    46141 tgtacgtcaa tgcggcagcg aaagatcgcg ccaacaaaat ggcgacggcg acgaaaacac
    46201 atctgctgca acccggcggc ctgaacacca cgtcggtgaa aagtgggcaa caatgggatg
    46261 cgccaaatgg ctgggcaccg ttacagtggg tcgcgacaga aggattacaa aactacgggc
    46321 aaaaagaggt ggcgatggac attagctggc acttcctgac caatgttcag cacacctatg
    46381 accgggagaa aaagctggtg gaaaaatatg atgtcagcac caccggaacg gggggcggcg
    46441 gtggcgaata tccattacag gatggctttg gctggaccaa tggcgtgacg ctgaaaatgc
    46501 tggatttgat ctgcccgaaa gagcaaccgt gtgacaatgt tccggcgacg catccgaccg
    46561 ttaagtcagc aacgacgcaa ccctcaacca aagaggcaca acccacacct taaccagcgc
    46621 ttactccgac aacgtcattc ggcttctccg gatggcgttg tcggcagata acaccatatc
    46681 tatggttttt tctacacaga cggtttttca agtcctggcg ggaagtattg cgcaaatgtt
    46741 tgccatacgt ggttgccgaa gatagcgtca tttctacggg taagtgtttc actttcatca
    46801 cccggcgatt tacgccgacg ttggctatat aaagcatcaa ttgctaccga atattgttac
    46861 ggtgttcact catcaacgtt tttttgcttt ctggagttaa attaataatc acataatcca
    46921 gacactccag agaatttagc aaccaaaaaa agaattcaga ccattcctct tcgcgtaact
    46981 gaagcattgc cgaacgggta ttttggctta atctgccaga aggattgatc aattctaaat
    47041 gttcaataag cgcagccaaa accgcctcac tatccgcccc atttttaacg cctgactggc
    47101 tactgtctaa aatatttatt ttctgaatca caccaaaaac aacgaaacaa taattaaaaa
    47161 aggattctgt tgttgttacc gggaaccgac ttatttcgct cagaaagtcg ttcaaccagc
    47221 tccgcagtga taagattcgc tgatgataca gtgtatcctc actgcgtgct ggaggtgcca
    47281 gtataatatg ttgatcaata cagtatttat tgtgttcagt cgtttcctga tgcgtttggt
    47341 tctctgcgga ttgtcgctgg cgaaataaat ggcttgcggt aaatgccgtt acagagcagc
    47401 gcaacgcgtc aattccttcg gagtgcccga cttttggcga gagttccggt tttatgcgtc
    47461 caggtagcgg aaatttcacc cgaatgagaa atgcattaat aaaatg
//
LOCUS       DAJTRM010000021        47343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRM010000021 DAJTRM010000000
VERSION     DAJTRM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.021"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..470
                     /gene="sseA"
                     /locus_tag="OO322_001276"
     CDS             <1..470
                     /gene="sseA"
                     /locus_tag="OO322_001276"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5906224.1"
                     /translation="QRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHENTAQ
                     IIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGV
                     SYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1289..2065)
                     /gene="sseB"
                     /locus_tag="OO322_001277"
     CDS             complement(1289..2065)
                     /gene="sseB"
                     /locus_tag="OO322_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5906225.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2207..3490)
                     /gene="pepB"
                     /locus_tag="OO322_001278"
     CDS             complement(2207..3490)
                     /gene="pepB"
                     /locus_tag="OO322_001278"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5906226.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3549..3749)
                     /gene="iscX"
                     /locus_tag="OO322_001279"
     CDS             complement(3549..3749)
                     /gene="iscX"
                     /locus_tag="OO322_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5906227.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3761..4096)
                     /gene="fdx"
                     /locus_tag="OO322_001280"
     CDS             complement(3761..4096)
                     /gene="fdx"
                     /locus_tag="OO322_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5906228.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4098..5948)
                     /gene="hscA"
                     /locus_tag="OO322_001281"
     CDS             complement(4098..5948)
                     /gene="hscA"
                     /locus_tag="OO322_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5906229.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5965..6480)
                     /gene="hscB"
                     /locus_tag="OO322_001282"
     CDS             complement(5965..6480)
                     /gene="hscB"
                     /locus_tag="OO322_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5906230.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6576..6899)
                     /gene="iscA"
                     /locus_tag="OO322_001283"
     CDS             complement(6576..6899)
                     /gene="iscA"
                     /locus_tag="OO322_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5906231.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6916..7302)
                     /gene="iscU"
                     /locus_tag="OO322_001284"
     CDS             complement(6916..7302)
                     /gene="iscU"
                     /locus_tag="OO322_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5906232.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7330..8544)
                     /gene="iscS"
                     /locus_tag="OO322_001285"
     CDS             complement(7330..8544)
                     /gene="iscS"
                     /locus_tag="OO322_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5906233.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8656..9144)
                     /gene="iscR"
                     /locus_tag="OO322_001286"
     CDS             complement(8656..9144)
                     /gene="iscR"
                     /locus_tag="OO322_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5906234.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9414..10154)
                     /gene="trmJ"
                     /locus_tag="OO322_001287"
     CDS             complement(9414..10154)
                     /gene="trmJ"
                     /locus_tag="OO322_001287"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5906235.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10273..11076
                     /gene="suhB"
                     /locus_tag="OO322_001288"
     CDS             10273..11076
                     /gene="suhB"
                     /locus_tag="OO322_001288"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5906236.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11221..12075
                     /locus_tag="OO322_001289"
     CDS             11221..12075
                     /locus_tag="OO322_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5906237.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12266..13546
                     /gene="csiE"
                     /locus_tag="OO322_001290"
     CDS             12266..13546
                     /gene="csiE"
                     /locus_tag="OO322_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5906238.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13538..14677)
                     /locus_tag="OO322_001291"
     CDS             complement(13538..14677)
                     /locus_tag="OO322_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5906239.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14837..15727)
                     /gene="hcaR"
                     /locus_tag="OO322_001292"
     CDS             complement(14837..15727)
                     /gene="hcaR"
                     /locus_tag="OO322_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5906240.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15863..17224
                     /locus_tag="OO322_001293"
     CDS             15863..17224
                     /locus_tag="OO322_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5906241.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17221..17739
                     /locus_tag="OO322_001294"
     CDS             17221..17739
                     /locus_tag="OO322_001294"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5906242.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17739..18059
                     /locus_tag="OO322_001295"
     CDS             17739..18059
                     /locus_tag="OO322_001295"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5906243.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18056..18868
                     /gene="hcaB"
                     /locus_tag="OO322_001296"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18056..18868
                     /gene="hcaB"
                     /locus_tag="OO322_001296"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5906244.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18878..20100
                     /locus_tag="OO322_001297"
                     /pseudo
     CDS             18878..20100
                     /locus_tag="OO322_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20197..20619
                     /locus_tag="OO322_001298"
     CDS             20197..20619
                     /locus_tag="OO322_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5906245.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20667..21539)
                     /locus_tag="OO322_001299"
     CDS             complement(20667..21539)
                     /locus_tag="OO322_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5906246.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21551..22645)
                     /locus_tag="OO322_001300"
     CDS             complement(21551..22645)
                     /locus_tag="OO322_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5906247.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22678..23676)
                     /locus_tag="OO322_001301"
     CDS             complement(22678..23676)
                     /locus_tag="OO322_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5906248.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23701..25212)
                     /locus_tag="OO322_001302"
     CDS             complement(23701..25212)
                     /locus_tag="OO322_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5906249.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25235..26217)
                     /locus_tag="OO322_001303"
                     /pseudo
     CDS             complement(25235..26217)
                     /locus_tag="OO322_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26314..29415)
                     /locus_tag="OO322_001304"
     CDS             complement(26314..29415)
                     /locus_tag="OO322_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5906250.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29422..29595)
                     /locus_tag="OO322_001305"
     CDS             complement(29422..29595)
                     /locus_tag="OO322_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906251.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29713..30906
                     /locus_tag="OO322_001306"
     CDS             29713..30906
                     /locus_tag="OO322_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5906252.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31104..32357)
                     /gene="glyA"
                     /locus_tag="OO322_001307"
     CDS             complement(31104..32357)
                     /gene="glyA"
                     /locus_tag="OO322_001307"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5906253.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32684..33874
                     /gene="hmpA"
                     /locus_tag="OO322_001308"
     CDS             32684..33874
                     /gene="hmpA"
                     /locus_tag="OO322_001308"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5906254.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33919..34257)
                     /gene="glnB"
                     /locus_tag="OO322_001309"
     CDS             complement(33919..34257)
                     /gene="glnB"
                     /locus_tag="OO322_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5906255.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34318..35652)
                     /gene="glrR"
                     /locus_tag="OO322_001310"
     CDS             complement(34318..35652)
                     /gene="glrR"
                     /locus_tag="OO322_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5906256.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35642..36355)
                     /gene="qseG"
                     /locus_tag="OO322_001311"
     CDS             complement(35642..36355)
                     /gene="qseG"
                     /locus_tag="OO322_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5906257.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36520..37947)
                     /gene="qseE"
                     /locus_tag="OO322_001312"
     CDS             complement(36520..37947)
                     /gene="qseE"
                     /locus_tag="OO322_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5906258.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38505..42392)
                     /gene="purL"
                     /locus_tag="OO322_001313"
                     /gene_synonym="purI"
     CDS             complement(38505..42392)
                     /gene="purL"
                     /locus_tag="OO322_001313"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5906259.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42651..44207
                     /gene="mltF"
                     /locus_tag="OO322_001314"
     CDS             42651..44207
                     /gene="mltF"
                     /locus_tag="OO322_001314"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5906260.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44204..44740)
                     /gene="tadA"
                     /locus_tag="OO322_001315"
     CDS             complement(44204..44740)
                     /gene="tadA"
                     /locus_tag="OO322_001315"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5906261.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44765..45400)
                     /gene="yfhb"
                     /locus_tag="OO322_001316"
     CDS             complement(44765..45400)
                     /gene="yfhb"
                     /locus_tag="OO322_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5906262.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45609..46457
                     /locus_tag="OO322_001317"
     CDS             45609..46457
                     /locus_tag="OO322_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5906263.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46796..47343)
                     /locus_tag="OO322_001318"
                     /pseudo
     CDS             complement(46796..47343)
                     /locus_tag="OO322_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11288 a        12969 c        11907 g        11179 t
ORIGIN      
        1 ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa ggagagttta
       61 acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg gcaagccatg
      121 aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca gaagttgatg
      181 aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt ccgtggacgg
      241 aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc
      301 gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta acggcagccg
      361 tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac gacggcgcat
      421 ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa gtatattaca
      481 ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc gcggcctttc
      541 cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa caatactcac
      601 cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag cgtattatcg
      661 acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag tccgaattca
      721 gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa gatattactt
      781 aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag gcggttattt
      841 gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa tgcaagcgcg
      901 tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag agagttatta
      961 ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg
     1021 cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct tgctatcgcg
     1081 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
     1141 cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt aaataataac
     1201 cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc
     1261 cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta aagtcacgca
     1321 aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa
     1381 ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat
     1441 cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc
     1501 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
     1561 tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct
     1621 ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg
     1681 gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg
     1741 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
     1801 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct aacgaggtga
     1861 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcactat
     1921 cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta
     1981 gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt
     2041 caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcgcaaa
     2101 tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg aatttgcaga
     2161 atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca
     2221 gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt tcaaccggcg
     2281 ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa
     2341 agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg cttccggtat
     2401 tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc agcggcagac
     2461 gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca agcgcatcgt
     2521 caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgaggg
     2581 tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga ccatcggcaa
     2641 gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg ttgcgatagg
     2701 tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca cagcagagga
     2761 acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc ccggtaaccg
     2821 ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt ttgatgctgt
     2881 agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc ggtgcttctt
     2941 tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgcc
     3001 cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg gtgatccgat
     3061 aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt gccagttgcg
     3121 atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg atgatcatca
     3181 ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc actttacgcg
     3241 tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca tcccagcctt
     3301 caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc gcacgctgga
     3361 tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta ttaatgctgt
     3421 aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta atcttcatcg
     3481 cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc cccaggcagc
     3541 ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag gattttctcg
     3601 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
     3661 gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt
     3721 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
     3781 tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc
     3841 ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
     3901 agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
     3961 aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg tcgagaatgg
     4021 tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga tgaggcaaaa
     4081 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
     4141 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
     4201 tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc ggcagcattg
     4261 tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
     4321 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg ggcttttacg
     4381 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
     4441 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
     4501 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc
     4561 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
     4621 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
     4681 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
     4741 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
     4801 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
     4861 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
     4921 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
     4981 cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
     5041 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
     5101 gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag ttcacgctga
     5161 acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc cgccagcaga
     5221 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
     5281 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
     5341 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt cggttcgtta
     5401 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
     5461 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
     5521 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagt
     5581 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
     5641 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
     5701 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
     5761 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
     5821 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
     5881 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
     5941 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
     6001 actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg ccgcgtccca
     6061 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
     6121 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
     6181 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
     6241 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
     6301 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgccag
     6361 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
     6421 cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat
     6481 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
     6541 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
     6601 ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag gccttctttt
     6661 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
     6721 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
     6781 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
     6841 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
     6901 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
     6961 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
     7021 agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
     7081 ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa aacgcgcgtc
     7141 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc cacaggccgg
     7201 tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac ccacgttacg
     7261 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
     7321 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
     7381 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
     7441 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
     7501 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
     7561 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
     7621 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
     7681 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
     7741 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca ttccgacgat
     7801 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
     7861 cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac ctttcgggcc
     7921 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgattgg
     7981 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
     8041 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
     8101 aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga taataccgtt
     8161 acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac ggcaagtatc
     8221 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
     8281 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
     8341 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
     8401 ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg ttccgtccat
     8461 cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
     8521 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
     8581 atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact ccggcctgat
     8641 tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg
     8701 tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag
     8761 ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat cacgccacag
     8821 cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc
     8881 atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt
     8941 gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag
     9001 acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc
     9061 caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata
     9121 gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt
     9181 tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa
     9241 ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt
     9301 atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga cctacgattc
     9361 tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca ctgttattcg
     9421 gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag gatattcaat
     9481 tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc
     9541 ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa
     9601 cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccattt
     9661 tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac cgccattgcc
     9721 aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg gcatttctgc
     9781 aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac cggggtattt
     9841 gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc
     9901 gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc
     9961 gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag ggcaatcgcc
    10021 tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc
    10081 atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga
    10141 atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg
    10201 aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag
    10261 tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt
    10321 aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt
    10381 aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt
    10441 aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat
    10501 caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat caaacgtctg
    10561 ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta
    10621 tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc acagctgaac
    10681 ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc
    10741 ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg caagctgttc
    10801 aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct
    10861 gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc
    10921 gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg tcataactat
    10981 atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat actggcgaac
    11041 atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt gatatcactc
    11101 acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt actttctttc
    11161 gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca aggaaaggtt
    11221 atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt tgttgttgcc
    11281 ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa
    11341 atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact
    11401 cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc catcgcaacc
    11461 atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg
    11521 ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa
    11581 ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa tgtggtgcgc
    11641 catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat tggcggggcg
    11701 aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt gatcctcgac
    11761 tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag tggctactta
    11821 cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat cccgctttta
    11881 ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc
    11941 ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt
    12001 tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg
    12061 aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga
    12121 catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac gcttgccaac
    12181 atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc tattcttatc
    12241 aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc
    12301 ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac
    12361 catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag
    12421 cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg gttatgacgg
    12481 tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg
    12541 acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag
    12601 tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt
    12661 agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata ttcatttcct
    12721 gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc ctcagtttaa
    12781 tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg
    12841 acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac tgtttatggc
    12901 attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc agcgggacag
    12961 acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg
    13021 tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc aggcgttaaa
    13081 tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta
    13141 tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt
    13201 ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct ggctaatgca
    13261 ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga
    13321 agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat
    13381 gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc
    13441 ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac
    13501 aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg
    13561 ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg ccgtggccca
    13621 gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga
    13681 cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca gcaatataac
    13741 gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc accactatca
    13801 accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg
    13861 agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc agcgcaaaga
    13921 taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc
    13981 cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata
    14041 aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac gcagaccaac
    14101 cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg atgagaaagc
    14161 cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga taatcaaaca
    14221 tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc
    14281 gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca
    14341 acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta
    14401 cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc agcacgcgca
    14461 aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa
    14521 aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc
    14581 ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag tatgtgaaat
    14641 agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt
    14701 tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt
    14761 cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg
    14821 caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga
    14881 aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag
    14941 gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta ttcatataac
    15001 cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg
    15061 ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg
    15121 gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg
    15181 cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc accactaatg
    15241 gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc
    15301 cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca
    15361 gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt ggcaataaat
    15421 tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc
    15481 gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac
    15541 attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga
    15601 cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt
    15661 ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta
    15721 gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt
    15781 cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg tcaatacatc
    15841 ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt accaactgat
    15901 tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca tttaccaact
    15961 ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa gccagatccc
    16021 aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg tggtgcgcca
    16081 gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag
    16141 ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct ggtcttatgg
    16201 cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg
    16261 taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag gactgatttt
    16321 tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca ttgcctggta
    16381 tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa
    16441 gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg accagtatca
    16501 tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga
    16561 taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct
    16621 gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa
    16681 tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg aagcagaaca
    16741 acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat tccccacgct
    16801 ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc ctgatcaagt
    16861 tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta aagccgcttt
    16921 tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga
    16981 gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca gcaaactgtg
    17041 tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg gcattactaa
    17101 ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag
    17161 tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa
    17221 atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct ctttcacgaa
    17281 gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga cgaagagatt
    17341 cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa aggcgtccag
    17401 ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg aatcgcccgt
    17461 ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca cttaatcagc
    17521 aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt gaattatcta
    17581 ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa
    17641 gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa
    17701 gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc
    17761 ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc gtcatcgcct
    17821 tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt
    17881 cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca
    17941 gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt
    18001 atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag
    18061 cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct
    18121 ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt cggcggcaaa
    18181 agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtgac
    18241 ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga
    18301 ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc
    18361 agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg gttacctgct
    18421 gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact
    18481 gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca gtaaacatgc
    18541 cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg
    18601 cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga
    18661 aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt
    18721 tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa
    18781 tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg
    18841 ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat
    18901 tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac
    18961 cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc
    19021 gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg
    19081 gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac
    19141 acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaac
    19201 cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct
    19261 acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt
    19321 gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagcgc
    19381 agcgcagcgc agcgcagatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc
    19441 cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccatcagca ggctggtgtg
    19501 cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg
    19561 ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt acggtattgg tatcagcgcc
    19621 aacgagcaac tggctcgcga ggccaacctt gatactgcca atggcattgt cattgatgag
    19681 gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt
    19741 gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt
    19801 gccgctgccg caatgttagg gctaccgcta ccgctactgc cgccgccgtg gttctggagc
    19861 gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt
    19921 cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc
    19981 ggtgcggtca cgctgaatca gggccgtgag attcgcccaa ttcgcaaatg gatccagagc
    20041 ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa atcactgtaa
    20101 tcaggataat tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt
    20161 acgtttaaat cacattatct tgcaaaggga ttggttatga acacactacg ttattttgat
    20221 tttggagctg cccgccccgt tttgttatta attgcccgta tcgccgtggt cttaattttc
    20281 attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg
    20341 ttgggcgcgc caatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc
    20401 gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt tattttctac
    20461 acgctgggta cggcggtgat tggtcaccat tactgggata tgaccggcga tgcggttggg
    20521 ccaaatatga ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt gctattggca
    20581 attaccggac cgggggcaat ttctctcgat cggcgttagg aaagatgccg gatgcggcgt
    20641 gaacgcctta tccggcattt tataaattac agccactcta cccgcaacga catctctgag
    20701 gttgttgatt cccctggtgc tagcgcaatg agatcacccc cttccgggcg atgatgctca
    20761 tccggcgcat ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac
    20821 gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg
    20881 gcataacctt cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg
    20941 ttcacccact gacgcggcaa cggcgcgagc tggttaaaat ccagttcctg cggtagctgc
    21001 tcgcaaaact cacccgccag ccactgctcc cgttccagcc agtaaccgct cgcctgcgcc
    21061 tgaatccgcg tttgtggtga caacggaaaa taaggatgcc agccggtacc aaatggcagc
    21121 gtctctgccc cttgattggt gacagagagc gtcaccgtca gcgtatccgc cgttaaatga
    21181 aacgcctgac ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa
    21241 ctatcatcgc tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag
    21301 tgcgcatccc actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacgg
    21361 ttgccgctca cccggtttgc aaatggcacc agcggaaaac atgaggcatc ggttgccaca
    21421 ccgctttttt taccaggacg taagagcggc gtcgtatcgc gccagaagcc ttcgattacg
    21481 ccgccctggt cagaaacgtc cagctttagc gacccgtggg ataaggtata gatggtcatc
    21541 cgctaactcc ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca
    21601 gcacataggc atcacctgcc tgttctagcg agaagcgatg ggtaatggcg ttacgcggcc
    21661 acagcttcca gtcagtcaaa tcgtgggcgc atttttccat atggaacaga ctggtcaccc
    21721 aggagccgat aatccgccgt tgatggtgca tcagatcagc gctgacctcg aattccactt
    21781 ttccggtttc accaatgtaa accacccgcc cccagtcagc agtggattgc agtgccagca
    21841 agcgacctgc ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtaagtt
    21901 cagcaattat ctgcggcaga ccttcggtgg ttgctaaata gccgtgatcc atccccccta
    21961 actgttttgc catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac
    22021 cgcgaccttt cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca
    22081 cgttatcgct gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc
    22141 aactgataaa cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt
    22201 cttccgccag cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc
    22261 ctttgccagt acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag
    22321 aaatgtgata caccagcacg cggtcgccct ctttaaaatg gcggcagcct tgccccatcg
    22381 ccacaatctg cccgcacggt tcatgaccgt tgataaatcc ctggtataac ggtttatcgg
    22441 gtgccgccgc cgtggcacgg tgttgatgat agatatagtg gacatcgctt ccgcaaatcc
    22501 cggaggattt cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc
    22561 gcagatcgac ggttgaattt cctagtaaat aagcagccag catcgttttc ataaatccct
    22621 cattaacaat acgattaatt ttcatccctg cagcacccgc gccaggggcg ttaaggttta
    22681 gcgtttcgct ttactgcttc gctgggtcag caagatattc gccagcaccg ccaccacgat
    22741 gatgacgccg cgtaccacct gctggaaaaa ggagttaata ccgagcagca ccagaccgtt
    22801 accgattagc gtaatcacca gcacaccaag caatgtaccg aacagggaac cgcgaccgcc
    22861 ggaaagtgcc gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc
    22921 ggcacctgcg ttaccggaac cgaggcgtgc cgccaacaaa atgccggtca ccgccgctaa
    22981 taatcccgaa agggtaaaga taagaatgcg cactcgacga acgttaatac cgcacaactg
    23041 cgccgccgtg gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg tttttcggct
    23101 aatgaacaca aacagcgcaa acaacaccat catgatcagc gcggatactg gcacaccgag
    23161 aaattgtccg cccagccagt ccagcacctc gttttcgtca atcggcactg gcagtgcgtt
    23221 ggtcataaac agccccattc cgcgcagagc gctccacagc cccagtgtgg caacgaaact
    23281 tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg cgcctaacag
    23341 caacaccagc agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa atgccaggca
    23401 caccgagaca aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat
    23461 cagcgtcatc gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg
    23521 gttattcaat gagataaagc caggcgcgtt cagggagaag accagataaa gaatggcaat
    23581 caccaccagc aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct
    23641 cttgccctgc ggcaatggta atgacgaagc atacatcttc aggttcctcg gtaaaattca
    23701 tcagtgcaca gacagaatgg cggacatcag ctcatccaca ttgaccggcg agtgaaactc
    23761 ctgcgagaac gtgccgtgct gtaataacag gatgcggtca cacaccagcg gcaattcctc
    23821 cacttcactg gagataaaca ccacgctttt tccttcggca gccagctcac ggacaatacg
    23881 gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat
    23941 ctggctggcg gcatagaccc aacgaccgat caccactttt tgctgattgc caccagaaag
    24001 cgtgccgatg ggtgtttcgc tactggcagc cttgaccgtc atccgctgca tcacctcttc
    24061 ggtcaggcgg cggatggtgg accattgcag cacaccgttg gtgctgattt tttgccgatt
    24121 ggtcagcact gtattttcgt caacgcctag ccagggaatg atccccgctt ctttgcggtt
    24181 ttctggcgta tagccaatgc cgcgtttcag catgtcgccg taatcggggc gcatgatttt
    24241 ctcgccgttg ataacaattt cgccctgttc atacgtctcc agcccaacaa tcgccttcag
    24301 caattcactg cgccctgccc ccagcaggcc agcaatgcca agcacttcgc cacgacgtag
    24361 cgtaaagctg atatcctcca gcttgggctt atggcgtaac gcacggactt ccagtacggc
    24421 ctgatccaca atttcctgag gggcaaccgg cgcaatatca acgtgatcac gccctagcat
    24481 tagcgacaca atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc
    24541 atcgcgcata acggtggcat aggaggcaat gcggcggatt tcttccatcc ggtggctgac
    24601 ataaatcacc gccacgccca gtgccgacat ctttttcacc gcactgatca ccagttcaac
    24661 ttctgcactg gcaagcgagc tggtgggttc atcaagaatg accacgcgcg gctcgccctt
    24721 catcacccgt gcaatttcca ccagttgctt ttgcgccggg cttagcgttg aaacaagttg
    24781 ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct gcgccatttg
    24841 caggtaatca atcatgccgt tgcggcgagg ccattgacca aggcagaggt tttctgccac
    24901 cgtcagccct tccaccagac ttaattcctg ataaaccgca cgaaccccca gttcagcggc
    24961 acggcgagtc aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc agatatcacc
    25021 gctatccggg cgttcgctgc cggtaagcat tcgaatgaga gtcgatttgc ccgcgccgtt
    25081 tttacctaac agcgcacgaa cttcgccttt attgagcgtg aagttaacgt tatccagcgc
    25141 aacgacgccg gggtaacgct tatttcctgc caccactttt gctaccggga ctgcctctgt
    25201 tgccgtgaac atagggacct ctgcgaatca gcgattaggg cagaccatca acgtgcgttg
    25261 ccagccactg tttaccatct tccgttttgg tatagagatc gattggcacc tgaatcactt
    25321 tttcaccgtc ggcttgttta ttgataacct tcaatgtttg cgcgaaaaca gcattgccca
    25381 ttttcttacc ggaaatatcc actaccgctt tcagcacctg attgttttcc agctcctgag
    25441 caatttcggt tgtcatatcc gaaccgaaaa cagcaatttt tccggcctga ttttgattac
    25501 gtaccgcttt taccgcgccg agtgtcgcac cgcccgactc ccccataatg gcgttgagat
    25561 ccggcgtgga gataatcagt ttttcaccaa cggaaatcgc tttatctaaa acagtccctt
    25621 cctggttagc gacaatttgc gcgccgggaa cgcgggattt taatacttct tcaaatcctt
    25681 tacgtcgctg cacacaaact tcaaaggctt cgcaattgat gacggcaatt ttcggctggt
    25741 caattttatt ggcaataaaa taatcggcgg cagcgttacc cagtttttac caaattccag
    25801 tggatcgccg accagatacg ccgagacata tttatcgaca cccttttgat taatacaggt
    25861 gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac ggctactgcc
    25921 attttcagac actgccgata aaataatggc atcgacatta cgcgccacga gggtatcaac
    25981 aaaggtactt tctttcgaaa tatccccctg ggcgttagtt tcaattaact gtacctgtac
    26041 tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat atccctgggt
    26101 atcaaggtat attgcaccaa tggtcatttc tttatccgca gccctggcaa atagtgcgct
    26161 acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt taggcatagt
    26221 gcctcctgta gggtttttat taacaacggc ttattctaat tattttgtga tgagcggcag
    26281 cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acgtagttaa aaataaaagg
    26341 catcacagtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc
    26401 gcgagcagac tcatattgcg caacctcccc gagtcccgcg gacgccagca tcgccaccat
    26461 caggcatttt tctttatgct gctgttgtaa atcgccatac agcgataaca ggtcaggttg
    26521 tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg cccactgttt
    26581 catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca gcgccattcc
    26641 ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt taatggtgcg
    26701 atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc cctgcgcgtt
    26761 ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta aacggccttc
    26821 gcttaaattc tccggataat acagcgcggc atgaagcagt tcgctggcct gctgcggctc
    26881 tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact gactggtgac
    26941 cttcccttcc ccgccttccc acgggtggaa tttacgcgtg gcgagaatgt ccgccgcttt
    27001 gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt
    27061 cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg tggctccact
    27121 taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata
    27181 agcattatca agataacgcg cggccagctc ataatcgtgt ttcttattcc acgcatggat
    27241 cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac gttgccataa
    27301 ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag caggccagta aatggcgagc
    27361 aaaccaacac tcttcaatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg
    27421 gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccacgtt cggctttcgg
    27481 cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat
    27541 ctctgccgcc agggtgggca ttccccagtt aatcagttgc ccggcggtca tcagagcgtt
    27601 aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc tacgaccatc
    27661 gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca gtttctcgcg
    27721 ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat gcaagcaaag
    27781 cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca gacccgcgtc
    27841 gaagttatca ttacgcgccg ccagtcgtgc cagaccataa tagccgcctg ctttactatt
    27901 gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc cctgacgttc
    27961 gtaagcactg gcgcgaatca aacttgcttg tccacactgc ggttttttgt tcagcgcatg
    28021 tgcacgtttc agcgcctgac tggcatacgc caccgcttgc gggaaatctg cgcggttata
    28081 ttccagcatc gccagcgcca ggttacagcg ataatccagc gggtccagcg ccacgccgcg
    28141 caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat gctgaccgat
    28201 aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc
    28261 cggcaacggc aactcctgcg gctgatgttc ctgataactc agtacaatat tgccgtcggc
    28321 atcggagagt tcaatggtca gtctgtcagg attgataccg tgcaacacgc cctggatggc
    28381 ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc cgatttcgcg
    28441 gatcgccaga cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg
    28501 ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcccagaga
    28561 atgataaggc aggaaatact gctcgaaacg cttctcttcg taagcatcaa gccaggtaaa
    28621 atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc cgttattgtc
    28681 ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccaactcc actgtttttt
    28741 acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat cgtgacacca
    28801 cgcgccgaca aaatcgtact gcgatttttc ggccatatat gaggttggaa caggcacatt
    28861 tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag tgccggtggc
    28921 gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgcagtca catccggtgg
    28981 gaaaacgctc tgatgccctt cacccccttt cactgccggg ttgacccacc acaagaaatg
    29041 acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagtgccg cccggtcagg
    29101 gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt cgccgaccca
    29161 caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca taaaggtggt
    29221 cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg gtcccagcat
    29281 ccccaccagc gcaggtttaa tgacttcatt gtgatagaca aaatcgcgct gtttcacttt
    29341 atcccatgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt tgatgtagtc
    29401 gttttccagc cacaccgcct gctaggattt cagggttttc tgctcgctca gcgtatcggt
    29461 cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat
    29521 gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct cttgccacac
    29581 ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg
    29641 gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa
    29701 ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca taacaaatgc
    29761 gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg
    29821 gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag cgaaaaacgg
    29881 gtggtgaata ttgacgatga aagccagacg cgcgggcata gtagcggtac atggctgatt
    29941 gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat tgagtgtcag
    30001 gttgctaatg cttgtttaag tccgaaaggc gaatttgagt atttacagat tgatgtaccg
    30061 acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg
    30121 ccggaccgca ccatcaatct ggcgctggca atccacgggc aggttgatcc ggtgaccggc
    30181 gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag ttgaggtgaa gtatctgctg
    30241 gaagagaagc tgggcattcg ggtgctggtc gataatgact gcgtgatgct ggcgctggcg
    30301 gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt tgattacggc
    30361 attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggtagcgga
    30421 cagattggtc acaccatcgt taatccggat ggagtcgtct gcgactgtgg acgttacggc
    30481 agcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt atggctaaaa
    30541 tcacaaccgg ttaatactca gcttgagcct gaaaaactga caaccgcgca gttaatcgct
    30601 gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc caatgccatt
    30661 ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt
    30721 cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac aggatttaac
    30781 ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt cgggcaatta
    30841 agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca gttacgacag
    30901 atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttgatga tcataaacga
    30961 aaaattgcct gatgcgctac gcttatcagg cctacacgga gattgcaata tattgaattt
    31021 gcaagatttt gtaggcctga gaaggtgttc atgccgcatc caacatgaac aacgagcaca
    31081 ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga
    31141 actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag cacgtcacac
    31201 atccagccag ccagttcttt cgcttcggct tctttaaagc cgcgacgggt aatcgccgga
    31261 gtacctacac gaataccgga ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt
    31321 ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc ggtcaggttt
    31381 ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac cactttgtag
    31441 ccgcgctcga ggaacacttc taccatcgct ttagcgtttt tcgcgacctg ctgctggtaa
    31501 gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc gattacgtgc
    31561 atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt gtacagctct
    31621 tcgctaccac ctttcgccaa gatcaggccg ccgcgcggac ccgccagggt tttgtgagtg
    31681 gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc agcaaccagg
    31741 cccgcaacgt gcgccatatc aacgaacaga taagcaccga tgctgtcagc gatttcacgc
    31801 attttcgccc agtcaaccac gccggaatat gcagagaaac caccgataat cattttcggc
    31861 ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga tatgaccggt agcatcgata
    31921 ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc gtgagtcagg
    31981 tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag cagcgcggtg
    32041 tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta gtcagcgccg
    32101 aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata ctcgcaaccg
    32161 ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg agaaccctgc
    32221 gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc
    32281 tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc ggcaatgttc
    32341 atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt ttggccttta
    32401 taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg ataacaggtc
    32461 ttgacaaagg aatttacgca aacgattacc ttcaggctac gcaaggcttt ggagaataaa
    32521 gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt tcacttcatc
    32581 acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt tttataagat
    32641 gcatttgaga tacatcaatt aagatgcaaa aaaggaagac catatgcttg acgctcaaac
    32701 catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa agttaaccgc
    32761 ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt ttaacatgag
    32821 taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg cctacgccag
    32881 taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac
    32941 cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt tggcaacgct
    33001 ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag cctatggtgt
    33061 actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc
    33121 cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc gcagcgcgct
    33181 tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat accgtccggg
    33241 gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa ttcgtcagta
    33301 ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc gcggtgaaac gcgaagaggg
    33361 tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt
    33421 cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga cgttaatctc
    33481 taccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa aagcaggcca
    33541 cacagcacaa gtgaactggt tccatgcggc agaaaatggc gaagttcacg cctttgctga
    33601 tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct ggtatcgtca
    33661 gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga tggatttgag
    33721 caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg gcccggttgg
    33781 cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg aaaacattca
    33841 ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat ggaaacatcc
    33901 ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat
    33961 gacccgagcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg tttgcgccgt
    34021 gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt
    34081 cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg tatggccttt
    34141 ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga cttcggccag
    34201 tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct ttttcatgct
    34261 attccttgaa aaggttgcct gtcttttgat ctgctaaacg taacacataa cgccaattca
    34321 ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa attctgtccg
    34381 gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt gcagcagctt
    34441 acgcaagtag ttgagttcaa aatgattacg tgcctcaaca aaggttggca gcgccgtatt
    34501 ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg aggtcagcgc
    34561 cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc tcgcggtcat
    34621 caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc gctctgccgc
    34681 ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg ccagtgccgg
    34741 aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt ccccgcgcgc
    34801 catcgctttt gacagatcac ggtgagtggc agaaataatc cgcacattga tatcaatatc
    34861 gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca gtttgacctg
    34921 taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt ccgccgcctg
    34981 gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag
    35041 ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa atggtttgct
    35101 gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg
    35161 accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg
    35221 cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc
    35281 ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga caggcttggt
    35341 gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag aaccatgcgc
    35401 agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa atagctgcat
    35461 accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt cgcgattcag
    35521 tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt cgctggtcag
    35581 gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata aatgcgcagg
    35641 tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca ctctcgtgcg
    35701 gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg
    35761 tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct
    35821 ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa cgttggcgct
    35881 cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa
    35941 cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg cggcgctcat
    36001 acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc
    36061 cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga tcagcacaat
    36121 ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc agcgcccaga
    36181 tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt ttttcttctg
    36241 ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat gccagacatg
    36301 gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt cgcatattca
    36361 ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga catggacagc
    36421 gcggagaaac cggggcgtac atatcgtacg tgaagatttc gagcactacc cggggccgaa
    36481 ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt ttttcgacga
    36541 cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat acagttcccc
    36601 ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc ccttcaccgc
    36661 cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg
    36721 cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat gtaaactgct
    36781 gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt aaagattatc
    36841 cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga gatcgacgtc
    36901 ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg tctccaccag
    36961 cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac ggttgtaatc
    37021 aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg ctatcaagaa tgctcaccac
    37081 ctctttttgc tctggcgtaa gcggcccgac aacctgatca gccagtaatt cagtgccctc
    37141 gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa attgatggcg
    37201 ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc caaccgagcg
    37261 taactcgctc ggtccacaga acgaaacgct attgcccaga gaacgccctt cccccagacg
    37321 gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga aaagtagcac
    37381 catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt gcccacgttc
    37441 ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg tggcctgcac
    37501 catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag catcgggacc
    37561 actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg cctggtaaag
    37621 tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg
    37681 gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac ggtagctacg
    37741 ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt
    37801 gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg cttgccatgc
    37861 cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt gtcgtaatga
    37921 gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga atagcctgat
    37981 gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt tgttatggaa
    38041 taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc tgatatcaga
    38101 agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg
    38161 ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact gtgccaataa
    38221 agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat ggcttcttcg
    38281 ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag ataaacttat
    38341 tagattgcaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt aaaccaccca
    38401 ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga tagtggttgg
    38461 tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc caactgctta
    38521 cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc cggatgccag
    38581 gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt gactcgacca
    38641 ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt
    38701 tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt gctttccagt
    38761 gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga gacagcaatc
    38821 ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt tacttcaacc
    38881 aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg ccacaactca
    38941 ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca tacccccagg
    39001 gccagcgttt gcggacgatg gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac
    39061 aggatagact tcgcccaacc ttcaccggca cccagcacat caccgtagga gaaacctccg
    39121 cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc cagtcagcag gtcgctcata
    39181 tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg ccgccatttc aacatgcgag
    39241 ttcacgccct gctcacgcag tacggcaact ttcggacgtg cgccagtggc aatatacggt
    39301 gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg atcggcgtcg
    39361 ttagatttcg cctgatgctc ctgatcggca cactccgggt tgtcacgcag gcgctgcatc
    39421 tgccaggtag tttctgccca ccagacacgc aacgtagtgc ggctttcgct gaatacagtc
    39481 tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgcccaac ataatggacg
    39541 caatccgcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc agcggcacga
    39601 acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc gtcacccaga
    39661 gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg ccatttccgc cagcgttacc
    39721 agcaggcctc catcagagcg gtcgtgatac gccagcagct tacgctgtgc aaccagcgcc
    39781 tgaatcgcgt catagaagcc tttcagttgg acaacatcgc gtacatctgc cggtttgtcg
    39841 ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc
    39901 aaatcaatca gcagcagtgc gttatcttcg gtacaaagct gcggcgtgat ggtgtgacgt
    39961 acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt catttcgcgc
    40021 tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc caccgggatc
    40081 gtcaggccca gcgccggaca aagttcttca cccacggctt taacggcttc atacaggccc
    40141 gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt aatgcgtttg
    40201 atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc cagacgggca
    40261 gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg
    40321 tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac ctgccacggc
    40381 cccaccatct gatcgcgcgc caccatgccg gttacgctgc ggtcaccgat ggtcaccagg
    40441 aaagttttct ccgccacggt cggcagatgc agtacgcgtt tcaccgcgtc tgcaatggtg
    40501 atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc gcgggtcatc
    40561 ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt atcaaaatga
    40621 cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg
    40681 cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac cgccagcacg
    40741 tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg ttcgtcgctt
    40801 agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt cgctcaccag ctccggcatc
    40861 gcgttagaaa gaccgcccgc accgacgtcg tggataaaca gaattgggtt agcatcacca
    40921 agctgccagc aacggtcgat cacttcctgg caacgacgct ccatctccgg gttgtcgcgc
    40981 tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga agacgccgcg
    41041 ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt cgcaccgacg
    41101 ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg
    41161 atcggtttgt gataaccgcg cagctcttcg ccattgtggc tgttcacttt ttcttcataa
    41221 gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc cgcaccgccc
    41281 agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc gctcaggctt accgaaatct
    41341 tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg atacggagaa accaaccaaa
    41401 ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc accgccggaa
    41461 ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc
    41521 atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc ccgtttcgtg gtcagcaaag
    41581 tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc agagagaacg
    41641 tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt cggctgttgt
    41701 tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc agagttcgcc
    41761 tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt gaaagcatcc
    41821 tgcagatagt caatttcatc ttccgccaga gccaagccaa gacgcaggtt agcgtcgatc
    41881 agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg
    41941 gcaaacaact gctctgcatc atctaaatca aaaaagaccg tttccatcat gcggtcgtgc
    42001 agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc tatatagtaa
    42061 gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc aatatcggtc
    42121 gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag gagtttgcct
    42181 tgcggggcgt ggctggcgag tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc
    42241 tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc gtaaatattg
    42301 tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac
    42361 agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa gctttcggtg
    42421 tacccaaggg gggggaaacg ggcgtcatta taaagaatct gatgcgctga cgaaaccgtt
    42481 tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg gctgtaataa
    42541 gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa cctttcacta
    42601 ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa ttgaaaaaat
    42661 taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc gctctctggc
    42721 catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa gcgcggggag
    42781 agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac gggaaacctt
    42841 ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta aaactgaaag
    42901 tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt aacgccgacc
    42961 tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag cctggcccta
    43021 cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc ccacgtacgc
    43081 tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggta gttaacgatc
    43141 tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac gacaaaaaag
    43201 gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc attgctgatt
    43261 ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg ctcgatatca
    43321 ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc ctttccgccg
    43381 ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc attgaagaga
    43441 aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt ttacgcgccg
    43501 tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa gaaattgact
    43561 ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag gccacttcac
    43621 cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc ctcggcatta
    43681 cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg caggatatga
    43741 tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc ctcgctgcgt
    43801 acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa accaaaggga
    43861 atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag aaaccctatt
    43921 acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat gtcgaaaata
    43981 ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag caggctacag
    44041 aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag ttgggcaaag
    44101 agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg acccattctc
    44161 cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc gtcgaggatt
    44221 gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca ctgagcaacg
    44281 ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc atacccggat
    44341 gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc gcaccaaaga
    44401 ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat ggttcaagcg
    44461 tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca ccctgccgca
    44521 gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg ttccagcctt
    44581 cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc cgctcatccc
    44641 aggcacgttt cgccagcgtc agcgcgtgac gcatccaata ttcgtggcta aattcgactt
    44701 cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga cgctatgctt
    44761 tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga tgctgacaga
    44821 aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc cgcagcggcg
    44881 tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc atcgtcaata
    44941 cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc ggcagccagg
    45001 gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc aaccagatat
    45061 cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga aaggcggtaa
    45121 cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt gcttcgctgt
    45181 gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca cgacctttta
    45241 tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca ttcagcggtt
    45301 ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc aatgttccat
    45361 ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc tctggactga
    45421 aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca cttttccggc
    45481 aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta gcggcaatgg
    45541 aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat ttcatcgcga
    45601 ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaagggctt gcccagagtg
    45661 ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat ttaagctctc
    45721 agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc gcgcaaaaac
    45781 tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg gcaagccagc
    45841 cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg ttacggctgg
    45901 tcggcgaaaa actgattaaa gaaaataccg ccgctatgta cgcaacgcta aacgttaata
    45961 gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg ataattctga
    46021 ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg atgaagattg
    46081 gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta caggcgtcgt
    46141 cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag ttaaacctgg
    46201 cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc tttactccga
    46261 atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa caagcgacaa
    46321 acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg ctgttcattg
    46381 cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt gaagcgctgg
    46441 tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt gtgttgtttt
    46501 gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga tatgtgtgaa
    46561 atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg aatgtgttgt
    46621 tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac accactttct
    46681 ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct gtgacataca
    46741 acattaactt ttcagtcaac cgtagcacgt tcttgtatac gccgtgtctg cggttttata
    46801 taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag atgatcgcca
    46861 ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat tattctggtg
    46921 aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc agtcctgctt
    46981 ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc acatggaaga
    47041 aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga aagttgatgc
    47101 ctcgttcgcg caacgcctcc accagcacga caagatgccg catactgcgc cccagccgat
    47161 ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cttgagcagt tttttcagcc
    47221 ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg catcctgcac
    47281 agttcaacgc attacgttgt agatcggtat tctggtcatt tgttgatacg cgtacatagc
    47341 caa
//
LOCUS       DAJTRM010000022        45521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRM010000022 DAJTRM010000000
VERSION     DAJTRM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.022"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            85..2238
                     /locus_tag="OO322_001319"
     CDS             85..2238
                     /locus_tag="OO322_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5906264.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2291..4021
                     /locus_tag="OO322_001320"
     CDS             2291..4021
                     /locus_tag="OO322_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906265.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4081..4572)
                     /gene="yajQ"
                     /locus_tag="OO322_001321"
     CDS             complement(4081..4572)
                     /gene="yajQ"
                     /locus_tag="OO322_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5906266.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4740..5651
                     /gene="panE"
                     /locus_tag="OO322_001322"
     CDS             4740..5651
                     /gene="panE"
                     /locus_tag="OO322_001322"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5906267.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5614..6204
                     /gene="yajL"
                     /locus_tag="OO322_001323"
     CDS             5614..6204
                     /gene="yajL"
                     /locus_tag="OO322_001323"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5906268.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6258..7706)
                     /gene="thiI"
                     /locus_tag="OO322_001324"
     CDS             complement(6258..7706)
                     /gene="thiI"
                     /locus_tag="OO322_001324"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5906269.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7912..8154
                     /gene="xseB"
                     /locus_tag="OO322_001325"
     CDS             7912..8154
                     /gene="xseB"
                     /locus_tag="OO322_001325"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5906270.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8154..9053
                     /gene="ispA"
                     /locus_tag="OO322_001326"
     CDS             8154..9053
                     /gene="ispA"
                     /locus_tag="OO322_001326"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5906271.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9078..10940
                     /gene="dxs"
                     /locus_tag="OO322_001327"
     CDS             9078..10940
                     /gene="dxs"
                     /locus_tag="OO322_001327"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5906272.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10995..11912
                     /gene="yajO"
                     /locus_tag="OO322_001328"
     CDS             10995..11912
                     /gene="yajO"
                     /locus_tag="OO322_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5906273.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12023..12541)
                     /gene="pgpA"
                     /locus_tag="OO322_001329"
     CDS             complement(12023..12541)
                     /gene="pgpA"
                     /locus_tag="OO322_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5906274.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12519..13496)
                     /gene="thiL"
                     /locus_tag="OO322_001330"
     CDS             complement(12519..13496)
                     /gene="thiL"
                     /locus_tag="OO322_001330"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5906275.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13574..13993)
                     /gene="nusB"
                     /locus_tag="OO322_001331"
     CDS             complement(13574..13993)
                     /gene="nusB"
                     /locus_tag="OO322_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5906276.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14013..14483)
                     /gene="ribE"
                     /locus_tag="OO322_001332"
     CDS             complement(14013..14483)
                     /gene="ribE"
                     /locus_tag="OO322_001332"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5906277.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTVLEMINVLKAIKA"
     gene            complement(14572..15675)
                     /gene="ribD"
                     /locus_tag="OO322_001333"
     CDS             complement(14572..15675)
                     /gene="ribD"
                     /locus_tag="OO322_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5906278.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15679..16128)
                     /gene="nrdR"
                     /locus_tag="OO322_001334"
     CDS             complement(15679..16128)
                     /gene="nrdR"
                     /locus_tag="OO322_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5906279.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16279..16818
                     /locus_tag="OO322_001335"
     CDS             16279..16818
                     /locus_tag="OO322_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5906280.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17116..18000
                     /gene="tsx"
                     /locus_tag="OO322_001336"
     CDS             17116..18000
                     /gene="tsx"
                     /locus_tag="OO322_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5906281.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18177..18524)
                     /locus_tag="OO322_001337"
     CDS             complement(18177..18524)
                     /locus_tag="OO322_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5906282.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18653..19624)
                     /gene="secF"
                     /locus_tag="OO322_001338"
     CDS             complement(18653..19624)
                     /gene="secF"
                     /locus_tag="OO322_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5906283.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19635..21482)
                     /gene="secD"
                     /locus_tag="OO322_001339"
     CDS             complement(19635..21482)
                     /gene="secD"
                     /locus_tag="OO322_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5906284.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21510..21842)
                     /gene="yajC"
                     /locus_tag="OO322_001340"
     CDS             complement(21510..21842)
                     /gene="yajC"
                     /locus_tag="OO322_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5906285.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21865..22992)
                     /gene="tgt"
                     /locus_tag="OO322_001341"
     CDS             complement(21865..22992)
                     /gene="tgt"
                     /locus_tag="OO322_001341"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5906286.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23047..24117)
                     /gene="queA"
                     /locus_tag="OO322_001342"
     CDS             complement(23047..24117)
                     /gene="queA"
                     /locus_tag="OO322_001342"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5906287.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24210..24783
                     /gene="acpH"
                     /locus_tag="OO322_001343"
                     /pseudo
     CDS             24210..24783
                     /gene="acpH"
                     /locus_tag="OO322_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24788..26605)
                     /gene="malZ"
                     /locus_tag="OO322_001344"
     CDS             complement(24788..26605)
                     /gene="malZ"
                     /locus_tag="OO322_001344"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5906288.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26761..28134)
                     /gene="proY"
                     /locus_tag="OO322_001345"
     CDS             complement(26761..28134)
                     /gene="proY"
                     /locus_tag="OO322_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5906289.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28210..29529)
                     /gene="brnQ"
                     /locus_tag="OO322_001346"
     CDS             complement(28210..29529)
                     /gene="brnQ"
                     /locus_tag="OO322_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5906290.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29936..31231)
                     /gene="phoR"
                     /locus_tag="OO322_001347"
     CDS             complement(29936..31231)
                     /gene="phoR"
                     /locus_tag="OO322_001347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5906291.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31289..31978)
                     /gene="phoB"
                     /locus_tag="OO322_001348"
     CDS             complement(31289..31978)
                     /gene="phoB"
                     /locus_tag="OO322_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5906292.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32168..33370
                     /gene="sbcD"
                     /locus_tag="OO322_001349"
     CDS             32168..33370
                     /gene="sbcD"
                     /locus_tag="OO322_001349"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5906293.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33367..36510
                     /gene="sbcC"
                     /locus_tag="OO322_001350"
     CDS             33367..36510
                     /gene="sbcC"
                     /locus_tag="OO322_001350"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5906294.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36636..37817
                     /gene="araJ"
                     /locus_tag="OO322_001351"
     CDS             36636..37817
                     /gene="araJ"
                     /locus_tag="OO322_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5906295.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37960..38868)
                     /gene="mak"
                     /locus_tag="OO322_001352"
     CDS             complement(37960..38868)
                     /gene="mak"
                     /locus_tag="OO322_001352"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5906296.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38993..39904
                     /gene="rdgC"
                     /locus_tag="OO322_001353"
     CDS             38993..39904
                     /gene="rdgC"
                     /locus_tag="OO322_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5906297.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39982..40437)
                     /locus_tag="OO322_001354"
     CDS             complement(39982..40437)
                     /locus_tag="OO322_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906298.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40434..40769)
                     /locus_tag="OO322_001355"
                     /pseudo
     CDS             complement(40434..40769)
                     /locus_tag="OO322_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40924..41208)
                     /gene="ppnP"
                     /locus_tag="OO322_001356"
     CDS             complement(40924..41208)
                     /gene="ppnP"
                     /locus_tag="OO322_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5906299.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41280..41957)
                     /locus_tag="OO322_001357"
     CDS             complement(41280..41957)
                     /locus_tag="OO322_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5906300.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42215..42406)
                     /gene="yaiA"
                     /locus_tag="OO322_001358"
     CDS             complement(42215..42406)
                     /gene="yaiA"
                     /locus_tag="OO322_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5906301.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42456..42980)
                     /gene="aroL"
                     /locus_tag="OO322_001359"
     CDS             complement(42456..42980)
                     /gene="aroL"
                     /locus_tag="OO322_001359"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5906302.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43163..43621)
                     /locus_tag="OO322_001360"
     CDS             complement(43163..43621)
                     /locus_tag="OO322_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5906303.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43741..44550
                     /gene="proC"
                     /locus_tag="OO322_001361"
     CDS             43741..44550
                     /gene="proC"
                     /locus_tag="OO322_001361"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5906304.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44567..>45521)
                     /gene="adrA"
                     /locus_tag="OO322_001362"
                     /gene_synonym="dgcC"
     CDS             complement(44567..>45521)
                     /gene="adrA"
                     /locus_tag="OO322_001362"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5906305.1"
                     /translation="PIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSRE
                     IYNLKTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLV
                     TLELTGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDG
                     MTGVYNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALT
                     RQLQITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLR
                     ISVGVAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11834 a        11881 c        11134 g        10672 t
ORIGIN      
        1 ccatataact ggacgattac attagaagta aactatactt tttatgaaat atcagggata
       61 aattattcac atggaaaata catcatgtac aaatcattat ttactattat cattctactg
      121 tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat aactttcatt
      181 gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat gaaactcgtt
      241 cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc aatttctgct
      301 cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa aaaattagaa
      361 actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga cggtaatggc
      421 actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc tgaatataca
      481 aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa acgtctggca
      541 atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg gaaagtcacc
      601 ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc atttgaagga
      661 tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa atcctggttt
      721 gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag agatggaaaa
      781 tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt ttctaatggt
      841 cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag taaacgtcat
      901 tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga tgggaaaatc
      961 aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga atattatcct
     1021 gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg aaacgatacc
     1081 agttactacc aatccggaaa aataagctct atacacaata gaaattcccg tggtcagtat
     1141 gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc cgtctataaa
     1201 gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga agaggagaaa
     1261 cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa aaatggtaat
     1321 cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga agagtggtac
     1381 gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga cggcattgcc
     1441 aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa tggcgtagag
     1501 aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga aaccctgtac
     1561 gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg taaactatta
     1621 acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta tgatcaaaac
     1681 ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt agacaatcca
     1741 acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc attagggaaa
     1801 tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa atctgcagag
     1861 caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa tggtatacct
     1921 gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg cagtagtaat
     1981 gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca gaatcttcct
     2041 gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc acatttctat
     2101 ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa agccaggttt
     2161 catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt aaatgaactt
     2221 gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac agaaaaggat
     2281 atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg ttttttattc
     2341 tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg tcgagaaaaa
     2401 actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg ctgaatggtt
     2461 taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca atttactgag
     2521 caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg cctccagagt
     2581 cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg ggttaaataa
     2641 accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa gcaaacaacc
     2701 cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt atttaccgac
     2761 tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata attctgtata
     2821 tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag ttgcggaggg
     2881 ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg cgctgaatat
     2941 gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg atgagtattt
     3001 gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg ttttatctca
     3061 acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc tggaaggaat
     3121 tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc tttcaaatta
     3181 catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg aattaagcct
     3241 cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc tggcgtttct
     3301 gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag acaaagtcag
     3361 cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat atttcacgac
     3421 tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa taagtaatac
     3481 tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg catctttcgc
     3541 tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga gaggactatt
     3601 ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa accgagtgtt
     3661 ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta taaatgccaa
     3721 agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta tgcataatct
     3781 gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac acattacctt
     3841 tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg tgggtaatga
     3901 gcaactttat cagattggca aaatctacca taagttggaa attaaacctg agccagagca
     3961 acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg caactcaata
     4021 aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat atccagaaag
     4081 ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc gcaccatcgc
     4141 catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat cgccctgaat
     4201 ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt tctgagtggc
     4261 gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat
     4321 attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag
     4381 aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga tggttttgct
     4441 ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc
     4501 gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc
     4561 gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt
     4621 taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca
     4681 tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa
     4741 tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta
     4801 aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt agtgtgaatc
     4861 tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc
     4921 tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca
     4981 aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac aacggcatgg
     5041 gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc accacccatg
     5101 ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg catatcggtc
     5161 cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa accgtgttgc
     5221 ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact
     5281 gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta cgtcatcatc
     5341 cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc gaagggcatc
     5401 atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcagaaa
     5461 atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca
     5521 atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc
     5581 tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc
     5641 gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt tcgcggcggt
     5701 atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac ctgctcgcgc
     5761 ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga atatgacgtg
     5821 atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac tctgctggtt
     5881 gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca
     5941 gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg cttcccgacg
     6001 ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg
     6061 gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct gaaaattatc
     6121 gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca
     6181 gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc
     6241 gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc ttgctcgcgc
     6301 agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag ccaggttttg
     6361 ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacatca
     6421 atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat atcgagaatc
     6481 acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg ctcggtctgc
     6541 tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg
     6601 aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt
     6661 ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt gccaatctgg
     6721 cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgtg
     6781 tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc
     6841 gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga tgcggcacgc
     6901 accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc
     6961 ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg
     7021 ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta
     7081 aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag
     7141 tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccacca
     7201 ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc cagatgaaca
     7261 gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg
     7321 ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt
     7381 acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt ctcaaaaata
     7441 tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata
     7501 cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg aacttcgatg
     7561 ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg
     7621 ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc
     7681 gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac
     7741 ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac
     7801 cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc
     7861 tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag
     7921 aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc
     7981 cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg
     8041 cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg
     8101 ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact
     8161 ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg
     8221 ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag
     8281 gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt ggcgttagca
     8341 caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac tcattaattc
     8401 atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg
     8461 tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct
     8521 cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt tcgatgattt
     8581 ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag
     8641 acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt cataaaaccg
     8701 gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc
     8761 gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg
     8821 atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag ggtgccgacc
     8881 agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag
     8941 cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa cagtcactcg
     9001 atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa
     9061 gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg
     9121 actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac
     9181 tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg
     9241 gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt gaccaattga
     9301 tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa
     9361 tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat
     9421 atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg
     9481 ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc gacggcgcga
     9541 ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt cctgatatgc
     9601 tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg cttaacaacc
     9661 atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag
     9721 ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa catattaaag
     9781 gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc ggcccggtgg
     9841 acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc
     9901 cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc
     9961 cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta
    10021 gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga
    10081 aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt
    10141 tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa cacgcagtga
    10201 cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc tactccacct
    10261 tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag ctaccggtcc
    10321 tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac cagggcgctt
    10381 ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg agcgatgaaa
    10441 acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg tccgcggtgc
    10501 gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctaccaattg
    10561 gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt ggtacgctga
    10621 tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat atgcgttttg
    10681 tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca
    10741 ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa gtgctgatgg
    10801 cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag
    10861 gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa
    10921 tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg
    10981 gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac
    11041 tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc
    11101 cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata aatttctttg
    11161 ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc gcactgcggg
    11221 atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc gttggtgatt
    11281 taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgacgtc
    11341 tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg
    11401 aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg
    11461 cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa cagcactgct
    11521 gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa gaagagcgcg
    11581 agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc
    11641 gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct gatgaggtgg
    11701 ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg
    11761 gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttaagta
    11821 aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt gatgagctgc
    11881 tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa acgccgtata
    11941 aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt
    12001 cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa
    12061 agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc
    12121 cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac
    12181 ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg
    12241 tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat
    12301 ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg
    12361 agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc gccagcgatc
    12421 ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc
    12481 acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac aaaatggtca
    12541 tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata
    12601 tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc
    12661 agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag
    12721 agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc
    12781 agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg
    12841 gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc
    12901 tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca
    12961 tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta
    13021 ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc
    13081 ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca
    13141 atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc
    13201 cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc
    13261 atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg
    13321 atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt
    13381 ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga
    13441 gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt
    13501 tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt ccgtgaatct
    13561 accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc
    13621 cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt
    13681 taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc gcaatgcgca
    13741 gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag ggcttcatca
    13801 gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga
    13861 cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt
    13921 tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag
    13981 cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta
    14041 atcatttcaa gcacggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca
    14101 cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg
    14161 ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg
    14221 gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta
    14281 gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg
    14341 ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg
    14401 tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca
    14461 acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg
    14521 gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc
    14581 cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg
    14641 ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt cgctgcctaa
    14701 tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta gcaatacgcc
    14761 agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac ccagttgcat
    14821 catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt
    14881 ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg
    14941 cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg
    15001 gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac
    15061 tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg
    15121 tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc
    15181 gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa
    15241 gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag
    15301 cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg
    15361 cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc
    15421 acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc
    15481 gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc
    15541 tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt
    15601 cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta
    15661 atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat
    15721 atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt
    15781 caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc
    15841 gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac tcaccggacg
    15901 tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac
    15961 gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg
    16021 ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg
    16081 agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga
    16141 ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc
    16201 ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg
    16261 acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg
    16321 ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc
    16381 acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac
    16441 aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca
    16501 agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca
    16561 gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc
    16621 agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat
    16681 gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt
    16741 ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt
    16801 caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatcttccc
    16861 cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg caacaagggc
    16921 gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa
    16981 tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc
    17041 tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca
    17101 aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct
    17161 tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg
    17221 caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac
    17281 gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat
    17341 gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt
    17401 tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac
    17461 cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt
    17521 cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga tatcgacact
    17581 ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc
    17641 gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt
    17701 accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc
    17761 gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct
    17821 atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct
    17881 cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc
    17941 aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga
    18001 ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt gtgctttgtt
    18061 catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat
    18121 tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg ttttaatcac
    18181 ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca
    18241 tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat
    18301 ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact gccatcttcc
    18361 gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttagaataa
    18421 acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga
    18481 tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag ttacctggat
    18541 gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc
    18601 aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt gattaaggca
    18661 gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct
    18721 tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca gtgccgatgg
    18781 aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca
    18841 gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg
    18901 tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac
    18961 gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg
    19021 atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga
    19081 taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac
    19141 ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa
    19201 ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg
    19261 tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg gtttcgcctt
    19321 cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct
    19381 cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga
    19441 gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc
    19501 ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc
    19561 agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg
    19621 ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt
    19681 acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta
    19741 gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc
    19801 agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt
    19861 gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg
    19921 ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt
    19981 gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca
    20041 ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc
    20101 agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag ggtcgggcca
    20161 attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt
    20221 gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga
    20281 cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca
    20341 tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg
    20401 atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta
    20461 acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccagtc
    20521 agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag
    20581 tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt taccagacgg
    20641 aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc ctgaataccg
    20701 ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca
    20761 agttggttta cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct
    20821 tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc
    20881 accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg
    20941 cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata cgggatgccc
    21001 ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt gccaagcgcg
    21061 gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc
    21121 atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc
    21181 acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca
    21241 gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt
    21301 ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg
    21361 cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc
    21421 agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac
    21481 acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg
    21541 acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca
    21601 atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact
    21661 tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt
    21721 ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac
    21781 atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc
    21841 attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg
    21901 acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa
    21961 acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa
    22021 tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta
    22081 gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat tgcggatttt
    22141 caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca
    22201 gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc
    22261 catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca gaatacggtg
    22321 catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat caaagccgat
    22381 atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc cctgaattat
    22441 accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac gcttcgccca
    22501 acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat acggcgtaca
    22561 ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca tcgacttttc
    22621 cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc cctgttcggt
    22681 gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg taagaatcgg
    22741 tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg
    22801 gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc
    22861 ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc
    22921 cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag
    22981 ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta aaccagcgcc
    23041 gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga taaacatcgc
    23101 atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg cattcatggt
    23161 gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg gcaagtggaa
    23221 gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa agatttgggt
    23281 atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga
    23341 acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac
    23401 cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt gatcttcaat
    23461 ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat
    23521 ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca gaccagcggt
    23581 cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt cgcggtcagc
    23641 gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc tgttgaggat
    23701 atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt
    23761 cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc
    23821 tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct caaccagcac
    23881 ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca cgcgggtatt
    23941 attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga aagtaccgtg
    24001 cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc gttcaggcat
    24061 gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac
    24121 actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc
    24181 tttacccccc tttggataga atataatcga tgaatttttt agctcacctg catttagccc
    24241 atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc ggaaatcctg
    24301 aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt attgactgac
    24361 aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg ccgcgttgcg
    24421 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    24481 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    24541 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    24601 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    24661 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    24721 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    24781 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    24841 tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac ggtgacgcgg
    24901 gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt tgctgattca
    24961 gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag ccgccacgac
    25021 gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg aataacgccg
    25081 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttaccat
    25141 ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    25201 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    25261 cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    25321 aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca taagagatgt
    25381 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcagcat
    25441 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    25501 cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc atattattgc
    25561 gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg tccatattcc
    25621 acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    25681 gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag tcgagcgccg
    25741 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg tgacaagctc
    25801 cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    25861 cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa cgcagcaacg
    25921 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    25981 cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc tttttcagat
    26041 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    26101 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    26161 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    26221 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca tcgacggcaa
    26281 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    26341 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    26401 tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta tgcattggta
    26461 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    26521 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    26581 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    26641 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    26701 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagta
    26761 ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc caatcaacag
    26821 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    26881 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    26941 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga aggcgatttg
    27001 cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg aagcgatcac
    27061 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    27121 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    27181 tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata cgtcactgtt
    27241 aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    27301 gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct gattccacgg
    27361 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    27421 ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    27481 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta ccatgccaag
    27541 ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg tcggttgccc
    27601 gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga tgatgatggt
    27661 ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca cgctcatcag
    27721 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    27781 gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    27841 ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt tttcctgcgc
    27901 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    27961 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    28021 tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac ccagtgccat
    28081 aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt ccatcaattc
    28141 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    28201 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    28261 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    28321 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    28381 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    28441 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    28501 caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca ccatcgagaa
    28561 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    28621 tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga ttaacgccgc
    28681 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    28741 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga acagcgccag
    28801 gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    28861 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    28921 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaatgcagc
    28981 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcgacaac
    29041 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    29101 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    29161 aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa aggaaacggt
    29221 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    29281 tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    29341 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    29401 agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa tgttacctgc
    29461 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    29521 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    29581 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    29641 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    29701 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    29761 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    29821 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    29881 aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag gcggattaat
    29941 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    30001 ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc acgatcgcta
    30061 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    30121 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    30181 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc ggcgtatgat
    30241 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    30301 ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    30361 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    30421 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    30481 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    30541 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    30601 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    30661 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    30721 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    30781 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    30841 gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg gtcagcacca
    30901 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    30961 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    31021 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    31081 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    31141 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    31201 ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag aaatcataag
    31261 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    31321 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    31381 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    31441 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    31501 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    31561 tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa atacggcgca
    31621 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg atatagtcat
    31681 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    31741 ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac tggataccgg
    31801 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    31861 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    31921 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    31981 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    32041 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    32101 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt ttcgccaggg
    32161 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    32221 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    32281 tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc cgcccagtta
    32341 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    32401 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    32461 gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    32521 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc gtgacattat
    32581 ttccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    32641 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    32701 catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg ccgtgcgtga
    32761 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    32821 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    32881 cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    32941 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taactcaacc
    33001 catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    33061 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    33121 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    33181 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    33241 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc tggatgaatc
    33301 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg ccggagaaca
    33361 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    33421 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat taccggccca
    33481 accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    33541 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgctgaa
    33601 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt ctggagccag
    33661 aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga gctggcgcgc
    33721 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    33781 ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt
    33841 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    33901 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcact
    33961 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    34021 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    34081 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    34141 gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    34201 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    34261 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    34321 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    34381 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    34441 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    34501 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    34561 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct ggcgcaacat
    34621 gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    34681 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    34741 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    34801 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc ccagcgtgca
    34861 cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    34921 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    34981 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    35041 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    35101 caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca ggacgatatt
    35161 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    35221 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    35281 attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact gacattgcca
    35341 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    35401 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac accgattctg
    35461 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    35521 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    35581 caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga caccgcgcta
    35641 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    35701 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    35761 ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    35821 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    35881 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    35941 cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca ggttgaggac
    36001 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    36061 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    36121 gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    36181 tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    36241 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    36301 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    36361 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgtagaagcg
    36421 atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct gggctacagc
    36481 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    36541 tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    36601 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    36661 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg ctcacggagc
    36721 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    36781 tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    36841 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    36901 cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat ggcgcatttt
    36961 ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    37021 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    37081 atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt aatattgcgg
    37141 tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa ggaaatctgc
    37201 gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc gccacgatgt
    37261 ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg atgtttattt
    37321 ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta gggatggtgc
    37381 tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc attgcagcag
    37441 tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc ggcatgaaaa
    37501 caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc
    37561 tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc gcaggtgggc
    37621 aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg atgctgacgc
    37681 tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt
    37741 catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg
    37801 cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg atgcggcgtg
    37861 aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa gacgcggcaa
    37921 gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg gccataacca
    37981 cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg gcgtttcaca
    38041 ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata aacggtctac
    38101 attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca cgacatgtgc
    38161 cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct
    38221 ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccactca aacgacgata
    38281 atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt taccgcaata
    38341 acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg
    38401 tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga atgccacgcc
    38461 cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc
    38521 atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca cttcccgctg
    38581 caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattgacatt
    38641 cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta ccgttccgcg
    38701 ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtctgccg
    38761 gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccag
    38821 tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg tactctcctt
    38881 atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg
    38941 acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg
    39001 gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat
    39061 ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat
    39121 gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca
    39181 aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga ttaaacaggc
    39241 gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga agaaaaccga
    39301 aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt tcagccgttt
    39361 tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg actgcgccag
    39421 tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt cgttaccggt
    39481 tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg ttcgctccgg
    39541 tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgctggaaga
    39601 tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca ccaatcacat
    39661 tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca ttcagtttgt
    39721 gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc gcgatcaaaa
    39781 cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca tcctgatgac
    39841 tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg
    39901 ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac
    39961 gaagccacac caggcatata attattcgct acggcgagca ataattttta gcgcagcaat
    40021 attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc tgtcagcagc
    40081 acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta acagctccgc
    40141 ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat ccgtcaggta
    40201 aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg caataaagtc
    40261 tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg gtaaatcggt
    40321 acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca ggttgatcgt
    40381 tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt gtgtcattgt
    40441 ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc ggttttcact
    40501 aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct
    40561 tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc cagcgaccga
    40621 tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc
    40681 gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat
    40741 ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact gatatttata
    40801 tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa aggaaatatt
    40861 attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg ggaaggcgag
    40921 gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat gaaactcact
    40981 gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg tcgcgtcagg
    41041 cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa
    41101 ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc tgctggaaaa
    41161 gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg
    41221 tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt cacgcaaaat
    41281 tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa gacaggaaca
    41341 tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca atccagcatg
    41401 atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt ttgttctgaa
    41461 tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg ccatttttgc
    41521 gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg atcttcaaca
    41581 atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt attacgcgca
    41641 gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc ctgattatcg
    41701 agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac atgggccagc
    41761 tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc gtactccgcc
    41821 atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt atcttcgtca
    41881 atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat ggtcaggatc
    41941 gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa ttaccgggca
    42001 agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca
    42061 gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg ttattgttta
    42121 atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc ccttgtagtg
    42181 gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg tcctcatagc
    42241 gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac gagtctggtt
    42301 taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt cctttttcaa
    42361 tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt
    42421 tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt ctgtgccagg
    42481 gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc
    42541 gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc
    42601 ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc
    42661 aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga
    42721 ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc cgcagttacc
    42781 gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc
    42841 tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg
    42901 ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc
    42961 agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg
    43021 gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt aaagaaaaaa
    43081 ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata ttcaagaatg
    43141 aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc caccacttct
    43201 ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt ccgccggtct
    43261 ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg
    43321 tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc tcgatggctt
    43381 cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac tgccggacaa
    43441 tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg
    43501 gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg
    43561 cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca
    43621 taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt
    43681 tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca
    43741 atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt
    43801 ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc ctccccggat
    43861 aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc gcaagaagtg
    43921 gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat taaagtgctt
    43981 agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc tgcaggtgtc
    44041 acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg
    44101 aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc
    44161 ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt
    44221 gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt
    44281 atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg
    44341 tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa
    44401 catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta
    44461 cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg
    44521 gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt
    44581 cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc
    44641 actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaag
    44701 ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc cgtaacatgg
    44761 cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca tcgccgccaa
    44821 accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa
    44881 tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa tggtcgatat
    44941 cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta
    45001 acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga gtactcatga
    45061 cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg acccagccaa
    45121 acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc ggagcactat
    45181 tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc accatcaaca
    45241 ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga cacagaatca
    45301 tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac attcccgcta
    45361 atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg gccctgctcg
    45421 ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc agccaccacc
    45481 agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg t
//
LOCUS       DAJTRM010000023        44176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRM010000023 DAJTRM010000000
VERSION     DAJTRM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.023"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            76..152
                     /locus_tag="OO322_001363"
     tRNA            76..152
                     /locus_tag="OO322_001363"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Met,seq:cat)
     gene            201..275
                     /locus_tag="OO322_001364"
     tRNA            201..275
                     /locus_tag="OO322_001364"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:233..235,aa:Gln,seq:ctg)
     gene            complement(429..1604)
                     /gene="ubiF"
                     /locus_tag="OO322_001365"
     CDS             complement(429..1604)
                     /gene="ubiF"
                     /locus_tag="OO322_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5906306.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1750..3174
                     /gene="miaB"
                     /locus_tag="OO322_001366"
     CDS             1750..3174
                     /gene="miaB"
                     /locus_tag="OO322_001366"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5906307.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3327..4367
                     /locus_tag="OO322_001367"
     CDS             3327..4367
                     /locus_tag="OO322_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5906308.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4364..4831
                     /gene="ybeY"
                     /locus_tag="OO322_001368"
     CDS             4364..4831
                     /gene="ybeY"
                     /locus_tag="OO322_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5906309.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4921..5799
                     /gene="corC"
                     /locus_tag="OO322_001369"
     CDS             4921..5799
                     /gene="corC"
                     /locus_tag="OO322_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5906310.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5824..7362
                     /gene="lnt"
                     /locus_tag="OO322_001370"
     CDS             5824..7362
                     /gene="lnt"
                     /locus_tag="OO322_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5906311.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7759..8667
                     /gene="gltI"
                     /locus_tag="OO322_001371"
     CDS             7759..8667
                     /gene="gltI"
                     /locus_tag="OO322_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5906312.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8837..9577
                     /gene="gltJ"
                     /locus_tag="OO322_001372"
     CDS             8837..9577
                     /gene="gltJ"
                     /locus_tag="OO322_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5906313.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9577..10251
                     /gene="gltK"
                     /locus_tag="OO322_001373"
     CDS             9577..10251
                     /gene="gltK"
                     /locus_tag="OO322_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5906314.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10251..10976
                     /gene="gltL"
                     /locus_tag="OO322_001374"
     CDS             10251..10976
                     /gene="gltL"
                     /locus_tag="OO322_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5906315.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11094..12029
                     /gene="rihA"
                     /locus_tag="OO322_001375"
     CDS             11094..12029
                     /gene="rihA"
                     /locus_tag="OO322_001375"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5906316.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            12113..13783
                     /gene="hscC"
                     /locus_tag="OO322_001376"
                     /pseudo
     CDS             12113..13783
                     /gene="hscC"
                     /locus_tag="OO322_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13843..15294)
                     /gene="djlC"
                     /locus_tag="OO322_001377"
     CDS             complement(13843..15294)
                     /gene="djlC"
                     /locus_tag="OO322_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5906317.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15291..15998)
                     /locus_tag="OO322_001378"
     CDS             complement(15291..15998)
                     /locus_tag="OO322_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5906318.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            16100..16654
                     /locus_tag="OO322_001379"
     CDS             16100..16654
                     /locus_tag="OO322_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5906319.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16664..18091)
                     /locus_tag="OO322_001380"
     CDS             complement(16664..18091)
                     /locus_tag="OO322_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5906320.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18088..18795)
                     /locus_tag="OO322_001381"
     CDS             complement(18088..18795)
                     /locus_tag="OO322_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5906321.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18959..19936
                     /locus_tag="OO322_001382"
     CDS             18959..19936
                     /locus_tag="OO322_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5906322.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(20006..20488)
                     /locus_tag="OO322_001383"
     CDS             complement(20006..20488)
                     /locus_tag="OO322_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5906323.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20723..23305
                     /gene="leuS"
                     /locus_tag="OO322_001384"
     CDS             20723..23305
                     /gene="leuS"
                     /locus_tag="OO322_001384"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5906324.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23320..23901
                     /gene="lptE"
                     /locus_tag="OO322_001385"
     CDS             23320..23901
                     /gene="lptE"
                     /locus_tag="OO322_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5906325.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23901..24932
                     /gene="holA"
                     /locus_tag="OO322_001386"
     CDS             23901..24932
                     /gene="holA"
                     /locus_tag="OO322_001386"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5906326.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24934..25575
                     /gene="nadD"
                     /locus_tag="OO322_001387"
     CDS             24934..25575
                     /gene="nadD"
                     /locus_tag="OO322_001387"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5906327.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25599..26210
                     /locus_tag="OO322_001388"
     CDS             25599..26210
                     /locus_tag="OO322_001388"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5906328.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26470..26787
                     /gene="rsfS"
                     /locus_tag="OO322_001389"
                     /gene_synonym="rsfA"
     CDS             26470..26787
                     /gene="rsfS"
                     /locus_tag="OO322_001389"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5906329.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26791..27258
                     /gene="rlmH"
                     /locus_tag="OO322_001390"
     CDS             26791..27258
                     /gene="rlmH"
                     /locus_tag="OO322_001390"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5906330.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27289..29190
                     /gene="mrdA"
                     /locus_tag="OO322_001391"
     CDS             27289..29190
                     /gene="mrdA"
                     /locus_tag="OO322_001391"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5906331.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29193..30305
                     /gene="mrdB"
                     /locus_tag="OO322_001392"
                     /gene_synonym="rodA"
     CDS             29193..30305
                     /gene="mrdB"
                     /locus_tag="OO322_001392"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5906332.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30316..31404
                     /gene="rlpA"
                     /locus_tag="OO322_001393"
     CDS             30316..31404
                     /gene="rlpA"
                     /locus_tag="OO322_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5906333.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31543..32754
                     /gene="dacA"
                     /locus_tag="OO322_001394"
     CDS             31543..32754
                     /gene="dacA"
                     /locus_tag="OO322_001394"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5906334.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32865..33128
                     /locus_tag="OO322_001395"
     CDS             32865..33128
                     /locus_tag="OO322_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5906335.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33229..33870
                     /gene="lipB"
                     /locus_tag="OO322_001396"
     CDS             33229..33870
                     /gene="lipB"
                     /locus_tag="OO322_001396"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5906336.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34129..35082
                     /locus_tag="OO322_001397"
     CDS             34129..35082
                     /locus_tag="OO322_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5906337.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35291..36256
                     /gene="lipA"
                     /locus_tag="OO322_001398"
     CDS             35291..36256
                     /gene="lipA"
                     /locus_tag="OO322_001398"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5906338.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36357..36560)
                     /gene="tatE"
                     /locus_tag="OO322_001399"
     CDS             complement(36357..36560)
                     /gene="tatE"
                     /locus_tag="OO322_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5906339.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36689..37477)
                     /locus_tag="OO322_001400"
     CDS             complement(36689..37477)
                     /locus_tag="OO322_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5906340.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37570..37953
                     /gene="crcB"
                     /locus_tag="OO322_001401"
     CDS             37570..37953
                     /gene="crcB"
                     /locus_tag="OO322_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5906341.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(38007..38216)
                     /gene="cspE"
                     /locus_tag="OO322_001402"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38007..38216)
                     /gene="cspE"
                     /locus_tag="OO322_001402"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5906342.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38391..38951)
                     /gene="pagP"
                     /locus_tag="OO322_001403"
     CDS             complement(38391..38951)
                     /gene="pagP"
                     /locus_tag="OO322_001403"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5906343.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39540..40925
                     /gene="dcuC"
                     /locus_tag="OO322_001404"
     CDS             39540..40925
                     /gene="dcuC"
                     /locus_tag="OO322_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5906344.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40966..41307)
                     /locus_tag="OO322_001405"
                     /pseudo
     CDS             complement(40966..41307)
                     /locus_tag="OO322_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41645..42154)
                     /locus_tag="OO322_001406"
     CDS             complement(41645..42154)
                     /locus_tag="OO322_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5906345.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42317..42562
                     /locus_tag="OO322_001407"
     CDS             42317..42562
                     /locus_tag="OO322_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5906346.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42537..44168
                     /locus_tag="OO322_001408"
                     /pseudo
     CDS             42537..44168
                     /locus_tag="OO322_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        11124 a        11043 c        11019 g        10990 t
ORIGIN      
        1 cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca
       61 tcgaagaaat aatctggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
      121 ggtcacaggt tcgaatcccg tcgtagccac caaattctga atgtgtcgaa tacgttcggc
      181 aaattcaaaa accaatttgt tggggtatcg ccaagcggta aggcaccgga ttctgattcc
      241 ggcattccga ggttcgaatc ctcgtacccc agccacatta aaaaagctcg cttcggcgag
      301 ctttttgctt ttctgcgttc attcaatgtc gaatgcgatg ttgacacgtc ttatccttca
      361 atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg ctggcacgca tccggcaatg
      421 ttgtaaggct acaaccctaa cgcatatttc agcgcctgac gtttcaacac gccagcacgc
      481 tctgccgcca ttaacccgag attacgcaca aaacgcagcg gcggcagatt attactgaat
      541 ccggcataaa acagatccat accactttgc ataatgaagt tatccgccat gcgccgcatc
      601 tggtaacgct tgaggacagg ataactggcc cacgcttcac cgtagctgcg ggcattcacc
      661 agaacatcaa tcagggcatc gacatcacga taaccaagat tcaccccctg ccccgccagc
      721 ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc ccggctgcac atactgcaac
      781 gcatgtcggc gcgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta acccagacgc
      841 gacgggaaat gcttcgcgat ttccgcctgg agttgcgcca tattcatatt ctgcaactgg
      901 cgaatacgcg tcggagagtc ataccacacc agcgatgccc agttatcaaa caacggcaga
      961 aacgcacgcg gtccgtccgg ggtaaattgc tgccaggtgc tgtcgccggg atcgttctca
     1021 cactgtacgc taatcaacat gcacgactgc gcatactgcc aggcatgaac gccaattccc
     1081 gccatctgcc gcacctgaga atttgcgccg tcggcaccaa tcaccagttt cgcgcggatc
     1141 gtttcaccgc ccttcagttc cagctcctga agatcattat ctcgatgtaa tgcaatcagc
     1201 gaggttggca cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca caacgcctgt
     1261 tgcagcactg tgttttccac catgtagcca agcaacggca actttaattc ggctgcgtca
     1321 aataccacat gtgccgtttc ccactcccag gtttccagtc tgcggtaagg atggcaacgc
     1381 atagcctgta ctgcatccca gacccctaac cctttaagca atgataccga agccgcgctg
     1441 atcgccgaga tccgcacgtc cggttggctg tcagcgacaa acggtgcagg ttctgcgtgc
     1501 tcgatcaccg ttaccgaaaa tccgtgctgt gccagcccca gcgccagtgc gccgccgacc
     1561 attcctccgc cgacaatggc aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt
     1621 agcaatatgc tttaagttta caggattttt acccccaagg gctgataacg ctcatactgg
     1681 tcacaacggc agcaaagcat tacactatgc gccctgcatt cctggctact atttcgcaag
     1741 agcaagtcga tgaccaaaaa actccatatt aaaacctggg gctgtcagat gaacgagtac
     1801 gattcatcga agatggccga tctgctggat gccacccacg gctatcaact gaccgacgtg
     1861 gcggaagaag cggatgtgct actgctgaac acctgctcaa tccgcgagaa ggctcaggaa
     1921 aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag agaagaatcc agacctgatt
     1981 atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac
     2041 tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc
     2101 gtgcgcggcg accgcagccc ggttgtagat atcagcttcc cggaaatcga gaagtttgac
     2161 cgtctgccgg aaccgcgcgc cgaagggccg accgcgtttg tctccatcat ggaaggctgc
     2221 aataaatatt gcacctactg cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg
     2281 tctgacgata ttctgtttga gattgcccag cttgcggctc agggcgtgcg tgaagtcaac
     2341 ctgctcggtc agaatgtgaa tgcctggcgt ggtgagaact acgacggcac caccggatcg
     2401 tttgccgatc tgttgcgtct ggttgctgcg atcgacggga tcgatcgtat tcgctttacc
     2461 accagccatc cgatcgaatt caccgacgat atcatcgaag tgtaccgcga cacgcctgag
     2521 ctggtgagct tcctgcatct gccggtacag agcggttccg atcgcattct gaacctgatg
     2581 ggacgtaccc atacggcgct ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt
     2641 ccggatattc agatcagttc tgacttcatt gttggcttcc ctggcgaaac caccgaagac
     2701 ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg acatgagcta cagctttatc
     2761 ttctctgcac gtccaggaac gcctgccgcc gatatggttg atgatgttcc ggaagaagag
     2821 aagaagcagc gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat ggcatggagc
     2881 cgtcggatgc tcggcaccac ccagcgtatt ctggtagaag ggacttcgcg taagagcatc
     2941 atggagcttt ccgggcgtac cgaaaataac cgcgtggtca acttcgaggg cacaccggat
     3001 atgatcggta aattcgtcga tgtagaaatt accgacgtct acccgaactc tctgcgcggt
     3061 aaagtggtgc gaactgaaga tgaaatgggt ctgcgcatgg cagaaacacc ggagtcggtg
     3121 attgcccgca cccgcaaaga aaacgacctt ggcgtgggtt attatcagcc gtaattctca
     3181 ggcctgccgt tccggtgggc cttgtttttt ctctctttat ccccatcttt atcgcaatgc
     3241 ttgcgccttt ttcctgtggc gtgaataatt tccttatagg ccggtgaatg ttcagcttcc
     3301 ggcggcatac agataagagg aacggtttga acatagacac tcgcgaaatc accctggagc
     3361 cagcagacaa tgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc
     3421 tcgaacgccg tctcggcatc gagatcaatc gccgcgataa ccactttaaa ctgaccggcc
     3481 gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc gatactgccc
     3541 cgatgcgcgg tcagattcag gatatcgaac cggaacagat ccaccttgcg attaaagaag
     3601 cgcgggtact ggagcaaagc gcggagagcg tgccggagta cggcaaagcg gtcaatatca
     3661 aaactaaacg cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac atcgccaata
     3721 ttctcgacca tgacattacc ttcggcgttg gcccggcggg tacgggtaaa acctacctgg
     3781 cagtggctgc ggcagttgat gccctggagc gtcaggaaat tcgccgtatt ctgctgactc
     3841 gtccggcggt cgaagccggt gagaaactgg gcttcctgcc tggcgattta agccagaaag
     3901 tagacccgta tctgcgccca ctgtacgacg cgctgtttga aatgctgggc tttgagaaag
     3961 tcgagaaact gattgagcgc aacgttattg aagtcgcgcc gctggcctat atgcgtggtc
     4021 gtacgctgaa cgacgcgttt atcattctcg atgagagcca gaacactacc atcgaacaga
     4081 tgaagatgtt cctgacccgt atcggtttta actcaaaagc ggttatcacc ggcgacgtca
     4141 cacagatcga cctgccgcgt aatactaaat caggcttacg tcatgccatt gaagtgctgg
     4201 ccgatgtcga agagatcagc tttaacttct tccacagcga agacgtggtt cgtcacccgg
     4261 tggtggcgcg tatcgttaac gcttatgaag cctgggaaga agcagaacaa aaacgtaaag
     4321 cggcgctggc agcagaacgc aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct
     4381 cgatttacaa ctggcatgtg aagataattc cgggttaccg gaagagagcc agtttcaaac
     4441 atggctgaat gcggtgatcc cgcagtttca ggaagagtcg gaagtgacta ttcgcgtggt
     4501 cgataccgcc gaaagccaca gtctgaatct gacctatcgc ggtaaggata agccgaccaa
     4561 cgtgctctcc ttcccgtttg aagtgccgcc tggcatggag atgtcgctac tgggcgatct
     4621 ggttatctgc cgtcaggtgg ttgagaagga agctcaggag caaggcaaac cactggaggc
     4681 gcactgggcg catatggtgg tgcacggcag tctgcatttg ttaggttacg atcacattga
     4741 agatgacgaa gcagaagaaa tggaagccct cgaaacagag attatgcttg ctctgggcta
     4801 tgaggatccg tacattgccg agaaagaata atttgccagg catttgactg gcagacgtcc
     4861 ccgccgttgg tgattaacga cggcgttttg attaactaac atgagaaccc atacgacgcc
     4921 atgagcgacg acaattcaca cagtagtgac acgataagca acaagaaggg atttttctcc
     4981 ctgttactca gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg
     5041 atccgtgatt ccgggcagaa cgaccttatc gacgaagata cgcgcgatat gctcgaaggg
     5101 gtgatggaca tcgcagacca acgcgttcgc gacatcatga tcccccgctc ccagatgatt
     5161 accctgaaac gcaaccagac gctggacgaa tgccttgatg tcatcatcga gtccgcccac
     5221 tcacgtttcc cggtgattag cgaagacaaa gatcacattg aagggattct gatggcgaaa
     5281 gacttgctgc cgtttatgcg cagcgatgct gaagccttca gcatggacaa agtgttacgt
     5341 caggcggttg tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct
     5401 cagcgttacc acatggcgat cgttattgac gaattcggtg gggtttccgg tctggtgacc
     5461 attgaagaca tcctggaact gattgttggt gagattgaag acgagtatga cgaagaagat
     5521 gatatcgact tccgtcagct gagtcgtcat acctggaccg tgcgcgcact ggcttccatt
     5581 gaagacttca acgaagcgtt cggcacccac tttagcgatg aagaagtcga cactatcggt
     5641 ggtctggtga tgcaggcgtt tgggcatctt ccggcgcgtg gcgaaactat cgacatcgac
     5701 ggttaccagt tcaaagtggc gatggccgat agtcggcgta ttattcaggt tcatgtcaaa
     5761 atcccggatg actcacccca gccaaagctg gatgaataaa accgaaactg gatagataac
     5821 tacatggatt ttgcctcatt aattgaacgc cagcgcattc gcctgctgct ggcgttatta
     5881 ttcggtgcct gcggaacgct ggccttctct ccttacgacg tctggcctgc ggcgattatt
     5941 tcgctgatgg ggcttcaggc gttgaccttt aaccgtcgcc cactccagtc tgccgctatt
     6001 ggcttttgct ggggatttgg cctctttggc agcggtatta actgggtcta tgtcagcatc
     6061 gcgacctttg gcgggatgcc tggcccggtt aacatcttcc tggtggtgct gctggcggcg
     6121 tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt cgcgtctgtg gccgaaaacc
     6181 acctggctgc gcgtggcgat tgccacccct gctctctggc aagtgaccga gtttctgcgc
     6241 ggttgggtgc tgactggctt cccgtggtta cagttcggct atagccagat tgatggcccg
     6301 ttaaaagggc tggcaccgct aatgggcgtg gaagccatta acttcctgct gatgatggtt
     6361 agtggcctgc tggcactggc gttggtcaaa cgcaactggc gtccgctggt ggtggccgtc
     6421 gtgctgtttg ccctaccctt cccgctgcgt tacatccaat ggtttacccc gcaaccggag
     6481 aaaaccattc aggtttcgat ggttcagggc gatattccgc aatcgctgaa atgggacgaa
     6541 ggccagcttc ttaatacgct aaagatttac tacaacgcaa cggcaccgct gatgggcaaa
     6601 tcatcgttga ttatctggcc ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg
     6661 ttcctcaaag cactggacgg tgagttgcgt gataaaggta gctcgctggt aaccgggatt
     6721 gtcgacgcgc gtctcaataa gcagaaccgc tacgatacct acaacaccat catcacgctg
     6781 ggtaaaggtg caccgtacag ctacgaatca gccgatcgct ataacaaaaa ccatctggtg
     6841 ccgtttggcg agtttgtccc gctggagtcg attctgcgtc cgttagcacc gttctttgat
     6901 ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc ggcaaatggt
     6961 attgagctta ctgcggctat ttgctacgag attattctcg ccgagcaagt gcgcgataac
     7021 ttccgcccgg ataccgacta cctgctgact atctccaacg atgcgtggtt tggtaaatct
     7081 attggtccat ggcaacactt ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca
     7141 ctgttgcgca gcaccaacaa cggcattacg gcggtgattg gcccgcaggg tgagattcag
     7201 gcgatgatcc cgcagttcgc ccgcgaggtg ttaaccacta acgtgacgcc gaccaccgga
     7261 ctcacaccat acgcacgtac cggcaactgg ccgctgtggg tgctgacggc actgtttggt
     7321 tttgctgctg tgttgatgag tctgcgtcag cgacgtaaat aatcccttct tatcgtgccg
     7381 gaatacatcc catatttcgg cacgctcacc ggatgcggca tcagtgcatt atccggataa
     7441 caatattctc ctatccaact ttggcacatc tattgctttg ttatacaagg caaagcttga
     7501 accagcatca gtacaacccg gttgcaccac agcaatccac cggtagcacc aaaagagtgc
     7561 attttaagaa ttttgcttca gaatggtgcg gtgcacctcg caaaaacaaa caataaaaca
     7621 tcaatatctt tacatttagc caaactaaat gttacctaac aggcacaaca ctgcacaata
     7681 aagttgcaga cgataacaac acaaacactc acaacgggta tccatgcgtt cttaacgcag
     7741 aagataaagg tgttggatat gcaattacgt aaacctgcca cagcaatcct cgccctggcg
     7801 ctttccgcag gactggcaca ggcagatgac gccgccccgg cagcaggcag tacgctggac
     7861 aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg aatcttcagt acctttctct
     7921 tattacgaca atcagcaaaa agtggtgggt tactcgcagg attactccaa cgccattgtt
     7981 gaagcagtga aaaagaaact caacaaaccg gacttgcagg taaaactgat tccgattacc
     8041 tcacaaaatc gtattccact gctgcaaaac ggcactttcg attttgaatg tggttctacc
     8101 accaacaacg tcgaacgcca aaaacaggcg gctttctctg acactatttt cgtggtcggt
     8161 acgcgcctgt tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa
     8221 gccgtagtcg tcacttccgg cactacctct gaagttttgc tcaacaaact gaatgaagag
     8281 caaaaaatga atatgcgcat catcagcgcc aaagatcacg gtgactcttt ccgcaccctg
     8341 gaaagcggtc gtgccgttgc ctttatgatg gatgacgctc tgctggccgg tgaacgtgcg
     8401 aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc cgcagtctca ggaagcctac
     8461 ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc tgatggatga cactatcgct
     8521 caggtgcaga cctccggtga agcggaaaaa tggtttgata aatggttcaa aaatccaatt
     8581 ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa
     8641 gaaccgaatg acaaggcact gaactaatta caagaaccag gggcggaaat tccagccctc
     8701 tcgattgtta cgtagcacgg acagactata cgcctgatgg tcgttcccca tcgggcctga
     8761 aaaccgcaat acgctgggta acaatcttcg agggtagcag ttaacgctgc taccctcttt
     8821 tttctggagt agatttatgt ctatagactg gaactggggt atttttttac aacaagcccc
     8881 gttcggcaat accacctatc ttggttgggt ctggagtggt tttcaggtga cgatcgcttt
     8941 gtcgatctgc gcctggatta tcgctttcct cgtcggttca tttttcggca ttttacgtac
     9001 cgttccaaac cgttttctct ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt
     9061 gccgttgatt gtgcaattct ttacctggta tctggtgatc ccggagctgc tgccggagaa
     9121 aatcggcatg tggtttaagg ccgagctgga tcccaatatt cagtttttcc tttcatccat
     9181 gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg ccgctattca
     9241 gtcgctaccg cgtgggcaga agaatgccgc gctggcgatg ggcctgacgc taccgcagac
     9301 ttatcgatac gtactgctgc ctaatgctta tcgcgttatc gtcccgccga tgacttccga
     9361 gatgatgaac ctggtgaaaa actccgccat cgcctcgacc atcggtctgg tggatatggc
     9421 ggcgcaggcg ggtaaattgc tcgactactc ggctcacgcc tgggagtcgt ttaccgccat
     9481 cacgcttgcg tatgtgttga ttaacgcttt tatcatgctg gtgatgacgc tggttgaacg
     9541 taaagttcgc ctgcccggca acatgggggg caaataatgt acgagtttga ctggagttcc
     9601 attgttcctt ccctgccata tctgctcgac gggctggtga tcaccctgaa aatcaccgtc
     9661 acggcagtcg tgattggtat tttgtggggt accatgctgg cggtgatgcg cttgtccagc
     9721 ttcgcgcctg tttcgtggtt tgccaaagcc tacgttaacg tattccgctc gattccttta
     9781 gtcatggttt tgctgtggtt ttacttgatt gtgccgggat ttctgcaaaa tgtgctggga
     9841 ttatcgccaa aaaatgatat tcgtttaatt tcggcgatgg tggcgttttc gatgtttgaa
     9901 gcggcctact attcagagat tatccgtgcc ggtattcaaa gtatttcccg tggtcagtcg
     9961 agcgccgcgc tggcgttggg gatgactcac tggcagtcga tgaaactgat tattctgccg
    10021 caggctttcc gcgcgatggt gccgctgctg ctcactcagg gcatcgtact gttccaggat
    10081 acctcgctgg tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc aaccattggt
    10141 gagcgtgatg gtacgcaggt cgagatgatc ctgtttgccg gatttgttta tttcgttatt
    10201 agccttagcg cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata atgattaccc
    10261 tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc tcaaccgaag
    10321 tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga
    10381 ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg
    10441 tggttaacga caagaaaacc gatctggcaa agctgcgctc ccgcgtcggg atggtattcc
    10501 agcatttcga gctgttccct catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga
    10561 aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc attgaaattg ctggagcgcg
    10621 tgggactttc cgcacatgcc aataaatttc cggcgcagct ttccggcggt cagcagcagc
    10681 gcgtggcaat cgctcgcgca ttgtgtatgg ctcctattgc gatgctgttc gacgaaccga
    10741 catcggcgct ggatccggag atgatcaacg aagtgctgga cgtgatggtg gaactggcga
    10801 acgaaggaat gaccatgatg gtggtgaccc acgaaatggg ctttgcccgc aaagtggcga
    10861 atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga ctcgccgaaa gacgctttct
    10921 tcgatgatcc gaaatcggac cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac
    10981 tctggcgcac atacccaatt gcgcgccatc tcacgcttta tcgtttcgcc cctcgtatct
    11041 gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca acaatggcac
    11101 tgccaattct gttagattgc gacccaggtc atgatgacgc tatcgcaata gttctcgcac
    11161 tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag
    11221 aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc gatattccgg
    11281 tggcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg
    11341 gcgaaagcgg tctcgacggc ccggcattac cggaaccgac gttcgcaccg caaaactgta
    11401 cggcggtgga gctgatggcg aaaacgctgc gcgaaagtgc ggaacctgtc acgattgttt
    11461 ctaccggacc gcaaactaac gttgccttgc tgctcaatag ccatccggaa ctgcatagca
    11521 aaattgcccg tatcgtgatt atgggcggcg caatggggct tggtaactgg acgcctgcgg
    11581 ctgaatttaa tatttacgtt gacccggaag cggcagaaat tgtcttccag tcagggatcc
    11641 cggtggtgat ggccggtctg gatgttactc ataaagcgca aatccacgtt gaagacaccg
    11701 agcgtttccg cgcgattggc aaccctgttt caaccattgt tgccgaactg ctggatttct
    11761 tcctcgaata tcataaagac gaaaaatggg gctttgtcgg cgcaccgctg catgacccat
    11821 gcaccatcgc ctggctgttg aaacccgagt tgtttaccac tgttgagcgc tgggttggcg
    11881 tggaaacaca ggggaaatat acccagggta tgacggttgt tgattattat tatctgactg
    11941 gcaataaacc gaatgccact gtaattgtcg atgttgatcg tcagggcttt gttgatttac
    12001 tggctgatcg tctgaaattt tacgcttaac actcgtcatt gattaagatc accacgcgtt
    12061 acctgggtgg tcttaatcta tttatctctt tttctttcaa gatataaatg caatggataa
    12121 tgcagaactc gccataggta tcgatctcgg cactaccaat agtttaattg ccgtctggaa
    12181 agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa tatttaacac catccataat
    12241 tagcatggat gaaaataatc atattttagt cggaaaaccg gctgtatcac ggcgtacttc
    12301 gcatccggat aaaacggcag cgttatttaa acgtgcaatg ggcagtaata ccaactggcg
    12361 gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac gctcattaaa
    12421 agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct ccgttctggc
    12481 ttatttcagc gatgaacaac gcaagcatac ccgtttagca gcggacttag ccgggttaaa
    12541 tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcgtatggcc tgcataccca
    12601 acaaaatacc cgttcgctgg tgtttgatct cggtggcggc acgtttgacg ttacggtgct
    12661 tgagtacgcc acgccggtga ttgaagttca cgcctccgct ggcgacaact tcctcggtgg
    12721 cgaagatttt acccatatgt tggtcgatga cgttttaaag cgcgcggatg tcacaaggac
    12781 cacgcttaac gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg caaaatgtag
    12841 caatcaatcg ccaatgcaca ttcgctggta gtaccaggat gaaacgcggg aatgcgaatt
    12901 ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc gagtgccgat
    12961 tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa atcgacagtc tggtgctggt
    13021 tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt
    13081 accgtatcaa agttacgatc cgagcaccat tgtcgcgctg ggcgcagcaa tccaggccgc
    13141 ctgccgctta cgcagtgaag atattgaaga agtgatcctc actgatattt gcccttactc
    13201 gttgggcgtt gaggttaacc gccagggcgt ttccggcatt ttctcgccga ttattgaacg
    13261 aaacaccact gtgcccgtgt cgcgtgtaga aacttattca accatgcacc cggaacagga
    13321 ttcaattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaaca acattctggt
    13381 ggaatccttc gatgtgccgt tgaagaaaac cggggcttat cagtcgattg atattcgctt
    13441 tagttatgat atcaacgggt tgcttgaagt tgacgtgctt ctggaagacg gcagcgttaa
    13501 gtccagagtg attaaccaca gcccggtaac attgagcgcg cagcagattg aagagagtcg
    13561 gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctcatcaatc gcacctttaa
    13621 agccaaactg gaagagttgt gggcgcgggc gctgggtgac gagcgagaag agattggccg
    13681 ggtgatcacc gattttgatg cggcgctgca gtcaaacgat atggcccgcg tcgatgaggt
    13741 caggcggcgg gcgagcgttt atttagccat tgagacctca taatcacggc tgccggatgc
    13801 ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat cgtcaactaa acaacatcgc
    13861 gattttgccg agagcataac ctataaccag caccgttaaa ctgattttga taatgctggt
    13921 aatgctctgt ggtttttcac cctcatcatt tttttcttca tggttttcag gtgttggctc
    13981 atcgacatct tcatcctccg gctcgagttt tactttcgtc aacagcatcc gggtgataat
    14041 ggcctctaat ggatgagcat ctccgccttc cagccttgta ttaagacggg ccagcatctc
    14101 ttgtttcgga aacgcttcaa gtactacctg ttccgctacc cgatacaaat ccagtaacgg
    14161 tgaatcttct tctgtctcgc taccgactct cgatttccac cacgcccatg ctttgcgttt
    14221 gaagaataac gactcaataa acgattgacc taacggcgat aattttccgc tatgtaaaat
    14281 ttccgcccat aacagccgcg tccgggcgct ctcaccagga acatatttca gcggttgacc
    14341 ttgcgcatct accatgctcc gcgcttcgat aaccatcact aataacgcga aataatccgg
    14401 gcaatgacgc ttgctccact gcaacaacaa attatcagct tgtgtagatg ggtaactttc
    14461 ccagtacgcg ccgagatcag caagcaggct ttccccctcg ccgcagtaca aacgttgtgc
    14521 acactggtaa taccgggcat cttcgctttc tggttcttcc gcttgccact gtttggctac
    14581 aggaatgagt tccaccatac ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg
    14641 taactttgca tcatcaggaa tgaaccacgg tccttccatc gccaaaaacg cagtgacata
    14701 gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tattcaacag tctggttctg
    14761 taacgcgacg ggatattcca gaaggctaag gaaatcgaaa caatcaccag cctgaatggc
    14821 ctcaagaaaa gcctcccttt cttcctcatc cattccttcg ctgtcattaa acgaatgcca
    14881 gttcaaacgc tctgccaaca aagaagcaca atttaatgac agatatcgcg cttctatagc
    14941 gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg tatttaattg
    15001 ctgaataaat ttctgccagg cggagggctg aaaacgatca ctttctgaat ccagtaatgt
    15061 cctgaatgca cgtagtattt catgttcagc ggtggcatct ttttcttcat catctgcttc
    15121 ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta atgctttaaa
    15181 cccctgcgga tcggtttcgg gatgacacaa cggtaagcga gcaagataag cctggcggat
    15241 aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt tcataggtcg
    15301 ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggctg ccagggaagc
    15361 gtggcataga aacggtcgct tcccgctgcc agctcttcga acatccgact cccggcgcta
    15421 tcgtatttgt acagggttag cgcctcgcgt agcgaagcgt tgtcttcgca ctgagaagat
    15481 tgccagtaga gacgccctag tgaataggca gaaaaatagt gcatccagct gtgatagtaa
    15541 tggtgagctc gtgcatagac gcgcgattgc agccataaac tctcttcttc cgtcagccag
    15601 ttgttgagca caccggcccg tgacagaaat cccattcgaa cataatccca ggctttgatt
    15661 cccccttctc cacagcacat agcggtttgg gcaacgaatt gggtataaat caaccctctc
    15721 tccgagccac cagcaattaa tgctttccac tcttcagggg aagagcgttg ccatgtcaga
    15781 tataagccag ctaaatcatt ggcatgacca tcatcagtca tctgataaat agtctgaatt
    15841 aaatcatcac gattatttat tccccaggat gatgagagat cgaattcttt tatcccggta
    15901 tacatttttg gcgagtcatg cgcgggtttg caactctgat taagtatttc catgggcgca
    15961 gaaagcgcaa aaagaagata ttgttcttcc ttattcattt tttaataatc ccttaagtgc
    16021 attaactgac tactttttta ttattgatga gtataaactt gacgctttca aaataaaaag
    16081 aaaatcgaag cattcacaca tgaataaaaa attaatgtat atattcgcaa tttttatagt
    16141 tgcagcaatt acctgtatta gccaacccaa gaaaacgatg ttgcgtgata aagccatggt
    16201 gaattatgcc tttgattatt taagatcacc aggcagtcta ccgttaacca cggcagccac
    16261 ggagctttcc gcgattcatg gtcactcaac atcgcaatat cgccttggcg aattttatct
    16321 tcatggtagc gacggtaaac cactggatta tacacaggcg agatactggt atgagcaatc
    16381 agcagaacag gaaaatccgc gcgcacaaag caaactgggg tggatctacc tgaaaggcct
    16441 gggagtcaaa cccgataccc gtaaagcaat tctctggtat aaggaagcag ctgaacaagg
    16501 gtatgctcat gctcaatata ctttgggttt gatctacaga aatggcactg gtattaatgt
    16561 taaccattat gaatctcaaa aatggttaaa actggccgcc aaacaacatt acaaaaatgc
    16621 ggaaagatta cttgccggac ttcccgcaca ttaacgtcga ctgtcacccg aagagatgga
    16681 gtattttccc tatcaaaccg gcaaagataa aaatgtagaa aataattttg atgacgccca
    16741 gacagcccag cccgccatca tcaacgggca ttggctgcgg ttgcggctcc ggctcgacgg
    16801 tttcctgacc ggtaaaattc gccgcctgaa ttaataatcg acgaataatt aatgcttcca
    16861 gtggacaacg atcttctgca tcaaggcggc tcacaatcgg cattaatgat tcttctgtcg
    16921 gttggctggc ctgcactaat tgtttggcaa ctaaatataa tggccataag ggatgttctg
    16981 tatcacgcgt gagtgagatc cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg
    17041 aacgccccag cgcagagagt ttttcatcgc tgagtatttc gtataaatga aaacgcgtac
    17101 gggcgctgtc gccggggaca taaagtagcg gttttccctg atcgttaacc agatcacgcg
    17161 cttcaatcat catcaccact aatgggtaat aatagacccg atgctgacgg caccattgca
    17221 acattaaagc atccgcctgg gtggagggat actcgcgcca gtagtaacaa agttctgcca
    17281 gcaggctttc gccttctcca caatagacgc gttgtgcgta ttcataataa cgcgcatctt
    17341 cattatcagg ttcagcttcc cgccacgacc gggcgtaatc caggagttcg gggattccca
    17401 ggcggcttgc gctataccag cggagtaaat ttctgtgcag ttttgcatca tcggggataa
    17461 tcactgcgcg atgttgcgct aaatacaatg tcagccattc tggatgcgat gaccaaatcc
    17521 gagcgaacat ttggtaaaaa tcgatggtgt cattttgcac ggcaactggc agatgcagaa
    17581 tggtctggaa gttaaaaaca ttccccttcg caatggcata taaaaaggat tccagttctt
    17641 cttcgtctat ttccccgacg ttttcctcct gcaaccaccg caatctttct gctaataaca
    17701 ccacgcactc gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta
    17761 attcatcaat ctcttccatc gagcaataat ttaattgctg aatgaatcgc tgccaggcgg
    17821 agggcagaaa acgttcgcta tcagaggcaa gtaacgcgcg aaaggcgagc agaatttcat
    17881 gttctgctac ctcatattct tctggttgcc aaacagattt agcaggcgac tgcgcaatcc
    17941 gtagcgcctc ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat
    18001 gaaaggacgg taacagcgcc agataagcgc ggcggataat atcgacgtca gtcgtttcct
    18061 ctatatcgag gatcttccag caatttttca taagtcgctc atatccttaa gcgtttccgg
    18121 gcatgtgggg tagtcagcca gtggttgcca ggacaaggta gggtaatacg catcatcact
    18181 ggcaaataat tgataaaaca tatcattgcc ggaatcgtcg tattctttac gcgctaagaa
    18241 ttcgcgtaat aaaggcagat gatcatcttc gggagactgc caatattgcc gaccaaaggt
    18301 ataaccggca aagtattgcc gccagttact ataataacgt agagcccgta gatgaatacg
    18361 cgactggatc catagacttt cttcttcact caaccagtta ttaagtacgc ccattcgact
    18421 taaaaaaccc atccggacat aatcccatgc tttaattcct ccctcaccac agcattcggc
    18481 tgtgctggcg acaaattgcg cataagcctg accttgctca tttaattcag caagatagtc
    18541 acgccattca catggagaat aacgaaacca gcggtgataa aaccctgcca accgagcggc
    18601 atgaccgttc tcgatcattc ggtgaattat gcgcagcagg tcatcacggt catcaattcc
    18661 ccatgaggaa gaaaggtcaa aaaagcatgt acctaaatac attttggggg atgaatgcga
    18721 aggtaaacag cattcattgc gtatttccat cggagtggaa agtgcaaaca atattttttg
    18781 cgaccccatg tccatataat ttatccttaa acattcattt aatgaatgta aactacattt
    18841 cgaggctcag ttatttatac ttttctgacg caacataata cgcataaaat aaggcttacg
    18901 caaaaatgga acacataaaa atataatata ttttatctac aaccataagg aaatgataat
    18961 gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga
    19021 aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat
    19081 tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgctg agcaaggtca
    19141 ttgtgaagca cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga
    19201 taatgagcaa gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc
    19261 caacgccctt ggctggatac tggatcgtgg agaagacccc aactataaag aagcggttac
    19321 ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc ttggatggat
    19381 gtacagaaat ggcaacggag tcgcacaaga ctatacgctg gcatttttct ggtacaaaca
    19441 agctgcatta caaggccata gttacgcaca agacaatctg gccgatcttt ataaagacgg
    19501 agaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa gcgcacagca
    19561 gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg aaggggtgga
    19621 ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg aaagcgtcgg
    19681 cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca
    19741 ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa
    19801 cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga
    19861 cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac
    19921 tgaagcttta ctgtagaaat aaaaacaagg gccggggaat ttcattctgc ccatattctc
    19981 tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat
    20041 aaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac
    20101 atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat
    20161 gattgaggtc ctggaaaatt tcgcgccatt caagctgcgt tttatcggtg atgtctgcca
    20221 gctcctgcca caagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca
    20281 ggctctcttc atagctcatg gcgaactctt ccaggtcacg tctgacagct cgcgtcagct
    20341 catcgatctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca
    20401 ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gttcagtgac gcgaccagtt
    20461 cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata
    20521 ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg
    20581 tacgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac
    20641 gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg tagccgtatt
    20701 gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga tagaatccaa
    20761 agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg agagcaaaga
    20821 gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca tgggccacgt
    20881 acgtaactac accatcggtg acgtgatcgc ccgttaccag cgcatgctgg gcaaaaacgt
    20941 tctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg cggtgaaaaa
    21001 caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa accagctcaa
    21061 aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc cggaatacta
    21121 ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat ataaaaagac
    21181 ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac aagttatcga
    21241 cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc agtggtttat
    21301 caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg atcactggcc
    21361 agacaccgtt aaaaccatgc agcgtaactg gatcggtcgt tccgaaggcg tggagatcac
    21421 cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc cggacacctt
    21481 tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga aagcggcgga
    21541 aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag ttgccgaagc
    21601 tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg ttcacccatt
    21661 aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt acggcacggg
    21721 cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct ctaaatacgg
    21781 cctgaacatc aaaccggtta tcctggcggc tgacggctct gagccagatc tttctcagca
    21841 agccctgact gaaaaaggcg tgctgttcaa ctctggcgag ttcaacggtc ttgaccatga
    21901 agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg agcgtaaagt
    21961 gaactaccgc ctgcgcgact ggggtgtttc tcgccagcgt tactggggcg cgccgattcc
    22021 gatggtgacg ctggaagacg gtaccgtaat gccaaccccg gacgaccagc tgccggtgat
    22081 cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag atccggagtg
    22141 ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt tcgacacctt
    22201 tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag aaggtatgct
    22261 ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg gtatcgaaca
    22321 cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg atgcaggcat
    22381 ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc
    22441 cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg atgctatcgt
    22501 tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt
    22561 ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat
    22621 ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt ctccggctga
    22681 tatgactctc gaatggcagg aatccggtgt ggaaggggct aaccgcttcc tgaaacgtgt
    22741 ctggaaactg gtgtacgagc acacagcaaa aggtgatgtt gcggcactga acgttgatgc
    22801 gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaactatcg ctaaagtgac
    22861 cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta tggagctgat
    22921 gaacaaactg gcgaaagcac cgaccgatgg cgagcaggat cgcgctctga tgcaggaagc
    22981 actgcaggcc gttgtccgta tgctcaaccc gttcaccccg cacatctgct tcacgctgtg
    23041 gcaggaactc aaaggcgaag gcgatatcga caacgcgccg tggccggttg ctgacgaaaa
    23101 agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag tccgtgccaa
    23161 aatcaccgtc ccggtggacg caacggaaga acaggttcgc gaacgtgctg gccaggaaca
    23221 tctggtagca aaatatcttg atggcgttac tgtacgtaaa gtgatttacg taccaggtaa
    23281 actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct ggcaacactg
    23341 ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg tgacaccacg
    23401 caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa cgggccatta
    23461 agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga taaagaaacc
    23521 acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca
    23581 tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt gaatgcgacc
    23641 gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg ttcattcttc
    23701 gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt aaaagagatg
    23761 tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc tgcggatatt
    23821 cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc
    23881 tccaccatgc tgggtaactg atgattcggt tgtacccgga acaactccgc gcgcagctca
    23941 atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta ttgcaggaaa
    24001 gccaggacgc tgttcgccag gtagctgcgg cacaaggatt cgaagaacac cacacttttt
    24061 ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct atgagtctgt
    24121 ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat gcggcgatca
    24181 atgaacaact tctcacactc accggacttc tgcatgacga cctgctattg atcgtccgcg
    24241 gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgctctt gcgaatcgca
    24301 gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg gttgctgtgc
    24361 gcgcaaaaca gctcaactta gaactggatg acgcggcgaa tcaggtgctc tgctactgtt
    24421 atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg ctctggccag
    24481 acggcaaact gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc
    24541 cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc
    24601 agcaactgcg tctggaaggc agcgagccgg ttattttgtt gcgcacatta caacgtgaac
    24661 tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg
    24721 ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa
    24781 gtcagccgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc accctcaaac
    24841 aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg ttgtgccata
    24901 aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc tctgtttggc
    24961 ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg
    25021 attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg tccccagccg
    25081 gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga caagccatta
    25141 tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc gcaaacactg
    25201 aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat tggtcaggat
    25261 tcattgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa tgcacatttg
    25321 atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata ccagcaatgg
    25381 ctggaagatc atttgacaca taacccggaa gatcttcacc ttcagcctgc cggtaaaatt
    25441 tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga acgtttgcaa
    25501 aacggtgaat cgtgtgagga tttattgccg gaaccggtat tgacttacat taaccaacaa
    25561 ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc
    25621 atggcgaaac acaagcgaat gtcgatggtc tgtacagcgg tcatgcgccc acacccctga
    25681 ccgcacgcgg tattgagcaa gcgcaaaatc tgcatacact gctagatgat gtttcctttg
    25741 acttggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg gttctcagtg
    25801 accgccagct ccccgttcat atcatccctg aactcaacga aatgtttttt ggcgactggg
    25861 agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc gcgtggtgca
    25921 atgactggca gcatgctatc cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg
    25981 tggaacgctt tatcgcaagg cttagtgaat atcagcacta tcagaatatt ttaatcgtca
    26041 gccatcaggg cgtactgagt ctgttaattg ctcgtttaat tggcatgcct gccgagtcta
    26101 tgtggcattt tcgcgttgac caggggtgtt ggagcgccat tgatatcaac caaaaattcg
    26161 cgacgctacg cgtcctcaat agccgtgcca tcggggtcga gaacgcatga cttttctgtt
    26221 tttttacggg taagccgcaa cgaccattga cagccccggg caggctgata ttctccgcag
    26281 ccagactttt tccgccagac acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa
    26341 tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg acaattgtcc
    26401 cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg attcacccag
    26461 ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat tgatgacctc
    26521 aaaggtcagg acatcatcgc cttagacgtt caaggcaaat ccagcatcac cgactgcatg
    26581 atcatctgta cgggtacgtc cagccgtcat gttatgtcca ttgctgacca cgttgtgcag
    26641 gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg
    26701 attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg
    26761 tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg
    26821 aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt tccgaaagat
    26881 atgcccttcg agctgattga aattccggcc ggcaaacgcg gcaagaatgc ggacatcaag
    26941 cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc
    27001 accctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga gctggaacgc
    27061 tggaagctgg atggtcgcga cgtaagtcta ctgattggcg ggcctgaagg gttgtcgcct
    27121 gcctgtaaag cggcggctga gcagagctgg tcgctgtcgg cgcttaccct cccccatccg
    27181 ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac caccaaccat
    27241 ccttatcacc gtgagtgata agggagcttt gagtagaaaa cgcagcggat gaaactacag
    27301 aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc
    27361 tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa tctgcaaatt
    27421 gttcgcttta ccgactacca gacccgctct aatgaaaacc gcattaagct ggtgcctatc
    27481 gcgcccagcc gcggcattat ctacgaccgt aacggtatcc ctctggccct caaccgcact
    27541 atctaccaga tagaaatgat gccggagaaa gtcgataacg tgcagcaaac gctggacgct
    27601 ttgcgcagcg tggtagatct gaccgatgac gatattgctg cattccgaaa agagcgcgca
    27661 cgttcacacc gtttcacctc tattccggtg aaaactaacc taaccgaagt acaagtagct
    27721 cgctttgccg tcaatcagta ccgttttccg ggtgtcgaag ttaaaggcta taaacgtcgt
    27781 tactatcctt acggttcggc gttgacccac gtcatcggct atgtgtcgaa aatcaacgat
    27841 aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc aacgcatgat
    27901 attggtaagc tgggcattga gcgttactat gaagacgtgc tgcacggtca gaccggttat
    27961 gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg
    28021 caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata tattgaaacg
    28081 ctgctggcgg gtagccgcgc agctgtggtt gttaccgaac cgcgtacagg tggggtgctg
    28141 gcgctggttt ccacgcctag ttatgaccca aacctgtttg ttgacggtat ctccagcaaa
    28201 gattattccg ccttgctgaa cgacccgaat acaccgctgg tgaaccgcgc cacacagggg
    28261 gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt aagcgccggg
    28321 gtgatcacgc gcaatacgac gctgtttgac ccaggctggt ggcaactacc agggtcggaa
    28381 aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg
    28441 gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat cgatcgcctc
    28501 tccgaatgga tgggtaaatt cggttatggt cattacaccg gtatcgacct ggcggaagaa
    28561 cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat
    28621 cagggtgaca ccattccggt tggtatcggc cagggttact ggacagcgac cccaatccag
    28681 atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc gcatttgctg
    28741 atgagcaccg ccgaagacgg caaacaggtg ccatgggtac aaccgcatga accgcccgtc
    28801 ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac
    28861 cgccctaatg gtacggcgaa taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa
    28921 tctggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt
    28981 gccgagcgtt tacgtgacca caaactgatg accgcctttg cgccatacaa caatccgcaa
    29041 gtggctgtcg ccatgattct ggagaacggc ggcgcgggtc cggcggttgg tacgctgatg
    29101 cgccagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc tgcggaaaat
    29161 ccagcggttg ccgcagcgga ggaccattaa tcatgacgga taatccgaat aaaaaaacat
    29221 tctgggataa agtccatctc gatcccacaa tgctgctgat tttactggca ttgctggttt
    29281 acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa
    29341 tcggccaaat cgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg
    29401 tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg gtggcggtag
    29461 atgctttcgg agccatctct aaaggcgctc aacgctggct ggacctcggt attgttcgtt
    29521 ttcagccgtc ggaaattgcc aaaatagccg taccactgat ggttgcgcgc tttatcaacc
    29581 gcgacgtttg cccgccatcg ttgaagaata ctggcatcgc gctggtgctg atatttatgc
    29641 ccacgctgct ggtggctgca cagcctgacc tgggaacatc aatcctcgtt gcgctttccg
    29701 gtctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc gcagtagtgc
    29761 tggtagcggc gttcattccg attctgtggt tcttcctgat gcatgattac cagcgccagc
    29821 gcgtaatgat gctcctggac ccggaatcag acccactcgg cgcgggctat cacattattc
    29881 agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg cacggcactc
    29941 agtcacagct tgaatttctc cccgaacgcc atactgactt tatcttcgcg gtactggcgg
    30001 aagagctggg attagtgggc attctgattc tgctcgctct ctacattctg ctgatcatgc
    30061 gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg gctggtggct
    30121 taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc ggtattctgc
    30181 cggttgtagg ggttccgctc ccactggtca gttatggagg atcggcgcta attgtgctga
    30241 tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg
    30301 tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc aggaatgctc
    30361 gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta
    30421 tgtaacggcc ccatagttga aattagcggg gcggacccgc gtttcgaacc actgaacgcg
    30481 acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct
    30541 cgatttagcc aggcgggact ggcggcaatc tatgatgccg aaccaggcag taacctgacg
    30601 gcctctggcg aagctttcga tccgacaaag ctgacggcgg cccatccaac gcttccgatc
    30661 cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt gcgcattaat
    30721 gatcgcggcc cttacggcaa cgaccgcgtt atttcgcttt ctcgcgcggc agctgaccgt
    30781 cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc ccaggatggt
    30841 tcgctttctg gtcctggtat ggcttgtacc acagtcgcca aacagactta cgccctgcct
    30901 gcacctcccg atttaagcgg tggcgcggga acaagttcag tgtctggccc gcagggtgac
    30961 attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc
    31021 agtagcggtt tcctcggcgc tccaacgacc ttagcgcctg gtgtactgga aggcagcgaa
    31081 ccgacgcctg cgccacagcc cgttgttaca gcttcgtcga caacgcctgc aacctcgcct
    31141 gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc
    31201 ggggccgtaa gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg acagaagttc
    31261 ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct tggcccattc
    31321 gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc ccaattacag
    31381 tcatttatta ctaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa
    31441 tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt tgctatagta
    31501 gggcactttt ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc
    31561 gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct
    31621 gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg
    31681 gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat
    31741 gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca
    31801 atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc
    31861 accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt
    31921 ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc
    31981 atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa cagctacgtt
    32041 aacgcactgg gcctgaaaaa tacccacttc cagacggtac atggtctgga tgctgatggt
    32101 cagtacagct ccgcgcgaga tatggcgctg atcggccagg cattgatccg tgacgtaccg
    32161 aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac
    32221 cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact
    32281 gacaaagcag gttacaatct tgttgcttct gcgactgaag gccagatgcg cttgatttct
    32341 gcggtgatgg gtggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc
    32401 tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga gttcgcctct
    32461 gaaccggttt ggtttagtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac
    32521 ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct gaacagcagt
    32581 gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat
    32641 ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc
    32701 ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg
    32761 aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa aactcccgcc
    32821 ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa accaaactta
    32881 acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag gcgttacctg
    32941 agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac tacaccccaa
    33001 cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc aacgccactc
    33061 atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg
    33121 ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct ctcctcgctg
    33181 tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt gtatcaggat
    33241 aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctcccg ggctatgcat
    33301 gaattcaccg atacccgcga taatagtacc cttgatgaaa tctggctggt cgagcactat
    33361 ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt
    33421 ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg acaacaggtg
    33481 atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg
    33541 cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca tcctcgggct
    33601 gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt acgaattcga
    33661 cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc accattttta
    33721 cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca atggaaaccc
    33781 gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc gctactaaac
    33841 aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca atttacactg
    33901 ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt atattttctt
    33961 tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat tgattaacct
    34021 attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca taagattatt
    34081 gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt ggatagtaat
    34141 aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca aatatttaca
    34201 accttacgaa atattgatct taaccttctg actatttttg aagctgtata tgtacataaa
    34261 gggatcgtta atgcagcgaa agtgcttaat ctgaccccct cggcaatcag tcagtctatt
    34321 cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca gggtgtcact
    34381 cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga gtccattctt
    34441 ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat tgcgaccact
    34501 ccctcggtcg gagccttggt ccttcctgtc atctatcggg cgattaaaac tcactatccg
    34561 cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag tcagtttcaa
    34621 accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca tcatgttctg
    34681 ttcaccgaca atatggtgtt aatttgccgc aagggaaatc cactactctc tttagaagat
    34741 gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaagg gcaaaatttc
    34801 agcggtctgc ggcagagagt tcaagagatg tttccggacc ggcaaatcaa tttcaccagc
    34861 tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc gattattccc
    34921 agccgttttt ataacctgtt tagtcgttgc tggccgctgg aaaaactgcc ttttccgtcc
    34981 ttaaatgagg agcaaataga tttctctatc cactacaaca aattcatcct gcgtgatccc
    35041 atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat tgttacagcc
    35101 agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca ttttcgcgac
    35161 cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata
    35221 gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa
    35281 cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga
    35341 taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa
    35401 gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc
    35461 cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc
    35521 ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt gtacccgccg
    35581 ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt
    35641 gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga
    35701 ccgtgatgac ctgcgcgatg gcggtgccca acactttgcg gattgtatta ctgccattcg
    35761 ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgcg gtcgaatgga
    35821 tcgtgctctg gatattctga ctgcaacgcc cccagatgtg ttcaaccata acctggaaaa
    35881 cgtaccgcgt atttaccggc aggtacggcc tggcgcagat tacaactggt cgctgaagct
    35941 gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg
    36001 tctgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt
    36061 gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg
    36121 ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac
    36181 ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg
    36241 aatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa aacccgcttt
    36301 ttgaagcggg ttttttgtat cgaacaagat attgagggag cgtcctgctc gccacgtcac
    36361 tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt cgccgcagca
    36421 tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc tccgcccagc
    36481 gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac cagcagtttg
    36541 gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca gaacgcgcct
    36601 taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa
    36661 ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca aagcgacggt
    36721 tgtttaagat gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca
    36781 taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga tctataatcc
    36841 ggctttgccc gatatttttg ttcccgcact cccccgccgc caccatataa caggtggtat
    36901 ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc ccacgaaccc
    36961 aggcggcagg aagtaccagg atttcagctc cctgtaaagc ctgtgccagc gccagctctg
    37021 gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc tccagtaacg
    37081 gagctatttc attaccagca tcaacatggc gtgattcctg aatggcaaat gcatcataga
    37141 gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc agcatattcc
    37201 atgcgcgccc cggcgttgaa ggaagatgaa tcgtcagaat tgtcgtcatc atgttacgtt
    37261 tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct gatttaaccg
    37321 atagatctgc atcatgatca tcgcgcgcca gcaatgcttc cggcaggaca aacagcgata
    37381 cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc ttttcccaca
    37441 cagatgtaac agcaaactgt cctgcagcaa ccagcattgc cacctcctgt cagagtcgta
    37501 aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat caaatagcag
    37561 gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac gggaagcgta
    37621 gcgagatggc tgttaagtat gcgatttaac ccgctgcatc aggcgattcc gttggggacg
    37681 ctggcagcaa acctgattgg ggcattcatc ataggaatgg gattcgcctg gttcagcagg
    37741 atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg tggcggtcta
    37801 acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc
    37861 tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac cgcactggca
    37921 ttctggctgt tttcggcctc aaccgtacac taaagaccaa aaaaaacccg ctgattaagc
    37981 gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct
    38041 ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca
    38101 ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg
    38161 aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa
    38221 attaccttta catgaaaaat agacacaaat gctgtgtcgg ttatcagtac accaattgtg
    38281 gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt
    38341 tgcttatttt agttattgat tttaaagaag ttactaaaac ttcatttgtc tcaaaactga
    38401 aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc
    38461 tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg gagagggatg
    38521 taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa atgaaaattt
    38581 tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg
    38641 ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt ttcatcccaa
    38701 cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt tttttctttg
    38761 tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata atgttcaggc
    38821 tgttgccagg tttgtgcaat attttctcta aacgttgtca tcctctcatc tgcgttagca
    38881 aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc gacatattta
    38941 ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc atagcaaagc
    39001 tacaattaac ataaccttaa tagacccaac ataaagaata atctgaatag ctgctctatt
    39061 tatttccgct tgttcaagtt aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt
    39121 ctgcgatggg aatagtcaaa aaagaaaaac caagtctttt ttgatgacaa aatgcaatca
    39181 aggaaaaatt attttatttt tttaacgaga tacaacaatc atcttaacga agtatataat
    39241 atatatcgtc aacccatgct catattagat tattttttga gcgcacagcc gcagctaaca
    39301 ccattgcaat taacaaattt gcatcaatcc accatcaatt tacacacatt attatgtgat
    39361 aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagca acttcaatgt
    39421 cttaatggca gtttttcttg attttaatca gcattcatcg ccaatttatt gggcatattt
    39481 tttccttaag ctttaggaag tttttattat ttactttggg cctggagaca ggaaaaatta
    39541 tgctgacatt cattgaactc cttattgggg ttgtggttat tgtgggtgtg gctcgctaca
    39601 tcattaaagg gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta
    39661 tcagtgccat tatggggcac aaagtgttac cgtccagcca ggcttcaaca ggctacagcg
    39721 ccacggatat cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca
    39781 tgatgattat gatgctgtgt ggctttgccg cttacatgac ccatatcggc gcgaatgata
    39841 tggtggtcaa gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga
    39901 ttgccgccta ttttgttgct tgtctgatgt cactggccgt ctcttccgca accggtctgg
    39961 gggttttgct gatggcaacc ctgtttccgg tgatggtaaa cgttggtatc agtcgtggcg
    40021 cagcagctgc catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg
    40081 tggtgctggc ggcgcaggct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc
    40141 tgcctatctc aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt
    40201 atctggataa aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca
    40261 ctgcccccgc gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg
    40321 acggtaaatg gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg
    40381 cctccattct ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag
    40441 tggcttatcg cgggatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg
    40501 gggtattcgc tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta
    40561 cctcgtttgg ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg
    40621 cggcagtcac gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga
    40681 aactggcgca ctcttccggc attaacccgg cgtatttgac tattccgatg ctgcaggcgt
    40741 caaacctggg tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg
    40801 cgaaaatctc accatttgaa gtcgtaaaac gcacctcggt gccggtgctt gttgggctag
    40861 tgattgttat cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacaggca
    40921 agtaaatagt aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta
    40981 tatgcgttgt ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct
    41041 ggcgcaatat tcaagataac gcctggcagt ggtgcggctg atggtcagtg cctgcgccac
    41101 cgtttctgcc gtatgttgca caccaggctc tttaaacagt tttcgcaccg cgtttagcgt
    41161 caaggggtca atgccggtcg gcagttcgtc tttgggttca ccgcgagcat aggcattaaa
    41221 catctcatca atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg
    41281 gcggaaacgg gttagcgttt gccccagtgt attgattcac ttgaagtacg aaaaaaccgg
    41341 gagaacattg gattattcgg gatctgatgg gattagattt ggtggggctt gcaagcctgt
    41401 agtacaaatt ttaggcgtta accaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt
    41461 tcattgaaaa aacgaaaaac aaaaacacta taaatcattt caatcatagc ttcaaaaaac
    41521 gttcaaaacc gataacaacc aggctgtcat caaatgactc atatcacaaa tcagctcatg
    41581 ccgtttaggt atgttacatg tgtgattatg tgaggtgaag tatgttttag ctggctcatg
    41641 gttgttatac ggcttttttc gcatcttgtg gttccaacaa aggagctaca ggctcttcct
    41701 gtttatgaac accatacatt gacaaaatct cttggcgagt ttctggaggt aatttatgca
    41761 gcgccaaagc tgtttgtagt gtttggcgat caatacccag cgttgccaat agggtgtcaa
    41821 cggagatgga ggggcgttct tgcattaatc cggaaagccc atatttagcc atgtttagct
    41881 ctatctctcg gacttcattt tctcctagtt tattgattaa cagggagaac attttcaatg
    41941 tatcagcatt catttcttcg atagcaattt tttgcatagc cggtattgcc gggatatatt
    42001 caaccccagg cccgcgacgc ccttcttgtg aacgcttcgg ccatccttct ttctttgctc
    42061 gtttatgcac cccctgtatt gtcccaggca ttccatccag ttcagtaatt tgtttgggtg
    42121 tcatccactg gtttatctca tttgcgcttt tcataaacta agtttaccag atttatctat
    42181 ataaaaatca atggattaca aaataaacca cattaactga tataaaccgg tttggtttat
    42241 ttggtaatgt ataaaacaga taatcaagac gaattatttg ttttggtaac cctttatcat
    42301 acgcaatatg gttggtgtgc gaaagatgag taacgattgg catcgggccg acgtgattgc
    42361 ggccctcaaa aaaacaggaa cccctctggc aaaattgtcc agacgttcag gttattcaac
    42421 cgggacgctt gcaaatgcat tagtcaggcc atggcctaaa ggtgaacgca ttattgctga
    42481 cgccttaaac atgaaacctg aggaaatttg gcctagtaga tatctaaggg atcacaatga
    42541 gagagtggat atcagcaatt gaatgcatgg gtttatctgg tttacctggt tcattaatgg
    42601 gggttcacaa aagagctcag agagaaggat ggctcaagag aagaagagaa ggaattagag
    42661 gggttgctta cgaataccat atatcttctc ttcctaacat tgctcgagct gaagttttgc
    42721 tccaacaagg aaaatttaaa accagtcagg gatgttttga aatcgcccgc cccacgctgg
    42781 aagctcatga ttatgatcgt gaggcactat ggagcaaatg ggataaagcc agcgattccc
    42841 agcgcagcct ggctgaaaaa tggttgccct caattcaggc cacagacgaa atgctgaacc
    42901 aggggatttc aacgaaaacg gcttttgcga ccgttgcagg gcattaccag gtcagcgcat
    42961 ccactttgcg ggacaagtat taccaggtac agaagtttgc gaagcctgac tgggcggctg
    43021 cacttgttga tggacgtgga gcatcccgtc gcaatgttca caaaagtgaa tttgacgagg
    43081 atgcctggca gtttctgatt gcagattatc tgcgaccgga aaaacccgcc ttccgcaaat
    43141 gttatgagcg tctggaactg gcagcccgcg agcatggctg gagtattccc tcccgtgcca
    43201 cggcctttcg ccggattcag caactggacg aggcaatggt tgttgcctgt cgtgaaggtg
    43261 aacatgcact gatgcatctg ataccggcac agcagcgaac tgtggaacac ctggacgcca
    43321 tgcagtggat caacggcgac ggttatctgc ataacgtctt tgtacgctgg tttaacggtg
    43381 atgtgatccg tccgaaaaca tggttctggc aggatgtgaa aacccgaaaa attctgggct
    43441 ggcgctgcga tgtgagcgag aacattgatt caattcgcct ctcgttcatg gatgttgtga
    43501 ctcgctacag tatcccggag gattttcaca tcaccattga taacacccgt ggtgctgcga
    43561 ataaatggct gacgggaggc gcgcccaatc gctaccgctt taaggtaaaa gaggacgatc
    43621 caaaaggact gtttttactg atgggtgcga aaatgcactg gacaagcgtt gttgccggta
    43681 aaggctgggg ccaggcaaaa cctgttgaac gtgctttcgg tgttggtggg cttgaggaat
    43741 acgttgataa gcatccggca ctggctggcg catatacggg gccaaatccg caggcaaaac
    43801 ctgataacta tggcgaccac gctgttgatg cagagctgtt tctgaaaacc tttgccgaag
    43861 gtgtggcgat gttcaatgcc agaacaggcc gtgaaacaga aatgtgcggg ggcaaactct
    43921 cgtttgatga tgttttcgag cgtgaatacg ccagaacgat tgtgcgtaag ccaaccgaag
    43981 aacaaaaacg gatgctgtta ctgcctgccg aggcggtgaa cgtttcacgc aaaggcgagt
    44041 ttacgcttaa agttggcggc tcccttaaag gcgcgaaaaa cgtttattac aacatggcat
    44101 taatgaatgc cggagtgaaa aaagttgtgg tcagatttga tccacagcag ttacacagca
    44161 cggtttattg agatag
//
LOCUS       DAJTRM010000024        42246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRM010000024 DAJTRM010000000
VERSION     DAJTRM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.024"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..242)
                     /gene="ssb1"
                     /locus_tag="OO322_001409"
     CDS             complement(<1..242)
                     /gene="ssb1"
                     /locus_tag="OO322_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5906347.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYI"
     gene            496..3318
                     /gene="uvrA"
                     /locus_tag="OO322_001410"
     CDS             496..3318
                     /gene="uvrA"
                     /locus_tag="OO322_001410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5906348.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3353..3709)
                     /locus_tag="OO322_001411"
     CDS             complement(3353..3709)
                     /locus_tag="OO322_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5906349.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3713..4129)
                     /locus_tag="OO322_001412"
     CDS             complement(3713..4129)
                     /locus_tag="OO322_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5906350.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4240..4953)
                     /gene="aphA"
                     /locus_tag="OO322_001413"
     CDS             complement(4240..4953)
                     /gene="aphA"
                     /locus_tag="OO322_001413"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5906351.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5355..5559
                     /locus_tag="OO322_001414"
                     /pseudo
     CDS             5355..5559
                     /locus_tag="OO322_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6062..7255)
                     /gene="tyrB"
                     /locus_tag="OO322_001415"
     CDS             complement(6062..7255)
                     /gene="tyrB"
                     /locus_tag="OO322_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5906352.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7508..8587)
                     /gene="alr"
                     /locus_tag="OO322_001416"
     CDS             complement(7508..8587)
                     /gene="alr"
                     /locus_tag="OO322_001416"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5906353.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8640..10055)
                     /gene="dnaB"
                     /locus_tag="OO322_001417"
     CDS             complement(8640..10055)
                     /gene="dnaB"
                     /locus_tag="OO322_001417"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5906354.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10138..11121
                     /locus_tag="OO322_001418"
     CDS             10138..11121
                     /locus_tag="OO322_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5906355.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11287..11529)
                     /gene="pspG"
                     /locus_tag="OO322_001419"
     CDS             complement(11287..11529)
                     /gene="pspG"
                     /locus_tag="OO322_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5906356.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11663..12700)
                     /gene="dusA"
                     /locus_tag="OO322_001420"
     CDS             complement(11663..12700)
                     /gene="dusA"
                     /locus_tag="OO322_001420"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5906357.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13063..14048)
                     /locus_tag="OO322_001421"
                     /pseudo
     CDS             complement(13063..14048)
                     /locus_tag="OO322_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14366..14881
                     /gene="zur"
                     /locus_tag="OO322_001422"
     CDS             14366..14881
                     /gene="zur"
                     /locus_tag="OO322_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5906358.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(14923..15132)
                     /locus_tag="OO322_001423"
     CDS             complement(14923..15132)
                     /locus_tag="OO322_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5906359.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15248..16573)
                     /gene="dinF"
                     /locus_tag="OO322_001424"
     CDS             complement(15248..16573)
                     /gene="dinF"
                     /locus_tag="OO322_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5906360.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16646..17254)
                     /gene="lexA"
                     /locus_tag="OO322_001425"
     CDS             complement(16646..17254)
                     /gene="lexA"
                     /locus_tag="OO322_001425"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5906361.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17364..17732)
                     /gene="dgkA"
                     /locus_tag="OO322_001426"
     CDS             complement(17364..17732)
                     /gene="dgkA"
                     /locus_tag="OO322_001426"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5906362.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17903..20326
                     /gene="plsB"
                     /locus_tag="OO322_001427"
     CDS             17903..20326
                     /gene="plsB"
                     /locus_tag="OO322_001427"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5906363.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20481..21353)
                     /gene="ubiA"
                     /locus_tag="OO322_001428"
     CDS             complement(20481..21353)
                     /gene="ubiA"
                     /locus_tag="OO322_001428"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5906364.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21366..21863)
                     /gene="ubiC"
                     /locus_tag="OO322_001429"
     CDS             complement(21366..21863)
                     /gene="ubiC"
                     /locus_tag="OO322_001429"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5906365.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22086..23666)
                     /locus_tag="OO322_001430"
                     /pseudo
     CDS             complement(22086..23666)
                     /locus_tag="OO322_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(23895..24815)
                     /gene="malM"
                     /locus_tag="OO322_001431"
     CDS             complement(23895..24815)
                     /gene="malM"
                     /locus_tag="OO322_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5906366.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(24967..26307)
                     /gene="lamB"
                     /locus_tag="OO322_001432"
     CDS             complement(24967..26307)
                     /gene="lamB"
                     /locus_tag="OO322_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5906367.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26379..27494)
                     /gene="malK"
                     /locus_tag="OO322_001433"
     CDS             complement(26379..27494)
                     /gene="malK"
                     /locus_tag="OO322_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5906368.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            27859..29049
                     /gene="malE"
                     /locus_tag="OO322_001434"
     CDS             27859..29049
                     /gene="malE"
                     /locus_tag="OO322_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5906369.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29203..30747
                     /gene="malF"
                     /locus_tag="OO322_001435"
     CDS             29203..30747
                     /gene="malF"
                     /locus_tag="OO322_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5906370.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30762..31652
                     /gene="malG"
                     /locus_tag="OO322_001436"
     CDS             30762..31652
                     /gene="malG"
                     /locus_tag="OO322_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5906371.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPISI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32023..33498
                     /gene="xylE"
                     /locus_tag="OO322_001437"
     CDS             32023..33498
                     /gene="xylE"
                     /locus_tag="OO322_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5906372.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33542..33952)
                     /gene="psiE"
                     /locus_tag="OO322_001438"
     CDS             complement(33542..33952)
                     /gene="psiE"
                     /locus_tag="OO322_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5906373.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34078..34221)
                     /locus_tag="OO322_001439"
     CDS             complement(34078..34221)
                     /locus_tag="OO322_001439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906374.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34222..34500
                     /locus_tag="OO322_001440"
     CDS             34222..34500
                     /locus_tag="OO322_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906375.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34547..36643)
                     /locus_tag="OO322_001441"
     CDS             complement(34547..36643)
                     /locus_tag="OO322_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5906376.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNNLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(36643..37380)
                     /locus_tag="OO322_001442"
     CDS             complement(36643..37380)
                     /locus_tag="OO322_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5906377.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37377..38015)
                     /locus_tag="OO322_001443"
     CDS             complement(37377..38015)
                     /locus_tag="OO322_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5906378.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38129..38371)
                     /locus_tag="OO322_001444"
     CDS             complement(38129..38371)
                     /locus_tag="OO322_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906379.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(38834..40483)
                     /gene="pgi"
                     /locus_tag="OO322_001445"
     CDS             complement(38834..40483)
                     /gene="pgi"
                     /locus_tag="OO322_001445"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5906380.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41008..>42246
                     /gene="lysC"
                     /locus_tag="OO322_001446"
     CDS             41008..>42246
                     /gene="lysC"
                     /locus_tag="OO322_001446"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5906381.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPF"
BASE COUNT        10523 a        10859 c        10366 g        10498 t
ORIGIN      
        1 tcgatataaa cctgagaacc tttacgcaga tattcgctcg ccacttctgc cagtttgccg
       61 aacagcacaa cgcggtgcca ttcagtctgt tctttcatct cgccggtcgc tttatcacgc
      121 caggattcgg aagtagccag cgtaatgttg gcaactgcgc caccatttgg catgtagcgt
      181 acttccgggt cctgacccag attaccaacg agaataacct tgtttacgcc tctgctggcc
      241 atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt aaacgcgcga ttgtaccatt
      301 accaatagcg cttttactat gttgtgacct cggttccggg aaacaaacct ggccagacat
      361 tgttacacaa cactccggat aatgcattcc aatactgtat attcattcag gtcaatttgt
      421 gtcataatta accgtttgtg atcgccggta gcaccatgcc accgggcaaa aaagcgttta
      481 atccgggaaa ggtgaatgga taagatcgaa gttcggggcg cccgcaccca taatctcaaa
      541 aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg tgaccgggct ttcgggttct
      601 ggcaaatcct cgctcgcttt cgacacctta tatgccgaag ggcagcgccg ttacgttgaa
      661 tccctttccg cctacgcgcg gcagtttctg tcactgatgg aaaagccgga cgtcgatcat
      721 attgaggggc tttctcctgc catctcaatt gagcagaaat cgacgtctca taacccgcgt
      781 tctacggtgg ggacaatcac cgaaatccac gactatttgc gtttgttgtt cgcccgcgtc
      841 ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg cgcaaaccgt cagccagatg
      901 gtggataacg tgctgtcgca gccggaaggc aagcgtctga tgctgctcgc gccaatcatt
      961 aaagagcgca aaggcgaaca caccaaaacg ctggagaacc tggcaagcca gggttacatc
     1021 cgtgctcgta ttgatggcga agtctgcgat ctttccgatc cgccgaaact ggaactgcaa
     1081 aagaaacata ccattgaagt ggtggttgat cgcttcaagg tgcgtgacga tcttacccaa
     1141 cgtcttgcgg agtcgtttga aaccgcgctg gaactttccg gtggtacagc ggtagtggcg
     1201 gatatggacg acccgaaagc ggaagagctg ctgttctccg ccaacttcgc ctgcccaatt
     1261 tgcggctaca gtatgcgtga actggagccg cgactgtttt cgtttaacaa cccggcaggt
     1321 gcctgcccga cctgtgacgg ccttggcgta cagcaatatt tcgatcctga ccgcgtgatc
     1381 caaaaccccg agctgtcact ggctggcggt gcgatccgtg gctgggatcg ccgcaacttc
     1441 tattacttcc agatgctgaa atcgctggca gatcactata agttcgacgt cgaagcgccg
     1501 tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt acggttctgg caaagaaaac
     1561 attgaattca aatacatgaa cgatcgtggc gatacctcca tccgtcgtca tccgttcgaa
     1621 ggcgtgctgc acaatatgga gcgccgttat aaagagacag aatccagtgc ggtacgtgaa
     1681 gaattagcca agtttatcag caatcgccca tgcgccagct gcgaagggac gcgtctgcgt
     1741 cgggaagcgc gccacgttta tgtcgagaat acgccgctgc ctgctatctc cgacatgagc
     1801 atcggtcatg cgatggaatt cttcaacaat ctcaaactcg ctggtcagcg ggcgaagatt
     1861 gcggaaaaaa ttcttaaaga gatcggcgat cgcctgaaat tcctcgtaaa cgtcggcctg
     1921 aattacctga cactttcccg ctcggcagaa acactttccg gcggtgaagc ccagcgtatc
     1981 cgtctggcga gccagattgg tgcgggcctg gttggcgtta tgtacgtgct ggacgagccg
     2041 tctatcggcc tgcaccagcg cgataacgag cgcctgttgg gtacgcttat ccatctgcgc
     2101 gatctcggta ataccgtgat tgtggtggag catgacgaag acgcaattcg cgccgctgac
     2161 catgtgatcg atatcggtcc tggtgcgggt gttcacggcg gtgaagtggt cgcagaaggt
     2221 ccgctggaag cgattatggc ggtgcctgaa tcgttgaccg ggcagtacat gagcggtaaa
     2281 cgcaagattg aagtgccgaa gaaacgcgtt ccggcgaatc cagaaaaagt gctgaagctg
     2341 acaggcgcac gcggcaacaa cctgaaggac gtgacgctca cgctgccagt cggtctgttt
     2401 acctgcatca caggggtttc aggttccggt aaatcgacgc tgattaacga cacactgttc
     2461 ccgattgccc aacgccagtt gaatggtgcg accatcgccg aaccggcacc gtatcgcgat
     2521 attcagggac tggagcattt cgataaagtg atcgatatcg accaaagccc aattggtcgt
     2581 actccacgtt ctaacccggc gacctatacc ggcgtgttta cgcctgtgcg cgaactgttt
     2641 gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag gacgtttcag ctttaacgtc
     2701 cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga tcaaagtgga gatgcacttc
     2761 ctgccggata tctacgtgcc gtgcgatcag tgcaaaggta aacgctataa ccgtgaaacg
     2821 ctggaaatta agtacaaagg caaaaccatc cacgaagtgc tggatatgac catcgaagag
     2881 gcgcgtgagt tctttgatgc ggtgccagct ctggcgcgta agctgcaaac gttgatggac
     2941 gttggtctga cgtacattcg cctggggcag tccgcaacca cactttctgg tggtgaagcc
     3001 cagcgcgtga agctggcgcg tgagctgtca aaacgcggca ccgggcagac gctgtatatt
     3061 ctcgacgagc cgaccaccgg tctgcacttc gccgatattc agcaactcct cgacgtgctg
     3121 cataaactgc gcgatcaggg caataccatt gtggtgattg agcacaatct cgacgtgatc
     3181 aaaaccgctg actggattgt cgacctggga ccagaaggcg gcagtggcgg cggcgaaatc
     3241 ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag cttcgcatac ggcacgcttc
     3301 ctcaagccga tgctgtaatc gttaaggccg ctttctgagc ggccttttcc tttcagagtt
     3361 gtaccagcaa tttacgcttt tcttccggta gtaaattcac tgcctgctga taagacgcat
     3421 ccaccagata atagatttgc gaatccggca gcgaaccgtc gagatacacg gtgctccagt
     3481 gcgctttatt cagatggcgg ctaggacgca catcgctgtg ctgctgacgt agcagctccg
     3541 ccagctccgg gctggttttc agcgaaacag ctgggcgatt ttctacttct ttcaccatcg
     3601 caaacagtac atcttccact ttgatctgcg tcgctttcca gtcgttatgc acgctctgtt
     3661 ctgcgcttgg ttttgccatg caatattgta gcaactccga aatggtcatt ttttactccc
     3721 cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat gcgatgttcc cccagccaga
     3781 tgccttgcca agtgccggtc tgaatacgcc ctttatgtac cggcaataca agcgatgttc
     3841 ccagcattga ggatttgata tgagaaggca tatcgtctgc tccctcatag tcatgctcat
     3901 aatttccgtt gtcgggaacg gtgcggagga aaaaacactc catgtcgtgg cgtacggtgg
     3961 gatcgcagtt ctcattaagt gtcagagagg cggaggtatg ttgcagcaac agatgcagta
     4021 agccgatgtt aacgcgcggc atatcagcca gctgattcag aatttcatcc gttaccagat
     4081 gaaacccacg agatttggcg ctaagcgtga gcgtcttttg ataccacatg tgctgctcct
     4141 tgataaaact ctcttaatca gtttgcagca agacagcgaa aggataaagg tgtgattaaa
     4201 aaaacagcat tcgggagagc atcactctct cccgctctgt cagtattctg aattgacgat
     4261 cacctcttca ccaaacgcac ccgcttgtgg caagggtttg taggtagagt tggaggcgcg
     4321 cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg gtaatatcgt tatcggaatc
     4381 gccataaaaa attcggatat ttttatcctg cagccattgc gattttgtat tttgccctgg
     4441 tttatcgccc gcaaagatta ccggattcat gttggtggca ggaatatgaa aattatccgc
     4501 cagcgttttt gaaaccgttt ctgttttcgt cgggctacga ccagtcacaa agaagatcgc
     4561 gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg acctcttttg gaatgctgaa
     4621 ttcatcccag ccattattca ttttttccca gaacacagga tttttcagat aatcttcgct
     4681 ttctggcgag aaggtttttt tgccgcgcca gaaccccgga ctggaaaaaa gtaccgtgtc
     4741 atcgatatca aaccccaccg ccattggcgg acgccctgcg aggctatttt caatttgtgc
     4801 gaccgaaacc caatgaattg gtgcctgttc agcaagcctg gcaacgttag tcccagggtt
     4861 aagcggtgaa ggagatgagg ccagggcaac agcggaactg tttagagcga acaataagca
     4921 aacggcactg attgcctgtg tgatcttgcg catatttttc cctaaatagt cagtatgttg
     4981 aaacttttta ttgtgagatt tgttgcaaaa accatctgac cataacgcca gctgccgggg
     5041 aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg atgattatat ttaacccagt
     5101 aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt actcgatgta aggatacatt
     5161 aattttacgt ttattatgat gaatcctcgg caaatgagcg aaatttgata gaacatttat
     5221 ctgctgtacg atttaatgaa cttaatgata cagcattaca aaaacaatcg aagtttataa
     5281 agatgatttc tgattgacca gccccctacc ctacaatggt gattaatttt attactcgcg
     5341 atggagatga tgatatgaat atatcatatg tgaatagcaa caaaacgaca tctttacctg
     5401 ttgagctaga tgcactaaat aataaagata tctcttatgc caaagatttc tttttataca
     5461 tagaaacaca gttaaaaatt gcaaaagatt tttgtagacc tggagaagaa gtatcaagtt
     5521 ctattgcaag taaagttttt cacgcattta ttgatttagt taacaaaatc aggggtaaga
     5581 aagactttat gtatatttgc acgctttgct gttttgccga ggaggttaaa ggtgattatt
     5641 ctcattacag gaccttttta tttgatattg gtaatcaata taaggttaaa cttacacaaa
     5701 gcggaaaaaa gagttctctt taacattaga atttaacgat actataattg aatctcagaa
     5761 agtcacaggc aataaggcaa agcatattct tgaggatata gaaatattct atcgtaacaa
     5821 acccgatact tattattaaa tttaataatg gttagatgtg cagaaattat tactccacac
     5881 gaaaaaatag ggaaaggaat tccagataat tgggataaac ttaacaataa aagaactgac
     5941 aacgttttat tatctctcac gtagaacaac ggcatcaaaa aaatcatcta caaagaaata
     6001 gtgaaagcga cgacagttta atttcactgc aggctgggta actccagcct gctttcctgc
     6061 attacatcac cgcagcaaac gcctttgcca cacgttgtac atttgccgta tttaacccgg
     6121 cgacacacat gcgaccgctg gcgatgagat agacaccaaa ttcttcacgt agtcggtcaa
     6181 cctgagcggc acttaaaccg gtataactga acatgccgcg ctgattaagc agataatcga
     6241 aattgcgttc tggcatctct gtgcttaata ccttcaccaa ttcctgacgc attgccagaa
     6301 tgcgagtacg catctcttct acttccgcca gccagctggc tttcaatgcc tcgtcattca
     6361 gcactgcagc caccacctgc gcaccaaaat tcggcgggct ggagtagttg cggcgaactg
     6421 ttgctttcaa ttgccccagt acgcggccag cggcttcggc atcttcacac agaacagaaa
     6481 gtccgccgac gcgctcgccg taaagggaga aaattttcga gaacgaattg ctcaccagag
     6541 cgggtaatcc agcactggca atggcgcgaa tagcgtaggc atcctcttcc ataccggcac
     6601 taaatccttg ataggcaata tcgaggaatg ggataagctc gcgggctttg agaatttcaa
     6661 tcaccgcatc ccactgatca ttagtgagat cggcacccgt tgggttgtgg caacatggat
     6721 gcagcaacac aatactgcgg gcaggtaatg ttttcagcgt cgccaacagg tcattaaagc
     6781 gcacgctgtt agtcacttcg tcataccagg ggtaagtact cacttcgaat ccagccccgg
     6841 cgaatattgc tacgtggttt tcccaagtag gatcgctgac ccagacgcct gattccggga
     6901 agtagcgttt caggaaatcc gcgcccactt tcaatgcccc tgagccgcca agggtttgaa
     6961 tggttgctac gcgctgttgt tgcagtaccg gatggtccgc accaaacagc agcggcgcaa
     7021 tggcatggcg atagctgtta agcccttcca tcggtaaata aagcgaagcg ccatggggct
     7081 gcgcattcag gcgcgcttcc gcctccgcca cggctttcaa ttgtggaata attccgtctt
     7141 cgttgtagta cagaccgata cttaaattca ctttgtcgct gcgagggtct tctttaaaac
     7201 gctccataag cgtaagaatc gggtcgccag cgtaggcgtc aactttttga aacacgcgat
     7261 ggttctccag gtttacgggc aggtggttaa aacacaataa accggaagaa ggcgaagatc
     7321 gagtggatgt tcaggagcga acggcaatta gcaacagagt gagactaatg ataaacgtac
     7381 atccgccaga ggcacgacct ttataagagc atgaaagaac atcaacttat tgaattttta
     7441 ggattttatt ggccggataa ggcattcacg ccgcatccgg cacagacaat caaatattac
     7501 agaacgatta atccacgtat ttcatcgcga cccttgaagt caggcgcgta ataagttcgt
     7561 aagcgcttac tttcgtcatt tcagcgatac gttctacggg caaaccttcg ccccataaaa
     7621 tgaccggatc cccggctttg tcctgcgcct gtggacctaa gtctacgcag atcatatcca
     7681 tcgccacgcg cccgacaatc ggtacttcgc gaccgttcac cagcactggc gtaccggacg
     7741 gcgcggcgcg cggataacca tcgccatagc ccatcgcgac tacgccaaga cgggtatcac
     7801 gttcgcttac ccaggttcca ccataaccaa caggctctcc ggctttatgc tcacgcacgg
     7861 caatcaggct ggaggttagt gacatcactg gctgacagcc aaaatcggca ccggtggagc
     7921 gatcttccag cggcgagacg ccataaagaa tgatgcccgg gcgcacccag tcaaaatgcg
     7981 actgtggcca cagcagaatg ccacccgacg cggcaatgga acgttgacca ggtttgcctt
     8041 cgcaaaaggt attaaagata gcgagttgtt tctcggttgc gccacatttt ggttcatccg
     8101 cgcgcgcaaa atggctgacg atattcaccg gctgacgaac gtttttgcac tgggtcaggc
     8161 gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata ccggtatcga
     8221 gtttcatcca gacggtaacc ggctcgtcca agctagcctc ttccagcgca gccagctgtt
     8281 cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga tctctggcat
     8341 caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc agtcgcagag
     8401 cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa
     8461 gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg gcaggtgcca
     8521 gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg
     8581 cttgcatttg tgttccttga taattgtttg ctttaattac ctaattcata aaataattat
     8641 tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg accgttaaag
     8701 gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat ttccgcgatg
     8761 ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat gatcaagtcc
     8821 gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccggacg tttgtcggca
     8881 cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt cagttctttc
     8941 gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt atcggaaagc
     9001 gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc gccgtgttca
     9061 cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga agagtcatcg
     9121 atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg cgcccagtct
     9181 tcatcatcga gctgcccggt acggatttta gtctggtcaa cgcgcgacag cgacgccaga
     9241 gaacgcatca tgatctgttc tgatggcatc tccagcgaga agataagtac tggtttatcc
     9301 tgcaacattg ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc catcgacgga
     9361 cgcgcggcga cgatgatcaa atccgacggc tgcaagccag cggttttttt gttgagatcg
     9421 tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct gctgaaacaa ctgctcaata
     9481 cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc tttgtttgca
     9541 cgactttcgg caattttaaa gacgcgggat tcagcaaggt ccagcagatc ttcgctggta
     9601 cgcccctgcg gatcaaaacc ggcttcagca atctcattcg caaccgagat catctcacgg
     9661 acaacggcac gttcacgcac gatgtccgca taagcactga tgttcgccgc acttggcgta
     9721 ttttttgaca gctctgccag ataagcaaaa ccaccgacgc tatcgagttg cccctggcgt
     9781 tccaacgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg caaacgcgcc
     9841 atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat cgtctgctac cacacgctcg
     9901 gctacatcat cccagcgttc gttatccagc attaaaccgc ccaacaccga ctgctccgct
     9961 tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc gcggggttca
    10021 gcctgctgtt tgttgaaggg tttatttcct gccatagtga atggagttac cgaaataaag
    10081 aatgggtcga aactttacca tatgaagcag accctgacga tacgttctgg aggacacatg
    10141 gcaacacgaa ttgaatttca caagcacggt ggcccggaag tacttcaagc cgtagagttc
    10201 actcctgccg atccggcgga gaatgaaatc caggtcgaaa ataaagccat cggcatcaat
    10261 tttatcgaca cgtatatccg cagcggcctt tacccgccgc catcgctacc cagcggatta
    10321 ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca tattaaggct
    10381 ggcgatcatg tagtctatgc gcagtcggcg ttaggcgctt acagctctgt gcataacatt
    10441 aatgcggata aagcggcgat tctgcctgcg gcaatttctt ttgagcaagc tgcggcatcc
    10501 ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa cctatgaaat taaacccgat
    10561 gaacagttcc tgttccacgc agcggctggc ggcattggct taattgcctg ccagtgggca
    10621 aaagccctgg gcgcgaaact catcggcacc gtaggaaccg cgcaaaaagc gcagagcgcg
    10681 ctaaaagccg gcgcgtggca ggttattaac tatcgtgaag agaatctggt cgagcgattg
    10741 aaagagatca ccggcggtaa gaaagtgcgc gtagtgtacg attccgtggg cagagacacc
    10801 tgggaacggt cgctggattg cctgcaacgc cgcggcttaa tggtcagttt tggcaactca
    10861 tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc aaaaaggctc gttgtatgtg
    10921 acacgccctt ccctgcaagg ctatatcacc acgcgggagg aattaaccga ggccagtaat
    10981 gaactgttct ctttgattgc cagcggtgtg attaaggtcg atgtcgccga gcagcagaaa
    11041 tatccgctga aggatgcgca gcgtgcgcat gagattctgg aaagccgggc gacgcaaggt
    11101 tccagcctgc tgattccata aaagaaatag ggcttccacc tgggaagccc tttcttttta
    11161 tagttcggct gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa tagtgacaga
    11221 tttgattatc aattcctatt ttgttctaag gataaaacct taggttgtga tcatccgcac
    11281 aatcccttag taacgccagc ggtcataacg ctgatatttc ggcatttttg gtgctttaat
    11341 cgccttaata acccacacca ccgcaatcgc cagtagtaac cacggcagca acttaatcat
    11401 caatgccagc ataccgccga ggaacataat ggccgtcgcc acaaccagcg cggcgataat
    11461 gcccagcaac gaaacgccgg tgaccatcag catgacaaaa aagccaatca caaaaagtag
    11521 ttccagcatg atgctctccc aaatatgaaa tctcttgctg gcattacaag aatcatgcca
    11581 aaaataatct attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg cgtggtgaat
    11641 ttgactactt tttggtgaaa agttaacgct tatccgccac cagtttgagc gcgtgttcca
    11701 gcacgttaat gtcagcaccc gctttatggg cattttcact taaataacgc cgccactgcc
    11761 gcgcgccagg aataccctgg aacaaaccca gcatatgccg gataatatgg ccgagatacg
    11821 tcccctggct gagttcatgc tcaatgtacg gatacatggc acgcactact gccaccggat
    11881 cggcatcggt atccgaggaa ccaaagatct cccggtctac cgccgccaga atacccggat
    11941 tctgatacgc ctcgcgcccg accatcacgc catccatatg ttgcaaatgc gctttagctt
    12001 cttccagcga cttgatacca ccgttaatcg acattgtcag atgcgggaag tcacgcttca
    12061 gttgatacac acgcggataa tcgagcggcg ggatttcacg gttttctttc gggcttaacc
    12121 ccgaaagcca ggctttacgt gcgtggatga tgaacatctc acactcgcct ttgccggaaa
    12181 cggtattgat gaaatcgcag agaaattcat agctgtcctg gtcgtcgatg ccaatacgcg
    12241 ttttcaccgt caccggaatc gacaccacat cgcgcatcgc tttcacgcag tcggcaacca
    12301 gctgcgcatt acccatcaga cacgcaccaa acatgccgtt ctgcacccgg tcagacgggc
    12361 agccgacatt caggttgatc tcatcatatc cgcgcgcttc tgccagcttt gcacactgcg
    12421 ccagcgccgc cggatcgcta ccgcccagtt gcaacgctac cggatgttct tcttcactgt
    12481 acgccagata atcaccttta ccgtgaatta tcgcccctgt ggtcaccatt tcggtataaa
    12541 gcaacgtatt gcgggaaagc agacgcaaga aatagcggca atgtctgtcc gtccagtcga
    12601 gcattggtgc aacactaaac cgaccactcc agtgaacgtc agttttttca ggcattgcgc
    12661 tggtttggtt gattttttgc atttcagaat taccgtgcat tttcaaatgt agagattatt
    12721 ttatcgatat atcatggggt tatgttattc agcttcactg ttcaggaggc tcaatagcgg
    12781 ggtactatac cataacaaca ggaagcgcct gtctcattgc aaaagaaaat tgagatcctc
    12841 tcaaggcatg aagctctcac gaagtgatgg aaataatctt attagccgtt aactttgtta
    12901 aggccaatga taaacaatcc aggttcgacg ataaataaaa aatcacacat taaactctgg
    12961 tgatatatct ccctgctaat agcattgata gagaaaaaag aacccaataa atattgggtc
    13021 cttttatata atgccctcca tactatcgaa gaacttcaca tattatttct ccgatttaac
    13081 cccgaacaag tcataaatta atttagaagt gtctgtaagt attttaatct cttcctttga
    13141 ggttgggtca aacgacttcg cgaagctaat taattgtggt gcagcatctc gcattttgct
    13201 taaaatatca ggatcgagcg ttccttcatt caccagatgt gacatcttat ccagatagcc
    13261 atcaaaattc atttctcccc catctgccag acgcttcatc tctcccaggt acttcttcat
    13321 atcacgttga tataattttt caaactgagc tttcaggtag ctctcattat tttcattaat
    13381 atacactgtt tctgataaat ctgcgtaggc actagaatat gaaagtgaaa ttaacagcga
    13441 agcaaataat tttaagctgt tgtcatcatg ttcctggcag gcattaacaa acgttaaaaa
    13501 accagaacct attgcttgaa aatgtatatt cgctaaaggt tcattatcct tagattcctc
    13561 ataaaatagc tgaaccgtgg agggaaggcc attcttagtt tctacatcaa aagtacattg
    13621 ttcaatgggc attgcatctt tattatcccc aggattacat atactattaa tcgcgaaatg
    13681 aaaaatagcc cggtcaacaa ctgatgcaat aaagtcacga tcgtttaaat cctcatcagt
    13741 gcaatcgtca gtatacttat tgacccacat tttgagatca tttatgtgct caattaaaaa
    13801 cacatttttt tacgtgcatg ccataaatta tgcgtgattc gttgaataaa caaatctatt
    13861 tttgcaataa ttatatcccg atgacgcagc gctgcataat caatagattt accatatata
    13921 ttatttaatt tacctttgat tgaacctcta ttatccaatt tatctttggt tgcatcttta
    13981 ttttcgaatt gatttgaatt gagtgtttta ttaaagtatc cagttgcacc gggtattatc
    14041 ttcaacatga ttcatctacc ttttttgtcg taccgtgtcc tccttataat tcttttgcca
    14101 tctggtcact atcaaaaatc gctcataatt tctgaactac aggtatgcgt ttaaacccag
    14161 ttaaataaat agcgagttaa cttgaagcaa tttatttaaa aatgaattat taattattat
    14221 cgtgttgata atggtacgat aaggcaacac aacaccttac ctacaaaaca gaaaatcaac
    14281 attacggcaa caataagggt tctcggtgtt tttcctggaa catggtaaag taaggacatt
    14341 cttaaccccc actttgaggt gcccgatgga aaagaccaca acgcaggagt tattagcgca
    14401 ggctgaaaaa atctgcgcgc agcgtaatgt gcgcctgacc ccacagcgcc tggaagtgtt
    14461 gcgcctgatg agtctgcagg atggcgctat cagcgcttat gatctgcttg atttactgcg
    14521 cgaagctgaa ccgcaagcca agccgccaac ggtttatcgc gcgctggatt ttctgcttga
    14581 gcaaggtttt gtgcataagg tggaatccac caacagttat gtgctctgtc atctgttcga
    14641 ccagcccacg catacgtcag ccatgtttat ttgcgatcgc tgcggcgcgg tgaaagaaga
    14701 gtgtgcagaa ggcgtggaag acattatgca tacgctggcg gcaaaaatgg ggtttgccct
    14761 gcggcataat gtgattgaag cacatgggct ctgtgcggca tgcgtggaag tggaagcgtg
    14821 tcgtcatcct gaacagtgcc agcatgatca ctctgtgcag gtgaaaaaga aaccgcgtta
    14881 agagggcgta catccttgta cacgtcgggc aggagggatt aattaccagc gatattcatt
    14941 gcgggtttcc caatccacga cctctttttc tgcctgatct ttctgataac cataacgttc
    15001 ctggatttta ccgaccagtt gatcacgttt accttcaatg atcgtcatat catcatcggt
    15061 cagtttgccc cattgctctt tcactttacc tttaaactgt ttccagttac cgccggcttc
    15121 atctttattc ataatcaaga cctcatcgtt aggttgtgaa tgagagtacg ttcacttttc
    15181 ttctgaacgt gagattaagt gtagtcagca atttggctta ggataattta ttcagaattt
    15241 ttaaccgtca cgttgcggca aaccaggtac cgttgcgcca gtgacgccgc cagatagccg
    15301 ccagagaaag cccgcgcaac gccagaaaga cggttaatgc cagccacaaa ccatgattac
    15361 ccagccacgg cagcgtaagg agcgtcagcg caaaacctgc ggcggccacc gccatactgt
    15421 tacgcatttc ggcggcacgc gttgcgccta taaacatgcc gtccagcaga taacaccaga
    15481 cgccaaccaa cggcaaaatc acctgccaga taagatagcg gtcagccagc tgctgaatct
    15541 gggttaacga cgtcagcaac gcaatgatgt gttcccctgc cagcaaataa accaccgaaa
    15601 atagtaacgc tacaatcccc gactggcggc acgctgcccg ccatacatcc agcaactggc
    15661 taccgtcgcg cgcaccgtat gcctgaccgg agtgcgcttc aaccgcgtag gcaaaaccat
    15721 caagcgcata ggcggtaaag gtgagtagcg tcatcagaac cgcgttaaca gcgataatgt
    15781 cactccccag tcgcgcacca agtacggtga tcgcgccgaa acagagttgc aacaacagcg
    15841 aacgcagcat gatatcgcgg ttaagcgcca gcaagcgacg gaagtttcct cgccaggcag
    15901 ttttcagcat ttcgccggaa attccgcgta gtttgaggat tttacgcacc attagcagac
    15961 caatcagcaa tgttgcatat tccgcaataa ccgtcgccag cgccgcgccc tgcacgttca
    16021 tatgcagccc catcaccagc cagacatcca gcacaatgtt gaggatattg ccgaccacta
    16081 acaaaattac tggcgcacgg gcatattgca cgccgagtaa ccaaccgagt aataccagat
    16141 tcgccagcga cgccggagcg cttaaccagc ggatttcaag aaagcgccgc gcctgttcca
    16201 ggaccgcttc actaccgcca acaatatgca gcgccagatc gataatcggc gtacgcaaca
    16261 gcgcaattaa cgccccggcc cccaacgcca acagcaacgg ttgcaccagc gcacgggcta
    16321 atgcctgagg atttttggca ccataagcct gcgcagtcag cccggtggtg ctcatgcgta
    16381 aaaacagcaa cagcataaag agaaagctgg tcgccgttgc accaaccgcc acgccgccca
    16441 aataaaccgg actatcaaga tgaccaatta ccgccgtatc gaccagtccc agcaacggaa
    16501 cggtgatatt ggagaaaatc atgggtaagg cgagatgcca gagtgcttta tcagatgaag
    16561 tgaggaatgc catgcagaca agcctgatga agagagatga aaaacaaacc gcgacgccaa
    16621 gcggcatcgc ggtctcagag atatgttaca gccagtcgcc gttgcgaata accccaaccg
    16681 ccagcccttc aatggtgaag ctctgctgac gaaggtcaac gacaattggt ttaaactcgc
    16741 tattttctgg caacagttcg actttattgc cctgtttttt caggcgctta acggtaactt
    16801 cgtcatcaat acgtgcgaca acgacctgac cgttacgtac atcctgagtt ttatgcactg
    16861 ccagcaagtc gccatccata atgccgatat ctttcatcga catcccgctg acgcgcagca
    16921 ggaaatcagc attcggcttg aacaaggaag gatcgacctg ataatgacct tcaatatgct
    16981 gttgcgccag aagcggttca ccggcagcca cacgacctac cagcggcaac ccttcttcct
    17041 cttcctgcaa cagacgaatc ccgcgtgatg cgccggaaac aatttcaata acgcctttgc
    17101 gtgccagcgc cttcagatgt tcttcagccg cgtttgggga acggaacccc aaacgctgcg
    17161 cgatttccgc acgcgtcggc ggcatacctg tctggctgat gtgatcacgg atgagatcaa
    17221 acacctcttg ttgcctggcc gttaacgctt tcattccgcc ccctgggtgt atatacagtt
    17281 atactgtgag tatatacagc aaaaggcgat tttggaacca taaactgcac aataaaccag
    17341 agatttatcg aaatctggaa gggttatcca aaatgcgacc ataacaggat gcaccaggta
    17401 atcacggcga cgataatggc aatcagcacc gcggcggatc ccatatcttt tgcgcgtccg
    17461 gaaagctcat ggtattcaga gccaattcgg tcaaccactg cttcgatggc gctattgagg
    17521 atttccacaa tcatcaccag catcacggag ctgataagca gcacgcgggt aattgcgtcc
    17581 acatccagcc agcaggcgat gaccaccgcc aacaataccg ctacgccttc ctgacggaat
    17641 gccgcttcgt tgatccatgc agcgcgtaaa cctttccagg aatagccagc agctttgata
    17701 attcgggtga atccagttgt attattggcc attgaaagaa cctttttaca ttatgagcgt
    17761 caatatcagt gtaccgttaa atccgatgat tcgggtttgc gtaacgccgc gcgaaacatg
    17821 agcggatacc acagaatttc ccatgacttt ctgctatcct tgccgcgcat ttgcattatt
    17881 aaccagaggc tttacatcgt ttatgtccgg ctggccacga atttactaca aattactgaa
    17941 tttaccatta agcatcctgg taaaaagcaa gtctattccg gcagatcctg ccccggaact
    18001 ggggctggat acctctcgtc caattatgta cgttttaccg tacaactcga aagcagattt
    18061 gctgacgttg cgcgcccagt gtctggcaca tgatttgcct gacccgttag agccgctgga
    18121 aatcgacggc acgctactgc cgcgctatgt gttcattcac ggcgggccgc gtgtattcac
    18181 ctattacacg ccgaaagaag agtctattaa gcttttccac gactatctcg atttgcaccg
    18241 tagcaaccca aatctggatg tgcagatggt gccagtatcg gtgatgtttg gtcgcgcgcc
    18301 ggggcgtgaa aaaggcgaag tgaacccgcc gctgcgtatg cttaacggcg tacagaaatt
    18361 tttcgctgta ctgtggctcg gtcgcgacag ttttgtgcgt ttctcgccgt cagtgtcgct
    18421 gcgccgtatg gcggatgaac acggcacgga taaaactatc gctcagaaac tggcgcgcgt
    18481 ggcgcgtatg cactttgccc gtcaacgtct ggctgccgta ggcccacgtc tgcctgctcg
    18541 tcaggatctg tttaataagc tgctcgcctc ccgcgccatt gccaaagcgg tagaagatga
    18601 agcgcgcagc aaaaaaatct cccatgaaaa agcgcagcag aacgcgattg cgctgatgga
    18661 agagattgcg gcgaatttct cttacgagat gatccgcctg actgaccgca ttctgggctt
    18721 cacctggaac cgattttatc agggcatcaa cgtccataac gccgagcgcg ttcgccagct
    18781 ggcccacgac ggtcatgagc tggtgtatgt gccttgccac cgcagtcaca tggactacct
    18841 gctactttct tacgtgctgt accaccaggg actggtgccg ccgcatatcg ccgccgggat
    18901 caacctgaac ttctggccgg ccgggccgat tttccgccgt ctgggggcgt tctttattcg
    18961 ccgtactttt aaaggcaata aactttattc caccgttttc cgtgagtatc tcggcgaact
    19021 gttcagccgt ggttattccg tcgagtactt cgtggaaggc ggtcgttccc gcacggggcg
    19081 tttgctggat ccgaaaaccg gtacgctgtc gatgaccatt caggcgatgc tgcgtggcgg
    19141 cacgcgtccg attacgctga ttccgatcta tatcggttat gagcacgtca tggaagtggg
    19201 tacttacgcc aaagaactgc gcggcgcgac gaaagagaaa gagagcctgc cgcagatgct
    19261 gcgcggttta agcaagctgc gtaatctcgg tcagggttac gtcaacttcg gtgaaccaat
    19321 gccgttgatg acctacctta accagcacgt accagactgg cgtgaatcta tcgatcccat
    19381 cgaagcggtg cgtccggcct ggttaacgcc gacggtcaat aatattgctg ccgatctgat
    19441 ggtacgcatt aacaacgcag gcgcggcaaa cgccatgaac ctgtgctgta ctgcgctact
    19501 ggcatcacgt cagcgctcac tcacccgcga gcagttaacc gagcaactca actgctacct
    19561 ggatctgatg cgcaacgtac cttactccac ggactctacc gttccttcag ccagcgccag
    19621 cgagcttatc gatcacgcgc tgcaaatgaa caagtttgaa gtcgagaaag acactatcgg
    19681 cgacatcatc attctgccac gcgagcaagc ggtgctgatg acctactatc gcaacaacat
    19741 tgcgcatatg ttggtgctgc cttcgctgat ggcggcaatt gtcacccagc atcgccacat
    19801 ctcccgcgac gtattgatgg agcacgtcaa tgtgctttac ccaatgctga aagcggagct
    19861 gttcctgcgc tgggatcgcg acgagttgcc ggacgttatt gatgcgctgg caaatgagat
    19921 gcaacgtcag gggctgatta ccctgcaaga tgatgagttg catatcaacc cggcgcattc
    19981 tcgcacccta cagctgctgg ccgcaggcgc gcgcgaaacg ctgcaacgtt atgccatcac
    20041 cttctggttg ttgagtgcca acccgtcgat caaccgcggt acgctggaga aagagagccg
    20101 caccgttgcg caacgtctct ccgtgctgca cggcatcaac gcgccggagt tcttcgacaa
    20161 ggcggtgttc agttctctgg tgctgacgct gcgtgatgaa gggtatatca gcgatagcgg
    20221 cgatgccgaa ccagcagaaa cgatgaaggt ttatcagttg ctggcggagt tgattacatc
    20281 agacgtgcgt ttgacgattg agagtgcgac gcagggcgaa gggtaatcag agagaattgc
    20341 cggatgcggc gaaaacgcct tatccggcct accatgacct gcaaattcaa taaattgcga
    20401 ttcaccgggt aggcctgaga agcgcaacgc atcaggcaac ccagaagaaa gccggatgat
    20461 catccggctt ttttacatca tcagaaatgc cagtaactca ttgccagtcc taaaaacagt
    20521 accagaccaa catagttatt attcataaat gctttaaagc aggcttcacg ctcgcggttg
    20581 gcaatcagtt tttgttgata aacaaacagc gcgccagcca ccacaattga ccagtaatat
    20641 ccccagccta agccatttaa ctcaccgatg atcgccatca gtgccagtac gccaatctgc
    20701 aaaataccaa taatcaattt atcgtattgg ccgaacagga ttgccgtgga tttaatgcca
    20761 atcttcacat catcatcgcg gtcaaccatc gcatactgcg tgtcgtaagc caccgcccag
    20821 agaatattgg cgaggaacat taaccagcaa ctcaatggca ccgactcact cacagcggca
    20881 aaagccattg gaatcgacca gccaaacgcc gcgcccagca ccacttgcgg gagatgggta
    20941 taccgcttca taaacgggta cacccacgcc aatgccagag cggcaatcga caacagaatg
    21001 gtcatcgtat tcagcgtcag aaccagtaaa aacgaaatca gtaccagcac tacaaacagc
    21061 gcgcgcgcct ctttctctgt taccgcgccg ctgggaagtg gtcggttcgc cgtgcgctta
    21121 acatgaccat caaacttgcg atcagcataa tcattcacca cgcaaccagc agcgcgcatc
    21181 agccagacac ccgcgacaaa caccgccatg atccagagct gaggaacgcc cggtgtcgcc
    21241 acccacaacg cccataatgt tggccagagc agcagtaacg cgccaattgg cttatccgta
    21301 cgcattaagc gatgaaacgc cagcagctta ttctgcgtca gactccactc catatttttt
    21361 tcctcttagt acaacggtga cgccggtaaa aacagttctg ttagcaacag cggtttaccg
    21421 cttaatcgca ggcgggaacg tcgcccccac agtccggcat cacggcctat ctcgataaaa
    21481 tcccgggtta gtgtcgatga tgtgaacaga tagcgtccta acggcgtttt acccaatttt
    21541 tgtaacgcta gttccggccc gcttaatgtt gatacaggaa cgacggtacg accggcaagc
    21601 cacggttcac catcggcaca taacaaaatt tcacgtaacc agtaacgaga ctctttcggc
    21661 agcagcggca gttcttcggg gatttcattc tgctcgacaa acccctcgcg gatcatcgtc
    21721 acactcaccg tttttccctg ctgttcaaaa cgttttgtca tggaatcctc cagcaacagc
    21781 cagtcgagca gttgcggatc cagggcaggg atctctttaa aatagcgcag cgcacgcagt
    21841 tgcgttaacg cggggtgtga catgccgaac tctccgttac ataacgtaaa ggcattgtat
    21901 cgcagaaaag tgtttacggg agatgtaaag caaattaagt gatctaatgc gcaacaaaaa
    21961 cgcaacaatc gcatgaagtg gtaaaacgct gttgaacctt acgctttgcc gatgcccgtg
    22021 ctggcgaggg caaccatagg ggcatcaccg cccatgatgc taggaacggt gatgttttcc
    22081 tttacttaaa tacctttatt ctttatacga gtagctcgcc aactcgcagt aaaaattggg
    22141 aaaagcgcat atttaccatg agatttggag ctgaaaagtg tttcctgagt aagattaata
    22201 ggcaacatct tatgtctgga tttatagata cacgtggaat tccaattggt atcttttgta
    22261 ggattcaaca tatcatgtaa gcgctgggag cttaccacca taacaggccc atcatcgaca
    22321 gaggagaaca ggataaatat attatcgttt cgtgttaatt tattagtgtt aaaggttctt
    22381 aacttttctt cagtttctat aaatataggt gaaacatcat cactacgaat ccactcttta
    22441 taaagatacc aggcatttgc tttgatcata tcatccgttg atcgtaaggc ctgatcgata
    22501 agttggataa aaaaattgtt atttttatca accagccctt tattcatatt aaatctgtta
    22561 ataaaatgga tcatctgttg ggtatcgaaa ttcaccttac tgaatcgatg ttgacgcata
    22621 aaattatcga acttgtcatc ttccccagaa tgtatataac tacgtataat tggatctttt
    22681 atataaggac aatggctaaa tgttttcttc atattctcaa taaagttttt attaacaata
    22741 ttgagaattt ttaattgcat caccagtttt actgccagaa cggatttcaa tatcattgca
    22801 ttatctggca atgtatcaat catttttata atgttattga tataaccatc gagatcttca
    22861 ttcccaaata tatctgaata atcaatagtt aattccaatt gttctgtaat gcaatcttaa
    22921 aaattccctg tcgacattcg cacatgatca aacataacag ctgtcaaatt cgatttaata
    22981 aatgatattt ctttggcaga cagaaactct gctctggtga agtgcgaagc gtaaagataa
    23041 caattatcga agtttgaatt tctaatctga cattcgcgaa aattactatg accaagttga
    23101 ctctgcgaga agtcaactgt atcaagaata ttttggctaa aatcaacaca ttcgccactc
    23161 gttttgagaa aaatcacatt gtgtaaacgt cgacatttaa atatgacatt atcaactgtt
    23221 gccttagtaa aattgcaatt ctcaatttct ttacattcga aagttacatg ttgtagattt
    23281 gatgataaaa aagacacctt agaaataacg tcacctttaa aatgacaatt tgttaaatca
    23341 aaatcagaca ggtcgatata cccctgcgca tctttatcca tcagaaaatt acaagcgcat
    23401 tctatttttt catttctgta gaattttaca cttatagtat cattactaat tgagacttca
    23461 cgtaacatat ttattgcatg atttttttcg ttccgttgtt ttatatttaa aaatatatca
    23521 ctgatataaa taaagtaatc tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa
    23581 tcaagatcta ctatattatt tccttgcacc acatcatgtt tcatcaaaga gttattgccg
    23641 ccagaatttg tgatatgacc tatcataaaa cctctgtaat acagaaataa aataacctgg
    23701 aaaagtagcc tgagcgtcca gacactaccg gcgaaaggaa aagaatctct gataatgcat
    23761 tgaaataatg gacattctta gtgaagaaga agtgaagtac tataaaaaat cgctcatatc
    23821 ccaggaagga agcataaaaa aaggtgcgcc aggagacgca ccagttgttg caaaatcagc
    23881 actgtggggc gtaattaccc cttgcctttt acactgctga taaaggtgga acgggcagat
    23941 gtcgatccca ggcgttcagc ttcatcaagc agttttaacg ccttatcaac atcacctttc
    24001 gcgacagcgt ttttgatcgc ggtattaaaa taactttccg tgtcgttgag catcggctcg
    24061 cttttcttca ccggcaccgg agcaggtgca gccacagctg gtgccgccgt gttgcctacc
    24121 gtaaccggag ctggagcgga agaaccaaat agcggtccta ccaacacgct ggagctggag
    24181 ttcgttttga ctttcagttt cagtaagcca tcgctggtat gacgagcaac cggatcggga
    24241 atatccggga tcgagttacc gacgcctttg gcataggctt tagccgggtc gagcagttgg
    24301 gtcgtctgct ggagatcttt ttccgtggta aagaccagaa cataaagttt ttgctgcccc
    24361 aacgccggtg tcaggcgcat aacgccttcc agccgatctg cactcatcac gcctagttcc
    24421 tggtaggtga aataactgct ggggaagaag gctgatgggg tcatgttctg atcaagaatc
    24481 agcacgttcg gcgcaaaaac gctggtttgt ttgttcactt cgctggtcag cgtcagggtc
    24541 agttcgccaa tgtttgccgg gacgctgtac gcagcaaccg gaccactgat gccgggaacg
    24601 ttcagttgtt ggccgccggt cgccagttgg gtggtctggg ttttagattg atcgaccggt
    24661 gtccaggtga gttgttgcag cgcggcagat ggaatggctg gcgcgtcgct ggtgttttgc
    24721 ggtacgtagt taacatcggc aaggctaatt ccaggcgcgc ttgccagtaa ccctgctgat
    24781 aaacagaggg cgatgagact tttattcatt ttcattgttt tcacctcaaa atctggagct
    24841 cagcggtagc caggcaatag cgcgctaaac ccgataatca gaggggcttt cgcccctttc
    24901 agataatgac aacctgtttt tatgccggat gcggcgtaaa cgccttatcc ggcccaggtt
    24961 ttgctattac caccagattt ccatctgggc accgaaggtc cactcgtcgc tgtcgccacg
    25021 accgaagctg ccgccgttga aatcagcagg aacggctttg ccgtagttcg ggttaacctt
    25081 gctatcgccg ttgtagtcgt aaccccattt ctcatcccac ttggcgtagg ttgcgaagac
    25141 acgaatagcc gggcgtgacc agatgctgtc gccagcctgc cattgttgtg cgagggtaat
    25201 tttgtactga ttgttcttgt cgccggtgcg ctgggattcg acgttgtcgt agccgatttc
    25261 catcacggtg ctcatgattg gcgtccactt gtacatcggg cgaataccaa cggtccacca
    25321 cttggtgccg ttgtcgttat cccagttgat atcctggtac atacccacgt acatcatgtc
    25381 ccagttgtcg cccatggaga tcgcaccgtg gtcgaggata cgcagcatgt gaccgttgtt
    25441 gttgatattg taggcaaatt tttcgttatc aaacgcgacg ccagaaccct gcgacagacc
    25501 tttaccctgc gaggtcatcg agtcagtagc gtactgaaca acaaacttgt taaagccctt
    25561 caggacactc tgagtatgtt cagcagtgaa taaccagccg tctttcgatg cgccatcaac
    25621 cagacgatag ttatcacgca ggttggcacg accgtagtcg acacccagtt ctaatgtgcc
    25681 gcccgggttg atttccatct gcgctaaacg cacatcgaaa acgtcgttcg cggtttcgtt
    25741 ggtatagtca taaatattgt tgctggcaaa agaggaagaa ccaccagctt cagaggagcg
    25801 ggttgctgcc agagagagtt taccgaagcc aacatcgatg ttttccagac cggcaccagg
    25861 accagaaata tcccagtagt agaagtcgat catatgaacg tcatgacgtt ggtagaagcg
    25921 cttacctgcc cagatggtgg aacctggcag ccattcgatc aggttttttc cctgcacgtt
    25981 tgcttcacgg aaggccggat cggtagcttc ccagtcattc tgttgcgcga cggaataggc
    26041 cacgttagtg tcgaaataga agctcttatc gccctctttc cacacttcct gacccaattt
    26101 taattcagca taagtttcac attcgttgcc aagacggtat ttactttgag caccggtagt
    26161 ctggaaacac tgttgttcac cgccgctacc tgtccagcca ataccggaac gtgcatagcc
    26221 gtggaaatca acagccattg cctgagcaga cattacgccc gctgcgacgg caaccgccag
    26281 aggaagtttg cgcagagtaa tcatcattct atctcctgag tcattgcttt tcttttttca
    26341 catcacctgt gacaggcttt gtgtgttttg tggggtgctt aaacgcccgg ctccttatgc
    26401 agtcgacgac atgcagtgcc atcctcacgg aacagatggc aacgctctgg cggcaggccg
    26461 atagcgaatg tggcaccttc ttctaccaac accacgtcgt tctggcggta caccaggttt
    26521 tgacgaatgg aagggatctg gatatagatt tgggtttcgt tgccgagttg ctcgacgacc
    26581 tgaacttcac cctcaaggat gacgtcagcg atatcactcg gcagtagatg ttccgggcga
    26641 atacccagcg acatattggc tccaacctgg acatcacggc tttcaacagg cagccagact
    26701 tgttgacgat ttggcatcgg cagctccacc tgcacttgat cgattgcggt ggcggtcact
    26761 tttaccggca ggaagttcat ctttggcgaa ccgataaatc cggcgacaaa acggtctgcc
    26821 ggatagtggt acagttccag cggtttccca acctgtgcca cgcgaccggc gtccagcacc
    26881 acgattttgt cggccagcgt catcgcttcg acctgatcgt gggtgacgta aatcattgtg
    26941 cggcccaggc gtttatgcag acgggagatt tcgatacgca tttgcacacg cagtgcagca
    27001 tcgaggttgg agagcggttc atcgagcaaa aatacgcttg gctcggccac cagcgtacgg
    27061 ccaatcgcca cgcgctgacg ctgaccaccg gagagcgctt tcggtttgcg atccagcaaa
    27121 tgcgccagtt gcagcacttc cgccacctgg ttgacgcgtt ggttaatcac ctcttttttt
    27181 gcgccagcca gtttcaggcc aaatgacatg ttttctgcta ctgacaggtg gggatagagc
    27241 gcgtaagact gaaacaccat accaacgccg cgttctgctg gcggagtgtc attcatccgt
    27301 ttctcaccga tgaacaggtc gccgctggtg atcgtctcaa gcccggcaat catgcgcagt
    27361 aaagtcgatt taccgcagcc agacggtccg acaaacacca cgaattcacc ttcatggata
    27421 tcgagattga tatctttcga taccacgacc tcgccccagg ctttcgttac attttgcagc
    27481 tgtacgctcg ccatgccctt ctccctttgt aacaacctgt catcgacagc aacattcatg
    27541 atgggctgac tatgcgtcat caggagatgg cttaaatcct ccaccccctg gcttttttat
    27601 gggggaggag gcgggaggat gagaacgcgg cttctgtgaa ctaaaccgag gtcatgtaag
    27661 gaatttcgtg atgttgcttg caaaaatcgt ggcgatttta tgtgcgcatc cccacattac
    27721 cgccaattct gtaacagaga tcacacaaag caacggtggg gcgtaggggc aaggaggatg
    27781 gaaagaggtt gccgtataaa gaaactagag tccgtttagg tgttttcacg agcacttcac
    27841 caacaaggac catagattat gaaaataaaa acaggtgcac gcatcctcgc attatccgca
    27901 ttaacgacga tgatgttttc cgcctcggct ctcgccaaaa tcgaagaagg taaactggta
    27961 atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag
    28021 aaagataccg gaattaaagt caccgttgag catccggata aactggaaga gaaattccct
    28081 caggttgcgg caactggcga tggccctgac attatcttct gggcgcacga ccgctttggt
    28141 ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag
    28201 ctgtatccgt ttacatggga tgccgtacgt tacaacggca agctgattgc ttacccaatc
    28261 gctgttgaag cgttatcgct gatttataac aaagacctgc tgccgaaccc gccaaaaacc
    28321 tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg
    28381 ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg
    28441 ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg
    28501 aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc
    28561 gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc
    28621 ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg
    28681 accttcaagg gtcaaccatc taaaccgttc gttggcgtgc tgagcaccgg tattaacgcc
    28741 gccagtccga acaaagagct ggcgaaagag ttcctcgaaa actatctgct gactgatgaa
    28801 ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag
    28861 gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa
    28921 atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtacgtac tgcggtgatc
    28981 aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactcgtatc
    29041 accaagtaat gctgtgaaat gccggatgcg gcgtgaacgc cttgtccggc ctacaaaact
    29101 gaaacgcatg taggcctgat aagacgcatc agtgtcgcat caggcagttg ttgtcggata
    29161 aggcgtgaaa gccttatccg tcctggaatg aggaagaacc ccatggatgt cattaaaaag
    29221 aaacattggt ggcaaagcga cgcgctgaaa tggtcagtgc taggtctgct cggcctgctg
    29281 gtgggttacc ttgttgtttt aatgtacgca caaggggaat acctgttcgc cattaccacg
    29341 ctgatattga gttcagcggg gctgtatatt ttcgccaatc gtaaagccta cgcctggcgc
    29401 tatgtttacc cgggaatggc tggaatggga ttattcgtcc tcttccctct ggtctgcacc
    29461 atcgccattg ccttcaccaa ctacagcagc actaaccagc tgacttttga acgtgcgcag
    29521 gaagtgttgt tagatcgctc ctggcaagca ggcaaaacct ataactttgg tctttacccg
    29581 gcgggcgatg agtggcaact ggcgctcagc gacggcgaaa ccggcaaaaa ttacctctcc
    29641 gacgcgttta aatttggcgg cgagcaaaaa ctgcaactga aagaaacgac cgcccagccc
    29701 gaaggcgaac gcgcgaatct gcgcgtgatt acccagaatc gtcaggcgct gagtgacatt
    29761 accgccattc tgccggatgg caacaaagtg atgatgagct ccctgcgcca gttttctggc
    29821 acgcagccgc tctacacact cgacggtgac ggcacgttga cgaataatca gagtggcgtg
    29881 aaatatcgtc cgaataacca aattggcttt taccagtcca ttaccgccga cggcaactgg
    29941 ggggatgaaa agctaagccc cggttacacc gtgaccaccg gctggaaaaa ctttacccgc
    30001 gtctttaccg acgaaggcat tcagaaaccg ttcctcgcca ttttcgtctg gaccgtggtg
    30061 ttctcgctga tcactgtctt tttaacggtg gcggtcggca tggttctggc atgtctggtg
    30121 cagtgggaag cgttgcgcgg caaagcggtc tatcgcgtcc tgctgattct gccctacgcg
    30181 gtgccatcgt tcatttcaat cttgattttc aaaggattgt ttaaccagag cttcggtgaa
    30241 atcaacatga tgttgagcgc gctgtttggc gtgaagcccg cctggttcag cgatccgacc
    30301 accgctcgca cgatgctgat tatcgtcaat acctggctgg gttatccgta catgatgatc
    30361 ctctgcatgg gcttgctgaa agcgattccg gacgatttgt atgaagcctc agcaatggat
    30421 ggcgcaggtc cgttccagaa cttctttaag attacgctgc cgctgctgat taaaccgctg
    30481 acgccgctga tgatcgccag cttcgccttt aactttaaca acttcgtgct gattcagctg
    30541 ttaaccaacg gcggcccgga tcgtcttggc acgaccacgc cagccggtta taccgacctg
    30601 cttgttaact acacctaccg tatcgctttt gaaggcggcg ggggtcagga cttcggtctg
    30661 gcggcagcaa ttgccacgct gatcttcctg ctggtgggtg cgctggcgat agtgaacctg
    30721 aaagccacgc gaatgaagtt tgattaaggg agataacaaa aatggcaatg gtccaaccga
    30781 aatcgcaaaa agcacgtttg tttattactc acctgctact gctacttttt atcgcggcga
    30841 ttatgttccc gctgctgatg gtcgtcgcta tctcgctgcg tcagggaaac ttcgcgaccg
    30901 gtagcctgat cccggagcaa atctcctggg atcactggaa actggcgtta ggttttagcg
    30961 ttgaacaggc tgatggtcgc attacgccac cgccattccc ggtactgctg tggctgtgga
    31021 actcggtaaa ggtcgctggg atttccgcga ttggcattgt ggcgctctcc accacctgcg
    31081 cctacgcttt cgcccgtatg cgctttccag gcaaagcgac gctgctgaaa ggaatgctga
    31141 ttttccagat gttcccggca gtactttcac tggtcgcgtt gtatgcgttg tttgatcgtc
    31201 tgggtgagta cattccattc attggcctga atactcacgg cggcgtaatt ttcgcgtatc
    31261 tgggtgggat tgcgctgcat gtctggacca tcaaaggcta tttcgaaacc atcgacagtt
    31321 cgctggaaga agcggctgcg ctggatggtg cgacaccgtg gcaggccttc cgccttgtcc
    31381 tgttgccgct gtcagtaccg attctggcgg tggtattcat cctgtcgttt atcgctgcca
    31441 ttactgaagt tccggtcgcg tcgctgttac tgcgtgacgt aaacagctac accctggccg
    31501 tggggatgca gcaatacctc aacccgcaaa actacctgtg gggtgacttt gccgccgctg
    31561 ccgtgatgtc tgcattaccg atctccatcg tcttcttgct ggctcaacgc tggctggtca
    31621 acggcctgac ggcaggtggt gtgaaaggtt aaagatgttg ttctgccaat gttatgccgc
    31681 tgcaccctca acttacgtta tcccaacttg tgactattat tcggcgctcc acggagcgcc
    31741 tttttttctt tcgtctgcaa tctgaatcgt tcgccggtta atattcccat catagaactt
    31801 attattttta cgttatttgt tttcccactt acgataattc tctttcgtgc tctgagtcac
    31861 ggcaatagta ttgtttcatc aatttttgat aattatcaca attaagatca cagaaaagac
    31921 attacgtaaa cgcattgtaa aaaatgataa ttgccttaac tgcctgacaa ttccaacatc
    31981 aatgcactga taaaagatca gaatggtcta aggcaggtct gaatgaatac ccagtataat
    32041 tccagttata tattttcgat taccttagtc gctacattag gtggtttatt atttggctac
    32101 gacaccgccg ttatttccgg tactgttgag tcactcaata ccgtctttgt tgctccacaa
    32161 aacttaagtg aatccgctgc caactccctg ttaggatttt gcgtagccag cgctctgatt
    32221 ggttgcatca tcggcggtgc cctcggtggt tattgcagta accgcttcgg ccgtcgtgat
    32281 tcacttaaga tttctgctgt cctgtttttt atttctggtg taggttctgc ctggccagaa
    32341 cttggtttta cctctataaa cccggacaac acagtaccta tttatctggc aggttatgtc
    32401 ccggaatttg ttatttatcg tattattggc ggtattggcg ttggtttagc ctcaatgctt
    32461 tcaccaatgt atattgcgga actggctcca gctcatattc gcggaaaact ggtttcattt
    32521 aaccagtttg cgattatttt cgggcaactt ttagtttact gcgtaaacta ttttattgcc
    32581 cgttccggtg atgccagctg gctgaatact gacggcaggc gttatatgtt tgcctcggaa
    32641 tgtatccctg cactgctgtt cttaatgctg ctgtataccg tgccagaaag tcctcgctgg
    32701 ctgatgtcac gcggcaagca agaacagacg gaaagtatcc tgcgcaaaat tatgggcaac
    32761 acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg ccgcaaaacc
    32821 ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtaa tcggcgttat gctctccatc
    32881 ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt gttcaaaacg
    32941 ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt tatcaacctc
    33001 acctttaccg tactggcaat tatgactgtg gataaatttg gtcgtaagcc actgcaaatt
    33061 atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt ttacactcag
    33121 gcatcgggta ttgtggcgct actgtcgatg ctgttctatg ttgccgcctt tgccatgtcc
    33181 tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat tcgtggtaaa
    33241 gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc ctggaccttc
    33301 ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt ctcctactgg
    33361 atttacggtt gtatgggcgt tctggcagca ctgtttatgt ggaaatttgt cccggaaacc
    33421 aaaggtaaaa cccttgagga gctggaagcg ctctgggaac cggaaacgaa gaaaacacaa
    33481 caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccatttcggc gtgctgtctt
    33541 tttactcccg cttcagccgt ttcgaattac acagccacag ggtgatcacc agtaacagga
    33601 tcgcagccga gtagatcaac acatccagtg gcgatttatg atcgacgatg atcaagcgca
    33661 caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa ccggactgaa
    33721 agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc agtccttcta
    33781 ccagctcata tttgctggtt tgttctggcg cgaacagcac atcagccaga tgcaccgttt
    33841 ctttgccgag gaagacaacc aaaatcaggc cgaggcacag caggccaaga ttgagtacgg
    33901 tctgcaaaat ggtggagata aactccacgc gcggacgaga gagtgacgtc atagcagtac
    33961 ctccttcaca gtggcgagct tcctgtataa cgcaaaaatg tgacggagat ctatattttt
    34021 tgtgtatgtt ttgttcaacc tggatttgga gcgcgcctcc accaaagccg gatataccta
    34081 cccgtaggcc ggataagccg cgcctggcgg cgcatccggc aagacaaacc gcaccaaagc
    34141 cggtaattca aaaatcaccc attcaggtga ttcccagcca ccagcaatgc gcgaaaaatc
    34201 gacaatgttc ctctggaaca aatgaaacgt aacctgataa aggtggtaaa aatgaagcct
    34261 tattttgctg ctttgatgtt atcagtctct gttcttcctg cttatgctgg tccgctcggt
    34321 actgctgata aagccgacct accgcaaagc aatgtctcca gcccaatgat ggcccagtcg
    34381 ctcaggcaac cagacctgca accgatatcg acggacagga agacagaatg ttttcgcctt
    34441 tacacaccag atcgtaaacc gggggtgaac tgcgttcctg atggctcaac aggtcattaa
    34501 gaatgagggt gccagtgagt gcgaaaactg gcacctttcc catgacttag cggaaattta
    34561 cgctacggtc gctggtcatg tcatacaact ggaacttacg cccaagttgc tgaccaccgt
    34621 cacgcgtcag cggcgtccag ccaatcgccg cacggctgcg tgtcgggcca gacgagaaga
    34681 gatccaacgg taccgagaca tacacgcctt tggtgaagtc cccttcgccg tactcctctt
    34741 tcgaaacgtt ggtgatcgtg gcatagccac ccaccaccac gccgctgtca aagcgtttag
    34801 cgatctccag cgtgccgcct ttatcccctg ccagatactg cccgacgctg gctttcacca
    34861 acacgtcctg ggcgaaagat ggcgtccagt aggcggtcag atgtccggtt ttcacgctgt
    34921 agtcggtgaa tttcatcata tcttttgcac tacgccagtc gcgctgttta acgtagttgg
    34981 catccagacc aaacgcccag ttactgtcca gcggacgata caacacttcg gcccccgcac
    35041 cgccaaacat ggtttcgaga taacctccat agacctgacc gtagaagccg ttgcccagat
    35101 gctggaagta gttggcttgc aggttattca catagacatc gttctgcaca tactcgcgca
    35161 catgggtacg aacgcgcggt aagtgcgagt cctgcggtgg attggtgtag ttaaacttgt
    35221 cgtagttgtt ggcaagattt gcaaacaggc tgccggtggt cagtagatgg tccgtcagcc
    35281 acaaatctgc cgttcccatc acgcccagct gatacatgta aaagttttcc gggccaccga
    35341 ccgactggtt cagcaccgga tcgatatgga aatcaaagcg tgatttgtcg atataccagc
    35401 cctgctcggt ggacttcgga accactggct cgacgcgttt gtgcgccagc gtcgtttcgt
    35461 ggcccaacgg ctctccggcg agattatttt tcaggctggc gacatcggtt tccgtcgtca
    35521 cttgcggcat gttaaggcga ttttccgtaa tgcggatcgt tttgatccca tccggcagat
    35581 cgttcatcac gatccgatta gcacggatga tcccttcgcg cgaatcacga tatttcacct
    35641 gctcgccggt aacatacagc gtatcgcctt tcgcctggat ctgcggatcg gcaagtccgg
    35701 cgttgtattt cagcaacgtt aactgattcg ccaccaccga atgctgcaaa atggcatcct
    35761 gcggctgcgg ttgatattgc gggcgggcgt tatcgatgta ggacgggcgc agatcgttaa
    35821 agttggtgcg cagcgtgacg ccaaacataa aggtgttgcc acgttcatag ctaaggttaa
    35881 cgtcggccca atcggtaacg cgataaatcg ccccgacgtt aaacttactt ttttgctcca
    35941 gcttcccggc aaaatcctgc tgataattat taccttcata ctccagtttc agacgcagcg
    36001 gttgccaggg cgtctggtat tccacgccgc caaacagtga ggcaggaccg tggaacatct
    36061 ggctaccgtc gatagatccc gcctgtttgt agctgttatc gcgatagcaa tatttatcac
    36121 tggctgaaca gagcggattt tttacattac cgctggtgcc cagatatccc cagcccaggc
    36181 cgagcgtaaa atcgaacggc ccccaggctt tgctggcaac aagatattcc gcatcaaaca
    36241 gccccgtacc gccgatatcc cgcgcaccaa ccgccacttg cggcagccag taactctctt
    36301 cccacaaacg cagtttgaga tcgaaggctt tatctttata cgtttgatcg ccagagaacg
    36361 cttcgacgct gctgtactgc cgggtgcgca cgtcggtgta gcgcagcgtt gtttccagcc
    36421 acgggaagag ttgcactgaa gctgagtaat aacggtactg atcgttatcg cgatagttca
    36481 gactcaactc cccttcccgt gccatacgcg cggtgggagt ttgtaataat cctacgccac
    36541 cgaaatccga ctgcgacgga ccaatgggcg caggatatgt ttcgccgtag caagctgtgc
    36601 taatgcccag cgccagtaag ctaagcagat gtcttttttt cattattggg gtatccgctg
    36661 cgtcagagtc tgaagaatgt cggcgttaag ggcatcaggc gtctcactcc agacggaatc
    36721 tgcgaggcca acataaataa tgctgccggg catcggctct acgtgacgct tgttccagta
    36781 agccaccggc gctttttgcg tgcgtccgtc cgggtaaacg acccacgcgt agctgcgatc
    36841 cgcaccgctg agcaggctct ggtcagagag atagctcgcc acgtcgcgac ctggagtgaa
    36901 tggctgattg ccaggatggc tgataagccc gaacaacgtg accgtggacg gcggtggtcc
    36961 gacccacagc gtgtagttgc cctgcaacgg cgggttaccg cgttcggcaa cgcggacgat
    37021 atcgggatca agattgattt tttgcctgcc tgtcactttc aatgcctgga tttgctggcg
    37081 taaggcgtta atggcggcgg catcgtcggt gctggaatct gcgccctgtt ctgccagccg
    37141 tgtcagcaac gcctgctgct gacgcaatgc tgccgccgtt gccagctctt cgctaatcac
    37201 cgcaccaggc caccagctgt ttgccagtcg aggttgccct accagatcga ttaaatgctc
    37261 tgcgcccgtt agcgttttgg cctcactgct gccattgctg aacaccttga cggttcctgc
    37321 cgcgaagacc gatgacgctc ccacactcaa aagcaacgcg acaatagttt gtttaatcat
    37381 tgttttgccg ccttgatgag agtggttttc accgggaaat aatccgcgcc gagatactgt
    37441 tccgactggc gaatttgccc ttcgctgtcg atccagtagc ggttatgcca gcgcgcctgg
    37501 tcggtggaga cttcttcgtc cagcacgcga accagggttt catcgctgcc gaccttgacg
    37561 gtatcggtgc catcccattt gaagactgag cgtgcggtgg cgtagcgtac ctgctggtac
    37621 tcggtccagc ccatcgtgcg cgtccatgtt gcaccatcaa caatttgtgc gggtttaatc
    37681 agcgggtcgg cggcaaggtt attcacttca atcaggttgt cgccgccaag caaagtcttc
    37741 accagacgac catgttgtgt gacaagagtg gcttgatcct gagtgaccca tttttgttgt
    37801 ccgtcttcag cgaaggccag caccacaaac aattgcggcc cgccattaag ctgcatgtac
    37861 tggctggcgt agggcatatt ttgaatatca tcgtccgtca gctgtacgcc tggcgtgccg
    37921 aacagactgt cccacagtga attgcccagc tctttagtcg tggctgaaca ggcctgtaat
    37981 agcaggcaga taagaatgag tgcaggtcgc ttcacgactc ttctccagag gggcagaata
    38041 accacaccgg agtgtggtta tggtaaatcc cttcatactt caagccacag atgcaatgac
    38101 tcaaattatt ttggggatac atactttatt actgggtact ggtggtcgtg gttgtggtgg
    38161 tcccggtatt agaaccatca ccgccaccgg ttgccgcgag cgcgacaccc accgccgagc
    38221 ttacggtgct gacgctggtc gcggaggaac tccccgccga aaccgacgtt gctgccgact
    38281 ctgccgcttc gcccacctgt acaggtgcag cccacgcaga agtcgccgca agcgcagata
    38341 tggcaaaaat gccatacaga acttttttca taacaatttc ccttcattga atgaatggag
    38401 atttacccaa aaacatttcg ggcgggatcg agtatacaca actaaagcat gcgaattacg
    38461 gcgcggggaa ttagcgatgg agttttagga taattggaat gaatatcaaa tataagtttc
    38521 aacttatatt taaataacaa taaaaatcat aagcttaaaa taaaaaacaa aagtaattaa
    38581 tctgcgtatt ctccgaaata aacaacgatt aaaaatctat tttaggatta acctcacggt
    38641 atgatttccg ttaaatcaca gacaagggga tttatctgat aaaacaatgc cgacttattg
    38701 tcggcattgt ttttaaggtt atttaactcc tcatactatg cattaatgtc gatattggca
    38761 tcaggctccg gttgccggat gcggcgtaaa cgccttatcc ggcctacata tcggcatcag
    38821 gcacgcatga tgattaaccg cgccacgctt tatagcggtt aatcagacca ttggtcgagc
    38881 tatcgtggct gctgatttct ttatcatctt tcagctctgg cagaatacgg tttgccagtt
    38941 gtttacccag ctccacgccc cactggtcga aggtgaagat gttcaggatc acgccctgag
    39001 taaagatttt gtgctcatac agcgcaatca acgcacccag gctgaacgga gtgatttcgc
    39061 gcagcaggat ggagttggtc gggcggttac cttcgaacac tttgaacggc accacgtagt
    39121 caagcgttgc cggatcttta ccctgatcgc gatattcctg ctcaaccact tcgcgggatt
    39181 taccaaacgc cagcgcttcg gtctgggcga agaagttaga cagcagtttc tggtggtgat
    39241 cagagagcgg gttatgggtg atagccgggg cgatgaaatc gcacggtacc attttggttc
    39301 cctggtggat cagctggtag aacgcgtgct gaccgttagt gcctggttca ccccagataa
    39361 tcgggccagt ctggtaatcc acaacgttac cgttacggtc aacatactta ccgttggact
    39421 ccatattgcc ctgctggaag tacgccgcga aacggtgcat atactggtca tacggcagaa
    39481 tcgcttcagt ttccgcacca aagaaattgt tgtaccagat gccaatcagc gccagcagga
    39541 caggcaggtt tttctcggca ggcgtggtgg agaaatgctt gtccattgcg tgtgcgccag
    39601 aaagcagttc aacgaagtta tcaaagccga tggagagaac aatcgacagg ccaatcgctg
    39661 accacaaaga gtaacggccg ccgacccagt cccagaactc gaacatgttg gcagtatcaa
    39721 taccaaactc gccaacggct ttggcattgg ttgagagcgc cgcaaaatgt ttcgcaacgt
    39781 gtttttcatc acctgccgct ttcaggaacc agtcacgcgc gctatgggcg ttggtcatgg
    39841 tttcctgagt ggtgaaggtt ttagatgcta ccaggaacag cgtggtttcc gggtttactt
    39901 ttttcagcac ttccgagatg tgagtcccat cgacgttaga aacaaagtgc atgttcaggt
    39961 ggtttttgta cggacgcagt gcttcggtca ccatgtacgg gccgaggtca gaaccgccga
    40021 tcccgatgtt cactacgtca gtgattgctt tgccggtata acccttccac tcaccggaga
    40081 taatcgcttc cgagaaggtt ttcatcttct ccagcaccgc gttgacttct ggcataacat
    40141 ctttgccatc aaccagaatc ggggtattgc tacgattacg cagcgctacg tgcagcacgg
    40201 cgcggttttc agtgcggttg atcttctcgc cagagaacat cgacttaatc gcgcccgcca
    40261 gatcgcactc tttcgccaga tcctgtaatt tcgccagcgt ctcttcagtg atgcggtttt
    40321 tggagtaatc caccagcatc tgatcgtcga aggttgcgga gaacttagaa aaacgatcgc
    40381 cgtctttagc aaaaagatcg gcgatcgtaa cgtctttcat ttcatcgaag tgtttctgta
    40441 gtgcctgcca ggcagcggtc tgcgttggat tgatgttttt cattagcaat actcttctga
    40501 ttttgagaat tgtgactttg gaagattgta gcgccagtca cagaaaaatg tgatggtttt
    40561 agtgccgtta gtgtaatgtt gagtgtaaac ccttagcgca gtgaagcatt tattagctga
    40621 actactgacc gccaggagtg gatgaaaaat ccgcatgacc ccgtcgttga caaccgcccc
    40681 gctcaccctt tatttataaa tgtactacct gcgctagcgc aggccagaag aggcgcgttg
    40741 cccaagtaac ggtgttggag gagccagtcc tgtgataaca cctgaggggg tgcatcgccg
    40801 aggtgattga acggctggcc acgttcatca tcggctacag gggctgaatc ccctgggttg
    40861 tcaccagaag cgttcgcagt cgggcgtttc gcaagtggtg gagcacttct gggtgaaaat
    40921 agtagcgaag tatcgctctg cgcccacccg tcttccgctc ttcccttgtg ccaaggctga
    40981 aaatggatcc cctgacacga ggtagttatg tctgaaattg ttgtctccaa atttggcggt
    41041 accagcgtag ctgattttga cgccatgaac cgcagcgctg atattgtgct ttctgatgcc
    41101 aacgtgcgtt tagtagtcct ctcggcttct gctggtatca ctaatctgct ggtcgcttta
    41161 gcggaagggc tggaacctgg cgagcgattc gaaaaactcg acgctattcg caacatccag
    41221 tttgccattc tggaacgtct gcgttacccg aacgttatcc gtgaagagat tgaacgtctg
    41281 ctggagaaca ttactgttct ggcagaagcg gcggcgctgg caacgtctcc ggcgctgaca
    41341 gatgagctgg tcagccacgg cgagctgatg tcgaccctgc tgtttgtcga aatcctgcgc
    41401 gaacgcgatg ttcaggcaca gtggtttgat gtacgtaaag tgatgcgtac caacgaccga
    41461 tttggtcgtg cagagccaga tgtagccgcg ctggcggaac tggccgcgct gcagctgctc
    41521 ccacgcctca atgaaggctt agtgatcacc cagggattta tcggtagcga aaataaaggt
    41581 cgtacaacga cgcttggccg tggaggcagc gattatacgg cagccttgct ggcggaggct
    41641 ttacacgcat ctcgtgttga tatctggacc gacgtcccgg gcatctacac caccgatcca
    41701 cgcgtagttt ccgcagcaaa acgcattgat gaaatcgcgt ttgccgaagc ggcagagatg
    41761 gcaacttttg gtgcaaaagt actgcatccg gcaacgttgc tacccgcagt acgcagcgat
    41821 atcccggtct ttgtcggctc cagcaaagac ccacgcgcag gtggtacgct ggtgtgcaat
    41881 aaaactgaaa atccgccgct gttccgcgcg ctggcgcttc gtcgcaatca gactctgctc
    41941 actttgcaca gcctgaatat gctgcattct cgcggcttcc tcgcggaagt tttcggcatc
    42001 ctcgcgcggc ataatatttc ggtagactta atcaccacgt cagaagtgag cgtggcatta
    42061 acccttgata ccaccggttc aacctccact ggcgatacgt tgctgacgca atctctgctg
    42121 atggagcttt ccgcactgtg ccgggtggag gtggaagaag gtctggcgct ggtcgcgttg
    42181 attggcaatg acctgtcaaa agcctgcggc gttggcaaag aggtattcgg cgtactggaa
    42241 ccgttc
//
LOCUS       DAJTRM010000025        40546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRM010000025 DAJTRM010000000
VERSION     DAJTRM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.025"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..956)
                     /locus_tag="OO322_001447"
     CDS             complement(<1..956)
                     /locus_tag="OO322_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5906382.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO322_001448"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO322_001448"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5906383.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO322_001449"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO322_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO322_001450"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO322_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5906384.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO322_001451"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO322_001451"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5906385.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO322_001452"
     CDS             complement(5018..6346)
                     /locus_tag="OO322_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5906386.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="OO322_001453"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="OO322_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO322_001454"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO322_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5906387.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO322_001455"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO322_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5906388.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO322_001456"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO322_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5906389.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO322_001457"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO322_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5906390.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO322_001458"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO322_001458"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5906391.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="OO322_001459"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="OO322_001459"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5906392.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="OO322_001460"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="OO322_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5906393.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO322_001461"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO322_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5906394.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="OO322_001462"
     CDS             18267..19166
                     /locus_tag="OO322_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5906395.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="OO322_001463"
     CDS             19172..21604
                     /locus_tag="OO322_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5906396.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="OO322_001464"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="OO322_001464"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5906397.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="OO322_001465"
     CDS             22717..23982
                     /locus_tag="OO322_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5906398.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="OO322_001466"
     CDS             complement(24223..25815)
                     /locus_tag="OO322_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5906399.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="OO322_001467"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="OO322_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5906400.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="OO322_001468"
     CDS             complement(27012..28130)
                     /locus_tag="OO322_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5906401.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO322_001469"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO322_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5906402.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="OO322_001470"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="OO322_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5906403.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="OO322_001471"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="OO322_001471"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5906404.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO322_001472"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO322_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5906405.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="OO322_001473"
     CDS             complement(31379..31444)
                     /locus_tag="OO322_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906406.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="OO322_001474"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="OO322_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5906407.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="OO322_001475"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="OO322_001475"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5906408.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="OO322_001476"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="OO322_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5906409.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="OO322_001477"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="OO322_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5906410.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="OO322_001478"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="OO322_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5906411.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="OO322_001479"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="OO322_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO322_001480"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO322_001480"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5906412.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="OO322_001481"
     CDS             39719..39979
                     /locus_tag="OO322_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5906413.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40546
                     /locus_tag="OO322_001482"
     CDS             40244..>40546
                     /locus_tag="OO322_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5906414.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10096 a        10718 c         9867 g         9865 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgct
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgag ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcgtg
//
LOCUS       DAJTRM010000026        39023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRM010000026 DAJTRM010000000
VERSION     DAJTRM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.026"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..159
                     /locus_tag="OO322_001483"
     CDS             <1..159
                     /locus_tag="OO322_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5906415.1"
                     /translation="EIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVS
                     GGGVQELN"
     gene            388..978
                     /gene="uidR"
                     /locus_tag="OO322_001484"
     CDS             388..978
                     /gene="uidR"
                     /locus_tag="OO322_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5906416.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1366..3069
                     /gene="uidA"
                     /locus_tag="OO322_001485"
     CDS             1366..3069
                     /gene="uidA"
                     /locus_tag="OO322_001485"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5906417.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3075..4100
                     /gene="uidB"
                     /locus_tag="OO322_001486"
                     /pseudo
     CDS             3075..4100
                     /gene="uidB"
                     /locus_tag="OO322_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4139..5404
                     /gene="uidC"
                     /locus_tag="OO322_001487"
     CDS             4139..5404
                     /gene="uidC"
                     /locus_tag="OO322_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5906418.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5630..7138)
                     /locus_tag="OO322_001488"
     CDS             complement(5630..7138)
                     /locus_tag="OO322_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5906419.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7239..8414)
                     /gene="manA"
                     /locus_tag="OO322_001489"
     CDS             complement(7239..8414)
                     /gene="manA"
                     /locus_tag="OO322_001489"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5906420.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8613..10259
                     /gene="fumB"
                     /locus_tag="OO322_001490"
     CDS             8613..10259
                     /gene="fumB"
                     /locus_tag="OO322_001490"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5906421.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10402..11805
                     /gene="fumC"
                     /locus_tag="OO322_001491"
     CDS             10402..11805
                     /gene="fumC"
                     /locus_tag="OO322_001491"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5906422.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11802..12731)
                     /gene="tus"
                     /locus_tag="OO322_001492"
     CDS             complement(11802..12731)
                     /gene="tus"
                     /locus_tag="OO322_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5906423.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12807..14108)
                     /gene="rstB"
                     /locus_tag="OO322_001493"
     CDS             complement(12807..14108)
                     /gene="rstB"
                     /locus_tag="OO322_001493"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5906424.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14287..15606)
                     /locus_tag="OO322_001494"
     CDS             complement(14287..15606)
                     /locus_tag="OO322_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5906425.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15831..16574)
                     /gene="rstA"
                     /locus_tag="OO322_001495"
     CDS             complement(15831..16574)
                     /gene="rstA"
                     /locus_tag="OO322_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5906426.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16703..17038
                     /locus_tag="OO322_001496"
     CDS             16703..17038
                     /locus_tag="OO322_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5906427.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17035..17757)
                     /gene="folM"
                     /locus_tag="OO322_001497"
     CDS             complement(17035..17757)
                     /gene="folM"
                     /locus_tag="OO322_001497"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5906428.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17794..19176)
                     /locus_tag="OO322_001498"
     CDS             complement(17794..19176)
                     /locus_tag="OO322_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5906429.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19362..20306)
                     /locus_tag="OO322_001499"
     CDS             complement(19362..20306)
                     /locus_tag="OO322_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5906430.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20830..22362
                     /gene="pntA"
                     /locus_tag="OO322_001500"
     CDS             20830..22362
                     /gene="pntA"
                     /locus_tag="OO322_001500"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5906431.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22373..23761
                     /gene="pntB"
                     /locus_tag="OO322_001501"
     CDS             22373..23761
                     /gene="pntB"
                     /locus_tag="OO322_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5906432.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23786..24820)
                     /gene="tqsA"
                     /locus_tag="OO322_001502"
     CDS             complement(23786..24820)
                     /gene="tqsA"
                     /locus_tag="OO322_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5906433.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25231..25596
                     /gene="mdtJ"
                     /locus_tag="OO322_001503"
     CDS             25231..25596
                     /gene="mdtJ"
                     /locus_tag="OO322_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5906434.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25583..25912
                     /gene="mdtI"
                     /locus_tag="OO322_001504"
     CDS             25583..25912
                     /gene="mdtI"
                     /locus_tag="OO322_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5906435.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25951..26772)
                     /locus_tag="OO322_001505"
     CDS             complement(25951..26772)
                     /locus_tag="OO322_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5906436.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26872..26955)
                     /locus_tag="OO322_001506"
     CDS             complement(26872..26955)
                     /locus_tag="OO322_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906437.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27048..27383)
                     /locus_tag="OO322_001507"
     CDS             complement(27048..27383)
                     /locus_tag="OO322_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5906438.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27780..29033)
                     /locus_tag="OO322_001508"
                     /pseudo
     CDS             complement(27780..29033)
                     /locus_tag="OO322_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29140..30051
                     /locus_tag="OO322_001509"
     CDS             29140..30051
                     /locus_tag="OO322_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5906439.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30186..31406
                     /gene="mlc"
                     /locus_tag="OO322_001510"
                     /gene_synonym="dgsA"
     CDS             30186..31406
                     /gene="mlc"
                     /locus_tag="OO322_001510"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5906440.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31531..32226
                     /gene="bioD"
                     /locus_tag="OO322_001511"
     CDS             31531..32226
                     /gene="bioD"
                     /locus_tag="OO322_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5906441.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32179..33471)
                     /gene="clcB"
                     /locus_tag="OO322_001512"
     CDS             complement(32179..33471)
                     /gene="clcB"
                     /locus_tag="OO322_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5906442.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33630..34244)
                     /gene="dmsD"
                     /locus_tag="OO322_001513"
     CDS             complement(33630..34244)
                     /gene="dmsD"
                     /locus_tag="OO322_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5906443.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34287..35141)
                     /locus_tag="OO322_001514"
     CDS             complement(34287..35141)
                     /locus_tag="OO322_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5906444.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35143..35760)
                     /gene="dmsB"
                     /locus_tag="OO322_001515"
     CDS             complement(35143..35760)
                     /gene="dmsB"
                     /locus_tag="OO322_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5906445.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35771..37809)
                     /locus_tag="OO322_001516"
                     /pseudo
     CDS             complement(35771..37809)
                     /locus_tag="OO322_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37870..38979)
                     /locus_tag="OO322_001517"
     CDS             complement(37870..38979)
                     /locus_tag="OO322_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5906446.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9918 a         9447 c         9987 g         9671 t
ORIGIN      
        1 gaaattgaac aaaaaatgtt acagcacacg ccgatcagac gtctgggcca accgcaagat
       61 attgctaacg cggcgctgtt cctttgctcg cctgcagcca gctgggtaag cggacaaatt
      121 ctcaccgtct ccggtggtgg ggtacaggag ctcaattaat acactaacga accggtaaac
      181 ggtcgtatgt gttgtttacc gggatagcgt gtgcaattca tcaacgtctc tgctaaacaa
      241 ctggcagcca aatcacggct attggttaac caattgctgg gtgaaaagta tacgaataga
      301 gtgtaccttc gcactattca acagcaatga taggcgctca cctgacaacg cggtaaacta
      361 gttattcacg ctaactataa tggtttaatg atggataaca tgcagactga agcacaaccg
      421 acacggaccc ggatcctcaa tgctgccaga gagatttttt cagaaaatgg atttcacagt
      481 gcctcgatga aagccatctg taaatcttgc gccattagtc ctgggacgct ctatcaccat
      541 ttcatctcca aagaagcctt gattcaggcg attatcttac aggaccagga gagggcgctg
      601 gctcgtttcc gggaaccgat tgaagggatt cattttgttg actatatggt cgagtctatt
      661 gtctctctca cccatgaagc ctttgggcaa cgggcgctgg tggtcgaaat tatggcggaa
      721 gggatgcgta acccacaggt cgccgccatg cttaaaaata agcatatgac gatcactgaa
      781 tttgttgccc agcggatgcg tgatgcccag caaaaaggcg agataagccc agacatcaac
      841 acggcaatga cttcacgttt actgctggat ctgacctacg gtgtactggc cgatatcgaa
      901 gcggaagacc tggcgcgtga agcgtcgttt gctcagggat tacgcgcgat gattggcggt
      961 atattaaccg catcctgatc ctctcatttc gcgggctggt gataactgtg cccgcgtttc
     1021 atatcgtaat ttctctgtgc aaaaattatc ctccccgtgt tcggagaatt cccccccaaa
     1081 atattcaccg tagccatatg tcatgagagt ttatcgttcc caacactctc gaacgaacgt
     1141 tcgattgctt attttatggc ttctgtcaac gctgttttaa agattaatgc gatccatatc
     1201 acgctgtggg tattgcagtt tttggttttt tgatcgcggt gtcagttctt tttatttcca
     1261 tttctcttcc atgggtttct cacagataac tgtgtgcaac acagaattgg ttaactaatc
     1321 agattaaagg ttgaccagta tttttatctt aatgaggagt cccttatgtt acgtcctgta
     1381 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
     1441 gaaaactgtg gaattgatca gcgttggtgc gaaagcgcgt tacaagaaag ccgggcaatt
     1501 gcagtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgtgggc
     1561 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
     1621 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
     1681 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
     1741 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
     1801 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
     1861 aactatgccg ggatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
     1921 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
     1981 gtggtggcca atggtgatat cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
     2041 actggacaag gcaccagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
     2101 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
     2161 ccgctgcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac
     2221 aaaccgttct actttactgg ctttggtcgt catgaagatg cggatttgcg aggcaaagga
     2281 ttcgataacg tgctgatggt gcacgatcac gcattaatgg actggattgg ggccaactcc
     2341 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     2401 atcgtggtga ttgatgaaac tgcagctgtc ggctttaacc tctctttagg cattggtttc
     2461 gaagcgggca acaagccgaa agaactgtac agcgaagagg ctgtcaacgg ggaaactcag
     2521 caggcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     2581 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc
     2641 gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat
     2701 gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg
     2761 aaccgttatt acggttggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg
     2821 gaaaaagaac tgctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac
     2881 ggcgtggata cgttagccgg cctgcactca atgtacaccg acatgtggag tgaagagtat
     2941 cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt
     3001 gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt
     3061 aacaattgac ctacatgggc ctcgggcttt gctacagcct ggtgaatatt ccttatggtt
     3121 cacttgctac cgcgatgacc caacaaccac aatctcgcgc ccgtctgggc gcggctcgtg
     3181 ggattgccgc ttcattgacc tttgtatgcc tggcatttct gataggaccg agcattaaga
     3241 actccagccc ggaagagatg gtgtcggtat accatttctg gacgattgtg ctggtgattg
     3301 ccggaatggt gctttacttc atctgcttca aatcgacgcg tgaaaatgtg gtacgtatcg
     3361 tggcgcagcc gtcattgaag atcagtttgc aaaccctgaa acggaatcgc ccgctgttta
     3421 tgttgtgcat cggtgcgctg tgtgtgctga tttcgacctt cgcggtcagc gcctcgtcgt
     3481 tgttctacgt gcgctatgtg ttaaatgata ccgggctgtt cactgtgctg gtactggtgc
     3541 aaaacctggt tggtactgtg gcatcggcac cgcttgtgcc ggggatggtc gcgaggatcg
     3601 gtaaaaagaa taccttcctg attggcgctt tgctgggaac ctgcggttat ctgctattct
     3661 tctgggtttc cgtctggtca ctgccggtgg cgttggttgc gttggccatc gcttcaattg
     3721 gtcagggcgt tactatgact gtgatgtggg cactggaagc tgataccgta gaatacggtg
     3781 aatacctgac cggcgtgcga attgaagggc tcacctattc actattctca tttacccgta
     3841 aatgcggtca ggcaatcgga ggttcaattc ctgcctttat tttgggatta agcggatata
     3901 tcgccaatca ggtgtaaacg ccggaagtta ttatgggcat ccgcacatca attgccttag
     3961 taccttgcgg atttatgcta ctggcattcg ttattatctg gttttatccg ctcacggata
     4021 aaaaattcaa agaaatcgtg gttgaaattg ataatcgtaa aaaagtgcag cagcaattaa
     4081 tcagcgatat cactaattaa tattcaataa aaataatcag aacatcaaag gtgcaactat
     4141 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
     4201 ttacgcggcg caactggctg acgatgaagc gggactacgc atcagactga aaaacgaatt
     4261 gcgcagggct gataagccca gtgctggcgc gggaagagat atttacgcat gggtacaggg
     4321 aggattgctc gatttcaata gtggttatta ttccaatatt gttggcgttg aaggcggagc
     4381 gtattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
     4441 tgataaaagt tttggctttg ccctgggggc agtaaaaata aaacccagtg aaaatagcct
     4501 gcttaaatta ggtcgcttcg ggacggatta tagttatggt agcttacctt atcgtattcc
     4561 gttaatggtt ggcagttcgc aacgtacatt accgacagtt tctgaaggag cattaggtta
     4621 ttgggcttta acgccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
     4681 gactgattca acaaccggta ttcgtgatga aggggtgtat aacagccaga cgggaaaata
     4741 cgataaacat cgcgcacgtt cttttttagc cgccagttgg tatgatgata ccagtcgcta
     4801 ttctctgggg gcatcggtac agaaagatgt ttccaatcag atacaaagta ttctcgagaa
     4861 aagcataccg ctcgacccga attatacgtt gaaaggggag ttgctcggct tttacgcgca
     4921 gctcgaaggt ttaagtcgta ataccagcca gcccaatgaa acggcgttgg ttagtggaca
     4981 attgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcgccatgc
     5041 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
     5101 taaccgtgaa ggaatgcagt cctggcaatt aggtgccaac taccgtgtaa ctccgcaatt
     5161 tacgctgaca tttgcaccga ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg
     5221 gattgaaggc gcgggtatct taggtggtat gaactatcgg gtcagcgaag ggccgttaca
     5281 agggatgaat ttctttcttg ctgccgataa agggcgggaa aagcgcgatg gcagtacgct
     5341 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
     5401 gtaagaaata acgccggaga gaaaaacctc cggcgtttca gattgttgac aaagtgcgcg
     5461 ttttttatgc cggatgcggc gtaaacgcct tatccagcct acaaaaactc ataaattcaa
     5521 agtgttgcag gaaaaggtag gcctgataag cgtagcgcat caggcaatct ctggtttgtt
     5581 ttcagatgaa aacgccggag tgaaaattct ccggcgtttt ggccgtgaat tactgctgcg
     5641 gaattgccgg tacagccgga acgttaagag ctggcatcgc aaacatgcca acaaaatctt
     5701 ctaacgacat tttctgccca tttaacgtta tctgaccgtt agcatattgc aggctggtgg
     5761 tgatggtatt gtcctgcaag gtggtcagac ggaacatctg ccccattgct gatgcacctt
     5821 caacttgctg tttcgccagt tttttcgctt gatcttcctg ataaccttcc agcttcgcta
     5881 cctgagtcat caactcagtt gccatatcca ccggaatggt cagtttcgca tccagagatt
     5941 taaccgaacg atctacttcc tgcgccagcg tttgcggcgc ttctttagtc gttgccggat
     6001 ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg actgtttttc cagcttagcg
     6061 gcgcgatagt aatcaccgga tcgcctttca acatcagcgg cagggcgcta aagaaggctt
     6121 ccgtcacttt ctcctgataa agttcgggat tgttggcaat ttctggctgt gccagcagcg
     6181 cctgagtttg cgcgttatat tgctggctaa actgatgcca ggcttcacca tcaatctggc
     6241 cgacttttaa agtcagcttg ccgctgccca gatcctgatt ctgtaccttc aggctgttta
     6301 gcgagtaatc cagttggcta ttgatcgttt taccgtcatt gaccagatcc gatttaccgc
     6361 tgatctccat gccttccagc agtgccagtt ctttgccttc cactgaaatg gtcatttttt
     6421 ccagtgacag tttttggttt cctacgcgct caccaaaact tcccagcgtg ctggaaccgt
     6481 cggttttcag attattaaag gtcaactgca ctttctggtt gtattcgtta actgcgtcta
     6541 tccgaccact ttgcgcctct ccggaaaggg agatggcttt gccgtctctg tcagcattta
     6601 actggaactc gccgccgcta aaggcgactt tttcatcctt ttgctcgtaa ttcagtggat
     6661 tgagcgaaat atcggaactg gaatcaccgc tgtaaccaat gcgcgagtta atctcaaaag
     6721 gcgtttcacc ttttgccata tcaaacagtg gtttgcttac ttcgttatta accagcgtgg
     6781 tttgaattga tgccatcgac gggatcaggt tcagtttttt aagctgggca agcgggaagg
     6841 gaccatgatc aaccgattcg ttgaagatga cgctctgacc gcttttaatc cacggatttt
     6901 ctttcccggc aatgggtttc accaacagtt gcaactggct gctgaatacg ccgcgatgat
     6961 agttttgata actcacttcc aggttggatt caggagctgt cagtttgagt tgcgcgttcg
     7021 cctgcgcgac catgtcttcg agatgggttt caatcttctt gcctgtatac catgcgccgc
     7081 ctgtccagac tacgcctagc gcaacaatga cgcctaccgc taccagcgat ttattcataa
     7141 tgattatcca taaaatgaaa tcaggcggac tggccgcctg aaggtgttat aagcctttaa
     7201 taagcttagc aagagatgtt aattttttca gtaagctctt acagcttgtt gtaaacacgc
     7261 gctaaacggc cgtggccttt gacagtcacc ggtgattcgt tggcggcaat aaacgctgat
     7321 tcacccggtt taagctgtaa ctgctgagaa cctttccaca acgttgcatc gccttcgacg
     7381 cagaacaaaa tggcggcact ctgctggctg atggtggttt ctttatcact aaggtcatgc
     7441 agcgagaagg caaaatcatc cacaggaatc gggaagtcca gttctgcacc ttgtttcacc
     7501 ggctgggtca acaactggtt agccggtttg gcttcgaatt tcacattggc aaccagttcc
     7561 ggaatatcaa tgtatttagg cgtcagacct gcacgcagca cgttatcgga gttcgccatc
     7621 acttccagcg ccacgccttg caggtaagcg tgcggtgttt cagcgaacag gaacatcgct
     7681 tcgccaggat tcaatttcac cacattcagt aacagcgggg agaacaggcc gctgtcttcc
     7741 gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt caccctgctg actgtcgagc
     7801 gccgatttta aaatcgccag cgcgcgggat ttttcttcac cctgcatatt caacaggttg
     7861 gcgaacagtt cgcttaaacg ttcggcatgt ggctgttgta aaaagtgagc aatcgccgga
     7921 tgtgcaccgg cgaccggctg gagtagggag acaatctcgg aaaattcacg aaacgcgttc
     7981 atcgcaagga aaggcgtcag cgcaaaaacc agctccggct tgtggttagg atctttatag
     8041 ttacgctcgg cggcatccat cgggatacct gcggcatttt ctttggcaaa accgatttca
     8101 gaattgtgtt tgtttggatg aacctgaatg gagagtggct gtgctgcgca taatactttg
     8161 aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct ctccgagcag agtcgattta
     8221 tcactctcaa tcacatcacg cagtgaaacg atatctccgg cggcattctg cactcgtgaa
     8281 ctgcttttcg gatgtgcgcc catccacagc tcggccatcg gctggctgga cggattttcc
     8341 ataccataaa gttcagtcaa cgccgttttg ctaccccagg catagttttg cactgagtta
     8401 atgagttttt gcatgatcaa tccctgtttt aatgtggaaa ttaatcccgc tattaaagca
     8461 agaatcctac gggaagtaac ctggagccgc aaaaagtcgt actagtctca gtttttgtta
     8521 aaaaactgtg taggatattg ttactcgctt ttaacagggc aacggaacac ccgcccagag
     8581 cataaccaaa ccaggcagta agtgagagaa caatgtcaaa caaacccttt cattatcagt
     8641 ctccttttcc actcaaaaaa aatgatactg agtattacct gctaaccagt gaacacgtta
     8701 gcgtatctga atttgaaggg caggagattt tgaaagtcgc acccgaagcg ttaactctgt
     8761 tggcgcgtca ggcgtttcat gatgcgtcgt ttatgctgcg tccggctcac caacaacagg
     8821 tggccgacat tctgcgtgac ccggaggcca gcgaaaatga taaatatgtg gcgctgcaat
     8881 tcctgcgtaa ctccgacatc gcggcgaaag gcgttctgcc aacctgtcag gataccggca
     8941 ccgcgattat tgttggtaaa aaagggcagc gtgtatggac cggtggtggt gatgaagcgg
     9001 cgctggcgcg cggtgtctat aacacttata tcgaagataa tctgcgctac tcgcaaaacg
     9061 cgccgctgga tatgtataaa gaagtgaata ccggcaccaa tctgccagcg cagatcgatc
     9121 tttatgccgt tgatggcgac gaatacaaat tcctctgtat cgcaaaaggt ggtggttcgg
     9181 caaacaagac gtatctctat caggaaacca aagcgttact gacgccgggg aaactgaaaa
     9241 attacctggt tgagaagatg cgcacgctgg gtaccgcggc ctgtcctccg tatcatattg
     9301 cgttcgttat tggtggaact tctgcagaaa cgaaccttaa aacggtgaaa ctggcttccg
     9361 cgaaatacta tgatgaactg ccaacggaag ggaatgagca cggtcaggcg ttccgcgatg
     9421 tggaactgga aaaagaatta ctgatcgaag cgcaaaatct cggtctgggt gcgcagtttg
     9481 gtggtaaata cttcgctcac gacatccgcg tgattcgcct gccacgtcac ggcgcatcct
     9541 gtccggttgg tatgggtgtt tcctgctctg ctgaccgtaa tatcaaagcg aagatcaacc
     9601 gtcaggggat ctggatcgaa aaactggaac ataatccagg taaatatatc cctgaagagc
     9661 tgcgtaaagc gggagaaggc gaagcggtgc gcgttgacct taaccgtccg atgaaagaga
     9721 tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt atcgcttaac ggcacgatta
     9781 tcgttggtcg tgatattgct cacgccaaac tgaaagagcg gatggataac ggtgaagggc
     9841 tgccgcaata cattaaagat catccgattt actacgcggg tccggccaaa acgccggaag
     9901 gttatgcctc cggttctctt ggcccaacga ccgccggacg gatggattct tatgtcgatc
     9961 aactgcaagc gcagggcgga agtatgatca tgctggcgaa aggcaaccgc agccagcagg
    10021 tgacggatgc ctgtaaaaaa cacggcggct tctaccttgg cagtatcggt ggtccggccg
    10081 ctgtattggc gcagggaagt attaagagcc tggaatgtgt tgaatatccg gaactgggaa
    10141 tggaagccat ctggaaaatt gaagtggaag atttcccggc gtttatcctt gtggatgata
    10201 aaggaaatga cttcttccag cagatacaac tcacacagtg cactcgctgt gtgaaataaa
    10261 cagagccgcc cttcggggcg gtttttttac atggcacgaa agaccaaaca tttgttatca
    10321 aatagtaaat aatgagtgag ctaaaagttg cttaacgaaa gcaaaacaga aagaaaaaat
    10381 taatcaggtg aggagcaggc catgaataca gtacgcagcg aaaaagattc gatgggggcg
    10441 attgatgtcc cggcagataa gctgtggggc gcacaaactc aacgctcgct ggagcatttc
    10501 cgcatttcga cggagaaaat gcccacctca ctgattcatg cgctggcgct aaccaagcgc
    10561 gcagcggcaa aagttaatga agatttagac ttgttgtctg aagagaaagc gagcgccatt
    10621 cggcaggcgg cggatgaagt actggcagga cagcatgacg acgaattccc gctggctatc
    10681 tggcagaccg gctccggcac gcaaagtaac atgaacatga acgaagtgct ggctaaccgg
    10741 gccagtgaat tactcggcgg tgtgcgcggg atggaacgta aagttcaccc taacgacgac
    10801 gtgaacaaaa gccaaagttc caacgatgtc tttccgacgg cgatgcacgt tgcggcgctg
    10861 ctggcactgc gcaagcaact cattccgcag cttaaaaccc tgacacagac gctgagtgaa
    10921 aaatcgcgtg catttgccga tatcgtcaaa atcggtcgaa cccacttgca ggacgccacg
    10981 ccgctaacac tagggcagga gatttccggc tgggtagcga tgctcgagca taatctcaaa
    11041 catatcgaat acagcctgcc tcacgtagcg gaactggctc ttggcggtac agcggtgggt
    11101 actggactaa atacccatcc ggagtatgcg cgtcgcgtag cagatgaact ggcagtcatt
    11161 acctgtgcac cgtttgttac cgcgccgaac aaatttgaag cgcttgcgac ctgtgatgcc
    11221 ctggttcagg cgcacggcgc gttgaaaggg ttggctgcgt cactgatgaa aatcgccaat
    11281 gatgtccgct ggctggcctc tggcccgcgc tgcggaattg gtgaaatctc aatcccggaa
    11341 aatgagccgg gcagctcaat catgccgggg aaagtgaacc caacccagtg tgaagcatta
    11401 accatgctct gctgtcaggt aatggggaac gacgtggcga tcaacatggg tggcgcttcc
    11461 ggtaactttg aactgaacgt cttccgtccg atggtgatcc ataatttcct gcaatcggtg
    11521 cgcttgctgg cagatggcat ggaaagtttt aacaaacact gcgcagtggg tattgaaccg
    11581 aatcgtgagc gaatcaatca attactcaat gaatcgctga tgctggtgac tgcgcttaac
    11641 acccacattg gttatgacaa agccgcggag atcgccaaaa aagcgcataa agaagggctg
    11701 accttaaaag ctgcggccct tgcgctgggg tatcttagcg aagccgagtt tgacagctgg
    11761 gtacggccag aacagatggt cggcagtatg aaagccgggc gttaatctgc aacatacagg
    11821 tgcagccgtg gaatgatcaa acgaagcggc tgcgcctgag gtttataacg gtgctgcaca
    11881 ttgtcggcat cgtaatttaa caactcacca acgtccggca cgcccgcgcc attatcccga
    11941 ttaatcagtg caatcagtgg tgtagggcag gcgtgttgga cttgtttttg atctcctttg
    12001 taccagacgc gggcaatcgg ctgcaccttc actggacgtt tgatttttaa cttcgcgttc
    12061 tgtggcaggg cagcgatatc ctgatactct cgctccagtt ttctttgcca ctcctcgcgc
    12121 gtccacggtg cgacactgcg tggtgatttc aggctttttt ccagctgtgc caggacttca
    12181 tcacgatgca aattcttaat gatatgttta ttagcccaac caaagcgtaa agtggcgggg
    12241 tcgtgcagaa cggtgagcgt gcggtaagca ttaagggtga tcagccccgg caaatgacga
    12301 tgcacccatt caaaacgtgc cgcggtgggg agttctgatt caaccgtgac gatatgctcg
    12361 aacgtggtct tgagtttatt gatgtgctga atatgactga ccaacgctgc ttgcgaaagg
    12421 ttatcgacct ggtaacacaa cacgccaggc agacggacag cggccttgct gctgcgattt
    12481 tcggactgtt gttgaataaa taaatggcgg aaatgacgca acgccagcga ctgcgcgtcg
    12541 ttgccgagat gttgttttac ctcaatacga ttaagcggat tatgctcatc ctcttttttt
    12601 acctccggca aagagaacac gcgggcaacc aatagcttgt gttgctcaag atgagcggca
    12661 aatgtagcca gctcttgttc catctggcga aaggtagtgt tgagtcggtc tacgagatcg
    12721 taacgcgcca taatattaac cactttagtt acaacatact tattttatag cacagtcgtg
    12781 gtgacttgac catcgtgcgc gtagtgtcag gcagaggtaa attgcgggat gttatgccat
    12841 aacggccagc taaacgagaa gcgagcacca cccagttcgc tgatgtcaca attaaccgta
    12901 ccgcccattg tcagtgctat tgagtggaca attgccagcc ccagcccgca gccgccggtt
    12961 gagcgatccc ggctgggatc gaggcgaaca aaaggttcaa agatatgttc gcggttttct
    13021 ggggcaatcc ctgggccatc atcctcaaca attaatgtcg ctctattccc cgacagtagc
    13081 aggctggttt caaccgttga atggcagtag cgcagggcgt tattgagcaa attatccagc
    13141 acgcgctcca ttaagcgcat atccaacgcc gcataatggc cttgcatgag cgtttttatc
    13201 cgtaccgttt tatcgggcgt tactgcctga atatctgcca gatgcgttga cagccacaac
    13261 ggcaggtctg gttcgctaag atgaagctcg ttttgtgggc gatcgagtcg ggcataagtc
    13321 agcagctctt caattaaagc ttcaagttga ctgatatcac gattcaacgc ctgggattcg
    13381 gcggcgctca ggttatcgct catctccagt cgataacgca ggcgcactaa cggtgttcgc
    13441 agttcgtgag cgataccgtc aataagctgt tttttgctgg caattaaggc gttgatattg
    13501 tccgccatct ggttaaatgc gataccaagt cgttcaaagc tcgaaccctc atcaaagtgg
    13561 atacgttcat tgagatgccc atcgccaaat cgttgcgccg ctgcttccag ttttaacata
    13621 tcctgccagt gcggacgcat ccagataaac accggaaagg cgagggaaat agcaataaaa
    13681 gcgatcaggg cgatatccag caatcgcatt tgatggaggt aataaagata aggaacagga
    13741 ccaactgcca gcacgtagtg gctgcgcggg atacgctgca aaaacgtgta ctgatcgtcc
    13801 agggcgacaa tttcgccgcc acgcagtcgg tgcatggaaa tatcatcaag atggtattta
    13861 ctcagtggct cgacacgcag atcgaaagag agatttaaat ccatctcttt cagcgtctta
    13921 ccccagtcgt gtggggggat ctctcgcaat tcgctgcgca tcagatacag cgaactgttc
    13981 atcaaatcat ccagcgactg tttgcccgcg cgttcggcgg taaatttgta caccagccca
    14041 accagcagag acatcacaag gaagcagaca aacaataaca ggtaaaactg gataaacaat
    14101 tttttcattg gcatcgcccg caacagaata ctattcgtat taattcacga ctgtggagca
    14161 atgtaaaccg ccaactggat aagaccttgt ttggaagata atgtccggaa cagacggatg
    14221 tatccccggt gtattcatat tcagtaaatt caattttcca ccggggcttg tttattatgc
    14281 taaggtttaa tttaacgtga cgctttgttg ttctttagtc tctctatcct taagaagacg
    14341 ggctgatatt acaccggaca ttattgaacc attcacatta agcgctgttc ttgccatatc
    14401 aacgatcggc tctatggcaa taaaaataac tactaatgcg acagggaaat taagtgttga
    14461 aaggacaatc agcgccgcga atgtcccacc actacctacg cctgctaccc ctattgagcc
    14521 aagcgcgatg gcaggtaaaa gagttgcgag gaattgcaat gagaagggat cgatacccat
    14581 tgttggagca atcatcgcca ccatcacggc aggataaatc ccagcgcagc cattttgtcc
    14641 catgctggaa ccaaaagacg ctgcaatatt agcaatcgta tttggaaccc ccaatttttc
    14701 caatgcagaa atagccagag gaattgaagc cgcactactg cgagaaacaa acgcaaaggt
    14761 aagtacttgc cagacggtct tgtaatagtt aacggggttt ttacctatta acagcaaaat
    14821 gcccgagtga acaataaaca tcacaaaaac ggccagataa caggcgacga tgaacccgag
    14881 caaattggta aactgtgaaa gctgatacgt tgagaagact ttcgccatta gtgccattac
    14941 cccataaggc gtcagcgcaa tgacaatacg gaccattttc attacccata tttgaatcgc
    15001 gttgatcccc gcgctcaatt tttcgccctc tgctggtgca tctttcttta cttttaacag
    15061 cgcaatacca gcaagaattg taaagatgac gatgccaata accgaaacac ttcttgcccc
    15121 ggtcagatca aggaagaggt tggttgggat caatgacgta atgagtcgcg gtaaagatac
    15181 cgcagctgtt ttcgagacgt catcgctggt taaagaggat tgcatatgac caaaggcgct
    15241 ggcatcaagg ccaattgcac tggcaatagc caggccaact aaacctgcaa tcatgactaa
    15301 acacaacata caggcaacaa tagttaaaga catcttgcca ataccagcgc tattttcgag
    15361 cttattaatc gctgaaagaa tcgaaatgaa tatgagtgga acggcaacaa gttttaataa
    15421 attaacataa ccgttaccag caatattata ccattcgata acggtttcac tgcttgtctc
    15481 aggaatgaaa tgcagggaga taccaatgat agcccccagg aaaatcgcga taaaaacact
    15541 tttggaaagg gattcgctct tttgatccgt tttgaaaatc ccccatgagc agatcaaaaa
    15601 aagcacaata tatgtgaaag ttgtaaccgt tttttaagcc tcttgcctca ttcagcacct
    15661 gaaatgccgg aaagagtttg ttgtaaaaaa tctgcgccat aagacgcagg gacgaggcga
    15721 ttatagagaa attttttctc attttcatat caaatgtagg gggtttggaa tttgaataag
    15781 ctcttattaa ttgaattcgt taattgttct ctttttcaga tgaacaaata ttagtcatct
    15841 gattgtttat tgtcactatc ccatgcatga ggcgcaaaaa gatagccttt gttacgcaca
    15901 gttttaatgc gataaggttc tgcggcgtta tcgagcagtt tttttcttaa ccgcgaaata
    15961 gccacgtcca cgctacgatc cagtccgtca taactgacgc cgcgtaaatt tttcagcaat
    16021 gcatcgcggt ccatgatttg cccggcatgc gtagctagtt cccacaataa ttcgaaatca
    16081 gctgtcgaga gcgagatttc agtgttagcc agggtgacta cgcggttgat ggggtcgata
    16141 gttaacgtgc cgaaatgcag ggctttgtag ggagtaagag acgtttcctg aagacctttg
    16201 gtcagtgtgg cttgctcatt ctgacgcaaa tgcaaacgta aacgcgctag caaaacagca
    16261 gggggcgtcg ttttgagaat atagtcgcag gcacccattt ccagtgccag gatgtggttc
    16321 atatcgctat cgagagaggt tagaagaaca atcggtccag accactttgc gcgtaaatca
    16381 cgacaaatgg tcatgccgtc cttgcctggt agcatgatgt cgagtaacac caaatccgga
    16441 ttttctcgca aaatggtttc ttcggcctgg tcgccgcgcg gttctacggt aacctgcata
    16501 tcatgttttg ccaggtacgc ggcaatcagt gaaccgactt ccgcatcatc ttccacaaat
    16561 acgatagtgt tcataacatt cacggtagat ataaaaacgt cacaatacac cgcgctgttt
    16621 ttacttacta tcaatcgttt ctaaacaagt tttcatcagg tattctactg atgacttgtt
    16681 atggaagtgt taaggtaaaa agatggggct ggtaatcaaa gccgcgctcg gtgcgctggt
    16741 agtgctgttg attggtgttt tagcaaaaac gaaaaattat tatatcgccg ggctgattcc
    16801 acttttcccg acctttgcgc ttatcgcgca ttatattgtt gccagcgaac gcggcattga
    16861 agccttacgc gcaaccatca tttttagtat gtggtcgatt attccctatt ttgtttacct
    16921 ggtgtcgctg tggtatttca ccggaatgat gcgcttgccc gccgcttttg ttggttctgt
    16981 tgcgtgttgg gggataagcg catgggtgtt gattatatgc tggataaagc tgcattaacg
    17041 cagatgacga ccgccatcaa gtgggaaact gcgtccggtg acaaagcaac tggtaagtaa
    17101 gtaatcgacc aggtcgatca cttctttctc gccaggcgcg gttttcatca gtgatttatt
    17161 cagcgcctgt tgtcgatatt cggcatcatc atgttcatta aacaggatca gcgatggcgc
    17221 aatagaattc actttcactt ccggtgccag cttgcgggca aacgagcggg tcatattatc
    17281 cagtgccgct ttgcttgcag catacgcaac atgtttgtcg ctaccgcgct ccaccacata
    17341 atcggtaaag tgaatgatat cgctggcggc gtgtccgtgc ccacgcagta atctttccag
    17401 cgcatggttg agcaggtatg gggtattaac gtggatctgc atcatgcaag ccagtacgtc
    17461 ggccagtggc gcacccggtt tttccgcaat ccacgcactg gcgttatgca aaatagcacg
    17521 cagaccatgg gtggttttta gtacttcatc ggcaaacgcc atcacaccgt cgttggtcga
    17581 aaaatcagcc tgaatacact gcgcacctgc attaatcagt ccatcaatgg ctggatagtg
    17641 tgtccgatag ctgacaatca ccggttgctt ttgattaatg aaatgccatg cgagggcgag
    17701 gccgatgcga cgacctccgc cagtaattaa tattggcaag ggctgggttt tacccatcgt
    17761 tatctccttt gctatccaac gatgggatga acgttatccc accagcatcc aggtcgccgg
    17821 aactgaggca attaacagca tgccaatcag caccatttcc tgacggttca gtacattatc
    17881 atgtgtatgc gttttgcgcg catacaggaa aaccagcagc cccggtgcat acagaacaac
    17941 ggataacagc aagtgcattg gaccagaggc gtacaataac cataagccat aaatgcaggc
    18001 accgacacct accgctttat gcagtggacg ggttgcgatt ttcagcaaga acgcgccgac
    18061 caggaaatag ggcaccagaa tcatttctga ggcgatggtc agcaacgtgt tgtaatcgga
    18121 accggtcagc cagatgagta ccagacaaat ttgcacgcag atgttggtca accacagtga
    18181 ggcggatggc gcagcttgtg cgttctggcg cgcaaaaatg cgtgggaatg ctttatgagt
    18241 ggcagccagg aacggaactt ctgccgccat gatggtccag cttaagtacg cgccgcaaac
    18301 ggaaacgatc aaaccggcag cgatgatgat ttcgccccat ggtcccatca tttccaccat
    18361 cagaccggcc attgacgggt tacgaatttc agccagttca ggacgtgcca ccacacccag
    18421 cgaaagcagc gttaccagca agtaaacgcc cagagcggag agaaccgcca gcagtgtcgc
    18481 tttgccaaca tcacgtttat tacgcgcacg cgcagaaaca accacggcac cttccacacc
    18541 aatgaaaacc cacagggtga tcagcatggt gtttttcacc tgttcccaaa caggtacgcc
    18601 aagtgcaagt ccggtgaagt cgagcttgaa ggtatccagt ttgaacatca tcatcgccag
    18661 cacaacaaac agacccagcg gcaacaattt tgccagtgtc gccaccaggt taatgctggc
    18721 agcggtttgc accccgcgca ggatcaaaaa gtgaacaatc cacaataaag ccgatgcacc
    18781 gactatcgat tgccaggtat taccatcgcc aaacaggcgc aattccggcg tgtccgtaaa
    18841 aaagcttaac gcggaaaaaa cgatcaccag ataggagacg ttggcgatga ccgcgcacag
    18901 ccagtatccc catgcggaac aaaagccgat tagctcgcca aacccttcgc gggcataggt
    18961 aaagataccg ccatcaagtt cgggacgaat gcgcgtgagg atcagcatgg caaaggccag
    19021 caataaaatg ccagcgccag taatacccca gccgatgagc agtgctgccg ggctggcaac
    19081 tgccgccata ttttgcggca gactgaaaac acccgcgccc agcattgagc ttaataccag
    19141 cgcggtgagt gcgctcagtc ccagtttctt ttccatcgat atccagtgat taaaacgtga
    19201 taagaagaat aaatattagg gcaaaacgca taaaaatgga gtttgccact gaagcgcggg
    19261 attttacgaa gagatgtgtc tgcatgcaat ggtcgcgggg aaatttgtga ctaaaaatgc
    19321 gaaaggcggc atcaagccgc cttataggag taactgaata gttatttata cagatctgcg
    19381 ctgacggtca ggttattacc acgtttctgc cactggcggg tgatgtggta atacttagca
    19441 cctttcttcg cggcacgttt cgcaacctga taggagactt cggtcatgtt gccgtagttg
    19501 ccagagaatt tgatgctgtc gaacgggacc atcatcgctg ctgtcgcttt gttcagttct
    19561 tcgactttag tgccatccgg gagcgtgacg gtgtaacgcc cgccttttga tgactgggtt
    19621 tcaaagaagc gaccgacttc agaacttggt gatgcggtag tcgcaacacc cgggatctca
    19681 actttcttcg cggcttcgcc accggcagcc agagctgcac gtcctgcttc ggaatctgcc
    19741 gggatcacat ccgggctctg gacgatacgt ttcttagcat cttttttata gatgaatgca
    19801 gtaatacgct ggttgccgcc ctggttggca tcgatttgac gaacgatgta gaaagagtag
    19861 gcacctttcg cttttgccgc tttggtgatg gcatcattga cttctggctg gctacgatag
    19921 aagccctgga cggtgaccgt gtcaaacggt tcaatcagaa cagcctgatc tttcggcagt
    19981 tcgacaacac cgttaattac gcgattactt gtttcttctg ctttttcagc atcggcttta
    20041 tagaggtcag cgaccacacg ccagttaccg ctgttaccaa aatcagaagt gtcgacaaca
    20101 taaaaagagg cggcaccttc tttatctgcg cgacgagaaa cggctttcac cgcttcgcca
    20161 atagcattaa aacgaccggt aaccactaca cggtcaaaag gtttaaccgc tgccgcttgc
    20221 tccggtgtca gttctgttgc tgcgttaacg gagaatgccg tagcagaaag cagtgccgac
    20281 gccaggaggg tgttcttaag cttcataaaa ataatccttc gccttgcgca aaccaggtac
    20341 tggtattgtt attaacgaga aacgtggctg attattgcat ttaaacggtg taactgtctg
    20401 cgtcattttt catatcacat tccttaaacc aattttaatc ctgctcaaat gaccgtctat
    20461 gcttaaaaaa cagccgtatc agcatcatta ctactgaagc aactgaattg tataagttaa
    20521 tttaatgtta agtagtgatt cgtgccgggg cgatgtctcg ttttacccga ccgtcgaaga
    20581 caattatcag tctttatccg gcgttctaag gtgtttatcc cactatcacg gctgaatcgt
    20641 taatattttg cgagttcacg ccgaaatact gatttttggc gctagatcac aggcataatt
    20701 ttcagtacgt tatagggcgt ttgttactaa tttattttaa cggagtaaca tttagctcgt
    20761 acatgagcag cttgtgtggc tcctgacaca ggcaaaccat catcaataaa accgatggaa
    20821 gggaatatca tgcgaattgg cataccaaga gaacggttaa ccaatgaaac ccgtgttgca
    20881 gcaacgccaa aaacagtgga acagctgctg aaactgggtt ttaccgtcgc ggtagagagc
    20941 ggcgcgggtc aactggcaag ttttgacgat aaagcgtttg tgcaagcggg cgctgaaatt
    21001 gtagaaggga atagcgtctg gcagtcagag atcattctga aggtcaatgc gccgttagat
    21061 gatgaaattg cgttactgaa tcccgggaca acgctggtga gttttatctg gcctgcgcag
    21121 aatccggaat taatgcaaaa acttgcggaa cgtaacgtga ccgtgatggc gatggactct
    21181 gtgccgcgta tctcacgcgc acaatcgctg gacgcactaa gctcgatggc gaatatcgcc
    21241 ggttatcgcg ccattgttga agcggcacat gaatttgggc gcttctttac cgggcaaatc
    21301 actgcggccg ggaaagtgcc accggcaaaa gtgatggtga ttggtgccgg tgttgcaggt
    21361 ttggccgcca ttggcgcagc aaacagtctc ggcgcgattg tgcgtgcatt cgacacccgc
    21421 ccggaagtga aagaacaagt tcaaagtatg ggcgcggaat tcctcgagct ggattttaaa
    21481 gaggaagcgg gcagcggcga tggctatgcc aaagtgatgt cggacgcgtt catcaaagcg
    21541 gaaatggaac tctttgccgc ccaggcaaaa gaggtcgata tcattgtcac caccgcgctt
    21601 attccaggca aaccagcgcc gaagctaatt acccgtgaaa tggttgactc catgaaggcg
    21661 ggcagtgtga ttgtcgacct ggcagcccaa aacggtggca actgtgaata catcgtgccg
    21721 ggtgaaatct tcagtacgga aaatggtgtc aaagtgattg gttataccga tcttccgggc
    21781 cgtctgccga cgcaatcctc acagctttac ggtactaacc tcgttaatct gctgaaactg
    21841 ttgtgcaaag agaaagacgg caatatcact gttgattttg atgatgtggt gattcgcggc
    21901 gtgaccgtga tccgtgcggg cgaaattacc tggccggcac cgccgattca ggtatcagct
    21961 cagccgcagg cggcacaaaa agcggcaccg gaagtgaaaa ctgaggaaaa atgtgcctgc
    22021 tcaccgtggc gtaaatacgc gttgatggcg ctggcaatca tcctgttcgg ttggctgtca
    22081 agcgttgcgc cgaaagagtt tcttgggcac ttcaccgttt tcgcgctggc ctgcgttgtc
    22141 ggttattacg tggtgtggaa tgtatcgcac gcgctgcata caccgttgat gtcggttacc
    22201 aacgcgattt cagggattat tgttgtcgga gcactgttgc agattggcca gggaggctgg
    22261 gttagcttcc ttagttttat cgcggtgctt atagccagca ttaatatttt cggtggcttc
    22321 accgtgactc agcgcatgct gaaaatgttc cgcaaaaatt aaggggtaac atatgtctgg
    22381 aggattagtt acagctgcat acattgttgc cgcgatcctg tttatcttca gtctggccgg
    22441 tctttcgaaa catgaaacgt ctcgccaggg taacaacttc ggtatcgccg ggatggcgat
    22501 tgcgttaatc gcaaccattt ttggaccgga tacgggtaat gttggctgga tcttgctggc
    22561 gatggtcatt ggtggggcaa ttggtatccg gctggcgaag aaagttgaaa tgaccgaaat
    22621 gccagaactg gtggcgatcc tgcacagctt cgtgggtctg gcggcagtgc tggttggctt
    22681 taacagttat ctgcatcatg acgcgggaat ggcaccgatt ctggtcaata ttcacctgac
    22741 ggaagtgttc ctcggtatct tcatcggggc ggtaacgttc acgggctcgg tggtggcgtt
    22801 cggcaaactg tgtggcaaga tttcgtctaa accgttgatg ctgccaaacc gtcacaaaat
    22861 gaacctggcg gctctggtcg tttccttctt gctgctgatt gtatttgttc gcacggacag
    22921 cgtcggcctg caagtgctgg cattgctgat aatgaccgcc attgcgctgg tattcggctg
    22981 gcatttagtc gcctccatcg gtggtgcaga tatgccggtg gtggtgtcga tgctgaactc
    23041 gtactccggc tgggcggctg cggctgcggg ctttatgctc agcaacgacc tgctgattgt
    23101 gaccggtgcg ctggtcggtt cttcgggggc tatcctttct tacattatgt gtaaggcgat
    23161 gaaccgttcc tttatcagcg ttattgcggg tggtttcggc accgacggct cttctactgg
    23221 cgatgatcag gaagtgggtg agcaccgcga aatcaccgca gaagagacag cggaactgct
    23281 gaaaaactcc cattcagtga tcattactcc ggggtacggc atggcagtcg cgcaggcgca
    23341 atatcctgtc gctgaaatta ccgagaaact ccgcgctcgt ggtattaatg tgcgtttcgg
    23401 tatccacccg gttgcggggc gtttgcctgg acatatgaac gtattgctgg ctgaagcaaa
    23461 agtaccgtat gacatcgtgc tggaaatgga cgagatcaac gatgactttg ctgataccga
    23521 taccgtactg gtgattggtg ctaacgatac ggttaacccg gcggcgcagg atgatccgaa
    23581 gagtccgatt gctggtatgc ctgtgctgga agtgtggaaa gcgcagaacg tgattgtctt
    23641 taaacgttcg atgaacactg gctatgctgg tgtgcaaaac ccgctgttct tcaaggaaaa
    23701 cacccacatg ctgtttggtg acgccaaagc cagcgtggat gcaatcctga aagctctgta
    23761 accctgacgg cctctgcgga ggccgtcact ctttattgag atcgcttaac agaacggcga
    23821 tgctttgacc tcccgctgtt tgttcaagcg caattttgac aataattgtc aacggcacgg
    23881 aaagcagcat acccaccggt cctaacaacc atccccaaaa aatcaacgac aaaaatacca
    23941 ccaatgtcga aagccccagc ccacgtccca tgatgcgcgg ctccagaata ttgccgaaga
    24001 ccagattaat cagcagatat cccgccagca ccagcaacgc ttcgtagaag ccattaaaca
    24061 ccagtacctg agcgataggg gggattgccg cgagaactga accaatatta gggatgtaat
    24121 taagcgcaaa ggccagcaat ccccagacaa aagcgaagcg aacatcgagt gcggcgagca
    24181 tcgcccaggc gaccaggccg gtgatgatgc tgatggctgt tttcagcacc agataatgag
    24241 aaacactgtc aatcgcacgt tgaatcgccg ccatcccttc aaccggacgc gccatcattt
    24301 gctgaaattt tccgggcaat tgtggcactt cgagcagcat aaacagcacc gtcagcagca
    24361 ataaaaatat tgatgacatg gcattagata actgcgtcaa taagttggtg agcaacgtca
    24421 tcgccgcgtt cggatcaatg taatgcgcca gttggtcaac tgagacgtca atccctacgc
    24481 gttgcaacaa cggctcaaga gcttgcagcg gcgtcataat agagttgcga tattgcggta
    24541 acgtccgcgt caactcgttg agcgcggaac ccagataagc taatagcaac accatcgcca
    24601 tcacgatgat tgtcatcaaa atcgacactg ccagtacacg cggcacacgc cagcggacca
    24661 tatgttgcac cagcgggtta agaataacag caataaataa tgcgagaata aacggcacga
    24721 tgatctcggc ggcaaaacgg ataccgcaga gaataatgac cagcattccc aacataatga
    24781 cgatttttag gccattgagc gtgatgatcg gctttgccat gcttgtcccc gtttttcttc
    24841 ctttgctaat aataatgtct ttttcgctcc gtcatcataa cgaaaatcaa accgctcagg
    24901 taaagaagtg aaaatatttt gagttaattc ttaagctatg atacaaatca ggcgtgttca
    24961 actaccgagg acaattatca tctgcgatga cgagaagcaa cactgcggat aattgtaata
    25021 ttatggacaa tttgttcagg acgttatttt ctacatttac ccatcttcgt acctcatcca
    25081 ctatcttgct cgtaggtgag cagcactggc gcaacgcgct ttagtccttt cttctgccta
    25141 agcttgccct atgcgcaggg cttgaccact ttactttggt ttcgctgaat taagcgaaaa
    25201 ttaaaatagt tctcttgcag gagaaggaca atgtatattt attggatttt attaggtctg
    25261 gctattgcta cagaaattac cggtacgctg tcaatgaaat gggcgagcgt cagtgaggga
    25321 aatggcggct ttattttaat gctggtgatg atttctctgt cgtatatatt tctctctttc
    25381 gccgttaaaa aaatcgcctt aggcgtagct tatgcgctgt gggaaggtat cggtatttta
    25441 tttattacct tgtttagcgt tttgttattc gacgaaagtt tatcgctgat gaaaattgcc
    25501 gggttaacca ccctggtcgc cgggatagtg ttgatcaaat caggtacccg taaagcgcgt
    25561 aaacctgaac tggaggtgaa ccatggcgca gtttgaatgg gttcacgccg cctggctggc
    25621 attggcaatc gtgctggaaa tcgttgctaa cgtctttttg aaattttctg acggctttcg
    25681 tcgcaaaata tttggcttgc tctccctggc ggcggtgctg gcggccttta gtgcgctttc
    25741 tcaggccgtt aaagggatcg acttgtctgt cgtttatgca ttgtggggcg ggtttggtat
    25801 tgccgccacg ttagccgcag gttggatctt gtttggtcaa cggttaaatc gtaaaggctg
    25861 gattggcctg gtcttgctgt tggctggaat gatcatggtg aaacttgcct gatgaagacg
    25921 ctgcccgcgc tgtcgggcag cgtttgaaca ttatttttgc gacagttgat ccagcttgtc
    25981 gcgaaaaccg gtaacagaaa tggcccggtt atcggcgcgc cagcgatctt tcgcggcagg
    26041 agccgaactt tgcaccccaa ttaattgcca gccgtcatcg gtatgcaaca tcagaggcga
    26101 accgctgtca cccggcaagg tatcgcactg atgtgacatc accgacgttt gcgcccagcc
    26161 agtcacttca cagttttgat gactgtataa cgtatcgaga tgatcttcag ggtatcccgc
    26221 ctgagtcact ttacgaccag ccgattttaa tgcggcagta agcgcggctt tatctccctc
    26281 aaataacggc aacggtgtaa tgccagaagg gggattacgt agcacaatca atccgaagtc
    26341 ccacggtgca gctgcgggag gaacaatcca accatcccca tctgctttta accgctttcc
    26401 cagtgtcgga tcaacgcggc cttctatgtc gtggatctca tagcgccaaa gacctttatt
    26461 tgacacaaaa cgcagcgcta ccgccttatc cgctttaccc ttaggcggcg tcaataaaca
    26521 gtgtcctgcc gttaatgcca gattgggtgc aatcagcgtc gccgtacata aattgccgct
    26581 ggccgtttcc agttgcccaa ccgcatccca cggtgattgg gtcgtgtcat tcactggcac
    26641 acgatcatca tgaccaaaaa acagggtgct gacctcatcg tttgccgatt tggcaacgtc
    26701 tggtttatct gcaaacacaa aagcagacgt caaactaatt gcacccaaca ctacagcaat
    26761 ggttgtacgc atatcacact ctggtgggta attatgatta ttaaaagcaa accctcataa
    26821 atactataga cgggacggga tgaaagtggg agtaaaatca gataactaca gtcaggaaag
    26881 ataaaaacgg gcggcaatca gtgcgcataa aataaggatg ataagaatga actcaaagcg
    26941 ataacggccc accatactac cctccgctaa aggcggtgct aaacgttacc cgcttagcac
    27001 cgtaattttc aggcgcgagg gggcgcgcca gcattactgt tgaaaactta cgctgcgggt
    27061 tgtgcagcag gttttgccgg ttgctgatgg ccgtgtttcc cggcgtgttt cttcgctgcc
    27121 tgcgcttttt gttcaggggc tttctgttca gctttcgtat ttttatgatg ctttttagcc
    27181 gcctgcgctt tctgggcagg tgctgcttta tgctgttttt tatgatgtgt agttttcgct
    27241 ggcgctgctt tggtggtcgt cgcagtcgga gcaggtgtgg tcgcagtctc tgcagcaaag
    27301 gcggcagaag acagacccat agcagcggca acaaccagag ctaatacttt tttcattgtc
    27361 ataccctcaa tttgtttttt catttaaccc cactgcgggg ccgttgaaat aactatatcc
    27421 ctgggaatgg cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc
    27481 tgaataaatt acagcgttga taataacgta ttgtgactta agaaaaattt agctaccaat
    27541 aatagtagtc ttgatcgggt taactattat actacttaca aagcggtaga ataactgcgc
    27601 atcagtaata aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga
    27661 tgacgctaga tgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa
    27721 cgttggtttg agcaggcaat aagtccggac gggtatttac cgcagtccgg acttattttt
    27781 caggcgtgca gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga
    27841 gcaataaatg cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc
    27901 ccggcaatac tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta
    27961 gcgcgttttg cgcgggggcc gatccagctg ctggctactg agtgggctgc gaagaatccg
    28021 gctgagaaga gtaacattcc ggcaaagatc agccacagcg agctaaataa ggtcatcagt
    28081 aaaccaaacg gcataactcc cgtcgaaaac aacatcaccg gaccgcgccc atagcgggtg
    28141 gtcatggttc cggctttggg tgagctccat gtaccggtca aataagccag cgataataag
    28201 ccaaccacgg cctgactgac atgctagggg gaaagcatca accgatagcc gatgtaatta
    28261 aacagcgtga cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc
    28321 cggtcacgcc agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg
    28381 caaaaatggc gtgattcagg gaggattttc cagaacatca acgccgaggc cagcgcgaaa
    28441 caaccgattg ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt
    28501 aagcgtccgc tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc
    28561 acgaaactgg ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt
    28621 aacgaaagcc caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc
    28681 gaaagtaacg tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca
    28741 atggcatcgg atagcgggcc agtaaatagc aaaccgatag ccaacatcgc cgtggaaatg
    28801 gacagtgaaa tactactgtt cgcgggcgtt aagccaaact cctgcgaaag caccggaagg
    28861 ataggctgca cacaatagag aagtgcaaag gttgccagtc cggcagagaa cagcgccagg
    28921 gtgacgcgca taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt
    28981 tgcttgtcag tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat
    29041 ccttgctaaa tatgcctgtg gatcaggctt atacataggg taggaaaatc gaattgttct
    29101 gtctaatata ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat
    29161 ctgcgttact ttgttgctgt tgcggaagag ctgcattttg ggcgcgcggc tgcccgcctg
    29221 aatatttcgc aaccgccgct aagtcagcag attcaggcac tggagcaaca aatcggtgcc
    29281 cgactgctgg cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt
    29341 gcagatagtc ggcaaatcct gtctatggtg gatgacgctg ccgctcgcgc tgaaagactg
    29401 catcagggtg aagcggggga gttgcgtatt ggttttactt cgtccgctcc ttttattcgg
    29461 gcggtgtccg atacgttatc gctgtttcgc cgtgattatc ctgatgtcca tttacaaacc
    29521 cgcgaaatga acactcgcga gcaaatcgct ccgctcattg aaggaacgct ggatatggga
    29581 ttgctgcgta acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg
    29641 cttatggcga tgatcccgta cgatcatccc ctggcaaata atccgagtgt aacactggct
    29701 gaactggctg aactggctga actggcgaaa gaaccctttg tcttttttga tccgcacgtc
    29761 gggactgggc tgtatgacga tattctcgga ctgatgcgac gttaccattt gacgcccgtc
    29821 atcactcagg aggtgggcga ggcaatgacc atcatcggtc tggtttccgc cggtctgggt
    29881 gtttcaattt tgcctgcgtc atttaaacgt gttcagctca acgaaatgcg ctgggtgccg
    29941 attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca tcatgaacaa
    30001 agtccagctg cgcgtaactt tcgtattcat ctgctgaatg ctctcaggtg agggaaattt
    30061 cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgcc taagtaaaaa tttgacgaca
    30121 cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa tatagggagt
    30181 atgcggtggt tgctgaaaac cagcctgggc acattgatca aataaagcag accaacgcgg
    30241 gcgcggttta tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat ctttcccgtc
    30301 tggcgcagct ggctcctgcc agtatcacta aaattgtccg tgagatgctc gaagcacacc
    30361 tggtgcgaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg gtggggctgg
    30421 tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggg gagattttcc
    30481 ttgctctgcg cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa ctggcgttaa
    30541 aagatgactt gccattgctg gatcgtatca tttcccatat cgatcagttt tttatccgcc
    30601 accagaaaaa acttgagcgt ctaacttcga ttgccataac cctgccggga attattgata
    30661 cggaaaatgg tattgtacat cgcatgccgt tctacgagga tgtaaaagag atgccgctcg
    30721 gcgaggcgct ggagcagcat accggcgtac cggtttatat tcagcatgat atcagcgcat
    30781 ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg attcaggtgg
    30841 ttatcgatca caacgtgggg gtgggcgtca ttaccgatgg tcatctgcta cacgcaggca
    30901 gcagtagtct cgtggaaata ggccacacac aggtcgaccc atatggtaaa cgctgttatt
    30961 gcgggaacca cggctgcctc gaaaccatcg ccagtgtgga cagtattctt gagctggcac
    31021 agctgcgtct caatcaatcc atgagctcga tgttacatgg gcaaccgtta accgtggact
    31081 cattgtgtca ggcggcattg cgcggcgatc tactggcaaa agacatcatt atcggggtgg
    31141 gcgcgcatgt agggcgcatt cttgccatca tggtgaattt atttaaccca caaaaaatac
    31201 tgattggctc accgttaagt aaagcggcag atatcctctt cccggtcatc tcagacagca
    31261 tccgtcagca ggcccttcct gcgtatagtc agcacattag cgttgagagt actcagtttt
    31321 ctaaccaggg cacgatggca ggcgctgcac tggtaaaaga cgcgatgtat aacggttctt
    31381 tgttgattcg tctgttgcag ggttaacatt ttttaactgt tctaccaaaa tttgcgctat
    31441 ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt tcctagttta
    31501 tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat taccggtaca
    31561 gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt agcctcccag
    31621 ggaaaaaggg ttgcgggata taaacccgta gcgaagggga gcaaagagac acccgaaggg
    31681 ctgcgtaata aagatgcctt ggtgttgcag agtgtttcaa ccatcgaact gccttatgaa
    31741 gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag ttgcccaatc
    31801 aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga tcatgtcgtg
    31861 gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccact ctctgaatgg
    31921 gtagtgcagg aacaactgcc ggtgttgatg gttgtcggta ttcaggaagg ttgcattaac
    31981 catgcactgc taacagctca ggcgatcgcc aacgacgggc tgccgctcat tggctgggtg
    32041 gctaaccgaa tcaacccagg actggcgcat tatgcggaaa tcattgatgt gctgggtaaa
    32101 aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga acagcgtgaa
    32161 ctggggcaat acatccgctt agctatgctg cgcagtgtgc tggcggtaga tagagtcacg
    32221 gtgtaacgtc cgcgaaatta ccgacgcaat tacgcaggca atcaataaac cggggagtag
    32281 ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg cgtgcgtggt
    32341 tgccgccaac agtgtcgcca tcccggtcaa tccgagtaaa agcgtaattt cttcgccatc
    32401 agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac cgataaatag
    32461 cgtcggtgta aagacaccac cgggtgcgcc ggaaccgcta ctcgccagca cggcacacag
    32521 tttacaaagg aagatcccgg caatgatcat taacagtggt ggggcggtta aaaaggattg
    32581 tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca gacccacgat
    32641 caacccgcct agtgccagtt gccagggcgg cgcaagtttg agactcacaa atccacgatg
    32701 acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca cacctgtact
    32761 gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgt tgtagagtaa
    32821 cgcgtcgcta tgattaatca gattgctaac cagccatgcc acgatggcgg aaataatcac
    32881 cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa ataaactgcc
    32941 agcaagcggg gcacgatagg ccgcagccat tcccgccgcg gccccacagg cgatccataa
    33001 tttccattcc tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa gggcagctaa
    33061 aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca gcagcgaggc
    33121 aagcgattta accaggcttg ctgcgtaatc gaactgtcca tcggtttgca acgcttccat
    33181 gtaatcggtc ggcgcatgag ggcgttgttg ggtaaatttc tgccagccca tcagcaacaa
    33241 acccgccgcc agtccgccga gcgccggagt tagcagccgt cgccaggagg aaaggtttgt
    33301 cgctgcattg accagactgc cggagtcatt attgaggaac aaccactcca gtagcagcat
    33361 cgcatgacga aacccggcaa cggcaaagac agcgagaata ccgacgactg ttgcgataag
    33421 cagacggcgg aacatagtgc gcagatccgg atagatgtgt agatgatgca tgagcagaaa
    33481 tgggcaatga aacacgggcg actattttgc ggcaaaagag gccaaccgca aaagcagtgc
    33541 gtgctttaag agataaaaaa acgtgacatt gtaacgtatt attgccgaat cgtgcgtaat
    33601 cgtcaccatc cggcaatatt acggtgatcc tatcgaaaga gtggtttaac cgcgacagga
    33661 atcaacagtt gtgattgcca ttgcgccagc gttaagcgag ccagttcacc cagtgcacgg
    33721 tagaaagggt gttctgcttt ttcgataaaa acatcaagaa aacgtgttga ccacggaaaa
    33781 aggtgccatg ccagcaattc ttcgcattct gtctggcgac cattctccgc caaccacgca
    33841 gccatcaaca gcagtgaacc aaaatgatct tccggttcgt tttgcttcat ttcaaactga
    33901 atgcctttct cgcgcatcca ctgacgaagt gccaatgttg aatcgccaaa cagcacagat
    33961 tcgcgatcca gccagaccga cccccacggc ggagacggca gtgcccacgg gccgacaaac
    34021 aaacgctgcc aggcctgggc gtgagtctct tcacactgtg tctgaaaagc cgtcaccagc
    34081 ggcgctaatg acgcttctgg tagaggccac tgagtttccc agccgtcact ggtgagtacc
    34141 gcaaccagag gtgcggcttc cgcactctct ggagcgtaat aaaacagcgc gcccaacacg
    34201 cgcgccgcga cagaaaaatt atcttgctgt gaaaaatggg tcattcctaa catcctgaaa
    34261 tgtgcgggtt gccccgcacc aatttgttaa cctgcaatag ccattcctac tgtcatgtgc
    34321 aggccataaa agagcacacg cccgatcatc tcaccaacaa gtatgaggat tagccccaga
    34381 acaagtccgg caacgtgagg ttcacggcga cgaatgagcg gacaaagcca gcatccaaga
    34441 cctgcgcata acaataccac acgccagacc tgtaaggagg cgtaatctgg taccagtgcg
    34501 ctggcttgtt gcacggatga gtgaatagac gccagagaca aaccttgcag cacaatcacc
    34561 tcggcacaag cgatcagcgc cagaacgcta atgatggcaa atggtgtggt attaaatgta
    34621 acgcgggctg cccgcagaat tgcggcagca agtattggac cgctcaacaa taccgtcagg
    34681 aagaaagcca gcgtagtgta accgttatgc caggttggca cggtgtcgat ttgatacacg
    34741 caggtcatca tccagacgaa aatgacgccg agcgccatgc tgacaagtaa ccagagtttc
    34801 cccaacgctt gcggcatttt accgatgacc gccaccagcc accatagtcc gccaacggca
    34861 aaaaatatgg aaccggcagc aatctcgtta ctcagaccag aagctccgat tcgattcagc
    34921 gagttaaacg ctcgcagtgg cgatcccagg tgcatgacag aggcaataaa cccgatgccc
    34981 atcaacagcc agaggaaaaa catgccacga acaatgtgct gacggtctgc gtcatttttg
    35041 gcggcgaacc agccaattcc gctaacaatc agcgcgccta ctacgcattg acccaatacg
    35101 gtaaagataa ccagtggcca ttcatgccat ccatttccca ttttacactt cctccggatt
    35161 agccagataa ccagtggtat cgccggtcgg gcggctgttg gcgttaggtt tgataacgat
    35221 attgggtttt gtgaaatgcg cgcgcggcag tggcgcgacg gcagccagag tgccgtgctt
    35281 ctggcgcagc tcttcaattg gaccgaactc aagtgcacgc agcgggcagg attcgacgca
    35341 tatgggttgt ttcccctcgg cgacgcgcga ataacaacca tcgcacttcg tcatgtgccc
    35401 tttttcagca ttgtactgtg gtgcgccgta cggacaagcc atgtggcagt agcggcagcc
    35461 gatacaaaca tcttcatcaa ccaccacaaa gccatcttca cgcttatgca ttgcgccgct
    35521 ggggcagact tttgtacatg cggggtcatc gcaatggtta caggagatgg agagataata
    35581 agcgaacacg ttctggtgcc agatgccatt atcttcctgc cagtcgccgc cagcatattc
    35641 ataaatacgg cggaaactga cttccgggcc aagatcttta aaatctttgc acgccagttc
    35701 gcaggtttta cagccagtac agcggctgga atcgataaaa aatccatatt gtgtggtcat
    35761 gggctactcc ttaaaccttt tcgatctgga caagattgct gtgcgacggg tttccctttg
    35821 ccagcggtga agggcggtga gaggtcagaa tattgatact gccgccgtga tcgacccggt
    35881 caccaaacat atccgcttta agccacgcac cttgcccgat ggcggtaacg ccaggcagaa
    35941 tacgcggagt cacttttgcg gcaatcagca tttctccatt attgttaaat acccgcacgg
    36001 tatcgccatg acggataccg cgtgcctgag catcaatggg gttgatccac acctcttgtg
    36061 ggcaggcctg ctgtaacaca tcaatattgc cgtagctgga gtgggtacgc gctttggttt
    36121 ggcgacttca ctcagcatcc agtagatggg tttgcgttca aattttgctg aggttgcggg
    36181 ttgggcgagg atcacatagc ccatattccc tgcagattca tgagagataa ggtcttcttg
    36241 ctctgttggc atcaggtcag gcaacaggat atcgcaatac ttagccgagg ccgtcatgaa
    36301 gtggtcaatg ccaacaatca tctcgcactt gctgtcatcc tgaagcacct catgggtgtg
    36361 attgatgtcg ccatgttgat tgatcaatgt gttactggcg tagcaccata aaaacttgat
    36421 ggggacatcc agtttttctt ttccacgaac accatcacgg gtcgcggtca ttttcgtacc
    36481 atggtcgatg gcatctgtcc atgtaaaaac ggaaatctgc gttttaacag gattctcgag
    36541 catcgggaac cattctaccc ccagatccca gctaccttcg cgtacacctg agttgccgcc
    36601 gtttatgccg acgttaccgg taagaacgga aagcatggca atagcgcggg acgtttgttc
    36661 gccgttggag tgtcgttgtg gcccccaacc ctgacaaata taagcaggtt ttgctgaacc
    36721 gatctctcgt gccaactgga taattttttc tgccgggatg ctggtgattt ttgctgccca
    36781 ttccggcgtt ttagctatgc cgtcaggccc ttcgcccaga atataggctt tataatgcgc
    36841 gttacgtggt gcgttggcgg gcagcgtttt ttcatcgtaa ccaacacaat atttgtcgag
    36901 aaatggctga tcgaccatgt tttcagtaat cagtacccag gcaatcgcac aggccagtgc
    36961 gccatcggtg ccagggcgaa tgggcagcca ttcatcttca cgcccggcag ctgtgtcgtt
    37021 atagcgtgga tcgatgacga tcatgcgtgc gtttgaacgt tcgcgggctt gctcgacgta
    37081 gtaagtgaca ccaccgccgc tcatccgcgt ttctgccggg ttatttccga acataacgac
    37141 cagtttcgta ttggcgatat catccgggct gttgccatca ttggcaccga acatataact
    37201 cattgcggca ctgatctgtg cggtactgta gctgccatag cgactgagaa aacccccgca
    37261 agagttcatc agacggtacg ggacgttcga gttggtgata tttccgccat ctacgcctgt
    37321 tccgtacagg acatgtacag cctcattgcc gtaatctttc aggatccgcc gaagattatc
    37381 actgatggta tccagggctt cgtcccaact tatccgttca aatttacctt caccgcgctc
    37441 gccgacgcgc ttcatgggat atttcaacct gtcaggatga ttcatccgtc ggcggataga
    37501 gcgcccgcgt aaacatgccc gaacctgatg attaccgtaa acgtcgtcac ctgtcgtatc
    37561 agactccacc cagtacacgg tgtcatcttt cacatgcaaa cgtagcagac agcggctccc
    37621 gcagttaacg gtgcaggaac tccagaccgt tttctcttct accggagcct ctgccgcccg
    37681 gaccatttgg gaaaatggca gagtgaaagc actgcttgcc agcgcgagac tgccaagtgc
    37741 ggaggttttc atcagacttc tatggctgat ttcagccttc atgagcgcct ctgtggtatg
    37801 gattttcatc attactcact tattgctttt caaacaaaat gtcatgccag aatttatggt
    37861 tgtagtgggt tatatttttt cgatctcgac cagattagtg tgctgcgggt ttcccttcgc
    37921 cagtggtgaa gggcgcagag tggttagcgt attcacacat ccgccatggt cgattttatc
    37981 gccagacata ttgggctcgt gccaggctcc ctggcccata gcgctaactc cagggagaat
    38041 gcgtggtgtt actttggctg gtagccgaac ttcgccacga tggttaaaca cccgcaccat
    38101 atcgccgttg gcaatcccac gtttctgtgc atctataggg ttgatccaca cctcctgacg
    38161 gcaggctgcc ttcaggacat caatgttgcc gtaggtcgag tgagtacggg atttgtaatg
    38221 gaaaccaaac agttgcagtg ggaaggttct acgttcaggg gagtcccagc cttcaaaggt
    38281 tgaggcataa actggcagtg ggcttatcac ttcatctttt tccagttccc aggtacgggc
    38341 aatttccgcc agcttgctgg aataaatttc aatcttaccg gaaggcgttt taagtggatt
    38401 tgcctcgggg tcgtcacgaa atgctttgta ggcgacaaaa tggccattgg gatctttacg
    38461 cttatagata cccatttttt tcagttcgtc gtaagacggt aacgccggat ctttggcaag
    38521 cattttggcg tacagatgtt gtaaccattg ttcctgcgtg cgaccttctg tgaacttttg
    38581 atagacgtca ggtccaagac gtttcgcgac ttcactcagg atccagtaaa tcggtttacg
    38641 ttcgaatttt tcgctggtga ctggctggag gaaaatgaga tatcccatgt taccggcgta
    38701 gtcgttagga ataatatctt cctgctcaac ggtcatcagg tcttccagca gaatgtcggc
    38761 atattttgcc gatgaggtca taaagttttc gatgaccaca atcatttcgc atttcgattc
    38821 gtcctgcaga atttcatgcg ttttgttgat gtcagaatgc tgattaacga gggtatttcc
    38881 cgcgtagttc cagatggact ccattgccag acggatggat ggacttgaag tagttgactc
    38941 aatgctactt aaggtcctga tatgactgac attaatcatg ataacactgt atatccatcc
    39001 agttctttct ctgggtctcc cta
//
LOCUS       DAJTRM010000027        38616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRM010000027 DAJTRM010000000
VERSION     DAJTRM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.027"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..77)
                     /locus_tag="OO322_001518"
     CDS             complement(<1..77)
                     /locus_tag="OO322_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Antitoxin that counteracts the effect of the YoeB
                     toxin; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCR5906447.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAP"
     gene            360..401
                     /gene="hisL"
                     /locus_tag="OO322_001519"
     CDS             360..401
                     /gene="hisL"
                     /locus_tag="OO322_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5906448.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    377..500
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            547..1446
                     /gene="hisG"
                     /locus_tag="OO322_001520"
     CDS             547..1446
                     /gene="hisG"
                     /locus_tag="OO322_001520"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5906449.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1452..2756
                     /gene="hisD"
                     /locus_tag="OO322_001521"
     CDS             1452..2756
                     /gene="hisD"
                     /locus_tag="OO322_001521"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5906450.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2753..3823
                     /gene="hisC"
                     /locus_tag="OO322_001522"
     CDS             2753..3823
                     /gene="hisC"
                     /locus_tag="OO322_001522"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5906451.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3823..4890
                     /gene="hisB"
                     /locus_tag="OO322_001523"
     CDS             3823..4890
                     /gene="hisB"
                     /locus_tag="OO322_001523"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5906452.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4890..5480
                     /gene="hisH"
                     /locus_tag="OO322_001524"
     CDS             4890..5480
                     /gene="hisH"
                     /locus_tag="OO322_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5906453.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5480..6217
                     /gene="hisA"
                     /locus_tag="OO322_001525"
     CDS             5480..6217
                     /gene="hisA"
                     /locus_tag="OO322_001525"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5906454.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6199..6975
                     /gene="hisF"
                     /locus_tag="OO322_001526"
     CDS             6199..6975
                     /gene="hisF"
                     /locus_tag="OO322_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5906455.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6969..7580
                     /locus_tag="OO322_001527"
     CDS             6969..7580
                     /locus_tag="OO322_001527"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5906456.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7766..8743)
                     /gene="wzzB"
                     /locus_tag="OO322_001528"
     CDS             complement(7766..8743)
                     /gene="wzzB"
                     /locus_tag="OO322_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5906457.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8889..10009)
                     /gene="ugd"
                     /locus_tag="OO322_001529"
                     /pseudo
     CDS             complement(8889..10009)
                     /gene="ugd"
                     /locus_tag="OO322_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10259..11665)
                     /gene="gndA"
                     /locus_tag="OO322_001530"
     CDS             complement(10259..11665)
                     /gene="gndA"
                     /locus_tag="OO322_001530"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5906458.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11774..12133)
                     /locus_tag="OO322_001531"
     CDS             complement(11774..12133)
                     /locus_tag="OO322_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906459.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12130..12636)
                     /locus_tag="OO322_001532"
     CDS             complement(12130..12636)
                     /locus_tag="OO322_001532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906460.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12782..13345)
                     /locus_tag="OO322_001533"
     CDS             complement(12782..13345)
                     /locus_tag="OO322_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906461.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13552..14490)
                     /locus_tag="OO322_001534"
                     /pseudo
     CDS             complement(13552..14490)
                     /locus_tag="OO322_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14553..14927)
                     /locus_tag="OO322_001535"
     CDS             complement(14553..14927)
                     /locus_tag="OO322_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5906462.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14931..16079)
                     /gene="wzy"
                     /locus_tag="OO322_001536"
     CDS             complement(14931..16079)
                     /gene="wzy"
                     /locus_tag="OO322_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5906463.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16099..17004)
                     /gene="rfbG"
                     /locus_tag="OO322_001537"
     CDS             complement(16099..17004)
                     /gene="rfbG"
                     /locus_tag="OO322_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5906464.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16997..17887)
                     /locus_tag="OO322_001538"
     CDS             complement(16997..17887)
                     /locus_tag="OO322_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5906465.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17917..19173)
                     /gene="wzx"
                     /locus_tag="OO322_001539"
     CDS             complement(17917..19173)
                     /gene="wzx"
                     /locus_tag="OO322_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5906466.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19189..19734)
                     /gene="rfbC"
                     /locus_tag="OO322_001540"
     CDS             complement(19189..19734)
                     /gene="rfbC"
                     /locus_tag="OO322_001540"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5906467.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19739..20617)
                     /gene="rfbA"
                     /locus_tag="OO322_001541"
     CDS             complement(19739..20617)
                     /gene="rfbA"
                     /locus_tag="OO322_001541"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5906468.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20676..21575)
                     /gene="rfbD"
                     /locus_tag="OO322_001542"
     CDS             complement(20676..21575)
                     /gene="rfbD"
                     /locus_tag="OO322_001542"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5906469.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21575..22660)
                     /gene="rfbB"
                     /locus_tag="OO322_001543"
     CDS             complement(21575..22660)
                     /gene="rfbB"
                     /locus_tag="OO322_001543"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5906470.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23033..23926)
                     /gene="galF"
                     /locus_tag="OO322_001544"
     CDS             complement(23033..23926)
                     /gene="galF"
                     /locus_tag="OO322_001544"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5906471.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24101..25495)
                     /gene="wcaM"
                     /locus_tag="OO322_001545"
     CDS             complement(24101..25495)
                     /gene="wcaM"
                     /locus_tag="OO322_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5906472.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25506..26726)
                     /gene="wcaL"
                     /locus_tag="OO322_001546"
     CDS             complement(25506..26726)
                     /gene="wcaL"
                     /locus_tag="OO322_001546"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5906473.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26723..28003)
                     /gene="wcaK"
                     /locus_tag="OO322_001547"
     CDS             complement(26723..28003)
                     /gene="wcaK"
                     /locus_tag="OO322_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5906474.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28183..29661)
                     /gene="wzxC"
                     /locus_tag="OO322_001548"
     CDS             complement(28183..29661)
                     /gene="wzxC"
                     /locus_tag="OO322_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5906475.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29663..31057)
                     /gene="wcaJ"
                     /locus_tag="OO322_001549"
     CDS             complement(29663..31057)
                     /gene="wcaJ"
                     /locus_tag="OO322_001549"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5906476.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31112..32482)
                     /gene="cpsG"
                     /locus_tag="OO322_001550"
                     /gene_synonym="manB"
     CDS             complement(31112..32482)
                     /gene="cpsG"
                     /locus_tag="OO322_001550"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5906477.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32675..34111)
                     /gene="cpsB"
                     /locus_tag="OO322_001551"
     CDS             complement(32675..34111)
                     /gene="cpsB"
                     /locus_tag="OO322_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5906478.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34114..35337)
                     /gene="wcaI"
                     /locus_tag="OO322_001552"
     CDS             complement(34114..35337)
                     /gene="wcaI"
                     /locus_tag="OO322_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5906479.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35334..35813)
                     /gene="gmm"
                     /locus_tag="OO322_001553"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35334..35813)
                     /gene="gmm"
                     /locus_tag="OO322_001553"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5906480.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35813..36781)
                     /gene="fcl"
                     /locus_tag="OO322_001554"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35813..36781)
                     /gene="fcl"
                     /locus_tag="OO322_001554"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5906481.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36784..37905)
                     /gene="gmd"
                     /locus_tag="OO322_001555"
     CDS             complement(36784..37905)
                     /gene="gmd"
                     /locus_tag="OO322_001555"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5906482.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37931..38479)
                     /gene="wcaF"
                     /locus_tag="OO322_001556"
     CDS             complement(37931..38479)
                     /gene="wcaF"
                     /locus_tag="OO322_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5906483.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38495..>38616)
                     /locus_tag="OO322_001557"
     CDS             complement(38495..>38616)
                     /locus_tag="OO322_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5906484.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10314 a         9671 c         8691 g         9940 t
ORIGIN      
        1 ggggcatgat cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg
       61 tagctaattg tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg
      121 ttaattaact atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc
      181 agactttgct ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc
      241 cccgctcatt cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct
      301 aacgtgaaag tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta
      361 tgacacgcgt tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc
      421 tggaagacat tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca
      481 ccttccgggg gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat
      541 aagaaaatga cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt
      601 gatgactcac gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc
      661 ctgatcgcga tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt
      721 ccctgtctgg taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa
      781 gaagagctgc ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt
      841 ctggatttcg gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt
      901 ccgctctcct taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat
      961 ctcgaccaga aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg
     1021 ccgcgtgccg ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa
     1081 gctaacggcc tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc
     1141 gatggcgaaa tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag
     1201 ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg
     1261 gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt
     1321 gatcaacagc gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg
     1381 gaaaaactga aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg
     1441 gagtgatcgc catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac
     1501 aacgccagct gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta
     1561 acgatattct cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga
     1621 agtttgataa aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca
     1681 gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa
     1741 ccttccacac tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt
     1801 gccagcaggt cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc
     1861 cgctcttctc aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag
     1921 tggtgctgtg ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg
     1981 atgtgcagga cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta
     2041 cggaatctgt gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag
     2101 caaaacgcca ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt
     2161 cggaagtgct ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc
     2221 tttctcaggc tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata
     2281 tggcgcgtcg cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa
     2341 ccacccgcca ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg
     2401 tggagatctc caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg
     2461 aactggtcga tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat
     2521 cggcaggcga ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca
     2581 cctgttccag cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga
     2641 aagaaggttt ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga
     2701 ccgcccacaa aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac
     2761 cgtgactatt actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc
     2821 gcgtcgtctg ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt
     2881 ggagtttaag cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt
     2941 gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg
     3001 cgcggacgaa ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat
     3061 cctctactgc ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga
     3121 atgccgcaca gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa
     3181 gctggacggc gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat
     3241 caatccgcag gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc
     3301 cgatgaagct tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata
     3361 tccgcacctg gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg
     3421 cggatttacg ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta
     3481 cccgctctcg acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt
     3541 cgccatgagc gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact
     3601 gaaagagatc ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg
     3661 ctttaaagcc tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga
     3721 tcagaataaa caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga
     3781 aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt
     3841 tttatcgatc gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt
     3901 tttgacaaac tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg
     3961 ggctacaagc tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa
     4021 gcggattttg atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag
     4081 tttgatgaag tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg
     4141 aaagtaaaac tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat
     4201 gtaattggcg atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta
     4261 cgctacgacc gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac
     4321 cgttacgccc atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg
     4381 gatcgcgaag gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat
     4441 cagatcgcca cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc
     4501 gacgatcacc acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc
     4561 ctcggcgaca aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt
     4621 gcccgctgcg cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc
     4681 taccagcgtg tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct
     4741 tacaccatgg gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta
     4801 gaaagcctgt tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac
     4861 accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg
     4921 ccaacctgaa ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc
     4981 gtgacccgga cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac
     5041 aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc
     5101 aaccggtgct gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca
     5161 atggcgtcga cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc
     5221 tgccactgcc acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc
     5281 aggggattga agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc
     5341 cgtggaccat cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata
     5401 acttctacgg cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga
     5461 aaaacttcct ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt
     5521 gcgtctccat cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg
     5581 cttacaggat tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc
     5641 aaaagatccg gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt
     5701 cccggtgcag gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc
     5761 tggcgttgcg cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg
     5821 ctggtttgaa cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga
     5881 gcaaggcaac aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga
     5941 acaactggtg gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc
     6001 gcgcgacggc acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata
     6061 tccgcaagtg gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct
     6121 acgtggcact ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac
     6181 cgtgaaggag gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat
     6241 ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca
     6301 ctggcaaaac gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct
     6361 tccagcgatg gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac
     6421 attccgtttt gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc
     6481 tttggcgcgg ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact
     6541 cgcctggccg atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac
     6601 gccgaaaccg gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc
     6661 actcaatggg aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc
     6721 gtcctcaaca tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa
     6781 aaagtgcgtg aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa
     6841 cacttcctgg aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc
     6901 cacaaacaaa taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag
     6961 atcaggatat gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc
     7021 tgatgccggt gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga
     7081 acccggaagc cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta
     7141 aacagcgact gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg
     7201 ccccggactg cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc
     7261 acaaaggcac cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac
     7321 tggaacaact gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac
     7381 tgtatgccag cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg
     7441 cactggcagc aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt
     7501 atcacctgct ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc
     7561 tgcataaacg gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg
     7621 agccattgcc cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg
     7681 gataagatgc gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc
     7741 cgtttctctt aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc
     7801 ccaacacaat ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg
     7861 cctttttcgg actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat
     7921 gaatatcaag atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat
     7981 agtagtttgg tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg
     8041 cttcgctccc tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg
     8101 gctttgtcac ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca
     8161 gatctttctg ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc
     8221 gtcccagagc aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa
     8281 cttgctgaat gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag
     8341 aaacagtcaa tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct
     8401 cttcctgatt atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta
     8461 acgtctcctg caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat
     8521 tgttatagcc agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt
     8581 tctccttcgc tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga
     8641 taattgtcat cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg
     8701 ggtcatggtt ttgcccagaa acattattat tttctactct catcattacc ctaactgacg
     8761 gaaatttcca agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat
     8821 aaaaataatg aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata
     8881 acaagatgtt agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta
     8941 agctcttctg ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga
     9001 tcacgttcca ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc
     9061 acttcaacgc ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag
     9121 ttatctgacc cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga
     9181 caattgccga gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat
     9241 ctttcggcag acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac
     9301 gcggatcgag acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat
     9361 aactatccag ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt
     9421 taatcgcttc tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc
     9481 cttcctgtaa caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac
     9541 gtgacggatg gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa
     9601 taatattttc agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg
     9661 atttgatgac cataaccgca taaggattta tctcaactac gtctttaatt actgattcta
     9721 cactggatgt attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga
     9781 cataatcagc atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat
     9841 ctgattgcaa aaactgctga atttccttat caacaatagg agatatccga tcattcagca
     9901 tagcaacgcg tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa
     9961 gcccgtttga caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc
    10021 agaattaact taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt
    10081 taacgctacg ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga
    10141 atagctgatg tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc
    10201 aaaaaaaagc ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt
    10261 aatccagcca ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc
    10321 cgaaatagtc acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat
    10381 cgtaataggc aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag
    10441 cgacgacatc gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca
    10501 gcagcaggtt agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact
    10561 gcgcacggat gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc
    10621 aattgtattc ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt
    10681 tgcccagata cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact
    10741 gagcttgcgg accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga
    10801 tataacgtgc aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc
    10861 tctggctggt ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca
    10921 ggtagttacc gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc
    10981 tgctaagttc accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat
    11041 tcaggccacc tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa
    11101 taccattgtg aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc
    11161 atggctcgcc gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat
    11221 aagcctcttt ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc
    11281 cggaaacacc ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa
    11341 tggtgtcctg gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg
    11401 gtttcaggga atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc
    11461 gacgaggcgt ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct
    11521 tgcctggatt ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga
    11581 cggtataacc acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga
    11641 ctacgccgat ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta
    11701 ttaaccgtta tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt
    11761 tacagcatgc ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt
    11821 tgaattttgg atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa
    11881 acgaactatt aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact
    11941 cgggattact aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca
    12001 tccctttata gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata
    12061 actcactata ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg
    12121 aattaaaatt caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg
    12181 aaaaaaaata atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat
    12241 atcttttatt aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg
    12301 tgataaatac ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat
    12361 cgagaaaaat acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata
    12421 aaaaacaaca tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact
    12481 tataccatgt cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta
    12541 gtgaccaaat atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg
    12601 gacataccaa taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat
    12661 gtttctcttt ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag
    12721 ttttacgcca caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt
    12781 tttattgttt tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg
    12841 ttaaatcaat atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga
    12901 ttgtagcaat aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc
    12961 cacaggctat tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta
    13021 actgtaaacc taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat
    13081 attgttcaac aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca
    13141 tagtatcatc ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca
    13201 aaacaagaaa taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc
    13261 taaattgctg acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag
    13321 caatcttatt aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca
    13381 tccctatgca taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt
    13441 aaaaaaagat gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca
    13501 atacacaaca tacaaataca acggcaattc aatcatagta aaatactcta tttacattat
    13561 cagtttatag tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt
    13621 atttgaatgc aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc
    13681 agttaaaaag gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat
    13741 gggcaatcct aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta
    13801 tagtggccgt tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact
    13861 ccaagttgat agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc
    13921 accatacttc atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc
    13981 ccttgataga catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa
    14041 catatcagta aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc
    14101 tcagcgatgc ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac
    14161 catatccaat tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata
    14221 agtgtcatct ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct
    14281 tacgacattc cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc
    14341 cacttgtgct gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat
    14401 attcgttttg aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat
    14461 aattacagcg atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg
    14521 tgcaagtaat tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg
    14581 caatatccta aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa
    14641 gctaagagat caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat
    14701 ataaaatatc tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa
    14761 gaaaaggttg ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa
    14821 ttattataac acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa
    14881 aaataactaa aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct
    14941 ccagaagtga ggttattact aatttggata ttttctatag aaaataccca taaaactaca
    15001 acgcctataa taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta
    15061 atactgaaaa aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag
    15121 cctttcaaaa agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt
    15181 ttctgaagta tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca
    15241 aatcctcttc caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca
    15301 ttaaatctaa cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata
    15361 aatagaacgc tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa
    15421 ctggctaata acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat
    15481 atcgcaagca ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca
    15541 ccgacaccaa aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac
    15601 tcgctagtca atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac
    15661 aaaattatgt atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta
    15721 ataaaaatct cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt
    15781 gacagcataa aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct
    15841 aataagccga caaagaagcc ataaaaagga aataaaagca accatataaa aagactgaca
    15901 catttttggg tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta
    15961 aatacaaata ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca
    16021 ccaccaataa tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata
    16081 ttcgtaaggt gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt
    16141 atcagccttt ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata
    16201 acctttaccc aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt
    16261 tacttctttt cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa
    16321 actaatgttt gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct
    16381 atcaatatac cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag
    16441 acagatatct tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt
    16501 ttcttttttt ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg
    16561 agtttgcgac tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac
    16621 attgctggaa taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt
    16681 atctttatac caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc
    16741 gacatcttta attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg
    16801 aagtatattt ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt
    16861 atcagttgat aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat
    16921 actttttaat tgttcctcaa tccagcattc gccattatac gtagccataa gtacagctac
    16981 tcttttttga tcagaactat gcattataac gaccgccccc agtaattcct cttattccat
    17041 ccagcgtagc caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa
    17101 ttaaagtcgt atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat
    17161 gattttttct aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt
    17221 gaattggtat gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt
    17281 ctatatcagg aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg
    17341 tatcgacata atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt
    17401 ttgaaaccat ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag
    17461 tgacatgaat tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg
    17521 acttatctct atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc
    17581 aggaaagttt tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct
    17641 tctctgcccc tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt
    17701 cattcctacc aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg
    17761 tgaaattagt cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga
    17821 tcagtgcatt cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac
    17881 tattcattat ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat
    17941 tctagtataa agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat
    18001 tgcggcaccc agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac
    18061 caattcagca caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga
    18121 gaatggtatc attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt
    18181 aaaggaatta atttgataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa
    18241 caagcctatg attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac
    18301 attacgttta aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc
    18361 gattgctgca ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc
    18421 aactttatct gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg
    18481 acttatgata ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt
    18541 ataacaaaaa tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa
    18601 aatcgaaata actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata
    18661 aaaaacaaat atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt
    18721 tacaatgcca aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa
    18781 taatggaatg caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc
    18841 agaaattttt agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa
    18901 gcatgaaaat agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg
    18961 attatcacga tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat
    19021 tccagcatac ccaacaagtg caattgcaat tgtataaaca ccgaaacctt caggccccaa
    19081 acctcgagcg agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa
    19141 aaggttccag acactatttt ttattatgct cattattaca tttctttatc atataaaata
    19201 atcagcatct acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac
    19261 cggccattct atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga
    19321 atagaaatta tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg
    19381 agcgaatcct tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac
    19441 ccacttgcca aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc
    19501 gacaacacag cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg
    19561 taacacaccc ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac
    19621 agcctcttcg aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa
    19681 tacttttggt tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt
    19741 aataaccttt aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg
    19801 ctaatacctt cacttgctca gcatcaataa agcctttacg gtgagcaatt tcttccgggc
    19861 aggaaacttt cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa
    19921 gactttgatg tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca
    19981 aacgtccttg gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag
    20041 atggtttaag gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa
    20101 ccgcatagtt actttttggt tcctgcggtt tttcttccag actaattgcc gtaccgttat
    20161 catcaaattc caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg
    20221 cgccactctc tctgttaaca gcggtatcca ttaatttcgg caggtcgtga ccatagaaga
    20281 tattatcacc aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa
    20341 atgcttgcgc cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact
    20401 ggcttccatc accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca
    20461 gaatatctcg aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat
    20521 cataaatcgg tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg
    20581 aaccaccagc cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc
    20641 tcgctctagc atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat
    20701 tcgttgagca tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc
    20761 tgaaattttt cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc
    20821 gttgtcggca cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct
    20881 tcaaatacca gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac
    20941 aaaccagcaa cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc
    21001 agtaattcag cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt
    21061 tcttttgcca ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag
    21121 ctggtacgga aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc
    21181 ttggtttcac cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca
    21241 ccgttgcctg ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta
    21301 gctgcttttg caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct
    21361 gattccgctt tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt
    21421 tttttgacgg tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag
    21481 tgaacatcga gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc
    21541 acctgccctg ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt
    21601 caatccacga ctgataagta ccgcttttca cattctcaac ccaatttgta ttagccagat
    21661 accattccac cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt
    21721 cgcggctaat tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa
    21781 cataagtaat ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac
    21841 aaatagtgag cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt
    21901 cacccgcttt accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca
    21961 accagtcgcg aatttgatcc cctttaccat aaataggtaa tgccttacct tccagagcat
    22021 tcagaataac caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc
    22081 aattagtcac aatggttggt aaaccataag tccgcttcca ggcacgtacc aaatgatcgc
    22141 tggaagcttt agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata
    22201 gcggtaacgc ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat
    22261 cagtagaaat atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt
    22321 aattgcgcgc cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg
    22381 cagggcccgt tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg
    22441 gctggtgctg tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa
    22501 aagcataacg ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta
    22561 atttatcgac attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag
    22621 aaccaataaa ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta
    22681 tagagcatgc tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct
    22741 accgcccctg gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt
    22801 tagccactcg cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag
    22861 aaacaaggaa aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat
    22921 aggctttata aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc
    22981 ttcactgccg ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc
    23041 ttaacagctt ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat
    23101 acttcacaaa cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca
    23161 tcagcattgc atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa
    23221 tacgtcccca tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca
    23281 catagcgacc tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga
    23341 taaattcaac aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga
    23401 cggagtattc agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt
    23461 cgttgaaacg tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt
    23521 cgatcacaac gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac
    23581 acaaaatgga gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc
    23641 ccggcggaca gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga
    23701 gtgattctaa ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag
    23761 ttaccaggag gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca
    23821 ttggcttgtc gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat
    23881 gcatcccgag acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct
    23941 gtaaaatggt tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga
    24001 attgattact gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt
    24061 tcctaagtat ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca
    24121 atctaaagtt aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg
    24181 agctttgccc tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt
    24241 tcgccataaa gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc
    24301 caactgagga ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt
    24361 gtaactccag cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat
    24421 tcccggcgga gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa
    24481 tattattcac tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc
    24541 cgattaacat cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac
    24601 cgtaaatagc gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat
    24661 tgcgggcatt gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc
    24721 gacaatccga tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt
    24781 agttgttatc ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt
    24841 tggtacagtt gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg
    24901 ccacgttcca ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg
    24961 caccgataat ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag
    25021 tgaggttatc gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct
    25081 gcattaccgg gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaaccacgca
    25141 catccagggt aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct
    25201 gtaagacaaa tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc
    25261 cgggagggat gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga
    25321 ccgtttgccc ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt
    25381 cgttaatgtt aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg
    25441 caagcgccga gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt
    25501 ctcctctata aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa
    25561 tcgtgttcga ctttttcgca cgcacgtttg acgaccggag ccagttcgtc ggtatccagt
    25621 tggctaaacg ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag
    25681 ccggatttat cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga
    25741 ataccgaccg ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg
    25801 cccgttaccg atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg
    25861 ctcggtttaa agcccggcat ctctaccaca tcttccagtt gatattgttc gatgatggtg
    25921 cgcaggcgtc gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc
    25981 tctttcaact gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt
    26041 gcgacagata tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc
    26101 gtcatgtcca cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc
    26161 agccttccgg cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt
    26221 tgatattcgg gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag
    26281 atggtggcaa ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc
    26341 gccgggccaa aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa
    26401 atggcagaca aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc
    26461 caggtatttt tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct
    26521 actttgcccg caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc
    26581 catgccgcgt gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag
    26641 cccatatcaa taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat
    26701 ttcaataaga agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca
    26761 ccatctgcat tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca
    26821 ccggaagctg gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac
    26881 gaatatcaat cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt
    26941 catagttgat ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc
    27001 gcgtaccgac ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt
    27061 catccatcac tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca
    27121 ccatgcggtc gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct
    27181 gatagccttc atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt
    27241 gagtggtgcc gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt
    27301 tctgttgtgc ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg
    27361 tgtggtgatc gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat
    27421 ttctgcgttt catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac
    27481 caaagacata gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac
    27541 caatcataaa cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca
    27601 cgccgtagag atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca
    27661 gcaggcgcac gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt
    27721 cggtgacgcg tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc
    27781 gcccgacaac gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca
    27841 ttaccgggcg gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt
    27901 cggtatgtgg attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc
    27961 cacgattgcc gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt
    28021 gatgccaggt gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg
    28081 gcgcgtctta tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt
    28141 acgccgcatc cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag
    28201 cattttcatt ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg
    28261 tgacagcaca atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag
    28321 catccccagc gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt
    28381 cggcaacgag agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc
    28441 aagaaccggt ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag
    28501 gaaaccgagc gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat
    28561 aaacagaaat gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat
    28621 cagcagcgaa ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg
    28681 cagcaccgga ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga
    28741 caccaccatc agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt
    28801 gtagaagtta acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa
    28861 cacgcgggtg atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag
    28921 gttgtatccc cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt
    28981 ggtgttgaga tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg
    29041 tgccacagac gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc
    29101 aaacagcagt gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag
    29161 cggccagaaa tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc
    29221 gatcatgccg attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc
    29281 gtgggggatc accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt
    29341 attcagcacg tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc
    29401 cagcccgacg ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg
    29461 aataatcgag ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag
    29521 cgacacagtc agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg
    29581 caccagcccg aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat
    29641 ggttttttca cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg
    29701 tcaggaaaac gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga
    29761 actcgacgcg tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct
    29821 gcgcccagcc ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct
    29881 ggcgatactg ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc
    29941 cggtcagcac attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc
    30001 ccactttagt gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca
    30061 tcactttcat ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag
    30121 tctggcggaa aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca
    30181 gcaccgggga gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta
    30241 gcaggcggtt aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca
    30301 tctcttcgag acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg
    30361 aacaggtggt atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt
    30421 gcatcgcgat atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca
    30481 ggttacccgc ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca
    30541 cttcaaaccc taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta
    30601 aatcccccgc tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc
    30661 caatgcgaat acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca
    30721 gccagatttt cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa
    30781 tcacgcttaa ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc
    30841 gccatgagcg ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca
    30901 gcgccaccaa caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata
    30961 gcccggcaaa catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat
    31021 tggttttcgc tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct
    31081 tacccgcagt ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc
    31141 gcgttcgcgc ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg
    31201 gttcggtatt ggaggtgcgc aggttaaagc gccagtcggc aaaggtcatg ctgatgccat
    31261 cgctgcgatc caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct
    31321 caacgggttg cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt
    31381 cgcgcaccag ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc
    31441 acgggatcat gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt
    31501 cgccaccgta gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt
    31561 tcgacattac cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac
    31621 gcggatcatg gatgatcttc gcgccgggat ttttttcgag gaacgcttct gccagcaggc
    31681 cgacaatgta gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa
    31741 aatcgccatc aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt
    31801 cgtcgcgaca ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg
    31861 tgttgtgtac tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg
    31921 cgtccaccac cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga
    31981 gatttttgac attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt
    32041 gctgatagcg accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac
    32101 gctggacgtc gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct
    32161 tcatgccgtt gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc
    32221 cgagatggaa cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat
    32281 cgacgcccgc atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac
    32341 ggacatcacc gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa
    32401 tgcgccaggc gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt
    32461 taaagcaggt taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg
    32521 aaacggccaa ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg
    32581 cgacgctgcc gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca
    32641 cgccgcatcc gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga
    32701 agcgcaccac atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg
    32761 gaattttccc cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt
    32821 tttcaccaag cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta
    32881 cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg
    32941 tgatgcgttt cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac
    33001 gatacacttc gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca
    33061 cttttttcac atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca
    33121 ccactaccag atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc
    33181 tgttttcagt tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg
    33241 cgctgatctc ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca
    33301 ccacaacagc atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg
    33361 cgagaaacgc ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg
    33421 ctttttcgca ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc
    33481 cggcgcggaa caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct
    33541 gggcggtttc cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct
    33601 gctcacccac agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg
    33661 gcacaatgcc aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg
    33721 cggcacggaa cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg
    33781 ggtcgctctc cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag
    33841 tgttacgccc tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct
    33901 gttccgcgac aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca
    33961 cgccgttcag gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa
    34021 actgcttggg gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca
    34081 tcacaactgg atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg
    34141 acgtaacacg ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg
    34201 tttgggcagc aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc
    34261 tacgcaaacc gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc
    34321 tgcggtaatc accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac
    34381 ggcatctgcc gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc
    34441 aggtaaagcg tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg
    34501 cgccattttt tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg
    34561 ttcatcgcgc aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat
    34621 attgccggag taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc
    34681 atcaacatcg gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa
    34741 gatgacgtta tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat
    34801 cgtggaaacg ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc
    34861 cactttgccg cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta
    34921 atcctgaata tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg
    34981 cgtgcaaaac agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc
    35041 catcagtgga aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt
    35101 gctcggctgt tttggcacgt acagcgggca gcgccacacc gtggcggccc cttcttctcg
    35161 tttgtagcgc caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc
    35221 ggtaatgacc cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta
    35281 tttgccgatg ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat
    35341 ccgggtactc cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg
    35401 ttactggcga gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc
    35461 agtaacagct cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg
    35521 aaatccgtgc cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct
    35581 gctgttatcg gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc
    35641 agcgtttcgt ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg
    35701 ttggttcttt tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag
    35761 acaagcggtg tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc
    35821 cccgaaagcg gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca
    35881 gtgagatttc gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg
    35941 gcgtaccatc cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg
    36001 cgatggtttg cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg
    36061 acagcatcgg ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc
    36121 tcgccgccgc catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata
    36181 ccaccacgtc cggcgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta
    36241 tcacatggga gttactcggg tggaagttgt catgcggccc gtacaggttg gtcggcatga
    36301 cagtgcggta atcgcgtcca tactggcggt tgtaagattc gcacagtttg atcccggcga
    36361 ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt
    36421 ctgccatcgg ctgttttgcc agtttcgggt agatgcagga cgatccgaga aacatcagtt
    36481 tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt
    36541 agatgaaatc cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca
    36601 gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca
    36661 ggttcagttc atcgcgggta cgtaatacca gctccacatc accgcgctgt tcgagctgcc
    36721 gcttgatggc agaaccgacc atcccacaat ggccagcgat aaaaatgcgt tgtttactca
    36781 tgcttatgac tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt
    36841 tttcgccgct tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc
    36901 cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc
    36961 tgccggacgg aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg
    37021 cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa
    37081 gcgcagtttg atgcccagct gtgctgccgc catttccaca aactgacgta cggagtactg
    37141 aacgccggtc gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat
    37201 ttttacgtag tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag
    37261 gcacgactcc agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa
    37321 ggtttcgccg cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc
    37381 gtaggattcg cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg
    37441 agatcgcggg tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag
    37501 ttcagaggtg gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc
    37561 ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa
    37621 agagaccgca acgtggctca ttgcgcctag gttgtacact tcatccggct gtacttcacg
    37681 caaaatgcgc gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg
    37741 gttgcaggtg tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc
    37801 gcgacgctta ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga
    37861 accgtcttgt ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt
    37921 atttttgaat ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc
    37981 aaaccacatt tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt
    38041 cgccgattgt gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa
    38101 tcacaatagg cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc
    38161 ataaataact tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat
    38221 ataaattgac gtcatcgcca acccacgcgt aatcacctaa ggttaatttc cacggatagg
    38281 taatttttac tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta
    38341 ataaaaaagc ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta
    38401 ctgcccacca taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga
    38461 aaccgcttaa atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttag
    38521 tcttacctgt cgtacgttgg cgtaaatgcc aggataattc tgcccagaag ccaggtacat
    38581 gtaatatctg ccgttggact tttttggcgt cagcac
//
LOCUS       DAJTRM010000028        37631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRM010000028 DAJTRM010000000
VERSION     DAJTRM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.028"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..178)
                     /locus_tag="OO322_001558"
     CDS             complement(<1..178)
                     /locus_tag="OO322_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5906485.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(262..1146)
                     /gene="yhdJ"
                     /locus_tag="OO322_001559"
     CDS             complement(262..1146)
                     /gene="yhdJ"
                     /locus_tag="OO322_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5906486.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1232..1528)
                     /gene="fis"
                     /locus_tag="OO322_001560"
     CDS             complement(1232..1528)
                     /gene="fis"
                     /locus_tag="OO322_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5906487.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1554..2519)
                     /gene="dusB"
                     /locus_tag="OO322_001561"
     CDS             complement(1554..2519)
                     /gene="dusB"
                     /locus_tag="OO322_001561"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5906488.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2848..3729)
                     /gene="prmA"
                     /locus_tag="OO322_001562"
     CDS             complement(2848..3729)
                     /gene="prmA"
                     /locus_tag="OO322_001562"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5906489.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3741..5192)
                     /gene="panF"
                     /locus_tag="OO322_001563"
     CDS             complement(3741..5192)
                     /gene="panF"
                     /locus_tag="OO322_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5906490.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5182..5424)
                     /locus_tag="OO322_001564"
     CDS             complement(5182..5424)
                     /locus_tag="OO322_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5906491.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5533..6882)
                     /gene="accC"
                     /locus_tag="OO322_001565"
     CDS             complement(5533..6882)
                     /gene="accC"
                     /locus_tag="OO322_001565"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5906492.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6893..7363)
                     /gene="accB"
                     /locus_tag="OO322_001566"
                     /gene_synonym="fabE"
     CDS             complement(6893..7363)
                     /gene="accB"
                     /locus_tag="OO322_001566"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5906493.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8341..9315)
                     /locus_tag="OO322_001567"
     CDS             complement(8341..9315)
                     /locus_tag="OO322_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5906494.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9467..11407
                     /gene="csrD"
                     /locus_tag="OO322_001568"
     CDS             9467..11407
                     /gene="csrD"
                     /locus_tag="OO322_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5906495.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11712..12755
                     /gene="mreB"
                     /locus_tag="OO322_001569"
     CDS             11712..12755
                     /gene="mreB"
                     /locus_tag="OO322_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5906496.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12821..13924
                     /gene="mreC"
                     /locus_tag="OO322_001570"
     CDS             12821..13924
                     /gene="mreC"
                     /locus_tag="OO322_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5906497.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13924..14412
                     /gene="mreD"
                     /locus_tag="OO322_001571"
     CDS             13924..14412
                     /gene="mreD"
                     /locus_tag="OO322_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5906498.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14421..15014
                     /gene="yhdE"
                     /locus_tag="OO322_001572"
     CDS             14421..15014
                     /gene="yhdE"
                     /locus_tag="OO322_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5906499.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15004..16473
                     /gene="rng"
                     /locus_tag="OO322_001573"
     CDS             15004..16473
                     /gene="rng"
                     /locus_tag="OO322_001573"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5906500.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16541..20341
                     /gene="yhdP"
                     /locus_tag="OO322_001574"
     CDS             16541..20341
                     /gene="yhdP"
                     /locus_tag="OO322_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5906501.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20497..21942
                     /gene="tldD"
                     /locus_tag="OO322_001575"
     CDS             20497..21942
                     /gene="tldD"
                     /locus_tag="OO322_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5906502.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22070..22999)
                     /gene="aaeR"
                     /locus_tag="OO322_001576"
     CDS             complement(22070..22999)
                     /gene="aaeR"
                     /locus_tag="OO322_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5906503.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23182..23385
                     /locus_tag="OO322_001577"
     CDS             23182..23385
                     /locus_tag="OO322_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5906504.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23393..24325
                     /gene="aaeA"
                     /locus_tag="OO322_001578"
     CDS             23393..24325
                     /gene="aaeA"
                     /locus_tag="OO322_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5906505.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24331..26298
                     /gene="aaeB"
                     /locus_tag="OO322_001579"
     CDS             24331..26298
                     /gene="aaeB"
                     /locus_tag="OO322_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5906506.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26390..26662
                     /locus_tag="OO322_001580"
     CDS             26390..26662
                     /locus_tag="OO322_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5906507.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26718..26981)
                     /gene="yhcN"
                     /locus_tag="OO322_001581"
     CDS             complement(26718..26981)
                     /gene="yhcN"
                     /locus_tag="OO322_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5906508.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27346..27816)
                     /gene="argR"
                     /locus_tag="OO322_001582"
     CDS             complement(27346..27816)
                     /gene="argR"
                     /locus_tag="OO322_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5906509.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28251..29189
                     /gene="mdh"
                     /locus_tag="OO322_001583"
     CDS             28251..29189
                     /gene="mdh"
                     /locus_tag="OO322_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5906510.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29251..30318)
                     /gene="degS"
                     /locus_tag="OO322_001584"
     CDS             complement(29251..30318)
                     /gene="degS"
                     /locus_tag="OO322_001584"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5906511.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30408..31755)
                     /gene="degQ"
                     /locus_tag="OO322_001585"
                     /pseudo
     CDS             complement(30408..31755)
                     /gene="degQ"
                     /locus_tag="OO322_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31909..32307)
                     /locus_tag="OO322_001586"
     CDS             complement(31909..32307)
                     /locus_tag="OO322_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5906512.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32501..33628
                     /gene="zapE"
                     /locus_tag="OO322_001587"
     CDS             32501..33628
                     /gene="zapE"
                     /locus_tag="OO322_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5906513.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33847..34275
                     /gene="rplM"
                     /locus_tag="OO322_001588"
     CDS             33847..34275
                     /gene="rplM"
                     /locus_tag="OO322_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5906514.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34291..34683
                     /gene="rpsI"
                     /locus_tag="OO322_001589"
     CDS             34291..34683
                     /gene="rpsI"
                     /locus_tag="OO322_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5906515.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35078..35716
                     /gene="sspA"
                     /locus_tag="OO322_001590"
     CDS             35078..35716
                     /gene="sspA"
                     /locus_tag="OO322_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5906516.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35722..36219
                     /gene="sspB"
                     /locus_tag="OO322_001591"
     CDS             35722..36219
                     /gene="sspB"
                     /locus_tag="OO322_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5906517.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36262..37629)
                     /gene="dcuC"
                     /locus_tag="OO322_001592"
     CDS             complement(36262..37629)
                     /gene="dcuC"
                     /locus_tag="OO322_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5906518.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9117 a         9415 c         9901 g         9198 t
ORIGIN      
        1 acattctgtc ctgaaaattc agttcgtcac agttaaccag ttttagtgga ttaactgagt
       61 tcatattacg gcatttgtct gtttctgttg tcagcagttc aatccactgc atcagtaaag
      121 tatcaaacag aaaaacgaag acagcgaaaa cgacgagcca ccaatacttg cgaatcatta
      181 cctgagcctg tgcatgttta accaagtctg gcaggaaata tacacgaaat gaataatctg
      241 aaaactaggc ttacacttcc tttactttgc aatgagatcg gggtcaactt cgcttaaccg
      301 gcttcgtttt gacaggttgc cggtctttct ttttttcact ttcgccagtt cttccgcgga
      361 gtaatgcgac gcgacatcca gccgtcgaag ccccattttg atgtactcgc tgttgatctc
      421 aataccaatg aattttcgtc cgctggcgat ggctacggca ccggtagtaa agctaccggc
      481 aaacgggtcg agaacgatat cgcctgggtt ggaagaggcg agaataatgc gtttcagtaa
      541 ggctttcggt ttttgcgtcg ggtggttctc atattcatcc atcaaataac gcacgcgcgg
      601 aaaatcccag acgttacccg gtactttttg atgattgtat ggctgtggag gatttttgcg
      661 ataatcgatc aacgcgcgct gcgatccggt tttggcttct accagaatag catcaccgtt
      721 gaatgtgtag ttctttgcgt ctttcaccat catcaggatg ggttcgtaca tggagccgta
      781 gtgttttttc gcctgtactc cagaactgtc atatgaccag acgatgcgac ttttgatggt
      841 aaaaagcttg cggcactgga gatcgataaa gggcatgttt tccgtactgt tcataatgta
      901 catgctgccc tgctttttca gaacgcggtg gcactctgca atcacttcaa acagccagtc
      961 gataaacaga tcttctttcc aggcttcgat cagaccatca aaatttttac cgatgttata
     1021 cggtgggtcg gcaaagagca gatcgacgct ttcagtaggt agctttttaa gttcggcaaa
     1081 ggcatcaccg tgaataatgg tcttagcttc attaccaaac cgggtcggtt cacatcctgt
     1141 tctcatggtc actccctttg tgacacctat aaaaaaggcg cttccccatg ccgagtagcg
     1201 cctttttaat caagcattta gctaacctga attagttcat gccgtatttt ttcaattttt
     1261 tacgcagcgt accacggttg atgcccatca tcagcgcagc acgggtctgg ttaccacggg
     1321 tgtattgcat caccatgtcc aacaggggct gttctacttc agccagtacc agctcataga
     1381 ggtcattcac atcctgacca ttcagttgag caaaatagtt cttcagtgcc tgtttaaccg
     1441 agtcacgcag gggtttttgg gttacctgat cctgagagtt aacggtagaa acggtcagta
     1501 cgtcagaatt tacgcgttgt tcgaacatag ttctgtcagc tctttatttc tgtttacgca
     1561 aaattttcga agtatgcctc caacgcctcc agctgttcgc tggcatcctc aatggcgttg
     1621 aatgtgcgcc gaaactggtc atttggagcg tgttcctgga gataccagga aacgtgttta
     1681 cgtgcaattc ggtacccttt tgccggacca taaaagtcat gcagttcccg aacgtgcgcg
     1741 caaagcaagc gcttaacctc tgccaaaggc agtgggggca gcaactcccc agtgtccaga
     1801 taatgctgga tttcccgaaa gatccagggt cttccctgag ctgcgcggcc tatcatcagg
     1861 gcatccgccc ctgtatagtc gagcacagct ctggctttaa gcgggtcagt aatgtcgcca
     1921 ttcgcgataa ccggaatgga aactttctgc ttaactgccc gaatactgtc gtactcagct
     1981 tctccattga acaaacaggc gcgtgtacgg ccatgaatgg tcagagcctg aatgccacag
     2041 tcttcagcca gttgggcaat ctcttcgcag ttacggtgtt ccggtgccca gccggtgcga
     2101 atcttcaggg taacaggaac gtccactgca ttgacgacct cggtaaggat cgatttaacg
     2161 acatccgggt actgcaagag ggctgaacct gcgagcttgc gattcacttt tttagccggg
     2221 caacccatat tgatatcaat aatctgggca ccgctttcca cgttaatacg tgctgcatct
     2281 gccatttctt tcggagcgct accagcaatt tgcacggtgc gaataccggg ttcatcaatg
     2341 tgcaccatcc gtaaacgaga tttgtcgctt tcccaaacct gtgggttaga agacatcatc
     2401 tcggatactg tcaatccggc tcccatctcg tagcacaacg tccgaaaagg tctgtctgta
     2461 atgccagcca tgggcgctgc gatcaggcga tttctgagct gatattgtcc gatgcgcatg
     2521 agttaagaaa tgaccatact gtgactgcaa ggcggcgtat attacgcatt ttttgcacga
     2581 gatgaaaggc caaactttga ccaatcctct gagatggatc aaagaattgc atttaaaatg
     2641 agcgtggtgc gataattact cataaaaatc atcatattag aaaatagtga ctaaaattta
     2701 cactcaaaga aatttgagta agttctcaat ttttctttat gaatgaaaat tttggcacgc
     2761 aaatttgcgt aaataatcgg caaatttacg tgccttttgt gagcttgctc gcacttcgcc
     2821 ccgcgtcacc ctacggcgat gcgaaggtca attcttacga ccggtaatac ggcaccactc
     2881 ttctttttcc acgaccgggt ccagtgcgaa gctatcggca taagcttcac aaacgctctc
     2941 tgcctggctt gccagaatac cggaaaggcc cagcaaaccg cctgaaactg gcaggacgct
     3001 gattaacggt gccagttcac gtaatgggcc tgcaaggatg ttagcgacca ccacgtcggc
     3061 tttcatttct tctggctgat ctttcggtaa gtagagttcc agacggtcag aaacgccatt
     3121 acgttcggcg ttatcgcggc tggcctgaat cgcctgcgga tcgatatcaa taccaatggc
     3181 ttttgctgca cccagtttca gcgccgcgat cgccagaatg ccggaaccac agccaaagtc
     3241 gatgactgtt ttaccggtta aatcgaggct gtcgagccat tgcaggcaca gagaggtggt
     3301 tggatgggta cccgtaccaa acgccagccc tggatctaac atcacgttga cggcgttttc
     3361 gtccggcaca tcacgccagc tagggcagat ccacagtcgt tcaccaaagc gcatcgggtg
     3421 gaaattatcc atccattcgc gctcccagtc tttatcttct agttgttcga ttttatgcgc
     3481 gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc accacgtcgt tcatatcggt
     3541 ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac aggcgcgttt cgcccggcag
     3601 cggctcaaat actggcgtat cgtgggtatc ctgaaaagtg atagaaacgg cacccgcttc
     3661 catcagcgca tcgctaagat cttccgcgtt cgcgccggtg gtgttcagtt tcagttggat
     3721 ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa cggtagcttg cgggacggat
     3781 gtaccgaaac ggtttccgac caggaaagcc agcaaactta gtagtaacga gggcacgata
     3841 gggtggaagc ccaggtactg aatattcagc gtcgcgagta cggcatacag cacgccgcca
     3901 acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt cccagtaaag acccagcacc
     3961 agcggccaca ggaaaacggc ttccagccca ccgaaggcca gcaaattcag ccagatgatc
     4021 atttctggcg gcttccaggc ggcaagcagc agcaacgcgc cgagaactaa cgtaattacc
     4081 gccgacatcc gcttcagacg cgtctcgttt tgcatttgat ccggacggat attcagatag
     4141 agatctttaa tgatcgtagc ggaactttgc agcagttggg cgttaattgt cgacatgatc
     4201 gcagccatag gtgcagccag aaagatcccg gcagcaaaag gtggcagcac ttttaccatt
     4261 aacgtcggga tcaccagatc tggtacggtg agatcgggga tcactgcacg acctaacgct
     4321 ccggccaggt gcataccgaa catcaggatt gcgactataa tcgtaccgat gatgatcccc
     4381 cggtgtactg ctttgctgtc tttataagag atacagcgca ccgcagtatg cggcaggcca
     4441 atcacgccaa agcacaccag tacccagaac gacgtcataa aggcaggcga cagaatatcg
     4501 tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt gcaaggtctg tactgcgttg
     4561 cttaagccgc cagcggcatg taccacgcca ataagcagca caacggtgcc aatcagcatc
     4621 acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa agccaccaaa ggcggtatat
     4681 aacgcaatgc tgataccaaa aatcagcagc ccggtttcat aaggaatacc cgccgcggtt
     4741 tccagcaggc gcgcaccgcc gataaactgc acggtcatcg caccaacgaa cgcaaccagc
     4801 aaactcaaac tcgccagcca caccagaaga cgactctggt agcgggcaaa cagcatatcg
     4861 ttcagcgtca ctgcattgta gcggcgcgca agaatcgcaa acttcttgcc gagaataccg
     4921 agtgaaagcc agacagcagg aagctgaatc atcgccagca atacccagcc cagcccgtat
     4981 ttataagcag ctcctggccc gccgataaac gaactggcac tgatataggt cgcggtgaga
     5041 gtcatcgcca gcacaatacc gcccatagag cggctgccga ggaaatactc attaaggaag
     5101 gtgccggtgc tccgtttacg catcgcataa accgagatac cgaacaccac caccagatag
     5161 gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc tccagtggga tatcgcgata
     5221 gataaatttc accatcgccc agcacagtcc aataaacagt agcggcgtca ggatgcaggc
     5281 catctcaaac cagcgcggaa agccggtaaa accgggggca acgccaggta agtaagcgtc
     5341 tactaaccaa actgccagat acaaaagggt cagccccagc gcccagcggg cctctttatg
     5401 ggcctgaaca aaacgagtgt ccattttttg tccctgatgg gtgaagaaag cgaggattgt
     5461 accttatggg ggttgtcgat ccccagtaat aaaaaaggcc ggaaaatccg gccttttgac
     5521 gcttttgcag tcttattttt cctgaagacc gagttttttc tccagatagt ggatgttagt
     5581 gccaccatgc tggaagttct cgtcattcat gatgcggatc tgcagatcaa cgttggtttt
     5641 gataccgtcg atgatcagct cctgcagcgc attcttcatg cgggcaatcg ccacgtcacg
     5701 gttttcaccg tagcaaatca gcttaccgat cattgagtca tagtacggcg gtacggtgta
     5761 gcccgcgtag atatgagact cccaacgtac gccaaaaccg ccaggtgcgt ggaaacgggt
     5821 gattttgcct gggcttggca ggaaggtgtt cggatcttcg gcgttgatac gacattccac
     5881 cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac agcggttgac cggcagcgat
     5941 acgcagctgt tctttgatca ggtcaacgcc ggtgatcatt tctgtaaccg ggtgttctac
     6001 ctgaatacgg gtgttcattt cgatgaaata gaactcgccg ttttcgaaca ggaactcgaa
     6061 agtacctgca ccgcgatagc cgatatcaac acacgcttta gcgcaacgtt cgccgatgta
     6121 gcgacgcagt tccggggtaa tgcccggtgc tggtgcttct tcgaccactt tctggtggcg
     6181 gcgctgcatg gagcagtcac gttccgccag atagatagcg ttgccctgac cgtcagccag
     6241 tacctgaatc tcgacgtggc gaggattctc caggtatttc tccatgtaaa ccatatcgtt
     6301 gctgaaagca gctttcgctt ccgcacgggt catggagatg gattgtgcca gttcagcgtc
     6361 gccgcgcact acgcgcatac cgcgaccgcc gccgccgccg gaggctttga taatcaccgg
     6421 ataaccaatg cgtttagcaa tggcgcggtt tttatccata tcgtcgccca gcgggccgtc
     6481 agaaccaggt acgcaaggaa cgcccgcttt tttcatcgcc gcgattgcgg atactttgtc
     6541 gcccatcagg cgaatggttt ctgctttcgg gccgatgaag ataaagccgg agcgttcaac
     6601 ctgctcggca aagttggcgt tctcggagag gaagccgtaa cccggatgga ttgctactgc
     6661 gccggtgatt tcagcggcgc tgatgattgc cgggatgttc agataacttt ttactgacgg
     6721 agcagggcca atacagaccg tttcatctgc cagtaatacg tgttttagat cgcgatccgt
     6781 gctggagtgc acagcgacag tcttgatgcc cagttcttta caggcacgaa gaatacgcaa
     6841 tgcaatctcg ccgcggttgg caataacaat tttatccagc atgttcgcct cgttactcga
     6901 tgacgaccag cggctcgtca aattctaccg gttgtccact ttcgaccaga attgctttca
     6961 cggtaccgga tttgtccgct tcgatctggt tcatcatttt catggcttca acgatgcaca
     7021 gggtatcgcc cacgttgact ttctgaccca cttcgatgaa cgcttttgcg tccgggcttg
     7081 gggtgcggta gaaagtacca accatcgggg aacgtacgat gtgaccactg atttccgctg
     7141 ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc tgcgttagat tgagctggct
     7201 gctgcatcat tggtgcagcg taagcttgtt gcatcacagg gaaacttgcg gcaggagctg
     7261 cacggctaat gcgtactgac tcttcgcctt cagaaatttc cagttcggag atgcctgatt
     7321 cttcaaccag ctcgatcagt tttttaatct tacgaatatc catgagtggg ttccgtactc
     7381 tttgtttagt gtgattgtga caggcgtttc accgccgtct gtaaagcgta tgccccgaca
     7441 gctgtatgca tagcgataaa ttccagcagg ccgggacgcc ggtctatttt gccccagagt
     7501 gtcaatgtta gacttgacgg acattgtgca gcgatctgct gctatcctcc ggcaaaaaac
     7561 aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag cgcaaaaact gcgtcaggga
     7621 ggacgaggtc gcacattata acgatttcgt agcaattggc agctaaatac tggtcttatc
     7681 agggaatata atcaacagct aacatgtaaa taaccttcaa caccgtgtaa tttgcaacaa
     7741 gccgcacaat tcacgaaatt agcgccatca tcgacggaac ttcttataac gtaaggtgaa
     7801 aagctgaaaa gccagccctg cgaagatgag gggctgcaat gagataatct tcacagacca
     7861 caaataatgt agggatgcca ggatcgcgac aagatagacg acattacgca gcggttgctg
     7921 gggattaccc aattttcact gcatcgtctg gatagatgtg aatgctaaag cacgcaaaat
     7981 caaccaactg acaattctca gcattaaata ggttcgagtg gttaatttgt agtcaaataa
     8041 aatcagatta ttcaccccat ggtaccagaa atcagtaaca ggttaagcgt agtgtcgccc
     8101 aggcaaagca ccataaacct aacaggcgac ggaggcgtat caataatggc tgtttagagg
     8161 agtatcaatg cgcatcaatt tgtggtcagt cgaaatcatg actttccagt ttggatgatg
     8221 ttgtacaact tcctgaccaa ggtgttcgac gaaagctgtc cggctaaccg cttttcccgt
     8281 taaccgattg aataatgatg tcaaaatgag ttccctctct tattattcct tgctaaatgg
     8341 ttagttaacc ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg cctcggcaaa
     8401 gttcggtgcc tctgacagag atatctcttt tgccgcctgg gtatagaatg attccggtaa
     8461 atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct ggtggcgtca ttactgaatc
     8521 caccccttgc aaacggacat tacgcagaat aaatggcatg accgtggttg gcagagtaaa
     8581 accacccgcc agaccacagg ccgccacgca gccgccgtaa ttcatttgcg ccagcacttt
     8641 tgccagcact ttgtcgccaa cagtgtcaat tgccccagcc cagacttgtt tttccagagg
     8701 acgggattcg gcaaactcat cacgagggag aataccgctg gcacctaaac ttttcagata
     8761 ttcatgggta ctttcgcgac cggaaacggc aacgacctga taacccaact tatgcagcag
     8821 cgccacggcg gtactgccga cgccaccact ggcacccgtc acgacaatct caccgtcctg
     8881 cgggcgaaca ccggcatctt ccagcgccat cacacacagc atggcggtaa aaccggcagt
     8941 accgataatc attgctttac gcgcgtccag cccttgcggc atggcaacca gccagtcacc
     9001 tttcactcgc gcctgctccg ccagcccacc ccagtggttt tcaccaacgc cccagccagt
     9061 gagtaacacc tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta cagttccggc
     9121 aaaatcgatc ccaggaatca tcggaaaatt acggatgatt tttcccttac cggtaatcgc
     9181 cagcgcatct ttatagttca ggctcgacca gtgaacatcg accgtgacat cgccctccgg
     9241 caggcgacgt tcgtccagag tctgtactga tgcgagagtt ttgccgtcct gctgttctaa
     9301 aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga ttggaaaata atttctgaag
     9361 actatactcg ctaatggaaa agcaaagccg atgaagcgca ataaattgcg agataaatct
     9421 gatttgctag tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac
     9481 gaaattttcg gcctttgtta cgctgctcac cgggttaaca atttttgtga ctttgctggg
     9541 ctgttcgcta agtttctaca acgccattca gtataagttt agtcatcgcg ttcaggcggt
     9601 ggcgacggcg attgataccc accttgtgtc gaatgacttc agcgcattaa ggccacaaat
     9661 taccgaatta atggtgtcag cagatatcgt tcgtgtagac ctgctgcatg gtgataaaca
     9721 ggtttatacc ctggccagaa atggtagtta tcgtccggtt ggcaccagcg atctgtttcg
     9781 tgaactgagc gttccgttga taaagcatcc ggggatgtcg ttgcgtctgg tttatcagga
     9841 tccgatgggc aactatttcc attcgttgat gaccaccgcg ccgctcacgg gggcgattgg
     9901 ctttatcatt cttatgctct ttctggcggt acgctggtta caacggcaac ttgccgggca
     9961 agaattgctg gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt
    10021 gggaaccatc tatgaatggc cgcccagaac cagcagtgcg ctggatacgc tgcttcgtga
    10081 aattcagaac gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc
    10141 acaggacgtg aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt
    10201 actggaagat caggagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga
    10261 tttcaatatg ttgagcgata cctgggggca cagccaggtt gaagaacagt tcttcactct
    10321 gacgaatctg ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca
    10381 ccgcagtgat tttgctgcgc tgttaccgca ccggacgtta aaagaggcag agagcatagc
    10441 cagtcagtta atcaaagccg tcgatacctt gccgaacaat aaaatgctcg atcgcgacga
    10501 tatgatccac attggtatct gtgcctggcg tagtggtcag gataccgagc aggtaatgga
    10561 acatgcagag tctgccacgc gtaatgcggg attgcagggt ggcaatagct gggctattta
    10621 tgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca
    10681 aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggtca
    10741 ggttcatcat cgcgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc
    10801 ggagtatatg ccgatggtct tgcagtttgg cttatcggaa gagtatgacc gtctgcaaat
    10861 cagccgtctt attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac
    10921 cgttgagtcg ctgattcgtc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg
    10981 tgaaaaatca caacgaaaac gcataattat tgaacttgca gaggccgatg taggtcaaca
    11041 tatcagtcgt ttacaacctg ttattcgttt agtgaatgct ttaggggtac gggtagccgt
    11101 caaccaggct ggtttgacgc tggtaagcac cagttggatc aaagaactta atgttgagtt
    11161 actcaagctc catccggggc tggtcaggaa cattgagaag cgaacggaga accagctgct
    11221 ggttcaaagc ctggtggaag cctgctccgg gaccagcacc cacgtttacg ccaccggcgt
    11281 gcgttcgcga agcgagtggc agaccctgat tcagcgcggt gttacaggcg ggcaagggga
    11341 ttttttcgcg tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc
    11401 ggtttaacct gccgtttaat ccgttttcac gtagaataac gcgcgctgcg tctcatggga
    11461 gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg
    11521 ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt
    11581 tgtaacaaag gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt
    11641 cgctgctgcg tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat
    11701 tatcccttag tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc
    11761 tgggtactgc gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt
    11821 ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc
    11881 atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga
    11941 aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac
    12001 aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtaccggttg
    12061 gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg
    12121 aagtcttcct gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg
    12181 aagcgaccgg ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct
    12241 ccttgaacgg cgtggtttac tcctcttccg tgcgcattgg tggtgatcgt ttcgacgaag
    12301 ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc accgcagaac
    12361 gtatcaagca cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa atcgaagttc
    12421 gtggccgtaa cctggcagaa ggtgttccac gcggttttac cctgaactcc aacgaaatcc
    12481 tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac
    12541 agtgcccgcc ggaactggct tccgacatct ccgagcgcgg catggtgctc accggtggtg
    12601 gcgcactgct gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg
    12661 ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg
    12721 acatgcacgg cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaagcgtct
    12781 gtttaccctg cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg
    12841 tggcccgtcg ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat
    12901 tgccgacagc cgcctgggga cgttcagtca aatccgtact tatatggata ccgccgtcag
    12961 tcctttctac tttgtttcca atgctcctcg tgaattgctg gatggcatat cgcagacgct
    13021 ggcctcgcgt gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa
    13081 aaacagtgaa ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct
    13141 gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt
    13201 taacgatcct tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga
    13261 aggccagccg gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact
    13321 gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg
    13381 caacgatatc cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca
    13441 tctgccagcg aatacggata ttcgtgttgg tgatgtgctg gtgacctccg gtctgggcgg
    13501 tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg
    13561 cgcttatact gtgatccagg cgcgtccgac tgcaggactg caacgtttgc gttatctgct
    13621 gctgctgtgg ggcgcggatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg
    13681 tgttgctaat gaacgtctga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat
    13741 ggggccaaag ttacctgaac cggcaacggg gatcgctcag ccgactccgc agcaaccggc
    13801 gacaggaaat gcagctactg cgcctgttgc gccgacacag cccgctgcta atcgctctcc
    13861 acaaagggct acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggagggca
    13921 atagtggcga gctatcgtag ccagggacgc tgggtaatct ggctctcttt cctcattgcg
    13981 ctgttgctgc aaatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg
    14041 ttacttatct tgttgtattg gatcctggcc ttgccccatc gcgtaaatgt gggcacaggt
    14101 tttgtgatgg gtgccatact ggatctgatc agcggctcga tgcttggcgt acgcgtattg
    14161 gcgatgagca tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca
    14221 ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc
    14281 tgggcagagt ttttagtgat taacgtctct ttcagaccgg aagtgttctg gagtagtgta
    14341 gtcaatgggg tgctctggcc gtggattttc ttgctgatgc gtaaagtccg tcagcagttt
    14401 gcagtgcaat aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc
    14461 aggagttact tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc attgaggagc
    14521 agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg tctggcacgc gagaaagcac
    14581 aggcgggtgt cgcgcaaacg gcacaggatc tcccggtgct gggtgcggat attatcgtta
    14641 tcctgaacgg agaagtgctc gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc
    14701 gcaaattatc gggtcagacc catcaggtga tgacggcagt ggcgttggcc gacagccagc
    14761 acattctcga ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca
    14821 tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac ggtattcagg
    14881 ggctgggtgg ctgttttgtc aggaagataa atggcagcta tcacgccgta gtcggcttac
    14941 cgctggttga aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata
    15001 aacatgacgg ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt ggcgtatatt
    15061 gatggcggta ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc
    15121 aatatctaca agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat
    15181 attgggctgg ataaagccgc gtttcttcat gcatccgaca tcatgccgca taccgaatgt
    15241 gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggttcgtcag
    15301 gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg
    15361 accaccgata tcacgctgcc ttctcgctat ctggtattta tgccaggggc ttctcacgtt
    15421 ggggtttccc aacgaattga aagcgaatca gaacgtgaac gtctgaaaaa agtggtcgca
    15481 gagtattgcg acgagcaggg cgggtttatc atccgcaccg cagcggaagg ggttggcgag
    15541 gctgaactgg cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt
    15601 aaaaagcgcc cgcagacccg ttatcagctg tacggcgaac tggcgctggc gcagcgtgtt
    15661 ctgcgtgatt ttgccgacgc cgaactggac cgcattcgcg ttgactcacg cctgacttac
    15721 gaagcattgc tggagtttac ctcggagtac attcccgaga tgaccagcaa gctggagcat
    15781 tacaccggac gccagccgat tttcgatctc tttgatgtcg aaaacgaaat ccagcgagcg
    15841 ttggaacgca aagtagaact gaaatccggt ggttatctga ttatcgacca gaccgaagcg
    15901 atgaccaccg tggacatcaa taccggagcg tttgtcggtc atcgcaatct ggacgacact
    15961 attttcaata ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgtaat
    16021 ctgggcggga ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgccgc
    16081 gtgctgcatt cgctggagca ggcgttgagc aaagaccggg tgaaaaccag cgttaatggt
    16141 ttttcggcgc tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac
    16201 gtactgtgta acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg
    16261 gtatgctatg aaatcatgcg cgagattgtt cgtgtccacc atgcttacga ctccgaccgt
    16321 ttcctggtct atgcttctcc ggcagtagcc gaagccttga aaggcgaaga gtcacactcg
    16381 ctggcggaag tggaaatttt cgttggcaaa caggttaaag tacaaattga accgctctat
    16441 aaccaggagc agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa
    16501 gggtttttga gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat
    16561 tttactgctt actggagccg cgctcgttgt gatcgctgcc ctgctggtta gcggcctgcg
    16621 tattgcttta ccgcatctta acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc
    16681 gactggcatg ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggcccgac
    16741 gcttgaagca cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg
    16801 cgttactctg gcgctggatg tctggcagag cctgttacat atgcgctggc agtttcgcga
    16861 cctcactttc tggcagctgc gctttcgcac caacactcct atcaccagcg gtggtagcga
    16921 tgacagtctg gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga
    16981 tctccgcgac agtgaagtca gttttctgac gccatccggt cagcgcgccg agctggcgat
    17041 cccacaactc acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct
    17101 ctccagcctt accggacagc acggcgtgat gcaggtgcgc atggatttgc gcgatgatga
    17161 ggggttgtta agcaatggtc gcgtctggct acaggcggat gacatcgacc tgaagccgtg
    17221 gctcggtaaa tggatgcagg acaatattgc tctggaaacg gcacagttct cccttgaagg
    17281 ctggatgacg atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg
    17341 tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg gtggataatc tgaccgcgca
    17401 tattacgcgt gaaaatccgg gctggcagtt ctctattccc gatacacgga tcacgatgga
    17461 cggcaaaccc tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg
    17521 cggcaaagac aataaacgca gtgacgaact ccggattcgc gctagtaatc tggagctggc
    17581 aggcctggag ggcatacgcc cgctggccgc gaaactttca cctgcactgg gtgatgtttg
    17641 gcgctccaca caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc
    17701 ggcagacaag acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt
    17761 attaccgggt gcggaacact tctccgggac gctttccggc agcgttgaaa atggtttgct
    17821 taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactgga
    17881 aatcgccgac ggccaggcaa ctataagctg gctgaacaat gacaaaggtt tccagctgga
    17941 tgggcgtaat attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct
    18001 gcaacctgct aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg
    18061 ttcacaagcc tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattattt
    18121 aagtggtgcg attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc
    18181 gcaactcttc ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa
    18241 cgcgaagttt gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga
    18301 ctttattaac gacggtttat ggatgaaaac cgatggcgtt aatctgggcg gcgtgcgcgc
    18361 gagtaatctc accgcagtga tccctgacta ctcaaaagaa aaactgctga ttgacgctga
    18421 cattaaaggt ccgggtaaag ccgttggccc ttactttgat gagacaccgc tgaaagattc
    18481 tctgggtgcg accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct
    18541 tgatatcccg ctgaacggcg aactggtaac cgcgaaaggt gaagtgacgc tgcgtaataa
    18601 cagtctgttt atcaaaccac tcgacagcat cctgaaaaat ttgagcggta aattcagctt
    18661 tatcaatggc gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa
    18721 cgtggatttt tccaccaaag aaggggcaaa agcctaccag gttgcggtga atctcaacgg
    18781 taactggcaa ccggcgaaaa ccggcgttct gcctgaagcg gtgaacgaag cattgagtgg
    18841 cagcgtggcg tgggatggta aagtgggcat tgatctgcct tatcatgctg gcgcgaccta
    18901 taacgtagag ctgaacggcg atctgaataa tgtgagtagt cacttacctt caccgttagc
    18961 caaacctgcg ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt
    19021 tgaattaacc ggacaggctg gtgcggataa ccatttcaat agccgctggt tgcttggtca
    19081 aaagctgacg ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc
    19141 ggaacaaagt ggcgttgaac ttaatatgcc gccgatgaat ggtgccgagt ggctggccct
    19201 gttccagaaa ggcgctgcgg agagtgtcgg tggtgcagcg agtttcccac aacacataac
    19261 gttacgtacg cctatgttgt cactgggaaa tcagcaatgg aataacctga gtattgtttc
    19321 gcaaccgacg gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca acgccacgct
    19381 agcgatgcgt aataacgcgc cgtggctggc gaatatcaaa tatctttatt acaacccgag
    19441 cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct ttcccgacaa cggagcgcat
    19501 taacttccgt ggctggccgg acgcacaaat acgatgcaca gagtgctggt tctgggggca
    19561 aaaattcggt cgcattgaca gtgatatcac catttctggc gatacgttaa cgctgaccaa
    19621 tggactgatt gatactggtt tctcgcggct tactgccgat ggtgaatggg ttaataatcc
    19681 ggggaatgaa cgtacctcgc tgaaaggaaa actgcgtggg cagaaaattg atgccgccgc
    19741 agaatttttt ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt
    19801 acactggcgc aaagcaccct ggcagccaga tgaagcgacg ttgaatggca tcattcatac
    19861 tcaactgggt aaaggcgaaa ttaccgaaat caataccgga catgccgggc aattgctgcg
    19921 cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg
    19981 cgaagggttc tattttgact ccattcgcag caccgcgtgg attaaagacg gcgttatgca
    20041 caccgacgac acgctggtgg atggcctgga ggcggatatc gccatgaaag ggtcggtaaa
    20101 tctggtacgc cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt
    20161 gggcgtggct gcggcttttg cggttaaccc cattgttggc gcggcagtgt ttgccgccag
    20221 taaagtgctg gggccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc
    20281 gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa aagcgcaatg
    20341 atttgacgag ggcgcgtaat tgccccaatc tcatatgata atcgttgcca aaggccaacg
    20401 agccggaaca taactgtagg ccggataggg cgttcacgcc gcatccggca gccgtaaaaa
    20461 atcctctact gcagtaacta acgagtagca aaaacgatga gtctgaacct ggtaagtgaa
    20521 caattgctag cggcgaacgg cctgaaacat caggacttgt tcgcgatcct cggtcaactg
    20581 gccgaacgtc gccttgatta tggcgatctc tattttcagt cgagctatca cgaatcctgg
    20641 gttttagaag accgcattat taaagatggt tcttacaaca tcgatcaggg cgttggcgtg
    20701 cgtgcaatca gcggtgaaaa aaccggattt gcttacgctg accaaatcag cctgctggcg
    20761 ctggaacaga gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga tggcaaagta
    20821 cagacgctgg gcgcggtaga gcatagcccg ttgtatacct cggtagatcc gctgcaaagc
    20881 atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc tcgcgaagcg
    20941 gacaagcgcg tacaggaagt gactgccagc ctcagtggtg tctatgaatt aattctggtt
    21001 gcggccaccg acggcacgct agcggcggat gtccgtccgc tggtgcgtct ttccgtgagc
    21061 gttctggtcg aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg cggtcgtttt
    21121 ggttatgagt tcttccttgc cgatcttgac ggcgaagtcc gtgcggatgc atgggcaaaa
    21181 gaagcagtgc gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc gggcaccatg
    21241 ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt aggtcacggt
    21301 ctggaaggcg acttcaaccg ccgtggcact tcagtattta gtggacaggt cggggagctg
    21361 gtggcttcag aactgtgtac cgtggttgat gacggcacga tggtcgatcg ccgtggttcg
    21421 gtggcgattg atgatgaagg tacgccaggc cagtacaacg tgctgattga gaacggcatt
    21481 ctgaaaggct acatgcagga taaactcaac gcgcgtttga tggggatgac gccgactggc
    21541 aacggtcgcc gtgaatccta cgcccatctg cccatgccgc gtatgaccaa cacctatatg
    21601 ctgccgggta aatcgacccc gcaagaaatt attgaatccg ttgagtacgg tatctatgca
    21661 ccgaactttg gtggcggtca ggtggatatc acctccggca aattcgtttt ctccacttca
    21721 gaagcatatc tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc aacgttgatt
    21781 ggttccggta tcgaaaccat gcagcagatt tcgatggttg gcaacgacct gaaactggat
    21841 aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt gggccagcca
    21901 acgttgaaag tcgataacct gactgttggc ggtactgcgt aataatttca ttattttcag
    21961 aaggataatt aatctttcta cgtgcacgaa cggtcccctc gctcctctgg ggttagggtg
    22021 aggggaaccc gttggcacag gtttgtagaa cgtaacagta caatatgaat tacttctctt
    22081 tcccgcgccc gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg
    22141 agttgatcac gacctgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa
    22201 ccgggcgtgg atctgactga taacgcggca gcaggatctc cagctcccca cgattgatct
    22261 cgttgatcac ccacatcagc ggcacgtagg cgatcccggc acccgccgtt agccagcgca
    22321 ccagcgtcat cggatcatta gtcacaaatc tcccttgcgg gatcaggcga gtcgagatcc
    22381 cttccggtgc aatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat
    22441 ggctacttaa atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg
    22501 cgcacaccac cattggcatc gcgccaagac ggcgggaaaa caggctggaa tcctgcaacg
    22561 cgccgacgcg gatcaccaca tccagaccgt cggcaatcag gtcgggggct ggaattccgg
    22621 taaccagatt ggcgctcaaa cctgggtatt ctttcagcat tttggctgtc agcccggcga
    22681 gaacattttg tgccatagtt gaagaacagc caatgcgtag cgtcccgatg ggggtgttat
    22741 tgaaggcata cagttgctca tgaacatcct gcacttcatg aagcatacga cggcagccct
    22801 ggtagtaaat tctaccggct tcggtcaggc caatgctgcg tgtgctacgg tttaacagct
    22861 ttacctgcaa ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct
    22921 gtagctgtct ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca
    22981 tgcgttttag tcgttccatt attcactctg acttaaaagt gatttagatc acataatata
    23041 gataacagca taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc
    23101 gcccggcttt ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct
    23161 ctctatacaa ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc
    23221 ttcccaccga tattttttga attgctttta tcactggcga ttttctggct ggtgcgccgg
    23281 gtacttgtgc caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg
    23341 ctctattgct gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac
    23401 actaataaga aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat
    23461 cgcaattttt aatgcctggg tctattacac cgaatccccc tggacgcgtg acgcgcgctt
    23521 tagcgctgac gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt
    23581 tcatgataac cagctggtga aaaaaggaca gatactgttc accatcgacc agccgcgcta
    23641 tcaaaaggcg cttgaggaag cgcaagccga tgttgcttat tatcaggtac tggcacagga
    23701 gaaacgccag gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt ctcgcgaaga
    23761 gatcgaccag gctaacaacg tactacaaac ggttctgcat cagttagcga aagcgcaggc
    23821 gacccgcgat ctggcaaaac tggatcttga acgcacggtg atccgagcgc cagcagatgg
    23881 ctgggtgacc aacctcaacg tctataccgg tgagtttatt actcgaggat caacggcggt
    23941 tgcgctggtg aaacagaact ccttctatgt actggcctat atggaagaaa ctaagctgga
    24001 aggggtgcgt ccggggtatc gtgcagagat cacgccgctt ggcagtaaca aagtgctgaa
    24061 agggactgtt gatagtgttg ccgctggggt caccaacgcc agcagcacgc gtgacgacaa
    24121 agggatggcg accatcgact ctgaccttga atgggtgcgt ttggcgcaac gtgttccggt
    24181 tcgtattcgt ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacagt
    24241 ggtggtcact ggcaaacaag atcgcgacga aagccaggat tcgttcttcc gtaaaatggc
    24301 ccatcgcctg cgtgagtttg gttaatcacg atgggtattt tctccattgc taaccaacat
    24361 attcgctttg cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac
    24421 ttccagctgg aaacgccacg ctgggcggta ctgacagcgg cgattgttgc cgccggtccg
    24481 gcctttgctg cgggaggtga accgtattct ggcgctattc gctatcgtgg ctttttgcgc
    24541 atcatcggca catttattgg ctgtattgcc ggactggtga tcatcattgc gatgatccgc
    24601 gcaccattat tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc
    24661 tcgctggtac gaatagaaaa ctcgtacgcg tgggggctgg ccggttatac cgcgctgatc
    24721 attgtgatca ccattcagcc ggaaccattg cttacgccgc agtttgccgt cgaacgttgt
    24781 agcgagatcg ttatcggtat tgtgtgtgcg attatggcgg atttgctctt ttctccgcga
    24841 tcgatcaaac aagaagtaga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta
    24901 atgcaactct gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgacctg
    24961 gtgcgacgca ccacggcact acaaggcatg cgcagcaacc tgaatatgga atcttcccgc
    25021 tgggcgcggg ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc
    25081 caatcctgcg aaacttatct tattcagaat acgcgcccgg aattgatcac tgatactttc
    25141 cgcgaatttt ttgacacgcc ggtagaaacc gcgcaggacg tccacaagca gctcaaacgc
    25201 ctgcggagag ttatcgcctg gactggggaa cgggaaacgc ctgtcaccat ttatagctgg
    25261 gtcgcggcgg caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc
    25321 aacgccaccg aagaagagat cctgcaaggc gaaccggaag taaaagtaga gtcagccgaa
    25381 cgtcatcatg caatggttaa cttctggcga accacacttt cctgcattct gggcacgctt
    25441 ttctggctgt ggacgggctg gacttccggc agtggtgcaa tggtgatgat tgcggtcgtg
    25501 acgtcactgg caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctatggg
    25561 acgctggccg cgctgccgtt agggctgctc tactttttgg tgattatccc taatacccaa
    25621 cagagcatgt tgctgctgtg tattagcctg gcagtgctgg gattcttcct cggtatagaa
    25681 gtacagaaac ggcgactggg ctcgatgggg gcactggcca gcaccataaa tattatcgtg
    25741 ctggataacc cgatgacttt ccatttcagt cagtttctcg acagcgcatt aggacaaatc
    25801 gtcggctgtg tgctcgcgtt caccgttatt ttgctggtgc gggataaatc gcgcgacagg
    25861 actggacgtg tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat
    25921 gtggcacgtc gtaaagagaa ccacctcccg gcactttatc agcagctgtt tttgctgatg
    25981 aataagttcc caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcgcac
    26041 cagcgcctgc gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa
    26101 atgcgccgca cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac
    26161 tttggccagt tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagcg
    26221 ccaccgcagg tgacggaacc ggttcatcgg ctggcgggga tgctccataa gtatcaacat
    26281 gcgttgaccg atagttaagt caaaaccgac gccaaaagcg tcggtttttt catggctata
    26341 cttagcgatg cacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta
    26401 tgcctttgat tttgatgaga ttgagagtca ggaggatttt tatcgtgact ttagccaaac
    26461 ctttggtctg gctaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa
    26521 cgatgtcctg ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg
    26581 ccgttttggc gcgttaatat tgctgtttga tgaggcagag gaagagctgg aagggcattt
    26641 gcgttttaat gttcgtcatt agcgcaaaaa aaagcccacg aaccgggggc aatatcgtcg
    26701 gacaaaacga tgagggttta tttgtacagt tcagccgtag cgtgccaggt gtcaccgcta
    26761 cgagcttcag taatctggta ggccgttgcg cctttctctt ccgctttttt gttcagcatt
    26821 tcacgtatat ccattggcga agacgccaca ccacttacgg atacggtccc gattgcttca
    26881 cgattttgtg cttgtgcagc atcaatggag tcggcagcga atgcaccgaa agagagaaca
    26941 gaaagtacgc ttaatgcagc tacagtggtt ttgattttca tgatttttac ctcgacataa
    27001 tcttttagct gggcctttgt ttcgtgaccc atatcacaaa atcaagtata cactaattat
    27061 tgaactaatt aataccacac taactgtttc agttgatact aagtgttaaa aatgtgaatt
    27121 ttaataacaa aaaagtataa aaaacacggt tatttattat taattatagt attttcaatt
    27181 agataatgaa ttttgcataa tgtgcttttt ttaccgtatg tattcatttt gtgaatgatg
    27241 tgtcgcggta aaacgcacta ttcgttaaat gtatcgcagg agaaagaagg ggaatgagag
    27301 ggataagcaa cattttcccc gccgccagaa gcgacggggc agagattaaa gctcctggtc
    27361 gaacagctct aaaatcgctt cgtacaggtc tttgacggtg aaaccgttag caggggtggt
    27421 aaagatggtg tcatcgccag cgatggtgcc cagaatacct tctgctttgc ccagtgagtc
    27481 cagcaggcga gcaattaact gcgccgcgcc agggctggta tgaatcacga caactgcatc
    27541 gttgtagtcg atatccagca ccagattctt caatggactg gaggtggttg gtacacccag
    27601 ttcagctggc aggcagtaaa ccatttccat tttggcattg cgtgtacgta cagcaccaaa
    27661 cttggtcaac atccgcgaga ctttagactg attaatattg tcaaagcctt gctcctgcaa
    27721 cgcggcgacg atttcgccct gggagctaaa tttctcttct ttaagtaatg ctttaaatgc
    27781 tttaactagt tcttcttgct tagccgagct tcgcataagt cacccgatat ggtggttgat
    27841 acaacattat tgtgcataca gatgaatttt tatgcaaaca gtcagccctg aagaaggctg
    27901 aaataatgtt atgaaagagc gggattttat caaatttcgt tattgagaaa catgcctgcg
    27961 tcacggcatg caaattctgt ttaaaagtaa attaattgtt atcaaattga tgttgttttg
    28021 gctgaacggt agggtatatt gtcaccacct gttggaatgt tgcgctaatg cataagcgac
    28081 tgttaattac gtaagttagg ttcctgatta cggcaattaa atgcataaac gctaaacttg
    28141 cgtgactaca cattcttgag atgtggtcat tgtaaacggc aattttgtgg attaaggtcg
    28201 cggcagcgga gcaacatatc ttagtttatc aatataataa ggagtttagg atgaaagtcg
    28261 cagtcctcgg cgctgctggc ggtattggcc aggcgcttgc actactgtta aaaacccaac
    28321 tgccttcagg ttcagaactc tctctgtatg atatcgctcc agtgactccc ggtgtggccg
    28381 tcgatctgag ccatatccct actgctgtga aaatcaaagg tttttctggt gaagatgcga
    28441 ctccggcgct ggaaggcgca gatgtcgttc ttatctctgc aggcgtagcg cgtaaaccgg
    28501 gtatggatcg ttccgacctg tttaacgtta acgccggcat cgtgaaaaac ctggtacagc
    28561 aagttgcgaa aaactgcccg aaagcgtgca ttggtattat cactaacccg gttaacacca
    28621 cagttgcaat tgctgctgaa gtgctgaaaa aagccggtgt ttatgacaaa aacaaactgt
    28681 tcggcgttac cacgctggat atcattcgtt ccaacacctt tgttgcggaa ctgaaaggca
    28741 aacagccagg cgaagttgaa gtgccggtta ttggcggtca ctctggtgtt accattctgc
    28801 cgctgctgtc acaggttcct ggcgttagtt ttaccgagca ggaagtggct gatctgacca
    28861 aacgtatcca gaacgcgggt actgaggtgg ttgaagcgaa agccggtggc gggtctgcaa
    28921 ccctgtctat gggccaggca gctgcacgtt ttggtctgtc tctggttcgt gcactgcagg
    28981 gcgaacaagg cgttgtcgaa tgtgcctacg ttgaaggcga cggtcagtac gcccgtttct
    29041 tctctcaacc gctgctgctg ggtaaaaacg gcgtggaaga gcgtaaatct atcggtaccc
    29101 tgagcgcatt tgaacagaac gcgctggaag gtatgctgga tacgctcaag aaagatatcg
    29161 ccctgggcaa agagttcgtt aataagtaat tgattagcga ataataaaaa accggagcac
    29221 agactccggt ttttgttttg agcactcgac ttaattggtt gccggatatt cctgaatggt
    29281 gacctgcagc gttaactgct tatcatcacg catcactaca accgggatca ccgaaccagg
    29341 gcgaatttcc gccacctgat ccatcgtctc cagagcagag atggccggtt tgttatccac
    29401 cgaaataatc agatcgttga cctgaatacc cgcattcgcc gctgggccgt caggtgacac
    29461 ttcattaacc acgatccctt gcagttgatc tataccaccg ctctgcgcgt gcagtggtgc
    29521 gatctcgcgt ccgccgatac caatgtagcc gcggatcacg cgaccatcgc ggatcagctt
    29581 atccataatt ttggttgcta actggaaagg aatcgcaaag ccgatacctt ccggcgtttc
    29641 gccatcgtta ctcttatcaa acgacagcgt gttaatgccc atcagttctc ccagcgagtt
    29701 caccagcgcg ccgccagagt taccgtggtt aatggaagca tcggtttgta ggaagttttg
    29761 ccgcccggtc gggttcagac cgattcgacc cgtggcacta ataatccctt gggtaatggt
    29821 ctgcccgagg ttgtacgggt taccgatcgc cagtactacg tcgccaatgt gcggtacgcg
    29881 acgtgcatta attggaatgg taggtaaacc gccagtggca ttaattttaa gtaccgccag
    29941 atcggttaga gagtcagatc ccaccagcaa tgcttcaaat acacgtccat cctgtaaggc
    30001 gacgatgatc tgatcggcgt cgttgatgac gtgtttattg gtgatgatat aaccgcgttg
    30061 atccatgatt acaccggatc ccagggtgcg gatctcaagc tggttgtgag agttggtgtt
    30121 caaaccacgg ttgtaaacgt taaccaccgc tggcgcggcg cggcgaaccg ccagattata
    30181 gctggcaggc gtctcatcgg tactgtcaaa ttgcggagtg gaaagcgggt taaggctgcg
    30241 cagcgaaggc atggcaacca gcagaatagc gccgacaatt aatccaatcg caacggaacg
    30301 taagagcttc acaaacatga tggaggcgtc attaaaaaag ggaacggcag cagcatacca
    30361 cgagttaacc ggacatcaca cgtcagcctg atgcccggtt tacgacatta acgcatcagc
    30421 agatagatgc tttcattgcc gcgtacaatt tgcagggcga tgatggccgg ttttgccgcc
    30481 agcactttac gcatttcagc aatcgagttc acccgatcgc ggttgacgcc aatgatcaca
    30541 tcgtcttttt gcaagccagc ctgagcagct gggcttcctt tgacaacttc atctatctta
    30601 atacctttgc cgccatcttt tagctgacca tcgctcaacg ttgcaccttc cagcgctggc
    30661 cgacgaagag gtgctggtat caagcgtcac ttctacttcc agtggtttgc cgttacgcag
    30721 caggccaagc ttcactttcg tgcccggctc tgtagtcgcg atacgagagc gcaactcagc
    30781 aaagctattc agcggtttgc cgttgaggct ggtaataata tcgcccgctt tgacgcccgc
    30841 tttcgctgag ccagaacttg gcaacacttc gctgacaaac gcgccacgct gcacgtcaag
    30901 gttgaaggct ttggcgatat cggcactcat ctcggtgcct ttgatgccta acaaaccgcg
    30961 tttgatttca ccaaagtcga taagctgctg cgccagtgtt cgcgccatat tactggggat
    31021 ggcaaatcca atcccgacgc tcccgccgcc aggcgcaagg attgcagtgt tgatgccaat
    31081 taactcaccg ttaaggttta atagtgcacc gccggagtta ccgcggttaa tggaagcatc
    31141 tgtctggata aagttttcca gaccttcaag attcaacccg ctgcggccta atgcggaaac
    31201 aatgccagag gtggcggttt gcccaaggcc aaatgggtta ccgaccgcta cggcaaaatc
    31261 accgacgcgc aatttatcgg agtcggcaat agcgatttgc gttaatttgc tcgggttttg
    31321 aatttgtaac agggcgatat cgctctggtc atcgctacca atcagttttg catcaaactc
    31381 gcgcccatca ttgagctgaa tactgatttt ctgtgcctga ttaatcacat ggttgttggt
    31441 cagcacatag cctttactgg cgttgatgat gacaccggaa cctaaacctt cgaagggttg
    31501 tgcaggttga tccggtaaat catcaccaaa aaactttttg aattcttccg ggattttctg
    31561 tccctgactg gccgttcctt ccacccgtac gctcaccact gccggaagca ctttttccag
    31621 cattggagcc agactgggga gaggggcctg atcggcaacc tggcctggaa tcgacgcgac
    31681 ggcctgaaat gacgccgaga gagttaaccc ggcacttaac gctaatgcac tcaacagctg
    31741 ggtttgtttt ttcattattc ctgctctcgt acctgaatga taagaaaaga gattcaaaac
    31801 agtttgatgt tattgaattt tcagacggct aacaatgaga caccagatta aatgatagtc
    31861 ggggagggag agaagaggag ggcgcaatgg ctgcgcccga aaaataaatt agtcgcgctt
    31921 cgcgccagta cgcagcaggc cggatgcgcc ttcagaatag tcgcgaggca cctgcaccgg
    31981 tgcctgatcg ttgctggctt cagactctgc cagacgatta cggaacgggt ttgcttcagc
    32041 agacagttcc ggcagcaggc tgctggagct ttttgccatg tgctgataca gctggcgata
    32101 gtcgtgcgcc atggtatcca gtaattccgc gctgcgggca aagtggctaa ccagctcttc
    32161 gcgatactcg tccagttcag ctttattctt ttccagttcg tactgcaacg cctgttgctg
    32221 gcgtagttta cgattaccaa aacgcatggc cacagcacca ataatgatgc cgacgactaa
    32281 cccaattagc gcatattccc aggtcatgaa catctcccgt tgtcttttgt ttccgtaggg
    32341 tgttggcttc aggctccatg cctgcggctg attatgccac tataaccgtt aattccacag
    32401 aagtggaatc ccgactgcat atcgcgtagt gtagaacggc ctttttttcg tcaaccgtga
    32461 acaacggcgc accgattatt caaggaataa caataagatc atgcaaagcg ttaccccaac
    32521 atcgcaatac ctgaaggcgc tcaatgaagg cagccatcaa cccgacgacg ttcaaaaaga
    32581 ggccgtcagc cgcctggaaa ttatttatca agaactcatc aatagcaggc caccagcccc
    32641 caggacgagt gggctaatgg cgcgggtcgg taagctgtgg ggtaaacgcg aagacacaaa
    32701 gcatacgcca gtgcgtggct tatatatgtg gggcggtgta ggacgcggga aaacctggct
    32761 gatggacctt ttctatcaaa gcctgccggg agagcggaaa cagcgcctgc actttcaccg
    32821 ttttatgctg cgggtgcacg aagagctaac tgccttacag gggcagaccg atccgctgga
    32881 aattattgcc gatcgcttta aagccgaaac tgacgtgctc tgttttgacg aattttttgt
    32941 ttctgatatt accgacgcca tgctacttgg cggtctgatg aaagccctgt tcacccgcgg
    33001 tattaccctg gtagcgacgt caaatattcc gccggacgaa ctttatcgaa atggcctgca
    33061 acgtgcgcgt tttctgcctg caatcgatgc cattaaacag cattgtgatg taatgaacgt
    33121 ggacgctggt gttgattatc gactgcgtac actcactcag gcgcatctgt ggctttcgcc
    33181 actcaacgat gaaacccggg cgcagatgga taaactatgg ttggcgctgg cgggggtgaa
    33241 acgagaaaat tcaccgacgt tagaaatcaa ccatcggcca ttggcgacaa tgggcgtcga
    33301 gaaccagacg ctggcggttt cttttactac gctgtgcgtc gacgcccgca gtcagcatga
    33361 ctatattgcg ctctcacgcc tgtttcacac agtaatgttg tttgatgtgc cagtcatgac
    33421 gcggttgatg gagagcgaag cgcggcgctt tattgcgctg gtggatgagt tttacgagcg
    33481 ccatgtcaaa ttagtggtga gtgcagaagt gccgctgtat gaaatttatc agggtgagcg
    33541 gctgaagttt gagttccagc gttgcctgtc acgtctgcaa gagatgcaaa gcgaagagta
    33601 tctgaagcgc gagcatttgg cgggttaaaa cctgccacaa atcacaaaaa ggggtcgatc
    33661 tttgaccccg acttctctat aatcctgcga ccccacgtta caagaaagtt tttttcccaa
    33721 aactttttgt gtgctggcat aggctattcg aaggggtagg tttgccggac tttgtcgtgt
    33781 gaacctcaac aattgaagac gtttgggtgt tcaccaacgt gtaactattt attgggtaag
    33841 cttttaatga aaacttttac agctaaacca gaaaccgtaa aacgcgactg gtatgttgtt
    33901 gacgcgaccg gtaaaactct gggccgtctg gctactgaac tggctcgtcg cctgcgcggt
    33961 aagcacaaag cggaatatac tccgcacgta gataccggtg attacatcat cgttctgaac
    34021 gctgacaaag ttgctgtaac cggcaacaag cgtactgaca aagtgtacta tcaccacacc
    34081 ggccacatcg gtggtatcaa acaagcgacc tttgaagaga tgattgctcg ccgtcctgag
    34141 cgtgtgattg aaatcgcggt taaaggcatg ttgccaaaag gcccgctggg tcgtgctatg
    34201 ttccgtaaac tgaaagttta cgcgggtaac gagcacaacc acgcggcaca gcaaccgcaa
    34261 gttcttgaca tctaatcggg attataggca atggctgaaa atcaatacta cggcactggt
    34321 cgccgcaaaa gttccgcagc tcgcgttttc atcaaaccgg gcaacggtaa aatcgtaatc
    34381 aaccaacgtt ctctggaaca gtacttcggt cgtgaaactg cccgcatggt agttcgtcag
    34441 ccgctggaac tggtcgacat ggttgagaaa ctggacctgt acatcaccgt taaaggtggt
    34501 ggtatctctg gtcaggctgg tgcgatccgt cacggtatca cccgcgctct gatggaatac
    34561 gacgagtccc tgcgttctga actgcgtaaa gctggcttcg ttactcgtga cgctcgtcag
    34621 gttgaacgta agaaagtcgg tctgcgtaaa gcacgtcgtc gtccgcagtt ctccaaacgt
    34681 taattggctt ctgctacggc agaaaacaat ttgcgaaaaa acccgcttcg gcgggttttt
    34741 ttatggctaa attctgaatc agcgtaaaaa atgggaagtt gctttttgct gccgtctgaa
    34801 agacaggtaa aacaaaaacc acatcgccaa taagggtcta agtcaactat ttcagactaa
    34861 agcgcatctc tttttcccca tttccggcat ccactcacca caaaggtcgc aaaatctggt
    34921 aaactatcat ccaattttct gcccaaatgt cgggtattgc tcattttttg tttgattttc
    34981 gaacaaagag agtcgttcct tattgggtaa cacaacttct gactggccac ctggtggctg
    35041 gtagcagtaa aaattctgac tatacctgga ggttttcatg gctgtcgctg ccaacaaacg
    35101 ttcggtaatg acgctgtttt ccggtcctac tgacatctat agccatcagg tccgcattgt
    35161 gctggctgag aaaggtgtaa gtttcgagat cgaacacgtg gaaaaggaca atccgcctca
    35221 ggatctgatt gacctcaacc cgaatcagag cgttccgacc ctggtggatc gtgagctgac
    35281 cctgtgggaa tctcgcatca ttatggaata tctggatgag cgtttcccgc atccgccact
    35341 gatgcctgtt tacccggtag ctcgcggtga aagccgtctg tacatgcatc gcatcgaaaa
    35401 agactggtac acgctgatga acactatcat caacggttca gcttctgaag cagatgccgc
    35461 acgtaagcaa ctgcgcgaag aactgctggc gattgcgccg gtcttcggtc agaagccgta
    35521 cttcctgagc gatgagttca gcctggtcga ttgctacctt gctccgctgc tgtggcgtct
    35581 gccgcaactg ggcatcgagt tcagcggccc gggtgcgaaa gagctgaaag gctatatgac
    35641 ccgcgtcttt gagcgtgact ctttccttgc ttctttaact gaagcagaac gtgaaatgcg
    35701 tctgggccgg agttaatctg tatggatttg tcacagctaa caccacgtcg tccctatctg
    35761 ctgcgtgcat tctatgagtg gttgctggat aaccagctca cgccgcacct ggtggtggat
    35821 gtgacgctcc ctggcgtgca ggttcctatg gaatatgcgc gtgacgggca aatcgtactc
    35881 aacattgcgc cgcgtgctgt cggcaatctg gaactggcga atgatgaggt gcgctttaac
    35941 gcgcgctttg gtggcattcc gcgtcaggtt tctgtgccgc tggctgccgt gctggctatc
    36001 tacgcccgtg aaaatggcgc aggcacgatg tttgagcctg aagctgccta cgatgaagat
    36061 accagcatca tgaatgatga agaggcatcg gcagacaacg aaaccgttat gtcggttatt
    36121 gatggcgaca agccagatca cgatgatgac actcatcctg acgatgaacc tccgcagcca
    36181 ccacgcggtg gtcgaccggc attacgcgtt gtgaagtaat acaaaacagg cccaggcggc
    36241 ctgttttgtc tttttaatga cttaataaaa tagcgtgatt gtggcaatca tattcaccac
    36301 gaaaccgact gccatgggga tcgctgtccg cttcaccacc gcaaaaggcg aaacgcctgc
    36361 aattcctgac gtaacgacca ccaccgcagt aatcggcgaa accgcgcgcg ctagcgtcgt
    36421 ggcaaaatgc atcggcataa tcattacaac cgctggtaca tgtagtccgg ctgcgatatt
    36481 cggaataaga ctggcaaatg acataaacgg cgcattgcca gagcccatca caatggcaca
    36541 aatggcaatg actagcgcca taataatcat cacgccaata ccgcccagac cagaatgctc
    36601 cgcacccctg ataaccgcat caacagtgcc aatcgtcgtt aagcctttcg caaatatttc
    36661 acccgcgacc accagcgtta ccacgttgac aaactgcgta cccatgccgt caaaaaacgc
    36721 ctgcacatcg tccattgttt cgcgcaagtt atgcttgcgg aagaactcaa caaacatcgt
    36781 caccgtcaaa cttagtaaca tcaccaccac cagatgcagt tctgactgca tcagcccgac
    36841 gtgggcgagg aacagcgagc cgagcatcag gattaacggc atcacaggta aaatggcgta
    36901 atagagcggc gggacattat cgagagtttt ttgctctgcc tgttcgtgat tgatattttt
    36961 atcttttttg tcaaaagcgc gttgcacgaa aaagtgggag atcgccaccg agataatgac
    37021 gcaagaggcg accggaagct ggtagtggaa gaagtatgtg gcaattttca ttcccgcgac
    37081 ctgggctgca aaaatggagt tcgtttccag aatcccccac tcaatggaca tcgtggttgc
    37141 gataaccgcc accgcagaga ggcgacttac tcccagactc accagcgtcg gaaataaggt
    37201 gaccatcagc aacataccca gaccggaggc gctggtaata aactgcgcca tgatttggcc
    37261 gatgacgtaa gttgccgaca gaataatata cggcgagcga atgagtttta acgggcggct
    37321 tagcaagctc accatcgcgc aactggctcc tatgcgctcc atgtagcggg cataaccgcc
    37381 caccgccata atcgacagcc ccaatccggc aatgcggttg gacagcatgc gcaggatttc
    37441 attataaatg tcgacgatca agtagccgct gcttttggcg ggatcgagta caccaccgaa
    37501 ccctaaccag actccgcaca tcatcaggaa gacacctgcg gcagccagca ccacctgagg
    37561 tttgtagttt ttcaggatca aatagcccat cgtaattaat acgatgacag atataattat
    37621 gccgaacatt g
//
LOCUS       DAJTRM010000029        35566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRM010000029 DAJTRM010000000
VERSION     DAJTRM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.029"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(100..858)
                     /gene="deoR"
                     /locus_tag="OO322_001593"
     CDS             complement(100..858)
                     /gene="deoR"
                     /locus_tag="OO322_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5906519.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(916..1512)
                     /gene="ybjG"
                     /locus_tag="OO322_001594"
     CDS             complement(916..1512)
                     /gene="ybjG"
                     /locus_tag="OO322_001594"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5906520.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1797..3023
                     /gene="mdfA"
                     /locus_tag="OO322_001595"
     CDS             1797..3023
                     /gene="mdfA"
                     /locus_tag="OO322_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5906521.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3064..3348)
                     /locus_tag="OO322_001596"
     CDS             complement(3064..3348)
                     /locus_tag="OO322_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906522.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3434..4249)
                     /gene="ybjI"
                     /locus_tag="OO322_001597"
     CDS             complement(3434..4249)
                     /gene="ybjI"
                     /locus_tag="OO322_001597"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5906523.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4249..5457)
                     /locus_tag="OO322_001598"
     CDS             complement(4249..5457)
                     /locus_tag="OO322_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5906524.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5541..6077
                     /gene="rcdA"
                     /locus_tag="OO322_001599"
     CDS             5541..6077
                     /gene="rcdA"
                     /locus_tag="OO322_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5906525.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6252..7937)
                     /locus_tag="OO322_001600"
     CDS             complement(6252..7937)
                     /locus_tag="OO322_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5906526.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8207..8584
                     /locus_tag="OO322_001601"
     CDS             8207..8584
                     /locus_tag="OO322_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5906527.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8614..8871)
                     /gene="grxA"
                     /locus_tag="OO322_001602"
     CDS             complement(8614..8871)
                     /gene="grxA"
                     /locus_tag="OO322_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5906528.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9031..9318
                     /locus_tag="OO322_001603"
     CDS             9031..9318
                     /locus_tag="OO322_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5906529.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9302..10024
                     /gene="nfsA"
                     /locus_tag="OO322_001604"
     CDS             9302..10024
                     /gene="nfsA"
                     /locus_tag="OO322_001604"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5906530.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10085..10987
                     /gene="rimK"
                     /locus_tag="OO322_001605"
     CDS             10085..10987
                     /gene="rimK"
                     /locus_tag="OO322_001605"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5906531.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11075..11551
                     /locus_tag="OO322_001606"
     CDS             11075..11551
                     /locus_tag="OO322_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5906532.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11903..13015
                     /gene="potF"
                     /locus_tag="OO322_001607"
     CDS             11903..13015
                     /gene="potF"
                     /locus_tag="OO322_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5906533.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13110..14243
                     /gene="potG"
                     /locus_tag="OO322_001608"
     CDS             13110..14243
                     /gene="potG"
                     /locus_tag="OO322_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5906534.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14253..15206
                     /gene="potH"
                     /locus_tag="OO322_001609"
     CDS             14253..15206
                     /gene="potH"
                     /locus_tag="OO322_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5906535.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15203..16048
                     /gene="potI"
                     /locus_tag="OO322_001610"
     CDS             15203..16048
                     /gene="potI"
                     /locus_tag="OO322_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5906536.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16108..16596
                     /locus_tag="OO322_001611"
     CDS             16108..16596
                     /locus_tag="OO322_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5906537.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16637..17764
                     /gene="rlmC"
                     /locus_tag="OO322_001612"
     CDS             16637..17764
                     /gene="rlmC"
                     /locus_tag="OO322_001612"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5906538.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17939..18670)
                     /gene="artJ"
                     /locus_tag="OO322_001613"
     CDS             complement(17939..18670)
                     /gene="artJ"
                     /locus_tag="OO322_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5906539.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18962..19630)
                     /gene="artM"
                     /locus_tag="OO322_001614"
     CDS             complement(18962..19630)
                     /gene="artM"
                     /locus_tag="OO322_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5906540.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19630..20346)
                     /gene="artQ"
                     /locus_tag="OO322_001615"
     CDS             complement(19630..20346)
                     /gene="artQ"
                     /locus_tag="OO322_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5906541.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20353..21084)
                     /gene="artJ"
                     /locus_tag="OO322_001616"
     CDS             complement(20353..21084)
                     /gene="artJ"
                     /locus_tag="OO322_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5906542.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEIITVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21102..21830)
                     /gene="artP"
                     /locus_tag="OO322_001617"
     CDS             complement(21102..21830)
                     /gene="artP"
                     /locus_tag="OO322_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5906543.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22048..22563)
                     /locus_tag="OO322_001618"
     CDS             complement(22048..22563)
                     /locus_tag="OO322_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5906544.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23212..24981
                     /locus_tag="OO322_001619"
     CDS             23212..24981
                     /locus_tag="OO322_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5906545.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25192..25515
                     /locus_tag="OO322_001620"
     CDS             25192..25515
                     /locus_tag="OO322_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5906546.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25512..26342
                     /gene="amiD"
                     /locus_tag="OO322_001621"
     CDS             25512..26342
                     /gene="amiD"
                     /locus_tag="OO322_001621"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5906547.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26339..27352)
                     /locus_tag="OO322_001622"
     CDS             complement(26339..27352)
                     /locus_tag="OO322_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5906548.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27451..28881)
                     /locus_tag="OO322_001623"
     CDS             complement(27451..28881)
                     /locus_tag="OO322_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5906549.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28892..29893)
                     /gene="ltaE"
                     /locus_tag="OO322_001624"
     CDS             complement(28892..29893)
                     /gene="ltaE"
                     /locus_tag="OO322_001624"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5906550.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29930..31648)
                     /gene="poxB"
                     /locus_tag="OO322_001625"
     CDS             complement(29930..31648)
                     /gene="poxB"
                     /locus_tag="OO322_001625"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5906551.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31781..32749)
                     /gene="hcr"
                     /locus_tag="OO322_001626"
     CDS             complement(31781..32749)
                     /gene="hcr"
                     /locus_tag="OO322_001626"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5906552.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32761..34413)
                     /gene="hcp"
                     /locus_tag="OO322_001627"
                     /gene_synonym="priS"
     CDS             complement(32761..34413)
                     /gene="hcp"
                     /locus_tag="OO322_001627"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5906553.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34557..35456)
                     /gene="lysO"
                     /locus_tag="OO322_001628"
     CDS             complement(34557..35456)
                     /gene="lysO"
                     /locus_tag="OO322_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5906554.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8861 a         8797 c         9133 g         8775 t
ORIGIN      
        1 gggatttcgt gatgatgaaa ttccatcagt ggttcggcag ctggttctct taatcttctg
       61 ataaccggat ggcgcgaaac gtcatccggt tatacgtcat taatacatca acttaatacg
      121 gtgcgtctgc gcgtacttca catactcatc ttccgggcaa caatcactca ccacaatatc
      181 aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg ccaaatttac tgtggtcgac
      241 aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt tttaccggca actcttcaag
      301 attaaaacag gtagcgcctt tactgacatg cacgcccgcc gcggaataaa aagcgatatc
      361 cgggcaaaaa ttattcaacg tttgctgaaa atcgatgggt ttgaaaatgg cgttgctggc
      421 gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt ttctctttca gcgccagaaa
      481 ggtatttagc gaataacaaa cggcggtaaa agggatttca ttatcaatcg cttcaataat
      541 ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga tcgggttcta ccagtgtcgc
      601 agccagtttt gccgcccggc gtttttcttc caccaggcgg gatttttgat cgcttaacag
      661 gtaatggctg gcactgcgcg gttccagaac aatatagccg ccgagcaaaa cgacgggcgc
      721 actgtggttg ttcagatcgc gacgaatcgt catctccgaa accccaagca gggcggcggc
      781 gtctttaaga tgtaacttat cgctgcgttt taattcttgc agcagctgcc cgatacgctc
      841 ttcgcgacgt gtttccataa tccctctgaa tagttattga agcgagccgc tcaatactac
      901 actttttagc agagatcagt cacgcaccca gcctttgcgg atcggtaatg caaaacagac
      961 gcgataccac gattgcagac gttgatagag tttatgcccc atcgcttgcc agataatctg
     1021 ggcactaagg cagccaatca tacctgccag caatccaccg agcatatcca gcggccagtg
     1081 gacgccaaga taaacgcgcg accaggcaat gacgacggcc agcaccatta aaagtgagcc
     1141 ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag gtgaaaatca ccgtaccgtg
     1201 atcgctcggg aatgagtcat ccgccgcatg atgcaggaag ttatagccga tattttcgac
     1261 aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag gacacaaaca ggctgaccgc
     1321 cagcgcgata gcgattttta tcaccagttg ccgttgtgct gtaagccccc acaaccaaag
     1381 taccgcggcc agcaacggca ccacggtaat caaatcttta gcaataaaaa tcgccaacga
     1441 gatcatccac ggagccgagt ctggcgtcgc gttaataaga gagaatagag agagattcaa
     1501 attttccagc ataacttccc gacgcaaagt gattaaaagg gagccaatac aggcaagtcg
     1561 ttgagaataa agtgcaggtt aaactgggta aagcggcatc gtcttatttc cctcaagcgg
     1621 cctgtttacg gtgggtgatt gtaacgggca taggttaaat aaaacttaaa gaaagcgtag
     1681 ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt tcagctacaa aattcgcttt
     1741 ctcgttagct gcgcttttat taaactctgc gcgattatta ttggcgaaga aattgcatgc
     1801 aaaataaatt agcttccggt gccaggcttg gacgtcaggc gttacttttc cctctctgtc
     1861 tggtgcttta cgaattttca acctatatcg gcaacgatat gatccaaccc ggtatgttgg
     1921 ccgtggtgga acaatatcag gcgggcattg attgggttcc tacttcgatg accgcgtatc
     1981 tggcgggcgg gatgttttta caatggctcc tggggccgct gtcggatcgt attggtcgcc
     2041 gtccggtgat gctggcgggt gtggtgtggt ttatcgtcac ctgtctggca atattactgg
     2101 cgcaaaacat tgaacaattc accctgttgc gcttcttgca gggcataagc ttctgtttca
     2161 ttggcgctgt gggatacgcc gcaattcagg aatccttcga agaggcggtt tgtatcaaga
     2221 tcaccgcgct gatggcgaac gtggcgctga ttgctccgct acttggtccg ctggtgggcg
     2281 cggcgtggat ccatgtgctg ccttgggagg ggatgtttgt tttgtttgcc gcattggcag
     2341 cgatctcctt tttcggtctg caacgagcta tgcctgaaac cgccatgcgt ataggcgaga
     2401 aactgtcact gaaagaactc ggtcgtgact ataagctggt gctgaagaac ggccgctttg
     2461 tggcgggggc gctgggattc gttagcctgc cattgctggc gtggatcgcc cagtcgccga
     2521 ttatcatcat taccggggag cagttgagca gctatgaata tggcttgctg caagtgccta
     2581 ttttcggggc gttaattgcg ggtaacttgc tgttagcgcg tctgacctcg cgccgcaccg
     2641 tacgttcgct gattattatg ggcggctggc cgattatgat tggtctgttg gtcgctgctg
     2701 cggcaacggt tatctcatcg cacgcgtatt tatggatgac cgccgggtta agtatttatg
     2761 ctttcggtat tggtctggcg aatgcgggac tggtgcgatt aaccctgttt gccagcgata
     2821 tgagtaaagg tacggtttct gcggcgatgg gaatgctgca aatgctgatc tttaccgtcg
     2881 gtattgaaat cagcaaacat gcctggctga acgggggcaa cggactgttt aatctcttca
     2941 accttgtcaa cggaattttg tggctgtcgc tgatggttat ctttttaaaa gataaacaga
     3001 tgggaaattc tcacgaaggg taaaaaaatg cctgattgat ttgtgcgatc aggcattctc
     3061 gaattaatgg tgatggtcgt caatctggtg ttcgataacc atcccttcac ctacgctggc
     3121 aagatggcga acataaggat gcgggcggta agccggagct ggcgcaggag ccacatagac
     3181 ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg ctgactgaat caggaaccac
     3241 aaccactttg tatccactcg gcacatcgac ggtgacactt tgcgccatcg ccacaccagt
     3301 aaagcccatt aaaagcgcgc ccaacagtag acaatttttc ataatcattt cgcctgaaat
     3361 aatgcagcgg gttaatttcc gcgaattatg cagccttcat tcaggcattg tgttgccggg
     3421 cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt aagaacttta tcgatcacat
     3481 ccagtacgcc ttcacggtta ttgtagcctg cccggtattt tgccgctgcg acgactgcgc
     3541 tgccggcatt ttccattgca aaactaaagc ctgcctgacg cagcatctca atatcgttac
     3601 cgccatcgcc aaagaccacc acttcgctgt cgtctattcc ccataatttc tgcagttggc
     3661 gaaggccatt ggctttatgt acgccgggga taatcagatc gatgctgccg ttgccggtgt
     3721 ggactggcac cataatatcg ccgatggcct catgtaatgc tttttgtact tgtggaatca
     3781 gttcatcgga aagattcagg ccaaacttaa agaaaatatc ttctaagttg tcaaagttat
     3841 cgacgtattc cagacggtga taatacattt ccgccaccgt tttcatggca tcgtcatatt
     3901 ttttgagtgt ataggcgcta ttttttccgc aggcaataat ttccacttcc gggcgcgtca
     3961 gcaaatgttc cacgacagta gcaaacgcat ccttcgacag ctcgccatta aaaacatctt
     4021 tgccttcact cattacccag ccgccgtttt cagccacaaa ggcaatttca ttcgcaattt
     4081 cagggaagaa agagatcaac tgataatatt gattcccgct ggcgaccaca aagcgaatgc
     4141 cttgtgcttt catttgctga tactgagcca taaaccgctc acggttatag gttttttgat
     4201 cgcttaagaa agtaccatcc atgtctaccg caattaattt aatgctcatc aactattctc
     4261 catcgccgtc tgcgttttgg tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag
     4321 aataaccagc gccagtacaa ccagcattgc actacgtaat ccataatgtt cgccgagata
     4381 gcccagcagc ggcggcccga cgaggaaagc cagataaccg gtcgttgcta ccacactgac
     4441 gcgggttggt gcatcggggc cggtatcgct ggcggcagaa atggtcagcg ggaagcccag
     4501 cgaggcaccc agtccccaaa gtacaacaga cactcctgcg acccaggcgc tatcgacaaa
     4561 aataatcagc ccaataccca acgcccccat tagtgcactg gcccgaacca cggcaacgcg
     4621 actgtaactg tcgatgaacc aaccgccggt gaagcgtcca acggtcatcc ccagggtaaa
     4681 accggcataa atcagtgagc cggaagtggg gctaaaaccg tgaccatcaa ccattaataa
     4741 gggtaaccag tcgttggcag aaccttcggc aaaggccatc gccagcacta caacaccaat
     4801 caacagcaac tggatatcgc gataaaaagg aatgcctttt tcgccatgct gggtgccatc
     4861 ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg gcgatataaa taggcgcgat
     4921 acctaccagc gccgccaata aaatgtgcac cgttgccgga acgccaaagg ccgtcagtgc
     4981 catcccgaca ccagcgcctg ccagcgtgcc caggctataa aaaccgtgca tcatcggcaa
     5041 aaccgtttta ttcatttctc gctcaacggc ggcaccttca acgtttatcg ccacttccgc
     5101 agaaccaaaa cttgccccaa agacgccgag accaacggca aagagcaggg gcgatgtcag
     5161 ccagagtgcc agacttaata tcatcatccc gatcaatgcg caggacatcg tgaccaggat
     5221 gacattacgt gtcccaaagc gtttcactaa ccacgccgag cagagaatac cgctcatcga
     5281 accgatcgac agaccaaaga gaacaccgcc catttcagcg atcgagacag agagaatatc
     5341 gcggatagca ggcgtacggg ttgcccagga cgccattaac aggcctggca aaaagaagaa
     5401 cataaacagc gcccaggttc ggcgtttcaa tgcattacgt gaagaattta cggtcataga
     5461 tcacgtcaaa ataagaagag ggaagacaac attagcaagg ttgtgtacat ttgtacacaa
     5521 ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta
     5581 tccaggccac gctggaggcg gtgaaacttt acggaataca tgctgttacg caccgcaaaa
     5641 ttgctaccct tgccggggta ccgttgggat cgatgaccta ctatttttca ggaattgatg
     5701 agttgttgct ggaggcgttc agcagtttta ctgagattat gtcccggcaa tatcaggcat
     5761 tttttagcga tgttagtgat gctcaaggcg catgccaggc tatcaccgat atgatctaca
     5821 gctcacaggt tgcaacgccg gataatatgg agctgatgta ccagctctac gcgctggcta
     5881 gccgaaaacc gctattaaaa acggtaatgc aaaactggat gcagcgcagt cagcaaacgc
     5941 tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt gaggggatga
     6001 cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga ggagattttg aggatggttg
     6061 agagggttgc agggtagtag ataaatttta gataacaaaa aacccatcaa ccttgaaccg
     6121 aaatggcggg gttgatgggc tccacaaaat ggggacatca aagaaaagca gtggcactaa
     6181 ttaagactga tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc attttcaggg
     6241 caacttcagt tttatcctaa tcctggccat accatgacga tgattgtccc tgccagcgtc
     6301 agcaggacgt tggcgattgc gtaggtgccc gcatagccca gcgcagggat gttactgcga
     6361 gctgtatcac tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat cattgcgccg
     6421 aacaacagtg cgcggttcat tcgcaataca taagcaccga acaagaaaca gataaccacg
     6481 ggcacaagac tgacaattaa tccggcaatc aacatctgac cgccaatcgc gcccaggccg
     6541 ttattaatac cgctaccggc gctcagacca acgcctgcca taaacaccat caagccgaac
     6601 tctttcacca tgcttaatgc accttgcgga atgtaaccga aggtcgggtg gttagcacgc
     6661 ataaagccca gcataattcc ggcgaataac aacccggcag cgttccccat gccgaaactg
     6721 aatgtgctga actggaaggt gatcatcccg atcatcagcc caataacaaa gaaggcgcaa
     6781 aatgccagca ggtcagtgac ctggctgtga atcgagataa agccgatgcg atcggcgatg
     6841 gtttttacgc gacgggcatc accgctgact tgtaaaacgt cacctttgtt aagcacgacg
     6901 ttgtcatcta tcggcatctc aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga
     6961 tcggtcaact tcagttgtgc gagacgttta cctacagcgt tatggttttt aacgaccact
     7021 tcttcagtga cgatacgcat gtcgagaagg tcacgatcga aaacttcttt accgttacgg
     7081 aagctgggat cgagtcgggc atgggcgtcg ggatagccta ccaacgctat ttcatcgccc
     7141 atttgtagca cggcatcacc gtctggattt gccagaatcc cgttacgtcg aatacgttca
     7201 atgtagcagc cggtttgtcg ataaataccc agttcacgca gatttttgcc gtcggtccag
     7261 gccaccagct ccgggccgac gcgataggcg cggatcaccg gtaaataaac cttacggttg
     7321 gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg cgctggtctg taagtcctga
     7381 tgctgcaatt tcggcaagta acgcgcacca acaatcaaac tcaccagacc gattaaatag
     7441 gttaaggcat acccgaggct cagattatcc agtgccagtg agagctgcct gctttccatg
     7501 ccggaatgat gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt catagagcct
     7561 gctaacatac cggccgtcag gccaatatcc cagccaaaca gcttacctaa ccctaaggcg
     7621 atcaccagcg cactgccaac catcaccagt gctaacatta ggtaattttt cccatcgcga
     7681 aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat aaacagcata
     7741 aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt gttggcctaa taacagcgat
     7801 acgactaaaa cgccaatgga attacccagt tggatcgaac caagtcgtaa ctttccgaga
     7861 catagcccaa gcgcgaggac cacaaataat aacagaatgt aattcccatt taacaattcg
     7921 gcgacgttta tattcacgga ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg
     7981 ctaatttacg ataatgtgtt ttaccagaat tcagggcgca gattcattca gcgcacctaa
     8041 acgatagtaa agtaacaata tattttacta gtgtaatcac attaggtatc aacggctata
     8101 tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca
     8161 cactggcgaa aaatgtgttc gatagacgca gtgtcaggag gaacgagtga aacataaaca
     8221 acgttgggcg ggggcaatct gctgttttgt cctcttcatt gtggtgtgcc tttttctggc
     8281 gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct gaaatcggct tgctgttttt
     8341 cattcttcct ggagcagttg ccagtttctt ttcacagcgt agagaagtcc tgaaacctct
     8401 atttggcgca atgctggcgg caccctgttc aatgctcatt atgcggctgt ttttttcacc
     8461 gacgcgctca ttctggcaag agctggcatg gttactaagc gcggtgttct ggtgtgcgct
     8521 gggggcactg tgtttcttat ttatcagtag tttgtttaaa ccacagcaca gaaaaaatca
     8581 gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg tccagatttt ctttcaccca
     8641 tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg acaaaaatct gcggcacggt
     8701 ttctacgggt ttacctgcct tttgttgtag atcttcttta gtgatccctt ccgcacgaat
     8761 atctacatac tgatactgaa aatcatcgcg ttcattgctc aatttctcag ccagatcttt
     8821 tgcacgcaca cagtaagggc aacccgaacg accaaaaata acggtttgca ttatttctct
     8881 cctcatagat ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa
     8941 cctgttgcat taattgctaa aagctataac tgttaaacac aatacagtga aaagttttag
     9001 actgaaggct cactttgcag agggaagcgt atgcgcgcga tcggtaaatt gcctaaaggc
     9061 gtgttgatac tggaatttat cggaatgatg ctactggcgg tggcgctgct gtcggtaagc
     9121 gactccctgt cgctgcctga gccattttct cggccagaag tgcagattct gatgattttt
     9181 ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta ttcttcaggt ggcaaaacgt
     9241 cttgccccac agctgatgaa ctgtccaccg caatattcac gttcagaaag agaaaaagat
     9301 aatgacgcca accattgaac ttatttgtgg ccatcgcgcc attcgccatt tcactgatga
     9361 acccatttcc gaagcgcagt gtgaggcgat tattaacagc gcccgtgcga cgtccagttc
     9421 cagttttttg cagtgtagta gcattattcg cattaccgac aaagcattac gtgaagaact
     9481 ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg gcggagttct gggtgttctg
     9541 tgccgacttt aaccgccatt tacagatctg tccggatgct cagctcggcc tggcggaaca
     9601 actgttgctc ggtgtcgttg atacggcaat gatggcgcag aatgcattaa ccgcagcgga
     9661 atcgctggga ttgggcgggg tatatatcgg cggcctgcgc aataatattg aagcggtgac
     9721 gaaactgctg aaattaccgc agcatgttct gccgctgttt gggctgtgcc ttggctggcc
     9781 tgcggataat ccggatctca agccgcgttt accggcctcc attttggtgc atgaaaacag
     9841 ctatcaaccg ctggataaag acgcactggc gcagtatgac gagcaactgg cggaatatta
     9901 cctcacccgt ggcagcaata atcgctggga tacctggagc gatcatatcc gccgaacaat
     9961 cattaaagaa agccgcccat ttattctgga ttatttgcac aaacagggtt gggcgacgcg
    10021 ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga ccaggtctga cagagaggtg
    10081 cagggtgaaa attgccatat tgtcccggga tggaacgctc tattcgtgta agcggctgcg
    10141 tgaagccgct atacagcgcg gtcacctggt tgaaattctt gatccgcttt cttgctacat
    10201 gaacataaat cctgcggcgt cttctattta ctacaaaggc cgcaagttac cccattttga
    10261 cgcagtgatc ccgcgtattg gcaccgccat taccttttat gggacggcgg cactgcgcca
    10321 gttcgagatg ctggggagct atccgctcaa tgagtcggtc gccattgccc gggcgcgtga
    10381 caaattgcgt tccatgcaac tgctggcgcg tcagggcatc gacctgcctg tcacgggcat
    10441 tgcgcattcg ccggatgata ccagcgattt aatcgacatg gtcggtggtg cgccgctggt
    10501 ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg ctggcggaga ctcgtcaggc
    10561 ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg catattctgg tgcaggaata
    10621 tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt gttggtgatg aagtggtcgc
    10681 tgcgattgaa cggcgggcga aagagggcta ttttcgttcc aatttgcatc gtggcggcgc
    10741 ggcaagcgtc gccagtatca caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac
    10801 gatggcgctg gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat
    10861 ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa accaccggta tcgatatcgc
    10921 gggtaaaatg atccgctgga tcgaacgcca cgctacgaca gaatattgcc tgaaaacggg
    10981 tggttagtcg caatcacatt actgatcatg gttttgcctg cgctttttgc gtaagctgtg
    11041 ccggtctttt tatcgaaaga ggttgtacaa aattatgaca tcgctggtcg ttcctggtct
    11101 ggatacgctg cgtcaatggc tcgatgacct ggggatgagt ttttttgaat gtgataactg
    11161 tcaggctctg catctgcccc atatgcagaa tttcgacggt gtctttgatg ccaaaatcga
    11221 tctgatcgat aacacgatcc tgttttctgc catggcggaa gtccgacctt cagccgtatt
    11281 gccgctggcg gcggatttat ctgccatcaa tgccagttcg ctgaccgtga aagcatttct
    11341 tgatatgcag gatgataatc tgccaaagct ggtggtttgc cagtctttat ccgttatgca
    11401 gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat
    11461 ggtcattctt gaagctaatg cccatcaact gctgttaccg actgacgatg aagggcaaaa
    11521 caacgttacc gaaaactatt ttctccactg ataattcctt tcgagcacgc agtcgctgcc
    11581 gcagtgactg cgcgctgcaa aattatctgc tgtttttaac cttttcttaa agattatttc
    11641 acttctcttg tgtcgatttg gctttatcac atagagcaaa tacgcataaa aatttgttaa
    11701 ataccgtttt ttaatccgag ctatagtctc aaaccctggc taaagttatt cttgcgatgc
    11761 ttctatatag cgagcagtgc tggccgggag agagttctct tttcttacac cgcgccgata
    11821 aaaaaatatg cacgtttatt gcatatcttt cagtgtgaca acttttgttc gtttgttaac
    11881 gaactttcag aaggaaagag atgtgaccgc cttaaataaa aaatggctat cgggtctggt
    11941 tgcgggtgct ctgatggccg tctctgtcgg cacgctcgcg gctgaacaaa aaacactcca
    12001 catttataac tggtctgatt atatcgcccc tgacacggtg gccaattttg aaaaagaaac
    12061 cggtattaaa gtcgtctacg atgttttcga ctctaacgaa gtactggaag gcaaattaat
    12121 ggcgggtagc accggctttg acttggtggt tccatctgcc agctttctag aacgccagtt
    12181 gactgcggga gttttccagc cgctggacaa aagcaaattg ccggagtgga agaatctcga
    12241 cccggagctg ctgaagctgg tcgccaaaca cgatcccgac aataaatttg ctatgcccta
    12301 tatgtgggcg acgaccggga ttggctataa cgttgataaa gttaaagctg tgctgggcga
    12361 aaacgcgccc gtcgatagct gggacttgat cctcaaacct gaaaatctgg aaaaactgaa
    12421 aagctgcggt gtctctttcc tggatgcgcc agaagaagtt tttgctaccg tgttgaatta
    12481 tctcggcaaa gatcccaaca gcactaaagc ggatgattac accggaccgg caaccgatct
    12541 gctgttaaag ctgcgcccga acattcgtta tttccattca tctcaataca ttaacgacct
    12601 ggcaaacggc gatatttgcg tcgctatcgg ctgggcaggt gatgtctggc aggcgtcaaa
    12661 ccgcgcgaag gaagcgaaga atggagtgaa tgtctcgttc tcgattccaa aagaaggggc
    12721 gatggcgttc tttgatgtat tcgccatgcc agcggatgcc aaaaacaaag acgaagccta
    12781 tcagttcctg aattacctgc tgcgcccgga tgtagtagcg catatttccg accatgtgtt
    12841 ctatgtcaac gccaataaag cagccacgcc gctggtgagt gcggaagtcc gtgagaaccc
    12901 gggtatttat ccgcctgcgg atgttcgtgc gaagctgttc actctgaaag tgcaggaccc
    12961 gaaaatcgac cgtgtgcgca cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc
    13021 agccttagat gccgggcggg cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc
    13081 ttgcacacat tcaatgccgg agagcagccg tgaatgacgc tatccctcgc ccgcaggcga
    13141 aaacccgtaa ggcgctgacg ccgctattag aaatccgcaa cctcaccaaa tcctacgatg
    13201 gtcaacatgc ggtggatgat gtcagcctga ccatctacaa aggtgaaatc ttcgcgctgc
    13261 tgggcgcatc cggctgtggc aagtccacgc tgctgcgtat gctggcaggt ttcgaacaac
    13321 cttctgccgg acagataatg cttgatggcg tcgatttgtc acaggttccg ccttacctgc
    13381 gccccatcaa tatgatgttt cagtcttacg cgctgtttcc gcatatgacc gtggaacaga
    13441 acatcgcttt tggcctgaaa caggacaaac taccgaaagc ggaaatttcc agtcgggtca
    13501 atgagatgct cggcctggtg cacatgcagg agttcgccaa acgtaaaccg catcagcttt
    13561 ctggtggtca gcgacagcgt gtggccctgg cccgaagcct tgccaagcgc ccgaaactat
    13621 tactgctcga cgagccgatg ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg
    13681 aagtggtgga tattctggag cgcgtcggtg tgacttgtgt gatggtcacc cacgatcagg
    13741 aagaggcgat gaccatggcg gggcgcatcg ccattatgaa tcgtgggaaa tttgtccaga
    13801 ttggcgaacc ggaagagatc tacgagcatc cgactacccg ctatagcgcc gaatttattg
    13861 gctcggtaaa cgtctttgaa ggcgtactca aagagcgtca ggaagatggc ttggtgcttg
    13921 attcgccggg gctggtgcat ccactgaaag tcgacgcgga tgcctcggtg gtcgataacg
    13981 tgccggtaca tgtggcgctg cgcccggaaa aaatcatgct ttgcgaagag ccgcccgcca
    14041 atggttgtaa cttcgcggtg ggggaggtga tacacattgc ctatctcggc gatctttcgg
    14101 tgtatcacgt tcgtctgaaa agtgggcaga tgattagtgc ccagctacaa aacgcccatc
    14161 gtcatcgtaa agggttaccg acctggggcg acgaagtgcg tttgtgctgg gaagtggaca
    14221 gctgtgtggt gctgacggtt taaggagcaa agatgaatac acttgaacct gctgcccagt
    14281 cgaaaccgcc gggcggattt aagctgtggt tgtcgcagct gcaaatgaag catgggcgca
    14341 aactggtcat tgcgttgcca tatatctggt tgatcttgct gtttctgctg ccatttctga
    14401 ttgtctttaa aataagcctg gcggagatgg cgcgcgctat tccaccttat accgaactga
    14461 tggagtgggc tgacgggcaa ctgtccatca ctcttaatct cggtaatttt ctgcaactga
    14521 ccgacgatcc gctctatttc gatgcttatc tccagtcgct acaggtggct gcgatttcga
    14581 cattttgctg tttactgatc ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt
    14641 cgacccgtaa tattttatta ctactggtga tcctgccgtc gtggacctcg tttctgatcc
    14701 gcgtttatgc ttggatggga atattaaaaa acaacggtgt gctgaataat tttctgctgt
    14761 ggctgggggt tatcgatcaa ccgctgacca ttcttcatac caatctggca gtttatattg
    14821 gcattgttta cgcctacgtg ccgtttatgg tgctgccgat ttataccgcg ttgattcgta
    14881 ttgattattc gctggtggaa gcagcgctgg atctcggtgc acgaccgctg aaaacgttct
    14941 ttactgtgat cgtgccgctg actaaaggtg ggattattgc cggatcgatg ctggtgttta
    15001 tcccggctgt gggcgagttt gtgatcccgg aactgctcgg tggcccggac agcatcatga
    15061 tcgggcgcgt gctatggcaa gagtttttca ataaccgcga ctggccggtg gcctcggcgg
    15121 tagcgatcat catgttgctg ctgctaattg tgccgataat gtggttccac aaacaccagc
    15181 aaaaaagcgt gggagaacac ggatgaataa tttaccggta gttcgttcgc cctggcggat
    15241 tgtgattttg ctgctgggct ttacctttct ctacgcgcca atgctgatgc tggttatcta
    15301 ttcgtttaac agctcgaagc tggtgacggt gtggggcggc tggtcaacgc gctggtatgg
    15361 tgagttattg cgcgatgacg cgatgatgag tgcggttggt tcaagcctga caattgctgc
    15421 ctgtgcggca acggcggcgg cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt
    15481 tggcaggttt cgcggatcaa atggctttgc ctttatgatc accgcgccgc tagtaatgcc
    15541 agatgtcatc acgggcttgt cgctgttgtt attattcgtc gcgcttgctc atgccattgg
    15601 ctggcctgcg gaccgcggta tgcttaccat ctggctggcg catgtcactt tctgtacggc
    15661 ttatgtggcg gtcgttattt cgtcgcgtct gcgggaactg gatcgctcga tagaagaagc
    15721 cgcgatggat ctcggtgcga cgccgctgaa agtgtttttc gtcattacgc taccgatgat
    15781 catgcccgcg atcatttctg gctggttact ggcttttact ttgtcgcttg atgatctggt
    15841 gatcgccagc tttgtttccg ggccgggagc caccacgtta ccgatgctgg tcttttccag
    15901 cgtgcggatg ggggtgaatc cggaaatcaa cgccctggca acattgatcc tcggcgcggt
    15961 cggaattgtc ggatttatcg cctggtatct gatggcgcgc gcagaaaaac agcggatacg
    16021 cgacatccaa cgtgcaagac gtggctgaag acactaaaat ttgccaacct ggctacataa
    16081 tgccgcgcat gtcgcggcat tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa
    16141 acatcttcat ctcatgctcg cctgaatgtg cctgcgctgg tgcaggtggc ggcgctcgcc
    16201 attattatga tccgtggcct cgacgtgctg atgattttca atacgctggg cgtgcgcggt
    16261 attggcgagt tcattcatcg cagcgtacaa acctggagtt taacgctggt ctttttaagc
    16321 agtctggtgc tggttttcat tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc
    16381 tgggcgcgct ggctatatct gctgacacaa atcaccgccg caagttactt gtgggcggct
    16441 tcgctggggt acggttatcc ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc
    16501 ttccatagcc tgatgctgca gaagctgccg gatatgctca tcctgatgct gctgttcgtt
    16561 ccctcgacca gtcggcggtt cttccagttg caataatgtg tataatcgtc gcccctgatg
    16621 atgtgaaggt caatgtatgc agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca
    16681 gtggataacg cagccgattc cagagcaact ctccgctaaa accgccgatc ttaaaaatct
    16741 gctcgccgat tttccggttg aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt
    16801 tcgtaataaa gccaaaatgg tggtgagtgg tagcgttgaa aaaacactgc tcggtatgct
    16861 gcatcgcgat ggcacgccgg aagacctttg tgactgcccg ctttatccag cctcatttgc
    16921 gcccgttttt gcggcgctaa aaccgtttat cgcccgagcg gggttaacgc cctacaacgt
    16981 ggcgcgtaag cgtggcgaac tgaaatacat tctgctgact gaaagccaga gtgatggcgg
    17041 catgatgctg cgctttgtac tgcgttctga taccaagctg gcgcaactgc gtaaggcgct
    17101 gccgtggtta cacgaacaac taccgcagct gaaagttatt accgtcaata ttcagccggt
    17161 acatatggcg attatggaag gggagacgga gatctacctg accgaacaac aggcactggc
    17221 ggagcgtttt aatgacgtac cgctgtggat ccgtccgcaa agtttcttcc agactaatcc
    17281 ggcggtcgcc agccagttgt acgccaccgc gcgcgactgg gtacgacagc tgccggttaa
    17341 acatatgtgg gatctgttct gcggtgtggg gggctttggt ttacactgcg cgacgcctga
    17401 catgcagtta accgggatcg aaattgcgcc agaggccatt gcctgtgcaa agcagtcagc
    17461 cgctgaactg ggcttaactc gtttacaatt tcaggcgctg gactccactc agtttgccac
    17521 tgctcagggg gaggtgccgg agctggtgct ggttaacccg ccgcgccgcg gcattggtaa
    17581 accgctgtgt gattatcttt caacgatggc accgcgtttt attatctact ccagctgtaa
    17641 cgcccaaacc atggcgaaag atatccgcga actgccaggt taccgcatcg aacgggtaca
    17701 gctttttgat atgttcccgc acaccgcgca ctatgaagtg ttgacgctgc tggtgaagca
    17761 ataaaaaagc cacaggtgcg gcttcagatt gctgatcagg tgcgctttgt tcatgccgga
    17821 tgcggcgtga acgccttatc cggcctacaa aatcatgcta aatcaataca ttgcagagtt
    17881 gatgtgggcc ggataagcgc agcgcatcag gcaatcttac ttttgtcatc agtctcaatt
    17941 actgcggaaa ccactggtca ttgattttct gatatgtacc gtcagcttta attgctgcca
    18001 gcgcgttatt cagtttttcc agcagagctt tgttatccgg acgtacagcg atacccaggc
    18061 cggtgccgaa gtattgcgga tcggtcactt tttcagtcgc aacgcccagt tgcggattgg
    18121 ttttcagcca ttcgtttacc accgcggtgt caccaaatac cccatcaata cgaccatttt
    18181 tcagatcgat aaaggcattc tgataactgt cataggagac agttttcact tccgggtgct
    18241 gatcctgaat atatttctgg tgcgtcgtac cgttttccat cccaatgcgt ttgcctttca
    18301 ggtcggcaaa cgttttgtag gtatcttttt tggaaatcac gacggctgag ttttcatagt
    18361 agggtgtggt aaacgaaacc tgcttgctac gctccggggt gatatccata ccggagatta
    18421 cggcgtcata ttttctgaat ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa
    18481 aagtacattc tgcctgcatt tgtttgcaca aggcttttgc cagatcgata tcaaagccga
    18541 caatctcatt attagcacct atagattcaa agggtggata ggtggctgaa acgccaaaat
    18601 tgattttctc tgcggcagaa gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta
    18661 actttttcat gatggaactc ccgtctgtca atcttatgat ttttggccgt gtctgcggca
    18721 tgggataaca atgccatcaa gtgaatttat atgcaataaa catgattaaa taatttaaat
    18781 gaaataaaaa aagacggata acttagtgag ttgtccgtct tcattataag aatttatgca
    18841 ctatgtaggc cggataaggc gtccacgccg catccggcac aggcaccgtg ctgatgtctg
    18901 atgcgacgct ggcgcgtctt atcagaccta caaacccccc cggcgaatgt acgcagctac
    18961 attaattccg ccgttcgaat gccagtgctt tgcgctcgat cagacgcatc atcagcgtca
    19021 gcaggccgtt gacgaccaag taaataatcc ctgccgcacc gaacaccatt acatcgtagg
    19081 tgcgtccgta caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca
    19141 gagaggtact tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa
    19201 aggcatacgg cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg
    19261 actgccactg accttctggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg
    19321 ccgcactgtt cagcgataac gcaatcagcg cacataacca cggttctgac aacaaatgcc
    19381 acagtgccgg atactcctgc aacgtcggaa actggcccgg cccgtaataa atcaggaaga
    19441 tctgcaccag cagcggcgta ccggtaaaca gcgtgatata accccgcacc agccacacca
    19501 gcaccggcgt tttcagcgtc aggatgatgg taaaaatcaa tgccagaatc agtgccacaa
    19561 tcagcgaggc aacggttagc gtcaggctgg tgtgcagccc tttcatcagc tcgggtaaat
    19621 actcaaacat tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag
    19681 aatgtactga ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt
    19741 aaatggttcc tgggtacgag tagcaatgct tttggtttgt aacattaaat cattcacact
    19801 aatcaaactg accagcgcgg tatctttcag cagcaccagc cactggttac cgaggccagg
    19861 cagcgcatga cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag
    19921 ccccagcgcc tgaccagatt cccactggcc caccggtaca gctttcaacg cgccgcgcag
    19981 cgtttgcgag gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg
    20041 actcacgtcg aagttctcaa tgtccatctg caccgggatc tgcacgaacc caagattgat
    20101 agtgaagcca tccgaaagcg tcagcagtaa ctgcgaggag ccaaaataga taaacagcac
    20161 taccagaatt tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac
    20221 aggacgccat ttcgctgact cccataccgc aaagaacatc gccagcgcca gcccgacaat
    20281 caatgcacaa acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc
    20341 attcatcagg aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt
    20401 cttcactttt tccagcgcag tgttgagttt ctgctgcagt tcagtgtttc cctgacgtac
    20461 cgcgatgccg aggccagtac cgaaataatc tttatcggtc actttatcgc ccactgccgc
    20521 cagttttggg ttatctttca gccactcagt gaccactgcg gtgtcaccaa atacgctgtc
    20581 gatacgcccg ttttgcagat ccagttttgc gttctggtag ctgtcatacg gaacggtgat
    20641 gatttccggg tgcttatcca taatgaactt ctggtgtgtc gtcccgttct gaacgccgac
    20701 ttttttgcct ttcagctgat caacactggt gtatttgcct tgctgaccca caaacagggc
    20761 agagttgtca tagtacgggg tagtaaacag tacctgcttt tcacgctccg gagtgatatc
    20821 catgcccgcc atcactgctt ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa
    20881 cgcctggtta gagaaagtac aggttgcatc aatctcttta cacagtgcct gcgccaggtc
    20941 gacgtcaaaa ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gatagggggc
    21001 ttcggtagca aaacgaatgg tttcggcagc tgtggcggaa agactaaaac ctgcaattaa
    21061 cgcggcaatc agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt
    21121 ttaaatgctt cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga
    21181 ccattttcca tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg
    21241 acgatcacct gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc
    21301 gtaatttccg ggtccagtgc ggcggtcggt tcatcgaaca gcagtacctg cggttccatc
    21361 atcaacgcac gggcaatagc aacacgctgc tgctgaccac cagaaagatg cagcgggtaa
    21421 cgatcgctat aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc
    21481 tgatctttac tcaaccccag tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc
    21541 agatgcggcc acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga
    21601 atcgctttgt cagagggtgt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta
    21661 ccggagcgcg gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg
    21721 ctggggccaa gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc
    21781 gcctgatgcg cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt
    21841 atactggcag tctgatagct attgaggtcg aagatagtac ctttaacaga ataattatgc
    21901 aatatttatg ctttaaaagt taaaagcaaa gcgcattatt caataaacat agcacaaaat
    21961 aacgggggcg gtggtcggtg agcataaatg tcggcattcc tcacgaaatg ccggataatt
    22021 tacggggttt attggttgat caaggcgtta gcgattctcg atggactgac ggagcgtacc
    22081 cgccgtggca tgaacgctac cgcctaagta gcgcacatcg tcgatgaccc aacactggcc
    22141 ttcctgaatc attaacactt catcctgcca gccctggtca ccctgtttga gatccacccg
    22201 caatggaatg ttacgggcat cacgattagg gatagtcgat gcactggcaa cgtgggcgct
    22261 gtctggcaag gtggttcgac tggagaacgg atcgctgctt aacaattcac gatgactatt
    22321 atcgcggctg gcatcgctaa gttgggttgc cagtttgtca ctcaaatagg ggcgcagggc
    22381 ggtaatgtca ttgctgcgat gttgaatgcg gtagtcgtag aattgttgag ccacactgtc
    22441 aggccctccc tggacacagg aaccggtgcg ggggccgtta tctttataag ctggcgtgac
    22501 ggtggtacag gcactcagca gcagtgcaca tggaataagt agagatagtg aacggtagcg
    22561 cataatcatt tccttattag cttgctgtct gcgtcacact acgaccaaac gctatcagat
    22621 tatttaatac attgttttta atacaattct gaaacaatct gaacacctga ttgggtttga
    22681 gtccattgct ggcgacccac tcaaggttgc tcgtagctga cttacttaaa ttacccaaac
    22741 aatagacagt gtaaaccatt tgtgaggttg agcgtgttat atggctgaaa tgatcattta
    22801 aggtaaatat gatagttctg aaatggtggt ataaatcctc ctgtagaggt taagagcatc
    22861 tgcaagaagc cagtttcaca tttataacgc gtgataaatg aatgcggtta ttgtctaatc
    22921 attacaaaat ttttcacatg cgataagaga tcctgcgatt aagatgattg ccaaaattac
    22981 gttgtatttt tgaaaatgag cgaaatttga tagtcccagc tttgactctc gataatatta
    23041 tttatgctac aattgtgggt aaagaattta agctaatgaa tttgagtgaa gaataaatta
    23101 aattgcttca gtatttcgtt aattaatata aatacaaaag gcgttgtgct tttataatta
    23161 taaagcgcga ggagttcggt ggattatcgt ttttcttggg tgatttttaa tatgataaac
    23221 aatatatctg atcaagcatc atcatttcca ggcacacaac taaatcaaag tgataatttc
    23281 ctggattcac tgagggaatt tttcgcgata ttaaatccca gtcgtaaggg tgagttgtct
    23341 acatgggata ccatctactt gcatctgata ttagcaataa atgctgacag tgatctcata
    23401 aaaaatgatg tgttgctcgc ggaaaatatt ccatcagcga attaccaatt taatacattt
    23461 tttagcaata ctttcgagat tgatgttaaa aaatatctgg gtaaatcaga agataacgaa
    23521 gttgaaataa aagcgggtaa tgaaagaatc tctattggaa tacgtaatgt atcgaatgga
    23581 aaacttgaaa gacaacagtt tcttttccca ctggactatg agaataaatt acaagaacaa
    23641 ttagataagt attttacgat tgaatcgcac ccattattat acaggtatac cattggtagt
    23701 aagattgcga atgtgatttt tgaaaagtta tacagtcgta ttgactttaa caaagaacaa
    23761 tatatatcct ttataaagga cgcatttatt catttttatg attatagcag gcgatatgct
    23821 attagtgaga atatcgataa agacgctgta acaaacaata tcgcattgat gagcactttt
    23881 tatgacagtg ataatacgtc aggtgaggtt ttaaataatg attttacaga agaagagtca
    23941 tttgaaactg cgttagatgt agagcatgca atagtgcttg gatttgctga tgataatttt
    24001 gaaacaaaac ctgttcatta tcaggattta ttaacacgat ttagtgcctt tcaagatacc
    24061 gtgtttaatc tgtttcctga aatgcactct tcgcattacc acgatatttg tagcgtaagt
    24121 gtggatatga caaagggaac gcaatgcatg attcacttga tggtgaatga agaagttttc
    24181 atgtctctgc ctgttccagt tgccacgatg gtacgtgaag atgcaagtaa tttagtcaat
    24241 cttaaaacac tactcaatga tggttgtttc attaaatata gtcattttaa tgacgtggca
    24301 ttaataaagc aaaacataag taatctctat ttatcacaca ctgttgtgaa tgaaagcata
    24361 ttgaaaaaat gttgttttga aaacggcagt ttaggtgatg ttaaaataac aaatagcaat
    24421 gtaataaaca gtgcattcaa aaatatttcg tttagatctg ttaaaattaa taacgtaaat
    24481 actcattctt taaaatttat taactgcaat tttttcaatg ttgatatgat aagagttaat
    24541 ctcagcaaat gtctttttca tgaatgtagt atgcatggtg tcaagataaa accctggttg
    24601 cctgtaaaat ggacaaaaga attaataagt gattatttgt atggctgttt actatctctg
    24661 tactctatct gtgcaaggga tatatataat atgaacgccg ggaataacgt gaaggttgct
    24721 gcggatgctt tccttgaaat tattttctct cttaaaaata aatattgtat taaactatta
    24781 agcgctcagg atcgggcgtt tatatacgag tttgcaagaa tgatcttcgc ttatattaat
    24841 gacaaaagca ttgagatctt attactttct tgttttgcgg cggctgacca aaaagcaatc
    24901 cagcggtata gacctcagtc acaagatggg gaagacttta ggagtcattt acaatataag
    24961 ttgcctcttt ccgctcatta atgttgttgg tgcaacataa taagacaggt aagcggtacc
    25021 ctataatgaa accaatatcg ggttatgcaa cggataatcg taaagactta agtgggtgtt
    25081 tttgacacaa tgagatgatt tcctaaactg atcaaaacct tcttcccaac atggcatatc
    25141 agtccgataa tgctttagag atcgaaggct taacaaacaa ggagatcgat catgcaattt
    25201 tctacaaccc caactctgga aggcctgacc atcgttgaat attgcggtgt ggtgaccggc
    25261 gaagcgattt taggtgccaa tattttccgt gatttctttg ccggtatccg cgatatcgtt
    25321 ggcggacgtt ccggtgcgta tgaaaaagaa ctgcgtaaag cacgggagat cgcctttgag
    25381 gaattaggct cccaggcgcg ggcgctgggg gccgatgccg tagttggtat tgatatcgac
    25441 tacgaaacgg tcgggcaaaa cggcagtatg ctgatggtta gcgtcagcgg tacggcggtg
    25501 aaaacgcgtc gatgagaaga gtcttctggc tggtcgctgc cgctctgtta ctggcagggt
    25561 gtgcaggcga aaaaggcatt gtcgagaaag agggatatca gcttgatacc cgacaccagg
    25621 cgcaggcggc gtatccgcgc attaaagtgc tggtgatcca ctacaccgca gatgattttg
    25681 atagctcgct ggcgacactg actgataagc aggtcagctc gcattatctg atccctgcgg
    25741 tgccaccgcg atacaacggt aaaccgcgca tctggcaact ggtgccggaa caagaactgg
    25801 cctggcatgc ggggattagc gcctggcgcg gggcaacgcg ccttaacgac acctctattg
    25861 gcattgagct ggaaaaccgt ggctggcaaa aatcatccgg agtgaaatat tttgccccgt
    25921 ttgaacaggc acagattcag gcgcttattc cgctggcgaa agatattatt gcccgttatc
    25981 acatcaagcc ggaaaacgta gtggcacatg cggatatcgc accgcagcgc aaagacgatc
    26041 cggggccatt atttccctgg cagcaactgg cgcagcaggg gattggtgcc tggccggatg
    26101 cgcagcgggt gaacttttac cttgccgggc gcgcgccgca cacaccggta gataccgcgt
    26161 cattgctgga gcttttggcg cgctacggtt atgacgtaaa acctgatatg acaccgcgcg
    26221 agcagcggcg cgtgattatg gcattccaga tgcatttccg cccgacgtta tataacggcg
    26281 aagcggatgc agaaactcag gcgattgccg aagcattgct ggagaaatac gggcaggatt
    26341 agcgcggcag ttttccgtgg tcgcgtagcc aggcggcagt tttctcgatg ccttcatcca
    26401 gggtgatgac cggctgataa cctaactctt cctgcgcacg cgtaatatcc agcgtaaagt
    26461 caaaattcaa cttggaggcg ccgtagtggg tcagcggcgg ctcttttgct gacttgcggc
    26521 ctaaacgctc catgctgcgg gcgatcatat ccagcatcgg gtaagggacg gaacgaatac
    26581 gacagtcaat attcaactcg tcgatcagct tctgcacgat gctgcgcagt gtgcgatgct
    26641 cgccgttggt gatgttgtac acacgcccgg aaggtagctt atcgcaggct tcctggcttg
    26701 ccagccacat tgcgtgcacg gcattttcat agtaggtcat atcgaccagc gcactgccgc
    26761 catgcggtaa cagaatactg ccgtagtggt gcatcatatg cgccagacgg ggaataaaga
    26821 ctttatcgtg cggtccgaac agactttgtg ggcgcagaat agtaaagcgc gtttgtggat
    26881 tcgcctgcga aagcatattg atcacttctt cgctggctgc tttgctgcgg gcaaactcgt
    26941 tggcgaagcg gtgagggcga aaatcttctt taatattgcg atggtggtga taatcgaagt
    27001 acagggaggg ggaagagata tgaataaagt tacgcacacc ccaggcgaca gcccattcac
    27061 ccaggcggcg agtggcgcga acgttagcca gatcgaaagc ctgttgtgtc ccccagggtg
    27121 aggtaaagct ggagcagtgc cacagcgtat caatgcccgc gagcatcact ttagcttgtg
    27181 atgaaaccag ctcggtcaga tccgtcggaa caaactctgc gcccattttt tccagcaatt
    27241 tacccattgc ttcgttgcga ccgctcgcac gcacgctgat gcctttctgg cataaaaact
    27301 ctaccgcgtt tcgacctaag ccgctggtgg cgccggtaac cagtaccttc atatcaatcc
    27361 actgttgttg agaaaatgac gtgcgcattc ttccgtgatt tcccccatga tgcaatggga
    27421 aacatgaaag aataacgcag gttttgtcga ttaatctgtg ctttgttctg ccagtctggc
    27481 gatttgtttt gccattccgc gaaaaataaa cagatgcgcg gggatcatca ataaccagta
    27541 aaacagcccc ggcataccgt gcggatgcca gaaagcgcgg acatcgatag tacgatagtc
    27601 acctttatct tccagggtaa aacacagtcg tcccagcccc ggcgctttca tgccaaataa
    27661 caacgtaagt tgctttttcg gttcaacgac aatcactttc cagctatcca ccgcatcgcc
    27721 ggtctgtaaa tattcgcgct ccgggcggcc tttcgccaga ttatgaccga tcgcgcggtc
    27781 catcaacgcc cgtgtctgcc acaaaatatt gccaaagaaa taacgctctt taccgccgat
    27841 ttggttcacc accagccata aagccgcaag gctggcggac gttttaacgg taaaccccgc
    27901 ctgtttggcg aaataaccgt actccggtcg ccagcgggca aaggcctgag cgtcgtagcc
    27961 ccagtcgctg gagttgacca gtttttcttc ctctttcaac gtgctacgta ccgcgtcatc
    28021 gaaagcgatc agccgttgtg ggatgagtgc acgtagcgcg gtatcatctg ccagcagatc
    28081 gtgtttcagc ccctgaatca acgccctggc ggtggtgggc ggtacggaag taatcacatt
    28141 gagaaaccac accgaaatcc agcgggtggg gagggggatg gggatcaacc agcggcgctt
    28201 accgctcacc gccataaaat gttcaaactg ttgctgataa ctgagcacct ctggtccggc
    28261 ggcttcgaag atgcggtgtt cgttggctgg atggtctaac agcgccacca gatagtgcag
    28321 caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc cagcgtggcg gcgttaacac
    28381 tggcaggttg tagaccatat cgcgcatgac ttcgaacgcc gctgaacctg cgccaacgat
    28441 aattccggcc cgaagttcgg tcactggtac attcgcttca cgaagaatgt ccgccgtagc
    28501 ctgacgagct cgcagatgat ccgactgctc atgtggcggg gcctgcaacg aactgagaaa
    28561 gattaattgc ttaactggta cttcacgtag cgcatcgcgg acgttgagag ccagctggcg
    28621 ctcctgagcg ataaaatcgc cgccttcgcc catgctgtgc accagaaaat agacggtatc
    28681 gatatcctgc aacagggccg gaaggttatc cggccagctg agatcgactt tatggcaact
    28741 gacgtttgcc agttgcagct ttgcaagcct gtcgacatga cgtgccgccg ctaggatctg
    28801 atgcccttgc tggctgagtg tgcgcaccag atgctgacca atgtagccac tggcaccgag
    28861 aactaaaatg cgttgcggca cgtctctctc cttaacgcgc caggaatgcg cgccagtggg
    28921 cggcgacttc cgccagttgt tcgcgcgaga cgtcaagatg cgtcaccagg cggacaatcg
    28981 gcgaggcgtt aatcagcacg tttctcgctt tcatgtattc gcctaacgcg gcagcatttt
    29041 cttccccgac gcgaacaaac agcatattag tgtcctgacg catcacatcc gcgcctgctt
    29101 cacgcagttg ctccgccatc caggcggcgt tgtcgtgatc ttcctgcaac cgtgcgacgt
    29161 tattttccag ggcatacatc ccggcggcag ccagaatccc ggactgacgc atcccgccac
    29221 cggccatttt ccgccagcgg atggcacgtt taatgtaatc acgattaccg acgagtaatg
    29281 aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt gaacgaatca caatattgcg
    29341 tgatctcttt cagttcgcag ccgtaagcca ccacggcatt aaagatgcgc gcaccgtcaa
    29401 catgcagcgc cagattgcgc tcgcgggtaa attcccatgc ttctttcagg tattcacgcg
    29461 gcagcacttt gccgttgtgg gtgttttcca gactgagtaa tttggtgcgg gcgaaatgga
    29521 tatcgtcggg tttgattttc atcgccactt tatccagcgg tagcgtgccg tcggcagccg
    29581 cgtcgatggg ttgcggctga atactgccca gcaccgctgc gccaccggct tcaaacagat
    29641 agttatgcgc ggcctgaccg acaatatact cttcgccacg ttcgcagtga ctgagcagag
    29701 cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc ggcttcttta ccggaaagct
    29761 ctgctgcata gtcctgcaga gcattaacgg tagggtcgtc tccgtaaacg tcgtccccaa
    29821 ccggggcggc catcatcgct tcgagcatgg cgcggctcgg tcgggtaacg gtatcactgc
    29881 gtaaatcaat catggcatgt ccttattatg acgggaaatg ccaccctttt taccttagcc
    29941 agttcgtttt cgccagttcg atcacttcat cgccgcgtcc gctgataatt gcgcgcaaca
    30001 tatacaggct aaaacctttg gcctgttcga gtttgatctg cggtggaatg gctaattctt
    30061 ctttggcgac caccacatcc accaacaccg gaccgtcgat agagaaggcg cgttgcaggg
    30121 cttcatcaac ttcagaggct ttttctacac ggatacccgt aatgccgcac gcttcggcaa
    30181 tgcgggcaaa gttggtgtcg tgcagctcag taccgtctgt caggtaacct ccggccttca
    30241 tctccatcgc cacaaagccc agcacgctgt tattaaagac gataattttc accggcagtt
    30301 tcatctgcac tactgagagg aaatcgccca tcaacatgct aaaaccgcca tcgccgcaca
    30361 tggcgaccac ctgacgttcc ggctcggttg cctgcgcacc caacgcctgc ggcatggcgt
    30421 tagccatcga accgtggtta aacgaaccta acaggcgacg cttgccgttc attttcagat
    30481 aacgtgccgc ccataccgtt ggcgtgccga cgtcacaggt aaagatggcg tcatcggcgg
    30541 caaaatgact aatttgctgc gccagatatt gcgggtgaat ggctttctcg ctcggtttag
    30601 ctaaatcgtc cagccctttg cgggcatcgc ggtaatcttc cagcgcttta tccagaaact
    30661 tgcgatcggt tttttcttcc accagtggca gcaatgcgcg cagggttgat ttgatatcgc
    30721 cgaccagcgc catatctacc ttgctatgcg cgccgatgct ggctggatta atatcaatct
    30781 gaataatttt ggcatcggtc gggtagaacg cacggtaggg gaactgagta ccgagtagca
    30841 ctaacgtgtc ggcgttcatc atggtatgga aacctgacga gaatccgatt aacccggtca
    30901 ttccaacatc atacggatta tcgtattcga catgctcttt accgcgtagg gcatgaacaa
    30961 taggtgcttt aattttcccg gcaaactcaa ctaactcttt atgcgccccc gcgcagccgc
    31021 tgccacacat cagggcgata ttgctggaat aacgcagcag ttgcgccagt ttgcgtaact
    31081 cttcttcttc cggcatcacg actggctgtg gcgcatgata ccagtgcgtg gttgcccctt
    31141 ctggcgcagg ttttaacgcc acgtcgcccg gtaatacaac aacggaaacg ccacggttaa
    31201 gcaccgcttt gcgcatagca attgccagca cttgtgggat ctgctccggg ctggaaacca
    31261 gctcgcaata gtgactacat tcgcggaata gctcttgtgg gtgggtttcc tggaaatagc
    31321 cgctgccaat ttcgctggag ggaatatgag cggcaatcgc cagtaccgga acgttattgc
    31381 ggtggcaatc gaataggccg ttgatcaagt gcaggttgcc tgggccgcac gatccggcac
    31441 agaccgccag ttctcctgaa agttgtgctt cagcgccagc ggcaaacgcc gccacttctt
    31501 catggcgggt ggacatccac tcgatggtgc ccatgcgatt aagactgtcg ctaagaccgt
    31561 tcagagagtc gcccgtgact ccccagatgc gtttcacgcc tgccgattcg agtgttttgg
    31621 cgatataagc tgcaaccgtt tgtttcatgg ttctccatct cctgaatgtg ataacggtaa
    31681 caagtttagt tcatctgacg gagggggaag ggatgggaga gaaaggaggc actaacggtt
    31741 aaatagcccg atgaaaggaa tatcatcggg cataaggcga ttatgcgaga accaaatccc
    31801 cctgcggatg gcaggagcag gccagtacgt aaccttcagc gatttcggcg tcggtcagcg
    31861 tcattgtact gctcaccgta tattcaccgg aaaccacttt tgtcttacag cagccgcaaa
    31921 cacccgcacg gcaggcagcg acaaccggaa cgttattgct ttccagcgcc tccagtagcg
    31981 tggtgccaac cggggcgtaa aattctcgtg ccggttgcag tttggtgaat ttcagaccgc
    32041 tggtcgccgc ttctgctact ggggtgaaga atttctcttt aaagaaacgc gtcacgccga
    32101 gcgctttcac ttcctgctct acccaatcca tatacggagc cgggccgcag gtcatcacgg
    32161 tacgtgaagc taagtcaggt acatctgcca gcagttcgcg agtgaggcga ccagcgataa
    32221 agccttcggt aacgttattt tctgccacca gcgttaccgg atagttacgc cactcatcgg
    32281 cgaaaataac atcctgcggc gtacgcacgt tgtagatcac ccgcacatcg gcctgtggac
    32341 ggttcttcgc aagccagcga cgcatcgaca taatcggcgt gacgccgcag cctgccgcca
    32401 gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa ttcccccatc gcgtccgaaa
    32461 gccagagata atcaccgcgt tttacatcgc gcgtcagcca ctgggagccg acaccgtcat
    32521 caatccgccg cacggtcagg gtgatatatt cactcacgcc tggcgtggag gaaatggtgt
    32581 aagcacgcag cgtttccgct gagtttcgca cgctgaccag tgcatattgc ccggcgcgat
    32641 atgggtagtg atcgtggcaa atcagggaaa tcgtccacac atccggcgtt tcttgcgtaa
    32701 tgtgatgaac ctgtatccgc cacgggcatt gattcgttgg catcgtcatc gacaaactcc
    32761 ttacgcgttc aacagttgct tcatgtcttc ttcaacagtg gtgatagaac gcaggccgaa
    32821 tttctcgttc agcaccgcca gcaggtctgg tgtcaggaaa ccaggtgcag tcgggccggt
    32881 gacgatattt ttcacgccca gagaaagcag cgtcagcaga atgacaatcg ctttctgttc
    32941 aaaccaggag agcaccagcg acagcggcag atcgttcaca ccacagccca gtttctctgc
    33001 cagagtgaca gccagaataa ttgctgagta agcatcgtta cattgacctg catctaccag
    33061 acgcggcaga ccttcgatat cgccaaactc aagtttgtta aagcgatatt taccacaggc
    33121 gagggtcagg atcaggcagt catccggcac gctggtggcg aaatcggtga agtagtggcg
    33181 ctcgccgcgt gcgccgtcac agccaccaag caggaagata tgacgcagtt tttcacggct
    33241 aaccagatca atcagcgtat cggctgcacc aagcagcgtc tggcgaccaa agcccacggt
    33301 gatcaggtga ggaatttcgc tgtacgggaa gcccgccatt tgttgcgcct gggcgatgac
    33361 cgcagagaaa tcttcgccat ccagatgacg cacgccaggc cagccaacga tgctgcgggt
    33421 ccagatacga tcgtcataag cgcctacggt tgggtcgatg atgcagttcg aggtcatcac
    33481 gatggggcca gggaaacgag cgaactccac ttgctgattc tgccagccgc tgccgtagtt
    33541 accgaccaga tgcttgaatt tacgcagctc cgggtagcca tgcgcaggca gcatttcgcc
    33601 gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc agcaggttgt agagatcttt
    33661 gagatcgtga ccggaaatca gaatgcattt acccgccgtc gctttgacgt tgacctgggt
    33721 tggcgtcggg tgaccgtatt taccggtttc gcctgcatcc agaatgctca tcactttgaa
    33781 gttcatctgg ccgatttcca ttgaacactc aagaagcgcg ttcatatcgg caggccaggt
    33841 ccccagccac gccatgattt tatggtactg ggcataaata tcgttgtcgt attgaccgag
    33901 aacatgcgcg tgttccatat aggccgccgc acctttcagg ccatacaggc acagcagacg
    33961 caggccgaga atgttttcgc caatcgccgc tttatctttg ttaggggtaa attctgctgc
    34021 ctgacgttgc agctcgccga gatcatcgct caccagttgc aggtcagcca tcgggttatc
    34081 gacgcgcgcg ttggcatcta cagccaggca ttgtgctttc agcgcctcgc gcaaggcaat
    34141 cgcttcacga gcgtagccga caatacgcgg agaatcgaag ttaacgttgg tcagggttga
    34201 gaaaaaggca cgtggcgcga agctgtcaac atcgtggttg atgatgccgt attcacgcgc
    34261 ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg agtaaatcct gaaggtcaga
    34321 agtttccgcc gttttaccac acatcccctg cgcgtatgag cagccgtttt ctgccggagt
    34381 acggatagtt tgttcacatt gcacacaaaa catgatcaca ccttttaaag ttatatttaa
    34441 tatacatgtt taagggtaag acgcttaacg cggggataaa agggattttt catgcaactt
    34501 taagggagat tgatttagcg caattttggc ggcagggaac taccgccaga gaggtattac
    34561 gcagagaaaa aggcgatgag gatcggcact aacaggctaa gaataaaacc gtgaacaatt
    34621 gccgccggga ccatatccag cccgccagta cgttgaagaa cgggcagggt gaaatccatt
    34681 gatgtggcac cgcataagcc cagtgcagta gagcggctgc ggcgaatcag cccagggatc
    34741 aacataatag caatcagttc acgggccaga tcattaaaaa acgccgcgct cccgattacc
    34801 ggaccaaaag attcggtcaa taaaataccg gaaagagaat accagccgaa accggaggcc
    34861 attgccagcg cggtattgat ggggagatca agaataaagg cgttaattaa accaccaatt
    34921 aatgaactga caaccaccac cacggcgaca atcattcccc ggcgattaag gacaatctgc
    34981 tttaaggtca tgccattatt gcgcaactga ataccaacga ggaaaagtag caaaattaac
    35041 gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca gtccactcag accaatggca
    35101 aaaccaatca ctactacgcc gcacagtttt agcgactcca gcgccatcgc aatacgcgac
    35161 gggagttttt cttgctgatg gtggttgcgc cacggcaggc ctcgctccag ccacatcagg
    35221 gcggcaatat tacacagtaa aataacggta atactgacgg cagaataatg cagaatcgcc
    35281 aacaggttac tggcgaggtt atcgagaaac gccagactga tacccataaa aaagagaata
    35341 aggtaaacca tccagcttaa tagctgatta atagctttta acgcagcttg ttggcgaagc
    35401 ggaatgaggt aacccacaat caggggaacc agaatgatta acagcccaga aaacatgaaa
    35461 atccagtcct tgcaaagaag aagtcgaaat gcgcgatgac acactactga aagcggaagg
    35521 gcgagtaaag ttgcaattaa aaggaaatgt tatgcatcag aggggg
//
LOCUS       DAJTRM010000030        35306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRM010000030 DAJTRM010000000
VERSION     DAJTRM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.030"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1140)
                     /gene="setB"
                     /locus_tag="OO322_001629"
     CDS             complement(<1..1140)
                     /gene="setB"
                     /locus_tag="OO322_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5906555.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMI"
     gene            1508..2638
                     /gene="fruB"
                     /locus_tag="OO322_001630"
     CDS             1508..2638
                     /gene="fruB"
                     /locus_tag="OO322_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5906556.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2638..3576
                     /gene="fruK"
                     /locus_tag="OO322_001631"
     CDS             2638..3576
                     /gene="fruK"
                     /locus_tag="OO322_001631"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5906557.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3593..5284
                     /gene="fruA"
                     /locus_tag="OO322_001632"
     CDS             3593..5284
                     /gene="fruA"
                     /locus_tag="OO322_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5906558.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5708..6649
                     /locus_tag="OO322_001633"
     CDS             5708..6649
                     /locus_tag="OO322_001633"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5906559.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6637..7575
                     /gene="psuG"
                     /locus_tag="OO322_001634"
     CDS             6637..7575
                     /gene="psuG"
                     /locus_tag="OO322_001634"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5906560.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7669..8919
                     /locus_tag="OO322_001635"
     CDS             7669..8919
                     /locus_tag="OO322_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5906561.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8990..9684)
                     /gene="yeiL"
                     /locus_tag="OO322_001636"
                     /pseudo
     CDS             complement(8990..9684)
                     /gene="yeiL"
                     /locus_tag="OO322_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9815..10756
                     /gene="rihB"
                     /locus_tag="OO322_001637"
     CDS             9815..10756
                     /gene="rihB"
                     /locus_tag="OO322_001637"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5906562.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10856..12106
                     /locus_tag="OO322_001638"
     CDS             10856..12106
                     /locus_tag="OO322_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5906563.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12162..13250)
                     /locus_tag="OO322_001639"
     CDS             complement(12162..13250)
                     /locus_tag="OO322_001639"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5906564.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13253..14110)
                     /gene="nfo"
                     /locus_tag="OO322_001640"
     CDS             complement(13253..14110)
                     /gene="nfo"
                     /locus_tag="OO322_001640"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5906565.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14184..15233)
                     /locus_tag="OO322_001641"
     CDS             complement(14184..15233)
                     /locus_tag="OO322_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5906566.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15332..16213
                     /locus_tag="OO322_001642"
     CDS             15332..16213
                     /locus_tag="OO322_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5906567.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16419..17888
                     /gene="lysP"
                     /locus_tag="OO322_001643"
     CDS             16419..17888
                     /gene="lysP"
                     /locus_tag="OO322_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5906568.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18180..20171
                     /gene="cirA"
                     /locus_tag="OO322_001644"
                     /pseudo
     CDS             18180..20171
                     /gene="cirA"
                     /locus_tag="OO322_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20203..21038)
                     /gene="yeiG"
                     /locus_tag="OO322_001645"
                     /pseudo
     CDS             complement(20203..21038)
                     /gene="yeiG"
                     /locus_tag="OO322_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21296..21964
                     /gene="folE"
                     /locus_tag="OO322_001646"
     CDS             21296..21964
                     /gene="folE"
                     /locus_tag="OO322_001646"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5906569.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21981..23057
                     /locus_tag="OO322_001647"
     CDS             21981..23057
                     /locus_tag="OO322_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5906570.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23199..24239
                     /gene="galS"
                     /locus_tag="OO322_001648"
     CDS             23199..24239
                     /gene="galS"
                     /locus_tag="OO322_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5906571.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24519..25517
                     /gene="mglB"
                     /locus_tag="OO322_001649"
     CDS             24519..25517
                     /gene="mglB"
                     /locus_tag="OO322_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5906572.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25578..27098
                     /gene="mglA"
                     /locus_tag="OO322_001650"
     CDS             25578..27098
                     /gene="mglA"
                     /locus_tag="OO322_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5906573.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27114..28124
                     /gene="mglC"
                     /locus_tag="OO322_001651"
     CDS             27114..28124
                     /gene="mglC"
                     /locus_tag="OO322_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5906574.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28160..29373)
                     /gene="preA"
                     /locus_tag="OO322_001652"
                     /pseudo
     CDS             complement(28160..29373)
                     /gene="preA"
                     /locus_tag="OO322_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29367..30605)
                     /gene="preT"
                     /locus_tag="OO322_001653"
     CDS             complement(29367..30605)
                     /gene="preT"
                     /locus_tag="OO322_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5906575.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30799..31038)
                     /locus_tag="OO322_001654"
     CDS             complement(30799..31038)
                     /locus_tag="OO322_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5906576.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31041..31760)
                     /gene="sanA"
                     /locus_tag="OO322_001655"
     CDS             complement(31041..31760)
                     /gene="sanA"
                     /locus_tag="OO322_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5906577.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31910..32794)
                     /gene="cdd"
                     /locus_tag="OO322_001656"
     CDS             complement(31910..32794)
                     /gene="cdd"
                     /locus_tag="OO322_001656"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5906578.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32924..33619)
                     /locus_tag="OO322_001657"
     CDS             complement(32924..33619)
                     /locus_tag="OO322_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5906579.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33616..34014)
                     /locus_tag="OO322_001658"
     CDS             complement(33616..34014)
                     /locus_tag="OO322_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5906580.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34253..35200
                     /gene="dusC"
                     /locus_tag="OO322_001659"
     CDS             34253..35200
                     /gene="dusC"
                     /locus_tag="OO322_001659"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5906581.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         8681 a         8619 c         9321 g         8685 t
ORIGIN      
        1 aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg cgacgatgcc
       61 cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga gcgtggtggc
      121 tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc cgcccagaat
      181 gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg aatgcgccat
      241 cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta agaaacgctt
      301 acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca gcccggcggc
      361 ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga taataaatag
      421 cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa tgacaaacag
      481 cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg ccagcggaag
      541 ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa taaacgctac
      601 cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat aagccagcgg
      661 tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga acatcaccgc
      721 ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg ggttagcggt
      781 cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt tgcgattcca
      841 agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca gcgatttgcg
      901 atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc caatgacagc
      961 gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat cggtaaggaa
     1021 aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg caacgattaa
     1081 aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg agttatgcat
     1141 gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag aaaataaaca
     1201 gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt gctgattggc
     1261 tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca attaggaaaa
     1321 atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc tttccttgcg
     1381 tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg tctttaaccc
     1441 taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc agcaagagag
     1501 gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg ccggagacaa
     1561 agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg tagcagaagg
     1621 ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg gcaatggtat
     1681 tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg gcgttcaggt
     1741 atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg tggcaatcgg
     1801 tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc acgtactgag
     1861 cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac ttcgcgcatt
     1921 actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc tgacgctgga
     1981 tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc tgaaagaggc
     2041 gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc tgaatctcgg
     2101 acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga ttgcggtaag
     2161 ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg tgagtgtggc
     2221 gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt tgctcgacaa
     2281 taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc tgaccagcga
     2341 tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg aacacggcct
     2401 gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca gtgatattac
     2461 cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga tgaaagttgt
     2521 ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg cagatgctga
     2581 acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg gcgcataatg
     2641 agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg tttctgcccg
     2701 gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc ggcgggtaaa
     2761 ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt tggcggcttc
     2821 ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg cattgccaac
     2881 cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga aaaagacggc
     2941 gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg ggaacgcttt
     3001 gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag cggaagctta
     3061 ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg tagtcagtgt
     3121 ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa agcggcaccg
     3181 tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa actgcctgaa
     3241 atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc gcatgttgtt
     3301 atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg gatcgctaaa
     3361 ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt tggtggcctg
     3421 atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc gacagctgtt
     3481 gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca gttggccgca
     3541 atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca taatgaaaac
     3601 gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga agaccctgct
     3661 gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg acgcagaaat
     3721 ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta aaaatgtctg
     3781 gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg aagccaaagg
     3841 ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg ccagcggtcc
     3901 gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct ttatggcggc
     3961 tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg aaacccgtgg
     4021 ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg atctggtgat
     4081 tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga tgtatcgtac
     4141 ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg ttgctgaagc
     4201 aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta agaaagagag
     4261 tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga tggtcgttgc
     4321 tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta aagagccggg
     4381 tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc tgatggtgcc
     4441 ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca cgccgggtct
     4501 gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta ttattgcggg
     4561 cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc cgcagagtat
     4621 ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg tcggtctggc
     4681 gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga ctcactggct
     4741 gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg gcatgatgtg
     4801 tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg gtctgctgag
     4861 tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc caccgctggc
     4921 aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc aggaaggtgg
     4981 caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc cgtttgctgc
     5041 tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga ctggcgcaat
     5101 ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg ttctgctgat
     5161 ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg gtacgctggt
     5221 ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag cgaaagcagc
     5281 gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg atgagcgcaa
     5341 agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat ctttcagtag
     5401 aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt attttctgcc
     5461 cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt gtaattcagg
     5521 catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc tggcggtaat
     5581 ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca catgtttaaa
     5641 ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt ttaccagagg
     5701 acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata ttgatgtcgc
     5761 cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa taaaatttac
     5821 gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta acaaagcctg
     5881 gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc aaaccaatca
     5941 atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt cgagttattt
     6001 atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga atattagcaa
     6061 cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg caaaggtcat
     6121 tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata atgccgccaa
     6181 cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc gcgaccgtct
     6241 aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga gtgggattgc
     6301 gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac atggcctgaa
     6361 ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca gcggtgaaaa
     6421 tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg gcgatgccat
     6481 gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat ctgttcgttt
     6541 cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc ccgatttatc
     6601 gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa aatttcccct
     6661 gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa accggttgtg
     6721 gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc ccagaccgca
     6781 attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat cgccattatt
     6841 ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg tcgtgaaggg
     6901 cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc cggaaaaaat
     6961 ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat taaagtattt
     7021 gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga tatttctgcc
     7081 gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc gaaatctatt
     7141 ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt aattggctat
     7201 cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt cagcattcgt
     7261 ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag cgggctgaac
     7321 ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga ggaatctatc
     7381 aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat tggtaaagaa
     7441 agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag cctgaaatcc
     7501 aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa agaataccag
     7561 cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat caagcatacc
     7621 aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat ggatataatg
     7681 agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc agtgaataaa
     7741 aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc tattggtggc
     7801 atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt aggcgttcat
     7861 aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct ggttgggccg
     7921 aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt acttccggcg
     7981 attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat ggggctgctg
     8041 attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat cgaatctttt
     8101 gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt taaaccgttt
     8161 atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat ggcgtccatt
     8221 gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta cctgttagcg
     8281 gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag cccggcaacc
     8341 gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc aaaaagcttt
     8401 atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg tgtagcgacg
     8461 gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg cggaattggc
     8521 ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt gctggcaccg
     8581 ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag cctgattggg
     8641 cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata cctgcaaacg
     8701 ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg tggttttgct
     8761 aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc aaaacgtgct
     8821 ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc caacctgatg
     8881 agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg tagaccggat
     8941 aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt tactgcatca
     9001 tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac tgctttctgt
     9061 ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt gcgcgagaac
     9121 atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat gcttttcgtg
     9181 ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca ctaacggaaa
     9241 tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta aggtgacgca
     9301 aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt tcatagggag
     9361 cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt ctttgtcgat
     9421 taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg acacgcgacc
     9481 attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca gccatgacgg
     9541 ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc gcgtatcggc
     9601 tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt gctgcttaag
     9661 gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc atatttagca
     9721 tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt gctgtagaaa
     9781 aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa ttattctgga
     9841 ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac atccggcaat
     9901 agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa cattaattaa
     9961 tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg ggatgccgca
    10021 acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg gactggatgg
    10081 cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga aatatatcat
    10141 cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc cgctttcaaa
    10201 tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg aaattgtgct
    10261 aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca atatctttgc
    10321 cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga tgatgggcct
    10381 cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg aaagggtagg
    10441 cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa cgcagttcga
    10501 atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt atctgatcaa
    10561 ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca gcggcccttg
    10621 ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta ataccaaagt
    10681 cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg tgcgcggcta
    10741 catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat gcaggcccgg
    10801 ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa tcactatgga
    10861 tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt tactgtcagt
    10921 aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac aggtcgtgat
    10981 tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag tcgcttttgg
    11041 cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg gttctctggt
    11101 cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt tcagggtatt
    11161 accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg gtgtgatggg
    11221 gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca gcaagatcga
    11281 gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg cgatcgtcaa
    11341 accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta gtggcatggc
    11401 ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg tggaatattt
    11461 gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc tgttaagccc
    11521 ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa caccgccaaa
    11581 aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg ccgccggtgt
    11641 agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta ttatcggcgg
    11701 cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag gttacctgct
    11761 ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg ccgggagttt
    11821 gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct caccctatct
    11881 gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg cgttgtgcgg
    11941 tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg ttgcgccaca
    12001 ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga cactttctaa
    12061 tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg caatttatga
    12121 gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat tggcactaat
    12181 gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc gcgtgaaatt
    12241 gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc cagaaagcta
    12301 tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt cgtatgcgct
    12361 ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga ctcatcgggc
    12421 aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc ggcattacgg
    12481 tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa agtgggtttc
    12541 agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc tgaaacggta
    12601 tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc ctcggctgtc
    12661 aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga cccgttcagt
    12721 agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac ggtttgatcg
    12781 tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg aacgcagccg
    12841 gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata aaagtcatcg
    12901 ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg ggcgatattg
    12961 cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc ttgcgggtaa
    13021 tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac gcagtattcc
    13081 tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa atccataata
    13141 tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt ctgctgaatc
    13201 agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat cttcaggcta
    13261 ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag atatccgggt
    13321 tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata atccagcgga
    13381 acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg cggctgccaa
    13441 aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg acaatacggg
    13501 caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac ccggcagcga
    13561 aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg ccgtcgataa
    13621 tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg gtgttttcaa
    13681 tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg gcaatacgcg
    13741 caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag ttgagcaaag
    13801 aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc gatttttcca
    13861 gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga agaatttgcg
    13921 ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt tgcgtcgtga
    13981 gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg gttgcgtcga
    14041 tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg tgcgctccaa
    14101 tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat gatagcgggt
    14161 taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata gttaatcgca
    14221 ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg tttcgcccca
    14281 gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc cattgccagc
    14341 aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag gtggaacgag
    14401 ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat tttgcttttc
    14461 tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat gaggaacgga
    14521 gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc atccgggctg
    14581 atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc aatgtagata
    14641 ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg gtagaggaaa
    14701 atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt actggcttcc
    14761 gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact gctaccggca
    14821 ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag gaagcaagcc
    14881 agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat accgacatcg
    14941 gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg taataaatat
    15001 tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata gatggtgttg
    15061 cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc acccgcaacc
    15121 gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag ggctaacccc
    15181 ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt cataaccctc
    15241 tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg attatatatt
    15301 tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc agttggaagt
    15361 ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc tggcgttgtc
    15421 gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg ttcaactgtt
    15481 tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct atccgcgagc
    15541 gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag acaacggcgc
    15601 gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag ttatcgcccg
    15661 ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca gccaggacgt
    15721 gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac cgtgccacag
    15781 cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg ccgcgccgac
    15841 ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc cgtggatcct
    15901 gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt cacatttacc
    15961 gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg cggtgcgtca
    16021 tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc aggcaggcac
    16081 attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc gtatacatca
    16141 tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg atcccgcaaa
    16201 tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt cgtgacgcag
    16261 cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa tccctgggcg
    16321 atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat cgtctgctac
    16381 aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa actaaaacta
    16441 cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg attgccattg
    16501 gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct caggcaggtc
    16561 cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc ctgatgacca
    16621 gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact tacggtcaga
    16681 actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac aactgggcgg
    16741 tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg ttcccggata
    16801 caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg aactacatct
    16861 ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc acgacagtta
    16921 ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc gcgcagcctg
    16981 cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt gcggcgatga
    17041 tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc ggtattgctg
    17101 caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag gtattctggc
    17161 gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt ccgtataccg
    17221 atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc accctggtgt
    17281 tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt ctgacggcgg
    17341 tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac accctggcgt
    17401 gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg ccgcgtaatg
    17461 cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg tttggcaacc
    17521 agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc gcctggctgg
    17581 ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga cacgacatta
    17641 acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca ttcattctgt
    17701 gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc attgactggg
    17761 gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg ttcggctaca
    17821 agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg cagaacgata
    17881 agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct ttttcaattc
    17941 catttccctg acaaatcatg cggatataaa atttaacatt tggattgata attgttatcg
    18001 tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct gttccggctt
    18061 tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac gcaaggggac
    18121 gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg agatatggaa
    18181 tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt tcttgtgcat
    18241 ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca tcttccgtgg
    18301 aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa gacctgcagc
    18361 gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta caactgacga
    18421 acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc tacaccctga
    18481 ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac aatgatttcg
    18541 atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt ggcccgatgt
    18601 cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc aaaaaaatcg
    18661 gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat cgcgatcgcg
    18721 gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt gtgctgggaa
    18781 tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac tcaacgacca
    18841 ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc aatgtcgaat
    18901 ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc gaccgtcagg
    18961 atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac tgcgtcagcc
    19021 ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag aaagtcgaga
    19081 acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc gacggcaaat
    19141 acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa tggcgtcacg
    19201 acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg tctgccagcc
    19261 agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg ctgacgaccg
    19321 gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt gcctacatgg
    19381 tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca tttaaagcac
    19441 cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc gcatgtaaga
    19501 ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg gggctttact
    19561 acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt ttccgtaacg
    19621 atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca ccgggctacc
    19681 aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta tttagctact
    19741 acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt ccgttcaacg
    19801 atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc agcaacggcg
    19861 aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg gactggaaac
    19921 cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg cagaaacgcg
    19981 ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc ggcgcggcct
    20041 ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc gacaaggatc
    20101 tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg gcagtggatt
    20161 atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca gtaaatactg
    20221 cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac tgtgatcata
    20281 tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt ctgccaatac
    20341 cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga taagcgtcgg
    20401 gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt ccagcatgca
    20461 tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac gctgcacgga
    20521 ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt cagcgccata
    20581 atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc gctgacatta
    20641 aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat ccggtaatgc
    20701 gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc taaatcgtag
    20761 ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac cagtacaatc
    20821 cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc gtcattgcag
    20881 gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg gggggggaga
    20941 aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca gcgttgctgc
    21001 cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt gttgtgttgt
    21061 ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc acttccgtat
    21121 ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca ataattatcg
    21181 ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt aaatcagctt
    21241 aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat cataaatgcc
    21301 atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag gactggaaac
    21361 accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc ttattgctgg
    21421 tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca gtttgatgga
    21481 aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc tggattacgc
    21541 caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa tggtcaccgt
    21601 gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg atggcaaagc
    21661 gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta accgtatcgt
    21721 gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa ttcttattgc
    21781 gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg tgcattactg
    21841 cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct ctcttggtgg
    21901 attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc gccatcacaa
    21961 ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt cgcggcgtcg
    22021 ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag gcggcttatc
    22081 tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg gcatttctcg
    22141 atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt gctggcctgc
    22201 aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg ttagtcttgt
    22261 tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct tacgggctgg
    22321 tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc agccgcgcct
    22381 ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac ggcggcgttg
    22441 aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg ggcgcacagt
    22501 atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc agcggctggc
    22561 tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg
    22621 gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg gcgtatcgct
    22681 ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag gcgattggct
    22741 atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg gtgcttgcga
    22801 tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg atttgtacca
    22861 cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg ctggcgtttg
    22921 ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt tacttccgcc
    22981 aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga ccggcaatat
    23041 ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg taaccgtttc
    23101 cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata gccgccagca
    23161 agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt cgtgatgtag
    23221 cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac agcacgctgg
    23281 tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat tatcggccaa
    23341 acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg gtggtgatgg
    23401 acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc gcccagcagc
    23461 atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca
    23521 ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg
    23581 acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc aaccgcgttg
    23641 tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc cgaatggcga
    23701 cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc agccacggca
    23761 ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag caggatatta
    23821 ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt gaggcggcga
    23881 tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat aacgacaata
    23941 tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg ttacatctct
    24001 caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa ttaacgaccg
    24061 tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg cagggggcag
    24121 caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg cgtcgccatt
    24181 ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag gcgatgtaac
    24241 cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta tgattgcacc
    24301 gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag gtaatagcgt
    24361 cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa cgaattttaa
    24421 aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc gagcatttat
    24481 ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag gtgttaaccc
    24541 tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct gatactcgca
    24601 ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc aaggctattg
    24661 agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct cagaatgacc
    24721 agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag gcactggcaa
    24781 tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg
    24841 tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag
    24901 cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat ttgattgcta
    24961 aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt cagttcgtac
    25021 tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac gtgattaaag
    25081 aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca atgtgggaca
    25141 ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg
    25201 aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca ctgaaagcac
    25261 acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg
    25321 tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag gcgaaagcga
    25381 cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc accaactgga
    25441 aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac aacttggctg
    25501 aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt attgtatggc
    25561 caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa tacttgttgg
    25621 aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac gttaatttaa
    25681 aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga aaatcgacat
    25741 tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta ttccagggta
    25801 aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg atggtacacc
    25861 aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg gggcgatatc
    25921 ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg
    25981 atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa
    26041 tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg attatggatg
    26101 aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt attcgtaaat
    26161 taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa atcttccagt
    26221 tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag
    26281 gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat cagcgttttc
    26341 ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta acgtcactgc
    26401 gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc ctcggtattg
    26461 ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt attcgcgaga
    26521 aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat gccaacgaag
    26581 ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga atttatgcct
    26641 atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa aataaagttg
    26701 gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat tcgatgcggg
    26761 taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat cagcaaaagg
    26821 tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc
    26881 gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa ctggcgaaga
    26941 aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg ataacagacc
    27001 gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa acaacaacgc
    27061 aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt cccatgagtg
    27121 cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac gtcgttcttt
    27181 tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg ttgaacttaa
    27241 gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa
    27301 ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg gcagtggtgg
    27361 ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa atggcgacga
    27421 tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc ggtttgatca
    27481 acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc ggcacgatga
    27541 tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg ccaatttctg
    27601 gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg agtttccgtc
    27661 tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca
    27721 aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg gcaaaaatat
    27781 ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc gtgttctatg
    27841 cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac ctcggcttta
    27901 tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg
    27961 tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc aactatggcc
    28021 tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg attattatct
    28081 tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat tgcggatatt
    28141 gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct ttcttaaact
    28201 tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc agacaaccca
    28261 cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc atcgcctggt
    28321 gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg tcaagattga
    28381 tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata ttgttattcg
    28441 ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa tgcgataacc
    28501 cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg gtgacctgta
    28561 acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca atgccgccga
    28621 taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga atgaagcgca
    28681 gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta ccgttaacga
    28741 tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg gtgttaattg
    28801 ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac atatcaccga
    28861 tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact gcccgacaat
    28921 atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga gaagtcattt
    28981 gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt gctgctcatt
    29041 ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca gccgacgcag
    29101 ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt tgaagccgat
    29161 aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt tggcgataaa
    29221 aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt tggcacacat
    29281 ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa agttcacgcc
    29341 gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa atagtgatga
    29401 atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt gtcaccctca
    29461 acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg tgttttgatg
    29521 gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg accgacggcg
    29581 agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg cttaaacgtg
    29641 actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac gcccagctcc
    29701 cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc acgtgctacg
    29761 caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat cgcgacatca
    29821 ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc ctgtcgtaca
    29881 cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg tagcccggaa
    29941 ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag ttgctccagg
    30001 gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt ttcaatacgg
    30061 gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc gttacgcaac
    30121 cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg gtttgtcagt
    30181 gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt gccgagcgtt
    30241 ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt aacaaaacgc
    30301 tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc gctttggcat
    30361 aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt ttcgcgaatt
    30421 gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt ccccggatcg
    30481 gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca gcgagaggct
    30541 tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata attttgttgc
    30601 ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata aatattgcgt
    30661 gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc acaaatagct
    30721 gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt ctcaataatt
    30781 aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa
    30841 aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat tttttgctcg
    30901 ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca
    30961 gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa
    31021 aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg
    31081 ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg
    31141 accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg
    31201 aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca
    31261 taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc acagtggaaa
    31321 cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa
    31381 tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg
    31441 attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt atcgccgctc
    31501 agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag
    31561 cgataatact gattaataac gccggtacga taatattttg ctgtgccgag caccacgccg
    31621 acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc
    31681 atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg
    31741 aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca tcaggctact
    31801 ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga
    31861 gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa
    31921 gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact
    31981 gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat
    32041 aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc gggttgaatg
    32101 cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc agcgcgacac
    32161 cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag
    32221 aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa
    32281 tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc
    32341 ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc
    32401 agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca ccactcaacc
    32461 aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt gcaccaataa
    32521 attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat
    32581 taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca
    32641 gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca
    32701 aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa
    32761 gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg
    32821 gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca
    32881 acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc attactgcca
    32941 gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg
    33001 ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg
    33061 cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat
    33121 taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc
    33181 tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg
    33241 atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag
    33301 tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc
    33361 gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt
    33421 gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag atgccggtca
    33481 gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact
    33541 tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc
    33601 accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg
    33661 taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga
    33721 aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc
    33781 aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca
    33841 tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct
    33901 gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata
    33961 aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa
    34021 caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat
    34081 taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag cgaactcagg
    34141 catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc agacctttaa
    34201 tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg atatgcgtgt
    34261 attactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt
    34321 taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt
    34381 aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac catctggtac
    34441 gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg
    34501 tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt
    34561 taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct accagggggc
    34621 aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag tgcgtctggg
    34681 ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggcgctac
    34741 ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc atattgactg
    34801 gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca acggtgaaat
    34861 ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat
    34921 tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata acgaaccgcg
    34981 aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg
    35041 cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga
    35101 atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact cccctgatat
    35161 tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt gatgtttttg
    35221 acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc aaaatcgccg
    35281 aggcgttccc tgaaggccgg ggcagc
//
LOCUS       DAJTRM010000031        34892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRM010000031 DAJTRM010000000
VERSION     DAJTRM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.031"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            847..3132
                     /gene="nrdA"
                     /locus_tag="OO322_001660"
     CDS             847..3132
                     /gene="nrdA"
                     /locus_tag="OO322_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5906582.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3366..4496
                     /gene="nrdB"
                     /locus_tag="OO322_001661"
     CDS             3366..4496
                     /gene="nrdB"
                     /locus_tag="OO322_001661"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5906583.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4496..4750
                     /gene="yfaE"
                     /locus_tag="OO322_001662"
     CDS             4496..4750
                     /gene="yfaE"
                     /locus_tag="OO322_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5906584.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4804..5454)
                     /gene="inaA"
                     /locus_tag="OO322_001663"
     CDS             complement(4804..5454)
                     /gene="inaA"
                     /locus_tag="OO322_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5906585.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5633..5875
                     /locus_tag="OO322_001664"
                     /pseudo
     CDS             5633..5875
                     /locus_tag="OO322_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5917..6993)
                     /gene="glpQ"
                     /locus_tag="OO322_001665"
     CDS             complement(5917..6993)
                     /gene="glpQ"
                     /locus_tag="OO322_001665"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5906586.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6998..8356)
                     /gene="glpT"
                     /locus_tag="OO322_001666"
     CDS             complement(6998..8356)
                     /gene="glpT"
                     /locus_tag="OO322_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5906587.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8629..10257
                     /gene="glpA"
                     /locus_tag="OO322_001667"
     CDS             8629..10257
                     /gene="glpA"
                     /locus_tag="OO322_001667"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5906588.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10247..11506
                     /gene="glpB"
                     /locus_tag="OO322_001668"
     CDS             10247..11506
                     /gene="glpB"
                     /locus_tag="OO322_001668"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5906589.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11503..12693
                     /gene="glpC"
                     /locus_tag="OO322_001669"
     CDS             11503..12693
                     /gene="glpC"
                     /locus_tag="OO322_001669"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5906590.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12886..13836
                     /locus_tag="OO322_001670"
     CDS             12886..13836
                     /locus_tag="OO322_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5906591.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13877..14347)
                     /locus_tag="OO322_001671"
     CDS             complement(13877..14347)
                     /locus_tag="OO322_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5906592.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14402..15604)
                     /locus_tag="OO322_001672"
     CDS             complement(14402..15604)
                     /locus_tag="OO322_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5906593.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15691..16233)
                     /locus_tag="OO322_001673"
     CDS             complement(15691..16233)
                     /locus_tag="OO322_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906594.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16512..16937
                     /gene="nudI"
                     /locus_tag="OO322_001674"
     CDS             16512..16937
                     /gene="nudI"
                     /locus_tag="OO322_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5906595.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16976..17578)
                     /locus_tag="OO322_001675"
     CDS             complement(16976..17578)
                     /locus_tag="OO322_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5906596.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17886..19025
                     /gene="arnB"
                     /locus_tag="OO322_001676"
     CDS             17886..19025
                     /gene="arnB"
                     /locus_tag="OO322_001676"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5906597.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19029..19997
                     /gene="arnC"
                     /locus_tag="OO322_001677"
     CDS             19029..19997
                     /gene="arnC"
                     /locus_tag="OO322_001677"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5906598.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19997..21978
                     /gene="arnA"
                     /locus_tag="OO322_001678"
                     /pseudo
     CDS             19997..21978
                     /gene="arnA"
                     /locus_tag="OO322_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21975..22865
                     /locus_tag="OO322_001679"
     CDS             21975..22865
                     /locus_tag="OO322_001679"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5906599.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22865..24517
                     /gene="arnT"
                     /locus_tag="OO322_001680"
     CDS             22865..24517
                     /gene="arnT"
                     /locus_tag="OO322_001680"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5906600.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24514..24849
                     /gene="arnE"
                     /locus_tag="OO322_001681"
     CDS             24514..24849
                     /gene="arnE"
                     /locus_tag="OO322_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5906601.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24849..25235
                     /gene="arnF"
                     /locus_tag="OO322_001682"
     CDS             24849..25235
                     /gene="arnF"
                     /locus_tag="OO322_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5906602.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25229..25501)
                     /gene="pmrD"
                     /locus_tag="OO322_001683"
     CDS             complement(25229..25501)
                     /gene="pmrD"
                     /locus_tag="OO322_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5906603.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25606..26961)
                     /gene="menE"
                     /locus_tag="OO322_001684"
     CDS             complement(25606..26961)
                     /gene="menE"
                     /locus_tag="OO322_001684"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5906604.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26958..27920)
                     /gene="menC"
                     /locus_tag="OO322_001685"
     CDS             complement(26958..27920)
                     /gene="menC"
                     /locus_tag="OO322_001685"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5906605.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     SSPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27920..28777)
                     /gene="menB"
                     /locus_tag="OO322_001686"
     CDS             complement(27920..28777)
                     /gene="menB"
                     /locus_tag="OO322_001686"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5906606.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28792..29550)
                     /gene="menH"
                     /locus_tag="OO322_001687"
     CDS             complement(28792..29550)
                     /gene="menH"
                     /locus_tag="OO322_001687"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5906607.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29547..31217)
                     /gene="menD"
                     /locus_tag="OO322_001688"
     CDS             complement(29547..31217)
                     /gene="menD"
                     /locus_tag="OO322_001688"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5906608.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31306..32601)
                     /gene="menF"
                     /locus_tag="OO322_001689"
     CDS             complement(31306..32601)
                     /gene="menF"
                     /locus_tag="OO322_001689"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5906609.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32680..32985)
                     /gene="elaB"
                     /locus_tag="OO322_001690"
     CDS             complement(32680..32985)
                     /gene="elaB"
                     /locus_tag="OO322_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5906610.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33040..33501)
                     /locus_tag="OO322_001691"
     CDS             complement(33040..33501)
                     /locus_tag="OO322_001691"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5906611.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33566..34483
                     /gene="rbn"
                     /locus_tag="OO322_001692"
     CDS             33566..34483
                     /gene="rbn"
                     /locus_tag="OO322_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5906612.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34674..34862
                     /locus_tag="OO322_001693"
                     /pseudo
     CDS             34674..34862
                     /locus_tag="OO322_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8534 a         9338 c         8645 g         8375 t
ORIGIN      
        1 aaaggctttg cctgctggcg tgacaactcg ctttgatatc atatccctgg cataaatcaa
       61 tggccgccgc gacaccgtta gcagagaaaa gagatgcaat cattgacggg agtagagata
      121 aatactcctt gcgtagaaag ataatccgca tattaatctg accatcattt tatataagac
      181 ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt gtccaacccg
      241 atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata attcaagtta
      301 ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat aattttctta
      361 taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat agaaaacaac
      421 catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact gatacatcag
      481 attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa gattaataaa
      541 ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga acagttattt
      601 ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc taaggtgcgc
      661 gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa gagggtcatt
      721 ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt ctaagcagct
      781 tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa aacaggtacg
      841 acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga gcgcatcaat
      901 ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa cgtttcgatt
      961 tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac ctctgacatc
     1021 cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc ggattatcag
     1081 tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg ccagtttgag
     1141 ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata cgataatcat
     1201 ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat cgatcacgac
     1261 cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata tctagtacag
     1321 aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct tgttgccgcg
     1381 tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg tttttatgac
     1441 gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt gcgtaccccg
     1501 actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga ttccatcaac
     1561 gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg catcaacgcc
     1621 ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca taccggctgt
     1681 atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg cggtgtgcgc
     1741 ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag cctgctggtg
     1801 ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta cggggtacaa
     1861 atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct gttcagcccg
     1921 tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt tgaacgcctg
     1981 tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc cgttgagctg
     2041 ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca gaacgttgac
     2101 cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca gtctaacctg
     2161 tgcctagaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga gaacggtgaa
     2221 atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct ggatgaactg
     2281 gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta tcaggattac
     2341 ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat tggtgtgatt
     2401 aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag cgccaacaac
     2461 ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc taatgagctg
     2521 gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa agggatcctg
     2581 ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct gcattacgac
     2641 tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac gctttctgcc
     2701 ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat tgaaccgccg
     2761 cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt ggtgccggac
     2821 tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa cgatggctac
     2881 ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc taacaccaac
     2941 tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct gaaagacctg
     3001 ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg tgacggcgct
     3061 gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga aagcggcgca
     3121 tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg cctacggctc
     3181 ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc cggatgcagc
     3241 gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg cgccagcgtc
     3301 gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc caacaggaca
     3361 cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa gaaccgatgt
     3421 tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac atcttcgaaa
     3481 agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac gtctcccgcg
     3541 accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc agcaacctga
     3601 aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg ctattgccgc
     3661 ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca gaaacgattc
     3721 attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct gttgtgtttg
     3781 acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc agctattacg
     3841 atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc cacaccgtta
     3901 acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat ctctgcctga
     3961 tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt tcctttgcct
     4021 ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt gcccgcgatg
     4081 aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc ggcgcggatg
     4141 atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac ctgtttgtcc
     4201 aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt tcgatgattg
     4261 gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc cgcatgcagg
     4321 cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg atcaacacct
     4381 ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt tcttatctgg
     4441 tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag ctctgatggc
     4501 ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac acccttccct
     4561 tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg aaggttactg
     4621 cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg aaccgttagc
     4681 ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg atattgaaat
     4741 cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc tggcctacaa
     4801 cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag ggatcggctc
     4861 cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac gcaagcggcg
     4921 acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt tcacatagat
     4981 atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct taaacgccag
     5041 tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata ctgcatgccg
     5101 ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca gcaacgcgcg
     5161 ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga tgacaccggc
     5221 ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc cgaacggata
     5281 acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt tgccgttgcg
     5341 ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt caacccagtc
     5401 gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg ccatacccta
     5461 tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga aaatcttaat
     5521 gaaacgtgtc gtattaatga atttcttttg ccgctacagg tgtgacaccg cttttgccct
     5581 tcatttgcag cgcatagatt ccaccagcca ctaccactaa tagtgcgccc agcagataaa
     5641 atgtgaattt aatcagtcgg atacagcgat gaattttatt cgacagggat taggcatcgc
     5701 cttacaacca gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg ttcctcacga
     5761 accaaccttc tatcgacaga tttcgttgct gactaaagaa aagccggtag aaggcagtcc
     5821 actgttttta ctacaaatgt gcatggaaca attagtggcg attggaaaaa tttgatatga
     5881 gacaggatgg cgcatgaacg ccatcctgca gtaacattac tctttattaa gaaattttac
     5941 tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat acagagcatc
     6001 atataactga ttcacatcag gagtgtattc aggcagttta tctgaccgca cggtataagg
     6061 atgcactacc agtttattct gctgggcatc ttgcaccatg ccagtgagtt tgatattacc
     6121 cggctgcgat gtctcctcaa tcaacatatg gtaatccgga ccaataccat ctgcatattc
     6181 cgccacctgt ttcatggctc ccggcttaaa catccagtcg tagttgtaat taacccagct
     6241 tccatccggc tgtttctgct gcgtttcatt ccagtcggta taggcaatca gctgtaccag
     6301 attgagctcc atgcccattt tgggttccag ctcattctta atacgcttca gctcatcagc
     6361 atcaaaacat tgcaaataaa ctttatcgtc tttaccggtg taaccatatt tcttcagcac
     6421 ttccagcgtt tttgccgcaa tatccttccc ttcctgatga tggaaccacg gcgctttgat
     6481 ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa caaattcaat
     6541 ctcttcttca aaggtgtgca cccggaagtc ggacttaccc attgggaaac gccccggata
     6601 agtctgtact tttttaccgt tttcaatatc gaaaccttcg gtaaatttca gcgacttaat
     6661 ttcatccagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc gatccgggaa
     6721 acgatcggca acatcagtaa cacgatcgag ataatggtca tgcagaacaa ccagatggtc
     6781 gtctttggtc atcaccaaat cctgttccag ataatccgct ccctgcgcat acgccatcgc
     6841 ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg cgatgactat
     6901 tttttcgttg ctgtccgccg ccattgcact gcttcccatg actatagtgc tcatcatgat
     6961 cgccatgcta aggtttttca gcttcaattt catgccatta gcctccgttg cgtttttgca
     7021 gtaattgttc atggcgacgt ttttcgccaa tcatcacaac aatcaacaag ataaccgcca
     7081 gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag tccacggtgt
     7141 agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc ccggtaaagc
     7201 ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga ccgatcagca
     7261 tcacaggacc gtagatcagg aagccgataa caatcataca aatcatatcg acggttgggt
     7321 tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc ataaagaaga
     7381 cgccggttgc cccacggtta ccacggaaga ctttgtccga catccagccg cacagcaggg
     7441 tgcccggaat acccgcatac tcatagaaga agtaggccca ggaggattta tccagcgcga
     7501 aatgcttaac ctctttcaga taagtcggtg accagtcgag gatgccgtaa cgcagcagat
     7561 aaacgaacac gttggcgatg gcgatgtacc acagcagttt gttcggcagc acgtactgca
     7621 tgaagatttg cttcgcggtc agctcctgtt ccgctttttc gttatagtcg tccggataat
     7681 catttttgta ctcttcgatc ggcggcaagc cacaggattg cggggtatcg cgcatcatcg
     7741 caaaggcgaa taatgccacc agaatggcgc agaaagcagg catatagagc gccgcatgcc
     7801 agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca ccaccgacgt
     7861 tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac caccagtgca
     7921 ccatagtacg accacacggc ggccacccca tcccctggaa ccaaccgcag aggaacaaca
     7981 gtacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata aacaacatca
     8041 ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag cgatccgata
     8101 ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa agggcaaaac
     8161 ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcaagc gcaaagttct
     8221 tacgaaccaa atagtaagcc gcatagccaa agaatatccc caggaaaatt tgccagcgca
     8281 atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttgtgt ggcgctggtt
     8341 taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga ttaacagcct
     8401 gattcagtga gagaacctgc cgtttcttga gttgccgcga tgttaagaaa acattcataa
     8461 attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt caaaatgacg
     8521 catgaaatca cgttttactt tcgaatcatg agcgaatatg cgcgaaatca aacaattcat
     8581 gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat gaaaactcgc
     8641 gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc cgggattgcc
     8701 cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga catcgcaacc
     8761 ggtgccaccg ggcgtaacca cggcctgctg cacagcggtg cgcgctatgc agtaaccgat
     8821 gcggaatcgg cccgcgaatg cattagtgaa aaccagatcc tgaaacgcat tgcacgtcac
     8881 tgcgttgaac caaccaacgg cctgtttatc accctgccgg aagatgacct ctcattccag
     8941 gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat agacccgcag
     9001 caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt gaaagttccg
     9061 gatggcaccg ttgatccatt tcgtctgacc gcagcaaaca tgctggatgc caaagaacac
     9121 ggtgccgtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg cgcgacggtg
     9181 tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaactc aggcccttca tgcacctgtc
     9241 gtggttaatg ccgctgggat ctgggggcaa cacattgccg aatatgccga tctgcgcatt
     9301 cgcatgttcc cggcgaaagg atcgctgctg atcatggatc accgcattaa ccagcatgtg
     9361 atcaaccgct gccgtaaacc ttccgacgcc gatattctgg tgcctggcga taccatttcg
     9421 ctgattggta ccacctcttt acgtattgat tacaacgaga ttgacgataa tcgagtgacg
     9481 gcagaagagg ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt gatggcgaaa
     9541 acgcgcattt tgcgggccta ttctggcgtg cgcccgctgg ttgccagcga tgacgacccg
     9601 agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg cgatggtctg
     9661 gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat ggctgaatgg
     9721 gctaccgacg cggtatgccg caaactgggc aacacgcgcc cctgtacgac tgccgatctg
     9781 gcactgcctg gttcacaaga acccgctgaa gttaccttgc gtaaagtcat ctccctgcct
     9841 gccccgctgc gcggttctgc ggtttatcgt catggcgatc gcacgcctgc ctggctgagc
     9901 gaaggccgtc tacaccgtag cctggtatgt gagtgcgaag cggtaactgc gggtgaagtg
     9961 cagtacgcgg tagaaaattt aaacgttaat agcctgctgg atttacgccg tcgtacccgt
    10021 gtggggatgg gcacctgcca gggcgaactc tgcgcctgcc gcgctgccgg actgctgcaa
    10081 cggtttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt ccttaacgaa
    10141 cgctggaaag gcgtgcaacc catcgcctgg ggagacgcac tgcgcgaaag cgaatttacc
    10201 cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc gctttgatac
    10261 tgtcattatg ggcggcggcc tcgccggatt actctgtggc ctgcaactgc aaaaacacgg
    10321 cctgcgctgt gccattgtca cgcgtggtca aagcgcgttg catttctcat ccggttcgct
    10381 ggatttgctg agccatctgc cagatggtca acccgtggca gacattcaca gtggactgga
    10441 atctttgcgt cagcaggcac ctgcacatcc ttactctctt ctcgggccac aacgcgtgct
    10501 cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat tgcagggcag
    10561 cgtagaactg gctcaccagc ggattacgcc gctcggcact ttgcgtgcaa cctggttaag
    10621 ttcgccagaa gttcccgtct ggccgctacc cgcgaagaag atatgtgtag tggggataag
    10681 cgggctgatg gatttccagg cgcatcttgc ggcggcttcg ttgcgcgaac tggatctttc
    10741 ggttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata acgccaccga
    10801 atttcgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc cgctgttact
    10861 tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg cctgcttcgg
    10921 cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt cactgatgct
    10981 tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt tacagcgcca
    11041 gtttgtgcgc caaggtggag tgtggatgcc gggcgatgaa gtgaaaaaag tgacctgtaa
    11101 aaatggcgta gtgaatgaaa tctggacccg caatcacgcc gatattccgc tacgtccacg
    11161 tttcgcggtt ctcgccagcg gcagtttctt tagtggcgga ctagtagcgg aacgtaacgg
    11221 cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg gtgaatggta
    11281 taagggcgat ttttttgcgc cgcaaccgtg gcagcagttc ggtgtaacca ctgatgagac
    11341 gctacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg gttcggtgct
    11401 gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg tcagtgcttt
    11461 acatgccgct caacagattg cccaacgcgc aggaggccaa caatgaacga caccagcttc
    11521 gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg ggtgaatccc
    11581 ggttatccag ggccaaaaca agccgggccg gatggcgagc gtctgcgttt gaaagatggc
    11641 gcactgtatg acgaggcgct gaaatattgc atcaactgca aacgttgtga agtcgcctgc
    11701 ccgtccgatg tgaagattgg cgatattatc cagcgcgcgc gggcgaaata tgacaccacg
    11761 cgcccgtcgc tgcgtaattt tgtgttgagt cataccgacc tgatgggtag cgtttccacg
    11821 ccgttcgcac caatcgtcaa caccgctacc tcgctgaaac cggtgcggca gctgcttgat
    11881 gcggcgttaa aaatcgatca tcgtcgcacg ctaccgaaat actccttcgg cacgttccgt
    11941 cgctggtatc gcagcatagc ggctcagcaa gcacaatata aagaccaggt cgctttcttt
    12001 cacggctgct tcgttaacta caaccatccg cagttaggta aagatttaat taaagtgctc
    12061 aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg cgtaccgcta
    12121 atcgccaacg gctttaccga taaagcacgc aaacaggcaa ttacgaatgt agagtcgatc
    12181 cgcgaagctg tgggagtaaa aggcattccg gtgattgcca cctcctcaac ctgtacattt
    12241 gccctgcgcg acgaataccc ggaagtgctg aatgtcgaca acaaaggctt gcgcgatcat
    12301 atcgaactgg caacccgctg gctgtggcgc aagctggacg aaggcaaaac gttaccgctg
    12361 aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa aatgggctgg
    12421 acgctctaca ccctggagct gttgcgtaac atcccggggc ttgagttaac ggtgctggat
    12481 tcccagtgct gcggtattgc gggtacttac ggtttcaaaa aagagaacta ccccacctca
    12541 caagccatcg gcgcaccact gttccgccag atagaagaaa gcggcgcaga tctggtggtc
    12601 accgactgcg aaacctgtaa atggcagatt gagatgtcca caagtcttcg ctgcgaacat
    12661 ccgattacgc tactggccca ggcgctggct taaactccct tctgatgccc ggtaagcatg
    12721 tggttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt gcaacgcagt
    12781 gaaaattcct ctgaaaacgt ctcgcaaagg ctgaaactgg cagatgtcaa aggcctggga
    12841 taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac cgaatcaaca
    12901 acctcctccc cgcatgatgc ggtatttaaa acctttatat tcacacccga aaccgcacgg
    12961 gattttctcg aaatacattt accagaacca ctgcgcaagc tttgcaacct gcaaacctta
    13021 cgcctggaac ccactagttt tattgaaaaa agtttacgcg cttactactc ggatgttttg
    13081 tggtccgtgg aaaccagcga cggtgacggc tatatctact gcgtgattga acatcaaagc
    13141 tctgcagaaa agaatatggc ttttcggcca atgcgctatg ccactgccgc catgcagagt
    13201 cacctagata aaggctatga cagagttccg ctggtggtgc cattgctgtt ttatcatggc
    13261 gaaacatcgc cctacccgta ctcacttaac tggctggatg agtttgacga tccgcaactt
    13321 gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt acctgacgat
    13381 gagatcatgc aacatcggcg tatagctctg ctggaactga ttcaaaagca tattcgcgac
    13441 cacgatttaa tcggcatggt cgacaggatc accacgcttt tggttagagg cttcactaat
    13501 gacagccagc tacaaacact gtttaattat ctgctgcaat gcggcgatac ctcccgtttc
    13561 acccgtttta ttcaggagat tgccgaacgt tcaccactac aaaaggagag attaatgact
    13621 attgctgaac ggctacggca ggaaggacat caaattggct ggcaagaagg taaattagta
    13681 ggtttgcagc aaggtaaatt agaaggtttg caggaaggta tgcatgaaca agccattaaa
    13741 attgctttgc gcatgctgga acagggcatt gatcgtgacc aagtgctcgc ggccacccag
    13801 ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca cagccgatcc
    13861 ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg cctgatttaa
    13921 acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg ccaacagcga
    13981 caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca gccagaaaac
    14041 cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt cgctgcactt
    14101 ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct ggtccaataa
    14161 acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt gccgttttac
    14221 tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa ttctcaatgc
    14281 gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg tagggagggt
    14341 tggacaaatt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca gagtggcgaa
    14401 atcagacgga taaagtcatg gactcaacaa cctcaatcca gccatgctca ctagcgatgt
    14461 cctggccgtt taaccaacgg cgtagcatat tcagtgccat cattgcgcac acttcctgac
    14521 ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa gtgccgtctg
    14581 gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc agtgccagcc
    14641 cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc agggtttctt
    14701 cctgtgaagg aatcacttca cacgccagca atggagcacc tgcgcgagaa agttgcaaag
    14761 ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg ttttgcaatt
    14821 cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc tgtccggcaa
    14881 cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc ggtccggtga
    14941 gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacgccc ggcggcagtt
    15001 gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta gttaaacgca
    15061 gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg atttcgtgct
    15121 cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg caacgattaa
    15181 gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca gcactggcag
    15241 gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg tggaaatagc
    15301 gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt gctgtcgcag
    15361 cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg atcagcacat
    15421 cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta tcccccaccg
    15481 tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc caggcagcgt
    15541 tagtgtcaac gatttgcccg tataacactt catccccggt ggatagcatt tccactttta
    15601 acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag ataaggcgta
    15661 gtaagaaact gaatgccgcg ccggaaggga tcagaaactg gcgttaaccc caacatacag
    15721 gccgtcagcc acagcgttgt cgcggttacc gtctttaccc gacagattca ggtagcgata
    15781 acccgcctca atactgactg gacgcataat ggtgtaacgc gcgccagcat tcgcttcctc
    15841 ataactttga ataccgctgg agagcgaatc cggagagtag taatactcgc caaacaaacg
    15901 gaagctgttg ccaattttcc actgcaaacc acctcctacc gctgcggcat aaccttcatc
    15961 gccgtaattc gggttggtgt acacgccttt tccgccaacg gtcgccatta acggcccgag
    16021 aggcagattc aaccccagcc ccacgcccgc gacgtcaccg tcgtcgtcgt tatgtgtcca
    16081 gttaccgctt aaagccaggc ccgtcgattc agtcccaaaa ccgacaccaa tattggtgta
    16141 ttctttaccc gcctggccgc tgatgctcat tgcattgacc gatgcagaaa ccaacagcat
    16201 tccggccaga cctgctagcg caattttttt cattatcccc tttcccacaa gcaattaatc
    16261 aaaattccca aagctgcggg attgtactgc ctggcacaga ggattcaatg agcgaaaaaa
    16321 gggatctagt cacttattgt aactaaatac ttcttaaata attaaacgcc gaaaaaataa
    16381 acacacttta ccgatacaca caaaaataat aataagtgca gttatcattg caaaagagat
    16441 tactgtcact ttcacatatt tctgagttac cgtattctca tggtttcttt ttatttataa
    16501 ggaatacccc tgtgcgacaa cggactattg tatgcccttt gattcaaaat gatggtgctt
    16561 atttgctgtg caaaatggcc gacgatcgcg gcgttttccc cggtcaatgg gcgctttcgg
    16621 gtggcggcgt ggagtctggc gaacgaattg aagaggcact acgccgcgaa attcgcgaag
    16681 aactgggaga acagctgctt ttgacagaaa tcacgccgtg gaccttcagc gatgatattc
    16741 gcaccaagac atatgcagat ggtcgcaagg aagagattta tatgatttac ctgatttttg
    16801 actgcgtttc tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac tacgcgtggg
    16861 taaaacctga agatctggtg cattatgatt taaatgttgc cacccgaaaa acgttacgtt
    16921 tgaaaggtct tctttaacaa tgatgacagc catcactggc tgtcagattg aaaatttagt
    16981 ggttaacgaa ttccccatcc agataaactt tgcctttttc cacatgcatg actaaaccat
    17041 ccagataatc aggtttaaat atcgcatcac gcttattttt agcaatatat gtcaggcaat
    17101 gattatgggt gaaaataacg atatttttat caggcgcttt gctttgtaag tccttaattg
    17161 cactataaat ctcattaccg cactgaagaa gtcgtttatc taccgtcagc tttttacccg
    17221 cggaaaacca ggtcgccgac tgaatggtcc ggacggtatt actggaataa agatcgaaat
    17281 cagagatgtc agcgctaaaa gcgttgccca gttcacgggc atcctgggta cctttaaccg
    17341 taatacctgt tttatctgac aagcattgat tggttgaacg atcgcaacgt tcagcatggc
    17401 gaaacaaaac gacaaccggg tgctgctgtg ccagtctggc caatgtttta ttgtcgatac
    17461 gtggcaaacc attgctactc caggcggcat gagtacccag tccggcaatt gcagcgagcg
    17521 ccagtaaaat gatgaaatat ttttttgact tcaatgatga gcggcaaaaa gctaacacgt
    17581 aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc gacatttctg
    17641 gcgataatta gcagttggtg taatattaaa aatcctacga tgtcggcaaa atgcctcaaa
    17701 tttttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata tttacgtcta
    17761 atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc caaatggcaa
    17821 aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg gcggaaggaa
    17881 aagcaatgtc agaatttttg cctttttcgc gaccagcaat gggcgtggag gaactcgctg
    17941 cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat caggcgcttg
    18001 agcaagcctt ttgccagttg acgggaaatc agcatgccat cgcggtcagt tcagccaccg
    18061 ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa gtgattacgc
    18121 cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca acgccggtaa
    18181 tggtggatgt cgaccgcgat acgctgatgg tcacgcctga agcgatcgaa tcagccatta
    18241 cgccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca gatattgacg
    18301 ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct gcccatgccg
    18361 tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt ttttcatttc
    18421 atgccattaa aaatattacc tgtgctgaag gtggcctgat tgtaactgat aatgaaaacc
    18481 ttgcccgcca gctacggatg ctgaaatttc acggtctggg tgtcgatgcc tatgacagac
    18541 aaacctgggg ccgtgcaccg caggctgaag tcttaacacc gggctataag tacaatctga
    18601 ccgatattaa cgccgcgatt gccctgacac agttagtcaa attagagcac ctcaacaccc
    18661 gtcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc tttcagccat
    18721 taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt cgtgtcgatg
    18781 aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa agaggcattg
    18841 gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag cgttttccca
    18901 cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg ccgctgttcc
    18961 cggatatgac taccgccgat gccgaccgcg tcatcacagc ccttcagcaa ctcgcaggac
    19021 aataagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt cccgtttata
    19081 acgagcagga aagcttaccg gaattaatca ggcgcaccac cacagcctgt gaatcgttgg
    19141 ggaaagagta tgagatcctg ctgattgatg acggcagtag cgataattcc gcgcatatgc
    19201 tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc tattttgatt aaccgcaatt
    19261 acgggcaaca ttcagcgatt atggcgggat tcagtcacgt tactggcgac ttaattatta
    19321 cccttgatgc cgatctccag aatccgccag aagaaatccc ccgcctggtg gcaaaagccg
    19381 atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc tggtttcgta
    19441 aaacagcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa gcgatgggtg
    19501 actacggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg ttgcactgcc
    19561 atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt gcaattgaaa
    19621 ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa atacagtttt atgcgcctga
    19681 ttaatttgat gtacgacctg gtgacctgcc ttaccacaac gccgctacgt atgctgagtc
    19741 tgctcggcag cattattgcg attggaggtt ttagcattgc ggtgctgctg gtgattttac
    19801 gcctgacctt cggaccacaa tgggcggcag aaggcgtctt tatgctattt gccgtgctgt
    19861 ttacttttat tggcgctcag tttatcggca tgggattact cggtgaatat atcggcagga
    19921 tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc cgtccatcca
    19981 gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg gatgcctcgg
    20041 tattgaagct ctgctggctg ccggttacga aattagcgcc atttttaccc ataccgataa
    20101 tcccggtgaa aaagcctttt atggttcggt ggctcatctg gcggcggaaa gagacattcc
    20161 ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg cccaactgtc
    20221 accagaggtg attttctctt tttattatcg ccatcttatt tgcgacgaga tttttcagct
    20281 cgctcccgca ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc gtggtcgcgc
    20341 gccgctgaac tgggtgctgg tgaacggtga aacggaaacg ggcgttacat tgcaccgaat
    20401 ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat tgcgccagac
    20461 gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct actggaacag
    20521 acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc
    20581 acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa accggcatcc
    20641 gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt
    20701 ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag
    20761 ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa
    20821 atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagatg
    20881 ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc ccgacgccgt
    20941 acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg
    21001 ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt
    21061 tttctgaatc atccacattt tcactttgtc gaaggcgata tcagtattca ttccgaatgg
    21121 attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat agccacgccg
    21181 attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga gaatctgcgc
    21241 attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt
    21301 tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat cgtcggcccg
    21361 gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg ggtgatctgg
    21421 gcctatggcg aaaaagaggg tttacagttc accctcttcc tcccgtttaa ctggatggga
    21481 ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat tacgcaactc
    21541 attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa acaaaaacgc
    21601 tgctttactg atatccggga cggtatcgag gcgttgtacc acattatcga aaatgcggga
    21661 aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgag
    21721 gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca
    21781 ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact acggcaaagg atatcaggac
    21841 gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg ggagccgaaa
    21901 attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt
    21961 acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatgcc tttcgtggca
    22021 cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca
    22081 tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac
    22141 agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg gatattttac
    22201 tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat atcattcgtg
    22261 aagcggctaa acatcacgaa gtcggtctgc acgcctggga tcaccatgcc tggcaagccc
    22321 gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt cttcgcactc
    22381 tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc
    22441 agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg
    22501 ccataccgtt ccgtccactg cttgaatcag gaaaccctgg cactgcgcaa attccggtga
    22561 ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat tttaacggtt
    22621 ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt catgcagaag
    22681 ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca gctcaggaag
    22741 gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc ggacaagttg
    22801 ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc
    22861 gctgatgaaa tcggtacgtt accttatcgg catcttcgcg tttattgcct gctattacct
    22921 gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg cggaaatcag
    22981 tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc tacgttattt
    23041 cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa
    23101 taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctggtgac
    23161 ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca cagtaattta
    23221 tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc
    23281 attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa
    23341 aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa
    23401 aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa
    23461 acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt
    23521 tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact attttttctg
    23581 ggttgagcat attcaacgct ttgcactaga tgatgcccaa catagagctc cgttctggta
    23641 ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact
    23701 gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat
    23761 aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata ttctttcttg
    23821 ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg ctggcagcaa aaaataatcc
    23881 tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc
    23941 cacatttgtg atctccccct ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag
    24001 ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac
    24061 cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg ggctggcgtt
    24121 gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt
    24181 cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt
    24241 tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg
    24301 tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga
    24361 gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt
    24421 tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca tcgttcgtca
    24481 ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc
    24541 agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata
    24601 aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt
    24661 gccatgatgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg
    24721 ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta
    24781 tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat cctcgggagt
    24841 acggtgtaat gtgcctgata tggggattat tcagcgtcat tattgcctca gttgcgcaat
    24901 taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg gattttatcg
    24961 cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc
    25021 tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc aaagcctatg
    25081 ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg
    25141 aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc
    25201 tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct gtacactgcc
    25261 gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt
    25321 cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg
    25381 cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa
    25441 gtacatgtct gttatcttgt ttattgcagc acgatttttt gtgccagcca ttccattgca
    25501 ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa actctttatc
    25561 aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt gctgacgttg
    25621 cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca gctccggcgg
    25681 taaagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca cccattcgcc
    25741 aagatcaacg gtttgctggt catactccac caccgctacc ggccgatgac caaactcctt
    25801 gtcggcaacg gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa taacacgctc
    25861 gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattgtcta aacgtccgac
    25921 aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc agccttcgtc
    25981 attaaccagt gaaaccagtt gcccgttacg ccagtaacct tctgccatac tggcagcccg
    26041 cagccacact tcattattaa cgattttcac ttcccgaccc ggcagcggca aaccaacgtc
    26101 tgccaggccg tcggcttctt tcgcacatac cgtggaggca aactcggtca gaccatagcc
    26161 gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc
    26221 gccgccaagt aacaccgctt tcagggaaac ggaactgcgg ttaaccagca aacgccagag
    26281 ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atttgctcca atggctgttt
    26341 atcacgcacc gtcatccgcg caccagcgta taaccagcgc cacataattc cctgaccgga
    26401 gacgtgaaac agcggtaaag agagcaacca gtcatcgtga tcgccaaacg gaatcagcga
    26461 taacacacct tgcgcactgg caagatgggc ctgataagta tggacagcgg ctttcggcaa
    26521 accggtagaa cccgaggtca acgtcattga gcacagacgc gtcggctgcc acgcagcggc
    26581 atgtacgcct tcaaccagct gaatgtgcag cgacgttaat gccggaaacg tgttttcccc
    26641 atccggcacc agcgcaaatt gcagcgtcag attgggcagc aattcttcaa gcaacggttg
    26701 cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg ccagccaggc
    26761 gagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct caaccacccc
    26821 ctgcgccgca aagcccgtag ctaactcatc gacgcgagca caaagctcgc gccagttgag
    26881 ttgctcgtca ttaagacgta aggcgatggc ttctccccgc acttgccgcc agtgacgcca
    26941 cggccagtca gagaagatca taacaactgc tcctgtacat cagcgtccac taacggcagt
    27001 gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt gtccagtccc
    27061 ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag acctaagctc
    27121 gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc ctgtacctgc
    27181 tcgcgtactt tttccagact gcccgtgagc gtgggtttga taactaccgc gcgcacgccc
    27241 tcttcagcca caaaggcaaa atccggctcg cgcaggcttt catcccaggc aatggcaatg
    27301 ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc ttcgagaaac
    27361 gcgatgcggt ggcgataatc cgggttaaca tatttggcaa actgctgacc tttcagcggt
    27421 gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc cagcaacaga
    27481 ttcaccacca tgccgtcgcg caccgcttcg tacaatccca ctttgacctt cgccactttc
    27541 tcgcccggca tatcggcaag tttgaggatc agatcgtccg gatcgccatt acacagcggt
    27601 gccgcacggt agttggctgc ttgcggcaac gtatctgtca gttctgccaa tgcacagctt
    27661 acgccaaagg ccacggaagg catctgcggt agctcgcaat cgcctgccag ccagttattt
    27721 acccaggcaa gcagcacgct ttgcgcctct tcccagtttt cctggctgaa gcccggcagt
    27781 ggggagctct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta cagcccgtcg
    27841 cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat ctgccagcgg
    27901 tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca ggctgacgtt
    27961 tctggttgaa agcgttgcga ccttcctgac cttcttccgt catgtagaac agcatggtgg
    28021 cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc agcgcagctt
    28081 tcaggcagcg cagcgccatc gggctgtttt gcagcatttc gcggcaccaa cggacggttt
    28141 ctttttccag atccgccagc ggtaccacgg tgttgacaag gcccatatcc agcgcctgtt
    28201 ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc ttttttctgc ccgacgatac
    28261 gagccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg ccagtctgac
    28321 cgaagatggc attatctgct gcgatagtca ggtcgcacat catgtgcaga acgtgaccac
    28381 cgccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta cggatctgac
    28441 gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag ccgccgtaat
    28501 caccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg ccagtcagaa
    28561 tgatcacgcc gatattgtcg tcataacgcg catctgccag tgcctggatc atctctttta
    28621 ccgtcagagg acggaaggca ttgcgcacct gcggacgatt aatggtgatt tttgcgatac
    28681 cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg tgccattcaa
    28741 ccggtgcgta aggcattgct tcatcaggat aaatcatatt gtgtccttta gtcagaaacg
    28801 caagatctgc gccagacttg cgattacgcc agcgggattt tcccgatgcg cgttatgtcc
    28861 ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagggcgc ggaatttgct
    28921 gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa
    28981 atcaggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg cgccattatt
    29041 gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg caaaaacagg
    29101 ctgttgatac cagtcggcaa ataccgccgt taacggttct gtgcgaaaac gctgcgccca
    29161 ttggcgatcg gaacgctgac gttccgcacg ttgttcagca ttttgcagcc ccggatgccc
    29221 gccttcgaca ataaccccac aaagccccgc cagcccctgg caagccgcca tcatcgccac
    29281 ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg atgttgtaac taaccaaggt
    29341 tttacgcagt aagtcggtga catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc
    29401 atgacctagg agatcaacat acaaccgtga gtagtcggca aacgcctcgc ccacttcttg
    29461 ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc agccagggta aacctagttt
    29521 tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag aagttgctgg
    29581 agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt ggttggcgtg
    29641 cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt tttgcggatg atgatatttc
    29701 agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc
    29761 tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt gttgttcacc
    29821 acaattaata ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt gagatcgtaa
    29881 agcgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg ctgaacgcct
    29941 gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc ggttgctgta caccgggtaa
    30001 cctgccggaa gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt accaacaaac
    30061 aattgcccct gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac
    30121 gcatcacggc gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg gatttcaacg
    30181 caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat attggcaatt
    30241 aagcgacgtc cgcgatggtg tgccggatca agtcgccctt caatgtcatc aacaatccag
    30301 tactcttctg gttcacagct tgcctgccat tgcaggagcc gtttccccgt caggctactt
    30361 cccagttgca ccacaatttg cgcctgctgc agctcgctgg tcgctttggc attgcctaac
    30421 caaagatcgg cacacggcag cggctgcccg gtttgtgaca gcacatcgcc aatcagcggc
    30481 cagccaagag tttgcgccca cagggcaact tttttgccct cttccgcact catgcgcccg
    30541 gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt ttcactttcc
    30601 agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcacc cagacgctgt
    30661 tgccagctaa gcccggtatc gtccatttcg ccatacagcg gttcagcaaa cgggcagttg
    30721 atatggactc cccccgcatg aagcgtaccg agagcgtggt cgatggtaga aaccagccaa
    30781 cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg gtgagaggcg
    30841 aacattcccg gctggcgaat tgcctgattc gcgccgcagt caattagctc cggcgggcga
    30901 tcggcggtta agagaatcag tttttctccg gttaacccgg cttcaatcag tgccggatag
    30961 agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt gctgactttc
    31021 gccagcccca gcgccagatg ccccaaccca cgctcatcga aatgggtgtg gtgaatgaat
    31081 gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg ggcgatacag
    31141 atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca gcgtcggtta
    31201 aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta taaggagcta
    31261 caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt ccatttgtaa
    31321 taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct cggggtcgga
    31381 accacggaca atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa tttttgctga
    31441 gcgcagggaa acgcagaatt cgctttgttg taatgagaga tagcccgcag aaccggcgta
    31501 ccattctcgg gtgaacggtt cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa
    31561 gccagcaact gctgccgttg gcagcaactg atgtaaacag atcacatcat ccgctttgtt
    31621 gagtgaagtc cagatacagc ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg
    31681 cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt tggcagatat cttccaccac
    31741 cagcatgttc tcgcgctggt ttttatcatc cgccatcagc cactctccta actgctgcgc
    31801 ctgcttatca tcaggatgat ttgctactgt tcccgccagc gcttcagtac gcagcgcttt
    31861 gtcacgccgc cgccataacc gttccggtga agagccaaga aaagcatttt cgccatcaaa
    31921 ggccatgtaa aaatggtagc aattcagatt cagtcgacga ctggcagcca tcatcgccgc
    31981 cgcgttgacc ggacttgcga aatgcaggtc agttgcccga gcgagcacca ctttgtcgag
    32041 ctcaccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc
    32101 cggccagtgt tgttctcgcg tggtggttaa atgtaaccca ggcaagggct tgatactcac
    32161 cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc tttcgctgaa
    32221 tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc ggggtaaaag
    32281 taaattgccc tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt cagcgtgttc
    32341 cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc
    32401 caggacggca gcttcttcat caccattacg ttgttgccag tagaattgcg ggtacgtttg
    32461 ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag gaatatcgat
    32521 aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta gattttccag
    32581 cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca ttatacgggg
    32641 tactacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg ccagcaacag
    32701 tcctagtacc agcccgacgg ccgcgcccac accaattcct tgccagggtt tttcgtggac
    32761 gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt ctgaagcctg
    32821 gctaacccgt tttttcacat catccagcgc tttttctgca tgcgctttca gctcaacata
    32881 tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt cactaagcag
    32941 cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta gacattctcg ttctccattt
    33001 gcgtaaaacc tgtcactaac tatagacaag agttgttgat tacgcctgaa ttacctcgcg
    33061 tgccatccca atgtgtggga taccatcttc ttcatagacc tccgtcacag ggataaaacc
    33121 gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag gcttatcagg
    33181 ccagtgatgc gtacatgttt cgagtgtttt actcatcagc tgctgcccca ctttttcacc
    33241 acgcaacgct tcactgacaa tcacccgacc tataacgacc ggctcaagat catcatcact
    33301 tttcagaatc ctcgcatacg ccaccagttc atcatttttc caaccgagaa tatggcgatt
    33361 atcccccgtc aaatcatcac catcgatatc ctgataaggg caattctgct cgacaacaaa
    33421 caccgcgcaa cgcagttgta ataaggcata aagctgggaa accgataatt cagagtgatg
    33481 cagatcttgc cattcaatca taatgctctc cttttagatg tatgccttat actagccgga
    33541 atattttttg aaacgtgatg aactcatgga attaattttt ttaggtactt cagccggtgt
    33601 gccaacccgc acgcgcaatg tcacggcaat attgcttaac ctgcaacatc cgacccagag
    33661 cggactttgg ttgtttgact gcggtgaagg cacccagcat cagctactgc ataccgcctt
    33721 taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc atctttttgg
    33781 tttacccggc ttgctgtgca gtcgttctat gtcaggcatt atccaaccct taacgattta
    33841 tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg cggattagcg gctcatggac
    33901 cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attctcgatg atggcctgcg
    33961 caaagtaacc gcttatccgc tggaacaccc actggaatgt tatggctatc gtattgaaga
    34021 acatgataaa ccgggtgcat taaatgccca ggcattaaaa gctgctggcg tgccgcctgg
    34081 cccactgttt caggaattaa aagcgggcaa aacaatcacg ctggaagatg gaaggcagat
    34141 taacggcgca gattacttag ctgctccagt gccaggtaaa gcgctcgcta ttttcggcga
    34201 taccggcccc tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca tggtgcacga
    34261 agcgacgctg gatataacca tggaagccaa agccaatagt cgcggccata gctctacacg
    34321 ccaggctgcg acactagccc gtgaggctgg agtcggcaag ctaatcatta cccacgtcag
    34381 ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt caattttccc
    34441 ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt taactctgct tattattaaa
    34501 aacagggtga aacttgccct gttatagcaa cccgcgctac atatatccac ccacacaggc
    34561 atattgagcg atttttgata tttattcatc tccttacgca ctatattcag agcaataagt
    34621 tgcctctttt catcttgatt cagcacaacc tttgcaacta aaaggagccc atgatggtta
    34681 cagttgtcag caattattgt caattatctc aaacccaact cagtcagaca tttgcagaaa
    34741 aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta tccggagatg
    34801 aagaaagtat tgagttactg cataatattg cgttaggtta tgatgaattt ggaaaaaaag
    34861 ctgggtaatg ctgccaactt actgatttag tg
//
LOCUS       DAJTRM010000032        34851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRM010000032 DAJTRM010000000
VERSION     DAJTRM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.032"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(18..93)
                     /locus_tag="OO322_001694"
     tRNA            complement(18..93)
                     /locus_tag="OO322_001694"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="OO322_001695"
     tRNA            complement(152..228)
                     /locus_tag="OO322_001695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="OO322_001696"
     CDS             complement(331..1716)
                     /locus_tag="OO322_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5906613.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO322_001697"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO322_001697"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5906614.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO322_001698"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO322_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5906615.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO322_001699"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO322_001699"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5906616.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO322_001700"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO322_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5906617.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO322_001701"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO322_001701"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5906618.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO322_001702"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO322_001702"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5906619.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO322_001703"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO322_001703"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5906620.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO322_001704"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO322_001704"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5906621.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO322_001705"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO322_001705"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5906622.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO322_001706"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO322_001706"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5906623.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO322_001707"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO322_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5906624.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO322_001708"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO322_001708"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5906625.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO322_001709"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO322_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5906626.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO322_001710"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO322_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5906627.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO322_001711"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO322_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5906628.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="OO322_001712"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="OO322_001712"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5906629.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="OO322_001713"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="OO322_001713"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5906630.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO322_001714"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO322_001714"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5906631.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="OO322_001715"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="OO322_001715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5906632.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO322_001716"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO322_001716"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5906633.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="OO322_001717"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="OO322_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5906634.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO322_001718"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO322_001718"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5906635.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO322_001719"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO322_001719"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5906636.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO322_001720"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO322_001720"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5906637.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO322_001721"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO322_001721"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5906638.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="OO322_001722"
     CDS             complement(29521..31167)
                     /locus_tag="OO322_001722"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5906639.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="OO322_001723"
     CDS             complement(31170..31220)
                     /locus_tag="OO322_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906640.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO322_001724"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO322_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5906641.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33235
                     /locus_tag="OO322_001725"
                     /pseudo
     CDS             31756..33235
                     /locus_tag="OO322_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO322_001726"
     CDS             complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO322_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5906642.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33717..34556
                     /gene="hdfR"
                     /locus_tag="OO322_001727"
     CDS             33717..34556
                     /gene="hdfR"
                     /locus_tag="OO322_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5906643.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(34652..34727)
                     /locus_tag="OO322_001728"
     tRNA            complement(34652..34727)
                     /locus_tag="OO322_001728"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34692..34694),aa:Trp,seq:cca)
     gene            complement(34736..34812)
                     /locus_tag="OO322_001729"
     tRNA            complement(34736..34812)
                     /locus_tag="OO322_001729"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34776..34778),aa:Asp,seq:gtc)
BASE COUNT         8500 a         9537 c         8801 g         8013 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc
    32881 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    32941 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    33001 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga
    33061 aacgctgatc catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg
    33121 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    33181 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    33241 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    33301 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    33361 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    33421 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    33481 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    33541 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    33601 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    33661 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    33721 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    33781 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    33841 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    33901 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    33961 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
    34021 gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac
    34081 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    34141 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    34201 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
    34261 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    34321 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    34381 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    34441 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    34501 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgatgtg tattaatctg
    34561 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa
    34621 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    34681 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    34741 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    34801 aactaccgct ccaccgaatt cttttacaac caccggtttt acgaccggct t
//
LOCUS       DAJTRM010000033        34666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRM010000033 DAJTRM010000000
VERSION     DAJTRM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.033"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..805)
                     /locus_tag="OO322_001730"
     CDS             complement(<1..805)
                     /locus_tag="OO322_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaH family type III secretion system E3
                     ubiquitin--protein ligase"
                     /protein_id="HCR5906644.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="OO322_001731"
                     /pseudo
     CDS             897..983
                     /locus_tag="OO322_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1388..1864)
                     /locus_tag="OO322_001732"
     CDS             complement(1388..1864)
                     /locus_tag="OO322_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5906645.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="OO322_001733"
     CDS             complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="OO322_001733"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5906646.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3299..4339
                     /gene="bioB"
                     /locus_tag="OO322_001734"
     CDS             3299..4339
                     /gene="bioB"
                     /locus_tag="OO322_001734"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5906647.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4336..5490
                     /gene="bioF"
                     /locus_tag="OO322_001735"
     CDS             4336..5490
                     /gene="bioF"
                     /locus_tag="OO322_001735"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5906648.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5477..6232
                     /gene="bioC"
                     /locus_tag="OO322_001736"
     CDS             5477..6232
                     /gene="bioC"
                     /locus_tag="OO322_001736"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5906649.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6225..6902
                     /gene="bioD"
                     /locus_tag="OO322_001737"
     CDS             6225..6902
                     /gene="bioD"
                     /locus_tag="OO322_001737"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5906650.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="OO322_001738"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="OO322_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5906651.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="OO322_001739"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="OO322_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5906652.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="OO322_001740"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="OO322_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5906653.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="OO322_001741"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="OO322_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5906654.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="OO322_001742"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="OO322_001742"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5906655.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="OO322_001743"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="OO322_001743"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5906656.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="OO322_001744"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="OO322_001744"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5906657.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13839..14543
                     /locus_tag="OO322_001745"
     CDS             13839..14543
                     /locus_tag="OO322_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5906658.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14748..15461
                     /locus_tag="OO322_001746"
     CDS             14748..15461
                     /locus_tag="OO322_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5906659.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15497..16453)
                     /locus_tag="OO322_001747"
     CDS             complement(15497..16453)
                     /locus_tag="OO322_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5906660.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="OO322_001748"
     CDS             complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="OO322_001748"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5906661.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17691..18452)
                     /locus_tag="OO322_001749"
     CDS             complement(17691..18452)
                     /locus_tag="OO322_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5906662.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18585..18995
                     /locus_tag="OO322_001750"
     CDS             18585..18995
                     /locus_tag="OO322_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5906663.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18957..20063)
                     /locus_tag="OO322_001751"
     CDS             complement(18957..20063)
                     /locus_tag="OO322_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5906664.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20074..21207)
                     /locus_tag="OO322_001752"
     CDS             complement(20074..21207)
                     /locus_tag="OO322_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5906665.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21200..22936)
                     /locus_tag="OO322_001753"
     CDS             complement(21200..22936)
                     /locus_tag="OO322_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5906666.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="OO322_001754"
     CDS             complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="OO322_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5906667.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="OO322_001755"
     CDS             complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="OO322_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5906668.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24827..26014
                     /gene="rhlE"
                     /locus_tag="OO322_001756"
                     /pseudo
     CDS             24827..26014
                     /gene="rhlE"
                     /locus_tag="OO322_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="OO322_001757"
     CDS             complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="OO322_001757"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5906669.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26823..28973
                     /gene="dinG"
                     /locus_tag="OO322_001758"
     CDS             26823..28973
                     /gene="dinG"
                     /locus_tag="OO322_001758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5906670.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29001..29963
                     /gene="ybiB"
                     /locus_tag="OO322_001759"
     CDS             29001..29963
                     /gene="ybiB"
                     /locus_tag="OO322_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5906671.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30104..31189
                     /gene="hcxB"
                     /locus_tag="OO322_001760"
     CDS             30104..31189
                     /gene="hcxB"
                     /locus_tag="OO322_001760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5906672.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31418..31678)
                     /locus_tag="OO322_001761"
     CDS             complement(31418..31678)
                     /locus_tag="OO322_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5906673.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31943..32209)
                     /gene="ybiI"
                     /locus_tag="OO322_001762"
     CDS             complement(31943..32209)
                     /gene="ybiI"
                     /locus_tag="OO322_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5906674.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32283..32960)
                     /gene="ybiX"
                     /locus_tag="OO322_001763"
     CDS             complement(32283..32960)
                     /gene="ybiX"
                     /locus_tag="OO322_001763"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5906675.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33002..34666)
                     /locus_tag="OO322_001764"
                     /pseudo
     CDS             complement(33002..34666)
                     /locus_tag="OO322_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8220 a         8877 c         9361 g         8208 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg ggaaggtaaa
     1321 aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc
     1381 agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt
     1441 taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct acatccagcg
     1501 cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc
     1561 cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag
     1621 agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact acccagtgcc
     1681 accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc
     1741 ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc
     1801 cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt
     1861 tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gctgacttcc
     1921 cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc
     1981 tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc
     2041 cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt caccggacga
     2101 gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat ttcggcatca
     2161 cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg ctgctgccat
     2221 tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag cggattgccc
     2281 ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttctgcaacc
     2341 tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg
     2401 cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc ggtacgacca
     2461 aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg
     2521 attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggc
     2581 tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc aaaaccaacc
     2641 atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt
     2701 tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc
     2761 gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg
     2821 cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc tacggaaccg
     2881 gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag tttgcggcat
     2941 agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat ggcatcaatt
     3001 tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc cgcccaccag
     3061 gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca accttcggcg
     3121 ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg ccagatatgg
     3181 cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga
     3241 tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga gaagccccat
     3301 ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct
     3361 ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc gtcaggtgca
     3421 ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca aatactgccc
     3481 gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag ttgaacaggt
     3541 gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta tgggcgcggc
     3601 atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc agggggtaaa
     3661 agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc aggcgcagcg
     3721 cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc cggagtttta
     3781 cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg aaaaagtgcg
     3841 cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa cggtaaaaga
     3901 tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa gcgtaccaat
     3961 caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg ttgatgcctt
     4021 tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt acgtgcgcct
     4081 ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggcaggcgc
     4141 aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag aagataaaga
     4201 cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc tggcagggga
     4261 taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg acgaatatta
     4321 caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt
     4381 gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg ctggctggtg
     4441 gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat
     4501 ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg tagcggcggc
     4561 tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga actggccgag
     4621 tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa tcaggcagtc
     4681 attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag ccatgcctca
     4741 ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca taacgatgtc
     4801 gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt ggtgacagaa
     4861 ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca ggtaacgcaa
     4921 cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat cggggagcag
     4981 gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt gacttttggc
     5041 aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc ggattatctg
     5101 ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca ggcgcaggca
     5161 ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg cgaaaaactg
     5221 gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac gctggctgat
     5281 tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca
     5341 gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac cgtacccgct
     5401 ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga tatcgaccgt
     5461 ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg catttggtcg
     5521 ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg acgccttact
     5581 ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg gacctggctg
     5641 gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc tctcgccgcc
     5701 aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag gagatatcga
     5761 atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg cggtgcagtg
     5821 gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt
     5881 ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgcggcaggc
     5941 ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg aagagtcgct
     6001 gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg atgatgcgct
     6061 cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag ggcgcgaccc
     6121 gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc aacaacaggg
     6181 gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt aaacgttatt
     6241 ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca cttttacaag
     6301 ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct ggcagcgaaa
     6361 agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac agcagcctgc
     6421 agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg ccgcacatca
     6481 tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga ttacgcgcgc
     6541 ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt acgccgcttt
     6601 ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg atactggtag
     6661 ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca atacaacacg
     6721 ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga aaacgtcacg
     6781 ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga gagatcccct
     6841 ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tgtccgccac
     7861 caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg aatatctcga
     8881 agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg agcgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca acttactgcg
     9001 tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agttgcgtga
     9481 gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa cggaaaacag
     9541 tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa tttgcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcaacagg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgtt
    10381 cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc atttgaggcc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt gattacgggt
    12241 ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt gttcgaccgt
    12301 gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt attcaggcgg
    12721 caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggcgga
    13321 gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc ataacctggg
    13381 cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatct
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg cagatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc ttcgcttggc
    15541 tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa tacaggacgc
    15601 gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca gccagtagtg
    15661 cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg acgccagcaa
    15721 tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga ccaagtaaca
    15781 gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc tgagcgaggg
    15841 cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg cggtgcttcg
    15901 cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg ccgagaatac
    15961 gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct gcggtaaaga
    16021 taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc gaggaaatcc
    16081 gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc ataccaatgc
    16141 cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac accagcatca
    16201 cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt
    16261 aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg cgattgtagt
    16321 cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc accagcaaca
    16381 caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc cagcgcgggt
    16441 gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat cctgcgtttc
    16501 cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg ccggaagcca
    16561 gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt
    16621 ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg cggcaataat
    16681 gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga tgacatttgc
    16741 ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg tcgcccagtg
    16801 atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa aaacctgaac
    16861 gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa tcggcatatc
    16921 cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc
    16981 gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt cccgctgcgc
    17041 ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt cgttatcgcg
    17101 ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt tgtgatgacg
    17161 tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa actggagaat
    17221 atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta
    17281 gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac gcgcgtcgat
    17341 caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa aaaggcgagg
    17401 gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga caaactcatc
    17461 gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc cgcgttgcgc
    17521 tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc agataaacgt
    17581 ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg gataatattg
    17641 ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt tcataaatga
    17701 atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg
    17761 gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag tagaggaaat
    17821 tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc atccagtccg
    17881 gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc acccgccacc
    17941 aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc aagctgcgcc
    18001 tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc ttttccggtc
    18061 atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca
    18121 tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc gtgatgccct
    18181 tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc ggcgagaaac
    18241 tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc
    18301 atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc gcgaagttcc
    18361 ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat ggtgagcacc
    18421 ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc tcttatctca
    18481 ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt
    18541 cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag tggcaacaac
    18601 gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta ctggtagtgg
    18661 cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg ggattatgcg
    18721 cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc gagcaacgct
    18781 ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc
    18841 tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg gcaattgcag
    18901 ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat aagaagttac
    18961 atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt
    19021 agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata aatctgcttg
    19081 gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag ccacactggc
    19141 atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac
    19201 acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag accgaatccc
    19261 accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga tccggcgaag
    19321 gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa ggtggcgaca
    19381 attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg cgaaaccagt
    19441 agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt
    19501 acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt gtagtcgaga
    19561 ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg ttttccttcc
    19621 agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc aatttgcgca
    19681 ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa ggtatccagt
    19741 ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt gtcgatggtc
    19801 gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct ggcgcgggca
    19861 aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat ggcgatggtg
    19921 gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat tagcacgggt
    19981 aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc tttgcggatt
    20041 aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt tggttttcag
    20101 ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt ttaccaccag
    20161 caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga aataacgagc
    20221 gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa
    20281 gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag cggcattgaa
    20341 ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga gtaaaaacag
    20401 gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga gaataaacac
    20461 tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag ggatcagctt
    20521 acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca tggtgccgcg
    20581 ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat
    20641 ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa accagtagcg
    20701 ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg
    20761 ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat tcggctcact
    20821 gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct gttcggcgaa
    20881 atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg cgatcagttc
    20941 ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa
    21001 atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct tgctggagtc
    21061 gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta ccgcaatcag
    21121 ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg cccgcacgcg
    21181 atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg tggatcaact
    21241 gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac tgtgctttca
    21301 aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg atgcggtcgc
    21361 aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct ttctctacca
    21421 tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg gaagtcggtt
    21481 cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
    21541 taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg ccaaatgcct
    21601 cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
    21661 aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt tgcgccatat
    21721 agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc agcaccagcg
    21781 cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttacccg
    21841 cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat
    21901 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
    21961 cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag gttccggcac
    22021 cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg atgttgattt
    22081 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg aggatcagac
    22141 gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag gcgcgttgca
    22201 acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg gccatggttt
    22261 gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc agtaacacgt
    22321 cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac atcccttcgc
    22381 ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca acgccgacgc
    22441 cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc aggccgagtt
    22501 tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg
    22561 taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat
    22621 ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca aatttctgcg
    22681 gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca aagccaatca
    22741 ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc agtgtggttt
    22801 tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg gtacaatcga
    22861 gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga
    22921 taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg cattccctgg
    22981 cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag gtcggtacgc
    23041 agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac gaaaccaatc
    23101 tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt gcgccccggc
    23161 tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg acgcgttagt
    23221 gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac cgcgcgcgtt
    23281 aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag ctccgcctgc
    23341 gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc ctgttcacgg
    23401 ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc ctgtgcctgg
    23461 tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct tttccacagt
    23521 ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc
    23581 gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag atcatactgc
    23641 gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata cggcttgtga
    23701 tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac cgccagcgat
    23761 tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac gttgccatac
    23821 agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc aaccacggcg
    23881 gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac aaactccttt
    23941 gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg atcagttcgg
    24001 tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct ttgccaagac
    24061 ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg gtgtcattag
    24121 cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca
    24181 cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg gagataaact
    24241 tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg caggcgcgaa
    24301 ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag cgttcggctt
    24361 cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg gcgcaggcga
    24421 ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc tgcccggcct
    24481 gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc gccagtgcgg
    24541 cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga ttattcatcg
    24601 cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg gagttgtcca
    24661 gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct gcgtcacaaa
    24721 aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct tttcagtatc
    24781 tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt ctttcgattc
    24841 tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc gtgaacccac
    24901 ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga tggctagcgc
    24961 ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac acctgatcac
    25021 tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg
    25081 tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg
    25141 ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt tgcgcggcgg
    25201 cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc aaaatgcagt
    25261 gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg
    25321 ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc agaacctgct
    25381 gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt tgcacaaccc
    25441 gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc agcacgttca
    25501 ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta aagggaactg
    25561 gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg ctgagcagct
    25621 caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag gtgcgcgtac
    25681 ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga ccgatatcgc
    25741 tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac tgccaaacgt
    25801 accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc
    25861 gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa aactgctgaa
    25921 aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa tcaaagccga
    25981 accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca aacgcaaaac
    26041 tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg
    26101 cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc
    26161 agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg
    26221 ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca gtcgattctt
    26281 gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt
    26341 tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga atttagcgcg
    26401 aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa gtggcttgga
    26461 acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga tctcttcgcg
    26521 gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg gccaggtatt
    26581 cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt catcgctggt
    26641 gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg
    26701 cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat
    26761 tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc
    26821 tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg
    26881 aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca
    26941 aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc ggcgttggga
    27001 aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg
    27061 tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat ttaccgctgc
    27121 tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg cgctacgttt
    27181 gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat ctgctggcgt
    27241 ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt gcgaagctga
    27301 agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat atcgctatag
    27361 atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt
    27421 actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa gcggaagtgg
    27481 tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc
    27541 cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg
    27601 cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg gacttgttca
    27661 cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga
    27721 tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca
    27781 acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg
    27841 aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc
    27901 gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc cacgatattg
    27961 tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt gaggtgcaaa
    28021 gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg
    28081 cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg ggaatacgtg
    28141 tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg
    28201 caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt ctgaaagaga
    28261 aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa
    28321 ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa cacattgccg
    28381 aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt atgttggtac
    28441 tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgcctga
    28501 tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac cgcaaacgcg
    28561 ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa gggcttgatt
    28621 tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca
    28681 gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg
    28741 tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa
    28801 gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca aaaaattatg
    28861 gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag
    28921 gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg
    28981 agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga
    29041 aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct catatgctca
    29101 atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt atcaaagggg
    29161 aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga
    29221 cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac
    29281 aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt cctgtggtgg
    29341 ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc gaactgatgg
    29401 gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg
    29461 tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc
    29521 ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag
    29581 gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc gtcgcgaagt
    29641 tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc gaagtgtatg
    29701 ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt
    29761 atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca
    29821 cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa
    29881 tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc gatggcctgg
    29941 cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt catccggctt
    30001 ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc ccggcgtgca
    30061 gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg
    30121 ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga
    30181 ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg cagggcatga
    30241 ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca
    30301 aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga
    30361 tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg agaaagcgca
    30421 tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc gtatcggtta
    30481 ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg
    30541 tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atcccttctg
    30601 tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat
    30661 tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct
    30721 gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg
    30781 gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct
    30841 tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga
    30901 tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg gcgcgccgga
    30961 ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc atgatgacga
    31021 taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca
    31081 ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc ggcagattgg
    31141 tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg
    31201 tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg
    31261 tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgattcgt
    31321 ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt
    31381 gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac
    31441 cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg
    31501 ccgctttctc agccagtttc gcttccaggg catcgagggg ggatgcgcca tcggcagaaa
    31561 ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc gccgcgaaca
    31621 cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa
    31681 ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc
    31741 acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa ataaattgat
    31801 tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg ttttatgccg
    31861 cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa attatactcg
    31921 ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct ctgcgattat
    31981 atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata cataagcgca
    32041 cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct tcacattcat
    32101 acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct tcaattgtac
    32161 tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc
    32221 catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg gcagttcaag
    32281 cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag cgacaggatc
    32341 tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag ttcaaacagc
    32401 atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga tgccactcgt
    32461 acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa caccagatca
    32521 cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag ttcgccgccg
    32581 tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg catccagccg
    32641 ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc attgttctga
    32701 tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca
    32761 tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgt gctgcgggtg
    32821 tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc
    32881 acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg
    32941 ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc ccacatctga
    33001 atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg tgataacggt
    33061 agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa acgtttagct
    33121 ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc cagtaacctt
    33181 ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg ccgatatagc
    33241 gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat aaggtgaagg
    33301 cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct ttgccgtttt
    33361 tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga gtgatattcc
    33421 ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag taagtcccgt
    33481 catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca
    33541 ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct ttttgcggtt
    33601 taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg aagttgttgc
    33661 cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt tccgtcaggt
    33721 ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg tcgacggtgg
    33781 tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc
    33841 cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc ccgccgttca
    33901 gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct
    33961 gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat
    34021 gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc tgcgtttcac
    34081 gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag aacgtcgagg
    34141 tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc gccgtgcgtg
    34201 actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc ccgcccataa
    34261 tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg
    34321 tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt
    34381 aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga ttcagggccg
    34441 ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg
    34501 gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct gtaccaaggc
    34561 caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg cgtccggcat
    34621 cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatc
//
LOCUS       DAJTRM010000034        34466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRM010000034 DAJTRM010000000
VERSION     DAJTRM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.034"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..332)
                     /locus_tag="OO322_001765"
     CDS             complement(<1..332)
                     /locus_tag="OO322_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906676.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO322_001766"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO322_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5906677.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO322_001767"
     CDS             complement(1693..2073)
                     /locus_tag="OO322_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5906678.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO322_001768"
     CDS             complement(2087..2503)
                     /locus_tag="OO322_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906679.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO322_001769"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO322_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5906680.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO322_001770"
     CDS             complement(3504..3971)
                     /locus_tag="OO322_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5906681.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO322_001771"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO322_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5906682.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO322_001772"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO322_001772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5906683.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO322_001773"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO322_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5906684.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO322_001774"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO322_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5906685.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO322_001775"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO322_001775"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5906686.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO322_001776"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO322_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5906687.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO322_001777"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO322_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5906688.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO322_001778"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO322_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5906689.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO322_001779"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO322_001779"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5906690.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO322_001780"
     CDS             complement(14626..15441)
                     /locus_tag="OO322_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5906691.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO322_001781"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO322_001781"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5906692.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO322_001782"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO322_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5906693.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO322_001783"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO322_001783"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5906694.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO322_001784"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO322_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5906695.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO322_001785"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO322_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5906696.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO322_001786"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO322_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5906697.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO322_001787"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO322_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5906698.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22805
                     /gene="tatC"
                     /locus_tag="OO322_001788"
     CDS             22008..22805
                     /gene="tatC"
                     /locus_tag="OO322_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5906699.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKAEAESEKTEE"
     gene            22847..23629
                     /gene="tatD"
                     /locus_tag="OO322_001789"
     CDS             22847..23629
                     /gene="tatD"
                     /locus_tag="OO322_001789"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5906700.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23626..24114)
                     /gene="rfaH"
                     /locus_tag="OO322_001790"
     CDS             complement(23626..24114)
                     /gene="rfaH"
                     /locus_tag="OO322_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5906701.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24281..25774
                     /gene="ubiD"
                     /locus_tag="OO322_001791"
     CDS             24281..25774
                     /gene="ubiD"
                     /locus_tag="OO322_001791"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5906702.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25820..26521
                     /gene="fre"
                     /locus_tag="OO322_001792"
     CDS             25820..26521
                     /gene="fre"
                     /locus_tag="OO322_001792"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5906703.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26713..27876)
                     /gene="fadA"
                     /locus_tag="OO322_001793"
     CDS             complement(26713..27876)
                     /gene="fadA"
                     /locus_tag="OO322_001793"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5906704.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27886..30075)
                     /gene="fadB"
                     /locus_tag="OO322_001794"
     CDS             complement(27886..30075)
                     /gene="fadB"
                     /locus_tag="OO322_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5906705.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30265..31596
                     /gene="pepQ"
                     /locus_tag="OO322_001795"
     CDS             30265..31596
                     /gene="pepQ"
                     /locus_tag="OO322_001795"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5906706.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31596..32210
                     /locus_tag="OO322_001796"
     CDS             31596..32210
                     /locus_tag="OO322_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5906707.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32249..33700
                     /gene="trkH"
                     /locus_tag="OO322_001797"
     CDS             32249..33700
                     /gene="trkH"
                     /locus_tag="OO322_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5906708.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33712..34257
                     /gene="hemG"
                     /locus_tag="OO322_001798"
     CDS             33712..34257
                     /gene="hemG"
                     /locus_tag="OO322_001798"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5906709.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8119 a         8701 c         9308 g         8338 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ctctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aagctgaagc agaaagcgaa aaaactgaag
    22801 aataaattca accgcccgtc agggcggttg tcatatggag tacaggatgt ttgatatcgg
    22861 cgttaatttg accagttcgc aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt
    22921 tgacgcggga gttaatgggc tactcatcac cggcactaac ctgcgtgaaa gccagcaggc
    22981 gcaaaagctg gcgcgtcagt attcgtcctg ttggtcaacg gcgggcgtac atcctcacga
    23041 cagcagccag tggcaagctg cgactgaaga agcgattatt gagctggccg cgcagccaga
    23101 agtggtggcg attggtgaat gtggtctcga ctttaaccgt aacttttcga cgccggaaga
    23161 gcaggaacgc gcttttgttg ctcagctacg cattgccgca gaattaaaca tgccggtatt
    23221 tatgcactgt cgcgatgccc acgagcggtt tatgacattg ctggagccgt ggctggataa
    23281 actgcctggt gcggttcttc attgctttac cggcacacgc gaagagatgc aggcgtgcgt
    23341 ggcgcgtgga atttatatcg gcattaccgg ttgggtttgc gatgaacgac gcgggctgga
    23401 gctgcgggaa ttgttgccgt tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc
    23461 gtatctgctc ccgcgcgatc tcacgccaaa gccatcatcc cggcgcaacg agccagccca
    23521 tctgccccat attttgcaac gtattgcgca ctggcgtgga gaagatgccg catggctggc
    23581 tgccaccacg gatgccaatg tcaaaacact gtttgggatt gcgttttaga gtttgcggaa
    23641 ctcggtgttc ttcacactgt gcttaatctc tttattaata agattaagca atagcatgga
    23701 gcgagcctca ccatcgggtt cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt
    23761 aataatcacc ttatcacccg gataaggggg ggccggatcg acaatgtctt tcggtttata
    23821 taccgatagc tgatgaatta ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac
    23881 aaagtggctg acgccacgcg tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa
    23941 ttccacaaac aggtagttag ggaataacgg ttcactgact gcggtacgtt taccccgtac
    24001 gattttttcc agggtgatca tcggagcgag gcaattaacc gcctgtctct caaggtgttc
    24061 ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa taccaggatt gcataatgac
    24121 tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt tacgctaagt taattatatt
    24181 ccccggtttg cgttataccg tcagagttca cgctaattta acaaatttac agcattgcaa
    24241 agatgaacgc cgtataatgg gcgcagatta agaggctaca atggacgcca tgaaatataa
    24301 cgatttacgc gacttcctga cgctgcttga acagcagggt gagctaaaac gtatcacgct
    24361 cccggtggat ccgcatctgg aaatcactga aattgctgac cgcactctgc gtgctggtgg
    24421 gcctgcgctg ttgttcgaaa accctaaagg ctactcaatg ccggtgctgt gcaacctgtt
    24481 cggtacgcca aagcgcgtgg cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga
    24541 agttggtaaa ttattggcgt ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct
    24601 gtttgataaa ctgccgcagt ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg
    24661 tgcgccctgc caacaaaaaa tcgtctctgg cgatgacgtc gatctcaatc gcattcccat
    24721 tatgacctgc tggccggaag atgccgcgcc gctgattacc tgggggctga ccgtaacgcg
    24781 cggcccgcat aaagagcggc agaatctggg catttatcgc cagcagctga ttggtaaaaa
    24841 caaactgatt atgcgctggc tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg
    24901 tgcggcgcat ccgggcgaac gtttcccggt ttctgtggcg ctgggtgccg atcctgccac
    24961 gattctcggt gcagtcaccc ccgttccgga tacgctttca gagtatgcgt ttgccggatt
    25021 gctacgcggc accaaaaccg aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc
    25081 cagtgccgaa atcgtcctgg aagggtatat cgatcctggt gagatggcgc cggaagggcc
    25141 gtatggtgac cacacaggtt actataatga agtcgataat ttcccggtgt ttaccgtgac
    25201 gcatattacc cagcgtgaag atgcgattta ccattccacc tataccgggc gtccgccaga
    25261 tgagcccgcg gtactgggcg tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca
    25321 gttcccggaa attgtcgatt tttacctgcc gccggaaggc tgttcttatc gtctggcggt
    25381 agtgacgatc aaaaaacagt acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc
    25441 gttcttacgc cagtttatgt acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc
    25501 acgcgactgg aacgatgtga tttgggcgat taccacccgt atggacccgg cgcgggatac
    25561 tgttctggta gaaaatacgc ctattgatta tctggatttt gcctcgcctg tctctgggct
    25621 gggttcaaaa atggggctgg atgccacgaa taaatggccg ggggaaaccc agcgtgaatg
    25681 gggacgtccc atcaaaaaag atccagatgt tgtcgcgcat attgacgcca tctgggatga
    25741 actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc
    25801 gatccgacag agaaagcgca tgacaacctt aagctgtaaa gtgacctcgg tagcagctat
    25861 cacggatacc gtatatcgtg tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg
    25921 tcagtatttg atggtagtga tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac
    25981 gccggatgaa aaagggttta tcgagctgca tattggcgct tctgaaatca acctttacgc
    26041 gaaagcagtc atggaccgca tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg
    26101 agaagcgtgg ctgcgcgatg atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg
    26161 gttctcttat gcccgctcga ttttgctgac agcgttggcg cgtaacccaa accgtgatat
    26221 caccatttac tggggcgggc gtgaagagca gcatctgtat gatctctgcg agcttgaggc
    26281 gctttcgtta aagcaccttg gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg
    26341 ctggcgtggg cgtactggta ccgtgttaac ggcggtattg caggatcatg gtacgctggc
    26401 agagcatgat atctatattg ccggacgttt tgagatggcg aaaattgccc gtgacctgtt
    26461 ttgcagtgag cgtaatgcgc gggaagatcg cctgtttggc gatgcgtttg catttatctg
    26521 agatataaaa aaacccgccc ctgacaggcg ggaagaacgg caactaaaac tttattcagt
    26581 ggcatttaga tctatgtcgc atccggcaat tggtgcacga tgcctgatgc gacgctggcg
    26641 cgtcttatca ggcctacatt ggtgccggat cggtagaccg gataaggcgt tcacgccgca
    26701 tccggcaagt ggttaaaccc gctcaaacac cgtcgcaata ccctgaccca gaccgataca
    26761 catcgtcgcc agaccaaact gaacgtcttt gcgttccatc agattcagca gcgtggtgct
    26821 gatacgcgca ccggaacaac ccagcggatg acccagcgcg atcgcaccac cattgaggtt
    26881 gatcttctcg tcaatctgct ccattagccc gagatcttta atgcacggca ggatctgcgc
    26941 ggcaaaggct tcgttcatct caaacacgcc gatatcgctg gcagaaagcc ccgctttttt
    27001 cagcgccagc ttcgaggccg gaaccgggcc gtaacccata atcgatgggt cacaaccaac
    27061 gaccgccatc gaacgcacac gagcgcgcgg cttaagacct aattcatggg cgcggctttc
    27121 actcatcacc agcatggcag ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac
    27181 cgtaccgttt actggatcaa acgccggacg cagcgtggcg agggcttcca cggtggtttc
    27241 cgggcgaatc acttcgtcgt aattaaactg cttcaggacg ccgtcggcat cgtgaccacc
    27301 agttgggatg atttcatttt taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg
    27361 tgaccgcgcg gcaaaggcat cctgcatttc acggctgata ccgtgcatac gcgccagcat
    27421 ttctgccgtt aagcccatca tgcccgccgc tttggcgaca ttgcggctca ggccggggtg
    27481 aaaatcgacg ccgtgactca tcggcacatg gcctatatgc tccacgccgc caaccagaca
    27541 tgcctgcgta tcgccagtca tgatcattcg tgctgcgtca tgcagtgcct gcatggatga
    27601 accacacaag cgattgacgg taaccgccgg gacagagtgc ggcacttctg ccagcagcgc
    27661 cgcgttacgg gcgatgttaa aaccctgctc cagcgtctgt tgcacacaac cccagtaaat
    27721 atcgtcgagg gccgccgctt ccagcgccgg gttacgcgcc agcaggctac gcattaaatg
    27781 agcggagaga tcttccgcac gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg
    27841 ggtgcgaatt gcatcgacaa tgacaacctg ttccattgtg actccttaag ccgttttcag
    27901 gtcgccaacc ggacgggctg gctcaaccgg aggatagtac ggttcgttat gacgcgcttt
    27961 attacgcaga ccttccggca cttcatacag cgggccgagg tgctgatatt gctgtgccat
    28021 atcgaggtat tttgcgctac cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg
    28081 agggaagccc aggccgtaga ccagcgccat atccgcttcc gccggagtgg cgataatgcc
    28141 ctcttccaga cagcgcacca cttcgttgac catcgggatc atcatgcggg cgataatctc
    28201 ttcttcgctg aaatcgcgct tcggttggct cacttctgcc agcaggtctt caacggcggc
    28261 gtcttcttct ttcttcggct tacctttgct gtcttcttta taacgccaga aaccgaggcc
    28321 gttcttctga ccaaagcggt tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt
    28381 ctgcatccgc tgcgggaagc ctgctgccat gacagcctga gcgtgatgcg cggtatcaat
    28441 gcccacaacg tccagcagat atgccgggcc catcggccag ccaaactgtt tttccatcac
    28501 tttgtcgatc ttgcggaaat ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata
    28561 cgggaacagc acgcggttaa caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt
    28621 gcccatcttg ctcgcccagg cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc
    28681 gcgaataatt tctaccaacg gcattcggtg gaccgggtta aagaagtgca tcccgcagaa
    28741 gttttccggg cgttccagcg cgttggccag ttcgctgata ggaatggttg aagtgttaga
    28801 cgccagcacg gtatcctggc gtactttttg ttcggtttct gccagtacgg cttttttcac
    28861 tttcgggttt tcaacaaccg cttctaccac aatatccacg cggtcaaatc cggcgtagtc
    28921 gagcgttggg tggatcgtgg agatcacgcc agccagtttc agaccatcga ttttgccgcg
    28981 ctcaagctgc ttgttcagca gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc
    29041 gttgatatct ttcatgacaa ccggcacgcc tttccacgcg gactggtaag cgataccgcc
    29101 acccatgatg cctgcgccca gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag
    29161 tttcttcgct ttgcctttta catattgatc gttaaggaaa atgccgacca gtgcgcgagc
    29221 ttcgttggta tgcgccagcg ggacaaaact tttgttttcc agatttaagg cttcttcacg
    29281 accaaaacgg gccgcagctt caatggtttt tactgcggtg atgggggccg gataatgttt
    29341 ccccgccgtt tgcgcgacca ttcctttagc gatggtgaag ctcatggtgg cttcaatctt
    29401 gctcagtttt agcggttcct gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt
    29461 aatggcctgg cgtaaaatcg ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc
    29521 atccaccaga ccgattttca gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat
    29581 ttccagtgca ctgtcagcgc ccagcatacg tggcatacgt acagaaccgc caaagcccgg
    29641 catgatgccc agtttggttt ccggcagacc gatgcgcaga tccggcgtcg ccagacgata
    29701 atcggtcgcc agcacgcatt cgcagccacc acccagcgca tagccgttga cggcagcaat
    29761 ggtcggcacc ggcagatctt ccaggcgatt aaacacgcta ttggcaaagt gcagccactg
    29821 acttaactgt tcttcaggaa cgaggaacag ggacaaaaat tcggtgatat cagcaccgac
    29881 gataaaggct gctttgttcg aacgcagcag cagccctttt agatctgatt gctgttccag
    29941 cacgccgatg gcctcgccga ggctggcgac ggtcgcagtg tcgagtttat taactgaacc
    30001 tggggcatca aataccagtt cggcaatgcc atcttccagc cagtcaaggt acagggtgtc
    30061 gcctttgtaa agcatgtcag tctcctgaat ccgcaaggta atctggtcgt accagatgca
    30121 tcggagtgtg tgttttgtgt taaaaatatg caaataaaag attaaagaaa tgccgatctg
    30181 atcacgctcg gcagaaatca cgctctggat gaacgatgtg ctaagatgcg gagacttaag
    30241 gtcaaaaaaa cagaagggta aaaaatggaa tcactggcct cgctctataa aaatcatata
    30301 gctaccttac aagaacggac tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt
    30361 attcactccg gcgagctgtt caacgttttt ctcgacgatc atccctatcc gtttaaagtg
    30421 aacccgcaat tcaaagcgtg ggtgccggta actcaggtgc caaactgctg gttgctggtg
    30481 gatggcgtga acaagccgaa actgtggttc tatctgccgg ttgattactg gcacaacgtc
    30541 gaaccgctgc cgacctcctt ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc
    30601 gatggcattg gtagcctgct gcctgctgcg cgcggcaata tcggttatat cggtccggtg
    30661 ccggaacgtg cgctgcaact gggtattgag gccagcaaca tcaacccgaa aggggtgatc
    30721 gactacctgc attattaccg ctccttcaaa accgagtacg aactggcctg tatgcgtgaa
    30781 gcgcagaaaa tggcggtcaa tggtcatcgt gcggcagaag aagcgttccg ttctggcatg
    30841 agcgagttcg atatcaatat tgcctatctg accgcgaccg gtcatcgtga taccgacgta
    30901 ccttacagca acattgtggc gcttaacgaa cacgctgcgg tgttgcatta caccaaactg
    30961 gaccaccagg caccggaaga gatgcgcagc ttcctgctgg atgccggggc cgaatataac
    31021 ggctatgcgg ctgacctgac ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag
    31081 ctggtgaaag acgtaaatga tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc
    31141 agctatgtgg attaccacat ccagttccat cagcgcatcg ccaaactgct gcgtaaacat
    31201 caaatcatca ccgatatgag tgaagaagcg atggtcgaaa acgatctcac cggaccgttt
    31261 atgccgcacg gtattggcca tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg
    31321 caggatgata gcggtacaca cctcgcggca ccggcaaaat atccgtacct gcgctacacc
    31381 cgtattctcc agccgggcat ggtgttaacc atcgaaccgg gtatctactt cattgaatcg
    31441 cttctggcac cgtggcgtga agggcagttc agcaagcact tcaactggca gaaaattgaa
    31501 gcactgaaac cgttcggcgg cattcgtatc gaagacaacg tggtgatcca cgaaaacaac
    31561 gtggaaaaca tgacccggga tctgaaactg gcgtgatgga aagctggtta attcctgcgg
    31621 caccggtcac ggtcgttgaa gagatcaaaa agagccgctt cataacgatg ttggcgcata
    31681 ccgatggcgt tgaggcggcg aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg
    31741 cccgccacca ttgcgtggcg tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt
    31801 tctctgacga cggggagccg gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg
    31861 gcagcggcgt cggggaaatt accgctgtgg tagtgcgcta ctacggcggc atattgcttg
    31921 gcaccggtgg gttagtgaaa gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa
    31981 cgacccaacg caagacgcca ttaaccgaat atactttgca atgtgaatat agtcagttaa
    32041 ccggcattga agcgttgctg gggcagtgtg acggcaaaat tatcaacagt gattatcagg
    32101 cattcgttct gctgcgggtg gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc
    32161 tggcggattt tagccgtggt tcattgcaat tgttagcgat tgaagaataa tccccacttc
    32221 gttttgcaga actaaggaag cggcagagat gcattttcgc gccattaccc gaatcgttgg
    32281 actactggtc atcttatttt cagggaccat gattatccct gggctggtag cactcatcta
    32341 ccgggatgga gcgggccgcg cttttaccca gacctttttt gtcgccctcg ccattggctc
    32401 tatgctgtgg tggccgaacc gcaaagagaa gggcgaactg aaatcccgtg aggggtttct
    32461 gatagtggtg ctgttttgga ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc
    32521 ggaaagcccg aacctcacga ttaccgatgc gttttttgaa tctttctctg gcctgaccac
    32581 caccggggcc actacgctgg tggggctgga ttcgctccct catgccatcc tcttttatcg
    32641 ccagatgctg caatggtttg gcgggatggg gatcatcgtg ttagcggttg cgatactgcc
    32701 tatcctcggc gtgggtggga tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga
    32761 taacaaaatg cgcccgcgaa ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt
    32821 gctgaccgtc gcctgtgcgc tggcgttgtg gtttgccgga atggatgcct ttgatgccat
    32881 cggccatagc tttgcgacta tcgctattgg cggcttctcg acacatgatg ccagtatcgg
    32941 ttatttcgac agtccgacta ttaacactat cattgctatc ttcctgctga tctccggctg
    33001 taactacggt ctgcactttt cactgttaag tgggcgtagt ctgaaggttt attggcgcga
    33061 tccggaattt cgcatgttta tcggcgtaca gtttacgctg gtggttattt gtacactcgt
    33121 actgtggttt cataatgtct acagttcggc gctgatgaca attaaccagg cgtttttcca
    33181 ggtggtatcg atggcgacaa ccgccgggtt tacgactgac agcattgccc gctggccgct
    33241 ctttttgccg gtactgcttt tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg
    33301 cggtggcctg aaagtgatcc gcatcctgct gctgtttaag caggggaacc gtgagctgaa
    33361 acgactggtg catccgaacg ccgtctatag cattaagctg gggaatcgcg cactgccgga
    33421 acgtatcctc gaagccgttt ggggattttt ctccgcctat gcattggtgt ttattgtcag
    33481 tatgctggcg attatcgcca cgggcgtgga tgatttttct gcctttgcct cagttgttgc
    33541 gacattgaat aacctggggc cagggcttgg cgtggttgct gataacttta ccagtatgaa
    33601 cccggtggct aaatggatcc tgattgccaa catgctgttt ggtcgtctcg aggtctttac
    33661 attactggtg ctctttaccc cgactttctg gcgtgaatga tggagtaata cgtgaaaaca
    33721 ttaattcttt tctcaacaag ggacggacaa acgcgcgaga ttgcctccta cctggcttcg
    33781 gaactgaaag aactggggat ccagacggat gtcgccaatg tgcaccgcat tgaagaacca
    33841 caatgggaaa actatgaccg tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac
    33901 tctgcgttcc aggaatttgt caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc
    33961 ttttattccg taaacctggt ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc
    34021 tacgcgcgga agtttctgat gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc
    34081 ggggcgctgc gttacccacg ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg
    34141 aagatgtcag gcggtgaaac ggatacgcgc aaagaagttg tctataccga ttgggagcag
    34201 gtggcgaatt tcgcccgaga aatcgcccat ttaaccgaca aaccgacgct gaaataagca
    34261 taaagaataa aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa
    34321 taactcccta taatgcgcca ccactgacac ggaacaacgg caaacacgca gccgggtcag
    34381 cggggttctc ctgagaattc cgacagagaa agcaaaaata aatgcttgac tttgtagcgg
    34441 gaaagcgtat tatgaacacc ccgcgc
//
LOCUS       DAJTRM010000035        34382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRM010000035 DAJTRM010000000
VERSION     DAJTRM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.035"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1996
                     /locus_tag="OO322_001799"
     CDS             <1..1996
                     /locus_tag="OO322_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5906710.1"
                     /translation="RMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQ
                     GTPVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLT
                     RTELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLA
                     NADDVSAFELGNNWTADLGRVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYR
                     TNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQ
                     QSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYL
                     SFSLSDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVG
                     GLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNL
                     GASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQS
                     GFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAV
                     KKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSG
                     DVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            1993..3081
                     /locus_tag="OO322_001800"
     CDS             1993..3081
                     /locus_tag="OO322_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5906711.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3169..4467)
                     /gene="exuT"
                     /locus_tag="OO322_001801"
     CDS             complement(3169..4467)
                     /gene="exuT"
                     /locus_tag="OO322_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5906712.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4952..6364
                     /gene="uxaC"
                     /locus_tag="OO322_001802"
     CDS             4952..6364
                     /gene="uxaC"
                     /locus_tag="OO322_001802"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5906713.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6379..7866
                     /gene="uxaA"
                     /locus_tag="OO322_001803"
     CDS             6379..7866
                     /gene="uxaA"
                     /locus_tag="OO322_001803"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5906714.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILSTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7949..8500
                     /locus_tag="OO322_001804"
     CDS             7949..8500
                     /locus_tag="OO322_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5906715.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8505..9749)
                     /gene="sstT"
                     /locus_tag="OO322_001805"
     CDS             complement(8505..9749)
                     /gene="sstT"
                     /locus_tag="OO322_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5906716.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10345..11310)
                     /gene="alx"
                     /locus_tag="OO322_001806"
     CDS             complement(10345..11310)
                     /gene="alx"
                     /locus_tag="OO322_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5906717.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11581..12579)
                     /locus_tag="OO322_001807"
     CDS             complement(11581..12579)
                     /locus_tag="OO322_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5906718.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12658..13350)
                     /locus_tag="OO322_001808"
     CDS             complement(12658..13350)
                     /locus_tag="OO322_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5906719.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13427..13930)
                     /locus_tag="OO322_001809"
     CDS             complement(13427..13930)
                     /locus_tag="OO322_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5906720.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14015..15151
                     /gene="rlmG"
                     /locus_tag="OO322_001810"
     CDS             14015..15151
                     /gene="rlmG"
                     /locus_tag="OO322_001810"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5906721.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15436..15750
                     /gene="higB"
                     /locus_tag="OO322_001811"
     CDS             15436..15750
                     /gene="higB"
                     /locus_tag="OO322_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5906722.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15747..16163
                     /gene="higA"
                     /locus_tag="OO322_001812"
     CDS             15747..16163
                     /gene="higA"
                     /locus_tag="OO322_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5906723.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16208..18226)
                     /gene="fadH"
                     /locus_tag="OO322_001813"
     CDS             complement(16208..18226)
                     /gene="fadH"
                     /locus_tag="OO322_001813"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5906724.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18452..20803)
                     /gene="ygjK"
                     /locus_tag="OO322_001814"
     CDS             complement(18452..20803)
                     /gene="ygjK"
                     /locus_tag="OO322_001814"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5906725.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20820..21239)
                     /locus_tag="OO322_001815"
     CDS             complement(20820..21239)
                     /locus_tag="OO322_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906726.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21217..21570)
                     /locus_tag="OO322_001816"
     CDS             complement(21217..21570)
                     /locus_tag="OO322_001816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906727.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21571..21891)
                     /locus_tag="OO322_001817"
     CDS             complement(21571..21891)
                     /locus_tag="OO322_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906728.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22025..23458)
                     /locus_tag="OO322_001818"
     CDS             complement(22025..23458)
                     /locus_tag="OO322_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5906729.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23521..23970)
                     /locus_tag="OO322_001819"
     CDS             complement(23521..23970)
                     /locus_tag="OO322_001819"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5906730.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23967..27053)
                     /gene="ebgA"
                     /locus_tag="OO322_001820"
     CDS             complement(23967..27053)
                     /gene="ebgA"
                     /locus_tag="OO322_001820"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5906731.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27236..28219)
                     /gene="ebgR"
                     /locus_tag="OO322_001821"
     CDS             complement(27236..28219)
                     /gene="ebgR"
                     /locus_tag="OO322_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5906732.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28437..28768
                     /locus_tag="OO322_001822"
                     /pseudo
     CDS             28437..28768
                     /locus_tag="OO322_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28810..30189)
                     /gene="ygjG"
                     /locus_tag="OO322_001823"
     CDS             complement(28810..30189)
                     /gene="ygjG"
                     /locus_tag="OO322_001823"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5906733.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30607..32127
                     /gene="aer"
                     /locus_tag="OO322_001824"
                     /pseudo
     CDS             30607..32127
                     /gene="aer"
                     /locus_tag="OO322_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32281..32904)
                     /gene="nfeR"
                     /locus_tag="OO322_001825"
                     /gene_synonym="yqjI"
     CDS             complement(32281..32904)
                     /gene="nfeR"
                     /locus_tag="OO322_001825"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5906734.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33181..33926
                     /gene="nfeF"
                     /locus_tag="OO322_001826"
                     /pseudo
     CDS             33181..33926
                     /gene="nfeF"
                     /locus_tag="OO322_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33980..34055)
                     /locus_tag="OO322_001827"
     tRNA            complement(33980..34055)
                     /locus_tag="OO322_001827"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34020..34022),aa:Ile,seq:cat)
     gene            34180..>34382
                     /locus_tag="OO322_001828"
     CDS             34180..>34382
                     /locus_tag="OO322_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906735.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGIT"
BASE COUNT         8378 a         8863 c         9021 g         8120 t
ORIGIN      
        1 tcgcatggac aaccgcacac tatttaatgc acaaggcggg aactttacct ttaactttct
       61 gccactcggt gcaatcgatg ggatgcgtat cgggtcgaca ctgagctatt taaaccaggc
      121 gcaaagccag caaggaaccc cagtaatggt tctgctttcg cgcaattctc gtgttgacgc
      181 ttatcgtaat gagcaacttt tgggatcgtt ttatctcaat agtggttcgc aatttattga
      241 taccagttcc tttccgccag gcagctacag cgtggcatta aaagtctacg aaaataacca
      301 actcacccgc accgagcttg tgccgtttac caaaaccggc ggtctgactg acggaaatgc
      361 gcaatggttc ttacaggcag gtaaaactac atcacaggtt tctgatgatg aaagttcagc
      421 ttatcagcta ggagtacgcc tgccattaca tccgcaatat gagctctacg cagggctggc
      481 gaatgctgat gatgtgagtg ctttcgagtt aggtaataac tggacggcag atttaggcag
      541 ggtggggaat cttgcaatca gcgccagcgt gttccgtaac gatgacggcg gcaaaggtga
      601 tatgcaacag gccaactgga gtaatccggg atggccgacg ttgggctttt atcggaccaa
      661 ctctgacggc gatgcttgta caaccgacag cagagagagc tataacgcct taagctgtta
      721 tgaaagtatt tccgcgacag tttcactgaa ttttgtcggc tggaatatga tgctgggtta
      781 tacctgcaca caaaataaca ctgatgatag tttgcgttgg gataaacagc agagctttga
      841 aaataactat cttcgccaga caacagcgca aagtatctct gaaactgtac aacttagcgc
      901 ttcccgcgct attgtgatgc gtgactggat tttgagtacg tcagtcggtg ttttccatcg
      961 taatgacaac ggtggcgata acgacgacaa cggcttgtac ttatcgtttt cgttatctga
     1021 cacgccaacg atggatagca ataacaacag ccattcaacc aatgtgccta cggattatcg
     1081 ttatagcgaa caggatggcg atcaaacgtc atggcagtta tcgcatacct tttataacga
     1141 ttcattcagc cataaagaac ttggcgtaac cgtcggaggc ctgaacaccg ataccataaa
     1201 cagcgcggtt aacgggcgtt gggatgggca atacggaaat gtctacgcta ccgtatctga
     1261 tagttatgac cgtaagaatc atgatcatct ttcggccttt acgggcactt atagctctac
     1321 gctggctgtc agtcgctatg gcgttaattt gggagccagt ggtacagacg atttgctggg
     1381 tgcggtactg gtggatgtga aaggcttctc tgaacaggat gaagagagtc aggatctgca
     1441 actcgaagcg cgggtggcag gcagccgaac tttgcagctt ggtcaaagtg acagtgtgtt
     1501 gttcccttat cctggatttc agtctggttt tgttgaggtt aacgacagta gccagggcaa
     1561 tcagcagggg acaacaaaca tcattaacgg tgcagggaat cgtgaattaa tgttgttgcc
     1621 tggcaagctg cgctatcgcg aagtgtctgc cagctttaac tacaactata tcggtcgttt
     1681 attactgccg gcagcggtga aaaaattccc gattgtgggg ctgaacagcg ccatgttact
     1741 ggtagctgaa gatggcggat ttacacttga aattaacggc agcgaaaaag agctctatct
     1801 gctttccggg cagcaattcc ttaagtgtcc actgagtgtt gtaaagaaac gcgccagcat
     1861 tcgttacagc ggagatgtta cttgtagtgt ggtgacttat tcacaattac cggagtcgat
     1921 tcaggttcag gcacagttga aacagcctaa attacgtgga aacgttcaga cggcgcaaag
     1981 ggaggttgca ccatgagaaa tcgattgatt gcggcgatat tgggtttgtt tggcacgctc
     2041 actggcgttc aggcagctcc tgacgtgacc agtgaaatta cgtatgattt ggcatctggt
     2101 agagcggatt attacttctg gaaggatgaa gcgtcggcag gaaataatgg atatatgtgg
     2161 tatgaatgtt cgtatcctga cctccaacaa acctgtacag ctaatggaaa tatatcgaca
     2221 gtacaaatct atttaactga acaacgcagt gggatgcgtt ggccggtaaa actcaaagga
     2281 tttaaaacag ccattgtaag tagcgatgaa gcgccgctag gatgcaaggg gggcaaaggg
     2341 cttcagacga atcttaagga ttctaataga tcttcatgta cagaagatgg tcaacattat
     2401 tatatatacg atacaaagtt tcttacgctc taccttgagc agacagagat gaagaatttg
     2461 ccgattggtg gcgtctggaa ggggaaagtt aaattacatt cgaacagccc ggcccaggac
     2521 tatttcgcaa atattaccct gaatacgctc gaccccaacc atattgacgt gttcttcccg
     2581 gagttcgccc acgccacgcc aagggtgcag ttagacttgc atccaacagg aagcgttaac
     2641 ggcagcaact acgcgcaaga tctgaccatg ttggacatgt gcctgtacga tggttttaac
     2701 ggtaatgcca tcagttatga aatcatgctc aaagatgaag ggcgaccagc tgcagggcgc
     2761 agagacggtt acttctctat ctatcgtcag ggagggacca ccaccgacga gggagaacgc
     2821 attgattacc gggtcaaaat gtacaacccg gaaaccggtg ggcaaattga tgtgcgcaat
     2881 aatgaaaata tggtctggaa cagcattaac ctgaaacgtg tgcgtccggt ggtactgccc
     2941 ggtattcgct atgccgtgat gtgtgtgccg acacccttga ctctggcagt cgataaattc
     3001 agcgtgatgg ataaacaggc cgggtactac atgggcaaat tgtcagtaat ctttacgcct
     3061 tccttgccaa ccatcaatta accctgcaaa ccggacaaac gcttttttct atataaacga
     3121 cgaagccgcc tgataaggcg gcttcgtcgt tataacttac gttaaacgtt aatgttgcgg
     3181 tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc ggtttgttct gcaatacggt
     3241 ccagataacc agcgcaccca gcaggtcaaa tactgccaga actgcgaaca gcgggctgaa
     3301 gccgatggtg tcagccagtg caccgacaac cagcgcaaac agcgtacttg ccagccatgc
     3361 ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta cgaccgaaca cgtcagaaga
     3421 gagcgtaatc agcgcgccag acagtgcctg gtgggcaaaa ccaccgatac acagcagcat
     3481 aattgcgaca tacgggttgg tgaacagacc gatcataccc gggccaatca tcagcacagc
     3541 acccagcgtt acgaccatct tacgggaaac gatcaggttc acaccaaacc aacgctggaa
     3601 cagcggcggc aggtaaccac cgaggataca accgaggtca gcaaacagca tcggcatcca
     3661 ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact ttaaacatga acagcgggat
     3721 ccacgcgtta aaagtacccc aggccggttc tgcaaggaaa cgcggcagtg cgatacccca
     3781 gaactgacgg ttacgcagga tctgaccaac ggacattttc ttcgccgtgc tcacctggtg
     3841 ctgggcttcc tgaccattaa taatatagtc gcgttcttca tcggtcagat gcttctggtc
     3901 gcgcggatgt ttatagaaaa tcagccatgc catcgcccag ataaagctca atgcaccgga
     3961 gatgataaat gccatctgcc agctgtgcat cacgattgcc cataccacca gcggcggcgc
     4021 aatcatcgca ccaatcgaag aacctacgtt aaagtagcct actgcgatgg aacgctcttt
     4081 cgccgggaac cattcggagc tggctttcag acccgccggg atcatcgctg cttccgcggc
     4141 accgaccgca ccacgagcaa cagctaggcc accccagcta cctgccagcg cggttgcacc
     4201 acagaacacg gcccacagca cagcaaacat tgcataaccg attttcgtac ccagcacatc
     4261 cagtacataa cctgctaccg gttgcatgac cgtataagca gcagaatagg ctgcgatgat
     4321 ataggaatac tgttgggtgg agatgtttaa ctcttccatc agagttggcg cagctgccgc
     4381 cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg cgatcatata
     4441 ccaacgtaac cctttaattt tacgcatgta aaacctcatc gttttgttat atccgcagga
     4501 agcgcggcca tcatcccgcc agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa
     4561 aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg cttccgtacc
     4621 ggttaacccg attcatctaa caaatgaatg aatcggcaat ttgcattccg tcgacttatg
     4681 atttgcgacg gcagaaagat aacttgtcat acaaccttaa aaggtgagag ccatcacaaa
     4741 tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg gcgcggcctg
     4801 cagcgagatt taccactttg agagtaattt ttttaactac gtttattgat ctaactcacg
     4861 aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc accataagca
     4921 agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg aagatttcct
     4981 gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc agccgatttt
     5041 cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt ttaaaaacct
     5101 gtatgacatc tggttgaagg gcgatcacta caaatggcgc gctatgcgta ccaacggtgt
     5161 ggctgagcgt ctgtgtactg gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc
     5221 gactgttccg cacactatcg gcaacccgtt ataccactgg acgcacctcg aactgcgtcg
     5281 tccgttcggt atcactggca aattgctttc tccgtcaact gccgatgaaa tctggaacga
     5341 atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc agcagatgaa
     5401 cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc acgcagagat
     5461 cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt
     5521 caatatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag tttccgatac
     5581 cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg atcacttcgc
     5641 cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa
     5701 cgaagcggaa ctggacagca ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca
     5761 cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg
     5821 cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc gtcagttcaa
     5881 actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg cggaagagtt
     5941 gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca ttctctactg
     6001 cctgaacccg cgcgataacg aagtgctggg caccatgatc ggtaacttcc agggcgaagg
     6061 tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga aagacggtat
     6121 ggaacgtcag atgacccaac tggcgcagct cggtctgctg agccgctttg tcggtatgct
     6181 gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg
     6241 ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg gcggacatca acctgctggg
     6301 cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa
     6361 ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa cgtcgcggtc
     6421 gctttagcag atttggctga aggcacagaa gtcagtgtcg ataaccagac tgttacgctg
     6481 cgccaggatg ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa aggggccaac
     6541 gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc gggggaacac
     6601 gtccacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg ctatcaactt
     6661 gattttcagg atctgcctgc gcaagcggca gatcctgaag tgcagatcta tcgtcgcgct
     6721 aacggcgatg tcggggtgcg taacgagctg tggatcctgt caaccgtggg ctgtgtcaac
     6781 ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga ccaacaacgc cgaaggtacc
     6841 gacggcgtgt tcctcttcag ccacacctat ggctgctcac aactgggcga cgatcacatc
     6901 aacacccgca ccatgctgca aaacatggtg cgccacccga acgcgggcgc agtgctggtg
     6961 attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgtt gggcgatatc
     7021 gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat cgaagccggg
     7081 atcgagcatt tgcatcagct gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa
     7141 ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc tggtattact
     7201 gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg tactaccgta
     7261 ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca ttgccgcgac
     7321 gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact tcaaacagta ctttattgcc
     7381 catgatcagc cgatctacga aaacccatca ccggggaaca aagcgggcgg tatcaccacg
     7441 ctggaagaca aatcacttgg ctgtacccag aaagcgggtt ccagtgtcgt ggttgacgtg
     7501 ctgcgttacg gcgagcgtct gaaaacgcca gggctgaact tgttaagtgc gccgggtaac
     7561 gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct gttcagtact
     7621 ggtcgcggca cgccatacgg tggatttgtg ccgacggtga aaatcgccac caacagtgaa
     7681 ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa
     7741 gcgatgccgc agttgctgga agaatttatc gacaccatcg ttgagtttgc caacggtaag
     7801 caaacctgca acgagcgtaa cgacttccgc gaactggcga tcttcaaaag cggcgtaacg
     7861 ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga agcgccgttt
     7921 gcttttccat acctgctaag gatttatcat gaccgcgtat tggctggccc agggcgtggg
     7981 tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa
     8041 aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc tgggcaccta
     8101 tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct tacgcacgcg
     8161 cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc tcgcgaagtt
     8221 ccatcatcct gtcgaactat tgccggttat cggtacgatt gtcagtacct gggcgctgtt
     8281 ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat
     8341 tcacaacttc tgggcggggt cgataggcgg cacgatgatt gagggtagtt ttctgcttat
     8401 gaatggcctg aatatcattc gtttctggcg gatgcagaaa aggggaattg atccgtttaa
     8461 agtagagaaa acccctcccg ccatagacga aaggggttaa acaattaatt acgcagggcg
     8521 ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag cacgtcagtt
     8581 gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgataataaa gccgacggca
     8641 accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag tgggatcagc
     8701 agcaaagaac cccccgccac gccggatgcg ccacaggcac acagagaagc cactacgctc
     8761 aacagcagcg ctgtgggcag atcgaccgga atacccagcg tattaaccgc agccagcgtc
     8821 aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggctcccag cggaatagaa
     8881 actgaatagg tatcgcgatc cagattcagc ttttcacaca gcgccatatt caccggaatg
     8941 ttcgctgcag agctgcgggt gaagaaggca tacacaccgc tttcgcgcag gcacagcagc
     9001 accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt aaccaccagc
     9061 gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg
     9121 gagaaaccgg tggttgccag ggtagaagaa accagcccaa aaataccgaa cggtgcgaag
     9181 cgaatgacca gtttcaccat aaaggtaacg gcattcgaca tatcattgac caggtttttg
     9241 gtggtctcgt taccgtgacg cagtgcgaag ccgaggccga tcgcccacac cagaatcccg
     9301 atgtagttac ctttcagcag ggcgtcgatg gggttggaaa ccatgctcat taccagtccg
     9361 cgcatcactt cgacaatgcc tgacggcggc gaaatatcac ccgcgctact ggacaagtgc
     9421 agggtagaag ggaaggcaaa gctgaagact actgcggcca gagctgcaga gaaggtgccc
     9481 agcagataga ggaacaaaat aggacggata ttggttttct gcccgtgctg gtggttagca
     9541 atagatgcca ttaccagcat caacaccagg atgggggcaa cagctttcag tgcgccgacg
     9601 aacaaagtac ctaacagacc aacagcttcc gccgcgggtt ttgagatcca tgccagaaga
     9661 atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc cagacgccgg
     9721 aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat
     9781 ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt
     9841 ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag aggtcatgtt
     9901 ttgcaactta atggttgcat ttatttaaca taaacagagg gaaggactgg ctactaaggc
     9961 tgattagata acatatgggc aaaactattt ggaatatgag aacggttgcg ctcctttaac
    10021 gtgttatgcc atataaagtc actggcgcag ggagcgcgca gggggcggcc aatcgccgcc
    10081 gccccctgca cccccaggct ctggcgaaca aaatcgccgc tgcgcggttc cctcagccta
    10141 tcccttgcgg ctaccgggtc gggcgcaagg taacgtccct gtaagatgcg ccctcgcccc
    10201 ggcctccagg gaacgcttcg gcgattttga cgccaccaac acccgtgcgg tctttttgta
    10261 aagagctata agccgattac ataattaata gctcaccttt gtctggaatg agttatctct
    10321 aggaatggct taatatacag cgaatcattc tccccgctgc ttatcatgcc gataattcac
    10381 ccaggcgttg ataataaacg tcatcaccag aatgccaaac accacgccca gcgagacggc
    10441 gattggaata tggtagaagt cgacaatcag catcttgata ccgataaaca ccagaatcac
    10501 cgccaggcca tatttgagca tcgagaaacg ctctgctacg cccgccagca ggaaatacat
    10561 cgcacgcagg ccgaggattg caaacaggtt tgaggtcagc acaatgaacg ggtcagtggt
    10621 cacggcgaag atagccggaa tgctatccac ggcaaaaatc acgtcgctca attccaccag
    10681 aatcagtacc agcatcagcg gtgtggcgta cagcaatcca ttcttacgca caaagaaatg
    10741 ctcgttgtcg atggtgtcgg tcatgcgcaa atgaccgcgt agccagcgca ccagcggctt
    10801 gtcgccaatg cctgattcat cttcatgggc gagcgccatc ttcacgccgg taaacagcag
    10861 gaaggcaccg aagatataca ggatccagtc gaactgcgaa atcagccagc tgcctgtgaa
    10921 gatcatgatg gtacgcagaa caatcgcccc gagcacgcca tacaccagca cgcgacgttg
    10981 taatgccgcc ggaacggaga aatagctgaa caacatcagc cagacaaaga cgttatcgac
    11041 cgccagcgat ttctcaatca gataacccgt gagaaaggcc agggcctgtg gatcggcaac
    11101 ggcgcgacct tcggtttgca ccagatacca ccagaaagcg gcgttaaaca gtaacgacag
    11161 cgtcacccag accagcgacc aggccgcagc ctgtttcatg gtcatggcat gtgccccacg
    11221 acgcccctgc aacaacaggt cgatagccag cataatggcg acaacaacag cgaatccgcc
    11281 ccatagcaac ggcgtgccga cagtattcat agaagttcct tacacataaa aaaacggcca
    11341 acgtcggaag acgtcagccg ctgcttttta tgcatagacc tcgccttccg gcaaggtctc
    11401 acttacaaca aaaaaggaat acgtctgcgt attcctttcg ggttgcccgg tgaccggatg
    11461 tggtttttca ctcatcgtaa tgacgatcga ccggcaatga agttactccc ctttgcaggt
    11521 aacaaagtat gacagaccat tcagtcagtc aatggtctgt tacatacctt ttacactgtt
    11581 ttactttggc aagtatagtt ttacgctatc tgccggaaaa atcaccccag tttggcggcg
    11641 gatctcggtc agcagtttgg ctgtgatgcg actgaccgcc agccctggat gatccaccag
    11701 atgctcatcc accagggtag cgaacagctc tgcttcgtag agcatggtat taatatgctg
    11761 cggctgggtg agatcctgca tctggctgcc acgcggcaca aagcagactt tctggcattc
    11821 agacagtttt tcaatcacca gcgatcccgc ttcgccctga atctcgcttc ccaggacaga
    11881 atcactgacc tttgagtgct gcaaggtgac gctgaaatca ccgtaatcca tcaccaccac
    11941 gccgtgggcg tcaacgccgc ttgccagcaa actcgccgtt gcctgcacgc ttttcggctc
    12001 accaaataac gccaccgccg acgccaggca gtaaaagccg atatccataa ttgaaccgtt
    12061 agagaatgcc ggattaaagg tgttgggatt ctcaccgtcc aggtaacgtt gataccgcga
    12121 ggaatactgg caatagttga aaaagacttt acgcagtttg cctgctttcg gcagcgcctg
    12181 gcgcaacaaa tgaaagttcg gcaggcaggc ggttttaaat gcctcaaaca gcaccacctg
    12241 attttcgcgc gcacaggcaa tggcggcatc cacttccgcc agattcgacg ccagcggttt
    12301 ttcgcaaatc acatgaattt tatggctaag gaaaagttgt gtctgggaaa agtgcaggga
    12361 attcgggctg gcaatataca ccgcgtcaat ggcatcgctt tccgccatcg cttccagcga
    12421 cgtaaacaga tgctcgacag aaaaatcatt ggcgaagtgc tgagcctgct caaggctgcg
    12481 ggaatatacg gcggttaact tgtatttacc gctctcatgg gcggcctcga cgaactggcg
    12541 agtgatccag ttcgtaccaa tcacagcgaa acgtatcata acgcgtggat actccataaa
    12601 gggattctgt cgtcaatgta gcacgccttt ttcatcttcg acgtgcgcag atacgcatta
    12661 ttctttcgcc ggacagccat gctcactaaa cggcccaatc atgaccgatg gcccgagata
    12721 tttcggctgg cgatggagga tttttgcgtc aatcaccgcg ctgacacgag cgagtttctc
    12781 ccgcagttga gtataaaaac cgtgcttcat attgaggtct ggcgcgttaa acccgatagc
    12841 gtcgatgccg tactgttttg ccagccagat ggtgcgttgg ttatgaaatt cttgcgagat
    12901 aatggtgata tggttttccc caaacacctt tttggcacgc actaccgaat ccagcgttga
    12961 gaatccggca tagtcgcaaa agatcacttt cgccggtacg cctttagcga tcaaagcctg
    13021 ctgcatacct gatgcttcat cgtaattttt acgtccgtta tcgccactca ccagtagcca
    13081 tttcactttt ccggcgtggt acaacgctgc tgcggtatca atacgtcggg taaagtagcg
    13141 attacccggc ctcgccccca gcaataaacc cacgttacgc gccgggacgg cgttgacatc
    13201 gccccaggtc agctgcttgc tggcgttgac cataacgcga tcggcgataa aaatggtcgc
    13261 tcctgccaca agtaacagta agcaggcaat ctttagagtc cggcgagaaa aacaaatacg
    13321 gagaagaagg cgggcaaatg cgcgcagcat ggtcgttatc cttaacgaaa ggggggaggt
    13381 cgattttagg cgctaaagaa agaaaagata acgcctgaca tcattttcag attttgtcct
    13441 gcccaagcga caactgcgtt agccacagcc gggtgtcgaa ctcaagctgg tggtactgcg
    13501 gctccatatg gcagcacaat tgatagaacg ctttgttatg ctctttctcc ttcaggtgcg
    13561 ccagctcgtg caccacgatc atgcgcaaaa acggttccgg cgcgttgcga aacacggtag
    13621 cgacgcggat ctctgctttt gctttcagct tgctgccctg cacacgagaa acagcggtat
    13681 gtaacccgag cgcgtttttt agcacatgga tcttattgtc atacatcact ttattgatcg
    13741 gcggggcatt acgcagaaac tgatttttca gatcctgagt atattgccag agagctttat
    13801 cggtggcgta gtcgtgtgtt cccggatagc gttttgccag cacatcgccc agacgctgtt
    13861 cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg ataaccctga agataagtaa
    13921 gattgctcat aaccgcccac gaaaaagaga aaaagggtat actcacgcac ccttttcagg
    13981 ggcaacgtcg aaattttatc attcaggagg gccgatgagc cacttagaca acggtttccg
    14041 ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt aacccgctac aggcgtggga
    14101 agcggcagat gaatatttgc tgcaacagtt ggacgacaca gaaatcctcg gcccggtgtt
    14161 gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg gcggaacata agccgtacag
    14221 cattggcgac tcatacatca gtgaactggc gacgcgcgag aatttacgcc taaacgggat
    14281 tgatgagtcg agcgtgaagt ttctcgacag caccgccgac tacccgcaac agccgggtgt
    14341 ggtgctgatc aaagtgccga aaacactggc attgctggaa cagcaactgc gtgcgctgcg
    14401 caaagtggtc acgtcggata cacgtattat tgccggtgcc aaagcccgtg acattcacac
    14461 ttccacgctg gaactgttcg aaaaagtgct cggcccgacc accaccacgc tggcatggaa
    14521 aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg ctggccgatg cgccgcagac
    14581 cgttagctgg aagctggaag gtactgactg gactatccat aaccatgcga atgtcttctc
    14641 ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa catctgccag agaatctcga
    14701 aggggagatt gtcgatctcg gttgcggtaa tggcgttatt ggtctgacgc tgcttgataa
    14761 aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg atggcggttg cttccagccg
    14821 tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc agcgagttta tgatcaacaa
    14881 cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg ctctgcaacc ctccgtttca
    14941 ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg ttccactacg cccgccgctg
    15001 cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc catctggatt acttccataa
    15061 actgaagaaa attttcggca actgcaccac tattgcgacg aataataaat ttgtggtgct
    15121 gaaagcggtg aagctgggac gtcgtcggta agattgggag ttacctgccg gatgcggcgt
    15181 aaacgcctta tccggcctac gttcagcacg ttacccccag gccggataag acgcggcaag
    15241 cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc ttccataaaa caaaaaatcc
    15301 ctgtcaccat cctctacatt ctcttgttta gcgtttttct acgtttattc ttccgtcaca
    15361 cagataaatt catccgttgc caatctgtca acggatgtta tcatattgcc aatttgtcaa
    15421 cggacgtgat gaagaatgca cctgataact caaaaagcat tgaaagatgc tgcggaaaaa
    15481 tacccgcaac ataaaacgga gttggtggct ctggggaaca cgattgctaa gggatatttc
    15541 aaaaaacctg agtcattaaa agcagtattc ccatctctgg ataacttcaa atatctggat
    15601 aagcattatg ttttcaatgt tgggggcaat gaattacgtg ttgtagcaat ggtctttttt
    15661 gaatcgcaaa agtgctacat acgtgaagtt atgacgcata aagaatacga tttctttacc
    15721 gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc cgacatcttg caagcaggag
    15781 aaaagctaac tgctgtggca ccttttctgg cgggtattca gaacgaggaa caatacaccc
    15841 aggcgctgga actggtagat catctgctgc tcaacgatcc tgaaaacccc ttgctggatc
    15901 tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc cgaatttgcg gaatttaatg
    15961 ccatggctca agccatgcct ggcggtatag ccgtgattcg tacccttatg gatcaatatg
    16021 gtttaaccct ttccgatctg ccggaaattg gcagtaaatc tatggtgtca cgcgttttga
    16081 gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt ggcaacgcga ttcggcattt
    16141 ctcccgcctt gtttattgat taacgcgacg tgccgaatgc gttgcatccg gcgcttcaaa
    16201 taacgattta aatctccagc gccagccgtg ttccctgggc aattgcccgt cgtgcgtcca
    16261 gctccatagc cacatcgcag ccgccaatta aatgcacagt tttcccgcta tcaatcagcg
    16321 gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat caccacattg tccactgcta
    16381 atacctgcgt ttcgccgttg atcaccacat gcagcccgtc atcgtcaatc ttctgataac
    16441 ttacgcctgg gatcattttc acaccccgcg agagcagggt ggtgcgatgg atccagccgg
    16501 tggttttgcc taacccctgt cccggtttgc tggctttgcg ctggagcatc acaatttgcc
    16561 gtgggctacg cgggatctgc attccctgcg ggcttaagcc accagcctgt tgtaggctgc
    16621 tgtcgatccc ccactcatta cagaacctgg cgatattctg gctggtggat tcgcccggct
    16681 gacttaaata catcgccgta tcaaaaccaa tcccgccaca accgatgatg gcaaccttgt
    16741 tgcctaccgg cgctttgtcg cgcagtacat cgagataact caataccttc ggatgatcga
    16801 tcccgtcgat aggcggaaca cgcggcacga tcccactggc gaggatcgtt tcatcgaacg
    16861 cctgtaactg atccgccgtc acggtgtgat tgagtttcag cgtcacgccc gtcacttcga
    16921 tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc tttgccgggg atctgtttgg
    16981 cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa caatgttacc tgatgcccac
    17041 gcgccgccgc gttaatggca aacgccagcc cagcaggtcc agcgccgacc accgccagat
    17101 tttttttctg cacggcggga aggattggca ttttggtttc gtggcaggcg cgaggattca
    17161 ccaggcacga ggtgactttg ccaacgaaga tctgatcgag gcagccctgg ttgcagccaa
    17221 tacaagtgtt gatctcatcg gctcgccctg attgcgcttt tgacagcagt tccgcatccg
    17281 cgagaaacgg tcgcgccatc gatactatat cggcatcgcc gcgcgagaga atatcgtcgg
    17341 caacctgcgg atcgttaatc cggttggtgg ttaccagcgg cagcgagacg tggcctttca
    17401 gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg cgtggcgatg gtcggaatcc
    17461 gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc gcccgccgct tcaatggcct
    17521 gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc gactaggtcg agcatcgaca
    17581 accggtaaat aataatgaag tcgttgccga cgcgttcgcg caccgtacgc actacttcta
    17641 cggcaaatcg catccggttg cggtaatcgc cgccccactg gtcactacgc tgattggtgc
    17701 gcagcgtcag aaattcgttg atcaaatacc cttcagagcc catcacttcg acgccatcgt
    17761 atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc gtcgatcagt tgcaggatct
    17821 cttcatgggt gagttcgtga ggaacgaaac ggttgatggg ggcctgcaat gcggacggtg
    17881 cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa aatttgcagg gcgattttgc
    17941 cgccttcctg atgtacggct tcggtaatgg tgcgatggtg tgggatctgg ctggcgtcgt
    18001 tgagcattgc gccaccttcc atgccaacgc ctgttaaatc tggtgcaatg ccgccgctga
    18061 caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa cgcagccagc cgttcagcac
    18121 cgtccgggta ttcctccagc ccggtgtgca ttgaacccat caacacgcgg ttttttaacg
    18181 tggtaaaacc taaatccagc ggggcgaaca gcgacgggta gctcataaaa ttgtccagta
    18241 tgtaaaataa ttgttatgta gtcggatgag ttctaattta gccgtcgatg gagaaaaggg
    18301 aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa tacgcccggt ggtaacgccg
    18361 gatgcgacgc ttgccgtgtt ttatccggct tgggggattg cacatgtagg gcggataagg
    18421 cgttcacgcc gcatccgcca gccgttgcaa attactgctt acggaaaaaa tcgttataca
    18481 acatatacaa atgagcggca ctccaggaga aatttggtgc gccttgctgc gcgcctgtca
    18541 gcgggttgta attctcctga attgggccat cggcggttaa tcctttggcg tgcctgaaga
    18601 acgtatccgc cagcttcagg gcatcatcac gataaccgta acgctccatc cctttcagac
    18661 caaaccagaa ctgatccacc catacgcgcc cgcgccagta gatatcagcg ccaaaagccg
    18721 gattggttaa cgccgccgtt cccagcggaa caaatgtgtt gaactcttta ggatcgagca
    18781 tcaccttcac caccgcgtcg gcattggcct gcgttgccgc accgttaaac agcggcgacc
    18841 agccttccgg ccctttaccg cgctcaacaa tcggtttgcc cgcgcagccg ttcgccagtg
    18901 gtttatcttc aatacgtacg tcatagtaga actgggtagt cgggtcgaac atacaggtgt
    18961 tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg tttggcctct tctggcttac
    19021 cgaggatggt tgccatctcc gccagataat ggttatcgct gtacatatag ctggcctgat
    19081 ccaccgactc ctgcaatagc gagtagccca gcaacgttcc gtcctgactg cggttttcgg
    19141 cgaatttcac cgtccagtcg ctacgtttgc cgccattagc gacatattta tccaactgtt
    19201 ctttgtcgat aaaaccaaag acggcggcat catcacgccc cgattcccac gacgcagcga
    19261 cctgtgccgg aatttccaga ctgtcgtact ggcctttctc caccacgcgg gcgtagttgt
    19321 tcagcccaga ctgcgtctct tctttatcgc cttttttcac cgtaaacagc atctcgccgc
    19381 tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc cggcaccccg ttgccgttat
    19441 gatcgcggtt acgtaaccac cagtcgtgat aggccaccag tttcgggtac atctctgcca
    19501 accaggcttt atcctgggtg acgttgtata cttccatcac cgaccaggcg gcaaggctgg
    19561 gtttggtatt acgttcgttc cagttgccgc catcgccacc acgctcgggg ctaaggttcc
    19621 acgctatcag gtcggggaca aagcccacat cctgcggacg cacgctgtcg ccaggctgga
    19681 tctgccagga gaagaccgcg cggatattct ctttggcgat atccggattg aaatgcgcca
    19741 tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg attgccggag aaccagcgtc
    19801 cggtcaccga cggcgtgacg gtgttataat tcaccgcacc gcccggtgag cgccagttac
    19861 cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc gcatccggat
    19921 tggttaaccc tttcttcagg tattcttccc agcgttgctg cgaggcggtg agataaaacg
    19981 ccggacgcgc cagaatatcg cggatctgca tttgctcttt gctaacttcc tgagcggtca
    20041 gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt gatatgcgcc ttactggtaa
    20101 agcgattacc gttgatttca gtctgcaccg gcagggattt atgcacctga tattccgatt
    20161 cgccggaggt cagcagatcc caggtggcgc gcactttgcc aaaggtgact ttcaggccat
    20221 cacgggtggc gctgattttg cgctgatagt cagggtattc gccagcaatg gttttatcgg
    20281 aaagcggttt cccttctttc gcttccagtt tttccagcag ttcgccatcc cacaccagat
    20341 ccagcggttt attgctgatg attttagttt ccagtagtga cgtgcgcggc gtggcgaagc
    20401 gcagagtcat ttcgacctgc acatcttttg ctgtcagttt ttgcaccagc gcgccgggaa
    20461 tactgtatgc ctccagcgtg aagttgactt tcttgccgtc ttgccagaca gtcaggcggt
    20521 cgaaattgct ggccataaag ttgatgtatt cttccgtcag cagcgcaacg cccggaaagc
    20581 cgcccatggt gttcgggccg tctggcaaca gatgaccatg ccaggctccg agatcgaaaa
    20641 acggattaaa acgctggtga tcgtcgtaat cgtaatcctt catgtactgc ggcgcaccag
    20701 tacggttaat cacgttttta taattgtcgg cgttagcggc atgagcggaa aaacttatca
    20761 gcagagcgca ggttactggc gttaaaatag ttttgatttt catgagatta cagtccttat
    20821 taccagtaga aatattgcat caggaagatt tggtcgctgg gatcgttaga ggcatttttc
    20881 atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc cgccatattc gtaccagata
    20941 ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg tttggcgtag
    21001 gtgtaagcgg tggagagata aacgcctttt aacgcttcat acatggcgct caccatcatc
    21061 ccgctttcac ttcccggtac gtccagcccg tcggcacgtc cggtatggtg ccaggcgcga
    21121 gcggcaaagt taggggtaaa caggcccgtg agatagagtt gtcctgtgcc gtcggtaatt
    21181 tccagaccat tcatccaggt gatgccttcg gcaaggtcat actggatgta accgttcatc
    21241 cattgccgga taggtgtatt tgtcgtcgta gcgatcgtac ttgccaaaat gtagccaggc
    21301 tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa agttgccgct
    21361 taagttctgt accttcagcg cggtcataat gtcgcgggta ttgggaatga cgtagcccaa
    21421 atcgggtgtg aagtcgcccc accattgcag gtcatccagc gaggaatcct gacgcagact
    21481 gagcatgatt tcggtgccat cattggtacg ataaccgcca tagaaacggt taatcccacc
    21541 ttcgaaacca ccccagctat ggtcaggcac ctaggcatta ccgtcgtggt cggcctgcac
    21601 ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct ccaggccacc
    21661 ttcaatatag gtgccgtcgc tgtatttatg tttattctca ccctctaaat ccatatatcc
    21721 accaagccct aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcggg
    21781 tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta
    21841 gcaaaacggc agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgttaat
    21901 ccatgagcta aagaaaaagt acagcctttt atcgttgtaa ttataaaaat ataaaggcag
    21961 aaactatttt ccctctcctc atccaggaag agggaaaatg cctgctcatt ttttattagt
    22021 ctgcttaatc aacattgctg gctggcgtcg cttcaatatg gcgctcttca gccgttaatc
    22081 cctttatata tttgctgtat ttccaccacg caaagccgag gaagataaca ataccgccga
    22141 cgttataaaa aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc
    22201 ctacggcaaa tatcgcaatc agcattgaaa caacgataat accggtgcgg cgggagccca
    22261 tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa gccagcatga
    22321 tgaataacgg cggaagcatg gacgctgcgg cggtcatatt aataatggta ttcatcagat
    22381 cctgcacggt attggaaccg agcatcggga taatcatcag cgggatgacg atcagaaact
    22441 ggatccatgc tgcgcgcgcc ggaacgccgt tttcgttaag ttcgacggtt ttcttaccaa
    22501 agatgccttc cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc cacatcagca
    22561 gggaaccgaa cattgcggta aaggacacca gaccgacaaa gcgattcatc aacgcttccg
    22621 gcagaccaaa atacgccgcc atgccgtgga atacctgcac cgatccgccg gtaaatttca
    22681 actctttgct gctgacgaag acgttaatca gcaccgagga gacggaatac agtacgccga
    22741 taaaaatccc ggcgaggatg atcactttaa cgaacgattt cgaaccgcct ttgacgtcgt
    22801 taacgtacac cgcgacggac tccgcgccac ctgcggccat aaagatccag gtggtaacgc
    22861 cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac ggtgatcgga tcggcaggct
    22921 gtacgccgcc aaccagtgcc gtacctgcca gtaaaatgta ggagagcgtt aacagcagca
    22981 tcagcgttga agtgacggag gtaattggcc ccagcatttt cgccccgttg gtggaaaccc
    23041 aggtggagaa ggcgaacagc accatactga taacggtggt ggcaaccggc gtcataatat
    23101 attcgtagcc gaggaaggca tacgaagcat aagcaataac gcgcggcaac agcgaggtga
    23161 aaaagaacag atttacgaac cagtaagtgt aggcagtaat aaatgcccaa cgtccgccca
    23221 gcgaactttt tacccacgcg tagacaccgg cttctgagtt tttatttaac gaaacaaatt
    23281 ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa aacatcggtg
    23341 ccgaggccag tccaagctca atattattat tgataacgtt gttaaagctg taaacggcgg
    23401 caaaagtcag cgagagcaag ccgaatttgc cgattgtatt acgtttggta tcagacataa
    23461 cacatctccg aaaatgtgta gggtagcggt ggcggcaaac tccttttgcc gccgtagttg
    23521 ttatttgtta tggaaataac catcttcgat ggtgactttg agaaccactt ttttgaccgc
    23581 gttattgcag ataaaccgat acgcttcatg gatatcgcaa ataacgattt gcccttcgtg
    23641 gacctctacg gtttctccgc agccttttaa atattcacgg tcagtttcat cacgataata
    23701 ttccactacc tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc cttgcaggta
    23761 gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg cggagtcatt
    23821 ttccacacgg taggtcaatg aatcaccgat ggagtgggcg acgccaggct gaatgttgtc
    23881 gatattttca atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt aaatctggcg
    23941 gaactgttct aagttatcga tgatcctcat tgcttattct cgctgtgcaa attcgtggaa
    24001 aagaaccctg cgccgaactc atacgacgcc aggctttgcg cggtagcttc tccgccagaa
    24061 accggcagca acgtaaagcc gtagctgaag tcacggaacc agacgcgcca ggagtccagc
    24121 acctcgctgc cccaggagtt ggagccgagg ccaagtagct ggtggtcgag gttcagggtg
    24181 atgtcatcac tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt ttcctgggta
    24241 tagtgccagg cgctgaagtt aattgggcgc tgcggaacca ccagcagacc gttgccgtgg
    24301 cggttagtca gtgccgtcca gcggacatgc tgacggttgc cgttgttctg cgggaaggga
    24361 tagttctcga acatggcatc gacggtgctg cgccagatat cgatgatgtt agcctgctgg
    24421 ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg ccacctgatc gtattcgccg
    24481 ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc gtaacgctcg
    24541 ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc gccagatgta ggtacagcgc
    24601 atcccgaagt caaacaccgg cggtgcaata actgtgcggc tgatgatcaa cacttcacca
    24661 tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca tgatctgcaa atgattcggc
    24721 tgccacaacc cttcgtactc ctgcttgtgg ttgtcgatca tcggcttgaa gaagttgatc
    24781 tttggctcgc gggtcagcag cgactcgcca tttacctgcc aggatgtcgg tttgccactc
    24841 atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa acgatcgtct
    24901 tccagcgtca gcggacgcgc attatttggt gcgaaaggca ctggctgcgc ggtgttttcc
    24961 ttcagcggga actgataagt ggcgatagaa tgcccggctt cgctgtagcg ggtgcgggaa
    25021 tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg cagcgtgatc
    25081 tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg cgtcgcgagc
    25141 gtttcacctt cggcgcgcac ctctgcgtgc agggtgtagt catcaagcgt ggtaaaccac
    25201 agtttatttt cgactttcaa ctcgccgcga gtcagcgcgt ggatttttac cggcgcgata
    25261 acctgtttgt actctttcag gcccgatccc ggcgtctgat cggaatagat caaaccatca
    25321 agacagaagt tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca
    25381 ttgccgtggt cgtcctgtgc ctggatcccg tggtcgcacc actcccagac ataatgaccc
    25441 tgaatgcaat cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc
    25501 cccatcgcat gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac
    25561 tcattcatca gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg
    25621 cgatcttctt cgtaatgaac cagtcgcgtg tcatccagcg ctttcgccgc atggtacatc
    25681 gcgcggatgt tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga
    25741 tggtttttct gtgcgtggat atggcgaaca atgcgctcga cgtagacttt ttcccactgc
    25801 ggatcgtcgg taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg
    25861 gtttccgcca tcacaaacag gccgtagata tcacacagtt cgtaaaaacg cggatcgttc
    25921 gggtagtgag cggtacgcac ggagttgata ttgtgctgct tcatcaactg gagatctttc
    25981 tcgacgcgat ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg
    26041 ccgtgcagca tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca
    26101 cggaagccaa cgcgttgtgg caccacttcc agaacgttgc cgttggcgtc tttcagcgtc
    26161 atgaccagat ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta
    26221 aaagcaaagc tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc
    26281 accacgcgtt cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg
    26341 agattttcca gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg
    26401 gtacgcacag tgaaatcgtt aatatgcgtt aggtgttttc cgaccagata aacatcgcgg
    26461 aagatccccg ccgaccacca catatcctgg tcttccacgt aggtagagtc cgcccactgc
    26521 atcacgcgca cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct
    26581 gcggtcaggc gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa
    26641 taggtttcga cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg
    26701 gtgaaaatac gttgataggc accggttggg ttatcgctgg ggacaaacgg cacatcgatg
    26761 gggaacggaa aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg
    26821 gcggggacgg taatatgccc ccagtcagcc attaactcag aggtgaaggc ttctggtact
    26881 tgcagcggat ggtcaaaaaa gtggaaattc cactgactgc ttaagggcag aaacaggctg
    26941 ctggtttcgc gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca
    27001 cgcggcgcaa gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct
    27061 cccggtagag agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctgggggt
    27121 ttagtaaaat ttttactaaa aagcagcagg taaatgcatt tactttaact ggatcacgaa
    27181 aataaacggc agcactgtcc ggcaaaaatg attgccgaac agtgtgtaag gggatttaac
    27241 gggtcgtgcc gcgcagtttt aatttgctgg gaacgaagac tagcagcggc agcgcgcgac
    27301 catcgcgggc tttttcatac accaggttaa cgccctgact tcccatcatc tcggaatgga
    27361 tgcgcacggt ggagagcggc ggaaaggtaa atcgcgcggt ggggatatcg ttaacgctga
    27421 taagcgaaat atcttgtggg atgttcaggc cacgttcatg aattgcccgc agtacgccga
    27481 tagcaatgga atcggaagca acaaacagtg ccttcggata gtcttcccgc gccagcattt
    27541 gttttgccag ttcataaccc gacgaactgg aaaaaccgcc gcgccagata tcctcttcgc
    27601 gtaccacttg tttcagtcgg ccatattccg caaaggcgac ctcacgaata tccgccttgc
    27661 caggctcatc ttcaccgcca ataaaaccaa tacgattaac gccctggttg atatagaagt
    27721 cgatgatttc tttactgatg cgtgccagat cgatatccac cgcatcgtaa ccgctgccgg
    27781 gttcgtgaaa gtcgataaaa cagatattgt cggtcaacgc gctggcagcg gcgcgcaggg
    27841 cgggcgtggg tttgccgaca attaaaatac cggtgacgtt tttaatgtct ggtaatccgt
    27901 tgtgttcata acagttggtc agttcgatcg ccagcttttc gcactgagtt tcaatgccgt
    27961 ggcggatcgc cagatagtaa ggatcgttga tctccagctc ctgctggtag ctgtagatag
    28021 ccagaatatg gtgttggttg actgcgcctg tctgtagttt acgggcgcta ctggtcttgt
    28081 actccagctt ttcggcgatc tcgagaatgc gatgtttcgt ctcttctttc acattcaatg
    28141 tcggatcgtc atttaagacc ctggatactg tcgccaggga tacgccagct tcgattgcga
    28201 tgtcttttaa tgttgccatg tttacctttt ttactttccc tgatgtgcgc ccccattgta
    28261 gaggaagtgc ttatcgggtc acctgttttc agtaaataaa gcgcgtttct ttgacgctgt
    28321 aacggtaaca cagcggctta ggaatgtgat cacgcctgca ttctccattt ttagcctaca
    28381 ggattgagaa aaacttttct atagtttagt aaaattttac tcgaagggag gaagcaatgg
    28441 aaaccgtggc ttacgctgat tttgcacgtc tggaaatgcg cgtcggaaag attgtggaag
    28501 tgaaacgcca tgaaaacgcc gacaagctgt acatcgtaca ggttgatgtg gggaaaaaac
    28561 actgcaaacc gtgaccagcc tggtgccgta ctacagcgaa gaggagctga tggggaaaac
    28621 ggtggtggtc ttatgcaatc tgcaaaaagc gaaaatgcgt ggtgaaacgt cggaatgcat
    28681 gctgttgtgt gcggaaaccg atgacggcag tgaaagcgtg ttgttaacgc cggagcggat
    28741 gatgcctgcg ggcgtacgcg tcgtataaaa agatcggatg gcgacgtcgt atcgccatcc
    28801 gatttgatat tacgcttctt cgacacttac tcgcatggcc gccagcgcct tacgcgccgc
    28861 tttgatcacc agttcacact gttcaatggt cagtgtcagt ggcggttcaa tgcggatcgt
    28921 tttggcgtta ttgagcgttc cggccaccag tacgcgctgg cggaacatct cgctggcaaa
    28981 gttatagccg atttcgttat caacaaactc aatcgccatc aacatccctt taccacgcgc
    29041 ttcctgtacc agatcgggat attcccgcgc cagttgacgg aaaccgtcca gcaacatatc
    29101 gcctttttgc tcagcctgag ccggtaagtt ctgctccagc aacacattga tggtcgccag
    29161 cgccgccgca caggccagcg ggttgccgcc aaaggtggtg gtatgcagga atgggttgtc
    29221 gaacagaact gaaaacacct cttcagtggc gatggtcgcg ccaatcggca tcacgccgcc
    29281 gccgagggct tttgccagac acagaatgtc cggctgaacg ttctcatgtt cgcaggcaaa
    29341 catcttgccc gtgcgcccca tgcccgtttg tacttcatcg aggatcatca gtgcgccgaa
    29401 ctcatcgcac agcttacgca cggcggtgag atagcccggc ggcggcagaa ttacgccacc
    29461 ttcgccctga atcggttcga ggatcaccgc agccacatca tcaccggttt ttttgcactc
    29521 attaagagcc gtgcgcatgg cttcgatatt gccaaacggc acatgacgga accctggcag
    29581 cagtggcata aacggtttgc ggaaggtcga tttcgccgtg gccgacagcg cgccaagtga
    29641 tttaccgtgg aacacgccgc tggtggcaat aaaagtaaat ttgccgcgcg gcgactgata
    29701 agccttcgcc agcttcagcg ccgcttcaac ggactcggtg ccgctattac agaagaagct
    29761 gtatttcagt ttaccgggcg ttagcgcagc aagggttttc gccagcatag cccgtaacgg
    29821 atcgagtagc tcctggctgt gcagtggttg tttcgcaagt tgattctgta cggcggaaac
    29881 cacaactgga ttacggtgcc ccacgttgaa aattccaaaa cctcccaggc agtcgataaa
    29941 ctcctgtccc tgggtgtcga caagcgtatt taaacctccc gcttgccact ctacggctcc
    30001 gtaatccccg ccggcggtaa cagatttgcg atactctaaa aaccccggat tgacatgctc
    30061 tttgaagtat tcaatcacct ctcggttaag tgctttcatc tcctcatgat ccagcgttcg
    30121 cttctcaatg agattcaggg cgtgggcgct gcacgctaaa gccgatgcgc tcgaaggtaa
    30181 cctgttcaaa atatgctccg gaggctcgcg tatcacatga taccgattta agtattgcag
    30241 ggattgcgcc actccggctg cgatcgcgaa aatcgggata aacacaaatg cggttatcat
    30301 ttgtgcaata tttaatgcta tccgttaaca tttgttgtta ttttgcacgg tttttgtcgg
    30361 gtatggattt gatgattgct gcgcaaatgc acgttttcag ggcaataatt gcctgatttt
    30421 tgggcaagat aattaacgtt ttcgtatagt aacagagtga aagtatttta aatcaaagaa
    30481 ttagatttgt gctttaatcg caaattgcga tctaaatcaa attaatcggt taaagataac
    30541 cgcagcgggg ccgacataaa ctctgacaag aagttaacaa ccatataacc tgcacaggac
    30601 gcgaacatgt cttctcatcc gtatgtcacc cagcaaaata ccccgctggc ggacgatacc
    30661 actctgatgt ccactaccga tctgcaaagc tatatcactc atgctaatga cacttttgtg
    30721 caggtgagcg gctttacctt gcaagagtta caagggcagc cgcacaacat ggtgcgtcac
    30781 ccggatatgc caaaagcggc gtttgcggat atgtggttca ccctgaaaaa aggggagccc
    30841 tggagcggca tcgtgaaaaa tcgccgcaaa aatggcgacc attattgggt gcgggccaat
    30901 gcggtaccga tggtgcgcga gggaaaaatc agtggctata tgtcgattcg tacccgggcg
    30961 acggatgaag agattgcggc ggtggagccg ctgtacaaag cgttgaactc cggacgtacc
    31021 agtaagcgta ttcataaagg cctggtggtg cgtaaaggct ggctgggtaa actgccttca
    31081 ttaccgcttc gctggcggac gcgtggagtg atgaccctga tgtttatctt gctggcggcc
    31141 atgctttggt ttgttgctgc cccggtggtg acgtatttcc tctgtgtgtt agtggtattg
    31201 ttggcaagcg cttgttttga atggcagatt gtgcgcccga tagaaaatgt cgcccgtcag
    31261 gcactgaagg tggcgaccgg agagcgtaat agtgttgagc acctgaatcg cagcgatgag
    31321 ctggggctga cattacgcgc ggtagggcag cttggcctga tgtgccgttg gctaattaac
    31381 gatgtctcaa gccaggtgtc cagtgtcaga aatggcagtg agacgctggc gaaaggcacc
    31441 gatgaactga acgaacatac ccagcagaca gttgataacg ttcagcaaac ggtggcgacc
    31501 atgaaccaaa tggcggcgtc ggtgaaacag aactctgcca cggcgtcggc tgccgataaa
    31561 ctttctatca ccgccagtaa tgcggcagtg cagggtgggg aggcgatgac cacggtgatc
    31621 aagacaatgg acgatatcgc cgacagtacc tagcgcattg gcaccattac ttcgctgatt
    31681 aacgatattg cgtttcagac caatattctg gccctgaatg cggcggtgga agcggcgcgt
    31741 gccggcgaac agggcaaagg ttttgcagtg gtggcagggg aagtgcgtca tttagccagc
    31801 cgcagcgcta atgctgccaa cgatattcgc aagctgattg atgccagtgc tgataaggtg
    31861 caatccggtt cgcagcaggt acacgccgcc ggacggacga tggaagatat tgtggcacag
    31921 gtgaaaaacg tcacccagtt gatcgcccag attagccatt caacgctgga acaggccgat
    31981 gggctttcca gcctgacccg tgcagtggat gagcttaacc tgatcaccca gaaaaatgcc
    32041 gagctggtgg aagagagtgc gcaggtgtcg gcgatggtga aacaccgcgc cagccgactg
    32101 gaagacgcgg tgacggtgct gcattaatcg gtatgccgga tccggcggtt ggagcacaat
    32161 gcctgatgcg atgctggcgc atcttatcag gcctgcgggg tgtgtagcgg gtgtaggccg
    32221 gataaggcgc gtcaagcgtc gcatccggca attgcaccgc gccactggcg gatgcggcga
    32281 ttaatccagt tgcgtaatat caaaagcggc gcggtcgatc accgcaatga tcttttttat
    32341 ttgtgcatca ctgatatcgc tctggttgac gcgtaaatcc agcaccgctt taaaattatc
    32401 cagcgcccgc ttcatttgcg ggttctggcg cagcgccgcg ccaacgcaac gcgctttgat
    32461 gcgttcttca atcatctcca cttgttcgcg gttttcttcc agccactgcg cgccttgttc
    32521 ggtcagcgca atctgcttct taccaccttc ctcttcgcgg atggtaatca gcgactgctc
    32581 ctgcagaaaa tccagcgtcg ggtagatgac gcccgggctt ggggtgtaat tcccctgggt
    32641 tagattctca atcgctttaa tcaattcgta accgtggctg tcatcgcgcg agagaatatc
    32701 cagaatcacc agacgcaatt caccgtgacc aaagaaacgt tgccgacgac cgccgccgcg
    32761 accgtggcga ccaccgcaac attgaccgtg ttcatgctgg tgaccgtgtc cgcagtgctc
    32821 gtgttctgac ttctcacctt tgcagcagcc ctcatggcgt ggctggcctt catgtttaca
    32881 acacccttcg tgatgatggc tcatatcagc ctccttttgc ttgattagat atatctaatt
    32941 tatatctgat gtgagcatta ctgcaagtac aaaaatgatt tttaaacgtg gaagcacctc
    33001 acatctaggc cggataagac gttcacgccg catccggcag cattgctatg gcactgaatg
    33061 ccttcacata cttcagacat atcagttcta caaatcgctt gcatttatca tgattaacaa
    33121 tcattatcat ttgcgaattt tatttagata tatctgatta ccatcacgaa ggcgataaca
    33181 atgaataaca ccccccgcta cccgcagcgc gttcgcaatg atctgcgctt ccgtgaactg
    33241 actgtgctac gcgttgagcg catcagcgcc ggttttcagc gcattgtcct tggcggcgag
    33301 gcgttggacg gttttacatc gcgcggcttt gacgatcaca gcaaactctt ctttcctcaa
    33361 cctgacgctc actttgtgcc gccaacggta acggaagaga gcatcgtctg gccggaagga
    33421 ccacgcccac cgtcgcgtga ctatacgcca ctgtatgacg aactacgcca tgaactggcg
    33481 atcgatttct ttattcacga cggtggggtc gccagcggct gggcgataaa cttacggtgg
    33541 caggtccgcg cggttcgctg gtggtgccgg aagattacgc gtatcagctg tatgtctgcg
    33601 atgaatccgg aatgcccgca ctgcgccgcc gcctggaaac gttgagcaaa cttgccgtta
    33661 aaccgcaagt tagtgcgctg gttagcgtgc gggataacgc ctgtcaggat tatctcgcgc
    33721 accttgatgg ttttaatatc gaatggctgg cacatgatga gcaggcggta gatgcgcgtc
    33781 tggcgcagat gcaaatccct gccgatgatt acttcatctg gataaccggc gaaggtaaag
    33841 tcgttaagaa tttaagccgc cgctttgaag cggaacagta tgacccacag cgggtacgtg
    33901 cagcggctta ctggcacgca aagtaactga tatataaaaa gaaaaaggct gacgatttct
    33961 cgtcagcctt tgctatatct ggtggcccct gctggacttg aaccagcgac caagcgatta
    34021 tgagtcgcct gctctaacca ctgagctaag gggccatggt agcggattat aaagtaactc
    34081 cgtgccgcaa tccagccatt accgcgcgcc tgctgttttt ataaacaatg tattttcaat
    34141 ccgttatact tttcctggtg atgttagaaa ggagtaaata tggttgagga tattttggct
    34201 ccagggttac gggtcgtgtt ttgcggtatc aaccctgggc tttcatccgc cgggactggt
    34261 tttccctttg ctcatccggc aaatcgcttc tggaaggtga tatatcaggc cgggtttacc
    34321 gaccgtcagt tgaagccgca ggaggcacag catctgctgg attatcgttg tggcatcacc
    34381 aa
//
LOCUS       DAJTRM010000036        33871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRM010000036 DAJTRM010000000
VERSION     DAJTRM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.036"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..248)
                     /locus_tag="OO322_001829"
     CDS             complement(<1..248)
                     /locus_tag="OO322_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5906736.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            864..1001
                     /locus_tag="OO322_001830"
     CDS             864..1001
                     /locus_tag="OO322_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906737.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1601..2380
                     /gene="ipaJ"
                     /locus_tag="OO322_001831"
     CDS             1601..2380
                     /gene="ipaJ"
                     /locus_tag="OO322_001831"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5906738.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3327..4256)
                     /gene="virB"
                     /locus_tag="OO322_001832"
     CDS             complement(3327..4256)
                     /gene="virB"
                     /locus_tag="OO322_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5906739.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4473..4709)
                     /locus_tag="OO322_001833"
     CDS             complement(4473..4709)
                     /locus_tag="OO322_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5906740.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4713..6614)
                     /gene="ipaA"
                     /locus_tag="OO322_001834"
     CDS             complement(4713..6614)
                     /gene="ipaA"
                     /locus_tag="OO322_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5906741.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6623..7621)
                     /gene="ipaD"
                     /locus_tag="OO322_001835"
     CDS             complement(6623..7621)
                     /gene="ipaD"
                     /locus_tag="OO322_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5906742.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7672..8763)
                     /gene="ipaC"
                     /locus_tag="OO322_001836"
     CDS             complement(7672..8763)
                     /gene="ipaC"
                     /locus_tag="OO322_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5906743.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8783..10525)
                     /gene="ipaB"
                     /locus_tag="OO322_001837"
     CDS             complement(8783..10525)
                     /gene="ipaB"
                     /locus_tag="OO322_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5906744.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10531..10998)
                     /gene="ipgC"
                     /locus_tag="OO322_001838"
     CDS             complement(10531..10998)
                     /gene="ipgC"
                     /locus_tag="OO322_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5906745.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11055..11681)
                     /gene="ipgB1"
                     /locus_tag="OO322_001839"
     CDS             complement(11055..11681)
                     /gene="ipgB1"
                     /locus_tag="OO322_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5906746.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11697..12086)
                     /gene="ipgA"
                     /locus_tag="OO322_001840"
     CDS             complement(11697..12086)
                     /gene="ipgA"
                     /locus_tag="OO322_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5906747.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12099..13583)
                     /gene="icsB"
                     /locus_tag="OO322_001841"
     CDS             complement(12099..13583)
                     /gene="icsB"
                     /locus_tag="OO322_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5906748.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13897..15513
                     /locus_tag="OO322_001842"
     CDS             13897..15513
                     /locus_tag="OO322_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5906749.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15523..15885
                     /gene="ipgE"
                     /locus_tag="OO322_001843"
     CDS             15523..15885
                     /gene="ipgE"
                     /locus_tag="OO322_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5906750.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15885..16343
                     /gene="ipgF"
                     /locus_tag="OO322_001844"
     CDS             15885..16343
                     /gene="ipgF"
                     /locus_tag="OO322_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5906751.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16352..17467
                     /gene="mxiG"
                     /locus_tag="OO322_001845"
     CDS             16352..17467
                     /gene="mxiG"
                     /locus_tag="OO322_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5906752.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17475..17726
                     /gene="mxiH"
                     /locus_tag="OO322_001846"
     CDS             17475..17726
                     /gene="mxiH"
                     /locus_tag="OO322_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5906753.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17739..18032
                     /gene="mxiI"
                     /locus_tag="OO322_001847"
     CDS             17739..18032
                     /gene="mxiI"
                     /locus_tag="OO322_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5906754.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18038..18763
                     /gene="mxiJ"
                     /locus_tag="OO322_001848"
     CDS             18038..18763
                     /gene="mxiJ"
                     /locus_tag="OO322_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5906755.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18760..19287
                     /locus_tag="OO322_001849"
     CDS             18760..19287
                     /locus_tag="OO322_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5906756.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19229..19924
                     /gene="mxiN"
                     /locus_tag="OO322_001850"
     CDS             19229..19924
                     /gene="mxiN"
                     /locus_tag="OO322_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5906757.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19917..20324
                     /gene="mxiM"
                     /locus_tag="OO322_001851"
     CDS             19917..20324
                     /gene="mxiM"
                     /locus_tag="OO322_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5906758.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20308..20736
                     /gene="mxiM"
                     /locus_tag="OO322_001852"
     CDS             20308..20736
                     /gene="mxiM"
                     /locus_tag="OO322_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5906759.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20866..21498
                     /gene="mxiE"
                     /locus_tag="OO322_001853"
     CDS             20866..21498
                     /gene="mxiE"
                     /locus_tag="OO322_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5906760.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21485..23185
                     /gene="mxiD"
                     /locus_tag="OO322_001854"
     CDS             21485..23185
                     /gene="mxiD"
                     /locus_tag="OO322_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5906761.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23204..24271
                     /gene="mxiC"
                     /locus_tag="OO322_001855"
     CDS             23204..24271
                     /gene="mxiC"
                     /locus_tag="OO322_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5906762.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24284..26344
                     /gene="mxiA"
                     /locus_tag="OO322_001856"
                     /gene_synonym="virH"
     CDS             24284..26344
                     /gene="mxiA"
                     /locus_tag="OO322_001856"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5906763.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26357..26758
                     /gene="spa15"
                     /locus_tag="OO322_001857"
                     /gene_synonym="spaK"
     CDS             26357..26758
                     /gene="spa15"
                     /locus_tag="OO322_001857"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5906764.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26762..28054
                     /gene="spaL"
                     /locus_tag="OO322_001858"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26762..28054
                     /gene="spaL"
                     /locus_tag="OO322_001858"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5906765.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28147..28485
                     /gene="spa13"
                     /locus_tag="OO322_001859"
                     /gene_synonym="spaM"
     CDS             28147..28485
                     /gene="spa13"
                     /locus_tag="OO322_001859"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5906766.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28472..29350
                     /gene="spa32"
                     /locus_tag="OO322_001860"
     CDS             28472..29350
                     /gene="spa32"
                     /locus_tag="OO322_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5906767.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29344..30225
                     /gene="spa33"
                     /locus_tag="OO322_001861"
                     /gene_synonym="spaO"
     CDS             29344..30225
                     /gene="spa33"
                     /locus_tag="OO322_001861"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5906768.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30230..30880
                     /gene="spa24"
                     /locus_tag="OO322_001862"
                     /gene_synonym="spaP"
     CDS             30230..30880
                     /gene="spa24"
                     /locus_tag="OO322_001862"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5906769.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30895..31155
                     /gene="spaQ"
                     /locus_tag="OO322_001863"
     CDS             30895..31155
                     /gene="spaQ"
                     /locus_tag="OO322_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5906770.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31155..31925
                     /gene="spa29"
                     /locus_tag="OO322_001864"
                     /gene_synonym="spaR"
     CDS             31155..31925
                     /gene="spa29"
                     /locus_tag="OO322_001864"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5906771.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31932..32960
                     /gene="spa40"
                     /locus_tag="OO322_001865"
                     /gene_synonym="spaS"
     CDS             31932..32960
                     /gene="spa40"
                     /locus_tag="OO322_001865"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5906772.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32973..33215
                     /locus_tag="OO322_001866"
     CDS             32973..33215
                     /locus_tag="OO322_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906773.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10542 a         4948 c         6802 g        11579 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgtccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacacttgtt gactacctgt gcggcatctg
      361 gtatcaaggt ggcgtgaagc cggttcaggt tctcatgcgg aacgttggaa cctatatccc
      421 cccgatacca aaggcgaaat gtaagtgaca aaatgcaatg aacacgttct ccgttaactg
      481 aggagcatgg tgataaataa aggtaaatgc agaaggtgta gagtctcgca gatttctgac
      541 atcatagagc ctaacattag atgcataacg ctacagattg tagaatctct acaggtgaaa
      601 tatacactgg ttggcgttta tgtcgcaaaa catgtcattc aggttcatct caccaataag
      661 gatatgagtg aagtggagga taagtgagtc tgctgtcaga gttttctggt gtatgtcagt
      721 aactgcgaac catgtctgct tgatatggga gcttatggag gctctctgta caaggttagg
      781 gagctaacaa agcagggata aaaagtccgc ctgatgcagg cacggttcgt taaatctttt
      841 gccattggta acaaaatggt gcgatgaatg ttcaggctat ctttatcttg atggtggttc
      901 agctgtttgg taaagaaatc gctgtaacaa tagaagaact gcaggcagta ctggtcccac
      961 atcgtttgct cagtcaactg gtgaagttcc gtagcgcgta aattaatgct ttgcatgaaa
     1021 cgttactgga gtggatgaaa gaatacctgc atatatcatt actgcgttgg attaccagta
     1081 caacagattg aatgaattgg acatgttgat agacggcatt gaaaagcagc ttataagcat
     1141 agtgcggcag agttaattgg agagcacttc gccttctgag tattaagtaa gcatggtggt
     1201 acctgcggat actatctatt cactgtgctc ggtcggcagc cttactgacc aaggagttgg
     1261 gggagttgat aacaaagttg aaaaaacagc tctcaaccaa ggtggttatt gtaacaatag
     1321 caaataagct ggtgtgacct gtatcacaat aaccgattat ggacgtaaat acgacaaaaa
     1381 tatgtcagta tcaggcggta ttaattgctg atgctgatgg acattaactc ttaataaaag
     1441 ggtggatgct gaaaggttgt tatggtgacg gacgtgatga cgacggaaaa gagtatgagg
     1501 ttttggattt aaagttcgtt cggggaataa ggacccggtc agctaattct catagtagtt
     1561 cgcattgctg gatgcaatac tgtgctgcaa ttactcgtcg atgtcggaac aacggaagcc
     1621 ttgcaaacgg ggatgtattc atacaggtgt tatgttatat ggggttttat tgcagggggc
     1681 tataccaaga gaatatatga tatctcatca gactgatgtg agggtaaatg agaatagagt
     1741 aaacgaacaa ggttgttttt tggcgaggaa gcagatgtat gacaattctt gtggagctgc
     1801 cagtttactc tgtgctgcaa aggaattagg ggttgataaa ataccacaat acaaagggtc
     1861 tatgtccgaa atgacaagga agagttctct tgatttggat aatcgttgtg agcgggattt
     1921 atacttaata actagtggta attataatcc gagaatacac aaagataata tcgcggatgc
     1981 cggttattct atgcctgata agatagtcat ggctacgagg cttcttggat tgaatgctta
     2041 tgtcgttgaa gaaagtaaca ttttttcgca agtaattagt tttatttatc ccgatgcaag
     2101 ggacttatta attggcatgg gatgtaatat agtacatcaa cgagatgtgt tatcttctaa
     2161 ccaaagagtt ttggaagctg ttgctgtatc ctttattgga gttcctgtcg gcctccactg
     2221 ggtgttgtgt agacctgatg gaagctatat ggatccagct gtaggagaaa attattcttg
     2281 tttttcaact atggaattag gtgcaagaag aagtaattct aactttatag gatatactaa
     2341 aataggtatt tccatagtta taactaatga ggctttgtaa attgtcgttt tttatctgtg
     2401 aaggagcttg aatattttgg ggtggggggc ataacaaatg atattactga gtacaaattt
     2461 agctatattg ggcggattag ttgtgtggga agacgaaatg tttctcttga aataagtgaa
     2521 ctccctcaag gaatgattgt gtgcttaaat atttaagtgc tggtaagttt gagaaggctg
     2581 acatattttg cagagcaaac agtgattgac ttggatgata tttttatttt agtcactcgt
     2641 tgtaattgga gtatgcttta ttttatttga aaatagatgt attttatacc cgcaatttaa
     2701 actgtaagcc ctatgagggg tatggatatt ataagttact ctttgaccaa gcataacttt
     2761 tggggttaga aaaataaatc tcttgttaat aattaatatg attttaaaat caaacttgtt
     2821 tataattata aaataatgta agtcggtcat tttatgggaa gattaggaga ataaaacagt
     2881 aattatattt gagaaatctg ctaatatatt cttaaagctt ttttgatact gtgaacgaag
     2941 tctgttaatg gtggattttt tgtttatcac catgtaaaaa aaattgagta gtaaaagcgt
     3001 tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa tattttcggc
     3061 cagtcactca tattcacata aaattttgac catcatgccg catcccttag ttgcacaatt
     3121 cattttcaat gatatgaata ttactaattt caccagataa ttttctcgtg gtcagcaggg
     3181 tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat aacagatagc
     3241 cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat tatctgaatt
     3301 gggcagttta catcagtgtt cgatgtttat gaagacgata gatggcgaga aattatatcc
     3361 cgaatagctt catctatttc tctctgaact gttttgttta ttttagataa tgtaaatatt
     3421 agagttctgt ttgttttatt ctcttttctt tttatgtaag tccgtttgtc tttagaaata
     3481 aataacgagt caacttttag cgaagggttt tgctttctgt ttttgctttt ctttatgatg
     3541 tttaaaattt cttttttata tatgtccggg ggcaaatttg tatcaaggtc ttttatttct
     3601 tcctttaata ttggtagtgt agaactaaga gattcattag ccttttcaag tcctttataa
     3661 taattgaata atatcttgta gtcattaaag ttaagttctg acgcgattgg aaatagagat
     3721 attatttcct gtggaacgct tgctgcctga aaggcacgag tgactttcgc gcgagacaga
     3781 ttctcttttt tggctatgtc tttataggac atccctgata ctttcagaaa attaagacca
     3841 ataccaagtt ctcggatgct atgctctttt gctgtttgta tatcgtttgc tagttttctg
     3901 gcatcaagag tagatatata ttcttttgaa tatagaactt caagatctgc tcctgcatat
     3961 attgcagatg ctcttctacg agtgccatcc agaatttcaa ttctaccatc aatctccctt
     4021 cctattacag ggaagaattg ttgtagcttt atagttttta tgatatcggc taatgattct
     4081 tctgttaggg attcttgatc gcgtacattc gttttttggt tgacgaaggt taaatcttgg
     4141 atccttttat gaggaatctt ggctttgata aaggaaactt tattaccaga atggagtgta
     4201 aactctttct tttggccttc ctttatactt aacaagtcgt tgcacaaatc caccatatca
     4261 caccctgttt attcatattg atttatattg acaaaagtta aatgcagtgt gagcaagatt
     4321 gtatgtaact gttgatgata gtcaacggta gtcttaaaaa ttactatttt aatagctgga
     4381 ttgatgcaat ttatctttat tggataaaag aataatattc ttgtggagct ctgatgtgag
     4441 agctccatag atgaaaaaat gcttatcaat tactatattg attttgtact atttttgact
     4501 acagaaataa gatctccaat agtaataatg tgttctaatg tagattcatc tatacgtaga
     4561 ttgaactcaa aactaatcat atccagcatt tcaatcagag aaagagaatc ggcagaaaga
     4621 tcttcaataa atttagtttc ttcagatagt ttgctgtacg ctataccata gcaaaaggcc
     4681 actattgata atattttttc ttttatcata ttttaatcct tattgatatt ctttaatact
     4741 tttgataggg aagtggttac atcttttgct gctttatata tattattgtt ttcagctgta
     4801 atatcattgt tattttttaa atcagatata tcatctgtaa gtaattctgc agaggtatcg
     4861 tcaatttttg acaatacctt gctgagggcg ctagatactt cttttgcctt ttcatagatc
     4921 gcatgatttt tatcaattgt atctgcgttg gtttctgtta cgtagtttct tatatttgca
     4981 tctgtaactt ttaatttgtt gtttattcta tgttctgttg tggattttgt aatgtccttt
     5041 gatttttcct gtaactctcg ttcttgtgtt cctgatttag ttccaatgag agatataaga
     5101 ttacttaggg agtttgttac tttttttgaa gcttcaaata tcgtttcccg tgttaaatat
     5161 gtgtctccat ttcttgataa aaaatcagtc tgaagtactt ttgaatcagt gtttgagttg
     5221 aatttattga tttgcgaagc tactctatga gaaagagaag cgctactttt agttgtatct
     5281 gatgagttat cattactgct tgtcactcct tccgggtgga gagggggggt ggttgaagag
     5341 ttctgtatgt tcagaggcct gtgtccccga gaaagagagt tatttgcaga tctttcttga
     5401 ctactcgtag ttaacaggtc tgaacctatt ccatccgaat tatctggagt atggttgaaa
     5461 gccttttcta aagctgaatt atttgcaaat gtctggacag tatctgatat agagtgtggt
     5521 agaacggaag ttactttttg aactagtgct ctttgtggat ttagtaatga gtttccgtta
     5581 cgggataata gatcgtttgt ctgagaagta ggagactgtg catcattttc tgggttctcg
     5641 tgatagctat tgtcatatac tctgttgtca aaaactctat tatcgtaagt tctattatca
     5701 ttatttatat gatagtgaat tacaggttgg gatgttttct cattggctcc gggggttggt
     5761 ccgccatctg gcatatcttc gggaggtgta gggaggccat cttggatagg atgcgaatct
     5821 tttatatctt ctaaaatatc attaaggact tttctcaaca gctcaggaaa cacggctact
     5881 gtatttaagt ctcttgacag tttacctata ccaaatcctt tactatcaat agtattttta
     5941 cgttgattgc gcaaactatc aaaggcaagt tttgcctgtg cgtcaactaa atcatctagt
     6001 ttgagagatg aaacagtttc attacttaac tttaattcag caagaatatt attcttgata
     6061 aatgatgata cttcagtatt gaggagtttt tgtcccaatg ataaagcttc accagatgtg
     6121 tgttgttgtg ctaatagtgc aagtctgtgc tcaatgtttt gtcgtaattg atggagtttc
     6181 ttattctttg ccgaccatgg tccaaaggca gaatcttggg ataatttatc ttttataatt
     6241 agttccgaat actgtgcgac aattttttga agtccctttt cgttttcaaa attaagacca
     6301 ttttgatgta ctaaggagta caaaacattt gaaaattcga gaatctcgct tttggcatac
     6361 gcattcattg agttggaata aatgcttctt aacacatctt ctttccctga ggaagaaaat
     6421 aaaaaatcaa gacatttctg attaaaagac gttgcaaagt tttctttttc agacactgat
     6481 gctggtgttt ttagagaagt ttgcgaacta tggatatcgg atattttgct ttttaactcg
     6541 ctgtattctg ttgatgatgg tgaagttgcc ttatataaga atgttggcgc ttgagtatta
     6601 tttacattat gcatggcgca cctcagaaat ggagaaaaag tttatctgta tctgtacatg
     6661 agcttattgt actactcaaa acctttacta aattatcaaa aatactattg gcattactgt
     6721 atttttgaac taaagtttga agattatttt tcattgtttc atcttcggca gagaatccgg
     6781 cattccatgc ctgatatttt gcattatcta gcacaacctc gccatttcca cctagattat
     6841 ctaagctttt taacatattg tctattgggg tcatgtttat actgacaaca tatcccccgt
     6901 ttttttgaga taccttgccg attgttccac ctaattctgt aagccattta tttgcttgtt
     6961 cctgactaac agtattattt gctggatata gcggtttatc tttatatttt tccttgagtt
     7021 cttccaatgc ctttttaagc gagttgactt gtaatttcac ggagtttccg tcgttacctc
     7081 cgggagagat ccagccggca agactggaaa gaacagcgct aaaatcttga tacatttgag
     7141 tatatgaact aacggcatgt tcatatactt tcagatactg ttcattaata tcattgatgg
     7201 agtttgcaat tttagcccac agttctctat gagatatcat ttgatctcca tcaagctcgg
     7261 cttctttcgg agctgaatgt aataattctc ttgcgtcttt attaattgga tattcgtgcc
     7321 tggaaagaat atctaatagt tgagcggatt tatttacatc catgctaatc tgagatgaat
     7381 gtaatgctat ttcttctagc gatgttttag tcaacgtttt ttgtgaaagc tctttcttta
     7441 atgcctgatt tgttgttctg atattatgaa gggtgtcgtt gagcatagta agggaactta
     7501 caggatgaga actggtcgtt gtttttatat cagaattaac tgtttcggtt gatgaaccgt
     7561 tggtattgtt tggactgaat gatgaggtgg aaatactatt agtcagagtt gttatattca
     7621 tggttatttc cttacaggaa gagccatata ttattatggc tcttggatat attaagctcg
     7681 aatgttacca gcaatctgac tggctgtcga attctttgat tggttgatgc tgtcaattat
     7741 attcaataat ttttgtatta attgatttgt cgcttgggat gcttctttag atacttggga
     7801 tgcttcctct gcttgtttac tgctggcctg actgattagt tgttcttctt cttcaagagc
     7861 agatgcataa cgccctcctg atgtggatat attaccagca acggctgatg tttccattgt
     7921 tgcacggcca atgttttgtt tttgctgcgc agaaagggta ttgagctcgt aagttcttct
     7981 ctgggtgtca attttatcct gcaaagaaat atcgggagaa ctaagagaag ttttatgttc
     8041 agttgacagg gatatattat ctggcgccag tttattttta cctaaaaatt ttgtgctaga
     8101 gttagtttgt ggtgaggtga tatttgtatc tatttgttta tttaacccta atttagaacc
     8161 tgcaagctct ttttcaagag attgagcagt ggcaaggttc tttcttaagg ctcctttttg
     8221 gtcgctaatc cctgaatgcg tttttttggc accgataccc gttataccta cttgtgtgac
     8281 tgctccagta atgcttgatg atagggctgc caggccttgc cgaacaatgt tctctgcagc
     8341 tgattttgta gcatcgaacg caatcaatga caattgagag cccaatttag tttctgcagt
     8401 gcggagagca gaaagtagaa cggctacact aataattaaa gaaacagcat tagaagaaag
     8461 actggaaata tccagagtgt tctctggagt aagggtgtga ctcatctctg aaagatcttt
     8521 catctttttg tcagtaaggc gagcaatgat ttctgaatca tgctgaacct gctctgataa
     8581 ctttaaaagt tgatcatcat ttaatgtggt tgttaatacg ggatttttac cgacattaag
     8641 tggaatatgc gctgcaatct gctgataatt ttgtgatttt tgtgtttcgg aagaactttg
     8701 agtttgtttt gtggatatat ctgtatataa aatctgggtt ggttttgtgt tttgaatttc
     8761 cataacattc tccttatttg tatcaagcag tagtttgttg caaaattgct tttgcaacat
     8821 cagttctatt agcctgagag ttggacattg aggctaatag atctgcaatt acttcctgca
     8881 attggccgaa tttttctatt gcttcactga tatattttga cagttgttca acttgatatt
     8941 tcgataatgt caggtctgct agatttgtcg acgcgctgtt ctggaaaaca gcagaagcga
     9001 cacttcctcc cgcttgtgtg gcagagttaa cactttctcc taataaaact gcttggttta
     9061 aatgggtgga aataatttgt ttggatatta cttcatcacc cgctgcacca agaaatttat
     9121 ttaatctggc aacagcatta gtgattaaat cgtccagttg agaagaaaaa ttcttgagaa
     9181 actttggtat aaggtctgtg agggttttac ctattatttt gccaatattt tctgcaagtt
     9241 ttgctgctgc ctgtttacca acagtggcta cgagaacgac tgctgcaact aggacaagag
     9301 cgcctgcgat tgcccccaga atagagccaa tcattttggc ttttttcgag tcgacgccca
     9361 agccttcgag catttttgta aatgcatctg aaaggagttt gattaagggt tcaatgactg
     9421 ctttcatgat cggattcagg gcttgttcca tgaaggaatt gccggtcgct gcttgtacta
     9481 tagcatccgt aaccataaga gctaaaccaa cagctgccag tgctagagag gctcctccag
     9541 aaaaagctgc tgcaacaaca ctaacgatag ttaaaagtgc cccaagtatt ttcccaacac
     9601 aacccattac tctgttgagt tcttctgctt tacgtacttc agcagcatac tcatcagatt
     9661 ttctctccat ttcagttttt cttgattctt ggagagactg gaatagagcc agatcatttt
     9721 ttaaagattc ttcattattt tttccaacta gttgaataaa ggttgccatc aattgagtaa
     9781 cactggcaag tccggttaat gatttctgct gggttgatag ctgttcagca gatgctgtgt
     9841 ttgaaaatgc agaaaaagag tctatttctt tttcgagttg cattgattta tctgtaagtt
     9901 tgctatgaat tgacagggtt ttctgctcaa tcaatgtcct gtctttaact gctgcgtctt
     9961 ttttgatagt gagttgtatt tcttcccggc ttaatttttt cttttctggt gactctgggt
    10021 cgagttcgga taatcttgtt tgaatttggt taattttatt ttctaggtct tttattttag
    10081 aatctgcgtt ttttagttta ttaatttgtt tttcatagtc tctggttagt ccttcagttt
    10141 cagatagaag agtgttaatt ttatcggaga attctaggtt tttttgctgt cttgcctgtt
    10201 gctgggactt ccaagcagta attttatttg ttaatgcagt taaagatttt tcaccgagta
    10261 tttgaataag gtttccaatt aaaagcgtta gttgggaact tgcatttaat gactttggtg
    10321 ctttaagttc agggattaat atatttgaag ttgagtgtgc attagttgta ttaatatttt
    10381 ggttagcagt aagatcagca attgtaagag aaaataattt gttagctgca tcatttgcag
    10441 cttggatagt attgtctcca agctcagtgg aagccaatat tttggcaaga ggaaaaccag
    10501 tggttgtggt gcttacatta tgcataataa ttactccttg atatcctgaa ttgcgtccaa
    10561 gtatgattgt gcttttattt ttaatttttc atcattgctg tgttgaatta cgagttcgaa
    10621 gcactcttta gcttttaagg gggctttcaa ccgaagctgg cattgtccag tatggaatac
    10681 tggtgtatag tcattttttc ctaatgcaaa agcgacagca taaaggtctg ctgcttgttg
    10741 gaactgttct tttatctgat aaatagctgc gagtcccata atgtagtcta cattgtaaaa
    10801 gtcgtatata cataaaaacc tgaagaaaac ttcagcttcc tctattcttc ctttgttgta
    10861 aaagtcataa gcatatgaat aaatgtcatc catcatatca tcaggaattg cattaatatc
    10921 tttcagtgta gcgccagagt taattgcatc aattactgca gtagagatgc tttcattttc
    10981 ggtgatattt aaagacataa ggtctccttt ttgtctgctt tactttaata agtataagat
    11041 ttaatataaa agatttaatt tgtattgctt tgacggtata cagcctttag aacctctgcg
    11101 cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta gacggaactg
    11161 gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc actgctatag
    11221 cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc tgtatgactg
    11281 atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt ttcaatagct
    11341 gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat ggccatttca
    11401 tctatacttc tgtttatcat tgaagagaca taagtggttc gctcttggct catccaacaa
    11461 aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc cctgatttta
    11521 tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa gttaaaaaca
    11581 gataatattt tctttacaag cttattgcga cttaacgaat caaatgatgg gcgaggaatc
    11641 gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc tatatgttag
    11701 ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa ttcatacaaa
    11761 gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt caaaacacat
    11821 tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa agagtagtta
    11881 aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg ttcattaaga
    11941 agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat ttgttcttcg
    12001 agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt tatttcataa
    12061 agtttatcat atagtttgcg acacatttct atggccttct atatattaga atgagagtta
    12121 ttcaataaaa gcttattgat ctgtaataag tcgtgaatgt aaagtttggt tccaagatct
    12181 ggcgatttaa gagaattgta ataatcaata atttttattg atttttcgat gatttcttct
    12241 tttttatctg ttgcttcggt ttttaattcg tttaatacac gagtcatatt gctgggtgct
    12301 gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc tgaagataca
    12361 ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt tggtgtgtgg
    12421 ataaggttaa ttttattgaa acttttaaga tagctgcgtc gtaattgagt caatctggaa
    12481 agatcaaaat cagattcaat atacttcatt atttcgttat aagactgatt gagactttca
    12541 atgcgttgcc taactttatt tgcatacgcg tatacatcgt taggtgtagc aaccaattta
    12601 acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt aagtgccata
    12661 ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct gaaagcatct
    12721 gctttctttt tgcatataaa ttttgacatg tttgtttcat caaggccaaa aaggttatgg
    12781 ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac tttatcagca
    12841 ctgacccccc aataaccata ggggtctttt ttttgattag ctctgggaaa aaatattttt
    12901 ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg gcctgcatca
    12961 agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgcttata ggtatcgaca
    13021 ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt aacattcccg
    13081 ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt ttttatactg
    13141 atactagtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa aactaaatgt
    13201 ctttcaagat ttttggctgt accgatgcca tgaaaacgct ctgcggtatt caacattaat
    13261 ttgatgtctc tacctgtgaa agagtgttga attgagtttt tagctgcttc ataggcggca
    13321 aacgcacttt ctgcagattt ttcgctagct aatgtattga ggaaaagcaa taaggatttt
    13381 tgattctcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt tatctttgca
    13441 ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg tgctatcaat
    13501 ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct tgcgtcaatg
    13561 aaattgctaa ttttgaggat catactttat taactctcca ttacttggtg attgtgggga
    13621 tgtaaaatgt atttggtata taatatattc aattaataaa tttagaaact tgagcctgtt
    13681 aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat atgtagtgct
    13741 cgtttcatca tgaaatccca caagataaag tgcctgatgt atcaggctcg gagtgttata
    13801 gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag gtacaaaact
    13861 gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa tttgggattg
    13921 catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac cggaaagcac
    13981 aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa taaggggatc
    14041 gaagcgctta atcgattata tttacagaac caaacaagct taacaggaaa aagccttttg
    14101 tttgcgcgtg acagagctga agttttttgc gaggcaataa agttggcagg tggtgatacg
    14161 tcaaaaatta aagccatgat ggaacgatta gatacctata aacttggtga agttaataaa
    14221 agacatatta atgagcttaa taaagtaata agtgaagaga taagagcaca gctaggcatt
    14281 aaaaataaaa aggaattgca aacgaaaata aaacaaatat ttactgatta tttaaataat
    14341 aagaattggg gaccggtcga caagaatatt agtcatcatg ggaaaaatta tggttttcaa
    14401 ttaactcctg cctctcatat gaagataggg aataaaaata tatttgtcaa agagtataat
    14461 ggaaaaggaa tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa tatgtggctt
    14521 tcaaaagtgg tggatgatga ggggaaggaa attttttcgg gtatcagaca tggagttata
    14581 tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg taataaagct
    14641 gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca ggctttgtct
    14701 ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac cagtttaacc
    14761 gggggagagg agagtatgtt aaaagatcag gtgaacgcat taaaagggtt aaactctaaa
    14821 agggggaagc caacgaaatt attaattcgg aatagtgatg gtcttctgaa agaagtaagc
    14881 gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct aaaaatgggc
    14941 ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt gttgggggat
    15001 aatttcctca aaaatggtgt gattggaggt tgggctgcag aagcaataga aaaaaatcca
    15061 ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt aaccaaaaag
    15121 ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagaatgac tctattggct
    15181 tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag aacaggtatg
    15241 caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca gttttctcaa
    15301 ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct aatgaatagt
    15361 ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt catgaaaaaa
    15421 ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc aaaaatatgg
    15481 aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga tttagcagat
    15541 gttatttgcc gagccttggg tatcccttta attgatatcg atgatcaagc aatcatgctt
    15601 gatgatgatg tgctaatata tattgaaaaa gaaggagatt caataaattt attatgccct
    15661 ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct aaattactca
    15721 gaaaagatat gcttagctac tgatgatgaa ggcggaaatc taattgcgcg attagatctt
    15781 accgggataa atgaattcga ggatgtatat gttaatacag aatattatat ttcgcgtgtg
    15841 cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc cgtttcgtat
    15901 ttattttgct gtgttttatc ccatatttag ggagagctga ttgttgggat aaggctggtg
    15961 aaaggtacaa tattccatca agtttgttaa aagcgattgc ggaaaaagaa tccggattta
    16021 ataaatctgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata atgcaaataa
    16081 atgactttca ttctaaaaga cttagagaaa tgggatattc tgaggaaatg ctaattagtc
    16141 atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg atgatgtatg
    16201 gaagagggtg ggaggctgtt ggagcctata atgcaggaac gtctccgaaa aagaaaaaag
    16261 aaagattaaa gtacgcagag gatatctata gacgctattt gcggatagca gcagagagca
    16321 agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact caaatcttgc
    16381 accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc cgttatatta
    16441 tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg gaaacgacaa
    16501 tttcccagaa aatattattc ctgtaacaga tagtaaatcg gatggtataa tttatttgac
    16561 cataagcaaa gataatatct gtcaatttag tgatgaaaaa ggtgagcaaa ttgatatcaa
    16621 ttcacaattt aattcatttg aatatgacgg gatttctttt caccttaaaa atatgaggga
    16681 ggataaatcg agaggccata tactaaacgg aatgtataag aaccactcgg tgtttttttt
    16741 ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga aagatgaagt
    16801 aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca ctggacagtg
    16861 caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcgtctgtag ccttaaataa
    16921 aaccggattt accaaatgca gatatattct ggtgtcaaac aaagaaataa atagaattca
    16981 acaatatatc aatcaacgat tcccatttat caatttgtat gtgttgaatt tagtgagtga
    17041 taaagcggaa ttactggtgt ttttaagtaa agaaagaaat agttcaaaag ataccgaact
    17101 tgataaattg aaaaatgctc tgattgttga atttccgtat atcaaaaata ttaaattcaa
    17161 ctatcttagc gatcataatg caagggggga tgctaaaggt attttcacaa aggtaaatgt
    17221 ccagtataaa gagatttgtg aaaataataa agttacatat tctgtcagag aagaactgac
    17281 agatgaaaag cttgagctca ttaatagact gatttctgaa cataaaaata tatatggaga
    17341 tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta aatcatatct
    17401 taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt tagataaaaa
    17461 taagtgagga taaaatgagt gttacagtac cgaatgatga ttggacattg agttcattat
    17521 ctgaaacttt tgatgatgga actcaaacat tacaaggtga actaacattg gcactagata
    17581 aattagctaa aaatccttcg aatccacagt tgctggctga ataccaaagt aaattatctg
    17641 aatatacatt atataggaac gcgcaatcca atacagtgaa agtgattaag gatgttgatg
    17701 ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt tatccagtca
    17761 atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca agtctggaag
    17821 acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa gtatcacaaa
    17881 taatggagat ggtaagcaat ccagaatcat taaacccaga atctttggcc aagttacaga
    17941 cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca agaaaaacag
    18001 tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata aaggttttat
    18061 tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa tttctaattt
    18121 atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata ttactgctag
    18181 aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga catttgcatc
    18241 ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg atatctcaca
    18301 aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc gtttatatag
    18361 tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta tttcggcaaa
    18421 aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga tgcatatatc
    18481 agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta atattaagcg
    18541 atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca tattaactcc
    18601 gaaagaagaa tatgtttata caaatgtaca acctgttaag gaagttaaat cggaattttt
    18661 aacaaatgaa gtaatatatt tatttctcgg gatggctgta ctagttgtca ttcttttggt
    18721 atgggcattc aaaacagggt ggttcaagag aaacaaaata tgataagaat ggatggaatt
    18781 tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag aaatcggttg
    18841 aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa taatctcata
    18901 attaataaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac cgctatgttt
    18961 gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca agagtctcgg
    19021 ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag taagcaaggg
    19081 gaggccgcta ttcgaagtgg tttttggcat cttattgcgt ggaaggaaaa tatatctgtt
    19141 ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg aaattatacg
    19201 cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa aaggcacttt
    19261 accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg aaaaggaatt
    19321 atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt gtgttataaa
    19381 ggaggctacg aaaaaagctg aatcactgcg tatagatgct gtatgtgatg gttatcaaat
    19441 tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga agttaaagca
    19501 aaatgaaaat agaagaaata ttgaggacta tataactagt ttgctttcag aaaatttaca
    19561 tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc gtaatacagt
    19621 aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa agttagaact
    19681 tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg aagggaataa
    19741 ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg taatatcagg
    19801 tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt tcaaagagtt
    19861 agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc ctgttaatga
    19921 ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa gaagttgtta
    19981 atttaaatga gagattatca tcttcgcaat catttgatga ggatataatc tatgaaataa
    20041 tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg cacaataaga
    20101 ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa agttcacttc
    20161 ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt tatgcatcat
    20221 tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat tcattaatta
    20281 gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa gttgaaaata
    20341 tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc atcatctaat
    20401 tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat tccaaatgat
    20461 ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa atgtattagt
    20521 ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa tgttaaggaa
    20581 gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt ttctatgctg
    20641 aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat agagaaggat
    20701 ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta aatataaagg
    20761 tctaaataca agtaatatgt tttacattta ctctagtgga catgaaccag ttaacgttga
    20821 gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga agggtttttt
    20881 tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta tcgctttaac
    20941 attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc tctagtagag
    21001 catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg ggatagttgt
    21061 aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc tgaccagaat
    21121 gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt tctttatctt
    21181 ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga gcattatggt
    21241 gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa agtcaaagaa
    21301 caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca caatcaaaca
    21361 atcacttcag ctgccatgaa taatgggtat gcatcaacat cccatttttc aaatgagata
    21421 aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt agtgaaaaaa
    21481 ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt taccattgat
    21541 agttaatgct aataatattg actctcattt attagaacaa aatgacatag cgaaatatgt
    21601 tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat taaattatcc
    21661 tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg atttatctaa
    21721 tccagaagaa atgcttgaaa aactgacatt acttgtggga ctcatatggt ataaagatgg
    21781 aaatgcatta tacatatatg actctggaga attaataagc aaagttattt tgctggagaa
    21841 tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg atcacagata
    21901 tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc cggcattggt
    21961 tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta ttgggacaga
    22021 taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca gaacttataa
    22081 tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac gtttattaaa
    22141 taatggtaag gctctgagta acagacaagc ccaaaatgat ccgatgccac cctttaatat
    22201 tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta ccaatagttc
    22261 tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat tagtcaaagg
    22321 taatgatcag caaatacaga ttattcgcga tattatcact cagttagata tagccaaacg
    22381 acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa ataaccttgg
    22441 tgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcgtcat ttaatatgag
    22501 ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag ttatggcatt
    22561 aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac aagagaatat
    22621 tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg agcgcaattc
    22681 ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct tttcatctcg
    22741 gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc agtcaaatta
    22801 caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca aaatcagtac
    22861 aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc atgaaacgaa
    22921 ctctaacgaa ataatatcta ttccttttct ctcctctatt cctgtaatcg gtaatgtgtt
    22981 caagtataaa acaagtaata tcagtaatat tgtaagggtt tttctgattc aaccccgtga
    23041 gataaaggag tcaagttatt ataatactgc agaatataaa agcctcatat ctgaaagaga
    23101 aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag acgaaaaatc
    23161 attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg atgttaaaaa
    23221 tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa attcagatga
    23281 tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca agtatattga
    23341 ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag accttgagaa
    23401 actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag aagatgaaat
    23461 taatcacaag atttttgatt taaagagaac gttaaaagat aaccttcctc tagatcggga
    23521 ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct tagcattaag
    23581 ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaattattaa ctaaaattat
    23641 taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt cagctataca
    23701 ggctaaatta tttggcaaca aaatgaaact tgaaccacag cttttgcgtg catgttatcg
    23761 tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc ttggtaattt
    23821 tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga ttgtagacat
    23881 ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat ctaaattaat
    23941 tgcaattaag atgattagaa cttcagacgt aatttttatg aagaaactgg aatcctcaag
    24001 cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat tatatatttt
    24061 tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagaagtat cacgagccag
    24121 tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa gtcctcatga
    24181 tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag agcttgtcac
    24241 aagtgcatac aagaaagagc tttctagata acaggagata aaagtgatcc agtcttttct
    24301 taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta tgatcattgc
    24361 tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc ttaatattgt
    24421 acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga gtttttctac
    24481 attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa taagtactag
    24541 tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg gccagtttgt
    24601 cattggtgat agtctggctg taggttttgt tattttttca atagtaactg ttgtgcagtt
    24661 cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct tttctcttga
    24721 cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa ttattgatgc
    24781 cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt atggttcatt
    24841 tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta ttatttttgt
    24901 caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtccc tatctggtgc
    24961 attatctact tataccatac taactattgg tgacggattg gtctctcaga ttcctgctct
    25021 gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata gcgataatat
    25081 gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg ttacatcagc
    25141 tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt tcctgatagc
    25201 agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa aaagtctgtc
    25261 tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc atgactcatc
    25321 tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt taattttatt
    25381 atttgccgaa aataagataa atgcaaatga tatggaaggt cttattgaaa ggataagaag
    25441 tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa caagcaatga
    25501 gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata gttttaatat
    25561 atattttgac aaagtgtgta ttacagatga aaatggagat atagatgctc taggtattcc
    25621 tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt catatacaga
    25681 aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt atcaccagtt
    25741 gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa caaaaaatat
    25801 gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc gacatgtgac
    25861 tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt ctgttcgcaa
    25921 tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag atgtcataac
    25981 attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa tagctgtttc
    26041 tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa gaaaagggat
    26101 aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg atgaggtaat
    26161 ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt tcgttctttt
    26221 ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat ttaagagtat
    26281 actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat taaagactat
    26341 ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag atagtctttt
    26401 cacgattggt tgtcctccat caattattac agatttagac agccattcag cgattaccat
    26461 ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg ttatgttatg
    26521 ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca tctgcttata acatattaaa
    26581 tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc atcgttctga
    26641 tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg ggattgtttt
    26701 tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgggg tcttataatc
    26761 aatgagctat acaaaattgc tcactcaatt atcttttcct aatagaatct cggggccaat
    26821 cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc aggctggaat
    26881 tgaaagtaat gaaattgttg caagagctca ggttgtagga tttcatgatg aaaaaacaat
    26941 attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta agcccactgc
    27001 ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca atcctttagg
    27061 ggaggttact gataagtttg ctgttacaga taacagtgaa attctttatc gacctgtaga
    27121 taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt taacaggtat
    27181 taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga tttttgcgtc
    27241 agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg gtgctgatat
    27301 atatgttatt gggttaattg gtgagcgagg tcgagaggtt actgaaacgg ttgattattt
    27361 gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg attactcttc
    27421 ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt ttaggactga
    27481 aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag cattacgtga
    27541 tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt cggtttttga
    27601 tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct ctattactgc
    27661 attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg aagaggtaag
    27721 atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag gacaatttcc
    27781 tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg atgaaaaaca
    27841 tcgtattatg gccgctgcat ttcgggagct actttctgaa atagaagagc taaggacaat
    27901 tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa tctacaacaa
    27961 aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta cttatgagca
    28021 gacaatggag cttattggtg aaacaattag ataaggtttt aaaaatcaaa gataaatatc
    28081 aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa ctctttgtat
    28141 cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga gaatgactta
    28201 gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa gatcgctata
    28261 gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg aagaattgaa
    28321 gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct tcataaagag
    28381 agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa acagttgata
    28441 cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa acctaaattt
    28501 tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata atattgatag
    28561 tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa ttgcatctaa
    28621 ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa tttatagtgg
    28681 cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata ttccagaatc
    28741 aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa gggaaaaaat
    28801 aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg cagacaagaa
    28861 cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat ctgataatag
    28921 aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata ttgctgacat
    28981 catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa tgaataaaaa
    29041 tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg aaaatgataa
    29101 aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg gggctggaca
    29161 ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag atcaatttgt
    29221 aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca gatttgatag
    29281 cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac agagtgaaga
    29341 agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt atatgcaaag
    29401 caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg cagtgaaagt
    29461 ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac attatctttt
    29521 ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt actgcattta
    29581 tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact gtctgataag
    29641 attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca tttatgctgt
    29701 ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg taatcaagtt
    29761 tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa tgtaaggctg
    29821 gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact ttataattta
    29881 ttattgtgta atcaagttat tattggggat tatattgtga atgataataa tgaggcaaaa
    29941 attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt ggcattattc
    30001 aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa tatgacaatc
    30061 aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga catagttaaa
    30121 catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt ttctattgag
    30181 gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca tgctgagtga
    30241 catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag ttgctgctgg
    30301 tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac ttggtttgca
    30361 gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt tgttcgtgat
    30421 gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa aatttgatac
    30481 aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc aatacttgaa
    30541 aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag aaaatgctga
    30601 tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg catatgcgct
    30661 aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat ttgttgttgt
    30721 tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga gtccaatcac
    30781 aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg gtatcctgtc
    30841 taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg taatgtgtct
    30901 gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc cttatggcca
    30961 gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt gactcaactc
    31021 caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc tttgctactt
    31081 ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat gtttttaatt
    31141 aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg tttttaatac
    31201 tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca tttttaaata
    31261 acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca tcaggattaa
    31321 ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat ttccttatgt
    31381 tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc ttttggatat
    31441 ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt agttcaattg
    31501 atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt ttttctgcag
    31561 ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa ttgtcttata
    31621 atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta acatttctga
    31681 ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta ttgttactat
    31741 cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct ttttccgtat
    31801 cattaactat taaaagttta cttgcaatat ttattatttt catctgttct tctactattt
    31861 acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat ctatttgtta
    31921 gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta aaggatgccg
    31981 caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt attctggtag
    32041 ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta tacagatatg
    32101 taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg gttattgtct
    32161 tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg ttgccaacat
    32221 tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt tcagtattaa
    32281 accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa tttttcaaaa
    32341 gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt aatgaccgaa
    32401 aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt tgggggaggc
    32461 tgtttaagga tataatatta tttttcttgg cattttctat tcttgttatt attcttgact
    32521 ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag gagataaaaa
    32581 gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt gagttgcata
    32641 tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta gtggtaatga
    32701 acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct gcacctttta
    32761 tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca aatgaagttg
    32821 gtataccgac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca catacaaaat
    32881 atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt tggcttgagc
    32941 aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt aatttctgta
    33001 ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt aactccattg
    33061 ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata aatgttttac
    33121 tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt tgtgctctgg
    33181 agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact cttttgctga
    33241 tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag aaaattgtta
    33301 tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac gactgaccat
    33361 aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg ttaaaactct
    33421 gttatatagt attcgcgatt atgcaaagtc tgtaaatgaa catgagatac taaccgagag
    33481 taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta aaagtagtaa
    33541 atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac caaatgatta
    33601 gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt tttgagactg
    33661 taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg gagacaggta
    33721 aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag gcctcaaaac
    33781 tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct cttaaaacga
    33841 tagtggctca ggctttcatt tttcagcgtt c
//
LOCUS       DAJTRM010000037        33496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRM010000037 DAJTRM010000000
VERSION     DAJTRM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.037"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO322_001867"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO322_001867"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5906774.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO322_001868"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO322_001868"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5906775.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO322_001869"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO322_001869"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5906776.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO322_001870"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO322_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5906777.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO322_001871"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO322_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5906778.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO322_001872"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO322_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5906779.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO322_001873"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO322_001873"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5906780.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO322_001874"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO322_001874"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5906781.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO322_001875"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO322_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5906782.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO322_001876"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO322_001876"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5906783.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO322_001877"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO322_001877"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5906784.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO322_001878"
     CDS             8212..9075
                     /locus_tag="OO322_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5906785.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO322_001879"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO322_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5906786.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO322_001880"
     CDS             10411..11028
                     /locus_tag="OO322_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5906787.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO322_001881"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO322_001881"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5906788.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO322_001882"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO322_001882"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5906789.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO322_001883"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO322_001883"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5906790.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO322_001884"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO322_001884"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5906791.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO322_001885"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO322_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5906792.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO322_001886"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO322_001886"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5906793.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO322_001887"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO322_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5906794.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO322_001888"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO322_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5906795.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO322_001889"
     CDS             21859..23568
                     /locus_tag="OO322_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5906796.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO322_001890"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO322_001890"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5906797.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO322_001891"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO322_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO322_001892"
     tRNA            27625..27719
                     /locus_tag="OO322_001892"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO322_001893"
     CDS             27991..29202
                     /locus_tag="OO322_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5906798.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO322_001894"
     CDS             29614..30657
                     /locus_tag="OO322_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5906799.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="OO322_001895"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="OO322_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="OO322_001896"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="OO322_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32846..33494
                     /locus_tag="OO322_001897"
                     /pseudo
     CDS             32846..33494
                     /locus_tag="OO322_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8395 a         8804 c         8254 g         8043 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagt
//
LOCUS       DAJTRM010000038        33321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRM010000038 DAJTRM010000000
VERSION     DAJTRM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.038"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            17..1474
                     /gene="ravA"
                     /locus_tag="OO322_001898"
     CDS             17..1474
                     /gene="ravA"
                     /locus_tag="OO322_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5906800.1"
                     /translation="MSSSLEKGLYERSHAIRLCLLAALSGESVFLLGPPGIAKSLIAR
                     RLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTSGYLPEAEIVFLDE
                     IWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLI
                     RLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFEL
                     IFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQ
                     SLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTLIRLGGIFSR
                     RQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNGIG
                     FAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHAL
                     FSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1468..2919
                     /gene="viaA"
                     /locus_tag="OO322_001899"
     CDS             1468..2919
                     /gene="viaA"
                     /locus_tag="OO322_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5906801.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2924..3916)
                     /gene="asnA"
                     /locus_tag="OO322_001900"
     CDS             complement(2924..3916)
                     /gene="asnA"
                     /locus_tag="OO322_001900"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5906802.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4068..4526
                     /gene="asnC"
                     /locus_tag="OO322_001901"
     CDS             4068..4526
                     /gene="asnC"
                     /locus_tag="OO322_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5906803.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4616..5059
                     /gene="mioC"
                     /locus_tag="OO322_001902"
     CDS             4616..5059
                     /gene="mioC"
                     /locus_tag="OO322_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5906804.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5438..7327
                     /gene="mnmG"
                     /locus_tag="OO322_001903"
     CDS             5438..7327
                     /gene="mnmG"
                     /locus_tag="OO322_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5906805.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7391..8014
                     /gene="rsmG"
                     /locus_tag="OO322_001904"
     CDS             7391..8014
                     /gene="rsmG"
                     /locus_tag="OO322_001904"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5906806.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8631..9011
                     /gene="atpI"
                     /locus_tag="OO322_001905"
     CDS             8631..9011
                     /gene="atpI"
                     /locus_tag="OO322_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5906807.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9020..9835
                     /gene="atpB"
                     /locus_tag="OO322_001906"
     CDS             9020..9835
                     /gene="atpB"
                     /locus_tag="OO322_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5906808.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9882..10121
                     /gene="atpE"
                     /locus_tag="OO322_001907"
     CDS             9882..10121
                     /gene="atpE"
                     /locus_tag="OO322_001907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5906809.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10183..10653
                     /gene="atpF"
                     /locus_tag="OO322_001908"
     CDS             10183..10653
                     /gene="atpF"
                     /locus_tag="OO322_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5906810.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10668..11201
                     /gene="atpH"
                     /locus_tag="OO322_001909"
     CDS             10668..11201
                     /gene="atpH"
                     /locus_tag="OO322_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5906811.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11214..12755
                     /gene="atpA"
                     /locus_tag="OO322_001910"
     CDS             11214..12755
                     /gene="atpA"
                     /locus_tag="OO322_001910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5906812.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12806..13669
                     /gene="atpG"
                     /locus_tag="OO322_001911"
     CDS             12806..13669
                     /gene="atpG"
                     /locus_tag="OO322_001911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5906813.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13696..15078
                     /gene="atpD"
                     /locus_tag="OO322_001912"
     CDS             13696..15078
                     /gene="atpD"
                     /locus_tag="OO322_001912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5906814.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15099..15518
                     /gene="atpC"
                     /locus_tag="OO322_001913"
     CDS             15099..15518
                     /gene="atpC"
                     /locus_tag="OO322_001913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5906815.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15869..17239
                     /gene="glmU"
                     /locus_tag="OO322_001914"
     CDS             15869..17239
                     /gene="glmU"
                     /locus_tag="OO322_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5906816.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17401..19230
                     /gene="glmS"
                     /locus_tag="OO322_001915"
     CDS             17401..19230
                     /gene="glmS"
                     /locus_tag="OO322_001915"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5906817.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19392..20213
                     /locus_tag="OO322_001916"
     CDS             19392..20213
                     /locus_tag="OO322_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5906818.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20200..22308
                     /locus_tag="OO322_001917"
     CDS             20200..22308
                     /locus_tag="OO322_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5906819.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22305..23972
                     /locus_tag="OO322_001918"
     CDS             22305..23972
                     /locus_tag="OO322_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5906820.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            23975..25501
                     /locus_tag="OO322_001919"
     CDS             23975..25501
                     /locus_tag="OO322_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5906821.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25502..27118
                     /locus_tag="OO322_001920"
     CDS             25502..27118
                     /locus_tag="OO322_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5906822.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27174..27326)
                     /locus_tag="OO322_001921"
                     /pseudo
     CDS             complement(27174..27326)
                     /locus_tag="OO322_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27349..27726)
                     /locus_tag="OO322_001922"
     CDS             complement(27349..27726)
                     /locus_tag="OO322_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906823.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28136..28507)
                     /locus_tag="OO322_001923"
     CDS             complement(28136..28507)
                     /locus_tag="OO322_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906824.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28568..29065)
                     /locus_tag="OO322_001924"
     CDS             complement(28568..29065)
                     /locus_tag="OO322_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906825.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29141..29929)
                     /gene="aadA1"
                     /locus_tag="OO322_001925"
     CDS             complement(29141..29929)
                     /gene="aadA1"
                     /locus_tag="OO322_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5906826.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(29987..30511)
                     /gene="sat2"
                     /locus_tag="OO322_001926"
     CDS             complement(29987..30511)
                     /gene="sat2"
                     /locus_tag="OO322_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5906827.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30606..31079)
                     /locus_tag="OO322_001927"
     CDS             complement(30606..31079)
                     /locus_tag="OO322_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5906828.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31411..31947
                     /locus_tag="OO322_001928"
     CDS             31411..31947
                     /locus_tag="OO322_001928"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5906829.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32062..32388
                     /locus_tag="OO322_001929"
     CDS             32062..32388
                     /locus_tag="OO322_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5906830.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32576..32815)
                     /locus_tag="OO322_001930"
     CDS             complement(32576..32815)
                     /locus_tag="OO322_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5906831.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8639 a         7769 c         8427 g         8486 t
ORIGIN      
        1 ggaaagaatt tcccgcctga gcagttcgtt ggaaaagggg ctttatgaac gtagccacgc
       61 catccgcttg tgtttattag cggcattaag tggtgaaagt gtgttcctcc ttggcccgcc
      121 aggtattgcc aaaagtttga tcgcccggcg cttaaaattc gcctttcaga atgcccgcgc
      181 gtttgaatat ctgatgaccc gcttctccac gccggaagaa gtttttggtc ccctttctat
      241 tcaggcgcta aaagatgaag ggcgctatga acgtttaacc agcggttacc tgccggaagc
      301 agaaatcgtc tttctggatg agatctggaa agcgggcccg gcaattctta ataccttgct
      361 caccgccatt aacgagcgcc agttccgcaa cggtgcacac gtagaaaaaa tcccgatgcg
      421 tctactggtg gcggcctcca acgagctgcc ggaagcagac agcagtctgg aagcgttata
      481 tgaccgcatg ctgattcgtc tatggttaga taaagtgcaa gataaagcga atttccgctc
      541 catgctgacc agtcaacagg atgaaaacga caatccggtt cctgatgcct tgcaggtcac
      601 agatgaagaa tatgaacgct ggcagaaaga gattggtgaa attacgctgc ccgatcatgt
      661 atttgagctg atttttattc tgcgccagca actggataaa ttaccggatg cgccttatgt
      721 ctcggatcgt cgctggaaaa aagcgatccg cttattgcag gccagcgcct tttttagcgg
      781 tcgcagtgct gttgccccgg ttgatctcat tttgctgaaa gattgcctgt ggtatgacgc
      841 gcaaagcctg aatttgatac aacaacaaat tgatgtattg atgaccggtc atgcctggca
      901 acagcaaggg atgttgaccc gcctgggcgc gattgtgcaa cgtcacctgc aactccagca
      961 gcaacaaagc gataaaacag ccttaacgtt aattcgtctg ggcggcattt tcagccgtcg
     1021 tcagcagtat caacttcctg ttaacgttac tgcttccact ctgactctgc tgctgcaaaa
     1081 accgttaaaa ctgcatgata tggaagtggt tcatatctcc tttgagcgta gcgcgctgga
     1141 acagtggctg agtaaaggtg gtgaaattcg cggcaaactg aacggtatcg gctttgccca
     1201 gaaactgaat ctggaagttg atagcaccca acatcttgtt gtacgcgatg taagtttaca
     1261 aggcagtacg ctggcacttc ccggttcatc ggctgaaggt ctgccaggtg aaataaaaca
     1321 acaactggaa gagcttgaaa gcgactggcg taagcaacac gctttattca gcgaacagca
     1381 aaaatgtctg tttatccctg gcgactggtt aggtcgcatt gaagacagcc tacaggatgt
     1441 cggtgcacag attcgtcagg cacaacaatg ctaacgctgg atacgcttaa tgtgatgctg
     1501 gccgtcagcg aagagggatt gatcgaagag atgatcatcg cactgctggc ctcaccgcag
     1561 ctggcggtct tctttgaaaa attcccacga ctgaaggcgg caatcactga tgatgttccc
     1621 cgctggcgtg aggcgctgcg cagtcggctg aaagatgccc gagtcccgcc ggaactcacc
     1681 gaagaggtga tgtgctatca gcaaagccag ctcctctcca cgccgcagtt tattgtgcag
     1741 ctaccacaga tcctggactt actgcatcgt ctgaattccc catgggcaga acaagcccgg
     1801 cagttggttg atgctaacag cacaataact tcagcgttac acacgctttt tctccagcgt
     1861 tggcgtttaa gtctgatcgt gcaagcaacg acgttaaatc aacagctatt agaagaagaa
     1921 cgcgaacaac tgttaagtga agttcaggaa cgcatgacgc tgagcggaca acttgaaccg
     1981 attctcgcag ataacaatac cgcagctggt cgtctgtggg atatgagcgc cggtcaactt
     2041 aaacgtggcg actatcagtt tattgtgaaa tacggtgaat ttcttaacga acagccggaa
     2101 ctgaaacgcc tggcagagca gctggggcgt tctcgggaag ccaaatcaat accgcgcaac
     2161 gatgcgcaga tggaaacctt ccgcaccatg gtgcgcgaac cggcgacggt tcctgagcag
     2221 gttgatggtc tgcaacaaag cgatgatatt ttacgtctcc tgccgccaga actggcgaca
     2281 ctagggataa cggaactgga gtatgagttt taccgtcggc tggtggaaaa acagttgctc
     2341 acctatcgcc tgcacggtga gtcgtggcgt gaaaaagtgt tcgaacgtcc ggtggtacat
     2401 aaagattacg atgaacagcc gcgcgggccg tttattgtct gtgtggatac ttccggctca
     2461 atgggcggct ttaatgaaca gtgtgcgaaa gcgttctgcc tggctttgat gcgcattgct
     2521 ctcgctgaaa accggcgctg ttatattatg ctattttcca ccgagatcgt ccgttatgag
     2581 ctttcaggcc cacaaggcat cgaacaagca atccgttttt taagccagca gtttcgtggc
     2641 ggcaccgatc ttgccagttg ttttcgcgcc attatggaac gcttgcaaag cagggaatgg
     2701 tttgatgccg atgcggtggt gatttctgat tttatcgctc agcggttgcc tgacgacgtg
     2761 acgagtaaag tgaaagagct gcagcgggta catcagcatc gctttcatgc cgtggcgatg
     2821 tcggcacacg gcaaacccgg catcatgcgc attttcgatc atatctggcg ctttgatacc
     2881 gggatgcgaa gccgcctgct cagacgctgg cggcgataaa ttattacagc agagaaggga
     2941 cgctctcgcg aacagcagct ggccatactc cacactgaac ctggccgata tgcggcagtt
     3001 gcagcagcag catagtcaaa cgagactggc cgataccgcc gccgatggtc tgcggcattt
     3061 caccgcgcag cagcgcctga tgccattcca gctgcagacg atcttcatca ccggtcagcg
     3121 ccagctgatg cttcagcgtg tcggcatcca cgcggatccc catggaagaa agctcaaacg
     3181 catcttccag taccgggttc cacaccagaa tatcgccgtt caggcccgca tggcccagct
     3241 ctgacggggt gctccagtca tcataatccg gtgcgcgcac gtcgtgacga tgaccatcac
     3301 tcaacttgcc gccaatcccg acgaggaata ccgcgccaag atctttcgca atcgcccgct
     3361 cacgcccttt ggcatcgaga tccggataac gagacagtaa ctcctggctg tgtacgaagt
     3421 gaatctgatc cggcaggaac ggtgccaggc caaactcttc gttaaccgca gcttcggttg
     3481 ctttaattcc cgcccagatc gcctctaccg tgcttttcag agtcgagagt tgacgctcac
     3541 cgtcgcccat tacgcgttcc cagtcccact ggtcaacata gaccgagtgc aacggagaaa
     3601 gacggtcttc atcggggcga agggctttca tgtgcgtgta cagcccttcg cccgcgctga
     3661 agtcgtgttg ccctaaggtc tgacgtttcc acttcgccag tgaatgaacc acttcgaact
     3721 gggcatcagg cagagctttc acttttacct gcaccgcttt ttcacagccc gacaagttat
     3781 cctgcgtgcc atcccccaca cggctaagaa tcggcgcctg gacttcgatc agccccagac
     3841 gttcttccag ttggcgagaa aagtgagatt tcacgaagct aatttggcgt tgtttggcaa
     3901 tgtaagcggt tttcattttt tatactcctg cgtcctgttg cttatgatta agcaacaaaa
     3961 accgacacac atgcaataat cattcaataa aaagcctaac acacttttga ttcattaaaa
     4021 aagacggcta aaatagaatg aatcatcaat ctgcataaga aaatcctatg gaaaattatc
     4081 tgatcgacaa tctggaccgt ggcatcctgg aagcattaat gggcaatgcg cgcaccgctt
     4141 acgccgaact ggcgaaacaa tttggcgtca gtccggggac gattcacgtt cgagtagaga
     4201 aaatgaagca ggcggggatc attaccgggg cgcgtattga tgtcagcccg aagcagctcg
     4261 gttatgacgt aggctgcttt atcggcatta tattaaagag cgccaaagac tacccttccg
     4321 cgctggcaaa gctggaaagc cttgatgaag tcactgaagc ctattacaca accggccact
     4381 acagcatctt tataaaagtt atgtgccgtt cgatcgacgc tctccagcat gtacttatca
     4441 acaagatcca aacaattgat gaaattcagt ccaccgagac attgatcgtc ctgcaaaacc
     4501 cgatcatgcg taccatcaag ccctgatcgg cttttttaat cccatacttt tccacaggta
     4561 gatcccaacg cgttcacagc gtacaatacg ccactcttaa taaaggtggc ggtttatggc
     4621 agatatcact cttatcagcg gcagcaccct cggcggtgcc gaatatgtag cagaacacct
     4681 ggctgaaaag ctggaagagg tgggttttac caccgaaacg ctgcacggtc cgctgttgga
     4741 agatttatct gcctcgggga tctggctggt tatcagctcc acccacggtg ccggagatat
     4801 tccggacaac ctttctcctt tctatgaagc attgcaggaa cagaagcccg atctttctgc
     4861 ggtccgcttt ggcacaatcg gtattggcag tcgtgaatat gacacctttt gtggggctat
     4921 cgataaactc gaggcagaac tcaaaaattc cggtgcaaaa cagacaggcg aaacactgaa
     4981 gatcaacatt cttgatcacg acattccgga agatccggca gaagaatggc tgggatcgtg
     5041 gattaattta ctcaaataag tatacagatc gtgcgatcta ctgtggataa ctctgtcagg
     5101 aagcttggat caaccggtag ctatccaaag aacaaccgtt gttcagtttt tgagttgtgt
     5161 ataacccctc attctgatcc cagcttatac ggtccaggat caccgatcat tcacagttaa
     5221 tgatcctttc caggttgttg atcttaaaaa tcggatcctt gttatccaca gggcagtgcg
     5281 atcctaataa gagatcacaa tagaacagat ctctaaataa atagatcttc tttttaatac
     5341 ccaggatccc aggtctttct caagccgaca aagttgagta gaatccacgg cccgggcttc
     5401 aatccatttt cataccgcgt tatgcgaggc aatcaccatg ttttatccgg atccttttga
     5461 cgtcatcatc attggcgggg gtcatgcagg caccgaggcc gcgatggctg cggcgcgtat
     5521 gggtcaacag actctgcttt tgacacacaa tatcgacact ctggggcaga tgagctgcaa
     5581 cccggcgatc ggcggtattg ggaagggaca tctggtaaaa gaagtggatg cactcggcgg
     5641 tctgatggcg aaagcgatcg atcaagcggg tatccagttt aggatactaa acgcaagcaa
     5701 aggaccggcg gttcgcgcta cccgagctca ggcggatcgt gtgcgctacc gtcaggcggt
     5761 acgtacggcg ctggagaacc aaccgaacct gatgatcttc cagcaggcgg ttgaagatct
     5821 tattgtcgaa aacgatcgcg tggtcggtgc tgttacccaa atgggactga agttccgtgc
     5881 caaagctgtg gtgctcaccg ttgggacgtt cctcgacggc aaaattcata tcggtctgga
     5941 taattacagc ggtggccgtg ctggcgatcc gccatccatt ccgctctctc gccgtttgcg
     6001 tgaactgccg ctgcgcgttg gtcgtctgaa aaccggtact ccgccgcgta ttgatgctcg
     6061 aaccatcgac tttagcgtgc tggcgcaaca gcatggcgat aacccaatgc cggtattctc
     6121 gtttatgggc aatgcgtccc tgcatcccca gcaggtgccg tgttatatca ctcataccaa
     6181 cgagaaaacc catgatgtga tccgcagtaa cctcgatcgt agcccaatgt atgcaggggt
     6241 gatcgaaggt gtcggcccac gctactgccc gtcgatcgaa gacaaagtca tgcgcttcgc
     6301 agacagaaat cagcatcaga tcttccttga accggaagga ctgacctcta atgaaattta
     6361 tccgaacggt atctccacca gcctgccgtt cgatgtgcag atgcaaatcg tccgctctat
     6421 gcaggggatg gaaaacgcga agatcgtgcg tccgggttat gccattgagt atgacttctt
     6481 cgatcctcgc gacctgaaac cgacgctgga gagcaagttt atccaggggc tgttctttgc
     6541 tggtcagatt aacggcacta ccggttacga agaagccgct gcgcaaggtt tgctggccgg
     6601 tcttaacgct gcccgtctgt ctgctgacaa agaaggttgg gctccggcac gttctcaggc
     6661 gtatctcggc gtactggttg atgacctgtg cactttagga accaaagaac cgtatcgtat
     6721 gtttacttcg cgcgcagaat atcgtctgat gctgcgcgaa gataatgcgg atctgcgttt
     6781 gactgaaatc ggtcgtgaac tgggcctggt ggatgacgaa cgttgggcgc gctttaacga
     6841 gaaacttgag aatatcgagc gtgagcgtca gcgtctgaaa tcgacctggg taaccccgtc
     6901 ggcggaagct gcagccgaag tgaatgctca cctgactgcg ccgctttccc gtgaagccag
     6961 tggtgaagat ctgctgcgtc gtccggaaat gacttatgaa aaattaacca cgctgacgcc
     7021 gtttgcccct gcgttgacag acgaacaggc ggcggaacag gttgagattc aggttaaata
     7081 cgaaggttat atcgcgcgcc agcaagatga gatcgaaaag cagctgcgta acgagaacac
     7141 cctgctaccc gcgacactgg attaccgcca gatatccggt ctttctaacg aagtgatcgc
     7201 caaacttaac gatcacaaac cggcctctat cggccaggct tcgcgtattt ctggcgtcac
     7261 gcctgcggcc atctccattc tgctggtgtg gctgaaaaaa cagggtatgc tgcgtcgtag
     7321 cgcataacgc attaaaaatg cctggtaagc acccgcttac caggcaacgc atcaagaaca
     7381 ggtaatcacc gtgctcaaca aactctcctt actgctgaaa gacgcaggta tttcgcttac
     7441 tgatcaccag aaaaaccagc ttattgccta cgtgaatatg ctgcataaat ggaacaaagc
     7501 gtacaacctg acttcggtcc gcgatcctaa tgagatgctg gtacgccata ttctcgatag
     7561 cattgtggtg gcaccgtatc tgcaaggtga acggtttatc gatgtcggca ccggaccagg
     7621 actgccaggc attccactct ctatcgtgcg tcctgaagcc catttcactc tgttggatag
     7681 ccttggtaaa cgcgtgcgtt tccttcgtca ggtgcaacat gagcttaaac tggagaatat
     7741 tgaaccagta cagagcaggg tagaagagtt tccttcagag ccgccatttg atggcgtaat
     7801 tagccgcgct tttgcctctc tgaacgatat ggtgagctgg tgccaccatc ttcctggtga
     7861 gcaaggccgt ttctacgcgc tgaaagggca aatgccggaa gatgaaatcg ctttgttgcc
     7921 cgaagaatat caggtcgaat cagtggttaa acttcaggtt ccagccctgg atggcgaacg
     7981 tcatctggtg gtgattaaag caaataaaat ttaattttta tcaaaaaaat cataaaaaat
     8041 tgaccggtta gactgttaac aacaaccagg ttttctactg atataactgg ttacatttaa
     8101 cgccacgttc actcttttgc atcaacaaga taacgtggct ttttttggta agcagaaaat
     8161 aagtcattag tgaaaatatc agtctgctaa aaatcggcgc taagaaccat cattggctgt
     8221 taaaacatta ttaaaaatgt caatgagtgg tttttgttgt gtaaatgtta tttattaaaa
     8281 cagtatctgt ttttagactg aaatatcata aacttgtaaa ggcatcattt gccaagtaaa
     8341 taaatatgct gtgcgcgaac atgcgcaata tgtgatctga agcacgcttt atcaccagtg
     8401 tttacgcgtt atttacagtt tttcatgatc gaacaggggt agcggaaaag tcgcaattgt
     8461 atgcactgga aaaatattta aacatttatt caccttttgg ctacttattg tttgaaatca
     8521 cgggggcgca ccgtataatt tgaccgcttt ttgatgcttg actctaagcc ttaaagaaag
     8581 ttttatacga cacgcggcat acctcgaagg gagcaggagt gaaaaacgtg atgtctgtgt
     8641 cgctcgtgag tcgaaacgtt gctcggaagc ttctgctcgt tcagttactg gtggtgatag
     8701 caagtggatt gctgttcagc ctcaaagacc ccttctgggg cgtctctgca ataagcgggg
     8761 gcctggcagt ctttctgcct aacgttttgt ttatgatatt tgcctggcgt caccaggcgc
     8821 atacaccagc gaaaggccgg gtggcttgga cattcgcatt tggcgaagct ttcaaagttc
     8881 tggcgatgtt ggtgttactg gtggtggcgt tggcggtttt aaaggcggta ttcttgccgc
     8941 tgatcgttac gtgggttttg gtgctggtgg ttcagatact ggcaccggct gtaattaaca
     9001 acaaagggta aaaggcatca tggcttcaga aaatatgacg ccgcaggatt acataggaca
     9061 ccacctgaat aaccttcagc tggacctgcg tacattctcg ctggtggatc cacaaaaccc
     9121 cccagccacc ttctggacaa tcaatattga ctccatgttc ttctcggtgg tgctgggtct
     9181 gttgttcctg gttttattcc gtagcgtagc caaaaaggcg accagcggtg tgccaggtaa
     9241 gtttcagacc gcgattgagc tggtgatcgg ctttgttaat ggtagcgtga aagacatgta
     9301 ccatggcaaa agcaagctga ttgctccgct ggccctgacg atcttcgtct gggtattcct
     9361 gatgaatctg atggatttac tgcctatcga cttgctgccg tacattgctg aacatgtact
     9421 gggtctgcct gcactgcgtg tggttccgtc tgcggacgtg aacgtaacgc tgtctatggc
     9481 actgggcgta tttatcctga ttctgttcta caacatcaaa atgaaaggca tcggcggctt
     9541 cacgaaagag ttgacgctgc agccgttcaa tcactgggcg ttcattcctg tcaacttaat
     9601 ccttgaaggg gtaagcctgc tgtccaaacc agtttcactc ggtttgcgac tgttcggtaa
     9661 catgtatgcc ggtgagctga ttttcattct gattgctggt ctgttgccgt ggtggtcaca
     9721 gtggatcctg aatgtgccgt gggccatttt ccacatcctg atcattacgc tgcaagcctt
     9781 catcttcatg gttctgacga tcgtctatct gtcgatggcg tctgaagaac attaatttac
     9841 caacactact acgttttaac tgaaacaaac tggagactgt catggaaaac ctgaatatgg
     9901 atctgctgta catggctgcc gctgtgatga tgggtctggc ggcaatcggt gctgcgatcg
     9961 gtatcggcat cctcgggggt aaattcctgg aaggcgcagc gcgtcaacct gatctgattc
    10021 ctctgctgcg tactcagttc tttatcgtta tgggtctggt ggatgctatc ccgatgatcg
    10081 ctgtaggtct gggtctgtac gtgatgttcg ctgtcgcgta gtaagcgttg cttttattta
    10141 aagagcaata tcagaacgtt aactaaatag aggcattgtg ctgtgaatct taacgcaaca
    10201 atcctcggcc aggccatcgc gtttgtcctg ttcgttctgt tctgcatgaa gtacgtatgg
    10261 ccgccattaa tggcagccat cgaaaaacgt caaaaagaaa ttgctgacgg ccttgcttcc
    10321 gcagaacgag cacataagga ccttgacctt gcaaaggcca gcgcgaccga ccagctgaaa
    10381 aaagcgaaag cggaagccca ggtaatcatc gagcaggcga acaaacgccg ctcgcagatt
    10441 ctggacgaag cgaaagctga ggcagaacag gaacgtacta aaatcgtggc ccaggcgcag
    10501 gcagaaattg aagccgagcg taaacgtgcc cgtgaagagc tgcgtaagca agttgctatc
    10561 ctggctgttg ctggcgccga gaagatcatc gaacgttccg tggatgaagc tgctaacagc
    10621 gacatcgtgg ataaacttgt cgctgaactg taaggaggga ggggctgatg tctgaattta
    10681 ttacggtagc tcgcccctac gccaaagcag cttttgactt tgccgtcgaa caccaaagtg
    10741 tagaacgctg gcaggacatg ctgacgtttg ccgccgaggt aaccaaaaac gaacaaatgg
    10801 cagagcttct ctctggcgcg cttgcgccag aaacgctcgc cgagtcgttt atcgcagttt
    10861 gtggtgagca actggacgaa aacggtcaga acctgattcg ggtaatggct gaaaatggtc
    10921 gtcttaacgc gctcccggat gttctggagc agtttattca ccttcgtgcc gtgagtgagg
    10981 ctaccgctga ggtagacgtc atttccgctg ccgcactgag tgaacaacag ctcgcgaaaa
    11041 tttctgctgc gatggaaaaa cgtctgtcac gcaaagttaa gctgaattgc aaaatcgata
    11101 agtctgtaat ggctggcgtt atcatccgat cgggtgatat ggtcattgat ggcagcgtac
    11161 gcggtcgtct tgagcgcctt gcagacgtct tgcagtctta aggggactgg agcatgcaac
    11221 tgaattccac cgaaatcagc gaactgatca agcagcgcat tgctcagttc aatgttgtga
    11281 gtgaagctca caacgaaggt actattgttt ctgtaagtga cggtgttatc cgcattcacg
    11341 gcctggccga ttgtatgcag ggtgaaatga tctccctgcc gggtaaccgt tacgctatcg
    11401 cactgaacct cgagcgcgac tctgtaggtg cggttgttat gggtccgtac gctgaccttg
    11461 ccgaaggcat gaaagttaag tgtactggcc gtatcctgga agttccggtt ggccgtggcc
    11521 tgctgggccg tgtggttaac actctgggtg caccaatcga cggtaaaggt ccgctggatc
    11581 acgacggctt ctctgctgta gaagcaatcg ctccgggcgt tatcgaacgt cagtccgtag
    11641 atcagccggt acagaccggt tataaagccg ttgactccat gatcccaatc ggtcgtggtc
    11701 agcgtgaatt gatcatcggt gaccgtcaga caggtaaaac cgcactggct atcgatgcca
    11761 tcatcaacca gcgcgattcc ggtatcaaat gtatctatgt cgctatcggc cagaaagcgt
    11821 ccaccatttc taacgtggta cgtaaactgg aagagcacgg cgcactggct aacaccatcg
    11881 ttgtggtagc aaccgcgtct gaatccgctg cactgcaata cctggcaccg tatgccggtt
    11941 gcgcaatggg cgaatacttc cgtgaccgcg gtgaagatgc gctgatcatt tacgatgacc
    12001 tgtctaaaca ggctgttgct taccgtcaga tctccctgct gctccgtcgt ccgccaggac
    12061 gtgaagcatt cccgggcgac gttttctacc tccactctcg tctgctggag cgtgctgcac
    12121 gtgttaacgc cgaatacgtt gaagccttca ccaaaggtga agtgaaaggg aaaaccggtt
    12181 ctctgaccgc actgccgatt atcgaaactc aggcgggtga cgtttctgcg ttcgttccga
    12241 ccaacgtaat ctccattacc gatggtcaga tcttcctgga aaccaacctg ttcaacgccg
    12301 gtattcgtcc tgcggttaac ccgggtattt ccgtatcccg tgttggtggt gcagcacaga
    12361 ccaagatcat gaaaaaactg tccggtggta tccgtaccgc tctggcacag tatcgtgaac
    12421 tggcagcgtt ctctcagttt gcatccgacc ttgacgatgc aacacgtaag cagcttgacc
    12481 acggtcagaa agtgaccgaa ctgctgaaac agaaacagta tgcgccgatg tccgttgcgc
    12541 agcagtctct ggttctgttc gcagcagaac gtggttacct ggcggatgtt gaactgtcga
    12601 aaattggcag cttcgaagcc gctctgctgg cttacgtcga ccgtgatcac gctccgttga
    12661 tgcaagagat caaccagacc ggtggctaca acgacgaaat cgaaggcaag ctgaaaggca
    12721 tcctcgattc cttcaaagca acccaatcct ggtaacgtct ggaggcttgc cttagggcag
    12781 gccgcaaggc attgaggaga agctcatggc cggcgcaaaa gagatacgta gtaagatcgc
    12841 aagcgtccag aacacgcaaa agatcactaa agcgatggag atggtcgccg cttccaaaat
    12901 gcgtaaatcg caggatcgca tggcggccag ccgtccttat gcagaaacca tgcgcaaagt
    12961 gattggtcac cttgcacacg gtaatctgga atataagcac ccttacctgg aagaccgcga
    13021 cgttaaacgc gtgggctacc tggtggtgtc gaccgaccgt ggtttgtgcg gtggtttgaa
    13081 cattaacctg ttcaaaaaac tgctggcgga aatgaagacc tggaccgaca aaggcgttca
    13141 atgcgacctc gcaatgatcg gctcgaaagg cgtgtcgttc ttcaactccg tgggcggcaa
    13201 tgttgttgcc caggtcaccg gcatggggga taacccttcc ctgtccgaac tgatcggtcc
    13261 ggtaaaagtg atgttgcagg cctacgacga aggccgtctg gacaagcttt acattgtcag
    13321 caacaaattt attaacacca tgtctcaggt tccgaccatc agccagctgc tgccgttacc
    13381 ggcatcagat gatgatgatc tgaaacataa atcctgggat tacctgtacg aacccgatcc
    13441 gaaggcgttg ctggataccc tgctgcgtcg ttatgtcgaa tctcaggttt atcagggcgt
    13501 ggttgaaaac ctggccagcg agcaggccgc ccgtatggtg gcgatgaaag ccgcgaccga
    13561 caatggcggc agcctgatta aagagctgca gttggtatac aacaaagctc gtcaggccag
    13621 cattactcag gaactcaccg agatcgtctc gggggccgcc gcggtttaaa caggttattt
    13681 cgtagaggat ttaagatggc tactggaaag attgtccagg taatcggcgc cgtagttgac
    13741 gtcgaattcc ctcaggatgc cgtaccgcgc gtgtacgatg ctcttgaggt gcaaaatggt
    13801 aatgagcgtc tggtgctgga agttcagcag cagctcggcg gcggtatcgt gcgtaccatc
    13861 gcaatgggtt cctccgacgg tctgcgtcgc ggtctggatg taaaagacct cgaacacccg
    13921 atcgaagtcc cggtaggtaa agcgactctg ggccgtatca tgaacgtact gggtgaaccg
    13981 gtcgacatga aaggcgagat cggtgaagaa gagcgttggg cgattcaccg cgcagcacct
    14041 tcctacgaag agctgtcaaa ctctcaggaa ctgctggaaa ccggtatcaa agttatcgac
    14101 ctgatgtgtc cgttcgctaa gggcggtaaa gttggtctgt tcggtggtgc gggtgtaggt
    14161 aaaaccgtaa acatgatgga gctcattcgt aacatcgcga tcgagcactc cggttactct
    14221 gtgtttgcgg gcgtaggtga acgtactcgt gagggtaacg acttctacca cgaaatgacc
    14281 gactccaacg ttatcgacaa agtatccctg gtgtatggcc agatgaacga gccgccggga
    14341 aaccgtctgc gcgttgctct gaccggtctg accatggctg agaaattccg tgacgaaggt
    14401 cgtgacgttc tgctgttcgt tgacaacatc tatcgttaca ccctggccgg tacggaagta
    14461 tccgcactgc tgggccgtat gccttcagcg gtaggttatc agccgaccct ggcggaagag
    14521 atgggcgttc tgcaggaacg tatcacctcc accaaaactg gttctatcac ctccgtacag
    14581 gcggtatacg tacctgcgga tgacttgact gacccgtctc cggcaaccac ctttgcgcac
    14641 cttgacgcaa ccgtggtact gagccgtcag atcgcgtctc tgggtatcta cccggccgtt
    14701 gacccgctgg actccaccag ccgtcagctg gacccgctgg tggttggtca ggaacactac
    14761 gacaccgcgc gtggcgttca gtccatcctg caacgttatc aggaactgaa agacatcatc
    14821 gccatcctgg gtatggatga actgtctgaa gaagacaaac tggtggtagc gcgtgctcgt
    14881 aagatccagc gcttcctgtc ccagccgttc ttcgtggcag aagtattcac cggttctccg
    14941 ggtaaatacg tctccctgaa agacaccatc cgtggcttta aaggcatcat ggaaggcgaa
    15001 tacgatcacc tgccggagca ggcgttctac atggtcggtt ccatcgaaga agctgtggaa
    15061 aaagccaaaa aactttaacg ccttaatcgg agggtgatat ggcaatgact taccacctgg
    15121 acgtcgtcag cgcagagcaa caaatgttct ctggtctggt cgagaaaatc caggtaacgg
    15181 gtagcgaagg tgaactgggg atctaccctg gccacgcacc gctgctcacc gccattaagc
    15241 ctggtatgat tcgcatcgtg aaacagcacg gtcacgaaga gtttatctat ctgtctggcg
    15301 gcattcttga agtgcagcct ggcaacgtga ccgttctggc cgacaccgca attcgcggcc
    15361 aggatctcga cgaagcgcga gccatggaag cgaaacgtaa ggctgaagag cacattagca
    15421 gctctcacgg cgacgtagat tacgctcagg cgtctgcgga actggccaaa gcgatcgcgc
    15481 agctgcgcgt tatcgagttg accaaaaaag cgatgtaaca ccggcttgaa aagcacaaaa
    15541 gccagtctgg aaacaggctg gcttttttta cgcgttgacc cgtcctgaag agcgttcaca
    15601 tagatcctgc tgatataaaa cacccctgtt ttcccgttta ttcattgatc gaaataagag
    15661 caaaaacatc cacctgacgc ttaaatgaag gtactgcctt aattttctgc agataaaagg
    15721 cgtgacgatg gtcgaaaatg gcactttcgt cagcggggat aatccgttat tgaacaattt
    15781 atcctctgtc catttcacga tgaaaaaaat gtagtttttt caaggtgaag cggtttaaat
    15841 tcgttctcaa attacagtca ggacgcgtat gttgaataat gctatgagcg tagtgatcct
    15901 tgccgcaggc aaaggcacgc gcatgtattc cgatcttccg aaagtgctgc atacccttgc
    15961 cgggaaagcg atggttcagc atgtcattga tgctgcgaat gaattaggcg cagcgcacgt
    16021 tcacctggtg tacggtcacg gcggcgatct gcttaaacag gcgctgaaag acgacaacct
    16081 taactgggtg cttcaggcag agcagctggg tacgggtcat gcaatgcagc aggccgcacc
    16141 tttctttgcc gatgatgaag acattttaat gctctacggc gacgtgccgc tgatctctgt
    16201 cgaaacactc cagcgcctgc gtgatgctaa accccagggt ggcattggtc tgctgacggt
    16261 gaaactggat gatccgaccg gttatggacg tatcacccgt gaaaatggca aagttaccgg
    16321 cattgttgag cacaaagatg ccaccgacga gcagcgtcag attcaggaga tcaacaccgg
    16381 cattctgatt gccaacggcg cagatatgaa acgctggctg gcgaagctga ccaacaataa
    16441 tgctcagggc gaatactaca tcaccgacat tattgcgctg gcgtatcagg aagggcgtga
    16501 aatcgtcgcc gttcatccgc aacgtttaag cgaagtagaa ggtgtgaata accgcctgca
    16561 actctcccgt ctggagcgcg tttaccagtc cgaacaggct gaaaaactgc tgttagcagg
    16621 cgttatgctg cgcgatccgg cgcgttttga tctgcgcggt acgcttactc acgggcgcga
    16681 tgttgaaatt gatactaacg ttatcatcga gggcaacgtg actctcggtc atcgcgtgaa
    16741 aatcggcacc ggttgcgtga ttaaaaacag cgtgattggc gatgattgcg aaatcagtcc
    16801 gtataccgtt gtggaagacg cgaatctggc agcggcctgt actattggcc cgtttgcccg
    16861 tctgcgtcct ggtgctgagt tgctggaagg tgcacacgtt ggtaacttcg ttgagatgaa
    16921 aaaagcgcgt ctgggtaaag gcacgaaagc cgggcatctg acttacctgg gcgatgcgga
    16981 aattggcgat aacgttaaca tcggcgcggg aaccattacc tgcaactacg atggtgcgaa
    17041 taaatttaag accattatcg gcgacgatgt gtttgtcggt tccgacactc agctggtggc
    17101 cccggtaaca gtaggcaaag gcgcgaccat tgctgcgggt acaactgtga cgcgtaatgt
    17161 cggcgaaaat gcattagcta tcagccgtgt gccgcagact cagaaagaag gctggcgtcg
    17221 tccggtaaag aaaaagtaat tctggccggg taatccggtc atctgggatg aggagataac
    17281 ataatctccc tcccacaagc agtaactata aaaataaccc cactctctac aaggctcggg
    17341 gcgcccgaaa aaacgggcat acaggttgac cgacaacgat ataaatcgga atcaaaaact
    17401 atgtgtggaa ttgttggcgc gatcgcgcaa cgtgatgtag cagaaatcct tcttgaaggt
    17461 ttacgtcgtc tggaataccg cggatatgac tctgccggtc tggccgttgt tgatgcggaa
    17521 ggtcatatga ctcgcctgcg tcgcctcggt aaagtccaga tgctggctca ggcagcggaa
    17581 gaacatcctc tgcatggcgg caccggtatt gctcacactc gctgggcgac ccacggtgaa
    17641 ccttcagaag ctaacgcgca tccgcatgtt tctgaacaca ttgtggtggt gcataatggc
    17701 atcatcgaaa accatgaacc gctgcgtgaa gagttaaaag cgcgtggcta taccttcgtt
    17761 tctgaaaccg acaccgaagt gattgcccat ctggtgaact gggagctgaa acaaggcggg
    17821 actctgcgtg aggctgttct gcgtgctatc ccgcagctgc gcggtgcgta cggtacagtg
    17881 atcatggact cccgtcaccc ggataccctg ctagcggcac gttctggtag tccgctggtg
    17941 attggcctgg gtatgggcga aaactttatc gcttctgacc agctggcgct gttgccggtg
    18001 acccgtcgct ttatcttcct tgaagagggc gatattgcgg aaatcactcg ccgttcggta
    18061 aacatcttcg ataaaactgg cgcggaagta aaacgtcagg atatcgaatc caatctgcaa
    18121 tatgacgcgg gcgataaagg tatttaccgt cactacatgc agaaagagat ctacgaacag
    18181 ccgaacgcga taaaaaacac ccttaccggg cgcatcagcc acggtcaggt tgatttaagc
    18241 gagctgggac cgaacgccga cgaactgctg tcgaaggttg agcatattca gatcctcgcc
    18301 tgtggtactt cttataactc cggtatggtt tcccgctact ggtttgaatc gctggcaggt
    18361 attccgtgcg acgtcgaaat cgcctctgaa ttccgctatc gtaaatctgc cgtgcgtcgt
    18421 aacagcctga tgatcacctt gtcacagtct ggcgaaaccg cggataccct ggcgggcctg
    18481 cgtctgtcga aagagctggg ttaccttggt tcgctggcaa tctgtaacgt tccgggttct
    18541 tctctggtgc gcgaatccga tctggcgctg atgaccaacg cgggtacgga aatcggcgtg
    18601 gcgtccacta aagcattcac cactcagtta actgtgctgt tgatgctggt ggcgaagctg
    18661 tctcgcctga aaggtctgga tgcctccatt gaacatgaca ttgtgcatgg tctgcaggcg
    18721 ctgccgagcc gtattgagca gatgctgtct caggataaac gcattgaagc tctggcagaa
    18781 gatttctctg acaaacatca cgcgctgttc ctgggccgtg gcgatcagta cccaatcgcg
    18841 ctggaaggcg cattgaagct gaaagagatc tcttacattc acgctgaagc ctacgctgca
    18901 ggcgagctga aacacggccc gctggcgctg attgacgcgg atatgccggt tatcgtcgtt
    18961 gcaccgaaca acgaactgtt ggaaaaactg aaatccaaca ttgaagaagt tcgcgcgcgt
    19021 ggcggtcaat tgtatgtctt cgccgatcag gatgcgggtt ttgtaagtag cgataacatg
    19081 cacatcatcg agatgccgca tgtggaagag gtgattgcac cgatcttcta caccgttccg
    19141 ctgcagctgc tggcttacca tgtcgcgctg atcaaaggca ccgacgttga ccagccgcgt
    19201 aacctggcaa aatcggttac ggttgagtaa tcactatcat agataaaaaa gggctgctgt
    19261 gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt
    19321 aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact
    19381 ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta
    19441 ttaaagaggg gcgtggccaa gggcatggta aagactatat tccatggcta acagtacaag
    19501 aagttccttc ttcaggtcgt tcccaccgta tttattctca taagacggga cgagtccatc
    19561 atttgctatc tgacttagag cttgctgttt ttctcagtct tgagtgggag agcagcgtgc
    19621 tagatatacg cgagcagttc cccttattac ctagtgatac caggcagatt gcaatagata
    19681 gtggtattaa gcatcctgtt attcgtggtg tagatcaggt tatgtctact gattttttag
    19741 tggactgcaa agatggtcct tttgagcagt ttgctattca agtcaaacct gcagcagcct
    19801 tacaagacga gcgtacctta gaaaaactag aactagagcg tcgctattgg cagcaaaagc
    19861 aaattccttg gttcattttt actgataaag aaataaatcc cgtagtaaaa gaaaatattg
    19921 aatggcttta ttcagtgaaa acagaagaag tttctgcgga gcttttagca caactatccc
    19981 cattggccca tatcctgcaa gaaaaaggag atgaaaacat tatcaatgtc tgtaagcagg
    20041 ttgatattgc ttatgatttg gagttaggca aaacattgag tgagatacga gccttaaccg
    20101 caaatggttt tattaagttc aatatttata agtctttcag ggcaaataag tgtgcagatc
    20161 tctgtattag ccaagtagtg aatatggagg agttgcgcta tgtggcaaat taatgaggtt
    20221 gtgctatttg ataatgatcc gtatcgcatt ttggctatag aggatggcca agttgtctgg
    20281 atgcaaataa gcgctgataa aggagttcca caagctaggg ctgagttgtt gctaatgcag
    20341 tatttagatg aaggccgctt agttagaact gatgaccctt atgtacatct tgatttagaa
    20401 gagccgtctg tagattctgt cagcttccag aagcgcgagg aggattatcg aaaaattctt
    20461 cctattatta atagtaagga tcgtttcgac cctaaagtca gaagcgaact cgttgagcat
    20521 gtggtccaag aacataaggt tactaaggct acagtttata agttgttacg ccgttactgg
    20581 cagcgtggtc aaacgcctaa tgcattaatt cctgactaca aaaacagcgg tgcaccaggg
    20641 gaaagacgtt cagcgacagg aacagcaaag attggccgag ccagagaata tggtaagggt
    20701 gaaggaacca aggtaacgcc cgagattgaa cgccttttta ggttgaccat agaaaagcac
    20761 ctgttaaatc aaaaaggtac aaagaccacc gttgcctata gacgatttgt ggacttgttt
    20821 gctcagtatt ttcctcgcat tccccaagag gattacccaa cactacgtca gtttcgttat
    20881 ttttatgatc gagaataccc taaagctcag cgcttaaagt ctagagttaa agcaggggta
    20941 tataaaaaag acgtacgacc cttaagtagt acagccactt ctcaggcgtt aggccctggg
    21001 agtcgttatg agattgatgc cacgattgct gatatttatt tagtggatca tcatgatcgc
    21061 caaaaaatca taggaagacc aacgctttac attgtgattg atgtgtttag tcggatgatc
    21121 acgggctttt atatcggctt tgaaaatccg tcttatgtgg tggcgatgca ggcttttgta
    21181 aatgcttgct ctgacaaaac ggccatttgt gcccagcatg atattgagat tagtagctca
    21241 gactggccgt gtgtaggttt gccagatgtg ttgctagcgg accgtggcga attaatgagt
    21301 catcaggtcg aagccttagt ttctagtttt aatgtgcgag tggaaagtgc tccacctaga
    21361 cgtggcgatg ctaaaggcat agtggaaagc acttttagaa cactacaagc cgagtttaag
    21421 tcctttgcac ctggcattgt agagggcagt cggatcaaaa gccatggtga aacagactat
    21481 aggttagatg catctctgtc ggtatttgag ttcacacaaa ttattttgcg tacgatctta
    21541 ttcagaaata accatctggt gatggataaa tacgatcgag atgctgattt tcctacagat
    21601 ttaccgtcta ttcctgtcca gctatggcaa tggggtatgc agcatcgtac aggtagttta
    21661 agggctgtgg agcaagagca gttgcgagta gcgttactgc ctcgccgaaa ggtctctatt
    21721 tcttcatttg gcgttaattt gtggggtttg tattactcgg ggtcagagat tctgcgtgag
    21781 ggttggttgc agcggagcac tgatatagct agacctcaac atttagaagc ggcttatgac
    21841 ccagtgctgg ttgatacgat ttatttgttt ccgcaagttg gcagccgtgt attttggcgc
    21901 tgtaatctga cggaacgtag tcggcagttt aaaggtctct cattttggga ggtttgggat
    21961 atacaagcac aagaaaaaca caataaagcc aatgcgaagc aggatgagtt aactaaacgc
    22021 agggagcttg aggcgtttat tcagcaaacc attcagaaag cgaataagtt aacgcccagt
    22081 actactgagc ccaaatcaac acgcattaag cagattaaaa ctaataaaaa agaagccgtg
    22141 acctcggagc gtaaaaaacg tgcggagcat ttgaagccaa gctcttcagg tgatgaggct
    22201 aaagttattc ctttcaacgc agtggaagcg gatgatcaag aagattacag cctacccaca
    22261 tacgtgcctg aattatttca ggatccacca gaaaaggatg agtcatgagt gctacccgga
    22321 ttcaagcagt ttatcgtgat acgggggtag aggcttatcg tgataatcct tttatcgagg
    22381 ccttaccacc attacaagag tcagtgaata gtgctgcatc actgaaatcc tctttacagc
    22441 ttacttcctc tgacttgcaa aagtcccgtg ttatcagagc tcataccatt tgtcgtattc
    22501 cagatgacta ttttcagcca ttaggtacgc atttgctact aagtgagcgt atttcggtca
    22561 tgattcgagg tggctacgta ggcagaaatc ctaaaacagg agatttacaa aagcatttac
    22621 aaaatggtta tgagcgtgtt caaacgggag agttggagac atttcgcttt gaggaggcac
    22681 gatctacggc acaaagctta ttgttaattg gttgttctgg tagtgggaag acgacctctc
    22741 ttcatcgtat tctagccacg tatcctcagg tgatttacca tcgtgaactc aatgtagagc
    22801 aggtggtgta tttgaaaata gactgctcgc ataatggttc gctaaaagaa atctgcttga
    22861 attttttcag agcgttggat cgagccttgg gctcgaacta tgagcgtcgt tatggcttaa
    22921 aacgtcatgg tatagaaacc atgttggctt tgatgtcgca aatagccaat gcacatgctt
    22981 tagggttgtt ggttattgat gaaattcagc atttaagccg ctctcgttcg ggtggatctc
    23041 aagagatgct gaactttttt gtgacgatgg tgaatattat tggcgtacca gtgatgttga
    23101 ttggtacccc taaagcacga gagatttttg aggctgattt gcggtctgca cgtagagggg
    23161 cagggtttgg agctatattc tgggatccta tacaacaaac gcaacgtgga aagcccaatc
    23221 aagagtggat cgcttttacg gataatcttt ggcaattaca gcttttacaa cgcaaagatg
    23281 cgctgttatc ggatgaggtc cgtgatgtgt ggtatgagct aagccaagga gtgatggaca
    23341 ttgtagtaaa actttttgta ctcgctcagc tccgtgcgct agctttaggc aatgagcgta
    23401 ttaccgctgg tttattgcgg caagtgtatc aagatgagtt aaagcctgtg caccccatgc
    23461 tagaggcatt acgctcgggt atcccagaac gcattgctcg ttattctgat ctagtcgttc
    23521 ccgagattga taaacggtta atccaacttc agctagatat cgcagcgata caagaacaaa
    23581 caccagaaga aaaagccctt caagagttag ataccgaaga tcagcgtcat ttatatctga
    23641 tgctgaaaga ggattacgat tcaagcctgt taattcccac tattaaaaaa gcgtttagcc
    23701 agaatccaac gatgacaaga caaaagttac tgcctcttgt tttgcagtgg ttgatggaag
    23761 gcgaaacggt agtgtcagaa ctagaaaagc cctccaagag taaaaaggtt tcggctataa
    23821 aggtagtcaa gcccagcgac tgggatagct tgcctgatac ggatttacgt tatatctatt
    23881 cacaacgcca acctgaaaaa accatgcatg aacggttaaa agggaaaggg gtaatagtgg
    23941 atatggcgag cttatttaaa caagcaggtt agccatgaga aactttcctg ttccgtactc
    24001 gaatgagctg atttatagca ctattgcacg ggcaggcgtt tatcaaggga ttgttagtcc
    24061 taagcagctg ttggatgagg tgtatggcaa ccgcaaggtg gtcgctacct taggtctgcc
    24121 ctcgcattta ggtgtgatag caagacatct acatcaaaca ggacgttacg ctgttcagca
    24181 gcttatttat gagcatacct tattcccttt atatgctccg tttgtaggca aggagcgccg
    24241 agacgaagct attcggttaa tggagtacca agcgcaaggt gcggtgcatt taatgctagg
    24301 agtcgctgct tctagagtta agagcgataa ccgctttaga tactgccctg attgcgttgc
    24361 tcttcagcta aataggtatg gggaagcctt ttggcaacga gattggtatt tgcccgcttt
    24421 gccatattgt ccaaaacacg gtgctttagt cttctttgat agagctgtag atgatcaccg
    24481 acatcaattt tgggctttgg gtcatactga gctgctttca gactacccca aagactccct
    24541 atctcaatta acagcactag ctgcttatat agcccctctg ttagatgctc cacgagcgca
    24601 agagctttcc ccaagccttg agcagtggac gctgttttat cagcgcttag cgcaggatct
    24661 agggctaacc aaaagcaagc acattcgtca tgacttggtg gcggagagag tgaggcagac
    24721 ttttagtgat gaggcactag agaaactgga tttaaagttg gcagagaaca aggacacgtg
    24781 ttggctgaaa agtatattcc gtaagcatag aaaagccttt agttatttac agcatagtat
    24841 tgtgtggcaa gccttattgc caaaactaac ggttatagaa gcgctacagc aggcaagtgc
    24901 tcttactgag cactctataa cgacaagacc tgttagccag tctgtgcaac ctaactctga
    24961 agatttatct gttaagcata aagactggca gcaactagtg cataaatacc aaggaattaa
    25021 ggcggcaaga cagtctttag agggtggggt gctatacgct tggctttacc gacatgacag
    25081 ggattggcta gttcactgga atcaacagca tcaacaagag cgtctggcac ccgcccctag
    25141 agttgattgg aaccaaagag atcgaattgc tgtacgacaa ctattaagaa tcataaagcg
    25201 tctagatagt agccttgatc acccaagagc gacatcgagc tggctgttaa agcaaactcc
    25261 taacggaacc tctcttgcaa aaaatctaca gaaactgcct ttggtagcgc tttgcttaaa
    25321 gcgttactca gagagtgtgg aagattatca aattagacgg attagccaag cttttattaa
    25381 gcttaaacag gaagatgttg agcttaggcg ctggcgatta ttaagaagtg caacgttatc
    25441 taaagagcgg ataactgagg aagcacaaag attcttggaa atggtttatg gggaagagtg
    25501 agtggttagg ctagctacat ttaatgacaa tgtgcaggtt gtacatattg gtcatttatt
    25561 ccgtaactcg ggtcataagg agtggcgtat ttttgtttgg tttaatccaa tgcaagaacg
    25621 gaaatggact cgatttactc atttgccttt attaagtcga gctaaggtgg ttaacagtac
    25681 aacaaagcaa ataaataagg cggatcgtgt gattgagttt gaagcatcgg atcttcaacg
    25741 agccaaaata atcgattttc ctaatctctc gtcctttgct tccgtacgca acaaggatgg
    25801 agcgcagagt tcatttattt acgaagctga aacaccatat agcaagactc gttatcacat
    25861 cccacagtta gagctagctc ggtcattatt tttaattaac tcctatttct gtcgaagctg
    25921 tttgagcagt accgctttac agcaagagtt cgacgttcag tatgaggttg agcgagatca
    25981 tttagagata aggatcttac ccagttcatc gtttcctaaa ggggcgttag agctgtcggc
    26041 cgtagtgcag cttttggttt ggttgttttc ggatcaagat gttatggatt cgtatgaaag
    26101 tatttttagg cactatcaac aaaatagaga aattaagaac ggcgttgaaa gctggtgctt
    26161 tagctttgac cctccgccca tgcagggttg gaaattacat gtaaaaggac gttcttctaa
    26221 cgaggataag gattatttag ttgaggaaat agtaggttta gaaatcaacg ctatgcttcc
    26281 tagcacaaca gctattagcc atgcctcttt tcaggaaaag gaggcaggtg atggtagtac
    26341 gcagcacata gcggtttcaa cagagtcagt tgttgatgat gagcatctac agttggacga
    26401 tgaggaaaca gccaatatag acacagacac acgagtcata gaggctgagc cgacatggat
    26461 aagttttagt agacctagtc gaattgaaaa atctcgcagg gcaagaaaaa gtagccaaac
    26521 tattttagaa aaagaagaag caacaacaag tgaaaatagt aatttggtta gtactgatga
    26581 gccacactta ggtggtgtcc tagcagcggc agatgtgggt gggaagcagg atgcaaccaa
    26641 ttacaactct atttttgcta atcgatttgc tgcttttgat gagctacttt caattctaaa
    26701 aactaaattt gcatgtcggg tgctttttga agaaaccttg gttttgccaa aagttgggcg
    26761 tagccgatta catctgtgta aagatggctc accaagagtg attaaagccg ttggggtgca
    26821 acgtaatggc agtgaatttg tattgctaga ggtggatgca tcggatgggg tgaaaatgct
    26881 ttctaccaaa gtgttgagtg gcgttgatag cgaaacatgg cggaatgatt ttgaaaagat
    26941 acggcgtgga gtggtgaaga gctcattgaa ttggccaaat agtttgtttg atcaattata
    27001 tggacaagac gggcatagag gggtgaatca tccaaagggg ttgggggagc tgcaagtatc
    27061 gagagaggat atggaagggt gggctgagag agtggttaga gagcaattta cgcattaaag
    27121 gaatgactga aagagcctgt aaaccctttt gtgtaagtgc ttttgccggt cagttaaagg
    27181 tggccattta aacggtcacc aaattcgatc ataaaacggt tcatggccgg cttccagttg
    27241 cggatcggca tcgtccattt cttggtcgcc gcctggatag ccaggtacac caccttcatc
    27301 gctgattcgt ccgtagggaa caccttgagt tcatattaat ggaattttct acaaatagcc
    27361 tccgtggttt tgagggggga ttacagacga tccatagtag taatccaatg agttcttgag
    27421 cgcggcgaca tgtttggacg ccttggcaaa aattagagcc tgcttgaagt gcaggcgagc
    27481 acgtgcttgg acgattgatc cattcgcggt caaaaactca atcttggatg acagcgtgtc
    27541 aggaaatcca atatcgaagt cccgccccgt gtcaaagtag ggcatttcat gatcaaagga
    27601 cgaagcttcc caggccactt ccctaatatt ccagccgcta gaaacaagat ttcgtacgac
    27661 ttcatcaact tttttgcttt ccaacttgtc acaaagtgca taggcaagag ctttggcggc
    27721 agacacgtgc gtttcctctt aaacataggt cgaacggata cccgaggcaa agcggagcat
    27781 tagtcccaaa gcatctccgg tagatccgga ggctccatac tgaaggcatg gcatcttggg
    27841 cattgatatt tgaaacttct ctctagatag cagggatttc ttggccttgc ctcatgctga
    27901 tagcttgtaa atatcacacg atccttagcc ccacaaagtg agcatactcc gtagctttca
    27961 tttatagaaa tcgtcgttag attgtcgcag catgtgcacg ttccgactat ttgctcagga
    28021 agcatcgcca taccttggcc taggtaaaga agatcagact cgtagccaca tcggtggcaa
    28081 ctgagcttgt acgcaatgcc catctggttg tctcacctga atcgtaacgt cagagctatt
    28141 catccatcca ttcggcatca atgtatgcca ctaagccgac cagctgaccg tttggactga
    28201 agtatgttgc ctgcacccca cttgacatct catcaatacc accgcactgc gctaggcgcc
    28261 ggtcccactt gcccacagaa gcgtcaggat cggctgagtt ccccaagacc cttgtgagcc
    28321 atgcctcctt taactcaacc tctcgaatga ttgcgtcggc cagctcctcc gcgttatcta
    28381 gttcgacata gtctggaatt cggacgtacg ttctgaacac agctggatac ttacttgggc
    28441 gattgtcata catgacatca acaatgtacc cgtttgtgta accgtctctt ggaggttcgt
    28501 atgacactag tggttcccct cagcttgcga ctagatgttg aggcctaaca ttttattaga
    28561 gagcaggcta gttgcttaga tacatgatct tcaggccgtt atctgtcagg gcaagcgaaa
    28621 attggccatt tatgacgacc aatgccccgc agaagctccc atctttgccg ccatagacgc
    28681 cgcgcccccc ttttggggtg tagaacatcc ttttgccaga tgtggaaaag aagttcgttg
    28741 tcccattgtt ggcaatgacg tagtagccgg cgaaagtgcg agacccattt gcgctatata
    28801 taagcctacg atttccgttg cgactattgt cgtaattgga tgaactatta tcgtagttgc
    28861 tctcagagtt gtcgtaattt gatggactat tgtcgtaatt gcttatggag ttgtcgtagt
    28921 tgcttggaga aatgtcgtag ttggatgggg agtagtcata gggaagacga gcttcatcca
    28981 ctaaaacaat tggcaggtca gcaagtgcct gccccgatgc catcgcaagt acgaggctta
    29041 gaaccacctt caacagatcg cgcatagtct tccccagctc tctaacgctt gagttaagcc
    29101 gcgccgcgaa gcggcgtcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg
    29161 tgatctcgcc tttcacgtag tgaacaaatt cttccaactg atctgcgcgc gaggccaagc
    29221 gatcttcttg tccaagataa gcctgcctag cttcaagtat gacgggctga tactgggccg
    29281 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg
    29341 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg
    29401 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa
    29461 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt
    29521 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa
    29581 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa
    29641 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag
    29701 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc
    29761 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg
    29821 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa
    29881 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact
    29941 cctgaattaa gccgcgccgc gaagcggtgt cggcttgaat gaattgttag gcgtcatcct
    30001 gtgctcccga gaaccagtac cagtacatcg ctgtttcgtt cgagacttga ggtctagttt
    30061 tatacgtgaa caggtcaatg ccgccgagag taaagccaca ttttgcgtac aaattgcagg
    30121 caggtacatt gttcgtttgt gtctctaatc gtatgccaag gagctgtctg cttagtgccc
    30181 actttttcgc aaattcgatg agactgtgcg cgactccttt gcctcggtgc gtgtgcgaca
    30241 caacaatgtg ttcgatagag gctagatcgt tccatgttga gttgagttca atcttcccga
    30301 caagctcttg gtcgatgaat gcgccatagc aagcagagtc ttcatcagag tcatcatccg
    30361 agatgtaatc cttccggtag gggctcacac ttctggtaga tagttcaaag ccttggtcgg
    30421 ataggtgcac atcgaacact tcacgaacaa tgaaatggtt ctcagcatcc aatgtttccg
    30481 ccacctgctc agggatcacc gaaatcttca tatgacgcct aacgcctggc acagcggatc
    30541 gcaaacctgg cgcggctttt ggtacaaaag gcgtgacagg tttgcgaatc cgttgctgcc
    30601 acttgttaac ccttttgcca gatttggtaa ctataattta tgttagaggc gaagtcttgg
    30661 gtaaaaactg gcctaaaatt gctggggatt tcaggaaagt aaacatcacc ttccggctcg
    30721 atgtctattg tagatatatg tagtgtatct acttgatcga tcaggctttt gtatatctcc
    30781 ccaccacctg aaacaatgac atgatccgtt attttcttta ggttggttaa agcatcttta
    30841 attgatggaa agatcaatac gttctcattg tcagatgtaa aacttgaacg tgttacgacc
    30901 gcatactttc ggttgggtaa tgctcccatt gattcaaaag tcttgcgtcc aaccaacagc
    30961 cattggttat aggtaatagc tttaaacagg agctgttcac ctttggcact ccatggaata
    31021 tcagggccat tcccgataac tccattcttc gatatagcta ccattagtga tagtttcaca
    31081 attcttcctc agaggttaac attttattag taagcatgct cgttttgtca cccgctgatg
    31141 cttaccgtta attaatataa tcacttgtat taaatagact ttctttaaaa atacaagaca
    31201 ctctgttatt acaaatcgtg catgccgtct atcctgcttg cacgatgcac attatctcac
    31261 ccaaagaact cataacattt taatttattt agcattaaat taaaactcta ccggtaaaca
    31321 agcatctcta ggcgtaaaaa acaggcactt ttttaaatcc cagctagaat aggctgtata
    31381 ggcagacagt tgcaagacaa ggagcgtttt atgtctaaca gtccattttt aaattctata
    31441 cgcacggata tgcgacaaaa aggttatgcg ctgaaaactg aaaaaactta cctgcactgg
    31501 attaagcgtt ttattctgtt tcacaaaaaa cgtcatcctc agaccatggg cagtgaagag
    31561 gtcaggctgt ttttatccag cttagcaaac agcagacatg tagccataaa cacgcagaaa
    31621 atcgctttaa atgccctagc ttttttgtac aacaggtttt tacaacagcc gttgggcgat
    31681 attgattata tccctgcaag caagcctaga cggctaccct ctgttatctc tgcaaatgaa
    31741 gtgcaacgca ttttgcaggt tatggatact cgcaaccaag ttatttttac gctgctgtat
    31801 ggtgcaggtt tgcgcattaa tgaatgcttg cgtttgcggg ttaaagattt tgattttgat
    31861 aatggctgca tcactgtgca tgacggtaag ggtgggaaaa gcagaaacag cctactgccc
    31921 acgcgcctaa tcccagcaat aaaataactc attgagcaag cgcggcttat tcagcaagac
    31981 gacaacttac aaggcgtagg gccatcgctg ccttttgctt tagatcacaa atacccttct
    32041 gcttatcgac aagcggcgtg gatgtttgtc tttccctcca gcacgctctg caaccacccg
    32101 tataacggca aattatgccg ccatcatctg catgactccg ttgcgcgaaa ggcattgaag
    32161 gcagccgtac aaaaagcagg catcgttagc aagcgtgtca cttgtcatac atttcgtcac
    32221 tcgtttgcta cgcatctatt acaagcgggg cgtgatattc gcactgtgca agaactctta
    32281 gggcataacg atgttaagac cacgcaaatc tatacgcatg tgttgggtca gcattttgcc
    32341 ggcaccacca gtcctgcgga tggactgatg ctacttatca atcagtaaaa gtagataaat
    32401 atctttatgt agccctgagt agcccctgat actttttatg gtgaaaaaga taaacaatca
    32461 tagaggggat aaatatgagc cagttctacc attgttcaga taagtttaag caagatactg
    32521 tcaatcacag tgaccattca tggtcattac acttcctcca gttgccagac aatgcctaac
    32581 ataaaaacgc aaaagcccaa aagtcagagc ttaggtgttc taggcgtttt ctaagcgctg
    32641 aagaaatacg tcttaccgta gattgcgtgc gtttctgaag cacattttcc ttatacatgg
    32701 ctttatccca ggcagcttca tcaagctctt ggagtaacaa gccagtaata atgcgacact
    32761 ctcgggtttg tagcgagccg ctggtgagat cggagttata gcggaaaggg gtcatttgta
    32821 attatttgtg ggtggaggat agttaactta ttattgtagt tggaagaggc tgctttccaa
    32881 acattagcac ctcgtaatgc ttgttactgt atattaattt atgctcttag cgcatgttaa
    32941 tgggctatta caatgcataa tgtccacatc aagataactg tggcttaaca cctgtacaag
    33001 cgaaaaaaat caatcacctg tccggaaata cgggatcact gtaaagtgtt ggtgaggcag
    33061 tctaggaggg ggatttaatt tgggatactg atgaaaggat tatgatcagt tggatatagg
    33121 atcgtactaa gctgagcggc aggttagcta gattagttaa ccagataagt gaaatctagt
    33181 tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc
    33241 atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt
    33301 tcccaactat tttgtccgcc c
//
LOCUS       DAJTRM010000039        33130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRM010000039 DAJTRM010000000
VERSION     DAJTRM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.039"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(2..94)
                     /locus_tag="OO322_001931"
     tRNA            complement(2..94)
                     /locus_tag="OO322_001931"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO322_001932"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO322_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5906832.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO322_001933"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO322_001933"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5906833.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO322_001934"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO322_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5906834.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO322_001935"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO322_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5906835.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO322_001936"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO322_001936"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5906836.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO322_001937"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO322_001937"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5906837.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO322_001938"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO322_001938"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO322_001939"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO322_001939"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5906838.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO322_001940"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO322_001940"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5906839.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO322_001941"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO322_001941"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5906840.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO322_001942"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO322_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5906841.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO322_001943"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO322_001943"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5906842.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO322_001944"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO322_001944"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5906843.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO322_001945"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO322_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5906844.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO322_001946"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO322_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5906845.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO322_001947"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO322_001947"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5906846.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO322_001948"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO322_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5906847.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO322_001949"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO322_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5906848.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO322_001950"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO322_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5906849.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO322_001951"
     CDS             complement(20900..21019)
                     /locus_tag="OO322_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5906850.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO322_001952"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO322_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5906851.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO322_001953"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO322_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5906852.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="OO322_001954"
     CDS             complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="OO322_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5906853.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGA
                     GSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKL
                     SNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMS
                     VSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFII
                     MAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSL
                     VPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="OO322_001955"
     CDS             complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="OO322_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5906854.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="OO322_001956"
     CDS             complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="OO322_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5906855.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26927..27277
                     /gene="hypA"
                     /locus_tag="OO322_001957"
     CDS             26927..27277
                     /gene="hypA"
                     /locus_tag="OO322_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5906856.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27281..28153
                     /gene="hypB"
                     /locus_tag="OO322_001958"
     CDS             27281..28153
                     /gene="hypB"
                     /locus_tag="OO322_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5906857.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28144..28416
                     /gene="hypC"
                     /locus_tag="OO322_001959"
     CDS             28144..28416
                     /gene="hypC"
                     /locus_tag="OO322_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5906858.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28416..29537
                     /gene="hypD"
                     /locus_tag="OO322_001960"
     CDS             28416..29537
                     /gene="hypD"
                     /locus_tag="OO322_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5906859.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29534..30544
                     /gene="hypE"
                     /locus_tag="OO322_001961"
     CDS             29534..30544
                     /gene="hypE"
                     /locus_tag="OO322_001961"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5906860.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30618..32696
                     /gene="flhA"
                     /locus_tag="OO322_001962"
     CDS             30618..32696
                     /gene="flhA"
                     /locus_tag="OO322_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5906861.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32733..33086)
                     /locus_tag="OO322_001963"
     CDS             complement(32733..33086)
                     /locus_tag="OO322_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5906862.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7806 a         9230 c         8624 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcagg ataatgccga tattttccag ggtgtggtaa
    24661 gccagcaggc gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg
    24721 atcatgccga gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt
    24781 gacagggtta acatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca
    24841 gctggcgcag aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatccca
    24901 gccagcaggc caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt
    24961 tgcatacgca tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag
    25021 caagcaatca ccagcagcag agtgccaaga cgcctcagcg caaaccacag tttgccctct
    25081 ttgctgttgc tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac
    25141 atgccgaggt tgctggcgat gacggcgcag acggcggcag ccatcaacat attgatctgc
    25201 aagccgttgc acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac
    25261 agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg
    25321 tagcttacca gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc
    25381 gcggctgccg tatacagact accaaccgcg ccgccaattc cggctatcca gccacttagc
    25441 gctttttgaa aagagaaatg ccagaacagc ggcggcgaca aaccatgcca cgccgctatt
    25501 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    25561 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    25621 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacacaggc cggaccttgt
    25681 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    25741 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    25801 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    25861 atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc cccatcaacg
    25921 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    25981 agctgcggcg cagattcttt ttcattgagc atcactctca ggcgcggcat tgattgcagg
    26041 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc gatacagagc
    26101 gtggagtcgg caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    26161 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    26221 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    26281 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    26341 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    26401 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    26461 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    26521 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    26581 ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    26641 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    26701 ttcccaaata gtcattgtca ggttactcgt ttaacagaga tgccagcttt aagcattttt
    26761 tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    26821 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    26881 tggcgcaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    26941 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    27001 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    27061 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    27121 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    27181 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    27241 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    27301 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    27361 cgcgccattt gccccggcgg cacgcccgca gatgaaaatc accggcatta aagcgcctga
    27421 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    27481 tcacacaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    27541 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    27601 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    27661 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
    27721 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
    27781 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    27841 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    27901 acacaaagta gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    27961 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
    28021 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    28081 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    28141 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    28201 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    28261 ggtcagccgc gcgtgggcca gtgggtactg gttcacgttg gctttgccat gagcgtaatt
    28321 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    28381 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    28441 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    28501 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgccattt
    28561 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    28621 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    28681 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggatcgc
    28741 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    28801 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    28861 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    28921 tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg ctggaacagc
    28981 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    29041 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    29101 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    29161 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctactg gcgcaacagg
    29221 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    29281 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    29341 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag gtattaacgg
    29401 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    29461 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    29521 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    29581 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    29641 agatcaggca cgtcttgatc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
    29701 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    29761 gatttgcggc accgcgaatg acgttgcggg cagtggcgct attccgcgct atctctcctg
    29821 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    29881 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    29941 gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    30001 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    30061 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    30121 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    30181 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    30241 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    30301 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    30361 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    30421 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    30481 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    30541 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    30601 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    30661 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    30721 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    30781 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    30841 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    30901 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    30961 ccaaatttgg tcactattgc ctgatgccac tggcggcggg agggcatatt tttggtggct
    31021 gtgaatttat tcgttatgac gagcgtccct ggagcgaaaa agagttcaat cgtctgcaaa
    31081 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg
    31141 tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt gccattacca
    31201 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    31261 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    31321 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    31381 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    31441 atctctacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    31501 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    31561 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
    31621 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    31681 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    31741 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    31801 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggctcgggta
    31861 aagagctgat tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    31921 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    31981 gtggggcttt taccggtgcc aacgcccagc gcattggtcg ttttgaactg gcggataaaa
    32041 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    32101 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    32161 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    32221 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    32281 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    32341 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    32401 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta acacgcggca
    32461 acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg
    32521 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    32581 aaaccaacag cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    32641 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    32701 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
    32761 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    32821 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    32881 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    32941 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    33001 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    33061 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatta
    33121 catcttaatc
//
LOCUS       DAJTRM010000040        31244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRM010000040 DAJTRM010000000
VERSION     DAJTRM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.040"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(419..625)
                     /locus_tag="OO322_001964"
     CDS             complement(419..625)
                     /locus_tag="OO322_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5906863.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            938..1027
                     /gene="kdpF"
                     /locus_tag="OO322_001965"
     CDS             938..1027
                     /gene="kdpF"
                     /locus_tag="OO322_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5906864.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1027..2700
                     /gene="kdpA"
                     /locus_tag="OO322_001966"
     CDS             1027..2700
                     /gene="kdpA"
                     /locus_tag="OO322_001966"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5906865.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2723..4770
                     /gene="kdpB"
                     /locus_tag="OO322_001967"
                     /pseudo
     CDS             2723..4770
                     /gene="kdpB"
                     /locus_tag="OO322_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4779..5351
                     /gene="kdpC"
                     /locus_tag="OO322_001968"
     CDS             4779..5351
                     /gene="kdpC"
                     /locus_tag="OO322_001968"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5906866.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5344..8024
                     /gene="kdpD"
                     /locus_tag="OO322_001969"
                     /pseudo
     CDS             5344..8024
                     /gene="kdpD"
                     /locus_tag="OO322_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8021..8706
                     /gene="kdpE"
                     /locus_tag="OO322_001970"
                     /pseudo
     CDS             8021..8706
                     /gene="kdpE"
                     /locus_tag="OO322_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8981..9085
                     /locus_tag="OO322_001971"
     CDS             8981..9085
                     /locus_tag="OO322_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5906867.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9396..11593
                     /gene="speF"
                     /locus_tag="OO322_001972"
                     /pseudo
     CDS             9396..11593
                     /gene="speF"
                     /locus_tag="OO322_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11590..12909
                     /gene="potE"
                     /locus_tag="OO322_001973"
     CDS             11590..12909
                     /gene="potE"
                     /locus_tag="OO322_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5906868.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12965..14605)
                     /gene="pgm"
                     /locus_tag="OO322_001974"
     CDS             complement(12965..14605)
                     /gene="pgm"
                     /locus_tag="OO322_001974"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5906869.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14631..15176)
                     /gene="seqA"
                     /locus_tag="OO322_001975"
     CDS             complement(14631..15176)
                     /gene="seqA"
                     /locus_tag="OO322_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5906870.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15361..16125
                     /gene="ybfF"
                     /locus_tag="OO322_001976"
     CDS             15361..16125
                     /gene="ybfF"
                     /locus_tag="OO322_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5906871.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16265..16558
                     /gene="ybfE"
                     /locus_tag="OO322_001977"
     CDS             16265..16558
                     /gene="ybfE"
                     /locus_tag="OO322_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5906872.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16698..17228
                     /gene="fldA"
                     /locus_tag="OO322_001978"
     CDS             16698..17228
                     /gene="fldA"
                     /locus_tag="OO322_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5906873.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17438..17524
                     /gene="uof"
                     /locus_tag="OO322_001979"
     CDS             17438..17524
                     /gene="uof"
                     /locus_tag="OO322_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5906874.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17517..17963
                     /gene="fur"
                     /locus_tag="OO322_001980"
     CDS             17517..17963
                     /gene="fur"
                     /locus_tag="OO322_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5906875.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18047..18373)
                     /locus_tag="OO322_001981"
     CDS             complement(18047..18373)
                     /locus_tag="OO322_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906876.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18410..19816)
                     /gene="chiP"
                     /locus_tag="OO322_001982"
     CDS             complement(18410..19816)
                     /gene="chiP"
                     /locus_tag="OO322_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5906877.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20395..22059)
                     /gene="glnS"
                     /locus_tag="OO322_001983"
     CDS             complement(20395..22059)
                     /gene="glnS"
                     /locus_tag="OO322_001983"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5906878.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22262..24208)
                     /gene="nagE"
                     /locus_tag="OO322_001984"
     CDS             complement(22262..24208)
                     /gene="nagE"
                     /locus_tag="OO322_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5906879.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24541..25341
                     /gene="nagB"
                     /locus_tag="OO322_001985"
     CDS             24541..25341
                     /gene="nagB"
                     /locus_tag="OO322_001985"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5906880.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25401..26549
                     /gene="nagA"
                     /locus_tag="OO322_001986"
     CDS             25401..26549
                     /gene="nagA"
                     /locus_tag="OO322_001986"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5906881.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26558..27778
                     /gene="nagC"
                     /locus_tag="OO322_001987"
                     /gene_synonym="nagR"
     CDS             26558..27778
                     /gene="nagC"
                     /locus_tag="OO322_001987"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5906882.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27826..28578
                     /gene="nagD"
                     /locus_tag="OO322_001988"
                     /pseudo
     CDS             27826..28578
                     /gene="nagD"
                     /locus_tag="OO322_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28975..30639
                     /gene="asnB"
                     /locus_tag="OO322_001989"
     CDS             28975..30639
                     /gene="asnB"
                     /locus_tag="OO322_001989"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5906883.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31018..31094
                     /locus_tag="OO322_001990"
     tRNA            31018..31094
                     /locus_tag="OO322_001990"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31052..31054,aa:Met,seq:cat)
     gene            31103..31187
                     /locus_tag="OO322_001991"
     tRNA            31103..31187
                     /locus_tag="OO322_001991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31137..31139,aa:Leu,seq:tag)
BASE COUNT         7446 a         7886 c         8138 g         7774 t
ORIGIN      
        1 attagatatg ttaacaatta attattctta atatatcaat aaagtatata ttactctctt
       61 tactatctgt ttttattgcg gtagcgatac taataacaaa attcggaatt tatttagatg
      121 ctggaattag aaaaagggat gacggctttc tcaccttacc ctgaagtttc acgaaagtgt
      181 gtaaggaagt atgcaatcta actggagatt tctgatgatc tagtcgctta gagcgacaat
      241 gaaggtgacc gcatcctggg tagccctatg aaaggatact attgaaacgt gatgctaaaa
      301 cagtgatgaa atgaataata cgatggaaac gtcttccatg ttaattatca tggaagacgt
      361 tgatgttgaa cacctaaacc agccccctga tacgaggctg gcttaagcgc gcaggcaatc
      421 agtaaaaatc accggttgcc ttttcggcct gatccatcca caccggttta tcgctggttt
      481 tcgaccagac gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga
      541 aaggcagcgc cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg
      601 caggatattc tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata
      661 gcaccggaaa aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta
      721 ctactcatcc gaccacttat ttttgcttat tgatggttta tttacattca cactgtaatt
      781 aaattacaca aaagttaaat taatactaac catcagttaa atcatggcct ttgccatttt
      841 tatacttttt ttacaccccg tccgcagatt tttgcgaaat ctttgcagcc agaattctac
      901 tcttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg tgatagccgg
      961 cgtgttgctg gtgtttttat tactgggcta tttggtttat gccctgatca atgcggaggc
     1021 gttctgatgg ctgcgcaagg gttcttactg attgccacgt ttttactggt attaatggtg
     1081 ctggcgcgtc ctttaggcag cgggctggcg cggctgatta atgacattcc tcttccaggt
     1141 acagcgggcg ttgaacgtat actctttcgt ttacctggcg tctctgacca tgagatgaac
     1201 tggaagcaat atctttgtgc cattctcggc ctgaacatgc tggggctggc ggtgctgttt
     1261 tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc ggggctgtcg
     1321 tgggatctgg cgctgaatac cgccgtcagc tttgtcacga ataccaactg gcaatcttat
     1381 agcggtgaaa ccacgttgag ctatttcagc cagatggcag gcttaacggt acaaaacttt
     1441 ctttctgccg ccagcgggat tgcggtgatt tttgccttca tccgtgcgtt tactcgccag
     1501 agcatgagca cactcgggaa tgcctgggtc gatctgctac gcatcacgtt atgggtgcta
     1561 gtccctgtgg cgttgttgat cgcactgttt tttattcaac aaggtgcgca gcaaaacttt
     1621 ctgccctatc aggcagtgaa taccgttgaa ggagcgcaac agctgttacc catgggacca
     1681 gtagcttctc aggaagcgat caagatgctc ggtactaacg gcggtggctt ctttaatgcc
     1741 aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca gatgctggcg
     1801 atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtgacggg cgatcgccgc
     1861 caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtt
     1921 atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcaga cagcagcatc
     1981 aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg
     2041 actacggcgg cgtcctgtgg cgcggtgatt gcgatgcatg attcgtttac cgctctcggc
     2101 ggcatggtgc cgatgtggct aatgcaaatt ggtgaagtgg tgttcggcgg tgtcggttct
     2161 ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt
     2221 ggtcgtacac cggaatatct gggtaaaaaa atagacgtac gcgagatgaa actgactgca
     2281 ctggcgattc tggttactcc aacactggta ctgatgggcg cggcgttggc gatgatgacc
     2341 gacgccggac gtagcgccat gcttaaccct agcccgcatg gttttagcga agtgctgtac
     2401 gccgtgtcgt ccgccgctaa caacaacggc agcgcctttg ccggattaag cgccaactct
     2461 ccgttctgga actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg ggtgattatc
     2521 ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc cagctccggc
     2581 acgctgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt
     2641 ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata tctctcctga
     2701 tgatattgag tgagcactga atatgagtcg taaacaactg gcgctattcg aaccaacact
     2761 tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa
     2821 tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta gcatcgcgat
     2881 ggcaagcggt gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg
     2941 ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca gtaaagcgca
     3001 ggccaacagt cttaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc gtgagccgaa
     3061 atatggcgct gcggcggaca aagttcctgc cgatcaactg cgtaaaggtg acatcgtgct
     3121 ggtagaagct ggcgatatta tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt
     3181 cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg gcggcgattt
     3241 tgcctccgtt accggcggca cgcgtattct ttctgactgg ctggtgattg agtgtagcgt
     3301 taaccccggt gagacatttc tggatcggat gatcgcgatg gtggaaggcg cacagcgacg
     3361 caaaacaccg aacgagattg ccctgactat cctgctgatt gctctgacca tcgtcttttt
     3421 actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgccg tgagtgtaac
     3481 ggtactggtg gcgctgctgg tctgtctgat cccaaccact attggtggcc tgttgtcagc
     3541 gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtgattgcca ccagcggacg
     3601 tgcagttgaa gcggcaggtg acgttgacgt tctgctactg gataaaaccg gcaccatcac
     3661 actcggtaac cgtcaggcgt cggagtttat ccccgcgcag ggcgtggatg aaaaaacgct
     3721 ggctgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggtc gcagtattgt
     3781 gatcctcgcc aagcaacgtt ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac
     3841 ctttgtaccg tttactgcgc aaagccggat gagcgggatc aacatcgaca accgcatgat
     3901 ccgtaaaggt tctgtcgatg ccattcgccg ccatattgag gctaacggcg gtcacttccc
     3961 tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcaa ggagccacgc tgctggtggt
     4021 ggtggaaggt tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat
     4081 taaagagcgc ttcgcccagc tgcgcaaatg ggcattaaaa cggtgatgat taccggcgat
     4141 aaccgtctga ctgccgccgc gatagctgcg gaagcgggtg tcgatgattt tctcgccgaa
     4201 gcgacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg tcgtttggta
     4261 gcgatgaccg gcgacagcac caacgatgct acggcgctgg cgcaggcaga tgtcgcggtg
     4321 gcgatgaact ccggcaccca ggcggctaaa gaggcgggca atatggtgga tctcgactct
     4381 aacccgacca agttgatcga ggtggtgcac attggcaaac agatgctgat gacccgtggc
     4441 tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat tattccggcg
     4501 gcgttcgcgg caacatatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc
     4561 gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt tttgattcct
     4621 ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac
     4681 ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat
     4741 ttactgctaa ccgtttgtag tctggtgtga ggtttaccat gagtggatta cgtccggcat
     4801 tatcgacatt tctctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg
     4861 cactggggca atggtggtat ccctggcagg ccaacggttc gttgattcgt gaaggtgata
     4921 cggtgcgcgg ttcggcatta atcgggcaaa attttaccgg caacggctat tttcatggtc
     4981 gcccgtcggc aacagcagaa atgccctata atccacaggc ttctggcggg agcaatctgg
     5041 cagtcagtaa cccggagctg gataaacaga tagccgcacg cgttgccgca ttacgggccg
     5101 ctaacccgga cgccagcacg aacgttccgg ttgaactggt gacggcgtcg gcaagcggac
     5161 tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcatc gcaaaagcgc
     5221 gtaatctcag cgttgaacag ctcacgcagc tgatcgcaaa atacagccaa caaccgctgg
     5281 tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca ctggataagc
     5341 ttgatgaata acgaaccctt acgtcccgac cccgaccgtc tgctggaaca aactgccgcg
     5401 ccgcatcggg ggaagctgaa agttttcttc agtgcctgtg caggcgtcgg gaagacctgg
     5461 gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt ggttggtgtg
     5521 gtagaaaccc atgggcggaa agataccgcc gccatgcttg aagggctggc tgttctgcct
     5581 ctgaaacgcc aggcgtaccg tgggcggcat atcagcgagt ttgatctcga tgccgccctc
     5641 gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc gccaggttct
     5701 cgtcatccta aacgctggca ggatatcgaa gaactgctgg aagctggcat tgatgttttc
     5761 acaaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg cgtcaccgga
     5821 attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga cgtggtgctg
     5881 gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt ctatattgcc
     5941 gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat cgccctgcgc
     6001 gaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc ctggcggggg
     6061 catcctggcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg catcggccat
     6121 aacaccggca gcgaaaactg gtccgcgcgg cggcgcggct gacgtcacgg ctggggagcg
     6181 tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg cttaccggag aaaaaacgtc
     6241 gggcaattct cagcgcctta cgtctggcgc aggagctggg cgcggagacg gcaacacttt
     6301 ctgatccagc ggaagagaaa gcggtagtgc gttatgcccg tgaacataat ctcggcaaga
     6361 ttattctcgg tcgcccagcc tcgcgccgct ggtggcgtcg ggaaacattt gctgaccgac
     6421 tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc
     6481 gcacgattaa caacgcgccg gatagccgct catttaaaga caagtggcgg gtacagattc
     6541 agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac cttaattgcc atgcagtggc
     6601 tgatggcgtt tgatgccgcc aacctggtga tgctgtatct gcttggcgtg gtggtggcgc
     6661 tattttatgg acgctggcct tcggtggttg ccaccgtcat taatgtggtg agtttcgatc
     6721 tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat ctgctgacct
     6781 tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgctggt gtgcgttatc
     6841 aggcgcgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa atgtcgaaag
     6901 ctctggcggt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa tttattgcct
     6961 ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa ttgcagccgt
     7021 taacacatcc gcaaggaatt acgccatggg acgatgccat cgcgcagtgg agttttgata
     7081 aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg tgtaccgtac cagattttgc
     7141 cgctaaaaag cggcgagaaa acctacgggc tggtggtggt ggagccgggg aatctgcgcc
     7201 agttgatgat cccggaacag cagcgcctgc tggagacttt tacgctgtta gtcgccaatg
     7261 cccttgagcg gctgacgcta accgccagcg aagaacaggc gcggatggca agcgaacgtg
     7321 aacagatccg caacgccctg ctggcagcgc tttcgcatga tttacgcacg ccgcttacgg
     7381 tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga tcaccccacg
     7441 cccgccaggc cagcgagatt cgtcagcatg tgctgaacac tacccgactg gtgaataatc
     7501 tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag tggttaacgc
     7561 tggaagaagt agtcggcagc gcgctgcaaa tgccggaacc gggtttatcg tcgcccatca
     7621 atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccactc tttgaacggg
     7681 tgctgattaa tctgctggag aacgcggtga aatatgcggg tgcgcaggcc gaaattggta
     7741 tcgatgccca cgttgagggc gaaaatctac aactggatgt ctgggataac ggccccggtt
     7801 ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat aaagagtcgg
     7861 cggtgccggg tgtcgggctt gggttggcaa tttgtcgggc gattgtggat gtacacggcg
     7921 gcaccattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc
     7981 agcaaaccgc tcctgaactt gaagactttc atgaggatat gtgacaaacg ttctgattgt
     8041 tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg acgggatgcg
     8101 cgtctttgag gccgaaactc tgcaacgcgg cttgctggaa gcggcaaccc gtaagccaga
     8161 tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct
     8221 gcgccagtgg agcccggtgc cggtgattgt gctttccgca cgcagcgaag agagcgacaa
     8281 gatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg gtattggcga
     8341 attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc gccactccgc caccgccgcg
     8401 cccgatccgc tggtgaaatt ttccggtgtt accgtcgatt tagccgcccg cgtgattcac
     8461 cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctggc ggtgctgctc
     8521 aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg ggggccaaac
     8581 gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca aaaactggaa
     8641 caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta tcggtttatg
     8701 ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat taaacctgcg
     8761 ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca cgaaattaca
     8821 acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg atttattatc
     8881 tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc atggatagaa
     8941 taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata ataaccgctt
     9001 aatgcctcat ataaggcgga caacccatat catgaagttt acccatcgta atagttttga
     9061 ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac gtacaggtct
     9121 ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata tactgctaac
     9181 ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat gacgagtata
     9241 gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac tgatttattt
     9301 atcagcggtg gagctttgct ttttttccga cgtgatcgat ttctcctttg agaaattgag
     9361 gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa attgccgtta
     9421 gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt gacgaaagta
     9481 attatattga cgttgccgcc attattttat cagtcaatga tgttgaacgt ggaaaactcg
     9541 ataaaataga cgctactggc tatggcattc ctgtttttat tgcaacggaa aatgaagaac
     9601 gtgtaccagc agaatatctg ccacgtattt ctggtgtctt cgagcattgc gaatcacgga
     9661 aagagtttta tggacgtcag ttagaaaccg ctgctagcca ttatgaaacg caactgcgcc
     9721 cgcccttctt ccgcgcactg gtcgattatg tcaatcaggg caatagcgct tttgactgcc
     9781 cgggtcatca gggaggtgag tttttccgtc gtcatccggc agggaatcag ttcgtggagt
     9841 acttcggaga aacgctgttc cgttccgact tatgcaacgc agacgtagca atgggcgatc
     9901 tgctgattca cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa gtgtttaatg
     9961 ccgataaaac atacttcgtt ctaaatggca cttcatcttc taacaaagtg gttttaaacg
    10021 ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa taaccacaaa tctaaccacc
    10081 acggagcgtt gctacaggct ggtgcaacac cggtttatct ggaaacggca cgtaacccgt
    10141 atggctttat cggtggcatt gatgcgcact gttttgaaga aagttacctg cgtgagctga
    10201 tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc gctgtgattc
    10261 agttaggcac ctacgacggt acgatttata acgcccgcca ggtggtggat aaaattggtc
    10321 atctgtgtga ctacattctg tttgactccg catgggttgg ctatgagcaa tttatcccga
    10381 tgatggctga ctgttcgccg ctgttgctgg atcttaatga gaacgatccg ggtattctag
    10441 ttacgcaatc cgtacataaa cagcaggctg gtttttctca aacttcacaa atccataaaa
    10501 aagacagcca catcaaaggg caacagcgtt atgtaccgca caaacgcatg aacaacgcct
    10561 ttatgatgca cgcctccacc agcccgttct atccgctgtt tgccgcactg gatatcaacg
    10621 ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg gtaaatggca
    10681 ttaatgcccg caaactgatc ctcgataact gtcagcatat tcgtccgttc gtacctgaac
    10741 tggtggatgg taaaccctgg cagtcgtatg aaacagcgca aattgcggtt gatctgcgct
    10801 tcttccagtt tgtaccaggg gaacactggc attcttttga aggctatgca gagaatcaat
    10861 actttgtcga tccttgcaaa ctgttgctga caaccccagg tattgatgca cgtaacggcg
    10921 aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc gaaaatggcg
    10981 tagtgccgga aaaatgcgat cttaacgcca tcctcttcct gctgactccg gcagaagata
    11041 tggccaaact tcagcaactt gtgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    11101 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11161 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11221 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11281 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctac cggatgccga
    11341 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11401 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11461 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11521 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11581 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11641 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11701 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11761 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11821 tatgctttcg gtaaatccgg taactttatt gcgaactata cctacggcgt ctcactgctg
    11881 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11941 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    12001 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtgggggggc
    12061 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12121 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12181 atgacgctgt gggcttttct tggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12241 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12301 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12361 acggcaccat ttggcctggc cttcgcacag atgtttacac cagaagtggg taaagtcatt
    12421 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12481 caggtgttta aatcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12541 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12601 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12661 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12721 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12781 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12841 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12901 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgctctttt tttattaaat
    12961 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    13021 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    13081 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgcca atagaagcac
    13141 cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc acctgccagg gtgctggcgc
    13201 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13261 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13321 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacataatg atgccgtctt
    13381 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13441 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggta
    13501 cttctaccag cttacggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13561 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13621 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13681 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt gccagcagac
    13741 ccgccatcgc acactcggag gagcagtcca tacggatcgc accgtcttta tcgaggtgca
    13801 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13861 cgccaatgcg cttccagtat tcgataccgg aaccgcccaa cggatcaacg cccagcgtca
    13921 ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc cagcccttcc acgaacggct
    13981 gcaccagatc ctgctctttc acatagccgg atgccatcgc ttcgtcgagg gagatacgct
    14041 tcacgccttt caggccatcg gccatcagtg cgttggcctt gtcttccacc actttggtga
    14101 cgttagtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14161 ggttatggga cggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattgtgaa
    14221 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14281 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14341 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14401 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14461 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14521 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14581 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14641 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14701 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14761 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtac
    14821 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14881 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14941 tccgaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    15001 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    15061 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15121 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15181 atccagtgca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15241 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15301 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15361 atgaaattaa atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15421 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15481 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagagccg
    15541 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ccctggatgc actgcagatc
    15601 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttactgca
    15661 ctggcccccg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15721 gtacgccgtc atgatgagat tttcgcagct atcaacgcgg tcagtgaatc ggacgcacaa
    15781 actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg ggtgattcag
    15841 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15901 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15961 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    16021 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    16081 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16141 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16201 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16261 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16321 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16381 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16441 ggaagctgtc gaggcgctga acgagctggc ggaatcgcgt aatatgagcc gtagcgaact
    16501 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16561 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16621 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16681 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16741 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16801 acattgcaaa aagcagcaaa gaagatctgg aagcttacga cattctgctg ctgggcatcc
    16861 caacctggta ttacggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16921 agattgattt caatggtaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16981 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    17041 ccatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggcag
    17101 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17161 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctta
    17221 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17281 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tatacaatgt cccagtgttc
    17341 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17401 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17461 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17521 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17581 tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17641 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17701 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17761 tgacacagca acatcaccac gatcacctga tttgcctcga ctgcggcaag gttatcgaat
    17821 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17881 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17941 agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa aagccaacct gcgggttggc
    18001 ttttttatac aagggaaata agaaaagcga gagttacagc tctcacttat ttgttattac
    18061 tgcgtatttc ctgccagacc ttatcacaat cggctttcac cgcctgatca tttccggttt
    18121 gcgttgcacg cagacactgc tgataatcca gtacgcgtac actttcctgg tcggcaaact
    18181 gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct tcaatttttt
    18241 ctggcgaacc ttgcgcggtg ttgatacagg cgctgtatgc ctctttcaga cggctgtctt
    18301 cctgtggtgc ggttgattgc gcacaagcta ccagccccga tgccatcatg gcgatgagaa
    18361 tcagtttttt cattgaatgc ggcaggcaca aaacctgccg cgtcgggcat cagaagatgg
    18421 tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg ttaccgtaac
    18481 cgccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc agtttgaaca
    18541 tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg taggcagact
    18601 cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag gtcgcaggtt
    18661 tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga ttccagtttt
    18721 tcaggtcata catcgcactg aagaagaccg ctttttcgcc gttagcgttg aagtcggaac
    18781 ggttatccca ccagatatcc aggcggccgt tggaggaagc gtaggttggg gtcatacgtt
    18841 gcaggaagta tccctgctga ctgtcagcct taatccaggt gccttcgagg cgcaaatcca
    18901 ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg ccgtcataaa
    18961 gatcgttgac gctgcgatcg tcaactttat cgcgggtacc atagaactgg tagctggtgg
    19021 ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat tgatcgatat
    19081 acccttccgc ctgaccaaat gccgcttcca gtacgaagtt atttttgaag tcgtatttcg
    19141 caccgatgga gtgcagataa tcaactttgg tggttttatc gttctgataa aactcatcca
    19201 tttccagatg ccacggcgct ttgtattcgt tggtccacat gtaggagaaa ctcaaggcac
    19261 cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta cccggcataa
    19321 agctccagtg cggcgctaac agcgtttgac cggttggctg aatgtaacct gccctcgccc
    19381 aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg cttttgtcgc
    19441 cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg ttcgggtggg
    19501 agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc agcaatatca aggccgaaca
    19561 tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt
    19621 tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc tgccagtaat
    19681 agataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg
    19741 ccataaagcc cgacattgct gtaacaccgg caatagccag cgccagcgta ctacgtttgc
    19801 cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac caaaggtgaa
    19861 cagcgaaaaa aaataaaaat taaaaaagta gcgttgagaa aagtctcgct gactacagga
    19921 acagactatt tttcgcgacg cgaattatca atctctttct gtaaccagaa tgtaagaaca
    19981 tgagcaaccg cacatatttt gatgattttt gttacaaggc ttaagtttgc ggttgaaagg
    20041 cttgcagaag aataaaaacg tgaaattcca gcaagatgaa agcggttaca ttttatttaa
    20101 ttatgttttg tgattttaaa tttattttat ggataaattg cgaagcggaa ttaattcgcg
    20161 aagaaaagca gaaaaaacgc cgcagagcgc ggcgtttggg attgtttgct atttcttgcc
    20221 ggatgcggcg tgaacgcctt atccggccta caaaagcacg caaattcaat atattgcaga
    20281 gatcatgtag gcctgataag cgaagcgcat caggcaattt agcgtttgaa atttattgtc
    20341 tatttattgc cggatgcgac gtaaatgcct cacccggctt ggtagaactt aaaattactc
    20401 gcctactttc gcccaggtat cacgcagccc aacggtgcgg ttaaataccg gtttttcggc
    20461 cgtggaatgg cggctgtcga ggcagaagta accttcacgc tcaaactgga atgctttacc
    20521 cgcaaccgca tctttcagcg acggttcagc aaagccctgt ttgatcacca gcgattccgg
    20581 gttaatcacc gacaggaaat catccgcagc acctgggtta ggcacgctga acagacgatc
    20641 atacaaacgg atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa taacaccttt
    20701 gactttacga ccatctgccg gatctttgct taaggtgtcg gcgtcataag tacagaagat
    20761 ggtggtgata ttaccttcgg catctttctc cacgcgttca gccttaatga cgtaagcatt
    20821 acgcagacgc acttctttac ccagcaccag acgtttgtac tgcttgttag cttcttcgcg
    20881 gaaatcggcg cgatcgatcc aaatttcacc gctaaacggc acctgacggc tccccatttc
    20941 cggtttgttc ggatggttcg gcatggtgac catttcgcct tcgccctgat agttttcgat
    21001 aaccagtttc accggatcga taaccgccat tgcgcgcggc gcattttcgt tgagatcttc
    21061 acggatgcag gattccagcg acgccatctc aatggtgttg tcctgcttgg tcacgccgat
    21121 gcgtttgcag aactcacgaa tagaagccgc agtgtaacca cgacgacgca gaccggaaat
    21181 ggtcggcata cgcgggtcat cccagccttc aacgtgcttg tcggtcacca gcaggttcag
    21241 cttacgcttg gacatcacag tgtattccag attcaggcgc gagaattcgt actggcgcgg
    21301 atgaacagga atggtgatgt tatccagtac ccagtcgtac agacgacggt tgtcctggaa
    21361 ctcaagcgta cacagagagt gcgtaatacc ttccagcgca tcgctgatgc aatgggtgaa
    21421 gtcgtacatc gggtagatgc accacttgtt gccagtctgg tggtgttcag caaatttaat
    21481 gcggtacagc accggatcgc gcattacgat aaatggcgac gccatgtcga ttttcgcacg
    21541 caggcaggct ttaccttctt caaaaccacc ggcacgcatt ttttcgaaca gcgccaagtt
    21601 ctcttcaacg ctgcggtcgc ggtacgggct gtttttaccc ggctgcgtca gggtaccgcg
    21661 gtattcgcgg atctgttccg gtgtcagttc atcaacgtac gccaggcctt tattgatcag
    21721 ttcgatcgca taggcgtgga gctgatcaaa ataatcggag gagtaacgga cgttaccaga
    21781 ccagtgaaaa cctaaccact ctacgtcgtt tttgatcgaa tcgacatact cgatatcttc
    21841 ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt cctgggcgat
    21901 cccgaagttc aggcagatag atttcgcatg gccaatatgc agatagccat tcggctccgg
    21961 cgggaaacgg gtgtgtactg tgatgtgctt accactggcc agatcttcat cgatgatctg
    22021 acggataaag ttagtcgggc gggcttctgc ctcactcatc gtggattcct caaagcgtaa
    22081 acaacgtata acggcgtatg atcttataag cgggacaggc tgacaactgt tagtttttta
    22141 aaattgataa tctaacaagg attatgggga tgattgatga aaaaaaaagc ggcagagaag
    22201 atctccaccg cttgtcattg ttggatgcga cgctgccgtg tcgcatcagg cataaagcag
    22261 attacttttt gatttcatac agcggtgttt gacccgccac aacatggccc tgagctttaa
    22321 tgagcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc atcgagcggg
    22381 cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg cttacctgcg
    22441 caccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg tcgataccca
    22501 tatggacgac gatctccgcg cctttttcgg tttccaggca gaacgcgtgg ttggtgttga
    22561 agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc ggtttcaccg
    22621 ccacaccgtc acccaccgct ttgctggcga atgcttcgtc aggaacctga tccagtgcca
    22681 cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt ggtacagcct
    22741 gcggttttgc tacaggcgcg gcagttgccg gagttgcttc agctgacgca gcggctaccg
    22801 gaccacgggc aacgactttc ttcatcgcat cgccgatgga ttctgctttc gcgccaacaa
    22861 tcacctgaat agtctgtttg ttcagtttca ctacaccaga agcacccaga cgtttacaca
    22921 tcgtatcgtt gacgcgggca gagtcagcca cggtcaggcg cagacgggta atacaggcat
    22981 caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt gccagttgag
    23041 tcagaccttc ttcagtattg ctgttggctt cttcagtaac gatctcgtct tctttatctt
    23101 cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc acgaagtaga
    23161 tagcgaagaa gataacgccc atcaccagca gcatccagac gttctggctg gcggcaggca
    23221 ggttatacat caacgcgtag tcgatagcac ccgcagagaa agagaagccg gcatggatac
    23281 ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg aggtacagca
    23341 gcggcgcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc aggaacgcag
    23401 taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc ggtgctgcga
    23461 agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag aagccggaca
    23521 tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg tggaaaaccg
    23581 tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc agcacctgat
    23641 gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa cccagcgcgc
    23701 ccgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtaccggc ggccagacgt
    23761 aaccaaaaat ggccgccagc accagacaga agaagccggt ggcgatcggc acaaagcgtt
    23821 tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg ttataggctg
    23881 cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt tctgggttga
    23941 tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc gccgccgcac
    24001 ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat gcgaggttat
    24061 caaaaattgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct ggctgaccga
    24121 atcgcagcaa cagcgccgcc accggcagca ccgcgatagg gagctgtaac gccctaccga
    24181 gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc tatttggctc
    24241 gtttaaagcc gtatttttat tttgctgcaa attttactgc cgatgttctg taatcagatt
    24301 gttagatcat ctgctacaga gtgtgtgaaa atttaattcg catcgcaaat taaacgcgtg
    24361 tcttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact ggctaaacca
    24421 gaaaacttat tttatcattc aaaaaatcaa gtcggattga cgcctgtctg cgcaaatcca
    24481 ggttacgctt aaagatgtct aatccgccaa cggcttacat tttacttatt gaggtgaata
    24541 atgagactga tccccctgac taccgctgaa caggtcggca aatgggctgc tcgccatatc
    24601 gtcaatcgta tcaatgcgtt caaaccaact gccgatcgtc cgtttgtact gggcctgccg
    24661 actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa agcaggccag
    24721 gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct gccgaaagag
    24781 catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt tgatattcca
    24841 gcagaaaaca tcaaccttct caacggcaat gccccggata tcgatgccga gtgccgccag
    24901 tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg tgtaggtaac
    24961 gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac tcgtatcaaa
    25021 accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga tgtcaatcag
    25081 gtgccaaaat atgccctgac tgtcggtgtt ggtacactgc tggatgccga agaagtgatg
    25141 attatggtgc tgggtagcca gaaagcactg gcgctgcagg ccgccgttga aggttgcgtg
    25201 aaccatatgt ggaccatcag ctgtctgcaa ctgcatccga aagcgatcat ggtgtgcgat
    25261 gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga attagaagca
    25321 gaaaatatca aaggtctgta attgttatcc ctgccctggt tccttgctca gggcaatatt
    25381 ttttaaaatc ggggggcaga atgtatgcat taacccaggg ccggatcttt accggccacg
    25441 aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc gtctgtccgg
    25501 tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt ctctcccccg
    25561 gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac accgctgaag
    25621 cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca ggctgtacta
    25681 actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc gtgcgcgtta
    25741 tgcgtgagta cctggcaaaa catccgaatc aggcgttagg tctgcatctg gaaggtccgt
    25801 ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag cctgatgccg
    25861 cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc ctggcaccgg
    25921 aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg gtttctgccg
    25981 gtcactccaa cgcgacgttg aaagaagcaa aagccggttt ccgcgcgggg attacctttg
    26041 ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc ctggcgggcg
    26101 cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg catgttgatt
    26161 acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg gttaccgacg
    26221 ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa acaatatact
    26281 accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc ttaaccatga
    26341 ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa gtgctgcgta
    26401 tggcgacgct ctatccggcg cgtgcgattg acgttgaaaa acgtctcggc acgctcgccg
    26461 caggtaaagt agccaacctg accgcattca cacctgattt taaaatcacc aagaccatcg
    26521 ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg gacaagctca
    26581 gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc gcctgattga
    26641 ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg cccccgccag
    26701 cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag ttgatcagca
    26761 ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca atttccacgc
    26821 aatcggcgta cggcttggtc gtcatgacgc caccatcact ctgtttgatc tcagcagcaa
    26881 agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcagacgc tggaacatgc
    26941 cctgctgaat gccattgctc agtttattga tagctaccag cgcaagctgc gcgagctaat
    27001 cgccatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa ttcattacat
    27061 gccgcatatt caggtagaaa actgggggct ggtagaagca ctggaagaac gttttaaagt
    27121 gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact acttcggtgc
    27181 aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg gggccgggat
    27241 tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa ttggccatat
    27301 tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc tggaaactat
    27361 cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg gctaccagag
    27421 ccgtgtgcca ctggacgact gcaccatcaa aactatctgc aaagccgcga acaaaggcga
    27481 tagcctggcc tcggaagtga ttgagtatgt cggtcgtcat ctgggtaaaa ccatcgccat
    27541 tgctatcaac ttatttaatc cgcaaaaaat tgttattgct ggtgaaatca ccgaagccga
    27601 taaagtgctg ctccctgcta ttgaaagctg cattaatacc caagcgctga aggcgtttcg
    27661 tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg gcgcttttgc
    27721 gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg aaaattaatg
    27781 tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac cattaaaaat
    27841 gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc gggtgcagcg
    27901 gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac caactatcct
    27961 tcgcagactg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga tgtgccggat
    28021 agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcta ggaaggcaag
    28081 aaagcgtatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc cggtttcact
    28141 attaccgatg tgaaccctga tttcgtgatt gttggcgaaa cacgttccta caactgggac
    28201 atgattcata aagcagccta tttcgtcgct aacggtgcac gctttatcgc caccaacccg
    28261 gacacccacg ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg gattgagaaa
    28321 atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat ccgcgcggca
    28381 ttaaacaaaa tgcaggcgca ttcggaagaa acagtgattg tcggcgataa cctgcgtacc
    28441 gatattctgg cgggcttcca ggccggtctg gagacgattc tggtgctttc tggtgtttca
    28501 tcgctcgacg atatcgacag tatgccgttc cgcccaagct ggatttaccc gtcagtcgct
    28561 gaaatcgacg ttatctgaat accacccacg tctgcggacg tggttggctc tgatgacaaa
    28621 cgccaaactg cctgatgcgc tacgcttatc aggcctacgc agctcctgca atatattgaa
    28681 ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaatcgc
    28741 actttatcag caatttggcc cccgtaaaat ataaccattc aataaaaccc gctaaaaatt
    28801 atcgattcat caataaatac atcctcacac cagacccttt ttcaccaatc gacaatcacc
    28861 attacgtttt ttatttttgc actgccaaat agcttgcgca cagtgctgct ttgcggcatt
    28921 tttttaaaca agcaaacaca acaagcaaca aataccaggt taacggagaa ggttatgtgt
    28981 tcaatttttg gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa gaaagcactc
    29041 gagctgtcac gcctgatgcg tcatcgtggc ccggactggt ccggtattta tgccagcgat
    29101 aacgccattc tcgctcacga acgtctgtca attgttgacg ttaacgcggg ggcgcaacct
    29161 ctctacaacc aacaaaaaaa ccacgtactg gcggtaaacg gtgaaatcta caaccaccag
    29221 gcattgcgcg ctgaatatgg cgatcgttat cagttccaga cagggtctga ctgtgaagtg
    29281 atcctcgcgc tgtatcagga aaaagggccg gaatttctcg acgacttgca gggcatgttt
    29341 gcctttgccc tgtacgacag cgaaaaagat gcctacctga ttggtcgcga ccatctgggg
    29401 atcatcccac tgtatatggg gtatgacgaa cacggtcagc tgtatgtggc ctcagaaatg
    29461 aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc cggcggggag ctatttgtgg
    29521 agtcaggacg gcgaaatccg ttcttattat catcgcgact ggttcgactt tgatgcagtg
    29581 aaagataacg tgaccgacaa aaacgagctg cgtcaggcac tggaagattc cgttaaaagc
    29641 catctgatgt ctgatgtgcc ttacggtgtg ctactttctg gtggtctgga ttcctcaatt
    29701 atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg aagatcagga acgctctgaa
    29761 gcctggtggc cgcagttaca ctcctttgct gtaggtctgc cgggttcacc ggatcttaaa
    29821 gcagcccagg aagtggcaaa ccatctgggc acggtgcatc acgaaattca cttcactgta
    29881 caggaaggtc tggatgccat ccgcgacgtg atttaccaca tcgaaactta tgatgtgacc
    29941 actattcgcg cttcaacacc gatgtattta atgtcgcgta agatcaaggc gatgggcatt
    30001 aaaatggtgc tgtccggtga aggttctgat gaagtgtttg gcggttatct ttacttccat
    30061 aaagcgccca acgttaaaga actgcatgaa gagacggtgc gtaaactgct ggccttgcat
    30121 atgtatgact gcgcgcgcgc caacaaagcg atgtcagcct ggggcgtgga agcacgcgtt
    30181 ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca ttaacccgca ggataaaatg
    30241 tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat gttttgagtc atacctgccc
    30301 gcaagcgtgg cctggcggca gaaagagcag ttctccgatg gcgtcggtta cagttggatc
    30361 gacaccctga aagaagtggc ggcgcagcag gtttctgatc agcaactgga aactgcccgc
    30421 ttccgcttcc cgtacaacac gccgacctca aaagaagcgt atctgtaccg cgagatcttt
    30481 gaagaactgt tcccacttcc gagtgccgct gagtgcgtgc cgggcggtcc ttccgtcgcg
    30541 tgttcttcag ctaaagcgat cgaatgggat gaagcgttca aaaaaatgga cgatccgtct
    30601 ggtcgtgcgg ttggcgttca ccagtcggcg tataagtaag aaatgcggta ctgccccgga
    30661 gaaatccggg gctgttttag ttattgcgct gcttgttgat tctgatatgc agtaaggaaa
    30721 tgttccgggt ttgccacatc actgtttgac aactcatcac tgtaggccag tatttcatag
    30781 attaatttcc caccactatt gttgaattcc gaatttcatc ctgtacgaca aaagaatggc
    30841 tgtagggtgg ggaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc
    30901 gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca
    30961 aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaagatggc
    31021 tacgtagctc agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc
    31081 cgtcgtagcc accatctttt ttgcgggagt ggcgaaattg gtagacgcac cagatttagg
    31141 ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc accagaaagc
    31201 gttgtacgga tggggtatcg ccaagcggta aggcaccggt tttt
//
LOCUS       DAJTRM010000041        30855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRM010000041 DAJTRM010000000
VERSION     DAJTRM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.041"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            58..1716
                     /locus_tag="OO322_001992"
     CDS             58..1716
                     /locus_tag="OO322_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5906884.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1713..2669)
                     /locus_tag="OO322_001993"
     CDS             complement(1713..2669)
                     /locus_tag="OO322_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5906885.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2820..3935
                     /gene="macA"
                     /locus_tag="OO322_001994"
     CDS             2820..3935
                     /gene="macA"
                     /locus_tag="OO322_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5906886.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3932..5878
                     /gene="macB"
                     /locus_tag="OO322_001995"
     CDS             3932..5878
                     /gene="macB"
                     /locus_tag="OO322_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5906887.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5951..6175)
                     /gene="cspD"
                     /locus_tag="OO322_001996"
     CDS             complement(5951..6175)
                     /gene="cspD"
                     /locus_tag="OO322_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5906888.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6498..6818
                     /gene="clpS"
                     /locus_tag="OO322_001997"
     CDS             6498..6818
                     /gene="clpS"
                     /locus_tag="OO322_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5906889.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6849..9125
                     /gene="clpA"
                     /locus_tag="OO322_001998"
     CDS             6849..9125
                     /gene="clpA"
                     /locus_tag="OO322_001998"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5906890.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9528..9615)
                     /locus_tag="OO322_001999"
     tRNA            complement(9528..9615)
                     /locus_tag="OO322_001999"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9579..9581),aa:Ser,seq:gga)
     gene            complement(9869..10087)
                     /gene="infA"
                     /locus_tag="OO322_002000"
     CDS             complement(9869..10087)
                     /gene="infA"
                     /locus_tag="OO322_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5906891.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10372..11076)
                     /gene="aat"
                     /locus_tag="OO322_002001"
     CDS             complement(10372..11076)
                     /gene="aat"
                     /locus_tag="OO322_002001"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5906892.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11118..12839)
                     /gene="cydC"
                     /locus_tag="OO322_002002"
     CDS             complement(11118..12839)
                     /gene="cydC"
                     /locus_tag="OO322_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5906893.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12840..14606)
                     /gene="cydD"
                     /locus_tag="OO322_002003"
     CDS             complement(12840..14606)
                     /gene="cydD"
                     /locus_tag="OO322_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5906894.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14729..15694)
                     /gene="trxB"
                     /locus_tag="OO322_002004"
     CDS             complement(14729..15694)
                     /gene="trxB"
                     /locus_tag="OO322_002004"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5906895.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16238..16732
                     /gene="lrp"
                     /locus_tag="OO322_002005"
     CDS             16238..16732
                     /gene="lrp"
                     /locus_tag="OO322_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5906896.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16867..20895
                     /gene="ftsK"
                     /locus_tag="OO322_002006"
     CDS             16867..20895
                     /gene="ftsK"
                     /locus_tag="OO322_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5906897.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21050..21661
                     /gene="lolA"
                     /locus_tag="OO322_002007"
     CDS             21050..21661
                     /gene="lolA"
                     /locus_tag="OO322_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5906898.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21672..23015
                     /gene="rarA"
                     /locus_tag="OO322_002008"
                     /gene_synonym="mgsA"
     CDS             21672..23015
                     /gene="rarA"
                     /locus_tag="OO322_002008"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5906899.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23106..24398
                     /gene="serS"
                     /locus_tag="OO322_002009"
     CDS             23106..24398
                     /gene="serS"
                     /locus_tag="OO322_002009"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5906900.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24638..27081
                     /gene="dmsA"
                     /locus_tag="OO322_002010"
                     /pseudo
     CDS             24638..27081
                     /gene="dmsA"
                     /locus_tag="OO322_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27092..27709
                     /gene="dmsB"
                     /locus_tag="OO322_002011"
     CDS             27092..27709
                     /gene="dmsB"
                     /locus_tag="OO322_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5906901.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27711..28502
                     /locus_tag="OO322_002012"
     CDS             27711..28502
                     /locus_tag="OO322_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5906902.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28538..29164)
                     /locus_tag="OO322_002013"
     CDS             complement(28538..29164)
                     /locus_tag="OO322_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5906903.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29479..30627
                     /locus_tag="OO322_002014"
     CDS             29479..30627
                     /locus_tag="OO322_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5906904.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7585 a         7594 c         8304 g         7372 t
ORIGIN      
        1 ggcctttccc gttatactgc gagcgtaaag gataagtcac atatttctgg aggggatatg
       61 attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctc
      121 gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc
      181 ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg
      241 ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttccgc
      301 gaatcgctgc caggccgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg
      361 ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac
      421 ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat tgatgttgag
      481 gatattaacg atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat
      541 gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa tgtggaagtc
      601 accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca aaatctctct
      661 gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca ttatttctct
      721 gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa
      781 cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg agggcgctcg
      841 tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg cacattgcga
      901 ctggataaag acgcgcgtcc gttattgcta attgaagatc cagaaacccg cctgcatccg
      961 attatgcttt ccgttgcctg gcatctgttg aatcttttgc ctttacagcg tattgctacc
     1021 accaactctg gcgagctgct ttcgttaact ccagtggaac atgtttgccg cctggtgcgt
     1081 gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac cgaagacagc
     1141 cgccgcatct cttttcacat tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg
     1201 ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga
     1261 catcattttg atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca
     1321 ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa
     1381 gcagggaaga aatatgccgc tacggtacgc agcctgttga ataacgatcg ggaagccgaa
     1441 cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga
     1501 ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat gaatctacgc
     1561 aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca
     1621 atggaggcag gacgtcgtgg tgtggactcc gtaccaacgc tgctgaaaaa aatgttctca
     1681 cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt
     1741 ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg
     1801 atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca
     1861 catacgccgc caaccgactc ccagaacgcg ttgtaatcag catggatctt gccttcttta
     1921 tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct
     1981 acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt gggaaatagc
     2041 ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct
     2101 tgcagtccgc caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatc
     2161 tctgccagag gaataccttc gctgttgcgg aacaggattg tgctgtcacc ttctttatcc
     2221 attgagatca tcatggtcag ctcaagcgtg aactgctcac cgtttttgcc ttccagttta
     2281 gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga catacacccg
     2341 cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcg
     2401 gcaaggtaag ggcgatgcag acgcacaggc agtcgcggct ggcgcgttaa caacacattg
     2461 agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac
     2521 gagcgcagca aaaatttctg gcgaaaacta cagcgatgcc agaatttacc cggccgacac
     2581 tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga tggcgtaaag
     2641 gtccgttcag ttagctgcga catattcatg aaatcaatgg ttatacatga cgtcgatttc
     2701 accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat tttagggttt
     2761 tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta
     2821 tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg
     2881 gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg
     2941 tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta
     3001 aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg
     3061 ataaagtaaa aaaagaccag cttttagggg tcattgatcc tgaacaggct gaaaaccaga
     3121 tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg
     3181 agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaagcgg
     3241 tttcactgca ggatctcgat accgccgcga cggagatggc tgtgaaacag gcgcaaattg
     3301 gcaccattga cgcgcaaatc aagcgcaatc aggcctctct cgatacggct aaaaccaatc
     3361 tcgattacac ccgtatcgtt gccccgatgg ctggggaagt gacgcaaatc accactctgc
     3421 aaggccagac ggtgattgcc gcacagcaag caccgaacat tctgacgctg gcagatatga
     3481 gcactatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc
     3541 aaaaagcctg gtttacggtg cttggcgatc cactgacgcg ctacgagggg caaatcaagg
     3601 atgtgctacc gacgccggaa aaggtaaacg acgctatttt ctattacgcc cgttttgaag
     3661 tacccaaccc caatggtttg ctgcggctgg atatgaccgc gcaagtgcat attcagctca
     3721 ccgatgtgaa aaatgtgttg acgatccctc tgtcggcgtt aggcgatccg gttggcgata
     3781 atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg
     3841 gcgcacgtaa cgataccgat gttgagattg tcaaagggct ggaagcgggc gatgaagtgg
     3901 tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat
     3961 tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca tcagcctcaa
     4021 tatttatgcg ggtgagatgg tcgcgattgt tggcgcttcg ggttccggta aatcgaccct
     4081 gatgaatatt ctcggctgcc tggataaggc caccagcggc acctatcgcg tagccggtca
     4141 ggatattgcc acgctggacg ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt
     4201 tattttccag cgttaccatt tgcttccgca tttaaccgtc gagcagaacg ttgaagtacc
     4261 cgccgtctat gccggtcttg agcggaaaca gcgactgctt cgtgcccagg agttgctgca
     4321 acggctgggg ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca
     4381 acagcgcgtc agcatcgcgc gggcattgat gaacggcggt caggtaattc ttgccgatga
     4441 accgaccggc gcactggaca gccattctgg cgaagaggtg atggcgatcc tgcatcagct
     4501 gcgcgatcgc gggcatacgg tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc
     4561 cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa
     4621 agtgaatgtt gctggcggga cggagcctgt tgtcaataca gtgtctggct ggcggcagtt
     4681 tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat
     4741 gcgtacttta ctgaccatgc tggggattat tatcggtatt gcgtcggtgg tttccattgt
     4801 cgtggtgggt gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa
     4861 tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcagcaggc
     4921 gctgaagtac gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct cagccacacc
     4981 tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa
     5041 tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt
     5101 taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg
     5161 ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc
     5221 cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca gtaaagtgct
     5281 gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggcttaa
     5341 ctccattact gtcagggtga aagaaggatt tgacagcgcc gaggcggaac agcaactcac
     5401 gcgtttactt tcactgcgcc acggtaagaa ggatttcttt acctggaaca tggacggcgt
     5461 cttgaaaact gttgaaaaga ccacacgtac tttacaactg tttctgacgc tggtggcggt
     5521 gatttcgctg gtggtgggcg gtattggtgt aatgaatatt atgctggtgt cagtgaccga
     5581 gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca
     5641 acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataacact
     5701 gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc
     5761 accgttggcg ctgctgctgg cgtttctctg ctcgacggcc accgggattt tatttggctg
     5821 gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac gggagtaatt
     5881 tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga
     5941 gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat
     6001 tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt
     6061 aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc
     6121 agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc
     6181 gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct
     6241 gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagca
     6301 tttgacaggg gccagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac
     6361 aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat catgtggagt
     6421 agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc
     6481 gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat caactggcgg
     6541 aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata ttagtcaatg
     6601 atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt tcttatgatg
     6661 tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc atttgcggag
     6721 tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac gcgagggaga
     6781 atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa aaattggggg
     6841 aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc gccagagcgc
     6901 gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg ctcagtaacc
     6961 catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac
     7021 tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag gagcgcgaca
     7081 cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct
     7141 ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc tatcttcagc gaacaggagt
     7201 cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg gtgaacttta
     7261 tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc agccagccaa
     7321 acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg aacctgaacc
     7381 agcttgcgcg tgtgggcgga atcgacccac taattggtcg tgagaaagag ctggagcgtg
     7441 ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctggtgggg gaatctggtg
     7501 tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat tgttcagggt gatgtgccgg
     7561 aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta gcgggcacaa
     7621 aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg gagcaggaca
     7681 ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt gcagcgtctg
     7741 gtggtcaggt cgatgcggct aacctaatca aaccgttgct ctccagcggt aaaattcgcg
     7801 taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac cgtgctctgg
     7861 cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact gttcaaatca
     7921 tcaatggcct gaaaccgaag tatgaagcgc accacgacgt gcgttatacc gcaaaagcgg
     7981 tgcgtgcggc ggtagagctg gcggtgaaat acattaacga tcgtcatctg ccggataaag
     8041 ccattgatgt tatcgacgaa gcaggcgctc gcgcacgtct gatgccggta agcaaacgca
     8101 agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgca cgcattccag
     8161 agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa actcagcgat cgcctgaaaa
     8221 tgctggtctt cggccaggat aaagccattg aggcgctgac tgaagccatt aagatggcgc
     8281 gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt cctgtttgcc ggtcctaccg
     8341 gggtcgggaa aacagaggtg acggtacagc tttcgaaagc gttgggcatt gagcttctgc
     8401 gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtctgatt ggtgcgcctc
     8461 cgggatacgt tggttttgat cagggcggtt tgctgaccga tgcggtaatc aagcatccac
     8521 atgcggtgct gctgctggac gaaatcgaga aagcgcatcc ggacgtgttc aatattctgt
     8581 tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg gacttccgta
     8641 acgtggtgct ggtgatgacc actaacgccg gggtacggga aactgagcgc aaatccattg
     8701 gtcttatcca ccaggataac agcaccgatg cgatggaaga gatcaagaag atctttacac
     8761 cggaattccg taaccgtctc gacaacatta tctggttcga tcatctgtca actgacgtga
     8821 tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat cagaaaggtg
     8881 tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt tacgaccggg
     8941 caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa ccgctcgcca
     9001 acgaactgct gtttggttcg ctggtggacg gcggtcaggt gacggttgcg ctggataaag
     9061 agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg gaagcagcgc
     9121 attaatctga ttgtcaggta ggttggtgaa gtccgtaatc tcgtcagggg ttacggactt
     9181 tttatttatg gggtggaacg gtgaaaaccc tatttttgga ggtgaaggta agttgttgat
     9241 aattagtgct gctggaaggt aaggataaaa aagggtgctg caggagaatg ggatggtttt
     9301 gctttattaa caacgggcta aacgtgtagt atttgagttc actgccgtac aggcagctta
     9361 gaaatcgacg gggtgcggta aaacctttgc gaacgcgttc actgccgtac aggcagctta
     9421 gaaattcaca ggtaacatac tccacccgcc caccatgttc actgccgtac agacagataa
     9481 aatgcgaaaa aaaagctcgc actttcgtac gagctcttct ttaaatatgg cggtgagggg
     9541 gggattcgaa cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca
     9601 ctcggacacc tcaccaaatt gttttgctac caaacctcat gggtggcaac ggggcgctac
     9661 tatagggagt tggagtaaaa cggtcaagaa gaattttaat gataattatt gtttgctcat
     9721 actgtaaaca acttgtgcag tatatctaca tcgagacaag ttatggactt atacttccaa
     9781 agtacttcat acatatcaca aaataaaaag gccggttaaa ccgacctttt actcgttctt
     9841 tctcttcgcc catcaggcgg taaaacaatc agcgactacg gaagacaatg cggcctttgc
     9901 tcaggtcgta cggggtcagt tcaacagtca ctttgtcgcc cgtcaggatg cggatgtagt
     9961 ttttgcgcat tttaccggag atgtgtgcag taaccacgtg accgttttct aactctacgc
    10021 ggaacatggt attaggcaac gtttcaagaa cggtaccttg catttcaata ttgtcttctt
    10081 tggccatcta atcctctggg gtatcactac cgtaatttga accggcaaga taatgccgaa
    10141 gttctgtaaa taagtaaaga tttgcgcgct aaatcgcaac aaacaggttc ggcacattac
    10201 tccgaaaaca cacggctaag ccgcaccaaa agcgcaacgt ataagggagc ggtgagataa
    10261 acgatgggcg ttacctgacg cgaaaaattc cttatcggca gcggggtaat gagcgtaacc
    10321 aactctgcga ccgcaattat aacactctgg ggagaaatgt gccgaaaaca ttcattcttg
    10381 tggtgaaaac aagcaccgtg gtacccagaa attattcggc aatcgtccga ggcgcatttg
    10441 attgagataa ttaaggtaat cccggcgggg aatttcgcag gcaccaagcg atgctgtgtg
    10501 atcgttaagg acctggcagt cgataagctt accgccatga ccgataaatt cctcacagaa
    10561 taccagaagc gccgttttag acgcattttc catccggctg aacatggact cgccacaaaa
    10621 tagcgttccc tgggccacgc cgtacatacc gccgacaagc tcatcttcac gccagacttc
    10681 aatggagtgg gcatgaccga gttcgtgaag gcgatggtag gcttcgacca cgccacgcgt
    10741 gatccaggtt ccttcttcgc gatcgctggc acagccttca atgacctgac caaaagcgta
    10801 attcatcgtg acacgatagg gcgagcgttt atgaaatcgc ttcatactac ggctgatatg
    10861 cagtgattct ggccatagca ccgcgcgggg atcgggcgac caccagagga tggggtcgcc
    10921 tggagaaaac cacggaaaaa taccacgctg gtaagccatt aacaggcgcg cagggctaag
    10981 atcgccccca agtgccagca ggccgttagg ctcacgtaat gcgccttccg gggaagggaa
    11041 ggctattgaa tggcgagaaa gctgaaccaa gcgcatgacc gcaaaactcc acgcaagtcg
    11101 gatcgttcaa taatagctta caaaccctgc ttgaactggt aataacgccc ctgtctggca
    11161 agcagttctg cgtgagtacc ttgctcaata atttgcccgt tgtccatcac tattatttgt
    11221 tggaaacgag agagtccgcg aagtcgatgg gtgaccatta acaccgtttt ctcacgcatc
    11281 atttttgcaa gcaattcaag gatctggctt tcggttgtgg catctaagcc ttcggtaggt
    11341 tcatccagca acaccagtgg cgcatcatgt aacagcgtac gggcgatagc cagacggcgc
    11401 agttcaccac cggagagctg gcgtccgcct tcacctaacc aactgttgag acctgcatcc
    11461 tcgagcagct tctccagacc aacgcgacgc aagatctccg acagagcctc atcactactg
    11521 ccaggcgagg cgagtaacag attatcacgc agtgtggcgc taaacagatg cactcgctga
    11581 ggaacaacgc tgatggtctg tcgtagagcc gcttcattca ggctggctat ggggctatcg
    11641 ttaagcaaaa tctcgccctg ttgcgggtcc catgcgcggg tcagcagttg taacagtgtt
    11701 gatttgccgc atccggttcg cccgagaatc gctatatgtt ccccggcgtt tacctgaaga
    11761 gaaatccctt taagtgcctg ttgggattgc tccggataag tgaactgaac atcccgtaat
    11821 gtcagcgaaa cgcgatcggc aacacgagtt tgggtatcag ggaaggtgac ctccggtttt
    11881 tgatccgtta agtcagagat acgtacggca gaggcaatga cttgccccag atgctgaaat
    11941 gcacccgtta ctggtgccag tgcttcaaac gcggctaacg cacaaaagac aaacagggca
    12001 attaatgcgc cgggttgagc attgccgcca acgccgccag acgccatcca cagcatcagg
    12061 atcaccgcta acgcgccaat gagcagcatt atcgcttgcg acaatgcggt cagttcagat
    12121 tgacggcgtt gcgcttccag ccattgaatt tctgtattct ctagttgcgt gcgataacga
    12181 tcgctggcac caaaaatggt cagctcagct tgcccttgca gccaggccgt cagttgttgg
    12241 cgatactgtc cgcgaagatg agtcagattt tgcccggtgc tttttcccgc acgataaaac
    12301 agcggtggca tcaggaaaag cgtcagtaac ataacgctgc ccagcgtaaa ggcgagggtg
    12361 aaatcaagga aacttaaccc gattgtcacc accataatca ccacaaaagc gcccaccagc
    12421 ggcgagataa cgcgcaggta aagatgatcg agcgtatcaa catccgccac cacgcggttg
    12481 agcaattcgc cctgacgata gcgcgccagt ccggcagggg agaggggcaa caatttgctg
    12541 aaggtgtaaa tgcgcagatg ctgcaacacg cggaaagtcg cgtcgtgact taccagacgt
    12601 tcaaaataac gcccggcagt acgggtgatt gcggcaccac gcacgcccgc agcgggtagc
    12661 atatagttga agctgtacag tccggcaacc cccgcaaccg ctgaggccga gaggaaccag
    12721 ccggaaagtg tcaacagacc gatactggcg agcagcgtca caattgccag cacaatacca
    12781 agacttaaca tccatttatg acgtttatac agtgccagat agggtagcaa agcgcgcatt
    12841 taaatctcct cctgacgatg ggccagtaat gtggcgaatg ggccaccagc cacacttaat
    12901 tccgcgtaac gtccttgctc aataatccgg ccatcctgca taacccaaat gacatcccag
    12961 tcagcaagat cttctaactg gtgggtgacc attaacgttg tctggcgcag agaggcggca
    13021 ttcagcgcct ccattacgcg ctgttcactg tgagcatcaa ggctggcagc gggttcatcc
    13081 aacagtaata gcgaacaggg atttagtaac gcacgggcca ccgccacgcg ctgcgcctgc
    13141 cccacggaaa ggcgggcagc ctggtcgcca acaggtgtat caacgccttg tggcaggagc
    13201 ggtagaaact cgctgaccca ggcgttatcc agcgctgctt gtaattcttg ttcgctggca
    13261 tcaggtcgcg ccagtagtac gttatcccgc aattttgctg ccggtaattg tgggttttgc
    13321 ccaacccagg agagatgttt acgccatgat tctggtgata aatcacgtaa ttctatcccg
    13381 ttgattcgta atgatccctg atatgagaga aaaccagaaa gcgcgttcag cagtgagctt
    13441 ttacctgaac cgctgcggcc aaccaacact gcacgttggc ctgctggcaa agtaaagttc
    13501 agcggtccgg ccagcgtttt accttccggc gacgtgataa acagatcctc ggcctcaatg
    13561 gtcagcggat cggtcaatgc taattccgcc tcaccgcgct gcggatgggc gagcggggtt
    13621 tccataaacg ttttcagact gtcagctgca ccaacagcct gggctttagc atgataaaac
    13681 gtaccgagat cgcgtaatgg ctggaaaaac tctggcgcaa ggatcagggc cagaaaacct
    13741 gcagccagcg tcaccccagt atcgtagtga ccaaaatcca gctcgccgag ataggaaaaa
    13801 ccaaagtaga ccgccaccag agcaattgac agcgaggtaa aaaattcgag aatgccggag
    13861 gataaaaacg ccagtcgtag cacttccatt gtccgttggc ggaaatcttc cgaagcagaa
    13921 cgaatacttt caatttcagc ttcaccacga ccaaaaatac gcaatgtttc catgccgcgc
    13981 aggcgatcga ggaaatgccc acttaagcga gcaagagcga gaaagttacg ccggttagca
    14041 tcggcagccc ccattccaac cagcgccata aacaacggaa ttagcggtgc tgtgcccagc
    14101 agaatgagcg ccgcagccca gttagagggg aagatcgcca ccacaatcag caacggcacc
    14161 gacactgcca gcgccatttg cggcagatag cgtgcatagt aatcatgcat atcgtcaatt
    14221 tgctcgagta ccagcgtcgc ccagctcccc gcaggtttac cctgaatcca cgctggccct
    14281 gcttgttgca gacggtcgag aacctgacgg cggatggcaa agcggatatg ctgcccggcg
    14341 tgataaccca cccgttcgcg taaccagacc acccatgcgc gcaatacaaa ggtcagaacc
    14401 agtaacgtaa agggaagcag cagggcttca cggggaatat tctccataat catatgttgc
    14461 agaattcgcg ccatgaacca ggcctgggca atgatcaata tgccgcttac aaagcccagc
    14521 agacgagaaa tattcagcca acgttgggag atgacgcttt gctgttttaa ccagcgggtt
    14581 aactcttttt gacgagattt attcattgca cgcttagcag gtgagttatc agaattattt
    14641 gcagagcaat gttacaacgg ggaaaaaata aaggcgaccc atagtcgcaa ggtgtcgcct
    14701 tctttacttt tgttactgat ttgtaaaatt attttgcgtc agctaaacca tcgaggtagc
    14761 gttccgcatc aagtgctgcc atgcagccag taccggccga agtaatggcc tggcgataaa
    14821 tgtgatccat cacgtcgcct gcggcaaaga cgccaggaat gctggtctgg gtggcattac
    14881 catgaatacc cgactgtact ttgatgtagc cgttttccag ttccagctgc ccttcgaaaa
    14941 tcgcagtatt cgggctgtga ccgatagcaa caaacagacc ggcaacgtcg agtgactcga
    15001 tgttatcgct gttttgcgta tcgcgcagac gaacgccagt gacacccatt tgatcgccgg
    15061 tcacttcttc cagcgtacgg ttggtgtgca gaatgatgtt gccgttctcc actttatcca
    15121 tcagacgctt aataaggatt ttttccgcgc ggaaaccgtc acggcggtga atcagatgca
    15181 cttccgaagc gatgttagac agatacagcg cctcttcaac cgcggtattg ccgccgccga
    15241 tgaccgcaac tttctggttg cgatagaaga aaccgtcgca ggttgcacaa gcagaaaccc
    15301 cacggccttt aaaggcttct tcagagggca ggccgagata gcgtgcagaa gctccggtgg
    15361 caataatcag cgcgtcgcaa gtgtattcgc cgttatcgcc attcagacgg aacggacggt
    15421 tttgcagatc caccttgttg atatgatcaa aaatgatctc agtttcaaac ttggtggcat
    15481 gttcgtgcat gcgcgccatt aataacggac cggtcagatc gtttggatca ccaggccagt
    15541 tttccacttc cgtggtggtg gtcagttggc cgcctttttc catgccggta atcagcacag
    15601 gttgcaggtt ggcgcgcgcc gcgtagacag cagcggtgta tcccgccggg cctgaaccca
    15661 ggataagcag tttactgtgt ttggtcgtgc ccatgagatc cccatagttg ttggcagaca
    15721 atgggcagga ttgtagggaa tttacagacg taaaaaaaga gtatgacgat tttgttaaca
    15781 atttgtgcaa tcggcagcat cgataagcag gtcaaattct cccgtcatta tcacctctgc
    15841 tacttaaatt tcccgcttta taagccgatt aaatgatgaa taaacgcccc tgttaatgaa
    15901 tatctggcat gttgtactaa aaatcgatgt tttgctttga caatcccctg gtgttttgcg
    15961 aaaacattcg aggaagaaaa aaacagtatt cttatatgcg cataaccatg tatgtaaata
    16021 ccatgtttac cgtgctagtg aaatctacgt atggcgtgga cagacgccat tcgtgatgtc
    16081 gatagctgcc acaaggcaac ggtcttctca ccgtagaccc aggcattgcg cgccgtgaat
    16141 cttcatgatt tcggtctatc gtgacgggta gcgactctga acagtgatgt ttcagggtca
    16201 gacaggagta gggaaggaat acagagagac aataataatg gtagatagca agaagcgccc
    16261 tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat gagttgcaaa aggatgggcg
    16321 tatttctaac gtcgagcttt ctaaacgtgt gggactttcc ccaacgccgt gccttgagcg
    16381 tgtgcgtcgg ctggaaagac aagggtttat tcagggctat acggcgctgc ttaaccccca
    16441 ttatctggat gcatcacttc tggtattcgt tgagattact ctgaatcgtg gcgcaccgga
    16501 tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa gaaattcagg agtgtcattt
    16561 agtatctggt gatttcgact acctgttgaa aacacgcgtg ccggatatgt cagcttaccg
    16621 taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc aatgacacac ggacatacgt
    16681 tgttatggaa gaagtcaagc agagtaatcg tctggttatt aagacgcgct aacacggaac
    16741 aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat ccatacagca acagtactgg
    16801 ggtaacctgg tactgttgtc cgttttagca tcgggcagga aaagcctgta acctggagag
    16861 cctttcttga gccaggaata cactgaagac aaagaagtca cattgacaaa gttaagtagc
    16921 ggccgccgcc ttctggaggc attgctgatc cttattgtcc tgtttgccgt ctggttgatg
    16981 gctgccttac taagctttaa cccttcggac cccagctggt cgcaaacggc ctggcatgaa
    17041 cctatccata atttaggtgg gatgcccggt gcgtggttgg cagatacgct gttctttatt
    17101 tttggcgtga tggcttacac cattcccgtc attattgtcg gcggttgttg gtttgcctgg
    17161 cgtcatcagt ccagcgacga atacattgat tattttgccg tttcgctacg catcattggc
    17221 gttttggcgc tcatccttac ctcctgtggt ctggcggcaa tcaacgctga cgatatctgg
    17281 tattttgcct ccggtggcgt cattggcagc ttactaagca ctacgctaca accactgcta
    17341 cacagtagcg ggggaactat tgcgctgctc tgcgtttggg cagcgggcct gacgttgttc
    17401 accggttggt catgggtgac cattgctgaa aaactcggcg gctggatttt aaacattctc
    17461 accttcgcca gtaatcgtac ccgtcgcgat gatacctggg tcgatgaaga tgagtatgaa
    17521 gacgacgaag agtatgaaga tgaaaatcac ggcaaacagc atgaatcacg ccgtgcccgt
    17581 attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg
    17641 gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga tggatgatga cgaagagatt
    17701 acctacactg cacgcggtgt ggctgccgac ccggacgacg tcctattttc gggcaatcgt
    17761 gcaacgcagc cagaatatga cgaatacgat ccattattaa acagtgcgcc aattaccgaa
    17821 cctgtcgctg tagcagctgc tgctaccacg gcgacacaaa gctgggctgc gccggttgaa
    17881 cctgtgactc agacgccgcc tgttgcctct gttgatgttc caccttcgca acctacagta
    17941 gcctggcagc ctgtaccggg tccacaaacg ggagagccgg ttattgctcc tgcaccggaa
    18001 ggttacccac agcagtcaca atatgcgcag cctgcagtgc aatataatga gccgctgcaa
    18061 caaccagtac agccgcagca gccgtattat gcacctgcag ctgaacaacc tgcgcaacag
    18121 ccgtattatg cccctgcgcc agaacaaccg gtggcaggta acgcctggca agccgaagag
    18181 cagcaatcca cttttgctcc acagtctaca taccagactg agcaaactta tcagcagcca
    18241 gccgctcagg agccgttgta ccaacagccg caacccgttg aacagcagcc tgttgtggag
    18301 cctgaacccg ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta ttttgaagaa
    18361 gtggaagaga agcgagcccg tgaacgtgaa caacttgcgg cctggtatca accgattcca
    18421 gaaccggtta aagaaccaga accgatcaaa tcttcgctga aagcaccttc tgttgcagca
    18481 gtacctccag tagaagccgc tgccgctgtt tctccgctgg catctggcgt gaaaaaagcg
    18541 acactggcga cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc
    18601 ggtggaccgc gtccgcaggt caaagagggg attggcccgc agttgccacg accgaaacgt
    18661 atccgcgtgc caactcgtcg tgaattggcg tcttacggta ttaagctgcc ctcacagcgt
    18721 gcggcggaag aaaaagcccg tgaagcccag cgcaatcagt atgattctgg cgatcagtat
    18781 aacgatgatg aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt cgcccagaca
    18841 cagcagcaac gctatggcga acagtatcaa catgatgtgc ccgtaaacgc agaagatgca
    18901 gatgctgcgg cagaggctga actggctcgt cagtttgcgc aaactcaaca acaacgttat
    18961 tccggcgaac aaccggctgg ggcgaatccg ttctcgctgg atgattttga attttcgcca
    19021 atgaaagcgt tgctggatga tggtccacac gaaccgctgt ttacgccaat tgttgaacct
    19081 gtacagcagc cgcaacaacc ggttgcacca cagcagcagt atcagcagcc acaacagccg
    19141 gttgcgccac agcagcagta tcagcagcca caacagcagg ttgcgccgca gccacaatat
    19201 cagcagccgc aacaaccggt tgcgccacag cagcaatatc agcagccgca acaaccagtt
    19261 gcgccgcagc cgcagtatca acagccgcaa caaccggttg caccgcagcc gcagtatcag
    19321 cagccacaac agccagttgc gccacagccg caggataccc tgcttcatcc gctgttgatg
    19381 cgtaatggcg acagccgtcc gttgcacaaa ccgacgacgc cactgccttc tctggatttg
    19441 ctgacaccgc cgccgagcga agtggagccg gtagatacct ttgcgcttga acaaatggcg
    19501 cgtctggtgg aagcgcgtct ggctgatttc cgtattaaag ccgatgtcgt taattactct
    19561 ccggggccag ttatcactcg ctttgaactt aacctggctc ctggcgtaaa agcggcgcgc
    19621 atttctaact tgtcacggga ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt
    19681 gaagttattc ctggcaaacc ctatgtaggt ctggagttac caaataaaaa acgacaaacc
    19741 gtttatctgc gcgaagtttt ggataacgcc aaattccgcg ataatccgtc gccattaacc
    19801 gtggtgctgg gtaaagatat cgccggtgag ccggtggttg ccgatctggc gaaaatgccg
    19861 cacttgttgg ttgcggggac taccggttcc ggtaaatctg tcggtgtgaa cgcgatgatc
    19921 ctgagcatgc tttataaagc acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa
    19981 atgctggagc tttcggttta tgaaggcatt ccgcacctgt taacggaagt cgttactgat
    20041 atgaaagatg ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg tcggtataaa
    20101 ctgatgtctg cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc
    20161 gatcgcatga tgcgtccgat tccagacccg tactggaagc cgggtgacag tatggatgcc
    20221 cagcatccgg tgctgaaaaa agaaccatac attgtcgtgt tggttgacga atttgccgac
    20281 ctgatgatga cggtaggtaa aaaagtggaa gagctgatag ctcgtctggc gcaaaaagcc
    20341 cgtgccgcgg gtatccacct cgtactggca actcagcgtc catcggttga tgttattact
    20401 ggtctgatta aagcgaatat tccgacccgt atcgccttta ccgtatccag taagattgac
    20461 tcacgtacca ttcttgatca ggctggcgcg gaatcactgc tgggtatggg ggatatgctc
    20521 tactctgggc cgaactccac gttgccggta cgtgtccatg gtgcttttgt tcgcgatcag
    20581 gaagttcatg ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta tgttgatggc
    20641 atcacctccg acagcgaaag cgaaggtggt gcgggtggtt tcgatggcgc tgaagaactg
    20701 gatccgttgt tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc gtcaatttct
    20761 ggcgtacagc gtcagttccg cattggttat aaccgtgcag cgcgtattat cgaacagatg
    20821 gaagcgcagg ggattgtcag cgaacagggg cacaacggta atcgtgaagt gctggcccca
    20881 ccgccgtttg actaattaat gcattgccgg ataaggcgcg gtagcgtcgc atccggcact
    20941 ctatcaactg aaaattcagt attttcttct ttcctcaagc tgattattag cctggaatag
    21001 agagtagagg gaactcccga tcgggagtga cgtaatttga ggaataatga tgaaaaaaat
    21061 tgctatcacc tgtgcattac tctcaagctt agtagcaagc agcgtttggg ctgatgccgc
    21121 aagcgatctg aaaagccgcc tggataaagt cagcagcttc cacgccagct tcacacaaaa
    21181 agtgactgac ggtagcggcg cggcggtgca ggaaggtcag ggcgatctgt gggtgaaacg
    21241 tccaaactta ttcaactggc atatgacaca acctgatgaa agcattctgg tttctgacgg
    21301 taaaacactg tggttctata acccgttcgt tgagcaagct acggcaacct ggctgaaaga
    21361 tgccaccggt aatacgccgt ttatgctgat tgcccgcaac cagtccagcg actggcagca
    21421 gtacaatatc aaacagaatg gcgatgactt tgtcctgacg ccgaaagcca gcaatggcaa
    21481 tctgaagcag ttcaccatta acgtgggacg tgatggcaca atccatcagt ttagcgcggt
    21541 ggagcaggac gatcagcgca gcagttatca gctgaaatcc cagcaaaatg gggctgtgga
    21601 tgcagcgaaa tttaccttca ccccgccgca aggcgtcacg gtagatgatc aacgtaagta
    21661 gaggcacctg agtgagcaat ctgtcgctcg atttttcgga taatactttt caacctctgg
    21721 ccgcgcgtat gcggccagaa aatttagcac agtatatcgg tcagcaacat ttgctggctg
    21781 cggggaagcc gttgccgcgc gctatcgaag ccgggcattt gcattctatg atcctctggg
    21841 ggccaccggg taccggcaaa acaaccctcg ctgaagtgat tgcccgctat gcgaacgctg
    21901 atgtggaacg tatttctgcc gtcacctctg gcgtgaaaga gattcgcgag gcgatcgagc
    21961 gcgcccggca aaaccgcaat gcaggtcgcc gcactattct ttttgttgac gaagttcacc
    22021 gtttcaacaa aagccagcag gatgcatttc tgccacatat tgaagacggc accatcactt
    22081 ttattggcgc aaccactgaa aacccgtcgt ttgagcttaa ttcggcactg ctttcccgtg
    22141 cccgtgttta tctgttgaaa tccctgagta cagaggatat tgagcaagta ctaactcagg
    22201 cgatggaaga caaaacccgt ggctatggtg gtcaggatat tgttctgcca gatgatacac
    22261 gacgtgccat tgcagaactg gtgaatggcg acgcgcgccg ggcgttaaat acgctggaaa
    22321 tgatggcgga tatggccgaa gtctatgata gcggtaagcg ggtcctgaag cctgaattac
    22381 tgaccgaaat cgccggtgaa cgtagcgccc gctttgataa caaaggcgat cgcttttacg
    22441 atctaatttc cgcactgcat aagtcggtac gtggtagcgc acccgatgcg gcgctgtact
    22501 ggtatgcgcg aattattacc gctggtggcg atccgttata tgtcgcgcgt cgctgtctgg
    22561 cgattgcgtc tgaagacgtc ggtaatgccg atccacgggc gatgcaggtg gcaattgcgg
    22621 cctgggattg ctttactcgc gttggcccgg cggaaggtga acgcgccatt gctcaggcga
    22681 ttgtttacct ggcctgcgcg ccaaaaagca acgctgtcta cactgcgttt aaagccgcgc
    22741 tggccgatgc tcgcgaacgc ccggattatg acgtgccggt tcatttgcgt aatgcgccga
    22801 cgaaattaat gaaggaaatg ggctacgggc aggaatatcg ttatgctcat gatgaagcaa
    22861 acgcttatgc tgccggtgag gtttacttcc cgccggaaat agcacaaaca cgctattatt
    22921 tcccgacaaa caggggcctt gaaggcaaga ttggtgaaaa gctcgcctgg ctggctgaac
    22981 aggatcaaaa tagccccata aaacgctacc gttaatgtta tcgttgcggt aatgttgtta
    23041 ctgtatccct gtggtcgcag gctgtggcca catctcccat ttaattcgat aagcacagga
    23101 taagcatgct cgatcccaat ctgctgcgta atgagccaga cgcagtcgct gaaaaactgg
    23161 cacgccgggg ctttaagctg gatgtagata agctgggcgc tcttgaagag cgtcgtaaag
    23221 tattgcaggt caaaacggaa aacctgcaag cggagcgtaa ctcccgatcg aaatccattg
    23281 gccaggcgaa agcgcgcggg gaagatatcg agcctttacg tctggaagtg aacaaactgg
    23341 gcgaagagct ggatgcagca aaagccgagc tggatgcttt acaggctgaa attcgcgata
    23401 tcgcgctgac catccctaac ctgcctgcag atgaagtgcc ggtaggtaaa gacgaaaatg
    23461 acaacgttga agtcagccgc tggggtaccc cgcgtgagtt tgactttgaa gttcgtgacc
    23521 acgtgacgct gggtgaaatg cactctggcc tcgactttgc agctgcagtt aagctgactg
    23581 gttcccgctt tgtggtaatg aaagggcaga ttgctcgcat gcaccgcgca ctgtcgcagt
    23641 ttatgctgga tctgcatacc gaacagcatg gctacagtga gaactatgtt ccgtacctgg
    23701 ttaaccagga cacgctgtac ggtacgggtc agctgccgaa atttgctggc gatctgttcc
    23761 atactcgtcc gctggaagaa gaagcagaca ccagtaacta tgcgctgatc ccaacggcag
    23821 aagttccgct gactaacctg gtgcgcggtg aaatcatcga tgaagatgat ctgccaatta
    23881 agatgaccgc ccacacccca tgcttccgtt ctgaagccgg ttcatatggt cgtgacaccc
    23941 gtggtctgat ccgtatgcac cagttcgaca aagttgaaat ggtgcagatc gtgcgcccag
    24001 aagactcaat ggcggcgctg gaagagatga ctggtcatgc agaaaaagtc ctgcagttgc
    24061 tgggcctgcc gtaccgtaaa atcatccttt gcactggcga catgggcttt ggcgcttgca
    24121 aaacttacga cctggaagta tggatcccgg cacagaacac ctaccgtgag atctcttcct
    24181 gctccaacgt ttgggatttc caggcacgtc gtatgcaggc acgttgccgc agcaagtcgg
    24241 acaagaaaac ccgtctggtt cataccctga acggttctgg tctggctgtt ggtcgtacgc
    24301 tggttgcagt aatggaaaac tatcagcagg ctgatggtcg tattgaagta ccagaagttc
    24361 tgcgtccgta tatgaacgga ctggaatata ttggctaata cccaattttt ctgaatctaa
    24421 aaagcgcctt tagggcgctt tttttgtctc cctttgatac cgaacaataa ttactcctca
    24481 cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt tcaaattatt
    24541 ctatgtatac aagcctatat agcgagctgc tatagaaata attacacaat acggtttgtt
    24601 actggaatca atcgtgatca agcttgagtg agccattatg aaaacgaaaa tccctgatgc
    24661 ggtattggct gctgaggaga gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct
    24721 ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg ctgtcgatag
    24781 cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag ttaactgtgg
    24841 tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg tcgaaacgac
    24901 aataccggcg atgacaatta cgacggccta cactaggttc gcgcctgcct gcgtgggcgt
    24961 tccatgcgtc accgtgtcta caatccggac cgcctgaaat atccgatgaa acgagtcggg
    25021 gcgcgcggtg aaggcaaatt cgagcgcatt agctgggaag aagcctacga catcatcgcg
    25081 accaatatgc agcgcctgat caaagagtac ggcaacgagt ctatctatct gaactatggc
    25141 accggtacgc tgggcggcac catgacccgc tcctggccgc cgggaaatac cctggtcgcg
    25201 cgcctgatga actgctgcgg cggttatctt aaccattacg gtgactactc ttccgcgcaa
    25261 attgcggaag gtttgaacca tacctacggc ggctgggcag atggtaacag cccgtcggat
    25321 atcgaaaaca gtaaattggt agtgctgttt ggtaataacc caggcgaaac gcgaatgagt
    25381 ggcggtgggg tgacttacta tcttgaacag gcacgccaga aatctaatgc tcgcatgatc
    25441 atcatcgatc cgcgctatac cgacaccggt gccgggcgcg aagatgagtg gatccctatt
    25501 cgtccaggaa cagatgccgc actggttaac ggtctggcgt acgtcatgat cactaaaaac
    25561 ctggtggatc aggcattcct cgataaatat tgcgttggct atgacgagaa aaccctgccc
    25621 gccagtgcgc cgaaaaatgg tcattataaa gcttatattc tgggtgaagg gccagatggc
    25681 gtggctaaaa caccggaatg ggcctcgcaa atcaccggtg ttccggcaga caaaatcatc
    25741 aaactggctc gtgaaatcgg cagtaccaaa ccggcgttta tcagccaggg atggggcccg
    25801 cagcgtcacg ctaacggtga aatcgcaacc cgtgctatct cgatgctggc gattctgacc
    25861 ggtaacgttg gtattaatgg agggaacagc ggcgcgcgtg aaggttcata cagcttaccg
    25921 tttgtccgta tgccgacctt ggaaaacccg atccagacca gcatttcgat gtttatgtgg
    25981 accgatgcca ttgaacgtgg cccggaaatg acggcgctgc gtgatggtgt gcgcgggaaa
    26041 gataagctgg atgtgccgat caaaatgatc tggaactatg ccggtaactg cctgattaac
    26101 cagcattctg aaatcaaccg tacccatgaa atcctccagg atgataagaa gtgcgagctg
    26161 attgtggtta tcgactgcca catgacctca tcggcgaaat atgctgacat cctgctgcct
    26221 gactgcaccg cttccgaaca gatggacttt gcactggatg catcctgcgg gaatatgtct
    26281 tacgtgattt tcaacgatca ggtgattaaa ccgcgctttg aatgtaagac catctatgaa
    26341 atgaccagcg aactggcaaa acgtcttggc gttgagcaac agtttactga aggccgtacc
    26401 caggaagagt ggatgcggca tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca
    26461 acgtttgaag agttccgcaa gcaggggatc tttaaaaagc gcgacccaca agggcatcac
    26521 gttgcttata aagccttccg tgaagatccg caggcaaacc cactgactac gccatcgggc
    26581 aaaattgaga tttattcgca ggcgctggct gacattgccg ctacctggga attgccagaa
    26641 ggcgatgtga tcgatccact gccgatctac acgccgggct ttgaaagtta tcaggatccg
    26701 ctgaacaaac agtatccgct gcagcttaca ggtttccact ataaatctcg cgttcactca
    26761 acttacggca acgttgatgt gctgaaagcg gcttgccgtc aggaaatgtg gatcaacccg
    26821 cttgatgccc aaaaacgcgg tatccacaac ggcgataaag tcaggatctt taacgatcgt
    26881 ggtgaggttc atattgaggc gaaagtgacg ccacgaatga tgccgggcgt ggtcgcactg
    26941 ggtgaaggcg cctggtatga cccggatgca aaacgtgtcg ataagggcgg ttgtatcaac
    27001 gtactgacca ctcaacgtcc gtctcctctc gctaagggga atccgtcaca tacaaacctt
    27061 gttcaggttg aaaaggtgta aggagtaacc gatgacaacc cagtatggat tttttattga
    27121 ttccagccgt tgcaccggtt gcaaaacctg cgagctggct tgtaaagact acaaagattt
    27181 gacgccagaa gtcagtttcc gccgcattta tgaatatgct ggcggcgact ggcaggaaga
    27241 taacggtgtc tggcaccaga acgtgtttgc ctactatctg tcgatttcgt gcaaccactg
    27301 cgaagatccg gcttgtacta aagtctgccc gagcggtgcg atgcataaac gcgaagatgg
    27361 ttttgttgtg gtcgatgaag atgtatgcat tggctgccgt tactgtcata tggcttgccc
    27421 gtatggcgca ccgcaatata acgaaacgaa aggccatatg accaaatgcg atggttgtta
    27481 tgaccgtgtt gcggagggta aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct
    27541 ggatttcggc cctatcgacg agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc
    27601 gttgccgcga gctcacttta ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg
    27661 cccgaccggg gataccactg gctatctggc aaacccgaag gaggtgtgag atgggaagtg
    27721 gatggcatga atggccgctg atgatcttca cggtcttcgg gcaatgtgtg gcgggtggtt
    27781 ttatcgttct ggctttggcg ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg
    27841 ttatcgcctg catgtttggc ttatgggtac tgatgggcat tggctttatc gcctctatgc
    27901 tccatcttgg ttcaccgatg cgcgccttta actcgctcaa ccgggtaggg gcttcagcac
    27961 tcagtaacga aatcgccagc ggttcgatct tttttgctgt aggcggcatc ggctggttgc
    28021 tggcaatgct gaaaaagctg tcaccggcat tgcgtacgct atggctggtg gtgacgatgg
    28081 ttcttggcgt cgtctttgtc tggatgatgg tgcgtgtgta taacagcatt gataccgttc
    28141 cgacctggta cagcatctgg acgccgatgg gctgggcgat gcgtctgctg ccagccattt
    28201 ctgtactggc actggtggtg agtggcgtga tgtcagtgat gcagggcaca gagctggcaa
    28261 ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt
    28321 cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag
    28381 gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg gcaggggaat
    28441 taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct
    28501 aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc gttaacgtgt
    28561 cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac gtcgccagtc
    28621 cttcaatatc attacgccag tcacggtgca gctcgcaagc cacgccgaac catgtcatca
    28681 attgcgcacc agcttgcgac atgcgatccc acgccgaatg ccgggtaatt tcattaaagg
    28741 tccctgatgc atcagtcacg acaaagacat caaacccttc ttcaatggct gaaagcgccg
    28801 ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt ttacctgtcg
    28861 ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatattgccc gggcgagcaa
    28921 tgtaaggtgc atcggggaat tgtgctttca gttctagaac taatgggccg ttaggaccgg
    28981 tttcaaaact ggtggtaaga atagttggta agttgaaata cttagccagg tcacccagcg
    29041 ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa agtaaaccag
    29101 cctggtgatc aacaagcaaa acggcagcat catttttatc aagacgaaca tacggtttgg
    29161 tcatcctctt ctcctttcga gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat
    29221 tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt tttcgactga
    29281 acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat taatcatctc
    29341 ttcagcatta gcgttatgaa tgagtaatat aaatcgataa taccgccgct gcgatatgcg
    29401 cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga aatttgaact
    29461 tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt gctgtctggc
    29521 ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc
    29581 caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt taccggcaac
    29641 cttgtcggta cattgctgac agggtatgtc attaaacgca ttggctttaa ccgcagctat
    29701 tatctgtcct ccttcatttt tgctgctggc tgtgccggcc ttggcctgat gattggtttc
    29761 tggagctggt tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat ttgggtggtt
    29821 gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg
    29881 tatatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag caaagtctca
    29941 accgagctga tgagcgtatt gccgtgggtt acaggtttga cattggcagg gatcttaccg
    30001 ctgttgttta cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac gtcaattacg
    30061 gcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc
    30121 gttctgggat ctctatatgg cctgatgcct ctgtacctca atcacaaagg ggtgagcaat
    30181 gccagtattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg
    30241 attggacgtc tggcggataa gtttggtcga ctgctggttt tgcgtgttca ggtctttgtc
    30301 gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc gttattcatc
    30361 ctcggtgccg ctggctttac gctatatccg gtggcgatgg cctgggcttg tgagaaagtt
    30421 gaacatcatc aactggtggc gatgaaccaa gccttactgt tgagctatac cgtgggaagt
    30481 ctgcttggcc cgtcatttag cgctatgcta atgcagaatt tctccgataa tttattgttt
    30541 atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgcttcgcaa cgccggtcat
    30601 acgccgaaac ccgttgctca cgtgtaaatg aattcaagca aagtgtgaac ttactgtttc
    30661 acactctgct tttttgtttc ttctatctga cttgcttttt tccaaatttt attcgtttaa
    30721 aaataaaatg tgcagcaggt tataattttg catttcgcta tttccgcact tcttatttgc
    30781 cgcgcataat ccctcgtttt acagatgccc ctttaatttt ggcgaaggat ttgtctatgg
    30841 ctgggaatgt tcagg
//
LOCUS       DAJTRM010000042        30288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRM010000042 DAJTRM010000000
VERSION     DAJTRM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.042"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..807)
                     /gene="wcaD"
                     /locus_tag="OO322_002015"
     CDS             complement(<1..807)
                     /gene="wcaD"
                     /locus_tag="OO322_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5906905.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLG"
     gene            complement(782..1999)
                     /gene="wcaC"
                     /locus_tag="OO322_002016"
     CDS             complement(782..1999)
                     /gene="wcaC"
                     /locus_tag="OO322_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5906906.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1996..2484)
                     /gene="wcaB"
                     /locus_tag="OO322_002017"
     CDS             complement(1996..2484)
                     /gene="wcaB"
                     /locus_tag="OO322_002017"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5906907.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2487..3326)
                     /gene="wcaA"
                     /locus_tag="OO322_002018"
                     /pseudo
     CDS             complement(2487..3326)
                     /gene="wcaA"
                     /locus_tag="OO322_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3419..5581)
                     /gene="wzc"
                     /locus_tag="OO322_002019"
     CDS             complement(3419..5581)
                     /gene="wzc"
                     /locus_tag="OO322_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5906908.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5584..6027)
                     /gene="wzb"
                     /locus_tag="OO322_002020"
     CDS             complement(5584..6027)
                     /gene="wzb"
                     /locus_tag="OO322_002020"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5906909.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6033..7172)
                     /gene="wza"
                     /locus_tag="OO322_002021"
     CDS             complement(6033..7172)
                     /gene="wza"
                     /locus_tag="OO322_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5906910.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7481..7630
                     /locus_tag="OO322_002022"
     CDS             7481..7630
                     /locus_tag="OO322_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906911.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7831..9414
                     /locus_tag="OO322_002023"
     CDS             7831..9414
                     /locus_tag="OO322_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5906912.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9866..11719)
                     /gene="asmA"
                     /locus_tag="OO322_002024"
     CDS             complement(9866..11719)
                     /gene="asmA"
                     /locus_tag="OO322_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5906913.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNSQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11741..12322)
                     /gene="dcd"
                     /locus_tag="OO322_002025"
     CDS             complement(11741..12322)
                     /gene="dcd"
                     /locus_tag="OO322_002025"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5906914.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12414..13055)
                     /gene="udk"
                     /locus_tag="OO322_002026"
     CDS             complement(12414..13055)
                     /gene="udk"
                     /locus_tag="OO322_002026"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5906915.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13373..13615
                     /locus_tag="OO322_002027"
                     /pseudo
     CDS             13373..13615
                     /locus_tag="OO322_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13614..14555
                     /gene="mdtA"
                     /locus_tag="OO322_002028"
                     /pseudo
     CDS             13614..14555
                     /gene="mdtA"
                     /locus_tag="OO322_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14555..17677
                     /gene="mdtB"
                     /locus_tag="OO322_002029"
                     /pseudo
     CDS             14555..17677
                     /gene="mdtB"
                     /locus_tag="OO322_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17678..20743
                     /gene="mdtC"
                     /locus_tag="OO322_002030"
     CDS             17678..20743
                     /gene="mdtC"
                     /locus_tag="OO322_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5906916.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20744..22159
                     /locus_tag="OO322_002031"
     CDS             20744..22159
                     /locus_tag="OO322_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5906917.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22156..23559
                     /gene="baeS"
                     /locus_tag="OO322_002032"
     CDS             22156..23559
                     /gene="baeS"
                     /locus_tag="OO322_002032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5906918.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23556..24278
                     /gene="baeR"
                     /locus_tag="OO322_002033"
     CDS             23556..24278
                     /gene="baeR"
                     /locus_tag="OO322_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5906919.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24469..24801
                     /locus_tag="OO322_002034"
     CDS             24469..24801
                     /locus_tag="OO322_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5906920.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24948..26309
                     /gene="yegQ"
                     /locus_tag="OO322_002035"
     CDS             24948..26309
                     /gene="yegQ"
                     /locus_tag="OO322_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5906921.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26651..26968)
                     /locus_tag="OO322_002036"
     CDS             complement(26651..26968)
                     /locus_tag="OO322_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906922.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27374..28273
                     /gene="yegS"
                     /locus_tag="OO322_002037"
     CDS             27374..28273
                     /gene="yegS"
                     /locus_tag="OO322_002037"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5906923.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28355..29128)
                     /locus_tag="OO322_002038"
     CDS             complement(28355..29128)
                     /locus_tag="OO322_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5906924.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29234..30274)
                     /gene="gatD"
                     /locus_tag="OO322_002039"
     CDS             complement(29234..30274)
                     /gene="gatD"
                     /locus_tag="OO322_002039"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5906925.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7006 a         8045 c         7830 g         7407 t
ORIGIN      
        1 gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaacccgga atacagccag
       61 agaaatcaat gctaacggta attttttctt aatcgcctct ttattcagat attgaaatgc
      121 ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc cggataatat
      181 tatccctgcg agaatcaggg catctgtttt aggcgttttg ataccaaact gtttgatgct
      241 gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa gtgctgtcgt
      301 gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa tcagatattt
      361 cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca tctccaccgc
      421 cgccagcgcc accaccagtc cgaccaccag ataaaagaaa cgtaatatct tacgatggtt
      481 gtgtggcgaa attgttttaa aacgaatact ccacaccatg ccaataatga tcacaatata
      541 gacaaacagc atagttgaag tgacgtattt accggcatcc aacgactgac caaacaggta
      601 gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt taatgctgat
      661 gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga tggggaaact
      721 ttcgccaagc tgggcgattt tgacgttaac cagcaggtag atcagtggca gcagcaggta
      781 gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca ctgtaggcgg
      841 cgcgactgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc gcgatttccg
      901 gtttgcttaa ctgtaccagt tgcagcacct cttcttcact gacggtttta ccaccggatt
      961 tttgcagcac ttcccgcgcc gcatcgctat gggtggcaat caccggcacg ccaatcgata
     1021 gcgcctcaca caaaatcagc gggtagttat cgacacgaga actgaacacc agcgcatcca
     1081 tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga ttaaccacgt
     1141 tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg tcgcccagcg
     1201 ccatcatctc acgcaccagt tgctggttag ttttgccgtc gtaacgcagg tcatgcgcca
     1261 ccactgcgat tttcggcttg ccctgagttt cgcgcaccgg aggcagatct gccagaatcg
     1321 cttcggttgc catatcaatg ccattattga taatccggca acgccctggc ccgtacagac
     1381 tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc agcgccagca
     1441 tctcgcggaa taactggcgt ttgcccgcca ccagctggtg cgcgcgatca atcttcaccg
     1501 gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct tcgcaaccgt
     1561 cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag accagggtga
     1621 catccggttt atggtttttc actttttcgc aaaagcgcac cacgctgtta agattcaacc
     1681 agtagctgtg cagcacatga aaatgcagga ccaccggacc cggtgtgcga gtaatggtgc
     1741 gatataactc attgaaattg ccgaacagat cgcgattaaa cagacgaaac aacgcaatgt
     1801 tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc tgatggctga
     1861 cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc ccctgttgca
     1921 gcgcacgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc agtcgcacat
     1981 taaattgcag aatattcatt taatcacctt cactcgcgct ttttctccca ccaccagcgc
     2041 gttgtccggg acagagtcga gcaccacgct gcccgcaccc acggtgacgt tgttaccaag
     2101 cgtgatatca ccaagaataa tgacgttggc accgagttcg acgccgttgc caatgtgtgg
     2161 acatgccatg ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa tggtgaaatc
     2221 atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga tagtaaagcg
     2281 gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg tgataatgcg
     2341 atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt tgcgccacac
     2401 cgaacaaaaa tgagcgacgc gataggcaag taccatgcag cacgggcgta aactccagct
     2461 gttggcgcgc agatcttcca gcatctttag cgcccccgaa taccatcagc cagacgtttg
     2521 ccgttacgca ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt attgcggatc
     2581 tggtagaggg taaacagctg atatttttta ctggcgcggt cgaatttgtc tttgtgcttg
     2641 cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg catttcgccg
     2701 tgattgatat gcaggatctg cgtcgcctct tccactttcc acggttcgcc gtactccacc
     2761 accatccgca ggaatatgtc gtaatcctgt gctgctttca gttcggtatc gaacaaacac
     2821 tctttgaaac gccatgccca ggtaaagacc tgattgccaa tgatattacg tttatagaac
     2881 aggcggcgtg agtacggcga tttgggatac agtggtaagc tcgccggttg ggaataaact
     2941 tctccctagc aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag ttgctgttta
     3001 tgggcgagga agacgctcag gcggttgggc gtccattcat catcatcatc gatcccggtg
     3061 atgtattccc cttgcgccag cataatcgcc tggttacgca ccgcgcacgc cccgctgtta
     3121 atgtcgttgt ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta ctgttgcagt
     3181 tgttcccagg aggtggagca atcatccacg atgatcatct cccagttgct gtagtcctgg
     3241 cgcaggaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt tggcatatag
     3301 attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc tggtttgctt
     3361 gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc aggcgtcgtt
     3421 atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac gcgctggcgc
     3481 ggcggaagat ggagttcaga atcacccctt tcaccggaat acagttttgc tcaaagcggc
     3541 tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc attaacgtgg
     3601 ttccgacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc ggcgtatcaa
     3661 tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag cgttcgctca
     3721 ttaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca aatttggcaa
     3781 tagaggtcgg tttagcagcg gtagtaatat cgccctgacc aatcagaatc tccgacagcc
     3841 cattgacatt attggtgccc aacagctcgt gggtgtagcc tttgcgcata tcgcagtcga
     3901 tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg cagacaaagg
     3961 ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc gcctgcatca
     4021 tcgcgaagtg cagactggta cgcagactac ggatggcttc aatcgccaga tcggttggat
     4081 tccccactgc cagtagctgg ctctgtttat agcgtttaac ccctttgatg gttttgacgc
     4141 tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg attccgtgtt
     4201 cttccagcac ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc agcacaccca
     4261 cgatagagag catcaggcca aggataatcg cgccgaggat aatcagccct ttcttcggtt
     4321 ttagcacgcc aggctgggtg attgccgggt caataatgcg cacatcgccg acggtgctgg
     4381 cttcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc tgctgaccag
     4441 actcgacatc gcgggtcaga cggacaattt cctgctggtt tttcggcatc gccgttacgc
     4501 gaccgttaag tttggctttc tcttcttcca gcgcctgacg tttctccagc agcgtgcggt
     4561 acgccgggtg aactttggtg tacagcttgg agatttccgc ctctttaaag gtcagttcgt
     4621 tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttca agcggcagat
     4681 caacagaatc tttatcctga cggaaggcat tcagtttgtt ttcggcaaca tcgaggcggc
     4741 tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct tccgccgatt
     4801 tgcgctcaat attttgttcc tgatagttac gagcgatgct gttaagaatg tcgcggatct
     4861 gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg ccgttctccg
     4921 ttaccgtcag gctgttttgc agttggttga tcatccccaa cgtggagtat ttggtgacgg
     4981 taaactcact gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg ccttcttttt
     5041 tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc atcactgctc agggtgtagt
     5101 ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc gggcggttaa
     5161 aggtagtcac tttcaccgtc tcgttctgac gccccatcag gcgatcccag cccgccccga
     5221 aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc accgttttac
     5281 caagcaccag acgcgaacga atcaactgga tctcggcgtc cgatgcaggc ggtttgttgg
     5341 ctaatgccga gccgatatcc tgcaccagcg aattgccgct gttttgctcg atttgtacca
     5401 gtgcgtcggc actataaatc ggcgtggcga agaaagtgta aaccacggca cagagggcaa
     5461 atacgacggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg accaggcgac
     5521 caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact ttttctgtca
     5581 ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc gttcaagtaa
     5641 tgtgtacacc gctgcaaacg tttcccggct tttgtgatac ggatcgggga tttcacattc
     5701 gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca tctcgcataa
     5761 gcgttcgata tggcgcttat ccatggtcaa aatcaggtca tagttgcgac acaggcggcg
     5821 gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg cgacgctgat
     5881 agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact ccactttcag
     5941 ttccgggtga taacgttgca gtaagcgttc cgccatcggg gaacggcaaa tattgccgac
     6001 acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc gctggctgta
     6061 tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag acggttccag
     6121 cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg gaattctgtc
     6181 gccatcacca gcgacgtcgc atcggacata tccagctggt agatattggc aatcttgccg
     6241 ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc gttggaggtg
     6301 gtcatgtcga taccttcggc attggccaga gcttcagtca gggtcatgcc gctaaagtcc
     6361 attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag atcatcatta
     6421 cgcggcacgt agagaatatc gccgggataa agcagtcggt tctgattgag gtcgccgttt
     6481 tgcatcagcg cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag caccacgttg
     6541 cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag aatggtcagt
     6601 ggtacgttgg tgatcgcctg ttgaccggat ttattcacct gacctgagat ataggccttt
     6661 tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta cgtcgctaag
     6721 cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg aaccttgccg
     6781 atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc gctggagctg
     6841 cggtactgac cagctggcgt ggtgagttcc gggtgatccc agacggtgac attaagaacg
     6901 tcccccggcc cgacgcgata ctggtaattc gcgatctcgc tttccagcgt catattgggg
     6961 cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag cggataaaca
     7021 ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc tttgcccatc
     7081 gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac tgacaccaat
     7141 aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg gcagagtaat
     7201 tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag ttaattgaat
     7261 aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct attgacagaa
     7321 taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt tttagctacc
     7381 aatacactga ttgagcttaa tttttcaaac cctctctgca tacaatcgtt gatgagaaag
     7441 ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg aatattggct
     7501 cgccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg taacaattcg
     7561 gcaattggct atttttaaga attatattaa gtatcattca atatggtttt taggagtttc
     7621 tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa ataatgtttt
     7681 attattaccg cttttaattc agggataatg tgaggttatt accctcaaat aatatgagat
     7741 aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat tgtgacattt
     7801 gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg
     7861 gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt
     7921 attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg ggttaccggg
     7981 ctgctgctgg cgatgttaat gcgcctgtta ctgctagcgt caatctcctg gctggtcacc
     8041 ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga cttaatcatg
     8101 ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga acggctggaa
     8161 gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg cgtggtgacg
     8221 caaattgtgg tactggacgc cattttctca ctcgactcag tgattaccgc cgtcgggatg
     8281 gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct gatgttgatg
     8341 gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgctta
     8401 agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg
     8461 aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcact caatcagttg
     8521 gctatcttta accggcgacg ttttctttcc gctaatcaga cgctacgcca gcggactacc
     8581 gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga cgccaaaacc
     8641 gcgtccatgc tggtggatca tggaaaccag cagatcttta atccgcagga acggcggatg
     8701 attgagcggg tacttaatct taaccagcgt accgtaagca gcattatgac gtcgcgccac
     8761 gatattgagc atatcgatct caacgcgccg gaagaagaaa tccgccagtt gctggagcga
     8821 aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt gctcggtgtt
     8881 gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa cctgcgggtg
     8941 ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttaccagc cctggagcag
     9001 ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc ggtggaaggg
     9061 attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa
     9121 gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt
     9181 catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga aaaacgtgaa
     9241 tatcacacca ttgccgggct gttgatggag tatttgcagc gtattccaaa acctggcgaa
     9301 gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca tcgcgtgcag
     9361 aaggtgcaga ttataccgct gcgtaaggat ggcgagatgg agtacgaggt gtgatggtat
     9421 cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag cgttgtctgt
     9481 ggtaaacgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa tcgtttcctg
     9541 taggctgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc
     9601 gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc ggcgatttac
     9661 cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac gttcaccggc
     9721 ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg atcttacata
     9781 ttcagaatat aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg tcttatcagg
     9841 cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc acatctttgc
     9901 cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc gcttcgtcct
     9961 gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc tgccagttgc
    10021 cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg ctttccccgt
    10081 tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct gccagattca
    10141 acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg tttaacgtca
    10201 cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc acgttatcga
    10261 agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga atcatctgct
    10321 gggagttcat cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt ccttgccagt
    10381 tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc agtgacattt
    10441 ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc tcaacgttct
    10501 ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc gtgcccggca
    10561 gtgaaacctt tccaccgtta agcttgccct gcagttgagt aatttccagc aaacccgact
    10621 tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc acgttactgg
    10681 cctgcaacaa aatatcagct gtaaagcctt gcagtccctg ataggccggt tcatcaatac
    10741 gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc gcggcaccgt
    10801 tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt tgcgggaatt
    10861 gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt ccgctcagcg
    10921 tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt gactcctgcc
    10981 actgggactg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg gcaccctgca
    11041 actgccagtt aatttgttca atagccgctg ttaaatcatg cggataatca gaagcatcta
    11101 ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc ccggagaact
    11161 caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg cggattgtca
    11221 cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta agactggata
    11281 tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg cgcggtgcaa
    11341 ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc actgcccctt
    11401 ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc gccacgtcca
    11461 gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg agagacattc
    11521 gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg tcgagctgca
    11581 attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa tcattcggat
    11641 tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaagagt atcatcagcg
    11701 tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta tcgattcggc
    11761 tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg taaggtcgcg
    11821 ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc ggacgcagcg
    11881 ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac cagcccggat
    11941 cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag gaacgcccgt
    12001 ccagccagcc caccagatcg gctggcagcg tcaccgactc cagtgtcacc gccagcgcca
    12061 gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca tcgctcatca
    12121 cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata aacgctgccg
    12181 tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc gcgccgttaa
    12241 tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc caggcttcaa
    12301 tatctcggtc acacagacgc atggcatttt ctcctttcgc acatcactcc cttaacgcca
    12361 attacgtcaa gggcatacca acttatcact gaacggtaca caatttatca agcttattca
    12421 aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt cccgccgcgc
    12481 ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa catcgggcgc
    12541 acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc gttaacgtca
    12601 cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat ggagaagtta
    12661 agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat gatgaccttc
    12721 ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct gtaaaccggc
    12781 aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcaacag gctgtgatcc
    12841 atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag atggctttga
    12901 tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac ttgctcacgt
    12961 aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc gccagcgata
    13021 ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg attaacctgg
    13081 atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt catccgtctg
    13141 ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag aattcccggt
    13201 ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag ttgcgcaaat
    13261 tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac ttttgtacta
    13321 gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg taataagcaa
    13381 acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg tcagtttagg
    13441 tttagtctcg tttatcttta cccttgtctc gcttgagctt tcgcagtttg gcacccaact
    13501 cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc atgccgggcg
    13561 catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat ccattccagg
    13621 aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc cagttcaaag
    13681 ttgcattagc acaaacccag ggccaactgg caaaagataa agccacgctt gctaacgccc
    13741 gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc
    13801 tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa
    13861 gcgtcgccag cgcgcagttg caactcgact ggagccgcat caccgcacca gtcgatggtc
    13921 gcgttggtct caagcaagtt gatgttggta accaaatctc cagtggtgat accaccggaa
    13981 ttgtggtgat cacccagacg catcctatcg atttagtctt taccctgccg gaaagcgata
    14041 tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa gcctgggatc
    14101 gcaccaattc gaagaaatta agtgaaggca cgctgttaag cctcgataac caaatcgatg
    14161 ccacaaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcgctgtttc
    14221 ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc
    14281 caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa
    14341 acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc
    14401 gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag
    14461 gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac tccggaagag aaagccacca
    14521 gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc cgagcagcac
    14581 aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc tgatggtggc
    14641 gatcttactc gccgggatta tcggttatcg cgccctgccc gtttcggcgc tgccggaagt
    14701 ggactatccg accattcagg tggtcacgct ctacccaggt gccagcccgg atgtcatgac
    14761 ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag atgtctggcc tgaaacagat
    14821 gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc taacattacc
    14881 gctcaatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca acttgttgcc
    14941 gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc cgccgatcat
    15001 gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata tggtggaaac
    15061 ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg
    15121 tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc tcggcctgac
    15181 cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa aaggtagcct
    15241 cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga
    15301 gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg gcgatgtcgc
    15361 aactgtagag caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc
    15421 cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg ccgacagcat
    15481 tcggcagatg ctgccacagc tcactgagag tctgccgaaa tcggtgaagg tgacagtgct
    15541 ttccgatcgc accaccaata tccgcgcatc cgtcgatgat actcagtttg aattgatgat
    15601 ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc cggcgaccat
    15661 cattcccggt gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta tggtgtttct
    15721 cgatttttca atcaataacc tgacactgat ggctttaact atcgccaccg gattcgtggt
    15781 cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc
    15841 gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct cgctgacctt
    15901 ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg ggcgactgtt
    15961 ccgcgaattt gctattaccc tggcggtagc gattttgatc tcagcggtgg tgtcgctgac
    16021 cctgacaccg atgatgtgcg cgcggatgct cagccaggag tcgttgcgta aacagaacag
    16081 cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg gtcgtggact
    16141 ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg gcactcagca cgctgctgct
    16201 tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc ccggtacagg acaacggcat
    16261 tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg cccagcgaca
    16321 acgccaggtc gcggacgtga ttttgcagga tccggcagtg caaagcctga cctcatttgt
    16381 tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc
    16441 gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtaga
    16501 taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gacctgacca tcgatactca
    16561 ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg cgctcagtac
    16621 ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg atgtctccag
    16681 cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg
    16741 tctggggatc agcatggcgg acgtcgataa cgccctgtac aacgcgtttg gtcagcgact
    16801 gatttccact atttatactc aggctaacca gtatcgcgtg gtgctggagc acaataccga
    16861 aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg acggtggcgt
    16921 ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgcttt ccatcaacca
    16981 tctggatcag ttcccggtaa caaccatctc ctttaacgtg ccggataact attcgctggg
    17041 cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg tggatatcac
    17101 cacgcaattc tagggcagca ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct
    17161 gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg agagctttat
    17221 tcacccgatc accattctct cgacgctacc caccgcaggg gtcggcgcgc tgctggcatt
    17281 aatgattgcc ggtagcgaac tggatgtgat tgcgattatc ggcattattt tgctgatcgg
    17341 tattgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg agcgcgagca
    17401 aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc gtccgatcct
    17461 gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta ccggggtcgg
    17521 cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgattg tcagccaggt
    17581 gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg cattgtggac
    17641 caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg aagttttttg ccctcttcat
    17701 ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg gcatactggg
    17761 tttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga ttatggtcag
    17821 cgcctcgctg cccggtgcgt cgccggagac aatggcgtct tccgttgcca cgccgctgga
    17881 gcgctcactt gggcgcattg ccggggttag tgaaatgacc tccagcagtt cgctcggcag
    17941 cacgcgcatt attttgcagt ttgattttga ccgggatatc aacggcgcag cgcgtgatgt
    18001 gcaggcggcg atcaacgctg cacaaagttt gctgcccagc gggatgccca gccgcccgac
    18061 ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctcacgctga catccgatac
    18121 ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag ctggctccga cgatttcgca
    18181 aatcgacggt gtcggtgatg tcgatgtcgg tggcagctcg ctgcccgccg tgcgcgtcgg
    18241 gctgaccccg caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac gcactgccat
    18301 cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc accgctggca
    18361 gatccagacc aacgatgaac tgaaaaccgc cgctgaatat cagccgctga ttattcacta
    18421 caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgactgact cagtgcagga
    18481 tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttactgatga tccgcaaact
    18541 gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac cagagttgca
    18601 ggagaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc ccaccattcg
    18661 cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg tgattctggt
    18721 ggtcttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg cggtgccggt
    18781 ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca ataatctttc
    18841 gctgatggcg ctcaccattg ctactggttt cgtggtggat gacgccattg tggtgctgga
    18901 aaacattgcc cgtcatctgg aagcggggat gaaaccacta caggccgcgc tgcaaggtac
    18961 tcgcgaagtc ggctttacgg tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc
    19021 gctgctgttg atgggcggat tgccgggccg actgttacgc gagtttgccg tgacgctttc
    19081 tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta acgccaatga tgtgcggctg
    19141 gatgctaaaa gccagcaagc cgcgtgagca aaagcgactg cgtggttttg gccgcatgtt
    19201 ggtagccctg caacaaggct acggcaagtc gctgaaatgg gtgctcaatc atacccgtct
    19261 ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tcgatcccga aaaccttctt
    19321 cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga gtatttcgtt
    19381 tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg atccggcagt
    19441 ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga tgtttatcac
    19501 cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc gtctgcgcgt
    19561 gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag atattcgcgt
    19621 tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg acctggcggc
    19681 actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg aactggcgga
    19741 cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg accgcgacac
    19801 catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata acgccttcgg
    19861 tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg tgatggaagt
    19921 ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta tcaataacga
    19981 aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg ccccactatc
    20041 ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc cgaccggaaa
    20101 atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg gtgtgccttc
    20161 gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga tgaactcgca
    20221 ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta tcctttacga
    20281 gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg ttggagcgct
    20341 gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag ggatcatgct
    20401 attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc ttgaagccca
    20461 acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc tgcgttttcg
    20521 cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg tattgtcggg
    20581 cggtgacggc tcggagctgc ggcaacccct ggagatcacc attgtcggcg gtctggtgat
    20641 gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg accgtctgcg
    20701 gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag atcttcccga
    20761 cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt cgctggacac
    20821 caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa gtccgttgca
    20881 tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc ccgccagcgg
    20941 ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg tgctgtttac
    21001 tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc tggcacgcgc
    21061 gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg tgatgaaaat
    21121 cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg gtcaggtcgg
    21181 tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt ggcactggat
    21241 ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattga tgttaatgcc
    21301 gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc tggcggttgg
    21361 catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat cgccgctggc
    21421 gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc tgcacgccag
    21481 aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct tttcgctggg
    21541 cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta tgacaccggt
    21601 tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga tgatcccgat
    21661 ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc gctttggtta
    21721 tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt tgtttatgac
    21781 taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac aagggatggt
    21841 caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc cggacaatct
    21901 ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga gtatcggcgt
    21961 caccatcgcc gggctgttgc tggggctttt tggttcgcag catatcagcg tcgacagcgg
    22021 caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca tcgcccttcc
    22081 ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta tttcgcggcg
    22141 aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact gtttctggcg
    22201 attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg tatcagtttt
    22261 gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca gctgttaagt
    22321 gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg caacaacgat
    22381 cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga taaacccggc
    22441 ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca aaacaacaaa
    22501 gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc cattctggtc
    22561 aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac ccgcaatact
    22621 gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc cctggcaacg
    22681 ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc ggtaaaacga
    22741 cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt aacgcccacc
    22801 agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag cacgctggag
    22861 aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact gcgtacgcca
    22921 ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa attcacgccg
    22981 gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt tgacgatctt
    23041 catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc ggtagatttg
    23101 atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag ccgtggcctg
    23161 aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga ccgtttaatg
    23221 cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg cggcagcctg
    23281 aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga cagtgcgcca
    23341 ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac cgaaggttcc
    23401 cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa cattgttgaa
    23461 gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag cattacagta
    23521 gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca atcgacgaaa
    23581 acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg ctcattgatt
    23641 atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag gtactggcgt
    23701 atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct ggcaccgatg
    23761 gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg atggtgacgg
    23821 caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat gactacatct
    23881 gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg cgccgttgta
    23941 aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc gacgaaggtc
    24001 gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa tttcgtctgc
    24061 ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg ctcaatcatc
    24121 tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt aaaaatctgc
    24181 gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt tacggcgttg
    24241 gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat tactttgtta
    24301 gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt cagcaaactg
    24361 acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat cagtaaacag
    24421 cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat ggctggttgg
    24481 tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt
    24541 gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa gggcatcgcg
    24601 tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc aagcaacggc
    24661 aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag ccagatgtac
    24721 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
    24781 acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt ccggcgcttt
    24841 accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct cccctaaccg
    24901 cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg tttaaaccgg
    24961 aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag
    25021 atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac gaattcaacc
    25081 acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg
    25141 tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg
    25201 tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc
    25261 gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc gtgaactggg
    25321 cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct cgcgagctgt
    25381 cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc gagatcttcg
    25441 ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca
    25501 agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac aacgtccagg
    25561 aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa
    25621 atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag
    25681 cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga
    25741 actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt
    25801 cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc gcacaggttt
    25861 accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc ctgctggaaa
    25921 ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg
    25981 acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg
    26041 agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat aaattctccg
    26101 ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc cttgagcaca
    26161 tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat actgtgtggc
    26221 tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat ttctccgggg
    26281 aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc ggatggaaaa
    26341 ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa
    26401 aacataatcc ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc
    26461 cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc tgataaccgt
    26521 cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc acgtaacggt
    26581 atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg acgcttacaa
    26641 aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac
    26701 cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg
    26761 catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa gaagcttcgg
    26821 cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa
    26881 atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa
    26941 ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac caacgacact
    27001 ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat tataataaat
    27061 gttctgtaca acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa
    27121 ctccatgggg gctttaatat attatattga agatgccact gtttagttga atattaggta
    27181 tatgctcttt tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag
    27241 ctcctggtgg taaccactga atcctccata cttgaactga ctttttatcc tccgactttc
    27301 atcctgttct gactccacct tttgttttct gctctacact atctacagac caatcataaa
    27361 ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg gtaaaagtac
    27421 tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca
    27481 tgtgcgggtc acctgggaga aaggtgatgc cgcacgattt gtagaggagg cccggaagtt
    27541 gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc
    27601 gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat taggaaccgc
    27661 caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc
    27721 aattgccggt aatgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat
    27781 taatatggcg acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa
    27841 agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca
    27901 accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat
    27961 tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga acgcgttaat
    28021 taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt
    28081 atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt
    28141 tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagtgggca
    28201 aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtcc
    28261 attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa tgccggatgc
    28321 ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg
    28381 tcttctgcaa tgccaattta tccacctctg acacagcatc atcggtaaca ataatgtcaa
    28441 ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat gaatcgagaa
    28501 cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa ccttcattaa
    28561 aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat
    28621 taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga
    28681 ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg
    28741 ggagactgtt ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca
    28801 ttgtggtact tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg
    28861 cctgcgctat tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt
    28921 cctgggtctc ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg
    28981 taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag
    29041 caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga
    29101 tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg
    29161 taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc cagcatcacg
    29221 ctggcccgcg gtttcatgga atgagcaaca ctttgccctg catggcatta cgagcgatgt
    29281 cacgcaccgc ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc
    29341 ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc
    29401 tggaatagtt catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg
    29461 ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag
    29521 gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa
    29581 gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac
    29641 tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct gagctaatgt
    29701 cgatcgccgt cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa
    29761 tggttccggc accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag
    29821 catgcaggcc aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg
    29881 gtagagcaaa gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc
    29941 gcgagccaat aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggac
    30001 aagtaaaaca gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa
    30061 caccggatcc caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat
    30121 aatgtgcacc atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa
    30181 tttttacccg cacctcatcc tgatgtttaa tttcaggaat gacgcttttt gcaacgcgca
    30241 cgataccatc agtatcattc actactgatt tcataaaaac tcctgatt
//
LOCUS       DAJTRM010000043        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRM010000043 DAJTRM010000000
VERSION     DAJTRM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.043"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO322_002040"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO322_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5906926.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO322_002041"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO322_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5906927.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO322_002042"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO322_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5906928.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO322_002043"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO322_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5906929.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO322_002044"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO322_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5906930.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO322_002045"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO322_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5906931.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO322_002046"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO322_002046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5906932.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO322_002047"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO322_002047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5906933.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO322_002048"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO322_002048"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5906934.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO322_002049"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO322_002049"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5906935.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO322_002050"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO322_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5906936.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO322_002051"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO322_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5906937.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO322_002052"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO322_002052"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5906938.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO322_002053"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO322_002053"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5906939.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO322_002054"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO322_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5906940.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO322_002055"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO322_002055"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5906941.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO322_002056"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO322_002056"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5906942.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO322_002057"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO322_002057"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5906943.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO322_002058"
     CDS             21622..22212
                     /locus_tag="OO322_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5906944.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO322_002059"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO322_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5906945.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO322_002060"
     CDS             22684..23379
                     /locus_tag="OO322_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5906946.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO322_002061"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO322_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5906947.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO322_002062"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO322_002062"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5906948.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO322_002063"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO322_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5906949.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO322_002064"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO322_002064"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5906950.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO322_002065"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO322_002065"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5906951.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7027 a         7823 c         8256 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgagg aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTRM010000044        29403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRM010000044 DAJTRM010000000
VERSION     DAJTRM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.044"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..441)
                     /locus_tag="OO322_002066"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO322_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO322_002067"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO322_002067"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5906952.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO322_002068"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO322_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5906953.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO322_002069"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO322_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5906954.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO322_002070"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO322_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO322_002071"
     CDS             5754..6257
                     /locus_tag="OO322_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5906955.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO322_002072"
     CDS             complement(6337..6588)
                     /locus_tag="OO322_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5906956.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO322_002073"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO322_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5906957.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO322_002074"
     CDS             7052..7375
                     /locus_tag="OO322_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5906958.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO322_002075"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO322_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5906959.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO322_002076"
     CDS             complement(8080..8319)
                     /locus_tag="OO322_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5906960.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO322_002077"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO322_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5906961.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO322_002078"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO322_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5906962.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO322_002079"
     tRNA            complement(10643..10729)
                     /locus_tag="OO322_002079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO322_002080"
     tRNA            complement(10742..10815)
                     /locus_tag="OO322_002080"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO322_002081"
     tRNA            complement(10870..10945)
                     /locus_tag="OO322_002081"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO322_002082"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO322_002082"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5906963.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO322_002083"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO322_002083"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5906964.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO322_002084"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO322_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5906965.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO322_002085"
     CDS             14483..14659
                     /locus_tag="OO322_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5906966.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO322_002086"
     CDS             14646..14870
                     /locus_tag="OO322_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5906967.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO322_002087"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO322_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO322_002088"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO322_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5906968.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO322_002089"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO322_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5906969.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO322_002090"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO322_002090"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5906970.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO322_002091"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO322_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5906971.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO322_002092"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO322_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5906972.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO322_002093"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO322_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO322_002094"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO322_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5906973.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO322_002095"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO322_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5906974.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO322_002096"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO322_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5906975.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO322_002097"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO322_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5906976.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO322_002098"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO322_002098"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5906977.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO322_002099"
     CDS             complement(26368..26781)
                     /locus_tag="OO322_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5906978.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO322_002100"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO322_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5906979.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO322_002101"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO322_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5906980.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO322_002102"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO322_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29403)
                     /locus_tag="OO322_002103"
     CDS             complement(29220..>29403)
                     /locus_tag="OO322_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5906981.1"
                     /translation="ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         7321 a         7587 c         6997 g         7498 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagctaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tct
//
LOCUS       DAJTRM010000045        29323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRM010000045 DAJTRM010000000
VERSION     DAJTRM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.045"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..617
                     /locus_tag="OO322_002104"
     CDS             <1..617
                     /locus_tag="OO322_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5906982.1"
                     /translation="CVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFN
                     NLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITM
                     LGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHH
                     GIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            639..2048
                     /locus_tag="OO322_002105"
     CDS             639..2048
                     /locus_tag="OO322_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5906983.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2026..2805
                     /locus_tag="OO322_002106"
     CDS             2026..2805
                     /locus_tag="OO322_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5906984.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2802..3662
                     /locus_tag="OO322_002107"
     CDS             2802..3662
                     /locus_tag="OO322_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5906985.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3810..4385)
                     /locus_tag="OO322_002108"
                     /pseudo
     CDS             complement(3810..4385)
                     /locus_tag="OO322_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4402..4662)
                     /locus_tag="OO322_002109"
     CDS             complement(4402..4662)
                     /locus_tag="OO322_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5906986.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4653..5924)
                     /locus_tag="OO322_002110"
     CDS             complement(4653..5924)
                     /locus_tag="OO322_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5906987.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6002..6364)
                     /gene="queD"
                     /locus_tag="OO322_002111"
     CDS             complement(6002..6364)
                     /gene="queD"
                     /locus_tag="OO322_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5906988.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6683..8482
                     /gene="cysJ"
                     /locus_tag="OO322_002112"
     CDS             6683..8482
                     /gene="cysJ"
                     /locus_tag="OO322_002112"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5906989.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8482..10194
                     /gene="cysI"
                     /locus_tag="OO322_002113"
     CDS             8482..10194
                     /gene="cysI"
                     /locus_tag="OO322_002113"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5906990.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10268..11002
                     /gene="cysH"
                     /locus_tag="OO322_002114"
     CDS             10268..11002
                     /gene="cysH"
                     /locus_tag="OO322_002114"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5906991.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11266..11418
                     /locus_tag="OO322_002115"
     CDS             11266..11418
                     /locus_tag="OO322_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5906992.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11612..12703
                     /locus_tag="OO322_002116"
                     /pseudo
     CDS             11612..12703
                     /locus_tag="OO322_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12849..13886)
                     /gene="iap"
                     /locus_tag="OO322_002117"
     CDS             complement(12849..13886)
                     /gene="iap"
                     /locus_tag="OO322_002117"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5906993.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14138..15046
                     /gene="cysD"
                     /locus_tag="OO322_002118"
     CDS             14138..15046
                     /gene="cysD"
                     /locus_tag="OO322_002118"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5906994.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15048..16475
                     /gene="cysN"
                     /locus_tag="OO322_002119"
     CDS             15048..16475
                     /gene="cysN"
                     /locus_tag="OO322_002119"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5906995.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16475..17080
                     /gene="cysC"
                     /locus_tag="OO322_002120"
     CDS             16475..17080
                     /gene="cysC"
                     /locus_tag="OO322_002120"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5906996.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17130..17453
                     /locus_tag="OO322_002121"
     CDS             17130..17453
                     /locus_tag="OO322_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5906997.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17647..17958
                     /gene="ftsB"
                     /locus_tag="OO322_002122"
     CDS             17647..17958
                     /gene="ftsB"
                     /locus_tag="OO322_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5906998.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17977..18687
                     /gene="ispD"
                     /locus_tag="OO322_002123"
     CDS             17977..18687
                     /gene="ispD"
                     /locus_tag="OO322_002123"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5906999.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18687..19166
                     /gene="ispF"
                     /locus_tag="OO322_002124"
     CDS             18687..19166
                     /gene="ispF"
                     /locus_tag="OO322_002124"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5907000.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19163..20212
                     /gene="truD"
                     /locus_tag="OO322_002125"
     CDS             19163..20212
                     /gene="truD"
                     /locus_tag="OO322_002125"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5907001.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20193..20954
                     /gene="surE"
                     /locus_tag="OO322_002126"
     CDS             20193..20954
                     /gene="surE"
                     /locus_tag="OO322_002126"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5907002.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20948..21574
                     /gene="pcm"
                     /locus_tag="OO322_002127"
     CDS             20948..21574
                     /gene="pcm"
                     /locus_tag="OO322_002127"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5907003.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21714..22853
                     /gene="nlpD"
                     /locus_tag="OO322_002128"
     CDS             21714..22853
                     /gene="nlpD"
                     /locus_tag="OO322_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5907004.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQLVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22916..23908
                     /gene="rpoS"
                     /locus_tag="OO322_002129"
     CDS             22916..23908
                     /gene="rpoS"
                     /locus_tag="OO322_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5907005.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24028..24435)
                     /locus_tag="OO322_002130"
     CDS             complement(24028..24435)
                     /locus_tag="OO322_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5907006.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24582..25175
                     /locus_tag="OO322_002131"
     CDS             24582..25175
                     /locus_tag="OO322_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5907007.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25175..26122
                     /locus_tag="OO322_002132"
                     /pseudo
     CDS             25175..26122
                     /locus_tag="OO322_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26395..26958)
                     /locus_tag="OO322_002133"
     CDS             complement(26395..26958)
                     /locus_tag="OO322_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907008.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27123..27374
                     /locus_tag="OO322_002134"
     CDS             27123..27374
                     /locus_tag="OO322_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5907009.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27376..>29323
                     /locus_tag="OO322_002135"
     CDS             27376..>29323
                     /locus_tag="OO322_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5907010.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPW"
BASE COUNT         7144 a         7283 c         7949 g         6947 t
ORIGIN      
        1 aatgtgtgct gatctttatg gctgaaggta accctcgcat tgcgaaggcg acgggcgaag
       61 ggattgcgga aatcgttgcc cgctacccgc agtgccagcg cgtggacagc aaactgattg
      121 aaacctggtt taacaacctg aactggggac cggataaagt ggctgccgaa cgtgtgcaga
      181 tcctcaaaac cggcaacatg ggctttacca ccgaagtgtc cggctgctgg agctgcatcc
      241 acgaaatcta cgaaagcgtt attaaccgta ttcgtactga gttcccgcac gccgacgaca
      301 tcaccatgct gggcggtcac tcctctcata gctatcagaa cggcaccaac atgtacttcg
      361 tctatgacta caacgttgtt gactgtaagc cggaagagga aatcgacaag taccacaatc
      421 cgctcaacaa aatcatctgc gaagaaacca ttcgcctcgg cggttcgatg gtgcaccacc
      481 atggcattgg taaacatcgc gttcactgga gcaagctgga acacggcagc gcgtgggcgt
      541 tgctggaagg gctgaaaaag cagttcgatc ctaacggcat tatgaatacg ggtactatct
      601 atccgattga aaaataatgt atcaggcagc gttccgcgat gacggggcgt tgccttttcg
      661 gcttctcagg cgagaagccg ttcttattac cggacaatga aggggtaaag atgaacactt
      721 caccggtgcg aatggatgat ttaccgctta accgttttca ctgtcgaatt gctacgctca
      781 ctttcggcgc acacctgacc gacggttatg ttctaggcgt cattggttac gccattattc
      841 aacttacgcc cgccatgcaa ctgacgccgt ttatggcggg aatgatcggc ggctcggcgc
      901 tccttggttt gtttcttggc agcctggttc ttgggtggat ctccgaccat attggtcggc
      961 aaaaaatctt caccttcagc tttttgctga ttacgctcgc ttcgttcttg caattttttg
     1021 ccaccacgcc agagcatctt attgggctgc gcattttgat cggcattggt ctgggaggcg
     1081 attactcagt aggtcacacc ttgctggctg aattttcccc gcgccgccat cgcggtattt
     1141 tgctgggcgc attcagcgtg gtgtggaccg taggctatgt gttggcaagt attgccggac
     1201 atcactttat ttccgaaaac ccggaggcct ggcgctggtt gctggcatcg gcagctctgc
     1261 ccgcgttgtt gattacgtta ttacgctggg gaacgccaga atcgccacgt tggctactgt
     1321 gccaggggcg ttttgcagaa gctcacgcta tcgtgcatcg ctattttggt ccccatgttt
     1381 tactgggcga tgaagtggta acggcgaccc ataaacacat caaaaccttg ttctcttcgc
     1441 gttactggcg gcgcacggcg tttaacagcg tcttctttgt ctgcctcgta atcccatggt
     1501 ttgtgattta tacctggctg ccaactatcg cccagactat tggtctggaa gatgcgctga
     1561 ctgccagcct gatgcttaat gcgttgttaa ttgtgggcgc gctgctggga ttagttctga
     1621 cgcacctgct ggcacatcgc aaatttttgc tgggaagttt tttgctgctg gcggcaacgc
     1681 tggtagtaat ggcctgtttg ccttccggca gttcattaac gctgctgctt tttgttctct
     1741 tcagcaccac catttcggca gtcagtaatc tggtgggcat tttgcctgcg gaaagttttc
     1801 ctactgacat tcgctcgctg ggagtcggtt ttgccaccgc catgagtcga cttggcgcgg
     1861 cggtaagtac tggcctgctg ccgtgggtgc tggcgcagtg gggaatgcaa gtcaccttat
     1921 tgctcctagc gacagtgttg ttggttggtt ttgttgtgac ctggctatgg gcaccagaaa
     1981 ctaaagccct cccgctggtg gcggcgggaa atgtaggagg tgcgaatgaa cattctgtta
     2041 gcgtttaaag ccgaaccgga tgccggaatg ctggcggaaa aagagtggca ggcggcggct
     2101 cagggtaata gcggaccgga tgtttcgcta ctgcgaagtt tactcggtgc tgatgaacag
     2161 gccgctgccg cgctgctgtt agcgcagaga aaaaacggta cgccgatgtc tttaaccgcg
     2221 ttgagcatgg gggatgaacg ggcgttgcac tggctgcgct atctcatggc tcttgggttt
     2281 gaggaagctg tattgctgga gacggcggca gatctgcgct ttgcgccgga atttgtcgct
     2341 cgccatattg ccgaatggca gcgtcagaat ccgctggatc tgataattac cggcagccaa
     2401 agcagcgaag ggcagaatgg gcaaacgcca tttttgctgg cggaaatgct gggctggccc
     2461 tgcttcaccc aggtggagcg tttcactctc gacgcgctgt ttatcaccct cgaacagcgt
     2521 actgaacatg ggttgcgctg ttgccgggtt cgcttgcctg cggtgattgc cgtgcgtcag
     2581 tgcggtgaag tggccttgcc agtaccggga atgcgccagc gaatggctgc ggggaaagca
     2641 gaaattatcc gtaaaactgt tgctgccgaa atgccagcga tgcagtgtct gcaactggca
     2701 agagcagagc aacgacgcgg agcgacgctg attgatgggc aaacggtagc agaaaaagct
     2761 caaaagctat ggcaggacta tttacgccaa aggatgcagc catgaatatc gcaattgtga
     2821 caataaatca ggagaatgcc gcaatagcga gctggctggc ggcgcaggat ttcagcggtt
     2881 gtacgctggc gcactggcaa atagaacctc agcccgtagt ggcggaacag gtgctggatg
     2941 cgatggtgga acagtggcag cgaacgcctg cggacgttgt tctctttccg ccgggaacgt
     3001 ttggcgatga actgtcgacc cggctggcct ggcgtttaca tggtgcaagc atctgtcagg
     3061 tgacttcgtt agatattcca accgtaagca tgcgcaaatc ccactgggga aatgcgttaa
     3121 cggcgacgct gcaaacagaa aaacgcccgt tatgcctttc cctggcgcgt caggcgggag
     3181 cagcgaaaaa cgccacgttg ccctctggaa tgcagcaact gaacattgtc ccaggtgcgc
     3241 ttcccgactg gctggttagc acagaggatc tgaaaaatgt tagccgcgac ccattagctg
     3301 aggccagacg ggtgctggtg gtgggacagg gcggagaggc agataaccag gaaatcgtta
     3361 tgctggcaga aaagctggga gccgaagtgg gctacagtcg ggcgcgagtg atgaacggtg
     3421 gcgttgatgc tgaaaaagtg attggtattt ccggacatct gctggcacct gaggtgtgca
     3481 ttgttgttgg cgcttccggc gcagcagcgt taatggcggg cgtacgcaat agcaagttta
     3541 tggtggcgat taatcatgac gccagcgcag cggtattttc acaggcagat attggcgtgg
     3601 ttgatgactg gaaagtggtg ctggaggcac tggtgacaaa cattcacgca gaataccaat
     3661 aacgtgctgc cggatgatcc tgcctacggt tctggactgg ggacctgtag gcctgataag
     3721 acgcgttaag cgtcgcatca ggcgctgaat gtcggatgtg gcgtgcggcc aacaaatcaa
     3781 aacaaattca attggttacg attatcccgt caaagcaatt ttttcgctaa cgtccagact
     3841 ccggtattcg tggtggaaag cgccacgaca cccgcgttaa tcgcattacg cgcatcttct
     3901 tcatcgcata ccagcccgcc agcaatcagc ggttggcgga ttttttccgt cacccagccc
     3961 agcactttgg gcatgcagcc tggcaggatc tcaatacaat ccggattcga ttgcgcaact
     4021 tacttatcaa tgttgtgaaa cgaaatcgaa tcaacaataa acaggtgatg aatacagaaa
     4081 aaaacttccg ctcttgccgc ttttagcatc gatgctttgg tgctgataat tccatccgcc
     4141 tcggtcacca gtttcaaaaa ctgaatcacc acttctttat ttgacgcgcc ttccagcaaa
     4201 tcaacatgaa taaaagcata tttcccggcg ttcttaatct ttttaacgat attactgatg
     4261 gtgcagatat tgccgtacaa cacggaaata aactggcatt cagaatcgat ggccagttgc
     4321 aggctggcat tatctttaac ggcagcaatc accggattct ggcggagcag gtgtaagagg
     4381 ggcataaatt atccttatta tttatccaaa gcggtaggtg atgccgaatc cggaaggcgg
     4441 atagcgccac tgttgtagtg tggattcgtc gcacagcaaa cggcaggtgc cacactccag
     4501 gcagccgcga tagtcaatac gtaagtcacc ttcctgtgtg agagaaaaaa gacctaccgg
     4561 acaggcgtta atcaaccgtt gtaccatctg acgctcaacg gagtcagccg gaacaatgtg
     4621 cggcgcatca gcaacgcgcc agagattacg ggctacagac atcgcagact cctgataaca
     4681 tcgcccgcca gatgccacag gccatgacga cgtaaatgat gccagaataa ctggcgcagc
     4741 ggtggaacag gtttatcacc ctgatcccat aaatcgcggg aaatatcctg cattaacgca
     4801 ggccacgccc gatacctgcc agggcgttgc aaaagcgccg gaacatgctg ataacgttgt
     4861 agaacatccc atagcaggct gcgctctacg ttgtgatgat aaagcgcaaa cagattttgc
     4921 ggctcgcggt gctggcaggc gcttattagc gtttgtgccg ccgcctgtgc gccagtcagc
     4981 gccatatcca tgccgcgcac ggaaattccg gtattgacgc aactgcgcaa cgcatcgccc
     5041 accagcagcc agccgttacc ggcgtattgc accggcatac tgtgcaagcc accttctggc
     5101 accagatgcg caccatactc cagtgattcc gtgtttttga taagcgtgcg cactgccgga
     5161 tgcgctttaa agcgagtcag cagctcgctt gccggaacac gactttgcgt aagggaagag
     5221 agcgggcaaa caatccctaa cgagagcgtt tgttgattag tataaagaaa tgcgccgccg
     5281 ggtaagtcat cacagattcc gccgctgaac agcaacgctg cgccttcgtt attctccaga
     5341 tgaaaacgtt cttcaatagc ggatgtttcc agcgacagca cttctttgat ccccaacgcc
     5401 atcgcttcgc cagcaggacg agtcactaac ccgtgacgtt cagccaggac gctgttggca
     5461 ccttctgcca gcaccacata acgggcgcgg agaatatcgt caccacaaat aacgccacag
     5521 actctgccgt tttcttcata cagtgcatcc accgtcgctc cggggatgca ttcgacacct
     5581 tctttttcgg cttcggcaac cagccacgga tcgaaccgtg cacgtaatac attccaggat
     5641 tcaccgccgg gctgtaagct ggaaaacgtc gttgcgccat ccggcgttaa cagggaaagg
     5701 ctttcgtgag tgatgcgtcg ttcaagaggc gcggtcagat gaaattgcgg gaggagttcc
     5761 gcgagtgcat gggtatataa ccgcctgccg gaaagatttt tgctgccggg gatttcagcg
     5821 cgttcgagta acaaaacgga taaatccgct cgcgcgcagc gtaacgcgca agcggtgcct
     5881 gcaataccgg caccaataat aataatgtcg cagtcgtctt ccatgtcatg ccccagatat
     5941 ccagaacaca acaccctaac atagcgttac taaagggaaa ttgaccgccg acacactctt
     6001 atcattcgcc gcgatagata caccctgcgg tgcaggtttc ttttaccatc accgcactta
     6061 acagcggcac aacgggttta acctgatccc aaatccattt tgctaaaact tcgctggttg
     6121 gattttccag acctggaata tcattgagat aatggtgatc gaggcgctca taagttggct
     6181 taaacgccgc tttcagttca gcgaaatcga taatccagcc cgtatgcgga tcgacttccc
     6241 cggtaatttc cagtcgcacc ataaaggaat gcccgtgcag gcgaccacat ttatgccctt
     6301 ccgggacatg tggtaagcgg tgagcggctt cgaaggtgaa atctttaaat aacgtggtgg
     6361 acatcataat ttctctacag gacgataaaa ccgccgtaga gtaccggaaa ggctgttttt
     6421 tcgcattatc taaaacgact ttgttgcgca aaatcgctga tttatcttaa tgattggcta
     6481 aattcatttg tttttcatta ggttggttaa tctattttgt tgttaaagac tattgctaaa
     6541 acaggttagt cgatttggtt attagttatc gctatcccgt ctttaatcca caccgttcac
     6601 cccgttaacc ttaccttctc ttctgtttta tgggcgctga cagggcgcag aaacagcttt
     6661 gcttactgga acataacgac gcatgacgac acaggtccca ccttccgcgt tgcttccgtt
     6721 gaacccggag caactggcac gccttcaggc ggccacgacc gatttaacgc ctacccagct
     6781 tgcctgggtt tctggctatt tctggggcgt gctcaaccag cagcctgttg cgcttgcagt
     6841 gacgccagcg cccgccgcag aaatgccggg tataacgatt atctctgctt cgcaaaccgg
     6901 caatgcgcgc cgggttgctg aagcattacg cgatgattta ttagcagcaa aactgaacgt
     6961 taagctggtg aacgcgggcg actataaatt caaacaaatc gccagcgaaa aactgctcat
     7021 cgtagtgacg tcaacgcaag gggaagggga accgccggaa gaagccgtcg cgctgcataa
     7081 gttcctgttc tccaaaaaag cgccaaagct ggaaaacacc gcgtttgccg tgtttagcct
     7141 cggcgatagc tcttatgaat ttttctgcca gtccgggaaa gattttgaca gcaagctggc
     7201 ggaactgggg ggtgaacgcc tgctcgaccg tgtcgattct gatgttgaat accaggctgc
     7261 tgccagcgag tggcgcgccc gcgtggttga tgcgcttaaa tcgcgtgcgc ctgtcgcggc
     7321 accttcgcaa tccgtcgcta ctggcgcggt aaatgaaatc cacaccagcc cgtacagcaa
     7381 agacgcgccg ctggtggcta gcctctctgt taaccagaaa attactgggc gtaactctga
     7441 aaaagacgtt cgccatatcg aaattgactt aggcgactcg ggcctgcgtt accagccggg
     7501 tgacgcgctg ggcgtctggt atcagaacga tccggcactg gtgaaagaac ttgtcgaact
     7561 gctgtggctg acaggcgatg aacctgtcac tgtcgagggc aaaacgttgc ctctgaacga
     7621 agcgctacag tggcacttcg aactgaccgt caacaccgcc aacattgttg agaattacgc
     7681 cacgcttacc cgcagtgaaa cactgctgcc gctggtgggc gataaagcga agttacagca
     7741 ttacgccgcg acgacgctga ttgttgacat ggtgcgtttc tccccggcac agcttgatgc
     7801 cgaagcgcta attaatctgc tgcgcccgct gaccccgcgt ctctattcca ttgcctcctc
     7861 gcaggcggaa gtcgagaacg aagtacacgt caccgttggt gtggtgcgtt acgacgtgga
     7921 aggccgcgcc cgtgccggtg gtgcctccag cttcctcgct gaccgcgtgg aagaagaggg
     7981 cgaagtccgc gtatttatcg aacataacga taacttccgc ctgcccgcta acctggaaac
     8041 cccggtgatt atgattggcc caggcaccgg tattgcgccg ttccgcgcct ttatgcagca
     8101 acgcgccgct gacgaagcgc caggtaaaaa ctggctgttc tttggcaatc cgcattttac
     8161 ggaagacttc ctgtatcagg tggagtggca gcgctacgtc aaagagggtg tgctgacgcg
     8221 gatcgatctc gcctggtcgc gcgaccaaaa agaaaaagtt tacgtacaag ataaactgcg
     8281 cgaacagggc gcggagctgt ggcgctggat caatgacggt gcccacattt atgtctgcgg
     8341 cgacgctaat cgcatggcga aagacgttga gcaggcactt ctggaagtga ttgccgaatt
     8401 tggtggcatg gacaccgaag cggcggatga atttttaagt gagctgcgcg tagagcgccg
     8461 ttatcagcga gatgtctact aatgagcgaa aaacatccag ggcctttagt ggtcgaagga
     8521 aaactgacag acgccgagcg catgaagctt gaaagcaact acctgcgcgg caccattgcg
     8581 gaagatttaa acgacggtct gaccggcggc tttaagggcg ataacttcct gctgatccgc
     8641 ttccacggca tgtatcagca ggatgaccgc gacatccgcg ccgaacgtgc tgaacagaag
     8701 ctggagccgc gccacgcgat gctgttgcgc tgccgtctgc cgggtggagt tatcaccacc
     8761 aaacagtggc aggcgatcga taaatttgcc ggtgaaaaca ccatctatgg cagcattcgc
     8821 ctgaccaacc gccagacgtt tcagttccac ggcattctga aaaagaacgt caaaccggtg
     8881 caccagatgc tgcactcggt cggtcttgat gcgctggcga ctgctaacga catgaaccgt
     8941 aacgtactct gcacctcgaa cccttacgag tcgcagctgc acgcagaagc gtacgagtgg
     9001 gcgaagaaga tttctgaaca tctgctgccg cgtacccgcg cgtatgcgga gatctggctc
     9061 gatcaggaaa aagtcgccac caccgatgaa gaaccgatcc ttggtcagac ttacctgccg
     9121 cgtaagttca aaaccacggt agtgatcccg ccgcagaacg atattgatct gcacgccaac
     9181 gacatgaact tcgtggcgat cgcggaaaac ggcaagctgg tgggcttcaa cctgctggtg
     9241 ggcggtgggc tttctatcga acacggtaac aagaaaacct acgcccgcac ggcgagtgag
     9301 tttggctatc tgccgctaga gcatacgcta gcggtggcgg aagccgtcgt gacaactcag
     9361 cgtgactggg gtaaccgaac cgatcgtaaa aatgccaaaa ccaaatacac actggagcgc
     9421 gtgggggttg agacgtttaa agcggaagtg gagcgtcgcg cggggatcaa atttgaaccg
     9481 atccgcccgt atgaatttac aggacgcggc gatcgtattg gctgggttaa gggcattgat
     9541 gataactggc acctgacgct gtttatcgaa aatggtcgca tccttgatta tccggggcgt
     9601 ccgctgaaaa ctggcctgct ggagatcgcg aagatccaca aaggtgattt ccgcattacg
     9661 gcgaaccaga atctgatcat cgccggtgtg ccggaaagcg agaaagcgaa gatcgagaaa
     9721 attgccaaag agagcgggtt aatgaatgcc gtcacgccgc agcgtgaaaa ctcgatggcc
     9781 tgcgtgtcat tcccgacttg cccgctggcg atggcggaag cggagcgttt cctgccgtct
     9841 tttatcgaca acatcgataa tttaatggtg aaacatggtg tcagcgatga gcatatcgtg
     9901 atgcgtgtaa caggctgccc gaacggttgt ggtcgcgcga tgctggcgga agtgggcctg
     9961 gtgggtaaag caccgggtcg ctacaacctg catcttggcg gcaaccgcat tgggacacgt
    10021 atcccacgga tgtataaaga aaacatcacc gagccggaaa tcctggcgtc gcttgatgaa
    10081 ctgatagggc gctgggcgaa agagcgcgaa gcgggtgaag gcttcggcga ctttacggtg
    10141 cgtgcgggca tcattcgccc ggtgctcgat ccggcgcgcg atttgtggga ttaaccatca
    10201 gcccggtctt gtaggcctga taagacgcgt gagcgtcgca tcaggcaagg caaacagtga
    10261 ggaatctatg tccaaactcg atctaaacgc cctgaacgaa ctgccgaagg tagatcgcat
    10321 tctggcgctg gcagaaacta acgccgaact ggaaaaactg gacgctgaag gccgcgttgc
    10381 ctgggcgctg gataatctgc ccggtgaata tgtactttct tccagctttg gcattcaggc
    10441 ggcggtgagc ctgcatctgg tgaatcagat tcacccggat attccggtga tcctcaccga
    10501 caccggctat ctgttcccgg aaacctaccg ctttattgac gagttaacgg acaaactcaa
    10561 gctcaacctg aaagtgtacc gcgctaccga aagcgccgcc tggcaggaag cacgctacgg
    10621 caaactgtgg gaacagggcg ttgaaggcat tgaaaagtac aatgacatca acaaagtcga
    10681 accgatgaac cgggcgctga aagaactgaa tgcgcaaacg tggtttgctg gcctgcggcg
    10741 tgaacaatcc ggcagccgtg ccaatttacc ggtgctggca attcagcgtg gcgtatttaa
    10801 agtgctgccg attatcgact gggataaccg aactatttat cagtacctgc aaaaacatgg
    10861 cctgaaatat cacccattat gggacgaagg atatttatcg gtgggcgata cccatacaac
    10921 ccgtaaatgg gaacccggca tggcggaaga agaaacgcgt ttctttggct taaaaaggga
    10981 atgtggatta cacgaagggt aaatattgcc ggataaatta aacgcctctg tcagaaatga
    11041 tgggggcgtt tttattttgc gagcagcatt ccggcattta aaggtgaaca tcgcagggaa
    11101 aattaacagg tatagaatgg cgttgggtgc ttgaggctgt ctggcgttaa gcattcgcga
    11161 ggttccagat ggacaaaagc cccaggcgat atttctatca acctgaggct cgcgttcgaa
    11221 cgtagcaatt cggatgttag tctcttcttt gcagggaggc aagacatgct gacaaaatat
    11281 gcccttgtgg caatcatcgt actgtgttgt acagtactgg gattcacgtt gatggtaggt
    11341 gactcgttat gtgagttgag tatcagagaa cgtggtatgg agtttaaggc agttctcgct
    11401 tacgaatcga agaagtagcc accacgcggg ggtaaaaccc cgcgtactga attgtgatgg
    11461 tttggtctcc tgcacccttt ttttaaattt atatttttta tgtgaatata tagtctctat
    11521 ttattattat gaatttcaaa tgaaaatcat taatttattc ataatgtaaa tgttttattg
    11581 gctgatatat atcatagata gtttttaaaa tatgcgtaaa tatcctttaa gtttactgaa
    11641 ggataaaaat attgtgactt tctttgattt ctggggaaaa aaccgacgtg gcgagaaaga
    11701 gggtatcgac gactatcacc ttctttgctg gcatttgctg gatgtggccg caatgggcta
    11761 tttaatggtt aaaagaaatt gcttcgggct ggctgattat tttcgtcaat tagggatttc
    11821 tgacaaggaa caggcagctc gatttttcgc ctggttgctg tgctggcacg atattggaaa
    11881 atttgcccgc tcttttcaac aactttacct gccccctgaa ttcaagattc aggaaggtgc
    11941 cagaaaaaat tacgaaaaaa tctctcattc aacgctgggc tactggttgt ggaatcatta
    12001 tttaagtgaa tgtcaggagt tacttccttc atcttcgctc tctccgcgca aacttaggcg
    12061 agtgatagaa atgtggatgc ccgtgacgac agggcatcat ggtcggcccc ctgatcgtat
    12121 ggatgaattg gataattttc tgcctgaaga caaagctgca gcacgagatt ttctccttga
    12181 aatcaagcca ctgtttccgc gaatagagat ccccacattc tgggatgatg acgagggcgt
    12241 tgaacttata aaacaacttt cctggtatat ctctgcaacc gtcgtacttg cagactggac
    12301 agggtcgtca acgcgatttt ttccacgcgt cgcacaggca atggatattg aagattactg
    12361 gcaaaaggct ttagttcagg ctcaaaacgc attaaccgtc tttccgccac aagcagaaac
    12421 cgcgcctttc accggaatta atacgctgtt tccttttatt gagaatacga caccgttaca
    12481 gcaaaaggtg ctggatctgg atatcaatca gccaggacca cagttgttta ttctggaaga
    12541 tgtgaccggc gcaggtaaaa cagaagcggc gcttatcctg gcgcacaggt tgatggctgc
    12601 gggtaacgct caggggctgt tttttggcct gccaacaatg gcaacggcca atgccatgta
    12661 cgatcgactg gtcaaaacct ggcttgcttt ctattcgcca gaggtgttcc ccgctccagc
    12721 ggagataaac cggcgcaata cggtgcggtt ttcccggaag taaaccggac gcactggatg
    12781 cgatgatggg tatcacttag aattccccgc ccctgcggta gaactcccag ctcccatttt
    12841 caaacccatc aagacgcctt cgccagctcc ttcaccaacg gtagcattat ccgcataacg
    12901 tcacggcagc gacgttctat tcttccagga agtgccttat caatatgttg ttgattatcc
    12961 agtcttacgt catgccagct atttcccgcc gggaaggcag gtgtttttgc gcgttgctga
    13021 taaccatcct tatttccaag attccagtta gtcgcttcca ccgaaagtac agcaatgccc
    13081 gctttgtcga atatttctgc gtcattacaa cccccagtgc ctttcggata atttttattc
    13141 aaacccggat tggtcgttgc ggctattccg tgactgcgtg caattgccag cgccctgtcg
    13201 cgcgttaatt tccttactgc ttcaggggtt tttacaccgc tgttgaaata caatttatcg
    13261 ccaacaatta agttatcgag attaatcacc agcagcgtat ttttcttttc ggtgtcactc
    13321 atccgcttga gtaaattctc agcgcctaat tttccttcct cttcgccgct ggtcgccaca
    13381 aatcgaatac catactcggt aggcgtattt ttcaggcgtt ctgccagttc cagcatgaca
    13441 cctaaacctg cggcgttatc atctattcct tgtaacgtca gtccgccgag attggcatct
    13501 gcgtcagcat cgctcagcgg ggcgtaagta tccagatgcg ccataatgat gatctgctgc
    13561 ggcgctttgc cttcatgagc ggcaatcacc gtacttcctg tcacgttatg ccagttcttg
    13621 cgattatcgc gggcggtata aatataccga ctattaaatg tccgaatatc actgcgataa
    13681 cccatttgct gaaactgttg gcgaatataa tcggcagata acatttctgc tggagttccg
    13741 gtcatgcgtc ccggaaagaa agtagcaata tgtcgtgcct gggtattagc aaaatcgcca
    13801 ggtttaggtg acgaggcgtg tacggggaga ataaagcata cgccgagcgc cagggcagcg
    13861 gtacggtggc gcaatgcgga aaacatagtg agtccttaaa taccatgcaa atttttttac
    13921 cgccatagta tgaaactgcc gctgcgctaa aacaatttca aatcttccta aacgcccgaa
    13981 atccggtgcc ttaagcactt tttgatatta gctttgccaa atcgttattc cgttaaggaa
    14041 ctactcattc taattggtaa tttcattcgt tctcttacgc tccctatagt cgaaacatct
    14101 gatggcaaga aaatagcggt attgcaaagg aacggttatg gctcaaaaac gacttactca
    14161 cctgcggcaa ctggaggcgg aaagcatcca cattattcgc gaggtggcgg cagaattctc
    14221 aaatccggtg atgctctact ctatcggtaa agattccagc gtcatgctgc atctggcgcg
    14281 caaggcgttt tatccaggta cgctgccttt cccgttgctg catgtcgata ccggctggaa
    14341 attccgcgag atgtatgagt tccgcgatcg tacggcgaaa gcctacggct gcgagctgct
    14401 ggtgcataaa aacccggaag gcgtggcgat ggggattaat ccattcgtgc acggcagcgc
    14461 gaaacatacc gatattatga aaactgaagg cctgaaacag gcgctgaaca aatacggttt
    14521 tgatgccgcc ttcggtggtg cgcgccgtga cgaagagaaa tcccgcgcta aagagcgaat
    14581 ctactctttc cgtgaccgct tccatcgctg ggatccgaaa aatcagcgcc cggagctgtg
    14641 gcacaactac aacgggcaaa ttaacaaagg cgaaagcatc cgcgtcttcc cgctctctaa
    14701 ctggaccgag caggatatct ggcaatacat ctggctggaa aatatcgaca ttgtgccgct
    14761 gtatctcgct gcggaacgtc cggttctgga acgcgacggt atgttgatga tgattgatga
    14821 caaccgtatc gacctgcaac cgggcgaagt gatcaaaaaa cggatggtgc gtttccgtac
    14881 gctgggctgc tggccgctga ccggtgcggt ggagtcaaat gcacaaacac tgccggaaat
    14941 catcgaagag atgctggttt ccaccaccag tgaacgtcag ggtcgcgtga ttgaccgcga
    15001 ccaggcggga tccatggagc tgaaaaaacg tcaggggtat ttttaagatg aacaccgtac
    15061 ttgcacaaca aatcgccaat gaaggcggcg tcgaagcctg gatgattgcg caacaacata
    15121 aaagcctgct gcgttttctg acctgtggta gcgtcgatga cggcaaaagt accctgattg
    15181 gtcgtctgct gcacgatacc cgccaaatct acgaagatca gctctcatcg ctgcataacg
    15241 acagtaagcg tcacggaacc cagggcgaaa agctggatct ggctctgctg gtggacggcc
    15301 tgcaagctga gcgcgaacag ggcatcacta ttgatgtggc ctaccgctat ttctctaccg
    15361 agaagcgtaa atttattatc gccgacaccc cagggcacga gcagtacact cgcaatatgg
    15421 cgactggcgc atcgacatgt gaactggcga tcttactgat tgatgcccgt aaaggcgtgc
    15481 tcgatcaaac ccgtcgtcac agttttatct ccacactgtt ggggatcaaa catctggtcg
    15541 tggcgatcaa caaaatggat ctggtggatt acagtgaaga gacgttcacc cgtattcgtg
    15601 aagattatct gacctttgcc gggcagctgc cgggtaatct ggatatccgc tttgtgccgc
    15661 tctccgcact ggaaggtgac aacgtggctt cgcaaagtga aagtatgccg tggtacagcg
    15721 gtctgacact gctcgaagtg ctggaaaccg tggagatcca gcgagtggtg gatgcccagc
    15781 caatgcgctt cccggtgcag tacgttaacc gcccaaatct cgattttcgt ggctacgccg
    15841 gaatgctggc atccggtcgc gtggaagtcg ggcaacgtgt aaaagtgctg ccctctggtg
    15901 tggaatcaaa cgtcgcgcgg atcgtgactt ttgatggtga tcgcgaagaa gtctttgccg
    15961 gagaagcgat caccctggtg ctgacggatg agatcgatat cagccgtggt gatctgctgc
    16021 tggcggcaga cgaagcgtta ccggcggtgc agagtgcgtc ggtggatgtg gtatggatgg
    16081 cggaacagcc gctttccccg ggccagagtt atgacatcaa aattgccggt aagaagacgc
    16141 gtgctcgtgt tgatggcatt cgttatcagg ttgatattaa taaccttacc caacgcgaag
    16201 ttgaaaacct gccgctgaac ggcatcggcc tggtggatct cacttttgac gagccactgg
    16261 tgttagatcg ctatcagcaa aatccggtta ccggtgggct gatttttatc gatcgcctga
    16321 gcaatgtgac cgtgggtgcc ggtatggtgc acgagccagt tagccaggca acagctgcgt
    16381 catctgaatt cagtgcattc gaactggaat tgaatgccct ggttcgtcgc cactttccgc
    16441 actggggcgc gcgcgatttg ctgggggata aataatggcg ctgcatgacg aaaacgtcgt
    16501 ctggcatagt catccggtca ctgtgcaaca acgcgaacta caccacggtc atcgtggtgt
    16561 agtgctgtgg tttaccggcc tctccgggtc cggtaaatca acggtcgccg gggcgctgga
    16621 ggaggcgtta cataaactca gcgtcagtac gtatctgctg gatggcgaca atgttcgcca
    16681 cggattatgc agcgatctcg gttttagcga tgccgatcgt aaagagaata tccgtcgcgt
    16741 cggtgaagtg gcgaatttga tggttgaagc cggactggtg gtgctgaccg catttatctc
    16801 gccacaccgc gccgaacgcc agatggttcg cgaacgcgta ggagaagggc gctttattga
    16861 agtgtttgtc gatacgccgc tggcgatttg cgaagcccgc gatccaaaag gcttatataa
    16921 gaaagcgcgt gccggtgaac tgcgcaactt tacgggaata gattccgttt acgaagcgcc
    16981 tgaatcggca gaaattcatc tcaatggtga acaattagta acaaatttgg tacagcaatt
    17041 attagatctg ttgagacaga acgatattat cagatcctga gacaccaccg ggtttccatg
    17101 cccggttcgt caaagtaaca ggatcaaata tgcgtaatag ccataacatt acactaacaa
    17161 ataacgacag ccttactgag gatgaagaaa ctacatggtc actgcctggt gccgtggtcg
    17221 gttttatctc ctggttattt gcgctggcga tgccgatgtt gatttatggc tctaacacgc
    17281 tgttcttctt tctctacacc tggcctttct ttctggcact gatgcctgtc gcggtagtgg
    17341 tggggattgc gctgcattca ttgatggacg gaaagctacg ctacagtatt gttttcaccc
    17401 tggtgacagt aggcattatg tttggcgcac tgtttatgtg gctactgggc taatttgtac
    17461 tttcttgtct cctctgttca taattcaaac cgtaactaat aatgagatta tgttctgcac
    17521 gccctgggta tacgtaacaa tgggcaaatg tggtacattt gcccgcgttg tcgcggtatc
    17581 cccaacagag gatgtagagt cgtcttcgga tgcatgggat gatgatgcag tttttcaggg
    17641 ggcaggatgg gtaaactaac gctgctgttg ctggctattc tggtctggct acagtattcg
    17701 ctgtggttcg gtaagaacgg tatacatgac tatacccgcg tcaatgatga tgtggcggca
    17761 ctgcaagcta caaacgcgaa acttaaagcg cgaaacgatc aactttttgc cgaaattgac
    17821 gatctcaatg gcggccagga ggcgctcgaa gagcgtgcgc gtaatgaact cagcatgacc
    17881 aggccgggcg aaacttttta tcgtctggtg cctgacgcgt cgaagcgcgc acagtctgcg
    17941 gggcaaaaca atcgataaat cagcccggga attaacatgg caaccactca tttggatgtt
    18001 tgcgccgtgg ttccggcggc cggatttggc cgtcgaatgc aaacggaatg tcctaagcaa
    18061 tatctctcaa tcggtaatca aaccattctt gaacactcgg tgcatgcgct gctggcgcat
    18121 ccccgggtga cacgtgtcgt cattgccata agtcctggcg atagccgttt tgcacaactt
    18181 cctctggcga atcatccgca aatcaccgtt gtagatggcg gtgatgagcg tgccgattcc
    18241 gtgctggcag gtctgaaagc cgctggcgat gtgcagtggg tattggtgca tgacgccgct
    18301 cgtccttgtt tgcatcagga tgaccttgcg cgattgttgg cgttgagcga aaccagccgc
    18361 acgggaggga tcctcgccgc accagtgcgc gatactatga aacgtgccga accgggcaaa
    18421 aatgccattg ctcataccgt tgatcgcaac ggcttatggc acgcgctgac gccgcaattt
    18481 ttccctcgtg agctgttaca tgactgtctg acgcgcgctc taaatgaagg cgctaccatt
    18541 accgacgaag cctcggcgct ggaatattgc ggatttcatc cgcagttggt cgaaggccgc
    18601 gcggataaca ttaaagtcac gcgcccggaa gatttggcac tggccgagtt ttacctcacc
    18661 cgaaccatcc atcaggagaa tacataatgc gaattggaca cggttttgac gtacacgcct
    18721 ttggcggtga aggcccaatt atcattggtg gcgtacgcat tccttacgaa aaaggattgc
    18781 tggcgcattc tgatggcgac gtggcgcttc atgcgttgac cgatgcattg cttggcgcgg
    18841 cggcgctggg ggatatcggc aaactgttcc cggataccga tccggcattt aaaggtgccg
    18901 acagccgcga gctgctacgc gaagcctggc gtcgtattca ggcgaagggt tatacccttg
    18961 gcaacgtcga tgtcactatc atcgctcagg caccgaagat gttgccgcac attccacaaa
    19021 tgcgcgtgtt tattgccgaa gatctcggct gccatatgga tgatgttaac gtgaaagcca
    19081 ctactacgga aaaacttgga tttaccggac gtggggaagg gattgcctgt gaagcggtgg
    19141 cgctactcat taaggcaaca aaatgattga gtttgataat ctcacttacc tccacggtaa
    19201 accgcaaggc accgggctgc tgaaagccaa tccggaagac tttgtggtgg tggaagattt
    19261 gggctttgag cctgatggtg aaggtgagca tattctggtt agaatcctca aaaacggctg
    19321 caatacccgt tttgtggcgg atgcactggc gaaattcctg aaaattcatt cccgtgaagt
    19381 cagcttcgct gggcaaaaag acaaacatgc tgtaacggaa cagtggttat gtgctcgcgt
    19441 gccgggcaag gaaatgcccg atctgagcgc ctttcaactg gaaggctgcc aggtgctgga
    19501 gtatgcgcgg cacaagcgca agctgcgttt aggggcgctg aaaggtaacg cctttaccct
    19561 ggttctgcgc gaagtgagca atcgcgatga cgttgaacaa cgtctgatcg atatttgcgt
    19621 aaaaggtgta ccgaactact tcggtgccca acgttttggg attggcggta gcaacttgca
    19681 gggcgcgctg cgctgggcgc aaaccaatac tccggtgcgc gatcgcaata aacggagttt
    19741 ttggttgtcg gcagcccgca gtgcgttgtt taatcagatt gttgctgagc gcctcaaaaa
    19801 agcagacgtt aatcaagttg ttgacggcga tgcgctacaa ttagccggac gtggtagctg
    19861 gtttgtagca accaccgaag aactggcgga attacagcgt cgcgtcaacg ataaagagct
    19921 gatgataacc gccgcattgc cgggcagtgg cgaatgggga actcagcgtg aagcgctggc
    19981 attcgaacaa gcagctgtcg ccgcagaaac tgaattacaa gctttactgg tgcgcgaaaa
    20041 agttgaagcc gcgcgcagag cgatgctgct gtatccgcaa caattaagct ggaactggtg
    20101 ggatgacgtc accgtagaga tccgtttctg gcttccggcg ggtagttttg caaccagcgt
    20161 tgtcagggaa cttatcaaca caacaggtga ttatgcgcat attgctgagt aattatgacg
    20221 gggtacatgc acccggtata caaacgctgg cgaaagcctt gcgtgagttt gctgacgttc
    20281 aggtggtcgc ccccgatcgt aaccgcagcg gcgcttcaaa ttctctgaca ctggaatcct
    20341 ccctgcgcac gtttaccttt gaaaatggtg atattgctgt gcaaatggga accccgaccg
    20401 attgtgtcta tcttggcgtg aatgctctga tgcgtccgcg cccggacatt gttgtgtccg
    20461 gaattaacgc cgggccgaat ctgggggatg atgttattta ttccggtacg gtagccgccg
    20521 cgatggaagg ccgtcattta ggttttccgg cgctggccgt ctcgcttgac gggcataaac
    20581 attacgacac tgccgcggcg gtaacctgtt caattttgcg cgcactgtgt aaagagccgc
    20641 tgcgcaccgg acgtattctt aatattaacg ttcaggattt acccttggat caaatcaaag
    20701 gtattcgcgt gacgcgctgc ggtacacgac atccggcaga tcaggtgatc ccgcagcaag
    20761 atccgcgcgg caatacgctg tactggattg gcccgccggg cggtaaatgt gatgccggtc
    20821 cggggaccga ttttgctgcg gtagatgagg gctatgtctc catcacgccg ctgcatgtgg
    20881 atttaactgc gcatagcgcg caagatgtgg tttcagactg gttaaacagc gtgggagttg
    20941 gcacgcaatg gtaagcagac gcgtacaagc acttctggat caattacgtg cgcaaggtat
    21001 tcaggatgag caggtgctga atgcacttgc cgccgtgccg cgtgaaaaat tcgttgatga
    21061 agcgtttgaa caaaaagcct gggacaatat cgccttgccg ataggtcagg ggcagacaat
    21121 ttcgcagccg tatatggtgg cgcgaatgac cgaattactc gagctgacgc cgcagtcgcg
    21181 ggtgctggaa attggcactg gttcgggata tcaaacggca atcctggcgc atcttgtcca
    21241 gcatgtttgc tcggttgaac ggattaaagg cttgcagtgg caggcacgtc gccgcctgaa
    21301 aaatcttgat ttacataatg tttcaacccg tcatggtgat ggatggcaag gttggcaggc
    21361 acgtgcgccg tttgacgcta tcattgttac ggcggcaccg ccggaaattc caactgcgct
    21421 aatgacgcag ctggacgaag gcgggattct cgtcttaccc gtaggggagg agcaccagta
    21481 tttgaaacgg gtgcgtcgtc ggggaggcga atttattatc gataccgtgg aggccgtgcg
    21541 ctttgtccct ttagtgaagg gtgagctggc ttaaaacgtg aggaaatacc tggatttttc
    21601 ctggttattt tgccgtaggt cagcgtatcg tgaacatctt ttccagtgtt cagtagggtg
    21661 ccttgcacgg taattatgtc actggttatt aaccaatttt tcctggggga taaatgagcg
    21721 cgggaagccc aaaattcacc gttcgccgca ttgcggcttt gtcactggtt tcgctatggc
    21781 tggcaggctg ttctgacact tcaaatccac cggcaccggt cagctccgtt aatggcaatg
    21841 cgcctgcaaa tactaattct ggtatgttga ttacgccgcc gccgaaaatg gggacgacgt
    21901 ctacagcgca gcaaccgcaa attcagctgg tacagcagcc acaaattcag gctactcaac
    21961 aaccgcaaat ccagccagtg cagccagtag ctcagcagcc ggtacagatg gaaaacggac
    22021 gcatcgtcta taaccgtcag tatgggaaca ttccgaaagg cagttatagc ggcagtacct
    22081 ataccgtgaa aaaaggcgac acacttttct atatcgcctg gattactggc aacgatttcc
    22141 gtgaccttgc tcagcgcaac aatattcagg caccatacgc gctgaacgtt ggtcagacct
    22201 tgcaggtggg taatgcttcc ggtacgccaa tcactggcgg aaatgccatt acccaggccg
    22261 acgcagcaga gcaaggagtt gtgatcaagc ctgcacaaaa ttccaccgtt gctgttgcgt
    22321 cgcaaccgac aattacgtat tctgagtctt cgggtgaaca gagtgctaac aaaatgttgc
    22381 cgaacaacaa gccaactgcg accacggtca cagcgcctgt aacggtacca acagcaagca
    22441 caaccgagcc gactgtcagc agtacatcaa ccagtacgcc tatctccacc tggcgctggc
    22501 cgactgaggg caaagtgatc gaaacctttg gcgcttctga ggggggcaac aaggggattg
    22561 atatcgcagg cagcaaagga caggcaatta tcgcgaccgc agatggccgc gttgtttatg
    22621 ctggtaacgc gctgcgcggc tacggtaatc tgattatcat caaacataat gatgattacc
    22681 tgagtgccta cgcccataac gacacaatgc tggtccggga acaacaagaa gtgaaggcgg
    22741 ggcaaaaaat agcaaccatg ggtagcaccg gaaccagttc aacacgcttg cattttgaaa
    22801 ttcgttacaa ggggaaatcc gtaaacccgc tgcgttattt gccgcagcga taaatcggcg
    22861 gaaccaggct tttgcttgaa tgttccgtca agggatcacg ggtaggagcc accttatgag
    22921 tcagaatacg ctgaaagttc atgatttaaa tgaagatgcg gaatttgatg agaacggagt
    22981 tgaggttttt gacgaaaagg ccttagtaga agaggaaccc agtgataacg atttggccga
    23041 agaggaactg ttatcgcagg gagccacaca gcgtgtgttg gacgcgactc agctttacct
    23101 tggtgagatt ggttattcac cactgttaac ggccgaagaa gaagtttatt ttgcgcgtcg
    23161 cgcactgcgt ggagatgtcg cctctcgccg ccggatgatc gagagtaact tgcgtctggt
    23221 ggtaaaaatt gcccgccgtt atggcaatcg tggtctggcg ttgctggacc ttatcgaaga
    23281 gggcaacttg gggctgatcc gcgcggtaga gaagtttgac ccggaacgtg gtttccgctt
    23341 ctcaacatac gcaacctggt ggattcgcca gacgattgaa cgggcgatta tgaaccaaac
    23401 ccgtactatt cgtttgccga ttcacatcgt aaaggagctg aacgtttacc tgcgaaccgc
    23461 acgtgagttg tcccataagc tggaccatga accaagtgcg gaagagatcg cagagcaact
    23521 ggataagcca gttgatgacg tcagccgtat gcttcgtctt aacgagcgca ttacctcggt
    23581 agacaccccg ctgggtggtg attccgaaaa agcgttgctg gacatcctgg ccgatgaaaa
    23641 agagaacggt ccggaagata ccacgcaaga tgacgatatg aagcagagca tcgtcaaatg
    23701 gctgttcgag ctgaacgcca aacagcgtga agtactggca cgtcgattcg gtttgctggg
    23761 gtatgaagcg gcaacactgg aagatgtagg tcgtgaaatt ggcctcaccc gtgaacgtgt
    23821 tcgccagatt caggttgaag gcctgcgccg tttgcgcgaa atcctgcaaa cgcaggggct
    23881 gaatatcgaa gcgctgttcc gcaagtaagt aagcatctgt cagaaaggcc agtctcaagc
    23941 gaggctggcc tttttctttt gtttgggaag cttgtgtttt tgtcgcctgg ataagacacg
    24001 tcagcgtcct acccggcaat cagcattcta tgtgctcatc atcttgcgca ccaactgcat
    24061 aaacagctcc tgctcttctc cactcaaacg ccccaaaaat tcctcatcca cgctgtcacc
    24121 aataggtatc gccgcagcaa gtagcttttc tccttcggca gtcagccaga caaagcgtcg
    24181 ccgcttgtca gcgacgtcat gttcacgtct gactaagcca cgattttcca ttcttgccaa
    24241 catttctgct agcgttgctt tggtgctgac tgctgcttct atcagtgcca cctgttcaat
    24301 accaggttta tcagcaatgg cgcgcatcac cgcgtactgt ggtttggtca ggtcaggtaa
    24361 ctcgtgctgc cagcgagccg tgtgctgctg aaaaagctgt cgtaactgat ggaacgcttt
    24421 atttcgtaac gccatgtaaa ctccgggcta attctctgtc gctatcatag cggtttttta
    24481 gtgacgaaga agatgttgtt acttttcaat ccttgccgct gtggaaatga aggagtatgt
    24541 taataataac gttcgtatac gaataattaa gagaataagc aatgaaactg atcgtcggga
    24601 tgacaggggc taccggtgcg cctcttggtg tggcattact gcaagcgctg cgtgagatgc
    24661 cgaatgtcga gactcatctg gtgatgtcga agtgggcgaa aaccaccatt gaactggaaa
    24721 cgccttacag cgctcgcgat gttgctgccc tcgcagactt cagccataac ccggcggatc
    24781 aggcggcgat catctcatcc ggttcttttc gtaccgacgg catgatcgtt attccgtgca
    24841 gtatgaaaac gctcgccggt atccgcgctg gttacgccga tggcctggta gggcgcgcgg
    24901 cggacgtcgt gctcaaagaa ggccgcaaac tggtgctggt gccgcgtgaa atgccgctta
    24961 gcaccatcca tctcgaaaat atgctcgcac tttcacgcat gggcgtggcg atggtgccgc
    25021 cgatgcctgc cttttataac catcccgaaa cggtagatga cattgtccac catgtggtag
    25081 cccgcgtgct ggatcaattt ggcctcgaac atccccacgc caggcgctgg caaggattgc
    25141 cgcaggcccg gaatttttct caggagaatg aataatggca tttgatgatt tacgcagctt
    25201 tttacaggcg cttgatgacc acggccagtt actgaaaatc agcgaagaag tgaacgctga
    25261 gccggatctg gcagcagcag ctaacgccac cgggcgtatc ggcgacggcg cgcccgcgct
    25321 gtggtttgat aatattcgcg gctttaccga tgcccgcgtg gcgatgaata ccatcggttc
    25381 ctggcagaac cacgcgattt cccttggcct gccaccaaac accccggtca aaaagcagat
    25441 tgatgagttt atccgccgct gggataactt cccgattgcc ccggagcgcc gcgccaatcc
    25501 agcctgggcg cagaacaccg tcgatggcga agagatcaac ctgttcgata ttctgccgct
    25561 gtttcgcctg aatgatggcg atggcggttt ctatctcgac aaagcgtgcg tggtttcccg
    25621 cgatccgctc gacccggata acttcggcaa gcagaacgtc ggcatctacc gcatggaagt
    25681 gaagggcaag cgtaagctcg gcctgcaacc ggtgccgatg cacgatatcg ccctgcatct
    25741 gcataaagca gaagagcgcg gtgaagatct gccgattgcg atcacgctcg gtaacgatcc
    25801 gatcatcacg ctgatggggg ccacgccgct gaaatacgat cagtctgagt atgaaatggc
    25861 aggcgcgctg cgtgaaagcc cgtacccgat cgccaccgct ccgctgaccg gttttgatgt
    25921 gccgtggggt tcagaagtga tcctcgaagg ggttatcgaa agccgtaaac gcgaaatcga
    25981 agggccgttc ggtgagttta ccgggcacta ctccggtggg cgcaacatga ccgtggtgcg
    26041 catcgataaa gtctcttatc gcagcaaacc gattttcgaa tcactctatc tcggtatgcc
    26101 gtggaccgaa atcgactacc tatgactagg taatttattc aagataaccg ggaagagatt
    26161 aaaaaagacg ggaatagatt ggaatcaaca agttaggaac tcaaggtttt caagataaag
    26221 cgagtccaaa aatgttaaac ggttcgatat caataaaaca ttcaagataa ctcacaggtt
    26281 cttacaatag cctttaaaga ccgttcaaaa acgattcagg tgtttattat agcgctgatg
    26341 ggtgcaagcg ccaaaaccaa tgtaatttta gcacagctag gcacttgcac tgggttatgc
    26401 gctggctgct ttctgaggta gatttaagtg atcgccctta gcttcggaca acgcgtaaat
    26461 ctctctaacc ttttcgtctg gcaaattacc aaggcttaac gctatggctc tacgaactcc
    26521 aactagagcg tgcaactctt ggatgtgctc atccagaaga atgagtaatt gctccgctcc
    26581 ttttctccca agaacatcag ctacttgttg tttttcttgt ggtttaagaa atgacaagaa
    26641 cattagaatt tgagcatcaa cattatccgc attctctttt ttgggttctt tggaatcttt
    26701 tagttcaaca tcaccctctc ccgtcatgag ccattccaag gtaacatttt cgctttctgc
    26761 tatctttttt aactgatacg ggcgtggtat cggtgcgtaa tcccgcctgt tgtaataatt
    26821 ttttaaagtg ccttcattga tcccccatgc ccgagcagcc gcatttcttg aagggtatcg
    26881 ttctactaac ctaaagattc tttcgataat cggttctttt ccatcggcag gaaaagaacc
    26941 ttggggtgct tttctcattc ttggttcctt tctatctaat tcattgataa ataataaaat
    27001 taaaaaataa accataaaaa caccaaagaa ccctttgcag ggaatctttg gtatcttaat
    27061 ctttatccat acggatatcc gaccggatat ccgcgcgggt aattttgtag gttaaaggaa
    27121 agatgagaca aagagaagat agtgaccgcg attggccttc gctgaagatt cagctggcgc
    27181 tcaaagataa cgggtggtct tccttccggg agctttcact ccatgctggt tatgctcagg
    27241 attcactgaa agcggtagtt cgcacaccaa acaaaaaata cgaacgcgta atttctgacg
    27301 ctataggtgt accgccagaa atcatctggc ccagccggta tcgtaacgat ggatttatca
    27361 ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg acctgctcgg cgtaccagga
    27421 atgccagcca ccacaaaagg catccgacag gcgttacagc gtttttctcg tgatcttggt
    27481 gacgttagtc gccgtcggga aggcacaaaa gccattgagt accacattga ttgtctgcct
    27541 gaaataacac gaaaggcact gcgtgaacgc tatgtggaac agctggtggc tacagagaac
    27601 aatgtttctg aagtgaaagc tgttaccaga aaaacacgca atcctgacgc tgtccaggca
    27661 atcgaagcat atcgcggttc tccacaactg atggaagaac gcctgaatgc gctgaccgaa
    27721 aaccagcgct gggtatctga agcaagagct gcgctggtgg tggaagtgct gaagctggaa
    27781 agcgccggta accccgggcg actgaaagcc attaactttc ttgttgaaaa agcccgtaaa
    27841 ggtgagctgc cggagcgcct gcaacaggcc gcagttaacg ccaatgcaaa acgtggcgct
    27901 aatcgtacta tcagccgcga tccactttac cagtgggttc tgaaatacaa ccagtcgcag
    27961 aacgccgccg aacggctgct gttactggca ccgggaaaac gcgacgaaat aaaaccggaa
    28021 gaaatcagct ggctgcctga atttctggcg cagtatcgcc aggtgaacgg cagacccatg
    28081 tcagaagcct acgaggattt tgtcgcagaa tggcagcgtc gccatgcgga cgaaccgtac
    28141 atgctggagg tgatgccttc ctatgatgtt gtccggtatg ccatgaaaaa actgccggaa
    28201 gtggtgaaac aaaaagggcg cgttaccggc agtgaatacc gtcagcttga aggattcacc
    28261 cgccgcgact ggacagctat gccggtgaat tatgtctgga ttggtgacgg tcacggtatg
    28321 aagctgaaat gtgcgcatcc gatccacggg cgtccattct caccggaagt cacgtttgtg
    28381 atcgatggtg gaacacggtt tgttgttggc tggagtcttg accttgccga aaacgtgttc
    28441 gccgttgccg gtgcgataca gcacggcatt cgcaaccacg gaaaaccgtt cctgtattac
    28501 tcggataacg gttccggtga aacggctgac atgctggaca aagaggtcgt gggtattctg
    28561 ccacgcctgg ggattaaaca ccctaccggt attgccggta atccacaggg acgcggcatt
    28621 attgaacgcc tgaaccggac actgccgatg cgtatagcga ggaggtatcg cacctatttt
    28681 ggcaagggtg ctgaccggga atcgttgcgt gtacttaacc gtgatctgcg ttcagcgttt
    28741 aacgccctgc aacaggacaa gcctctgaac gaccgccaga aagcggcaat gcgcgagctg
    28801 ccatcgtggg cggagcttat tgaggctatt cgtgaaggcg tggaatggta caacaaccgc
    28861 ccgcattctg agctgccgat gaaaccgaac ggacggcatt acagcccgac agagttcaga
    28921 aaaaaacgcc aggcagaaga agacaccgaa attgagtggc tgagtgatct tgagctgcgc
    28981 gacatgttcc gcccaatggt ggaacgaccg gtaagacgct gcgaaattca gtggctgaac
    29041 aatatttact acgcgccgga gttgcgtgac gaacacggtc gcaaagtgct tatcagttat
    29101 gacattcatg acgccgagcg aattacggta cgtcgcaagg acggcagctt tatctgtgag
    29161 gcgatatgga acggcaataa acgcgcagcc tttgctgtca gtgctgaata tcacaaacaa
    29221 cagcagcgta ttaaaggaat gcgcaaacgc gctgaggaaa aaattcgtga tgctgaagat
    29281 gaaggtattc agattctgga acacaaacag gctgaaccct ggc
//
LOCUS       DAJTRM010000046        29015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRM010000046 DAJTRM010000000
VERSION     DAJTRM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.046"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO322_002136"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO322_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5907011.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO322_002137"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO322_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5907012.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO322_002138"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO322_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5907013.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO322_002139"
     CDS             4843..5082
                     /locus_tag="OO322_002139"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907014.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO322_002140"
     CDS             5092..5262
                     /locus_tag="OO322_002140"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5907015.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO322_002141"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO322_002141"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5907016.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO322_002142"
     CDS             6724..6990
                     /locus_tag="OO322_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5907017.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO322_002143"
     CDS             7084..7773
                     /locus_tag="OO322_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5907018.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO322_002144"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO322_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5907019.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO322_002145"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO322_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5907020.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO322_002146"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO322_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5907021.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO322_002147"
     CDS             complement(10475..12193)
                     /locus_tag="OO322_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5907022.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO322_002148"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO322_002148"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5907023.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO322_002149"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO322_002149"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5907024.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO322_002150"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO322_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5907025.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO322_002151"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO322_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5907026.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO322_002152"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO322_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO322_002153"
     CDS             18432..18746
                     /locus_tag="OO322_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5907027.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO322_002154"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO322_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5907028.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO322_002155"
     CDS             20570..21862
                     /locus_tag="OO322_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5907029.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO322_002156"
     CDS             complement(22016..23665)
                     /locus_tag="OO322_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5907030.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO322_002157"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO322_002157"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5907031.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO322_002158"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO322_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5907032.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO322_002159"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO322_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5907033.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO322_002160"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO322_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..>29015
                     /locus_tag="OO322_002161"
     CDS             28753..>29015
                     /locus_tag="OO322_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5907034.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQ"
BASE COUNT         7276 a         7771 c         7345 g         6623 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctagatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatg
//
LOCUS       DAJTRM010000047        28985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRM010000047 DAJTRM010000000
VERSION     DAJTRM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.047"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..213)
                     /locus_tag="OO322_002162"
     CDS             complement(<1..213)
                     /locus_tag="OO322_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5907035.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO322_002163"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO322_002163"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5907036.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO322_002164"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO322_002164"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5907037.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO322_002165"
     CDS             2894..3634
                     /locus_tag="OO322_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5907038.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO322_002166"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO322_002166"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5907039.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO322_002167"
     CDS             complement(4192..4794)
                     /locus_tag="OO322_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5907040.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OO322_002168"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OO322_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5907041.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OO322_002169"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OO322_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5907042.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OO322_002170"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OO322_002170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5907043.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO322_002171"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO322_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5907044.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO322_002172"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO322_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5907045.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OO322_002173"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OO322_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5907046.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OO322_002174"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OO322_002174"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5907047.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OO322_002175"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OO322_002175"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5907048.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO322_002176"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO322_002176"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5907049.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO322_002177"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO322_002177"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5907050.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OO322_002178"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OO322_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5907051.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="OO322_002179"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="OO322_002179"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5907052.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="OO322_002180"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="OO322_002180"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5907053.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO322_002181"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO322_002181"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5907054.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="OO322_002182"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="OO322_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /locus_tag="OO322_002183"
     CDS             20653..21009
                     /locus_tag="OO322_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5907055.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="OO322_002184"
     CDS             21336..21995
                     /locus_tag="OO322_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5907056.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="OO322_002185"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="OO322_002185"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5907057.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO322_002186"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO322_002186"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5907058.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="OO322_002187"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="OO322_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO322_002188"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO322_002188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5907059.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="OO322_002189"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="OO322_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5907060.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="OO322_002190"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="OO322_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="OO322_002191"
     CDS             27338..27679
                     /locus_tag="OO322_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5907061.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..28985)
                     /locus_tag="OO322_002192"
                     /pseudo
     CDS             complement(28072..28985)
                     /locus_tag="OO322_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7333 a         7129 c         7470 g         7053 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtat
//
LOCUS       DAJTRM010000048        28912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRM010000048 DAJTRM010000000
VERSION     DAJTRM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.048"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO322_002193"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO322_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5907062.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLKRATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO322_002194"
     CDS             complement(2138..2584)
                     /locus_tag="OO322_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5907063.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO322_002195"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO322_002195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5907064.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO322_002196"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO322_002196"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5907065.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO322_002197"
     CDS             complement(5347..6009)
                     /locus_tag="OO322_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5907066.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO322_002198"
     CDS             6182..6595
                     /locus_tag="OO322_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5907067.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO322_002199"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO322_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5907068.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO322_002200"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO322_002200"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5907069.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO322_002201"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO322_002201"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5907070.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO322_002202"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO322_002202"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5907071.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO322_002203"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO322_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5907072.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO322_002204"
     tRNA            complement(9861..9948)
                     /locus_tag="OO322_002204"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO322_002205"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO322_002205"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5907073.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO322_002206"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO322_002206"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5907074.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO322_002207"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO322_002207"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5907075.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO322_002208"
     CDS             14005..14592
                     /locus_tag="OO322_002208"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5907076.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO322_002209"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO322_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5907077.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO322_002210"
     CDS             14984..15841
                     /locus_tag="OO322_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5907078.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO322_002211"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO322_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5907079.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO322_002212"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO322_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5907080.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO322_002213"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO322_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5907081.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO322_002214"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO322_002214"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5907082.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO322_002215"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO322_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO322_002216"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO322_002216"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5907083.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO322_002217"
     CDS             complement(22884..24023)
                     /locus_tag="OO322_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5907084.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO322_002218"
     CDS             complement(24069..26165)
                     /locus_tag="OO322_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5907085.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO322_002219"
     CDS             complement(26165..26911)
                     /locus_tag="OO322_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5907086.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO322_002220"
     CDS             complement(26908..27552)
                     /locus_tag="OO322_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5907087.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO322_002221"
     CDS             complement(27659..27964)
                     /locus_tag="OO322_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907088.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO322_002222"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO322_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5907089.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7001 a         7319 c         7600 g         6992 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttt gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta at
//
LOCUS       DAJTRM010000049        28607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRM010000049 DAJTRM010000000
VERSION     DAJTRM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.049"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..625
                     /locus_tag="OO322_002223"
                     /pseudo
     CDS             1..625
                     /locus_tag="OO322_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1008
                     /locus_tag="OO322_002224"
     CDS             622..1008
                     /locus_tag="OO322_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907090.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1033..3246
                     /gene="tssI"
                     /locus_tag="OO322_002225"
     CDS             1033..3246
                     /gene="tssI"
                     /locus_tag="OO322_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5907091.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3269..3714
                     /locus_tag="OO322_002226"
                     /pseudo
     CDS             3269..3714
                     /locus_tag="OO322_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3736..4143
                     /locus_tag="OO322_002227"
     CDS             3736..4143
                     /locus_tag="OO322_002227"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5907092.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4140..6428
                     /locus_tag="OO322_002228"
     CDS             4140..6428
                     /locus_tag="OO322_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5907093.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6425..7414
                     /locus_tag="OO322_002229"
     CDS             6425..7414
                     /locus_tag="OO322_002229"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907094.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7514..7717
                     /locus_tag="OO322_002230"
     CDS             7514..7717
                     /locus_tag="OO322_002230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907095.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7735..8055
                     /locus_tag="OO322_002231"
     CDS             7735..8055
                     /locus_tag="OO322_002231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907096.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8121..8353)
                     /locus_tag="OO322_002232"
                     /pseudo
     CDS             complement(8121..8353)
                     /locus_tag="OO322_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8463..9233)
                     /gene="yafV"
                     /locus_tag="OO322_002233"
     CDS             complement(8463..9233)
                     /gene="yafV"
                     /locus_tag="OO322_002233"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5907097.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9414..9860
                     /gene="ivy"
                     /locus_tag="OO322_002234"
     CDS             9414..9860
                     /gene="ivy"
                     /locus_tag="OO322_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5907098.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9903..12347)
                     /gene="fadE"
                     /locus_tag="OO322_002235"
     CDS             complement(9903..12347)
                     /gene="fadE"
                     /locus_tag="OO322_002235"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5907099.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12587..13165
                     /gene="lpcA"
                     /locus_tag="OO322_002236"
     CDS             12587..13165
                     /gene="lpcA"
                     /locus_tag="OO322_002236"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5907100.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13371..14138
                     /locus_tag="OO322_002237"
     CDS             13371..14138
                     /locus_tag="OO322_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5907101.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14109..14849)
                     /locus_tag="OO322_002238"
     CDS             complement(14109..14849)
                     /locus_tag="OO322_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5907102.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15233..15754
                     /locus_tag="OO322_002239"
                     /pseudo
     CDS             15233..15754
                     /locus_tag="OO322_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15930..16427
                     /gene="rayT"
                     /locus_tag="OO322_002240"
     CDS             15930..16427
                     /gene="rayT"
                     /locus_tag="OO322_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5907103.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16644..18356)
                     /locus_tag="OO322_002241"
                     /pseudo
     CDS             complement(16644..18356)
                     /locus_tag="OO322_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18328..19113
                     /gene="lafU"
                     /locus_tag="OO322_002242"
     CDS             18328..19113
                     /gene="lafU"
                     /locus_tag="OO322_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5907104.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19184..20239
                     /gene="dinB"
                     /locus_tag="OO322_002243"
     CDS             19184..20239
                     /gene="dinB"
                     /locus_tag="OO322_002243"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5907105.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20291..20584
                     /gene="yafN"
                     /locus_tag="OO322_002244"
     CDS             20291..20584
                     /gene="yafN"
                     /locus_tag="OO322_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5907106.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20587..20985
                     /gene="yafO"
                     /locus_tag="OO322_002245"
     CDS             20587..20985
                     /gene="yafO"
                     /locus_tag="OO322_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5907107.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20995..21447
                     /locus_tag="OO322_002246"
     CDS             20995..21447
                     /locus_tag="OO322_002246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5907108.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21693..22736
                     /locus_tag="OO322_002247"
     CDS             21693..22736
                     /locus_tag="OO322_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5907109.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22798..23412
                     /locus_tag="OO322_002248"
                     /pseudo
     CDS             22798..23412
                     /locus_tag="OO322_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23469..24926)
                     /gene="pepD"
                     /locus_tag="OO322_002249"
     CDS             complement(23469..24926)
                     /gene="pepD"
                     /locus_tag="OO322_002249"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5907110.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25188..25646
                     /gene="gpt"
                     /locus_tag="OO322_002250"
     CDS             25188..25646
                     /gene="gpt"
                     /locus_tag="OO322_002250"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5907111.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25738..26982
                     /gene="frsA"
                     /locus_tag="OO322_002251"
     CDS             25738..26982
                     /gene="frsA"
                     /locus_tag="OO322_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5907112.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27040..27441
                     /gene="crl"
                     /locus_tag="OO322_002252"
     CDS             27040..27441
                     /gene="crl"
                     /locus_tag="OO322_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5907113.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27480..28535)
                     /gene="phoE"
                     /locus_tag="OO322_002253"
     CDS             complement(27480..28535)
                     /gene="phoE"
                     /locus_tag="OO322_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5907114.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7057 a         7117 c         7597 g         6836 t
ORIGIN      
        1 tatacataag gatggtagga ggacgtattt attaaatcaa aatactggtt cgattacggc
       61 tgtgataaga tggaagtatg agtagtataa ggataatacc gttacagatg actggactat
      121 atgggaaaaa gctattttta tgcgagcgat atatttatct gttcagcagg catggaatgg
      181 gaaaataact tattctgttt ccggtgaatc tgagttcgct aaaaaatttc agggtaaagc
      241 tttgcctttt gatgtaagaa taatttctgc atctcagaat gaagactggt tggttattgc
      301 aacaaaggtt ttgcctggtg ctgatttaag aacatatgtt gattttaaaa acagtacggt
      361 tcatgttgat tcagcgggcc tggaaaaagt agcgaaatgt attaactgta ataatacgtt
      421 gcaagtaaat atacctcatg aagccggaca tgtacttggt tacttagatg atgattatga
      481 ttcttcgtct ccgtatgttg gagatattag tgggttgatg aatgtgggaa tggagctttg
      541 ggaacgatac cttaaaaacg caactataac gttgaatatc ataatgcctg aaactaaatt
      601 tactttattg aatgtaacca aatgaaattt ttctgtatta tagtgatttt cctttcaata
      661 aattacatag ctatagtgaa tgttttcaag tcttctgcag ttgctcaatc caccaaatat
      721 tatgaagaaa atgatataca tgatggaagt aatgacatct tttttatgaa gaaatatggg
      781 ccgcctgctc atgagttcgt gaggccacga tataaatttg ttggtgagta ttatatcggc
      841 ttgaatcaaa cttatcgcat aacagatcct gaatttcaag atgttcctat taaggaaatg
      901 ttctggaatt tgcaggggga tttgaatctg acatgttggt ttcattataa agacgggcaa
      961 tggagagtga ttacttatat tttttggcca ccaggggcta gattttaatc cggtattaaa
     1021 ggagcaaata tcatgagttt tgtatctacg ggtaataaac cagttggaaa cggtggaccc
     1081 gtcatcacca caccaccaat agctggcgaa agtggcggta tgagcacagg ttcagctgta
     1141 accgatgtat ctggtgccgc cgaggagatg gcagaacagg ctgcggcgga cctgtttggt
     1201 gcgctgccgg aaccgtcagg tctggttaaa gccgcagtag ctgctgcgca ggctgccgct
     1261 gcagcagcag ggatctcgga tatggctggt gcagttcagg atgcagcggc gtcgcttgca
     1321 gccggtgctc cgggggcgca taacgtcaca gtaagtggta gtgcggtgcc gccgcagatg
     1381 cttttgtttg caggcatgaa tggttcggaa gggcttggga atctgttttc ttacacagta
     1441 cagcttaaaa cgctggacgc cctgaacctg gggtacgttt cccccgctgc taacctgccg
     1501 cttcagctca tggtgggcaa agacctgtgc gtcagtatcg aactggacgg cggcggaaaa
     1561 cggtatatca gcgggctggt gacggcggcg agggtggtcg gccatgagag ccgttcggta
     1621 acgtatgaat tacgcattga gccatggctg aagctgctga cccataccag cgactacaaa
     1681 gcgttccaga acaaaaccgt ggtggatatt ctcgatgaag tactggatga atattccttt
     1741 ccggtggaaa agcggctggt ggaaaattat ccaccccgcg cctggcaggt gcagtatggt
     1801 gaaaccgact tcgatttcat ccagcgcctg atgcaggaat gggggattta ctggtggttt
     1861 gagcacagcg agaacagcca cacgctggtg ctggtggatg ccatcaatgg tcataaagcg
     1921 tgccctgact cgccgctggt ggagtggcac caggaagggc tgaaactgga taaggaattt
     1981 atccacacca ttacggccag tgagaggctg cgtactggaa agtgggtgat ggacgatttt
     2041 gattttatga agcctcgttc gctgcttaaa agtaccgtgg ccagtccgcg tgataccggt
     2101 catgctgaat acgagcatta cgaatggcct ggtgattact tcactacggg cgaaggcgag
     2161 atgctgacgc gcattcgtat ggaagcgcag cgcagcccag gcagccgggc acatggggcc
     2221 ggtcatatcc gtaccctcat gaccgggtac acctttacgt tgatgaatca cccgacggca
     2281 gaaattaatc aggaatatct gctggtacag accactctgt ttttacggga taatgcgcag
     2341 cacagcggtc aggatcagca ttttacctat gtcaccacgt ttgaactgca ccccaccagc
     2401 gaagtttacc gcccacaacg aaccctgagc aaaccccaca ccaaaggccc gcagagcgcc
     2461 atcgtcaccg gcccggcggg gcaggaaatc tggacggata aatatggccg ggtgaaggtg
     2521 cagtttggct gggaccgtta cggaaagaat gacgaaaaca gttcctgctg gatccgcgtc
     2581 agctatccgt gggcgggtaa aggattcggc atgatccaga tcccgcgtat tggccaggaa
     2641 gtactggtgg atttcaaaaa cggcgatccg gatttgccca tcatcgtggg gcgcacctat
     2701 aaccaggaca ccatgccgcc gtgggggctg ccgggcatgg cgtcgcagag tgggatattc
     2761 agccactcat tgcagggagg gccgactaac ggaaatatgc tgcgcttcga tgataaaacg
     2821 ggtgcggaag aggtgaagtt ccatgcggaa aaagacctca ataccacggt aaaaaacaat
     2881 gaaacccaca cggtcatggt cgatcgcata aaaaccattg tcaaaaatga aaccatcagc
     2941 gtgggagaag accgtaatgc caccatcaca aaaaatgatg gtctttccgt caaactggct
     3001 caaaccatca atgtcggaac gacatatcgt ctggatgttg gcgatcaatt tacccttcgt
     3061 tgcggcaatg cagcgcttgt attacacaag gatggctcga ttgagttttg cggaaaacaa
     3121 ctgttactgc acaccagcga tgttatgcaa ctcattggca gtggcattga tatgaatccg
     3181 gatggcggca cagccatgac tgcggatgat attgccccgt tacctacccc cgacgtttct
     3241 gaataaaaaa tataaaagga atttttctat gaccagcctt taccgtattc aggagggctg
     3301 ctttgccctg cctgaaactt ttcttgatcg caccgtcaat atcttcgtcc catcgggcaa
     3361 tgaaagggca actcccagtc tgaatatttt ccgcgatacg ctcaggccag atgaaaatct
     3421 gaccacctat attgaccgcc agatagcctt gatgaaaaaa aaaacctagg ccagcaccgg
     3481 gtactttcgc ggggaacggc actggcggga actggcgaag ctgccatgac aggtgaacag
     3541 attctcgcaa ctcatcgttc tggaaaaaca gaagtgtacc agcgtcaggc cgggtttatc
     3601 accgggccag caaaggtact aatgttgacg ctgaccactc agcgtccttt tgatgatcac
     3661 accgaccagc tatggactgc atggttgact agttttcagc ctgctaaaag ctaaaaaaaa
     3721 cagggaggtt ttgtcatgta tgaagccgca cgggtggatg accccatcta ccacaccagc
     3781 gcccttgccg ggtttttgat aggggccatc ataggtatcg ccattattgc cgtcgccagc
     3841 tttgcttttt ttacctgcgg ctttttggcc gggctgattt tgagttttct ggctgatcag
     3901 atagcttccg gagtcctgca actgggtgag gctattgggc gctcgataca ttccacagtg
     3961 ggaaaaattc tgaccggttc tgataatgtc agcaccaaca gtcgcccggc cgcccgggcc
     4021 gtactgagta cggtgatatg cgaagaccat tccccggaaa tccgcattgc ccaaggttcc
     4081 ggcaacatct acatcaacag ccagctgccc gtaaggatga ccacactgag tgtgatgcag
     4141 tgattgagga cggctccccc aatgtctttc tcggcggcgg tacgcagacg gtactggaaa
     4201 tcagcccgga aatccctgac tggctgcgtc aggtggtgga tgtgctgtat gtggtcgccg
     4261 gaatgcttgg cgggctggct ggcgcctggc gtcaggcggc aaagatgggg tcgaaatttg
     4321 gtacaaaatg tgcggcaaaa tttattggtg gcgaaatggt cgggatggcc atctctgaca
     4381 cagtcatggg attgttcagc aatccggtag atgtcaccac tgggcaaaaa attttgctgc
     4441 cggagacgga ttttaccctg ccaggacgcc tgcctgtcac ctgttcgcgc ttttacgcca
     4501 gccacctgga aactgaagga ttgctgggca gaggatggcg gctgaactgg gagataaacc
     4561 tgcgtgagga tgaaacatat atcaccttca tcggtgtaca gggtcgggaa ctgagttatc
     4621 cgaaagagat gctgataccg ggccaccaga tattcgaccc ggaagagcag ttttacctga
     4681 gccgtctgca tgacggatgc tatgtgctgc attacaccga ctgcagctat tacgtgtttg
     4741 atgaatttga tgaccacggc gtcgcgccgt tactttttat ggagacgccg taccggcagc
     4801 gtattgcttt cgggcgtgag aacggcaggc tggtgcgggt ggcctccagc agcggacacc
     4861 atctgttatt gcaccgcacg atgacgcaag caggggaacg cctgtcgcat attgaacttt
     4921 tgaagggcgg gcgtcccggt aacctggtgg aatatcgtta cgacgataac gggcaactga
     4981 ctggcgtggt gaaccgggct ggtgtgacgg tgcgccagtt tgcctatgaa aatggcctga
     5041 tgacagagca tcgcaacgcg acgggcttta cctgtaccta ccactgggaa gagattgaag
     5101 gtttcccgcg cgtggtggaa cacacgacca gcgacggcga ggattaccgg ttccactatg
     5161 attttgctgg cgggcagacg gtggtaaccg gcaggccgga gcagaagtgg cagtggtggt
     5221 ttgatgaaga gacgtatgtg actgcgcacc ggacgccggg cggcggaatg taccgcttca
     5281 cgtacaacga aaaccatttt ccggtagcgg tggaactccc cggcgaacgc agggtaacgc
     5341 tggaatacga caccctgagc cgggtggtga aagagacgga cccggcaggc cgggtgacac
     5401 agacgcagtg gaacggcagt tttgctgaaa tcacgcgccg ggcgctggat gacgaccacg
     5461 tatggaaggc ggattacaac gaacacggcc aggtgatacg ggagacggac ccggaagggc
     5521 gcgtcacgcg gtacgggtat gatgatcagg ggctgccgga gacggtctgc caccccggta
     5581 aacagcagga ccgttacacc tggaacgcgc tggggttact gagcagccac cgtcgcatca
     5641 cgggcagtgt gcagtcctgg cagtatacgc agcgcgggat gctggcgcgc catacggatg
     5701 aagagaaacg ggagacgcgc tggcagtaca ccccggaagg gctggttgcc agtctgagca
     5761 acggcaacgg ggctcagtac cggttcagct acgacggcga cggacggctg acgggagaac
     5821 agcgcccgga cggcctgata aggatgtttg cgctgaatgc ggacgggttt ccggtcatca
     5881 tcccgacgca gggcacagag ggcggggtgc gcaatgaaca gcaggagcgt gatgcgctgg
     5941 gccgcctgct gcgcagtgac acgcagcaca gtacacggac cttcagctac aaccgtcttg
     6001 accagataac ggaagtgacg ctcaccccca cggaagaagg ggaacggctg caccacatgc
     6061 aggcggacac ggtgcgtttt gcgtatgacc gcagcgggtg gctgacggca gagcacagcg
     6121 tgcacggcag tatcaaatac cggcgcgatg cgctgggcaa tcccaccgat atcacgttgc
     6181 cggacgggca gcatctcagt catctttact acggcagcgg gcatctgctc cagacggcac
     6241 tggacggtat cacggtcagt gaatatgagc gtgacagcct gcaccgtcag gtgatacgca
     6301 cgcaggggaa actggcgacg ttcagcggct acaacgccga caaccggtta agctggcagc
     6361 gcagcctgcc aggtggcagc cagaatccac agcaggcggt actcccccgg cgcagaacga
     6421 cagcgtgacg gcgcgggact atatctggaa cgcagacggt gaagtcggcg gcatcaatga
     6481 caaactgcgc ggttgcctgg tgttcagcta tgaccgcagc ggctggctga ccagccgcac
     6541 ggggcagatg tatgaccatg accattatta ttacgacaaa gcgggcaacc tgctgacgga
     6601 tgagtatcag ggagcggtga tggataaccg cctgccgggt tacgggcgtg accgctaccg
     6661 ctataacgaa tggggcgaac tgacagagcg cagggaccag cagctggagt ggaacgcgca
     6721 gggccagtta acgcgggtca tcagcagcaa cagcgagacg cggtatcagt acgatgcgct
     6781 gggcaggcgc atcagcaagg cgaccagcaa tctccatacc gaccggggag aacgaagccg
     6841 gacaacgttt gtgtgggaag ggttccggct gttacaggag acaacgtggc agggaaagcg
     6901 gacatacctg tacgatgcgg aacagccgta cacgccggtg gctgccatca cggggcgcgg
     6961 agagagccag aaaatctggt attaccacac ggacctgacg ggcacggtac acgaggtaac
     7021 cgcgccggac gggacgctgg tgtgggcggg ctaccaggcg ggatttggtg aaaaccgggg
     7081 cgacatcagc aacagcgggg cgtactttga gcagccgcag cggctgccgg ggcagtactt
     7141 tgacgaagaa acagggctgc attacaatct gttcaggtat tatgcgccgg agtgcgggag
     7201 gttcgtaagc caggacccga taggattaaa cggtgggttg aacttatatg cttatgcgcc
     7261 taatccgtta ggttggatcg atccgctagg actgaattct ttaggaatag attcaaataa
     7321 tatatttaga ggagatggta actataaagg tggcggaatt ggacagccaa agagtggcac
     7381 aatatcagca caggacatta ttgatcatct ctaagaaaaa ggtgatgggc gtttaacatc
     7441 ttttagtact aaaaaaacat ctagttccaa ctctcgtggt gcagattttt tctcaaaaaa
     7501 acatgtcttc aaagtggcag tttctgatct cgatgattta gttaaaaata atcagatcac
     7561 tgttttaaca caagaaaaag taaaagcaat atttggttta gaatcaagca agaaaattag
     7621 aaaagaactt ggtaatattc ttcagaatat ggctaggaat aatgaggtgt tagttcatgg
     7681 gcaaattctt ggaaataaag ttagccgatg taattagtta gtggagagga agttgtggac
     7741 gaaattagaa taagaattgc tggtaatacg gctgcgcctt gtttccatgt gctaaaaaat
     7801 aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt agattgtgta
     7861 tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc agaagagtta
     7921 cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa tttgaatgag
     7981 ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa cggcaatgaa
     8041 atagagtctg actaaatttt attgatatag caacgataat aaccggaaat ttcttccaga
     8101 tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc gtctcgcatc
     8161 cccttaactg gctgtttaac ctgataaagt tccctgcgca gttcctgtac ttcgttgtta
     8221 tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg ccgatctcaa
     8281 atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg ctgcggttgg
     8341 gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct ggcgtaatat
     8401 tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa cacaaccccc
     8461 actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc tctatattcc
     8521 cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc gtcggcggta
     8581 gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
     8641 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
     8701 gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac gtacagggcg
     8761 aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta gcacaccagc
     8821 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgc
     8881 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     8941 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     9001 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac gtcatcttgt
     9061 gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc cggtagaacg
     9121 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag gttggcagga
     9181 ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg cacggtgtac
     9241 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     9301 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     9361 tcgctaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     9421 aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg caggatgatt
     9481 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat cagatggtgc
     9541 aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     9601 taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     9661 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcgg
     9721 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     9781 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     9841 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     9901 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     9961 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc cgctttcacc
    10021 agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg cgccagttca
    10081 tccagacggg taaatggcag atttttaccc agctctttac agatccgctg atgaatcggg
    10141 tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt atgctcgctt
    10201 ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac ttgtaaaatc
    10261 ttcgccattt tatgatccag cttgtcagac ggtgccagat aatgacgtcc ggtcgggaag
    10321 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaattgtcc
    10381 attgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag cggcagatcg
    10441 gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag gtaaagctgg
    10501 cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct gccgcccagc
    10561 agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
    10621 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
    10681 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
    10741 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
    10801 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
    10861 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
    10921 ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg cccgcggtgg
    10981 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
    11041 taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat acgcgccagc
    11101 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagaa
    11161 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
    11221 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
    11281 ggcccgccga tgatgtaatc gatcggcacg aagacatctt taccgcgcgt cggtccgttc
    11341 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtt
    11401 gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt ttccgggtcg
    11461 gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat gtagcgtttg
    11521 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
    11581 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag tgcaaagcag
    11641 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
    11701 taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt aatcgccagg
    11761 atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc tgagaactcc
    11821 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
    11881 cacaactccg gtggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
    11941 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag gcgcggctgc
    12001 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
    12061 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
    12121 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
    12181 acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc aacgccgagg
    12241 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
    12301 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
    12361 ttacttgtag gaggtctgac cacttgtgat gatatggttg tagtggatgt aaaaacattt
    12421 agcaatgtgt ttacaatata attacaacaa agctcacatt gttgctgttt ttatccgcac
    12481 ttcaggtcaa aaagtcctgg tcatagcacc tgcccgtact tctcgctttt ggcggtatcc
    12541 ggtacactgc attttgtcta ttacatttat gctgaaggat atcctcatgt accaggatct
    12601 tattcgtaac gaactgaacg aagcggcgga aacgctggct aactttttaa aagatgacgc
    12661 caatattcac gccattcagc gcgcggcggt cctgttagca gacagcttta aagccggtgg
    12721 caaagtgctt tcctgcggca acggcggttc ccattgcgac gctatgcact ttgccgaaga
    12781 gttgaccggt cgctaccgtg aaaaccgtcc gggctacccg gcgattgcta tttctgacgt
    12841 tagtcatatt tcctgcgtcg gtaatgattt cggtttcaat gatattttct cccgctacgt
    12901 tgaagcggta ggtcgcgaag gcgatgtact gctggggatc tccacctccg gtaactctgc
    12961 aaacgtgatc aaagcgatcg cagcggcgcg tgagaagggg atgaaagtga tcaccctgac
    13021 cggtaaagac ggcggtaaaa tggctggcac ggcggatatc gaaattcgcg ttccgcactt
    13081 tggttatgcc gaccgcattc aggagattca cattaaagtg atccatatcc tgatccagtt
    13141 aattgaaaaa gagatggtta agtaagtctg gcgtaggccg gataaggcgt ttacgccgca
    13201 tccggcactt gtgcgctgat gcctgatgcg atgctgacgc gtcttatcat gcctacaaat
    13261 ctgtacccga accgtaggcc gaataattcg tttacgccgc atccgacctg aaaattctta
    13321 aatcaatctt cgccggggtc catgcgctcc cgctgttgtg gaggttaccc atgtgcgaat
    13381 tgctcgggat gagcgccaac gtccctaccg atatctgctt tagtttcacc ggacttgtac
    13441 agcgtggtgg tggaaccggg ccacataaag atggctgggg cattaccttt tacgaaggta
    13501 aaggctgtcg cacatttaaa gatccacaac ccagctttaa ttcccccatc gccaaacttg
    13561 tccaggacta cccgataaaa tcctgttcgg tggtggctca tattcgccag gctaatcggg
    13621 gcgaggtggc gctggaaaat actcacccat ttacccgcga gttatggggg cgtaactgga
    13681 cttatgccca taacggacaa ctgacgggct acaaatcact ggaaaccggc aacttccgcc
    13741 cggtcggtga aaccgacagc gaaaaagcct tttgctggct gctgcataaa ttaacgcagc
    13801 gttacccgcg cacgccgggc aacatggcgg cagtgtttaa atatatcgcc tcactggcgg
    13861 atgaactgcg ggcgaagggc gttttcaaca tgctgctttc ggacgggcgc tatgtaatgg
    13921 cgtattgctc gactaattta cactggatca cccgccgcgc gccgtttggc gtggcaacgt
    13981 tgctggatca ggatgtggaa atcgacttca gctcgcagac cacaccgaat gatgtggtca
    14041 cggtgattgc gacacagccg ctgacgggca atgaaacctg gcaaaaaatt atgccaggcg
    14101 aatggcgctt attttgcctc ggggagcgtg tagtttgatg ccagttgtgg ctgcaccact
    14161 tcgtggctta acggcttgct gaccacgtaa cggccattgg ccacagaaac ggtcggtggc
    14221 ttacgggttt gctcaaagta gtcgtagccc ggcttcagct gctcccagaa gtccttaaag
    14281 ttggaatatt tatggcgctt catattggcg tcggtcatgc ggaatgggta aatactcacc
    14341 tgcacgctcg gctgaccaaa caccagcgca ccagtaacga actggaagat ctcatcaata
    14401 ccctgattgg tcattgcgta gcagccgatg gatacacaat cgccgtggat catcaggtat
    14461 ttcccttcat aaccatgcgc acggtcataa gcattgggga atccaatatt aatcgctttg
    14521 tagtaacggc tgtctggttt taactgatta cgctggacgc tataaaaccc ttccgggctt
    14581 ttgaaatcgc cctgacgctg ttttggccct aagccgccgg aatatttaca gattttataa
    14641 ctgtcgagca gttgatattg ctcgcccatt ttgacgtaaa gatcgagcgt acgttcttcc
    14701 ttgaagatct ggatgtagac aggggatccc atcaactgct gcttatactc tttgctcact
    14761 ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg aaacgcacgg gatcaacaac
    14821 atcgcaagaa ttaatgcgat tttacgcata ctgcttattc cttgataaaa cggttacaca
    14881 cgccaggacg gcaaaatgga tcccaaatcg gaatagtctg gatttggaag gctcacatta
    14941 tcaccaaaag agttttacgc aagcctgtcg gcgctgggtt tacaaattta gcacaggggc
    15001 agttttcaat agtccgaaaa gcgcatgtat catttatttc ctaatattat gaaatctcaa
    15061 tagtaatatg caaataagca attaataatc ccgtttactc ctccggtctt ttaaaattct
    15121 caccttcatt cagccggttt atgtctttca tgtcttcgtt cttgctcggc agatttttgc
    15181 atccaggcgt gttttcgtta tgcgttttgc tccctttact cgccagcgcc accacaaccg
    15241 ggagcagata aaacagcatc cagaatggtt tcctgcgccg ctcaaggcga gtgacagacg
    15301 ctggcaggcg ctggcggaaa acaaccactt tttaagcagc gatcacctgc ataacatcac
    15361 cgaagtggcg attcaccgcc tggagcagca gcttggcaag ccttacgtct ggggcggcac
    15421 gcggcccgat caaggcttcg attgtagtgg gttagttttt tatgcctaca acaagatcct
    15481 tgaggcgaaa ctcccgcgca cggccaatga gatgtaccac taccgtcggg caacgattgt
    15541 ggcgaataac gacctgcgcc ggggagattt gctatttttc catatccaca gccgcgagat
    15601 tgccgaccat atgggggtgt atctgggcga cggtcagttt attgagtcac tgcgcaccgg
    15661 ggaaaccatt cgggtaagcc ggttagccga acctttctgg caggaccatt ttttaggcgc
    15721 gcgcaggatt ttgacggaag agatgatttt gtaggccgga taaggcgttt acgccgcatc
    15781 cggcggttgt gcacggatgc ctgatgcgac gctgacgcgt cttatcatgc ctacaaatct
    15841 gtgcgttttt gcgagccgct ttcccgatat aacacagcgt cattgtcggc ctttcctttt
    15901 cctcttaccg ttgagaaaaa ggagtcgcta tgtcggaata tcgtcgttat tacatcaagg
    15961 ggggaacatg gtttttcacg gtaaatttac gcaatcgtcg aagccatctt ctgaccaccc
    16021 agttccagac gctccgtaac gccattatta acgttaagcg agacagacct tttgaaatta
    16081 acgcctgggt cgttttgccg gagcatatgc attgcatatg gacattaccc gaaagcgatg
    16141 atgatttttc ctctcgctgg cgggagatta aaaagcagtt cacccatgct tgcggattga
    16201 aaaatatctg gcaaccgcgt ttttgggagc atgccattcg caatacaaaa gattatcgcc
    16261 atcatgttga ttatatttat ataaatccag agaaacatgg ctgggtaaag caggtgagtg
    16321 attggccatt ctcaacgttc catcgcgatg tcgcgcgagg gttatatccc atcgattggg
    16381 cgggagacat aacggattta agtgccgggg agcgtatcat tttataactg tgcgctggtg
    16441 ccggatgcgc ttacgcttat ccggcctaca aatcgtgctt taacggtagg ccggataagg
    16501 cgttcacgcc gcatccggca gttatgcgca gatgcctgat gcgacgctgg cgcgtcttat
    16561 catgcctaaa aacttgtgcc tgaacggtag gccggataag gcgtttacgc cgcatccggc
    16621 aattgtgcac cgatgccgga ttcttacatc aacgccccca taatcttcaa ctccagctca
    16681 tccggcactt cgttatacga cagcacatgc agccccggcg caaacaacct tgcataacgc
    16741 ggcagcaaag ggcgtagctg cggcggcacc agcagcaccg gatctttccc cgccgctttc
    16801 atctgctctt tcacctgtgg catcgtgctc tggaactggt tgagcatatt cgggtcaacc
    16861 ggcacgctgt cgagcatcac tttgccggcc tgttgcgcct gattcactac gttggtcagc
    16921 agattttcca gctcattatt cagcgtatac accgtcagct cctgcttgcg aacgaacgga
    16981 tgagtaatgc tgcgccgcag cgccaggcgc acatcggccg ccagcagaat atgatcttta
    17041 gtcaccgtgc tactggcgac cagcacggtg gcgatggtga cgatatcgcg cagggaaacg
    17101 ccttcggtca gcagcgcccg gtacactttc agcaattggc tgtaattgag cgccgcgctt
    17161 aaatcctccg ccaggcgcgg tgccatcgac gacaaacggt tatgcaactg cgtgatgtca
    17221 tcatagttaa acaaatcagg aatatagctg cgcacaatct tgttcacatg ggtggcaatc
    17281 acactggcgc tgtcgatcac ctgatacccc atattcagag ccttcgcttt ctgcgcaggc
    17341 tggatccagg tgaccggtat cccgtacgcc gggtcgttcc ccagcacgcc gtcaatctcg
    17401 ccgtaggttt cgctggaggg cagcgccatc agtttatccg ccggaatatc cgcttcatca
    17461 gcctcaatgc cgttgatgaa aatagcgtac tggctgggct tgaggcggaa gttttcccga
    17521 atgcggatct ccggcagcag cacgccgtta ccgtcggaaa tcacctgccg aacgccgcga
    17581 atccgctggg tgagcgggtt gccctgcgct ttgtccacca gcgccaccag cttgtagccg
    17641 aggcttaagc tgatgggctc gatcagcgga atagtttccc aactgacctg ttgttcgctg
    17701 gtttcagtga tggtgcgggt cagcgtttcg aggcttttct cttccgcctc cgccgcctgc
    17761 gggcgtttgc tcatccgcca gccggtaaag ccaagcaggg cgctgaacag caaaaacggc
    17821 agatgcggca tccccggcac caccgccagc acgaacatga tcccggtggc ggtgtagagc
    17881 accgacgggc ttgccagcaa ctggtggcgc acgtcatggg cgatatcgcc gttgtcgctg
    17941 acgcgggtga cgataatcgc cgccgcggtg gagagcagca gggaagggat ctgcgccacc
    18001 aggccgtcgc cgatggtcat cagcacgtat tgctggaagg cggcgtcggc gctcaggttg
    18061 tatttgaaga tgccgataca gacgccgccg ctcaggttga tcgccagaat catcatcccg
    18121 gcgatggcgt ctccgcgtac aaacttcgat gccccgtcca tcgcgccgta gaaatcggcc
    18181 tcgctggcaa catctttacg ccgggtttgc gcctgcgcct ggttgatcaa tccggcgtta
    18241 agatcggcgt caatcgccat ctgtttgccg ggcatcgcgt ccagggtaaa gcgggcagaa
    18301 acctcggaaa tacgctcggc ctctttggtg acgacgataa agttgattgt caacagcgtc
    18361 agtaaatccg agcgtgaaag cattatcgcc gcgctgcacg ggcagtcgat ttttaacggt
    18421 ggtgggctgt cgccgttaaa taaaatcagc ccgtctcatc cgccaaaacc tgccaccgtg
    18481 gcggtgccgg aagagacgga gaaaaaagcg cgtgatgtga atgagaaaac ggcgctgctg
    18541 aagaagaaat ccgccaccga gcttggcgag ttggcaacca gtatcaacac cattgcccgt
    18601 gatgcgcata tggaagcgaa tctggagatg gagattgttc cccagggatt acgcgtgctg
    18661 attaaagacg accagaatcg caatatgttc gaacgcggca gcgcgcagat tatgccgttc
    18721 tttaaaactt tgctggtgga gctcgcgcca gtgttcgact cgctctataa caaaattatt
    18781 attaccgggc ataccgatgc gatggcctac aaaaacaata tctacaacaa ctggaacctt
    18841 tcgggcgatc gcgcgctttc agctcgtcgg gtgctggaag aggccggaat gccggaagat
    18901 aaagtgatgc aggtaagcgc aatggcggac cagatgctgc tggacgcgaa gaatccgcaa
    18961 agcgcgggca atcggcgtat tgagattatg gtgctgacca aaagtgcgtc cgatacgctg
    19021 tatcaatact ttggtcagca tggggataaa gtggtgcagc cgctggtgca aaagctggat
    19081 aagcagcagg tgctttcgca gcgaatgcgc taaatgctga atctttacgc atttctcaaa
    19141 acctgaaatc actgtatact ttaccagtgt tgagaggtga gcaatgcgta aaatcattca
    19201 tgtggatatg gactgctttt tcgcagcggt ggagatgcgc gacaatcccg ctctgcgcga
    19261 tatccctatt gctattggcg gcagccgcga acgtcggggg gtgatcagca ccgccaatta
    19321 tcccgcgcgt aaatttggcg tacgtagcgc tatgcctact gggatggcgc tcaaattatg
    19381 cccacatctc accttgcttc cggggcgctt tgacgcctac aaagaagcct caaatcatat
    19441 ccgtgaaatc ttctcgcgct acacctcgcg cattgaaccg ttgtcactgg atgaggctta
    19501 tctcgatgtc accgatagcg tccattgcca cggttctgcg accctcatcg cccaggaaat
    19561 ccgccagacg atcttcaacg agctgcaact gacggcatct gcgggcgtgg caccagtaaa
    19621 gtttctcgcc aaaatcgcct ccgacatgaa taaacccaac ggtcagtttg tgattacgcc
    19681 ggcagaagtt ccggcatttt tacaaacctt accactggaa aaaatccccg gcgtcggcaa
    19741 agtctcggcg gcaaaactgg aagcgatggg gctgcggacc tgcggtgatg tacaaaagtg
    19801 tgatctggtg attctgctta aacgctttgg caaatttggc cgcattttgt gggagcgtag
    19861 tcaggggatt gacgagcgcg acgttaacag cgaacggtta cgaaaatccg tcggcgtgga
    19921 acgcacgatg gcggaagata ttcatcactg gtctgaatgt gaagcgatta tcgagcggct
    19981 gtatccggaa cttgaacgcc gtctggcaaa ggtaaaacct gatttactga ttgctcgcca
    20041 gggggtgaaa ttaaagttcg acgattttca gcagaccacc caggagcacg tctggccgcg
    20101 gctgaataaa gctgacttaa tcgccaccgc gcgtaaaacc tgggatgaac gccgcggcgg
    20161 gcgcggtgtg cgtctggtgg ggctgcatgt gacgttgctt gacccgcaaa tggaaagaca
    20221 actggtgctg ggattatgat gtatactatt atgtatattc tggtgtgcat tattatgagg
    20281 gtatcactgt atgcatcgaa ttctcgctga aaaatcggtc aatatcactg agttacgtaa
    20341 aaacccagct aaatacttta ttgatcaacc agttgcggtt ctttctaata atcgccccgc
    20401 aggatatctc ttaagtgcca gcgcattcga agcgttaatg gacatgcttg ctgaacaaga
    20461 ggagaaaaag cccataaagg cgcgctttcg tccaagtgct gcaagattag aggaaattac
    20521 acgccgcgct aaacaatatc ttaatgatat gacggatgat gatttcaatg actttaagga
    20581 ataaggatgc gggtattcaa aacaaaactt attcgcctgc aacttacagc agaggaactt
    20641 gatgcgttaa cggcggattt tatttcctat aagcgtgacg gtgttttgcc agatatattt
    20701 ggtcgcgatg cactctacga cgactcgttt acctggccat taatcaaatt tgagcgagtt
    20761 gctcatattc atctggcaaa tgtgaataat ccatttccgc cacagttgcg ccaattcagc
    20821 agaacgaatg acgaagcgca tttggtatat tgtcagggtg cgtttgatga gcaagcatgg
    20881 ttgctcattg ccattctgaa gcctgaacct cataaactgg ctcgagataa caaccaaatg
    20941 cataaaattg gaaaaatggc agaagcgttt cgcatgcgtt tttgaattta tattatgaat
    21001 aacatacaaa taagagatta tcagcctggc gattttcagc aactatgcgc tattttcatt
    21061 agagcggtta cgatgaccgt cagtcagcat tattcactac aacaaattgc cgcctgggcg
    21121 cagattgacg aatctcgctg gaaggagaaa ctcgcgaaat cacaagtgcg ggttgcagtt
    21181 attaatgcaa aactggtcgg ctttattacc tgcgttgaac actatatcga tatgttattt
    21241 gttgaccctg aatacacccg ccgtggggtt gccagcgctt tgttaaaacc ttttattaag
    21301 tctgaatccg aacttacggt ggacgcaagc ataaccgcaa aacccttttt tgaacgttat
    21361 ggttttcaga cagttaagca gcagcgcgtt gaatgccggg gagcgtggtt tattaatttt
    21421 tatatgcgtt ataaaccgca acattaaatc cagcttgcaa tgaaaataac gcccgcctag
    21481 tatgtgcgcg ttttcttatt ccacaaactg caaggaggta aatcatgaca aatcctttat
    21541 caatgactct ttgcagacct ttccaggatt aattcttttt ttcttgccct ggattcgtct
    21601 gccatttcct gattttttaa ttgttactct caataattca agtcgcagca catgcaactt
    21661 gaagtatgac gagtctattt acggaatggt atatgggcaa ttatattcgt cccttatccg
    21721 atgcggtatt taccatcgca tctgatgacc tgtggatcga gagtttagcg atccaacaat
    21781 tacacaccac ggcaaattta cccaacatgc agcgcgtagt ggggatgcca gatttacacc
    21841 ccggacgcgg ctacccgatt ggcgcagcgt tcttctccgc aggccgtttt tacccagcac
    21901 tggtcggcaa tgatatcggc tgtggcatgg cgctatggca aacagatatt ctcgctcgca
    21961 aatacaacgc cgataagttt gaaaagcgat tatcagcttt ggatgacgtt gctgaagaaa
    22021 gctggctgga ggaaaacctg ccgtcagcat ttgcacagca tccgtggcgc agctcgcttg
    22081 gctctatcgg tggtggcaat catttcgctg aactgcaaca agttgatcaa atcatcaacg
    22141 ctgaactgtt tgcactggca ggtctggatg cgcagcatct gcaactgctg gttcatagcg
    22201 gctcgagggg gctagggcag tctattttac agcggcatat tgcctcgttt tcgcatcatg
    22261 gtttgcctga aggcagtgac gacgcgctgg cgtttgcgcg ttttaatcgc cacctgatcg
    22321 ctttgcgcat aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata
    22381 actttgttag cgcgtgtcga atcggtgatc aacaaggggt cttacatcgt aaaggtgcca
    22441 cgcctgatga ctgtggtctg gtggttattc ccggttcacg cggtgattac tccaggctgg
    22501 ttcagcccgt caggagtgag gaaacattgc attcgctggc gcatggggct gggcgtaaat
    22561 gggggcgcac cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg caactctcgc
    22621 gtactgaact tggcagtcgg gtaatttgtc gcgataaaca actcatcttc gaagaagcgc
    22681 cacaagccta taaatcggct gaaagtgtgg tgcaatgtca gggcaggccg gattaattat
    22741 tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga aaaaaggatg
    22801 atcttgctat aactctcctc agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag
    22861 gcgctggaca ggctaattaa agaagctgcc cgacaagacg tcgcggtaac ggtgctggaa
    22921 acagaaacag gccgctactc tgacacgctg cgttcggcgc tgatttctct ggatggcgac
    22981 aacgcatggg cgttaagcga aagctggtgt ggcactattc agtggatttg cccgagcccg
    23041 tatcgcccac atcacgggcg taaaaactgg tttctgggca ttgggcgttt taccgctgat
    23101 gagcaggagc aatccgatgc gatccggtat gagacgctgc gttcgtcggg gccgggcggt
    23161 caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc cggtattagc
    23221 gtgaaggttc agtcagagcg cagccagcat gctaacaaac ggctggcgcg attgctgatt
    23281 gcctggaagc tggatcagca gcaataggaa aatagcgcgg tgctgaaatc acagcggcga
    23341 atgttccatc accagattga acgtggcaac ccgcgacgaa cgtttacagg gatggctttt
    23401 atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa
    23461 tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt ccagtaatga
    23521 cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat agttggcccg
    23581 atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc caggcccgcg
    23641 tggataatct ggatgttcgg cgtcttgttg aacaggcgct gataggtttc acgtaccaga
    23701 tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt
    23761 ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc tttaccgctg
    23821 tcgattagtg aacggatcag gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca
    23881 ccgacgttca gggaggtttc gaccacacct tttgccacgt cggaattgcg gatcacaccg
    23941 ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct
    24001 ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg
    24061 tttttcagga tctcctgata ggtattcacc agagatttca gtgcgtcgac tttatcagct
    24121 gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg
    24181 aaatcgataa ggcgcaggtc cagttcttct gcatgacccg ccaggaagcg caccagcagt
    24241 ttgttggcat tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc tttcagacct
    24301 tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg
    24361 ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag
    24421 tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc
    24481 ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac cacgttttcg
    24541 tcagccagaa ccgccagtgc agaggccata ccaatgccgt tatccgcacc cagcgtggtg
    24601 ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca
    24661 tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt
    24721 ttacgatttt ccatacctgc ggtagcaggt tgacgaatca ggatattacc tacctgatcg
    24781 cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct
    24841 tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt
    24901 ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt
    24961 attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg
    25021 tattgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
    25081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
    25141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
    25201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
    25261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta ccgggggcgt
    25321 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
    25381 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
    25441 tcgttattga tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc
    25501 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
    25561 atgttgttga tatcccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat
    25621 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcattgccg ggcgttgttc
    25681 tttttaactt caggcaggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
    25741 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
    25801 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc tcttgatggt
    25861 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt
    25921 gacccacgtg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc
    25981 gacgatgatt tatacgatac cgtgattggc taccgtggtg gcaactggat ttatgagtgg
    26041 gccacccagg cgatggtgtg gcaacaaaaa gcctgtacgg aagaagatcc gcaactcagt
    26101 ggtcgacact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa
    26161 ggagatgacc tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc
    26221 cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc
    26281 accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt
    26341 ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc
    26401 ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc
    26461 acccaggact ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg
    26521 gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt
    26581 ctggcatacc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat
    26641 accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg
    26701 gcgagtcgtt tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc
    26761 tattcattaa aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc
    26821 tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct
    26881 gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt
    26941 cttcaggaaa tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc
    27001 caatttggta aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac
    27061 ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag
    27121 tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca
    27181 ccggaagtgc gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt
    27241 acctacagct accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta
    27301 aaagatactg aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg
    27361 cgtgaactgc tgacgacgct gaatctgaag ctggaaccgg cgaatgattt tcgtgatgaa
    27421 ccggtgaagt taacggcgtg agtgaaatgt gccggatgca tcacatccgg caatattcat
    27481 taaaactgat acgtcatccc aaccgcgaca gtatcatcat tattaatatt cagcttatta
    27541 tcgctatcca gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa
    27601 tacgtagcac cgacgtcgat ataattgacc agatcttcat caccgatgcc ctcaatatct
    27661 ttccctttcg ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga
    27721 gcgaccgctt caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg
    27781 cgtgtttcag aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt
    27841 gcccatgctt ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga
    27901 tctgagttgg tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat
    27961 gacgtgccga agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga
    28021 tattgcaggg ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc
    28081 gccagaccgc tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca
    28141 aattccggga acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca
    28201 taatcgaaag aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc
    28261 tgtgcagtat cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca
    28321 gtcagttgat cgttaatttg agtttcacct ttaaaaccaa aacggatata actctggtcg
    28381 ccatctttac tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc
    28441 agtttattac cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg
    28501 cccatcacca ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc
    28561 gcgaatattc agcgggagag ccccgttgaa aacaggaaag tttttaa
//
LOCUS       DAJTRM010000050        28177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRM010000050 DAJTRM010000000
VERSION     DAJTRM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.050"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO322_002254"
                     /pseudo
     CDS             complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO322_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1013..2035)
                     /locus_tag="OO322_002255"
     CDS             complement(1013..2035)
                     /locus_tag="OO322_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5907115.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2049..3551)
                     /locus_tag="OO322_002256"
     CDS             complement(2049..3551)
                     /locus_tag="OO322_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5907116.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO322_002257"
                     /pseudo
     CDS             complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO322_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4957..5487
                     /gene="ppa"
                     /locus_tag="OO322_002258"
     CDS             4957..5487
                     /gene="ppa"
                     /locus_tag="OO322_002258"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5907117.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5867..6208)
                     /locus_tag="OO322_002259"
     CDS             complement(5867..6208)
                     /locus_tag="OO322_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5907118.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO322_002260"
     CDS             complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO322_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5907119.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO322_002261"
     CDS             complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO322_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5907120.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11926..12564
                     /gene="msrA"
                     /locus_tag="OO322_002262"
     CDS             11926..12564
                     /gene="msrA"
                     /locus_tag="OO322_002262"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5907121.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12887..14230
                     /locus_tag="OO322_002263"
     CDS             12887..14230
                     /locus_tag="OO322_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5907122.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14292..14498)
                     /locus_tag="OO322_002264"
     CDS             complement(14292..14498)
                     /locus_tag="OO322_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5907123.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14823..15377
                     /locus_tag="OO322_002265"
     CDS             14823..15377
                     /locus_tag="OO322_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5907124.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15440..16378)
                     /locus_tag="OO322_002266"
     CDS             complement(15440..16378)
                     /locus_tag="OO322_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907125.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO322_002267"
     CDS             complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO322_002267"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5907126.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17520..19463
                     /gene="cpdB"
                     /locus_tag="OO322_002268"
     CDS             17520..19463
                     /gene="cpdB"
                     /locus_tag="OO322_002268"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5907127.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19589..19969)
                     /locus_tag="OO322_002269"
     CDS             complement(19589..19969)
                     /locus_tag="OO322_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5907128.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20058..20918
                     /gene="qorB"
                     /locus_tag="OO322_002270"
     CDS             20058..20918
                     /gene="qorB"
                     /locus_tag="OO322_002270"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5907129.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21027..21992
                     /locus_tag="OO322_002271"
     CDS             21027..21992
                     /locus_tag="OO322_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5907130.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22100..22762
                     /gene="ytfE"
                     /locus_tag="OO322_002272"
     CDS             22100..22762
                     /gene="ytfE"
                     /locus_tag="OO322_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5907131.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO322_002273"
                     /gene_synonym="dagA"
     CDS             complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO322_002273"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5907132.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO322_002274"
     CDS             complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO322_002274"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5907133.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25366..25911
                     /locus_tag="OO322_002275"
     CDS             25366..25911
                     /locus_tag="OO322_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5907134.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25895..26542)
                     /locus_tag="OO322_002276"
     CDS             complement(25895..26542)
                     /locus_tag="OO322_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5907135.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26754..27545
                     /locus_tag="OO322_002277"
     CDS             26754..27545
                     /locus_tag="OO322_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5907136.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27555..>28177
                     /locus_tag="OO322_002278"
     CDS             27555..>28177
                     /locus_tag="OO322_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5907137.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILA"
BASE COUNT         6916 a         7512 c         6944 g         6805 t
ORIGIN      
        1 atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga ctcttgtcac
       61 tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg ctataaaaat
      121 aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc cgtcaaagtt
      181 gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa gcaccgtccc
      241 gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt ccagttccac
      301 acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa tccccgccag
      361 tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tagaaatccc
      421 catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc caaaacgggt
      481 acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc ccatcgcact
      541 aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg
      601 gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc
      661 cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc ccataaatgc
      721 gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg agaggccgaa
      781 atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac cgacggagag
      841 atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc caagaaaggc
      901 gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa actgggtcag
      961 gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca agttacgttt
     1021 tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga acaatcagca
     1081 cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag cccgaaagca
     1141 aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc agcaggttaa
     1201 agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg tccagctcca
     1261 gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg ataatgcccg
     1321 caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt cgcgtgttta
     1381 ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac atccccagcg
     1441 ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata atgaccggcg
     1501 ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag ttaaacgtga
     1561 cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg
     1621 tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac aatcccgcca
     1681 ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc gcgaccgtca
     1741 tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga tcgataccac
     1801 cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct gcacggttaa
     1861 gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc cagaaatgcg
     1921 gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc ggcatccccg
     1981 tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga ggcatcacac
     2041 tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg tgccagcggg
     2101 atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata gcccaccagc
     2161 tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag cgtttcaatc
     2221 aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc atcaaggatc
     2281 agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg attgccaccg
     2341 gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag
     2401 cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc gcgctgggcc
     2461 tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc atcggttttc
     2521 ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga tcgcagggtt
     2581 tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc gaagatcact
     2641 tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat ctcgccgggg
     2701 cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt gaacgcggca
     2761 acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg
     2821 cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc ccgacagcct
     2881 acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac ctgatcgaga
     2941 aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat caggccaaat
     3001 aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat cagcactttg
     3061 gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac cgaaaaacgg
     3121 ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc ggtggcgcgc
     3181 ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc tatgaataga
     3241 ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt gccgataccg
     3301 agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag ccagatggtg
     3361 ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt tcccgccccg
     3421 ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac gttgtctaac
     3481 gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc ctggtgttgg
     3541 tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga taagacgcgg
     3601 taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg
     3661 cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa
     3721 ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg
     3781 catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg ccatgttcgg
     3841 cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga tgtccggtac
     3901 accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt ctttaattgc
     3961 ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc
     4021 gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc gggtgaagtc
     4081 acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc
     4141 tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca
     4201 tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt
     4261 gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag
     4321 caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc aacccgtcgc
     4381 aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac gtaccgcttt
     4441 aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc cgcgtttttc
     4501 ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg atccgacctg
     4561 cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg ccgagactgc
     4621 agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt
     4681 gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg cgttacatca
     4741 cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat
     4801 gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa
     4861 aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatacttg
     4921 gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg
     4981 ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg
     5041 atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc
     5101 gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac
     5161 ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc
     5221 cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa actggttgcg
     5281 gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcct
     5341 gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc
     5401 aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct
     5461 tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc
     5521 ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc caccatcatt
     5581 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
     5641 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
     5701 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
     5761 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
     5821 tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac ttatccctgt
     5881 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
     5941 tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
     6001 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
     6061 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
     6121 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
     6181 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
     6241 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
     6301 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
     6361 tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt cgagcgctaa
     6421 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
     6481 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
     6541 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
     6601 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat cgtcttctgt
     6661 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
     6721 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
     6781 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
     6841 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
     6901 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
     6961 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
     7021 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
     7081 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
     7141 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
     7201 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
     7261 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
     7321 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
     7381 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
     7441 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
     7501 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
     7561 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
     7621 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
     7681 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
     7741 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
     7801 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
     7861 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
     7921 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc gggtattgct
     7981 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
     8041 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
     8101 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
     8161 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
     8221 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
     8281 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
     8341 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
     8401 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
     8461 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
     8521 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
     8581 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
     8641 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
     8701 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
     8761 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
     8821 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
     8881 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
     8941 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
     9001 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
     9061 acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg gccagttgtc
     9121 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
     9181 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
     9241 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
     9301 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
     9361 ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga ccacttcttc
     9421 ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
     9481 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
     9541 ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg tgatgggata
     9601 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
     9661 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
     9721 gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg cttttaccgc
     9781 cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
     9841 cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
     9901 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
     9961 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    10021 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    10081 ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg gcgaatatcg
    10141 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    10201 tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc gttggcgtat
    10261 ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc gacgaagaaa
    10321 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    10381 gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc ctggaaaacg
    10441 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    10501 tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    10561 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    10621 aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc caccagcgta
    10681 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    10741 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag ggtctgttcc
    10801 ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat ccacggcttt
    10861 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    10921 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    10981 ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga aagtcggcgg
    11041 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    11101 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    11161 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    11221 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    11281 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    11341 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    11401 ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    11461 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    11521 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    11581 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    11641 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    11701 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc cctggaacgc
    11761 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    11821 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    11881 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    11941 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    12001 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    12061 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    12121 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    12181 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    12241 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    12301 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    12361 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    12421 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    12481 gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg cggaattggc
    12541 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    12601 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgcccaaatt
    12661 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    12721 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    12781 actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    12841 atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt taaacagtat
    12901 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    12961 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    13021 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    13081 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    13141 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    13201 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    13261 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    13321 cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    13381 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    13441 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    13501 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    13561 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    13621 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    13681 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    13741 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    13801 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    13861 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    13921 tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg acgacgtcat
    13981 gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca tcggcggctt
    14041 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    14101 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    14161 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    14221 cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca
    14281 tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt aacttcggac
    14341 atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc cttatcaaat
    14401 tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt tacgtacttc
    14461 gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg ctgagtatta
    14521 cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta atgcttagtg
    14581 actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct aaaggttaca
    14641 attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt catttttcat
    14701 atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg ataaatagca
    14761 catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa gaaggaaaca
    14821 ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg atggcttccg
    14881 cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat cggggcgagt
    14941 tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag ttagtgggaa
    15001 aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa aacgcgacgc
    15061 tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc accaccattg
    15121 ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt ctggagagta
    15181 ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc gcgcgcggtg
    15241 cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac ggtcgcgtgc
    15301 aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg gacctgctgc
    15361 aaaagttgct caaataatgg caataacata aattctggaa aaataatttt cccctatcgt
    15421 tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat actggtaacg
    15481 ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa atcttttctt
    15541 tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact tgttcggcta
    15601 attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag taatcttcat
    15661 cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag attttaagcg
    15721 caaagcccaa agattctatg actatggcaa aattatcatc aattattttt tcgtaaatat
    15781 accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat ttaccccgtt
    15841 ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca ccaaacgcct
    15901 ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc aaaatatcac
    15961 aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa tccatgaatg
    16021 agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg cctcgcaatg
    16081 ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa ttatccatgg
    16141 cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca atctgtgttg
    16201 cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc caatcttttt
    16261 tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt gtatgcgatg
    16321 actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg ggtaacatat
    16381 ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt atagaagtaa
    16441 actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt tttgcacttt
    16501 aaatacaatc tctttttatt tataactttt taggttggga taaatcagga tggcagaatc
    16561 aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa ccccggattc
    16621 aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct
    16681 ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt tggtccgaag
    16741 cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg
    16801 gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg
    16861 gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca
    16921 ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc
    16981 gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat
    17041 tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc
    17101 tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac cggaatatcc
    17161 ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc cgctgccgtt
    17221 accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc
    17281 ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct
    17341 cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc
    17401 caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat
    17461 aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga
    17521 tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg aatgcagcga
    17581 cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt
    17641 attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg
    17701 atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga
    17761 gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt
    17821 ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact
    17881 acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca
    17941 acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg
    18001 aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc
    18061 cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata
    18121 ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg
    18181 tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag
    18241 tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg
    18301 ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa ggcacgctga
    18361 atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac
    18421 aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga
    18481 tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac
    18541 tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg
    18601 acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca
    18661 acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc
    18721 cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct
    18781 atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc
    18841 ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct
    18901 ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca ctcatcaact
    18961 gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg
    19021 ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga
    19081 ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctggttgcca
    19141 ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt
    19201 ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac
    19261 gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg atagctgccg
    19321 ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcgtttatta
    19381 aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt
    19441 atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca accgcatccg
    19501 gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc atgaaccgta
    19561 gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca
    19621 cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct
    19681 cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac gcgatacggt
    19741 taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt tcactcaccc
    19801 caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa
    19861 tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg
    19921 caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt tcatacttac
    19981 ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa
    20041 aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata
    20101 ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc
    20161 cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct gactacagcg
    20221 atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca
    20281 gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg
    20341 gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg
    20401 ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc
    20461 tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg
    20521 gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg gcagattatg
    20581 cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg
    20641 cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca
    20701 aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg
    20761 gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg
    20821 gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg acaacgttag
    20881 ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc
    20941 gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc
    21001 ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctatacgcc ctgttagcag
    21061 ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt
    21121 tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt gcctggctgg
    21181 gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg atgctcacta
    21241 tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt actggcgcgc
    21301 ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc
    21361 tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca ctgatttgta
    21421 ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc cccgattttg
    21481 actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat
    21541 ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt
    21601 gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat ctcgtcgcct
    21661 gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc cgtccgctgg
    21721 tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc gcactctgct
    21781 ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt gttttcgaaa
    21841 cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg ctaatgacgc
    21901 tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa
    21961 aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta aataagcctc
    22021 tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat cttatcttat
    22081 taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac tggcgctctc
    22141 tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct gtggcggtaa
    22201 gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca ttgaagctga
    22261 actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc cgctggcaga
    22321 aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc tgccggagct
    22381 gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg
    22441 gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga tgaaagaaga
    22501 gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg ggccaatcag
    22561 cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca aacacactac
    22621 caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt ataacggcat
    22681 taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg tactgttccc
    22741 acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc cagcatgata
    22801 atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc agcccaaccc
    22861 cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt
    22921 cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca gcttgccgag
    22981 cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt acaccaggta
    23041 agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg ttgtaatcat
    23101 cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc cgagcagaca
    23161 gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc
    23221 tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag agaagacacc
    23281 gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc tggcagccgc
    23341 aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga ctaccgaact
    23401 ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa tgatacggat
    23461 cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt ccgcagctgt
    23521 tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa
    23581 gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga atgacgcttc
    23641 cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga caatcagcga
    23701 cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga acattttcac
    23761 ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga cccagtcgga
    23821 gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg ccataccggt
    23881 tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg gccccagtaa
    23941 atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca attcccccat
    24001 tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa tgatcgacgg
    24061 cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa tggcacctcc
    24121 aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact gttcagccgg
    24181 agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc ctgtacctgt
    24241 ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg
    24301 actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa
    24361 ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac
    24421 acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg
    24481 ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca
    24541 gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct gcgccgcgct
    24601 cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag
    24661 tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac
    24721 gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa
    24781 cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc
    24841 cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacgcctt
    24901 cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac
    24961 gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg
    25021 caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt
    25081 gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg
    25141 tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt
    25201 ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc
    25261 aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc gttagcttat
    25321 tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc cgggcgcttt
    25381 gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg cttcatggac
    25441 ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt ggtggtcgga
    25501 tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga agcgcagtta
    25561 gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac ccaaacgcaa
    25621 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    25681 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    25741 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    25801 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    25861 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    25921 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    25981 ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg atcaaataat
    26041 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct ggcttaaaaa
    26101 tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga gcatggttaa
    26161 tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt tcactatgca
    26221 caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca ataattttct
    26281 caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt tgtcgaatat
    26341 cgttccaggt atattccagc acttcgataa ataaattttc tttattacca aagtggtgat
    26401 aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg gccccttcat
    26461 aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc aggctctgta
    26521 cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg actatcataa
    26581 cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat caggttatca
    26641 aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt ttatcctcca
    26701 gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat tgtatgaaat
    26761 ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag ggctgtgcgc
    26821 aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat gccgacagcg
    26881 tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa gtgatttgct
    26941 tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg gcgcaaaacc
    27001 agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa aaacgcggtg
    27061 acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac acccgcgcgc
    27121 cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa ccggaagcgg
    27181 agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg ctggcgatga
    27241 atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt ctgtttgctg
    27301 ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc atcaaaactg
    27361 atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt ctggtgccgt
    27421 ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg gcggaaggta
    27481 aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt atgccgcgct
    27541 tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt atcaataccg
    27601 cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc gcacgcggta
    27661 ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag caaatcaccc
    27721 gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact tactacaccg
    27781 cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg ctggacgacg
    27841 cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca acgggcacta
    27901 aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg ctgccacatt
    27961 cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca gctgccgatc
    28021 gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc gatcccgatg
    28081 gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat ccggatctcg
    28141 ccagtcagat cctgcgtgcc ggaaaacgca tcctcgc
//
LOCUS       DAJTRM010000051        28119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRM010000051 DAJTRM010000000
VERSION     DAJTRM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.051"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..161)
                     /locus_tag="OO322_002279"
     CDS             complement(<1..161)
                     /locus_tag="OO322_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5907138.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMT"
     gene            complement(267..1283)
                     /gene="phnD"
                     /locus_tag="OO322_002280"
     CDS             complement(267..1283)
                     /gene="phnD"
                     /locus_tag="OO322_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5907139.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1308..2096)
                     /gene="phnC"
                     /locus_tag="OO322_002281"
     CDS             complement(1308..2096)
                     /gene="phnC"
                     /locus_tag="OO322_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5907140.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2229..2672)
                     /gene="yjdN"
                     /locus_tag="OO322_002282"
     CDS             complement(2229..2672)
                     /gene="yjdN"
                     /locus_tag="OO322_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5907141.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2830..3165)
                     /locus_tag="OO322_002283"
     CDS             complement(2830..3165)
                     /locus_tag="OO322_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5907142.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            3567..5795
                     /gene="crfC"
                     /locus_tag="OO322_002284"
     CDS             3567..5795
                     /gene="crfC"
                     /locus_tag="OO322_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5907143.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            5792..6670
                     /locus_tag="OO322_002285"
     CDS             5792..6670
                     /locus_tag="OO322_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5907144.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            6934..8436
                     /gene="proP"
                     /locus_tag="OO322_002286"
     CDS             6934..8436
                     /gene="proP"
                     /locus_tag="OO322_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5907145.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            8548..8637
                     /gene="pmrR"
                     /locus_tag="OO322_002287"
     CDS             8548..8637
                     /gene="pmrR"
                     /locus_tag="OO322_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5907146.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8613..9713)
                     /gene="pmrB"
                     /locus_tag="OO322_002288"
     CDS             complement(8613..9713)
                     /gene="pmrB"
                     /locus_tag="OO322_002288"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5907147.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9714..10382)
                     /gene="basR"
                     /locus_tag="OO322_002289"
     CDS             complement(9714..10382)
                     /gene="basR"
                     /locus_tag="OO322_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5907148.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(10379..12022)
                     /gene="eptA"
                     /locus_tag="OO322_002290"
     CDS             complement(10379..12022)
                     /gene="eptA"
                     /locus_tag="OO322_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5907149.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(12126..13463)
                     /gene="adiC"
                     /locus_tag="OO322_002291"
     CDS             complement(12126..13463)
                     /gene="adiC"
                     /locus_tag="OO322_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5907150.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(13600..14361)
                     /gene="adiY"
                     /locus_tag="OO322_002292"
     CDS             complement(13600..14361)
                     /gene="adiY"
                     /locus_tag="OO322_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5907151.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(14686..16953)
                     /gene="adiA"
                     /locus_tag="OO322_002293"
     CDS             complement(14686..16953)
                     /gene="adiA"
                     /locus_tag="OO322_002293"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5907152.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(17152..18060)
                     /gene="melR"
                     /locus_tag="OO322_002294"
     CDS             complement(17152..18060)
                     /gene="melR"
                     /locus_tag="OO322_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5907153.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            18343..19698
                     /gene="melA"
                     /locus_tag="OO322_002295"
     CDS             18343..19698
                     /gene="melA"
                     /locus_tag="OO322_002295"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5907154.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            19801..21222
                     /gene="melB"
                     /locus_tag="OO322_002296"
     CDS             19801..21222
                     /gene="melB"
                     /locus_tag="OO322_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5907155.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(21361..21990)
                     /locus_tag="OO322_002297"
     CDS             complement(21361..21990)
                     /locus_tag="OO322_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5907156.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(22113..23759)
                     /gene="fumB"
                     /locus_tag="OO322_002298"
     CDS             complement(22113..23759)
                     /gene="fumB"
                     /locus_tag="OO322_002298"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5907157.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(23837..25177)
                     /gene="dcuB"
                     /locus_tag="OO322_002299"
     CDS             complement(23837..25177)
                     /gene="dcuB"
                     /locus_tag="OO322_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5907158.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(25748..26467)
                     /gene="dcuR"
                     /locus_tag="OO322_002300"
     CDS             complement(25748..26467)
                     /gene="dcuR"
                     /locus_tag="OO322_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5907159.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(26464..28095)
                     /gene="dcuS"
                     /locus_tag="OO322_002301"
     CDS             complement(26464..28095)
                     /gene="dcuS"
                     /locus_tag="OO322_002301"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5907160.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
BASE COUNT         6670 a         6888 c         7338 g         7223 t
ORIGIN      
        1 gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag cggggccatt
       61 tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct cagaagcgag
      121 aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc cggtctgtag
      181 gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc ggatgcgacg
      241 ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga actcatcgcg
      301 cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc ggtggttttc
      361 gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat ctctttaaac
      421 agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa cggcgcccag
      481 cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat aaagaagtcg
      541 tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg atcgcctagg
      601 atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc ggaggttttc
      661 agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt ggcgacggcc
      721 agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc ggagatattg
      781 tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt aggatcgcca
      841 ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt gatcgggctg
      901 tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc gaccgtctgg
      961 gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt gccgtaccag
     1021 gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc cggggcaaag
     1081 aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg cgtccattgc
     1141 ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc cttttcctgc
     1201 tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt gaaggccagc
     1261 gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca ggcagctttc
     1321 gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc gttatcaaac
     1381 agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat gcgttcgcag
     1441 tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc gtcgttctgg
     1501 ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag cgaggcgatg
     1561 ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat cgccacacgt
     1621 tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac catgccaacg
     1681 cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct aaaacaggtg
     1741 cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac gctcaggcgg
     1801 ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt gcggatatcg
     1861 cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg gctaccggcg
     1921 gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt gccggaaccc
     1981 gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac cgcatgcagc
     2041 gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt ttgcatgatg
     2101 cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt ggttaattaa
     2161 aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag ggcgggcctc
     2221 ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg tacgccaaat
     2281 ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc catttcgatt
     2341 tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg ttgcgaatcg
     2401 agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc gctcatcatg
     2461 atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg aaattgcatt
     2521 ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc gaagctgatt
     2581 ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc ggaacagtta
     2641 ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa gagaagccag
     2701 ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc gcaagcgtcg
     2761 catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg cttaccgatc
     2821 acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac caaaaccgtc
     2881 gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca ctttggtgcc
     2941 aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg taacgctgtc
     3001 gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc tttcctgtgc
     3061 aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc cgttatcttc
     3121 gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga tttcctcaaa
     3181 agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg caattataca
     3241 caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt tttcatttga
     3301 aaggaaacgc aaaaagaaaa taatcttttt gcctctattt attatttaag ataatgttaa
     3361 tgctctactt gttctttttt tattcacatg atatttttat ctttctgacg ttttaaacgc
     3421 atcagaagac gttatttatt ctgataaaaa aacaacacac taaatataaa attatttctt
     3481 gcagtaatta taaaagccga aaacagaact caaaaacaat ctggctaaat aaaagtaaca
     3541 aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag ttaagtcagg
     3601 aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa aaaatgcctc
     3661 tctctgtacc cggagacgac gcgccacaac tggctttacc ctggagtcag cctaatatcg
     3721 ccgaacgtca cgcgatgctg aataatgagt tgtgtaaaat ttcccgactg gaaatggtgc
     3781 tggcaattgt cggtaccatg aaagcaggga aatcaaccac cattaatgcc attgttggta
     3841 cggaggttct gcctaatcgt aatcgcccaa tgactgcgct gccgacgctt attcgccata
     3901 cgcccgggca aaaggaaccg gtactgcatt tttcacatgt cgcgccaatc gattgtttaa
     3961 ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc gatgtgctgg
     4021 aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc gctttcgaaa
     4081 aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat gatttagtgc
     4141 gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc attgagcata
     4201 ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggaaagttat cccggtcagt
     4261 tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg caaaaaatgc
     4321 ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat acgcaactga
     4381 aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa tcggtgccgc
     4441 tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac gccgaccagg
     4501 tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag cagatattcc
     4561 cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg ctatgagtta gccaacaacg
     4621 gtaagttacc agcgccagag caacaacgct gggtggaaga ttttgcccat gccgcgctcg
     4681 gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca tattcgtcat gctgccgatc
     4741 agttgtggga agattcgctg ttcgcccagc caattcaggc gttgcttcat gccgcttacg
     4801 ctaacgcctc attgtatgct ctgcgatctg ctgcgcataa actgttgaat tacgcgcagc
     4861 aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt gaacaattgc
     4921 ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt gcaactcaat caggcgcagg
     4981 tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac cactttctgc
     5041 gtcaacagca agatgcggtg aatgcgcagt tagccgcctt gtttcaggat gattcggagc
     5101 cgttaagcga gattcgtacc cgctgtgaga cactgttaca gacggcgcag aacaccatca
     5161 gtcgcgactt tacgctgcgt tttgccgaga tggaatccac cctttgccgg gtgttaaccg
     5221 atgttattcg ccccattgag caacaagtca aaatggaatt gagcgagtca gggtttcgtc
     5281 ctgggtttca ttttcctgtt tttcacggcg tagttcccca cttcaacact cgccagctgt
     5341 tcagtgaagt catttcgcgc caggaagcaa cggacgagca gagcacgcgt ttaggcgttg
     5401 tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga aatgagaaat
     5461 ccccgacaga aacggttgat tacagtgtgt tgcaacgagc attaagcgca gaagtcgatc
     5521 tctattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct gttacggcag
     5581 gcatgaatac ttttttcgct gagttcgctt catgtttgac agaattacag acgcgtttac
     5641 gcgaaagtct ggctctgcgt caacaaaatg aatcggtggt caggctgatg cagcagcaat
     5701 tgcagcagac tgtgatgact cacagctgga tttacaccga cgctcagctg ttacgcgatg
     5761 atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg acggccccgg
     5821 tcgcgtgctg gagtcggttc atccgcgctt tttagtggat ctggcgcagg gcgatgatgc
     5881 ccgccatcct caagcccatc agcagcagtt tcgtgaacga ctgatgcagg aacttctttc
     5941 gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta aatgcgccgc ttagcctgcg
     6001 actgacaatg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg cgctgacgca
     6061 gatcgcgcag catctggcgc tgctgcaaaa aatggatcac cgccagcact ctgctttccc
     6121 ggagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt gtcgctggaa
     6181 ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggca ggtgatcaga gcgagcaaat
     6241 ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg ttgcctgggc gctgttttat
     6301 cgttctggag gagttgcgct ggggtgcatt tggcgatgcc tgccgtctgg gaagcccgca
     6361 agcggtggtg ttgttgctgg gtgatttgcg cgagaaagcg acacaacatc tggcagagag
     6421 tatcaatgcg gcaccgacta cgcgtcacta ttaccatcag tggtttgcct cttcgaccgt
     6481 tccgacgggc ggggatcatg ctgatttttt aagttggctg ggaaagtgga ccacggcaga
     6541 taaacaaccc gtttgctggt cagtgaccca acgctggcaa actgtcgcgc tggggatgcc
     6601 acgactctgt tcagcgcagc gtttggtggg ggcaatggtc gaggaaatct tctctgtaaa
     6661 tttggcgtaa ataatcagtt acatcaatga gtcctaaacg aaatccatgt gtgaagttga
     6721 tcacaaattt aaacactggt agggtaaaaa ggtcattaac tgcccaattc aggcgtcaac
     6781 tggtttgatt gtacattcct taaccggagg gtgtaagcaa acccgctacg cttgttacag
     6841 agattgcatc ctgcaattcc cgctcccctt ttgcggccgt cgcgctgatt tttctggcgt
     6901 ttgcggaaat gggccaactc tgcgaggaaa gctatgctga aaaggaaaaa agtaaaaccg
     6961 attacccttc gtgatgtcac cattattgat gacggtaaac tgcgtaaagc cattaccgca
     7021 gcatcactgg gtaatgcaat ggaatggttt gattttggtg tttatggttt tgttgcttac
     7081 gcattaggta aagttttttt cccgggggct gaccccagcg tgcagatggt tgctgcactt
     7141 gccactttct ccgttccctt tctgattcga ccgcttggcg gactcttctt tggtatgttg
     7201 ggcgataaat atggtcgcca gaagatcctc gctatcacta ttgtgattat gtcgatcagt
     7261 acgttctgta ttggcttaat accgtcctac gacacgattg gtatttgggc accgattctg
     7321 ctgttgatct gtaagatggc acaaggtttc tcggtcggtg gtgaatatac cggggcgtcg
     7381 atatttgttg cggaatactc ccctgaccgt aaacgtggct ttatgggcag ctggctggac
     7441 ttcggttcta ttgccgggtt tgtgctgggt gcgggcgtgg tggtgttaat ttcgaccatt
     7501 gtcggcgaag cgaacttcct cgactggggc tggcgtattc cgttctttat tgctctgccg
     7561 ttagggatta tcgggcttta cctgcgccat gcgctggaag aaactccggc gttccagcag
     7621 catgttgata aactggaaca gggcgaccgc gaaggtttgc aggatggccc gaaagtctcg
     7681 tttaaagaga ttgccactaa atactggcgc agcctgttga catgtattgg tctggtaatt
     7741 gccaccaacg tgacttacta catgttgctg acctatatgc cgagttattt gtcgcataac
     7801 ctgcattact ccgaagacca cggggtgctg attattatcg ccattatgat cggtatgctg
     7861 tttgtccagc cggtgatggg cttgctgagt gaccgttttg gccgtcgtcc gtttgtgcta
     7921 cttggtagtg ttgcactgtt tgtgttggcg atcccggcgt ttattctgat taacagtaac
     7981 gtcatcggcc tgatttttgc cgggttactg atgctggcgg tgatccttaa ctgctttatg
     8041 ggcgttatgg cttctacctt gccagcgatg ttcccgacgc atatccgtta cagcgcgctg
     8101 gcggcggcat ttaatatttc ggtgctggtt gccggtctga cgccaacact ggcggcctgg
     8161 ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt acctgatggt agtggcggtg
     8221 attggtttaa tcaccggcgt aaccatgaaa gagacggcaa atcgtccgtt gaaaggtgcg
     8281 acaccggcgg cgtcagatat acaggaagcg aaggaaattc tcgtcgagca ttacgataat
     8341 atcgagcaga aaatcgataa tattgaccac gagattgccg atttgcaggc gaaacgtacc
     8401 cgcctggtgc agcaacatcc gcgaattgat gaataagctg aaacggatgg cctgatgtga
     8461 cgctgtctta tcaggccaat tgaactctta aggttcactt aatctctgac gcgcatactc
     8521 tcctccaggt taacggagga gagtgcaatg aaaaaccgtg tttatgaaag tttaactacc
     8581 gtgttcagcg tgctggtggt cagcagcttt ctttatatct ggtttgccac gttctgatct
     8641 ttcttcagcc gtacccaggc ccgcgtgccg gaagtctctt gccggttttg caggaaaaac
     8701 tgcccgtgat gcaactgtgt aatgcggctg acaatactta accccagacc aatcccgcca
     8761 taacggctgt ccatacgtac aaacgcttta ctcaactccc cgcatttact ctcatcaata
     8821 cctggtcctt catcttcaac tgccatgacc gctccgccat cttcttgcag cttaatcata
     8881 atgttgctgc cttgcgggct gtaacgatgg gcgttttcta ccaggtttcg caataacatc
     8941 cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg cgctctctgg caatagcagg
     9001 gtttgctgtc gctggtcgag catggtactg agttcgtcat acgaggggag aatgacatct
     9061 tccagcagtt ttacatgttg ataattaccg gaagaaaatg actgtccggc acgcgccagt
     9121 tgcagcagct gggagacgct ctccatcatc tgatcaagcc gtgccactaa cggtgctaca
     9181 tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac gcacccccgc cagtggcgtt
     9241 cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt tatccagcgt gctggtcagg
     9301 cgactgacca gatcgttcaa cgccgaaacc accgcttcga tttcgagggt ggcgctgtga
     9361 atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca gctctttttg cagctccgcc
     9421 agcgggcggg tgatgcggcg taccgcctga tagcagataa atagcgtcag gctgaccata
     9481 aagacgccgg ggacaatcag gctggcgacc gcctcgcgga tctcacgcat gatgtggcga
     9541 tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct gaatctgctc ggtactttca
     9601 tgccatagcc agaagacgct gatcagctca aacaccaaca aaatggcccc gatggtcaat
     9661 atcagccgtt ggcgcagcga tataggtcgg cgcagaaaac gcatcagatt caattagttt
     9721 tcctcattcg cgaccagcat atagccaaag ccgcgcacgg tgcggatacg ggctttgccc
     9781 actttgtcgc gcagattgtg gatatgcact tccagggtgt tggtcgaggg ttcattgtcc
     9841 cagttataga tgtcgttgta gagaatttcc cgatgtaccg gactgcctgc tttgagcatt
     9901 aaccgtgaca gcagagcata ttctttgggc gtcagaatca actcttcacc gcccatccat
     9961 acctgacggc gacccatatt cagcgtcaga ttgccaacaa tcagctcact ttcgccctga
    10021 ttattatggc gtcgtagcag ggcgcggata cgggcgtgta actcttccag cgcaaaaggc
    10081 ttcaccagat agtcatcggc accgacatcc agcccggcga ttttgtcggt cagcgtatcg
    10141 cgagcggtga ggatcagtac cggcagggtg tattttttct gccggatacg ggcgagaaaa
    10201 tgcagtccat cttcgtcggg taaccctaaa tccagtacca ccaggctgta atgaccgtcc
    10261 tcaaggcttt gttccgccat ccgcgcggtt gtcacgccat cgcacgcgta gccttcggtt
    10321 tgcgccgcca gaatcagtcc ctgcaataac agcgtatcgt cttcaacaat cagaattttc
    10381 atttactcac tctcctgcaa gtttgcagaa tatcatccgc ggcctggtaa tacttcgtct
    10441 caacgccagt taatcccaat agcgtggaga ataaattgtc ttgtgaatag tgttgcgttt
    10501 gcgcctgttt ttgcaggcag ttctggtcaa cctgataccg cttttgataa tcctccgaca
    10561 gccacagcag cattggcacc tgtttttggc tatccggggc gatggcataa ggcagaccgt
    10621 gcagatagat gccattttca cctaacgatt caccgtggtc agaaagataa accaggctgg
    10681 tggtaaattt atcctgatgt tctttaagca gattaatcgc tttatcaaca atatagtcga
    10741 cgtaaaccag cgtgttgtcg taagtgttca ccagttgctc tttggtacag gtctggatct
    10801 cattggtgtc gcaggttggg gtaaatttcc tgaactgcgg cggatagcgg ttgtaatagg
    10861 tcggaccgtg gctgccgatg gtgtgtaaga caatcacgcc atcaccctgc aggttattga
    10921 tgtactcttc cagcccgtgg aacagcactt cgtcatagca ttcgccgttg atgcactgat
    10981 caggcagatt cagcgcggtg acgttctggt gaggcacgcg gtcgcaggca cctttacagc
    11041 cgccatcgtt gtcattccac agcacgttga tgcccgctcg ctgaatgata tccagcacgc
    11101 cttcctggtg ctgtgccagc tcttctttgt agtgctcacg cggcatatcc gagaacatgc
    11161 acggtactga aactgccgtt gccgtgccgc aagatgcggt attagggaaa tagaccacgt
    11221 tatctttcgc cagccgcggg ttagtttcac gcgggtagcc gttgagggag aagttctccg
    11281 cccgcgaggt ttcgccgaca atcaggatgg tcaaattttt acgtttttcg ttctgcatta
    11341 acgggttgcg gtgcgcgtct tcaccaattc gcaccagcgg cagatttgcc agtcgctgat
    11401 gggagtacca tgaccagctg gcaacaatgc tgttagaggg gcttaaggat ttcaccagct
    11461 ctttgttatt gcggaacaac gaggcgtagt ctttataaaa cagtgcggcg accagcaaaa
    11521 tcagcagtac agaaaccaga atattggctc cacggaaaag aacactgcgc agacgcgagg
    11581 tggcaggttt gatttttagc cagcaggcaa tcagctcagc aagcacgccg ctgaatccca
    11641 gcgttaataa catttgcggt gtcatcagcg cataactttc tgctggagtg gtatcaataa
    11701 tattggcaat catcgagcgg tcgatgacga tgccgtaagt cattatgaaa tattgtgcag
    11761 ccgcgccaat cagaataaac aggcaggcca gtggtcgatt aagccataag aaagagctta
    11821 gtgtcaggac aatattaatc acgctgaaag cgacaaccgg catcgacaag aaaaccagta
    11881 cgttatgcag cgaatccagc ggcagcgcct gcaacacctg tttaaaaaag gcaatattca
    11941 ggcagataga gatataaaaa gcggccaaca atagccaggc gagtaaattc aaagagggtc
    12001 tttttagtag gcgcttcaac atcacggtgt ttccatcgaa caaagtgcgc atatgctcgc
    12061 aaagcaaaat taagccaacc ttaagttctt aaggttggct tttatgtttg ctggattaag
    12121 gggaattaat ctttgcttat tggtgcatct aagggatacg ggtttttatg tagccggttg
    12181 taattcagcg catacatggc ggtgatgacc atcagggtga caaatgacca cataacctct
    12241 ttcgccccgg accccaccac ggcccaaatg cagtagagga aggcaatggt agtaacagcc
    12301 agatatgccg ggcgtgcttt accaaagtga ccgtggccga gcagcagtaa cgccgcacag
    12361 gtgtaaagat atggcaccag tgtaaagatg accgagacgg aagaaaccag accgaactct
    12421 ttggtcgcgt ttggtgaaat gctgctgagc tggaagatgg tcatcaaaat accgacgata
    12481 atcagccccg ccactggcgt acccgcttta tttacacggg caaaaatcgg tgggaacagt
    12541 ccgtcatcgg cagcggcttt cgccgtttga cccgccagca acgtccagcc gcccagtgaa
    12601 cctaagcaac ccgcagctgc gcagaaggaa acaatggccc cagcggtgtc acccaacgcc
    12661 atccgtgcgg catcaccgaa tggcgaggca gaaacgcgca gtgcggcatt agggatcatc
    12721 cccataatcg cggtggtgga gagcacatag caaacggcgg caatcaatac cccaccaatg
    12781 gtggcgatag ggacattgcg tttcgggttt ttcaccacac ctgcggcaac ggaggcactt
    12841 tccacaccga tgaacgacca cagcgtaacg ttaagggtac tttgaatcgc accgaaggtg
    12901 cccaggccgc tgacgttcca tgccgccata taggtttcac cacggaacca gaaccagcca
    12961 aatacggcaa tcccgacgat ggggatcagc gccagcacag tggcaacggc ctgcacgcgg
    13021 gtgatcattt tcggaccgac aatgttcagc aggacgaaga tccacagcac cacgacgcag
    13081 gtgatggtta ataccaatgg atctttcaga atcgggaaga agtaacttaa atatcctacg
    13141 ccaatgacca ccatggcgat attgccgatc cagcaggcca gccagtagag gacgttggtt
    13201 tgataaccga gaaacgggcc aaagcagcgg cgggcgtaag cgtaagaacc accaggactt
    13261 gggtcgagga acgacatttt ggcgtatacc atcgagagcc ccagcgcacc gataatcgtc
    13321 accaaccatc cataaatggc aatcccgcca gtggaggcca ggtttgcagg taacagaaaa
    13381 acacctgacc ccataatatt ccccgacacc atcagggtga cggggattaa gcccactttg
    13441 tgagcatcag catccgaaga cataattaaa ctcctgcgaa ggcgagcttc gtgacaataa
    13501 attacgtcat atcatacgcc tgcattcgat agttgaatta ttatcgaccg ggttatcgct
    13561 gatgccgtta ttaatatagc tcttcgttaa ggttaaaaat caggcgacag tgcgttctct
    13621 gtgctgacta acataatgca gcggcgtcat accgtagaag tctttaaaga cagaaataaa
    13681 gtacgacgta ctgttgtagc cgcaggactg tgatacctga gagatatttt taccgtccat
    13741 cattaattca tttacggcat aacgcatgcg gcaggtggtg attatttggc tataactggt
    13801 gttttcgctt ttcaactttt tctttaacag acttgggcta agacataaac aactggctac
    13861 catactaaga ttccagtctt tgtgaatatc gctttcaata atttgataaa cgctgtcact
    13921 tacacaatta cgtaacatat acatcattaa tgaaaggaat tttttactgt cgagaaaacg
    13981 ggatagcacc gtgaataata atgcgcgtgt tcgttccttt tctgactcag tttctgcggg
    14041 catcatgctg tgttgcgcgg caacgcgaaa gacgtctggc gtcaggcatg gcagggtgag
    14101 tatgggcgta gcgctacgtt gccatactgg tatttgcgag agatctttat tcagaaaccg
    14161 gaggtaatct ttgatgatgt cgtgactaat atgagcaacc aaagtgttgt taagagagga
    14221 gacgtcgata atattatttt cacaatttaa taacgccata tggttagctt taaggctaat
    14281 aggttctttc ccattcaccc ttatccagac atctttttca gtcaataaaa caatacaagg
    14341 ttggtcgctg caaatcctca tacatgtact cctgagtgcg aataaaagac cagttctcgc
    14401 taaagcaaag cgataccggg aaatggttat ataattgcat gatgaatata aacatgcata
    14461 aaaataacga gttttactaa tattacaaat ttttatagta atttctcatg tacatatttt
    14521 tttacaggat aaataacagg caaaaaaaag cgtggggtgg ggccgcgcaa aaagtatgac
    14581 atttttgttt gcagcaattt ttatgaaaac gttgccatac tcaaaaggcc taaggccagg
    14641 cggcgtaatg ttatttaaac aattacgcct tcagcggaat agtggttacg ctttcacgca
    14701 cataacgtgg taaataccgt caataatttc agtcccttca gtttcgtgtt caaatctagg
    14761 gaaatggtgg tcccaggatt gcagcgagcg taaataactt acttgcggac tgtttttatc
    14821 gccgaagttt tcaccagaca gcagcatcgg gattcctggc ggatacggga taactgagtt
    14881 tgccgcgatg cgtcctggca gattttcaat ggataccagt tcgacattgt tgtcgacaat
    14941 cgcgttgtac gcttcacgcg gggtgatttc cgccaccggc aggccggaat aggcttcgtt
    15001 caaccgtgcg ccagggttgt tttctttcag ccaggcaaac atggtgtcac ccagatcgtg
    15061 aatccccatg ttcgcgtaag tgtcaggata ttgttcaaca agttccggca tcacctgcgc
    15121 cagcggtgtg ttggcgtcat agtggtgttt gaaggagcaa agggtgttaa ccagagtccc
    15181 ccatttccca cgggttacgc ccatagagaa caggaacata atttggaagt cagtggtgcg
    15241 ggtaggcaca atgccgtggc gaccaagcca ggcagtgacc agcgccgccg gaacaccggt
    15301 ttcttccagt tcaccatctt cacccattcc cggagcaagg atgctgactt taattgggtc
    15361 gagcatactc cagttatccg gaatatcttt gaagccgtgc cagctttcgc ccggatgcat
    15421 tacccagcag tcctgaacgg tggtcagcag tttggttggt gcgtcagcaa agtcataggt
    15481 tttgccggtt tgtgggtcgg tgacgacttc tttgttccac ggtttgaaga accagctacc
    15541 gtcagcggtg aactctttat atagccgcgc catcgcctga cggaaatcaa ccgcttcgtc
    15601 aatcacttcc tgtgtcagtg acaggccgct gttgccgtcc atcatcgaca ccgccacgtc
    15661 gttggatgcg cagatggcat acagcgggga ggtggtggca tgcatcatgt aggcctggtt
    15721 gaagcgggag aagttaatcg ccccacgacc ttcacgtaca tgaatataag aagcctgtga
    15781 cagcgcattc agcagtttgt ggatggagtg ggtggcgaaa acggtaggac cgttgtgatc
    15841 gccaggttcg ccgcgcatgg catagtgatc ggcatagatc gggttgaaac gtgcatagcc
    15901 gtaccaggct tcgtcaaagt gcagacgatc ggaggttttt tccagcagat cctgcgcttc
    15961 tttagcgtta taacacacgc cgtcataggt gcagttggtt accacgcagt aagacggttt
    16021 ttgcccggct ttgtctttgg tcagcgggct ttcactgatt ttcttttgca aggtttcagg
    16081 ttgcatttcc tgcggataga ttggcccgat aatgccgtag cggttgcggc ttggcaccat
    16141 atagaccggt ttcgcgcctg tcagcatcaa accttgttcg atggatttat ggcagttacg
    16201 gtcaacgacc acgacatcgt tatcggtcat gcaagcctgc atgatggtgc ggttagagcc
    16261 ggaagtaccg acgactaccg accaggagcg atcggcacca aatacgcgtg cggcatattt
    16321 ttcgctttcg ccaaatgcgc cagtatggtc aagcaaagaa ccgagggaag ttcgttcgat
    16381 gcccatgtcg gtacggaaca gattttcacc atagtagtca tggtagaaac gtccggcggg
    16441 tgttttggta aaaccaacgc cgccctggtg gcctggcgct gcccaggaat attcatggat
    16501 gtcactatat ttcatcagcg cgctgaacag tggcggcaac agctgctggc ggtagcgggt
    16561 catcgcggca acggcgcgtc cggcgataaa gtcggcggta tcttccagaa tccaggcgaa
    16621 ttcatcgaca agctccagca gatcccggtc cattgcggcg agggcttttt cccgatcgcc
    16681 caacaggaag accggcacgt tttgttggcg ctcatgaagc ttaccgatca attgtctgac
    16741 gttttgatgt tcgtccggat gctccatttg atagctgaac atcaggcagt caatggcttc
    16801 gtttgaagag agaatggcaa aaccatcatc aaaggaggtg gatttaatca cggtaacatt
    16861 ttgctggctt aaagcatctg ccagacgctc aacggcgtta ccgacccagg tgtcttgatg
    16921 gagaaactcg ctttcaacaa ttaatacttt catcattgct tacccggtta tgaaggaatc
    16981 ttcgttgcgg gcaagtatct tccggccttt tcgggctgta aagcgcgtga aaaacaaaca
    17041 tttttgaaat atttagtttt ataaacaata cgttatgtga ttattttaaa ttattttcac
    17101 tgcttattat cgacggctaa actatttttt tggctgatac tgatatcgtc tttagccggg
    17161 aaacgtctgg cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca tgccgacata
    17221 tttgccgaac gtgctgtaaa aacgactact tgaacgaaag cctgccgtca gggcaatatc
    17281 gagaatactt ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc gcatcgcggt
    17341 aatgtactgt ttcatcgtca attgcatgac ccgctggaat atccccattg catagttggc
    17401 gttaagtttg acgtgctcag ccacatcgtt gatggtcagc gcctgatcat agttttcggc
    17461 aataaagccc agcatctggc taacataaaa ttgcgcatgg cgcgagacgc tgtttttgtg
    17521 tgtgcgcgag gttttattga ccagaatcgg ttcccagcta gagaggctaa atcgcttgag
    17581 catcaggcca atttcatcaa tggcgagctg gcgaatttgc tcattcggac tgtttaattc
    17641 ctgctgccag cggcgtactt caaacgggct aagttgctgt gtggcaagtg atttgatcac
    17701 catgccgtga gtgacgtggt taatcaggtc tttatccagc ggccaggaga gaaacagatg
    17761 catcggcaga ttaaaaatcg ccatgctctg acaggttccg gtatctgtta gttggtgcgg
    17821 tgtacaggcc cagaacagcg tgatatgacc ctgattgata ttcacttttt cattgttgat
    17881 caggtattcc acatcgccat cgaaaggcac attcacttcg acctgaccat gccagtggct
    17941 ggtgggcatg atatgcggtg cgcgaaactc aatctccatc cgctggtatt ccgaatacag
    18001 cgacagcggg ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat ctgtattcat
    18061 ggatggctct ctttcctaga atatcagaat tatggcagga gtgagggagg atgactgcga
    18121 gtgggagcac ggttttcacc ctcttcccag aggggcgagg ggactgtccg agcatcatga
    18181 ggccgaaaac tctacttttc aggtaattta ttcccataaa ctcagattta ctgctgcttc
    18241 acgcaggatc tgagtttatg ggaatgctca acctggaagc cggaggtttt ctgcagattc
    18301 gcctgccatg atgaagttat tcaagcaagc caggagatct gcatgatgtc tgcacccaaa
    18361 attacattta tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc
    18421 catcgcgagg cgctgaaaac ggcgcatatt gccctgatgg acattgaccc cacccgcctg
    18481 gaagagtcgc atattgtggt gcgtaagctg atggattcag caggggccag cggcaaaatc
    18541 acctgccaca cccaacagaa agaagcctta gaggatgccg attttgtcgt ggtggcattt
    18601 cagattggcg gttatgaacc ttgcacggtg actgatttcg aggtctgtaa gcggcatggt
    18661 ctggaacaaa ccattgccga tacgttgggg ccgggcggta ttatgcgcgc gctacgtacc
    18721 attccgcatc tgtggcaaat ttgcgagaac atgacggaag tctgccccga tgccaccatg
    18781 ctcaactatg ttaacccaat ggcgatgaat acctgggcga tgtatgcccg ctatccgtat
    18841 atcaaacagg tcgggctgtg ccattcggtg cagggaacgg cggaagagct ggcgcgtgac
    18901 ctcaatatcg acccggctac gctgcgttac cgttgcgcag gtatcaacca tatggcgttt
    18961 tacctggagc tggagcgcaa aaccgccgac ggcagttacg tgaatctcta cccggaactg
    19021 ctggcggctt atgaagcagg gcaggcaccg aagccaaata ttcatggtaa tactcgctgc
    19081 cagaatattg tgcgctacga aatgttcaaa aagctgggct acttcgtcac ggaatcatca
    19141 gaacattttg ccgaatatac gccgtggttt attaagctag gtcgtgagga tttgattgag
    19201 cgttataaag taccgctgga tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg
    19261 cataaagagc tggaggagta taaaaacgcc tcccggattg atattaaacc gtcacgggaa
    19321 tatgccagca caatcatgaa cgctatctgg actggcgagc cgagtgtgat ttacggcaac
    19381 gtccgtaacg atggtttgat tgataacctg ccacaaggat gttgcgtgga agtagcctgt
    19441 ctggttgatg ctaatggcat tcagccgacc aaagtcggta cgctaccttc gcatctggcc
    19501 gccctgatgc aaaccaacat caacgtacag acgctgctga ccgaagctat tcttacggaa
    19561 aatcgcgacc gtgtttacca cgccgcgatg atggacccgc atactgccgc cgtgctgggc
    19621 attgacgaaa tatatgctct tgttgacgac ctgattgccg cccacggcga ctggctgcca
    19681 agctggttgc accgttaaaa cgcgactaaa cgctactgcg ccgggggatt tattccggcg
    19741 cacacctctg acgataccaa taacagaagg cgggcgttgg taacagcgac ccgataccct
    19801 atgagcattt caatgactac aaaactcagt tatggatttg gagcgttcgg gaaggatttt
    19861 gcgatcggca ttgtgtatat gtacctcatg tattactaca ccgatgtcgt cgggctgtct
    19921 gtgggtttgg tcggtacttt gtttctggtg gcgaggatct gggatgctat taacgatccg
    19981 attatgggat ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa accctggatc
    20041 ctgatcggta cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc gcatctgttt
    20101 gaaggtacta ctcagattgt ctttgtttgc gtgacctaca tcctctgggg catgacttac
    20161 accattatgg atattccctt ctggtcgctg gttccaacca tcacgctcga taaacgtgaa
    20221 cgcgaacaac tggttcctta tccgcgtttt tttgccagtc tggcaggctt tgttacggca
    20281 ggtgtgacgc taccatttgt taattatgtc ggcggtggcg atcggggatt tggctttcag
    20341 atgttcactc tggtattgat cgcctttttt attgtttcaa ccatcatcac tctgcgcaat
    20401 gtgcatgaag tcttttcgtc agacaatcaa tcgtctgctg aaggaagcca tctgacactt
    20461 aaagccatcg ttgcgcttat ttataaaaac gatcagcttt catgcctctt gggtatggct
    20521 cttgcttata atgtagccag caacattatt accggatttg ctatctatta tttctcatat
    20581 gttatcggtg atgcggattt gttcccctat tatctgtcgt atgcgggagc tgctaacctg
    20641 gtgacgttag tattcttccc acgcttagtt aaatcattat cccgacgcat tttatgggcc
    20701 ggagcatcta ttcttccggt gttaagctgt ggtgttctcc tgttaatggc attaatgagc
    20761 tatcacaacg tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt
    20821 ttctgggtat tacaggtcat catggtggca gataccgttg attacggtga atataaactg
    20881 cacgtacgct gtgaaagcat cgcttactcc gtgcagacta tggtggtgaa gggcggttca
    20941 gcctttgcgg cttttttcat tgcggttgtg ttagggatga ttggctatgt accgaatgtt
    21001 gaacagtcta cgcaagccct attaggtatg cagtttatta tgattgctct accgactctg
    21061 tttttcatgg taacgctgat tctctacttc cgtttctatc gcctcaatgg cgacacgctg
    21121 cgcaggatcc agattcatct gctggataaa tatcgcaaag taccgcccga gcctgttcat
    21181 gctgatattc cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca atagctaacg
    21241 aaaatggcct gatgcgatat gtttatcagg ccacgtctta atgtaatgct ttggatttgc
    21301 aaattttgta gaccggataa ggaattcacg ccgcatccgg catcaacaaa gtgcacgttg
    21361 ttatccagtt atcaagccat agcgccgtag ctggcggcaa tgaaagcgag cgagcaatat
    21421 cactgtcgtt aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt cccactggtc
    21481 gccctgggta ccgagaaaat catccgctcc ctggcccatc gccagtgccg cccaccactc
    21541 gattaattca tacatcgcgc ttatcgccag cgccacgcaa cagaccagaa acgccaccat
    21601 tttacgtccg cgcacgtaca tcccgcgaac gagaatttct cgtgccacca gtgcaggcac
    21661 cagcccctgg aaaaagtgcc ccagcttgtc atacggatta cgactcaacc ccagccattc
    21721 ctgcacctca aaaccaacgg ggactttcgc gtaggtgtac tgcccgccta ccatcaggat
    21781 gatggcgtga aagaagatga gcgtatagag cagcagcgtt aacgggtaac gtctggcggt
    21841 ggcaagtagt agcggcacga cgataatcac cggtgtcact tccagcagcc aggtgagttt
    21901 gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccagtgtt
    21961 aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga
    22021 ctattcacca gcaagcatga aattacaatc gcaaaagagg tggcccaggg ggatcacctg
    22081 gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg
    22141 ttgacgattt gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa
    22201 tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc gacgcattcc
    22261 agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag
    22321 aagccgccgt gtttatgaca cgcgtcggta acctgctggc tgcggttgcc tttcgccagc
    22381 atgatcatgc tgccgccgtg ggattgcagc agatccacat aggagtccat acgacctgcg
    22441 gtggttgggc caagtgaacc tgatggataa ccggcagggg ttttcgccgg acccgcgtag
    22501 tagatcgggt gatctttgat gtactgcgga agttctttac cggcgtcaat cagctctttc
    22561 agcttggcgt gtgcaatatc tcgacccaca ataatggtgc cagtgagcga caaacgagtg
    22621 gaaaccggat attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcgacc
    22681 ttcaccgctt cgccttcacc ggcctgacgc agttcttgtg gaatgtactg acctagattg
    22741 tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca
    22801 gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata
    22861 acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg
    22921 gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc
    22981 ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta atttgacggt tttcaggttg
    23041 ttttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta
    23101 ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggagt cagcagggct
    23161 ttggtttcct ggtagagata cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg
    23221 aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttagtgccg
    23281 gtgttgacct ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag gttatcttcg
    23341 atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg
    23401 cgctggcctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg
    23461 cctttggcgg cgatttcgga gtttcttaag aattgcagtg ccacgtactt gtcgttttcg
    23521 ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg acgtagcatg
    23581 aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg ttccactttc
    23641 aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat
    23701 tcggtattgt ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata
    23761 gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc
    23821 ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc
    23881 acgatacgct cacaccaatc aaccccggca gaataaaact gtggttgatg acgaagcgac
    23941 cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg
    24001 gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc
    24061 cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact
    24121 tggaaaccag cagcagaaca atggcatacg cccacggata ctctttcacc atttcaccca
    24181 gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac
    24241 cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg
    24301 cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa
    24361 taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac
    24421 caagaagggc gactacagcg attgccccga ggaaaatcca cattgccagc cagttgcttt
    24481 tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca cggttttccg
    24541 gtacggagat gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc
    24601 tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcagat
    24661 cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcatcgca accagagaca
    24721 cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg
    24781 ccatcggacg ttccggacgg atgttgttct taatggcgac gtcgtagatg atcggcagaa
    24841 tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc acaaacggcg
    24901 cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga
    24961 catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca
    25021 gcatgacatc aactggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca
    25081 gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac
    25141 atattagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct
    25201 atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga
    25261 gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac agtgaatatt
    25321 ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaaa
    25381 actctctggc gatgggttta tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt
    25441 atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttata
    25501 gattaatctg atctacccat ttgtgggtaa aaatacacat aatgcgggtg acataatagt
    25561 taattaactt ttgttatcgt tttgaaatta aaaacaccgt tcatctgaag agatattaat
    25621 ttttaacgat gatggaggga taattatatt tgatctggca caagttttac tgattaacga
    25681 tgtaacttgt gccaggggta ttttgcatta cggtaataat tattacttac agataaccag
    25741 cgatccgtta ttggcaatat tgtttcagca gtgagtagtg ttctgcctga atacggtaac
    25801 ggtaaaccgg acgccccgtg acgccataat ggatactggt gaacaagatg tggcagttga
    25861 ccagccagat gaggtatttt cggcaggaaa cacgcgagat gttaacctcg ttagccagct
    25921 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg
    25981 tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt
    26041 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
    26101 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
    26161 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
    26221 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
    26281 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgcatcg ctattgaaga
    26341 taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt
    26401 gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
    26461 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
    26521 ccccggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
    26581 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
    26641 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg
    26701 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc
    26761 gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct
    26821 gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
    26881 aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
    26941 aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
    27001 cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc
    27061 acgaagtgcg tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc
    27121 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
    27181 ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc
    27241 gtcgcgtaac acttccgaga catctaccac ctgtgaccat gagtggctga gcgtcgacag
    27301 tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
    27361 cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc
    27421 ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt
    27481 cagtacctta accagaatgc aggtgccaat cagtccgacc agcatgccaa ataataccga
    27541 ccagataatg ctccagcgac tgtcattgat ctgttgggtc acgcggctta actcaaggcc
    27601 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
    27661 agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca gcgcattaag
    27721 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgtg agtagcgaag
    27781 actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
    27841 gatggcctgg atgccactct cctgcggttt tttctgcaag ccctgacgga tttccggcga
    27901 gtcggcgagg gtacgcgcca ctgccagtgc cttgttggcc agcccgtctc gcgtcatatc
    27961 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct
    28021 gaccattaag atcacagtgg tactcaattt catcggacgt ttgcgtaata tgtggtaggg
    28081 caatgaatgt ctcatcagct tccttgtgtg acaaatttc
//
LOCUS       DAJTRM010000052        28051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRM010000052 DAJTRM010000000
VERSION     DAJTRM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.052"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            546..1316
                     /locus_tag="OO322_002302"
     CDS             546..1316
                     /locus_tag="OO322_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5907161.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1307..1780
                     /locus_tag="OO322_002303"
     CDS             1307..1780
                     /locus_tag="OO322_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5907162.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1887..2426
                     /gene="dnaT"
                     /locus_tag="OO322_002304"
     CDS             1887..2426
                     /gene="dnaT"
                     /locus_tag="OO322_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5907163.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2429..3166
                     /gene="dnaC"
                     /locus_tag="OO322_002305"
     CDS             2429..3166
                     /gene="dnaC"
                     /locus_tag="OO322_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5907164.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3215..3709
                     /locus_tag="OO322_002306"
     CDS             3215..3709
                     /locus_tag="OO322_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5907165.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3949..6240
                     /gene="opgB"
                     /locus_tag="OO322_002307"
     CDS             3949..6240
                     /gene="opgB"
                     /locus_tag="OO322_002307"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5907166.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6378..7400)
                     /gene="lgoD"
                     /locus_tag="OO322_002308"
                     /pseudo
     CDS             complement(6378..7400)
                     /gene="lgoD"
                     /locus_tag="OO322_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7539..8453
                     /locus_tag="OO322_002309"
     CDS             7539..8453
                     /locus_tag="OO322_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5907167.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8668..10029
                     /locus_tag="OO322_002310"
     CDS             8668..10029
                     /locus_tag="OO322_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907168.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10078..11742)
                     /gene="tsr"
                     /locus_tag="OO322_002311"
     CDS             complement(10078..11742)
                     /gene="tsr"
                     /locus_tag="OO322_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5907169.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11861..12307)
                     /gene="hpaR"
                     /locus_tag="OO322_002312"
     CDS             complement(11861..12307)
                     /gene="hpaR"
                     /locus_tag="OO322_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5907170.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12579..13868
                     /locus_tag="OO322_002313"
     CDS             12579..13868
                     /locus_tag="OO322_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5907171.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13865..15331
                     /gene="hpaE"
                     /locus_tag="OO322_002314"
     CDS             13865..15331
                     /gene="hpaE"
                     /locus_tag="OO322_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5907172.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15333..16184
                     /gene="hpaD"
                     /locus_tag="OO322_002315"
     CDS             15333..16184
                     /gene="hpaD"
                     /locus_tag="OO322_002315"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5907173.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16194..16574
                     /locus_tag="OO322_002316"
     CDS             16194..16574
                     /locus_tag="OO322_002316"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5907174.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16642..17445
                     /gene="hpaH"
                     /locus_tag="OO322_002317"
     CDS             16642..17445
                     /gene="hpaH"
                     /locus_tag="OO322_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5907175.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17456..18259
                     /gene="hpaI"
                     /locus_tag="OO322_002318"
     CDS             17456..18259
                     /gene="hpaI"
                     /locus_tag="OO322_002318"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5907176.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18281..19636
                     /gene="hpaX"
                     /locus_tag="OO322_002319"
     CDS             18281..19636
                     /gene="hpaX"
                     /locus_tag="OO322_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5907177.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19646..20536
                     /gene="hpaA"
                     /locus_tag="OO322_002320"
     CDS             19646..20536
                     /gene="hpaA"
                     /locus_tag="OO322_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5907178.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20563..21500
                     /locus_tag="OO322_002321"
                     /pseudo
     CDS             20563..21500
                     /locus_tag="OO322_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21727..23285
                     /gene="hpaB"
                     /locus_tag="OO322_002322"
                     /pseudo
     CDS             21727..23285
                     /gene="hpaB"
                     /locus_tag="OO322_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23303..23815
                     /locus_tag="OO322_002323"
     CDS             23303..23815
                     /locus_tag="OO322_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5907179.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24208..26358
                     /gene="btsT"
                     /locus_tag="OO322_002324"
     CDS             24208..26358
                     /gene="btsT"
                     /locus_tag="OO322_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5907180.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26408..26611
                     /locus_tag="OO322_002325"
     CDS             26408..26611
                     /locus_tag="OO322_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5907181.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26622..27578
                     /gene="yjiA"
                     /locus_tag="OO322_002326"
     CDS             26622..27578
                     /gene="yjiA"
                     /locus_tag="OO322_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5907182.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27647..>28051)
                     /locus_tag="OO322_002327"
     CDS             complement(27647..>28051)
                     /locus_tag="OO322_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5907183.1"
                     /translation="EPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6832 a         7354 c         7078 g         6787 t
ORIGIN      
        1 attaatagat taagcgaata aattatcctt agcattcact ttcatatcag catactcctg
       61 ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa cctggtgaaa
      121 tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa tgaaaaacag
      181 aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat gcgggaatca
      241 cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac taaaaaacgc
      301 gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt atagtgataa
      361 tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac cgagcttgtc
      421 ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa caaccgtgct
      481 tcatgataca aatggattct cattatctga acaatacgca gcacgtctat gacaaagggc
      541 gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag tgtggattat
      601 ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca cgactgggtc
      661 gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata gtgctgacaa
      721 ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc ggcattaata
      781 tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat ctgctggatt
      841 acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca agatggctgg
      901 tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac ggtggctggg
      961 atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc cagctgctgg
     1021 cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc gccaccacca
     1081 tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt gcgatggccg
     1141 ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc gatatgttta
     1201 aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg acactggcta
     1261 cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg gtgtgatcga
     1321 atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg tcggctttgc
     1381 gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg gctcgatagg
     1441 tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt caacctttat
     1501 ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc tggcgcatcc
     1561 gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg cttataccgc
     1621 aatgatttcg tcggtaaaaa tcagccagtt aggttacagc gaaccgttga tgattaccct
     1681 gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg gtctttccat
     1741 tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg cttaagcaca
     1801 cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct cccttctcgt
     1861 tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg gacgtcgttg
     1921 gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa ggcggtgtgg
     1981 ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca cgcctggctg
     2041 aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct ctggacgatc
     2101 aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa ttcgccatgt
     2161 atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg ggcgtggcgc
     2221 taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg caggcggaag
     2281 gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg caaatcggtc
     2341 gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa cctgacagcc
     2401 aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga tgcaacgcct
     2461 gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag agctactggc
     2521 ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa atcgggcgat
     2581 gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga actgctcctt
     2641 tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag cgcgccagta
     2701 tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc caggaaccgg
     2761 caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta aatccgtatt
     2821 gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga atagcggtac
     2881 cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga tcgatgagat
     2941 cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg tcgatcgccg
     3001 ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag agatgaccaa
     3061 gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat gggtgatctt
     3121 caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc cgtttctggc
     3181 ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta aacatcttct
     3241 gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg cgtcctggaa
     3301 agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga cacaggaagg
     3361 cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccaag ccttaagcgc
     3421 agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa agctggcttc
     3481 ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc tgaacagtga
     3541 agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga aaacaaaaga
     3601 tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa aggtgaaaac
     3661 caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa ccgcgaaatg
     3721 ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg ttttccccct
     3781 gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc ctaacaacat
     3841 cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt gtgcttctca
     3901 aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccatt gtcagaacta
     3961 ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc gggacgtaac
     4021 acctggtggt ttgcagccac gttaacggtg ctggggctat ttgtcgtttt aaatatcacc
     4081 ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct ctatacctta
     4141 accaacagcc tgaccggtgc tggcgtcagc aaatacatcc tgccgggtat cggcattgtg
     4201 ctggggctga cggcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg tcgtcatcat
     4261 ccgcaccatt ttggttacag cctgctggcg ctcttactgg cgctgggttc ggtggacgcc
     4321 agcccggcat ttcgtcagat aacggaactg gtgaaatccc agtcacgcga tggcgacccg
     4381 gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa actcaacctg
     4441 gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaagc tttcccggat
     4501 ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca cacgcagcag
     4561 ctgccaggaa cggattacac gattgcgggc atggtggctt ctcagtgcgg cataccgctg
     4621 ttcgccccct ttgaaggcaa cgcctccgcc tctgtctcca gcttcttccc gcagaacatc
     4681 tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca gggcgcaaat
     4741 ctgcgttttg ccggtaaaga tgtgttcctg aaatcgcacg gcttcgacca cttatacggc
     4801 tcagaagagc tgaaaagcgt ggtagccgac ccgcactatc gcaacgactg gggattctac
     4861 gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg ctcaggtcag
     4921 cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt tatctcgcgc
     4981 acctgtaacc gcaaaaaata tgattttgac ggtaagccga atcagtcatt cagcgcggta
     5041 agttgcagcc aggagaatat cgcgacgttt atcaacaaaa tcaaagcgtc accgtggttt
     5101 aaagatactg tcatcgtcgt ctcttctgac catttagcga tgaacaacac ggcgtggaaa
     5161 tacctcaata agcaggatcg caataacctg ttttttgtca ttcgtggcga caagccgcag
     5221 caaaagacgc tggcagtgaa gcgtaacacg atggataacg gcgcgacggt gctggacatt
     5281 ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca gtcgatgtcg
     5341 gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccagatat catccgcctg
     5401 tggaaattcc ctaaagagat gaaagagttc accatcgacc agcagaaaaa catgattgcc
     5461 ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa
     5521 ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc agctggccga tttcgctcca
     5581 cgcgacaatt tcgtctgggt tgacagttgc tacaagatgg cacaactctg ggctccggaa
     5641 ctggcacttt ccaccgactg gtgtgtctcg caggggcagc ttggcggtca gcaaattgtt
     5701 cagcatgttg acaaaacaac atggaagagc aagacggcat ttaaagatac ggtcatcgac
     5761 atggcgcgtt acaaaggcaa tgtcgatacg ctgaagattg ttgataacga tattcgctac
     5821 aaagccgaca gtttcatctt taacgtcgcc ggtgcgccag aagaggtgaa acagtttagc
     5881 gggatttccc gtccggagtc gtggggccgc tggtccaacg cgcagctggg cgatgaagta
     5941 aaaatcgagt acaagcatcc gctgccgaag aaatttgacc tggtgattac cgccaaagca
     6001 tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga acaaactctt
     6061 gtgctgggca atgaagtgac caccaccaca ctgcatttcg ataacccaac cgatgccgac
     6121 acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat cctcggacac
     6181 tcgccgcgta agctcgggat cggcatggtg gaaattaaag tggtagaacg tgaagggtaa
     6241 ttcctgactg atgtgtaggt cgcatagggt gcgacctaca cgccttctcc ggcttacaac
     6301 acattattaa gaaacaaaaa aaacagcgcc caacacttcc ccggacgctg catggcaact
     6361 ttaataatct cgccttatca gaaagtaatt acacccttaa tcaactcacg attgttaatc
     6421 acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt taacatcatg
     6481 tcggcagtga ttttcccttc cgccatcagt cgaccgactt tagcgaaatc ttccggcgtg
     6541 gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc ggagaactgc
     6601 aactcacctt taaacagacc gacaaatacc accgtgccgc cgtgacgaat cagattcacg
     6661 gtattattca tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc cagcgaacca
     6721 ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag cacgggcaac
     6781 tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac caccacctgt
     6841 gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc cccgaccacc
     6901 agcacctgct cgccgggagc aatggctgcg cgacgcaccg catgagcgct aatagcgaaa
     6961 ggttcaatca atgcggccgc ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc
     7021 ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac tgaaattttt
     7081 tcacagcagt tggtacgccc gcttttacac gccgggcatt gctgacaggc aacataaggg
     7141 atcacagcaa cttgctgacc atttttaaga tcagcaatat ttttacccag cccaacaatc
     7201 tccccacata tttcatggcc taaaacccgt ggataactaa aaaatggttg atttccaccc
     7261 taggcatgaa tatcggtacc gcaaatcccg acagacttaa tttttattaa tgcttcattg
     7321 tcacccggaa taggtatctc gcgttgtttc cagactaatt ctttcggctg ctggcaaatt
     7381 aaaacattca tcgtagacat gattcggcta tccatttttt gttgccttag atatcaatga
     7441 aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga aaacacacgc ataaaaatgt
     7501 tttaaatcgg gttttaatgg actcacaagg aggaaaatat gagtcgttca caaaatttac
     7561 gccacaatgt gattaaccag gtcattgatg atatggcgcg gggtcatatc ccttccccac
     7621 ttccatcgca aagtgcgctg gcggaaatgt acaacatcag ccgcactaca gtgcgtcata
     7681 ttctcagcca attacgcgaa tgcggcgtct taacgcaggt tggaaacgac tatgttattg
     7741 cgcgtaaacc tgaccatgat gacggtttcg cctgtactac cgcttcaatg agtgaacaaa
     7801 acaaagtgtt tgagcaggct tttttcacga tgataaacca gcgtcagtta cgccctaggg
     7861 aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg gttgtaagag
     7921 aatatctttt aaaattcggc cgttataatc ttattcaaag cgaaaagcgc ggccagtgga
     7981 gcatgaagca attcgatcaa tcctacgccg agcagctgtt tgagctacgc gaaatgctcg
     8041 aaacacattc acttcagcac tttctcaacc tgcctgatca tgatccccgc tggctgcagg
     8101 cgaaaaccat gctagaacgt catcggctac tgcgtgacaa catcggcaat agctttcgca
     8161 tgttctcgca acttgacaga gattttcact ccttactgct ttcagctgcc gacaatatct
     8221 tttttgatca atcgcttgag atcatctcag tcatctttca ttttcactat caatgggacg
     8281 aaagcgatct taaacagcgc aatatcattg ccgtcgatga acatatgacc attctcagcg
     8341 ctctgatttg ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat ttgaattcag
     8401 ctaaacaatc aatgattcga tccattaatg agaacacgcg ttatgctcat taagtacgga
     8461 ttaaaaacag caaactatta gcgggtgcac ggttttatca aaagataaat accgggaaat
     8521 tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag
     8581 tcctaaaact ttaacctgcc gttaatgtca accttgcgta atgcacaatt acgtaaacaa
     8641 ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt
     8701 tcttcattta ctccatcttc gtcggcagat attcccgtgc caccagatgg attagttcaa
     8761 cgcagtaccc gaattaaacg cattcaaacc accgccatgt tgttattatt ttttgcggcg
     8821 gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa
     8881 ttgggattaa gtgccaccga aatcggcgct ttgctctccg tgttttcact cgcttacggc
     8941 attgcgcaac ttccttgcgg cccactattg gatcgtaaag gcccacgcct gatgctggga
     9001 ctggggatgt tcttctggtc actgttccag gccatgtctg gcatggtgca cagctttacg
     9061 cagttcgtgt tggtgcgtat cggtatgggg attggtgaag cgccgatgaa cccatgcggt
     9121 gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg gacgcccgat gggcttcttc
     9181 aacgcagctt ctaccattgg cgttgctgta agcccaccga ttctggcggc aatgatgctg
     9241 gtgatgggct ggcgcgggat gtttattacc attggtgtac tggggatttt tctcgccatc
     9301 ggctggtata tgctctatcg caaccgcgag cacgtagaac tgactgccgt tgaacaagct
     9361 tatctcaatg caggtagcgt caatgcccgc cgagatccgc tcaattttgc cgaatggcgc
     9421 agcctgttcc gtaaccgcac aatgtgggga atgatgctcg gattcagtgg catcaactac
     9481 actgcgtggc tgtatctggc ctggcttcct ggttacctgc aaacagccta taacctggat
     9541 ttaaaaagca cagggttgat ggcggctatc cctttcctgt ttggggctgc cgggatgctg
     9601 gtcaacggtt acgttactga ctggctggtc aaagggggaa tggctccgat taaaagccgt
     9661 aagatctgca ttattaccgg gatgttctgt tctgccgcct ttacgctgat agtaccacaa
     9721 gcgacaacat ccatgatggc ggttctgctg attggcatgg cactgttctg tattcacttt
     9781 gccggaacat cctgctgggg cttgatccac gtcgcagttg cttctcgcat gactgcgtcg
     9841 gtgggcagta tccagaactt tgccagcttc atctgcgcct ctttcgcgcc gatcattact
     9901 ggttttattg ttgatacaac ccactcattc cgtctggcac taatcatttg cggttgcgtc
     9961 accgcagcgg gagcactggc gtacatcttc ctggttcgcc agccgatcaa cgacccgcgt
    10021 aaagattaaa ctccctatgc ccgataacat tttgcttatc gggcattttt atggcgatta
    10081 aaacgtttcc cagttctccc cgctatctgc cactgccatt ttacgcggcg tagctggcgt
    10141 cacggttttt accacagccg atgtttcacg ctgctgttgc tgctgttgct gaatccggaa
    10201 cactgccaca gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg cggcagcggc
    10261 agattcttcc accagcgcgg cgttctgttg agttacccgg tccatctcag caaccgctaa
    10321 gccaacctga tcgataccac ggctctgctc atcagaagca gaagctattt cgcccataat
    10381 gtccgtcacg cgggtcacag cactgacaat ctccgccatt gtttccccgg cgctttcgac
    10441 cagcgtagag ccaacatcca ctttccccac cgagtcttca atcaggcttt taatttcacg
    10501 aaccgcctgg gcgctacgct gggccagatt acgcacttct cccgcaacca ccgcaaaacc
    10561 acgtccttgc tcacccgcac gcgctgcttc aaccgccgcg ttcaaagcca gaatattggt
    10621 ctggaaggta atgccgtcaa ttacgctgat aatatcggcg attttctgcg aactggtgga
    10681 gatatcgcgc atagtctgca ccacgttatc taccactttg ccgccgcgtt gcgccgtttc
    10741 agaagcactt aatgccagat ggctggcctg gcgcgcattc tcggcgttct gtttcaccgt
    10801 tgcggtcagt tgctccatgc tggctgccgt ctcttccagc gaagcggcct gttgctcggt
    10861 gcgcgaagag agatcattat tgccggtagc gatttcgctg gcaccgctat agatggcatt
    10921 ggccccgttg cgcacatcac cgacggtacg catcagctct ccctgcatat ggcgcaaact
    10981 ctctgccagt tgccccatct cattagagcc atccacctca atcggtttca ccagatcgcc
    11041 gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca gcgaggcttt
    11101 aataccgaac cagacggcga agatgaccgc cagtacgacg atcatcacgc ccaccagaat
    11161 ccacatcgcc tggctgtagg aggcattgtt atcgctgacg gcgatatcat agagccgatc
    11221 gttttgctcc atgtaagcca catactgctt ctcgaaaccg tcctgatatc cctgggtcgg
    11281 ctgatcaaag aactcgttga ttttgcctgc acctaacagt tggatcagct ccgccagcgc
    11341 attgtgataa atatcgtaat tacgtttgat ctctgccgct gcggcggtgc tctgacgcgg
    11401 gtcacgcggc aacgcttcgt aatccgccca gtttttttcc gcctgtttca gcgaaatact
    11461 ggcactctgc atcagctcag caacggttga accgctacca atattattct gatccatcat
    11521 gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga cccagctgcc
    11581 attcagcgtg ggttgctgct ggcgaatggt ttgtaaaaca gtgaaatttt ctttgtcatt
    11641 ctttaaggca ttaaaaaaca gaccgcctga tgtcagttgt aaaaggccaa aaacggccaa
    11701 aaccagcagt aagctggtca cgatcttgat acgttttaac atggtttctc tttcctgtgg
    11761 accgatgcaa gattttcggc ctggaaagca aaaactttat gaaaaggtgt aacccgccat
    11821 ccatggaaaa accaggcgct ggttatcaga tactaaaaag ttattcatta tcgcccggga
    11881 tgtcttcttg tcgggaattg cccagagcaa taaattcctc taacagatgg gttaactgtt
    11941 gcattttctc ggcagtaaac tgagcttcaa tctgccgata ggcttcttca atctgtgtct
    12001 gagcgcggtt atacagcgcc tgcccctctt tggtcagcga gatatagagc ttccgctgat
    12061 cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc catccgcgtg aggatcccgg
    12121 tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc gtgaaaatcc attgatgggc
    12181 tttccgccag aatacgcacg atgcgccact gttgctcagt caaattatgc cgtttaacga
    12241 ttgggcgaaa ataactcatc gccgcttctc gcgcctggag caacgcaatg gttagtgagt
    12301 cgtgcattat ctttcccctg aaaaattcaa aatcattaat atagaaacaa ttaatcatca
    12361 cgttaccggg actgagctgc ccccgcaatt cttcatttta tcaaaattga tgatatttta
    12421 ttaacttact gatatttaat attttattat ctttaaatgt aaatagtttg ttaattagat
    12481 cacatttata tcacttactt ttgccaaact acgcaatcgg gaataaaagt aatcattaac
    12541 atattaatga ttaagatgat cgaatccgag gagtggtaat gaaaggcact atcttcgccg
    12601 tagcgttgaa ccatcgcagc caacttgatg cctggcagga agcgttccag caatctccct
    12661 acaaagcccc gcctaaaact gcggtctggt ttattaaacc gcgcaatacg gtgattggtt
    12721 gcggtgaacc gattcccttt ccacagggtg aaaaagtact gagcggtgcg actgttgcgc
    12781 tgattgtggg gaaaacggcg acgaaagtac gtgaagaaga tgcggcagag tacatcgccg
    12841 gatatgcgct ggataacgac gtcagcctgc cggaagagag cttttaccgt ccggcaatca
    12901 aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac cgtggctctc agcaatgtcg
    12961 ataatctgac tatctatacc gagatcaacg ggcgtcctgc cgatcactgg aataccgccg
    13021 atttacaacg taacgccgca cagctgctga gcgccctgag cgaatttgcc acgctgaatc
    13081 caggcgatgc tattctgctc ggcacgccac aggcgcgcgt ggaaatacag ccaagcgatc
    13141 gcgtgcgtgt tctcgcagaa ggtttcccgc cgctggaaaa tccggtagtg gacgaacgtg
    13201 aagtgaccac gcgcaagagc ttcccaacgc tgccacaccc gcacggtacg ctgtttgccc
    13261 tcggcctgaa ctacgccgac cacgccagcg aactggaatt taagccaccg gaagagccgc
    13321 tggtgttcct gaaagcgccg aataccctca ctggcgacaa ccagacctcc gtgcgcccga
    13381 acaatattga atacatgcac tacgaagcgg agctggtggt agttattggc aagcaggcgc
    13441 gtaacgtcag cgaagccgat gccatggatt atgtcgcggg ctacaccgtg tgtaacgact
    13501 acgccattcg cgactatctg gaaaactact accgccctaa cctgcgggtc aaaagccgcg
    13561 acggactgac gccgatgctt tcaaccatcg tgccgaaaga ggcgatcccg gacccgcata
    13621 atctgaccct tcgcaccttc gtcaacggcg agttacgcca gcaaggcacc accgccgatc
    13681 tgatcttcag cgtgcccttc ctgatcgcct acttaagcga atttatgacc ctgaatccgg
    13741 gcgacatgat cgccaccggc acaccaaaag gcttatctga cgtggtgcct ggcgatgaag
    13801 tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg aattgtgagt gaggaaacag
    13861 cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat gttgcaggta acgactactt
    13921 ccagactacc aatccggcaa cgggtgaagt gctggcggat gtggcctctg gcggtgaagc
    13981 ggagatcaat caggcggtag cggcagcgaa agaggcgttc ccgaaatggg ctgatctgcc
    14041 gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat ctgatcgacc agaacgtgcc
    14101 tgagatcgcc gcgatggaaa ccgcggacac gggcctgccg atccatcaga ccaaaaatgt
    14161 gttgatccca cgcgcttctc acaactttga atttttcgcg gaagtctgcc agcagatgaa
    14221 cggcaagacc tatccggttg acgacaagat gctcaactac acgctggtgc aaccggtagg
    14281 cgtttgtgcg ctggcgtcgc cgtggaacgt gccgtttatg accgccacct ggaaggtcgc
    14341 gccgtgtctg gcgctgggca ataccgcggt gctgaagatg tcggaactct ccccgctgac
    14401 cgccgaccgc ctgggtgagc tggcgctgga agctggtatt ccggcgggcg tgctaaacgt
    14461 ggtacagggc tacggcgcaa ccgcaggcga tgcgctggtc cgtcatcatg acgtgcgtgc
    14521 cgtgtcgttc accggcggta ccgccaccgg gcgcaacatc atgaaaaacg ccgggctgaa
    14581 aaaatactcc atggagctgg gcggtaaatc accggtgctg atttttgaag atgccgatat
    14641 tgagcgtgcg ctggacgccg ccctgttcac catcttctca atcaacggcg aacgctgcac
    14701 cgccggttcg cgcatcttta ttcagcagag catctatcca gaattcgtta aacgctttgc
    14761 cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat ccgaataccc aggttagggc
    14821 gcttatcagt cagcaacact gggaaaaagt ctccggctat atccgtctcg gcattgaaga
    14881 aggcgcaacc ctgttggcgg gcggcccgga taaaccgtcc gacctgcctg cgcacctgaa
    14941 aggcggcaac ttcctgcgcc caacggtgct ggcggacgta gataaccgta tgcgcgttgc
    15001 ccaggaagag attttcgggc cggtcgcctg cctgctgccg tttaaagacg aagccgaagg
    15061 cttacgcctg gcaaacgacg tggagtacgg cctcgcgtcg tacatctgga cacaggatgt
    15121 cagcaaagtg ttacgcctgg cgcgtggcat tgaagcaggc atggtgttcg tcaacaccca
    15181 gaacgtgcgc gacctgcgcc agccatttgg cggcgtaaaa gcctccggca ccgggcgtga
    15241 aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag aacgtctgca tttccatggg
    15301 cgaccatcca attccgaaat ggggagtctg atatgggtaa gttagcgtta gccgccaaga
    15361 tcacgcacgt accgtcgatg tatctctctg aactaccagg aaaaaaccac ggttgtcgcc
    15421 agggtgcgat cgacgggcat aaagagatca gcaagcgttg ccgggaaatg ggcgtcgata
    15481 ccattatcgt tttcgatact cactggctgg tcaacagtgc ttatcacatc aactgtgcag
    15541 accattttga aggcgtctac accagcaacg agctgccgca ttttattcgc gacatgacct
    15601 acaactacga gggcaacccg gagttggggc agctaattgc cgaggaagcc ttaaagctcg
    15661 gcgtgcgggc aaaagcgcac aatattccca gcctgaaact ggaatacggc acgctggtac
    15721 cgatgcgcta catgaatgaa gacaagcact tcaaagtggt ctccatttcg gctttctgca
    15781 cggttcacga ttttgccgac agccgcaagc tgggcgaagc gattctgaaa gcgatcgaac
    15841 agtacgacgg caccgtggcg gtccttgcca gcggttcgtt atcgcaccgc tttattgacg
    15901 atcagcgtgc ggaagaaggg atgaacagct acacccgcga gttcgaccgc cagatggacg
    15961 agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga gttttgcaat atgctgccgg
    16021 agtacgccga ctactgctac ggcgaaggca atatgcacga cacggtgatg ctgctgggga
    16081 tgctcggctg ggataaatac gacggcaagg tggagtttat taccgagctg ttcccaagct
    16141 ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc gtaaggacgt tttatgccgc
    16201 actttatcgt ggaatgtact gaaaatattc gtgaagaagc ccgcttacct gagctgtttg
    16261 ccagtgttaa cactgcactg gcggcaacgg ggatttttcc gcttggggga atacgtagcc
    16321 gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa gcacgattac gcctttgtgc
    16381 atatgacgct aaaaatcggc agcggacgca gtctggaaag ccgtcaggaa gtgggcgaaa
    16441 tgctgtttga tctgattaaa acgcacttcg cctcactgat ggagagccgt tacctagcgc
    16501 tttcatttga gattgcagag ctgcacccga cgttgaattt caaacagaac aacgtacacg
    16561 cgttgtttaa ataaatttgc accagcttcc aggccggatg ataaggcgct gccgcatccg
    16621 gcaatgccac aggatatcgc tatgttcgat aaacacaccc acaccctgat cgcccagcgt
    16681 ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga tctcgctgga ttacccggag
    16741 atcaccatcg aagacgccta cgccgtgcag cgcgaatggg ttcgcctgaa aatcgccgaa
    16801 agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga aagcgatgca ggcaagctcg
    16861 cagatcagcg aaccggatta cggtgcactg ctggacgaca tgttcttcca cgatggcagc
    16921 gatatcccga ccgatcgctt tatcgtgccg cgcattgaag tggagctggc ttttgtgctg
    16981 gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg tttacaacgc cacggactat
    17041 gtgatcccgg cgctggagct gatcgacgct cgctgccaca acatcgatcc ggaaacccag
    17101 cgcccgcgca aagtattcga caccatttcc gataacgccg ccaacgccgg agtgatcctc
    17161 ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt ggatctccgc cctgatgtat
    17221 cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg tgctgaatca tccggcaaac
    17281 ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg tacaactgga aaccgggcaa
    17341 atcattctcg gtggttcgtt caccctcccg gttccggcgc gtaagggcga caccttccac
    17401 gtcgattacg gcaacatggg ctccattagc tgccgctttg tttaaggaga gaacgatgga
    17461 aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag attggattat ggctggggct
    17521 gagcagcagc tacagcgcgg agttactggc cggaccagga ttcgactggt tattgatcga
    17581 cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc caactacagg cgattgcgcc
    17641 ctaccccagc cagccagtgg tacgcccatc gtggaacgat ccggtgcaaa tcaaacaact
    17701 gctggacgta ggcacacaaa ccttactggt gccgatggta caaaacgccg acgaagcccg
    17761 taaagcggta cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg gcagtgcgct
    17821 ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg caaaaagcca acgatcaaat
    17881 gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag aacttaccgc agattctgga
    17941 cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat ctgagcgccg atatgggtta
    18001 tgccggtaat ccgcagcacc cggaagtaca ggccgccatt gagcaggcga tcgtgcagat
    18061 ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat gagcaactgg caaaacgcta
    18121 tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac accaccctgc tcgcccgcgc
    18181 cgccgaagcg ctggcagcac ggtttggtaa tagttcttct atttcgatgg cgaagcaaaa
    18241 caacaactct gtctactaaa cgtacgatgt ggagcgcatc atgacgacct ctaccctgca
    18301 atataaagat aataaaactg ttgaaattga acatcgtgtc attaataaat tatttcgtcg
    18361 cctaatcatt tttcttttta ttctgtttgt tttttcgttt cttgatcgca tcaatatagg
    18421 atttgccgga ctgacaatgg gcaaggatct tggcctgacg tccacaatgt ttggcctcgc
    18481 cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc cccagtaata tgatgttagc
    18541 gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg gtctggggaa ttgcttccac
    18601 ctgcacaatg tttgcaacaa gccccgaaac actctatcta ctgcgtatgc tggttggtat
    18661 tgctgaggcg ggttttttac ctggtatttt ggtttatctg acctggtggt tcccggcata
    18721 tcatcgggcc agagccaatg ccttatttat gatagcgatg ccagtcacta tgatgctggg
    18781 attgatcctt tcaggctaca ttctggcctt ggatggactg tggaatctaa aaggctggca
    18841 atggctcttt ttgctggaag gtctgccatc ggtcattctt ggtgtcgtca cctggtttta
    18901 tctcaacgac acaccagacc aggcgacctg gcttgatgaa aacgaaaaac aggctttaaa
    18961 aacgatgatt ctccgtgaac aagagagtgc atttgcccat gcatctgttc cacgctcaac
    19021 cttacgtgaa gtcctgaccc cagccatcat gctatataca cttgcttatt tttgcccgac
    19081 taatactctg agtgcgatta atatctggac gccgcaaatc ctacagagtt tcaatactgg
    19141 cagtagcaac atcatgattg gtttgctggc ggccatcccc caattctgca caatttttgg
    19201 catgatctgg tggagtcgtc gttccgaccg tcttaaagaa cgcaaaaagc atacgattct
    19261 tccctacctg tttgccgctg ccggatggtt actcgcttct gcgacagatc acagcctcat
    19321 tcaactgagc agcatcatta tggcctcaat gggatcattt accgcgatga ctatcttttg
    19381 gacaacaccg gatcaagtta taagcttgca atcgcgtgcc gttgcgatgg ctgtcatcaa
    19441 tgctgtcggc aatgtaggtt cagcagtcag tccattactt atcggtattt tgaaagatgc
    19501 aaccggcagt tttagctcag gtttgtggtt tgtagcagga ttgctggtac tgggcgcgtt
    19561 agtcatgacc cgaatcccta tggggagtcg ggaacactct ctttcatctg aacttatcgc
    19621 accaaaaccg caataaggag tcatgatgtc cagtgaagct atctttgaac atggttatga
    19681 aaatattgat ttggtccggg aatatgatgc ccgctacgct ggtgaagatg tgcattatga
    19741 aacatttgcc cggttagcga atttctttgg acgcgatatg cgccctcact ggcacgatag
    19801 atattttcaa ctccattatc tggtcactgg acgcattact ttacaactgg atgagcactt
    19861 ctattctgtg cgcgcaccgc tttttgtact aacaccacca tcggtgccac atactttctt
    19921 tactgatcct gatagcgatg ggcatgttct gaccgtacgt caggagttaa tttggcccct
    19981 ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg ctacaaggta tctgtctgtc
    20041 actggaggga atggaacata cactggcggc attaaatcat tattggccga taatatccct
    20101 ggagtttcaa caaaataagc aaggacggga aattctattg caatcactgg cgcaaagtat
    20161 ttttacaatc ctactgcgtg aagcaccacc aaatgatatt tcaacatgca gcgtgcgcgg
    20221 cgagatgtgt ttattccaga agtttaatcg gctgattgac gaaaattatc gccaacattt
    20281 aatggtccct gaatatgcaa caatgctcgg tatgagtgaa tcacggctta ccgagctgtg
    20341 ccgacgtttt gccaatcaat cgccgaagag attgattttc gaacgagtga gccgggaagc
    20401 cagacggctt ctgctctata gcgagcaaag catcaaccag atagcccttg atcttggcta
    20461 caaagatccc gcctatttcg cccgtttttt caaccgaatg atgggttgct caccaacaca
    20521 attccgtggt cgctgaccat ttatcaacct gagagactcg acatgaaaat atctttacgc
    20581 tcactatttg tggcgtgcct gattgcgcca gtggcattca cctacgcctc cgaaccgcaa
    20641 taaatgaaaa ttgtactact tggaacgaaa ggtggccctt ctttattaaa agctgaccgt
    20701 ttaccgatgg caacggcact tattattggc aataaaacct ggttaattga tgctggttac
    20761 ggggccagct tacaactggt aaaaaataat attccactgc gccagattga taatatttta
    20821 ctcacccatt tacacagtga tcatattctg gactatccat cagtcttaat gaatgcatgg
    20881 gctagcggtc tgcaggacaa aactattcat gtattcggtc cgccaggcac ccgagccatg
    20941 accgctgcca gttggcaggt cttcgatcgg gacatcacac tgcgtatgga agaagaagga
    21001 aaacccgatc ctcgcaaact cgttacagct caagatgttg gtgaaggcgt agtttataaa
    21061 gatgataccg tgaccgtgac tgcattaaag gtccctcatt ctcctttccc tgacggtgag
    21121 gcttttgctt atcgcttcga cacacaaggc aagcgaattg tcttctcagg agatacctcg
    21181 tactttcccc cactggcaac tttctcagaa ggcgctgata tacttgtgca tgaggccgta
    21241 catgagccct ccgtcgctaa gctggcagac agtattggca atggtaaaac actggcaaaa
    21301 gccattgctt ctcaccatac gactattgag gatgtaggca aaattgccaa tgaagcaaaa
    21361 gtgaaaaaac tcatccttag ccacctggta ccaatcaccg ttccggatga aacatggaag
    21421 caagaagcac agaaaactat tcaggtccta tcgtcgttgg tcatgaaaat atgacgatta
    21481 ccattccatt cgccccataa aaaattccgt ccccgttagc ccttgttagc ggggaatatt
    21541 atgatttgcc ttactgtaag ttgatcacga tcgcagttta ctatttaact ccaccttgct
    21601 gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg acaaccgcgt tcaacgcttc
    21661 aattaaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa tactgtagag
    21721 gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtcctttcac cggggaagag
    21781 tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg agtgaaagac
    21841 gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact gtacgacgcg
    21901 ctgcacaaac cggagatgca ggactctctg tgttggaaca ccgacaccgg cagcggcggc
    21961 tatacccata aattcttccg cgtggcgaaa agtgccgacg acctgcgcca gcaacgcgac
    22021 gccatcgctg aatggtcacg cctgagctat ggctggatgg gccgtacccc agactacaaa
    22081 gccgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt cgagcagaac
    22141 gcccgtaact ggtatacccg tattcaggaa actggcctct actttaacca cgcgattgtt
    22201 aacccaccga tcgtcatttg ccgaccgata aagtgaaaga cgtttacatc aagctggaaa
    22261 aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc aactcggcgc
    22321 tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa aacccggact
    22381 tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt gaaattaatc tcccgcgcct
    22441 cttatgagat ggtcgcgggt gctaccggct cgccatacga ctacccgctc tccagccgct
    22501 tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact gatcccatgg gaaaacgtgc
    22561 tgatctaccg cgattttgat cgctgccgtc gctggacgat ggaaggcggt ttcgcccgta
    22621 tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact cgacttcatt acggcactgc
    22681 tgaaaaaatc actcgaatgt accggcaccc tggagttccg tggtgtgcag gccgatctcg
    22741 gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg tgttcagaag
    22801 cgacgccgtg ggtcaacggg gcttatttac cggatcatgc cgcactgcaa acctatcgcg
    22861 tactggcacc aatggcctac gcgaagatca aaaacattat agaacgcaac gttaccagtg
    22921 gcctgatcta cctcccttcc agtgcccgtg acctgaacaa tccgcagatc gaccagtatc
    22981 tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt ccagcgcatc aagatcctca
    23041 aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg tcacgagctg tatgaaatca
    23101 actactccgg tagccaggat gagattcgcc tgcaatgtct gcgccaggca caaagctccg
    23161 gcaatatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac gaccagaacg
    23221 gctggactgt gccgcacctg cacaacaacg acgatatcaa catgctggat aagctgctga
    23281 aataacgcag caggaggtta agatgcaatt agatgaacaa cgcctgcgct ttcatgacgc
    23341 gatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg ccggacaatg
    23401 cgggattacg gcaacggctg tctgctcggt cacggataca ccaccgtcgc tgatggtgtg
    23461 cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggcaagt tgtgcgtcaa
    23521 cgtcctcaac catgagcagg aactgatggc gcgccacttc gcgggcatga caggcatggc
    23581 gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc agccggtgct
    23641 aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat gtgcaggcga ttggcacaca
    23701 tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc acggacttat
    23761 ttactttaaa cgccgtttcc atccggtgat gctggaaatg gaagctgcga tttaagctcc
    23821 agccccttca cgcattcgtc gaaggggcat ttttattgtg atctcaatca catatctacc
    23881 cgatgaataa ttcttgccca accgctcacc gccattcctg accgcttaca tccctaaaat
    23941 aaccactcag ttatttacct tactttacgc gcacgtatct ctggcaacat cactgcagga
    24001 tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg gcctatgatt
    24061 aacaaattac tctatggatt ttggtttgca ggaaggcagc aagtgagtga atccccggga
    24121 gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg ccgcacctgt aagccaaaag
    24181 acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa gcacataccc
    24241 tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg
    24301 gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat
    24361 cgctactaca gtctgtacat cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg
    24421 cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta cgtgttgttt
    24481 ggtcaccact tcgccgctat cgccggtgct ggtccgctgg tgggaccggt tctcgccgcg
    24541 cagatgggct acctgcctgg cacgctgtgg ctgctggcgg gggtagtgct ggccggtgcg
    24601 gtacaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc tctcggtgag
    24661 atgatcaaag aagagatggg gccagtaccg gggactatcg cgctgtttgg ctgtttctta
    24721 atcatgatca tcatcctcgc cgttctggcg ctgattgtgg ttaaagccct ggccgaaagt
    24781 ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat gggtatctac
    24841 atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg ttattggtat cgtgctgctg
    24901 gttgcctcta tctacttcgg tggcgtgatt gctcacgatc cgtactgggg tccggcactg
    24961 acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt ttccgcactg
    25021 ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct gaaaatcggc
    25081 gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcg
    25141 atgacccagt acattgacgg tactggcccg ctgtggaaag gcgctctgtt cccgttcctg
    25201 ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc ttccggtacg
    25261 acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca ttggctacgg cgcaatgctg
    25321 atggagtcct tcgtggcgat tatggcgctg gttgcagcgt ccatcatcga accgggtctt
    25381 tacttcgcga tgaacacccc gcctgctggc cttggcatca ccatgcctaa cctgcatgaa
    25441 atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac cgcacacgcg
    25501 gcagcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct gcaaaccgcg
    25561 aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccgac gctggcggta
    25621 ggtatcgctc acgtgttcca caaagtgctg ccgatggctg acatgggctt ctggtatcac
    25681 ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg tacccgttct
    25741 ggccgcttta tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac
    25801 tctctggttg ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg
    25861 tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct gttcggtatc
    25921 tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct gattaagatg
    25981 aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct tatctgcacc
    26041 acctgggcgc tgggtctgaa actgttcagc accaacccgc agatggaagg cttcttctac
    26101 atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc gcagcagatt
    26161 gccaacatga accacatcgt tgtgaacaac tacaccaacg caggcctgag tattctgttc
    26221 ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggcttgc ggtgcgtaac
    26281 agcgacaaac gtactgacaa agaaacaccg tacgttccaa tcccggaagg cggcgtgaag
    26341 atctcttcgc accactaacc gtgtttagcc ccgcttcggc ggggctttgt tcttatcaga
    26401 gtgaactatg tttggtaact taggacaggc aaaaaaatat ctcggccagg cggcgaagat
    26461 gttgattggt attccagact acgacaacta cgtcgagcat atgaagacca accatcccga
    26521 caagccgtac atgagctatg aagaattctt ccgcgaacgc cagaacgcgc gctacggcgg
    26581 cgatggtaaa ggcggtatgc gctgttgtta atggagagag catgaacccg attgcagtta
    26641 ccctactcac cggtttttta ggcgcgggca aaactaccct gctgcgccat attcttaacg
    26701 aacaacacgg ctacaagatt gccgtgattg aaaacgaatt cggcgaagtc tccgttgatg
    26761 accaactgat tggcgaccgc gccacgcaga tcaaaacgct gaccaacggc tgcatctgct
    26821 gttcgcgctc caacgagctg gaggacgcac tactcgacct gctggataat ctcgacaagg
    26881 gcaatattca gttcgatcgt ctggtgattg aatgcaccgg catggccgat cccggcccga
    26941 ttattcagac ctttttctcc catgagattt tatgccagct ctacctgctg gacggcgtga
    27001 ttgcgctggt ggatgcggta catgccgatg agcagatgaa ccagttcacc atcgcccagt
    27061 cccaggtggg ctacgccgac cgtattctgc tgactaaaac cgacgtcgca ggcgaagcag
    27121 agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc accggtctac accgtcaccc
    27181 acggcgacat cgacctgggc ctgctgttca acaccaacgg ttttatgctg gaagagaacg
    27241 tcgtcagcac caaaccgcgt ttccacttta tcgcggataa acaaaacgat atttcgtcaa
    27301 ttgtggtgga actggattac ccggtagata tcagcgaagt ttcccgcgtg atggaaaacc
    27361 tgctgctgga atcggcggat aaactgctgc gttacaaagg gatgctgtgg attgacggcg
    27421 aacctaaccg cctgctgttc cagggcgtcc agcgcctcta cagcgccgac tgggacaggc
    27481 cgtggggcga tgaaaaaccg catagcacaa tggtgtttat cgggattcag ttgccggagg
    27541 aagagattcg ggcggcgttt gcgggattga ggaagtaagt tcgggaaaga tcaacttttt
    27601 gagagttcca ccgcaacatt attacgagtg gaactcttta gatatgtcag accaaccaaa
    27661 gcttcttcaa ttgcgttttc attttttcat gtctcatttt cagttcagcc aatccccatt
    27721 ctgaagaatt acaaacatct tttgttaaat aaaaagagct tttactatta agtttctcat
    27781 aaatagattt ctttttgata aaatcatagt gttgtgtttc agaatttttg gaaccactta
    27841 tgagtgttaa attacctatt ttatgcagcc agtagacatg ctcttgaggt tggaatctat
    27901 taatccagta ttcattaact aatgcttgag gaagaatatg ctcaattgtg attctccccg
    27961 tataggtttt aatcacgctc tcatcttgtt gctccatatc taagcgaagc aaaattgctt
    28021 ttattaagtt cacttgattc ggacgaggct c
//
LOCUS       DAJTRM010000053        27496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRM010000053 DAJTRM010000000
VERSION     DAJTRM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.053"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO322_002328"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO322_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5907184.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO322_002329"
     CDS             complement(1614..1937)
                     /locus_tag="OO322_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5907185.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO322_002330"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO322_002330"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5907186.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO322_002331"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO322_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5907187.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO322_002332"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO322_002332"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5907188.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO322_002333"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO322_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5907189.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO322_002334"
     CDS             7537..8574
                     /locus_tag="OO322_002334"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5907190.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO322_002335"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO322_002335"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5907191.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO322_002336"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO322_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5907192.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO322_002337"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO322_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5907193.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO322_002338"
     CDS             10745..11626
                     /locus_tag="OO322_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5907194.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO322_002339"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO322_002339"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5907195.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO322_002340"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO322_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5907196.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO322_002341"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO322_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO322_002342"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO322_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5907197.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO322_002343"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO322_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5907198.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO322_002344"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO322_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5907199.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO322_002345"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO322_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5907200.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO322_002346"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO322_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5907201.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO322_002347"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO322_002347"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5907202.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO322_002348"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO322_002348"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5907203.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO322_002349"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO322_002349"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5907204.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO322_002350"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO322_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5907205.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO322_002351"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO322_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5907206.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO322_002352"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO322_002352"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5907207.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO322_002353"
     CDS             24893..25273
                     /locus_tag="OO322_002353"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5907208.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO322_002354"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO322_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5907209.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO322_002355"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO322_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5907210.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27496
                     /locus_tag="OO322_002356"
                     /pseudo
     CDS             26442..27496
                     /locus_tag="OO322_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6776 a         6845 c         7210 g         6665 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactg
//
LOCUS       DAJTRM010000054        26132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRM010000054 DAJTRM010000000
VERSION     DAJTRM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.054"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(57..197)
                     /locus_tag="OO322_002357"
     CDS             complement(57..197)
                     /locus_tag="OO322_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5907211.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            439..732
                     /locus_tag="OO322_002358"
     CDS             439..732
                     /locus_tag="OO322_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5907212.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            804..1463
                     /locus_tag="OO322_002359"
     CDS             804..1463
                     /locus_tag="OO322_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5907213.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1555..2001
                     /locus_tag="OO322_002360"
     CDS             1555..2001
                     /locus_tag="OO322_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5907214.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2208..3108)
                     /gene="hlyE"
                     /locus_tag="OO322_002361"
                     /pseudo
     CDS             complement(2208..3108)
                     /gene="hlyE"
                     /locus_tag="OO322_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3481..3900
                     /locus_tag="OO322_002362"
     CDS             3481..3900
                     /locus_tag="OO322_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5907215.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3900..5168
                     /gene="umuC"
                     /locus_tag="OO322_002363"
     CDS             3900..5168
                     /gene="umuC"
                     /locus_tag="OO322_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5907216.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5214..5744)
                     /gene="dsbB"
                     /locus_tag="OO322_002364"
     CDS             complement(5214..5744)
                     /gene="dsbB"
                     /locus_tag="OO322_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5907217.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5890..7431)
                     /gene="nhaB"
                     /locus_tag="OO322_002365"
     CDS             complement(5890..7431)
                     /gene="nhaB"
                     /locus_tag="OO322_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5907218.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7652..8371
                     /gene="fadR"
                     /locus_tag="OO322_002366"
     CDS             7652..8371
                     /gene="fadR"
                     /locus_tag="OO322_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5907219.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8423..9955)
                     /locus_tag="OO322_002367"
     CDS             complement(8423..9955)
                     /locus_tag="OO322_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5907220.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10285..11583
                     /gene="dadA"
                     /locus_tag="OO322_002368"
     CDS             10285..11583
                     /gene="dadA"
                     /locus_tag="OO322_002368"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5907221.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11593..12663
                     /gene="dadX"
                     /locus_tag="OO322_002369"
     CDS             11593..12663
                     /gene="dadX"
                     /locus_tag="OO322_002369"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5907222.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12831..14245)
                     /locus_tag="OO322_002370"
                     /pseudo
     CDS             complement(12831..14245)
                     /locus_tag="OO322_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14856..16592)
                     /locus_tag="OO322_002371"
     CDS             complement(14856..16592)
                     /locus_tag="OO322_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5907223.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16687..17601)
                     /gene="ldcA"
                     /locus_tag="OO322_002372"
     CDS             complement(16687..17601)
                     /gene="ldcA"
                     /locus_tag="OO322_002372"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5907224.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17701..18312
                     /gene="emtA"
                     /locus_tag="OO322_002373"
     CDS             17701..18312
                     /gene="emtA"
                     /locus_tag="OO322_002373"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5907225.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18314..19048)
                     /locus_tag="OO322_002374"
     CDS             complement(18314..19048)
                     /locus_tag="OO322_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5907226.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19249..19503
                     /locus_tag="OO322_002375"
     CDS             19249..19503
                     /locus_tag="OO322_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5907227.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19553..21013)
                     /locus_tag="OO322_002376"
     CDS             complement(19553..21013)
                     /locus_tag="OO322_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5907228.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20950..21507)
                     /locus_tag="OO322_002377"
     CDS             complement(20950..21507)
                     /locus_tag="OO322_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5907229.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21533..22387)
                     /locus_tag="OO322_002378"
     CDS             complement(21533..22387)
                     /locus_tag="OO322_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5907230.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22387..23196)
                     /locus_tag="OO322_002379"
     CDS             complement(22387..23196)
                     /locus_tag="OO322_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5907231.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23206..23964)
                     /locus_tag="OO322_002380"
     CDS             complement(23206..23964)
                     /locus_tag="OO322_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5907232.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23961..24941)
                     /locus_tag="OO322_002381"
     CDS             complement(23961..24941)
                     /locus_tag="OO322_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5907233.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24941..25963)
                     /locus_tag="OO322_002382"
     CDS             complement(24941..25963)
                     /locus_tag="OO322_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5907234.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6527 a         6417 c         6772 g         6416 t
ORIGIN      
        1 cggcacagtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag
       61 gcctgtgtcc atattacgtg ggtaggatca ctatggcctt tgctgaactc aacattgaca
      121 ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac agccttactg
      181 atgctcttga ttttcattgg tagtctcctg ttcagaatga gtacgatgaa ctgttataat
      241 ataacaatcc cttacgggag aattccagtg gcttttctgc gttcggtaaa atcccggcgc
      301 ggcatttttt gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat
      361 ctacagctgg ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc
      421 atcttaaaga gtaaaagtat gttttgtgcg atttatcgaa gcagcaagcg tgaccagacc
      481 tatttatatg tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt
      541 tttggtcagc ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat
      601 gctgatattg aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg
      661 ccaccacccg aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac
      721 actaacaaat aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc
      781 cacgataagg tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg
      841 attatccggt gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga
      901 tgggcagcgc agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg
      961 atctgcggca gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac
     1021 tggcggtgtt gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca
     1081 ttgccggtta cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga
     1141 aagccgggca gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt
     1201 ttatccccgc ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa
     1261 acggcgaaaa acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga
     1321 tcgcttatat gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc
     1381 ctgatggcgt cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt
     1441 ctttgacaac tcgcgttttg taatactttt tgccgccaga aagcggcggc aaaacttgca
     1501 tcgctgtgcc agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc
     1561 gatgtacctt tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg
     1621 ttgtgtgatg gttgcggtca gtgttgcttg cataaactga tggatgaaga caccgacgaa
     1681 atctacttca ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac
     1741 tacgaacgtc gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca
     1801 acattcgaat ggctgccaat gacctgcgct tatcggttgc tggcggaagg taaagattta
     1861 cctgcgtggc atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct
     1921 gtgcgtcata tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat
     1981 aaacctgact gggcgcagtg ataaatttaa tagatgctga tgggggcttt cttaatatac
     2041 gtctggatgc tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt
     2101 acccgatacc ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa
     2161 atgtgataaa ccttatacct tgagtacatg gagagaataa tcgcttatca gacttcaggt
     2221 acttcaaaga gtgtcttttt accgtgtctt ttctgatact cattacaggt gttaatcatt
     2281 tttttggccg ctgcttttag caaagaaagc attaaatcat cataatcaac atagaatctg
     2341 gttgtttcag tttccgtttt tatctccccg atggcggcta tttcggtggt taatttcaat
     2401 ttggcggcat cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa
     2461 ctctgcacag attttaactt gttcttcaat tctggaatca gtttcccttc aactacgccc
     2521 gcagcaatag aataggaaat gattaaacca aatggaccgg cgacgacatc ggctgcggca
     2581 ccggcatacg cttccttcct gattttatct acctgtgact ggaaatagct gcttttttct
     2641 gaaaaatcat tggttaactg gctatctaac gccagcagtt tcccggaagc gttgttgaaa
     2701 ctttgtgagc ttaccagcag ggatttttgc gcttcattca gcttcgtgat gccgtcatcc
     2761 agtaccttaa tgaggggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata
     2821 cgctgcgagc aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa
     2881 atacttatcc tggctatcca taagtaaggt tttaatatcg ccgactaaaa cggaggctgc
     2941 ctgtgaatac tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca
     3001 ggggatgacc tgatcgagat atttattata aagatctaat gctccatctg cggtttcgat
     3061 tgcgttttta actacttcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct
     3121 ctttaaatat ataaattgta atgaaactcc tgttttacaa ctattaataa attttacttc
     3181 atctaattca tagttagccg ggcgggatgc gtcaatgtct ttatttctat taatatgata
     3241 aatatcaaac aatgtttaat gtcattatgg cgaatgcttc tattctattt ttttgccggg
     3301 tgatattttt catttctgct ggatgagcgt cgtcgccaga aggccacgtg agcacaggat
     3361 aagagaacga aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt
     3421 gatctgctgg caagaacaga ccactgtata taaaaacagt ataacttcag gcagattatt
     3481 atgttgttta tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat
     3541 cttgttcagt gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg
     3601 aatcaactgt tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct
     3661 atgattgatg gtggaattag tgacggtgat ttactgattg tcgatagtgc tattaccgcc
     3721 agccatggtg atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa
     3781 ctacgcccga cggtacagct tattcccatg aacagtgcgt actcgcccat taccatcagt
     3841 agcgaagata cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga
     3901 tgtttgccct ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg
     3961 atttatgggg taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa
     4021 acgctgaggc aaaggcgctg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc
     4081 tgtttcgtcg ctgtggtgtg gtttgcttta gcagcaatta tgagctttac gcagacatga
     4141 gcaatcgggt gatgtcgacg ctggaagaac tatcgccccg cgtcgagatt tacagtattg
     4201 atgaagcatt ctgcgatctg acaggtgtgc gtaattgtcg cgatctgact gattttggca
     4261 gagaaattcg cgcaacggtg ctacaacgta cccatcttac tgttggtgtg gggatcgccc
     4321 agaccaaaac gttggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg
     4381 gtggggtggt ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg
     4441 tggatgacgt ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca
     4501 aaaccgttct cgatttggcg gatacagata tccggtttat ccgtaaacat tttaatgtca
     4561 tgctcgaaag aacggtgcgt gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg
     4621 caccgacgaa gcaggaaatt atctgttccc gctcgtttgg tgaacgcatc acggattata
     4681 cgtcgatgcg gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg
     4741 agcatcaata ttgtcggttt atctccacat ttattaagac gtcaccattt gcgctcaatg
     4801 aaccttatta cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg
     4861 atatcattaa cgctgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc
     4921 aaaaagcggg cgtgatgctg ggggatttct tcagtcaggg agtcgcgcag ctcaatttat
     4981 tcgatgacaa cgcaccgcgc cccgggagtg agcaattgat ggcggtaatg gatactctga
     5041 atgctaaaga gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc
     5101 agatgaagcg agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg
     5161 tcaaataaat atagcggcag gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga
     5221 ccgaacagat cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca
     5281 atcaggtaag cgataaaaat accgagcagc cactgcggca tttccatacc taaaaaatcc
     5341 cactgacgct cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc
     5401 ggcagccatt ccgggaaacg aaccataaaa tcacaggtgg caaatggcga aggatagagc
     5461 tgaagcatgg tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata
     5521 accatcgcta cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc
     5581 agaacgccga ataacgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc
     5641 acatgctgga accacagcgc cgtcagttcc agtgccagag cagtaaacgc catcaacagc
     5701 cacgcgcccc ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa
     5761 tatgcataga gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa
     5821 ataggacgca attggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt
     5881 aagttgttat caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc
     5941 aagcgtaaac tcgacgcaga gcaagccgac gagtgtcagt acgagggtgt aaggcagggc
     6001 catccacacc atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag
     6061 gaacaggaac gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt
     6121 aatggcgacc gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc
     6181 tttcgcttcg ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag
     6241 accgttgaaa atatagaaca gcgacagctg agcatgttca gatgcctgca acacaaactg
     6301 aataatcggc gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt
     6361 caacagtgcg gtgaatggca gagattcggt gaaggcttta ccgatagcat gttcatcggt
     6421 gacaccggtc aatgatgttg ccagaataat gactgacaaa ccaatcaagc caacttccgc
     6481 cagatgcaac gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg
     6541 gattttatcc tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcaacacttc
     6601 gcggactttc tccggcagcg tttcaccgta gccaaaccaa cgcagcttct ctaccagcag
     6661 gcaggttaac aggccacaaa tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa
     6721 atcgccaaaa tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat
     6781 ggtcattacg ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa
     6841 gccacggaac tgttccagaa ccacgttgta atgcttgtcg atatgactat cgtcttgcag
     6901 gtcggtgtct tcggtacggg aagaggctac gcgatgataa ataccataaa aaccgactgc
     6961 aacgctgatc accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc
     7021 cgccacgcaa aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt
     7081 aaatatgaac agcaacagct gtttcataaa atagataccc gccaccataa acatcaataa
     7141 cagcaagact tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc
     7201 gatgaatacc gcttcgatag ccaacagacc accggggagt agcgggtagc atttcagggc
     7261 catcgccaga gtgaaaataa attccgctac cagcaaccag cccgcgacga aagggctgat
     7321 gaggaaaatt aacgggttta cgattaagaa aatgatgagg gcgagtttgt accagtcggg
     7381 ggactggccc aaaaaattgc gccatagcgc gcggccccag gagatctcca tgatggtttc
     7441 ccttacctta caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg
     7501 caggagtgag gcaagtcttg atagtcaagg ggaaagagat gcggaaaatg aagccttgat
     7561 ccctttttct tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa
     7621 ctttgttttg ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg
     7681 tttcgcggaa gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat
     7741 tttgcccgca gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga
     7801 agtgttacag cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa
     7861 ggtgaataat ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga
     7921 tcacgaaagt gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac
     7981 tatttttatt cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac
     8041 cgctaatgaa gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg
     8101 cggcctggcg tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg
     8161 gctgtatacg cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct
     8221 gggcttctac cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga
     8281 aacagtgcgt cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct
     8341 gccgggtgat ttagccattc aggggcgata atcccttccg tttaaagagc aaccccctca
     8401 aacgaggggt tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca
     8461 actcgatgct gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat
     8521 gcacatgctt caggacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat
     8581 aacgcagcgt cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct
     8641 ccagattact taagttatat tgcgacgata accggttacg gatctcccga taaccttctt
     8701 cattatgaat agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga
     8761 agcggaaatc acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga
     8821 aatcacgcat cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc
     8881 agtatttgtc ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga
     8941 acccgagggc atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga
     9001 ccacattggt gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat
     9061 catacagatg gttaaggatg gtatagtgcc agaaggtcgc acagccttcg ttcatcacct
     9121 gagtctgttt ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac
     9181 gctgccatga ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg
     9241 gttcggacgg atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg
     9301 tacgccagag catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt
     9361 tctcttcttg cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca
     9421 gcgcgtggca cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact
     9481 cggtaatata tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac
     9541 tgcggaataa gcaattgttt ttaaagaaag agttatgtcc atagcaggcg tgtgccatca
     9601 ccagcgcctg catagtaatg gtgttctctt ccatcaagta agcgatacac gggttagagt
     9661 taatgacgat ttcataggcc agcccttgct gaccgtgctt atacagccgt tcagtctcga
     9721 taaacttttt accgaatgac cagtgcggat agttaattgg catgccgacg ctggaatagg
     9781 catccatcat ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt
     9841 agagtttcgc cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt
     9901 cgggtccatc gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacgcg
     9961 cacctcattg ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg
    10021 ttaaaaaccg cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac
    10081 taatccgctt ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta
    10141 aagacgccat attgccgcag agtcagggag atgtgagtca gctcaccata aaaaagccgt
    10201 atgttgaata atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact
    10261 gtatctaccg ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc
    10321 gttgccagcg cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag
    10381 ccgggggccg ccctggaaac cagtgccgcg aatgccgggc aaatctcccc cggatatgct
    10441 gcaccgtggg cagcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat
    10501 gcgccgctgg cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg
    10561 ttacgtaact gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg
    10621 gaatacagcc gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg
    10681 cgtcagggtg ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc
    10741 gatatcgccg tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg
    10801 gcggaagtgg atcccgcgct ggcagaagta gcgcacaaac tgacgggcgg cctgcagtta
    10861 cccaatgatg aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag
    10921 caggcggggg ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag
    10981 caaatctacg gcgtgaagtt tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg
    11041 tttggttctt actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg
    11101 aaaggctact cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt
    11161 cttgatgaaa cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga
    11221 atggcggaaa ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa
    11281 atggtggttc gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg
    11341 ggtttgcgcc cgatgacgcc agacggtacg ccggttgtcg ggagtacacg ctttaaaaat
    11401 ctgtggctga ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag
    11461 ttgttaagcg atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta
    11521 gcgcgctaca gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc
    11581 taaggaaacg agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag
    11641 aatctgtcca ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg
    11701 aacgcttacg ggcatggtat tgagcgtatc tggagcgcgc tcggggccac cgatggcttt
    11761 gcattactta acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc
    11821 ctgatgctgg aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg
    11881 accacctgcg tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca
    11941 ccgttggata tttatcttaa agtgaacagt gggatgaatc ggttgggctt ccagtccgat
    12001 cgcgtgctta ccgtctggca gcagttgcgg gcaatggcga atgttggcga aatgaccctg
    12061 atgtcgcatt ttgctgaagc ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt
    12121 gagcaggcgg cggaggggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg
    12181 tggcacccgg aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg
    12241 ccgtccggtc agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc
    12301 agtgagatta ttggtgtcca gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc
    12361 tatactgcgc gcgatgaaca gcgaatcggc attgtcgccg caggatacgc cgacggttat
    12421 ccgcgccatg cgcctaccgg tgctcctgtt ttagtggacg gcgtgcgcac catgacggtg
    12481 gggaccgtct cgatggatat gctggcggtt gatttaacgc cttgcccgca ggcggggatt
    12541 ggtacgccgg ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc
    12601 ggaacggtgg gctatgagtt gatgtgcgca ctggcgttac gcgtcccggt tgtgacagtg
    12661 taacttgttg taagccggat cggaggcagc gtcttctggt tgcgaaaaaa tcatccatcc
    12721 ggctggtcag caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt
    12781 aaaagaatgg acactatttc cccggaacct gaactcgccg cacaggcgtt ctacataaaa
    12841 cgcttacgct tcattgttga ctcggagtcg acttcagata aatcacgctt cacccttgat
    12901 gcagattcgg gttcatgttc agattgttga ttaacaggct gcgagtcctg ttgtctttcc
    12961 agactcaaca ggcccaactg ttgatccagg agctccaggt cctcctgtac cttcacgcgc
    13021 tccaggagtt ccgggtcatc caggaactcc agatccataa ggtcctccag ctcctcgagt
    13081 ccttccaggt caacctagcc ttccttgtgc aaaaagtcca aaaggtcctt caggccctgc
    13141 tgttttttca ggccctcgac ggctttgagc agcgtcggta aattgtcttt gtcccacggg
    13201 ttaccattac agtcaaacac cttcaggctc tggggctgcg gcacctgtac cgttttaaac
    13261 ttgttttctc cacagtagta aattattagt ttgggcggca gccgtggtga agagatcaaa
    13321 tcgttactag aacagtcaaa tactttcagg cgagtcaagt tctccagcat ctcatcaggt
    13381 attgagttaa tattgttgcc ggcacaggat agtttataca ggtactgggg catcgacggc
    13441 ggtaaaaatt gcaagaagtt ttcagaacag ttaatcactc tcaggttagt cgacagctca
    13501 ggcagccaga taatactgtt ttcatagcac ttcagtacca tcagttttga gggctgcacc
    13561 ttcggtaaca agatcaagac gttatctgta cagtcaagca ccagcagcca ctttggcatc
    13621 accttcggta acgcggtcaa cacgttttta ctgcagtaaa attcaatcag atgtggaggc
    13681 aaattctcag ggagtgaagt caatcccagg ttgctaagat tgagcatact ccgtcttgta
    13741 atgagacatt ccttcattct gcgcaatgct tctgcccggt tttcaatccg gcttgctggc
    13801 aggttttctt ccaggatatt ttccttgatt tcttccagac tttctttcag gtcttcttgg
    13861 ggaatttctg actgtttgtt ttgctggttt tcttccagga tttcgtacag gtttttttgc
    13921 aggttttctt gcaaaccatc ctgctgtata gcccattcgc accatttggc gaccatcatg
    13981 aactcgttat gttgctgatt cagacgagtc cccaacgtga tgatggtatt gttgtccacc
    14041 tgtatgttca ctttgcccgc gatactgagg tcaatgatct gccagggggc atcaccccag
    14101 ctgaattttt gcgggctgag atacttcatg ttctgcaaaa tttgattctt gagaagagcc
    14161 gccctggcgg gcgtcgtacg cctgggttaa cggaagatgg gggaatggcg ggggcgaatg
    14221 ggcagatgtc tttaaagcta gccataatgc tacgtttcct tgaaataaat atcctctggc
    14281 agcgccggag acgtgaatgt ggtcaccgca aaggagccac aggacgtcat ttgcgctgat
    14341 atcactactc atcgttgact tttttcctgg tcagaaatgc cgtgtcgggt ctggtacatg
    14401 ctgtattacg gcctaggtag ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg
    14461 aaataatcgt tattaagcaa aggaataact ataagaaatc gctcatgcat ccggtgctac
    14521 aggcggatga aacgcctatt gtgtgggcta tgtcagtgcg gcaggttgcg tgggggaata
    14581 tgttcaggcg atgtcaggca ggggcccctg atggttgacc ggtaatgcgg ggctggtttt
    14641 tattacctgt tacagtcgga aaaagccctt acgctgactt tcttgagccg gtgttttgag
    14701 ttcattagcc agagcctgca atggtttgtc atccataatt taaccttctt ctgatgttgg
    14761 gatgagcata tcaaaatcag gaaatgacaa gtttattaca ggctcaggct gcgttcagta
    14821 aagcatcgca tccggcgata gatgctgcat taaatttaag attcagcttc ctcttcagcc
    14881 acccgaacgc caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc
    14941 ccggcaaact ctacctggtc accgacaacc ggtgccgcgc ctagcaactg ctggacaatt
    15001 tcacccagcg tttgctgttt atcacgatat tctcgcccgt cttccaggcc atatatcagc
    15061 gcaacatcag catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc
    15121 agcgcgaccg gtggcgactg gctgaacagt ttaccgagcg ccgggagatc gcgttcccga
    15181 ccaattacac acaatacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac
    15241 tggttatcac gaaacagggc tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt
    15301 gccgcaccta cacaccattt atcagcactc aattgataaa caaactgctc ccacggattt
    15361 tccggatgaa tatccaggcc aacgcgtgac accggacgtc cgacgggcgg aaccaccact
    15421 ttggcttttt tcgccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc
    15481 acaaagaagg cgacattaaa gaacagtcgc gcattctcca gccccgccat catcgggaac
    15541 acggctagga tgattggcac cgcgccgcgt aatcccaccc agctgataaa cacgcgctca
    15601 cgcagattga aaccgcggaa agggagcaat ccagcaaata ccgaaagggg acgggcgaag
    15661 aatatcatcc atgccgacaa aatgagcgcc ggaatggcaa taggcagcag atcgcttggg
    15721 ttaaccaata gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg
    15781 aaattttgca ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac
    15841 agataaacag ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata
    15901 ccgccgctta atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt
    15961 tgcagcagta aataaccacc gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga
    16021 atatcgacca cgaacatcca actgacactg ctttcatgct gctggatcat tgcaatcagg
    16081 gtaatcgtca gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag
    16141 ccaacacgtt cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggtg
    16201 gagccgacga tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc
    16261 gccatcatgc cggttaaacc agaggtgata agtacgccca gcgtcgccag cgacagcgcc
    16321 ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata
    16381 atcgccagcg ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg
    16441 ccgacgccat cgactcccgc cagcatgccg atcgccagaa aaataaccag aataggaatg
    16501 ccaagacggg aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag
    16561 atgaaaaggc taattattgt tgtggcatcc aatggtcggt tactcctgat tacgctgtct
    16621 cttatataaa ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg
    16681 atttttttac attttaagaa caggatgacc gctaatcgtc aactgagcgc cttcccgggt
    16741 gttattcaga atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa
    16801 atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc
    16861 caggttgtaa cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat
    16921 cgccttctga cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg
    16981 gaaagggtgc acgttaatat cttccagcac cagaataccg ttctcaattt ttggcatcca
    17041 cggtgtacca atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt
    17101 ctgacatgtc gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag
    17161 ccagaaatgg tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg ctaccagcat
    17221 ggggccgcta aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa
    17281 atcgctatgt ccgcaaatga gtaacgggtc atgttgttgg cgagccacca gtgcctgcca
    17341 gtcaatatct gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt
    17401 gttgggggta gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc
    17461 cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta ttgacttgat gccccgcgtc
    17521 ggtaaggcgc tggataccac gcagcgcggc gtgctgttta atgctgtaac ccgatggggc
    17581 aattaagtga aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca
    17641 tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa
    17701 gtgaaattga gatggtttgc ctttttgatt gtgttattag cgggttgttc atcaaagcat
    17761 gactatacga acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg
    17821 ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt
    17881 atcgctatcg aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg
    17941 atgcagttaa aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt
    18001 gagccgacga ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac
    18061 ctgaatattc tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat
    18121 gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat
    18181 cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta
    18241 gcgcgaaatc atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg
    18301 gacgcgatgt aaatcagtcg cgcactttgt ccgctttttc ccgggcttct cgctcgagag
    18361 agaaaataat ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc
    18421 tcagacgggg agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctggtgg
    18481 agattaactg ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatttgag
    18541 cgaagcgcat accttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc
    18601 cgcctaacga caaatcatac aagcggaaac gtaacgtact gttatccgcc agtttggtct
    18661 ggcaaaaata aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa
    18721 accataaggt gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct
    18781 gtagttgttc aacagtaaac tcgactttcg caccctgagt ttcggcggta atggtaatgt
    18841 gctgtgcctt tagcacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt
    18901 tatccggggt tattgccagt attttgctga tcagctgccc gccattccaa ctgagacgca
    18961 aaggaattgc ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt
    19021 tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt
    19081 ggtttatcgg tcatagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg
    19141 ttcttaaatc agccgacaaa cgcccagcat cgatcccgcc cttgcctata cttagagcgt
    19201 tgacgtaagc atttcttgcg accgattcat cgaaacgagg gcctgaacat gggaattatt
    19261 gcctggatta tttttggcct gatagccggc attatcggca agctaatcat gccggggcgt
    19321 gatggtggtg gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc
    19381 tggctggcga ccatgtttgg cattgacggc tccatcagcg gttttaattt gcacagcttc
    19441 ctggtggcgg tggtgggcgc tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa
    19501 taagattttc ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg
    19561 tatactcgac acctgcccag aaattgcgac cttcttcaat aaacccttca ctgtaggcat
    19621 attgggtatc aaacaggtta ttgaccgacg cattgacgct gaagccatga cctaaggtgt
    19681 aatcggctcg aatgtgggtc accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc
    19741 tgtagctgga ggaacgcgcc tcttccgaca gcgttacgct taacggctcc catggtttga
    19801 gtgtcatcca tgcggtcatt gtctgcgttg gaagatcggt tatcttgccg atgtctttgc
    19861 gtttggcgtc agcgtggatc agagcgtagc tcaatcctac atccagtata ttgctgattt
    19921 tccccttaat accggcatcc agaccgctgt aatccaccgt gccgctgttc tgattttgaa
    19981 tggtatcggc attgatattg tgcgagagga tggcatcact cacccggtta tagtaaacgc
    20041 tgacctcaaa tccccagtcg cgcgtgaagg caccattcca ggttaaatcc accccgcgag
    20101 cgcgttccgg cttgagctgc gggttaacaa tcgctatctg gttatacgca ggtttggatg
    20161 tggtatagcg ttctttcagc gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag
    20221 tgtcttcatt ggcaaagtgg tatttcccca tcacctgcca gttaaaagct gactgattgt
    20281 tgtcgtcata atgggtgatg ctgccatctt tctcgtgttt tttcgcttct acgctatcgc
    20341 gccagtcata gctgattccc gccacgacat cgacattatc agcagcagcc cattgatatt
    20401 cactggcgag cgaccaggta cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt
    20461 tttcccggtg tacgtcatct ttccagttaa cggcaaacga cagcagatcg ttttcacgca
    20521 catcggctgc cagttgtaat ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc
    20581 cttttttatt tttcaaatca gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt
    20641 cgcgatacag ccgacttttc agggtaaaac ggtcgtttag ttgggtcgct ccctgataat
    20701 aaaaactttc tttgtcatac tctggccact gccagtagcg tgatttttga ccacaatttc
    20761 cgctgtatgg cgggttatct ttttcaccat cctgcttaat gtaagtcagc gtgtattcat
    20821 cgttttcgcg cggtgtaaaa ccgagcttca caatgccgcg tttatcatca gccgatgaat
    20881 taatcatctt gccgtgtttg cctgcaatat cattatttac accatgcggc aggccgagaa
    20941 aatcctgctt tagctggcta ccgctgactt gcaaataccc aagatcgctg ctggcggcaa
    21001 atgaagcatg catatcgtag gcattgtcct gactacggct ccatccctgg cgatatccca
    21061 gacttgcttc ttcagggtgg tgatattaat ggctccgccc atctgattag gcccatgaag
    21121 cagcgacgaa taccctttgg aaacttcgac tgcccccaga ttgttggtca gaatccgcgc
    21181 cagatcgagg ttgccgtcat aggggacata aatgggcaca ccgtcgaaat agaccggcac
    21241 ctgccgacta tcaaagccac gaacttttac ctgttcttcg ttgcggctgc ctgacttttg
    21301 cagcaccacg ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccaaggcctt
    21361 catggtgggt tgatcgagaa cggtggtcgt cgtcgatgat accggactgg accatacggt
    21421 cagcgtgtcg ctctcctgag ccgctacggc ctgctgggta aaggcaagtg ataatgctgt
    21481 gcagagataa cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag
    21541 aggctgtggg ttgcagactg actttaatgt cagctggtgc cgcgtaataa ggcgcggagg
    21601 taataaaaag gcgaatgccg cagtcgaggt aatttttgag tgtcgtcaga ttaattccgc
    21661 cggtcagagc cagcgtgcag tgaggagcca gcgacggcgc tatttgcgca atttcagttg
    21721 cctgttgcgg actaaatttg tcgagctgaa gtacgtctgg ttgtgcgcgt aacgcggcga
    21781 tagcctcttt cggcgtgtcg gcttcaacaa caattttctt ctcgggtgcg tggcgacgta
    21841 actgagcgat tgcgcctgac cagccctgat tgtcatgaag aaaatggcgg tggttggcaa
    21901 acaataatat ggtttccgca catccggcac gatgaattag tcctccggca gccagaattg
    21961 cctgcgaagc cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattgccgt
    22021 caggataacg ttcacgaagc aatgccagca tttgagcgag ataatcagaa acaccgcaac
    22081 tccactccag cacattctgg actgccttcc atccctgatg aagtgctgcg gcattaccct
    22141 gcgcacggat tagccgttga cccgcgttcg cttgtgaacc gtcgctgacc gcgtcatcaa
    22201 tagttaatcc cagcgtagtt aacatcttac acgcgacaga aacgccgctg acgcagccgc
    22261 cctgacggtg aaaaaacgct atatagccag gctggtgtcc aatatttaat gctcgcgtgg
    22321 tcaggtcacc tccctggata tcttccagca gtaacgcatc gatttgtgcc tgagaaagga
    22381 aaatcatcac agtgcctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct
    22441 ggcaggggcg agggcacgag catcttgttg ttgataccag tgatataacc gctcacgctc
    22501 atcgtcgtta agcgcgccaa gactccagct gacgttctgc tcaaaacgtt cccaggtgct
    22561 gttatcctgt tggcaatttt gtccacggat aaaatcaacg tgggcataaa ttcccatctg
    22621 atacaggacg ttgagcgcaa agatatagtt gggtaattcg atcacttccc gaccagccgc
    22681 ccgctgaatg gctggagaga cgaacgaggt gctgaccaga tgagttgtat agacgcgaag
    22741 ccgtgcctga ttattcagtt tgctcattgc atggcgcata tcagccacca gagtggagcg
    22801 ggaagccacg gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg
    22861 gatccagtgg acattgtcag ccttcagtgc ggcggcgcgg cgggcggcaa cgttcagcat
    22921 cccctgactg taatcaacgc cataaaccgt agtgagtttg tcagccagcg ccagcgatac
    22981 ggtgcctggt ccgcagccca tatcgaataa ggtctgagcg ccttgcaggt caattttagc
    23041 gatcagttgt tgcaggtagc tgtccgttgg cgaggcgcaa ttttccgcca ttttctccgc
    23101 ccgttgatcc cagtgatcgg gcgttttttc tgtgcggtga gccagtttga gttgctgcag
    23161 ataaaggtct gcaaaatcaa tatcatcaat gagcatgatt gttccttagc ggctaattgc
    23221 agagaggtga tgatgaattt ggtcggcaga aacccgatac agggccgcca gtttgttcgt
    23281 tgttaactgt tcggtcggta agccttgtgt tactcgtcca tctggctcta cttggataat
    23341 gctgtcggca atggcgttgg catggagcgt atggtgagtc gacattaaca ttgtcatgcc
    23401 gttatttttg agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct
    23461 ggaggcgggc tcatccagta acaacaagcg cggttgttgg acgagtgcac gggcgattaa
    23521 agccagctga cgttcaccgc cgctaagcgt attccagcgt cgtgtcgcca gatgcaaaat
    23581 gcccagcttt tccagttgct ccgtggcttt caggcgttct tgcttaccgg gaacggaaaa
    23641 agcgcctatg gtgggggcca ggcccatcaa caccatatcc agcacactga aagcaaaaat
    23701 accgtcgtgc gcctgcggca cccaggcaat agcctgtgcg cgctggcgat ggctgagtgt
    23761 ctgcacagag aggctatcca gtaggatctc gccttttaat aacggtataa cgccaagaat
    23821 ggagcgcatg agtgtggttt tgccgcaacc attcgcgcca agcagacaac aaattgttcc
    23881 ttgcgacagg gtgaaggaca cgtcgcgtag cactggatgg cgggagtaac ccagagacgc
    23941 atgacgaacg gcaagcaaag tcactggcga cctccgcgta acaacaaaag caggaaaaac
    24001 ggtgcgccaa caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgc
    24061 gccagcgtat cggtaagcag gagcagaata gcgccagtac acattgccat aggcaacagc
    24121 tgctgatggt tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag accgatccaa
    24181 ccaattatgc ccgctattgc taccgtgctg gcggtgatta gtgtggcgca gacaatcaat
    24241 ccaaagcgca gacgcgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg
    24301 agtaaattca tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt
    24361 gcagcatagc agagatcgcg taaggtcacg gtggacagtc cccccagcaa ccagaaggta
    24421 attgacggta actgggtgta aggatcggcc agcgttttaa ttagcgatat ccccgctccg
    24481 cacaacgttc ctaacgcaat gcctaccagc accagggtta agatcggatc gtggcgggtc
    24541 acccgacgcg taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac
    24601 agctggatgt aaacaatggg taatccccac aggattgccg cgcaggcacc aagtccggca
    24661 ccggcggaga cgcctaaaat atccggtgaa actaagggat tacgcaacat tccctgatag
    24721 gtggttccgg ctcctgccag cgctgcgccc agtaacagcg cagcaagaat acgcggcacg
    24781 cggatttgcc agaagacaat ttgttcctgc acagactcat tttcttgacg aagaattagc
    24841 gccagcagtt gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg
    24901 atagctgcca gcagcaaaat acatccgttt acgattctca ttagctcgcc accaattttt
    24961 gatagtcggc atcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac
    25021 ttttaaactg gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa
    25081 gacggttgat tcccggcggg gcatcgagcc agccaaaggg caagccactt aaaaataaga
    25141 tgcgttgttc cgcaacggct ttcacgccct gccagagagg atcacgacga ataaaatctg
    25201 cagtaacggc ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg
    25261 aaacctgtgt caggccgtgg cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt
    25321 ccgccgcctc ggtatgcaac gaaccctgta agcccgtttc cagccctcgg ggaccgcgcg
    25381 cagcataaaa gcggaggtta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga
    25441 agtggctggc gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa
    25501 gcgttgttaa ctgttctgct gattgcgcca gttttccgtt aagcaataac caggggattt
    25561 gtgtctgttc gctaacctgc cgtgcctggg agatcaaggt ttcatccgta ttgccgcaat
    25621 caacgaccaa atcgggatgt aacgccataa gcccttccag cgatagtgtg ctggcgcgcc
    25681 cggcgagtcg tcccagcctg ggtaactggc gaatgtgctc tggcaatgga attaatgcct
    25741 gacgggcaaa atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaaga
    25801 gcaaatcggc gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagag
    25861 attgcgaagc aaaacgatta actgtttgtg aaaaggacgg tagagactgc aggagcaggg
    25921 tagatgcaag tgcctgcgca aacgtccgtc tggttatggg catggtgaac atcctgaaaa
    25981 gtaaaatcga tatatacttt catatatatc gataaaaatc atgttcaata tcaaagttta
    26041 gctattgaaa acatgaagat gtgacgactt ccgcaagcct gttgaccctc tctatcatta
    26101 aaaatgtatt tatcaggtgg ctaatattat tt
//
LOCUS       DAJTRM010000055        26044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRM010000055 DAJTRM010000000
VERSION     DAJTRM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.055"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..392)
                     /locus_tag="OO322_002383"
     CDS             complement(<1..392)
                     /locus_tag="OO322_002383"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5907235.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVSKNHVMIVIEDLKVSNMSKS"
     gene            complement(525..830)
                     /locus_tag="OO322_002384"
                     /pseudo
     CDS             complement(525..830)
                     /locus_tag="OO322_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            898..1513
                     /gene="tnpA"
                     /locus_tag="OO322_002385"
                     /pseudo
     CDS             898..1513
                     /gene="tnpA"
                     /locus_tag="OO322_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1841..2923
                     /locus_tag="OO322_002386"
     CDS             1841..2923
                     /locus_tag="OO322_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5907236.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3412..4210)
                     /locus_tag="OO322_002387"
                     /pseudo
     CDS             complement(3412..4210)
                     /locus_tag="OO322_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4220..4771)
                     /gene="ybcL"
                     /locus_tag="OO322_002388"
     CDS             complement(4220..4771)
                     /gene="ybcL"
                     /locus_tag="OO322_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5907237.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4940..5272)
                     /gene="emrE"
                     /locus_tag="OO322_002389"
     CDS             complement(4940..5272)
                     /gene="emrE"
                     /locus_tag="OO322_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5907238.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5617..5931)
                     /gene="fliE"
                     /locus_tag="OO322_002390"
     CDS             complement(5617..5931)
                     /gene="fliE"
                     /locus_tag="OO322_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5907239.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6145..7804
                     /gene="fliF"
                     /locus_tag="OO322_002391"
                     /pseudo
     CDS             6145..7804
                     /gene="fliF"
                     /locus_tag="OO322_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7797..8792
                     /gene="fliG"
                     /locus_tag="OO322_002392"
     CDS             7797..8792
                     /gene="fliG"
                     /locus_tag="OO322_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5907240.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8785..9471
                     /gene="fliH"
                     /locus_tag="OO322_002393"
     CDS             8785..9471
                     /gene="fliH"
                     /locus_tag="OO322_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5907241.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9471..10844
                     /gene="fliI"
                     /locus_tag="OO322_002394"
     CDS             9471..10844
                     /gene="fliI"
                     /locus_tag="OO322_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5907242.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10866..11207
                     /gene="fliJ"
                     /locus_tag="OO322_002395"
                     /pseudo
     CDS             10866..11207
                     /gene="fliJ"
                     /locus_tag="OO322_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11204..12331
                     /gene="fliK"
                     /locus_tag="OO322_002396"
     CDS             11204..12331
                     /gene="fliK"
                     /locus_tag="OO322_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5907243.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12436..12900
                     /gene="fliL"
                     /locus_tag="OO322_002397"
     CDS             12436..12900
                     /gene="fliL"
                     /locus_tag="OO322_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5907244.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12905..13909
                     /gene="fliM"
                     /locus_tag="OO322_002398"
     CDS             12905..13909
                     /gene="fliM"
                     /locus_tag="OO322_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5907245.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13906..14319
                     /gene="fliN"
                     /locus_tag="OO322_002399"
     CDS             13906..14319
                     /gene="fliN"
                     /locus_tag="OO322_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5907246.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14322..14687
                     /gene="fliO"
                     /locus_tag="OO322_002400"
     CDS             14322..14687
                     /gene="fliO"
                     /locus_tag="OO322_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5907247.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14687..15452
                     /gene="fliP"
                     /locus_tag="OO322_002401"
                     /pseudo
     CDS             14687..15452
                     /gene="fliP"
                     /locus_tag="OO322_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15462..15731
                     /gene="fliQ"
                     /locus_tag="OO322_002402"
     CDS             15462..15731
                     /gene="fliQ"
                     /locus_tag="OO322_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5907248.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15740..16525
                     /gene="fliR"
                     /locus_tag="OO322_002403"
     CDS             15740..16525
                     /gene="fliR"
                     /locus_tag="OO322_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5907249.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16810..17434
                     /gene="rcsA"
                     /locus_tag="OO322_002404"
                     /pseudo
     CDS             16810..17434
                     /gene="rcsA"
                     /locus_tag="OO322_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(17478..17666)
                     /gene="dsrB"
                     /locus_tag="OO322_002405"
     CDS             complement(17478..17666)
                     /gene="dsrB"
                     /locus_tag="OO322_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5907250.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17829..18056
                     /gene="yodD"
                     /locus_tag="OO322_002406"
     CDS             17829..18056
                     /gene="yodD"
                     /locus_tag="OO322_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5907251.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18354..19169
                     /locus_tag="OO322_002407"
                     /pseudo
     CDS             18354..19169
                     /locus_tag="OO322_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(19166..20860)
                     /gene="yedQ"
                     /locus_tag="OO322_002408"
                     /pseudo
     CDS             complement(19166..20860)
                     /gene="yedQ"
                     /locus_tag="OO322_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(21031..21213)
                     /locus_tag="OO322_002409"
     CDS             complement(21031..21213)
                     /locus_tag="OO322_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5907252.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21292..22209)
                     /locus_tag="OO322_002410"
     CDS             complement(21292..22209)
                     /locus_tag="OO322_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5907253.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22382..23302
                     /gene="yedA"
                     /locus_tag="OO322_002411"
     CDS             22382..23302
                     /gene="yedA"
                     /locus_tag="OO322_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5907254.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23291..23761)
                     /gene="vsr"
                     /locus_tag="OO322_002412"
     CDS             complement(23291..23761)
                     /gene="vsr"
                     /locus_tag="OO322_002412"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5907255.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23742..25160)
                     /gene="dcm"
                     /locus_tag="OO322_002413"
     CDS             complement(23742..25160)
                     /gene="dcm"
                     /locus_tag="OO322_002413"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5907256.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25227..25767)
                     /locus_tag="OO322_002414"
                     /pseudo
     CDS             complement(25227..25767)
                     /locus_tag="OO322_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25807..>26044)
                     /locus_tag="OO322_002415"
     CDS             complement(25807..>26044)
                     /locus_tag="OO322_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907257.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         6553 a         6671 c         6415 g         6405 t
ORIGIN      
        1 gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattacgtg gtttttgctg
       61 acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt
      121 cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt gcggcttaac
      181 tgtcgctgaa gtctcgccag cgtcttctgg tttttctgaa aactgtttac aggctcaaag
      241 actgtgccat ctgacagcga ggcaagttta gccacgccag catccagccc gaccattgat
      301 gctgaagggt gaaccggagt tgatacttca ctttctgcct gaatactgat gtaccactta
      361 ccgcaggact ggctgacagt gacatttttc acaacacccg tgacctgacg gctgttccgg
      421 tagcgcatcc agccaagttt tggcagaaaa atacggctgt tttcctgagc gagcttaacg
      481 ccctgcggat agcggaatgc atcattctgt caccgctttt tgaatcaggg gaacgccgag
      541 cgcttctgga aaaagttttt gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc
      601 tgtgacggag catctttaat ccattgcgtt tcagtggcat ttttccactc aaccaatcag
      661 gaagccattt tcgcgtaagg gatgtctttg ttaccggcct tatgattctc attctgacgt
      721 gccagagtac gactgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc
      781 tgttgaccac cgggtcttaa ccggaatttg aatacctgta gtcgtttcac agggcaaatt
      841 atactttggt ctgtgaaaaa aactgatatc cgacgcggaa ggcactgtgt tttcctgatg
      901 catgctcacc gggtctttgt taccaagtac cgacgccaga tttttgacca tgacgcgaca
      961 gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa
     1021 atggatggcg aaccagatca cgtccatctg ttaattaact atccccccaa actggcgata
     1081 tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc gacgggatcg
     1141 accagatatt gctgccaggt attaccgcaa agatgttttg tggcgtcccg gctattttgc
     1201 cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac aacagcaaac
     1261 acctggtcag atgaaaaaac gagccttata tccccgccct gaaggacggg cttttacggt
     1321 gcactggata agcctggaga ttatctacac ctgcatggac atcattgtac tatgccccgt
     1381 acatgctcca tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct
     1441 cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg gcgctatatt
     1501 gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat catgcaaata
     1561 atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag tcaatcataa
     1621 ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa atagtcatgc
     1681 attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa tttaaatgag
     1741 aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg tagtgcagtg
     1801 acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat taacagtggc
     1861 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag cagagattta
     1921 caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc actacttctc
     1981 ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac
     2041 tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
     2101 tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg gcctgaaatt
     2161 cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg atatcggtgc
     2221 atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
     2281 gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
     2341 tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg taactgaagc
     2401 taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg gtgtaggtgc
     2461 aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca acagcctgaa
     2521 tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg cgaacaacat
     2581 ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta ataaccatat
     2641 tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact tcggtctgcg
     2701 tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga
     2761 cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata tgtccacttt
     2821 tgttgattac aaaatcaacc tgattgataa gagcgatttc acgaaagcat ctggcgttgc
     2881 taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt actcaaagat
     2941 atgttgtggg aggctttgcc tccccaacat ataagtggcg ctctcaagcc actttcctta
     3001 gaagcactat cttgcttctg actatataaa ttacccttta ttttctggga cgtttcaacg
     3061 ccccattttt aataatactc agtgaacaat taacgtatca attagattta ataacaacga
     3121 tatccgtacc taacctgata tctaatgtca taagcatccc tttaattatc ccctcaacct
     3181 cttgtaaatt tcccactctt gagctccaca tagcccaaac gccggatgca aaaaaaatca
     3241 tacgtgcatc tctgaaattg tgatggagga agtgagtatc aattatatgt gcttataaac
     3301 gttcccgatt ggcacagaag tcaccggatt gttcaggctc caataggttg agtatgtcgt
     3361 atttattttc caaaataaaa atcgatgctg ttgctcctta ataaaatact tttactctct
     3421 ggacgctttt cttagctctc ttggtgttat attaaaacga ttatggaacc tttcagtaaa
     3481 acgagaagga cacttgtaac cattttccct ggcaatctcg ctgatgggtt ttactgtcgt
     3541 ttgtatagca ggacaacgca ttatttaacc tcacgtcgtc caatatactt tgaaaactta
     3601 ttccctcgct tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta
     3661 ccttgtttgc tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta
     3721 ttaatgattg tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa
     3781 gtagaacgcc cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg
     3841 tttttagttg ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg
     3901 tgttacgaat ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg
     3961 gataataaag catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt
     4021 catatccaca gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc
     4081 aattgataac tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc
     4141 tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctc
     4201 ttgccagcat actcttaccc tattttatct cataaactgg tgttatctca gcggttgcaa
     4261 ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa
     4321 tcttatcagt ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg
     4381 gaggacatgc tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag
     4441 tcggaagttt tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg
     4501 gaatattagc aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca
     4561 cagttacggc aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg
     4621 tattaccacc ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg
     4681 tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg
     4741 ccaatacact tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta
     4801 ttttatttga tgtgccttga tctcattatg cataaaggcg caatgatcgc gcaaatgcaa
     4861 tcataattat aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta
     4921 tctcaaggag aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca
     4981 caccggcaca aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa
     5041 atccccagga aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa
     5101 tccctgtagg tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa
     5161 taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg
     5221 taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt
     5281 ctttcctgtt catactggag agaattgtac tacagtttga acacaactca ccgttttcat
     5341 cctggtgtat gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa
     5401 tgatcagaag gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga
     5461 caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc
     5521 cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga
     5581 tataattaaa caagcatagt caacaggtta cacaacctac acctgcatgg acatcacttc
     5641 ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc
     5701 tttttgcata tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt
     5761 ttctgcctgc gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag
     5821 ctgcccggca aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc
     5881 cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc
     5941 ccggataatt tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag
     6001 caacaaaaaa actggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt
     6061 caatttttcg agtgtgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt
     6121 ccataatcag ccacgaagtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag
     6181 tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca
     6241 gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc
     6301 agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt
     6361 ccttaccgct tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa
     6421 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat
     6481 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
     6541 ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg
     6601 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
     6661 gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg
     6721 gtttccagcg cggttgctgg tctgccgcct ggaaacgtca cgctggtgga tcagggcggg
     6781 catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat
     6841 gccagcgatg tcgaaggccg tattcagcag cgtattgaag cgatcctgtc acctattgtt
     6901 ggtaacggta atattcacgc ctaggtcacg gcgcaactgg acttcgccag taaagaacaa
     6961 acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag
     7021 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
     7081 aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac
     7141 cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa
     7201 accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg
     7261 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
     7321 cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt
     7381 tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa
     7441 agcggcggag cactgccgtt ttggcaacag caagtgttta tcgatcagtt gctggctgcc
     7501 ggtcgctggt tgctggttgc tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt
     7561 acgcccgcag ctaacacgtc gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc
     7621 ccgcgaggaa gtggaagatg cggtggaagt ccgtctgagc aaagacgaac aactccaaca
     7681 acggcgcgct aaccaacgac tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc
     7741 tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga
     7801 gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg
     7861 cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg
     7921 cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag
     7981 aagctgaaca gtttgctgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg
     8041 tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga agatattctc gaaacccgcg
     8101 ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga
     8161 ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgagg cgcgcccagg
     8221 ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg
     8281 ccacctttgg cggcgtgcag ccagccgcgc tggccgaact gaccgaagta ctgaatggct
     8341 tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa
     8401 ttatcaacct gatgaaaacg cagcaggaag aagccgttat taccgccgtg cgtgaattcg
     8461 acggcgagct ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg
     8521 tcgatgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg
     8581 cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg
     8641 ccgccgatat tctgcgcgac gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg
     8701 aaaacgaaca gaaagcgatt ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg
     8761 tgattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg
     8821 gacgatctcg cgccaccacc ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt
     8881 attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccac
     8941 gagcagggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcacga acagggctat
     9001 caggaaggac tggcccaggg gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg
     9061 ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt
     9121 gatagtgtga tagcttcgcg cctgatgcag atggcgctgg aggcagcacg tcaggtgatc
     9181 ggtcagacac caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag
     9241 caagaaccgt tattcagcgg gaaaccacag ctgcgcgtac acccggatga tctgcaacgt
     9301 gtggatgata tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg gggcgatccc
     9361 accctccacc ctggcggatg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc
     9421 gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc
     9481 gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg
     9541 cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat
     9601 tacaattgcc gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg
     9661 aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg
     9721 aagtggaagg tgtcctgccc ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc
     9781 tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta
     9841 gcggtaaacc gctcgatggc ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta
     9901 ccccgccatt taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc
     9961 gcccaatcaa tgccctgctt accgtcggac gcgggcagcg tatggggctg tttgccgggt
    10021 cgggcgttgg taaaagtgtg ctgctgggga tgatggcacg ctacacccgc gccgatgtca
    10081 ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa agattttatt gagaatatcc
    10141 tcggtgccga aggacgtgca cgctcagtgg tgattgccgc tccggcggat gtttctccgc
    10201 tcctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg
    10261 gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa
    10321 ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca
    10381 aattaccggc actggtcgag cgtgccggta atggcattag cggcggcggc tcgattaccg
    10441 cgttttatac cgtgctcacc gaaggcgatg accagcagga ccccattgct gactccgcgc
    10501 gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc
    10561 cggctatcga tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac
    10621 attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc
    10681 tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc
    10741 tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag
    10801 cgtctctcca ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc
    10861 agatggcgga agaacagctc aaaatgttga tcgattatca gaatgaatat cgcaataacc
    10921 tcaatagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta
    10981 tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga
    11041 aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga
    11101 cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa
    11161 agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc
    11221 cttgattacc gccgacgttg acaccaccac attgcctggc ggcaaagcca gcgatgctgc
    11281 acaagatttt ctcacgttgt tgagcgaagc attagcaggc gagacaacaa ctgacaaagc
    11341 ggccccccag ttgctggtag caacagataa gcccacgaca aaaggcgagc cgctggtcag
    11401 cgatattctt gccgatgcgc aacaagcgga tttactgatc cctgtggatg aaacacttcc
    11461 tgtcatcaac gacgaacaat ccacatcaac accgttaacc accgctcaga cgatgacgtt
    11521 ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga
    11581 cgtcaccgca agtctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa
    11641 agtgactgat gcaccgtcaa ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact
    11701 gacttctgcg caactcacta cagcacagcc tgatgacgcc cccggcacac cagctcagcc
    11761 attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca cgccttcgcc
    11821 ggtgaccgct gacgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt
    11881 cgccgcgcct gttttgagtg caccgctggg ttctcacgaa tggcaacaat cattgagcca
    11941 gcatatttcg ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcacccaca
    12001 ggatttaggt gaagtgcaaa tttccctcaa agtggatgat aaccaggcgc agatccagat
    12061 gatttcaccg catcagcacg tacgcgccgc cctggaagca gcgctgccgg tactgcgtac
    12121 gcagctggcc gaaagtggca ttcagttagg gcaaagcaac attagtggcg aaagctttag
    12181 tggtcagcag caggccgctt cccagcaaca gcaaagccaa cgcacagcaa accatgaacc
    12241 tctggcgggg gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac
    12301 aggtaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct
    12361 tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca
    12421 ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg
    12481 atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg
    12541 cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc
    12601 ttctatgcgc tggatacctt tacggtcaat ttgggcgatg cggatcgcgt gctttatatc
    12661 ggcataaccc tgcgactgaa agatgaagca acccgctcgc ggctgagtga gtatttgccg
    12721 gaagtccgta gtcgcttgct gttactgttt tcacgtcagg atgctgccgt actggcgaca
    12781 gaagaaggca agaaaaacct gattgccgag attaaaacca cactttccac cccgcttgtt
    12841 gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa
    12901 cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag
    12961 cgaagtcaaa gacgaaccga cagccagtat tagcggcgaa agtgacattc gtccgtacga
    13021 tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga
    13081 gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgccgta gcccggacat
    13141 taccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc
    13201 gaccaacctg aacctaatcc acctgaaacc gctacgcggc acagggctgg tagtgttctc
    13261 gccgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac
    13321 caaagtggaa ggtcgcgagt ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa
    13381 gctggcgctg gaaggctata gcgacgcctg gaaagcgatt aatccgctgg aagttgagta
    13441 cgtgcgttcg gaaatgcagg tggaatttac caatatcacc acctcgccga acgacattgt
    13501 ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct
    13561 gccattcagc atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc
    13621 gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct
    13681 ggaactggtg gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa
    13741 acccggcgac gtcctgccga tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt
    13801 cccggttctg accagtcagt acggtactct caacggtcag tatgcgttac ggatagaaca
    13861 tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat
    13921 aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc gttgagcgag
    13981 caaaaatcaa ccagcgaaaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt
    14041 gatgtcagcg gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc
    14101 gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaagggtca
    14161 gtcgtggcgc tggacggtct ggcgggcgaa ccgctggata ttctgatcaa cggttattta
    14221 atcgcccagg gcgaagtggt ggttgttgcc gataaatatg gcgtgcggat caccgatatc
    14281 attaccccgt ccgagcggat gcgccgcctg agccgttagt gatgaataac catgctactg
    14341 tgcaatcttc cgcgccggtt tctgctgagc cactgctgca ggtgagcggc gcactcatcg
    14401 ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggctggga tttgccccta
    14461 aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac
    14521 gggtcgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa
    14581 tcaatctgct gcataaactt cccccttcag caccaacgga agagataccg cagaccgatt
    14641 ttcagtcggt catgaaaaat ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt
    14701 ttctgtcgca ccggtccttc tctggctgat tacgcccctc gccttcgcgc aactgccggg
    14761 tatcaccagc cagccgctac ctggcgggta tcaccagcca gccgctacct ggcggtggac
    14821 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    14881 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    14941 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    15001 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    15061 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    15121 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    15181 ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga
    15241 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    15301 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    15361 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    15421 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    15481 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    15541 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    15601 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    15661 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    15721 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    15781 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    15841 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    15901 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    15961 cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    16021 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt
    16081 cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata tgctggcatt
    16141 actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt
    16201 tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa
    16261 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgtt
    16321 gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt
    16381 tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc
    16441 gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat
    16501 tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc taaggattat
    16561 ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat atttgtttta
    16621 agctcactca catatcgcaa catttacttt aagacaattc caggcaaatt atacaacact
    16681 ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt
    16741 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
    16801 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
    16861 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
    16921 gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt
    16981 ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat
    17041 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
    17101 agaaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct
    17161 tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc
    17221 attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca
    17281 aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa
    17341 acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac tgacggataa
    17401 tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc
    17461 agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg atgccctgct
    17521 tcattaaaga accagatgcc gagcgggtag tcttccagcg caaccaggta cattgtgcct
    17581 tcactaaact cctcaactgc cagtaccact ccaggacgac gcggaccgcc atccgttttg
    17641 actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa
    17701 caatttcgtt tttctggcag cgccaagcgc gcgcgagtgc tgattttctc gacggtctat
    17761 acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa cggttgcgag
    17821 gaaagatgat gaaaaccgca aaagtgtaca gcgataccgc aaaacgtgag gtcagcgtcg
    17881 atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt catcgcagcc
    17941 agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg cgtgaattgg
    18001 cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat cgttgaatgc
    18061 acaataaaaa aatcccgacc ctgagggtgt cgggatgaaa cttgcttaag caagaagcac
    18121 ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatctg caattttttt
    18181 cgctgacaag aaaatattcg cgctatgaat gattaacgct acttatgaat agaaatgtga
    18241 cgcgcgtcac cccttggtgg ctctggtgtc aacgggatcg ctcccgaaaa aatcatcttt
    18301 gattatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgcg accatgtttt
    18361 caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg gacagtcata
    18421 gttatgactg gcaaccggca gccccctggc tcagccgttt acacgaagcc aatattcccg
    18481 tcattctctg tagcagtaaa actttagcgg aaatgctgta cttgcaaaaa atgttggggc
    18541 tacaaggctt accgctgatt gcagagaatg gcgcagtgat ccagcttgct gaacaatggc
    18601 aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa atctgccagg
    18661 ttttaaatac gctacgcgag aaagaacatt ttaaattcac gacttttgat gatgtcgacg
    18721 atgcaaccat tgccgaatgg acgggattaa gccgtagcta ggcggcgctg acgcagctgc
    18781 atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca caatttatcg
    18841 ctcgtctgaa cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg cacgtactgg
    18901 atgcctctgc cggaaaagat caggctgcca actggattat cgcgacctat caacaattgt
    18961 caggcagacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg cctttactgg
    19021 aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggta catctgcatg
    19081 atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc tggcgtgaag
    19141 ggctggacca ttttttctcc gcccgttaag cgttatcgct cgcgaatacc cgattgcgcc
    19201 cggcctgttt tgcgaggtaa agccgacggt cggccaatga ctggagttgt tcaaaatcat
    19261 aatcaccggt ttcctcgctg ctacttaccc ccagcgaggc actgatgcgt atcgttgtac
    19321 tcttggcgat cagcatctct ttttcattta acttcaggcg aatacgttct gcgacctccg
    19381 cagcctgcgt cagattcgcg cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc
    19441 cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga gaaagaacac
    19501 ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca agatcgacct
    19561 gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga cgggcttttt
    19621 cgaacagtgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc tgccactgca
    19681 acgagctttg cagaacatac atgttgctga ccatccggcg aatcacatac caggagagga
    19741 gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata ctgccgaaat
    19801 cgccgcgcac gccttcgctt agcgtatgga cgcgcgccag cacaccgtcg aaatggtcca
    19861 gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg tcatgttcca
    19921 ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt ggatggtcag
    19981 gattggaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca ccatcgaggt
    20041 ttttatcgat ggcgtttctt aaaaattgct gcatagtacg cacgggaata ctcatcccca
    20101 gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc tgcggttcag
    20161 tattgctggc gtgttccggt tgcgaagtga accagcgtac cgcgcggtga cgattttctc
    20221 gttgcgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc gttggtacat
    20281 tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc agcacgcgag acatacatcg
    20341 cctgttcaac catcgacgag gagttgtgcg ccagtcgcag caagtaacca acttccagtg
    20401 cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggaaattc ccctcgctga
    20461 ctaatgcatc cgatacgcca ttgactggca gggtgcgccg tcggttgagt tcgatttgcc
    20521 aggcgtgctc gtcgcgatgc tgttcgaact cggttacggc attacgcaga gaggtgaaat
    20581 ccagtggcgc cacgagagct tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt
    20641 tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta cgctagctgg
    20701 agatataggc atcttccagc accaccactt cacgccaggt gagcaaggtg gaaaaaagca
    20761 ataccacgat aaagcagaga ttaacgacat gaccgggccc caggcggcgt gcgagttttt
    20821 tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact cccaacctgc
    20881 ttttttatga ttctggcata acccggcaat agcgtaccgc aaaacagcga taattttata
    20941 atggtggcat gataagacgc gactatcatt cattgcaggc attgggacac caggccggat
    21001 gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac gactcccctc
    21061 ccccggaaca agatcggttt catgaaaagc ttcccgcttg accccgtaac cgtcatacca
    21121 acgacactca accataccgc tggagtatcc agtgacaatc atccgcgggc cgccctcttt
    21181 aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc gagtgaaact
    21241 tgttcacctt agttgaagat ggcgaatttt gcataatagc cagtgcgagt attaatgtgc
    21301 ctgaccgcgc attttcgcta ctgctttcac tcccagcacc acgatgccgc cgatgataaa
    21361 tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac tttgctgccc
    21421 ggcgatatgt tcaatagcgt gatgcagcgg cgcaatacca tgtaccacaa tcccgccgcc
    21481 gacgaggaac atcgccagcg tgccgacaat cgataatgct ttcatcagcc aaggcgcgat
    21541 aatcaataac cctttaccta atgcctgcat cagcgcgcta gatttttccg ccagccaata
    21601 ccctaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca cggtcactac
    21661 cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcctctg ccacaatccc
    21721 cagcgtgatg gcgacgattt ccgcagacaa aataaaatcg gtacgaatcg cccctttaat
    21781 tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac gctgctggct
    21841 ctgcgctgga tcttctttat gtttacgcgc ctccagcata tgcagcactt tctctacccc
    21901 ttcaaagcag agaaacgcgc caccaatcat caacagtggc gtaatcgccc acgggataaa
    21961 cgcactgatg atcagcgcca gcggcaccag aatcacttta ttgatcagcg atcctttcgc
    22021 cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt gttgcgcatt
    22081 gagcgataaa tcatccccta atacaccggc ggttttcttc gccgccagtt tgcccatcac
    22141 agagatatcg tccagcagtg tggcgatatc atcgagcaac gtcagtaaac tactcccggc
    22201 caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca aaaacggcag
    22261 ctctgatact tgcttcacag gtcaacattt atttaacaat caagtgcaat tatatctctc
    22321 gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccgc cgtgagggat
    22381 tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt atatcatttg
    22441 gggctcaacc tattttgtca ttcggattgg cgtgggaagc tggcctccgt taatgatggc
    22501 gggcgttcga ttccaggcag ccggtatttt attgctggca tttttgctac tgcgcggaca
    22561 caaacttccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt tattgctggc
    22621 tgtcggtaat ggcatggtga cggttgccga acatcaaaat gttccttccg gcatcgccgc
    22681 cgtagtgatt gcaaccgtgc ccctctttac cctgtgcttc agtcgcctgt ttggcattaa
    22741 aacgcgcaaa ctggaatggg tgggtattgc cattgggctt gccggaatca tcatgctcaa
    22801 tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa tcggctcgat
    22861 tagctgggcg tttggctcag tttatggctc gcgcattacc ttacctgtag ggatgatggc
    22921 gggtgcgatt gagatgctgg cggcaggcgt ggtgttaatg atcgcatcga tgattgcggg
    22981 tgaaaaactg acggcgctcc cttccctgtc aggcttcctt gcggtcggct atctggcgct
    23041 gtttggttcg attatcgcca ttaacgctta tatgtattta atccgtaatg tcagtccggc
    23101 tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg gtacgggact
    23161 gggtggcgaa acgctgtcga agattgaatg gctggcgctc ggcgtaattg ttttcgcggt
    23221 ggtactggtc acgttgggaa aatatctctt cccggccaaa cccgttgtcg cgccagttat
    23281 tcaggatgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg ctggcacctt
    23341 cgccgcagat ccactcttcc agacgctcgg taagcgccgc atccgtcagc ttctcgcgcc
    23401 tacgtaacgc acactcccag acaatcagta cgcgccagcc gagctcctgc aagcgactga
    23461 tatcgcggcg atcgcgttca acatttttac ctatcttctc cagccagaac tcagttcgag
    23521 tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg tgggtaaata
    23581 tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg gcgtcctgaa
    23641 cgcgaaacgc aaggccctgc ccggttaaca aactggcgag gcgcttctca atcgccgtat
    23701 cacgcgtggc aatcgcgcgc atatttttgc tgcgggtggc cttatcgtga acgtcgacca
    23761 tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc aagcagtttt
    23821 gccaccgcgg caaagaccgg cacgaccacc gagttgccga actggcgata ggcctgagtg
    23881 tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat taagcgcgcg
    23941 cattcacgag gcgttaaccg acgtggacga tgttgctgat tcagcggatc gtcaaagtct
    24001 ttctcacccg tggccatatc ccaaccgcga tcgattaaaa tttccgcgcc atctttgtaa
    24061 taacgagcag acagcgtacg ggtgacgctt tgcggattgt tcggataaac cattccataa
    24121 ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta cttccacagc
    24181 accggcgtca ggatatattt cgcctcaacc atcgggtcca gcagctgcgc cagcgtcact
    24241 cgctgtgcag ggaaacattc gctgatatca cgcagagtaa aatcggcttt cagattcaga
    24301 tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggcaggaa atgtttacca
    24361 tcgatgattt tcggatcgtc cggcccgtta tcttctgcat cggcaacgtc atagcccagt
    24421 tcgtccagcg tctgcatgat gatgcggaac gtttttccct tatcgtgact cttcaggttt
    24481 ttgacgtttt cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg caccacatca
    24541 aagaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag cgagttcttt
    24601 ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa aacatcgtgt
    24661 tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc ttcttgatga
    24721 ctgagggtga tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc gcaataatgg
    24781 ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac gcactgtcca
    24841 ccaatcgatt caaaaccacg acggatgccg ccaattccgg caaatagatc gataaagcga
    24901 aacgcataat gcggatgatg ttccggtggt ttgggtaaca acgtttgcag atgggcaaac
    24961 tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt taaaattgcc
    25021 gcgctccagt gattctcacc cacaccatta agctgcgcca ccagcatttt aacatcataa
    25081 atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta tgaatcggtt
    25141 actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac aggttaacat
    25201 aattactgcc gtttagggcc acgcgctcag ccagcgggtg aaaacgcatc tatcaccttg
    25261 tgatcgatac cttcattctc ccccgccagt tcggcaccga gcttcgccat caaacagcgc
    25321 caagctcaat gcccctgaaa cggcaaatac gcgcgccagg ccaatcgccc ccagggcttc
    25381 cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga tttgcgcgct
    25441 gaaactgtga gcggcaatgg catgacaaac ggcctcaatt ttctccgccg gaaattgcac
    25501 gaactcttca cggagcaggc gacgcgtctc ttctgccgcc aggattgaag aacgctgccg
    25561 ttgcggatga tttttcgcca ggctcacgat atcgtgaaaa taacaggcgg ttaaaatcac
    25621 cagcatatcg acatcgtcat ctgccgccag tttctgtgcc gtagcccaga cacggcgaaa
    25681 atgacacagg tcatgcgccg cgtcctgatg ttgatgatgg tttttcaacc agttctcgaa
    25741 ctatgcctgc cagtgttgta actccatacg cgctccgttg ccaaagagtc tcaaccttag
    25801 cagtttttat tcatagcgtc tgcaacgtgc ggaacgaggt cgggtgtacc atgcaatgcc
    25861 gcctaacaag gcaccaacca gggccataaa cagaaatccg accaatgctc ctaaccactt
    25921 ccccgcggta gatcctaaat cactttcaaa tcccgacacg ggggcatcgg ggatccggct
    25981 catcacccag atgtatcgcg ccattgccca gacgaaatag ccacaccatg tatagaaagc
    26041 ccac
//
LOCUS       DAJTRM010000056        25645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRM010000056 DAJTRM010000000
VERSION     DAJTRM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.056"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO322_002416"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO322_002416"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5907258.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO322_002417"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO322_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5907259.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO322_002418"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO322_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5907260.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO322_002419"
     CDS             3807..3995
                     /locus_tag="OO322_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5907261.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO322_002420"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO322_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5907262.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO322_002421"
     CDS             4756..5115
                     /locus_tag="OO322_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5907263.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO322_002422"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO322_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO322_002423"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO322_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5907264.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO322_002424"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO322_002424"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5907265.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO322_002425"
     CDS             10913..11254
                     /locus_tag="OO322_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5907266.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO322_002426"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO322_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO322_002427"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO322_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5907267.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13680..14966
                     /gene="dctA"
                     /locus_tag="OO322_002428"
     CDS             13680..14966
                     /gene="dctA"
                     /locus_tag="OO322_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5907268.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15187..16683
                     /locus_tag="OO322_002429"
     CDS             15187..16683
                     /locus_tag="OO322_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5907269.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16779..17708)
                     /gene="kdgK"
                     /locus_tag="OO322_002430"
     CDS             complement(16779..17708)
                     /gene="kdgK"
                     /locus_tag="OO322_002430"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5907270.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17940..18707
                     /gene="pdeH"
                     /locus_tag="OO322_002431"
     CDS             17940..18707
                     /gene="pdeH"
                     /locus_tag="OO322_002431"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5907271.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18777..20837
                     /locus_tag="OO322_002432"
     CDS             18777..20837
                     /locus_tag="OO322_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5907272.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21019..22341)
                     /locus_tag="OO322_002433"
     CDS             complement(21019..22341)
                     /locus_tag="OO322_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5907273.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22752..23765)
                     /gene="yhjD"
                     /locus_tag="OO322_002434"
     CDS             complement(22752..23765)
                     /gene="yhjD"
                     /locus_tag="OO322_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5907274.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23814..24713)
                     /locus_tag="OO322_002435"
     CDS             complement(23814..24713)
                     /locus_tag="OO322_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5907275.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25233..>25645
                     /locus_tag="OO322_002436"
     CDS             25233..>25645
                     /locus_tag="OO322_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5907276.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIK"
BASE COUNT         6102 a         6445 c         6980 g         6118 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccccca taaccttaca agacctgtgg ttttactaaa ggacacccta
    13681 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    13741 ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct
    13801 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    13861 ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact
    13921 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    13981 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    14041 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg
    14101 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    14161 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt
    14221 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgc
    14281 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    14341 agctgattat ctgtttctac attacctgta tcctgtttgt ggtgctggta ttgggttcaa
    14401 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc
    14461 tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    14521 agaaactcgg ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    14581 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta
    14641 acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta
    14701 aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    14761 tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag
    14821 aagctcgtgc gctaactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    14881 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    14941 gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag
    15001 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    15061 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    15121 acgtcattta gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg
    15181 gttcacatgc agggcataaa aattcgactt ttagcgggcg gtttgctgat gatggccact
    15241 gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt
    15301 tccaacggtt tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa
    15361 attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt
    15421 cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt
    15481 tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat
    15541 gacaccacgc tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg
    15601 ctctcttatc tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac
    15661 gcgctgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg
    15721 cgctatcgtc tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa
    15781 cccgttgaag cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg
    15841 acgctactgg tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg
    15901 tttggcgaac tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg
    15961 cgtgcggaag cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg
    16021 tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg
    16081 gacctggccc gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtgacagc
    16141 aaagacatcg gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc
    16201 aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg
    16261 gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag
    16321 aaactggagc tgcagaaact gtttgccgcc tatgcacggg ctgataccga tattctgatg
    16381 ggccagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag
    16441 aaactgcggc aggatttcct taacagcctg actgtggaga tgttaaatca ggatctgcgt
    16501 cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt
    16561 aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatctac cgccgctgcg
    16621 accacctctg tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag
    16681 taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga
    16741 ggggtaacac tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc
    16801 gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc
    16861 gcgttttccg cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg
    16921 ccagctgcgg tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatcaact
    16981 aacgcttcac cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct
    17041 ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc
    17101 gcgtcttcgt cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgctggtac
    17161 acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc
    17221 acttttccgc ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc
    17281 gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct
    17341 tcgcaaatcg ccgcagactg ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc
    17401 cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga
    17461 cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc
    17521 atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca
    17581 ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc
    17641 ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt
    17701 ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt
    17761 tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg
    17821 aaagattccg ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgccctta
    17881 cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga
    17941 tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg
    18001 aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat
    18061 gcgggcggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc
    18121 aacgcctgcc gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg
    18181 tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacggcctgc
    18241 tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc
    18301 tgcgccagat tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc
    18361 cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg
    18421 gtaccgggat ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg
    18481 cgcgagaact gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc
    18541 ttttacatct aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg
    18601 aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac
    18661 gcccggcacc gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt
    18721 ccgccgggct ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga
    18781 gcaaggcagg caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg
    18841 cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag
    18901 tctctgcgga gttgaatcgt ccgttcgcta tccgtggcaa tctgggcgtg gtgtgggagc
    18961 ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag
    19021 acatcattct tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag
    19081 tagaggcaac gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca
    19141 agctcgaaaa gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt
    19201 ttaatcttgc caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc
    19261 ggctggataa tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag
    19321 cggatctgga aatttttgtt gatccgttag gcaagccgct gccattcagc gaagttactg
    19381 gatcgaaagg taaagcggat aaagaaaagg tgggcgatta catttttggc ctgaaggcgc
    19441 agggacgtta taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc
    19501 tgcgtggcga agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg
    19561 ttgcttttga tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta
    19621 aattttctgg cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata
    19681 ccccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg
    19741 tctttgatta tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg
    19801 tctacaccac cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc
    19861 ggctggcgga cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga
    19921 aacggtctga acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt
    19981 atgaccgctt cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc
    20041 ggcgcattga gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca
    20101 aaaatgctga cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg
    20161 cgaatattca tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc
    20221 gtcgactgaa actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg
    20281 aaatgaacgg tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca
    20341 acagtaacgg caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg
    20401 agattgttgg gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg
    20461 tgcgggtgaa ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga
    20521 tttttgcttt cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt
    20581 cggaacagct ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc
    20641 gttcgccgct gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac
    20701 cgctgattgc ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg
    20761 cgttactggc actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt
    20821 cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt
    20881 agatttttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    20941 tacaaatcct tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca
    21001 tcaggctttt cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc
    21061 gattaacgtc aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt
    21121 agcctgcaac caggctgcga tatacggcgc aacggaagcc ccgagaatcg acgatacgtt
    21181 gtaagagaac gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc
    21241 acccatcggc ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac
    21301 cagaatcggg ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat
    21361 cagcgtggtg atgatcacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc
    21421 gactggcacc atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt
    21481 acgcggcagg ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt
    21541 catgatgtaa aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg
    21601 aacatgtttg gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac
    21661 tttctcaaac accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag
    21721 caccgccgag aagataaatg gcacacgcca gccccagctc ataaactgct catcggtcag
    21781 tagccaggaa agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag
    21841 ctgtgggaag gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag
    21901 cgccgcgccg ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag
    21961 cagcagtggg gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt
    22021 cgaaatcccc atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc
    22081 aaaatgacca aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc
    22141 gagcgactgt agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac
    22201 aataacggcc gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag
    22261 agaggcgaca aggactttat tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag
    22321 tgttgtggct gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta
    22381 cgcacagcaa aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc
    22441 aaaaagggga taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt
    22501 ataaatagtg gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc
    22561 tggggttatg agttggagtg aaattgtttg ctcccaaata gatggctgaa gaggctctgg
    22621 ggaagagttt caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat
    22681 ccggcctacg gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca
    22741 tccggcctat tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg
    22801 gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc
    22861 agccccagca cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg
    22921 taggtcatca cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc
    22981 agggcttttt tgcgcggacg gtggcgcggc aaccgccaga agatccagaa gaaaagcaga
    23041 tagttggcga agatggaaat cgccaggcca atcaatcgcc acgtcggttt cagccactca
    23101 atgctgttca ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg
    23161 atcgaaagcg tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga
    23221 tattttaccc agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg
    23281 gcacgaatcg cttcacgcag gttacccatc cagttgatgc cggaataaag tgccaccgcc
    23341 aggccgacaa gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac
    23401 gtggcggcta gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc
    23461 aacatcggat gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg
    23521 atcatcgaca aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg
    23581 ttaaagcgtt ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg gactttttcg
    23641 gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc
    23701 aactgcttaa tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc
    23761 gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc
    23821 aggtgctctt tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc
    23881 tcccgacgct gcgggtaaac cagcgaaagg gagagcggct cggcacggta gcctggcaac
    23941 acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata
    24001 atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg
    24061 ccgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt
    24121 acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc
    24181 ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt tagtttgccg
    24241 aggggacggg cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa
    24301 ccatcatgaa gaatatctac cggacggtta tggctactca gttccagctc aattcccgga
    24361 tgctgataga gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg
    24421 atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt
    24481 tcgctgagat tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg
    24541 agtttgaccc gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc
    24601 tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa
    24661 ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg
    24721 accgattgtt gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct
    24781 aacatctaat gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga
    24841 aaagggcgga atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt
    24901 gattcccttt aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg
    24961 ccatttgtta ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc
    25021 accaatataa ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg
    25081 cgtttagctg taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg
    25141 ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag
    25201 tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt
    25261 caatatttat tcatggaata aaaatcagtt tacagcagcg tattccagga gtgagtattc
    25321 agggggtcag tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag
    25381 ttatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg
    25441 tgcagtttcc tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac
    25501 aggaagttat ccattttaat gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat
    25561 ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga
    25621 cgacgccgga aaaggacatt aaaga
//
LOCUS       DAJTRM010000057        25291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRM010000057 DAJTRM010000000
VERSION     DAJTRM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.057"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1247)
                     /gene="yeaG"
                     /locus_tag="OO322_002437"
     CDS             complement(<1..1247)
                     /gene="yeaG"
                     /locus_tag="OO322_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5907277.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEG"
     gene            1683..2429
                     /gene="mipA"
                     /locus_tag="OO322_002438"
     CDS             1683..2429
                     /gene="mipA"
                     /locus_tag="OO322_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5907278.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2519..3280
                     /locus_tag="OO322_002439"
     CDS             2519..3280
                     /locus_tag="OO322_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5907279.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3331..4215)
                     /locus_tag="OO322_002440"
     CDS             complement(3331..4215)
                     /locus_tag="OO322_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5907280.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4299..5294)
                     /gene="gapA"
                     /locus_tag="OO322_002441"
     CDS             complement(4299..5294)
                     /gene="gapA"
                     /locus_tag="OO322_002441"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5907281.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5636..6049
                     /gene="msrB"
                     /locus_tag="OO322_002442"
     CDS             5636..6049
                     /gene="msrB"
                     /locus_tag="OO322_002442"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5907282.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6091..6363
                     /locus_tag="OO322_002443"
     CDS             6091..6363
                     /locus_tag="OO322_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5907283.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6733..7809
                     /locus_tag="OO322_002444"
     CDS             6733..7809
                     /locus_tag="OO322_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5907284.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7836..9215
                     /locus_tag="OO322_002445"
     CDS             7836..9215
                     /locus_tag="OO322_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907285.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9232..10277
                     /locus_tag="OO322_002446"
                     /pseudo
     CDS             9232..10277
                     /locus_tag="OO322_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10298..11134
                     /locus_tag="OO322_002447"
     CDS             10298..11134
                     /locus_tag="OO322_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5907286.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11139..12086
                     /locus_tag="OO322_002448"
     CDS             11139..12086
                     /locus_tag="OO322_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5907287.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12096..13076
                     /gene="ydjG"
                     /locus_tag="OO322_002449"
     CDS             12096..13076
                     /gene="ydjG"
                     /locus_tag="OO322_002449"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5907288.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13213..13971
                     /locus_tag="OO322_002450"
     CDS             13213..13971
                     /locus_tag="OO322_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5907289.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14089..15447
                     /locus_tag="OO322_002451"
     CDS             14089..15447
                     /locus_tag="OO322_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907290.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15540..16181)
                     /gene="pncA"
                     /locus_tag="OO322_002452"
     CDS             complement(15540..16181)
                     /gene="pncA"
                     /locus_tag="OO322_002452"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5907291.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16192..17208)
                     /gene="ansA"
                     /locus_tag="OO322_002453"
     CDS             complement(16192..17208)
                     /gene="ansA"
                     /locus_tag="OO322_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5907292.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17375..19231)
                     /gene="sppA"
                     /locus_tag="OO322_002454"
     CDS             complement(17375..19231)
                     /gene="sppA"
                     /locus_tag="OO322_002454"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5907293.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19392..19943
                     /locus_tag="OO322_002455"
     CDS             19392..19943
                     /locus_tag="OO322_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5907294.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20060..21103
                     /gene="selD"
                     /locus_tag="OO322_002456"
     CDS             20060..21103
                     /gene="selD"
                     /locus_tag="OO322_002456"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5907295.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21108..23069
                     /gene="topB"
                     /locus_tag="OO322_002457"
     CDS             21108..23069
                     /gene="topB"
                     /locus_tag="OO322_002457"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5907296.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23196..24236
                     /locus_tag="OO322_002458"
     CDS             23196..24236
                     /locus_tag="OO322_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5907297.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24353..>25291)
                     /locus_tag="OO322_002459"
     CDS             complement(24353..>25291)
                     /locus_tag="OO322_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5907298.1"
                     /translation="KGKSEGKVMRFCQTLMTELYRHLGADTDVPAGDIGVGGREVGFM
                     AGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLKRHGMGFEGMSVSV
                     SGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRAAD
                     YAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGAYMPTTI
                     EATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHH
                     ACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         6424 a         5873 c         6523 g         6471 t
ORIGIN      
        1 atcccttcat cgacaccggc gtagtcacga tattcctgat acgacttggc tttcggatcg
       61 gtgtctttca gactttcgcc atcataaacc cgcatctttg aataaatgct ggagttttct
      121 ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg tttcgagcgt gccaggggcg
      181 catggggcgt gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg
      241 gaaatgcgca agcaatacgg caccttcacg atgtaaacac ggtcgaggaa ggcttcgttg
      301 tttttgttat tacggaaagt gacccattcg gacttgttcg agtgggcgag aataatcccg
      361 ttgaacggca gggcggagat accttccgtc ccgttgtagt taccttcctg agtggcggtt
      421 aacaagggat gcagcacttt tatcggtgct ttaaacatct caacgaattc catgatcccc
      481 tgattggcgc ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag
      541 tgttcgagtt tacgaatatc gactttccca accagcgcgg agatgtcctg gttgttctca
      601 tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa ttgacggcca gaccttcact
      661 acccggaact tagtgatatc gccaccaaat tcatgcaggc gttttgccgc ccatggcgac
      721 atgatggtgc cgagataacg gcgaggaatg ccatactctt tttccagaat ctgagcatct
      781 tcctgcggat tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttggcg
      841 ctcaatacat aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat
      901 ttacccccac ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga
      961 gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt cttccatgcc gtaaaactct
     1021 tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctggggtcc
     1081 tgggctgtat cgaccatgac aggctcaccg atagccatca atagccgctc agccgcgttg
     1141 gcataagcac tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg
     1201 tccttggcag cttcatatcg ctggcgatag tgatcgaata tattcatggt atgccgtcct
     1261 ttcgtttttt agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg
     1321 taaactgaac gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg
     1381 agaacaacgt gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt
     1441 gcctgcacgg ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata
     1501 attaatgtaa agaccaggct cgccagtaac gctaaatcca tttggctgta agcgcggtgt
     1561 catccgcggc aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt
     1621 ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa
     1681 ttgtgaccaa actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag
     1741 cgcacgctga aggtaaattt tccctgggcg caggcgtagg tgtcgttgag cacccatata
     1801 aagattacga taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct
     1861 ggttccgtgg cttaggtggt ggttactacc tgtggaatga cgcaacggat aaactttcaa
     1921 ttaccgctta ctggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc
     1981 gtcacctgga tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc
     2041 agtacggtta cctgcgtacc accctggctg gcgataccct ggataacagc aacggcattg
     2101 tctgggatat ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta
     2161 ttggtgtgca gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag
     2221 agtccgctcg cagcggtctg cgtggctata acccgaatga cagctggagc ccttaccttg
     2281 agctgagcgc cagctacaac ttcctcggcg actggagtgt ttacggtacc gcgcgctaca
     2341 cccgtctgtc tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga
     2401 tttctaccgg gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata
     2461 aaaacagggc gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat
     2521 gcaacaaaaa atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac
     2581 atggtatatg ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcagg
     2641 cattgaactc ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga
     2701 aaaggtggtt ggggaagcat taaccggtct gcgagagaag gtctttctcg tctctaaagt
     2761 ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg
     2821 tctcaatact gactatctcg atctctactt attacactgg tctggcagtt tcgcttttga
     2881 agagactgtt gctgcgatgg aaaaattgat cgcccaggga aaaatccgtc gctggggcgt
     2941 ttctaacctt ggttatgccg atatgcagga actctggcag ctgccggggg gaaatcagtg
     3001 tgccactaat caggtgcttt accatctcgg ttcacgaggg attgagtacg atctactccc
     3061 ctggtgccag caacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg
     3121 gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa attgcacatg tccaacaaca
     3181 tgcagctgcg cttgaggtcg aactttcgtc agcggaatta gctatgctgg ataaggcata
     3241 tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc
     3301 atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg gcaacgcgaa tggattgcgc
     3361 cagatgtgca ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac
     3421 acaaacaaag gtcttgtagc catcgtccgg catgtcgccc atgctaactg aaagcgccgg
     3481 tcccgggttc cagccgacaa cgttcagatg atgctggtgg cctacggcga taatacgatt
     3541 cagcgcttca tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg
     3601 gtcagggaag gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat tcactttatc
     3661 aatgaagcga tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta
     3721 ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat tcaagatcga tttcacaggt
     3781 tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc
     3841 ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca gcatcttcat gatgtgattt
     3901 cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt gtgccgccgg
     3961 accaaaccac ggccagcaaa ccggtacgcc accgcgaata gcgacgccat ttttgaacgg
     4021 tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca ggtttccacg agagaaggtg
     4081 tgcgccctgt aatgcaaaag aggcttttac ctggggatga tcgaccacaa tgaggtccag
     4141 ttcatccagt ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa
     4201 aattttctta atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga
     4261 ccgaagtcgc tctttttaga tcacagtgtc atctcaactt atttggagat gtgagcgatc
     4321 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
     4381 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
     4441 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
     4501 tcagcagcag ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
     4561 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
     4621 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
     4681 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
     4741 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
     4801 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
     4861 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
     4921 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
     4981 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc cacttcgtcc
     5041 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
     5101 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
     5161 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
     5221 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     5281 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     5341 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     5401 tgtgcctaag cattacgcga ctgactcgcc tcacttttcc tttgggcttg agaccacatg
     5461 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     5521 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     5581 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agtaaatggc
     5641 gaataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     5701 gcagaatcat gggacagaac cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     5761 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc
     5821 cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     5881 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     5941 ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc
     6001 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     6061 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     6121 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc
     6181 gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc
     6241 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     6301 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc catgtttaag
     6361 taagtggttc atcccctttt tgccgcggct atgaagggga tagccaacaa tgaccccacg
     6421 actcgtctca attatctcga cccgacctgg ctaagtccca gtccgatctc ttgcagtcgc
     6481 gttcacgcct gaatgacttc tcctgaactt aagtctcaga cctatttggc cggtaatccc
     6541 tctcgaattt gctctttttg cgtcccgcct tccaccaacc tgtaacagac ccagccattt
     6601 tctgcgtaat tgtgatttga gttgtaaaat gcaggtcatt atctgtgttg tgcagattta
     6661 atattgacct caaaaatcat gtgggtaagt atgatggcca tctaagcaaa acaaaacaca
     6721 gaggatgacg caatgaaagc actggctcgg tttggcaagg cctttggcgg ctacaagatg
     6781 attcatgtcc cacaacccat ttgtggcccg gaagacgtag tgactgaaat taaagccgcg
     6841 gcaatctgcg gcgcagacat gaagcactac aatgtcgata gcggttctga tgagtttaac
     6901 tctatccgcg gccatgagtt cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac
     6961 tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc acgtttgcgg cgtttgtccg
     7021 gcctgtgaac agggtgattt tctgtgttgt acagaaaaag taaaccttgg tctggataac
     7081 aatacctggg gcggtggttt ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt
     7141 catcgtcatg cgttgtggga aatccctgat ggtgttgatt atgaggacgc agccgtactt
     7201 gaccctatct gtaatgccta taaatccatc gcgcagcaat cgaaattcct tcctggtcag
     7261 gatgttgtcg tcattggcac tggcccactc gggctgtttt ccgtacaaat ggcgcggatt
     7321 atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg
     7381 gttgcaaaag aactgggggc gacggcagta gtaaatggtt ctaccgaaga tgtggtggcg
     7441 cgctgccagc aaatttgtgg caaagataat ctgggactgg tgattgaatg ctccggtgcc
     7501 aatatcgcac tgaaacaagc catcgaaatg ctccgcccga atggggaagt ggtacgcgtt
     7561 ggaatgggct tcaaacctct tgatttctcg attaatgaca ttaccgcctg gaataaaagc
     7621 atcattgggc atatggccta tgactccacc tcatggcgta acgctatccg gctattagcc
     7681 agcggcgcta tcaaagtcaa accgatgatc acgcatcgta tcggcctgtc gcaatggcgc
     7741 gaagggtttg atgcgatggt cgataaaacc gcaatcaaag tgatcatgac ttacgacttt
     7801 gatgaataac tactctcctg aaggaacgtt atcgcatgga acagataaca aaaccgcatt
     7861 gtggtgcacg gctggatcgc ttaccggatt gccgctggca ttcgtcaatg tttgccatcg
     7921 tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg tggcttgatc ctcgcgcagc
     7981 tgaaagcgtt gggctggaca gataattcca ctacagccac attctcagca atcacgaccg
     8041 ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa
     8101 atgcgttcat cctctatgag gccattcata ttgcctcgat ggttgtcggt gctttctcac
     8161 cgaatatgga ttttcttatt gcctgccgtt ttgtgatgga cgttgggctg ggagctttac
     8221 tggttacact gtttgctggt ttcaccgaat atatgcccgg tagaaatcgc ggaacgtggt
     8281 ccagtcgggt ttcctttatt ggcaactggt catatccgct ctgctcactg attgcgatgg
     8341 gactcacgcc gctgattagt gcagagtgga actggcgggt acaactgctt attcctgcaa
     8401 tattgtcgct tatcgctacg gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc
     8461 tggaatcgcg cggacggtat caggaagccg agaaagtcat gcggagtata gaagaaggcg
     8521 tcatacgcca gacgggtaaa cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc
     8581 cacaagcggt gccgtattca gccttactga caggagtatt actgaaacgc gtgatattag
     8641 gctcttgtgt gctgattacc atgaacgttg tgcagtacac actaattaac tggttgccaa
     8701 caatattcat gacccagggg attaatttaa aagactcgat tgttttaaat accatgagta
     8761 tgtttggcgc gccatttggt atttttattg ccatgctggt gatggataaa attccgcgta
     8821 aaacaatggg tgtggggcta ttaatcctga ttgcggtgct cggatatatc tattcactgc
     8881 aaaccagtat gttgctcata acgctgattg gtttcttcct gattactttc gtctatatgt
     8941 acgtttgcta tgcctcggca gtgtatgtcc ctgaaatctg gccgacagag gccaaactcc
     9001 gtggctccgg tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg
     9061 ttgcagtgct gctcagtagt tatggtgtaa cgggagtctt tattcttctg ggggctgttt
     9121 caaatattgt cgcaattgct atcgccacca ttggaattga aaccaaaggt gtctccgttg
     9181 aaagtttaag tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat
     9241 tcaaaagcaa tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg
     9301 cctaaagaag atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta
     9361 catggttttt aatcaggccc gtttattccg cctaaagacc caaatcaaga aattggcctg
     9421 ggtcatgaat gcgccgggac ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg
     9481 ggggatcggg taaatatcga acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa
     9541 ggcaaatata acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc
     9601 gcattaacgc actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg
     9661 gacacgatgg aaggggcgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg
     9721 gcagatgtta aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg
     9781 acgttgcaag cgtgcaaatg cctgggcgca acggaaattg ccgtcgttga tgtactggaa
     9841 aaacgtctgg caatagggga acagcctggt gcgacagtgg ttattaatgg cgcaaaagag
     9901 gacactattg cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa
     9961 acagcgggtt ctgcggtcac cgttaaacag gcacctttaa tctggtaatg cgcggcggta
    10021 aaattatgat tgttggtact gtacccggcg attcggcaat caatttcctc aaaatcaatc
    10081 gcgaagtcac tatccagacg gtattccgct atgccaatcg ttatccggtc acgattgaag
    10141 ctatttcttc agggcgattc gatgtgaaat cgatggtgac gcatatttac gattatcggg
    10201 atgtacaaca ggcatttgaa gagtcagtta acaacaaacg cgacattatt aaaggcgtta
    10261 ttaaaattag cgattaatta atacgggaga acagaacatg ctcgcagata tcagatattg
    10321 ggaaaacgat gccacaaata aatattatgc aattgcccat tttaacgtat ggaatgcaga
    10381 aatgttgatg ggcgttaaag acgcagccga agaagcgaaa tccccggtta ttatttcttt
    10441 tagtacaggt tttgtcggta acacctcatt tgaagatttc tctcacatga tggtatcaat
    10501 ggcacaaaaa gcaacggtgc cggtaataac tcattgggat catggtcgga gtattgagat
    10561 tattcataac gcctggactc atggtatgaa ttcattaatg cgtgatgctt ctgcatttga
    10621 tttcgaagaa aatattcgtt taaccaaaga ggctgttgat ttctttcatc cgctgggtat
    10681 tccggtagag gcggaattag ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc
    10741 gggctatcac tataccgatc ctgaccaggc ggctgaattt gttgaacgta cgggctgtga
    10801 ctcattggct gtcgccatcg gcaaccagca tggggtttat acgtcagagc cgcaattgaa
    10861 ctttgaggtc gtcaaacgcg tacgcgatgc tgtttctgtt ccgctggttt tgcatggcgc
    10921 atcggggatc agtgatgccg acattaaaac tgcaatttcg ttgggtattg cgaaaatcaa
    10981 cattcatacg gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc aggatcagcc
    11041 tttcctgcat ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat
    11101 tgaactgttc ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt
    11161 gtataggtgc cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg
    11221 tagattctta tcctcttgaa agaatcgcaa tgagcaccgg gggcgatgca ataaatgaag
    11281 caacaattat ttctcgtctg gggcatcgta ccgcattaat gagccgcatt ggtaaagatg
    11341 cctcagggca atttattctc gaccattgtc gcaaagagaa tattgatatt caaagcctga
    11401 aacaggacgt cagcatagat acatctatta acgttggact ggtgacggag gatggcgagc
    11461 ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt aaatattgac gatgttgatt
    11521 ttgcgcggtt tcctcaggcg aaattattat cactggccag tattttcaac agtcctctat
    11581 tggatggtaa agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt
    11641 gtgccgatat gatcaaaccg cggttgaatg aaacgctgga tgatatttgc gaagcattga
    11701 gttatgtcga ttatctgttt ccaaattttg ccgaggaaaa attactcaca gggaaagaga
    11761 cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa
    11821 cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg
    11881 caggaataac cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg
    11941 cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg
    12001 ctatctcggt tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac
    12061 aattgctgga agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacga
    12121 cggatataac gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat
    12181 ggaatggcga tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg
    12241 gcattaatct gattgatact gcgctaggat ataactttgg caatagtgaa gttatcgtcg
    12301 gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg
    12361 tctgggaacg aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc
    12421 tttccccgga atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt
    12481 acatcgatat ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg
    12541 aaactgtcgc agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg
    12601 ctaacgtcga tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc
    12661 aggcgaaata cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg
    12721 ataatggcat tgtggttcag gtttattccc cgctagagca gggattgttg accggcacta
    12781 tcactcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa
    12841 acatgctgaa agtgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt
    12901 gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc
    12961 ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact
    13021 tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac
    13081 tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg
    13141 ttattttgaa agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac
    13201 aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac
    13261 gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt
    13321 cgccgcgatc ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct
    13381 gttttaaata cagcggtgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt
    13441 tatgaagaga agctgcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc
    13501 atggcagccg attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt
    13561 gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt
    13621 aaagtcgttt caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt
    13681 actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt
    13741 gatattaaca gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg
    13801 atccgtcagg cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct
    13861 tttgtccagt tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt
    13921 gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac
    13981 acgttgtcaa tattatttgt tcctgcgact ggaaataaaa tgatgataaa gatgcggcaa
    14041 gtgaatttgc cgcaattgct accttttata gcaaatccga ggcacccaat ggaacaatat
    14101 gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt
    14161 ggtataataa gctttagtct gttattaacg gggtttttga gttactcagg taatgtcgtc
    14221 ttagcaaagc tggtaagcaa tggatggtca aataatttcc tctatgccgc ctttacctcg
    14281 gcattaatgt ttggttattt catcggctca cttactggtg ggtttattgg tgactacttt
    14341 gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc aacaggggcc
    14401 gcttttgtcc ctgatatgta ctggctcatt ttctttcgct tcctgatggg aacaggaatg
    14461 ggggcgctga ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt
    14521 ggaaaatggt ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct gtctgcggcg
    14581 ataggcgtgg tggttatcgc tttttttagt tggcgaataa tgtttcttct gggtggtatt
    14641 ggcatactgt tagcctggtt tctctcaggt aaatacttta tcgagtcgcc acgatggctg
    14701 gaagggaaag ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga gcagcaaatt
    14761 gaaagggaga agagtattcg tttaccccag cttactttga accagagcaa cagcaaggtt
    14821 aaggtaatca agggtacctt caggctcctg tttaaagggg aaatgttacg acgtacatta
    14881 gttgcgataa ctgttttaat tgcaatgaac atttcgcttt ataccattac cgtatggata
    14941 ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt
    15001 attatgattg gtgctccggt aggaatattt attgcggcat taattattga tcattttcct
    15061 cgtcgattat ttggctccgc cttacttatt attattgccg tgttaggcta tatctattca
    15121 agtcagacta cagagtgggc gattttaatc tatggtctgg tgatgatctt ctttttatac
    15181 atgtatgttt gcttcgcgtc ggcggtttat atcccggagc tttggccaac gcatttacgc
    15241 ctgcgcggtt cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt cacgccctat
    15301 ggcgttgcgg cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc
    15361 atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg
    15421 ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcatcaatt gccagatgta
    15481 ccactgacgt gttttatttg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat
    15541 tacccctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatc
    15601 tccataaacg cgtgtgcact gtcctggggc tggatattca cgccacgaca gccatcggta
    15661 atcacgttta ccttataacc taactgtaac gcgtccagca cggtaaactt cacgcaatag
    15721 tcagtagcca ggcccataac gatcaattca tcgatttcat gatcgcgcaa ccagtcatca
    15781 agcgcggttt tctgccgacg tccgttatca aaaaaggcac tgtaactgtc aactaaagga
    15841 ttttcgcctt tatggaacac cgctgcgatc tctttttgat tcagtaacgg atgtaattgt
    15901 gcgccttcac tgttctgcac acagtgttca ggccagaaag tttgccgcag gccgccgagt
    15961 tggcctggcg tataaggctc tacgccgtgc tggctggcaa agctgccgtg attcgccgga
    16021 tgccagtcct ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg
    16081 ttagcaacct ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa
    16141 tcattttgta aatcgaccag taacagggcg cgatggggca ttgcagtctc cttaatcatc
    16201 cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt cagtatccag
    16261 ttcctggctc agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc
    16321 gtcaataacg ccggcatggg cgagggcgtt accggtggca taaccactca tattcacttt
    16381 accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa
    16441 ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccataggagc gcagaatcaa
    16501 tgctttcacc ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ctggataaat
    16561 cgtcaccacg ccaattggtt gtggcgtgat tggatgaacg attaattccc cttcaccgtg
    16621 cggggcgggt ggcgtattca aacgacgaat atggatacca gcttccagta acggaggaag
    16681 gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg
    16741 atacaatcgg ttattgaaaa agagcgttac ttcgttgatc ggataattcg gcgcaacgta
    16801 caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga
    16861 ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg cagaggcggt
    16921 atacgccatc gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc
    16981 tttaatatct tcagcaatat gctgccagtc ttccggcgtc atatcagaag aatccatcaa
    17041 cggcgtatat tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag
    17101 cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat
    17161 cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg
    17221 aatgatatcg aaagagcaaa agtatatacg tgaggcagga ggagcgcacc tgatactatg
    17281 cggataagga aatatggggc gagacagcgg gaagaggatt cgtcgaggta aaaaataatg
    17341 tggccgacga tctgtcggcc actcagtaca agacttaacg cacgttggcg caggtcaggc
    17401 aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa
    17461 cggtagaggc taccgaggca agcggtgcag gtaacatggc ctggaacgca tctggcaaca
    17521 ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa
    17581 cgtagtattc cagatgccac tgtttcactt ttgcgagctc tgctgctttg gcaaccgcat
    17641 catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga
    17701 cgtggccctg ggcgatttta tcaatttgct ccggcgtcga atgacgcgca tcagcaacca
    17761 gcgtgataaa gcgtttatag ccattctcaa tgcttaattg catcatctgc tgcgcttccg
    17821 gcggcagtgc cctggtgata gaaacatccg ccagcggtga agttgagaca ccatcagtat
    17881 gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag
    17941 aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa atccagtaac
    18001 caccagatgc cgccatgccg cccatcgata caaccacaga cttacccgct gcccgggctg
    18061 ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgcctggg ctattaacac
    18121 gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg
    18181 tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga
    18241 cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga
    18301 tggcgcgata atttttatca gtcttactcc agccgaactc tttggtcaga gctttttcga
    18361 tttcggcact cgatgccagt gcatcgacca gcttgttttc cagtgcatat ttcgcggtat
    18421 cgccaccggt tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag
    18481 cagggatctg ccggttagcg gcaacagtat tcagatagtt ttgccacagc tcaccaatcc
    18541 agcggctgtc agcttcgcgg gctgccggtg acatatcatc acgaataaac ggttcaacgg
    18601 cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta tccagcaacg
    18661 atttgtagta cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca
    18721 gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa
    18781 cggcataaac cggtttcccg ctgtcacgaa actctttcag ggctttgccg atgtactgca
    18841 tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacgataccg gtgatattgc
    18901 ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac
    18961 gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtctg
    19021 gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt
    19081 tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagtacg aggaaaataa
    19141 agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa
    19201 atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt
    19261 cacaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg
    19321 cggactgtgc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcacgacag
    19381 gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg
    19441 ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc
    19501 cggaccataa gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa gggtgcgagc
    19561 gtttcagcgc cgtactggaa cagggggcga ttgctgccgg tagtgatgat aaagctatcg
    19621 acaaagcccg taatgcgccg ttccgcgcac cgctcatcat cacggtggtg gcgaaatgca
    19681 aagaaaatca taaagtcccg cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg
    19741 cgatgcaaat ggcagccgtt gcccagggat ttggcggcat ctggcgcagt ggcgcattaa
    19801 ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt
    19861 ttctctacct cggcacgccg cagttgaaag catctacgtc gattaacgtc ccggacccga
    19921 cgccgtttgt aacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc
    19981 gcaggaattc agacactctc acttatcact tcacggaatg agggttacca tacccggatt
    20041 gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg
    20101 agctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca
    20161 ggcgaagttt gttgatccga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt
    20221 gtacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt
    20281 tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatcttcgc
    20341 gatggggggc aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc
    20401 cccggaaatt gcccgcgaag taaccgaagg tggacgctat gcatgccgtc aggcgggtat
    20461 tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac
    20521 ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact
    20581 gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt
    20641 gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc
    20701 gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttacgggct ttggtctgct
    20761 gggccacttg agcgaaatgt gtcagggggc tggtgtgcag gcacgcgtcg actatgaagc
    20821 gatcccgaaa ctccccggtg ttgaagagta cattaagttg ggcgcagtac ctggcggcac
    20881 tgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga
    20941 tctgctgtgc gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga
    21001 aaatgaggtt aaaactacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact
    21061 ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taatttaatg cggttgttta
    21121 ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgga
    21181 aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt atcggtcacc
    21241 tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg
    21301 atttgccgat tgtcccggaa aagtggcaat tacagccacg accctccgtg accaaacaac
    21361 ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg
    21421 atcgtgaagg gcaattgctg gtggatgaag tgctggacta tctgcaactg gcaccggaaa
    21481 agcgccagca ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg gttgagcggg
    21541 cgatcgaccg tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct gcgctggcgc
    21601 gagcgcgtgc cgactggctg tacggcatca atatgacccg tgcgtatacc attctcggtc
    21661 gcaatgccgg ttatcagggc gtactttccg tgggacgcgt gcagacaccc gtgctcgggc
    21721 tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca
    21781 aagcacatat cgtgacacct gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag
    21841 cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg
    21901 tggttaaccg cattagtggt caaccggcta ttgtcaccag ctataacgat aaacgggaat
    21961 cagaatccgc gccgctgccg ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct
    22021 tcggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc
    22081 taatcactta tccgcgttcc gattgtcgct atttgccaga agaacatttt gccggacgcc
    22141 acgcggtgat gaatgccatc agcgttcatg caccggatct gttgccgcag ccagtggtag
    22201 atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca
    22261 tcccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc
    22321 tgattgcccg tcaatacctg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta
    22381 tcgaactgga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag
    22441 gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccattgc
    22501 ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa
    22561 cccagccgcc gcgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcgc
    22621 gctttgtgca ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt ctggggacag
    22681 aagcaacccg tgccgggatt attgaactgc tgttcaagcg tggttttctg accaaaaaag
    22741 ggcgctatat ccactccacc gacgccggaa aagcgttatt ccattcgctg ccagaaatgg
    22801 cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc
    22861 agtgtcgcta tcaggacttt atgcagccgc tggtggggac gctatatcag cttattgatc
    22921 aagccaaacg tacgccggtg cggcagtttc gcggcattgt ggctccgggc agtggtggca
    22981 gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag
    23041 atgaagccgg aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta ttcgagtcat
    23101 attctgaaat atccagcgga tcaagaaagt tcgttggata tttttttgca tggataaaat
    23161 tatcgcctct aaagtatgta ataacaggga atgtgatgaa aaaagtttta ttgcaaaacc
    23221 atcctgggag cgagaagtat tcttttaatg gctggggaat atttaatagt aattttgaac
    23281 gggtgattaa agaaaataag gccatgctgc tttgtaagtg ggggttttat ttaacatgtg
    23341 ttgtcgctgt aatgtttgta ttcgcagcga taacatccaa cggtttgaat gaaagaggcc
    23401 tgattaccgc gggatgctct tttctttatc tattaattat gatggggctt attgttcggg
    23461 ccggttttaa agcaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc
    23521 tcagtatcga gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc
    23581 aatggtctga aacgctagag ttctggccgc gaaagcctga acctggcaaa gataccttcc
    23641 aatatcatgt gcttcctttt gactcgatcg atatcatcag taaaagacgc gagtctttag
    23701 agaatcaatg gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt
    23761 ctggcgacca tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta
    23821 tcgccttgtt gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata
    23881 atcgctccgg taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct
    23941 cgatatcaag cacagctttt caaaacggga caattgaaga agaactggcc tggcattata
    24001 taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa
    24061 atagtctaat gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg
    24121 ctgaacttga agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat
    24181 gccgttggcc cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc
    24241 ctgaaatttt gccgggggcg ctgcgcttga ccggcctaca ttagagcgta actgcaatga
    24301 attgtgccca tctgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca
    24361 caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca
    24421 cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca
    24481 tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt
    24541 gtgccatttc caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa
    24601 acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatatgccc
    24661 cttcagcgac ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt
    24721 cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt
    24781 cgagatagac cagaccaaat tctttggcgt aatctgccgc tcgaccatcg cggctggctt
    24841 tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc
    24901 cgctggagtc tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt
    24961 actgggcgac gttgccggag ccagaaacgg aaacgctcat cccttcaaaa cccataccgt
    25021 ggcgttttaa cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa
    25081 taagactacc gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga
    25141 gctttttcat catccccgcc ataaagccga cttcacgacc accaacgccg atatcacctg
    25201 ccggaacgtc ggtatccgcg cccaggtggc gatacagttc agtcatcagc gtctggcaaa
    25261 aacgcatcac tttaccttcg ctttttcctt t
//
LOCUS       DAJTRM010000058        24844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRM010000058 DAJTRM010000000
VERSION     DAJTRM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.058"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1039
                     /gene="proB"
                     /locus_tag="OO322_002460"
     CDS             <1..1039
                     /gene="proB"
                     /locus_tag="OO322_002460"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5907299.1"
                     /translation="LNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELP
                     ATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRAL
                     LDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRRN
                     PQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
                     VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPK
                     GIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGP
                     VAVHRDDMITR"
     gene            1051..2304
                     /gene="proA"
                     /locus_tag="OO322_002461"
     CDS             1051..2304
                     /gene="proA"
                     /locus_tag="OO322_002461"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5907300.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2419..2494
                     /locus_tag="OO322_002462"
     tRNA            2419..2494
                     /locus_tag="OO322_002462"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2452..2454,aa:Thr,seq:cgt)
     gene            complement(2509..3669)
                     /locus_tag="OO322_002463"
     CDS             complement(2509..3669)
                     /locus_tag="OO322_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5907301.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPA
                     YRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALK
                     EMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3561..3893)
                     /locus_tag="OO322_002464"
                     /pseudo
     CDS             complement(3561..3893)
                     /locus_tag="OO322_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3980..4324)
                     /locus_tag="OO322_002465"
     CDS             complement(3980..4324)
                     /locus_tag="OO322_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907302.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4425..4604)
                     /locus_tag="OO322_002466"
     CDS             complement(4425..4604)
                     /locus_tag="OO322_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HCR5907303.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4701..5630)
                     /locus_tag="OO322_002467"
     CDS             complement(4701..5630)
                     /locus_tag="OO322_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5907304.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5633..5824)
                     /locus_tag="OO322_002468"
     CDS             complement(5633..5824)
                     /locus_tag="OO322_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907305.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(5826..6455)
                     /locus_tag="OO322_002469"
     CDS             complement(5826..6455)
                     /locus_tag="OO322_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5907306.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6452..7027)
                     /locus_tag="OO322_002470"
     CDS             complement(6452..7027)
                     /locus_tag="OO322_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HCR5907307.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7024..7188)
                     /locus_tag="OO322_002471"
     CDS             complement(7024..7188)
                     /locus_tag="OO322_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR5907308.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7199..7492)
                     /locus_tag="OO322_002472"
     CDS             complement(7199..7492)
                     /locus_tag="OO322_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR5907309.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7516..7899)
                     /locus_tag="OO322_002473"
     CDS             complement(7516..7899)
                     /locus_tag="OO322_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907310.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(7899..8504)
                     /locus_tag="OO322_002474"
     CDS             complement(7899..8504)
                     /locus_tag="OO322_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HCR5907311.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8606..8764)
                     /locus_tag="OO322_002475"
     CDS             complement(8606..8764)
                     /locus_tag="OO322_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907312.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTETHVH
                     AESSLVSP"
     gene            complement(8761..8913)
                     /gene="kil"
                     /locus_tag="OO322_002476"
     CDS             complement(8761..8913)
                     /gene="kil"
                     /locus_tag="OO322_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR5907313.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(8898..9032)
                     /locus_tag="OO322_002477"
     CDS             complement(8898..9032)
                     /locus_tag="OO322_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907314.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQPEALCAMK
                     "
     gene            complement(9227..9697)
                     /locus_tag="OO322_002478"
     CDS             complement(9227..9697)
                     /locus_tag="OO322_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907315.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9706..10032)
                     /locus_tag="OO322_002479"
     CDS             complement(9706..10032)
                     /locus_tag="OO322_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCR5907316.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(10396..10800)
                     /locus_tag="OO322_002480"
                     /pseudo
     CDS             complement(10396..10800)
                     /locus_tag="OO322_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10797..11429)
                     /locus_tag="OO322_002481"
     CDS             complement(10797..11429)
                     /locus_tag="OO322_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5907317.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            11533..11748
                     /locus_tag="OO322_002482"
     CDS             11533..11748
                     /locus_tag="OO322_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5907318.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            11865..12146
                     /locus_tag="OO322_002483"
     CDS             11865..12146
                     /locus_tag="OO322_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907319.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            12181..12342
                     /locus_tag="OO322_002484"
     CDS             12181..12342
                     /locus_tag="OO322_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907320.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            12329..13159
                     /locus_tag="OO322_002485"
     CDS             12329..13159
                     /locus_tag="OO322_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5907321.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            13156..14532
                     /locus_tag="OO322_002486"
     CDS             13156..14532
                     /locus_tag="OO322_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5907322.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            14605..14931
                     /locus_tag="OO322_002487"
     CDS             14605..14931
                     /locus_tag="OO322_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907323.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            14928..15128
                     /locus_tag="OO322_002488"
     CDS             14928..15128
                     /locus_tag="OO322_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907324.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            15140..15328
                     /locus_tag="OO322_002489"
     CDS             15140..15328
                     /locus_tag="OO322_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907325.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            15331..15495
                     /locus_tag="OO322_002490"
     CDS             15331..15495
                     /locus_tag="OO322_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907326.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            15476..15916
                     /locus_tag="OO322_002491"
     CDS             15476..15916
                     /locus_tag="OO322_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5907327.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            15913..16503
                     /locus_tag="OO322_002492"
     CDS             15913..16503
                     /locus_tag="OO322_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine-binding protein"
                     /protein_id="HCR5907328.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            16500..16673
                     /locus_tag="OO322_002493"
     CDS             16500..16673
                     /locus_tag="OO322_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HCR5907329.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            16640..16815
                     /locus_tag="OO322_002494"
                     /pseudo
     CDS             16640..16815
                     /locus_tag="OO322_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            16818..17219
                     /locus_tag="OO322_002495"
     CDS             16818..17219
                     /locus_tag="OO322_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907330.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            17179..17409
                     /locus_tag="OO322_002496"
     CDS             17179..17409
                     /locus_tag="OO322_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR5907331.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            17381..17992
                     /locus_tag="OO322_002497"
     CDS             17381..17992
                     /locus_tag="OO322_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCR5907332.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            17989..18195
                     /locus_tag="OO322_002498"
     CDS             17989..18195
                     /locus_tag="OO322_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HCR5907333.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            18173..18838
                     /locus_tag="OO322_002499"
     CDS             18173..18838
                     /locus_tag="OO322_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR5907334.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            18835..19458
                     /locus_tag="OO322_002500"
     CDS             18835..19458
                     /locus_tag="OO322_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5907335.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            19891..20214
                     /locus_tag="OO322_002501"
     CDS             19891..20214
                     /locus_tag="OO322_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5907336.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            20198..20674
                     /locus_tag="OO322_002502"
     CDS             20198..20674
                     /locus_tag="OO322_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5907337.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20671..21108
                     /locus_tag="OO322_002503"
     CDS             20671..21108
                     /locus_tag="OO322_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5907338.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            21313..21855
                     /locus_tag="OO322_002504"
     CDS             21313..21855
                     /locus_tag="OO322_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5907339.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSYTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            22075..22290
                     /locus_tag="OO322_002505"
     CDS             22075..22290
                     /locus_tag="OO322_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5907340.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            22438..22680
                     /locus_tag="OO322_002506"
     CDS             22438..22680
                     /locus_tag="OO322_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR5907341.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            22716..23204
                     /locus_tag="OO322_002507"
     CDS             22716..23204
                     /locus_tag="OO322_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5907342.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            23182..24681
                     /locus_tag="OO322_002508"
     CDS             23182..24681
                     /locus_tag="OO322_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5907343.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            24682..>24844
                     /locus_tag="OO322_002509"
     CDS             24682..>24844
                     /locus_tag="OO322_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907344.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRG"
BASE COUNT         6643 a         5851 c         6087 g         6263 t
ORIGIN      
        1 tctgaaccgt gcccatatcg ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg
       61 gcatcggatt gttattgtga cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta
      121 cccggaactg ccagcgacta tcgcctcgaa acaactgctg gcggcggtag ggcagagtcg
      181 actgattcaa ctgtgggaac agctgttttc gatttatggc attcacgtcg ggcaaatgct
      241 gctgacccgt gctgatatgg aagaccgtga acgcttcctg aacgcccgcg acaccctgcg
      301 tgcgttgctc gataacaata tcgttccggt aatcaatgag aacgatgctg tcgctacggc
      361 agagattaag gtcggcgata acgataacct ttctgcgctg gcggcgattc tggcgggtgc
      421 cgataaactg ttgctgctga ccgatcaaaa aggtttgtat accgctgatc cgcgcagaaa
      481 tccgcaggca gaactgatta aagatgttta cggcattgat gacgcactgc gcgcgattgc
      541 cggtgacagc gtttcaggcc tcggaactgg cggcatgagt actaaattgc aggccgctga
      601 cgtggcttgc cgtgcgggta tcgacaccat tattgccgcg ggcagcaagc cgggcgttat
      661 tggtgatgtg atggaaggca tttccgtcgg tacgctgttc catgcccagg cgactccgct
      721 tgaaaaccgt aaacgctgga ttttcggtgc gccgccggcg ggtgaaatca cggtagatga
      781 aggggcaact gccgccattc tggaacgcgg cagctccctg ttgccgaaag gcattaaaag
      841 cgtgactggc aatttctcgc gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga
      901 tatcgcccac ggtgtcagtc gttacaacag cgatgcatta cgccgtattg ccggacacca
      961 ctcgcaagaa attgatgcaa tactgggata tgaatacggc ccggttgccg ttcaccgtga
     1021 tgacatgatt acccgttaag gagctggccg atgctggaac aaatgggcat tgccgcgaag
     1081 caagcctcgt ataaattagc gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa
     1141 atcgccgatg aactggaagc acaaagcgaa atcatcctca acgctaacgc ccaggatgtt
     1201 gctgacgcgc gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc
     1261 gcacggctga aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg
     1321 gggcaggtaa tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta
     1381 ccgctggggg ttattggcgt gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct
     1441 tcgctgtgcc tgaaaaccgg taatgcggtg atcctgcgtg gtggtaaaga aacctgtcgc
     1501 actaacgcgg caacggtggc ggtgattcag gacgccctga aatcctgtgg cttaccggcg
     1561 ggtgccgtgc aggcgattga taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg
     1621 gataaataca tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt
     1681 gaacagtcga caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat
     1741 gaaagtgcag agatcgctga agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg
     1801 agcacatgta atacggttga aacgttgctg gtgaataaaa acatcgccga tagcttcctg
     1861 cccgcattaa gcaaacaaat ggcggaaagc ggcgtgacat tacacgcaga tgcagctgcg
     1921 ctgacacagt tgcaggcagg ccctgcgaag gtggtggcgg ttaaagccga agagtatgac
     1981 gatgagtttc tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc
     2041 gcccatattc gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc
     2101 aacgcccagc gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg
     2161 cgttttaccg acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa
     2221 ctccacgcgc gtggcccaat ggggctggaa gcactgacca cttacaagtg gatcggcatt
     2281 ggtgattaca ccattcgtgc gtaaataaaa ccgggtgatg caaaagtagc catttgattc
     2341 acaaggccat tgacgcatcg cccggttagt tttaaccttg tccaccgtga ttcacgttcg
     2401 tgaacatgtc ctttcagggc cgatatagct cagttggtag agcagcgcat tcgtaatgcg
     2461 aaggtcgtag gttcgactcc tattatcggc accatttaaa tcaataagtt acctcgcatt
     2521 taagtaaacc acgttctcct cttgtgccgt atttgtgcca ttgcgactta taatcgcatc
     2581 gattttgctt gcgtgctcgg tgagatgccc ggctgaaagg tgggcgtatc tttgaaccat
     2641 ttcgagagtt tcccatcctc ccatctcttt aagtgcaaga agagagacac cggactgaac
     2701 cagccagctt gcccaggtat gcctcagatc atggaagcgg aagttgctaa tgcctgcccg
     2761 ctttaacgct cccttccatg ccttgttgct gtcggttctc atcttcctta ccgctgctgt
     2821 ttttgttccg tcgcttcggt aggcaggttt ggtgtggaca aatacccatc tcttatggag
     2881 cccctgctgt tttcttaata tctggcatgc ggtttcgtta agaggaactc cgatcgcatt
     2941 gccagctttt gtttcatcag ggtgcatcca tgccattttc ttatccagat cgacctgtga
     3001 ccactcaagg tctgtaacgt tggaacggcg aaggcctgtc gtgattgcaa acatgaccac
     3061 agggaagaag tgaggagcaa tttctgcaaa caggcgcttc gattcctcct ctgtaagcca
     3121 tctgatgcgt ccattcttaa cgcgtggtgt tgatattttg ggtgccctgt caagccatcc
     3181 ccattcaaca gccatattga gaatggcgcg aagtattgcc agatgcctcg tcttcgttcc
     3241 tttgcttgca agctttggtt tatactccgg caccggattg ccaagtcgca aacacctgtc
     3301 ccggctcatc tcccagttca ggcgatggcg gcggttttcc atcccgtcta ccgcctccat
     3361 tattttttct gttgttatgt cagagagaat ggcttctctg aagtgcaaca tccagaacga
     3421 tataatgctc ttgtcatcat caatggactt cttatccgat ttctcacgca gccaccgtat
     3481 gcaggcttcc ttgaatagct ttttcggtga ttccccgaga ttttttactc tccacgcttc
     3541 tgctttcaga cgatcgtgaa gttcttgcgc ttgccttttg tccgatgttt caagagagcg
     3601 tctaactctt gatccatctg gcgcgacgaa atcgcagtgc cacgtgccac cgcgtagttt
     3661 gattgacatg ctttaacctc ctgcacatca accgcattca ccgcgctatt gtgtctcaca
     3721 gacttaagcg ccgcaatgca gtctgacttg caaatgcgat atgggctttt aggtttatct
     3781 ggatttatct ttgcggcctg aagtcgtcca cttcgtatcc actgcgtgat agtgcctttg
     3841 tctaccttca gatacgacgc tgcctcttca cgagtgaaga tttcttcttc cacctggaat
     3901 ctccatttat tggattggta ttattgcggt aggtctggat atcattgagc aatgaacagg
     3961 cctcatcgag tgtgaggcgt tagtccttgc gtagctcgct gattcttctg taagtctctg
     4021 gtgctttgtt cccgtacgtc ttcatttcag acttcaacag agcaacgagt gaatcccatt
     4081 cgttgaggat tcctttgaat gccggaacgc gctttgcaac cttgtcgaat gaatctctga
     4141 tttctggaat ctgctcaaca agtgcaacgc atcgccggaa atcggctgcg tcatgtggag
     4201 caccgaagct atgaccatag atattctttt tcagtccaca tgcgattgag gcaagagttg
     4261 cgctactgat gccgacatcg tcagtcgatt gccatttcaa aaccttcata gccaaatctg
     4321 acatttcttg tctccaataa aaaaccgcca tcaggcggct tggtgttctt tcaattcttc
     4381 aattcgaata ttggttacat tgttttcata tatgaataaa taaattagct tttttcgttg
     4441 ccttcgcgtt ctttattaat tttgacaaac tcgtttttac cacgctctcc aaatgcgtct
     4501 ttagagtcgt tgtatccgca atcgcagcac acataatcac cagaccatcc acgcattgtt
     4561 ttttcttttg caatatttcc agaaccgcat tttggacaag acatatcact acctccaaag
     4621 catgagtgag atgacaacgt aacattgatt ggagattaac aatagattgc tgatgtaaaa
     4681 gatatgtata agcttcgctt tcaaagtgga ggctctggta gcggcatcca gtgggttaca
     4741 ttgctaatca gaccatattc attagtttga ggatggttgc cgttatcgtc tccgtattta
     4801 agactctcca taaaaccata atgcttattc ccattaacgc tcacaaagcc gtaataagct
     4861 ggtataacgc cgatctcaca cgtaatcagc aaaggaaaac tagttctcca atttagctcg
     4921 ccgattacag gcattctctc actacagctt atccaaccat ccggagttac cggaacttgc
     4981 ggaatggctg tctgctctcg aacgtcatta ggcgcgatag gttctgctgc caactgactg
     5041 gcatatttgt taatggtaac gataagctct tgctcagcct catccagaca atcaccgata
     5101 cctcgcctgt caccgtcaaa atcatcgaaa tcggcacgaa tcttggcaac cttctggatt
     5161 gcggacaaca cctcactagg aattaccgga tagttggttg acgtttccgc gatttcacga
     5221 aaattattgg ttgacgaatt cttgttttcc cgaaagtttc cggactgaag catggctgcg
     5281 cgataggcgt tccagccgac agcttttccg tgttcaaacg cgctgtcaaa gtcatcatcc
     5341 atttccatcg catcaggcac agataccgtc tctggagggg cggcgtaaag cggagttacg
     5401 gtaatttcgt actcgtttat atcctcgtcg ctgatatttg cgaaatagcg aaggatgcct
     5461 ttctctccac gactacgagc gtcagagtcc tgaatgatat acgccacagg ctctgcttcc
     5521 agcgatgcca gcgcaattcg tgccagttcc atttgttcgc cacgagtaag cccgttttca
     5581 agcggatttt taatgaacaa ttcaatacgt tctttggtaa tagtggtcat agctatttca
     5641 ccttaatctc aatatttcgc agctttagct ctactggcag gtctgacttt cctgttaacg
     5701 ctaatgcgag attttctgga gtaatgagag cagttattgt tttccccatc gccagacgaa
     5761 taatcagtcg tatctcgcga tcgtcacatg ctcctggtcg aacaattgat atttgtccgt
     5821 ccatctcact ctcctttgat gcgaatgcca gcggcgcgga ttgcagcgat gacctcagaa
     5881 actttgtatg ccattaccgt ttggtaatca tcgtgaaaat ctgttcgatg aagcatgctg
     5941 ctacgttccg ggagcagtat tccccgctcc cccagttctg ctatgcgatt ctctgcggat
     6001 tccagcgccg caaccaattc gtctacagtt ccggcagctt gcagtgcgta atcggtaata
     6061 gccatctcat gatcaatttc agtaccgttc tcattcgttg aggtgatagc aaaataatca
     6121 gagtcgattt cgttatcagc taagtggcgt agcgtatcgg caacaagccg gccgttttcg
     6181 attagcagct tccctaccgt tagcgcaata tcctcgttct cctggtcgcg ggatttgatg
     6241 tattgctgat tccttacccg ttcatccagt agtgccagca cggtttctgg tccggccaga
     6301 aatttgaagg cgttgagcgc atcaatatcc acaccgtaat ctttaagttc ctgttcactt
     6361 aacaaatcat cagctggcaa cattaacagg cgttccattg ctggaattgc acgttccgcc
     6421 gcctcacgca gtgcctgata gttaatttcg ctcactggcc gcctcctttg cgaagctggg
     6481 cggcgaactc gttagccatc gctgaatatg ggtgctcatg accgccaaca accttcatgc
     6541 tggcagcaaa catctccacg ccctgcgccc gcacttcatc caggaaagtg tcggtggctg
     6601 gggtttctgg aatggcgtct ggaataacct ctgagtagac gcgatccatt gccgactccc
     6661 atccgtagta acaagccgaa tagccgtcgc gctggtagcc gtggtcttca actccacatc
     6721 ccatgccctg gtcatggtat tcaggctgat tgtcgggatt aatgcagtat tcaatgaccg
     6781 acttcatccc cgcattctcc gccgccagcg ccgaaaactt ctcgtgtgcc aacttaacag
     6841 ccgcatcagc ctgcttaatt gactcaatcg ctttctggtg gtcttcgtac agagccgaaa
     6901 tcttggcctc cgcttcagca aatttacgca ccagatattc agcgtttgtt tcgttaacct
     6961 ttaaatcaca tgggatgcat ttacctttca gaaaaccatc catctcaatt agtgacattt
     7021 gtttcatttc ttcccactcc gccacatagc attcagatat ttgttgtcat taacagaacc
     7081 gaaactcttt ctcttaagta attcctctct cgatggcatt ggctttacgc gttggcgaat
     7141 aatcatttct gccggaagaa tgccgggatt gtatgcaagt cctctcatgg taaattcctc
     7201 agtcattact gatagcgcca tagcgtgagc ggtaattacg caggcgcggg tcaatttcag
     7261 ggaagtgggt atatgtggct ttgcggaatg gtcggattga tgtctggtaa attcgctcga
     7321 gttcttcttt ctctgcaagc catatacagt ggcgaaattc cttttcctct ttcgtttcct
     7381 gcggtagcga cattatccga tcgtagtttt ttctgaattt atccagcacc tccgatacgg
     7441 aattgccgga acagcggcgt gggtcatccg caccatacag aggcgctggc ataatggaat
     7501 ccttatgttg ctactttaga agggaattga atcgtcgtat tcaggatgat tttgatgatt
     7561 gctactttgc tgctgttggc tgtttcctga agttgcaaat ccaatctttg cattcagtaa
     7621 ttcaagagtg attgattgtc cattttgccc ctgataaaca tcaaccctga tgttttctcc
     7681 ggtaatttcc acaatgccac cttcaacaag aacactacgg tagtaatccg cttgcgctcc
     7741 cggcttggca aatacaacgg cgctgtagtt tgtccattct ttcttttttg tctggcgatc
     7801 gtaatactga acgccagcac ggatgttgaa tccgatattt tccccggcct gaaactctct
     7861 tgcgggcttg tttagtctta cagtaatcga atgtgccatt aagcagccgc tccttctaat
     7921 tcgtctcgtc tgatgttgta aatgtcctgc gctttgtgct gctccggtgt gccttcgagc
     7981 atcttccacg ctttggcgaa cgcctgttta agctcttcca cggtgttttt ctgcaatgct
     8041 gcgtcagtga atgcttttag aacctgttca ggtgtaggtg atggttttga ttgctttgct
     8101 gctgcgttct gctgatgttt atgctcgtcg gtatctgcat ctttcgcatc atcaatgccg
     8161 aacaaaccat tgaggcaata cttgcgtgca taagagcttg tggctcccgt aacttgtgca
     8221 gaatccattc ctttcttgct ttcttcctct cgtgcaagag cggttgccgt atgactgttt
     8281 tcgccatcgg taatagttgc cgtggctttc acgtaatacc gatcaccaat caacacaact
     8341 tcatcgctga ttgataaaaa cagaccattc agtaacggct taacgccttc aagaatgtct
     8401 tcgcagcttc tgtatttata tttaccgaat gagttgtact ggttctttgg cgcgttcaag
     8461 ttctcctgaa tagctgccag tcttgcgtaa aattctttgc tcatatgttt gttctcagaa
     8521 tgggcatggc ccaaggaaat aacgctgatt taatacttcg actcgggaca aattaaggca
     8581 tacccgcatt ccttcgcggt cgccattatg gcgataccag agagctttct gcgtgtacat
     8641 gcgtctctgt aacttgctct ccttcactgt ggttgcaagt gacatgaata tctccttcgt
     8701 taccgattaa ttctttcatc tgacgaatga attcttcgtc tgaccagtta tctgtaaaac
     8761 tcatttcctg cgataccacg gaatattgat agctgatttc atcgctttat ttgcttcaag
     8821 ccacattttt gaatcaccaa taaatctggc tattactgct ttgttttgtg ctgcacgaag
     8881 catctggtga ttgatggcta tttcattgcg cataacgcct ccggttgttt ctttgctgca
     8941 ctgattaatt gtttaactcg gcgtgataat tcagattcgt gcgggtagaa agcggacatg
     9001 acgccgctac ccgcgagctg aaagtgcatc atggataact ccttctgttt gattgcataa
     9061 cgaaaacgcc tcgagtgaag cgttattggt atgcatataa aaaagccctc atactggagg
     9121 gcaaagaaga tttcaaataa tcagaacaag tcggctcctg tttagttacg agcgacattg
     9181 ctccgtgtat tcactcgttg gaatgaatac acagtgctta ctcgtactaa taaaataccc
     9241 aattttctgt ttcttggttg tgcccaaagt tatattcaat atctggtgtt gatgtatcaa
     9301 tattcttcat cccatcaaca agagttgata caacagccaa atcctgtttg attctcatta
     9361 aatggtattt cttccggcgc aataaacttt caatggcaag tttcttcgtt gggaatgcaa
     9421 aagatctttc tgcatttttt gctactttct taattgcata tctatttctc ttttgtttcc
     9481 attcctgtaa ccactgattt ggtgctggtt taaaattaac aatccaatgc gcaggaacca
     9541 accatgcata atgctctgtc tgatgaaaag ctatatattg aagtgcgaat attttgattc
     9601 catcttcttc aactgtcgcc tggaatctcc agaaaacagg cattccatca tgttcagttt
     9661 ctgattcagg aaaaggtacg ctccatgatt ttgtcatatc tcacctcaaa taagtggctt
     9721 gctgcctaat ttcattttct ggcgaccaac acaagtcaca cccatttcac tgcgtggctt
     9781 gctgtaccat gtgcgctgat tcttgcgctc aatacgttgc aggttgcttt caatctgttc
     9841 gtggtattca gccagcaccg taaggtctat cggattcagt gcgctttcta ctcgtgattt
     9901 cggtttgcga ttcagcgaga gaatagggcg gttaactggt tttgcgctta ccccaaccaa
     9961 caggggattt gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt
    10021 ttgtgcatcc atcttgattc tcctgtcagt tagctttggt ggtgtggtag gtgggagacc
    10081 catttcgacc tgcttcggcc gacttcaatt cggcaatagt tccgcaggcc tcgccgcttt
    10141 acgtgcgaca tattcccatc catgaaccct tcaccacacc ccaaagcctt ctgctttgaa
    10201 tgctgccctt cttcagggct taatttttaa gagcctcacc ttcatggtgg tcagtgcgtc
    10261 ctgctgatgg cttaaaatta caagaaagat tgtatgttgt aaacaagaaa tattgtaaaa
    10321 aggggcgtaa aaacaaactc cattgttttt aaatgaaaaa tagtttgttt ttggttatcg
    10381 agattgaggt ggggattact ggttgcaggt tccgactaca tcaccaacaa atgatttggt
    10441 tgatgtaagt tgttgcatac ctgggatatt cattactttg gagtaaagag cttttttgtc
    10501 tgtagtgatt gatcaagttt caacggttat tcctcctcca gactggtatt ctcctaccat
    10561 agtgttcgat gacaaagcag tgtatttcat ctctggatag acgccagaaa ctgattcata
    10621 aactgatgat ttatcgccat ttattgttac gtggaaaacg gaatcttccg tgctgtcttt
    10681 tgtaaactcg taacgatcgc cattcattga cccgtacccg tgcaggtttg tgacaatcca
    10741 gcattcagaa ttggcgctgg tagttaagag tattgagagt agcgccgcaa tcctgatcat
    10801 acgaatttta ccctcgcttc cacgacaaca ccgataatct tgcagttccc gttgatagga
    10861 gtcataggcc atgaagggtt caggcctttc aggtacttct gcccgccatc tataaccagt
    10921 ttcttgaatg ttgcttcgtt cgcgtcagtc agtttggcta caacaaggct tccattcact
    10981 ggctcgcgtc cagtatctac taacaccata tgaccttcag ggatgctttg acctacaggt
    11041 gaggtcatgg aatcaccttc aaccttcagc cagaatccat cgcctaataa gttaacgtca
    11101 ctgtcatacc attcatcaat gtccttgata tcgtagggtt cacaagcttc acaccacgaa
    11161 ccagctctaa ccatgctaat caatggatat ttccctttgg gctcaacatg cccaacaaat
    11221 ctaacattcg aatcagaggt gccattgagc agccagtcaa cacttacgcc aagagctgac
    11281 gcaagttctg gtaaaaagcg tggtcgctta gttttaccgt tttcgagctg ctctatagac
    11341 tgctgggtag tccccacctt ttgagcaagt tcagcctggt taagtccaag ctgaattctt
    11401 ttgcttttta ccctggaaga aatactcata agccacctct gttatttacc tccaatcttc
    11461 acaagaaaaa ctgtatttga caaacaagat acattgtatg aaaatacaag aaagtttgtt
    11521 gatggaggcg atatgcaaac tctttctgaa cgcctcaaga agaggcgaat tgcgttaaaa
    11581 atgacgcaaa ccgaactggc aaccaaagcc ggtgttaaac agcaatcaat tcaactgatt
    11641 gaagctggag taaccaagcg accgcgcttc ttgtttgaga ttgctatggc gcttaactgt
    11701 gatccggttt ggttacagta cggaactaaa cgcggtaaag ccgcttaaga cattcccgct
    11761 cttacacatc ccagccctga aaaagggcat taccagaaac aaatctctat ggttttgcgt
    11821 ttctttgcga agccaactct atctaatcat taaggaaatt atctatgggt actattgcaa
    11881 ctaaaagcaa gaaagcggct cgcatcgagt cagccttgct gaacaaactg gcactgatgg
    11941 ggcagaagac attcgctcga gcaatggggg ttcctgaata tcaggtaagc cgatggaaga
    12001 atggtttctt ctcgcaggta agcatgatgc ttgctgttct ggaatacgga atcgaagacg
    12061 atgaaatggc tgaattgact aagcggcttg ccgattacct gacaaaagaa aaagccccga
    12121 agaacggcga attcttcgag gcctaatgta gaaagactgg atcaatccac gggagtaatt
    12181 atgacaaaac gtcgtaagaa ataccaggaa aaagaagaga ttcgacaccc tgattcacct
    12241 gagggattag tggtagccgc agcaaataac agggcgttcg cagagcgcct tgttggtgtt
    12301 tacagactag ccaaagcagg agtgaaacat gggcgtcgtt aagttagctg attacaggcc
    12361 tcaactggag gtcgtggagc atcgcgtggc agaactcgaa gatggctaca ctcggactgc
    12421 aaacacactg ttagaagccg ccatgctttc tggacttact ctacatcagt tactgattgt
    12481 tatggctgtg tggcgcaaga catacggtta caacaaaaaa atagattgga tcggaaacga
    12541 acagttcgct gaactcactg gcatggcacc aaccaaatgc tctaccgcca aaaacgagct
    12601 tatcagaatg ggagttctca ttcaggtggg gcgtcaagtt ggtatgaata caaacatttc
    12661 cgagtggaaa acgaaagtta acggattcgg taaaacattt accaattcgg taaaacaaac
    12721 cttcaccaaa tcggtaaaaa gcaatttacc gaatcagtca aacacaaaag acaatataca
    12781 aaagacaata aatacaaata cccccttacc ccctaacggg ggcggcgatg ggcaggttaa
    12841 acctgaacgt cgcaaggcag aacgaatcga ctacgaatcc ttcctgaacg cctacaacac
    12901 cgaagtcggt gacagactgc cacacgctgt tgcggtcaac gagaaacgca aacgccgcct
    12961 gaagaaaatc atcccgcaac tgaaaacgcc aaacgtggac ggtttcagag cgtatgtcag
    13021 ggcgtttgtg catcaggcca agccgtttta cttcggagac aacgacacgg gctggacggc
    13081 cgattttgat tacctgctga gggaagactc gttaacgggg gtacgggaag ggaagtttgc
    13141 agacaggggg attgcatgaa acaggatatc gaagcgagcg ttatcggtgg cctgctgatt
    13201 ggtggattaa caccaaccgc cagcgacgtt ctggcaacgc tggagccgga agcgttttca
    13261 attccgctct accggaaagc cttcgaggtt atccgcaagc aggcgcgaaa cagaaaccta
    13321 atcgacgcgc tgatggttgc cgaggcgtgc ggagaggagc atttcacgtc aatcctgatg
    13381 accagcaaga actgcccgag tgccgcaaac ctgaagggat atgctggaat ggtcgcggat
    13441 aactatcacc gccgtctggt gctggaaatc atggatgaaa tgcgtgaacc aatccaaagc
    13501 ggaaccatcg acgcatcgag tcaggcgatg gatgaacttg taaagcgtct ttcagccatc
    13561 agaaagcccc gtgacgaggt aaaacctgta cggttagggg aaatcatcac tgactacact
    13621 gacacgcttg acaggcgtct gaggaacgga gaagagtcag ataccctgaa gaccggaatc
    13681 gacgaacttg acgccatcac cggagggatg aacgcagaag acctggtgat tatcgccgct
    13741 cgtcctggta tgggtaaaac cgaactggcg ctgaagattg ccgaaggcgt tgcaagccgc
    13801 gttattcctg gttctgacgt ccggcgcgga gtgttgattt tctcgatgga aatgagcgca
    13861 ttgcagattg cagagcggag cattgccaac gccgggagga tgtcggttag cgtgctgcga
    13921 aatcctgcat cgatggatga cgaaggctgg gcgcgtgttg ctaacggcat gagtcagctt
    13981 gcagatttgg atgtatgggt agtcgatgcc tcgcggttat cggtcgaaga aatacgctca
    14041 atcgcagaac ggcacaaaca ggaaaatcca aacctgtcac tcatcatggc ggattatctt
    14101 ggcctgattg agaagccgaa agcagaccgc aacgacctcg caattgctca catctccgga
    14161 agcctgaagg cgatggcgaa agacctgaaa acgccagtta tctccctgag tcagctttcg
    14221 cgcgatgttg agaagcgacc aaacaaacgc ccgacaaacg cagatttgcg tgattcagga
    14281 agcattgaac aggacgcaga ctcaatcatc atgctctatc gggaagcggt atatgacgag
    14341 aacagtagcg ccgcgccatt tgctgaaatc atcgtgacga aaaaccgttt tggctcgctt
    14401 ggtacggttt accagcggtt ctgtaacgga cactttgttg catgtgacca ggatgaagcc
    14461 agacagattt gcacagcatc aaatgcacct gctgcgcgtg gcagacgata tgcacaaggg
    14521 gctgacgtat gaccatctac atcactgaga taataacagg cctgctggta atcgcaggcc
    14581 tttttatttg ggggagaggg aagtgtggct gactggcaaa ttccaatcat cattcttgcc
    14641 ggagcttcgc tggttgctgg ctttatcctg ctgaaaaagc ataaagaccg tgatcaaaaa
    14701 gttgaagttc tctatgggta tccagcgaac agcacaacat ggctgaccat ttaccactac
    14761 cgaaaatcag gacgctgggt attcgaatgg gatgatctgt tcgctgaaaa gcgaccaaag
    14821 tcatggggag acatcagcga atgcatgatg tttgaagaaa gaaaatccgg cgcaacccga
    14881 gaagagttta acgaagcgtg ggcgcgatta agtgagagag ggtatttgtg agcaagtacg
    14941 aaaaattaga tcaaaacatt ctttcaatgc tgagtgaaag accaacacct gtttttgata
    15001 tctggcttaa atggcggagc aatggaatgt atatcgaaac catcgatcgc cgtatgcaat
    15061 acctgagaaa gaaagggctt gttgcaaatg tgcgtgggaa gggttgggtg aaaattaacc
    15121 tgtcataacg gggattgata tggacgatag cttagaaata tttgaagagt ggtgcgctga
    15181 tgaacttggt gtctccgctt acttcattcg acaaatgcga agcaagaata ttttgggcgt
    15241 gattgagtat aaacgagttg aaataaacaa gagatacagg gcttggatgg ccgcagttcg
    15301 cgccgccgga gtcaaagtga aggagtgagt atgagcgcat acgaagaaat catgttagcc
    15361 ctgcgattct ttttcggtgt ggaagaagat gaaaacgtaa atgagattat cggacaagac
    15421 catgacccga tagggactat tgcagctgca cttgacgatt acaggagcgt aaatggtgag
    15481 gaaactaacg tttgaactaa gaagccccat ccatcagcag aacgccattc aagctatcca
    15541 gcaaatcctt ccagacccaa ccaaaccaat cgtagtaacc attcaggaac gcaaccgcag
    15601 cttagaccag aatcgaaagc tttgggcttg ccttggtgac gtttcgcgtc aggttgaatg
    15661 gcatggtcgc tggctggatg cagaaagctg gaagtgtgtg tttaccgcag cattaaagca
    15721 gcaggacgtt gttcctaacc ttgccgggaa tggatttgtg gtaataggcc ggtcaaccag
    15781 caggatgcgt gtaggcgaat ttgcggagct attagagctt atacaggcat tcggtacaga
    15841 acgtggcgtt aaatggtcag acgaagcgcg actggctctg gagtggaaag cgagatgggg
    15901 agataaaact gaatgaacaa ataccgactt atttacgcag atccgccttg gcaatatcgc
    15961 gacaaagcca acgatggcaa tcgcggtgct ggacataaat acgatgttat gaatgttcag
    16021 gacatttgcc gactgccagt atgggattta gcggatccag aatcttgctt gttagcgatg
    16081 tggtgggtgc cgacacagcc agccgaagcg ctaaaggtaa ttgaggcgtg gggattcagg
    16141 ttgatgacta tgaaaggctt tacttggcac aaaaccaata agcacaaagg caacagtgcg
    16201 atcggaatgg ggcatatgac cagggcaaat agcgaggatt gcttgtttgc cgttcgaggg
    16261 cggttacctg agagaatgga cgcttcaata tgccagcact ttaccgcacc gagaatggag
    16321 cacagtgcaa aaccaccgat cgtaagagac atgttagcta agttgcttgg cgacgtgccg
    16381 cgctgtgagt tatttagccg cgacaaagtg accgggtggg atatgtgggg aaatcagtgc
    16441 gactccgatt ttgaacttgc tcccggcatg gcgattaaac catgcaaaat ggtgatcgca
    16501 tgaagcactg ttatcgatgt ggagagcgaa aggaagacga tcgctttcga ccaggacagc
    16561 cttactggaa tcgatggtgt ctccggtgtg aaagaacacc aacaggggtg ttaccactac
    16621 cgcaggaaaa ggaggacgtg tggcgagaca gcgacgaagt atcaccgaca taatctgtga
    16681 aactgcaaat accttccaac gaaacgctcc agaaataaac gcaagccaat cccaaaagaa
    16741 tctgacgtaa aaaccttcaa ctacacggct cacctgtggg atatccggtg gctaagacat
    16801 cgtgcgagga aatgacaatg cttttaattc aacctggatt tggacttagc atcaaaaaag
    16861 gccacatgtt tggcgagaaa gagtcacaac gaaaaatgat gtctatccgg ttgccattta
    16921 tcagtattta ttggctaaac agggaggcaa caaattattg gtatacatgc gccagagcag
    16981 catttaacga ccctgactgg tttgtggaaa accaccacgc agttcgtcag gcaaagagaa
    17041 aggccaacat gacatacatg aaggcgtatc gaaaagcatg gaaagaacac cgcgatcgat
    17101 accagcaaga tatggaaaag cttgaatcag aaaacatgga attaagacga aagcttggtg
    17161 aagcaaaacg agacattgat gcttacaagc gactttttaa tggtgaaagc catgcttagt
    17221 ccatcccaat ctcttcaata ccagaaagaa agcgtcgagc gggctttaac gtgcgctaac
    17281 tgcggtcaga agctgcatgt gctggaagtt cacgtgtgtg agcactgctg cgcagaactg
    17341 atgagcgatc cgaatagttc aatgtacgag gaagaagacg atggccaaac cagcgcgaag
    17401 acgatgtaaa aacgaagaat gtcgggaatg gtttcaccct gcattcgcta atcagtggtg
    17461 gtgctctcca gagtgtggaa ccaagatagc actcgaacga cgaagtaaag aacgcgaaaa
    17521 agcggaaaaa gcagcagaga agaaacgacg acgagaggag cagaaacaga aagataaact
    17581 taagattcga aaactcgcct taaagccccg cagttactgg attaaacaag cccaacaagc
    17641 cgtaaacgcc ttcatcagag aaagagaccg cgacttacca tgtatctcgt gcggaacgct
    17701 cacgtctgct cagtgggatg ccggacatta ccggacaact gctgcggcac ctcaactccg
    17761 atttgatgaa cgcaatattc acaagcaatg cgtggtgtgc aaccagcaca aaagcggaaa
    17821 tctcgttccg tatcgcgtcg aactgattaa ccgcatcggg caggaagcag tagacgaaat
    17881 cgaatcaaac cataaccgcc atcgctggac tgtcgtagag tgcaaggcga tcaaggcaga
    17941 gtaccaacag aaactcaaag acctgcgaaa tagcagaagt gaggccgcat gacgttctca
    18001 gtaaaaacca ttccagacat gctcgttgaa gcatacggaa accagacaga agtagcacgc
    18061 agactgaaat gtagtcgcgg tacggtcaga aaatacgttg atgataaaga cgggaaaatg
    18121 cacgccatcg tcaacgacgt tctcatggtt catcgcggat ggagtgaaag agatgcgcta
    18181 ttacgaaaaa attgatggca gcaaataccg aaatatttgg gtagttggcg atctgcacgg
    18241 atgctacacg aacctgatga aaaaactgga gacgatagga ttcgacacca aaaaagacct
    18301 gcttatctcg gttggcgatt tggtcgatcg cggtacagag aacgtcgaat gcctggaatt
    18361 aatcacattc ccctggttca gagctgtacg tggaaaccat gagcaaatga tgattgatgg
    18421 cttatcagag cgtggaaacg tcaatcactg gctacttaat ggcggcggct ggttctttaa
    18481 tatcgattac gacaaagaaa ttctggctaa agctctttcc cataaagcag atgaacttcc
    18541 gttaatcatc gaactggtga gcaaagataa aaaatatgtc atctgccacg ccgattatcc
    18601 ttgtgacgaa tacgagtttg gaaagccagt tgatcatcag caggtaatct ggaaccgcga
    18661 acgaatcagc aactcacaag acgggatcgt gaaagaaatc aaaggcgcgg acacgttcat
    18721 ctttggtcat acgccagcag tgaaaccact caaatttgcc aaccagatgt atatcgatac
    18781 cggcgcagtg ttctgcggaa acctcacatt gattcaggta cagggagaag gcgcatgaga
    18841 ctcgaaagcg tagctaaatt tcattcgcca aaaagcccga tgatgagtga ctcaccacgg
    18901 gccacggctt ctgactctct ttccggtact gatgtgatgg ctgctatggg gatggcgcaa
    18961 tcacaagccg gattcggaat ggctgcattc tgcggtaagc atgaactcag ccagaacgac
    19021 aaacaaaagg ctatcaacta tctgatgcaa tttgcacaca aggtatcggg gaaataccgt
    19081 ggtgtggcaa agctcgaagg aaatactaag gcaaaggtac tgcaagtgct cgcaacattc
    19141 gcttatgcgg attattgccg tagtgccgcg acgcctggcg caagatgcag agattgccac
    19201 ggtacaggtc gtgcggttga tatagccaaa acagagcagt gggggagagt tgtcgagaaa
    19261 gagtgcggaa gatgcaaagg cgtcggctat tcaaggatgc cagcaagcgc agcatatcgc
    19321 gctgtaacga tgctaatccc aaaccttacc caacccacct ggtcacgcac tgttaagccg
    19381 ctgtatgacg ctctggtggt gcaatgccac aaggaagagt caatcgcaga caacattttg
    19441 aatgcggtca cacgttagca gcatgattgc cacggatggc aacatattaa cggcatgata
    19501 ttgacttatt gaataaaatt gggtaaattt gactcaacga tggataaatg cactcgttaa
    19561 ataaagccct gagttaatag ctcggggctt tttgcgtttt aagcacggcc tttctgaaag
    19621 cacatcaagc caaataccag acagacaaaa ataatcacct tatccgcttg gctacggtgc
    19681 ggtgtgcttt gcataaaaga aaaccagcgc aatggctggc ttcgtgaaag cgggtggcaa
    19741 gaggctgcgc taacaacctc atgccgtttt gcccgtgcat atcggtcacg aacaaatctg
    19801 attactaaac acagtagcct ggatttgttc tatcagtaat cgaccttatt cctaattaaa
    19861 tagagcaaat ccccttattg ggggtaagac atgaagatgc cagaaaaaca tgacctgtta
    19921 gccgccattc tcgcggcaaa ggaacaaggc atcggggcaa tccttgcgtt tgcaatggcg
    19981 taccttcgcg gcagatataa tggcggtgcg tttacaaaaa cagtaatcga cgcaacgatg
    20041 tgcgccatta tcgcctggtt cattcgtgac cttctcgact tcgccggact aagtagcaat
    20101 ctcgcttata taacgagcgt gttcatcggc tacatcggta ctgactcgat tggttcgctt
    20161 atcaaacgct tcgctgctaa aaaagccgga gtagaagatg gtggaaatca ataatcaacg
    20221 taaggcgttc ctcgatatgc tggcgtggtc agagggaaca gataacggac gacagaaaac
    20281 cagaaatcat ggttatgacg tcattgtagg cggagagcta tttactgatt actccgatca
    20341 ccctcgcaaa cttgtcacgc taaacccaaa actcaaatca acagcagccg ggcgctatca
    20401 gcttctttcc cgttggtggg atgcctaccg taagcagctt ggcctgaaag acttctctcc
    20461 gaaaagccag gacgctgtgg cattgcaaca gattaaagag cgtggcgctt tgccgatgat
    20521 tgatcgcggt gatatccgtc aggcaatcga ccgttgcagc aatatctggg cttcgttgcc
    20581 cggggctggt tatggccagt tcgagcataa ggctgacagc ctgattgcaa aattcaaaga
    20641 agcaggcgga acggtcagag agattgaggt atgagcagag taaccgcgat tatctccgct
    20701 ctggttatct gcatcatcgt ctgcctgtca tgggctgtta atcattaccg tgataacgcc
    20761 atcgcctaca aagagcagcg cgacaaagcc gcatccacta tcgctgatat gcagaagcgt
    20821 caacgtgacg tagcagaact cgacgccaga tatacaaagg agcttgctga tgctaacgcg
    20881 actatcgaaa gcctccgtgc tgatgtttct gctggtcgta agcgcctgca agtcgccgcc
    20941 acctgtgcaa agtcaacgac cggagccagc agcatgggcg atggagaaag cccaagactt
    21001 acagcagatg ctgaactcaa ttattaccgt ctccgaagcg gaatcgacag gataaccgcg
    21061 caggttaact acctgcagga atacatcagg actcagtgcc tgaaataatt tttttgcaaa
    21121 tcacaaagtc catttaatga gcctcgcgat gcggggcttt tttacatctg aatttcacag
    21181 cgcatctcac gcgcatatta catcacccga gcctttcaga aagttgagcc tgagaactgc
    21241 cgtatatggt ggcgaccatc tcgggacggc ttttctgtga gacaggctca ctttctaaaa
    21301 ggtaaagacg caatgaacta cccaaccgtt gttaacgata tagatttcag agacctaatt
    21361 tttgtagcaa acaacgatcc ggttacagat tcttttatgg tggcaaaagc atttggaaag
    21421 ctgccgaaga acgtagttcg tgacattgag cgaaccatag aagcttgccc tcctgagttt
    21481 gatacaaagc tcaactttga gctttgctat aaaaacaatg agttacagaa tggtaagccg
    21541 caaaaattct accgtctccg caaggatggg ttgatgcttt tggttatgtc ctacaccaaa
    21601 aaagaagcaa tgcgtatcaa aattgcttac atcaacgcat ttaactggat gtacgccatg
    21661 cttcaggttg gtcatcgtca atttgaagaa gagagaaatg ccgtaatgct ggagtacatg
    21721 aaagagaagg atgttgccag catgtcaggt cgcctgctaa atcgctgggg caaaattaag
    21781 aagccacagc tgatggctag aattgaacgc cttgaacagc acgggcaaac cgtaatcccc
    21841 ggactcatca attaacggca gcaccgcgaa acaacccaag ccagtaagtg gggaaataac
    21901 actggcagcc actgaaagat gaacctccag ccttatggca aaaaagattc tttgtggtgg
    21961 cggactgatg gaaagacatc ggatagaatc aagcagtggc tagggtagct cccgaaaagc
    22021 ggactcgtca ccgcctgcca ctgaatctat ggcgaacaac tagacgaggt tgtgatggat
    22081 gataacggaa accaatgggt caatgttgaa caccggctac cagaaagcaa agaagggatg
    22141 tggtctaaag aggttatagc tcttactgat actggtgacg tgttcaaact atcatgtatg
    22201 ggctcttact ggcagagaac caaggcattc atcgattcag gagcaagcaa ggttacacac
    22261 tggatgccgc ttaattaccc agatgattaa aacggatgaa aaatgaaagg tcgctcaggc
    22321 ggcctttttt attgccatca caaaagccat tccttactga gtggctttga taatggctta
    22381 taccctacac gggataactt aactgatatc ccttttaacg gataaatgga gccaacaatg
    22441 gcagagatta ttcccatgac tgaagaacag aaattccagt tagagattta caaactggtc
    22501 atgaaccaga acgcagccgc agaagaagca tttcaattca ttggcactga cgaactgaag
    22561 cttgagctat tcaaaattca cttccagtca ggtggcgcta attcagatat cacgatccgc
    22621 acatttgaag cggtgcgtaa atcgaaggaa gcgttagacc tgttcaccac cggagcatga
    22681 tgctcaacct gaaataacga ttaagtgaga tgaatatggc agcaccaaag ggcaaccgat
    22741 tctgggaggc ccgcagtagt catgggcgaa accctaaatt cgaatcgcct gaggcgctgt
    22801 gggctgcttg ttgtgaatac ttcgagtggg ctgatgataa cccgctatgg gagggtaagg
    22861 tattttcata tcagggagaa ataattaagg ctaatgtccc taagatgcga gccatgacta
    22921 tttcaggatt gtgtaccttc cttgatatca ccaggcaaac atggggaacc ttccggtcaa
    22981 tggaaggttt ttctgacgtc acatcacgag cggaagacat catctacgac cagaaattct
    23041 ctggcgcagc cgctgacctt ctcaacgcta acatcatcgc ccgtgatttg ggcctcaaag
    23101 agcagtcgca agttgaagac gtgacacctg ataagggaga tcgcgataag cggcgctctc
    23161 gtatcaagga gctattcaac cgtggaactg gacgcgattc ttgataactt gagcgacgaa
    23221 gagcaaatcg aattgctcga gctactcgaa gaagaagaga actaccgtaa cacacacctg
    23281 ctatatgaat ttacgccata cagcaaacag cgtgagttca tcgacgccgg gcatgactat
    23341 ccagagcgct gttttatggc tggtaaccag cttggtaagt catttactgg tgctgctgaa
    23401 gtcgcgtttc accttaccgg gcgttatccg ggcacaaaag gctatcctgc tgatggtaaa
    23461 tatggcggtg agtggaaagg taagcgtttt tatgagcctg ttgtcttttg gattggtggc
    23521 gagacaaacg agactgtaac caaaacgact caacgcatcc tgtgtggtcg tatcgaagag
    23581 aatggtgagc caggctacgg ttccatacct aaagaagaca tcattagctg gaagaagtct
    23641 cctttctttc cgaaccttgt tgatcacctt ctggttaagc atcacacggc tgatggcgtt
    23701 gaagatggca tttcaatctg ctacttcaag ccatactcgc aaggtcgtgc tcgctggcag
    23761 ggtgacacaa tccacggcgt gtggtttgac gaagagccac catacagcat ttatggtgaa
    23821 ggccttaccc gtaccaacaa atacgggcaa ttctcaattc tgacgtttac cccgctgatg
    23881 gggatgtctg acgttgttac caagttcctg aagaatccaa gcaagtcgca gaaagtggtc
    23941 aacatgacca tctacgacgc tgagcactac acagacgaac agaaagagca aatcatcgca
    24001 tcttatcctg agcatgagag agaggcgcgt gctcgcggta ttcctacgat gggtagtggt
    24061 cgaatcttcc agataccgga agagacgatt aagtgtcagc cgttcgagtg tcctgatcac
    24121 ttctacgtaa ttggcgggat ggatttcgga tgggatcacc cgcaggcgca ggttcagctt
    24181 tggtgggaca aggacgcaga cacaatatac gtttcacgcg tgtggaaggc gaaagaaaaa
    24241 acagccgttc aggcgtgggg agccgttaaa tcatgggcgc ataaagtgcc aactgcatgg
    24301 cctcatgacg gaaaccagca cgagaagggc ggcggtgagc agctcaaagg gcagtatgca
    24361 gatgctggtt ttatgatgtt gcaggagcat gcgacatggc ctgatggcgg taatgctgtg
    24421 gagcctggca tcactgaatt gcgcgacatg atgctcgatg gtcgcttcaa agtattcaac
    24481 acctgtgagc cattctttga ggagttccgc ctctatcacc gtgatgaaaa cgggaaaatc
    24541 gtcaagctta acgacgacgt tctctcagcc gttcgctatg catacatgat gcgccgcttc
    24601 gccaaaatga tgcgcgacat caaaaaacca aaagagaaaa agataccagc cccaatcagg
    24661 cccatcgcac ggagaactta aatggccgac gaaaacagac tcaattccat tctgtgtaag
    24721 tttgacgcgg actggatggc gagcgatgaa gccagaaccg aggcgacaaa tgacctgtat
    24781 tttagccgag tgtcgcaatg ggatgactgg ctatcaaact acactaccct gcaatatcgc
    24841 ggac
//
LOCUS       DAJTRM010000059        24304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRM010000059 DAJTRM010000000
VERSION     DAJTRM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.059"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..386)
                     /locus_tag="OO322_002510"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO322_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO322_002511"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO322_002511"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5907345.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO322_002512"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO322_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5907346.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO322_002513"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO322_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5907347.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO322_002514"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO322_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5907348.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO322_002515"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO322_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5907349.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO322_002516"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO322_002516"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5907350.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO322_002517"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO322_002517"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5907351.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO322_002518"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO322_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5907352.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO322_002519"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO322_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5907353.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO322_002520"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO322_002520"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5907354.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO322_002521"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO322_002521"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5907355.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO322_002522"
     CDS             10748..11212
                     /locus_tag="OO322_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5907356.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO322_002523"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO322_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5907357.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO322_002524"
     CDS             12236..13672
                     /locus_tag="OO322_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5907358.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO322_002525"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO322_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5907359.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO322_002526"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO322_002526"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5907360.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO322_002527"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO322_002527"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5907361.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO322_002528"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO322_002528"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5907362.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO322_002529"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO322_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5907363.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO322_002530"
     CDS             complement(20505..20798)
                     /locus_tag="OO322_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5907364.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO322_002531"
     CDS             complement(20795..22084)
                     /locus_tag="OO322_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5907365.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO322_002532"
     CDS             complement(22140..23078)
                     /locus_tag="OO322_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5907366.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO322_002533"
     CDS             complement(23098..23862)
                     /locus_tag="OO322_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5907367.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         6009 a         5879 c         6391 g         6025 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gaca
//
LOCUS       DAJTRM010000060        24155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRM010000060 DAJTRM010000000
VERSION     DAJTRM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.060"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(22..594)
                     /locus_tag="OO322_002534"
     CDS             complement(22..594)
                     /locus_tag="OO322_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5907368.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO322_002535"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO322_002535"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5907369.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO322_002536"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO322_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5907370.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO322_002537"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO322_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5907371.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO322_002538"
     CDS             complement(3556..5337)
                     /locus_tag="OO322_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5907372.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO322_002539"
     CDS             complement(5330..7102)
                     /locus_tag="OO322_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5907373.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO322_002540"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO322_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5907374.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO322_002541"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO322_002541"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5907375.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO322_002542"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO322_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO322_002543"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO322_002543"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5907376.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO322_002544"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO322_002544"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5907377.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO322_002545"
     CDS             complement(11658..12029)
                     /locus_tag="OO322_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5907378.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO322_002546"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO322_002546"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5907379.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO322_002547"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO322_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5907380.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO322_002548"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO322_002548"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5907381.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO322_002549"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO322_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5907382.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO322_002550"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO322_002550"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5907383.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO322_002551"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO322_002551"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5907384.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO322_002552"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO322_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5907385.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO322_002553"
     CDS             21800..22378
                     /locus_tag="OO322_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5907386.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO322_002554"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO322_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5907387.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5663 a         6654 c         5825 g         6013 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaag
//
LOCUS       DAJTRM010000061        24091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRM010000061 DAJTRM010000000
VERSION     DAJTRM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.061"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="OO322_002555"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="OO322_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5907388.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO322_002556"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO322_002556"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5907389.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="OO322_002557"
     CDS             1533..2480
                     /locus_tag="OO322_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5907390.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="OO322_002558"
     CDS             2550..3356
                     /locus_tag="OO322_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5907391.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="OO322_002559"
     CDS             3366..3773
                     /locus_tag="OO322_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5907392.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3773..4267
                     /locus_tag="OO322_002560"
     CDS             3773..4267
                     /locus_tag="OO322_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5907393.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="OO322_002561"
     CDS             4333..5130
                     /locus_tag="OO322_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5907394.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="OO322_002562"
     CDS             5141..5929
                     /locus_tag="OO322_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5907395.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="OO322_002563"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="OO322_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5907396.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="OO322_002564"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="OO322_002564"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5907397.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="OO322_002565"
     CDS             complement(8372..10003)
                     /locus_tag="OO322_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5907398.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO322_002566"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO322_002566"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5907399.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="OO322_002567"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="OO322_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5907400.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15247..17431
                     /gene="espL4"
                     /locus_tag="OO322_002568"
                     /pseudo
     CDS             15247..17431
                     /gene="espL4"
                     /locus_tag="OO322_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="OO322_002569"
                     /pseudo
     CDS             complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="OO322_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="OO322_002570"
     CDS             complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="OO322_002570"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5907401.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="OO322_002571"
                     /pseudo
     CDS             complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="OO322_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22525..23454)
                     /gene="metA"
                     /locus_tag="OO322_002572"
     CDS             complement(22525..23454)
                     /gene="metA"
                     /locus_tag="OO322_002572"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5907402.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23611..24054
                     /locus_tag="OO322_002573"
     CDS             23611..24054
                     /locus_tag="OO322_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5907403.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6147 c         6109 g         5891 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatcg cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaaa agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtggaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg cagggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggtgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctggtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcacacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggtggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgcacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca gattctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcatg gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgaggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgcttaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tggcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 ccggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa tcgcaagatc tcattccacc
    15121 gaaactttta cggctgcatt atgagattga gcgatttatg atattttttt agttgtaatc
    15181 atattaaatt ttttcactgt tctgatagtt aaaattcaag acatcaataa acaatgagat
    15241 atttaaatga ttactcgtat tcctcgtagt tctttctctg caaatattaa taatacagcc
    15301 cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt
    15361 tctggcaagg agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat
    15421 gttcgtcata tctttagcaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa
    15481 gctgataaga aagaaattac gatgattaat aaaaacgaaa acacgacact cactcaaacc
    15541 attgctacaa tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt
    15601 gataaacaag aattaaacca aaaatcagat agaaaagaaa aagaattccc aagaattaaa
    15661 cttaatggtc aatgttattt cccggggcgc cccaaaaccg tatagtatac cgacacattg
    15721 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
    15781 actcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
    15841 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
    15901 ttatgaaagc atattttgac aagatggaat caaatggcat cagtgttatg gcagccatat
    15961 tactggtgga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
    16021 gtacccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatta
    16081 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
    16141 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
    16201 cacttccgaa agaacagcta ttgatgttaa aaggaactgt ggatgaaata acccctccat
    16261 tatcaccagc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
    16321 tgatgattca gctccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
    16381 atagtattaa cctccccggt ttatatttgg ctataaatta tggcaatgcg gatatcgttg
    16441 agactatttt caattcattg tcagaaacag gatatgaagg gttactctcg aaaaaaaatc
    16501 tcatgcatat tctggaggca aaagataaaa atggtttttc tggattattt ttagcgatat
    16561 cacgtaagga taaaaatgtt gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa
    16621 cacatcattt agataatgaa caggtgtata aattcctgag tgccaaaaat agaacgtcca
    16681 gccatgtttt ataccatgtt atggcgaatg gtgatgccga catgctgaaa atttttttgg
    16741 tcgcgttacc tttgttaatt cgcacatgtc atttgactaa agaacaggta ctcgatctcc
    16801 tgaaggcaaa ggatttttat ggttgcccaa gactatacct ggcgatgcaa aatggacata
    16861 gcgatatcgt gaaagttatt ctcgaagcat tgccctgtct ggcccaggaa attaacattt
    16921 cagcttccga tattgtagat cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt
    16981 ttatggccat gcagcgcgga catatgaacg ttattaatac tatttttaac gcattaccca
    17041 ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa
    17101 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga
    17161 gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
    17221 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttttga
    17281 atttggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
    17341 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
    17401 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
    17461 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
    17521 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
    17581 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
    17641 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
    17701 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
    17761 agcatgtcgc cagggaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
    17821 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
    17881 atccgccgct cgattataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt
    17941 ttccgctgct tcctggagca gtaattccat taataccggg gaaatatgcc gcttctccag
    18001 cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac
    18061 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
    18121 tttgatgact ttgaataccc gatcaaagcc cggcagcgta aacaccaaca tcaccattcc
    18181 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
    18241 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt
    18301 ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata
    18361 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
    18421 gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa
    18481 cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag
    18541 ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag
    18601 atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag
    18661 gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc
    18721 cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gagctaaaga taaaaagccg
    18781 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
    18841 cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag
    18901 taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag
    18961 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
    19021 gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
    19081 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
    19141 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
    19201 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
    19261 attgccggat gcggcgtaaa tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc
    19321 ttagaactgc gattcttcag tggagccagt cagcgcggtg actgaagacg tgccgccctg
    19381 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
    19441 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa catagtgctt
    19501 cataccttcg ccctgcgcat aagcgtttgc caggtcgaac atgttaaacc acatgctgtg
    19561 gatacctgct agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
    19621 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
    19681 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
    19741 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
    19801 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
    19861 gccggtaata aattcgctgt catacgggtc gcaatctgag gtgatcagat ccgccgcatc
    19921 agcatcggta cgtgcaacca gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc
    19981 cgcgaccagt ttctgaatag cttcctgagt tggcactaaa actttgccgc ccatgtgacc
    20041 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgcagcac cggcttcaat
    20101 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcatcggc
    20161 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccacggacca
    20221 ttggatctga tcggcacgac ggaaggtgtt gttgatccgc tccaccacag ctggcaccga
    20281 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
    20341 tacctgccat cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg
    20401 accgccagtc agtgcaccga ggttgttgat gtagcctttt ttcgactcac cgtgcagcag
    20461 acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg
    20521 taatttcacc acatcttccg cactgtatgg gcgagtaatg ccttcccaac gcggttgagt
    20581 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
    20641 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
    20701 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg gccctgggag
    20761 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg
    20821 aacaaggctt tggtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
    20881 cagatcgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
    20941 acgcagccgt tgccggagat ccacgcttcg atgtactgca cagccacgcg aatgttagcg
    21001 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
    21061 ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac gggagccgag aatgtcgttg
    21121 aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg
    21181 ttattggctt ccagcgcttt atccgctttt actttgttga gcacctggtt attacgctct
    21241 tcatctttgc tcggaataaa cgcggccatg ccgcccatcg caaaagcacc gcgtttatgg
    21301 caggttttaa tcagcaggcg tgagtaagca ttcaggaatg gtttatccat cgtcacggcc
    21361 tgtctgtctg gcaggacacg atcgggatag tttttcaacg ttttgatata gctgaagatg
    21421 taatcccagc gaccgcagtt cagaccaaca atatggtcgc gcagcgcgtg aaggatttcg
    21481 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
    21541 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
    21601 taggactggg ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga
    21661 tagttgtgga agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc
    21721 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
    21781 ttgggcttga gctgataaat tttgcctgct tcattggtat agctgatggt accgttaacc
    21841 gcatcgcgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
    21901 tcgaaatcgg ccataaagac tttcacattg gcgttcagtg cgttgatcac catcttgcgc
    21961 tctaccgggc cagttatctc tacgcggcgg tcttctaagt ccgcaggaat cccgcgaatt
    22021 ttccaatcag catcgcgaat ggaagccgtt tccgaaataa aatcaggcaa cgttccgtta
    22081 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
    22141 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
    22201 tcgccatacg gccttgtgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
    22261 cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa cgaaggaacg ttgtgcgctt
    22321 ttcgttcagc acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt
    22381 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacttgctg
    22441 aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
    22501 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
    22561 tggcgtgatc tgatagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
    22621 acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc gggaaatagt tatacggtac
    22681 atccgggtct agtccggctt ccacatcgcg taaatattcc tgcgccagcg tttgcgcatc
    22741 atattcggga tggcccgtca caaaggcaat gcgcttatct ttgctggcaa acagatatgc
    22801 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
    22861 cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa tcatcaaagc cacgcgtcag
    22921 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
    22981 ttgcttagga atgccgtaga ggatattgag cgcggcctgt accgcccagc agacaaacag
    23041 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
    23101 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
    23161 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
    23221 gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
    23281 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggatcag
    23341 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
    23401 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg
    23461 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
    23521 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt
    23581 agaatataag aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacggcat
    23641 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
    23701 ttatcagcag agtatcgggc cgagctggaa gagctggtgc gttcctttct gccggaagca
    23761 ccgttgtggg tcgcggttaa tgaacgggag cagccggttg ggtttatgtt gttaagtggg
    23821 cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg gctgcggcgt gggtcggatg
    23881 ctggtggagc atgcgctctc gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat
    23941 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
    24001 gacgatttgg ggaaaccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
    24061 tctgccgtgc ctgcccaagc tggagaagac g
//
LOCUS       DAJTRM010000062        22940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRM010000062 DAJTRM010000000
VERSION     DAJTRM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.062"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            76..852
                     /gene="exuR"
                     /locus_tag="OO322_002574"
     CDS             76..852
                     /gene="exuR"
                     /locus_tag="OO322_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5907404.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1197..1859
                     /locus_tag="OO322_002575"
     CDS             1197..1859
                     /locus_tag="OO322_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5907405.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1863..2246
                     /gene="mzrA"
                     /locus_tag="OO322_002576"
     CDS             1863..2246
                     /gene="mzrA"
                     /locus_tag="OO322_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5907406.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2393..2747
                     /locus_tag="OO322_002577"
                     /pseudo
     CDS             2393..2747
                     /locus_tag="OO322_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2785..3090
                     /locus_tag="OO322_002578"
     CDS             2785..3090
                     /locus_tag="OO322_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5907407.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3093..3497
                     /locus_tag="OO322_002579"
     CDS             3093..3497
                     /locus_tag="OO322_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5907408.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3487..3786
                     /locus_tag="OO322_002580"
     CDS             3487..3786
                     /locus_tag="OO322_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5907409.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3972..4268
                     /locus_tag="OO322_002581"
     CDS             3972..4268
                     /locus_tag="OO322_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5907410.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4338..5324
                     /gene="yqjG"
                     /locus_tag="OO322_002582"
     CDS             4338..5324
                     /gene="yqjG"
                     /locus_tag="OO322_002582"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5907411.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5617..5985
                     /locus_tag="OO322_002583"
     CDS             5617..5985
                     /locus_tag="OO322_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5907412.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6192..7088)
                     /gene="yhaJ"
                     /locus_tag="OO322_002584"
     CDS             complement(6192..7088)
                     /gene="yhaJ"
                     /locus_tag="OO322_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5907413.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7193..7894
                     /locus_tag="OO322_002585"
     CDS             7193..7894
                     /locus_tag="OO322_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5907414.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7917..8081
                     /locus_tag="OO322_002586"
     CDS             7917..8081
                     /locus_tag="OO322_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907415.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8293..9603)
                     /locus_tag="OO322_002587"
     CDS             complement(8293..9603)
                     /locus_tag="OO322_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5907416.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9631..10962)
                     /locus_tag="OO322_002588"
     CDS             complement(9631..10962)
                     /locus_tag="OO322_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5907417.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11237..12601)
                     /gene="tdcG"
                     /locus_tag="OO322_002589"
     CDS             complement(11237..12601)
                     /gene="tdcG"
                     /locus_tag="OO322_002589"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5907418.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12673..13062)
                     /locus_tag="OO322_002590"
     CDS             complement(12673..13062)
                     /locus_tag="OO322_002590"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5907419.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13076..15370)
                     /gene="tdcE"
                     /locus_tag="OO322_002591"
     CDS             complement(13076..15370)
                     /gene="tdcE"
                     /locus_tag="OO322_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5907420.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15404..16612)
                     /gene="tdcD"
                     /locus_tag="OO322_002592"
     CDS             complement(15404..16612)
                     /gene="tdcD"
                     /locus_tag="OO322_002592"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5907421.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16638..17969)
                     /gene="tdcC"
                     /locus_tag="OO322_002593"
     CDS             complement(16638..17969)
                     /gene="tdcC"
                     /locus_tag="OO322_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5907422.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17991..18980)
                     /gene="tdcB"
                     /locus_tag="OO322_002594"
     CDS             complement(17991..18980)
                     /gene="tdcB"
                     /locus_tag="OO322_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5907423.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19079..20017)
                     /gene="tdcA"
                     /locus_tag="OO322_002595"
     CDS             complement(19079..20017)
                     /gene="tdcA"
                     /locus_tag="OO322_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5907424.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20207..20551
                     /gene="tdcR"
                     /locus_tag="OO322_002596"
     CDS             20207..20551
                     /gene="tdcR"
                     /locus_tag="OO322_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5907425.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20807..21347
                     /locus_tag="OO322_002597"
                     /pseudo
     CDS             20807..21347
                     /locus_tag="OO322_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21369..21716
                     /locus_tag="OO322_002598"
     CDS             21369..21716
                     /locus_tag="OO322_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907426.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21885..22535
                     /locus_tag="OO322_002599"
     CDS             21885..22535
                     /locus_tag="OO322_002599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907427.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6187 a         5782 c         5290 g         5681 t
ORIGIN      
        1 tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca
       61 ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgctgct
      121 gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact gcctgccgaa
      181 cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag
      421 gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag
      541 aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca taacccgtac
      601 tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg tgatgaccac
      661 gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg
      721 cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga tttcgagttc
      781 aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac ggccaccagc
      841 ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt ctcgtcggta
      901 agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcaatcag
      961 caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa
     1021 ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt taacagggaa
     1081 tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg
     1141 cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg
     1201 aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc
     1261 catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaatggct
     1321 tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg
     1381 cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg
     1441 gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact
     1501 ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg
     1561 gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg ctgccgacga
     1621 ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg atgagcggtc
     1681 tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt
     1741 taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt
     1801 ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag
     1861 ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc
     1921 tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga
     1981 ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg
     2041 acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat
     2101 ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg
     2161 gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac
     2221 gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg
     2281 tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt
     2341 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
     2401 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
     2461 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
     2521 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
     2581 tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga
     2641 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa cggaaactgg
     2701 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
     2761 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2821 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
     2881 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     2941 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct
     3001 gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta
     3061 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3121 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3181 aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac
     3241 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3301 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3361 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     3421 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg
     3481 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3541 cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc aacaggcgct
     3601 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtaga
     3661 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3721 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     3781 ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt ttgaatattt
     3841 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     3901 gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     3961 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac attttaatgc
     4021 cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat
     4081 atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta tttcacagca
     4141 actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat
     4201 tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg ctgaataaaa
     4261 agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc
     4321 tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac acctggtacg
     4381 ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca
     4441 ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt
     4501 atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac
     4561 tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg
     4621 gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat
     4681 ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg
     4741 tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa atcatccgca
     4801 tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac ccaccagccc
     4861 tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt
     4921 ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg
     4981 aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc
     5041 taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca gtgtatgtga
     5101 cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat ggcttcctgc
     5161 gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac atccgtaatc
     5221 attacttccg cagccataag accatcaacc ctacggggat tatttcaatt ggtccgtggc
     5281 aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg
     5341 aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct
     5401 tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag gccagataag
     5461 cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc acggcacttt
     5521 attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc
     5581 tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtacta
     5641 aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg
     5701 gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc
     5761 gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt accgtggtgg
     5821 gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgctattc
     5881 ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca
     5941 ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt aagcaccttg
     6001 taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg cggcgtaaac
     6061 gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc gtaggcctga
     6121 taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc ttgtgaaagg
     6181 tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca ccaggatttc
     6241 gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct ggtcgattcc
     6301 gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg atacggcatc
     6361 gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt
     6421 aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc
     6481 gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc cggctcctga
     6541 tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag cttgcgcgag
     6601 ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc cgcccgcccc
     6661 tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag ttgggtattg
     6721 gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg
     6781 gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc agtagtcagt
     6841 ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc gacattggtg
     6901 aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc ttccagtttt
     6961 tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc cgccgcaaaa
     7021 ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa tgccctttct
     7081 ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat ctggctaaca
     7141 atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa ttatgattac
     7201 tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg cccggtatac
     7261 tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct
     7321 taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc ccaaagtcga
     7381 tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag gcaatcatgt
     7441 tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca gctatagcga
     7501 acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg atacctgccc
     7561 gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc agcaattaat
     7621 cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt ggctgcacca
     7681 tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac gcgcgtattt
     7741 gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag cggcgctaac
     7801 ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg ccgattcccc
     7861 actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag atgatgatga
     7921 gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa gaacctgctc
     7981 gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct atcgtcgcgg
     8041 atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt gccggatgcg
     8101 gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa acaccccgta
     8161 ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca acaacatcaa
     8221 caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg cacaatactc
     8281 aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc gatccgtttg
     8341 ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa catcatgcgc
     8401 cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca ccgctttcca
     8461 cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat
     8521 catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt cataacgccc
     8581 atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg cggcacaaag
     8641 tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag aaagcatcag
     8701 cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca ccacaggcat
     8761 tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa gcgtagcgcc
     8821 gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag agagatcttt
     8881 cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca gcccccactt
     8941 accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg
     9001 gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga
     9061 aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt gggtaaacac
     9121 cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca
     9181 cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc
     9241 ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg taattgcctg
     9301 cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag
     9361 cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt
     9421 cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc
     9481 cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt
     9541 tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa
     9601 catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca
     9661 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
     9721 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
     9781 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
     9841 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
     9901 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
     9961 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
    10021 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
    10081 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
    10141 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
    10201 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
    10261 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
    10321 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
    10381 cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga ataacaattt
    10441 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
    10501 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
    10561 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
    10621 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
    10681 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
    10741 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
    10801 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
    10861 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
    10921 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt
    10981 ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg
    11041 caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc
    11101 aagactttaa aataacggag atggataaga attttctact aaattaatca caagagaata
    11161 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
    11221 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
    11281 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
    11341 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
    11401 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
    11461 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
    11521 tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg ccaatctccc
    11581 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
    11641 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
    11701 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
    11761 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
    11821 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
    11881 cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
    11941 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc
    12001 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
    12061 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
    12121 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
    12181 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
    12241 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
    12301 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
    12361 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
    12421 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
    12481 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
    12541 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
    12601 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
    12661 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
    12721 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
    12781 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
    12841 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
    12901 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
    12961 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
    13021 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
    13081 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
    13141 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
    13201 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
    13261 catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg
    13321 gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg
    13381 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
    13441 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
    13501 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
    13561 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
    13621 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
    13681 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
    13741 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
    13801 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
    13861 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
    13921 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
    13981 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
    14041 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
    14101 agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat
    14161 actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta
    14221 attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg
    14281 tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc
    14341 cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac
    14401 gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct
    14461 gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg
    14521 tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt
    14581 agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt
    14641 tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca
    14701 gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt
    14761 gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac
    14821 cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc
    14881 agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat
    14941 cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt
    15001 taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca
    15061 ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg
    15121 tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt
    15181 ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat
    15241 cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc
    15301 agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat
    15361 ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg
    15421 gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg
    15481 gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt
    15541 tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga
    15601 ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca
    15661 gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga acaaaggttt
    15721 taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat
    15781 ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt
    15841 cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc
    15901 cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac
    15961 tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc
    16021 cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg
    16081 acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc
    16141 cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct
    16201 gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat
    16261 gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg
    16321 cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt
    16381 catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt
    16441 ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa
    16501 taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt acggaaaact
    16561 taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc
    16621 tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca
    16681 atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta
    16741 cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct
    16801 tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg
    16861 aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa
    16921 ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa
    16981 gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac
    17041 gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata
    17101 ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca
    17161 aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta
    17221 cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag
    17281 gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca
    17341 gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata
    17401 actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac
    17461 ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa
    17521 gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc
    17581 tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt
    17641 gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc
    17701 tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc
    17761 agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg
    17821 atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta
    17881 ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg
    17941 ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca
    18001 acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg
    18061 acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc
    18121 agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt
    18181 ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa
    18241 gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc
    18301 gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg
    18361 ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa
    18421 ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc
    18481 agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata
    18541 cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca
    18601 tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct
    18661 tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc
    18721 gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa
    18781 cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac
    18841 ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt
    18901 ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc
    18961 agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg
    19021 taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaacaact
    19081 aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac tctttggcta
    19141 attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat
    19201 attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag
    19261 gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca agattataaa
    19321 ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg
    19381 tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct
    19441 tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa
    19501 actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg
    19561 taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg
    19621 cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg
    19681 acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcttcag
    19741 aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac
    19801 gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta
    19861 attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta
    19921 accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta
    19981 ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca
    20041 ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca ctcacaaatt
    20101 aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa tatcaacaag
    20161 ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga cggggataac
    20221 catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc
    20281 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
    20341 gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat
    20401 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
    20461 tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
    20521 gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag
    20581 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga
    20641 agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc
    20701 gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga
    20761 aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc
    20821 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
    20881 tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga
    20941 cgttaataaa atttacagta taagtaatga actccggaga gtattatctg caattactgc
    21001 attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa ccggaaaata
    21061 tgagtccatt catcatagat atttctacag gagaacatga tgattatatc atacaaacat
    21121 tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa
    21181 aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc acccaaaaag
    21241 aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca gattgttggc
    21301 aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg
    21361 ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc
    21421 gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag
    21481 gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac
    21541 caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg
    21601 taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac acccaaatta
    21661 ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa aactaggaaa
    21721 tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcagga
    21781 ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact
    21841 tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct aatactattc
    21901 taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt
    21961 ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa ataacgcctg
    22021 ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt
    22081 taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt gcaattataa
    22141 aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat
    22201 ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca gagatgaaag
    22261 aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat tatgaaaata
    22321 tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat ttaaacatag
    22381 aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc cggacaatgg
    22441 ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc aatattttac
    22501 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
    22561 tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa
    22621 cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
    22681 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
    22741 cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg
    22801 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtatagtgtc
    22861 gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt
    22921 acgtatatca ggtgaaactg
//
LOCUS       DAJTRM010000063        22564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRM010000063 DAJTRM010000000
VERSION     DAJTRM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.063"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..74
                     /locus_tag="OO322_002600"
     CDS             <1..74
                     /locus_tag="OO322_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2767 family protein"
                     /protein_id="HCR5907428.1"
                     /translation="NKRIQRSELEKQAMETVINALVK"
     gene            530..1156
                     /locus_tag="OO322_002601"
     CDS             530..1156
                     /locus_tag="OO322_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5907429.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1177..3278
                     /locus_tag="OO322_002602"
                     /pseudo
     CDS             1177..3278
                     /locus_tag="OO322_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3291..3929
                     /locus_tag="OO322_002603"
     CDS             3291..3929
                     /locus_tag="OO322_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5907430.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3993..4661
                     /locus_tag="OO322_002604"
     CDS             3993..4661
                     /locus_tag="OO322_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5907431.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4658..5443
                     /locus_tag="OO322_002605"
     CDS             4658..5443
                     /locus_tag="OO322_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5907432.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5447..6259
                     /locus_tag="OO322_002606"
     CDS             5447..6259
                     /locus_tag="OO322_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907433.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6271..7693)
                     /locus_tag="OO322_002607"
                     /pseudo
     CDS             complement(6271..7693)
                     /locus_tag="OO322_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7819..8124)
                     /locus_tag="OO322_002608"
     CDS             complement(7819..8124)
                     /locus_tag="OO322_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5907434.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8232..8308)
                     /locus_tag="OO322_002609"
     tRNA            complement(8232..8308)
                     /locus_tag="OO322_002609"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8272..8274),aa:Val,seq:gac)
     gene            complement(8313..8389)
                     /locus_tag="OO322_002610"
     tRNA            complement(8313..8389)
                     /locus_tag="OO322_002610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8353..8355),aa:Val,seq:gac)
     gene            8697..9953
                     /locus_tag="OO322_002611"
     CDS             8697..9953
                     /locus_tag="OO322_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5907435.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9994..11367)
                     /gene="mdtK"
                     /locus_tag="OO322_002612"
     CDS             complement(9994..11367)
                     /gene="mdtK"
                     /locus_tag="OO322_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5907436.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11582..12223
                     /gene="ribE"
                     /locus_tag="OO322_002613"
     CDS             11582..12223
                     /gene="ribE"
                     /locus_tag="OO322_002613"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5907437.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12263..13411)
                     /gene="cfa"
                     /locus_tag="OO322_002614"
     CDS             complement(12263..13411)
                     /gene="cfa"
                     /locus_tag="OO322_002614"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5907438.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13702..14913)
                     /locus_tag="OO322_002615"
     CDS             complement(13702..14913)
                     /locus_tag="OO322_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5907439.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15026..15958
                     /locus_tag="OO322_002616"
     CDS             15026..15958
                     /locus_tag="OO322_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5907440.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(15955..16980)
                     /gene="purR"
                     /locus_tag="OO322_002617"
     CDS             complement(15955..16980)
                     /gene="purR"
                     /locus_tag="OO322_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5907441.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17279..17368
                     /gene="ynhF"
                     /locus_tag="OO322_002618"
     CDS             17279..17368
                     /gene="ynhF"
                     /locus_tag="OO322_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5907442.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17534..18703
                     /locus_tag="OO322_002619"
     CDS             17534..18703
                     /locus_tag="OO322_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907443.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18849..19430)
                     /gene="sodB"
                     /locus_tag="OO322_002620"
     CDS             complement(18849..19430)
                     /gene="sodB"
                     /locus_tag="OO322_002620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5907444.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19558..20385)
                     /gene="mepH"
                     /locus_tag="OO322_002621"
     CDS             complement(19558..20385)
                     /gene="mepH"
                     /locus_tag="OO322_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5907445.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20720..21067
                     /gene="grxD"
                     /locus_tag="OO322_002622"
     CDS             20720..21067
                     /gene="grxD"
                     /locus_tag="OO322_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5907446.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21106..21606)
                     /gene="tnpA"
                     /locus_tag="OO322_002623"
     CDS             complement(21106..21606)
                     /gene="tnpA"
                     /locus_tag="OO322_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5907447.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21614..>22564
                     /locus_tag="OO322_002624"
     CDS             21614..>22564
                     /locus_tag="OO322_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5907448.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLW"
BASE COUNT         5852 a         5265 c         5835 g         5612 t
ORIGIN      
        1 ccaataaacg cattcagcgc agtgagctgg aaaagcaggc gatggaaacc gtcattaacg
       61 cactggtaaa atgaccattc aggatgaggc ggtaagcttc atcctgatat tcttgcttca
      121 gttgcggtcc agttaatcat tctaaataac aactgctgaa attatacgaa tttcggtatg
      181 tattttaatt tatagcgata atgttatttt tattttctgc aatgaacgtc acaaatgatt
      241 gtcgcttaag ttaaatttat ttcaaatgat aataaatatc agttcgctta taatttcttg
      301 ctttgtaatt ttaattgata acgatcaatg ttaagggctc agaataatca cattattttt
      361 tctcctaacc ataaaggatt agtttatcgg cgttaaatct tttctcatac actatcgggt
      421 tagggaaaat atctggtgtt ggggacagag tcagcagcgt attagttgaa tgcttaatat
      481 caggctgctg aattgtcagc actgtgtttg cgaagaggat gcgaaataaa tgaacccggt
      541 tgatcgtcca ctattagata ttggcttaac gcgattagaa ttcttgcgta tatcaggaaa
      601 aggccttgct ggtctaacaa ttgcccctgc gttactgtca cttttgggtt gcaaacagga
      661 agatattgat agcggcacag tagggttgat aaatacaccc aaaggggtgc tggtgaccca
      721 gcgagcgcga tgcacgggtt gccaccgctg tgaaatctct tgtaccaact tcaacgatgg
      781 ctcagtaggg acattcttct cccgtatcaa aatccatcgc aattatttct ttggcgacaa
      841 cggggttggc tctggcggcg gcctgtatgg cgatctcaac tataccgcgg acacctgccg
      901 tcaatgcaaa gagccgcaat gcatgaacgt ctgcccgatt ggtgcgatta cctggcagca
      961 gaaagaaggc tgtattaccg tcgatcataa acgttgtatt ggctgtagcg cctgtactac
     1021 ggcatgcccg tggatgatgg ccaccgtaaa taccgaaagt aaaaaatcct cgaaatgtgt
     1081 gttatgcggt gaatgcgcaa acgcctgccc aacaggggcg ttaaaaatta tcgagtggaa
     1141 agatattact gtgtgaatct tgcaaaggaa aaaatcatgg ctaacggttg gacaggtaat
     1201 atattaagag tcaatctcac gacaggaaat attaccctcg aagattccag taagtttaaa
     1261 agttttgtcg gtggcatggg cttcggctac aaaattatgt atgacgaagt accgctaggc
     1321 acgaaacctt tcgatgaagc gaataaatta gtctttgcta ccggcccatt acctggatct
     1381 ggtgccccct gtagttctcg cgtaaatatc acctcacttt ctacttttac caaaggaaat
     1441 ttagtcgcga tgcccatatg ggtggctttt ttgcagcgca aatgaaattc gctggatacg
     1501 acgtcattat tatcgaaggg aaggcgaaat caccggtatg gctgaagatt aaagatgaca
     1561 aagttagcct ggaaaaagcc gatttcttat ggggaaaagg gacgcgcgca acgacggaag
     1621 aaatttgtcg attgaccagc ccggaaacct gtgtggcggc tattggtcag gctggggaaa
     1681 accttattcc tctctctggc atgttgaata gccgtaacca cagcggcggt gcgggaactg
     1741 gcgcaataat gggttcgaaa aacctgaaag cgattgcggt tgaagggacg aaaggggtca
     1801 acattgccga tcgtcaggag atgaagcgtc tcaatgatta catgatgact gaacttattg
     1861 gtgcgaataa caaccatgtc gtgccaagta cgccacaatc gtgggcagag tattcagatc
     1921 ccaagtcacg ctggacagca cgtaaagggc tgttttgggg cgcggctgaa ggtggtccga
     1981 ttgaaacggg tgaaattccg ccaggcaatc agaatacggt cggctttcgt acctataaat
     2041 ccgtttttga cttaggaccg gcggcagaga aatacacagt aaaaatgagc ggctgccact
     2101 cttgcccgat ccgttgtatg acccaaatga atattcctcg ggtgaaagag tttggcgtgc
     2161 ccagcacagg tggtaacact tgtgtagcaa actttgtcca taccaccatc ttcccgaacg
     2221 ggccgaaaga ttttgaagat aaagacgatg gtcgtgtgat tggtaacctg gttggtctga
     2281 atttgttcga tgactacggc ctatggtgta actacgggca gttgcatcgc gactttactt
     2341 attgttacag caaaggtgtg ttcaagcgtg ttctgccagc tgaagagtat gcagaaattc
     2401 gctgggatca actggaagcg ggtgacgtta acttcattaa agatttttac taccgtctgg
     2461 cgcatcgtgt gggtgagctg agtcacctgg ctgatggttc atatgccatc gcagaacgct
     2521 ggaatttggg taaagagtac tggggctacg cgaaaaataa actctggtcg ccgtttggct
     2581 atccggttca ccatgccaat gaagcgtcag cgcaggtcgg ttccattgtt aagtgtatgt
     2641 tcaaccgtga ctgcatgacg catacccata tcaactttat tggttccggc ttgccattga
     2701 aactgcaacg tgaagtggcg aaagaacttt ttggttctga agatgcttac gatgaaacca
     2761 aaaactacac gccaatcaat gacgcaaaaa ttaaatatgc aaagtggtcg ctgttgcggg
     2821 tctgtttgca taacgccgtc actctgtgca actgggtctg gccaatgacc gtttcgccgc
     2881 tgaaaagccg taattaccgg ggcgatctgg cgcttgaagc caaattcttc aaagcgatca
     2941 ccggcgaaga gatgactcag gaaaaattag atttagctgc agagcgtatc tttacgttgc
     3001 atcgtgccta cacggtaaaa ccgatgcaaa ccaaagatat gcgtaatgaa cacgatctta
     3061 tctgttcctg ggttttcgac aaggatccgc agatcccggt ctttactgaa ggtactgaca
     3121 aaatggatcg tgacgatatg catgcttcgc tcacgatgtt ctacaaagaa atgggctggg
     3181 acccacagct tggttgtcca acccgcgaaa cattgcagcg tctggggctg gaagatattg
     3241 ccgccgatct ggcagcacac aatctactgc ctgcgtaagg aatgggtaag atgaaccatc
     3301 aggatgaatt gccgttagcg aaagtcagcg aagttgacga ggcgaagcgg caatggttgc
     3361 aggggatgcg tcaccccgtt gatacggtaa ccgaaccaga acctgctgaa atcctggctg
     3421 agttcattcg ccagcattct gcggctgggc agctggtggc gcgcaccgtg tttctctcgc
     3481 caccctattc ggttgcagaa gaggaactgt cagttttgct ggaaagcatt aagcagaacg
     3541 gagactacgc ggatatcgct tgtatgacgg gaagtcagga tgattactat tactcaacgc
     3601 aggccatgag tgaaaactac gcggcgatgt ctttgcaagt tgtggagcag gacatttgtc
     3661 gcgccattgc acacgccgtt cgttttgagt gccagacgta tcctcgtcct tacaaagtcg
     3721 ccatgctgat gcaagcgcca tattacttcc aggaagcgca gattgaagcc gcgatcgccg
     3781 cgatggatgt tgcacctgaa tatgccgata tacggcaggt ggagtcatcg acggcggtgt
     3841 tgtacttgtt tagcgagcga tttatgacct atggcaaggc ttatggctta tgcgaatggt
     3901 tcgaggtaga gcagtttcag aacccctgaa atttcaggga gatgtcatgg atagggtgga
     3961 cagttgcagc cactgcatta ggggataatc agatgtcctt cactcgacgc aaatttgttc
     4021 tggggatggg aacagtaatc ttttttactg gttctgcgtc ttctctgtta gcgaacacga
     4081 ggcaagaaaa ggaagttcgg tatgccatga ttcatgacga gtcacggtgc aatggctgta
     4141 atatttgcgc ccgcgcatgc cgtaaaacta accacgtccc cgctcaggga agccgtttat
     4201 cgatagcaca cattccagtc accgataacg acaacgaaac gcagtatcac ttttttcgcc
     4261 agtcatgtca gcactgcgaa gatgctccgt gcatcgacgt atgcccgacg ggggcatcgt
     4321 ggcgtgatga gcaggggatc gtgcgggtag aaaaatcgca gtgcatcggc tgcagttatt
     4381 gcattggcgc ttgtccgtac ctggttcgct accttaaccc tgtcactaaa gtggcagata
     4441 aatgtgattt ctgcgctgag tcccgactgg caaaaggctt tccgcccatt tgcgtcagcg
     4501 cctgtccgga acacgcattg atctttggtc gcgaagatag cccagaaatt caggcgtggt
     4561 tgcaggataa taaatactat caatatcagc tacctggtgc cggaaaaccg catctgtatc
     4621 gccggttcgg tcaacatttg attaaaaagg aaaatgtatg aacccgtcgc aacatgctga
     4681 acagtttcag agccagttag cgaactatgt gccacagttc actcccgaat tttggccggt
     4741 gtggttgatc attgccggag tattgctggt tgggatgtgg ttggtgctgg ggctgcacgc
     4801 cttgcttcgt gctcgtggcg tgaagaaatc agccaccgat catggtgaga aggtttatct
     4861 ttacagcaaa gcggtcagat tatggcactg gtcgaatgcg ttactctttg tattgttgct
     4921 ggcaagtggg ctgataaatc actttgcgat ggtgggcgca actgcggtta aaagtctggt
     4981 tgcggtgcat gaagtttgcg gatttttgtt actggcatgc tggctcggct ttgtgctgat
     5041 caatgccgtt ggggataatg gtcaccacta tcgcattcgt cgtcaggggt ggctggaacg
     5101 agcggcaaaa caaacgcgat tttatttgtt tggcattatg cagggggaag aacatccttt
     5161 cccggcaaca acacagtcta aatttaatcc cttacagcag gtcgcctatg ttggtgtcat
     5221 gtatggattg ctgccgttgt tactattgac ggggctgttg tgtctctatc cgcaagccgt
     5281 gggagatgtg tttcctggcg taagatactg gttattgcag gcgcattttg ctctggcatt
     5341 tataagcctc ttttttatct tcggtcatct ttatctttgc accacggggc gtacgccaca
     5401 cgaaaccttt aaaagcatgg tcgatggcta tcaccggcac taacgaatga tcattacacg
     5461 agcagacctg cgagaatggc gtatcggagc ggtgatgtat cgctggttcc tgcgccattt
     5521 cccacgcggc ggtagttatg ccgatattcg tcatgcactg attgaggaag gatatacgga
     5581 ctgggcggag agtctggttg agtatgcgtg gaaaaaatgg ctggcggatg aaaacttcgc
     5641 gcatcaggaa gtttcctcta tgcaaaaact ggccacagac cccggagaga taccattttg
     5701 cagtcagttt gcccgcagcg acgatcatgc tcgcatcggc tgttgtgagg ataacgcccg
     5761 aatcgcaacc gctgggtatg ccgcgcaaat tgccagtatg ggttatagcg tgcggattgg
     5821 aagtgtcggc tttaacagtc acattggcag ctccggtgaa cgcgcacggg ttgctgtaac
     5881 cggtaattct tcgcggataa gcagcgctgg agatagtagt cggattgcga ataccggaat
     5941 gcgagtacgc gtttgtacat tgggtgaacg gtgtcatgtc gccagtaatg gtgacctggt
     6001 gcaaatcgcc agctttggcg caaacgccag aattgccaac agcggcgaca atgtacacat
     6061 cattgccagc ggtgaaaatt caacggtagt gagcacgggc gtggtcgact caattattct
     6121 cgggctaggg ggaagcgccg cactggcata tcatgacggc gaaagagttc gcttcgccgt
     6181 cgccattgaa ggtgaaaaca atattcgaac cggagtccgc tatcgtctta atgaacagca
     6241 tcagtttgtt gagtgctgat agctttacct ttaatcaaac gaaagacgcc gacgagcacg
     6301 ggatgcaggc tttacgaccg cccgagccat cgcctcacca atttctgcac atgccgtaag
     6361 tccctgaacg aaaggctggt cgtgcatcaa ccagggcaac gcgccgaacg ctacgcgtcc
     6421 acgaatatct tcgggttgct gtaacgtata atcttcgcca acatcaggga tttcagatcc
     6481 ccgcttcacg taaggccagt agtcgacgaa tagactctga agggattgcc gcataattat
     6541 cgatgaaaac cctggcaagg cctttactga accgtttatg gtcgtgttca ttcagatgag
     6601 gaacaatttc ctgtacggct tcatgcaggc gcagaatgac ataacgccag ggaacagtat
     6661 gtttttctcg tttattgcgt tcgacttcct ggagattttt ttctgcccag tcaaatgggt
     6721 cgcgttgttt gcgctcggca aaccaggctt gagcaaagga atcgacattc aggctctcta
     6781 aggctatgcg ttggctccag tctggatcag caaacttgat ttcctctact atcaatctaa
     6841 atacccgatc caaaaggccc tcttcacctt tttgaatctc agcatttaat gcctgatcgg
     6901 tgacgatgtg taagggctcg tagggaatag ggcaatagaa atcggcttcg ggtaaaatac
     6961 ccgtgcgcga catcaacgta atatttagct tttcacttgc gttatcgcgg tgaaaaacga
     7021 cgtgttgttt atcatcttca atgaacgaac catgctgaat agccactgcc attgccgcat
     7081 ccagtccgct caaggatgtt cccataatac ccacgttaca cgcatcgacc tttgcttcca
     7141 tcaggcctga ccacgggctg ggaaaatacg ttctggttgc ttcttcttca tcaggccaga
     7201 cgtgacccgt ggcaatcacc gctaaatcaa acgtctcgct gggtaaatcc tgatttgtag
     7261 cgagcatgac gccagcattt gtaatttgca gatcggtaac ctggcatgat tcataaacag
     7321 ccactgcaaa tttttgctgt cgtgcctggt ctactaatcg taaaaattga tcgcggaaat
     7381 attcgcccag cagaattcgc ggtaaaaact gacgatcgtg caaggtttct tttttaacgc
     7441 cataacgctg gagatggctg gcttcttgct tttgtagcca ttcgagatac gtacaattaa
     7501 tcggcggtat ttcaatactg gcaatatttg ccagcatcat ttttgagttt tcctcatcac
     7561 tgtatggcat cccgacaccg gcctcgtcag cctgctcgaa gatagaaata gaaagtggag
     7621 tttgttgctg tagaagcgag aataaggtgt agatccccgt aggcccggca cccacaatag
     7681 caattttttt cattgttact tacctgtacg cattctttcg aatttagtgt tatcagataa
     7741 gtttggttga gatttttaga atttgagaaa tttcagacag ggatgaaaag ggcgctaacc
     7801 gcccttgatt aaattctgtc aggcgagttt tgcctgattg atttgactaa gtggctcatt
     7861 gacatcgaaa actttggcga caacttcctc aacgccgaga tttttcagtc gggcagtatg
     7921 tttttccaga taggcaaggg cgcttttttc atcagtgaaa aggtagatcc caccggcttc
     7981 gtggttcttt tcactttctg tccatacctt ccacagaaaa ccaggttcct gattaatcga
     8041 ctcagcaagt ggtttaagct gctcagccat tgcgtcgcca aatgggccat taaaagcaaa
     8101 atgaagttgt aacaaagttg ccatagcgat tctccttctg ataacgcata cgaagacagt
     8161 gtaaatcttt taacgtattg gatacagaga agaaacagag ggaaaaagga aaagaccaga
     8221 ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg acccccacca tgtcaaggtg
     8281 gtgctctaac caactgagct acggacgcag gatggtgcgt tcaattggac tcgaaccaac
     8341 gacccccacc atgtcaaggt ggtgctctaa ccaactgagc tatgaacgca acgttgtagg
     8401 tgacaacggg gacgaatatt agcggcagag tggaaaggtg gcaagaggca aaacgtaatt
     8461 ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa ttggctatat tttgttgatt
     8521 tgcaagggcg atttttattc aggatcgcat ttacatctga tacaacccaa taattcgtgt
     8581 atcagagttt aaacagagtt gtttgccacc atctcaaatc tgtcgtggat aaatcctccg
     8641 tagcctttac acttaggtag caataaaggc acacagatgc agcaaaggaa taaatcatgg
     8701 gatctgatgc gaaaaacttg atgagcgacg ggaatgtgca aattgttaag accggcgagg
     8761 tccttggcgc gacgcaactt actgaaggcg agttgattgt tgaagctggc ggaagagccg
     8821 aaaataccgt ggtcacgggg gctggctggt tgaaagtggc aaccggtggg atcgccaaat
     8881 gcacacagta cggtaacaat ggcacgctat cggtcagcga cggtgccatt gccacagata
     8941 ttgttcagtc cgagggaggc gcaattagtc tctctacgct cgctacggtt aatggccgcc
     9001 atcccgaagg tgaattcagc gttgatcagg gttatgcctg cggtttgttg ctggaaaatg
     9061 gcggtaacct gcgtgtactg gaaggacatc gcgcggaaaa aatcattctc gatcaagagg
     9121 gcggcctgtt ggttaatggg acaacctcag cggtcgtggt agatgaaggt ggtgaattgt
     9181 tggtgtatcc aagtggagaa gccagcaatt gtgagattaa tcagggcggc gtttttatgc
     9241 tggccgggaa agccagtgat acgttgcttg ctggtggcac catgaataat ctcggtggtg
     9301 aagactctga cactattgtt gagaatggat ccatttatcg tctggggacg gatggccttc
     9361 agctttacag ttccggtaag acgcaaaacc tgtcggtgaa tgtggggggg cgggctgaag
     9421 tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg aacagtgatc ctgttgtcac
     9481 ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg cgcaccggtt gaactgaccg
     9541 ggagtgttgc attactggac ggcgcttcaa tgattattgg ttatggcgca gagctgcaac
     9601 aatcaacgat tactgtacag cagggcggtg tgttgattct cgacggcagt acggtaaaag
     9661 gtgacagcgt cactttcatt gttggtaaca tcaatctgaa tggcggaaaa ctgtggctga
     9721 tcactggtgc ggcaacgcat gtgcaactga aagtgaaacg cctgcgcgga gagggagcga
     9781 tttgcctgca aaccagtgcg aaagaaattt cacctgactt catcaatgtg aaaggggaag
     9841 ttaacgggga tatacgcgtt gagataacag atgccagtcg gcaaactctg tgtaacgctc
     9901 tgaaattaca gccagacgaa gacgggattg gcgcaacgct ccagcctgcg taaaaacaca
     9961 ggcgcaaccc gtgaaggttg cgccttgtct ttattagcgg gacgctcgtt gcagaatgat
    10021 ggctgacggc atacgttgca ggaaccgcat acgcagcatc atcataatgg ctgccgatgt
    10081 caggccgata ataaagccta tccagaagcc tgctggcccc ataggttcaa cgaccagatc
    10141 ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc cagtaagctg taaaggtgat
    10201 atagaaaatg gaacgcgtat ctttataacc acgcaaaatc ccactgccaa tcacctggat
    10261 tgagtcagaa atctgatata ccgccgccag caacatcaaa tgcgcagcca gcgttacaac
    10321 ctcgggattg tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg tgaaaatggc
    10381 cgtcagggtt gccatacaga cacccaccat aagcccggtc cgcgcagcgg tttgcgcatc
    10441 cagcgttgag ccctgaccca gacgataacc tacgcggata gttaccgctg ccgccagcga
    10501 cattggaagc acaaacatca gtgaactaaa gttcagggca atctggtgtc ctgcgacatc
    10561 aacaataccg agcggagaca ctaacagagc cacgacggca aacagtgtca cttcaaagaa
    10621 cagtgccagc gcaatcggca aaccgagttg aatcagtcgt ttcataaccg caggctcggg
    10681 cttagcggtg cctttttcgt tacgaatatc gcgcatggag cgggcgcgtt taatgtaaga
    10741 aaccatggca aggaacatga cccaatacac cgccgcagta gccacgccac aaccaacgcc
    10801 accgagctca ggcataccga aatgaccata aataaagata tagttcaccg ggatgttcac
    10861 cagcaggccg ataaagccca ttaccatacc cggcttggtt tttgccagac cttcacattg
    10921 gttacgggca acctggaaga acagatatcc cggcgcgccc cacaacaacg cacgcagata
    10981 acccacggct ttgtccgcca gagccggatc gatgttttcc atgtagcgga taatgtaacc
    11041 tgcattccac agcaccagca taatgagaac ggaaacaaaa cctgccagcc agaaaccttg
    11101 tcgcacctga tgcgcaatgc gctcacgtcg accggaacca tttaattgcg cgataaccgg
    11161 cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga tagaagtacc
    11221 gatagcgacc gccgccatgt cggtggcact atagccgccc gccatcacgg tatcgacaaa
    11281 acccatcgca gtttgggcga tttgcgcgag aatcaccggg attgctaatg ctaataacag
    11341 acgcgcttca ctgatatact tctgcacgtg aacacctttt atttgtagtt atatgagaga
    11401 ctaaaaagcc gcctgaatgg gcagcaaaaa gaagaagcag gggaaattca gtctattgta
    11461 gcgaggattt attaattctc cagtgaaaaa atagccattc ttaccccttc actggtaagg
    11521 gcgttttcca cctgctattg tgctgaacag aatgtcttaa ctgatttcag gagttgtaag
    11581 tatgtttacg gggattgtac agggcaccgc aaaactggtg tcgattgacg agaaaccaaa
    11641 ttttcgtacg catgtggtgg agttacccga ccacatgctg gacggcctgg aaaccggtgc
    11701 ttccgtggcg cataacggtt gctgcctgac cgtgacggaa attaacggca accatgtcag
    11761 ttttgacctg atgaaagaaa cgttacgcat taccaatctt ggcgatttaa aagtggggga
    11821 ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa attggcggac acttaatgtc
    11881 aggtcatatt atgaccaccg ctgaagtggc gaaaatatta acctcggaaa ataatcgcca
    11941 gatctggttt aaagtccagg atagtcagtt gatgaaatat atcctgtaca aaggatttat
    12001 tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg ccaacgcgtt tttgcgtcca
    12061 tttaattccg gaaacactgg aacgcacgac tcttgggaag aaaaaacttg gcgcacgcgt
    12121 caacattgaa atcgatccac aaactcaggc agtggtagat acggtagaac gtgtgctggc
    12181 ggcacgagaa aatgccatga atcaaccagg tacagaagcc tgatctgaac tcgcccccga
    12241 agagggggcg atagaatagc ctttagcgag cgactcgaag gccgttttca acaccgcgtg
    12301 agaacaccac ctgccagagc tgaatatcac gggcgcggaa agcacctgcg caggcattca
    12361 gataataggt aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt tctggccatg
    12421 cggcgaggaa tcgttcatac cacgccatca acgtagtatc gtaatcagca ccgaagttat
    12481 gccagtcttc catcacaaag tggggttcgc tggattgagc aatctggcgt acagagggca
    12541 ggcaaccgtt cggaaaaata tatttattaa tccagggatc aacattcaga tcggtttttt
    12601 tcgaaccgat agtatggagc aggaatatac cttccggttt caaattacga tccaccaccg
    12661 caaaataggt atcgtaattt ttcggtccga cgtgctcgaa catccccaca gaaacaatac
    12721 gatcaaactg gtcgttcagg tcacgataat cttgcagcaa aatggtgaca tccaggcctt
    12781 tacagcgttc ctgagccatt ttttgctgtt cggcagaaat ggtgacgccg accacgctta
    12841 cgtcatagtt agatgccatg tagtgcgcta gtccgcccca gccgcagcca atatccagta
    12901 cgcgcatccc tggttttaac tgcaattttt cacaaatcat tttgagcttc gcctgctggg
    12961 cagattccag attatcggca tctttccagt aagcgcagga atattgcatg aagggatcaa
    13021 gcatgcggct gaacaagtca ttacccaaat cgtaatgctc cttgccgact atccaggcac
    13081 gttttttact ctgcagattg aagagacgag cgccggcaat acgcagcgtg tctttgaaat
    13141 gatgggggag ttggttctcg agacctgcgc gtaagacttt gctaaaaaac atatccagtc
    13201 ggtcacattc ccaccagcca tccatataac tttcgcctaa ccccaaagag ccttcctgca
    13261 gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc cgccggggca gaaccgttaa
    13321 tggctatacc ggcacggcta agtaattcgt tggcgatacg gtaccagttg tcatccggta
    13381 cactgacttc ttctatacac gatgaactca tagtttctcc atcactggtc ggtgatcaga
    13441 accttaaaac agcgtagacg ctttttgggc tttgtgagaa atctcgcaga aaaaaccgcg
    13501 agtctataat ccgacgtaga acagagggaa aggaggaacc cttgccgaaa tgaccatcca
    13561 tagtgagtcg ggaacgatcc tgtccccgta aaaacataat atttatcgta gctttaacaa
    13621 ctcgaaatta gtataggccg ctaatttcaa tcatacaaca aaattttgtt agcagcctaa
    13681 gtataagtat atcgatatag atcagtgtga ttcgctatga gcgacttcgg cattgccatg
    13741 attcgggcag tcaacttctt cacaacgttg catcatgtaa cccagcgcga ccagcactac
    13801 tgttgataac atcacgctgg tggtggtgag caatggcgtg ctgatactaa taagccagga
    13861 aactaccaga cttgcgagga agcacagacc cagttgcaga gtgttctgca acgctgcggc
    13921 gcgaccggtt gcatgtggga agggacgcag cgcctgggcg acaacaatag ggtagatcgc
    13981 gccattggca atcgccatca cacagaatgg gatcaggatt tcgaccagcg acacatggct
    14041 aatgaagccc gcagcccagg tcgcaatgac gctgacagca aacagcacca gcaaccacgg
    14101 taataactgc ttgccttgcc atttctgcag tgcggcgcga cagccataac caccaatcag
    14161 aaacgcgata gtttgcggga cataacttaa accaataact gccgggctgt agcccatttc
    14221 actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa ctggctgaac aggctgcgta
    14281 tatcagcacg ttgccgcgat aggttttaga acgtagcagg tcggtaaagg tcagaccatc
    14341 ctgactattg ttacgggcct tcgtcgtggg tttgagccag aaaataggca gaatcagcac
    14401 cacggtaatg gcaaacaggg tggcgaaaat cgcctgccag gaaaaatgga ccagcagcca
    14461 gcttcctaac aggggagcca gtgccggaga cagacccacc agcggcatga tggtcgcaaa
    14521 aatacggttg actttctgtg aaggataata atctgtcact aatgcttgcc agataaccgc
    14581 cgcggcgcag acccccacag cctgtacaaa acgcaatacc agcagcgtgg cggcgttttc
    14641 tacccacagc atccccagac tacctaacgc aaaaattgtc aggccgatta ataacaccgg
    14701 tttacgacca taacggtcgg agagtggccc ccacagaagc tgggctgcgg caaaaccggc
    14761 aaggaacaga ctaaggctgg cactgacagc agacgcaggc gtttgcaggt cggcctgtat
    14821 ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa ccgagtacgc tcaaacccgc
    14881 cagccagact aaaaatcttt tcccaggttg catgtatatt ctctaaatct ctcaattaaa
    14941 caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa acggtaatat ttgatgactg
    15001 gtttcaaaaa ttttgcaggc agactatgtg gtcagaatat tcactcgaag ttgttgatgc
    15061 ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag ctgcatcgtg ttccttctgc
    15121 ggtcagctat accgtgcgtc agctggaaga gtggctggcg gtaccgctct ttgaacgacg
    15181 gcaccgtgat gtggagctga ccgctgctgg agcgtggttt ctcaaagaag ggcgctctgt
    15241 tgtcaaaaaa atgcagatca cccgccagca atgtcagcag atagcgaacg gctggcgcgg
    15301 tcagttagct atcgcagtgg ataatattgt caggccagaa cgtacacggc agatgatcgt
    15361 tgatttttat cgccattttg atgatgtcga acttcttgtc tttcaggaag tgttcaacgg
    15421 tgtctgggat gcgctttccg acgggcgcgt ggaactggca attggcgcaa cacgcgcgat
    15481 tccggtaggc ggtcgttatg ccttccggga tatggggatg ctaagctgga gttgcgttgt
    15541 tgccagtcac cacccgctgg cgttgatgga tggcccgttc agcgatgata cgttgcgcaa
    15601 ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta cctaaacgta ttacctggtt
    15661 gctggataac caaaaaagac ttgtcgtacc tgactgggaa tcatcggcaa cctgtatctc
    15721 ggcaggatta tgcataggga tggtgccaac acatttcgcc aaaccgtggc ttaatgaggg
    15781 gaagtgggta gcacttgaac tggaaaaccc cttcccggat tcggcatgtt gtctgacatg
    15841 gcagcaaaat gatatgtcgc ctgcgttaac ctggctgctt gagtatctca gcgatagtga
    15901 gacgctgaat aaggagtggc tgcgggagcc ggaagagact cccgcaacgg gtgattaacg
    15961 acgatagtcg cggaacgggc cgtcagccac ggagcggcgt tcaatcaaac gcggatgcac
    16021 ttcaatggac tgcggttctt cacgtttgtt gacgatacga tccaacagca tgttgaacgc
    16081 tgtttcaccc agcgaatctt ttggctgatg gatcgtggtc agcgccggcg taaaatagcg
    16141 cgcgttgcgc acgttatcat aaccgatcag cgaaacatcc tgcgggacgc gcaggcccat
    16201 ttcatcagca gcacaaagtg cgcccattgc catgatatcg ccaccacaga agacggcagt
    16261 agggcgatgc ggctgcgaca ggatttgctg catggcgcga taaccggatt caggttcaaa
    16321 gtcaccctgc acaatccagc tttccggcac cttgatcatc gcttcttcca tcgccttcat
    16381 aaaaccggca aggcggcctg cgccggtgtt acgttccagc gggccgggga tgacgccgat
    16441 ttcgcggtga ccgcgttcaa tcagataacg cccggccatg tagccgcctt cgaacgcgtt
    16501 atcaatgacc gcatcggtga agtcagcttt tgcttcaccc cagtccatca ccaccattgg
    16561 gatatggcga tactcttcca gcatcgccag caacggctct gggtactcag aacacatcac
    16621 cagcagacca tcgacgcgtt tttgcgccat catcgacaga taagcccgct gtttctcaag
    16681 attgttccac gcattaccca gaatcagggt gtaacctttc tggaagcaat ttttttcaac
    16741 tgcttcaatg atctcggcaa aataggccgc ttcgctgctg gtcgccagca aaccgataga
    16801 cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta ggggagtagt gtaattcttt
    16861 aatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg aaacgtgttt tgttgatcac
    16921 gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct acatctttta ttgttgccat
    16981 tccatttcac tccagaccct aacttcagct cctgcaaaat cgcaaggtaa acgtttgcct
    17041 ttacacacct tacgcacaac aaaagtgttg cggtacgccg gaaaaacgac acggagcgtc
    17101 aggaaggggt caatggccag tacgcaaata gtgtaagcgt ggaattttgg ccgatcttga
    17161 cgaaaagggg aagtggtaaa aacacttatc attataaatg acgggagatt ttttcatcac
    17221 agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag aaaacttgat
    17281 gagcaccgac cttaaatttt cactggtaac aacggttatc gtcctcggtt tgatcgtagc
    17341 cgtgggtttg actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt ctccctcgca
    17401 tctccttcct gtttgttagc aaaatggcaa aagtgttttc tctttttgtt attttcattt
    17461 ttttgtgatt gctgtcatgc tttcgccact ttaatgaata tcgcgtaatg acgcgaagag
    17521 gtggagataa ggcatgaaaa ttaactatcc gttgctggcg ctggcgattg gcgcgtttgg
    17581 tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc gcggtgtgga
    17641 tgtctcgatt cccgctgccg gaatgttaat cagtgcctat gcagttggcg taatggtggg
    17701 cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt tgattttcct
    17761 gatggcaatt ttcacgctag gcaacgttct ttccgccatc gcgccggatt atatgaccct
    17821 gatgctttca cgtattttga ccagcctgaa tcacggagca ttttttggtt tgggttcagt
    17881 cgtggccgca agcgtggtgc caaaacataa acaggccagc gcagttgcca ctatgtttat
    17941 ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg gtgaaaccat
    18001 cggctggcgg atgtcatttc tggcaacggc ggggctggga gtgatttcaa tggtaagtct
    18061 gttcttctca ttacctaaag gtggtacagg ggcacgacct gaagtgaaaa aagagctggc
    18121 ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg gagctggtgc
    18181 aatgtttacc ctctacacct atatctctcc ggtactgcaa agtattaccc acgcaacacc
    18241 ggtgttcgtc acggcaatgc tggtgctgat tggtgtcgga ttctctatcg gtaactatct
    18301 cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt tgttgctgct
    18361 gatggtgatt atgctggcaa tcccgttcct ggcccgcaat gagttcggcg cagctattag
    18421 catggtggtg tggggcgcag caacctttgc ggttgtacct ccgttacaga tgcgcgtgat
    18481 gcatgtcgcc agtgaagcgc caggtctgtc ttcatcagtc aatattggtg cctttaatct
    18541 tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc tgggatacag
    18601 ctttgtgccg gtgacggggg cgattgtcgc gggactggca ttattgctgg tgtttatgtc
    18661 agccagaaaa caacctgaaa cagtttgcgt tgctaacagc taattaaaca aaaacgcaga
    18721 aggataacct tctgcgttac agttgctgac aacgtgcgat ttatcttgcc gaatgcgctg
    18781 ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat caggcaatgc tgcatttgcc
    18841 atcagttatt atgcagcgag atttttcgct acgaattccc agttcaccag cgcccagaag
    18901 tgctccagat agccaggacg tgcattgcga tagtcgatgt aataagcgtg ttcccagaca
    18961 tcaacggtca gcagcggagt cgcatcggtg gtcagcggag tacccgcgtt agaggttgaa
    19021 acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga accaaagttt
    19081 ttgatcgctg catcagtaaa ctgcgctttg aaatcggcaa agctgccaaa agatgcggcg
    19141 atagcttcag cgacttttcc agtcggttcg ccaccggcgt tcggtgccag gcagttccag
    19201 tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc agagctgcga
    19261 ataatctctt ccagtgattt accttcaaac gcggtacctt taatcaggtt gttcaggtta
    19321 gtgacataag tctgatgatg cttgccgtag tgatactcga tagtttccgc agaaatgtgc
    19381 ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat tgctactctc
    19441 ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata gggtagcaaa
    19501 ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg taaagtttta
    19561 gcgaagtgtt tttggggtca ttacccgacg agcgccaaca tagtggcgct gccagtagtc
    19621 ttcactgaga gaagtgattt gaatttcctg acctgtgcgc ggtgactgaa taaatttgcc
    19681 gttgccgaca tacacgccga catgatcggc tgtaccgcgt ccctgagtac ggaaaaagac
    19741 caggtcgccg tttttcagct cactacgttc gataggtcct gcatcacgca ggtgatacat
    19801 ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt aataaaccag
    19861 gccgctgcaa tcaaaaccgg tacgcggtga gctgccgccc caacgatatg gcttaccaat
    19921 ttgctgcatc agtttattca ttgccacttt cgtggctttt tgcaccttcg ctttgtgcgc
    19981 gtcggcaagg gttcctgacg cggcattttt gactttcaca caatgcgatt tatagccctt
    20041 acgtttggta cacttttcgg taaagctggc tgaggccgtt ttattgacgg cgtgtttgct
    20101 gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacagaag aagcggtttt
    20161 gcttttggtc atacttttac tggcggcttt tttcgctgtt ttctggctgg ctttctgggt
    20221 ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat gagtggtagc
    20281 agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca gggtggtaaa
    20341 aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc gtccctctgt
    20401 taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct aatgcataga
    20461 agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg agcgcaaaaa
    20521 aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccgcg catcagagaa
    20581 gaaacgaata tttgaaatgc aacaaataga gcaaagggat aaaatagcaa aagcgtgatg
    20641 aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact gccgtaaaga
    20701 agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc agattgctga
    20761 aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg gtttctctgc
    20821 ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg atattctgca
    20881 gaatccggac attcgtgcgg aactgccgaa atatgctaac tggccgacct tcccgcaact
    20941 gtgggttgac ggcgagctgg tcggcggttg tgatatcgtg atcgaaatgt atcagcgtgg
    21001 cgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag agccggacgc
    21061 ggaataatcc gtcctgcaat actgacggat gcgaacgggc atccgtcagt cctttatccg
    21121 gtgcgccgta aaacctcgtc cttcagggcg gggatataag gcgcggtttt ccacctgacc
    21181 aggtgtttgc tgttgttcaa tgtactggcg gatgacggat attggcgcac cttcgcagct
    21241 actggcaaaa tagccaggac tccacaaaac gcctttgtag taatacctga ctgcaatatc
    21301 tggtcgatct cgtcgcagta acctattcga tatgcctttg aggctgttta ccagactgga
    21361 tatcgccagt ttgggaggat agttaattaa caaatggacg tgatctggtt cgccatccat
    21421 ttcaaccagt tcagctgcaa aatcagcaca tacatttgaa aagtaagtgc gtagtttttc
    21481 tgtcgtgtca tggtcaaaaa tctggcgtcg gtatttggtg acaaagacca ggtgaacatg
    21541 catcaggaaa acacaatgcc ttccacgccg gatatcggtt tcttttttca cagaccaaag
    21601 tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac ccggtggtca
    21661 acaggagcgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct
    21721 ggcgcttcag aatgagaatc atgaggccgg taataaatac atcccttacg ggaaaatggc
    21781 ttcctggctg gttgagtgga aaaacgccac tgaaacgcaa tggcttaaag attcgccctc
    21841 acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact tcttccagaa
    21901 gcgggctgct tttccccgat tcaaaaagcg gggacagaat gctgcattcc gctacccgca
    21961 ggacgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaacttg gctggatgcg
    22021 ctaccggaat agccgtcagg tcacgggagt tgtgaaaaat gtcactgtca gccagtcctg
    22081 cggtacgtgg tacatcagta ttcagacaga aagtgaagta tcaactccgg ttcacccttc
    22141 agcatcaatg atcgggctgg atgctggcgt ggctaaactc gccacgctgt cagatggcac
    22201 agtctttgag cctgtaaaca gttttcaaaa aaaccagaag acgctggcga gacttcagcg
    22261 acagttaagc cggaaggtca aattcagcaa caactggcag aagcagaaac gcaaaataca
    22321 gcgactgcat tcctgtatcg caaatatccg cagggactac cttcacaaag tcacaacgac
    22381 cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg aaggtcagca acatgtcaaa
    22441 gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc cgggcaaaat caggtttaaa
    22501 ccgttcgata ctggatcagg gctggtatga aatgcgccgc cagcttgagt acaagcagct
    22561 ctgg
//
LOCUS       DAJTRM010000064        22402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRM010000064 DAJTRM010000000
VERSION     DAJTRM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.064"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            72..2672
                     /locus_tag="OO322_002625"
     CDS             72..2672
                     /locus_tag="OO322_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5907449.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2663..3646
                     /locus_tag="OO322_002626"
     CDS             2663..3646
                     /locus_tag="OO322_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5907450.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3744..4284
                     /locus_tag="OO322_002627"
                     /pseudo
     CDS             3744..4284
                     /locus_tag="OO322_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4292..4807
                     /locus_tag="OO322_002628"
     CDS             4292..4807
                     /locus_tag="OO322_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5907451.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4773..5510
                     /locus_tag="OO322_002629"
     CDS             4773..5510
                     /locus_tag="OO322_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5907452.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5621..6631
                     /gene="pyrD"
                     /locus_tag="OO322_002630"
     CDS             5621..6631
                     /gene="pyrD"
                     /locus_tag="OO322_002630"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5907453.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6805..7347
                     /gene="zapC"
                     /locus_tag="OO322_002631"
     CDS             6805..7347
                     /gene="zapC"
                     /locus_tag="OO322_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5907454.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7344..8453)
                     /gene="ycbX"
                     /locus_tag="OO322_002632"
     CDS             complement(7344..8453)
                     /gene="ycbX"
                     /locus_tag="OO322_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5907455.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8697..10805
                     /gene="rlmKL"
                     /locus_tag="OO322_002633"
     CDS             8697..10805
                     /gene="rlmKL"
                     /locus_tag="OO322_002633"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5907456.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10817..12724
                     /locus_tag="OO322_002634"
     CDS             10817..12724
                     /locus_tag="OO322_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5907457.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12854..14107
                     /gene="pqiA"
                     /locus_tag="OO322_002635"
     CDS             12854..14107
                     /gene="pqiA"
                     /locus_tag="OO322_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5907458.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14112..15752
                     /gene="pqiB"
                     /locus_tag="OO322_002636"
     CDS             14112..15752
                     /gene="pqiB"
                     /locus_tag="OO322_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5907459.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15749..16312
                     /gene="pqiC"
                     /locus_tag="OO322_002637"
     CDS             15749..16312
                     /gene="pqiC"
                     /locus_tag="OO322_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5907460.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16568..16735
                     /gene="rmf"
                     /locus_tag="OO322_002638"
     CDS             16568..16735
                     /gene="rmf"
                     /locus_tag="OO322_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5907461.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16805..17323)
                     /gene="fabA"
                     /locus_tag="OO322_002639"
     CDS             complement(16805..17323)
                     /gene="fabA"
                     /locus_tag="OO322_002639"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5907462.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17392..19152)
                     /locus_tag="OO322_002640"
     CDS             complement(17392..19152)
                     /locus_tag="OO322_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5907463.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19337..19789
                     /gene="matP"
                     /locus_tag="OO322_002641"
     CDS             19337..19789
                     /gene="matP"
                     /locus_tag="OO322_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5907464.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19865..20911)
                     /gene="ompA"
                     /locus_tag="OO322_002642"
     CDS             complement(19865..20911)
                     /gene="ompA"
                     /locus_tag="OO322_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5907465.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21267..21776)
                     /gene="sulA"
                     /locus_tag="OO322_002643"
     CDS             complement(21267..21776)
                     /gene="sulA"
                     /locus_tag="OO322_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5907466.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21995..>22402
                     /gene="sxy"
                     /locus_tag="OO322_002644"
     CDS             21995..>22402
                     /gene="sxy"
                     /locus_tag="OO322_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5907467.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5717 a         5255 c         5872 g         5558 t
ORIGIN      
        1 tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc gtcttcagtt
       61 aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct ggtggagtgc
      121 catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca caagctgaaa
      181 cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat ttgtctcgtt
      241 tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt ctgaatcaga
      301 caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag gggattgccg
      361 cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg tttccggctt
      421 ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg gatgccagcg
      481 taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc gccatcaaaa
      541 gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac gcgctattac
      601 tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt gattctggcg
      661 acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga ttacgcaaca
      721 attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc agcagctatt
      781 tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat tatactgcag
      841 gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat gacaacatgc
      901 tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa agcaatgccc
      961 agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg cctggtgctt
     1021 ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt gaaatcaaag
     1081 aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca ttactccagc
     1141 gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat agtaatgaac
     1201 agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg ggaacgacat
     1261 ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt ctgggattta
     1321 accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt acgctggcag
     1381 accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg ctcaaccaat
     1441 tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc tacacccttt
     1501 ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat gatgaagata
     1561 cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa ctgcaggtca
     1621 atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc cagcaaactt
     1681 actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg caaattaaag
     1741 atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct gatgctgatc
     1801 aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga agtaatgata
     1861 gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc gataacgaag
     1921 ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt aacctgagct
     1981 acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc gccagcatgg
     2041 actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat aacggcagtc
     2101 aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag ggcattaccc
     2161 tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca gagaatactc
     2221 gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt gtcccctatg
     2281 ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa cgtaacgtgg
     2341 atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt ctggcggagt
     2401 tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt ataccgctgc
     2461 gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt attgatgatg
     2521 atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta cgctggggcg
     2581 aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa attaactctg
     2641 cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg tgttattact
     2701 acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg cttatatcgg
     2761 gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg ttaaaactct
     2821 ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt ggaacgaatc
     2881 gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg gccgatgggc
     2941 attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa ttaatgacta
     3001 tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg ttcctttcga
     3061 aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg gtcaacgttt
     3121 aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat tgcagccgtt
     3181 cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat gctacaacat
     3241 acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt acctgtctgg
     3301 tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg aggttgatct
     3361 gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg gggccagaac
     3421 ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag ttaacgtcaa
     3481 tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga cgtcacgtcc
     3541 tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg ctggtgggac
     3601 actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt ctatccagtc
     3661 agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata
     3721 aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc gttattttgt
     3781 tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc agtatgcata
     3841 tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata
     3901 ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta agcactgctg
     3961 ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg gacgtccttt
     4021 ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac acaggcggaa
     4081 gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca gcaagaaatc
     4141 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
     4201 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
     4261 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
     4321 cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg tcaacattag
     4381 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
     4441 gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg agaaatcttt
     4501 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca ccttttcagg
     4561 aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg atgcagccag
     4621 tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
     4681 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
     4741 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
     4801 tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg ctgctggtgg
     4861 cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
     4921 gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt attaacagca
     4981 aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca gcactattac
     5041 tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg cgcttgctga
     5101 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
     5161 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
     5221 aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat caacaattac
     5281 gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac tacattaacc
     5341 tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg ccgcctaaaa
     5401 gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc tggcggacga
     5461 taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga aacaggttct
     5521 gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa ctcgcacata
     5581 atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc ccttcgttcg
     5641 taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc agcaattacg
     5701 ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg cgaaacctgt
     5761 taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc ttgataaaga
     5821 cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga tcggtaccgt
     5881 cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg tagatgccga
     5941 aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg tagagaacgt
     6001 aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata aagatacgcc
     6061 agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg cctatgcggg
     6121 atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc tgcaatatgg
     6181 tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt tgcaagcgat
     6241 gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg aagacgaatt
     6301 gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg caaccaatac
     6361 cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg gtggcttaag
     6421 tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac tagaattaaa
     6481 cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg cgcgtgaaaa
     6541 gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta aaggtccgcc
     6601 gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc tttataagat
     6661 aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc ttatttagac
     6721 attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt gttgctgcgg
     6781 ttttataggg taggggagag gcagatgcga attaaaccag acgataactg gcgttggtat
     6841 tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct atttcgctca
     6901 cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt ttgtgttgat
     6961 gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt atctaaagac
     7021 caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa accgcaaatg
     7081 ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc gggcgatgcg
     7141 gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt tgaatctggc
     7201 gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg tcgcgcgatg
     7261 cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt gaatgttgac
     7321 agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt cggtacacag
     7381 ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg tgtgacttcg
     7441 ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga ataagggata
     7501 cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc acgaaatgcc
     7561 tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt ggcggtatca
     7621 tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc cacctcatcg
     7681 ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc gacatcgccg
     7741 ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc cggatgtttt
     7801 tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt aaccacatca
     7861 aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc tgacgcgccg
     7921 gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca acgttgttgg
     7981 agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc aaatgacaga
     8041 ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc
     8101 acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc aattcgcgcg
     8161 gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga
     8221 acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac aggtgaagga
     8281 gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc aggttccgtg
     8341 atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg tgtaagacca
     8401 atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc cacggtgcgg
     8461 tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag ctataatgcg
     8521 caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt ggccctgggt
     8581 tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc tccatgtcca
     8641 gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt gacgatatga
     8701 attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact gaactggaaa
     8761 atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag ggcgacacac
     8821 ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg ttgccgctgg
     8881 gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg atcaactgga
     8941 cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg aatgacacca
     9001 tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat gctttcacac
     9061 ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct
     9121 ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt ttacatctgc
     9181 gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca gccgctattg
     9241 tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt ggttccggta
     9301 cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg caccgtgggc
     9361 gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa gtgaaagcgg
     9421 aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt tacggttcgg
     9481 acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg gggattggtg
     9541 aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg ccgaaagggc
     9601 cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc gaaccggcgc
     9661 tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt ggctggaatc
     9721 tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt gcagacaaac
     9781 agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac gttgccgaaa
     9841 gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc ctgcgtaaga
     9901 acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac cgcctgtatg
     9961 acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc
    10021 aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt ctgttcgata
    10081 ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc
    10141 gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc gagtttcttg
    10201 aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc gatactggtc
    10261 tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa ggcaaagatt
    10321 tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta ggcggtgcac
    10381 gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa cgcaacctgc
    10441 gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc
    10501 tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc ttctctaact
    10561 caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg atgaaagatt
    10621 tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa cgtggcttcc
    10681 gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt acgcaaaaaa
    10741 cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg attaccgcag
    10801 cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt cgttcagcga
    10861 cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg tttgtctggt
    10921 gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct
    10981 ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc aacaggatcc
    11041 gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg aagaacaagc
    11101 ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc cgagcgagaa
    11161 aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc tgtggcagct
    11221 ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg ttgcgctgtc
    11281 gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg tgagcaatcc
    11341 gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca tcgactggct
    11401 ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg accgttcgtt
    11461 tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg tgacctatcc
    11521 agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg aagaactaca
    11581 aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc aggggatcaa
    11641 agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc gtcgcgaacg
    11701 tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg ccagccgctc
    11761 cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta agcaactggt
    11821 gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg gtccgaatgg
    11881 gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg acagcgggcg
    11941 tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg cggaactgga
    12001 tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga tggttaatgg
    12061 caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga aacgggcgat
    12121 gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt
    12181 cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg atgtcgaaac
    12241 gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc tggttagcca
    12301 cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag gcggcggtaa
    12361 aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac aatatgtggc
    12421 actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag cagaaactgt
    12481 aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc agctaccgca
    12541 attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg cggatgcttc
    12601 cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg ctgctgcaga
    12661 gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg
    12721 ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat tgcctgatga
    12781 actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa tagcgcatca
    12841 ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt gctcgcagtg
    12901 tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat gtccccggtg
    12961 tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg cctatgcgtt
    13021 ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc
    13081 aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt ctgaggacta
    13141 cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat
    13201 aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag agcaactggc
    13261 acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg cgggtgtgct
    13321 ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca gctttctccc
    13381 ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt
    13441 atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag gcgtcacggg
    13501 gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg ctgatgaacc
    13561 cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc tgcagtggac
    13621 actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt tgcccatcat
    13681 ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt
    13741 atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc
    13801 aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc atggcaagcg
    13861 cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc gctggtcgat
    13921 gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag gtttaatgag
    13981 tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga caatgttttc
    14041 tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga
    14101 gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg aagaactggt
    14161 ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt cttttttatc
    14221 attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag
    14281 gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac
    14341 tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt atggaaaaat
    14401 tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc gaagggatta
    14461 gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc gcgaaaggca
    14521 gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct gatgcgaaag
    14581 gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga gatccggtgc
    14641 tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg caaaaacgca
    14701 atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc agcaatgttc
    14761 gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg cgtgtggaga
    14821 tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg gaaggtctgg
    14881 atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat cagaagagca
    14941 ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa gattcggtac
    15001 gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt accgtaagca
    15061 aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac cgtattccgg
    15121 tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat gcggatgttg
    15181 ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc
    15241 tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct gcaataaccg
    15301 gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc ttggcgcaaa
    15361 tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg aatccgatga
    15421 ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga
    15481 cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg ccgacggata
    15541 tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct ggctccgcag
    15601 cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga gaactgcaac
    15661 cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg aaggacaaaa
    15721 aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact
    15781 gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt tacctgtggt
    15841 gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg tagagcaggt
    15901 cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg atgtgaagta
    15961 tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc gcaacaccct
    16021 ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc ctctgggaag
    16081 cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt
    16141 cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac gtccgttccg
    16201 tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc tggccggtgt
    16261 ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt aattataaag
    16321 atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt ttctaacgtg
    16381 cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg tagctcacaa
    16441 atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg tattgcttaa
    16501 ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag tatgagggaa
    16561 acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac gtggttatca
    16621 ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga gtcaaaggtc
    16681 acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg cctgattctg
    16741 tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact ctgtctgatg
    16801 ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg gcggtataga
    16861 tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga cgacgattaa
    16921 caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt acctgaccag
    16981 tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc agccagccga
    17041 ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc ataaccggat
    17101 cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt tctgcttcaa
    17161 catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg tctatcatca
    17221 gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg tgaccagagg
    17281 caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct gtaagcctta
    17341 ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg atcagttcgg
    17401 aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga
    17461 tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt cgccatccca
    17521 caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc agatggtgaa
    17581 tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc gaacgttagc
    17641 agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa tagcaaagaa
    17701 gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact gatcgactga
    17761 acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta tcagggcgca
    17821 gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct caaatgtcag
    17881 cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga acgcctgcat
    17941 gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt
    18001 gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg
    18061 tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc
    18121 ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag cgaggaaacc
    18181 ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc
    18241 atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc taagcggaag
    18301 tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc aagcatccgc
    18361 tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac accactggct
    18421 tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa tagcctgctc
    18481 tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc ctaccagaat
    18541 gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg actcatcaaa
    18601 cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc acagcagagg
    18661 ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct gatgcaccag
    18721 accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga gttgctccgc
    18781 ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat ctgcactcac
    18841 tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca ccagttggcc
    18901 tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact cagactcttc
    18961 cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact gctccagcgc
    19021 aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg
    19081 ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc atgcaagttt
    19141 cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc
    19201 ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt aaagctcaca
    19261 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    19321 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    19381 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca tagaagccag
    19441 tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg tgctggtaaa
    19501 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc agaccattcg
    19561 ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    19621 tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg gtaaaacgct
    19681 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    19741 aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    19801 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    19861 taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    19921 gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    19981 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    20041 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    20101 aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg tctttcggat
    20161 ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    20221 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    20281 ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa cggtaggaaa
    20341 cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg tcaccgatgt
    20401 tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg tactcaacac
    20461 cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg ccaccaggta
    20521 cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag atgtccagat
    20581 cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct ttgtatgcgc
    20641 cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc atttcaaaac
    20701 caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg ttttcgtggg
    20761 tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc agtttagcac
    20821 cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg
    20881 ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca tccaaaatac
    20941 gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga tctgctcaat
    21001 attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta aagtctacaa
    21061 cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa gtcttgtata
    21121 aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg aaaccgcttc
    21181 agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa
    21241 agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg
    21301 gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa ccccatagcg
    21361 ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgtc
    21421 aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccact
    21481 gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc
    21541 tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc
    21601 tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg
    21661 cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt
    21721 ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc
    21781 aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca acaaagatca
    21841 accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta
    21901 atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat
    21961 cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat
    22021 ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg
    22081 cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta
    22141 tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac
    22201 atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg
    22261 gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga
    22321 gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct
    22381 ggaagcgatt ctcggggagg tg
//
LOCUS       DAJTRM010000065        22282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRM010000065 DAJTRM010000000
VERSION     DAJTRM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.065"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1029)
                     /locus_tag="OO322_002645"
     CDS             complement(<1..1029)
                     /locus_tag="OO322_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907468.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAK"
     gene            complement(1131..2111)
                     /locus_tag="OO322_002646"
     CDS             complement(1131..2111)
                     /locus_tag="OO322_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5907469.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2119..2829)
                     /locus_tag="OO322_002647"
     CDS             complement(2119..2829)
                     /locus_tag="OO322_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5907470.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3046..4869)
                     /gene="typA"
                     /locus_tag="OO322_002648"
     CDS             complement(3046..4869)
                     /gene="typA"
                     /locus_tag="OO322_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5907471.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5242..6651
                     /gene="glnA"
                     /locus_tag="OO322_002649"
     CDS             5242..6651
                     /gene="glnA"
                     /locus_tag="OO322_002649"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5907472.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6824..7873
                     /gene="glnL"
                     /locus_tag="OO322_002650"
     CDS             6824..7873
                     /gene="glnL"
                     /locus_tag="OO322_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5907473.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7885..9294
                     /gene="glnG"
                     /locus_tag="OO322_002651"
     CDS             7885..9294
                     /gene="glnG"
                     /locus_tag="OO322_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5907474.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9406..9516
                     /locus_tag="OO322_002652"
     CDS             9406..9516
                     /locus_tag="OO322_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5907475.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9745..11118)
                     /gene="hemN"
                     /locus_tag="OO322_002653"
     CDS             complement(9745..11118)
                     /gene="hemN"
                     /locus_tag="OO322_002653"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5907476.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11307..11816)
                     /gene="yihI"
                     /locus_tag="OO322_002654"
     CDS             complement(11307..11816)
                     /gene="yihI"
                     /locus_tag="OO322_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5907477.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12398..13030
                     /locus_tag="OO322_002655"
     CDS             12398..13030
                     /locus_tag="OO322_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5907478.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13412..16198)
                     /gene="polA"
                     /locus_tag="OO322_002656"
     CDS             complement(13412..16198)
                     /gene="polA"
                     /locus_tag="OO322_002656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5907479.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16562..17494
                     /locus_tag="OO322_002657"
     CDS             16562..17494
                     /locus_tag="OO322_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5907480.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17535..18965)
                     /locus_tag="OO322_002658"
     CDS             complement(17535..18965)
                     /locus_tag="OO322_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5907481.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYSPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19120..19746)
                     /gene="dsbA"
                     /locus_tag="OO322_002659"
     CDS             complement(19120..19746)
                     /gene="dsbA"
                     /locus_tag="OO322_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5907482.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19763..20749)
                     /gene="srkA"
                     /locus_tag="OO322_002660"
     CDS             complement(19763..20749)
                     /gene="srkA"
                     /locus_tag="OO322_002660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5907483.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20826..21095)
                     /locus_tag="OO322_002661"
     CDS             complement(20826..21095)
                     /locus_tag="OO322_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5907484.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21165..21749
                     /gene="mobA"
                     /locus_tag="OO322_002662"
     CDS             21165..21749
                     /gene="mobA"
                     /locus_tag="OO322_002662"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5907485.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21731..22258
                     /gene="mobB"
                     /locus_tag="OO322_002663"
     CDS             21731..22258
                     /gene="mobB"
                     /locus_tag="OO322_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5907486.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5514 a         5583 c         5334 g         5851 t
ORIGIN      
        1 ttttgcttcg ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa
       61 gcccagcgta catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa
      121 cagtacgagc gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt
      181 caggatctta tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg
      241 gttaatgatg ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa
      301 tggaatgaag aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat
      361 aagccagatg gttttgtctc tcagcactga ggtcatactg gtgttttgcg atgctccgtc
      421 ttcttttttc acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaataat
      481 aatgcctacg gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa
      541 gccactgcca aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat
      601 gccacgccct gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg
      661 acttaccgac ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc
      721 ccagacaaag aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc
      781 tgtcgcaatg agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat
      841 agacagaaaa aagcccactg tggtgacaaa tgagtttacc gacatagcat taccaatttg
      901 accattggtc aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg
      961 taatttataa attgtaccgc cacacagtgt taatagacta aataacgccc atttcttttt
     1021 cgtgagcata acatccccta agcgattttt aaaatcactg ttttaaaggc aataagatac
     1081 cgaccattca tatggaatgg ttttattaac tgcgtatgag ttaaaactta ttagtggttc
     1141 agaagatggt ttgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga
     1201 ttaattgcat cttctttttc tttacgtaac cgcgcatcca gtaaatgatt ttctggtagg
     1261 ggtgtttcac tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg
     1321 aacgccgacg catagtaatc aacctcttct tccaggccat atgccgtcac ctgcggctca
     1381 tcatcgccaa ggcagataag gtgcgttaat aacgctttga atggcatatc accctgacct
     1441 gaaatacagg ctttatgacc caggccaccc tgttctttaa cgatcaaggc atctttaata
     1501 tggacctggg taatatgcgg tgccatcgtt tttaaggcgt cgatgggatg ctcatttgca
     1561 ttgatcatgt tagcaaaatc aaacagtaag gaaagagact ccatctcact ttctttgacc
     1621 agcgacacca gctcatgact ttttaaatct tcatgctgct caatcgtaaa agtcaggccg
     1681 ctgtcctgat acgtttcccg tacataggca atgtcgttag cgataatcga taatacgtcg
     1741 cgcaaattac cttcataaca tggataaaaa cgtacggacg ttgccccagt tttcaacgcg
     1801 atggcgacgg cttcgtcaat agatgcctta tctgaagcgc tggtttcaat atgaatatca
     1861 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
     1921 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactg
     1981 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
     2041 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
     2101 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
     2161 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
     2221 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
     2281 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
     2341 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
     2401 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
     2461 agttgatggc gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt
     2521 tttctcggta aacccccaag aaggctctcg cccttgctca attgcggccc gctgaaagct
     2581 cgccgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
     2641 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
     2701 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
     2761 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
     2821 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
     2881 aaaagaaagc aattaacggt gtatgacttt gcagataatt gtgacaaagc tctctattga
     2941 cgtaatcaca gacttataag ggagatactc gcattggtgt gcgctcacga caggtaaaaa
     3001 aaaacctgcc atcgctggca ggttttttat gactaaaaaa cgaaattaat cgtctttcgg
     3061 tgcgcggttg gcgcggcgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga
     3121 ggtcggagtc acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt
     3181 catgcggata ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt
     3241 cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat
     3301 aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcag
     3361 accgaacagc gcgaacgcga cagctttacc ctgaccgtta gagatcagta caccgttctg
     3421 acgctgaccc acttcgcccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag
     3481 accagtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg
     3541 gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc
     3601 tttacgttcg cccagcgcct gcatcacaga accctgatgc tgttcttcaa cgtccagcgt
     3661 cacgttttca tacggctctt gtttacgacc gtctatttca cggaagatta ctttcggacg
     3721 ggataccgcc agttcgaaac cttcacgacg catgttttcg ataagaacag agaggtgcag
     3781 ttcaccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc
     3841 aacgttgtgt accagttctt tgttcagacg atcgaggatc tgacgagacg ttacgaactt
     3901 accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc
     3961 atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat
     4021 gttcagttcg cccaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc
     4081 gatacgttcc agaccgaggt ggcccagcac tttaccgact ttcgcgttac gggttttgcc
     4141 ttcgctatcg atgatagtaa cctgctggtt cggcttcact ttaccacgct tgatgcggcc
     4201 aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg
     4261 accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctgat acagcggggt
     4321 catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta
     4381 aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac
     4441 ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat
     4501 cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc
     4561 gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc
     4621 accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt
     4681 gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc
     4741 catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt
     4801 gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt
     4861 ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg
     4921 cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga
     4981 tcgctttcac ggagcataaa aagggttatc caaaggtcat tgcaccaata tggtgcttaa
     5041 tgtttccatt gaagcactat attggtgcaa cattcacatc atggtgcagc ccttttgcac
     5101 gatgttaagc atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt
     5161 atctttttta cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgacaatga
     5221 ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac
     5281 gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca acacgtcact
     5341 atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc
     5401 tcgattggcg gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc
     5461 actgcagtga ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt
     5521 gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa
     5581 gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc
     5641 ttcctgttcg atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac
     5701 gatatcgaag gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt
     5761 ccggcagtga aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt
     5821 tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa
     5881 gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct
     5941 gacgaaattc agatctacaa atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc
     6001 gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg
     6061 tctctgtcta aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag
     6121 caggcgctgt actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca
     6181 aacccgacca ccaactctta taagcgtctg gtcccgggct acgaagcacc ggtaatgctg
     6241 gcttactctg cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa
     6301 gcacgtcgta tcgaagtacg tttcccagat ccggcagcta acccgtacct gtgctttgct
     6361 gccctgctga tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg
     6421 gacaaaaacc tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc
     6481 tctctggaag aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc
     6541 gtgttcacgg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc
     6601 gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag
     6661 ttgtactacc cggcgcacaa cgccgggatt tagttgccgt ggaaactttc agcccatctc
     6721 tgcatgggct tttttctccg tcaattctct gatgcttcgc gctttttatc cgtaaaaagc
     6781 tataatgcac taaaatggtg caacctgttc aggagactgc tttatggcaa caggcacgca
     6841 gcccgatgct gggcagatcc tcaactcgct gattaacagt attttgttaa ttgatgacaa
     6901 tctggcgatc cattacgcca accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa
     6961 attgtttggt acgccgttac cggaactgtt gagctacttc tcattaaata tcgagctgat
     7021 gcaagaaagt ctggaggcgg ggcaaggctt taccgataac gaagtgacgc tggtcatcga
     7081 tggtcgctcg catatccttt ctgtgacggc ccagcgtatg ccggacggca tgatcctgct
     7141 ggagatggca ccgatggata accagcgccg cttaagccag gaacaactac aacatgctca
     7201 acaagtagct gcccgtgatt tagtgcgcgg cctggcacat gagattaaaa atccgcttgg
     7261 cggtttacgt ggcgcggcgc agttactcag caaagcgtta cctgacccat cactactcga
     7321 atataccaaa gtgattatcg aacaggcgga ccggctgcga aatttggtcg accgtctgtt
     7381 ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt
     7441 ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg attacgatcc
     7501 cagcctgccg gaactggcgc acgacccgga tcaaattgaa caggtcttgc tgaatattgt
     7561 gcgcaatgcg ctacaggcgc tggggccgga aggcggtgaa atcattctgc gtacccgcac
     7621 cgcgtttcaa ctgaccttac acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga
     7681 agataacggg ccgggcattc cgcctcattt gcaggatacg ctgttttacc cgatggtcag
     7741 cggccgcgaa ggtggcaccg ggcttggctt atccatcgcc cgtaatttga ttgatcagca
     7801 ttcaggcaaa attgaattta ccagttggcc aggtcatacc gagttctcgg tttacctgcc
     7861 tatcaggaaa taaaggtgac gtttatgcaa cgagggatag tctgggtagt cgatgacgat
     7921 agttccatcc gttgggtgct tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca
     7981 tttgagaacg gcgcggaagt actggaggcg ctggcgagca aaacgccgga tgtgctgctt
     8041 tcagatatcc gtatgccggg aatggacggg ctggcgctgc tcaagcagat taaacagcgc
     8101 catccaatgc ttccggtcat cattatgacc gcacattccg atctggatgc tgccgtcagc
     8161 gcctatcaac aaggggcgtt tgattatctg cccaaaccgt ttgatatcga cgaagcagtg
     8221 gcgctggttg agcgcgctat cagtcattac caggaacagc agcagccgcg taatattcag
     8281 cttaacggcc caacgaccga tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt
     8341 attatcggtc ggctttcgcg ttcttctatt agcgtgctga ttaacggcga atccggcacc
     8401 ggtaaagaac tggtcgctca tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt
     8461 atcgcgctga atatggcagc tatcccaaaa gatttgatcg aatcagaact gtttggccac
     8521 gagaaaggcg cgtttactgg cgcgaatacc attcgtcagg ggcgttttga acaggccgat
     8581 ggcggcacgt tatttctcga cgaaattggt gatatgccgc tggatgtgca gacgcgtttg
     8641 ctgcgcgtgc tggcagacgg tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg
     8701 gatgtgcgga ttatcgctgc cactcaccag aatcttgaac agcgggtgca ggaaggtaag
     8761 ttccgtgagg atctgttcca ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc
     8821 gaacgtcggg aagatattcc ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa
     8881 ctgggcgtag aagcgaagtt gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg
     8941 tggccaggca acgtgcgcca gctggaaaac acctgccgct ggctaacggt gatggccgcc
     9001 gggcaggaag tgttgattca ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag
     9061 agtacttcgc aaatgcaacc ggacagttgg gcgacacttt tagcgcagtg ggcagacaga
     9121 gcgctgcgtt ccggtcatca aaatctgctt tccgaagcgc agccagagct ggagcggacg
     9181 ttactgacga ccgcgttgcg acatacgcag gggcataaac aggaagcggc gcggctactc
     9241 ggctggggcc gcaacactct gacgcgtaag ttaaaagagc tggggatgga gtgattcacg
     9301 gcttgtgtgt aaagattgat tattgagcgc aaattgctgg tattttgcgc tttactgttc
     9361 cgataagttc agtatgatct tgcccggaaa acggggagag tcattatgct ggaatcaata
     9421 attaatctgg tatcgagtgg cgcagttgac agccacacac cgcaaactgc cgttgctgcc
     9481 gtgctgtgtg ccgcgatgat tgggctgttt agctgaagct cgttgtttat gccggatgcg
     9541 gagaaagtcg taggcctgat aagacgcgac cagcgtcgca tcaggcatca tgctcagatg
     9601 cctgatgcgg cgtaaacgcc ttatccggcc tacaaaagca ataatttcaa caggttttgt
     9661 tgcccagcgc atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt
     9721 tgtaaacgaa gcgccattca ctctttaaat cacccgtgag aactgctgca tccgcgcttt
     9781 ctggcgcaga taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc
     9841 cgtcacctgt attcccttct catccacatc caccagccca tcttttgcta acggggcgag
     9901 cagcttgaga tcttccgcaa agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg
     9961 ggcgtaatcc agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc
    10021 atcacgcgtt agcgcaatac cacgccacag cgcattgcct tgttcataca cttgctgata
    10081 gtactgcttc aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga
    10141 aacgcccatc cccagcagat cggtatcgcc ctgagtggtg tagccctgga agttacgatg
    10201 cagcacgcct tcacgctggg ccaccgccag ctcgtcatca ggacgggcaa agtgatccat
    10261 accgataaac tgatagcccg attgcgtcag gaaggcgatg gtttcctgca ggatatcgag
    10321 tttttgctgc ggactcggca ggtcagcatc tttgattttg cgctgagcag caaaaatggt
    10381 cggcagatgc gcgtagttaa aaacgctcag acgatcgggg ttcagctccg ccacacgttt
    10441 cagggtaaag gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt
    10501 ggtggaggta aaaccaatct cacgcgcatg gttaagcagt gcaaagatga actcttcatc
    10561 ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatgctcag
    10621 gcgattaaag ccttcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc
    10681 gacttcgatc gaaatctccg catcggtatt gaaatggaag ttttcgcgca gcagcttcat
    10741 cagacggctg atttgcgctt tattcagata cgtcggcgtt ccgccgcccc agtgcaattg
    10801 gctgacgtga cgcccggcga acagcggtgc acgatggacg atttcttgct ccagcgcgtc
    10861 cagatactga tcggccttgt gctgctggcg agtaacaatc ttattgcaac cgcagaagta
    10921 acaaagctta tggcagaacg ggatatgtac gtagagagat aatggacgct caggatagcg
    10981 cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg
    11041 gtacgaggtg tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc
    11101 gatttgctgt acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc
    11161 ggcggatgcc cgtttcgggt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac
    11221 cgctgtagtt taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga
    11281 acgggaaggc ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct
    11341 tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc
    11401 aactcatcaa tacgatccag tttagcatcc acccaggatt gctcttcggc gctcagggtt
    11461 tcgcctgctt ccagacgttc cagcagcgca tccagacact catccgtttc cagtaattcc
    11521 aactccgcct gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact
    11581 ttttcagtca ctcccaatgg aataggggtt ttactgccaa tacgtggatc ttttggtgcg
    11641 ttctggcctt tgctgcctga cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga
    11701 ccacgacgtt ttttctgacg cttacggtca cgagcttcct gatccagctc ctcgcgtgtt
    11761 tttcgacgcg cttttgcatg acctttgctg cgtgagtttg aagatgatgg tttcataatg
    11821 gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag
    11881 caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg
    11941 ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc ggaacaatcc
    12001 gtgttgattc catttccgtt taattacgtc tccggacgtt tgtcttcctg acaatcctct
    12061 gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc
    12121 gctctatggg tgctacttta cgcctttgct ttaaacaaac aatcaaccgc catcagtatt
    12181 tgcacatttt cagagtttta tataaaagca attcactgat ttatatagct tatttgacat
    12241 gcatcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagcg
    12301 tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat
    12361 aatcgccaca aagctttgac tgacggagac gaccgctttg actaatttga attatcaaca
    12421 gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga
    12481 agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa
    12541 ccagaaaagc ctggctcgta cctcaaaaac cccagggcgc acccagctta tcaacctgtt
    12601 tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc
    12661 ggaagagatg aagcgcaaat ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag
    12721 cctgcaaggt ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca
    12781 gatgattgag tgggcggtag acagcaatat cgccgttctg gtgctgctga ccaaagcgga
    12841 caaactggca agcggcgcac gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc
    12901 gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa
    12961 gctgaggcag aaactggata cctggtttag cgagatgcag cctgtagaag aaacgcagga
    13021 cggcgaataa ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat
    13081 tcaatagatt acaattctac cgttggcctg ataaacatag cgcatcaggc attacggatc
    13141 ttttctttcg cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc
    13201 agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc
    13261 ctacgcgact aactttactc ttttttgttc agttcagaaa gcactttttg taattttttt
    13321 tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta
    13381 cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac
    13441 tccccacttc caccagcaac ggcacatcca gacgggtaca gttttccatc agttgatgaa
    13501 tctgcttcgc gacggcatca acatcatctt tatgaacttc aaataccagt tcatcgtgta
    13561 cctgcatgat catacgtaca cgcggttgct cagcctgtaa ccacgcatca acggcaatca
    13621 tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt
    13681 cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc
    13741 cgtccagcgt ttcaacgtag cccagctctt tcgcctgagc acgggtgcgt tccatatact
    13801 ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg
    13861 gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga
    13921 tcgctttcgc gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt
    13981 ctgccgccgt tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc aagcctttat
    14041 cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca
    14101 cataatcctc tggcgcaata aacgcctggc ggatacgacg accttcttcg ttacgcaccg
    14161 gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcagtt actgcctggt
    14221 gataagaggt atgcacacgc ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt
    14281 aagtcgactt cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt
    14341 ccagcgccag ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct
    14401 tcagcggttt aataccctgt ttttcaaaca gaatggtttg caactgcttg gtggaagaaa
    14461 ggttaaattc ttcacctgca atttcatgcg ctttcttttc cagctcagcc agacgcaagg
    14521 tgagctcttc agaatgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa
    14581 tgcgtgaaag caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt
    14641 tgtgtttctg cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt
    14701 cggcggcgta acgtccggcc tcttcgaggg caatctggtt aaaggtcagt tgatttttgc
    14761 ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc
    14821 tgtccatatc gtgacgcccg gcaacgctat tgagaatgta ggactccagc atggtatcaa
    14881 acgcaatccc acgcagttca atgccgtaat tcgccagaat accgcgatcg tatttcaggt
    14941 tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac
    15001 gctcgtgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg
    15061 ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc
    15121 tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt
    15181 tcagtgtttc ttcatcaagg atagtgacgt agttgtcata agaaatcacc gttgccgtca
    15241 cttctggcgc ttcgtctgca acactggttt cctgaggctt cgcggctgtt tttgcccctt
    15301 tggcctgtaa ccatttgccc acttcgacat cagcagtcca gcgtttgaac tcatactttt
    15361 tgaacagccc caacaactct tccgctgccg gttgctgcac ttccagttgt tcacaggtca
    15421 gctccagttc aacatcggtt ttaatcgtcg ccaactggta tgagagataa gcaacttctt
    15481 tgttttgctc gagcttcgct gccattgttt tcgcgccacg gaagctcaac ccagcaattt
    15541 tttctggctc ggcatacagc gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg
    15601 ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc atcagcgcca
    15661 ggaagtcgat gatcaattct ggcggcacgc cgtacttatt caccacctct tccggtccga
    15721 ggatggtatt cgtcatggta ttgataagcg taatatttgg cgtcaccagc tgcgccatat
    15781 ctttatcgcc agtgctaatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag
    15841 taccgataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt
    15901 taaccatcgc gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat
    15961 gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca
    16021 ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat
    16081 acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc
    16141 gataaagata agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa
    16201 tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat gaaaacatga
    16261 gtttttcacc gcttttcgtg aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc
    16321 atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat catgcttttt
    16381 acagcatgat gtttgtcttt gtttttcatg cagttaaaaa taaatccatc cctaataatt
    16441 gagataatgg gattttttct aatgtggata gatatgaatt atttttctcc ttaaggatca
    16501 tccgttattt gggtcatttt ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc
    16561 tatggctaac cttttgaata aattcattat gacgagaata ctcgctgcga taaccctttt
    16621 gctgagtatc gtattgacca ttttggtcac tattttttgt tccgtcccga tcatcattgc
    16681 cgggattgta aaactattgc tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg
    16741 tgattttatg atgtattgct ggtgtgaagg tctggcggta ttactgcatc tgaacccaca
    16801 cctgcaatgg gaagttcacg ggctggaagg gttaagtaag aagaactggt atctgcttat
    16861 ttgtaaccac cgtagttggg cagatattgt cgtactgtgc gtgctgtttc gtaagcacat
    16921 tccgatgaat aaatatttcc tcaagcaaca gctggcctgg gtgccatttc ttggcctggc
    16981 gtgctgggcg ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt tattacgtca
    17041 tccggagcga cgtggtcaag atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct
    17101 gcatcccacc accattgtca atttcgttga aggctcccgt ttcacgcaag aaaaacatca
    17161 gcaaacccac tccacttttc aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc
    17221 gctaaatgta ctgggtaaac aattcgataa actgcttaat gttacgctgt gttatccgga
    17281 caataaccgt cagccgttct tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca
    17341 tgtggattta cagcctattg ccgatgagtt acatggcgac tacatcaatg acaaaagctt
    17401 taagcgccat tttcagcaat ggctgaactc attgtggcag gaaaaagacc ggcttctgac
    17461 gtctctcatg tcatcacagc gtcagaataa gtaagtttta ctcgcctctg aatgagcaga
    17521 ggcgagtgag tattttaatg aacaaaacgt ccggcacgag acataaattc ttcttcgctc
    17581 atctcctgtc cgttaaaaac aactttaccc ggtgtataac gcagctgcag cgaggcggta
    17641 ttgttgtcaa tcgtgattaa ctgaaacatc tgacctaatg tcatcatccc gctgatttgt
    17701 ttatcagcct gcttttgcgc tttttccgca tccattcctt cagataaatt aagctgtttt
    17761 gcggtttctg tgacgacatt aagcggtaac ttcacatcaa aattgagcga tttgatatct
    17821 ttgtttgtgg atgatgaaga tttggctggg tcggcaatac tgatatccag attggcattt
    17881 agttcgccga gtgcgttctt ccatcttacc ggttgtttaa tggtcggctc acttttttgt
    17941 aataacggtg aatactcttt gaacaatgca gcattcactt cttgcagagc aacttcatcg
    18001 tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata ctggatgata
    18061 aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc accactaccg
    18121 agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac attgatataa
    18181 tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat ttgggcaacg
    18241 tcttccccgt gattgataat gttaatctga ttaaatttcg actcgctttc accaaccgga
    18301 aatttctttt cttctaatcg cgccagagaa tcaatggtaa aactttttgc cgttactttg
    18361 ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc caggttagac
    18421 aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa ttttccatac
    18481 tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc
    18541 gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ttcattattc
    18601 actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg
    18661 atgggcaacg gaccgtgctc aacatccaca tcaaatacaa ctttctggcc tggcttgata
    18721 ttgagatcgg gtgcaccgtt atcgaatgtc atttgcattt gaaaacggga attaaaaaag
    18781 cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc tttctttttc
    18841 gcatcgttaa aatctttaat aaacttttcg ataccaggct gaatttgcgt acctgtgtac
    18901 catgtgccac caccccagat tacggctaac gcaacaataa cacctgtagc tgacttacgt
    18961 atcatataaa tattccctga taaaacagca aaactttgaa tatccactta tgctgaaata
    19021 atgtccagcg gcaggatgca ttatcagaat gtatttatat ttattaaata ataaaaaaag
    19081 cccgtgaata ttcacgggct ttatgtaatt tacattgaat tatttttcct cggacagata
    19141 tttcaccgta tcagcatact gctgaacaaa aacatccata ttgctggtat ccataccctg
    19201 cggattcagc tgatatttac cgttaacaaa catcgccgga acaccacgca attgcacatc
    19261 agctgcggct ttttcctgct gagcgaccag agatttcacc acgaagctgt tccacgccgc
    19321 gtcgtactct tcacctttaa tacctgcatt gataaataca tcgcggatat cagatgctga
    19381 acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt tgtcttccac
    19441 gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt caccacccat
    19501 gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct tcacattatc
    19561 agaaatatgc agaacttctt caaactgata gcagtgcggg cagaagaaag agaaaaactc
    19621 cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt taccatcttc
    19681 atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca gccaaatctt
    19741 tttcatgatc tactctctcc gattaataca taggtgttaa ttgcaaaggg ggttcttgta
    19801 gaacttttgc ctgttctata aaagtcgccg tctgtcgtag ccagtaatct tccccggtta
    19861 accacgggaa atttttcggg aacgcgggat cagcccaatg ccgcattagc caggcaagat
    19921 aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact
    19981 cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc tgggctttat
    20041 cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca tcatccagat
    20101 caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct ccatgtagcc
    20161 gcagaacggt gaaatcttcc cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg
    20221 ctttcagaaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca aacagcttgc
    20281 gaggctcaat gagatattca ttcagaccga tggtcgggcg atgaataaaa agctgtttgc
    20341 gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga tcgatattat
    20401 cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat
    20461 tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca tcatttacca
    20521 gctgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt tcagggcgat
    20581 aaaatttgac gacaaaacgt cgacgctctt cgtcctgaaa ttgatagaca cggttttcat
    20641 agctgttaag cggggtaaga ccggaatcca cccggatccc ttgcttaaac agagcgtcca
    20701 tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc ccatcatccg
    20761 gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc gccgcttaca
    20821 agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca
    20881 taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat tttcaggtga
    20941 tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga ctctttcgcc
    21001 agtttctgca aaaattgcaa caagttaaag tctggctctt tttgccaggc tggctggagg
    21061 agttcaataa cttcattcag acgtttacat ttcatagtaa tgctccttac tcttgagaca
    21121 gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat ctaatgacga
    21181 cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggcggc gtagataaag
    21241 gattgctgga cttaaatggc aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc
    21301 agctctctca cgtcgtggtt aatgctaatc gtcatcagga aatctatcag gcaggcggtc
    21361 tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga atgctttcag
    21421 taatgcagca ggaagctggt gagtggtttt tgttttgtcc gtgcgatacg ccttacattc
    21481 cccatgattt agccgcccgg cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc
    21541 atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt gagcctttat
    21601 tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt ctggctggcg
    21661 gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat acgccagagg
    21721 agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg cgtggagtgg
    21781 taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca gagggatccg
    21841 tccggggctg attaagcata cgcaccatga tatggatgtt gataagccag gcaaagatag
    21901 ctatgagctg cgcaaggctg gcgcggtaca aaccatcgtt gccagccagc aacgatgggc
    21961 cttgatgacg gaaacaccag acgaagaaga gctggatcta cattttctcg caagtcgaat
    22021 ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag agatcgcaaa
    22081 gattgtgctg tttcgcgatg gagccggaca tcgacctgaa gaattagtga tagacaggca
    22141 tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac tggatattaa
    22201 tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga acggataagc
    22261 aagagtaggc tggatcagac gc
//
LOCUS       DAJTRM010000066        21494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRM010000066 DAJTRM010000000
VERSION     DAJTRM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.066"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO322_002664"
     CDS             complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO322_002664"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5907487.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1183..2556
                     /gene="mpl"
                     /locus_tag="OO322_002665"
     CDS             1183..2556
                     /gene="mpl"
                     /locus_tag="OO322_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5907488.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2639..3190)
                     /locus_tag="OO322_002666"
     CDS             complement(2639..3190)
                     /locus_tag="OO322_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5907489.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3284..4636
                     /gene="pmbA"
                     /locus_tag="OO322_002667"
     CDS             3284..4636
                     /gene="pmbA"
                     /locus_tag="OO322_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5907490.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4819..5201
                     /gene="cybC"
                     /locus_tag="OO322_002668"
                     /pseudo
     CDS             4819..5201
                     /gene="cybC"
                     /locus_tag="OO322_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5246..5710)
                     /gene="nrdG"
                     /locus_tag="OO322_002669"
     CDS             complement(5246..5710)
                     /gene="nrdG"
                     /locus_tag="OO322_002669"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5907491.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5868..8006)
                     /gene="nrdD"
                     /locus_tag="OO322_002670"
     CDS             complement(5868..8006)
                     /gene="nrdD"
                     /locus_tag="OO322_002670"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5907492.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8400..10055)
                     /gene="treC"
                     /locus_tag="OO322_002671"
     CDS             complement(8400..10055)
                     /gene="treC"
                     /locus_tag="OO322_002671"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5907493.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10105..11526)
                     /gene="treB"
                     /locus_tag="OO322_002672"
     CDS             complement(10105..11526)
                     /gene="treB"
                     /locus_tag="OO322_002672"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5907494.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11645..12592)
                     /gene="treR"
                     /locus_tag="OO322_002673"
     CDS             complement(11645..12592)
                     /gene="treR"
                     /locus_tag="OO322_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5907495.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12777..12830
                     /gene="mgtL"
                     /locus_tag="OO322_002674"
     CDS             12777..12830
                     /gene="mgtL"
                     /locus_tag="OO322_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5907496.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12971..15667
                     /gene="mgtA"
                     /locus_tag="OO322_002675"
     CDS             12971..15667
                     /gene="mgtA"
                     /locus_tag="OO322_002675"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5907497.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15873..16259)
                     /gene="ridA"
                     /locus_tag="OO322_002676"
     CDS             complement(15873..16259)
                     /gene="ridA"
                     /locus_tag="OO322_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5907498.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16332..16793)
                     /gene="pyrI"
                     /locus_tag="OO322_002677"
     CDS             complement(16332..16793)
                     /gene="pyrI"
                     /locus_tag="OO322_002677"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5907499.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16806..17741)
                     /gene="pyrB"
                     /locus_tag="OO322_002678"
     CDS             complement(16806..17741)
                     /gene="pyrB"
                     /locus_tag="OO322_002678"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5907500.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17745..17879)
                     /gene="pyrL"
                     /locus_tag="OO322_002679"
     CDS             complement(17745..17879)
                     /gene="pyrL"
                     /locus_tag="OO322_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5907501.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18160..18555)
                     /locus_tag="OO322_002680"
     CDS             complement(18160..18555)
                     /locus_tag="OO322_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5907502.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18687..19400)
                     /gene="bdcA"
                     /locus_tag="OO322_002681"
     CDS             complement(18687..19400)
                     /gene="bdcA"
                     /locus_tag="OO322_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5907503.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19471..20064
                     /locus_tag="OO322_002682"
     CDS             19471..20064
                     /locus_tag="OO322_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5907504.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20209..20661
                     /gene="tabA"
                     /locus_tag="OO322_002683"
     CDS             20209..20661
                     /gene="tabA"
                     /locus_tag="OO322_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5907505.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20784..>21494
                     /locus_tag="OO322_002684"
     CDS             20784..>21494
                     /locus_tag="OO322_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5907506.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEK"
BASE COUNT         5191 a         5322 c         5660 g         5321 t
ORIGIN      
        1 gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc aaccatatgg
       61 tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat atccagaata
      121 cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc catcgggttg
      181 cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg gtagagataa
      241 ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat ataacgcgag
      301 gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta cttcttcacc
      361 ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg
      421 aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta agtaaaggcg
      481 tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac cacgtaacct
      541 gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca
      601 ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac atcgatgttg
      661 gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag
      721 acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg tgctttcagt
      781 gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc gccctgcacg
      841 ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg
      901 ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga
      961 gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg
     1021 caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc
     1081 aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca
     1141 tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta
     1201 ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa
     1261 gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc
     1321 attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga tctggtgatt
     1381 attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc
     1441 ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg
     1501 gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg gattctggaa
     1561 cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg
     1621 gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga ctgcgccttc
     1681 ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct caacaacctt
     1741 gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat
     1801 ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga catcaacctg
     1861 aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga gcagggtcac
     1921 tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct ggatggcgaa
     1981 aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa tggcctgatg
     2041 gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa cgcgctgggt
     2101 tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg cgtcacggta
     2161 tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc gctgcgtggc
     2221 aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa taccatgaaa
     2281 atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga agtcttcctg
     2341 ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct
     2401 gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagcct
     2461 ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca gaaactgctg
     2521 gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct gatatagcat
     2581 tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac aacgaaaatt
     2641 acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg
     2701 gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt tgctgacgat
     2761 cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga tcgatcagac
     2821 gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga cggtttttca
     2881 gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc ttaccgatga
     2941 gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca atagccgggc
     3001 gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca acaatttccg
     3061 cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg acccagataa
     3121 tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag tcttcgggct
     3181 gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa gatgcgcact
     3241 gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac ttgcaatgaa
     3301 agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt ccactgcgct
     3361 ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat
     3421 tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg atggcgcgct
     3481 ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg atttaagccc
     3541 gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata cctcgccaga
     3601 tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga atctcgactt
     3661 gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc gcgcagaaca
     3721 ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct ttaacagcca
     3781 ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact gctcaacgcg
     3841 tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc gtgattacgc
     3901 ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg gggccgactg
     3961 tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga aagcgccggt
     4021 catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg cgatagcggg
     4081 tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac aaattctgcc
     4141 ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt cgacgccatt
     4201 cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca tcctgactca
     4261 gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg gacatgcggg
     4321 cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga
     4381 gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg ccattaccgg
     4441 tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc agtatcccgt
     4501 tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg
     4561 taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc cggagatgaa
     4621 aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa aaattacaca
     4681 aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg tactcgcagt
     4741 acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa
     4801 taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg
     4861 gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac
     4921 aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc gttaacgaag
     4981 atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga agataaatca
     5041 ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt cggtcagatt
     5101 gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct gcagagcaac
     5161 tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt tccctgttgc
     5221 ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga ttgctgctgc
     5281 cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg accagcacgt
     5341 taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta tagcctgtcc
     5401 acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt ttcagaatat
     5461 ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc tggcgtttga
     5521 tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta aatggctgac
     5581 cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga acacacccgg
     5641 agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga tagtactgat
     5701 gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc ggtaatcaat
     5761 gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat gtaggccgga
     5821 taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta acctatctgc
     5881 ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc gttaaacgga
     5941 cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac acgggaggcg
     6001 tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa ctcaccggta
     6061 aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg tacatgctga
     6121 tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt tggatactcg
     6181 ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa gtcgatcttg
     6241 tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta gtaaccttta
     6301 tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa gcggtcgcac
     6361 aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc tttccactga
     6421 tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag cttctcgttg
     6481 tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc gatgtaaccc
     6541 agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc agcattcaga
     6601 cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt cacgccttcg
     6661 agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc atccagcagc
     6721 ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag gttcaggctg
     6781 atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt ttcccacacg
     6841 ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt cactacctga
     6901 tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc cagctgtttg
     6961 atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc aaacaccagt
     7021 ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt acgcaggatc
     7081 gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa ggttacaaac
     7141 ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga ctggaaggca
     7201 tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg gatgttccac
     7261 tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc cagcacttca
     7321 tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc aataatctgc
     7381 gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc gttccccatt
     7441 ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa catcgggaag
     7501 aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc ctgcaccacg
     7561 tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc cagcaggtcg
     7621 cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa cgaggcgttg
     7681 gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc acgttcaatg
     7741 tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg acccgacatc
     7801 agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg cccctgcatc
     7861 tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac ttccgctgct
     7921 ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac tttgcagccg
     7981 tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca ggttatccac
     8041 agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct ttccgcaatc
     8101 gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta gcaattatag
     8161 tcgattaata caacatattg ggttgggacg cattttaaag tctatatata gtgctttgca
     8221 tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc gtacggatga
     8281 cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa atcaacaaaa
     8341 acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt agggaagatt
     8401 tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca gggttgtggt
     8461 gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg ccctggctgc
     8521 cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc cttccattca
     8581 cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt gccccatgtc
     8641 aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa cacagaggag
     8701 tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag gccaatccac
     8761 ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg cgtgcggctg
     8821 ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg accatcgttg
     8881 cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat gcgcgtgaaa
     8941 tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc ctgcatgcca
     9001 tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc atcaccaaag
     9061 cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca tgccacgttg
     9121 tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc aggtttagcc
     9181 agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt aaaggtcatc
     9241 gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct ggtggaggac
     9301 atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat ctcgtgcaaa
     9361 aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc caggtcttca
     9421 gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc gtcgacccca
     9481 cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg attctcccag
     9541 ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct ttccgcatgc
     9601 cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt ttctggttcg
     9661 ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc ctcgcgaaac
     9721 caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg aatcccgcgc
     9781 gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt gggatcaatc
     9841 gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata aaagggggtt
     9901 agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat aacgccacgt
     9961 aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat ctggtagata
    10021 acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca aatgcgaggg
    10081 ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc ggtatttccg
    10141 ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca ttgccagcgc
    10201 aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc ctacgccgat
    10261 accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag aaccaatcat
    10321 cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag gctcagtgac
    10381 cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt gcttgcggct
    10441 ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg caatcagcgg
    10501 ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa gggtggtctg
    10561 gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg caccaatcgg
    10621 agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc caatcatgcg
    10681 accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg aacagacggg
    10741 caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt caataacgcc
    10801 cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca ctttggcgat
    10861 gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat aagcgttcat
    10921 cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg tgccgcccat
    10981 ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg cttcaccgat
    11041 caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca gcgtctgacc
    11101 gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac cgccgctaat
    11161 caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact gctcatgcca
    11221 tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg cctgtccggt
    11281 tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca cctgaaattg
    11341 cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt tcggtctggc
    11401 attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca ccgtcgcaat
    11461 attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt ttattttgct
    11521 catcataaag ccccatggca gatgacattt ttggttggct gcagaatata ttgcgcggaa
    11581 aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat cgttcaggaa
    11641 acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc ccggttacct
    11701 gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga tctacggtga
    11761 cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc agttgcaagg
    11821 tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg tcggttgcgc
    11881 acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag ccttgcttca
    11941 tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag aacgccaggt
    12001 aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg agataactga
    12061 tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc ccttcgtcgt
    12121 cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc gatgactgcc
    12181 agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg ccgtcgatat
    12241 tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac tgattttcca
    12301 tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga acggcgagat
    12361 tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg ctttgcccac
    12421 gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact gcttcaacac
    12481 gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa accgtagatt
    12541 tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc atcctgttgt
    12601 cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca gcaatgggta
    12661 aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata aatgctacga
    12721 atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga ggttatatgg
    12781 aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa gcccgtcttt
    12841 tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc agctttatta
    12901 ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag acagtgcgga
    12961 gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac cttcccgtct
    13021 ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg tcccgccgtc
    13081 cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt ggaaaacgtt
    13141 cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg aacaacatgg
    13201 tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat gggtctgcta
    13261 tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg ccacggaaga
    13321 tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc tgaactttat
    13381 tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca gtaataccgc
    13441 gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga tcccgataga
    13501 ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc cggcagattt
    13561 acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg gtgagtctct
    13621 gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc tggagtgcga
    13681 caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa tggtgattgc
    13741 tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc aggaaagcga
    13801 gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc gctttatgct
    13861 ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact ggtgggaagc
    13921 ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc cgatgattgt
    13981 tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga tcgtcaaaca
    14041 tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata aaaccggcac
    14101 cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta aaaccagcga
    14161 acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta aaaatctgct
    14221 tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg ccagccgttg
    14281 gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg tggtagtggc
    14341 agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa tcctcaatgt
    14401 gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca tgctgcgtaa
    14461 gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg cggtggcgac
    14521 gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg acctgatcct
    14581 cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg cactgaaggc
    14641 attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt tagttgcagc
    14701 gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta gtgatattga
    14761 aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt ttgcccgcct
    14821 gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc atgtggttgg
    14881 ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata tcggcatttc
    14941 tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc tggaaaaaag
    15001 cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca acatgctgaa
    15061 atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc tggtggcgag
    15121 tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga acctgctgta
    15181 cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa ttcaaaagcc
    15241 gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac cgatcagctc
    15301 gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca acacgccgga
    15361 aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc aaacgctgat
    15421 tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat cgtggccgtt
    15481 aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt caccgctggc
    15541 cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg ttgcgattct
    15601 ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc gttacggctg
    15661 gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa agtgagcttt
    15721 gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata aattgcagga
    15781 atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca tcagacttaa
    15841 tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat tgcttcgatc
    15901 tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc cgggaaagtg
    15961 gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa gtcgttcaga
    16021 tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc ggcttcgacg
    16081 attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc cggtacttca
    16141 ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc cagatcaacg
    16201 ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt tttgctcatg
    16261 atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca gacttttatt
    16321 accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc acagtatttg
    16381 catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga aaccggttcg
    16441 gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg ctctggcaga
    16501 cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt ggcttgcggt
    16561 gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc gattttgatc
    16621 agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat gcgctgatct
    16681 gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg gatatggtca
    16741 attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt catctctatt
    16801 tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg gcgagcgaaa
    16861 atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac atccgtcgca
    16921 atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc gttgtggaga
    16981 tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg gtccagacgc
    17041 tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc ttcaatagag
    17101 ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta ttgcggcatt
    17161 gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc tagcgcctgg
    17221 gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac gtggagattg
    17281 tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg cgtcggatgt
    17341 tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc ggtggctagg
    17401 cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt gctgataacc
    17461 gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc gctgtcggag
    17521 aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag gcgggtacgg
    17581 gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc tggttgcggg
    17641 tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg actaaggtcg
    17701 tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct cctgacacct
    17761 aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg gaagaaaaac
    17821 ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca ctgaaccata
    17881 catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc aagcatttga
    17941 tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata tatgcatttt
    18001 tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca gacatttcct
    18061 gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt catccgactt
    18121 atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag ggatacgcgc
    18181 tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg ccgtccagtt
    18241 tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt cagattgttt
    18301 ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc atcccgcagc
    18361 tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa aatcgggttc
    18421 tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat cgccggaacg
    18481 aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag ggaaaacagc
    18541 ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag atttatattt
    18601 atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat catggataat
    18661 ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat ggtatgcatc
    18721 gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc agcgacctct
    18781 tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg cattggcccg
    18841 ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat ggtgatccct
    18901 cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga tttactggcg
    18961 gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc gatgattaag
    19021 atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata aggagcatga
    19081 atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc atcgccaaag
    19141 acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg aacgacatca
    19201 ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt ctcttgtgcc
    19261 agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc cccatcggtg
    19321 acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat gagaactgtc
    19381 ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc attacaaaac
    19441 tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc tcgcgttcca
    19501 ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt gctttttcac
    19561 caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg tattaacccc
    19621 ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt actcaatgaa
    19681 tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg tccggtaggc
    19741 gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa cggcggctgc
    19801 gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg tgatattgcc
    19861 attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag gcggcatcca
    19921 caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct ttctgcgaag
    19981 gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc aggggaagcg
    20041 ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga acgcgcctgc
    20101 cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg atcatctttt
    20161 cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat gatcatcgga
    20221 aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat tgagcatatc
    20281 aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg caatcgtctg
    20341 ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc ggagtaccat
    20401 gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac cttcagcacg
    20461 caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc atttttgccg
    20521 gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt gttttatccg
    20581 ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg caaagcagta
    20641 gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt tacggcgagt
    20701 ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg cttagacgtt
    20761 cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat tctcaacaca
    20821 ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg tatgtgggaa
    20881 tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt ctttctccta
    20941 gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat tttagcaaac
    21001 gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct tattctaaag
    21061 atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg gagcgagcgg
    21121 atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa gggattcgtg
    21181 aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt tatgaaggga
    21241 agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag gttctggcgt
    21301 gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat cagaagttaa
    21361 ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag ttaaataatt
    21421 cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct acgattacgg
    21481 atttattaga aaaa
//
LOCUS       DAJTRM010000067        21256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRM010000067 DAJTRM010000000
VERSION     DAJTRM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.067"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            20..793
                     /locus_tag="OO322_002685"
                     /pseudo
     CDS             20..793
                     /locus_tag="OO322_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="OO322_002686"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="OO322_002686"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5907507.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OO322_002687"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OO322_002687"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5907508.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OO322_002688"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OO322_002688"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5907509.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="OO322_002689"
     CDS             complement(4991..7225)
                     /locus_tag="OO322_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5907510.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OO322_002690"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OO322_002690"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5907511.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="OO322_002691"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="OO322_002691"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5907512.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OO322_002692"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OO322_002692"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5907513.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="OO322_002693"
     CDS             complement(12980..14320)
                     /locus_tag="OO322_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5907514.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OO322_002694"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OO322_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5907515.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="OO322_002695"
     CDS             complement(16108..16557)
                     /locus_tag="OO322_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5907516.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OO322_002696"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OO322_002696"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5907517.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="OO322_002697"
     CDS             complement(17357..17686)
                     /locus_tag="OO322_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5907518.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OO322_002698"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OO322_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5907519.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="OO322_002699"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="OO322_002699"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5907520.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="OO322_002700"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="OO322_002700"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5907521.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5672 c         5068 g         5288 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccatcata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctccc
//
LOCUS       DAJTRM010000068        21052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRM010000068 DAJTRM010000000
VERSION     DAJTRM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.068"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..2009)
                     /gene="glyS"
                     /locus_tag="OO322_002701"
     CDS             complement(<1..2009)
                     /gene="glyS"
                     /locus_tag="OO322_002701"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5907522.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLT"
     gene            complement(2019..2930)
                     /gene="glyQ"
                     /locus_tag="OO322_002702"
     CDS             complement(2019..2930)
                     /gene="glyQ"
                     /locus_tag="OO322_002702"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5907523.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3025..3324)
                     /locus_tag="OO322_002703"
     CDS             complement(3025..3324)
                     /locus_tag="OO322_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5907524.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3499..4494
                     /gene="wecH"
                     /locus_tag="OO322_002704"
     CDS             3499..4494
                     /gene="wecH"
                     /locus_tag="OO322_002704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5907525.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4536..4973)
                     /locus_tag="OO322_002705"
     CDS             complement(4536..4973)
                     /locus_tag="OO322_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5907526.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5019..5360)
                     /locus_tag="OO322_002706"
     CDS             complement(5019..5360)
                     /locus_tag="OO322_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907527.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5529..6983)
                     /gene="xylB"
                     /locus_tag="OO322_002707"
     CDS             complement(5529..6983)
                     /gene="xylB"
                     /locus_tag="OO322_002707"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5907528.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7055..8377)
                     /gene="xylA"
                     /locus_tag="OO322_002708"
                     /pseudo
     CDS             complement(7055..8377)
                     /gene="xylA"
                     /locus_tag="OO322_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8743..9735
                     /gene="xylF"
                     /locus_tag="OO322_002709"
     CDS             8743..9735
                     /gene="xylF"
                     /locus_tag="OO322_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5907529.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9813..11354
                     /gene="xylG"
                     /locus_tag="OO322_002710"
     CDS             9813..11354
                     /gene="xylG"
                     /locus_tag="OO322_002710"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5907530.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11332..12513
                     /gene="xylH"
                     /locus_tag="OO322_002711"
     CDS             11332..12513
                     /gene="xylH"
                     /locus_tag="OO322_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5907531.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12591..13121
                     /locus_tag="OO322_002712"
     CDS             12591..13121
                     /locus_tag="OO322_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5907532.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13233..13769
                     /locus_tag="OO322_002713"
     CDS             13233..13769
                     /locus_tag="OO322_002713"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5907533.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13877..14701)
                     /locus_tag="OO322_002714"
     CDS             complement(13877..14701)
                     /locus_tag="OO322_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5907534.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15021..17051
                     /gene="malS"
                     /locus_tag="OO322_002715"
     CDS             15021..17051
                     /gene="malS"
                     /locus_tag="OO322_002715"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5907535.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17229..18482
                     /gene="avtA"
                     /locus_tag="OO322_002716"
     CDS             17229..18482
                     /gene="avtA"
                     /locus_tag="OO322_002716"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5907536.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18633..19106)
                     /locus_tag="OO322_002717"
     CDS             complement(18633..19106)
                     /locus_tag="OO322_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5907537.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19208..20056)
                     /gene="yiaJ"
                     /locus_tag="OO322_002718"
     CDS             complement(19208..20056)
                     /gene="yiaJ"
                     /locus_tag="OO322_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5907538.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20256..>21052
                     /gene="yiaK"
                     /locus_tag="OO322_002719"
     CDS             20256..>21052
                     /gene="yiaK"
                     /locus_tag="OO322_002719"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5907539.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIA"
BASE COUNT         5291 a         5361 c         5164 g         5236 t
ORIGIN      
        1 gtcagacggt tgagacgcaa ttctttgtca tcaaccatga ccatcacttt atcgaagaaa
       61 gcatcaaccg gctcacgcag ctctgccagt tcgaccagcg catcctggta acgaccttcc
      121 gcaaagtacg gctccagctt gtcacgtagc accacaacct gcatcgccag tttaatttct
      181 tccggctctt tcagggtaga ggcattcacg cgatcgctca gcacttcgtc agatttcgcc
      241 aggatgttag atacacgttt gttcgccgcc gccagtgctg cagctgcttc cagggtacgg
      301 aagtgcgata ccgctttcat gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc
      361 agtaccgcct ggatggtgtc aacggtataa ccttcgtcct gataccaggc gcggaagcga
      421 ccgagcataa agtcgataac atcatcaact acgttggcat tagtcagctt atcgccatac
      481 agacgcaccg cttcttcggt cagcgtttgc agatcaaggt tgaggttctt ctcaacgata
      541 attcgcagca cgccaagtgc ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga
      601 tgctgaccga taccgaagat acccgccaga gtatccatct tatcagcaat cgccagcgca
      661 caagctaccg ggttggacgg caggtcatca ccggcaaagc gcggctgata ctgctcattc
      721 agcgcgacgg ctacgtcttc cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg
      781 ccttgggtgt cggtgaactc gaagaccatg ttggtcatca ggtcacactt agacagcagg
      841 cccgcacggg ttgcgtggtt aacgtcagcg ccaatctgtt cggcgatcca gccagccagc
      901 gcctggatgc ggtcagtctt gtcacgcagc gtacccagct gttgctggaa cagcacggtt
      961 tgcaggcgcg gcaggttatc ttccagacgt tttttgcggt cggtgttgaa gaagaactcg
     1021 gcatccgcca gacgtggacg aacgactttc tcgttaccag agataatttg ctgcggatct
     1081 ttcgattcga tgttggcaac gaagataaag ttcggcagca gtttgccgtc gttcgcatac
     1141 accgggaagt acttctggtc acctttcatg gtgtaaacca gggcttcagc cggcaccgcg
     1201 aggaatttct cttcgaattt tgcggtcaga acgaccggcc actccaccag cgaagccact
     1261 tcttccagca ggctttcgct taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg
     1321 gcatcggctt taatcttcgc tttacgttct tcgtaatcgg cgatgacttt cccgcgctca
     1381 cgcagaattt ccggatactg atcggcgttg tcgatggtga attccggttc gcccataaag
     1441 cggtggccgc gaatcacgcg atcggactga atgcccagaa tggttgccgg aatgactttg
     1501 tcgcccagca gcagggtcac ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc
     1561 cagcgcatca gtttcgggat cggcagtttc gccagcgaag tcgcaaccat attcggcagc
     1621 agtgcttcgg tgctttcgcc cttcacatgg gcgcgataca gcagccattc gcctttatcg
     1681 gtagtcagac gctcagcctg gtcaacggta ataccacaac cacgcgccca accttctgcc
     1741 gctttgctcg gttttccttc agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg
     1801 atttcacgat ctggttgtgc ttccgccagg ttagctactt tcagcgccag acgacgcgga
     1861 gcagcaaacc attgaacggt gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag
     1921 ttcgcagcaa aggactctgc caggctgcgc agtgcttttg gtggcagctc ttcagtgccg
     1981 atttccacca gaaaagtttt ctcagacata gccgcctctt acttatcttt gttgcacatc
     2041 gggaagccga gggcttcacg ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg
     2101 cgaatgcgca gaatgtagcg ctgacgctcg gtgacggaga tggctttacg cgcatccagc
     2161 aggttgaagc tgtgggcggc tttcagaata cgctcgtagg ctggcagcgg cagcggattt
     2221 tccagcgcca gcagctgctg cgcttctttc tcgtactgct caaagcaggt gaacaggaag
     2281 tccacatccg cgtattcgaa gttgtaagtg gactgctcca cttcgttctg atggaacacg
     2341 tcgccgtagg tggttttacc cagcgggccg tcgctccaga ctaggtcgta aacgctgtct
     2401 acgccctgaa tgtacatggc cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt
     2461 ttacactcca gaccaccaac ctgctggaag taagtgaact gcgtcacttc catgccgttt
     2521 agccacactt cccagcccag tccccaggcc cccagagtcg ggttttccca gttatcttcc
     2581 acgaaacgga tgtcgtgaat agtcgggtcc atgcccagct ctttcagaga accgaggtac
     2641 agctcctgaa tattgtccgg cgatggctta atgaccacct ggaactgata gtagtgctgt
     2701 aaacggttgg ggttttcgcc gtagcgacca tcggtcgggc gacgagaagg ctgaacataa
     2761 gcagccgcca tcggttctgg ccccagcgcg cgcagacagg tcattgggtg agaggttccc
     2821 gcgccgactt ccatgtccaa tggttgaaca atggtgcagc cctggcgagc ccagtaatcc
     2881 tgtaaggtca ggatcaagcc ctggaaggtc ctggtatcaa acttttgcat attatttcgt
     2941 gctggatacg tgtggattta aaggaaggga tcagtatacc cgctggatgg aagatataca
     3001 gtacgaaacg ggaaaaagca gggcttaacg catggaaaga tgcaaaaaat ggccatctgc
     3061 gtcaaaagaa cagacaaact gctctttacg gaaggtataa ccgcgcattt catagcttcc
     3121 ctggaactgt tcgaaggcgg tgacgtcgat tttttgctcg ccggtgttat aacgttttgc
     3181 cgcctgatcc ttgcataacg cttccatatt caacgaacgc agaggatcaa cccttacccg
     3241 ttgtgctttc tgcacgggtg acggtgtaga acaacccact agcaccataa gcgccattgc
     3301 cggaaagaaa gcgttcatca tcattaaatt accgtctggt ttgcaggtga gtcttattat
     3361 ttatatcggt atagtggctg atatcttacg aatttcctta agcctctata ggctgtagtg
     3421 ataaaactca gattattcca gtgcgccaac caaatgattt atcggtcaga ctttttttaa
     3481 ccagatacag agacacagat gcagcccaaa atttactgga ttgataacct gcgagggata
     3541 gcgtgtttaa tggtggtgat gattcacacc actacctggt atgtgaccaa tgctcatagt
     3601 gttagccccg tcacatggga tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg
     3661 ccgctatttt tcatgatttc cggctatctc ttttttggcg aacgcagcgc ccagccgcgc
     3721 catttcttgc gtatcggctt atgtctgatt ttttatagcg caatcgcact gctctacatt
     3781 gcgctgttta cctccatcaa tatggagtta gcgctgaaaa acctgctgca aaagccagtg
     3841 ttttaccact tgtggttttt cttcgcgatt gcggtgattt atctggtttc accgctgatt
     3901 caggtgaaga acgtcggcgg aaaaatgttg ctggtgctaa tggtggtgat tggcattatc
     3961 gctaacccaa acacagtgcc gcagaaaatt gacggttttg aatggctgcc aattaactta
     4021 tatatcaatg gcgatacttt ttactacatt ctgtatggca tgttgggccg cgctataggg
     4081 atgatggaca cacagcataa agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg
     4141 gtttttatta tctctcgcgg gacattatat gaattgcagt ggcgcggaaa ttttgccgat
     4201 acctggtatc tttactgtgg gccgatggtt tttatctgcg caatcgcgct attgactctg
     4261 gttaaaaaca cgctggatac gcgtaccatt cgcggacttg gcttaatctc ccgccattca
     4321 ttgggtatat acggattcca cgccttgatt atccatgcgc tgcgcacccg gggaattgag
     4381 cttaaaaatt ggccaatact ggatattatt tggatctttt gcgcgacgtt ggcagcgagt
     4441 ttgttacttt ctatgctggt acaacgaatc gacagaaaca gattagtgag ttaagtaaaa
     4501 gcccgttcac attggactga ccgggcttac gtgaattatt cgctgtattc ttcctgggta
     4561 acctgtgttt cttttggcgg gtttattccg gcatcacgaa tattcttctg caccgctact
     4621 gcaccaaaca agcttaagaa gaaagccagt ccataaaaac ctttttcact gagtaataat
     4681 gtcgcattcc acagacctac catcagtaga gcaacagaga taataaagac agtcaggcag
     4741 gtcatataat aaatggaagt ggtcggtatg ccttcatact tgtcccgaac ggtcttttga
     4801 taagacgcag cagaaaacag tcctaacacc agtacggcaa aataataccc tttttcattt
     4861 aactgcatct ctgcattcca tagccctaac aggtaggtaa cgataccacc aacgagagct
     4921 atccatgaca caatactaaa ggccggtgaa taggttgata ttttgttgtc catcacagta
     4981 ttcctttcat tcctgaatgt gtaagagctt tatgttgctc ataccgagcg aaacaaacgc
     5041 atttgaccaa acaagcagac aaaaaaggcg atggggtaga tcaccatttc ataagcattg
     5101 ataggtgcat ttaaaacacc gataaacaag agaccgatag tgattaacgc aactaactgg
     5161 cagatatggg taaaaaaatt atccagttgc ccgagttttt ctgcgcgaag atatgcatag
     5221 ttaccaaaag ttgctgtcat taacacgcca agaaaatagc cttttccact tagcaatggg
     5281 catgagatcc atagcccaac cagataaacc agcgccccga caacaaataa tagttttgcc
     5341 acagtttttg aggttttcat aacgatctcc atatctacca gcgatacatt acgagtaacc
     5401 aacgaaagac aaaactgaaa aatgccatta acaaatgatt ttcagaataa attcatacta
     5461 aatattaatt aattagtgag atatatagat gtgaattatc ccccacccgg tcaggcaggg
     5521 gataacgttt acgccattaa tggcagaagt tgctgataga ggcgacggaa cgtttcacgt
     5581 cgtggctgat aagcggcata acgctgcgca tctggtagat gcgactgttc taacggtagc
     5641 tgcggcaaca actcaatgag cgatttctct ggattcgccg caatctgcgc cagccttgct
     5701 gcgcccagtg ctggccccac atcccccccc gtacggtaat cgagctgctg accgctgata
     5761 tccgccagca tctgacgcca gtactcacta cgcgccccgc caccaatcaa cgtaacactt
     5821 tgcggtttaa tgccgcaggc atgcacgaca tccatgccat ctgccagcgc ataacccacg
     5881 ccttccagca ctgctcgcgc cagttcattg gggccatgtt gatgagtcaa accaaagaaa
     5941 acccccttcg cctggggatt attgtgtggt gtacgctcgc cggaaagata aggcagaaac
     6001 caaactggct cggcactttc atcagcctgt tgagctgcag cgattaaagc tgggacattg
     6061 cacaggccgg ttaatttcgc ggcccaatcc agacacgatg ctgcactcag cattacagac
     6121 attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt gtacagcgct ttctggcttg
     6181 cttaagaacc cttcgctgac agcaaaatag acccccgacg tccccagcga taacattgcc
     6241 tgattagcat caaccattcc cacaccaact gcaccagctg cattgtcgcc accgcctgcg
     6301 acaactggca ccgtcgccat accccacgct ttcgcaactt caggtaacaa agcaccagta
     6361 atttcgctgc cttcgtataa tgcgggcatc tggtcacgag ataagtcgca agcctgcagc
     6421 atgacgtcac tccagtcacg ctttgcgaca tccagccaca tggtgccagc tgcgtcagac
     6481 atatcgctgg caaactcccc cgtcatacgc agacgcaagt aatctttcgg taataatact
     6541 ttgtcgattt gacggaatat ctccggctca tgccgctgaa cccatagcaa tttaggcgca
     6601 gtaaatccgg gcatcatcag gttgccggta atcactcgtg attgcggaac tctcgcttcc
     6661 agcaaagtgc actcttgcgc acagcgcccg tcgttccaca aaatggcagg gcgcaatacc
     6721 cgttgttgag catccagtaa ggttgctcca tgcatctggc cggcaatacc caatgcttta
     6781 acgtcctgca gagaatgctg atcgcccaga gctttcattg cgcgatcagt tgcctgccac
     6841 cactgttccg ggtcttgttc cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc
     6901 gtttgcgaag caaccacctc accctgctcg ttgagcaaaa taacttttac gcccgaggtg
     6961 ccaagatcta tcccgatata catatcgatc gttccttaaa aaaatgcccg gtatcgctac
     7021 cgataaccgg gccaacggac tgcacagtta gccgttattt gtcgaacaga taatgattta
     7081 ccagattttc cagttgctcc tggcgaccac tctgatgcac cggagacaaa ttatgttcct
     7141 gagcatattt ggctaaatct gccagtgaca tttggccttt caggatttgc tggcccaatt
     7201 cgctattcca gccggaataa cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca
     7261 tgcgcgctgc aattttcagc gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa
     7321 acagatcata tttatcagta ctttgacgac gtactttggc atcgaagttc agaccaccgg
     7381 tggtgaaacc gcctgctttg agaatttcat acatcaccag cgcattctct tccacactgt
     7441 tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc acggttggcg tcgacagaac
     7501 cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg gaaagagtga cctgccagcg
     7561 tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc cagaccaaac tgtttcagga
     7621 agccatagac cgtcgcggca tcgtaatcat attgatgttt agtcggttct tgcggtttcg
     7681 gttcgataag caacgtgccc tagaagccga ttttatgttt atgctcaacc accatctgca
     7741 taaagcggcc cagttgttca cgctcctgac gcaagtcggt atttaacagc gtttcgtaac
     7801 cttcacgacc accccacagg acatagtttt caccgcccaa tttatgggtt gcttccatcg
     7861 ctgtaacaac ttgcgttgcc gcccagctga agacttcagg atctgggttc gtcgccgcac
     7921 ccgcgccata gcgagggttt gtaaagcagt tagcggttcc ccacagcagc ttcacgccgc
     7981 tctcttcttg cttggctgcc aggacatcaa ccatttgcgc aaaattattg atgtactctt
     8041 ttaacgacgc gccctcaggg gaaacatcca catcgtggaa gcaataaaat ggcacatgta
     8101 acttgtggaa aaactcaaat gcgacatctg ctttacgctt cgccaacgcc agtgcctcac
     8161 caggctgctg ccacggacga ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc
     8221 agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca
     8281 ccagttcgtc gggattgtag tgacggaatg ctaacgggtt tgaggatttt gagccttcat
     8341 aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt gaactccata atcaggtaat
     8401 gccgcgggtg atggatgatg tcgtaatatt gggcactccc tttcaattgc tcaattatgt
     8461 tatttcacac tgctattgag ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg
     8521 atgatgaaaa tgggtaattc ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa
     8581 agtaagatct cggtcataaa tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat
     8641 ctgtaattgt tttcccctgt ttagttgcta aaaattggtt acgtttatcg cggtgattgt
     8701 tacttattaa agctgtcctc taactacaga aggccctaca ccatgaaaat aaagaacatt
     8761 ctactcaccc tttgcacctc acttctgctt accaacgttg cggcacacgc caaagaagtc
     8821 aaaataggta tggcgattga tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt
     8881 tttgtgaaaa aggcagaatc tctcggtgcg aaagtatttg tacagtctgc aaatggcaat
     8941 gaagaaacac aaatgtcgca gattgaaaac atgattaacc ggggtgtcga tgttcttgtc
     9001 attattccgt ataacggtca ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt
     9061 attaaagtat tagcttacga ccgtatgatt aacgatgcgg atatcgattt ttatatttct
     9121 ttcgataacg aaaaagtcgg tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa
     9181 ggtaattact tcctgatggg cggctcgccg gtagataaca acgccaagct gttccgcgcc
     9241 ggacaaatga aagtgttaaa accttacgtt gattctggaa aaattaaagt cgttggtgac
     9301 caatgggttg atggctggtt accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc
     9361 gccaataata acaaaattga tgctgtagtt gcctcaaacg atgccaccgc aggtggggca
     9421 attcaggcat taagcgcgca aggtttatca gggaaagtag caatttccgg ccaggatgcg
     9481 gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa ctatgacggt gtataaacct
     9541 attacattgc tggcaaatac tgccgcagaa attgccgttg aattgggcaa tggtcaggag
     9601 ccaaaagcag atacctcact gaataatggc ctgaaagatg tcccctcccg cctcctgaca
     9661 ccgatcgatg tgaataaaaa caacatcaaa gatacggtaa ttaaagacgg attccacaaa
     9721 gagagcgagc tgtaagcgtt acgccccggc gcggagcggg ggcgtgattt ctctccatgc
     9781 cgcgtgaatg aattggctta ggtggagtcg ttatgcctta tctacttgaa atgaagaaca
     9841 ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg
     9901 gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc
     9961 tgtgtggtat ttatccccat ggctcctacg aaggcgaaat tatttttgcg ggagaagaga
    10021 ttcaggcgag tcacatccgc gataccgaac gcaaaggtat cgccatcatt caccaggaat
    10081 tggccctggt gaaagaattg accgtgctgg aaaatatctt cctgggtaac gaaataaccc
    10141 acaatggcat tatggattat gacctgatga cgctacgcta tcagaagctg ctcgcacagg
    10201 tcagtttatc catttcacct gatacccgcg ttggcgattt agggcttggg caacaacaac
    10261 tggttgaaat tgccaaggca cttaataaac aggtgcgctt gttaattctc gatgaaccga
    10321 cagcctcatt aactgagcag gaaacgtcgg ttttactgga tattattcgc gatctacaac
    10381 agcacggtat cgcctgtatt tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg
    10441 atacgatttg cgttattcgc gacggacagc acattggtac gcgtgatgct gccggaatga
    10501 gtgaagacga tattatcacc atgatggtcg agcgagagtt aaccgcgatt taccctaatg
    10561 aaccacatac caccggagat gaaatattac gtattgaaca tctgacggca tggcatccgg
    10621 tcaatcgtca tattaaacga gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac
    10681 tgggtattgc cggactcgtt ggtgccggac gtaccgagac cattcagtgc ctgtttggcg
    10741 tgtggcccgg acaatgggaa ggaaaaattt atattgatgg caaacaggta gatattcgta
    10801 actgtcagca agccatcacc caggggattg cgatggtacc cgaagacaga aagcgcgacg
    10861 gcatcgttcc ggtaatggcg gttggtaaaa atattaccct cgccgcactc aataaattta
    10921 ccggtggcat tagccagctt gatgacgcgg cagagcaaaa atgtattctg gaatcaattc
    10981 agcaactcaa agttaaaacg tcgtcccccg accttgctat tggacgtttg agcggcggca
    11041 atcagcaaaa agcgatcctc gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg
    11101 atgaacctac caggggtatt gatattggcg cgaaatatga gatctacaaa ttaattaacc
    11161 aactcgtcca gcagggtatt gccgttattg tcatctcttc cgaattacct gaagtgctcg
    11221 gccttagcga tcgtgtactg gtgatgcatg aagggaaact aaaagccaac ctgataaatc
    11281 ataacctgac tcaggagcag gtgatggaag ccgcattgag gagcgaacat catgtcgaaa
    11341 agcaatccgt ctgaagtgaa attggccgta ccgacatccg gtggcttttc tgggctgaaa
    11401 tcactgaatt tgcaggtctt cgtgatgatt gcagctatca tcgcaatcat gctgttcttt
    11461 acctggacca ccgatggtgc ctacttaagc gcccgtaacg tctccaacct gttacgccag
    11521 accgcgatta ccggcatcct cgcggtagga atggtgttcg tcataatttc tgctgaaatc
    11581 gacctttccg tcggctcaat gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc
    11641 tggttaggct ggcctttgcc acttaccatc attgtgacgt tggttctggg actgcttctc
    11701 ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc
    11761 gcgggcatgt tggcatttcg cggcatactc attggcatca ccaacggcac gactgtatcc
    11821 cccaccagcg ccgcgatgtc acaaattggg caaagctatc tccccgccag taccggcttc
    11881 atcattggcg cgcttggctt aatggctttt gttggttggc aatggcgcgg aagaatgcgc
    11941 cgtcaggctt tgggtttaca gtctccggcc tctaccgcag tagtcggtcg ccaagcttta
    12001 accgctatca tcgtattagg cgcaatctgg ctgttgaatg attaccgtgg cgttcccact
    12061 cctgttctgc tgctgacgtt gctgttactc ggcggaatgt ttatggcaac gcggacggca
    12121 tttggacgac gcatttatgc catcggcggc aatctggaag cagcacgtct ctccgggatt
    12181 aacgttgaac gcaccaaact tgccgtgttc gcgattaacg gattaatggt agccatcgcc
    12241 ggattaatcc ttagttctcg acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa
    12301 ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc
    12361 gttgccggag cagtaatggg ggcatttatc atggcttcac tggataacgg catgagtatg
    12421 atggatgtac cgaccttctg gcagtatatc gttaaaggtg cgattctgtt gctggcagta
    12481 tggatggact ccgcaaccaa acgccgttct tgattttgat aaaaattttc tcaaagccgg
    12541 ttacgtatta ccggttttga gtttttgcat gattcagcag gaaaagaacc atgtttacta
    12601 aacgtcaccg catcacatta ctgttcaatg ccaataaagc ctatgaccgg caggtagtag
    12661 aaggcgtagg ggaatattta caggcatcac aatcagaatg ggatattttc attgaagaag
    12721 atttccgcgc ccgcattgat aaaatcaagg actggttagg agatgtcgtc attgccgact
    12781 tcgacgacaa acagatcgag caagcgctgg ctgatgtcga cgtccccatt gttggggttg
    12841 gcggctcgta tcaccttgca gaaagttacc cacccgttca ttacattgcc accgataact
    12901 atgcgctggt tgaaagcgca tttttgcatt taaaagagaa aggcgttaac cgctttgctt
    12961 tttatggtct tccggaatca agcggcaaac gttgggccac tgaacgcgaa tatgcatttc
    13021 gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc
    13081 cagagaactg gcaacacgcg caaaatcggc tggcagactg actacaaacg ctgccaccgc
    13141 aaaccgggat tattgccgtt actgacgccc gagcgcggca tattctgcaa gtatgtgaac
    13201 atctacatat tcccgtaccg gaaaaattat gcgtgattgg catcgataac gaagaactga
    13261 cccgctatct gtcgcgtgtc gccctttctt cggtcgctca gggcgcgcgg caaatgggct
    13321 atcaggcggc aaaactgttg catcgattat tagataaaga agaaatgccg ctacagcgaa
    13381 ttttggtccc accagttcgc gtcattgaac ggcgctcaac agattatcgc tcgctgaccg
    13441 atcccgccgt tattcaggcc atgcattaca ttcgtaatca cgcctgtaaa gggattaaag
    13501 tggatcaggt actggatgcg gtcgggatct cgcgctccaa tcttgagaag cgttttaaag
    13561 aagaggtggg tgaaaccatc catgcaatga ttcatgccga gaagctggag aaagcgcgca
    13621 gtctgctgat ttcaaccacc ttgtcgatca atgagatatc gcaaatgtgc ggttatccat
    13681 cgctgcaata tttctactct gtttttaaaa aagcatatga cacaacgcca aaagagtatc
    13741 gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga attgtcggcg tcacatcagg
    13801 gaactcagca cagccacctg atgcgagcaa aagccttatc cgaccgacaa atcagataag
    13861 gctcgaggaa atgaaattac atatgcgccg cgattaaccg ttggttatcc tggtacattg
    13921 cgaacaggta gttgttataa ctcttccctt tggtcgagta gcccttcagc ttgtgaatca
    13981 tcgctgtggc agtcacttcc tgatccgctt tacgcagctg tgcacgcgat ttacggaacg
    14041 aagagtaagc cgggtgcgta ttcaggttag tgacataggc gctcaccgat tctttgacag
    14101 aactaaactg tgagtaccct ttcactttac ccggcgcatt ggtacaacgt cctttcatgc
    14161 atttcatgcc gaacagattg ttattgttgc gcgccagctt cgacgttccc caaccgcttt
    14221 ctgctgcagc catcgtcgcc accatactgg tagggataat gtctacgcgt tcaagcaagg
    14281 tattccacgg gatttttcgc gtattaccag accacttcac cttgtagcgt ttggcgatgt
    14341 ctttcagacg cgcacgctca gcaggtgccc attggccctg atactgtttt gaaatcagcc
    14401 agttacgttc tgcagtaatg gctgcatttt ggctggtaat gtaaggcatt acggtccgga
    14461 gaaacgcctt tttccttggt gttccggaag ggtattttcg caaatcagga agtgaactac
    14521 tctttgcact attgcgagaa tactcttgtt tactgcttac ctgtttatta ctagctttag
    14581 ttaagtggga cttttgactc gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa
    14641 acaccagagt gagtaacata agaatcatcg ccccatatcg tcgtatggga gtcaaaatca
    14701 tcaggtctcc tggtcggatt taatcattcc aacaccttat atttttcaca aatttgagag
    14761 ttgaatctca aatcatatca aaaatagctg tcaagagcac cccaaggaat agtccaaatc
    14821 tgaaactatg tcacgagtta acgattcaga ttggcgctaa atcgcagaaa atgtgggggt
    14881 tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac ccttgcttct catcctgtgg
    14941 acttaccgct cagggatgag ttttgtctgg cttatcgctg gcaaactgtc tgaaatcgca
    15001 gcaataagga ctcatccacc atgaaactcg ccgcctgttt tctgacactc cttcctggct
    15061 tcgccgttgc cgccagctgg acttctccgg ggttccctgc ctttagcgaa cagggaacgg
    15121 gaacatttgt cagccacgcg cagttgccca aaggtacgcg tccactcacg ctaaattttg
    15181 accaacagtg ctggcagcct gcagatgcga taaaactcaa tcagatgctt tccctgcaac
    15241 cttgtagcaa cacgccgcct caatggcgat tgttcaggga cggcaaatat acgctgcaaa
    15301 tagacacccg ctccggtacg ccaacattga tgatttccat ccagaacgcc gccgaaccgg
    15361 tagcaaacct ggtccgtgaa tgcccgaaat gggatggatt accgctcacg ctggatgtca
    15421 gcgccacttt cccggaagga gccgccgtac gggattatta cagccaacaa attgcgatag
    15481 tgaagaacgg tcaaataacg ttacaacccg ctgctaccag caacggttta ctcctgctgg
    15541 aacgggcaga aactgacaca tccgcccctt tcgactggca taacgccacg gtttactttg
    15601 tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga ccagagttac ggacgtcata
    15661 aagacggtat ggcggaaatt ggcacttttc acggcggcga tttacgcggc ctgaccaaca
    15721 aactggatta cctccagcag ttgggcgtta atgctttatg gataagcgcc ccatttgagc
    15781 aaattcacgg ctgggtcggc ggtggtacaa aaggcgattt cccgcattat gcctaccacg
    15841 gttattacac acaggactgg acgaatcttg atgccaatat gggcaacgaa gccgatctac
    15901 ggacgctggt tgatagcgca catcagcgcg gtattcgtat tctctttgat gtcgtgatga
    15961 accacaccag ctatgccacg ctggcggata tgcaggagta tcagtttggc gcgttatatc
    16021 tttctggtga cgaagtgaaa aaaacgctgg gtgaacgctg gagcgactgg aaacctgccg
    16081 ccggacaaac ctggcatagc tttaacgatt acattaattt cagcgacaaa acaggctggg
    16141 ataaatggtg gggaaaaaac tggatcagaa cggatatcgg cgattacgac aatcctggat
    16201 tcgatgatct cactatgtcg ctggcctttt tgccggatat caaaaccgaa tcaactaccg
    16261 cttctggtct gccggtgttc tataaaaaca aaacggatac tcacgctaaa gtcatcgaag
    16321 gctttacacc tcgcgattac ttaacccact ggttaagtca gtgggtccgc gactatggga
    16381 ttgatggttt tcgggtcgat accgccaaac atgttgagtt gcccgcctgg cagcaactga
    16441 aaaccgaagc cagctccgcg cttcgcgaat ggaaaaaaac taaccccgac aaagcattag
    16501 atgacaaacc tttctggatg accggtgaag cctggggcca cggcgtgatg caaagtgact
    16561 actatcgcca cggcttcgat gcgatgatca atttcgatta tcaggagcag gcggcgaaag
    16621 cagtcgattg tctggcgcag atggatacga cctggcagca aatggcggag aaattgcagg
    16681 gtttcaacgt gttgagctac ctctcgtcgc atgatacccg tctgttccgt gaagggggcg
    16741 acaaagcagc agagttatta ctattagcgc caggcgcggt acaaatcttt tatggcgatg
    16801 aatcctcgcg tccgttcggt cctacaggtt ctgatccgct gcaaggtaca cgttcggata
    16861 tgaactggca ggatgttagc ggtaaatctg ccgccaacgt cgcgcactgg cagaaaatca
    16921 gccagttccg cgcccgccat cccgcaattg gcgcgggcaa acaaacgaca ctttcgctga
    16981 agcagggcta cggctttgtt cgtgagcatg gcgacgataa agtgctggtc atctgggctg
    17041 ggcaacagtg acttttccgg cttcccgttc gtcagtacct cgggaagccg ccaaccagga
    17101 taaaatgtca gccctaatca gcgttgcggg ataaagcacc gctcactctt caacagaccg
    17161 atttgcaccc cagcaaatgt agcgttattg ttaccttcct tgctacagag ttcgacagat
    17221 atcccgctat gacattctcc ctttttggtg acaaatttac ccgccactcc ggcattacgc
    17281 tgctgatgga agatctgaac gacggtttac gcacgcctgg cgcgattatg ctcggcggcg
    17341 gtaatccggc gcagatcccg gaaatgcagg actacttcca gacgctactg accgacatgc
    17401 tggaaagtgg caaagcgact gatgcactgt gtaactacga cggtccacag gggaaaacgg
    17461 agctactcac actgcttgcc ggaatgctgc gcgagaagtt gggttgggat atcgaaccac
    17521 agaatattgc actaacaaac ggcagccaga gcgcgttttt ctacttattt aacctgtttg
    17581 ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt cccgctggca ccggaataca
    17641 ttggctacgc tgacgccgga ctggaagaag atctgtttgt ctctgcgcgt ccgaatattg
    17701 aactgctgcc ggaaggccag tttaaatacc acgtcgattt tgagcatctg catattggcg
    17761 aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc aacaggtaat gtgattactg
    17821 acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca cggcattccg ctggtgattg
    17881 ataacgctta tggcgtcccg ttcccgggta tcatcttcag tgaagggcgc ccgctatgga
    17941 atccgaatat cgtactgtgc atgagtcttt ccaagctggg gctgcctggc tcccgctgcg
    18001 gcattatcat cgccaatgaa aaaatcatca ctgccatcac caatatgaac ggcattatca
    18061 gcctggcacc tggcggtatt ggtccggcga tgatgtgtga aatgattaag cgtaacgatc
    18121 tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta ccagcgtgtt caggaaacta
    18181 tcgccatcat tcgccgctat ttaccggaaa atcgctgcct gattcataaa ccggaaggag
    18241 ccattttcct ctggctatgg tttaaggatt tgcccattac gaccgagcag ctctatcagc
    18301 gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa cttcttcccg ggactggata
    18361 aaccgtggcc gcatacgcat caatgtatgc gcatgaacta cgtaccagag ccggagaaaa
    18421 ttgaggcggg ggtgaagatt ctggcggaag agatagaaag agcctgggct gaaagtcact
    18481 aaaaattgcc tgatgcgcta cgcttatcag gcctacatga tttctgcaat atattgaatt
    18541 tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat ctggcataaa caaagcgcac
    18601 tattccggca ttgagaaacg ccggaaaacg tttcaaacag gctgctgctg tagccgatgc
    18661 cgctgcactt tctcgacatc catgcactgc aacgcctttg tcgggcaggc ttcaacacag
    18721 gccggccccg tctcccgatg ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc
    18781 gaagaaacga cctccatcgc accaaacggg caagccagca tacagctttt acagccaatg
    18841 caacgtgttt gttcaacgaa aatatgccca tgttcgcggc ttatcgcgtc aacagggcag
    18901 acattcgcgc acggtgcatc ttcacactga tgacaggcta ctggcgtggt ccagctgtgg
    18961 tctttaatga cacgaatacg ggaaataaac tcgtctggtg acaacgcagc gcaatcctga
    19021 ttctcctgat gcgacactgc gcaagccact tcacaggtac ggcaaccgat acatttcgtc
    19081 gcatccgcaa taataaaccg gttcatgatc attcctttgc actaaaacag cagagtgcca
    19141 gagagcgcgt ccctactgct ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc
    19201 caaagcgtta tgtgatcgcg cccaggtcat ccctgacagt aaatcccagt tcattagaaa
    19261 tagcctgcgc ggtttcacgc agtggtttca ggagattttt ctctcccacc tgtttcagac
    19321 gtgatgtcga aagcgaaatc gacacggcgt acggcacccg cccatgaata tcaaacaccg
    19381 gaacagcaat acaggagacg ccgagttcgt tttcttctct gtccatcgcc gctccgcttt
    19441 cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta
    19501 acggctggat ctcatgctga tggttttccc agtatgactt cacgtagtcc gggtgaccaa
    19561 acgccatgta gatcttgccc attgcggaac agtagagcgg catatgctgg ccaatatagg
    19621 cacgggttcg cagcatcccg gttgtgggtt ccagcttata aatcaaaata gcgtgatcgt
    19681 cttcgcggct ggagaagtta atggtttcac cagtggcgat gttcagtgcc tcaagatgcg
    19741 gagcggcgat atgaatgata ttcagcgaag acagcgcctt ctgcccgacg gcaataaatt
    19801 tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt cacatagccg caggactgta
    19861 atccctgcaa taagcgatgg acggtactct tatttaaacc agccagctcc gaaagatgcg
    19921 ccaacggaca accgtttgga tagttgctca aaatctcaat cagcatcaac ccgcgaaaca
    19981 gactctggct tccggctgga cgctcttttt cctgcgccat ctcgttctct ttttttccca
    20041 tcacttcttt ccccattttg tcgcgtcctg atggtagcgc aaagtgtgcc gtagttcacg
    20101 atctcgacag ataatttata accacttgat ttttatgtct tttgaaattc atcaatcaga
    20161 ttgctttgtt aaaaagtgat cgatatattt gaaatctagt tccgcatatt gaaattttaa
    20221 gccaaaaaag cgatcaaaaa acaaggaagc ctaggatgaa agtgacattt gagcagttaa
    20281 aagcagcctt taatcgggtc ttaatttcac gcggcgttga cagcgaaacg gctgacgcct
    20341 gtgcagagat gttcgcccgc accaccgaat ccggcgttta ttctcacggc gttaatcgtt
    20401 tccctcgttt cattcaacaa ctggaaaacg gcgatatcat tcctgatgcc caacccaaac
    20461 gtataaccag cctcggcgca attgaacagt gggatgccca gcgttcgatc ggcaacctga
    20521 cggcgaaaaa gatgatggat cgcgccattg aactggctgc cgatcacggt attggtctgg
    20581 tggcacttcg taatgccaac cactggatgc gcggcggcag ctacggctgg caggcggcgg
    20641 aaaaaggcta tattggcatt tgctggacca actccatcgc cgtaatgccg ccgtggggcg
    20701 caaaagagtg tcgcatcggc actaacccgc tgatcgtcgc cattccttcc acgccgatca
    20761 ccatggtcga tatgtcgatg tcgatgttct cttacggcat gttagaagtt aaccgcctgg
    20821 caggccgtca gctcccggtc gatggtggct ttgatgatga gggcaatttg accaaagaac
    20881 ctggcgttat cgagaagaat cgccgcattt tgccgatggg ctactggaaa ggttctggca
    20941 tgtcgattgt gctggatatg atcgctactc tcctttccga cggcgcatcg gttgccgaag
    21001 tcacccagga caacagcgac gaatacggca tttcacaaat ttttattgcc at
//
LOCUS       DAJTRM010000069        20900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRM010000069 DAJTRM010000000
VERSION     DAJTRM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.069"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..434)
                     /locus_tag="OO322_002720"
     CDS             complement(<1..434)
                     /locus_tag="OO322_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5907540.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKI"
     gene            complement(489..1340)
                     /gene="manZ"
                     /locus_tag="OO322_002721"
     CDS             complement(489..1340)
                     /gene="manZ"
                     /locus_tag="OO322_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5907541.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1353..2153)
                     /gene="manY"
                     /locus_tag="OO322_002722"
     CDS             complement(1353..2153)
                     /gene="manY"
                     /locus_tag="OO322_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5907542.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2216..3187)
                     /gene="manX"
                     /locus_tag="OO322_002723"
     CDS             complement(2216..3187)
                     /gene="manX"
                     /locus_tag="OO322_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5907543.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3650..5200
                     /gene="yoaE"
                     /locus_tag="OO322_002724"
     CDS             3650..5200
                     /gene="yoaE"
                     /locus_tag="OO322_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5907544.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5204..6802)
                     /locus_tag="OO322_002725"
     CDS             complement(5204..6802)
                     /locus_tag="OO322_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5907545.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6933..8297)
                     /gene="sdaA"
                     /locus_tag="OO322_002726"
     CDS             complement(6933..8297)
                     /gene="sdaA"
                     /locus_tag="OO322_002726"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5907546.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8481..9059)
                     /locus_tag="OO322_002727"
     CDS             complement(8481..9059)
                     /locus_tag="OO322_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5907547.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9063..10424)
                     /gene="pabB"
                     /locus_tag="OO322_002728"
     CDS             complement(9063..10424)
                     /gene="pabB"
                     /locus_tag="OO322_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5907548.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10498..10677
                     /locus_tag="OO322_002729"
     CDS             10498..10677
                     /locus_tag="OO322_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5907549.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10797..11156)
                     /locus_tag="OO322_002730"
     CDS             complement(10797..11156)
                     /locus_tag="OO322_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5907550.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11518..11862)
                     /locus_tag="OO322_002731"
     CDS             complement(11518..11862)
                     /locus_tag="OO322_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5907551.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11994..13904
                     /locus_tag="OO322_002732"
     CDS             11994..13904
                     /locus_tag="OO322_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5907552.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13962..14657
                     /gene="tsaB"
                     /locus_tag="OO322_002733"
     CDS             13962..14657
                     /gene="tsaB"
                     /locus_tag="OO322_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5907553.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14697..15278
                     /gene="yeaY"
                     /locus_tag="OO322_002734"
     CDS             14697..15278
                     /gene="yeaY"
                     /locus_tag="OO322_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5907554.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15484..17169
                     /gene="fadD"
                     /locus_tag="OO322_002735"
     CDS             15484..17169
                     /gene="fadD"
                     /locus_tag="OO322_002735"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5907555.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17251..18366
                     /gene="rnd"
                     /locus_tag="OO322_002736"
     CDS             17251..18366
                     /gene="rnd"
                     /locus_tag="OO322_002736"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5907556.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18420..19385)
                     /gene="yeaX"
                     /locus_tag="OO322_002737"
     CDS             complement(18420..19385)
                     /gene="yeaX"
                     /locus_tag="OO322_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5907557.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19441..20058)
                     /gene="yeaW"
                     /locus_tag="OO322_002738"
                     /pseudo
     CDS             complement(19441..20058)
                     /gene="yeaW"
                     /locus_tag="OO322_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20400..>20900)
                     /locus_tag="OO322_002739"
     CDS             complement(20400..>20900)
                     /locus_tag="OO322_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5907558.1"
                     /translation="AFAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQ
                     SQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMP
                     EIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQ
                     DVKKAV"
BASE COUNT         5160 a         5194 c         5387 g         5159 t
ORIGIN      
        1 taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg ctgttctaat
       61 tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct atattcaatc
      121 cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat gcggatccag
      181 aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat tagcgcgcca
      241 tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat aacgctatcg
      301 atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg gcggggcatg
      361 atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat cagtaccagg
      421 tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc cggtagtgta
      481 cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat gacgaagaag
      541 ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca agcaaatgtc
      601 agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac ggtggtgaca
      661 cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt gacatgtgtc
      721 cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc ttccgtcagt
      781 ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga gtaaccatac
      841 gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa gaacagcagc
      901 ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa taccggacgt
      961 acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa accgactttg
     1021 ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc ttccagcgcc
     1081 agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa ctccaggtga
     1141 cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg aattgccggt
     1201 accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga accctggaag
     1261 aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt ctcggtggta
     1321 gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc gagatcgttg
     1381 ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct aagttggatg
     1441 tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa gttggtgaat
     1501 gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg catcatgttg
     1561 ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag accattggtc
     1621 accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac agacagcgca
     1681 acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga aacgtggatc
     1741 caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg ctggaaagca
     1801 acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag agggattgcc
     1861 agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt agaaatgata
     1921 gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc cagcgcgatc
     1981 atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac gatacccacc
     2041 agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga tcccatacct
     2101 gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc cattgtactt
     2161 ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca atacgttact
     2221 tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa accttacgga
     2281 cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct ttttcatcaa
     2341 ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca ccgacgttaa
     2401 cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg ttggtaaata
     2461 acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc attttggcaa
     2521 catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt gtcttacgaa
     2581 cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg gtttctttgg
     2641 tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca aggccaataa
     2701 ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt ggtgctgcgg
     2761 cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc acgccttcac
     2821 ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca tcacgggcca
     2881 ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa tgctctttgt
     2941 cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca acgagaaaca
     3001 gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca atcagcgttt
     3061 cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg cctaacagca
     3121 tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata acaatagcaa
     3181 tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat gtatttttga
     3241 atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc cctgcgtttc
     3301 gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt aatttaactt
     3361 tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg caaaggaagg
     3421 taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta acaaccatgg
     3481 agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc atcaggcctt
     3541 ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt gtcgttacgc
     3601 ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca tggaattctt
     3661 aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa ttgtgctggg
     3721 tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa aacaacgcga
     3781 taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc tgctgtcgct
     3841 gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt tctccttctc
     3901 cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag caacaaccga
     3961 actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag gctacgccag
     4021 tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt tggatgcggt
     4081 aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg tagtgattgc
     4141 gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc agcaccccac
     4201 ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg tggcagaagg
     4261 tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct cgatcattat
     4321 cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga ctttgccgct
     4381 gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc aggccaatgt
     4441 tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg aagaagaacg
     4501 gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga tcatgacgcc
     4561 gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc gcgagcaact
     4621 gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg aaatcatcgg
     4681 tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg tggcggcgat
     4741 tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca acctgctggg
     4801 cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg gtgtggtaca
     4861 aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc cggacgctga
     4921 cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg gtacagattt
     4981 gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg atatcgcgac
     5041 ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg atgtgattga
     5101 tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg atctggttcg
     5161 cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt aacgacataa
     5221 tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc agccatttgg
     5281 gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata tgcacgccat
     5341 gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc agttcaatat
     5401 tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc gcgtcagaac
     5461 ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat gtttcaagcc
     5521 aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg tttttacgat
     5581 gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg gtgatttcga
     5641 ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct tttatcagca
     5701 caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga ctactcatcg
     5761 ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt ggcacaatta
     5821 aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc tgacgcggat
     5881 tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca ttaagcaaag
     5941 gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg cgggaaaagc
     6001 tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt agcagcaccg
     6061 ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc ggaccattta
     6121 cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg tcttcaggtt
     6181 gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc accgtcaggc
     6241 tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt aagtcgatgt
     6301 taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat tgaatcaaaa
     6361 ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt tgcggtgcag
     6421 gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg ctggagcaat
     6481 aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt gccgcctgtt
     6541 tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc aagactttgc
     6601 tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga ttggcgaatt
     6661 gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc agggtcgaag
     6721 cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga taggttttaa
     6781 tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac aaacgcctga
     6841 cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg tataaattcg
     6901 cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt gattgccaga
     6961 ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc gtacatcgtt
     7021 tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc catccgtgcg
     7081 gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac ctgaacctgc
     7141 cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc cgccgccacg
     7201 caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc cattgaacag
     7261 gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc gttcatttta
     7321 tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc tgggctgacc
     7381 gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca ggcaccgttg
     7441 gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc cagcgcaaac
     7501 atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct ggaaactaac
     7561 atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag tacaccttcg
     7621 gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc gaaatactcg
     7681 tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc agacagcgaa
     7741 tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg atacggcacg
     7801 ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa accgccgccg
     7861 atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg gatttgcata
     7921 ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc gcgcgggaaa
     7981 tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac gtcgcgaata
     8041 aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag acccataata
     8101 atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata aacgtccacg
     8161 gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac gaactgttta
     8221 cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac cttaaacatg
     8281 tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca gtaacgaact
     8341 aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc acatgaatta
     8401 aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt agtgttttgt
     8461 agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag ctcacgaatt
     8521 atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca tacccgatgt
     8581 gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa tgcctgggcg
     8641 agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg cagatcgggc
     8701 gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac gggcggcagc
     8761 acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc ttcgcgcagc
     8821 gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa tgccacttgt
     8881 ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg ttgcggtcga
     8941 cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg gttaatttgc
     9001 gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg gtattccacg
     9061 tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc ctgatattcc
     9121 gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat ttgtccgtta
     9181 atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa gctcaaatag
     9241 ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat aatttccata
     9301 gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg cagcagatcg
     9361 ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg atgcacggca
     9421 gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac ggcaacacga
     9481 ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc tttcgctgag
     9541 ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg tggtagcgtg
     9601 cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg ctctggcgaa
     9661 aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc gcggttggcc
     9721 tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg aaaacgctgg
     9781 gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg gcgaaatttt
     9841 tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt gaaatcttcc
     9901 tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc attatgactc
     9961 agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta gatacccact
    10021 gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc aaaacggcgg
    10081 cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc ttcgttatgc
    10141 gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg gtcatcagtg
    10201 gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt ggttaaagtg
    10261 caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc atagccggag
    10321 tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc agcggcgtcc
    10381 tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc tgactctact
    10441 ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga gttaacaatg
    10501 tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga gcggatccag
    10561 gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc agaagagctg
    10621 cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga cgagtaacac
    10681 actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga gaaaaaggac
    10741 gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa tttaactcag
    10801 accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt tttaatcaca
    10861 gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt gaacccaggg
    10921 ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg tactcccagt
    10981 tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact tggtgttggc
    11041 gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc aggcatattc
    11101 ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat ttgcatctaa
    11161 tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag gtgttttaaa
    11221 ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc agcccgcacg
    11281 ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa gggcgaacac
    11341 aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga aattataaat
    11401 ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt cgatttatgt
    11461 aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg ataaagctta
    11521 caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac ccgcttgtac
    11581 cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt tattcatcgc
    11641 cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg atttattgct
    11701 gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt gctcaaaggc
    11761 atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt ggattactac
    11821 atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt cctcattata
    11881 cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc acttcaactg
    11941 gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt agtgtgacgg
    12001 acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag ccgcgagaac
    12061 cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag ccgctggtgg
    12121 tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg ctgcgggcga
    12181 aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc tacagccgcg
    12241 atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg ctgaaagggc
    12301 gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg ggcgatctgc
    12361 cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa acagtcgatg
    12421 gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg ctggtcacca
    12481 gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc tttgtggtca
    12541 aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat ctctttctgg
    12601 cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg gacgtcatga
    12661 tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt cagtcactct
    12721 ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc accgaattaa
    12781 aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg caggattttc
    12841 gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta gctaatccgc
    12901 aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat gatgtggcga
    12961 aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc acgttgtatc
    13021 gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac tggtacgaat
    13081 gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat aaattcaaag
    13141 agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg tcggtgaacg
    13201 acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg ttgctattgc
    13261 caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat ctgccgcaaa
    13321 ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc atcgaagcta
    13381 acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat ctggccgagc
    13441 agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt aaaggccaac
    13501 tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt agtttctggg
    13561 aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa ttgccgttta
    13621 cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg cgcggtggcg
    13681 acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag ggggtaggtc
    13741 gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat cggctggtga
    13801 tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc acccgtgaca
    13861 ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt acggagtatg
    13921 gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt ctggctatcg
    13981 ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc aacgctcatt
    14041 ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag gatatcctga
    14101 ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc ggccccggta
    14161 gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt ggcgcggaat
    14221 taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg cgcaaaaacg
    14281 gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac tgggccgaat
    14341 atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta ctcaaacccg
    14401 aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta gggacgggct
    14461 ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat ggcgaagtgt
    14521 tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc gagggtaaaa
    14581 cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca tggaagaaac
    14641 ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt cgcatcatgg
    14701 cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta atgctgagcg
    14761 gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa caagatttag
    14821 ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt ggtggcaaag
    14881 tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc gtaccgctgg
    14941 acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat gccgatgtga
    15001 acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc gggccaatca
    15061 ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg atgcaagtaa
    15121 cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag ccgattgatc
    15181 catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc tggtataatc
    15241 ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt aaagaatgca
    15301 gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa taaatagtga
    15361 cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt taatattatg
    15421 ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt tagaggtgaa
    15481 gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga gatcaaccct
    15541 gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta cgccgatcag
    15601 cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga acgcagtcgc
    15661 gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg cgttgcgttg
    15721 atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg tgccgggata
    15781 atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca gcttaacgat
    15841 agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga aaaagtggtt
    15901 gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct atctacggca
    15961 aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc gaaataccat
    16021 ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat gcagtacgtc
    16081 aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg caccacaggt
    16141 gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga acaggtcaac
    16201 gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc actgccgctg
    16261 tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg tgggcagaac
    16321 ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc gaaatatccg
    16381 tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa taaagagttc
    16441 cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc tgttcagcaa
    16501 gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg ttatggcctg
    16561 accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca tagcggtagc
    16621 atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga caacgaagta
    16681 ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct gggttactgg
    16741 cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac cggcgacatc
    16801 gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga catgattttg
    16861 gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca gcatcctggc
    16921 gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc ggtgaaaatc
    16981 ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt ttgccgccgt
    17041 cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt accgaaatct
    17101 aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa agtggacaat
    17161 aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt ttttgacgcc
    17221 cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc gctggcttct
    17281 ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt tgttcgtacg
    17341 cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca tctggcgcta
    17401 attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg cgatccgtcc
    17461 atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa tgtctttggc
    17521 gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg acgcccgatg
    17581 tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga caagagtgaa
    17641 tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata cgcagcggcg
    17701 gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga ggcctccggc
    17761 tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca ggaagtcgtt
    17821 gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac acgccaactg
    17881 gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg tgatctggcg
    17941 gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat gccgggaagt
    18001 ttaggcgaac tggacagctt gggtttatcc ggtagcgaaa tccgctttca cggtaaaacg
    18061 ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc gcagccgatg
    18121 cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa gtcgctgatt
    18181 actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg tcggcaaatc
    18241 aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc ggagctgatt
    18301 tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca ggaatatccg
    18361 cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc tttggcaaac
    18421 tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa catactttgc
    18481 tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc accttccagt
    18541 attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac tttcgcggct
    18601 ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa ctcttttccg
    18661 gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc aaattgctca
    18721 aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac cgcttcaatt
    18781 agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc caataatcgc
    18841 gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc agcctgcgga
    18901 tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca gtaatgtagc
    18961 tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg ggtgatacca
    19021 ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa caggttatta
    19081 ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa gcgggaaccg
    19141 ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt atgcggtgaa
    19201 ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg cactaggatg
    19261 tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag cgtgaagcgt
    19321 ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat ttgatagtct
    19381 gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc gccgatgtca
    19441 ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat gttcggaaat
    19501 gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac gcgatttcag
    19561 ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat cgcgatacca
    19621 ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa tatcgtagtt
    19681 ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca tccctttgat
    19741 cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg ccgcatccgt
    19801 accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt gcagcgtcca
    19861 gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga aacctagatg
    19921 cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc agttggcagg
    19981 cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc aggcttccag
    20041 cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa accgggagga
    20101 cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag cctgtagcgc
    20161 aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt ttgtttcatt
    20221 gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa aaaacgttca
    20281 aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc tcatgccgtt
    20341 taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt tcatggttgt
    20401 tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc ttcctgttca
    20461 taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga ttcaagcatt
    20521 ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat ggaatcatcg
    20581 gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa ctcaatcgca
    20641 gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt tttcaatgtg
    20701 ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac cattggcatg
    20761 ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat ttgtgctaat
    20821 cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc atcagaaagt
    20881 ccgcacttag ctgcaaacgc
//
LOCUS       DAJTRM010000070        20769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRM010000070 DAJTRM010000000
VERSION     DAJTRM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.070"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..280
                     /locus_tag="OO322_002740"
     CDS             <1..280
                     /locus_tag="OO322_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5907559.1"
                     /translation="AEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALSSTLDSIPL
                     SMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            252..2162
                     /locus_tag="OO322_002741"
     CDS             252..2162
                     /locus_tag="OO322_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5907560.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2163..2366
                     /locus_tag="OO322_002742"
     CDS             2163..2366
                     /locus_tag="OO322_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5907561.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2363..3956
                     /locus_tag="OO322_002743"
                     /pseudo
     CDS             2363..3956
                     /locus_tag="OO322_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3946..5364
                     /locus_tag="OO322_002744"
     CDS             3946..5364
                     /locus_tag="OO322_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5907562.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPLAAMPEMTVEKARPILAASPQANAGPSLRDQIMAL
                     DEAKGAEAQAEKLAACPGMTVENARAVLAAASGRAEPVSASTTAMFEHFMANHSPAAV
                     QGGVPQTSADGDADVKMLMAMP"
     gene            5401..5748
                     /locus_tag="OO322_002745"
     CDS             5401..5748
                     /locus_tag="OO322_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5907563.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5806..6834
                     /locus_tag="OO322_002746"
     CDS             5806..6834
                     /locus_tag="OO322_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5907564.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6886..7272
                     /locus_tag="OO322_002747"
     CDS             6886..7272
                     /locus_tag="OO322_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5907565.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7284..7661
                     /locus_tag="OO322_002748"
     CDS             7284..7661
                     /locus_tag="OO322_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5907566.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7648..8232
                     /locus_tag="OO322_002749"
     CDS             7648..8232
                     /locus_tag="OO322_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5907567.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8229..8630
                     /locus_tag="OO322_002750"
     CDS             8229..8630
                     /locus_tag="OO322_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5907568.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8641..9381
                     /locus_tag="OO322_002751"
     CDS             8641..9381
                     /locus_tag="OO322_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5907569.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9440..9826
                     /locus_tag="OO322_002752"
     CDS             9440..9826
                     /locus_tag="OO322_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5907570.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9835..10164
                     /locus_tag="OO322_002753"
     CDS             9835..10164
                     /locus_tag="OO322_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5907571.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10136..13177
                     /locus_tag="OO322_002754"
     CDS             10136..13177
                     /locus_tag="OO322_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5907572.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13177..13506
                     /locus_tag="OO322_002755"
     CDS             13177..13506
                     /locus_tag="OO322_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5907573.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13506..14204
                     /locus_tag="OO322_002756"
     CDS             13506..14204
                     /locus_tag="OO322_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5907574.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14209..14952
                     /locus_tag="OO322_002757"
     CDS             14209..14952
                     /locus_tag="OO322_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5907575.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14889..15491
                     /locus_tag="OO322_002758"
     CDS             14889..15491
                     /locus_tag="OO322_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5907576.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15552..19031
                     /locus_tag="OO322_002759"
     CDS             15552..19031
                     /locus_tag="OO322_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5907577.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            19099..19698
                     /locus_tag="OO322_002760"
     CDS             19099..19698
                     /locus_tag="OO322_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5907578.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19762..>20769
                     /locus_tag="OO322_002761"
     CDS             19762..>20769
                     /locus_tag="OO322_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5907579.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKTANHLSETAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NG"
BASE COUNT         4855 a         4926 c         6546 g         4442 t
ORIGIN      
        1 cgcagaacag gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga
       61 taccggattt tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga
      121 ctccattcct ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca
      181 tctgaaaatc cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc
      241 ggatttactc gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta
      301 tcgcactgca gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa
      361 attattacct gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc
      421 aggtcgccat catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat
      481 ctgcccgtgt tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca
      541 aatcccgcaa cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt
      601 ctcacgtgga agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg
      661 gccggaaaca ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct
      721 ggtgtttggg tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct
      781 atgacgaact ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg
      841 gcgataaacg tattgaaggt tcggtatggc caaaatccat acgcggttca acgccaaaaa
      901 ttaaaggctc ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt
      961 atgtcccttg ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt
     1021 cgtttggcct gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata
     1081 atggctgcgt gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca
     1141 ataccgggat gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc
     1201 cgccgccgcg ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg
     1261 tacagatagt ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg
     1321 tgaacaccac gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg
     1381 tactgatgga taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga
     1441 cggcgggcat tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg
     1501 gagaggaagc ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga
     1561 cgctgttacg tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa
     1621 tgaccatttc ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga
     1681 ggtcaaaaaa acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca
     1741 agccggtgat caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg
     1801 ggacggatac cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga
     1861 atgaggccac atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag
     1921 aggcacaaca actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc
     1981 tgttgtggga taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg
     2041 cggcattacg tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg
     2101 caaggatgca actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt
     2161 gaatggttac agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg
     2221 gtaaacgggt tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc
     2281 tgaatgagct taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc
     2341 gccgaccgct gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt
     2401 tccgcttcgt gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc
     2461 agagtggtta ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg
     2521 gaatgcccgg gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct
     2581 gcataaggat catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg
     2641 gctggggatg cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc
     2701 ggaatacgca gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga
     2761 atttatccgt gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt
     2821 ctgggatacg gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg
     2881 ggtggacacg ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga
     2941 tcggtatggg cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc
     3001 cggacgctgg gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat
     3061 ttttgagccg gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga
     3121 gcggatgaag atgctggact ccctgcaggc cacgcagctt cagtcggcca ttgtgaaggc
     3181 gatgtatgca gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc
     3241 gggggcaccg caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg
     3301 ctggtatgac acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg
     3361 gcgatgcgct gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac
     3421 aggcgctgct gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg
     3481 attactcgaa ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt
     3541 ttatggggcg gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc
     3601 tggaagaggc gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc
     3661 aggcgcgttc agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg
     3721 ggctcaagga ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga
     3781 aagaactggc actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat
     3841 ccgcagagcg cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc
     3901 agcagatagc ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt
     3961 tcacatatta tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg
     4021 gttttctttt gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag
     4081 caggtacagc ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc
     4141 aaacgacctg cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc
     4201 acgctggtac accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc
     4261 attgtcgcct gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac
     4321 attgacagtt ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt
     4381 ctccggcagc tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg
     4441 ttactggcat cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt
     4501 ggggtgatga tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc
     4561 acgctgattt acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca
     4621 aaggttcgcc aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa
     4681 gtggcgatgt ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt
     4741 gaaggtcagt ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc
     4801 atcagcgtga tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta
     4861 actgcaacgg atgctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag
     4921 gaagcgaaag gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt
     4981 gagcaggccc gggcgattct ggccgtggcg gcaccgctgg cggcgatgcc ggagatgacg
     5041 gtggaaaaag cccgtccgat tctggctgct tcaccgcagg cgaatgccgg accctcactt
     5101 cgtgatcaga ttatggccct ggatgaggca aaaggggcag aggcgcaggc tgaaaaactg
     5161 gcggcctgtc cggggatgac cgtggagaat gcccgggctg tactggcggc agcctcaggc
     5221 agggcagaac cggtttctgc atccacaacc gccatgtttg aacatttcat ggcgaaccat
     5281 tcaccggcag cggtacaggg tggcgtgcca cagacgtcag cagacggtga tgcggacgtg
     5341 aaaatgctca tggccatgcc atgaagtcag tgctgaccat caatatgagg tttttacaat
     5401 atggtaacga aaaccatcac tgaacagcgt gcggaagtac gtatttttgc cggtaatgat
     5461 ccggcccata ccgccacagg ctgcagcggg atttcctcgg caacaccggc tctgacgccc
     5521 ctgatgctgg atgaggatac cgggaaactg gtggtctggg acggacagaa ggcaggccat
     5581 gtggttggca tactggtact gccgcttgaa ggtacagaga cggtgctgac gtattacaag
     5641 tcggggacct ttgcgacgga ggccatccac tggcctgaga gagtggatac acacaaaaag
     5701 gcgaatgcct ttgccggcag tgccctgagt cacgcagcgc tgccgtaaca ttttatcagg
     5761 tcactgcggt agcctgactg atttctgaat gaaaggaact gatttatggg attgtttacg
     5821 acccgccagt tactcggtta taccgaacaa aaagttaaat ttcgtgcgct gtttctggag
     5881 ctgtttttcc gccgtacggt gaatttccat accgaagagg taatgctgga caaaattacc
     5941 ggaaaaacgc cggtggcggc ctatgtctcc ccggttgttg aaggaaaggt gttgcgtcac
     6001 cgtggcggtg aaactcgtgt actgcgcccg ggctacgtta agccgaaaca cgaatttaat
     6061 taccagcagg cggtggagcg tcttcccggt gaagatccgg cccagctgaa cggcccggct
     6121 taccgtcgcc tgcgtatcat caccgataac ctcaaacagg aagagcacgc cattgtccag
     6181 gtggaagaga tgcaggcggt gaatgccgtg ctgtatggca aatacaccat ggaaggagag
     6241 cagtttgaaa ctgttgaggt cgattttggc cggtcggtgg gaaataacat tacgcaggca
     6301 ggagggacgg agtggagcgc tcaggaccgc gatacctttg atccgacgca tgatctggat
     6361 gcgtactgtg attttgcttc cggcaccatc aatattgcca ttatggatgg taaggtctgg
     6421 cgtctgctga atggctttaa gctgttccgc gaaaaactgg atacccgtcg tggctcaaat
     6481 tcacagctgg aaacggcagt gaaagacctg ggggcggtgg tgtccttcaa agggtattat
     6541 ggtgatctgg caatcgtggt ggcaaaaacg tcctatgtgg cagaggacgg taccgaaaaa
     6601 cgttatctgc cggacggcac actggtactg ggaaatacgg ccgcagaggg tattcgttgc
     6661 tacggtgcca ttcaggatgc gcaggcattg tctgaaggcg tggtggcgtc ttcccgttat
     6721 ccgaaacact ggctgaccgt gggtgatccg gcgcgtgaat tcaccatgac gcagtccgcg
     6781 ccgctgatgg tgctgccgga tccggatgag tttgtggtgg tgcaggtgaa ataatccgtg
     6841 agcgggagct aaatgctccc gtttttcatt caatcaggag cgggtatggc tacgaaagaa
     6901 gaaaacctga aacgtctttg tgagctggct gagaggctgg ggcgtgaacc ggatgtctcc
     6961 ggtagtgcgg cagatattgc gcagcgagtg gcggaactgg aggaagagct tggagatgcg
     7021 ggggaggtcg cagagccgga tacgtactca ccatcgaaag atgatccgac cgcgcataaa
     7081 ggcgagtcag ttcctgaaca gccaggcatt gttaccggag acgaaacggg gcaggttacg
     7141 gtggtggcac tggcgacact gcatacaaaa tgtaatggtt cagtgatatt tgtttcaccg
     7201 ggaacgtcgt ttcgtgtttc tgctgaggtg gctgccagca tggcggcagg tggtctggca
     7261 aaaaggcagt aacaggagat gtcgtggacg gtttcgataa tctgtttgat gctgcgcttg
     7321 ccggtgtcaa tgaagtcatt ctccgtgata tggggatcag cgccgtcatt acgtccggag
     7381 aacttgaggg gacacacctg acaggggttt ttgatgatcc cgaaagtatt tcttttgttg
     7441 ccggggggat ccggcttgag gattcctcac cgtgcctgtt tgtgaaaacg gcagatatat
     7501 cgcagttacg ccgtcaggat acgttaacta tcggggatga ttctttcttt gtggatcgta
     7561 ttactccgga tgacggggga tgctgttata tccggttaag gcggggcagt cctgccccac
     7621 agaataatgc cagaatgagg cgatatgatg aagggacttg agaatgccat ccgcaatctg
     7681 aacagcctcg atacccgcat ggtgccacag accagcgcat gggcggtaaa ccgtgtggcg
     7741 gcaaagattg tttctgtcgc cacacggcag gttgcgcaga ataccgttgc cggggataac
     7801 caggtgaaag gtattcccct gaaaacggtc agggagcgtg tcaggctgct taaagccagc
     7861 ccctcaggaa aaatgtatgc ccgcatgcgt gttaaccggg gtaacctgcc cgccattaag
     7921 ctgggtaccg cacaggtcag actggcccgc tcccggcatg gcagcaactc acgtcatcgt
     7981 ggcagcgtac tgaaagtggg gaaatacctt ttccgggatg cgtttattca gcaactggcg
     8041 aatggtcgct ggcatgtgat gcggcgtatt gacggcaaaa atcgttaccc cattgatgtg
     8101 gtgaaaatcc cgatgtccgg accgctgaca caggcatttg aagatgcccg cgaccgcatc
     8161 attgctgcgg aaatgccgaa acagctgggg tatgccctga aacaacaact gaggttacat
     8221 ctgagtaaat gaaccgacac acacaaatcc gtcagagcgt gctggcacgc cttcgggaac
     8281 agtgtggaga cagcgccacg ttttttgacg ggcttccggc atttattgat gcgcaggaac
     8341 tgcccgccgt ggcggtgtgg ctgagtgatg cgcagtacac cggaaaaatg acggatgagg
     8401 acgactggca ggctgttctg catattgccg ttttcgtccg tgcacaggcg ccggattctg
     8461 agctggatac gtggatggag aacaccatat ttccggccat gaaggatatt ccggtgcttt
     8521 ccggactcat cgacaccatg atcccactcg gttttaacta tcaacgtgat aatgagatgg
     8581 ccacctgggc gatggcggaa atcacgtacc agatcacgta cacgaattaa ggaggtggta
     8641 atgaccacac caaatccact ggcaaagacg aaaggtgcgg ggacgacgtt ctggatgtat
     8701 accggcaacg gcgatgcgtt tgcgaaccct ttgtcggaca ctgactggct gcgtcttgcg
     8761 atggtgaagg atctgcaacc tggcgaaatg accgctgatg cagaagatga cacttatctc
     8821 gatgatgaag atgcagactg gaaaacgaca acccaggggc agaaatccgt tggtgatact
     8881 tcggcgacgc tggcctggcg tccgggtgac agcgggcaga aaaaactggt tcagttgttc
     8941 gactccggtg aagtctgcgc gtttcgtatc aaatatccca acggcactgt tgatgttttc
     9001 cgcggctggc tgagctcact gggtaaaacc attgcctcaa aagacgtgat gacccgcact
     9061 gtgaaaatca gcggtgtggg gcgtccgtat ctggcagagg aaggcactga aaccgtgggt
     9121 gttaccggac tgacggtggc accggcatct gtcagtgtca aagcgggagc aaccaccaca
     9181 ctgaccttta cagtaaaacc tgacggggcc agtgacaaag cgatcagtgt gcattcgtca
     9241 gatcctcaga ctgcttcggt gaccctgagc gggcttgtgg ccacggtgaa aggcgtgaag
     9301 cagggcagtg tcagcatcgt gggcatgacc tctgacggag agtttgtggc agtggctgcg
     9361 gtgaccgtca gcgcaccata acaggacgat actcatcatt gccccggtta tccggggctt
     9421 tttgcatccg gagaacatga tgtttctgaa acaggatacg tttaattatg aaaaacagtc
     9481 cgtggtgctc agtgagctgt ccgggctgca gagaattgaa tatctgacgt ttgttcagca
     9541 gcgaacggca aagtttgatg cacaggaggg agaactgccg gaggctgaac gacagattgc
     9601 ttttctgcgt atgggaatgg atatcaatgc ctggctggtt tcccgctcac tgtggaatgc
     9661 tgagcagtct caggatgttg agacgctttg cgcatccatt atgacaacat ggtcgtatga
     9721 tgcgctgggc gcgggggcgg agagggttct gtcgctgagc ggtatgggga ccattgagaa
     9781 tgccggggat gatgatcatg aggcgctgac gccggaaaag tcctgacgcg ggaaatgcag
     9841 tttgtcatgc ggcttgcccg ggagttccgg cgggcagact ggcggcggat gctgtcggat
     9901 atgtcggcca ctgagcttgg tgagtggggc gattatttcc ggatgcagag cttcagtgat
     9961 gtgtggatgg atgcgcagtt tgcctcgctg aaggcattga tcgtgagaat ggtgtccggc
    10021 agcagcgatg ctgcggtggc tgatttcagc cttttaccgg aagagaacgg gataccggag
    10081 cgaacggacg aagaactgat gcatcttggg gaaggtattt ccggaggtgt gcgttatgga
    10141 ccagatagcc aacctggtca ttgatttggg gattgatgcg gcagagttta aaaatgaaat
    10201 tccccgtatc aaaaaccttc tgaatggtgc agccagcgat gcagaacggt cttctgcccg
    10261 tatgcagcgt tttatggagc gtcagactca ggcggcccgg cagacaatgc aggcggcttc
    10321 ttcggctgca acagcagcat cagcccatgc gcagacggtg gagaagaatg cacgggctca
    10381 tgaacgcatg gcccgtgagg tggaacaaac ccgtctgcgc gtggatgccc tgaatcagaa
    10441 aatgcgcgag gaacaggcgc aggccagggc actggcggag gcgcaggata aagcggctgc
    10501 cgccttttat cgccagattg acagtgtgaa acaggccggt gcggggcttc aggaattaca
    10561 gcgtattcag cagcagatcc gacaggccag aaacagtggc ggggttggtc agcaggatta
    10621 tctggcgctg atttcggaga tcacggcgaa aacccgtgcc ctgacgcagg cagaggaaca
    10681 ggccacccgg cagaaagcgg cgtttatccg ccagcttaaa gagcaggcaa cccgccagaa
    10741 tctgtcgtct tctgagttgc ttcgcgccag ggcggctcag ctgggggtaa gcagtgctgc
    10801 agaagtgtat atccgcaaaa tggagcgggc aggaaaagcc acacattcgc tggggctgaa
    10861 aagtgcggca gcccggcggg agctgggggt gttaatcagt cagatggcgc gaggcaattt
    10921 tggtgcgctg aggggatccg ggataacgct ggctaaccgt gccggatgga caggcgcact
    10981 gatgtcgccg aaaggcatga tgactggcgg cgttattggc ggacttgtcg cggcggtcct
    11041 gggtctgggt aaagcctggc atgacggccg gaaggagggc gagaaattta accgtcagct
    11101 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
    11161 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
    11221 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
    11281 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
    11341 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgaatg ccactcagct
    11401 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
    11461 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
    11521 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
    11581 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
    11641 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
    11701 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
    11761 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
    11821 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
    11881 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
    11941 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
    12001 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
    12061 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
    12121 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
    12181 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
    12241 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
    12301 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
    12361 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
    12421 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc ggagggctga
    12481 ggaggtgctg aaggggagcc tgaagcgaca actgaatgaa aacaaacgct actggcagga
    12541 actggaagtg gcgcagggcg actggaaaaa cggtgccatg cgggcgtttc agaattttac
    12601 ggcggatgcg gataatgcgg cgggaacggc agaacagatg tttacagtgg cattcagcag
    12661 tgccggtaat gcactggcga cattctgtac caccggtaag ctgaatttta aatccttcac
    12721 ctcttccctg ttgtcagata tggccagaat tatggcacag atggccatga tgcaggcggt
    12781 aaagggcgtc ggttctttat tcggcttcac gactaatgct gatggcggtg tttaccagtc
    12841 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
    12901 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
    12961 tgctgatggt aagctggggg ttgtggcgga tactggtggt tcaggcatgg tgatgtttgc
    13021 cccgcagtac aacattgaga tcaataacga cggcacgaac gggcagatag gtccggctgc
    13081 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
    13141 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
    13201 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
    13261 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgacgata
    13321 cgtgttcgta aaggtgaaca cccatcttta aaagcctttc tggaacggca cggtggcgtc
    13381 cgcgcatttc agtggacgcc accttatgac tggaaactga tcagggtggt ttgtcgtaaa
    13441 tggtcggcaa gcgtgggggc gttatgggtg actgtaacgg ccgattttga acaggtggtg
    13501 aactgatgca ggatatccgg caggaaacac tgaatgaatg cacccgtgcg gagcagtcag
    13561 cccgggtgga gctctgggaa atcgatctga cagaggtcgg tggtgagcgt tattttttct
    13621 gtaatgagca gaacgaaaaa ggtgagccgg tcacctggca ggggcggcag tatcaggcat
    13681 accccattca ggggtcgggg tttgaactga acggcagggg ctgtgctgcc cgtccgacac
    13741 tgacggtctc taacctgcac ggcatggtca ccgggatggc ggaagatctg cagagtctgg
    13801 tcggcggaac ggtggtccgg cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg
    13861 tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg cgcatcgagc
    13921 agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca acggagacgg
    13981 atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg acttaccgtt
    14041 ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa ccaacaacgg
    14101 atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc aacaataccg
    14161 gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat gacacagaca
    14221 gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg tggcttcgtg
    14281 gtgagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg tgagccggag
    14341 gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg tgagattgtg
    14401 gcactggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga ccggcggctg
    14461 caggtgcaga gtgatttgcc gtggtggctg gtctgccggg ggacgattca caagttccgc
    14521 tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg ttacacgctg
    14581 ttccgggatg cttatcatct ggcggggatt gagatgccgg attttcatcg cggggatgac
    14641 tggtggcgtc acggtcagaa tctctatctg gataatctgg aggccacagg gctgtatcag
    14701 gtgccgttgt catcagcaca accgggcgat gtgctgctgt gctgttttgg ttcatcggtg
    14761 ccgaatcatg ccgccattta ctgcggcgat ggtgagctgc tgcaccatat tcctgaacaa
    14821 ctgagtaaac gagagaggta taccgacaaa tggcagcgac gcacacactc cctctggcgt
    14881 caccgggaat ggcacgcatc tgcctttacg gggatttgca acgatttggc cgccgcatcg
    14941 accttcgtgt gaaaacgggg gctgaagcca tccgcgcact ggccacacag ctcccggcgt
    15001 ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt gatgcaggtg
    15061 aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg atccacatag
    15121 taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg ggggcagctg
    15181 ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat ctgctgtttt
    15241 ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg aaagccagaa
    15301 ctccccgtac acagacaacg gataacggca aacagaacac ctgtttctcc tcactggata
    15361 acatggttgc ccagggcaat gttctgcctg ttctgtacgg tgaaatgcgc gtggggtcgc
    15421 gggtggtatc tcaggagatc agcacggcag acgaagggga tggtggtcag gttgtggtga
    15481 ttggtcgctg atgcaaaatg tttcatgtga aaccgcctgc gggcggtttt gtcgtttatg
    15541 gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
    15601 acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg ccggttgacg
    15661 gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg gatagcgagg
    15721 ggaagacaaa cttttccggt gttacggtgg tgttccgcgc cggcgagcag gagcagacac
    15781 cgccggaggg gtttgaatct tccggctcag agactgtgct gggtacggaa gtgaaatacg
    15841 acacgccgat cacccggacc atcacgtcgg caaacattga tcgtctgcgc ctgaccttcg
    15901 gtgtgcaggc actggtggaa accacctcaa agggggaccg gaatccgtcg gaagtccgcc
    15961 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
    16021 gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt
    16081 ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg cagaacaaaa
    16141 cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcgc
    16201 tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt cgcaattatc
    16261 atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg cggcaataca
    16321 gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc tggtgtctgt
    16381 gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg gcagatgtgg
    16441 acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgccg gacggatttg
    16501 gcggcacgga gccgcgcatc acctgtaacg cttacctgac cacacagcgt aaggcgtggg
    16561 atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
    16621 tgacgttcgt gcaggaccga ccgtcggata aagtgtggac ctataaccgc agtaatgtgg
    16681 tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag gaccgccata
    16741 atgtcgttga ggtgaactgg attgatccgg acaacggtca tgagacggcg acagagcttg
    16801 tggaggacac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    16861 gctgtaccag ccggggacag gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc
    16921 tggaaacgca gaccgtggac ttcagcgtcg gcgcagaagg gcttcgccat gtaccgggtg
    16981 atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt cgcgtactgg
    17041 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccgtcctccg
    17101 gtaccacgct gataagcctg gttgacggaa atggcaatcc ggtcagcgtg gaggttcagt
    17161 ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt gcagaataca
    17221 gcgtgtgggg gctgaagctg ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc
    17281 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagagg
    17341 ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg aatggtgtca
    17401 cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg gaatatcagg
    17461 tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg cttcgcctga
    17521 ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg acggaaacca
    17581 cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg gcggtaaatg
    17641 cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca ccggcagcgc
    17701 ctgtcactat tgaactgata ccggggtatt ttcagataac ggtggtcccg aaactggctg
    17761 tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt gcggatatca
    17821 ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata gccgccagta
    17881 tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac accgttggca
    17941 aatcggcatt tgtggaggcc gtcggtcggg cgagcgatga tgcggaaggt tatctgagtt
    18001 tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg cagattgata
    18061 acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat gtcagcaatg
    18121 aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc cagcagatac
    18181 agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg aagctgcaac
    18241 agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag aatacgccag
    18301 caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt aatcctgcga
    18361 atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg aacgacgtgt
    18421 tcctgaaacg cctgacggct ccgaccatta ccagcggcgg taatcctccg gcattttccc
    18481 tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac gtgaacgcga
    18541 actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg ggaaaattgt
    18601 ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc ccacgggact
    18661 cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat gacgatcagc
    18721 cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa catgagcgga
    18781 agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt
    18841 ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat atgcctgccg
    18901 gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat ggctggtatc
    18961 ccacagcaag tatcagcgat ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca
    19021 gtatcagctg aattttataa cccagatacg ggcaccagaa atggtgcctt ttttattgca
    19081 gaaaagcgag aggtaattat gcgtaaactt tatgctgcca ttttgtccgc agccatctgt
    19141 ctggctgtat ccggtgcgcc tgcatgggcg tctgaacatc ggtccacgct gagcgcgggg
    19201 tatcttcatg cctcgacgaa cgctcccggc agtgatgatc tgaacgggat taacgtgaaa
    19261 taccgttatg agtttacgga cacgctgggg ctggtgacgt cattcagcta tgccaacgct
    19321 gaagatgagc aaaaaacgca ttacagtgat acccgctggc atgaggattc cgtgcgtaac
    19381 cgctggttca gcgtgatggc ggggctgtct gtgcgcgtga atgaatggtt cagcgcgtat
    19441 gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta tctccgcgta
    19501 actgacaaca aggggaaaac gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc
    19561 aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt
    19621 gaccttgctt atgaaggttc cggcagtggc gactggcgca ctgacggttt catcgtgggt
    19681 gtcggttata agttctgatt agccaggtaa cacagtgtta tgacagcccg ctggttcagg
    19741 cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agatggggcg
    19801 ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt
    19861 gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt gattgaggct
    19921 gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg
    19981 gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct gaatgcacca
    20041 aaggatggtg atttacgtcc ggaggtgatg aagcgctttg aggaaatggt ggcgcaggcg
    20101 cagcagagtg ccggggctgc agccggaaac gcacagcaga cggcgcagga tgtggcggca
    20161 gccgcaacgg cccgtgatga tgcacaacgt tttgcggaga aagcccgaca ggatgcaacc
    20221 gtcacagctg aggacagaaa ggccactgcg gaagatgtga caagcacagg agcaaatgca
    20281 gccgcagccg gacagagcgc acaggatgcc gcaggttatg cccgcgcagc agagcaggcc
    20341 aaaaatgaca ttgatgctgc gctgaccggc actctgaaaa cggctaacca tctgtctgaa
    20401 accgcagcag caggcgaaaa ggcacaacag aagtcccggg ataatctggg gctgaaaagt
    20461 gcggccacga tggaagcaca gagcgacatt tacgaccgga caaaaggccg tctggcgata
    20521 cccggcgcat tcggctttgg gtgtgctttt ctgcctgaag atgttatccg ttttgacact
    20581 aagagtgatt tcctggcctg ggtaaggaat gcgctgccag gtgaatattc cgttgctggc
    20641 ccctacggca tcatcatacc cgacacacgg tttgaagggg tgctcagcat ccggtggact
    20701 gatgcacgcc ctgagacaac agaaccgcgg tacagagcca aatcccttac tttttacggc
    20761 attaacggc
//
LOCUS       DAJTRM010000071        20704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRM010000071 DAJTRM010000000
VERSION     DAJTRM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.071"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO322_002762"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO322_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5907580.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO322_002763"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO322_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5907581.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO322_002764"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO322_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5907582.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO322_002765"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO322_002765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5907583.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO322_002766"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO322_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5907584.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO322_002767"
     CDS             complement(8217..9110)
                     /locus_tag="OO322_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5907585.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO322_002768"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO322_002768"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5907586.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO322_002769"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO322_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5907587.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO322_002770"
     CDS             complement(10241..11473)
                     /locus_tag="OO322_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5907588.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO322_002771"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO322_002771"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5907589.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO322_002772"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO322_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5907590.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO322_002773"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO322_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO322_002774"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO322_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5907591.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO322_002775"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO322_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5907592.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO322_002776"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO322_002776"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5907593.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO322_002777"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO322_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5907594.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4979 a         5725 c         5029 g         4971 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccga
//
LOCUS       DAJTRM010000072        20629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRM010000072 DAJTRM010000000
VERSION     DAJTRM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.072"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..518
                     /locus_tag="OO322_002778"
     CDS             <1..518
                     /locus_tag="OO322_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5907595.1"
                     /translation="QRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYS
                     LSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRK
                     GNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPI
                     EYRNQTYMPRV"
     gene            620..772
                     /gene="hokA"
                     /locus_tag="OO322_002779"
     CDS             620..772
                     /gene="hokA"
                     /locus_tag="OO322_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5907596.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(959..1171)
                     /gene="cspA"
                     /locus_tag="OO322_002780"
     CDS             complement(959..1171)
                     /gene="cspA"
                     /locus_tag="OO322_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5907597.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1452..1742)
                     /locus_tag="OO322_002781"
                     /pseudo
     CDS             complement(1452..1742)
                     /locus_tag="OO322_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2176..2886
                     /locus_tag="OO322_002782"
     CDS             2176..2886
                     /locus_tag="OO322_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5907598.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2936..3910)
                     /gene="ghrB"
                     /locus_tag="OO322_002783"
     CDS             complement(2936..3910)
                     /gene="ghrB"
                     /locus_tag="OO322_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5907599.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4014..4664)
                     /locus_tag="OO322_002784"
     CDS             complement(4014..4664)
                     /locus_tag="OO322_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5907600.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4827..7025
                     /locus_tag="OO322_002785"
     CDS             4827..7025
                     /locus_tag="OO322_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5907601.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6994..7434)
                     /locus_tag="OO322_002786"
     CDS             complement(6994..7434)
                     /locus_tag="OO322_002786"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5907602.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7431..7994)
                     /gene="tag"
                     /locus_tag="OO322_002787"
     CDS             complement(7431..7994)
                     /gene="tag"
                     /locus_tag="OO322_002787"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5907603.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8152..8850
                     /locus_tag="OO322_002788"
     CDS             8152..8850
                     /locus_tag="OO322_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5907604.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9079..10287
                     /locus_tag="OO322_002789"
     CDS             9079..10287
                     /locus_tag="OO322_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907605.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10611..12302
                     /gene="eptB"
                     /locus_tag="OO322_002790"
     CDS             10611..12302
                     /gene="eptB"
                     /locus_tag="OO322_002790"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5907606.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12394..12470
                     /locus_tag="OO322_002791"
     tRNA            12394..12470
                     /locus_tag="OO322_002791"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12428..12430,aa:Pro,seq:cgg)
     gene            13214..14821
                     /gene="dppA"
                     /locus_tag="OO322_002792"
     CDS             13214..14821
                     /gene="dppA"
                     /locus_tag="OO322_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5907607.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15129..16148
                     /gene="dppB"
                     /locus_tag="OO322_002793"
     CDS             15129..16148
                     /gene="dppB"
                     /locus_tag="OO322_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5907608.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16158..17060
                     /gene="dppC"
                     /locus_tag="OO322_002794"
     CDS             16158..17060
                     /gene="dppC"
                     /locus_tag="OO322_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5907609.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17071..18054
                     /gene="dppD"
                     /locus_tag="OO322_002795"
     CDS             17071..18054
                     /gene="dppD"
                     /locus_tag="OO322_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5907610.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18051..19055
                     /gene="dppF"
                     /locus_tag="OO322_002796"
     CDS             18051..19055
                     /gene="dppF"
                     /locus_tag="OO322_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5907611.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19085..20356)
                     /locus_tag="OO322_002797"
     CDS             complement(19085..20356)
                     /locus_tag="OO322_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5907612.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4985 a         5071 c         5396 g         5177 t
ORIGIN      
        1 tccaaagaga tttcaaggct acgcggccaa acgagaagtg ggttaccgat gttactgaat
       61 ttgcagtcaa tgggcgcaag ctgtatttgt ctccagtaat agatctcttc aacaacgaag
      121 ttatttctta cagcctttcg gaaagaccag tgatgaacat ggttgagaat atgctcgatc
      181 aggcattcaa aaagcttaat cctcacgagc atcctgttct gcactctgac cagggatggc
      241 agtatcgtat gagaagatat caaaatatcc ttaaagaaca tggtattaaa caaagcatgt
      301 ccagaaaagg caattgtctg gataatgctg tggtggagtg tttctttgga accttaaagt
      361 cggagtgttt ttatcttgat gagttcagta atataagcga actgaaggat gctgttacgg
      421 aatatattga atactataac agcagaagaa ttagcctgaa attaaaaggt ctgactccaa
      481 ttgaatatcg gaatcagacc tatatgcctc gtgtataact gtccaacttt ttggggtcag
      541 tacaaagtga ggctacccta tgcctgaaca ccataaggtt agcctcttac tcgttggaaa
      601 tcaacacagg gggctgggaa tgccgcagaa atatagatta ctttctttaa tagtgatttg
      661 tttcacgctt ttatttttca cctggatgat aagagattca ctgtgtgaat tgcatattaa
      721 acaggagagt tatgagctgg cggcgttttt agcctacaaa ttgaaagagt aagagtcttc
      781 gacgggaaat tattcccgcc ttacttacgg cgttgcgcat tctcattgca cccatattta
      841 ttcttcacaa aaataataat agattttatt acgcgatcga ttatttattt cctgaaaaaa
      901 aataaaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta agcagagatt
      961 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
     1021 ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga
     1081 acacatcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
     1141 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc
     1201 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
     1261 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
     1321 gtcaaaccgt tgatgtgcat taagccactc attggcgggt gatgcaacaa ttatttttca
     1381 tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag
     1441 tggataaaag tctatttcat caactgctta cttaatgccg ggttggcttg aatcaaacgc
     1501 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
     1561 gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt cccttttcat
     1621 aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
     1681 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
     1741 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
     1801 agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta tgattcgatt
     1861 cagcctgtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
     1921 cttatcactc ctgttttgta aagatttcgc aaatctaacc ttctgcgaag gcgtaaatct
     1981 ctggctacaa aaaaggcaac ggcactatca ttttttaaca atgaaacaac aaattaacct
     2041 tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc
     2101 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
     2161 gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg
     2221 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
     2281 gcgtttatcg acttcctgca aaatacagtg atgcgtagcg gtgaacgtct accaaccctg
     2341 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
     2401 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt agacagcgtt
     2461 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaat
     2521 ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc cgatgctgcg
     2581 cactctgcgc taaaacagag cgatgacctg aaaccggtat ttgatcaggc attcaccaaa
     2641 gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca aactttcacg
     2701 caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc
     2761 gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa actgattgcg
     2821 ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
     2881 caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc gcagcttagt
     2941 ccgcgacgtg aggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaaat
     3001 tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg
     3061 gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt
     3121 cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgctgcg atcagtgcat
     3181 tttcgtcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
     3241 cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg atcaggcaaa
     3301 cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt
     3361 ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgca
     3421 cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt
     3481 gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttaccc
     3541 gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg
     3601 cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg
     3661 catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca
     3721 ttttttccag caatgcggca tctacattct cgtttgaacc cagtaaacct tcagcttctg
     3781 caaaaattgc tgcattttga acgacggttt gtgggctgag gtttgccacc tggtgaacgg
     3841 tgaaatgcgc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
     3901 acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg
     3961 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc
     4021 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg
     4081 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
     4141 gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg
     4201 tgaccccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
     4261 agcaccattg ccacgccggt cagggtgtta gcgcctgccg gtttcagggg agcgctgctg
     4321 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
     4381 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
     4441 cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga
     4501 tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc cagacctgcg
     4561 ccgatagcag atttgcctgc ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat
     4621 accgccagag cgccactcac tacggcggca agtaagataa acacgtttct tcattgttaa
     4681 tccttaataa cctttttatt ctttgtcacg ggttccgtgg cgggagatta tgccgcgtga
     4741 acatgaagat tattcctggg aatactcgga aatttgtaag taatatttaa ctgctcaata
     4801 catctaacct ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat tccgttctga
     4861 ctgccgccca ctgggggccc atgctggttg aaaccgacgg cgaaaccgtg tttagctcgc
     4921 gtggcgcgtt agccacagga atggaaaact ccttgcagag cgcggttcgc gaccaggttc
     4981 acagcaatac gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg tcaccggaaa
     5041 acccgcaagg cattcgtggg caggatgaat ttgttcgcgt gagttgggat gaggcgctgg
     5101 caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag gcgatcatgc
     5161 cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg ctggtgctgg
     5221 aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg aaaattgcgt
     5281 ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac agtgggaaaa
     5341 agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc gataaaatgg
     5401 agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc gcccatacgc
     5461 tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg
     5521 tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc gaatgggcgg
     5581 cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa
     5641 ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt gagcaaaaac
     5701 actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc ggcggcggtt
     5761 ttggtctttc ttaccatttt gccaatggtg gtgaccccac gcggcgttct gcggtgctct
     5821 cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc cctgttgccc
     5881 gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt atgaaccgac
     5941 atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact catcatcagg
     6001 ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc tctgaatgct
     6061 tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc tcttttgagc
     6121 gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg atgaagcaag
     6181 tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag ttgagtgaac
     6241 gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg caatggctgg
     6301 aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat
     6361 ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc
     6421 ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta aaaaccgcca
     6481 gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg gattgccctg
     6541 ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca gaacagttgc
     6601 aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac agttctctgc
     6661 gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac gatgctcagg
     6721 cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg cagatccttg
     6781 ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac gaaggggcat
     6841 ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga
     6901 ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg acgctggcat
     6961 ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca ccggccagct
     7021 cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc
     7081 ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca gcatcagatg
     7141 gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc tgacggcctt
     7201 cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa aaccgagaag
     7261 cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat cccgcaccag
     7321 cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg ttgtactttc
     7381 cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa tcatggttta
     7441 tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca tgcctgcata
     7501 aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag tgccttagat
     7561 agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc ttgtgtcacc
     7621 tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc gttctgttcc
     7681 atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg atggcggata
     7741 atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc gaccttcacc
     7801 ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac
     7861 gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt actgtcagtt
     7921 tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg actcacccag
     7981 ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc agccgctttt
     8041 cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc gatatccttc
     8101 atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata
     8161 aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat cagtgccttt
     8221 tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca acccggacat
     8281 tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga tattttgtcg
     8341 caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc ggaagaaact
     8401 cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata tgaacaggtt
     8461 acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat gtataacgag
     8521 tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt gagtagctta
     8581 ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat cagctataac
     8641 cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat gacggcgaca
     8701 aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct caatcaaaat
     8761 attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag cgactatctg
     8821 tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa ccattgaata
     8881 acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg tgaataattg
     8941 tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat ttcatgccgt
     9001 ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt tcctttttat
     9061 tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg gctgacactc
     9121 atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag cctgtttaat
     9181 ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc tttcggcttg
     9241 ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga acgttttggc
     9301 gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt cctgaccgcg
     9361 cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg tctggcggat
     9421 ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt
     9481 ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt caaatttatc
     9541 gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt
     9601 gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa acaggaagtg
     9661 aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg
     9721 cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct gtacgtgatt
     9781 ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc cgcagccaat
     9841 gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg cattctgtct
     9901 gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct ggtgggtatg
     9961 gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg
    10021 gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga gttcttcggc
    10081 ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat cggtagcatt
    10141 tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta cgtgattttc
    10201 gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga gcagaaaatg
    10261 ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc ttcagcttcc
    10321 gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt tgggccagtt
    10381 aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg
    10441 taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg tggtctactt
    10501 attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct gccgggcctg
    10561 aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc atgagataca
    10621 tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt ggccttttta
    10681 tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt accgtctgga
    10741 aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc tttttactac
    10801 gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg gtggtgctct
    10861 tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt tatggcatca
    10921 tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt ctgaacttta
    10981 ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat aaccgctgtc
    11041 gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc ctggtggtcg
    11101 tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc cagcagaaga
    11161 aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta gcgaactctt
    11221 atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg gtcgatgaat
    11281 cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag gctccacaaa
    11341 acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg gaccatatgg
    11401 gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa aatctggcgg
    11461 cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc atgtttgtac
    11521 gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac attttcgcgg
    11581 ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa atgtggttct
    11641 acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg gagccacgta
    11701 atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa tcgctagggc
    11761 gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat tttaactaca
    11821 cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt gttgatagcg
    11881 gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat gtggatcact
    11941 ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc tacgcagctg
    12001 accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt gaactggcac
    12061 cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat ctggaaaatc
    12121 cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa gtgccacgcc
    12181 gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca ccggatggtg
    12241 gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca gcggctaact
    12301 aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg gattgacgag
    12361 ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc tggtagcgca
    12421 cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca aattcgaaaa
    12481 gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga acctccgggg
    12541 gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg
    12601 gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac accacacatt
    12661 ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg acagaaacca
    12721 ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg cgctggcatt
    12781 ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc ttttcctcag
    12841 atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat taagctgatt
    12901 gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt tgaagttttt
    12961 ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt
    13021 ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga tccgcactgt
    13081 tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga ccgggcaaaa
    13141 aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca aacatcacaa
    13201 ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc ttggtctcag
    13261 cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt attgctcaga
    13321 aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct atgacgcctc
    13381 ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg aagtgatccc
    13441 gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct tccatctgcg
    13501 taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa tgaacgccga
    13561 tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc
    13621 tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca gcgaagtgaa
    13681 aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc cgttcctcgc
    13741 tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg cgatgatgaa
    13801 agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt tccagttaca
    13861 gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact ggggcaccaa
    13921 accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc gttacgcgaa
    13981 attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca tcgctcgcat
    14041 gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg gctacctctc
    14101 gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac taacctacgc
    14161 ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa
    14221 cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca cctacgatcc
    14281 tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct ccatcgacct
    14341 gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg cggagatgat
    14401 tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg aatggggtga
    14461 gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct ggactggcga
    14521 taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg
    14581 ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc
    14641 taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga
    14701 tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac gcaaagaagt
    14761 caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct ctatcgaata
    14821 attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct ccgcttatca
    14881 ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg gataaggcgt
    14941 taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata cccggtggcg
    15001 ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc cgacaagggc
    15061 gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca ctatagagaa
    15121 tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc acgtttatcg
    15181 gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg gtgatgatca
    15241 tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct gaactcggct
    15301 tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg cacggcgatc
    15361 taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg cgctttcagg
    15421 ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt attccggttg
    15481 gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt ggcctggcgc
    15541 tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg ctggtttcgg
    15601 tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc ctcgatgact
    15661 ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa gacggcaact
    15721 ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact attccgctag
    15781 cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag gattacatcc
    15841 gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat gcgctgcgta
    15901 acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg ctggcggggg
    15961 cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg attgacgcac
    16021 tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg acgatgatta
    16081 tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt attcgtcata
    16141 agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc gcaccggtgc
    16201 cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc gcggtcgtcg
    16261 ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg attgcgcctt
    16321 ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg caggaaggcg
    16381 gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg tcgcggctga
    16441 tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg tcgctgatta
    16501 tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat aacattatca
    16561 tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg gtgctggtgg
    16621 cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt gccttgccgc
    16681 actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg
    16741 cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc ttcccgaact
    16801 gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt ctcgatatgg
    16861 ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg ggcaccatgc
    16921 tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc cctggtctgg
    16981 cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg
    17041 atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga taaattatcg
    17101 gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag ctacagcgta
    17161 aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc ggtcagttca
    17221 ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa actggagttt
    17281 aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt gggtgccgaa
    17341 gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac cgtgggtttc
    17401 cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg ccgtcagcgg
    17461 gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac
    17521 ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc gattgcctgt
    17581 cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac cattcaggcg
    17641 caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct ggtgttaatt
    17701 acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat gtatgcaggt
    17761 caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca cccgtatact
    17821 caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct ggcgtcgttg
    17881 ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa cccgcgctgc
    17941 ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct cgctgatggg
    18001 cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact atgagtacgc
    18061 aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa cattatccgg
    18121 tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc gtttcgttta
    18181 accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt aaatcgaccc
    18241 tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat tatcaggggc
    18301 aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa atccagatcg
    18361 tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa attctcgaag
    18421 agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa gccctgtcga
    18481 tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac atgttttccg
    18541 gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg gatgtggtga
    18601 ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg ctgaatctga
    18661 tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac gacctgtcgg
    18721 ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc gtggagaaag
    18781 gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg ctgctctccg
    18841 cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc ggtgaactgc
    18901 caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt cggcgcttcg
    18961 gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg gtagcttgtt
    19021 ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg ccatgcgctc
    19081 cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg taaagcagaa
    19141 tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag atcaggaaaa
    19201 acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag aacaccagca
    19261 ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg cagatcgcca
    19321 gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac agattgagaa
    19381 taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg
    19441 caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc acctgcgcgg
    19501 ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg tggctaattg
    19561 agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata taactaattc
    19621 ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt ttacgatagg
    19681 caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa aatggaatgg
    19741 taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata ttggcgaaat
    19801 tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg accaccacca
    19861 ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct atcagtacgg
    19921 cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg
    19981 ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa atcccgtgga
    20041 taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc aggtaatgac
    20101 tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt aaataaatgt
    20161 cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat acccaaattc
    20221 ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc atgcctatgc
    20281 ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg tgtttcgata
    20341 gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa
    20401 cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc
    20461 aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg catttcacta
    20521 ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa attaagctgg
    20581 aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagca
//
LOCUS       DAJTRM010000073        20536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRM010000073 DAJTRM010000000
VERSION     DAJTRM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.073"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            97..396
                     /locus_tag="OO322_002798"
     CDS             97..396
                     /locus_tag="OO322_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5907613.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="OO322_002799"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="OO322_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5907614.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO322_002800"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO322_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5907615.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="OO322_002801"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="OO322_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5907616.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="OO322_002802"
     CDS             complement(2535..2879)
                     /locus_tag="OO322_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5907617.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="OO322_002803"
     CDS             3031..3261
                     /locus_tag="OO322_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907618.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="OO322_002804"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="OO322_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5907619.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="OO322_002805"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="OO322_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5907620.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="OO322_002806"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="OO322_002806"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5907621.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="OO322_002807"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="OO322_002807"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5907622.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="OO322_002808"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="OO322_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5907623.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="OO322_002809"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="OO322_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5907624.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="OO322_002810"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="OO322_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5907625.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="OO322_002811"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="OO322_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO322_002812"
     CDS             12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO322_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5907626.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="OO322_002813"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="OO322_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5907627.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="OO322_002814"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="OO322_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5907628.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="OO322_002815"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="OO322_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="OO322_002816"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="OO322_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5907629.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO322_002817"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO322_002817"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5907630.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO322_002818"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO322_002818"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5907631.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19828)
                     /locus_tag="OO322_002819"
     CDS             complement(19400..19828)
                     /locus_tag="OO322_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5907632.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO322_002820"
     CDS             complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO322_002820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5907633.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4959 a         5252 c         5126 g         5199 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcatga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatattg gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaagaaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    19621 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    19681 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    19741 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    19801 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    19861 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg
    19921 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    19981 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    20041 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact
    20101 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    20161 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    20221 tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta
    20281 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc
    20341 caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    20401 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    20461 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    20521 gaaatttttt ggagaa
//
LOCUS       DAJTRM010000074        20279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRM010000074 DAJTRM010000000
VERSION     DAJTRM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.074"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO322_002821"
     CDS             complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO322_002821"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5907634.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO322_002822"
     CDS             complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO322_002822"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5907635.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1718..2990)
                     /locus_tag="OO322_002823"
                     /pseudo
     CDS             complement(1718..2990)
                     /locus_tag="OO322_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3173..4378)
                     /locus_tag="OO322_002824"
     CDS             complement(3173..4378)
                     /locus_tag="OO322_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907636.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4478..4834)
                     /locus_tag="OO322_002825"
     CDS             complement(4478..4834)
                     /locus_tag="OO322_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5907637.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4970..5077)
                     /gene="rprA"
                     /locus_tag="OO322_002826"
     ncRNA           complement(4970..5077)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO322_002826"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5263..6375)
                     /gene="ydiK"
                     /locus_tag="OO322_002827"
     CDS             complement(5263..6375)
                     /gene="ydiK"
                     /locus_tag="OO322_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5907638.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6764..9820
                     /locus_tag="OO322_002828"
     CDS             6764..9820
                     /locus_tag="OO322_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5907639.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9817..10227
                     /gene="menI"
                     /locus_tag="OO322_002829"
     CDS             9817..10227
                     /gene="menI"
                     /locus_tag="OO322_002829"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5907640.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10327..10515
                     /locus_tag="OO322_002830"
     CDS             10327..10515
                     /locus_tag="OO322_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5907641.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11063..11431
                     /gene="sufA"
                     /locus_tag="OO322_002831"
     CDS             11063..11431
                     /gene="sufA"
                     /locus_tag="OO322_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5907642.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11440..12927
                     /gene="sufB"
                     /locus_tag="OO322_002832"
     CDS             11440..12927
                     /gene="sufB"
                     /locus_tag="OO322_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5907643.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12937..13683
                     /gene="sufC"
                     /locus_tag="OO322_002833"
     CDS             12937..13683
                     /gene="sufC"
                     /locus_tag="OO322_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5907644.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13658..14929
                     /gene="sufD"
                     /locus_tag="OO322_002834"
     CDS             13658..14929
                     /gene="sufD"
                     /locus_tag="OO322_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5907645.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14926..16146
                     /gene="sufS"
                     /locus_tag="OO322_002835"
     CDS             14926..16146
                     /gene="sufS"
                     /locus_tag="OO322_002835"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5907646.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16159..16575
                     /gene="sufE"
                     /locus_tag="OO322_002836"
     CDS             16159..16575
                     /gene="sufE"
                     /locus_tag="OO322_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5907647.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16724..17728
                     /gene="ldtE"
                     /locus_tag="OO322_002837"
     CDS             16724..17728
                     /gene="ldtE"
                     /locus_tag="OO322_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5907648.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17791..18027)
                     /gene="lpp"
                     /locus_tag="OO322_002838"
     CDS             complement(17791..18027)
                     /gene="lpp"
                     /locus_tag="OO322_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5907649.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18338..19750)
                     /gene="pykF"
                     /locus_tag="OO322_002839"
     CDS             complement(18338..19750)
                     /gene="pykF"
                     /locus_tag="OO322_002839"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5907650.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5311 a         4861 c         5175 g         4932 t
ORIGIN      
        1 tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc ttatgcctga
       61 tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg cgcagaggct
      121 ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag acgagaaatt
      181 acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg ctcctgcatc
      241 tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc
      301 ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat ttcttcggct
      361 tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg cgcgtgggcg
      421 taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc gatcatatca
      481 accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt ggaaatcgcc
      541 tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc tggcatggtt
      601 tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc ggcaaagtgg
      661 tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc ttcggatttc
      721 acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc cgcaccaatg
      781 acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt
      841 caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt gccagtccat
      901 aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat cgttttgcaa
      961 ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac gcattcagtg
     1021 accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt ctcaaggggt
     1081 ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc ttcagcaaag
     1141 gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc gttaacccgc
     1201 tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag tttaatttct
     1261 tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac
     1321 agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg aatatggccc
     1381 gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt gttgatggca
     1441 cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag ttgtttgttc
     1501 ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat tgctccggga
     1561 aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc tttttctaag
     1621 gctttattct gcatttcagg cgataaactg tggcggatag gataagccat caatccaatc
     1681 aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt
     1741 ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga ataataaaaa
     1801 cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa
     1861 aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa
     1921 taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt
     1981 tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga
     2041 aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg
     2101 cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc cggaccattt
     2161 ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga
     2221 tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc
     2281 acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac
     2341 cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc tacgctggcg
     2401 tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag tacaaacaga
     2461 atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat atagctcacc
     2521 agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat gaccgccgaa
     2581 ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc cagcgctgag
     2641 ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg gcaagcggga
     2701 ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc ccgacgacca
     2761 aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa accgataccg
     2821 gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag agatgtcata
     2881 ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta aatacaaaga
     2941 acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat tgttctccga
     3001 cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat
     3061 gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc
     3121 agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg cattacccac
     3181 ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg cgtaagccga
     3241 taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg gcaatacttc
     3301 tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg cccgtactgt
     3361 aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt aacgtcaggc
     3421 caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat attatcacca
     3481 cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata aaggtgtaca
     3541 gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta ataaatacac
     3601 aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg tatgacatgc
     3661 ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac gttgccattg
     3721 agatatagcc atataaggta taactggcta aatcgataat tgaacagcga tgttccgtag
     3781 agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg aacgtacaac
     3841 ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag gaccaaccga
     3901 accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat tgtccgctgg
     3961 aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa gcttccatca
     4021 aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga aagccaaaaa
     4081 cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc atatagcagc
     4141 acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat cctgcaaaaa
     4201 gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca cccgcggcat
     4261 tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg acgcccatac
     4321 catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga tttttcatag
     4381 gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta tccattcatt
     4441 attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc tccagatcgt
     4501 gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa ttgccatcag
     4561 ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg ttgccgatgc
     4621 ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga cgcatagcca
     4681 acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac tgacgccagc
     4741 tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc atggcaaaaa
     4801 tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat gaaataaaca
     4861 ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac agtatcttgt
     4921 gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt taaaaaaaag
     4981 cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac tcaggggatt
     5041 tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag gcatacggac
     5101 tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt tgcgacaggt
     5161 actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa atatcaatta
     5221 gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat tcggtttctc
     5281 gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg ggacttcttc
     5341 cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa taaacagacc
     5401 gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga tcagcggtaa
     5461 atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc caaccacacc
     5521 gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga gccagataat
     5581 cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga tcattaacac
     5641 cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc caagcactgc
     5701 ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct gtgccgccag
     5761 cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc gaatgccttg
     5821 tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca gcgcacaatg
     5881 caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg tggtgccaat
     5941 ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt tgtgccagcc
     6001 tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat cgggtaacgt
     6061 catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga tactgttaac
     6121 cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca ccagaacggc
     6181 gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc aggtggcgat
     6241 aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc acagacatgc
     6301 cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat ccctgggctg
     6361 acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc gacgcgacgt
     6421 gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta acaggccgat
     6481 tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac tcaggcaaca
     6541 cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt tcgttcaccc
     6601 cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc tgcaactcga
     6661 aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc tgaaagaata
     6721 gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc cacagatttc
     6781 cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg agcaacaagg
     6841 ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga ccgacaacag
     6901 tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag atgtggcgct
     6961 gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc cccgcggcgg
     7021 cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata tgtcccgcca
     7081 tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg aggccggggt
     7141 gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg cgccggaact
     7201 ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat ccggtcaggg
     7261 atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg tgttgttggg
     7321 gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc tgggtaaatc
     7381 caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc agcaacgcca
     7441 gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg atctgcgtca
     7501 tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg gttcagaagg
     7561 gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta aagtgcgccg
     7621 tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc cgtttatggt
     7681 tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc tggcgcggga
     7741 agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca aagagatgct
     7801 cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg agcgagttaa
     7861 tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg tgattggctg
     7921 gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca aaaaagccgt
     7981 tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg aggatacctg
     8041 cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc tcgacagcca
     8101 cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg tccgtccggc
     8161 actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg atgacgtggt
     8221 ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag gttttcgcgc
     8281 tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca aagtgaaagc
     8341 ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag aaggtctcga
     8401 tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc agatccccat
     8461 tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt tatgcttcaa
     8521 ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc ggattcattc
     8581 accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg atcgcggcgt
     8641 tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc ggacgttaat
     8701 tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct cacacgaagt
     8761 caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt gccccatcaa
     8821 aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc gttatttacg
     8881 cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga tggcacgcgc
     8941 gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct cggaaaaaca
     9001 tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac aaatggtggg
     9061 gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag agcagaaagc
     9121 gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc aagtggtggc
     9181 ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc cattttcgcc
     9241 aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga cggcgaaaaa
     9301 gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg gcgtcgatcc
     9361 ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg aacgtggcga
     9421 gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac atccagtggc
     9481 tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta ccgctttgcc
     9541 gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac tggaaaatgt
     9601 cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa accatgaaaa
     9661 ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc cgcgtaaccg
     9721 ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag gcacgggggt
     9781 acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg gaaaatcacc
     9841 ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga tattcgcttt
     9901 gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg gacaaagcag
     9961 cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat cggttccgtt
    10021 gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat caatgctaat
    10081 cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt gcatctcggt
    10141 tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt gtgctgttcg
    10201 tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac gcatattgct
    10261 tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc taaaagcgag
    10321 gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt acgccgtgac
    10381 cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga acttgaattt
    10441 gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt cgcctggcgt
    10501 ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc tggattaacc
    10561 tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata ttgttcaaaa
    10621 ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc
    10681 gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg gggtagaaac
    10741 ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct gcactttttt
    10801 acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg
    10861 ctccttagat aataaccatt atctaacaat gagataccta attcttagcg tgcctgttaa
    10921 cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt acggtaaagc
    10981 ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt atacgctgaa
    11041 agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc cacaggattt
    11101 cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg agctggtggc
    11161 aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct gcgcgggctt
    11221 tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga
    11281 cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca cggaagtcga
    11341 tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga
    11401 atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa tactgaagca
    11461 actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg attcttcacc
    11521 cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg
    11581 gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta tcgcgcatgg
    11641 ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat
    11701 tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc gtctgaacct
    11761 ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt
    11821 gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc cattttcgac
    11881 tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat tattttctgt
    11941 tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg
    12001 gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc tgacggtacg
    12061 tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta ttttcgcatt
    12121 aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga agacagctac
    12181 gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt gcacgcggca
    12241 gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg
    12301 ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc
    12361 gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc gattacgtgg
    12421 aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg
    12481 ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac
    12541 accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc
    12601 ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgactca
    12661 atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac
    12721 agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac
    12781 tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa cggtttctgc
    12841 aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc
    12901 atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac
    12961 gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa
    13021 gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc
    13081 gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa agatttgctt
    13141 gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca gtatccggtg
    13201 gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc agtgcgcagc
    13261 tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc
    13321 gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc
    13381 ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt
    13441 cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc cgatggcgtg
    13501 aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca acgcattctc
    13561 gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt gaaatccggc
    13621 gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag
    13681 taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac gctcaccgca
    13741 agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa
    13801 ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca ttgcgggaga
    13861 tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc ggctggtgtt
    13921 tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg gatatgaagt
    13981 gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag tgtttctgca
    14041 tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc
    14101 ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag aggtgaacac
    14161 tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
    14221 ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta tcaatgtcgc
    14281 agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca gtcaccactt
    14341 tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata gtttcctgct
    14401 gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct
    14461 gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc gtacctggct
    14521 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
    14581 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
    14641 tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata caaaaccgca
    14701 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
    14761 tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat
    14821 gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca
    14881 gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtc
    14941 gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt gccgctggct
    15001 tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc cgaggccgag
    15061 ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaggcg
    15121 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
    15181 gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
    15241 ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct
    15301 aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatccct
    15361 ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga tgagaaaact
    15421 cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc actggcggaa
    15481 atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg
    15541 atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt ctccgggcat
    15601 aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt gttgcaggag
    15661 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcact
    15721 acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg gggcatcatt
    15781 ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat agccgagtat
    15841 gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga tctcactctc
    15901 tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca ccacgcctat
    15961 gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca tcactgcgct
    16021 atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct ggccatgtat
    16081 aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca ccgtctgctg
    16141 ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt
    16201 ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg ccagcgtctg
    16261 ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg ccagagtcag
    16321 gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg cgacagcgat
    16381 gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca gatgacgccg
    16441 caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat
    16501 ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
    16561 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg
    16621 tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat aagcaccatc
    16681 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt
    16741 gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta
    16801 tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata cggtgcaaga
    16861 aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa tgttgatcct
    16921 tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa ctattccttc
    16981 acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg cagagctgcg
    17041 cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag gtattggatt
    17101 gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc ctaacccaac
    17161 ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta aattaccgcc
    17221 agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc tcgcgcatgg
    17281 taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt tgcgcgtcag
    17341 ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca gcgtgcggac
    17401 gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac ctaacgggat
    17461 gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg ttcagacaat
    17521 gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa
    17581 gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga gcagtggagc
    17641 aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca
    17701 agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa tggcgcacaa
    17761 tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag ccatgttgtc
    17821 cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc
    17881 gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt cagaagacag
    17941 ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca ggattaccgc
    18001 gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt taatctccat
    18061 gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt gatgggaagg
    18121 cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag ataaaaaaat
    18181 tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat tatatagtga
    18241 aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag cgcccatcag
    18301 ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga acagatgcgg
    18361 tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg tcacctttgt
    18421 gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca tcagtagaag
    18481 tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga tgagccgttt
    18541 tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt actgcgcgag
    18601 cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt tcagcagttt
    18661 caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca ttgttgaact
    18721 cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg atagaaaccg
    18781 cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg tcagtaccgt
    18841 cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg tttttgatca
    18901 tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata catttttcga
    18961 tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc aggtcgccac
    19021 gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg aggccttcct
    19081 ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc aggtgctcac
    19141 ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag tctacgcctt
    19201 tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc agagcaatgg
    19261 aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc agcactttac
    19321 agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca tcgtcaacca
    19381 gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc gcaaccattt
    19441 cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca gctttcagag
    19501 aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg gtatcaagca
    19561 ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga atgcgctgac
    19621 cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg ccagcgtcca
    19681 gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa acaattttgg
    19741 tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact tgctttctgg
    19801 gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc tctaaggata
    19861 ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca acgtgagtgt
    19921 ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa gagtcaattg
    19981 gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt tcaaaaaata
    20041 tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga aaaggttacg
    20101 caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga ggataagtga
    20161 aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa taatttatcg
    20221 cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata tatgctgat
//
LOCUS       DAJTRM010000075        20121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRM010000075 DAJTRM010000000
VERSION     DAJTRM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.075"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..163
                     /gene="raiA"
                     /locus_tag="OO322_002840"
     CDS             <1..163
                     /gene="raiA"
                     /locus_tag="OO322_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5907651.1"
                     /translation="LVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVKDA
                     NFVEEVEEE"
     misc_feature    266..391
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            267..314
                     /gene="pheL"
                     /locus_tag="OO322_002841"
     CDS             267..314
                     /gene="pheL"
                     /locus_tag="OO322_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5907652.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            413..1573
                     /gene="pheA"
                     /locus_tag="OO322_002842"
     CDS             413..1573
                     /gene="pheA"
                     /locus_tag="OO322_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5907653.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1616..2737)
                     /gene="tyrA"
                     /locus_tag="OO322_002843"
     CDS             complement(1616..2737)
                     /gene="tyrA"
                     /locus_tag="OO322_002843"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5907654.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2748..3818)
                     /gene="aroF"
                     /locus_tag="OO322_002844"
     CDS             complement(2748..3818)
                     /gene="aroF"
                     /locus_tag="OO322_002844"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5907655.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4028..4393
                     /locus_tag="OO322_002845"
     CDS             4028..4393
                     /locus_tag="OO322_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5907656.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4543..5061
                     /locus_tag="OO322_002846"
     CDS             4543..5061
                     /locus_tag="OO322_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5907657.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5051..6276
                     /gene="dgcN"
                     /locus_tag="OO322_002847"
                     /pseudo
     CDS             5051..6276
                     /gene="dgcN"
                     /locus_tag="OO322_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6292..6774
                     /locus_tag="OO322_002848"
     CDS             6292..6774
                     /locus_tag="OO322_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5907658.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(6851..7198)
                     /gene="rplS"
                     /locus_tag="OO322_002849"
     CDS             complement(6851..7198)
                     /gene="rplS"
                     /locus_tag="OO322_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5907659.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7240..8007)
                     /gene="trmD"
                     /locus_tag="OO322_002850"
     CDS             complement(7240..8007)
                     /gene="trmD"
                     /locus_tag="OO322_002850"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5907660.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8038..8586)
                     /gene="rimM"
                     /locus_tag="OO322_002851"
     CDS             complement(8038..8586)
                     /gene="rimM"
                     /locus_tag="OO322_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5907661.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8605..8853)
                     /gene="rpsP"
                     /locus_tag="OO322_002852"
     CDS             complement(8605..8853)
                     /gene="rpsP"
                     /locus_tag="OO322_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5907662.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(8990..10351)
                     /gene="ffh"
                     /locus_tag="OO322_002853"
     CDS             complement(8990..10351)
                     /gene="ffh"
                     /locus_tag="OO322_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5907663.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10518..11309
                     /locus_tag="OO322_002854"
     CDS             10518..11309
                     /locus_tag="OO322_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5907664.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11330..12616
                     /locus_tag="OO322_002855"
     CDS             11330..12616
                     /locus_tag="OO322_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5907665.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12671..13264)
                     /gene="grpE"
                     /locus_tag="OO322_002856"
     CDS             complement(12671..13264)
                     /gene="grpE"
                     /locus_tag="OO322_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5907666.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13387..14265
                     /gene="nadK"
                     /locus_tag="OO322_002857"
     CDS             13387..14265
                     /gene="nadK"
                     /locus_tag="OO322_002857"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5907667.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14351..16012
                     /gene="recN"
                     /locus_tag="OO322_002858"
     CDS             14351..16012
                     /gene="recN"
                     /locus_tag="OO322_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5907668.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16161..16502
                     /gene="bamE"
                     /locus_tag="OO322_002859"
     CDS             16161..16502
                     /gene="bamE"
                     /locus_tag="OO322_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5907669.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16564..16854)
                     /locus_tag="OO322_002860"
     CDS             complement(16564..16854)
                     /locus_tag="OO322_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5907670.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(16844..17281)
                     /gene="ratA"
                     /locus_tag="OO322_002861"
     CDS             complement(16844..17281)
                     /gene="ratA"
                     /locus_tag="OO322_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5907671.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17452..17934
                     /gene="smpB"
                     /locus_tag="OO322_002862"
     CDS             17452..17934
                     /gene="smpB"
                     /locus_tag="OO322_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5907672.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18149..18511
                     /gene="ssrA"
                     /locus_tag="OO322_002863"
     tmRNA           18149..18511
                     /gene="ssrA"
                     /locus_tag="OO322_002863"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18716..19957
                     /locus_tag="OO322_002864"
     CDS             18716..19957
                     /locus_tag="OO322_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5907673.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5163 a         5267 c         4745 g         4946 t
ORIGIN      
        1 tctggttgcc agtggtaaac atgaagatat gtacaccgca attaacgaat tgatcaacaa
       61 gctggaacgg cagctcaata aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc
      121 ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgt
      181 atcgccaacg cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg
      241 tactaaagtc acttaaggaa acaaacatga aacacatacc gtttttcttc gcattctttt
      301 ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat
      361 aaggcctccc aaatcggggg gcctttttta ttgataacaa aaaggcaaca ctatgacatc
      421 ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      481 gttactggca gaacggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      541 tcgcccggta cgtgatattg atcgtgaacg cgatttgctg gaaagattaa ttacgctcgg
      601 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
      661 ttccgtatta actcagcagg ctttgctcca acaacatctc aataaaatta atccgcactc
      721 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
      781 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
      841 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
      901 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
      961 gatgacgtta actatcgacc attgtttgtt ggtctccggc actactgatt tatccaccat
     1021 caatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1081 tccgcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1141 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1201 gcaggtactg gagcgtattg aagcaaatca gcgacaaaac ttcacccgat ttgtggtgtt
     1261 ggcgcgtaaa gccattaacg tgtctgatca ggttccggcg aaaaccacgt tgttaatggc
     1321 gaccgggcaa caagccggtg cgttggttga agcgttgctg gtactgcgca accacaatct
     1381 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgttcta
     1441 tctggatatt caggctaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1501 ggaaatcacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1561 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     1621 ggcgattgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     1681 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     1741 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     1801 tacgctctga cgacataatg atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     1861 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     1921 gaacattttc ttctgccagg tgcagcccgt aagcaaaagt agcaaagtgg cgcagagcct
     1981 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcaac cgagcgcccc
     2041 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2101 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2161 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2221 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2281 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2341 agtcatgttg ctccagaatc cgcacctgat aacccgatag tgtcagcatc ttctcgaaca
     2401 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2461 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2521 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     2581 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc accttgccca
     2641 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     2701 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     2761 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     2821 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     2881 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgatta
     2941 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3001 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3061 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3121 atcacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3181 atgccaacaa aacagtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3241 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3301 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3361 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3421 tcaagcaaca atttacgcgc gatttgcagc ccggcttcta catcaaaaga gccatccata
     3481 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3541 cgcattacca gatagaggct atcgctgacc tctgcggcaa gagctttaaa tcgacgagca
     3601 tattccagag cagtttccgg atcatgaatg gaacaaggac cacatactac cagcagacga
     3661 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     3721 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     3781 tcggtaatat gtacgttatt cagcgcgtct ttatgcatga tggcgatcct gtttatgctc
     3841 gtttgcgata gttgctcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     3901 caatccatat ttcgtacatt tttatttaca caggcaattt agtcgcgctt tcaacccttg
     3961 cttctgtata gataaattta catccccttt aaaaacaatc cgctatgctt tgaaaaagga
     4021 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4081 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4141 ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4201 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4261 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4321 aaatgccagc caactgcatg aagtatggca aaaaagggct gatgaaaatg ccagcacaat
     4381 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4441 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4501 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4561 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     4621 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     4681 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     4741 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     4801 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     4861 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     4921 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     4981 aacctggatg cctcatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5041 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5101 attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct ggttgctgct
     5161 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5221 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5281 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5341 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5401 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5461 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caatcagcca ttttatctgg
     5521 ttttcgctcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     5581 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     5641 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     5701 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     5761 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     5821 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     5881 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     5941 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6001 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6061 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6121 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6181 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6241 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6301 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6361 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6421 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6481 ctcccggaaa gccagcaaca gatccaaacc atggcagcca aattggcctc gacagggcta
     6541 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     6601 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     6661 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     6721 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttgcaacccc ttaaaacacg
     6781 agcaaacaga caaaaaaagg gccagccaat tggccagccc ttcttaacag gatgtcgctt
     6841 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     6901 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     6961 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7021 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7081 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7141 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7201 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7261 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7321 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7381 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7441 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7501 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7561 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     7621 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     7681 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     7741 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     7801 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     7861 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     7921 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     7981 aaacaggcta attatgccaa tccacatagc gccgcctttt accgtttatc cggtggttta
     8041 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8101 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8161 cttaatgacg agaacgtcat tagatccggt ttccatcata tcaacgactt taccgagatc
     8221 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8281 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8341 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8401 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8461 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8521 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     8581 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     8641 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     8701 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     8761 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     8821 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     8881 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     8941 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9001 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9061 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9121 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9181 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9241 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9301 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9361 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9421 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9481 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9541 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
     9601 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
     9661 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
     9721 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
     9781 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
     9841 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
     9901 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
     9961 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10021 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10081 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10141 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10201 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10261 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10321 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10381 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10441 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10501 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10561 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    10621 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    10681 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    10741 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    10801 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    10861 tcacccatct ggaagcaacg cctgggatgc tgatgcacat tggcttatcg ctcttttcct
    10921 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    10981 tgaagaacaa gaagctggca tttaaccaag aaatgccgcc attgatgagt atcgagcgta
    11041 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11101 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11161 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11221 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11281 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11341 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11401 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11461 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11521 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    11581 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    11641 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    11701 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    11761 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    11821 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    11881 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    11941 tgatgacatc atggtaccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12001 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12061 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12121 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12181 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12241 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12301 gattgttggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12361 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12421 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12481 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc aagtacgata tcgatattct
    12541 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    12601 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    12661 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    12721 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    12781 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    12841 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    12901 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    12961 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13021 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13081 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13141 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13201 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13261 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13321 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13381 ggaaaaatga ataatcattt caagtgtatt ggcattgtgg gacacccacg gcaccctact
    13441 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13501 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13561 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    13621 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    13681 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    13741 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    13801 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    13861 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    13921 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    13981 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14041 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14101 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14161 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14221 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14281 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14341 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14401 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14461 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14521 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    14581 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    14641 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    14701 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    14761 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    14821 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    14881 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    14941 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15001 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15061 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15121 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15181 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15241 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15301 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15361 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15421 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15481 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15541 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    15601 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    15661 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    15721 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    15781 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    15841 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    15901 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    15961 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16021 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16081 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16141 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16201 tgatgttaac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16261 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16321 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16381 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16441 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16501 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16561 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    16621 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    16681 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    16741 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    16801 gtattgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    16861 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    16921 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    16981 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17041 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17101 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17161 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17221 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17281 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17341 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17401 gattgatatg gggtattttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17461 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17521 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    17581 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    17641 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    17701 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    17761 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    17821 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    17881 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    17941 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18001 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18061 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18121 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18181 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18241 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18301 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18361 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18421 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18481 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18541 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgctccgaa
    18601 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    18661 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    18721 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    18781 ctacgttctc tatgatggcg atggccttga gctactcatt aaatccagcg ggagtaaaat
    18841 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    18901 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    18961 agctaaacag attgatccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19021 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19081 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19141 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19201 tcaggccaga ggagcactgg aaacggttcg tcgcctgtgt caacgtatta atgaggtcat
    19261 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19321 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19381 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19441 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19501 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19561 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    19621 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    19681 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    19741 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    19801 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    19861 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    19921 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    19981 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc gggtgtcgcg
    20041 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20101 cggtaatgct gccaacttac t
//
LOCUS       DAJTRM010000076        20007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRM010000076 DAJTRM010000000
VERSION     DAJTRM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.076"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            281..340
                     /gene="ibsE"
                     /locus_tag="OO322_002865"
     CDS             281..340
                     /gene="ibsE"
                     /locus_tag="OO322_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5907674.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(460..1893)
                     /gene="hldE"
                     /locus_tag="OO322_002866"
     CDS             complement(460..1893)
                     /gene="hldE"
                     /locus_tag="OO322_002866"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5907675.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1941..4781)
                     /gene="glnE"
                     /locus_tag="OO322_002867"
     CDS             complement(1941..4781)
                     /gene="glnE"
                     /locus_tag="OO322_002867"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5907676.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4804..6105)
                     /gene="ygiF"
                     /locus_tag="OO322_002868"
     CDS             complement(4804..6105)
                     /gene="ygiF"
                     /locus_tag="OO322_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5907677.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6347..6967
                     /locus_tag="OO322_002869"
     CDS             6347..6967
                     /locus_tag="OO322_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5907678.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7031..8269
                     /gene="cca"
                     /locus_tag="OO322_002870"
     CDS             7031..8269
                     /gene="cca"
                     /locus_tag="OO322_002870"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5907679.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8432..9253)
                     /gene="bacA"
                     /locus_tag="OO322_002871"
     CDS             complement(8432..9253)
                     /gene="bacA"
                     /locus_tag="OO322_002871"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5907680.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9344..9712)
                     /gene="folB"
                     /locus_tag="OO322_002872"
     CDS             complement(9344..9712)
                     /gene="folB"
                     /locus_tag="OO322_002872"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5907681.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9817..10434
                     /gene="plsY"
                     /locus_tag="OO322_002873"
     CDS             9817..10434
                     /gene="plsY"
                     /locus_tag="OO322_002873"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5907682.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10447..11378)
                     /gene="ttdR"
                     /locus_tag="OO322_002874"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10447..11378)
                     /gene="ttdR"
                     /locus_tag="OO322_002874"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11585..12428
                     /gene="ttdA"
                     /locus_tag="OO322_002875"
                     /pseudo
     CDS             11585..12428
                     /gene="ttdA"
                     /locus_tag="OO322_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12425..13030
                     /gene="ttdB"
                     /locus_tag="OO322_002876"
     CDS             12425..13030
                     /gene="ttdB"
                     /locus_tag="OO322_002876"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5907683.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            13078..14541
                     /gene="ttdT"
                     /locus_tag="OO322_002877"
     CDS             13078..14541
                     /gene="ttdT"
                     /locus_tag="OO322_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5907684.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14584..15597)
                     /gene="tsaD"
                     /locus_tag="OO322_002878"
     CDS             complement(14584..15597)
                     /gene="tsaD"
                     /locus_tag="OO322_002878"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5907685.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15835..16050
                     /gene="rpsU"
                     /locus_tag="OO322_002879"
     CDS             15835..16050
                     /gene="rpsU"
                     /locus_tag="OO322_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5907686.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16161..17906
                     /gene="dnaG"
                     /locus_tag="OO322_002880"
     CDS             16161..17906
                     /gene="dnaG"
                     /locus_tag="OO322_002880"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5907687.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18101..19942
                     /gene="rpoD"
                     /locus_tag="OO322_002881"
     CDS             18101..19942
                     /gene="rpoD"
                     /locus_tag="OO322_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5907688.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4765 a         5571 c         5121 g         4550 t
ORIGIN      
        1 ttgtgccttt actttaggtt gaaaaaaaat cagcgtcaag aaggtttcat taacacatat
       61 ccctggccca aaaagccggg gattttttat atctgcgttc cgctaaaagg tgcaaatgct
      121 caggacgttg cagcgttttg cgtgaccgct cggggaaggc aaaactgctt cagggaaagc
      181 gttgcgcggg gtccggcgct catcaacaat cggggagtca gcaaggggct gaaacgggaa
      241 agcccctccc gaggaagggg ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat
      301 actggttgtg ttgttactgt taagtttccc gacttactaa caactgatca gaggggggag
      361 aaatcctccc ttacccttgt tcctttactt taggttgaaa aaacaacagc gtcaataggc
      421 ctgcctgcta cgaagcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg
      481 gatcttcttg atgatgttag tggtcgagca accgtcttcg aagttgagca ccagcacttc
      541 gccgccgttg gcccagactt ctttactccc ggcgatctct tctggtttat agtcgccgcc
      601 cttcaccagt aaatctggca ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa
      661 cgacaccacc cagtcgaccg cttccagtgc gcccagcaca atcatacgct gttcgagcgg
      721 gttaacgggg cgagaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac
      781 aatcaagcgg tcacccagtt tgcgggcatt tgccagatag aagacgtgtc cggcgtgcag
      841 gatgtcaaag acgccattgg tcatcaccac tttttcaccg cgtttacgcg ctgccgctac
      901 ggccagcttc agttcctctt cggtcatcac gccaaagccg gtatctgcgc gcccacgcac
      961 tgcattttcc agctcgatcg gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc
     1021 agccgccgca ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc
     1081 cgccaggacg ccaatcaccg tgtcgcccgc accggtaacg tcatacactt cctgcgcctg
     1141 ggttggcata tgcagcggcg ctttacccgg ttgcagcagc gacataccct gttcggaacg
     1201 ggtcactaac agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat
     1261 ctcttcttcg gtcttacatt taccgacaac agcttcaaat tccgagagat tcggtgtcag
     1321 cagcgtagcg ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcaccggaac
     1381 gcccgcttta cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta
     1441 gtcagaaagc accagcgcgc caatcgaact cagcgcctga ttaatccgct cgtgcagcgg
     1501 ctgcggatca acaccttcga aaccttcttc aaaatccaga cggatcagct gttggttgcg
     1561 ggaaagtacc cgtaatttgg tgatggtcgg atgcgtcggt acagaaacga agtcgcattt
     1621 gacgttgacg tcggccagag atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa
     1681 cccgaccagg cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc
     1741 gcccggacgt tcttcgatgg tattcacttt aaccacgggc accggcgctt ccggcgagat
     1801 acgactggtg gggccgtacc agtaacgatc cagcatcaca tcaccaacca ccatcactcc
     1861 tgcacgttca aactctggca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt
     1921 gcgcgcgata ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc
     1981 agatcacgct ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc ctgtaatgcc
     2041 agatgatgaa gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc
     2101 tcttccataa tgtcgttttg cgccagcagc tccagaatac gcacgttgtc tgaccagcgc
     2161 gttaacttcg gtttttcatg agcgtagcgc aacaccagat attgggtaat aaattcgata
     2221 tcggtaattc cgccttcatc ggctttgata tcaaagcgat tacgatgttt attgccgaga
     2281 tgagcgcgca ttttctcgcg catttcccgc acttccgttt gcagagtttt accttctcgc
     2341 ggcagtgtca taatctcacg acgcaccgcg tcaaagtgcg cagtgagctg cggatcgccg
     2401 tacactacac gcgcacgcac cagcgcctga tgttcccacg tccaggcctc gtttttctga
     2461 taatcggcaa atgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcagt
     2521 cgagcatcca cttcatacaa aatgccggaa gaggtacgcg tactgaacag atgcataatg
     2581 cgttgcgcca gacgcagata aaactgccgc ccgtcgattt cccgctcgcc gtcagtcatc
     2641 gcatccattg ggcaatcatg gaggaagata aggtcaagat cggaactgta gcctaactcc
     2701 cagccgccca gcttgccgta gccgaccacc gcaaaaccgc gcccttcacg ttcgttgagg
     2761 tgatttggct taccgtagcg ggccaccatt tgaccccacg cctgctgaac gacggcatct
     2821 atcatagctt ctgccagcca ggttaagtga tcgctcactt tcattaccgg tagcgtaccg
     2881 gcgatatccg ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca
     2941 agctgttgtt cttcgtcatc ttccggcacg cgcagcaaat actggcgcag ttcatcacgg
     3001 taggcatcgg tcgccgtcgg ctgataaagg gtatttggat cgagcaattc atccagcaat
     3061 aatggataac gcgctagctg gctggcaatc atcggcgatg cggcacacag agaaatcaaa
     3121 tgtttaagcg ccgcagggaa ttcactgagc aattctaaat aggttgtgcg ggtaacaatc
     3181 cccaccagca aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca
     3241 tcacttagca gatgtggcat cagatggtcg agcacctgac gtcctcgcgg cccgatagta
     3301 cgcttatcca gctctttgcg gaaatcagca atcatcgtta ggacctgttt gcgatcatcc
     3361 tcgctaagat gcgccagcac tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc
     3421 tcacgccact gttccgacag cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat
     3481 tcattaaaca cccggcgcac attggtcata tgtgcggtca gcgccccggt cagttgcggc
     3541 cagtcagcaa agtccatcgc ccacgccagc cgcgcacggt taagctcatc agaaggaagc
     3601 gtttgggttt gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga
     3661 tatgccactc gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggcaatggcg
     3721 ctgagcgttg gcagtaaaga gcgcgactgc agcaacggtt cgcgcccacc gcgaatgagc
     3781 tggaacacct gaacgataaa ttcaatttcg cgaatgccgc ctgcgccgag tttgatattg
     3841 tcggtcaaac cacgtcgacg cacttcacgg gcaatcatcc ctttcatgtt gcgcagcgac
     3901 tgaatcacgc tgaaatcgat gtaacgacgg aaaacaaacg ggcgcagcat cgcacgcaac
     3961 tcgttagcat agacgccttc gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc
     4021 tcccagtcgc gtccctgctc ctggtaataa tcctccaacg cggcaaagct cagcaccagc
     4081 gggccacttt cgccaaacgg acgcagtcgc atatccacgc gatagacgaa gccatcctgc
     4141 gttggttgat ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta
     4201 tccagttccc ggcgtccgcc ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg
     4261 atatcagagg agaaattcag ttccccgccg cccagcttac ccatgcctaa gatcagcagc
     4321 ggttgcgctt cgccctgcgc attgcacggc gttccccact cgcggcagca ggcgtcatac
     4381 agccagtcac gcgccgcaac aatcagcgtc tccgccagat aactgagctg ctgcaatatg
     4441 ctctcttcag taaccagtgc cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc
     4501 cggaatagcc gcagctcgcg cattaacccg gcttcgtcgc tcacattact gagcgcctcc
     4561 tgcaaccaca cgctgtaatg ttgccattcg tcagcctgtg gcggttggct ttccagttcc
     4621 gtcagccact ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa agtaagtact
     4681 gacttcgcct gtgcactaag tgattcctcg gctaaaagct ctggcagccg ctcaacaacg
     4741 gtctgccagt actgctgtaa cggtgaagag agcggcttca taagcgattt tatccttgcc
     4801 tgattaacgt tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa
     4861 atgttcaatt tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc
     4921 cagccaggct tgcgcgacga caggatcata gtaacccgcc agcaacagta ttgagtcaat
     4981 atcacgcatc aggcgtggca actggtcacg gtagcgatcg cctaacggct ggcagaaaac
     5041 gcttttcagt tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggagtc
     5101 gctaattttg ccctgcgctt tggcatctaa aaacggctgc catgctttgc ttaccagcca
     5161 ttcggtcaac gccagcttcg ccattgccgt ttcggtagag tagaccgccg tcacggcaga
     5221 aaccgcagaa gcgatggtcg cttcgcattg agtcagcaaa tcacgtaagt gagtactcgc
     5281 tttacgcggc acaatgccac cgaacagcat cagtgtatga cggactaggc taatggctgc
     5341 cagcacctgc tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca
     5401 ttgcgctaac accagctcga gcgccgcttc cagcccctgt tccacatcgg cttttgccgc
     5461 aacatgcaaa atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata
     5521 gccacgcgcc gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt
     5581 cgccagtttc agcaccgcgc gcgtgtcgcc gctaagcagc tccagttcca gctcgcagat
     5641 aggctcagca aattcacccg ctttcacttc cccctggtcg agagcgattt caattcggct
     5701 gccatcgatc tctactagcc atttttcgcg ataaaaatcg gtgctaaaca gcgactgcac
     5761 gcgggaagcg agatcggcag gcaattcgcc gttcggccag atttccgtcg gtaactgcgc
     5821 caggtcgagc gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc
     5881 tgtcactctt cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg
     5941 taagcccata tcgtgcctac gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa
     6001 ctgcacgggg tcatggtgct cgccgcccag cgtattgaga tggtcacgca acgcctcaac
     6061 ggcactgtga ttaacaataa actttaattc gatttcctga gccatggcct ttacttatcg
     6121 gttatgtcac ataagtgacg atgaacggcg aacttaatgc gatctttttt gtcagtagat
     6181 agtattttgc gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca
     6241 gtggcaagac ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt agcgacactg
     6301 atgccactac tatcgttcca ctttccatga caataacgac agcctgatgc caaaattacg
     6361 cctgatcgga ttaactttac tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac
     6421 gcgctatgtt tctgacgaac tgaatacctg ggtccgtagc ggtccgggag atcattatcg
     6481 cctcgtgggc acggttaacg ccggcgagga agtgacctta ttacaaactg acgccaacac
     6541 caattatgcc caggtgaaag acagctctgg ccgtaccgcc tggatcccgt tgaaacaact
     6601 tagcactgag ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct
     6661 gaccgataaa ctcaccaata tcgataacac ctggaatcag cgcaccgcag aaatgcagca
     6721 aaaagtggcg cagagcgaca gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa
     6781 aaacgagctg attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa
     6841 acagcgcacc atcatcatgc aatggtttat gtatggcggt ggcgtgctgg ggcttggctt
     6901 gctgctcggt ctggtactgc cgcacctgat cccaagccgc aaacgcaaag atcgctggat
     6961 gaactaaatc gccttcttcg ttgcactgtc ttattattag gaattgtgta tttgacgaag
     7021 ggatgatggc gtgaagattt atctggtcgg tggtgctgtt cgggatgcat tgttaggact
     7081 atcggtcaaa gacagagatt gggtggtggt cggcagtacg ccacaggaga tgctcgacgc
     7141 gggctaccag caggtaggcc gcgattttcc tgtgtttctg catccgcaaa cgcatgaaga
     7201 gtatgcgctg gcacgtaccg aacggaaatc cggttccggt tacaccggtt ttacttgcta
     7261 taccgcaccg gatgtcacgc tggaagatga tcttaagcgt cgcgatctga ccattaatgc
     7321 gctggcccag gacgataacg gcgagattat cgacccgtac aacggtctgg gcgatctgca
     7381 aaatcgtctg ttgcgccatg tttcccccgc ttttggcgaa gatccgttac gcgtactgcg
     7441 cgtggcgcgt tttgctgcgc gttatgccca cctcggtttt cgtattgccg atgaaactct
     7501 gacgctgatg cgcgagatga cccatgcggg tgaactggaa cacctgacgc ctgaacgggt
     7561 atggaaagag acggaaagcg ccctcaccac ccgcaatccc caggtgttct tccaggtact
     7621 gcgcgattgc ggcgcacttc gcgttttatt cccggaaatt gacgcactgt ttggcgttcc
     7681 ggcacctgcc aggtggcatc cggaaatcga cacgggtatt cataccttaa tgacgctctc
     7741 aatggcggcg atgctgagtc cgcaggttga tgtccgtttc gcaactttat gccacgatct
     7801 cggtaaagga ctgacgccgc cagaactttg gccgcgtcat catgggcatg gcccggcggg
     7861 tgttaagtta gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt
     7921 agccagactg gtggctgagt ttcacgatct catccgcacc ttcccaatgc tgaacccaaa
     7981 aaccatcgtc aaattatttg attccatcga tgcctggcgt aaaccgcagc gtatcgagca
     8041 actggcgctg accagcgagg ctgacgtgcg cggcagaacc ggttttgaat cagcggacta
     8101 cccgcaaggc cgttggttgc gcgaaggctg ggaagtggcg cagtcagtgc cgacaaaagc
     8161 ggtcgttgaa gcgggattta aaggcgtgga gattcgcgag gagctgaccc gacggcggat
     8221 tgcggcggta gccagctgga aggaacaacg ttgcccaaag cctgactgag tataaaacta
     8281 aacgttagca cgaaccgtcc cctcgcccct tcggggagag ggttagggtg aggggaacag
     8341 accagcaccg gtgcgaacaa tcacccccac cccggccctc tccctacaga gcgagggaga
     8401 aaacccggcc tgctcccgaa ccttttctca atcaaaagaa cacgacatac accgcagccg
     8461 ccacaataaa gcgataaatg gcgaacggga taaacgaaat gcgcttaatc aattgcagga
     8521 aggttttaat cgctatcagc gccaccacaa aagcggtgat aaacccaacg gcaaacatcg
     8581 ggatatcgcc ggttgtcagg aagccccagc ttttgtagag atcgagcgcc gttgcgccca
     8641 tcatcatcgg taccgccagc aggaacgaga actcggaagc agcgtaacgg ctcacgccca
     8701 tcagcatccc acctgaaatg gtcgccccgg aacgggagaa acccggccac agcgccagac
     8761 actggaaaca gccaatcata aacgcctgac gataggtcat atcatcaaga cccggcgcac
     8821 gcggctcttt cggcttcagg cattcggcgg caatcagcaa caaaccgcca acgaccagcg
     8881 catacatcac atttatcggg ttaaacaatg acttaatcgt gtcgtggaat aatagcccca
     8941 gtaccaccgc cggaatcatc cccagcaaaa tgtggatcag cgttaaacga cctttgcttt
     9001 caccttcgtg ctgcaacggg cggccaaagt ggatgccaat caggccaaac agacgccgcc
     9061 agaacatcac tactaccgcc agaattgatc ctaactggat cacaacttca aaggttttcg
     9121 ccgtgtcgcc ctcaaacccc aacaagtgac cgacaataat catatggccc gtgctggata
     9181 ccggcagaaa ttctgtcaat ccttcgacca cacccaatat tgccgctatc agcagcgagt
     9241 gcatatcgct catcaataaa cccctaaatt attaaaatgt accgcttgtc cgaactactg
     9301 cagtatgacc aggttataac cgtttggttt aacagctgta aaattaatta ttttctttca
     9361 gattattgcc acgctcaatg attacgccaa cattcgccgc ccgcgccact gcgcctggct
     9421 tgctgagttt gatacgcacc cacggcgagt tgaagcgtgc taacagcagc tccgccacct
     9481 cttcagccac gcgttccacc agcgcaaaac gcgccccctc gacgtggctg accaccgttt
     9541 ctgcaatgtc agcgtaactg aggcaatccg ccacatcatc acttttcgcc gctttacggt
     9601 tatcccatgc catttcgata tcgaacacta acttctgttc gatggtctgt tcccagtcgt
     9661 aaacaccaat agtggtgatt accgaaagtt gctctataaa tacaatatcc atcacgtcct
     9721 gcctgctttt tggctaaccg gataccactt ccggcgaaat gtgcgtatta tccacagatt
     9781 catcgttgaa cacgaatttt caaaacggaa cagcttatga gtgcaatcgc gcctggaatg
     9841 atcctcatcg cgtacctctg cggctccatt tccagtgcca ttctggtttg ccgcttgtgt
     9901 gggctgcccg atccgcgaac cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt
     9961 atcggtggca agggagcagc cgtagcagta ctgattttcg acgttctgaa aggaatgttg
    10021 cctgtctggg gcgcgtatga attaggtgtc agccccttct ggctaggctt aattgccatc
    10081 gccgcctgtc ttggacacat ctggcccgtt ttcttcggtt ttaaaggagg aaaaggcgtt
    10141 gctaccgctt ttggagcaat cgcaccaatt agctgggatc tcaccggagt aatggcggga
    10201 acctggttac tgaccgtgct attgagcgga tactcgtcgc tgggagcgat tgtcagtgca
    10261 ctgattgctc cgttttatgt ctggtggttt aagccacaat tcaccttccc ggtttcgatg
    10321 ctctcttgcc tgatcctgct gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag
    10381 gagacaaaaa tctggacgaa attcaaaaga aagcgcgaaa aggatcccga gtgatttctg
    10441 gtggatctac atgacctgat agccttcatc gggcttgccc agccgttgct ggcaccatgc
    10501 cgccagaaat tccgcgcaga cacgaatttc atgctgcgat agagcagctc ccggtaaaca
    10561 gcccagatat tggcgctttg tgcatactcc ggcaatactt gcaccagttt gccactctcc
    10621 agaaacggca acacatccca ctcggaacgc agcataatcc ctttgccctc cagcgcccat
    10681 tgcaacacta tctcgccgct attggaggaa agatgcccgc taaccttcac cgattttttc
    10741 tcctgaccgt tccccaactc ccatattccg tgggtcatat cgcgctcttt ggtcaccaga
    10801 cagtcatgac gacttaattc ctgtaatgat tgtggctgcg gatatttttg cagatactca
    10861 ggtgctgcgc ataatattct tttatttttc gttaacagat gcgcaatata ataatcagga
    10921 atttcgtcat taatacgaat atccagatca atattatcct gcactaaatc aatttgccga
    10981 tcgaacaatt caaaatgcac ctgtaactca ggataattgc gcataagttc ggtaatggct
    11041 ggcgcaatat ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat cccctccggg
    11101 cgcgttttga tttgcgtgac gtcatcgacc agtcgctgat attgggtgag gatttccagc
    11161 gcatgttcgt aacaacgttg gccactttca gtcagcgcca cgccgcgagc ggagcggttc
    11221 agcagcgttg ttgccagcgt attttcaagg atttggatcc gcttggtgac aaatgcaggc
    11281 gtttgcccaa gcgtcgccgc tgcagcacta aaactcccgc tgtggacgat ttctaccagt
    11341 acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga
    11401 taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt
    11461 attttcgcgt tgattcacga actgttaatt acatattaac ggtggcaaaa aatataaaac
    11521 cacattttta gtgttagttt gtggcggtga atttttccag acaaatacaa aaactggagt
    11581 tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc
    11641 taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca
    11701 gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa
    11761 catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt
    11821 cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca
    11881 agccgtggaa gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga
    11941 cgaagtaaac accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat
    12001 ccccgacaat gacgatgcgg aaatcgaagt ttacatggca ggcggcggct gcacgctacc
    12061 tggccgctcg aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt
    12121 cgaaaatatc tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat
    12181 cgccacctcg gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg
    12241 cagccgccat cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa
    12301 ccgtctgggg attggtccac aagggctgac cggcaacagt tcagtgatgg gcgcatcgtc
    12361 gcggcacgct gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg
    12421 cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag atattcgcgt
    12481 cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgcgacgttt gtcaccgccg
    12541 tttgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc
    12601 tggccccatc gtgcgcaaaa acggcgacaa atgggagatg gtctccgtcg gcccgacaac
    12661 cagtatgcgt atggaaagtt ttgaacgtga atttattgag cagaccggcg taaaactggt
    12721 ggttggcaaa ggtggtatgg ggccgctgac cgaagaaggc tgccagaaat tcaaggcgtt
    12781 acatgtgatt ttcccagcag gctgcgcggt gctggcggca acccaggtag aagagattga
    12841 agaagtgcac tggacagaac tcgggatgcc ggagtcactg tgggtctgcc gggtcaaaga
    12901 gttcggcccg ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa
    12961 aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta
    13021 catcaaataa ccctcccgga gaggctcacc cctctccttt ttcgcaggca taacacgatg
    13081 aaaccttcca ctgaatggtg gcgatatctt gcgccgctgg cggtcatcgc cattattgct
    13141 ctacttccgg ttcccgcagg gctggagaac catacctggc tctactttgc cgtttttact
    13201 ggcgtgatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt
    13261 atctccatca tcgccatact ctctccctgg ctgctgttca gccccgaaca gctcgcccag
    13321 ccaggcttta agttcaccgc caaatccctc tcctgggcag tttctggttt ttctaattcg
    13381 gttatctggc tcattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt
    13441 ggacgccgca ttgcgctgat tctggtgaaa aagatggggc atcgcacgct gtttctcggc
    13501 tatgcggtga tgttctccga gctgatcctg gcacctgtaa caccgtccaa ctcggcgcgt
    13561 ggtgcgggta ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca
    13621 aacgacagca gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac
    13681 tgcgtgacca gcgccatttt cctgacggcg atggcaccta acttgctgtt aattggactg
    13741 atgaaaagcg catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg
    13801 ctcagcattt tactggttct gctggttccc tggctggctt acgtgctgta cccgccggta
    13861 ctgaagtctg gtgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg
    13921 ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt gctgtggatt
    13981 ttcggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc gctgatgctg
    14041 cttctgcgca ttatcagttg ggacgacatt gtcagtaata aagcggcatg gaacgttttc
    14101 ttctggctgg cctcgcttat caccctcgcg accggactca acaataccgg ttttattagc
    14161 tggtttggca aactgttagc aggcagctta agtggttatt cgccgacgat ggtgatggtg
    14221 acgttgattg tggtgtttta tctactgcgc tactttttcg ccagcgccac ggcgtatacc
    14281 tgcgccctcg caccgatgat gatcgccgcc gcgctggcga tgccggaaat cccgctgccg
    14341 gtgttctgcc tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc
    14401 accggaccca gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga
    14461 ctgggggcga tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg
    14521 atgcccgtgg tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata
    14581 gttttacgca gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc
    14641 cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc
    14701 agtacaaaat tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc
    14761 cagcttcgcc cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa
    14821 gcccgtctga tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc
    14881 aaaggcgcgg gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt
    14941 cgccgcaaag gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg
    15001 acgcgggaaa acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc
    15061 gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc
    15121 atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt
    15181 atgaccgccg gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat
    15241 cggcgctaac agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa
    15301 cgccagagaa cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc
    15361 ggtataggcc acagcatcaa tgtcttttgc cgttaaacca gactccttta gcgccgcctg
    15421 gatcaacggt acggttttac gcacatgatc gcgggaggcc agttcaggca cgacgccgcc
    15481 gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc
    15541 gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac
    15601 ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt
    15661 tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa
    15721 aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa
    15781 ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg
    15841 gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc
    15901 tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact
    15961 accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc
    16021 gaaaacgcac gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac
    16081 cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga
    16141 attgctaaaa atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc
    16201 tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca
    16261 agaatttcca cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg
    16321 gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc
    16381 tgatgaacta cgacaagctc gagttcgtcg aaacggtcga agagctggca gcaatgcaca
    16441 atcttgaagt gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc
    16501 aaacgcttta tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac
    16561 ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg
    16621 ctcgctttgc gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg
    16681 gcaatccaga aaatcgccag tcattgattg atgcggggat gttggtcact aacgatcagg
    16741 gacgcagtta cgatcgtttc cgcgaacggg tgatgttccc cattcgcgat aaacgcggtc
    16801 gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc
    16861 cggaaacaga cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg
    16921 ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg
    16981 cgcaatacgg cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca
    17041 tacaactgtt gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag
    17101 gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc
    17161 agctacgctt tatgtttttg ccggatggcg aagaccctga cacgctggta cgaaaagaag
    17221 gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc actctccgca tttctgttta
    17281 acagtctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc
    17341 tggcactgcc attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg
    17401 aattaggcaa caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag
    17461 cggcagagag cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac
    17521 ttatagggtt gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc
    17581 tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac acttgtctct
    17641 cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg
    17701 ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa
    17761 ccttcaccga ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt
    17821 taatcgctcg tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat
    17881 taaaccagga gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa
    17941 gcccccgaca gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc
    18001 gttggcggta aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg
    18061 cccaacacca acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc
    18121 acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt
    18181 caatgaccat ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat
    18241 gatcaacgac atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct
    18301 ggctgaaaac accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt
    18361 ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac
    18421 cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat
    18481 caaccaggtt caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca
    18541 gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga
    18601 cccgaacgca gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca
    18661 ggaagatctg gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga
    18721 tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca
    18781 gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga
    18841 agagatcctg aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga
    18901 ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat
    18961 catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac
    19021 cggcaacgaa accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc
    19081 ggaaaaactg cacgatgtct ctgaagaagt gcaccgcgcc ctgcaaaaac tgcagcagat
    19141 tgaagaagaa accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat
    19201 cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt
    19261 tatttctatc gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga
    19321 aggtaacatc ggcctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt
    19381 ctccacctac gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc
    19441 gcgcaccatc cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc
    19501 tcgccagatg ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat
    19561 gctgatgccg gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat
    19621 ggaaacgccg atcggtgatg atgaagattc gcatctgggg gatttcatcg aggataccac
    19681 cctcgagctg ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cgcacgacgt
    19741 gctggctggc ctgaccgcgc gtgaagcaaa agttctgcgt atgcgtttcg gtatcgatat
    19801 gaacaccgac cacacgctgg aagaagtggg taaacagttc gacgttaccc gcgaacgtat
    19861 ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgtt
    19921 gcgtagcttc ctggacgatt aatcggtagg ccggatcagg cgttacgccg cacccggcac
    19981 tagtctctct gcacaaacgc caccttt
//
LOCUS       DAJTRM010000077        19690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRM010000077 DAJTRM010000000
VERSION     DAJTRM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.077"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            3..206
                     /locus_tag="OO322_002882"
     CDS             3..206
                     /locus_tag="OO322_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5907689.1"
                     /translation="MDLGWSPGTELTVEKKGDSVNLRATEHKPRGRLTVAQLLSQIDE
                     SEITELNQSTEGWAEGKKGNEAW"
     gene            200..565
                     /locus_tag="OO322_002883"
     CDS             200..565
                     /locus_tag="OO322_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5907690.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(566..1354)
                     /gene="bioH"
                     /locus_tag="OO322_002884"
     CDS             complement(566..1354)
                     /gene="bioH"
                     /locus_tag="OO322_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5907691.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1392..2075
                     /gene="gntX"
                     /locus_tag="OO322_002885"
     CDS             1392..2075
                     /gene="gntX"
                     /locus_tag="OO322_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5907692.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2134..2709
                     /gene="nfuA"
                     /locus_tag="OO322_002886"
     CDS             2134..2709
                     /gene="nfuA"
                     /locus_tag="OO322_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5907693.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3070..4386
                     /gene="gntT"
                     /locus_tag="OO322_002887"
     CDS             3070..4386
                     /gene="gntT"
                     /locus_tag="OO322_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5907694.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4431..6515)
                     /gene="malQ"
                     /locus_tag="OO322_002888"
     CDS             complement(4431..6515)
                     /gene="malQ"
                     /locus_tag="OO322_002888"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5907695.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6525..8918)
                     /gene="malP"
                     /locus_tag="OO322_002889"
     CDS             complement(6525..8918)
                     /gene="malP"
                     /locus_tag="OO322_002889"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5907696.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9530..12235
                     /gene="malT"
                     /locus_tag="OO322_002890"
                     /pseudo
     CDS             9530..12235
                     /gene="malT"
                     /locus_tag="OO322_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12278..13294)
                     /gene="rtcA"
                     /locus_tag="OO322_002891"
     CDS             complement(12278..13294)
                     /gene="rtcA"
                     /locus_tag="OO322_002891"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5907697.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13298..14524)
                     /gene="rtcB"
                     /locus_tag="OO322_002892"
     CDS             complement(13298..14524)
                     /gene="rtcB"
                     /locus_tag="OO322_002892"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5907698.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14705..16303
                     /gene="rtcR"
                     /locus_tag="OO322_002893"
     CDS             14705..16303
                     /gene="rtcR"
                     /locus_tag="OO322_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5907699.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16285..17043)
                     /locus_tag="OO322_002894"
     CDS             complement(16285..17043)
                     /locus_tag="OO322_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5907700.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17060..17890)
                     /gene="glpG"
                     /locus_tag="OO322_002895"
     CDS             complement(17060..17890)
                     /gene="glpG"
                     /locus_tag="OO322_002895"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5907701.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17935..18261)
                     /gene="glpE"
                     /locus_tag="OO322_002896"
     CDS             complement(17935..18261)
                     /gene="glpE"
                     /locus_tag="OO322_002896"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5907702.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18451..>19690
                     /gene="glpD"
                     /locus_tag="OO322_002897"
     CDS             18451..>19690
                     /gene="glpD"
                     /locus_tag="OO322_002897"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5907703.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESL"
BASE COUNT         4597 a         5450 c         4985 g         4658 t
ORIGIN      
        1 ccatcgatct tggctggtca ccgggcacag agcttaccgt ggagaaaaaa ggcgatagcg
       61 ttaatttgcg cgccacagag cacaaaccgc gtgggcgtct taccgtcgcg cagttgttga
      121 gccaaatcga tgaaagcgaa attacggagc taaatcagtc cacagaagga tgggctgaag
      181 ggaaaaaagg taatgaagca tggtaaaggc acggacgcca catcgtggtg agatctggta
      241 ttttaaccct gatccggttg ccgggcatga acttcagggg ccacattatt gcattgtggt
      301 aacggacaaa aaactcaaca atgttttaaa agttgctatg tgctgcccga tttcaacagg
      361 ggcaaatgca gcacgttcca caggggtgac ggtgaacgtc ctcccccgtg atacgcaaac
      421 cggtaacctg catggcgttg tactttgtca ccagctaaaa gccgtcgatc ttattgcccg
      481 tggcgctaaa tttcataccg ttgccgatga aaaattgatt agtgaagtta tcagtaaact
      541 ggtgaattta atcgacccac aataattagc tctcacttac caccaacacc ctctgcttca
      601 acgccaccag caggtgacaa aactcgaccg gatgcgaaat aaacggcgca tgggccgctt
      661 tggcgaagat atatgattcg ctgtgaggcc aaagtttatc cagcatcggc accactttgc
      721 gcggcaccag accgtcgaga tagccataca atcgcaaaaa cggcatggac acgttttgca
      781 gcggctgacg gagatcgacc gttttcagga tttccagccc gccattaagc acgtcaacct
      841 ccggcatcgg taacgccaga acggttttct tcaacgcccg cgcatcctgg cgcgccgttt
      901 cagtccccat ggtttgtaac gccaggaacc gctccactgt acgctgaaaa tcatcactga
      961 gttgctgctg aaatcccgcc agcacgtccg gttttatccc cggccactcg tcacgagcac
     1021 taaaacaagg tgacgacgcc acggtgacca gcgcctgaac acgctcggga tgggttaacg
     1081 caatctggct tgccaccagc ccgcccagac tccagcctaa ccaaatggct ttatcaggtg
     1141 cctgttgcag cacggcttcg gccatatcag caagtgacag cgcaccaaat ccccggctac
     1201 gcccgaagcc gggcaggtca acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa
     1261 tgcaacgcca cacttcggca ttcagtcccc atccgtgcag cagcacaaga tgaacattcc
     1321 cctgaccttt ggtctgccac cagatgttat tcatccgcta ttgttctctt ttgacttaca
     1381 aggatgaaca tatgctaaca gtaccgggat tatgctggct atgccgaatg ccactggcgt
     1441 taggtcattg ggggatttgt tcggtctgtt cacgcgccac ccgcacagat aaaacgttat
     1501 gcccacaatg tggattaccc gccacacact cccatcttcc ctgcggtcgc tgcctgcaaa
     1561 aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc
     1621 ttatccacca gcttaaattt tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt
     1681 tactattgga agtcttacac gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca
     1741 tcagcgttcc gttatggcag cggcgtcact ggcgtcgggg atttaatcag agcgatttgc
     1801 tgtgtcagcc gttatcacgc tggttgcact gccaatggga tagcgaagcc gtcacacgta
     1861 cacgggccac tgcgacccag cattttctca gtgcccggct gcgcaagcgc aacctgaaaa
     1921 atgcctttcg tcttgaattg cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg
     1981 ttaccaccgg aagtaccgtc gcagagattg cgcagttgct tttacgcaat ggtgcggcga
     2041 ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct cgatgatggg cgtattataa
     2101 ccaactaaaa tagtcaacta ttaggccatt actatgatcc gtatttccga tgctgcacaa
     2161 gcgcactttg ccaaactgct ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg
     2221 attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg
     2281 gaagccaccg acactgccct gaaatttgac ctgctgaccg cgtatgttga tgagttaagc
     2341 gcaccatacc tggaagatgc agagatcgac tttgttaccg accagttggg ttctcagtta
     2401 acgctgaaag ccccgaacgc caaaatgcgt aaagtggcag acgatgcacc gctgatggag
     2461 cgcgtggagt atatgctgca gtcgcagatc aacccacagc ttgctggtca cggtggtcgc
     2521 gtttcgctga tggaaatcac cgaagacggt tacgccattc tgcaatttgg cggcggctgt
     2581 aacggttgtt ccatggtcga tgtgacgctg aaagaaggga tcgagaagca gctgctgaac
     2641 gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc
     2701 tactactaag ttatcccctc ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg
     2761 ggcctacgtt gaacatgtgc cagacgttgg cacagcattg ctttggcaaa tccctccctt
     2821 cgtttccccg cgataatatg accaacctct cataatttaa atttaccccg ctctggtgat
     2881 tctcaaacgc caaatgttac ccgtatcatt cacatgggta ccaaacatac tcctgacatc
     2941 tgactacaat aattagtttt agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg
     3001 attgggataa ttagagtttg tcgtcagaaa attgacgtta cccataacaa atgaaaggcc
     3061 aggtaaatca tgccattagt cattgttgct atcggtgtaa tcttgttgtt gctcctgatg
     3121 atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta
     3181 atgcaaggaa tgccgctgga taaagttatt ggctccatca aagccggtgt cggcgggacg
     3241 ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc tgggcaaaat gctggcagac
     3301 tgcggtggcg cacaacgtat cgccaccacg ctgattgcca aatttggtaa aaagcacatc
     3361 cagtgggcgg tggtgttaac cggttttacc gttggttttg ccctgttcta tgaagtgggc
     3421 ttcgtgctga tgctgccgct ggtgtttacc atcgcggctt ccgcgaatat tccattgctg
     3481 tatgttggtg taccaatggc ggctgcactg tctgtgaccc acggcttcct gccaccgcat
     3541 ccgggcccga ctgcgattgc caccattttc aatgccgata tgggtaaaac cctgctgtac
     3601 ggtactattc tggcaatccc gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg
     3661 aaaggtatcg ataagccaat cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa
     3721 gagatgccga gctttggcgt cagcgtctgg acctctctgg tgccggttgt gctgatggcg
     3781 atgcgtgcga ttgccgaaat gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag
     3841 ttcctcggtg acccggtaat ggcaacgctg attgccgtgc tgattgcgat gttcaccttt
     3901 ggtctgaacc gtggtcgttc aatggatcag attaacgaca cgctggtttc ttccatcaaa
     3961 atcattgcga tgatgctgtt gatcatcggt ggtggcggtg cgttcaagca ggtgctggta
     4021 gacagcggcg tggacaaata cattgcttcc atgatgcacg aaaccaacat ttctccgctg
     4081 ctgatggcct ggtcgattgc tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg
     4141 gcaatcactg cgggtggtat cgcggcaccg ctgattgcaa cgacgggtgt tagcccagag
     4201 ctgatggtta ttgcggtggg ttccggtagt gtgattttct ctcatgtgaa cgatccgggc
     4261 ttctggctgt tcaaagagta ctttaacctg actatcggcg agaccatcaa atcctggtcg
     4321 atgctggaaa ccattatctc ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg
     4381 atttgataac actgactgcc ggatgcatcg catccgacat caaccgcact ctacttcttc
     4441 ttcgctgcag ctctgcgccg tctgtccaaa tccttcagca acttgttcac gccatcatcg
     4501 gcaaacatcg actcaagcgt tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg
     4561 gtgccaggaa tattcaccgg ttcggccata tccagccagt cttccggctg tagtcctaac
     4621 agagcactgt tactgtcggc aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc
     4681 gacatcaacg atgccttatg cccggcacgt tttggcagac aaccatattt atgcagtgca
     4741 tccagcagcc cttgcttcgc cagttcgcga tcctgataca gaccgcgcag taccacttca
     4801 tccggataca gccccagggt tttgcccagc gttagatccc cgctctccca gtaaccacgc
     4861 agcgttggca ggtcatgtgt cgccgcaacc gccatcgact gctccggata cgctttcggt
     4921 gcacggaacg tcttctcgtg gtcgttttcg aaatagagca ctttgtaaga gtacacaccg
     4981 ctgctgcgca gcttaccaac aatctctacc ggtacggtac cgagatcttc accaatcacc
     5041 atacagcgat gacgtttact ttcgagtgcc agaatcgaga gcagatcatc caccggatag
     5101 tgaacatacg cgccctgatc tgccgtctcg ccatacggta tccaccacaa acgcagcatc
     5161 gacatcacat ggtcaattcg taatgcgccg cagttttgca tattggcacg caacagctcg
     5221 ataaacggtt cataggcacg cgcggtgatg atatgcgggt ccattggcgg taatccccag
     5281 ttctgcccca acgggccgag gatatccggc ggcgcgccaa ccgatgcttt caggcaatat
     5341 agttcacggt cacaccaggt ttccgcccca ccttccgcta cgccaaccgc cagatcacga
     5401 tacaagccaa tcggcatttc atagccctgg cttatctccc agcaggcggc aaactggctg
     5461 taagccagcc actgcaacca gagataaaaa tcgacgtcat cacgatgttc ttcgcagaac
     5521 tgacgcactt ctggtgaatc cacgttctta tacatctctg gccatgcagg ccagccccag
     5581 cgcatttcgt cctctttcac ttgctgggca tgtagcgcat caaaggcagc ctgccagaac
     5641 aggctgtcgc cctgctctgc aacaaactgg cgaaacgcgg ccatctgctc atcatcacgt
     5701 tgcgcgaaac ctttccacgc cattcgtaat gctgtcattt ttagggcggt aaccgtggag
     5761 taatcgaccc agtcggcatc gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc
     5821 caccaggcct gagcctcttc gctaagatgg aaatcttcaa cggcgttaac gtcgatataa
     5881 atcacattca gccaacggcg agaagacggg ctgtatgggc tggcgctctc cggatttgcc
     5941 ggatagagcg catgaatcgg gttcaggcca atgaacgacc cgccacgttt tgccacatcc
     6001 accagcatcg ctttgagatc gccaaaatcc ccaatacccc agtttttttc cgatcgcagc
     6061 gtataaagct gaacgcaggc accccacagc ttttgtttat tcagcaaagc ctgcggttcg
     6121 taacaacgtt tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt
     6181 gttagcgtgt gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtt
     6241 acatggcctt tgtactgcgt tccttcttcg gtggtcagct gccagctata ttcgccgctg
     6301 ccctccacca ccatcggcat ttttttgccg ctggtataaa ccatgacatt cgggactggc
     6361 gttaccgcca ctttcgtggc ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt
     6421 tcggcgctaa tcgactgcgg tttaccgtgg gcattgatgt aattggggct aatccccgcc
     6481 gccagcgcgg cattatccag acgtttgctt tccatcgagc ttccttagcg ttttgcctgc
     6541 cagatacgag cctgataatc gcgaatagag cgatccgagc taaacatacc gcagcgggcg
     6601 gtattgagga tcgccgcgcg agtccaggcc tcctggtcgc ggtacagcac atccacctgc
     6661 ttttgtgcct caacataggc tgcgaaatcc gccatcacca gatacggatc gccgccctgt
     6721 ttgccgatac tgtgcagcat ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg
     6781 ctttccagct ctttcaatac tgcgtccagc accttatctt tcttccgcca tttcaccggg
     6841 tcgtagcctt tggccagaat agccttcact tgttccacgg tatgaccaaa aataaagata
     6901 ttttcttcac cgactttctc ggcgatttca acgttcgcac catccagcgt accgacagta
     6961 agcgcaccat tgagcgccag tttcatattg ccggtaccgg aagcttcttt acctgcagtc
     7021 gaaatttgtt cggagatatc cgccgccggg atcagttttt ccgccgccga aacgcaataa
     7081 tccggcagga acaccacctt caacttatcg ccaaccagtg gatcgttgtt gatcacgtca
     7141 gccactttgt tgatcgcaaa gataatattc ttcgccaggt agtagcccgg tgccgctttc
     7201 gcgccgaaga ggaagacgcg cggtacgcga tcagcctgca ggttttcacg aatttctttg
     7261 tacaacgcca gaatatgcag cagattcagg tgctggcgtt tgtactcatg cagacgtttg
     7321 atctgaatat cgaaaatcgc ctgtggattg atctcaatac cggtacgaac tttcacaaac
     7381 tccgccagac agactttatt cgcctgcttg atctcgcgat attgctgacg gaatttcgca
     7441 tcatcagcga atttttccag attgatcagc tgatcgagat cgttagccca ctctttttgc
     7501 agtgatttat ccaacagagc cgccagtgca gggttgcact gtttgatcca gcgacgtggg
     7561 gtaataccgt tggtaacgtt atggaatttg ttcggccata gctggtgata ttccgggaac
     7621 agatctttca ccaccagatc cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca
     7681 accacacaca ggttcgccat atgcacttgt ttatcgtgca ccaccgccag tttggcccac
     7741 actttttcat cgcccggcca ggttttctcc accagcgttt taaagcgagt attaatttcg
     7801 ttaataatct gcatatggcg cggcagtaag cctttcacca gtttcacatc ccagcgttcc
     7861 agcgcttctg gcatcagggt atggttggtg taagcgaaag ttttgctggt gatggcccag
     7921 gcgtcatccc agctcatctg gtgctcatcg gtcagcacgc gcagcagttc tggaatcgcg
     7981 atagttggat gggtatcgtt cagctgaata acttcgtaat ccgccagttc gtgcagttta
     8041 cgccccgcca gatgatggcg acgcaaaata tccgctaccg aacaggcaca ctggaagtat
     8101 tgctgcatca ggcgcagctt tttaccggca gtatggttgt cgtttggata gagaactttg
     8161 gtcagttttt ccgcattgat gccttgctgt tcggcgcgca ggaaatcacc gtcgttaaat
     8221 ttagttagat caaacggatg cgcatgcgtc gcctgccaca gacgcagcgg ctgcgccacg
     8281 ccattacgat agccgacaac ggggagatcc cacgcttgac cggtaatggt aaactccggc
     8341 tcccagcgtc cgtctttcat cactttaccg ccaatcccta cctgcacatc cagtgcttcg
     8401 ttgtggcgga accacgggta gttactgcga tgccagtcat ccggcgcttc aacctgtttg
     8461 ccatcgacaa aagactggcg gaacaaacca tattgatagt tcagaccgta acccgtcgca
     8521 gactgaccga cagttgccat tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg
     8581 ttacccagcg ccgggtcgat ctcttcttcc agcaggtcag tcaggttgat gtcataagcc
     8641 ttcaacgaat cctgtacatc ctgataccag ccgagattca acaggttgtt gcccgtcaga
     8701 cgaccaatca aaaactccat tgagatgtag ttaacatgtc gctgattcgc caccggtttg
     8761 gcgaatggct gagcacgcag catttcggcc agtgcttcac tcactgccag ccaccactgg
     8821 cgaggagtca tttcagccgc agaatttaag ccataacgct gccactgacg tgaaagcact
     8881 tcctgaaatt gcttatcgtt aaaaataggt tgtgacatag gagttccact tttcttagat
     8941 tttcaacaca acgttatcgc tagtttgcca ggctcgatgt tgaccttcct catcctgcgg
     9001 gggattaggc agggaggagt tgcggggatg agcaaggaaa tgtgatctcg accacttaaa
     9061 gctagtgcaa accacaggat tagcagcaaa tcaatgccat acagcgcaga aaatctgtat
     9121 ctaagtgcaa aaaatggccg ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta
     9181 agtaaaggag tgaaacagtt tcacaagtaa aatatccagt gtgctccatc tcattcttaa
     9241 tagatttatt aagatcatct ttttagatgg cactttcatc aggaatgaag aagaaaccct
     9301 tgcttaaatg aatctgatga acataaggga aaccagtatt cacgctggat cagcgtcgtt
     9361 ttaggtgagt tgttaataaa gatttggaat tgtgacacag tgcaaattca gacacataaa
     9421 aaaacgtcat cgcttgcatt agaaaggttt ctggccgacc ttataaccat taattacgaa
     9481 gcgcaaaaaa aataatattt cctcattttc cacagtgaag tgattaacta tgctgattcc
     9541 gtcaaaacta agtcgtccgg ttcgactcga ccataccgtg gttcgtgagc gcctgctggc
     9601 taaactttcc ggcgcgaaca acttccggct ggcgctgatc acgagtcctg cgggctacgg
     9661 aaagaccacc ctcatttccc agtgggcggc aggcaaaaac gatatcggct ggtactcgct
     9721 ggatgaaggt gataaccagc aagagcgttt cgccagctat ctcattgccg ccgtgcagca
     9781 ggcaaccaac ggtcactgtg cgatatgtga gacgatggag caaaaacggc aatatgccag
     9841 cctgacgtca ctcttcgccc agcttttcat tgagctggcg gaatggcata gcccacttta
     9901 tctggtcatc gatgactatc atctgatcac taatccagtg atccacgagt caatgcgctt
     9961 ctttattcgc catcaaccag aaaatctcac cctggtggtg ttgtcacgca accttccgca
    10021 actgggcatt gccaatctgc gtgttcgtga ccaactgctg gaaattggca gtcagcaact
    10081 ggcatttacc catcaggaag cgaagcagtt ttttgattgc cgtctgtcat cgccgattga
    10141 agccgcagaa agcagtcgga tttgcgatga cgtttccggt tgggcgacgg cactatagct
    10201 aatcgccctc tccgcccggc agaataccca ctcagcccat aagtcggcac gccgcctggc
    10261 gggaatcaat gccagccatc tttcggatta tctggtcgat gaggttttgg ataacgtcga
    10321 tctcgcaacg cgccattttc tgttgaaaag cgccattttg cgctcaatga acgatgccct
    10381 catcacccgt gtgaccggcg aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca
    10441 ggggctgttt ttacagcgga tggatgatac cggcgagtgg ttctgctatc acccgctgtt
    10501 tggcaacttc ctgcgccagc gctgccagtg ggaactggcg gcggagctgc cggaaatcca
    10561 ccgtgccgcc gcagaaagct ggatggccca gggatttccc agcgaagcga ttcatcatgc
    10621 gctggcggca ggcgatgcgc tgatgctgcg cgatattctg cttaatcacg cctggagttt
    10681 gttcaaccat agcgaactgt cgctgctgga agagtcgctt aaggccctgc cgtgggacag
    10741 tttgctggaa aatccgcagt tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg
    10801 ctatggcgaa gttaacaccc tgctagcccg tgctgaacat gaaatcaagg acatcagaga
    10861 aggcaccatg catgcagaat ttaacgctct gcgcgcccag gtggcgatta acgatggtaa
    10921 tcaggatgaa gcggaacggc tggcaaaact ggcactggaa gagctgccgc cgggctggtt
    10981 ctatagccgc attgtggcaa cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt
    11041 gacccgctca ctggcgctag tgcagcaaac cgaacagatg gcacgccagc acgacgtctg
    11101 gcactacgcc ttatggagtt taattcagca aagtgaaatt ctgtttgccc aagggttcct
    11161 gcaaaccgcg tgggaaacgc aggaaaaagc gttccagctg atcaacgagc agcatctgga
    11221 acagctgcca atgcatgagt ttctggtgcg catccgagca caactgttat gggcctgggc
    11281 acggctggat gaagccgaag cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca
    11341 gccacagcaa cagcttcagt gcctggcaat gttgattcaa tgctcgctgg cccgtggtga
    11401 tttagataac gcccgtagcc agttgaaccg tctggaaaac ctgctgggga atggcaaata
    11461 tcacagcgac tggatctcta acgccaacaa agtccgggtg atttactggc aaatgaccgg
    11521 cgataaagcc gccgctgcca actggttgcg tcatacggct aaaccggagt ttgcgaacaa
    11581 ccacttcctg caaggtcaat ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt
    11641 tgaacctgcg gaaattgttc tcgaagaact caatgaaaat gcccgcagtc tgcggttgat
    11701 gagcgatctc aaccgtaacc tgttgctgct taatcaactg tactggcagg ccggacgtaa
    11761 aagtgacgcc cagcgcgtgt tgctggacgc attaaaactg gcgaatcgca ccggatttat
    11821 cagtcatttt gtcatcgaag gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact
    11881 taatacgctg ccggaactgg aacagcatcg cgcgcagcgt attctgcgag aaatcaatca
    11941 acatcatcgg cataaattcg cccatttcga tgagaatttc gttgaacgtc tgctaaatca
    12001 tccggaagtg ccagaactaa tccgcaccag cccgttgacc caacgtgaat ggcaggtcct
    12061 ggggctgatc tactccggtt acagcaatga acaaattgcg ggcgaactgg aagtggcggc
    12121 aaccaccatc aagacgcata tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca
    12181 ggctgccgtg cagcacgcgc agaaactgct gaagatgatg ggttatgggg tgtgagttta
    12241 gccggataat gcattggatc cggtttacat ctttacatca ttcaatactc acccgcgtta
    12301 cgccatctgt ttctatcaaa ccaaaccgca ccggcaagaa acgctccacc accgcgatat
    12361 tggtcagcag atggcatgaa ggatgggcga ccgtaaattc tcccgcgccc gccagcgcca
    12421 tcggcagtac cagttggtcg gcgagatatt cccccaccgc cgctgggctt gccaggtagc
    12481 gtttcacctc tttcactaac tgcgcggcta ccacctcggc actgacgcgc ttttcaccga
    12541 cgacgaaaaa gcgttcagtg atattttcac tttcgacttc cagcgagacg gtattacccg
    12601 gcccctggtc gcgcggcagg ttatgaatat tctgttcatg cagggaaaaa atccccgcca
    12661 gtgtagcgat ttcacgctca gcaacatggc gcggcacacc agctaataga acttctccac
    12721 gcatctgcac aatgttcccg cgctcgccaa gttgcaaggt gctaaacgat gccacgggcg
    12781 agacttccgt tgccaccaca ccgcctccgg caggataaaa accgtgacgt aacagcgtgg
    12841 tttgctgatg aattcctatt ttcgccagca gcggctccag cacccggcgg ataaaatccg
    12901 caggcggcgc cgacgggtta tcggtgccgc cgctcacttc aacacgcgaa ggtccatcgg
    12961 caaaccacag cgcgggcagc accgtttgca gcaccagcgt acaacttccg gcgctaccga
    13021 tagcaaagcg gtaatcgccg ccgcgcacgg tgccaggccg gaagaccaga cgctgcgatc
    13081 ccagctccgc gccttccacc gttgccctac aaatttccgc agccgctttt accgcggtca
    13141 gatgctggcg cagcagcccc ggtttcgccc gtccggctcg aatgccagtg atggtaaatg
    13201 gctggccggt tatcatcgac aggctcagcg ccgagcgcag gatctgcccg ccgccttcgc
    13261 cctgtgcgcc atccagcgca atcatccttt tcatcattta tccttttacg cacaccacct
    13321 gacgcagggt gtagataact tccaccagat cgctttgtgc cgccatcacc gcatcaatat
    13381 ctttatacgc catcgggatt tcgtcgatca cttcggcatc tttacggcat tccacatgcg
    13441 cggtggcacg aatttggtct tccacgctga acagtttttt cgctttagtt cggctcatta
    13501 cccgcccggc accgtggctg cacgaacaga acgactcttc atttcccagc ccacggacga
    13561 taaagctttt tgctcccatc gaaccgggaa taattccata ttgaccagca cgcgcagaca
    13621 ccgcaccttt acgcgtcacg tagatctctt caccaaagag ctgttctttt tgcacatagt
    13681 tgtggtgaca gttgatctct tccatcgcca gcgtttgtgg ctgtctgacc gttttctgcg
    13741 taatgctctg caacgccgtt accacatttt ccatcatcgc atcgcggtta aggctggcga
    13801 aaagctgcgc ccaggccacg gctcttaggt aatcatcaaa gtattccgta ccttccataa
    13861 agtacgccag atcacgcgac ggcaacgtct caagttggtc ctgcatctct ttttgcgcca
    13921 gatcgataaa gtaagtcccg atggcgttac caattccgcg tgaaccggag tgcagcataa
    13981 tccacacctg ctccgactca tcaaggcaga tttcaataaa gtggttaccc gttcccagcg
    14041 ttcccaggtg tttatagttg ttagtgttaa ggaaacgcgg atatttttgc gttaaccact
    14101 gataaccggc ctccagctca gcccatttcg catcgacgtt aaaaggcggg ttttcccatg
    14161 cgcctttatc acgtttacaa cgcccggtag tacgcccgtg tggtacggcc gtttcaatcg
    14221 cttgacgcag ctccgccagg ttttcaggca ggtctgccgc cgttaacgcg gtacgcagcg
    14281 cgttcattcc acagccaata tccacgccca ccgccgccgg aataatcgcc cctttggtcg
    14341 ggatcacgct accaatggtg gaacctttac ccaggtgtac atcaggcatt accgcaatat
    14401 gtttgaaaat aaacggcatc ttcgccgtat taataagttg ctgacgcgca tcggcttcta
    14461 ccggcacgcc tttggtccac atttttaccg gggcattttc agtggtcagt aattcgtaat
    14521 tcattttgtt tttctctttt cgttgtttgc ggtcctgata attgcaaccg tcgtgccaga
    14581 aaattaaata agcagattaa tttattgttt ctaaaaagat tatatcttta tatccgtaaa
    14641 cggagatttc ccgcaaagcc gatttaccga taatgaaata tcgcctttta taaggatatc
    14701 taagatgcgt aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg
    14761 cggcagtcag cgctggtcaa aatggcgtcc gtcactctgt atataccagc aagaatcgtt
    14821 ggtcatcgat cgactggaat tattgcacga tgcccgctcg cgctcgctat ttgaaacgct
    14881 taaacgcgat atcgccagcg tttcgccaga aacagaagtg gtgggcgttg agattgaact
    14941 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
    15001 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
    15061 gcagatttgc tggtttctgc tggcggaagc gcgttatcta cccgcccggt tgatacaatc
    15121 ttcaactccg cgcaaaaaag aacagccgcg cggcccaggt gaagtgacga ttatcgatct
    15181 cgatttaagc cgttataacg ccatcgccag ccgctttgcc gaggaacggc agcaaacgct
    15241 cgatttcctc aaatccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca
    15301 gatcgaaaaa gtggcgatta aatcccgcgc gccgattctg cttaacggcc cgaccggcgc
    15361 aggtaagtca tttctggcgc gacgcatctt cgagttaaaa caggcacggc atcagtttag
    15421 cggcgcgttt gtggaagtga actgcgccac cctgcgcggc gataccgcga tgtcgacgct
    15481 gtttggtcat gtgaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gattattacg
    15541 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca
    15601 ggcaatgctg ctgaaagcta ttgaagagaa aaccttttac ccgtttggca gcgatcgcca
    15661 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc
    15721 cgaaggcaaa ttccgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc
    15781 gggtctacgc cagcgccagg aagatattga accgaacctg gattatgaag tggagcgcca
    15841 cgccacactc actggtgaca gcgtgcgttt taacaccgaa gcgcggcgtg cctggctggc
    15901 ctttgcaaca tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt
    15961 cacgcggatg gccacctttg ccactagcgg acgcatcact ctggacgtgg tggaagatga
    16021 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
    16081 gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc
    16141 tatctgccgc caggcaaagt cgctttctgc tgccggacga cagctttttg atgtttcgcg
    16201 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
    16261 tctgacgtgg gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag
    16321 cacctgcatc acgcttactg gcggcggggc gtcggtgtag acggcatcaa ccatgctgat
    16381 gctccccata ttgaccatcg cattacggcc aaattttgaa tggtcaacca ccagcataac
    16441 gtggcgcgag ttctcaataa tggcgcgctt ggtacgaact tcgtggtaat cgaactccag
    16501 cagcgagccg tcgctgtcga tgccgctgat ccccagaatg ccgaaatcaa ggcggaactg
    16561 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctac gtaattcgcc
    16621 accggcgaga ataatgcgga aatcttcttt taccatcaac gtgttagcaa cgttgagatt
    16681 gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt
    16741 ggtgccgata tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc
    16801 gatgcgctct ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga
    16861 actggaaggc agcgccgcgc cgccatgatg gcgcaggatc aggttttgct ctgccagctc
    16921 gttgaggtcg cggcgaatag tctgcgggct gacggagaag tgctccacca gttcctcggt
    16981 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
    17041 catttataaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
    17101 tcgctaaacc cacggctaat ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
    17161 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
    17221 aaatgccact ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca
    17281 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcata tagccgctta
    17341 acagggcgct gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg
    17401 cgccgccgag ataccaccac caaagcaggt taaagaggat atgcatcagc gagaagtgca
    17461 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg
    17521 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc
    17581 aggcgatcat catcacccag gtcaccggac ctgcgcgttc acgcaaggca gcaaagaaag
    17641 gataacggcg ataatgcagg ccactgccgg tatggcctgc cagccagctg gccgccagat
    17701 aacgcggatc tgccgggttt tcgagaaaac gcgccagctc cgcccgcacg cgctcggcct
    17761 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
    17821 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
    17881 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
    17941 gccgtacgcc acctctgcgg gaaactgtcg ctgccaggct tcaaagccgc cgtcaatgct
    18001 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcccctttgc tgctattgcc
    18061 gtgataacac atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag
    18121 cgtgtcgttg gttaaatgga aagcctgcac cgcatgcccc atagcgaaac tctgtggatc
    18181 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
    18241 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
    18301 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
    18361 gttttttcaa tgttacccaa agcgcgattc tttgctaata tgttcgataa cgaacattta
    18421 tgagctttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
    18481 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
    18541 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
    18601 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
    18661 cgtgaagtgc tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca
    18721 catcgtccgc atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat
    18781 ctgggtaaac gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg
    18841 ttaaaaccgg aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt
    18901 ctggtactcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact
    18961 cgcgccacct ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
    19021 accggcaaaa aatatagctg gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg
    19081 aaacagttct tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
    19141 ggcagccata ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac
    19201 gaagataaac gcattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc
    19261 accgatgtgg aatacaaagg tgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat
    19321 tacctgctga aagtgtataa cgcgcacttt aaaaagcagc taagccgtga cgatatcgtc
    19381 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaagccatt
    19441 acccgtgatt acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta
    19501 ttcggcggta agctgaccac ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg
    19561 ccgtattatc agggtattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc
    19621 attgaaggcg accgcgacga ttacgccgct cgcctgcgcc gccgttaccc gttcctgact
    19681 gaatcgctgg
//
LOCUS       DAJTRM010000078        19591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRM010000078 DAJTRM010000000
VERSION     DAJTRM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.078"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            338..1066
                     /gene="mpaA"
                     /locus_tag="OO322_002898"
     CDS             338..1066
                     /gene="mpaA"
                     /locus_tag="OO322_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5907704.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1041..2006)
                     /gene="ycjG"
                     /locus_tag="OO322_002899"
     CDS             complement(1041..2006)
                     /gene="ycjG"
                     /locus_tag="OO322_002899"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5907705.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2125..2631
                     /gene="tpx"
                     /locus_tag="OO322_002900"
     CDS             2125..2631
                     /gene="tpx"
                     /locus_tag="OO322_002900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5907706.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2675..4216)
                     /gene="tyrR"
                     /locus_tag="OO322_002901"
     CDS             complement(2675..4216)
                     /gene="tyrR"
                     /locus_tag="OO322_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5907707.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4364..5425)
                     /locus_tag="OO322_002902"
     CDS             complement(4364..5425)
                     /locus_tag="OO322_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5907708.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5422..6819)
                     /locus_tag="OO322_002903"
     CDS             complement(5422..6819)
                     /locus_tag="OO322_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5907709.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6975..7973
                     /locus_tag="OO322_002904"
     CDS             6975..7973
                     /locus_tag="OO322_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5907710.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8084..8956)
                     /gene="ompG"
                     /locus_tag="OO322_002905"
                     /pseudo
     CDS             complement(8084..8956)
                     /gene="ompG"
                     /locus_tag="OO322_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(9029..10110)
                     /locus_tag="OO322_002906"
                     /pseudo
     CDS             complement(9029..10110)
                     /locus_tag="OO322_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(10124..10783)
                     /gene="pgmB"
                     /locus_tag="OO322_002907"
     CDS             complement(10124..10783)
                     /gene="pgmB"
                     /locus_tag="OO322_002907"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5907711.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(10780..13047)
                     /gene="ycjT"
                     /locus_tag="OO322_002908"
     CDS             complement(10780..13047)
                     /gene="ycjT"
                     /locus_tag="OO322_002908"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5907712.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(13044..13667)
                     /locus_tag="OO322_002909"
     CDS             complement(13044..13667)
                     /locus_tag="OO322_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5907713.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(13664..14086)
                     /locus_tag="OO322_002910"
     CDS             complement(13664..14086)
                     /locus_tag="OO322_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5907714.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(14108..14883)
                     /locus_tag="OO322_002911"
                     /pseudo
     CDS             complement(14108..14883)
                     /locus_tag="OO322_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(14901..15953)
                     /locus_tag="OO322_002912"
     CDS             complement(14901..15953)
                     /locus_tag="OO322_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5907715.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(15984..16826)
                     /locus_tag="OO322_002913"
     CDS             complement(15984..16826)
                     /locus_tag="OO322_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5907716.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(16813..17694)
                     /locus_tag="OO322_002914"
     CDS             complement(16813..17694)
                     /locus_tag="OO322_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5907717.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17715..18998)
                     /locus_tag="OO322_002915"
                     /pseudo
     CDS             complement(17715..18998)
                     /locus_tag="OO322_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(19012..>19591)
                     /gene="ycjM"
                     /locus_tag="OO322_002916"
     CDS             complement(19012..>19591)
                     /gene="ycjM"
                     /locus_tag="OO322_002916"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5907718.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         4722 a         5427 c         4766 g         4676 t
ORIGIN      
        1 gcatcaatca acccgggact gatggcgata agccattctt catgtaaatc aattatatag
       61 ataccatcgt actgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc
      121 aatcagatgc gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca
      181 ttacgacaat tctaatatct gcacatcaat gaaaatgcaa acaacttccg gcaaaaaagt
      241 gatacaagat cgatgacata gcgttgaaga aaaggatttg ataatccgca aaatttgcac
      301 aacactgcct gtaatacttc ctgaaccaga gacgatcatg accgtaaccc gctcacgcgc
      361 cgaacgcggc gcatttccgc ccggaacaga acattacgga cgttcattat tgggcgcgcc
      421 gttaatctgg tttcctgccc ctgcagccag ccgcgaaagt ggtttgattc tggctggcac
      481 tcacggtgat gaaaactctt cggtcgtcac gctctcctgt gctctgcgga ctttgacacc
      541 ttctttgcgc cgccatcatg tggtgctgtg tgtgaatcct gacggctgcc agctggggtt
      601 acgggccaat gctaatggtg tggatttaaa cagaaacttt ccggcggcga actggaagga
      661 aggtgaaacg gtttatcgct ggaacagcgc cgctgaagaa cgtgatgtcg tattactgac
      721 aggtgacaaa cctggctccg aacctgaaac ccaggcgctg tgccagctta tacaccgcat
      781 tcaacctgcc tgggtggtct cattccacga tccactggct tgtattgaag atcccagaca
      841 caacgaatta ggcgaatggc tggcccagtc gtttgaattg cctctggtaa ccagcgtcgg
      901 ttatgaaacg cctggctctt tcggcagttg gtgtgcagat ctcaacttgc attgcatcac
      961 cgcagagttt ccgcccatct cctccgacga agccagcgaa aaatatctct ttgcgatggc
     1021 aaacttgctg cgctggcatc ctaaagatgc aattcgcccg tcgtgaactg aagcgccggt
     1081 tccacatcta ccgccagcca ggtcggtccg tcaagatcgg cgaaactgac ctgcggcacc
     1141 agcggtaaag cggcgctaat ggcacgagag gtacacaaca tgcagcccag catcagactg
     1201 aaaccttgtg cacgcgcttc agtcgccagc gccagcgctt ccgtcagacc accggtttta
     1261 tcgagcttaa tgttaaccat ctcatagcgc cctttcagcg ccttcagatt gctacgagta
     1321 tgacaacttt catcagcaca aatcggcaac ggatgaataa aattctccag cgccgcatcg
     1381 tcctgcgcag gaagcggttg ttcaagcatc gcaacgccta aatccgccaa tagctggcaa
     1441 cgcgccgcca acccttctgc acgccaggat tcatttgcat caacgatcag cgtggcatcg
     1501 ggcacagcgg tgcgaattgc caccatccgc tcactgataa gatggttatc cagcttcact
     1561 ttcagtaatt tcgcgcctgc ctgccagagt gttgatgcac tattggccat ctgatcaggc
     1621 gtaccgatga caaccgtctg tgcagtgata actgtctcgg gaagcgttat gccgatcaaa
     1681 tcagccagcg attgctgctg ttttcgcgcc gcgagatccc acaatgcaca atccagcgca
     1741 ttacgtgctg cgccggcagg gagaattttt tgcaactcct cccgtgtcag ccctttctct
     1801 aattgcggca cgacgctcat aatttgcgcc attaccgagg catcactttc tccataacgc
     1861 ggatacggcg tgcattcgcc ggtgccttta ataccctctt cttccagttc aaccaccacc
     1921 acgcgcgctt cactgcgact tccccgggca atcacaaacg gggtatgtaa gggccaggct
     1981 tcctcaaata ccttaacgat tctcatcttt actccttgtt gtcgcggtgg tctgcggatt
     2041 aagcgcaaat agcgtttgct gtgttattga cagttagcat aaactaggtg tgacgttaac
     2101 tatatgtaaa caggaaagat aatcatgtca caaaccgttc atttccaggg caacccggtt
     2161 acagtcgcca attccatccc gcaggcgggt agcaaagcgc agacttttac tctcgtggca
     2221 aaagatctgt ctgacgtcac cctcggtcag tttgcgggta aacgcaaagt gctgaacatt
     2281 ttcccgagta ttgataccgg tgtttgcgcc gcatcagtac gtaagtttaa ccaactggca
     2341 actgagatcg acaacaccgt tgtgctgtgt atctctgccg atctgccgtt cgcccagtct
     2401 cgtttctgcg gcgcagaagg tctgaacaac gttatcaccc tctccacttt ccgtaacgct
     2461 gaatttctgc aagcctacgg tgtggcaatt gctgatggcc cactgaaagg tctggcagcg
     2521 cgtgccgttg tggttattga cgaaaatgac aatgtgattt tcagccagct ggtggatgaa
     2581 atcaccaccg agccggatta cgaagcagct ctggctgtac tgaaagcata agttaatatg
     2641 cctgatggtg ttgcaccatc aggcatattc gcgcttactc ttcgttcttc ttctgactca
     2701 gaccatattc ccgcaactta ttggcaatcg cggtatgtga aacgccgaga cgttttgcca
     2761 gtttgcgcgt gctgggataa ttgcgataaa gctgggttaa taccgagcgt tcaaaacggc
     2821 tggtgatttc gtccagcgaa ccttccatcg catcttcgcc cacggctacc gttgcggcgt
     2881 cataatccgg caacaaaata tcctgtggac gcagctcata accgtccagt tgtgtcagtg
     2941 cgcgatagat agcgttcttt aactgccgca catttcccgg ccacgcataa cgtgtaagta
     3001 cagtattcag gtcagcggcc agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg
     3061 cgacgaacag ctcagttaac ggcataatgt cctgcggaca gtcacgtagc ggcggcagat
     3121 tgagcgtcaa cacgttcaga cgataataga gatcttcacg gaacacgcct ttttgcacca
     3181 gttcgaccag attcttctgc gtagcgcaaa tcacccgcac atcgacatgc acctcatggt
     3241 cttcgccaac ccgacggaaa gtgccatcat taagaaaacg cagtaatttc gcctgcatcc
     3301 gtggtgacat ttcccctatt tcatccaaca gcaccgaacc accgttcgcc tgctcaaaga
     3361 accctttctt cccttccgga gcatgaccaa acagttcact ctcgaccgca tcttctggta
     3421 tagacgcgca attcagcgcc aggtaaggtt tgcccgctct ggggcttgcc tgatggcagg
     3481 cgtaggcaaa aagatcttta cctgtacctg tgtcacccgt aatcagcagc ggcgcgctta
     3541 gcatcgccag tttctgcgcc tgttcgacaa cgtgcttcat tttcgggctg acggcgacaa
     3601 tttggctgaa ggcgctgacg tcctgggcgg cgacattttg caactggcgg cccatacgaa
     3661 tcgttgatcg caacatcacc accgcaccgg tcaggacgtg ttgatcattt tcatcctgaa
     3721 gataaacagg cgtaatctcc atcaggaaat tctgcccatt aataacgaca tgctcgttat
     3781 gcgaatcttg cggttcgctt tccagccaac gtaaaaaatt aaagccgtta atcaattgtg
     3841 cggcggtatg gttgcgcagg cgatccaatt tttgcccaaa aagctgacag ctcgccgggt
     3901 tcgccatatc cactttgctt ttcatatcga cagagagcac aggttcaggc aacgcctcca
     3961 gtaacgcgct caacgccaga tgctcacgtt cggaaggcat ccacgggaca gtacgcacat
     4021 cggtaacacc tgcaatacgg cgtatttcgg ccatcagact gctgaaactc tcaaactcca
     4081 gttcagcaaa attgaggtag attcgcccaa tgggatcaat ctcaataccg cgtaaatcaa
     4141 tgcctcttag cacgagtaga tcgagtaatt cgcgggtcag accgagtcgg tcttcacaaa
     4201 agacttccag acgcatggga accttcacct gaaaaaagaa caattaatat gatgatatga
     4261 cactatttgg atagcaggaa ggtttctgtc aacaatcatt gacacaaaga cggaaaaacg
     4321 ggcacttcgg cgtaaagata tccggtcagc acccgatatt gcattatttt tcgctgggcg
     4381 ttttgccttt ttgcagagtt tctttcacct gaccgataag ctgacgacgg aaatccccaa
     4441 ggcgaggttt gtcatcgtca atccacggca gcgggcggca aagctccata gctttaatcc
     4501 cgagtcgtac cgtcagaagt ccagcaccaa ttccctgagc tgcgcgggta gacaaacgag
     4561 cagcgagatc ttgcgacatc cagtccatcc ccacttcgcg caccagttcg ctggctccgg
     4621 caaaagcgat attcagcaat accagcttaa acagacgcaa acggctgtaa taccccagtt
     4681 caatgccata cagcgtggca atgcgattaa ttaaacgcag attgcgccag gcgataaacg
     4741 ccatatcgac caacgccagc gggctgaccg caatcatcaa cgttgattcc gccgccgaac
     4801 ggctgatttc gcgccgcgcc tgggcatcta aaactggctg gaccaaatgc gcatacaggc
     4861 tgaccacttc acgatcgttc tgcgtttcat ggattgaggc ataccagcgt tgcagcgccg
     4921 gatgcgactg atcaataccc gcctgctgcg ccagtttttc gcaaaatgcg cgacctttgc
     4981 ccgtgccgtg gctatgcaat aaatcacgcg cttcgtcgcg ttcatgggcg cgctgtcgca
     5041 agcgccataa gcgccgccac tctgttacca cagaacctac gccagcgccg ataatcaatg
     5101 ccccagcggc acatccaccc agcgctaccc agtcctgggt ttgccaggca ttcattgtcc
     5161 actgtatacc ctgcccgaca acgcttgcgc caaacagagc cagcccgccc atcaccattt
     5221 tgcgccacag gctgcgtttc ggacgtaacg ctgcgtccat taccgcttcg acttgcccct
     5281 cttcttcctg cgcttcgtcg agcgtggccg gggcaaaatt ttgcgcctga ttttcgtcaa
     5341 aggtctgctg cgccctgaat tttggattct gttcgacctc cagcggaccg tcgaaatcaa
     5401 tacgtggttt taacggttcg gtcatcgcaa tttatctcct attaaaaatt ccagcgcggc
     5461 atcaagacga atatgcggca gcggtttgtc gacatccatc acctgcgggc gaaacgcttc
     5521 aaactggaac ccttgcttgt cccagaacgc ctgaccgggc aaacgcgcag gaacttcacc
     5581 ggggtaaacg gtgagaagcg caccatcgct gagccggtta ccgcgcaatg ccggaatttt
     5641 ctcgccgttg acgtcaataa tgccgctggt ggtcgcctgc accgaggcca gccccaggca
     5701 atccatacta atcctttcaa acgccgcatt ttgccaggca tcctgaatca gttgttgcag
     5761 taatgaaacc atattggcgt gctgatcgac cgttacatgg tccgctttca tcgcggcaaa
     5821 cagcagtttg tcgataaccg gcgagaataa gcggcgaaac agcgtgcgct gcccgtagtg
     5881 gaaactttgc atcagctgcg tcagtgccag acgcatatca ttaaatgcct gtggcccact
     5941 gttgagaggt tgcaggcaat ccaccagcac aatctggcgg tcaaagcgca gaaaatgatt
     6001 cttatagaac cccttcacca ctttctcgca gtaataatta aaccgctcgc gcagcattcc
     6061 ggcattggtg tgcttatcgg cctgcgccag tttggactcg tcccaggcat cgacatccgg
     6121 ccacgggaag aattgcagcg cgggcgcacc tgccatatct cccggcaaga caaagcgccc
     6181 aggctgaata aagtgcagcc cctgctgttt acagtggtgg agataatcgg tccacacggc
     6241 ggcaatgtct gccagacggt tttcgtcggc aggcgctagc gggtcaagtc cttcgcacat
     6301 cattcgccat ttcgccgacc attcgccgcg ctgaccattg agtaagcccg tcatctggcg
     6361 cgaccagctt aagtaatcct gcgccagcat cggcaggtcg agcaaccatt cgccgggata
     6421 atccacaatt tccagataca gcgtggaggt atccttaaag tggcgcagca gcgcatcgtt
     6481 cgatttaaaa cgtaacgcca ggcgaatttc actgacgccg cgcgtaggcg tcggccaggc
     6541 gggaggatca ccatataact gcgccagtcc ttcatcatag gtgaagcgcg gaatgccaaa
     6601 gtcacgctgg ggaatgcgtt ttacgcccag caagcgctct tcacgcaccg cgcttaacag
     6661 cggcaaacgt gctccggcat gaatattgag caactgatta accatcgcgg tgataaacgc
     6721 tgttttgccg ctgcggctaa gtccggttac agcgaggcgc agatgtctgt cgacaccccg
     6781 attcaccagc gcattgagtt catttttaag tcgcttcatt gctgtcctgt gctgctctga
     6841 agaccagaaa aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca
     6901 actgcccgtg ttgtcatcga cgggcgaact ccataagatg tgcagcattc acgttcggac
     6961 ggagttcctg acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa
     7021 aatccaccgt atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa
     7081 aagtgttacg ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga
     7141 cctcttcggg ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg
     7201 gtaatccgtt tttctctgaa gttttgcatg ccatcaccgc caaagcggaa gaagaaggtt
     7261 tcgacgtgat attacaaacg tcgcacaatc cggcagaaga tctgcaaaaa tgcgaaagca
     7321 aaattaaaca gaaaatgatt aaaggcatta ttatgctcag ttcgccagcg gatgagtcat
     7381 tttttgccca actcgataaa tacgatattc cggtggtggt aattggcaaa gttgaaggtc
     7441 aatatgccca tgtttattct gtcgataccg ataattttgg tgacagcatt gcgttgaccg
     7501 atgcattaat tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc
     7561 atgtttcggt tgatcgcgtt aatggctata agcaaagtct gtccgcgcat aatattgcag
     7621 tgcgtgataa atggattgtt gacggcggtt atacccatga aacagcactg caagcagcac
     7681 ggcaattatt aagtcagtca ccgttgcctg aggcagtgtt tgccactgac agcctgaaat
     7741 taatgagcat ttatcgtgcg gcggcagaga aaaatattgc tattccgcag cagttagcgg
     7801 tggtgggtta tagtaatgaa acgctgtcgt ttattttaac gcctgcacca ggcggcatcg
     7861 atgttccgac gcaggagtta ggtcaacaaa gctgcgagtt attattccgc ttaatttcgg
     7921 gaaaaccgtc accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag
     7981 ctaccttgcc tgaatgccgg atgcgatgct ggcgcatctt atccggccta catttataga
     8041 tcggataaag tactcgcgcc gcatccggca atttagccca ttatcagaac gagtaattta
     8101 cgccgacacc tgcataatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa
     8161 acgcgtattc cagcgaaacg gaaagtccgt tctggaaatc ataaccgtaa aataaaccca
     8221 cacggttaaa atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactctagc
     8281 gatccagccc aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat
     8341 aggcgcgaat ttcttgtgtg gaaaactcac cgttattgcg gctgtcgtcc atattgaagc
     8401 cgcgctcgag ataatagttc actcgcaaag caaccgtttc gttgaaggta tattgcagac
     8461 ctgtttccgt ttcgacacgg gtatcagcgt aaccggtcgt gttcaaatcg ttggcaaatt
     8521 tatacatcga caaccaaccg ttgaaacgta aatcatcagt cagtttcaca tcccagtctg
     8581 gcgcgatttt ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat
     8641 aaccataatt acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact
     8701 gataatgcac ctccagctcc gggcgatcaa acctcgttcc acgtttaccc gcactataat
     8761 ctaccggccc ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa
     8821 aatagactga aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta
     8881 tttcgtacat cgcaccgata ttaaagtgcc agtcgctctt ttcctcggcc tgtgcgcagg
     8941 ccattcccgc acacatcacc agtgcggtac agggtaataa ctttttttgt tccttgtatt
     9001 atcctggggg aatgccttcc ccctgtattt agcgaattgc tatttccgtt tctgcatcaa
     9061 agaaatgaca tttcgtcata tcaaaatgaa tagtgatatt ttctcctgca tgataatcat
     9121 ttaacgcacc agcacggacc actaactcgt gcccccaacc gtggtgtaga gcataaattc
     9181 cgcaccggtt aattctgcca cgctaatttt ggcggaaatg ttattttcct cttgcgcgtc
     9241 cggatgaata tcttccgggc gtattcccat cacgatgggc ttatgcaaac tttcctgtgt
     9301 tttcagaacc gctaatttct cttcgagaat ggttaattta agcgtttccg taacgaattt
     9361 atcgccatcg atcgtgccgc gaataaaatt catcgctggt gatccaataa atccggcaac
     9421 aaacatattc gcgggttggt tataaacggt tttcggcgca cctacttgct gaacaatccc
     9481 gtctttcata atcacaatcc gcgccgccat ggtcatcgct tcggtctgat cgtgggtcac
     9541 gtagatcatg gtggtgttca gtttctgatg cagcttgctg atctctgcac gcatttgtac
     9601 gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg
     9661 cacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt
     9721 gcgtttcagg tactcacgca ggccgagaat ttgcgccgcc cagttcaccc gctcatcaat
     9781 cacctctttg gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt aaaccgtcat
     9841 atgcggatac aacgcgtagt tctggaacac catcgcgata ttgcgtgctt tggctggaac
     9901 gtcattcatt cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc
     9961 aatcatgcgc agggtggtcg atttaccgca gcccgacggg ccgacaaata cgatgaactc
    10021 tttatcggca atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt
    10081 ttgaatatgt tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga
    10141 aggccgataa ccgcgaccag gtcagggagt ccgttgaggg caacagtaat tgcgccccgg
    10201 ttaagcccgc accgatcccc accgagcgca taccgctggc gttaatggcg tcaatgcccg
    10261 cctgcgcatc ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag
    10321 cgagaaagat ttccggatcc ggtttcgagt ttttaagttg ggaagcatcc gcgcagaagg
    10381 tgaaaaactc acgcagctcc agcgccgcta aaatcgtcgg cgcattcagg gagacggaag
    10441 ccagccctac cggaatctgc tgtgcacgga gctctgccag caaattgcga ataccgggca
    10501 gcacagcatt aaccgtcagc tcgcgcagtg agtggacgta aagcagattt ttgcgatacg
    10561 ccagttgcgc ccgctcctgc gggttaaagt cgccctcttt gcccccgtgt tgcagaatgc
    10621 gccgcagaga ctcatcgcgg ctgatccctt ttagagattc gttaaactgc gcatcaatac
    10681 tgatgccaat ttcagcggca atctgctgcc acgcctggaa atgcagatgc gcggtatcgg
    10741 tgattacacc atccagatcg aaaattaccc cttgcagttt cattcatcct cctgatgcgt
    10801 ggtagcggtc ccattgaagg gcaaaataaa atcacccaaa cagaaaacag attcttcagc
    10861 cacggatata agctgaccgt ccaaacgcag tgaaacgggc gcagaagttc gaatcgcaat
    10921 acgctgcgcg tcgagagtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa
    10981 ctgttgccac tgctcaagta acgccggatt tagatgcaat tcaccgtcac gcacgctcac
    11041 cccggcaaaa ccctgaatcg cccccagcca gatagcgcca gttgcggcag catggatacc
    11101 atcatcacaa ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg
    11161 ataactttgg gtcagcaggc cacagcgtgc agcaacaatg ccgtggattg ctttacttaa
    11221 tgacgagtcg tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccga
    11281 tgagaactgc tctggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg
    11341 catctcgttc acttctgcgc gtgaataatc cagcagaatg gtttgcttcc ccgccgccgc
    11401 tttgtatttc gccagattaa tcgccggctt agtcataaac gaatcatcct gcggcaaaac
    11461 accgtcgggc tgaatttctg gcagccgcag ttctttaagg aacatttcgg cacgatgaat
    11521 aaacgcgtcg tcactacagc caaactggcg ggcaatactc agcgcctgct ggacgttgta
    11581 atacaccata tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc
    11641 aataacatca tgaatttcca gacggtcgtt aaccctcacc gcgcggctaa tccagaactt
    11701 tgcagtttcc agaagtagcg ccatgccttc atgcgcaatg aaactttcat cccccgtggt
    11761 ctgccagtat tgaataaccg cccaggcgat atcggccacc agatgatgtt ccgcctgcgc
    11821 cgaggccact ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc
    11881 ttcttcgccg ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg
    11941 cgctttctcc tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac
    12001 cgtcggatcg ctaaacagat ggaacggcag caaaaatact tctgtatccc agaaaacgtg
    12061 gcctttgtag ccttcgccgg ttaagccttt tgccgcaata ctgctgcgct cgccgtgagc
    12121 cggcgtcatg atgcgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc
    12181 gccgccattt accgtgatac ggcgtttctg ccaccattga cgccagtttt ctgttgatgc
    12241 tgcaagatgt tggtcgtaac tctgttgcgc gcacatttca agctggcgaa gcgacacgct
    12301 gccccactcg tctaaagcag cttgcctgtc atcccgccag tcgatccaca ccagtttttg
    12361 caacgtcact gtctcgcctg catgaagctg cgtactggta tgttgcagta aacggcgctc
    12421 tttggcggta tagcattgct gcacatcacc gctcacctgg cagcaacaac tgatggccac
    12481 atcactgcgc ccatcctggg tggtgtagat cccctgcatc aaatgctgac caaacacccg
    12541 cacctgggtt tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt
    12601 tgaaatcagc actgacgcgt cggcgtccag tggcataata gtgatttcca gcgcaatgag
    12661 cggcagttgg tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg cgccgttgct
    12721 ggtacgccag acaacgctac ggcgtaattc tccactggca aagtcaagtt cacgctgcca
    12781 ggcttcgcgg gataacgaga aaacctcacc atttatggca atctccatcc ccaagatatc
    12841 aggcagattc accagttcgt tgatttcacc ttttcccgcc cgatgataca gccccgccag
    12901 atacatccct cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata
    12961 gccgttcccc tgcgccatca gcgatgcata cttgttcagg gtatgctggc tgaaatgggg
    13021 ttctgataac gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga
    13081 tataacgccg ccaccagttg ctggatgatg tacccctgct cggcgtcggc tatcatcacg
    13141 ggcttgccct gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct
    13201 gccatttccc gttgcatcag cgtcattaat tcaccgttgt tatcggtgta gatatgtgct
    13261 ggaaacagcg tcgcaccggc tttatcacca caaaagctga cgttcataat caactgttcg
    13321 cggatgttga gtgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg
    13381 gtgccaaaca gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca
    13441 cagctctttt gcgtgccgat cttttgaaaa ctatgcgcat tcacgctttt caccgccgga
    13501 aagcccagca catacatcgc agcatccagc atatgaatgc cgatgtcgat cagcaggcca
    13561 ccaccctgca gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg
    13621 gcgcgggcgg tggtaacgta aatctctccc aaaacgccgt tggtcacctg ttcacgcagc
    13681 tgttgcgtat cgagcgcaaa cgatggtgaa agtcgtaggc cagcaccttg cccagtttgc
    13741 gcgcggtatc gcacatttcc cacgcctgtt ctgtcgtcat ggcgggcggt ttttcgcaca
    13801 tcacatggca gcctgcttcc agtgccatca gggtatgttc gtaatgaaaa cggttaggtg
    13861 agccgacgct aaccacatca ggtttcaccg ccagcagcat ggcctgcgga tcgtcccaca
    13921 cggatgcatt cccgtatttt tctgccagcg cctgcgcctg ggaaaggtgg ctgtcacaga
    13981 cagccaccag ttccagatcg ttgcgggtgc agtagtacga agcatgaacc ttatccgcca
    14041 cctggcccgc gcctattatc gcgacccgca gcggagagct tgtcattgca cttttcacaa
    14101 agacctctta gcaggtacgc aaccaggcca acgaatcacg gtacgcctgg gcaggatctt
    14161 ctgcccggat acgcccttca tacaccacat aaccctgata gttatcggcg cgcagctgtt
    14221 caaacagcgc gtggaaatcg agggtgccac tgcccggctg gtaacgatgg ttatcggcaa
    14281 tatgcacatg accgagcaag tcacggttgt cgtgcagcgc ctgcgccagg ttatcttctt
    14341 cgatgttcat gtgatagaaa tcgccgataa tctgcacatg tttaagattg ttttcgacga
    14401 tgtaacgacg agcatcggcg agggtgttga tcatatgatc ctgatagcgg tttaacggtt
    14461 cgagatacac cacggttccg gtacgcgcgg cgacctgttc cagtacgcgc agggaatcac
    14521 tcaccagttt gcggtcgccg tccaggctac gcggcgaggt catcggcggt aagcggaagg
    14581 taaacatgcc ccacgctgcc ggaacccccc cacttctgcc agcgcctcga gaatgcgttc
    14641 gatctgcttt aagccattaa gacgacgctc ttcgataaag tcaccaatcc agccgtcata
    14701 gccgccgcag gcggtggtca ctggtaaacc ggtttctttg atcgccgctt tgacttcttc
    14761 gatgttgttg accagcagtt tgccgtcaat ctcaaaacca tcgaagccca tctctttgat
    14821 ataacgaaat ctctcgcgga tattttccgg aaagaatgcc tgattctgtg tgccgatttt
    14881 cattttttca ttcctttgaa ttaaaacgta acgcccattt tgatgctctg ttccggatgc
    14941 tgatcgacat actgcatgta gctttccggg ctgttggcaa aggtcactac cgggtcgatt
    15001 aaatcttcgc aattgagata accgttcatc agcagttccc agcaggtttc ttcaatacgc
    15061 ttacggctcc agcgcggata atccgggttc ggttcgctgc acgcgcggga gaaaacaatt
    15121 ttggcgttat tgaaatgcgc ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca
    15181 aacgcgacat aggagatggt gccgccgtag gccaggccgc gcagcgccga ttgcagcgcg
    15241 tcggcgtaac cgctggtttc gataatcaca tcagccccct gcttgccggt cagcgttttg
    15301 atctctttgc ctacgtcagt gccaatgggg ttaaggcaga aatcagcacc gtggcgacgg
    15361 gcaatatcac agcgatgggc gataggatcg acgccaatta ccaccgatgc gccagcgcgt
    15421 ttcgccagtt ggatggcaat ttgaccgatc gcgccaagcc ctaccaccac gacaaaatcc
    15481 cctacgcgta cgttggcatc gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacag
    15541 acggcgtttt tccaggagct gccttgcggc attttgcgca gcttgtagtt attcactgcg
    15601 ttaatgatga ccgtctcgga gagcgggccg tagccgcata cgctgtcgcc caccgcgtag
    15661 tctgtaacgt cgctgccgca ctcgataatg tcgccaacca ccatgttgcc aagctggaat
    15721 ttgccaaact caatgccgcg cggcgcatct gccggacgcg gggtgaacat ctgccactcg
    15781 ccgttaaagt cttcatcgat aaacgggctg gcggcgcgga agtcgaccac ttccgttccg
    15841 tgtttcggtg cgccgaaacg cacgcggatc ttcacttcat tggctaaaat cgcccgatct
    15901 tcatactcaa ccagcgcagc aacacgcggt gctgtggcta ctaacttttt catgtaaaac
    15961 tccttgtaaa gaaactggcc gctttagccc ttcacgccac cggcggtcaa accactctta
    16021 ataaaacgtt ccaaaagcgc atacataatc accaccggca atgcggtcac cagcgaggcc
    16081 gccatcatcc gtccccagat gtaatcaggc gtactgaaca gcgcgttcag gcccactggt
    16141 aaggtgaaat tgctggcgct ggagaggaaa atcgacgcaa acagatagtc gttccacgcc
    16201 accataaagc agtagacgaa caccgatatc agcccggaca tcgccagggg cacggtgata
    16261 cggaagatga tttgcagacg gttgaggcca tccatcatcg ccgcttcttc gatctcatca
    16321 gggatggtgt cgaagtaact tttcagcata aacacggcgg tgggcagggt ttgcgtcacc
    16381 atggtaatga tcagcgccat ctcggtgtcg taaataccaa gcgcggtgat gattttgaac
    16441 agaggcacca ccagcaaaat gccagagaac atatacaccg tgtaaaagct ggcgttgatg
    16501 gtcatccggc ctttaaagcg caggcgggaa agcgcgtaag ctccgagaat gccgagaaat
    16561 accgccacca cggaggaaac caccgacacc accatgctgt tacggaagta gtcgacaaac
    16621 gggaaaatca tcgggttaaa aatgtcgacg taatgctcca gcgtccactg ttgcggcagc
    16681 agcgtaggat gcagcgatat cgcctctttc gcgcttttga acgaggtcat cagcatcaca
    16741 aaaaacggaa acagcgtgat gatgagaaac agcgccagcc cgcagtaaaa accgatacga
    16801 ctgagcgtgc gtttatttgt tgccattgag gttcacccgt ttcctggtca gcagaatgac
    16861 agcgaaaata atgatgaaga gcaccaccga gatcgccgcc gctttcccta agtcattaaa
    16921 ggcaaaggcg gttttgtaga gatagacacc aagaatatcg accttggttg tcagcagata
    16981 aacatcagcg aacatgtaga acatccagat ggtgcgcagg gtcaccaccg tcgccaggac
    17041 cggcataatt gcgggcagcg tgacgatgcg aaaccgctgc caggcattag cgccatccat
    17101 ctctgccgct tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa acgagataaa
    17161 ggcatacggg aagtagcgcc agatggcgaa cagcaccacc agcacaaagc tactgcccgg
    17221 attatcaaac cacagcggtg cctgctcata gagatgcaga agatcgacgc cgaggtagtt
    17281 cacaatgccg tagccgttgt tgaacatgta tttccaggca aacaccagcg aaatggacgg
    17341 cgttacgtag gagaggatca ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt
    17401 gaaaaacatc gccacggcca gcccgagcac ggtgctgccc gccaccacca gcgccgtata
    17461 ccagaccgtc atccacagcg agtgccagaa gccgggatcg gagagaatgc gcacatagtt
    17521 actcacccca acaaacgttg actcgatgtt gggattgagc ggcagacgta aaaaactgat
    17581 ttcgatattc gacaccatcg gccacgccac cagaccgccc agcagtaata agctgggcgc
    17641 gagaagcagc agcgcaaacg gcatatcgga acgacctgaa aaaagcctgt tcattgcgga
    17701 ttcctgtcct gggtttagtg ctgttcgatc agttcatcca gctttttctg gctcgcttgc
    17761 agcgtgccag ggagatcggc tttacccacg gtgacgttat gcaccatgga gctcaccaca
    17821 ccagaacccg tcacatcgcc catgcgggta aagtttttat cccctactgc gccaaagacc
    17881 tgaatatttg gcagttcacc gattagctga ttcggtagtt cacccagcgc cttaataacg
    17941 tcgttgtctt tccaggtggc ggtagtgaca accgctttat tcaccggcag cgcagcacct
    18001 ggcgacatca tcacccagtc ggcaatgttg tccgcctgct ccataaaggt gacaaatttt
    18061 tctgctgctt cggtcttttg tccggcggta atggtcagcg aggtcaacat gccgtagacc
    18121 gcagagtttt tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatc
    18181 acagccggaa ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg
    18241 tctttcactt ccatgatgtc gttagaaccc ggcatggtgt tggcagcaag gtcgcggtaa
    18301 taggtcagcg cctgcatcat ctctggtgta tcaagggtga ttttgccttc ggcgttaaag
    18361 acgttggcct agttggataa cgcaaactgg gagaaggatt gttccgtcag cacgctttct
    18421 gccgtaggca gcgcaatacc gtatttttta ttcgccgggt cattcagttt ctgtgcaacg
    18481 tccagcagct gttgccagtt tttcggctcc tccagccccg cttttgccag cacatctttg
    18541 cgataccaga tgccgccaat ccaggcgctg acaggaacac cggtccatgc gctaccatct
    18601 tcggtacgca caatacgcag tacgccatcg taaaacgcgc cttcaccaac gttgctgatg
    18661 accgtggcaa ccgctttgcg atcgataagc tgctctttat ccataacttt ggcgtagtca
    18721 tggctggttt cgatcacttc cggcagcgag ccgctacgtg aaagggtgat gactttggtg
    18781 ttatgagcgt cttcttccac gggcacttgt ttaacggtga tacctgggtt ttctttttca
    18841 aaacgggcaa tcaatttgct gataactgcc tgacgctcct gctccaccga agaatgcata
    18901 aattcgatgg atacatgggt tttattttct tctttacagc ctgaaatcag ggcgcaggaa
    18961 accagtgctg ataacagcac gatttttgat ttaatcatga gatgttcctt tttatttaat
    19021 ccacataacc tgccaggggc gcagcgttat ttcattaccc aatatatcaa cttcactaat
    19081 cagatcccga ccatgcagat cagtaatatt tacatgctga atatttttac tgacattaaa
    19141 caatccagtc agacaattac catcagcgtt acttctttga atacgcatta cggatgaatt
    19201 aaccgtatcg atggtaaaat tattatccgg atgaaactcg ttatggctgc gacgaagtgt
    19261 aattaaacgc gacaactcat gatataccgc atgccttaat gtagcttcat cgttcagttc
    19321 tcgtgttatc tcttcactgt gatatttttt acggttaatc gcacggttat atccgagttt
    19381 ttcgacacct gcgtaatcat tacgcgagcc aagaatactt tgaatatata tcgcgggtac
    19441 accggggaaa cttaacaaaa tcgcatgggc aaggataaac ctggtgcaac gttcttcatc
    19501 gctactctcg cggcggctta acgcattcat ataggtgaca ttcatctcat agggactgcg
    19561 cgtgccgtcg ggattatttt tccagtttac t
//
LOCUS       DAJTRM010000079        19079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRM010000079 DAJTRM010000000
VERSION     DAJTRM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.079"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1882
                     /gene="pbpC"
                     /locus_tag="OO322_002917"
     CDS             <1..1882
                     /gene="pbpC"
                     /locus_tag="OO322_002917"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5907719.1"
                     /translation="LQLEWHLSKREILILYLNRAPFGGTLQGIGAASWAYLGKSPANL
                     SYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSREQVKESREEPIW
                     LAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMI
                     VVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPAS
                     LLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNV
                     GLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLM
                     SSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWT
                     GRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRGVICWPGGQSLP
                     EGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVAADCPQAKQEM
                     VNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAA
                     EATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDVGQIATVKFV
                     MQ"
     gene            2032..2463
                     /gene="ndk"
                     /locus_tag="OO322_002918"
     CDS             2032..2463
                     /gene="ndk"
                     /locus_tag="OO322_002918"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5907720.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2613..3767
                     /gene="trmG/rlmN"
                     /locus_tag="OO322_002919"
     CDS             2613..3767
                     /gene="trmG/rlmN"
                     /locus_tag="OO322_002919"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5907721.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4052..5065
                     /gene="rodZ"
                     /locus_tag="OO322_002920"
     CDS             4052..5065
                     /gene="rodZ"
                     /locus_tag="OO322_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5907722.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5092..6219
                     /gene="ispG"
                     /locus_tag="OO322_002921"
                     /gene_synonym="gcpE"
     CDS             5092..6219
                     /gene="ispG"
                     /locus_tag="OO322_002921"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5907723.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6322..7596
                     /gene="hisS"
                     /locus_tag="OO322_002922"
     CDS             6322..7596
                     /gene="hisS"
                     /locus_tag="OO322_002922"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5907724.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7614..8234
                     /gene="yfgM"
                     /locus_tag="OO322_002923"
     CDS             7614..8234
                     /gene="yfgM"
                     /locus_tag="OO322_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5907725.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8245..9423
                     /gene="bamB"
                     /locus_tag="OO322_002924"
     CDS             8245..9423
                     /gene="bamB"
                     /locus_tag="OO322_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5907726.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9541..11013
                     /gene="der"
                     /locus_tag="OO322_002925"
     CDS             9541..11013
                     /gene="der"
                     /locus_tag="OO322_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5907727.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11083..11298
                     /locus_tag="OO322_002926"
     CDS             11083..11298
                     /locus_tag="OO322_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5907728.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11295..12665)
                     /gene="xseA"
                     /locus_tag="OO322_002927"
     CDS             complement(11295..12665)
                     /gene="xseA"
                     /locus_tag="OO322_002927"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5907729.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12827..14293
                     /gene="guaB"
                     /locus_tag="OO322_002928"
     CDS             12827..14293
                     /gene="guaB"
                     /locus_tag="OO322_002928"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5907730.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14362..15939
                     /gene="guaA"
                     /locus_tag="OO322_002929"
     CDS             14362..15939
                     /gene="guaA"
                     /locus_tag="OO322_002929"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5907731.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16034..16573)
                     /locus_tag="OO322_002930"
     CDS             complement(16034..16573)
                     /locus_tag="OO322_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5907732.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16589..17107)
                     /locus_tag="OO322_002931"
     CDS             complement(16589..17107)
                     /locus_tag="OO322_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5907733.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSVVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17418..17609)
                     /locus_tag="OO322_002932"
     CDS             complement(17418..17609)
                     /locus_tag="OO322_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5907734.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17627..17779
                     /locus_tag="OO322_002933"
     CDS             17627..17779
                     /locus_tag="OO322_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907735.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17961..>19079
                     /gene="pdeF"
                     /locus_tag="OO322_002934"
     CDS             17961..>19079
                     /gene="pdeF"
                     /locus_tag="OO322_002934"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5907736.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGI"
BASE COUNT         4499 a         4641 c         5187 g         4752 t
ORIGIN      
        1 gttgcaactg gagtggcatt tgtctaagcg tgaaattctg atcttgtatc ttaaccgcgc
       61 tccgtttggc ggtacgttgc aggggatcgg tgcggcaagt tgggcttatc tcggaaaatc
      121 gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg gtgttaccgc aagtgcccag
      181 ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc gcgcgtaata aagtgctcga
      241 acggatggct gcgcaaggtg tgtggtcccg tgagcaggta aaagagtcaa gggaagaacc
      301 catctggctg gcaccccgac aaatgccgca actggcaccg ctgttttcgc gcatgatgct
      361 cggtaaaagc aaaagcgaca aaatcgttac tacgctggat gccggtcttc aacgacgtct
      421 ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg cgcagctcac tggcgatgat
      481 cgtggttaat cataccgata tgcgtgttcg cggctgggtg ggatcggttg atctcaacga
      541 tgattcacgc tttggtcatg ttgatatggt caatgcgatc cgatcgccag gatcggtgct
      601 caaaccgttt gtttatggtc tggcgctgga tgaaggcttg atccacccgg catcactgct
      661 gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt aactttgata gcggttttca
      721 tggcccgatc aacatgagcg aggcgctggt gcgctcgctg aacttacctg ctgtgcaggt
      781 gctggaagcc tatggaccga aacggtttgc ggcaaagtta cgcaatgttg gattgccgtt
      841 atatttgccc aacggcgctg cgccgaatct ttcactcatt ctcggcggcg ctggtgcaaa
      901 actggaagat atggcggcag cgtataccgc gtttgctcgc cacggcaagg caggcaaatt
      961 gcgcttacag cctgacgatc cgctgcttga acggccttta atgtcgtcgg gggcggcgtg
     1021 gatcattcgc cggattatgg ctgatgaagc gcaacccttg ccggatagtg ccttgccgcg
     1081 cgtcgcccca ctggcatgga aaacgggcac cagctatggc tatcgtgacg cctgggcgat
     1141 tggggttaac gctcgctatg tcattgggat ctggactggc agacctgacg gcacgcccgt
     1201 tgttggtcag tttggctttg ccagtgccgt accattgttg aatcaggtca ataatatctt
     1261 actgtcgcgc agtgcgaatc tgccggaaga cccgcgcccg aactcagtca ctcgtggcgt
     1321 tatctgctgg ccgggcggac agtctttgcc ggaaggtgac ggtaactgtc gccgccgcct
     1381 ggcaacctgg ctgctggatg gcagccagcc gccaacgctg ttactgtcag agcaggaagg
     1441 gattaatggc attcgcttcc ccatctggct ggatgacaaa gggcagcgcg tcgccgctga
     1501 ctgcccgcag gcgaagcaag aaatggtaaa tgtctggccg ctaccgctgg aaccctggct
     1561 gcccgcatca gaacgtcgcg ctgtgcgctt accaccggcc tcgacaattt gcccgccata
     1621 cggtcacgat gcccagctcc ctctgcagct gacaggcgtt cgcgatggcg cgattattaa
     1681 acgtttaccg ggcgcagcgg aagcgacttt gccgttgcaa tcaagtggag gggcaggtga
     1741 acgctggtgg tttctgaatg gcgaaccgtt aactgaacgc gggcgcaacg tgaccctgca
     1801 tttgacggat aaaggtgatt atcaattgct ggtgatggat gatgtggggc aaatcgcgac
     1861 agtgaaattt gtcatgcaat agtctgtttt gtccctgatt gttgctaaaa ctcattttat
     1921 tttaaaaaaa atgttacctt catcaatagt caacggccct gttgctcatt ataatccgcg
     1981 ccatctcgta cgctggtaca gacaacaaca gaacaattta cagaggtaaa aatggctatt
     2041 gaacgtactt tttccatcat caaaccgaac gcggtagcaa aaaacgtcat tggtaatatc
     2101 tttgcgcgct ttgaagctgc agggttcaaa attgtcggca ccaaaatgct gcatctgacc
     2161 gttgaacagg cacgtggctt ttatgctgaa cacgatggaa aaccgttctt tgatggtctg
     2221 gttgaattca tgacctctgg cccgatcgtg gtttccgtgc tggaaggtga aaacgccgtt
     2281 cagcgtcacc gcgatctgct gggcgcgacc aatccggcaa acgcactggc tggtactctg
     2341 cgcgctgatt acgctgacag cctgaccgaa aacggtaccc acggttctga ttccgtcgaa
     2401 tctgccgctc gcgaaatcgc ttatttcttc ggcgaaggcg aagtgtgccc gcgcacccgt
     2461 taataatttc gtaaatgccg cgtgcaaacg tggcatccgt gcgccagaat ttgtacaatg
     2521 cagcgccccc ggacgagcag ccgctcaccg gggcgtttct tttttcaacc ctccaggggc
     2581 cataacgtgt aataacgagg ccggaatagc atatgtctga acaattagtc acacctgaaa
     2641 acgtcaccac gaaagatgga aaaatcaacc tgctggatct caaccgtcag cagatgcggg
     2701 agttttttaa agatttaggt gaaaaaacct tccgcgccga tcaggtgatg aagtggatgt
     2761 atcactattg ctgcgacaac tttgatgaga tgaccgacat caacaaagtg ttgcgcggca
     2821 aactgaaaga ggtggcggaa atccgcgcac cggaagtggt tgaagaacag cgttcatccg
     2881 acggcaccat taaatgggcg atcgccgttg gcgatcagcg cgtcgaaacg gtgtatatcc
     2941 cggaagacga ccgtgccacg ctctgcgtct cttcgcaggt ggggtgtgcg ctggagtgta
     3001 aattctgttc caccgcccag cagggcttta accgcaacct gcgggtatcg gaaattattg
     3061 gccaggtgtg gcgtgcggcg aaaatcgtcg gcgcagcaaa agtcaccggt cagcgcccaa
     3121 tcactaacgt cgtgatgatg ggcatgggcg agccgctgct caacctgaac aacgtcgttc
     3181 cggcgatgga aatcatgctt gatgatttcg gttttggcct gtctaaacgt cgcgtgacgc
     3241 tttccacttc cggtgtagtt ccggcgctgg ataaactggg cgatatgatc gacgttgcac
     3301 tggcaatttc cctgcacgcg ccgaacgacg aaattcgtga cgaaattgtg ccgatcaaca
     3361 aaaagtacaa tatcgaaacg ttccttgctg cggtgcgccg ttatctggag aaatccaacg
     3421 ccaatcaggg ccgagtcact attgaatacg tgatgcttga ccacgttaac gacggcactg
     3481 aacacgcgca ccaactggcg gaactgctga aagatacgcc gtgtaagatc aacctgatcc
     3541 cgtggaaccc gttcccggac gcgccgtatg gacgcagttc gaacagccgt atcgaccgtt
     3601 tctcaaaagt gctgatgagc tacggtttca ccactattgt gcgtaaaacg cgtggtgatg
     3661 atatcgatgc tgcctgtggt cagttggcgg gcgatgttat cgaccgtacg aaacgtactc
     3721 tgcgtaaacg gatgcagggt gaagccatcg atattaaagc ggtctgatag ccattacgcc
     3781 acggttactt tatgtaccgt ggcgtaatct tttatgaatg aagtattaag ttgcaattgt
     3841 ctggtcaata ttaacggtgc gtttttgctg actttaaggc agtatgtaac gatgcaacag
     3901 taagttagcc tcagtgaatg tgggctttgt cacgagcaca cagacggtct tatactgtat
     3961 gataacggtt aacttaacgg atgtttcgcg gtgtgggtga gcatgatggt tcaccggcat
     4021 ctcaattctc atttaaacgt acctgcagcg aatgaatact gaagccacgc acgaccaaaa
     4081 tgaagcactt actaccggcg ctcgcctgcg taatgctcgc gaacaactag gacttagtca
     4141 gcaggccgtt gccgagcgac tttgcctgaa ggtttccacg gtacgcgaca ttgaagaaga
     4201 taaggcaccc gccgatcttg cttcaacatt cctgcgcgga tatatccgct cttatgcgcg
     4261 tctggtacat attccagaag aagaactgct gccagggctg gaaaagcagg ctccacttcg
     4321 ggctgcaaaa gttgcgccaa tgcagagttt ttccctcggt aaacgccgca aaaaacgcga
     4381 cggctggcta atgaccttca cctggctggt gttgtttgtg gttatcggcc tgagcggtgc
     4441 ctggtggtgg caagaccaca aagctcagca ggaagagatc accactatgg ccgatcaatc
     4501 ttcggcggaa ctgagcagta atagcgagca ggggcagagt gttccgttaa atacgtcgac
     4561 aactacagac ccggctacaa cgtccacgcc gccagcgtct gtggatacta ccgcaaccaa
     4621 cacgcaaaca cctgccgtaa ctgcgccagc accagctgtt gatccgcaac agaatgcggt
     4681 tgtttcgcct tcgcaggcaa atgttgatac cgccgcgacc ccggtaccaa cggcagcaac
     4741 aacgccagat ggtgctgcgc cgttgccaac cgatcaggct ggcgtgacca cgccggcggc
     4801 tgatccgaat gcgctggtga tgaactttac tgccgattgc tggctggagg tcactgatgc
     4861 taccggtaaa aaattgttta gcgggatgca gcgtaaagac ggtaatttga atttaaccgg
     4921 ccaggcaccg tataaactga aaattggtgc gccagccgca gtacagatcc agtatcaagg
     4981 gaaacctgtc gatctgagtc gttttatcag aactaaccag gttgcgcgtc tgaccctcaa
     5041 tgccgaacaa tcaccggcgc agtaacagac gggtaacgcg ggagattttt catgcataac
     5101 caggctccaa ttcaacgtag aaaatcaaca cgtatttacg ttgggaatgt gccgattggc
     5161 gatggtgctc ccatcgccgt acagtccatg accaatacgc gtacgacaga tgtcgaagca
     5221 acggtcaatc aaatcaaggc gctggaacgc gttggcgctg atatcgtccg tgtctccgta
     5281 ccgacgatgg acgcggcaga agcgttcaaa ctcatcaaac agcgggttaa cgtgccgctg
     5341 gtggctgaca tccacttcga ctatcgcatt gcgctgaaag tagcggaata cggcgtcgat
     5401 tgtctgcgta ttaaccctgg taatatcggt aatgaagagc gtattcgcat ggtggttgac
     5461 tgtgcgcgcg ataaaaacat tccgatccgt attggcgtta acgccggatc gctggaaaaa
     5521 gatctgcaag aaaagtatgg cgaaccgacg ccgcaggcgt tgctggaatc cgccatgcgt
     5581 catgttgatc atctcgatcg cctgaacttc gatcagttca aagtcagcgt gaaagcgtct
     5641 gacgtcttcc tcgctgttga gtcttatcgt ttgctggcaa aacagatcga tcagccgctg
     5701 catctgggga tcaccgaagc cggtggtgca cgcagcgggg cagtaaaatc cgccattggt
     5761 ttaggtctgc tgctgtcgga aggcatcggc gacacgctgc gtgtatcgct ggcggccgat
     5821 ccggtcgaag agatcaaagt cggtttcgat attttgaaat cgctgcgtat ccgttcgcga
     5881 gggattaact tcatcgcctg cccgacctgt tcgcgtcagg aatttgatgt tatcggtacg
     5941 gttaacgcgc tggagcaacg cctggaagat atcatcactc cgatggacgt ttcgattatc
     6001 ggctgcgtgg tgaatggccc aggtgaggcg ctggtttcta cactcggcgt caccggcggc
     6061 aacaagaaaa gcggcctcta tgaagatggc gtgcgcaaag accgtctgga caacaacgat
     6121 atgatcgacc agctggaagc acgcattcgt gcgaaagcca gtcagctgga cgaagcgcgt
     6181 cgaattgacg ttcagcaggt tggaaaaata ataacgtgat gggaagcgcc tcgcttcccg
     6241 tgtatgattg aacccgcatg gctcccgaaa cattgaggga agcgttgagg gttcattttt
     6301 atattcagaa agagaataaa cgtggcaaaa aacattcaag ccattcgcgg catgaacgat
     6361 tacctgcctg gcgaaacggc catctggcag cgcattgaag gcacactgaa aaacgtgctc
     6421 ggcagctacg gttacagtga aatccgcttg ccgattgtag agcagacccc gctattcaaa
     6481 cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga tgtacacctt tgaggatcgc
     6541 aatggcgaca gcctgactct gcgccctgaa gggacggcgg gctgtgtacg cgccggcatc
     6601 gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt atatcgggcc gatgttccgt
     6661 cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc agttgggctg cgaagttttc
     6721 ggtctgcaag gtccggatat cgacgctgaa ctgattatgc tcactgcccg ctggtggcgt
     6781 gcgctgggta tcttcgaaca cgtaactctt gagctgaatt ctatcggttc gctggaagca
     6841 cgcgccaatt accgcgatgc gctggtggca ttccttgagc agcataaaga aaagctggac
     6901 gaagactgca aacgccgcat gtacactaac ccgctgcgcg tgctggattc aaaaaatccg
     6961 gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg attatctgga cgaggaatct
     7021 cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg cggggatcgc ttacaccgta
     7081 aaccagcgtc tggtgcgtgg tctggattac tataaccgta ccgttttcga gtgggtgact
     7141 aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc gttatgacgg tcttgtggaa
     7201 caactgggcg gtcgtgcaac accggctgtc ggttttgcga tgggcctcga acgtcttgta
     7261 ttgttagtac aggccgttaa tccggaattt aaagccgatc ctgttgtcga tatatacctg
     7321 gtggcttcag gtgctgatac acaatctgcg gctatggcat tagctgagcg tctgcgtgat
     7381 gaattactgg gcgtgaaatt gatgaccaac cacggcggcg gcaactttaa gaaacagttt
     7441 gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc tgggtgagtc tgaagtggct
     7501 aacggcacag cagtagtgaa ggatttgcgc tctggtgagc aaacggcagt tgcgcaggat
     7561 agcgtagccg cgcatttgcg cacgttactg ggttaaggaa ggagaaggac agcgtggaaa
     7621 tttacgagaa cgaaaacgac caggtagaag cggttaaacg cttttttgct gaaaatggca
     7681 aagcactggc tgttgggggg attttgggcg ttggcgcact gactggctgg cgctactgga
     7741 acagccatca ggttgattct gcacgctccg cttctcttgc ctatcaaaat gcggttaccg
     7801 cagtgagcga aggcaaaccg gatagcatcc cggcggcgga aaaatttgct gctgaaaata
     7861 aaaatactta tggtgcgctg gcttctttgg aacttgcgca gcaatttgtt gacaaaaatg
     7921 aactagagaa agctgccgcc cagttacaac aggggctggc agacacgagc gatgaaaatc
     7981 tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt acagctcaag caggctgatg
     8041 ccgcgctgaa aacccttgat accatcaaag gtgaagggtg ggctgccatt gttgccgact
     8101 tgcgtggtga agcattgctg agcaaaggtg ataagcaagg tgcgcgtagt gcatgggaag
     8161 caggcgtgaa aagcgatgtt actccggcac tgagcgaaat gatgcagatg aaaattaata
     8221 atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat tactgctgcc aggactgctt
     8281 tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg aagaagatgt ggtaaagatg
     8341 tccccattgc caaccgttga aaaccagttt acgccgacca cggcgtggag cacttccgtt
     8401 ggtagcggca ttggcaactt ctattccaat cttcatccgg cactggcgga caacgttgtc
     8461 tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg cggatgatgg caaagaaatc
     8521 tggtctgtca gcctggccga gaaagatggc tggttctcta aagagcctgc attactttct
     8581 ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca gcgaaaaggc gcaggtttac
     8641 gcgctgaata ccagcgatgg tactgtggca tggcaaacta aagtcgcggg tgaagcactt
     8701 tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca ccagtaacgg tcagttacaa
     8761 gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta acctcgatat gccttcgctc
     8821 tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg cggccgtcgt gggtggcgat
     8881 aatggtcgcg tcagcgcagt gctgatggaa cagggccaga tgatttggca gcagcgtatt
     8941 tcccaggcga ccggttctac cgaaattgac cgtctgagcg atgttgacac gactcccgtc
     9001 gttgttaacg gcgttgtttt cgcgctggcc tataatggta acctgacggc gcttgatctg
     9061 cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg tgaatgattt catcgtcgat
     9121 ggcaatcgca tctatctggt cgatcaaaat gaccgggtga tggcgttgac cattgatggc
     9181 ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc tgctgacttc tccggtgctg
     9241 tataatggca acctggtggt cggtgacagt gaaggttatc tgcactggat taacgtcgaa
     9301 gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg gtttccagac tgaaccggtt
     9361 gccgctgacg gcaaactgct gatccaggca aaagacggaa ccgtgtactc tattacacgt
     9421 taatcgtctc tgtcgttcac tttgaaaacg gctcctggac aggggccgtt ttcctgtttt
     9481 taacaacgac gcgaatatag cgtgcgttgt ctgatgattt ataaaaatga ggctttaaac
     9541 atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag gaaaatccac gttatttaac
     9601 cgtctaactc gcacccgaga tgcgctggtt gcggatttcc cgggtctgac tcgtgaccgt
     9661 aagtacggtc gtgcggaaat tgaaggccgt gagtttatct gtattgatac cggcgggatt
     9721 gatggcacag aagacggtgt agaaacccgc atggcggaac agtcgctgct ggcgattgaa
     9781 gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg gcctgatgcc ggcagatgaa
     9841 gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct tcctggtggt aaacaaaact
     9901 gacggtctgg atcccgatca ggcagtggtt gatttctact cgcttggttt aggtgaaatc
     9961 tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc tgctggagca tgtgctgctg
    10021 ccgtggatgg aagatctcgc accgcaagag gaagtcgacg aagacgctga atactgggcg
    10081 caatttgaag cggaagagaa cggcgaagaa gaagaggaag acgacttcga cccgcaaagt
    10141 ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag gtaagtctac actcactaac
    10201 cgtattcttg gagaagagcg cgttgttgtt tacgacatgc ctggcacgac gcgtgacagc
    10261 atctacatcc caatggaacg cgatggacgt gagtatgtgc tcattgacac cgctggcgta
    10321 cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct ccgtaatcaa aacgttgcag
    10381 gccattgaag acgccaacgt ggtgatgtta gtgattgatg cgcgcgaagg tatttccgat
    10441 caggatctct cgctgctggg ctttattctc aatagtgggc gctcacttgt cattgtggtg
    10501 aataagtggg atggcctgag ccaggaagtg aaagagcagg tgaaagaaac gctggacttc
    10561 cgtctgggct ttatcgattt tgctcgtgtg cactttatct ctgccttgca cggcagtggt
    10621 gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca gctccacccg tcgtgtgggg
    10681 acctctatgc tgacgcgcat catgacgatg gctgttgaag atcaccaacc gccgctggta
    10741 cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg gttataaccc gccgattgtg
    10801 gtgattcacg gtaatcaggt gaaagacctg cctgattcct acaagcgcta cttgatgaac
    10861 tacttccgca aatcgctgga cgtaatggga tcgccgattc gtattcagtt caaagaaggg
    10921 gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa cccagatgcg taaacgtaag
    10981 cgtctgatga agcacatcaa gaaaaataaa taattatccg taagtatttt atctgccatt
    11041 ccctctatga atgtagaggg aatggctacc ggaggaattc atatggaact gcattgtcca
    11101 aaatgtcagc atgtgcttga tcaggataac ggccatgcgc gttgcccatc ctgcggggaa
    11161 tttattgaaa tgaaagctct ttgtccagac tgccatcaac cgttgcaggt gttaaaagcc
    11221 tgcggtgcag tagattactt ctgtcagcac gggcatggct tgatatcgaa aaaacgcgtt
    11281 gaattcgtgc tggcttaatg aaccttttta cgcgattttt ttaccggctg gatgtttttt
    11341 acttcacttt ctatccagcc gtcttccaga cgtgtggtta gcatttcacc cgttttaact
    11401 tgcttcactt ttttcagtac attgccgtca gtagcagtag taacgctgta tccacgcgcc
    11461 agcgttgaca gtgggcttac ggcttcgagg tgcgttactg cattaccgaa acgttcacgc
    11521 gtggcgctaa gctgtgcgcg caggatttct gctaaacgat attccagttg ctgaatgcgc
    11581 gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat tcagccgctg tgttaatcgc
    11641 tgctgctgtt gcccggcacg cttaagctga ttttccagcg caaagctcat ccgtttttgc
    11701 aggcgttcaa gcatggtttg ctggcgtgcc agccggagct gcggatgctg ctgctgcaag
    11761 cgatgatgaa tctgcgtaaa gcgacgtgta cggttggcga gataataatc catcgccatc
    11821 tccagccgtt gacgggccga ttgcacctgg cgcagtaact cttgctgatt acggctcact
    11881 acttcagcgg cggcagacgg cgttggcgca cgcagatcgg caacaaaatc ggcaatggtc
    11941 acatccgtct catgcccgac ggcgctgacg accggaatgc ggctggcaaa aatcgcccgc
    12001 gctacgcgtt cgtcgttaaa actccataaa tcttccagcg aaccgccgcc gcgcccaacg
    12061 atcaacacgt cgcactcatt gcgctgattc gccagttcaa tggcgcgaac aatttgcccc
    12121 ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca ccggtagaga aggatcgcga
    12181 cgttttaaca catgcaaaat atcatgtagc gcagcaccgg tttttgaggt gatcacacca
    12241 acgcaatgcg caggggaggg aagtggtttt ttgtattgct gatcgaacaa acattcagcc
    12301 tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca gcccttcacc ggccggctgc
    12361 atactctcaa cgattatctg gtagtcgccg cgcggctcgt agagcgtaat attggcgcga
    12421 actaaaactt gttgcccatg ctgcggacgg aaggtgaccc ggcggttgct gttgcggaac
    12481 atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa agtaccagtg accggaagcc
    12541 ggttgtgtga aattagagat ttcgccgctg atccaaacct gtcccatctc atgctcaagc
    12601 agcagacgaa ccgtttgatt caggcgacta acggtaaaaa ttgcagggga ttgagaaggt
    12661 aacatgtgag cgagatcaaa ttctaaatca gcaggttatt cagtctatag taacccgccc
    12721 ttcggggata gcaagcattt tttgcaaaaa ggggtagatg caatcggtta cgctctgtat
    12781 aatgccgcgg caatatttat taaccactct ggtcgagata ttgcccatgc tacgtatcgc
    12841 taaagaagct ctgacgtttg acgacgttct cctcgttcct gctcattcta ccgttctgcc
    12901 gaatactgct gacctcagca cccagctgac gaaaactatt cgtctgaata tccctatgct
    12961 ttccgcagca atggataccg taacggaagc gcgcctggct attgctctgg ctcaggaagg
    13021 cggtatcggc tttatccaca aaaacatgtc cattgaacgc caggcagaag aagttcgccg
    13081 tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag actgttctgc caaccacgac
    13141 gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt gcgggctatc cggtcgttac
    13201 cgaagaaaac gaactggtgg gtattatcac cggtcgtgac gtgcgttttg ttaccgacct
    13261 gaaccagccg gttagcgttt acatgacgcc gaaagagcgt ctggtcaccg tgcgtgaagg
    13321 tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa cgcgttgaaa aagcgctggt
    13381 ggttgatgac gaattccacc tgatcggcat gatcaccgtg aaagacttcc agaaagcgga
    13441 acgtaaaccg aacgcttgta aagacgagca aggccgtctg cgtgttggtg ctgcagttgg
    13501 cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt gccgctggcg ttgacgttct
    13561 gctgatcgac tcctcccacg gtcactcaga aggcgttttg cagcgtatcc gtgaaacccg
    13621 tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg gcaacagctg caggtgcacg
    13681 cgctttggca gaagctggtt gcagcgcggt taaagtcggt atcggccctg gttctatctg
    13741 tacgactcgt atcgttactg gcgtaggtgt tccgcagatc actgccgttg ctgacgcagt
    13801 tgaagccctg gaaggcaccg gtattccggt tatcgcagat ggtggtattc gcttctccgg
    13861 cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg atggtaggtt ccatgctggc
    13921 gggtactgaa gaatctccgg gtgaaatcga actctaccag ggccgttctt ataaatctta
    13981 ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc tctgaccgtt atttccagag
    14041 cgataacgct gccgacaaac tggtgccgga aggtatcgaa ggtcgcgtag cttataaagg
    14101 tcgcctgaaa gagatcattc accagcagat gggcggcctg cgctcctgta tgggtctgac
    14161 cggctgtggt actatcgacg aactgcgtac taaagcggag tttgtacgta tcagcggtgc
    14221 gggcattcag gaaagccacg ttcacgacgt gaccattact aaagagtccc cgaactaccg
    14281 tctgggctcc tgattctctt cgcccgattt catgtcgggc gatttatatt atctgtttca
    14341 cttgcctcgg aataagcgtc aatgacggaa aacattcata agcatcgcat cctcattctg
    14401 gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg tgcgtgagct gggtgtttac
    14461 tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc gtgacttcaa tccaagcgga
    14521 attattcttt ccggcggccc ggaaagtact actgaagaaa acagtccgcg tgcgccgcag
    14581 tatgtctttg aagcaggcgt accgatattc ggcgtttgct atggcatgca gaccatggca
    14641 atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg aatttggcta cgcgcaggta
    14701 gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag atgcgctgac cgcagacggt
    14761 aaaccgctgc tcgatgtctg gatgagccac ggcgataaag ttaccgctat cccgtccgac
    14821 ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca ttatggctaa cgaagaaaaa
    14881 cgcttctatg gcgtacagtt ccacccggaa gtgactcaca cccgccaggg tatgcgcatg
    14941 ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc tgtggacgcc agcgaaaatt
    15001 atcgacgatg ctgtagctcg catccgcgag caggtaggcg acgataaagt catcctcggc
    15061 ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc tgcaccgtgc tatcggtaaa
    15121 aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc tcaacgaagc agagcaggtt
    15181 ctggatatgt ttggcgatca ctttggtctt aacattgttc acgtaccggc agaagatcgc
    15241 ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa aacgtaaaat catcgggcgc
    15301 gttttcgttg aagtattcga tgaagaagcg ctgaaactgg aagacgtgaa gtggctggcg
    15361 cagggcacca tctaccctga cgttatcgaa tctgcggctt ctgcaaccgg taaagcacac
    15421 gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag agatgaagat gggcctggtt
    15481 gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga ttggtctgga gctgggcctg
    15541 ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag gccttggcgt tcgtgttctg
    15601 ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg ctgacgccat cttcattgaa
    15661 gaactgcgta aagcggacct gtacgacaaa gtcagccagg cgttcaccgt cttcctgcca
    15721 gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg actgggttgt ctctctgcgt
    15781 gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc atctgccgta cgatttcctc
    15841 ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta tttcccgcgt ggtgtatgac
    15901 atcagcggca agccgccagc taccattgag tgggaatgat tttgatccct gcactatgaa
    15961 tgaacaaaac cctccgttac tacagagggt tttttatctt caagaatcat aggcttgaag
    16021 ttactaacat cgattaatta aaccagctgt ccgatttgtt ctcttctgct ttgcccacgc
    16081 ttttcatcag atcgcgaccg ccttcagtca tatttctgtt tgcgtcagct tcagattgca
    16141 ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc gataaattcg ttttcgcgct
    16201 taacgcgggc ttcttctttc gccagcgcca gtttttgttt ctgaatctct aagctgcgta
    16261 gctcatcttc ataactttga tcgcgttttt tgtccgcagt ggcttcggcg tccagtttat
    16321 cctgacgagc tttcttattt gccgctgccg ttgccgctct tttgttcgcg gcggcctggg
    16381 catttgcgcg acgttgcttc tcttgctgga tttccctgtt gcgctccgcg acccattcgt
    16441 catgctgcct ttgctcttca tttttacctt gctgttccgc ttctgcgaca gcagagagtt
    16501 gatcctgcaa tgatgaggct atagccggat agcttaagga ggctaagatg gcgcaaagaa
    16561 aaactttctt catgactcct cctgattatt agctcttttc aggacatttg gtatttggct
    16621 gaatacgcgt ttcgttatac gttgtggtaa taacaacggc taaacctgtc gtaaactggc
    16681 actctttacc ctcctgggtg gaggtataca ctttggtgcc ttccttatat gttaaagaaa
    16741 caccttccac taaggtttta tcattcacca cagaacccgc tgccgcgcct acagctccgc
    16801 cgccaactgc ccctgccgtc gttccggaat tgctgccaga accgacgttg tggccgataa
    16861 caccgccagc gactgcgcca ataagcgcgc cgaaggcttg tgcgttccgt ttattttggg
    16921 agttgtctac ggcaactttt gcgggaagaa tggaaataat attaacggtt ttagtttctt
    16981 gtttggtatt cagttgatcg gtttgataaa catcggcggc atgatcgtca gcatttgact
    17041 ggcatcctgc cagagtgaat gacgctaaca ttgccacagg cagaagacat tttttaaatt
    17101 tcatcactat tccttgttat acacattacg aaaaataaga catttaagat catcaaaaga
    17161 ggaaagtaaa tgtattgcgt ataaagggat agattaaatt tattaaaatt cacgcaggga
    17221 ataatttata aaaatatatc attacaacat atatattaat tttatattta tttcgcagcc
    17281 aaacaggata aacatcagtc aggacaaaga cgtaaaattt aagatgaaag aatatctgcc
    17341 gctttgataa tcccaaagag caaaagcatc cgacagcgaa gtgccgttat gttctgtgaa
    17401 agtggagaag gtaaccgcta acgttgtacc ggtgattcga cggagagtgt ggattgctga
    17461 gcttcttttt tgctctgatg gtgctgccag gcaccgacgg acgagtagat aaaacggcca
    17521 aagaagaaga taaagctgat gagcagtacg atacgggtca tgcgactgtt aaatcggtgt
    17581 cgttttcgca tactagttgc ctgactcaca aaaggttcct tgaagtatgt cccacgctgt
    17641 ggacggtact tacattaagg cacaacagga caaaatggtc aattcttcgt tatgtaaaaa
    17701 agcgtcagtg gatacatatt ttaatgttat ggaagttaat ttaattattt acaatgatga
    17761 tgtaataatg atgaattaac atgataaacg taataattca ttaatcaggg ttattttgtt
    17821 tgatatatat caattggaac tttcatatga cacttagaat caatgctctc tatagtgatg
    17881 aataatcatc attcgaagtc aggtgggatg cctgtctgaa tacacctcct tcaggatgtg
    17941 ggggattctg ctgagcatct atgaaactga atgcaactta tataaaaata cgtgataaat
    18001 ggtgggggct tccgctgttc ctgccttctt taatcttgcc cattttcgcc cacattaata
    18061 ctttcgcgca tatttcttcc ggtgaggttt ttctctttta tctgcctttg gcactgatga
    18121 tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcacctta gggatttttg
    18181 tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct aattttatta
    18241 tcattatcat tctctgttgg ggcggttaca gggtctttac tccccggcgt aacaacgttt
    18301 cacatggtga tacccgttta atttcccagc gtatattctg gcagatagtg tttcctgcaa
    18361 cgctgtttct gatacttttc cagtttgctg catttgtagg attactggcg agcagagaaa
    18421 atctggtcgg tgtcatgcct tttaacctcg ggactttaat caattatcag gccttactgg
    18481 tgggtaatct gatcggtgtc ccgctgtgct acttcatcat tcgggtagtg cgaaatccat
    18541 tttatttacg tagctattat tcgcaattaa aacagcaggt tgatgccaaa gtcaccaaaa
    18601 aagagttcgc gctctggcta ctggcattag gtgctttatt gttgctgtta tgcatgccgt
    18661 taaatgaaaa aagcacaatt tttagcacca attatacctt gtcattattg ctgcccctga
    18721 tgatgtgggg agcaatgcgc tatggttata agctgatttc gctgctctgg gcggtcgtgt
    18781 tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc tataccacgc
    18841 agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat tatatggctg
    18901 tactggcaac tcgtcagcga gcggtagtca gacgcattca gcggcttgcg tatgtggacc
    18961 cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttgcgtgat gccccctggt
    19021 ctgcgctttg ttatttacgc atccctggca tggaaatgct ggttaagaac tatggcatc
//
LOCUS       DAJTRM010000080        18793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRM010000080 DAJTRM010000000
VERSION     DAJTRM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.080"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(139..3654)
                     /locus_tag="OO322_002935"
     CDS             complement(139..3654)
                     /locus_tag="OO322_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5907737.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO322_002936"
     CDS             complement(4107..5369)
                     /locus_tag="OO322_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5907738.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO322_002937"
     tRNA            complement(5567..5642)
                     /locus_tag="OO322_002937"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO322_002938"
     CDS             complement(5748..6455)
                     /locus_tag="OO322_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5907739.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO322_002939"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO322_002939"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5907740.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO322_002940"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO322_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5907741.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO322_002941"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO322_002941"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5907742.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO322_002942"
     CDS             complement(11639..11914)
                     /locus_tag="OO322_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5907743.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO322_002943"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO322_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5907744.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO322_002944"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO322_002944"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5907745.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO322_002945"
     CDS             13874..14200
                     /locus_tag="OO322_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5907746.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO322_002946"
     CDS             14384..15103
                     /locus_tag="OO322_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5907747.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO322_002947"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO322_002947"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5907748.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO322_002948"
     CDS             16442..17404
                     /locus_tag="OO322_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5907749.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18793)
                     /locus_tag="OO322_002949"
     CDS             complement(17505..>18793)
                     /locus_tag="OO322_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5907750.1"
                     /translation="AITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAAITVS
                     QRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANM
                     MFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFS
                     LTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDAL
                     PSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTT
                     SIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMT
                     PAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQH
                     LIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4895 a         4582 c         4644 g         4672 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cga
//
LOCUS       DAJTRM010000081        18048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRM010000081 DAJTRM010000000
VERSION     DAJTRM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.081"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            246..770
                     /locus_tag="OO322_002950"
     CDS             246..770
                     /locus_tag="OO322_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5907751.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            851..1525
                     /locus_tag="OO322_002951"
     CDS             851..1525
                     /locus_tag="OO322_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5907752.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1541..4003
                     /locus_tag="OO322_002952"
     CDS             1541..4003
                     /locus_tag="OO322_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5907753.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGATVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4019..5052
                     /locus_tag="OO322_002953"
                     /pseudo
     CDS             4019..5052
                     /locus_tag="OO322_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5134..5472)
                     /gene="rcnB"
                     /locus_tag="OO322_002954"
     CDS             complement(5134..5472)
                     /gene="rcnB"
                     /locus_tag="OO322_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5907754.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5691..6521)
                     /gene="rcnA"
                     /locus_tag="OO322_002955"
                     /pseudo
     CDS             complement(5691..6521)
                     /gene="rcnA"
                     /locus_tag="OO322_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6643..6915
                     /gene="rcnR"
                     /locus_tag="OO322_002956"
     CDS             6643..6915
                     /gene="rcnR"
                     /locus_tag="OO322_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5907755.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7007..7103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7138..7926
                     /gene="thiM"
                     /locus_tag="OO322_002957"
     CDS             7138..7926
                     /gene="thiM"
                     /locus_tag="OO322_002957"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5907756.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7923..8723
                     /gene="thiD"
                     /locus_tag="OO322_002958"
     CDS             7923..8723
                     /gene="thiD"
                     /locus_tag="OO322_002958"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5907757.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8788..9609
                     /locus_tag="OO322_002959"
     CDS             8788..9609
                     /locus_tag="OO322_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5907758.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9657..10403
                     /locus_tag="OO322_002960"
     CDS             9657..10403
                     /locus_tag="OO322_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5907759.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10377..11342)
                     /locus_tag="OO322_002961"
     CDS             complement(10377..11342)
                     /locus_tag="OO322_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5907760.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11339..12342)
                     /locus_tag="OO322_002962"
                     /pseudo
     CDS             complement(11339..12342)
                     /locus_tag="OO322_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12382..13434
                     /gene="fbaB"
                     /locus_tag="OO322_002963"
     CDS             12382..13434
                     /gene="fbaB"
                     /locus_tag="OO322_002963"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5907761.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13742..14596
                     /gene="gatY"
                     /locus_tag="OO322_002964"
     CDS             13742..14596
                     /gene="gatY"
                     /locus_tag="OO322_002964"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5907762.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14625..15887
                     /gene="gatZ"
                     /locus_tag="OO322_002965"
     CDS             14625..15887
                     /gene="gatZ"
                     /locus_tag="OO322_002965"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5907763.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15897..16349
                     /gene="gatA"
                     /locus_tag="OO322_002966"
     CDS             15897..16349
                     /gene="gatA"
                     /locus_tag="OO322_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5907764.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16380..16664
                     /gene="gatB"
                     /locus_tag="OO322_002967"
     CDS             16380..16664
                     /gene="gatB"
                     /locus_tag="OO322_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5907765.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16668..18023
                     /locus_tag="OO322_002968"
     CDS             16668..18023
                     /locus_tag="OO322_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5907766.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4718 a         4108 c         4570 g         4652 t
ORIGIN      
        1 ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa tctacattaa
       61 ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat attgcattgg
      121 ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt tttgatgtga
      181 gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt attaaggaac
      241 atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt atgagcgctg
      301 gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat attgcagaat
      361 ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg actgtaccaa
      421 ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc ggtgtaacca
      481 gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc tcgttgatca
      541 caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat accgtaggtg
      601 ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat aaaacagcgg
      661 aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat aactcagtgc
      721 aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa taaataaaat
      781 tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt ttaatttaag
      841 aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc ttcctgcaca
      901 tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata aagaagttat
      961 ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga ttgatgatgg
     1021 cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc caccagtggc
     1081 aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga ataaactgcc
     1141 cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa atagtccaga
     1201 gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt tgttttaccg
     1261 gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg taaatcgcag
     1321 tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt cggatgtcaa
     1381 agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag aaagtcagag
     1441 tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca atgaccacgg
     1501 caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact
     1561 tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt
     1621 tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga
     1681 tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg
     1741 cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat caagagaagt
     1801 tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat gtttaacatt
     1861 tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc gtctcgattt
     1921 cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac cggaaaactg
     1981 ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt acagcgacta
     2041 taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt taaatttact
     2101 ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc caggggtgtg
     2161 gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca cgcttcgcgt
     2221 gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt
     2281 gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac gggtgcaggg
     2341 gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg tttatcagaa
     2401 agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg gtggagcaga
     2461 tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg tgccttatgc
     2521 agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg cgggggcgag
     2581 caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact tattgacgct
     2641 gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa cgggttggaa
     2701 cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac aagacaacgg
     2761 cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga gccaaacgtc
     2821 gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga catttaacga
     2881 tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata tctatgacat
     2941 tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca atatgagcca
     3001 gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag attactgggg
     3061 gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc ggcggataag
     3121 ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga aacgttttaa
     3181 tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc gtcggcaaat
     3241 atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata atacgggatt
     3301 atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga gtcatcaaca
     3361 tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg ttgcgacagt
     3421 gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccactg tttcaggggg
     3481 cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa cgtttgctgt
     3541 gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc gcacaacaaa
     3601 ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc acctgatgct
     3661 ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa ttgccgcccc
     3721 ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc cctggtttat
     3781 aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca atgatatcca
     3841 tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca ccaatgaaat
     3901 accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca caatcacctt
     3961 cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag gtggttctat
     4021 ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga gctatgcggc
     4081 atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt ttgcacttac
     4141 tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg tgagtacaaa
     4201 tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg ataccattaa
     4261 attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa ataatcagag
     4321 cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg caaaagtgaa
     4381 tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat tatccagtgc
     4441 ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat taggtggcaa
     4501 gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg caggtgtcta
     4561 ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac aggacgattt
     4621 attaattact ttagacagta ttcccgtttt acaattacca gccacaggta acaaagcaac
     4681 aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag gtcaacaaaa
     4741 tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa ccaacagcaa
     4801 cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag taatggttcg
     4861 ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt aaaagaaatt
     4921 gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc cagttactac
     4981 gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt aatcaacgtg
     5041 aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat aacaaggtgt
     5101 ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa tctcaccgtc
     5161 gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc cacccatata
     5221 ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact gcttaatgtt
     5281 gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat gcttaaagtc
     5341 agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg aacccgcggt
     5401 ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca acatcttatt
     5461 tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat atctttcctg
     5521 tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc gtaaaaagtg
     5581 taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg aaatagctga
     5641 tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag ttatcgcatt
     5701 atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa cagactggaa
     5761 aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc gacctgctga
     5821 acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc aatgctgaaa
     5881 ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat caacagcacg
     5941 gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa aatttgccag
     6001 ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc tcgtgcatgg
     6061 gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg gtgatgttcg
     6121 tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc cagccagttg
     6181 cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat gatcactgcg
     6241 gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact gatcaccatc
     6301 ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc cagtccgagc
     6361 atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat catcgttttt
     6421 gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg gatgaagaac
     6481 caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat tcttagtatt
     6541 aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc agtagattaa
     6601 tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca tacaatccgt
     6661 gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt ggcgctcaag
     6721 aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc tgctatccgt
     6781 ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga acacatcgtt
     6841 caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa ggtgctggat
     6901 tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct cgtttacaac
     6961 gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa acgactcggg
     7021 gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat aatgccagcg
     7081 tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga gcaaactatg
     7141 caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt tcaccaacat
     7201 tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc caataccttg
     7261 ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag tcagtttgcg
     7321 gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg cgcacaggcg
     7381 atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct tgatccagta
     7441 gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc ttttaaaccg
     7501 gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc taatggcgga
     7561 cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca aacactggca
     7621 cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac cgatggacat
     7681 cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg aactggctgt
     7741 gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct ggaaaatgtc
     7801 gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag aagcgagggg
     7861 ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca ggaggtgcag
     7921 gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt ggtgcgggga
     7981 ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca gttattactg
     8041 cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag cctgattttg
     8101 tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact aaaatcggta
     8161 tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt tatcagatcc
     8221 aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg ctttcacctt
     8281 cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata acgccaaact
     8341 tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag gaaatgctgg
     8401 aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa ggtggtcatc
     8461 tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa cggtttaccg
     8521 caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct gcggcgttgg
     8581 ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca aaaatctggc
     8641 tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt ggtccggttc
     8701 accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat tgtcagcctt
     8761 tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg aaaaaaactc
     8821 accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc gcgtcagacg
     8881 gtgaattttt tctattcgac agcagttcag attacagact acatccactt ctacggttat
     8941 cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca cggattagat
     9001 gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg cgacaacgat
     9061 atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt ggacccctat
     9121 ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc gtatcattat
     9181 ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac ggtggatttc
     9241 tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt atcggcaaaa
     9301 gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac gggaaaaaag
     9361 ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta tttcaatgaa
     9421 tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg catggcctgg
     9481 cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc ggtggatttt
     9541 aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt aaagaaacgc
     9601 cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa agacaaatgg
     9661 aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg gcggataaca
     9721 cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc ggggtgctgg
     9781 agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc gtttcgcgca
     9841 ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg cgttcgcgcg
     9901 gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg agaggttttt
     9961 ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat aagcgtgtcg
    10021 tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt gatgtctttt
    10081 tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa tcgtgggttc
    10141 cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac tatttccgca
    10201 gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg ccggatgccg
    10261 agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc aagcaagtgg
    10321 cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc agcgatctat
    10381 acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc gccccgatgc
    10441 ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag tggcagacca
    10501 gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat ggtgtctaca
    10561 acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata ccatgcgcct
    10621 tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg cttgccaagt
    10681 gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc ttgacgattg
    10741 agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg gatatcgcca
    10801 atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag ccattccact
    10861 aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag gttgccaggc
    10921 gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc gctgaatgac
    10981 ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt atcgctttcg
    11041 gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat aatctccgcc
    11101 cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg ctttaacgcc
    11161 acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag ttcgatatcg
    11221 cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc cgcgccgacg
    11281 accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg agcgccgctc
    11341 ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg ttgaaatcaa
    11401 gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta atgccatgca
    11461 acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg ccaagattgg
    11521 cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc gcaatggcgc
    11581 acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc tgttcgctgg
    11641 cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga cgtgccgcca
    11701 gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt gaaggcaatg
    11761 aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc catgcgatga
    11821 ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc agcgccacat
    11881 catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc gagacgcgca
    11941 tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta ccgtcgcgaa
    12001 tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag gcgtcgaaac
    12061 gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg ccttcacatt
    12121 ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt cggcgcggtt
    12181 aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa tccagccaaa
    12241 gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg catcccctaa
    12301 cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc cccttgaatc
    12361 ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac
    12421 aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct ccccggacat
    12481 gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg
    12541 cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt
    12601 gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac
    12661 ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc atcaacttac
    12721 ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct cgtcaaactt
    12781 aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg
    12841 gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt tggctcggaa
    12901 gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca cgagctgggc
    12961 atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga tggcgttgat
    13021 taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac cataggtgca
    13081 gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt
    13141 tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt
    13201 tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg cggtgctgca
    13261 ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc
    13321 ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa
    13381 ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc ctgatacgcc
    13441 tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat ccggcaacct
    13501 atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca cgtatgttta
    13561 caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt
    13621 tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc attatctcga
    13681 catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac aagatatgaa
    13741 aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt
    13801 tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa
    13861 cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg ctggtacaga
    13921 aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca
    13981 tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc
    14041 agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt
    14101 ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc aacttggcgg
    14161 ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc ccgcacaggc
    14221 gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg gcacggctca
    14281 cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca ttcgccagtg
    14341 ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg atattcagca
    14401 aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctt
    14461 gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc gggattattt
    14521 gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg
    14581 cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg
    14641 cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg
    14701 ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg attgaagcaa
    14761 cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg
    14821 aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt attctcggcg
    14881 gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat
    14941 ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt
    15001 caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg gaacgagctg
    15061 ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag caactgagct
    15121 atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt cagtcagtac
    15181 acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag gcctttattg
    15241 cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag ccgggtgtgg
    15301 aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggcg ctggcgcaat
    15361 ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag acccggacgg
    15421 cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc gcattaacct
    15481 ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt atcgcccccg
    15541 aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa ccgcaatact
    15601 ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt tacagcctgt
    15661 cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt gaaacgatga
    15721 tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat cttcccaaac
    15781 aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt attatgaata
    15841 aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga cggtatatga
    15901 ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt ttaacctata
    15961 tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag gcattaattg
    16021 ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc
    16081 attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag
    16141 ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt attgcgttga
    16201 ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac
    16261 agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag gaatacttca
    16321 caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta
    16381 tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg
    16441 aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc cagtgtcggg
    16501 ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact gccagggtgg
    16561 atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct ggtgtcggta
    16621 tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg ttttcagaag
    16681 tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc atcattattt
    16741 tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg catatcggga
    16801 ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt ggtccggcgg
    16861 cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg
    16921 gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg attgcgatat
    16981 tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga
    17041 atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt tcatggatga
    17101 tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc gactggtttg
    17161 cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac ggtacgtcgg
    17221 cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta
    17281 accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt ggcgaacctg
    17341 tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac gatgtcaaag
    17401 gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca cggatgatta
    17461 aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga
    17521 agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg gggcatacgg
    17581 cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct gtttgtgttc
    17641 cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc gtggcgatgg
    17701 cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc atcattatga
    17761 gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg gcggctaatg
    17821 ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt tctcccatta
    17881 cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt attatcggcg
    17941 caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt actaaacaag
    18001 agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcc
//
LOCUS       DAJTRM010000082        17603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRM010000082 DAJTRM010000000
VERSION     DAJTRM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.082"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            91..774
                     /locus_tag="OO322_002969"
     CDS             91..774
                     /locus_tag="OO322_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5907767.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            818..5480
                     /locus_tag="OO322_002970"
                     /pseudo
     CDS             818..5480
                     /locus_tag="OO322_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5848..7503
                     /gene="lldP"
                     /locus_tag="OO322_002971"
     CDS             5848..7503
                     /gene="lldP"
                     /locus_tag="OO322_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5907768.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7503..8279
                     /gene="lldR"
                     /locus_tag="OO322_002972"
     CDS             7503..8279
                     /gene="lldR"
                     /locus_tag="OO322_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5907769.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8276..9466
                     /gene="lldD"
                     /locus_tag="OO322_002973"
     CDS             8276..9466
                     /gene="lldD"
                     /locus_tag="OO322_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5907770.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9662..10135
                     /gene="trmL"
                     /locus_tag="OO322_002974"
     CDS             9662..10135
                     /gene="trmL"
                     /locus_tag="OO322_002974"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5907771.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10188..11009)
                     /gene="cysE"
                     /locus_tag="OO322_002975"
     CDS             complement(10188..11009)
                     /gene="cysE"
                     /locus_tag="OO322_002975"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5907772.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11089..12108)
                     /gene="gpsA"
                     /locus_tag="OO322_002976"
     CDS             complement(11089..12108)
                     /gene="gpsA"
                     /locus_tag="OO322_002976"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5907773.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12108..12575)
                     /gene="secB"
                     /locus_tag="OO322_002977"
     CDS             complement(12108..12575)
                     /gene="secB"
                     /locus_tag="OO322_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5907774.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12638..12889)
                     /gene="grxC"
                     /locus_tag="OO322_002978"
     CDS             complement(12638..12889)
                     /gene="grxC"
                     /locus_tag="OO322_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5907775.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13031..13462)
                     /locus_tag="OO322_002979"
     CDS             complement(13031..13462)
                     /locus_tag="OO322_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5907776.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13707..15251
                     /gene="gpmM"
                     /locus_tag="OO322_002980"
                     /gene_synonym="pgmI"
     CDS             13707..15251
                     /gene="gpmM"
                     /locus_tag="OO322_002980"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5907777.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15261..16544
                     /gene="envC"
                     /locus_tag="OO322_002981"
     CDS             15261..16544
                     /gene="envC"
                     /locus_tag="OO322_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5907778.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16548..17507
                     /locus_tag="OO322_002982"
     CDS             16548..17507
                     /locus_tag="OO322_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5907779.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17494..>17603)
                     /locus_tag="OO322_002983"
     CDS             complement(17494..>17603)
                     /locus_tag="OO322_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5907780.1"
                     /translation="VKVGYQALLWSFRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         4394 a         4582 c         4616 g         4011 t
ORIGIN      
        1 tcattaatgt aaagcaacat agctcaccaa taatacaatg gacaactcta ccggcattga
       61 gcgctggtcg gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct
      121 gtcactgttt cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca
      181 gaaagtagtt tactgaaaca acaagaagaa cataataata aaattgtgct acttgaaaaa
      241 caacaagcac agctaaagag tcaactcgaa actattcaaa aacaacaaac tggcataata
      301 aacagtacaa agactttaac tcacgtaatt aaatccgtta aagaccaaca aaatactttt
      361 atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa cggttcggtg
      421 gctttagcgg gtcgagtgtt atccattgac gccacagaga atggtagtgt tattcatatc
      481 tcactggtca acttattaag tacacctatc tcaaacattg gttttaatgc gacatggggt
      541 ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca
      601 tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa tttgaccctg
      661 aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat ggaaaatatt
      721 cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag
      781 ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat
      841 ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa
      901 gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc
      961 gtttggggca ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg
     1021 atcagctggc gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa
     1081 caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc
     1141 tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc
     1201 aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata
     1261 ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga
     1321 ctcttccaaa gcggaaggag caaacgccat tgccctgggg cgtagcagtg tagctagcgg
     1381 tactgacagc ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat
     1441 aggtgctgag accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa
     1501 agggactaat agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc
     1561 attaggaaat ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa
     1621 agctgatggc gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac
     1681 cattgcctta ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa
     1741 tagcagtgcc aacggtatta actctgtcgc gctgggcgca gattccattg cggatttaga
     1801 caataccgtc tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg
     1861 cgcgatcaag tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga
     1921 ctcggtagca aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta
     1981 cagcaccaac ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg
     2041 tacttgatga aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg
     2101 gtactagtag cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct
     2161 ccagtaagga tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca
     2221 acaccaccaa tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc
     2281 tctggaacga aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa
     2341 tcaccaacgt taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc
     2401 agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt
     2461 ccaatattgc cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg
     2521 gtgaggatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg
     2581 agaccaccag caaaataacc aacgttaaag atggcgacct gacgactggc agcaccgatg
     2641 ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata
     2701 tcgccactaa caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg
     2761 atgcgctgaa atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg
     2821 ccagcaaaat caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta
     2881 acggtagcca gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt
     2941 ctgtgaatac tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt
     3001 ataacgtcgg cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg
     3061 ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg
     3121 caagcgtgat cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa
     3181 acggctcaca actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg
     3241 aagtcaatgc agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg
     3301 ataatgtcgg tgatgccctg aatgctatcg ataccactcc tgacgacgct ctgctctggg
     3361 atgcggacgc cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca
     3421 gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg
     3481 gctcacaact ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag
     3541 tcaacgctga cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca
     3601 acgtcggtga cgccctggat gcgcttgatg ataacgccct gctgtgggat aagactgcca
     3661 atggcggtgc tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg
     3721 tcgctaatgg cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg
     3781 cgacgaatat gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg
     3841 acgcaaccta tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg
     3901 atttagcctt taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg
     3961 ctgtcgcgtc tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata
     4021 ccggtattgc gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg
     4081 acactagcgt aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc
     4141 taggtgcatt gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg
     4201 gttcagaagc ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg
     4261 ccactacgcc aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg
     4321 gtgaagactc gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta
     4381 tcggtttgaa cactctggtt ctggctgatg cgatcaacgg cattgctatc ggcagtaacg
     4441 cacgcgcaaa ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg
     4501 cacagactgg ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt
     4561 ctgtcggtag tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata
     4621 ccgatgcggt taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc
     4681 agagcattac taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg
     4741 aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg
     4801 atggcgtaga tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg
     4861 ctgccgctga caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa
     4921 tctctgtagg ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg
     4981 ccaccgatgc ggttaacgtg tcgcagctga aatcttctga agcaggcgga gtgcgctacg
     5041 acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca
     5101 gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg
     5161 cgcagttgaa gcaaagcgcg caggaaacga agcaatacac cgatcagcgg atggttgaga
     5221 tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg
     5281 caatgaccgg tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg
     5341 gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc
     5401 gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg
     5461 gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat
     5521 tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc
     5581 aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc
     5641 ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca
     5701 atattgccaa tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa
     5761 ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg
     5821 atgtgcgtgg actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc
     5881 gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg
     5941 ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt
     6001 gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat
     6061 ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat
     6121 aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc
     6181 cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga
     6241 gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt
     6301 aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc
     6361 gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt
     6421 ggccagatgg tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg
     6481 gcgattatgg acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc
     6541 ggctcgtttg ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac
     6601 attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca
     6661 gttcgcgtat tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac
     6721 accggttaca ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct
     6781 accgtaacac tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg
     6841 tatgagtggg tgatcaacat tccggtgccg tacctcaata aactggttgc ccgtatgcca
     6901 ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc
     6961 accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg
     7021 tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc
     7081 atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg
     7141 gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg
     7201 ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa
     7261 gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc
     7321 ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta
     7381 ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc
     7441 acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct
     7501 taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga
     7561 ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc
     7621 aactcggcgt atcacgtaat tcactacgcg aggcgctggc aaaactggtg agcgaaggcg
     7681 tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg
     7741 agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg
     7801 atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg
     7861 ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc
     7921 cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata
     7981 acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc
     8041 atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc
     8101 aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg
     8161 cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg
     8221 cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat
     8281 tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt
     8341 ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt
     8401 gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac
     8461 gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg
     8521 catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc
     8581 gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg
     8641 cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga
     8701 gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg
     8761 cgcacgctac cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta
     8821 cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca
     8881 tgatttaggt aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg
     8941 ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga
     9001 tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc
     9061 agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg
     9121 tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc
     9181 cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg
     9241 tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc
     9301 gggtgtagct aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac
     9361 tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga
     9421 gttgcctacg gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc
     9481 tcaccctaac cctctccctc agggagaggg gaccgttcgg cgctgtatgt actccctcac
     9541 tctgaaacga caccgcactc tttttttctc cctcgcccct ccggggaggg ccggggtgag
     9601 gggaaaaggc cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag
     9661 tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg
     9721 tctttgcgct aataccggct ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga
     9781 cgacaagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca
     9841 tcatgactat cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac
     9901 gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg
     9961 cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat
    10021 tcgcattccg atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt
    10081 ggtgtatgaa gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact
    10141 gtatgccgga tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc
    10201 ccatactcaa atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta
    10261 tcgctgtctg gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc
    10321 accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg
    10381 ccgaggattt tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca
    10441 ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca
    10501 attaccgccg tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca
    10561 atttttgctg ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag
    10621 attgccaatg cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag
    10681 gcatgaaaac ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg
    10741 gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag
    10801 gcttcttcaa ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc
    10861 gccagcatgt agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa
    10921 aaactggcca gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc
    10981 cagacaattt ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg
    11041 cccgttctgc gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtgactgct
    11101 gcgctcgtcc ttgcgtgcac gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc
    11161 gcaatataat acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc
    11221 cagttcgcgg acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg
    11281 cgcgctttgt acatccatgc cctgaccgag catcatacca aaacggcggt tacgcgactg
    11341 gttgtcggta caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg
    11401 atcggcaccc agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc
    11461 cgtacgcgca ttcgcaccaa aaccgatacc gtcagacatc cccgcgccaa tggcaatgac
    11521 gtttttcacc gcgccgccaa gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa
    11581 acttttgccg cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc
    11641 cagcgaaata gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat
    11701 aaccgccagc ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc
    11761 ggtttccgct tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg
    11821 tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg
    11881 actggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac
    11941 atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc
    12001 atggccccag aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc
    12061 gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga
    12121 tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag
    12181 ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac
    12241 tcacgagcat acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg
    12301 gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg
    12361 gtttcttcgc ccaaggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct
    12421 gccagttggg aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg
    12481 aaaacgtgcg gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc
    12541 tggaaagtca tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc
    12601 cttaaatact gcgcaatgta ataccaacgt tcacacgtta tttcagcagg ggatccagtc
    12661 caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa
    12721 aaatctgggg aaccgtggtg caaccgctgc gtttgatcat ctcttcacgc ttggcggcat
    12781 tgccatcgat cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac
    12841 gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc
    12901 tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa
    12961 gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac
    13021 gacaggtaaa ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc
    13081 cctctttcag cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct
    13141 ggcactgcat gccagaaccg tctaccacga taaccgattt gtctttgtgc ttctcaagct
    13201 caccaacatt gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc
    13261 ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca
    13321 gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag
    13381 tcacaagaac cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa
    13441 caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc
    13501 aggaaaggag tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa
    13561 ttttgcgggg cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg
    13621 tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt
    13681 tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga
    13741 ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa
    13801 ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc
    13861 tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg
    13921 gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg
    13981 ctttctttgc taatccagtg ctggctggcg cggtacataa agcgaaaaac gcaggtaaag
    14041 cagtacacat tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca
    14101 tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc
    14161 ttgacggtcg cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga
    14221 aatttgctgc gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg
    14281 accgcgataa ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg
    14341 gtgagttcca ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa
    14401 acgacgagtt cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg
    14461 aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc
    14521 gtgcattcgt gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt
    14581 tcgtgatgct gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt
    14641 ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt
    14701 ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt
    14761 tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac
    14821 cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat
    14881 acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg
    14941 aagcggcggt taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg
    15001 ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc
    15061 gcgatccggc aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt
    15121 acgttggtga taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga
    15181 ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca
    15241 tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga
    15301 aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat
    15361 tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg
    15421 atatcgccgc aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg
    15481 cacaattgaa aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc
    15541 aaaacacgct caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac
    15601 tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc
    15661 gtcagggcga gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac
    15721 agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt
    15781 tgaagcaaac gcgtgaagaa gtcaccatgc agcatgctga actggaagag aaacaaagcg
    15841 agcaacaaac gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga
    15901 acgagcgtaa aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc
    15961 taagtgaact gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg
    16021 cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga
    16081 aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt
    16141 cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga
    16201 cgctgcatcg ctatggcgag cagctacagg gtgaattacg ctggaaaggg atggttatcg
    16261 gtgcctctga aggtactgaa gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact
    16321 ggctgcaagg ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt
    16381 acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa
    16441 ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc
    16501 gccagggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc
    16561 gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca
    16621 aacttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg
    16681 cgatgccttc cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg
    16741 cgaccaaagc gcataacagc ggtcacgaag tgttgattca tctcccgatg gcaccgttga
    16801 gtaaacagcc gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc
    16861 gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg
    16921 gcagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt
    16981 acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc
    17041 aaggtactgg cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag
    17101 cggacatccg tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca
    17161 ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc
    17221 tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata
    17281 cttctacacc gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga
    17341 agctgtgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat
    17401 taagcgaaag catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct
    17461 ggggcaaaca gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt
    17521 aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa
    17581 cgcctggtaa ccgaccttca cgc
//
LOCUS       DAJTRM010000083        17375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRM010000083 DAJTRM010000000
VERSION     DAJTRM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.083"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(31..762)
                     /gene="rlmB"
                     /locus_tag="OO322_002984"
     CDS             complement(31..762)
                     /gene="rlmB"
                     /locus_tag="OO322_002984"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5907781.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(942..3383)
                     /gene="rnr"
                     /locus_tag="OO322_002985"
     CDS             complement(942..3383)
                     /gene="rnr"
                     /locus_tag="OO322_002985"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5907782.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3422..3847)
                     /gene="nsrR"
                     /locus_tag="OO322_002986"
     CDS             complement(3422..3847)
                     /gene="nsrR"
                     /locus_tag="OO322_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5907783.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4052..5350)
                     /gene="purA"
                     /locus_tag="OO322_002987"
     CDS             complement(4052..5350)
                     /gene="purA"
                     /locus_tag="OO322_002987"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5907784.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5454..5651)
                     /locus_tag="OO322_002988"
     CDS             complement(5454..5651)
                     /locus_tag="OO322_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5907785.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5733..6737)
                     /gene="hflC"
                     /locus_tag="OO322_002989"
     CDS             complement(5733..6737)
                     /gene="hflC"
                     /locus_tag="OO322_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5907786.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6740..7999)
                     /gene="hflK"
                     /locus_tag="OO322_002990"
     CDS             complement(6740..7999)
                     /gene="hflK"
                     /locus_tag="OO322_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5907787.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8085..9365)
                     /gene="hflX"
                     /locus_tag="OO322_002991"
     CDS             complement(8085..9365)
                     /gene="hflX"
                     /locus_tag="OO322_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5907788.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9441..9749)
                     /gene="hfq"
                     /locus_tag="OO322_002992"
     CDS             complement(9441..9749)
                     /gene="hfq"
                     /locus_tag="OO322_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5907789.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9835..10785)
                     /gene="miaA"
                     /locus_tag="OO322_002993"
     CDS             complement(9835..10785)
                     /gene="miaA"
                     /locus_tag="OO322_002993"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5907790.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10778..12625)
                     /gene="mutL"
                     /locus_tag="OO322_002994"
     CDS             complement(10778..12625)
                     /gene="mutL"
                     /locus_tag="OO322_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5907791.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12635..13972)
                     /gene="amiB"
                     /locus_tag="OO322_002995"
     CDS             complement(12635..13972)
                     /gene="amiB"
                     /locus_tag="OO322_002995"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5907792.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13991..14452)
                     /gene="tsaE"
                     /locus_tag="OO322_002996"
     CDS             complement(13991..14452)
                     /gene="tsaE"
                     /locus_tag="OO322_002996"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5907793.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14424..15971)
                     /gene="nnr"
                     /locus_tag="OO322_002997"
     CDS             complement(14424..15971)
                     /gene="nnr"
                     /locus_tag="OO322_002997"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5907794.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15970..17109
                     /gene="queG"
                     /locus_tag="OO322_002998"
     CDS             15970..17109
                     /gene="queG"
                     /locus_tag="OO322_002998"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5907795.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17092..17145)
                     /locus_tag="OO322_002999"
     CDS             complement(17092..17145)
                     /locus_tag="OO322_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907796.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3937 a         4955 c         4367 g         4116 t
ORIGIN      
        1 atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg cttcaaataa
       61 gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct
      121 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
      181 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
      241 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt
      301 cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt
      361 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg cgtccgcact
      421 acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca gcaggaacgg
      481 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt
      541 cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg
      601 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
      661 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
      721 cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
      781 cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt
      841 gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt
      901 ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc
      961 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt
     1021 ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag
     1081 gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc gccttttttg
     1141 cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt
     1201 tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga
     1261 acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat caggcgttgc
     1321 cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca
     1381 atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga aattacgcct
     1441 ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc agcgacatcg
     1501 cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcaacatc
     1561 tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg cccctgctct
     1621 ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc
     1681 gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttctggatcg
     1741 taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc atcaggacgg
     1801 tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt accacccggc
     1861 agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc
     1921 ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag
     1981 atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg ctgggtctgt
     2041 tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat
     2101 gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct
     2161 tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata
     2221 tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc gtagaatttg
     2281 tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct
     2341 acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat cgggataacc
     2401 tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag cggcgttggc
     2461 ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt
     2521 tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc
     2581 agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac ttcttctttc
     2641 agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc atgggtacgc
     2701 agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc gacgatttta
     2761 cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac cacaaagccc
     2821 atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag acggctatcg
     2881 tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt
     2941 tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcacccagc
     3001 ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac
     3061 aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg accaataacg
     3121 gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc
     3181 agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg
     3241 ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt
     3301 aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt
     3361 tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc
     3421 gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg
     3481 tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc
     3541 gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca
     3601 gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg cgaatgccgc
     3661 cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga
     3721 ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc
     3781 cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta
     3841 actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg atgataaacc
     3901 agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa
     3961 cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg
     4021 gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca
     4081 tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg gtcagctctt
     4141 caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct ttcacgccga
     4201 aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt
     4261 cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa gccacgcaga
     4321 gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacaggg
     4381 agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac
     4441 gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa
     4501 acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg aggataccca
     4561 gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg
     4621 tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgcg
     4681 caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt
     4741 caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt
     4801 aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt
     4861 ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac
     4921 gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg
     4981 cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg atcagcggac
     5041 atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca
     5101 tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg ctggttacat
     5161 tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga
     5221 gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca
     5281 gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt
     5341 tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca
     5401 gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca
     5461 atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa
     5521 cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc
     5581 gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt
     5641 gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg
     5701 tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc
     5761 atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag
     5821 ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat
     5881 gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat gcggccctga
     5941 cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc
     6001 gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata
     6061 cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg
     6121 acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc
     6181 gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc cggggtagta
     6241 acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga
     6301 cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc agaacgcaga
     6361 cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt
     6421 gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg
     6481 tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg
     6541 tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg ctcataaacc
     6601 aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca
     6661 ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg
     6721 ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg
     6781 cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg
     6841 ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg
     6901 ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc aggttgccac
     6961 ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc gtctcgatat
     7021 acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc
     7081 gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga
     7141 ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac gcttctgctt
     7201 cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta
     7261 cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata cccatgtcat
     7321 acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt
     7381 ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg ctgtcggtag
     7441 cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc ggattggtga
     7501 cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac
     7561 cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg
     7621 tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca
     7681 cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg
     7741 ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac
     7801 tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt
     7861 tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc
     7921 cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac
     7981 cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct
     8041 ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg
     8101 atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc
     8161 agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa
     8221 cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg
     8281 gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa
     8341 agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc
     8401 agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca
     8461 agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg
     8521 tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga
     8581 tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc accgacatct
     8641 gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag
     8701 acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc
     8761 agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt
     8821 tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg
     8881 gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg
     8941 tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc
     9001 aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg
     9061 tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca
     9121 tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat ttcaactgct
     9181 ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc
     9241 aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct
     9301 ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca
     9361 aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg
     9421 taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc
     9481 tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt gttactgtga
     9541 tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac catctggctg
     9601 accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc ttgcagctta
     9661 ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc gttcaggaac
     9721 ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact
     9781 ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac acattcagcc
     9841 tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt tttcactgtc
     9901 aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg ccaactgtct
     9961 cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt caaggtaaga
    10021 ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca aatctcctcg
    10081 ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg
    10141 ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact gatgcacctg
    10201 atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac ccgaaatgaa
    10261 aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc ttgctgccgc
    10321 aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct ctgccgcctg
    10381 ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca acccttccag
    10441 caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt
    10501 gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga
    10561 cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag cgttcggctt
    10621 cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta tcaactctac
    10681 tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg tcggccccat
    10741 caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc agggctttta
    10801 tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc acaagttgcg
    10861 gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac cactgcgcat
    10921 gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg aatacggact
    10981 gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt tggcgtaagg
    11041 gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca atacccaatt
    11101 ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa acttttagcc
    11161 gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc aattgtgcct
    11221 gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca tcgcgctcca
    11281 gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga ctgttcgccg
    11341 caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg ggcgtttgca
    11401 aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca ttcggccagg
    11461 gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct accggctcac
    11521 gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc gggatggcac
    11581 gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc tgttgtagca
    11641 cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg aaacgcactt
    11701 cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc agatacaaca
    11761 caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg atcgcatgat
    11821 tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt tccgccagtg
    11881 cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg ccgtgttgcc
    11941 attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct aagcgccgtt
    12001 ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg ttatgcgaca
    12061 ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca tcaatatggc
    12121 taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg tagaacagat
    12181 ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc acgtccatgt
    12241 cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg
    12301 tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc aggctgataa
    12361 tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc agcgccagcg
    12421 ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt ttcgccccac
    12481 cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt tccactagtt
    12541 ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt
    12601 gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg
    12661 tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc atcggatgcg
    12721 caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt
    12781 cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg accagtactg
    12841 atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga cgtttatgta
    12901 tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac cctacccgct
    12961 gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca gactgactgt
    13021 tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc tgctccagcc
    13081 agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa gcgccagtcg
    13141 cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg ttttgcttat
    13201 gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg gttaaaacgc
    13261 ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg gcgatggtga
    13321 catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg ccgtgtccgg
    13381 catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct gccggacgcg
    13441 ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta aacggattgc
    13501 gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt ttcgcaacca
    13561 cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg acagtgtaat
    13621 tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga tcgaccacca
    13681 gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc agattattgc
    13741 cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg agcgccacgg
    13801 tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc aacgttatcc
    13861 gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca cccaccggcg
    13921 tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc atcccgccac
    13981 ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag gaaaccgcac
    14041 tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca tccgggtcag
    14101 gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg ttggcaaaat
    14161 aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac aaatcaaagt
    14221 gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg gggcttttga
    14281 cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt ttacctgcgc
    14341 ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct acccgctcgc
    14401 ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc atggttttta
    14461 tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt tgccagcatc
    14521 ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc gacacagcct
    14581 gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat aataccagag
    14641 agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat aatgcctaaa
    14701 gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac gccgccataa
    14761 cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc gacggaacag
    14821 cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg acgcttatcg
    14881 ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt gcgaaaattc
    14941 tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg accaatcacc
    15001 accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc atgcaccatc
    15061 aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag tactcggact
    15121 aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc cgccgtgccg
    15181 tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt cgggcgacgc
    15241 ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt ctcctgacct
    15301 gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac atcccgcgct
    15361 tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc ggcgttgatc
    15421 actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc aaccgccaca
    15481 atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc gcggggcgct
    15541 tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc gggccagaca
    15601 atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc cagcgcggcc
    15661 tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat gccgaccgct
    15721 ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc gcacagcacc
    15781 agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc ctcgccagcg
    15841 cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg ttctccgcgg
    15901 cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt cttcattgta
    15961 tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat taaacagtgg
    16021 gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag cgagtccgag
    16081 cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg gatggcacgt
    16141 cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg tgtaatcagc
    16201 gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa aaaccccaaa
    16261 ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct gcgcaaccga
    16321 ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt tagaccgttt
    16381 gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct cggctggaca
    16441 ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt aggcgaattg
    16501 ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg caaatgcgtg
    16561 gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt cgatgctcgc
    16621 cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga gttgcgaccg
    16681 ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc gtggaatcgc
    16741 tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca cgcaccggaa
    16801 ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga aggatcggcg
    16861 attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt aggcaatgcc
    16921 ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca cccacttctc
    16981 gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc gtgcatagtc
    17041 gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg gttaccgcgt
    17101 gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa ttcaagacga
    17161 ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac ttatttatca
    17221 ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga ttacagcctg
    17281 aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc agcaataacg
    17341 catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DAJTRM010000084        17266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRM010000084 DAJTRM010000000
VERSION     DAJTRM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.084"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..452)
                     /gene="chbR"
                     /locus_tag="OO322_003000"
     CDS             complement(<1..452)
                     /gene="chbR"
                     /locus_tag="OO322_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5907797.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVT"
     gene            complement(460..810)
                     /gene="chbA"
                     /locus_tag="OO322_003001"
     CDS             complement(460..810)
                     /gene="chbA"
                     /locus_tag="OO322_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5907798.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(869..2217)
                     /gene="chbC"
                     /locus_tag="OO322_003002"
                     /pseudo
     CDS             complement(869..2217)
                     /gene="chbC"
                     /locus_tag="OO322_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2302..2622)
                     /gene="chbB"
                     /locus_tag="OO322_003003"
     CDS             complement(2302..2622)
                     /gene="chbB"
                     /locus_tag="OO322_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5907799.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2921..3259)
                     /gene="osmE"
                     /locus_tag="OO322_003004"
     CDS             complement(2921..3259)
                     /gene="osmE"
                     /locus_tag="OO322_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5907800.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3461..4288
                     /gene="nadE"
                     /locus_tag="OO322_003005"
     CDS             3461..4288
                     /gene="nadE"
                     /locus_tag="OO322_003005"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5907801.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4518..5405
                     /gene="cho"
                     /locus_tag="OO322_003006"
     CDS             4518..5405
                     /gene="cho"
                     /locus_tag="OO322_003006"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5907802.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5365..5940)
                     /gene="ves"
                     /locus_tag="OO322_003007"
     CDS             complement(5365..5940)
                     /gene="ves"
                     /locus_tag="OO322_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5907803.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6143..6628)
                     /gene="spy"
                     /locus_tag="OO322_003008"
     CDS             complement(6143..6628)
                     /gene="spy"
                     /locus_tag="OO322_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5907804.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6958..7926)
                     /gene="astE"
                     /locus_tag="OO322_003009"
     CDS             complement(6958..7926)
                     /gene="astE"
                     /locus_tag="OO322_003009"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5907805.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7919..9252)
                     /gene="astB"
                     /locus_tag="OO322_003010"
                     /pseudo
     CDS             complement(7919..9252)
                     /gene="astB"
                     /locus_tag="OO322_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9249..10727)
                     /gene="astD"
                     /locus_tag="OO322_003011"
     CDS             complement(9249..10727)
                     /gene="astD"
                     /locus_tag="OO322_003011"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5907806.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10724..11758)
                     /gene="astA"
                     /locus_tag="OO322_003012"
     CDS             complement(10724..11758)
                     /gene="astA"
                     /locus_tag="OO322_003012"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5907807.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11755..12975)
                     /gene="astC"
                     /locus_tag="OO322_003013"
                     /pseudo
     CDS             complement(11755..12975)
                     /gene="astC"
                     /locus_tag="OO322_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13421..14227
                     /gene="xthA"
                     /locus_tag="OO322_003014"
     CDS             13421..14227
                     /gene="xthA"
                     /locus_tag="OO322_003014"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5907808.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14394..15104
                     /locus_tag="OO322_003015"
     CDS             14394..15104
                     /locus_tag="OO322_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5907809.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLHFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15109..15786
                     /gene="ydjY"
                     /locus_tag="OO322_003016"
     CDS             15109..15786
                     /gene="ydjY"
                     /locus_tag="OO322_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5907810.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15801..16508
                     /locus_tag="OO322_003017"
     CDS             15801..16508
                     /locus_tag="OO322_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5907811.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16508..17056
                     /locus_tag="OO322_003018"
     CDS             16508..17056
                     /locus_tag="OO322_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5907812.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17066..>17266
                     /locus_tag="OO322_003019"
     CDS             17066..>17266
                     /locus_tag="OO322_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5907813.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKI"
BASE COUNT         4272 a         4591 c         4223 g         4180 t
ORIGIN      
        1 gtaaccatct caacaaactc ttccagccct gtttcgcgga aatttaatga agaaatcact
       61 gtttccacat aggtgagaaa agcgttattg gtccggtata cctgcgaagc aacaaagcaa
      121 taaggcaaca acggcaggta atgctgctca aaaaagcgtt tactgatccc aacgttcaat
      181 atgcgcgtgg caccaaactc ataaaaactt tgatggtgcg aacctaacgg aataaaaaca
      241 aaatcgcccc gttccagtaa cacgcgctta ccgttaatct cctggaaata acgcccggtt
      301 aataccagag taaattcata atagtcgtgc tgatgcagtc cgctgatact ctcagttttg
      361 ttatagataa acacatggaa gtttttgcca ttaaacaact gctgttctcg ggcagtggca
      421 atttccggcg cgttaatcac tggctgcatc atcgactcct tatgccttca gtttttcatg
      481 aagctcaatt aattcagtaa tcagttcacg cgcaagcatg gacgtcatta aatgatcctg
      541 agcgtggacc agcaccagac tcactttcat cttaccttcg cccgcatcgc cttcaatcag
      601 tttcgtctgt accagatgcg cttcattcaa tgccatccgt gactgatcca tcatggcttt
      661 cgctgcggca aaatcgccct gcttcgcctg tttcaatgcg gcatacgcca ggctacgcgc
      721 ttgtccagag ttgatgataa gccccatcac cacttcttcc agctcttcag cttctgtttg
      781 cgtatcagga atattatcga gatccatcat acatcgttcc tctctcttct taccggcaag
      841 attacccgta ccggcatcga ttaaaatttc aggggttagc gatatcttct tcgctctctt
      901 ctttatcaat cgcattttgt gctttgttag ccaccacaac aaagggcaga taaattaacg
      961 ttgcgatgcc aaggttgaag agtgcgacca gcaatgcggc gacgctaccg ttggtgttaa
     1021 agaaggctcc cagaccggtt ggcatggtcc acggtgcaat attggtcacc ggaggaatga
     1081 tgcccatgta gtacgctgcg agggtgattg ccgccagaat cggttgtacc agtacaaacg
     1141 ggataaacat caccgggttc atgataattg gcagaccaaa cagaatcggt tcgttaatct
     1201 ggaagatgcc ggacggcagc gccagttttg ccacctgacg ataatcagca cggcgagagg
     1261 cgataaagat agccaggatc aggcctaaag tcgcaccact gcccccaagg aaaataaagg
     1321 agtccagcat cggcttggcc cagatatgga aggtcttacc ggctgccagc gccgcttcga
     1381 cggaaccata ttgctgatag gtcgcgatat tttccagcgc ccacggcgtc ataatgccgt
     1441 tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa gaaccagagc agtggaacaa
     1501 agatcacata ggcccagccc accacgctac ccaacgatgc cagtggggtt gagatggtat
     1561 ccataatgat ctgatggaag ttggtgcccc aggtattcaa cgcccaggca ataatcccca
     1621 tcacggaaag aataataaag ccgggaatta atgccgagaa agaacgcgat actgaagcag
     1681 gtacgctgtc gggcagttta atgacccaat tgcggcggac aataaaggta aacatttctg
     1741 ccaccaccag gccaataata atcccggaga tgatattcgc cccacctaac cagtttgcgc
     1801 caaccgcata ggcctcaccg acactatatg gggtgacggt cataaatgct gcaacggata
     1861 acaacccagc cgccagcgca tcgactttac gctcttctgc cagcgccatg ccaataaaga
     1921 acggtgccat taaagacatt attcctaatg ttccgttata tacgttgccg ccaatacctt
     1981 tcagaccatt aagtgtttca atggttgagg cgtcgaggcg aatacctaag gaataaaaaa
     2041 cgacccctcc ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag
     2101 ggttaacggc attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc
     2161 tattttaact gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga
     2221 aaaataccct taaaaaccgc aatttaaata ttacggtatt gatttatgaa ataactcttt
     2281 gacgggaaaa tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc
     2341 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg
     2401 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
     2461 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
     2521 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagagataga
     2581 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
     2641 gattatctgt cagccagaca ctccgcaagc cttgacctgc ttccatgctc tgggtaactt
     2701 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
     2761 agtatttctt tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca
     2821 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat
     2881 ggccggtttc cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat
     2941 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
     3001 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
     3061 taccgcgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
     3121 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg
     3181 tacgatcata agctgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
     3241 ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga
     3301 atacattggg cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg
     3361 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta
     3421 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     3481 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt
     3541 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     3601 cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct
     3661 gcgccaggaa actcgcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     3721 tcaggctgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     3781 aaccgttaat atcaagggcg cggtattggc tagcgagcag gcattgcggg aagcaggcat
     3841 tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     3901 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     3961 taccggattc ttcactaaat atggtgacgg cggtacggat attaatccgc tgtatcgtct
     4021 caacaaacgc cagggtaaac agttactgac ggcattaggt tgcccggaac acctttataa
     4081 gaaagcccca actgccgatc ttgaagatga tcgcccttct ctgccagatg aagtggcact
     4141 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacctac ctgaacaggt
     4201 cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa
     4261 cgttttcgat gatttctgga aaaagtaata atttgcacct attggattgt gcgaaaaaga
     4321 gtaatttgtt cacgccggat gcggcgtgaa cgccttattc tacctacaaa actatgcaaa
     4381 ttcaatacat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc
     4441 tttgtcatca acccgcctct tttttaatcg cctcccgcct gttacactgg atagataacc
     4501 agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
     4561 cggcaattta tgaatatccc gaacatttac attcattcct taatgactta cccacccgac
     4621 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg
     4681 ttaatatccg cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac
     4741 ggcaatcccg gcgaattagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg
     4801 aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc
     4861 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
     4921 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
     4981 cattgcagag catcgctgat gaacaaaaac tttgttatgg cctgttggga ctggaaccgt
     5041 taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
     5101 gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcgag
     5161 tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc
     5221 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
     5281 caacgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
     5341 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
     5401 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
     5461 atcgaaccag cacacgccct ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc
     5521 gttaatcaca aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat
     5581 tctgactttc gccttacaga catccaggcg cgtcataata ttgaaatcca tcgacatctg
     5641 ccctgccgtc agtttcgctt ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg
     5701 ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa
     5761 cgtcactatc ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc
     5821 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
     5881 ttcaccggca gcgtttcgcc acagattcac cgacatttta cgcatatcaa agtattccat
     5941 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
     6001 caactcgggt gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga
     6061 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
     6121 cttaagtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg
     6181 gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca
     6241 ggatgttgta aattttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt
     6301 cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga
     6361 tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt catctggtca cgctggcctt
     6421 tcatgatttc gtggatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca
     6481 tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg
     6541 ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg
     6601 caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac
     6661 ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt
     6721 aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga
     6781 aaaaaagaaa atttgatgga gagtgatgag agaatattac aacacgatga ttttgcagag
     6841 attatgaaga actataccgg atgactggtg ataaataaag caaataacca ggattaatct
     6901 gtattaattt ataagaaagc aacttaatac ccgcagaatg atttctgcgg gtaagtatta
     6961 gcttattttt tcgagcatta atcccgcgcg taatcccaac gctaccaacg gattagggaa
     7021 taacacatac tctacatcat gggttacggt aaaacgttcc tctccgtcct gcgccagcaa
     7081 tgttcctttc tcaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga
     7141 cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggtg ttctcacgat
     7201 accgacactc tcaccagata gcagcgcagc aattgcgctg gcagttactg caaactggcg
     7261 aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc
     7321 aaaatgtctg acgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagcgc
     7381 ctccagcccc gccgcaccca gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg
     7441 cggtaataca ccgaactgcg gatgcaagga gccacgaatt gcggtatgta gatcaaggtg
     7501 ccaacgcaca gattctttgc cctggtcata aaaatcttcc aggcactgtt ccagttcgcg
     7561 cgcccgacag gtttctccgc tttcagcaaa tagctgccaa cgaccgccaa acattcggtt
     7621 catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac
     7681 cagcaaccgc caacgtaacg ggatctcgcc gtgagatatc gcgccaagca acgcgtccag
     7741 catttccaca ggtgccgtct cattaccgtg gatccccgca gaaatcacca gcgcgccttg
     7801 cggtggcgtt aatggcgtca gttccagcac accatcgccc agccagcgcc agcgaacgcc
     7861 gttgatttct cgctcggtga taaccggttt tttacccgtt aaggtcagag caagaaaatt
     7921 atccattgcc ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc
     7981 tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag
     8041 cagtaagacg atcgcggtag taacgatcca cccagtcatt gagcgcatta aacaaggtat
     8101 cgttcatcat caccgccgga ttcaccgccc ggcgttcttc ttctgtcaat accacccgca
     8161 accgcaggca cgccgggccg ccgccattcg ccatgctttc acgtaagtca aagactttta
     8221 gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt
     8281 gttcccgaca ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt
     8341 ggctgttaaa cagataggta gacaccgcat ccgacacgga aacctgagtt gccggaactt
     8401 ctatcgccat aaaaccgttc acccgcgtgc cagtaactgt gactggcgag cgaacgcctg
     8461 ttggtggcaa aacagcacct ggcggttact cacggcaatc acgtcattat gaaaaacgct
     8521 ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgttggggat tcacctgatt
     8581 aagccttgcc accgcctcgc tggcttcgcg agtctgtcgc gccggataac gggaaggccg
     8641 ggtatcattg ccttcttctc gcccgtagac aaaaagtagc atacccggtt caccgtaatg
     8701 accgccgaga cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta cctgcggcaa
     8761 cgccgaatgg acgctaaatt tctcttcgtc cttaaaaatc gcttttaaca gcgattcagt
     8821 gacgggcgct tccagcgaac ggtgaaattt attgttcagg ttggcaacgg tgagatgcac
     8881 tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta cccacattgg
     8941 cgaagcggaa ctgacgctgg aaagccagtg cggtgcctgg cgtgcaactt tttccagtac
     9001 ctgctcatcg ctaccactga atcccaactg acgcagcacc ggaataaacg gacgctcgtg
     9061 cggcggaatc acggcctggg ggaatcccgc atcggcaagg gctttcattt tcagtaagcc
     9121 ctgttttgcc gccagtcgcg ggttagacac ctgaaaacgg tgacgggtag aggcttcatt
     9181 accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga aattaacttc
     9241 ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc cggggttaag cgtggcgggc
     9301 aatgttaacg agtccgactc caggctcgcc ataggccatg cgcagtaatc tgcggcatac
     9361 caggcgctgg ggcgatggtt accggaagca ccaatgccgc cgaatggggc ggtactggca
     9421 gcaccagtaa gcggtttgtt ccagttaaca atccccgccc gcgcctccag caacagttga
     9481 tcgaattttt cccgctcggg ggaaaccaga ccgcaaaaga gtccgaagcg agtgttgttc
     9541 gccatccgaa tcgcctcatc aaaagtatca taacgccaga cgcgcaaaaa cggcccgaac
     9601 acctcttcat ccggcacacc gccaacgccc gtcatttcaa tgatccctgg tgtcagcaac
     9661 gatgtccctg cttgtaataa gcgcggcgca agcagggttc gtccgcccat cgcttccagt
     9721 tgctgccagg cagtaaccac ctgctgtgcg gcctgttcag aaatcagccc gccaataaac
     9781 ggctgcggtt cgtcatccca gttgcccggc gttaatcgct ggctgacggc aaccagacta
     9841 gcaagaaacg catcgccctg cgctccgctt ttgagcaata aacggcgggc gcaggtgcag
     9901 cgttgcccgg ctgtgacaaa cgccgactga atggtcagat ggacagccgc gtcgatatcc
     9961 gccacctcat cgataattag cggattatta ccgcccatct caagggcgag aattttctcc
    10021 ggctgaccgg agagctggcg atgcagctgg tagccggtat tggcgctacc ggtaaacagc
    10081 aaaccgtcga gatcctccag cgcactcagc gcctgacccg tttcacgccc gccctgcacc
    10141 aggttcagca cgcccggcgg caagccagcc tgctgccata aacgcattac cgcttcgcca
    10201 ctccacggtg tcagttcgct gggtttaaag ataatggtgt tacctgccag caatgccgga
    10261 acgatatgtc cgttcggcaa atgaccaggg aaattatacg gcccaaacac cgccagcacg
    10321 ccgtgcgggc gatgtcgcag gctcgccgca ccgtccggca tttcactacg ctgctcgccg
    10381 gtacgaacgt gatacgcctt aattgatatc gcgattttat tgatcatcgc cgtcacttcg
    10441 gttgccgctt cccagcgcgg cttacccgtt tctctggcaa taatcgcggt taattcggct
    10501 ttattgcttt ccagcaatcc ggcaaagcgt tcgacaacga cctgacgttc agccaatgag
    10561 agccgcgccc agcgcggaaa cgccgcacgg gctgcccgac aagcctgttc gacctgagcg
    10621 gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg cttcacacgc
    10681 gatgcgccct ggcccgttat ccagtcaccg ttaatccata aagtcatgct gttttctcct
    10741 ctgcgcacag gcgcaccaga cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt
    10801 gtgcggcggt taaaatcaaa cgctcggttg ccggatcggt acgcgccagc accacgcgga
    10861 aatggtgata attttcattg gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc
    10921 cttctgccac ttccaccagc cgacttttac ggatggcgcg cacgcggtcg atgtcacact
    10981 caagcgttgg cccgccgtcg aagatgtcga tatagttgcg gtaacgaaaa ccttctttct
    11041 ccagcaccgc gcgggcaggc gcggtttgcg gatgtacctg accgatgacg tcctgggctt
    11101 cctgggataa aaagtgggta tagatcggat gtttcggcat cagttctgca ataaatgcct
    11161 tttgcccggt gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac
    11221 cgaggctttg ccagaacggt gaatagccgt gttcgtcaat caccccgcgc atttcagcaa
    11281 ccactttgtc attaaacttg tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca
    11341 gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc
    11401 tgctgccggt gtgatcgtta ctaagaaaca gcgtcggcaa tgcgttatag acattcagct
    11461 cttttgaggc gtgaaccaac gtgccgacgc gatagttgta ccagggatcg ttcagcccaa
    11521 ccgccacctc aatagcgcaa atccccgcca cggtgcctgt ctcgctatct tccagcacga
    11581 acacatagcc ctgctcgctt ttgggcagtt caccttgcca ggttttgatt gccctttcga
    11641 tacgcgccga aagcgtggct tcattggcgg gaagcgacgt caggccgccg cccgttttgc
    11701 tggcaagctg catcagcgcc gagacatctg agcgctcaac gggacggatg accatcatga
    11761 tgaacctcgg ctaacaaagt gttcgcaagc agctgcaaag cgatccagtc cggtcgtcac
    11821 ctcttcttcg ctgacattga gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag
    11881 taccatcacg cctgctttcg ccgcttccag agagatctgt ttcgcttgcc cggcgtaatc
    11941 ggcattcagt acacagccaa tcaacaaacc taagccgcga acttcactga acaaaccata
    12001 gcggtgatta atagtattaa gacgctcaac aaaccagtcg tgacgctgtt taacgccatt
    12061 aagcatctct ggtgtgttga tgagctccag cactttgcct gctaccgccg aggccagcgg
    12121 attaccgcca taggtggtgc catgagtgcc aacggtcatc acgcgggcgc actcttcggt
    12181 tgccaacaac gcaccgaccg ggaaaccgcc gcccagcgct ttggcggtgg ttaacagatc
    12241 gggcgtcacg ccgtagtgca tataggcata cagctcacca gtgcgcccga cgccggtttg
    12301 tacctcatca aaaatcagca aggcattgtg gcggtcacac aattcacgca gaccttgtaa
    12361 aaacgcgttg ctggctggca ccacaccgcc ttcgccctag atgggctcga caatcaccgc
    12421 acaggtggcg tcgtcaatca gcgcgctggc agagttaata tcgttatatg cagcatgacg
    12481 aatatccgcc ggcagtggcg caaaatcctg tgaataggct ggctgcccac ccgcactgac
    12541 agtaaacagc gtgcgaccat gaaacgcatt tttgaacgcc acgatgccac tcttatggct
    12601 gccgtagcgg tcgtgagcga atttacgcgc cagttttagc gccgcttcgt tggcttccgc
    12661 accggagtta caaaagaaga cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag
    12721 tcgcagtacc ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc
    12781 gttcagcgct tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg caatgccacc
    12841 cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt cgccacgtac
    12901 cggtataaag ggtgccggag cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt
    12961 aattggctga gacatagcga cctctacagt aaataattcg ttatttgtat gttaacaata
    13021 agtaatgttt gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg ataaaagtgc
    13081 ataaacggcg gaggctaact ggaaatcaag gagttataac caaaccatat gcatttaaag
    13141 tgcatataaa gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa aacgcaatgt
    13201 tatgcagaag taaaatataa ttctagaatt gtgatcattg acgaaattta ctggaaatta
    13261 ctgcgccatt ctgacgcagc gcgcaccaaa agcgggcatt ttttgcgcca tcgttgacat
    13321 cattaacaac catcgatcaa atcacttaac aacaggcggt aagcaacgcg aaattctgct
    13381 accatccacg cactctttat ctgaataaat ggcagcgact atgaaatttg tctcttttaa
    13441 tatcaacggc ctgcgcgcca gacctcacca gcttgaagcc atcgtcgaaa agcaccaacc
    13501 ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat atgtttccgc tcgaagaggt
    13561 ggcgaagctc ggctacaacg tgttttatca cgggcagaaa ggccattatg gcgtggcgct
    13621 gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg acgaagaggc
    13681 gcagcggcgg attattatgg cggaaatccc ctcactgctg ggtaatgtca ccgtgatcaa
    13741 cggttacttc ccgcagggtg aaagccgcga ccatccgata aaattcccgg caaaagcgca
    13801 gttttatcag aatctgcaaa actacctgga aaccgaactc aaacgtgata atccggtact
    13861 gattatgggc gatatgaata tcagccctac agatctggat atcggcattg gcgaagaaaa
    13921 ccgtaagcgc tggctgcgta ccggtaaatg ctctttcctg ccggaagagc gcgaatggat
    13981 ggacaggctg atgagctggg gggtggtcga taccttccgc catgctaatc cgcaaacagc
    14041 agatcgtttc tcatggtttg attaccgctc aaaaggtttt gacgataacc gtggtctgcg
    14101 catcgacctg ctgctcgcca gccaaccgct ggcagaatgt tgcgtagaaa ccggcatcga
    14161 ctatgaaatt cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg cgaccttccg
    14221 ccgctaattt agcagctctc ctggctcaaa ctgggtcagg agaattaacc ttgagaaaaa
    14281 tcaacaaact gtcagtaatg atttgttgcc tgccgtcctt tgttataccg tcactgcgtt
    14341 tttagttgtc tgaccacttc tctattatca agtttgatat aggaaactcc acgatgaacg
    14401 ctgagcgtaa ctttctgttt gcctgtctta tttttgcgct ggtcatttac gctatccacg
    14461 ctttcggttt attcgatctg ctcaccgatt taccccactt acagacactc attcgccaga
    14521 gcggattgtt cggttatagc ctctatattc tgttattcat caatgccccc ctctttctgt
    14581 taccaggaag catactggtg atcgctggag gaatagtttt tggcccgctc ttagggacac
    14641 aactctcatt aattgccgca acgctggcct cctcgtgctc attcctgctg gcgcgctggc
    14701 tgggacgtga tttactgctg aaatacgtgg gccatagcca taccttccag gccattgaaa
    14761 aaggcattgc gcgtaacggt attgattttc ttattctgac ccgcttaatc ccgttgtttc
    14821 cttacaatat tcaaaattac gcttacggat tcaccaccat cgccttctgg ccttataccc
    14881 ttatttcggc actcacgacc ctgcccggta ttgttattta taccgtgatg gcaagcgatc
    14941 tcgccaatga aggcattacg ctgcacttta ttttacaact ctgtctggcg ggcctggcgc
    15001 tgtttattct catccagctc gcaaaactct acgcccgaca caaacatgtg gatctgtctg
    15061 cttcgcgccg cagcccactt actcacccaa aaaatgaagg atagaacgat gtcgcaacat
    15121 tattcagtgt catggaaaaa aggactggct gcactctgct tactggctgt tgccgggctt
    15181 agcggctgcg atcaaaaaga gaatgcggcg gcaaaagtgg aatacgacgg actttcgaac
    15241 agccaaccgc tgcgcgtcga tgccaataac catacggtaa ccatgctggt gcaaattaat
    15301 ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt ttaaagatgg ctccaacgga
    15361 cataaatcgc tgtttatggc ttatgcgccc ccgaaagcat tttatgaagc cctgaaagag
    15421 gcaggtggta cgccgggcga aaacatgacg atggataata aagaaacgac tcatgtcaca
    15481 ggcagcaaac tggatatttc ggtcaactgg caaggggcgg caaaagcgta ttccttcgat
    15541 gaagtgattg ttgacagtaa tggcaagaaa ctggacatgc gctttggcgg taatttaacg
    15601 gcagcagaag agaagaaaac gggttgcctg gtgtgtctgg atagctgccc ggtcggcatc
    15661 gtcagcaatg caacatacac ttatggtgcg gtggaaaaac acggcgaagt gaaattcaaa
    15721 ggcaatgcct cagttctccc ggcggataac acgctggcaa cggttacctt taaaatcacc
    15781 gaataaagcc aagataaagg atgatgaaga tacaatcgcg taaaatctgg tactaccgta
    15841 taaccctcat catcctgttg ttcgctatgc tgctcgcatg ggcgctgctt cccggcgtcc
    15901 atgagtttat caatcgcagc gttgcggcgt ttgccgccgt ggaccaacag ggtatagaac
    15961 gctttattca gtcttacggc gcactggcgg cagttgtctc gttcttgttg atgattttac
    16021 aggccattgc tgcaccgcta cctgcgtttt tgatcacctt tgccaatgcg tcgctgtttg
    16081 gcgcgttctg gggcggcttg ctgtcgtgga ccagttcgat ggccggcgcg gcgctgtgct
    16141 tttttatcgc cagagtgatg ggccgcgaag tggtggaaaa attaaccggc aaaaccgtgc
    16201 ttgacagtat ggacggcttt ttcactcgct acggcaaaca caccatcctg gtctgtcggt
    16261 tattgccttt tgtccctttc gatccaatca gctatgctgc cggtctgact tcaatacgtt
    16321 ttcgctcgtt ttttatcgcc accgggcttg gtcaattacc ggcgactatt gtttattcct
    16381 gggcaggcag catgttaaca ggcggtactt tctggtttgt caccgaactg tttattctgt
    16441 ttgccctgac cgtggtgatt tttatggcga agaaaatatg gcttgagcgc cagaagagga
    16501 atgactgatg ggtttaccgc cgcttagcaa aattcctttc attttacgtc cgcaggcgtg
    16561 gctgcatcgt cgccattacg gcgaggtgct aagcccgatt cgctggtggg ggcggatccc
    16621 gtttatcttt tatttggtgt caatgtttgt tgggtggctg gagcgcaaac gctcaccgct
    16681 cgatccggta gtgcgatcgc ttgtcagcgc gcgcattgcg caaatgtgcc tgtgtgagtt
    16741 ttgcgtggat atcaccagta tgaaagtcgc cgagcgtacc ggcagcaccg ataaactgct
    16801 ggcagtggct gactggcggc aaagcccgct ctttagcgat gaagaacggc tggcgctgga
    16861 gtacgccgaa gccgcaagcg tcacaccgcc aacagtcgat gatgccctgc gtacccgact
    16921 ggctgcgcat tttgacgctc aggcgctcac cgaactgacg gcattgatcg gcctgcaaaa
    16981 cctgtcggct cgttttaatt ctgccatgga cattcccgct caggggctgt gccgtattcc
    17041 ggaaaaacgt tcttaaggag ggaagatgcg ccattgtggg tggttgctgg gattgttatc
    17101 gctgttttct ttggcaacac atgccagtga ctggcaggaa attaaaaatg aggccaaagg
    17161 gcaaaccgtc tggtttaacg cctggggcgg cgataccgca attaaccgct atctcgactg
    17221 ggttagcggc gagatgaaaa cccattacgc tataaacctg aagatt
//
LOCUS       DAJTRM010000085        16932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRM010000085 DAJTRM010000000
VERSION     DAJTRM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.085"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            354..920
                     /gene="mntP"
                     /locus_tag="OO322_003020"
     CDS             354..920
                     /gene="mntP"
                     /locus_tag="OO322_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5907814.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(917..1726)
                     /gene="rlmA"
                     /locus_tag="OO322_003021"
     CDS             complement(917..1726)
                     /gene="rlmA"
                     /locus_tag="OO322_003021"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5907815.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1892..2101)
                     /gene="cspE"
                     /locus_tag="OO322_003022"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1892..2101)
                     /gene="cspE"
                     /locus_tag="OO322_003022"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5907816.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2114..2257)
                     /locus_tag="OO322_003023"
     CDS             complement(2114..2257)
                     /locus_tag="OO322_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5907817.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2928..3215)
                     /locus_tag="OO322_003024"
     CDS             complement(2928..3215)
                     /locus_tag="OO322_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5907818.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3290..3433)
                     /gene="mgrB"
                     /locus_tag="OO322_003025"
     CDS             complement(3290..3433)
                     /gene="mgrB"
                     /locus_tag="OO322_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5907819.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3592..3831
                     /locus_tag="OO322_003026"
     CDS             3592..3831
                     /locus_tag="OO322_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907820.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3974..4765)
                     /gene="kdgR"
                     /locus_tag="OO322_003027"
     CDS             complement(3974..4765)
                     /gene="kdgR"
                     /locus_tag="OO322_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5907821.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4942..6315
                     /locus_tag="OO322_003028"
     CDS             4942..6315
                     /locus_tag="OO322_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907822.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6361..7242)
                     /gene="htpX"
                     /locus_tag="OO322_003029"
     CDS             complement(6361..7242)
                     /gene="htpX"
                     /locus_tag="OO322_003029"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5907823.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7434..9482)
                     /gene="prc"
                     /locus_tag="OO322_003030"
     CDS             complement(7434..9482)
                     /gene="prc"
                     /locus_tag="OO322_003030"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5907824.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9502..10200)
                     /gene="proQ"
                     /locus_tag="OO322_003031"
     CDS             complement(9502..10200)
                     /gene="proQ"
                     /locus_tag="OO322_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5907825.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10297..10794)
                     /gene="msrC"
                     /locus_tag="OO322_003032"
     CDS             complement(10297..10794)
                     /gene="msrC"
                     /locus_tag="OO322_003032"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5907826.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10924..12207
                     /gene="yebS"
                     /locus_tag="OO322_003033"
     CDS             10924..12207
                     /gene="yebS"
                     /locus_tag="OO322_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5907827.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12176..14796
                     /gene="yebT"
                     /locus_tag="OO322_003034"
                     /pseudo
     CDS             12176..14796
                     /gene="yebT"
                     /locus_tag="OO322_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14876..16315
                     /gene="rsmF"
                     /locus_tag="OO322_003035"
                     /pseudo
     CDS             14876..16315
                     /gene="rsmF"
                     /locus_tag="OO322_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16433..16669
                     /locus_tag="OO322_003036"
     CDS             16433..16669
                     /locus_tag="OO322_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5907828.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16774..>16932
                     /locus_tag="OO322_003037"
     CDS             16774..>16932
                     /locus_tag="OO322_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5907829.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGF"
BASE COUNT         4090 a         4274 c         4099 g         4469 t
ORIGIN      
        1 ttatttgtta tttattttaa cgtttcattg atatataaac ataaatgaaa atcgttatca
       61 ataaagcaat gaaataatat attccaacag ttgttttata ttctcaaaat atgttaaggt
      121 tgcgccctca tttggggagt agccgatttc cagattccgg aaatgtacgt gtcaacatac
      181 tcgttgcaaa acgtggcacg tacggactga agactttcag tcaggcgaga ccatatgcac
      241 atcaatcgct atgcctgcat gaacgcgaaa acccatcgcg gcttgttgtt catgtatgca
      301 tgtttgctgg gggcgatgat gtgttttatg gataccccgg tcaggacgtt gtcatgaata
      361 tcactgctac tgttcttctt gcgtttggta tgtcgatgga tgcatttgct gcatcaatcg
      421 gtaaaggtgc ccccctccat aaaccgaaat tttctgaagc attgcgaacc ggccttattt
      481 ttggtgccgt cgaaaccctg acgccgctga tcggctgggg aatgggcatg ttagccagcc
      541 ggtttgtcct tgaatggaat cactggattg cgtttgtgct gctgatattc ctcggcgggc
      601 gaatgattat tgagggtttt cgtggcgcag atgatgaaga tgaagagccg cgccgtcggc
      661 acggtttctg gctactggta accaccgcga ttgccaccag cctggatgcc atggctgtgg
      721 gtgttggtct tgctttcctt caggtcaaca ttatcgcgac cgcattggcc attggttgtg
      781 caaccttgat tatgtcgaca ttagggatga tggttggtcg ctttatcggc tcaattattg
      841 ggaaaaaagc ggaaattctc ggcgggctgg tgctgatcgg catcggcgtc cagatcctct
      901 ggacgcactt ccacggttaa taagaacgct gccagaggtg aatattaaag tccgtctggc
      961 agtcgaacac ttcttttgct gccagtgttt gccagacttc tggcttcgca cgccaggcaa
     1021 acggcgtcat ctgcaataat gcgacggctt catcaccgcg aagacgcatc ggataacaca
     1081 actccgcact ctgctgtaat gtaaaacctt ccagttgttc tgcatgaggt gcatgaagat
     1141 gtacttcatt gtaaatcagc cccttcagct ccatcaaatg tcgcggtccc ggcgtggcag
     1201 taatgaccca gccgccgggc ttcactactc gtgctaattc ttctgcttta cacggcgcgt
     1261 aaatacgtat tatggcgtcc atactggtat cggaaaacgg caaacggtgg ctggaagcga
     1321 cacaaagagt gacctgcgga tagcgtttcg ccgccgcttt tatcgctacc ttcgagacat
     1381 ccaggccaaa cgtggtgatt tcgggcaacg catcggcaaa tgcgtgtgtg taataccctt
     1441 caccacagcc aatatccagc accgccgtgg ccttttcatc aagccgttcc cgcagttggg
     1501 cgacaattgc atcacgcagc ggctgataat gtccggcatc taagaatgcg cggcgtgctt
     1561 gcatcatttc cgcgctgtcg cccggatcac gagaccgttt atgctgaacg ggcagcagat
     1621 tgacataccc ttctttcgcc atatcaaact gatgtcgttg gggacagata tagctgtttt
     1681 tttcacgcga aagaggctga tggcaaagtg gacaggaaaa cgacatgaca actccggcag
     1741 gatactaaag gccgcaagtg taacgcgaat cacgcggcgg gcaaatatcc gaacgccagg
     1801 agcgaagata aagcatagta aaaagcctcg cattcgacga ggctttatag cgagattgaa
     1861 gcgtattcac acttcagatc agtggattcg atcagatagc tgttacgtta acagctgccg
     1921 gacctttctg gccgtcctga atttcgaact caacgttctg accttcagcc agagttttga
     1981 agccattacc ctggatagcg gagaagtgta cgaacacatc tttgctgcca tcagccggag
     2041 taatgaagcc aaaaccttta gactcgttga accacttaac ctgaccttta atctttgcca
     2101 tttgaaaaat tccttagatt gttttcttcg cccgcaggca taacatagat aaaactgaca
     2161 cattactgca tgaggcacca atataaggct cggcagagaa gcggtattca acgtcaacgt
     2221 gtttactcag gacttcttta ctgaaaatgc cacacataaa cagaactgta cctcgtttaa
     2281 cccgaaatct tttatcacat acaacgttaa ttatggcaag ccatttttaa acatatctcg
     2341 atcagacgca caaatctcga tacgtcttcc actttttttg cacacttatg caacggaata
     2401 cgcgccgata tatcattgtg cttaaccttg ccagttcagg cagatactta aactggcgta
     2461 gtttctaaca tagttcaata agttcagttg ttccaggcca gagggagaag ttattacata
     2521 gtgcgtgcaa tatcacattt tctgctatgc aatgaataaa aagttatatc actttttctc
     2581 ataaaacagt cagttaacgg ctatcaatta ccctaaagag aagtcaatcc ccaaagggat
     2641 tgtaaattta aaataagaaa aattgatgag tgagcaaaaa aaatcaagag agaaacgttt
     2701 cgctaataaa taataccacg cttaatcaca aatctgatgt aaacatccta ataaattaat
     2761 gaggaaaata tctttttctg atatcccgtt acgcaaataa ataatatgat aattatttga
     2821 agtcagacca gagttttaat ttgaagcagt tgcaccatga cagtgcgtat aatcaggatg
     2881 aattgagtaa cccttcaacg acggggttac tcaagattga ggaaggatta tcgttcggcg
     2941 accaggcgaa taatatcttc gtcatcaacc gatttttgac tctcaatggt ctttttatct
     3001 gctttctctg gttcgtttgc ttcgcacaaa cgacgtaaca gtgcattttg acgtttttgc
     3061 tgatccagca acgcctcaag cagttcaatc tgttcgttag tccgtgaact ggcacgattg
     3121 ataaaaaacc acaagatgag cccgataaga agaaccacca ccgatacaac caaagacgca
     3181 atgttcatca cgcctgaatt tacaacttcg ttcatttcac cacctctgag tagaggcgct
     3241 attctaccac tgctggagag gaagaaaatc tagtgctgaa aaaatgatat caccacggga
     3301 taaactggtt aatggcacaa attccgctga aaaattgtac atcctgatcg cacatcatgt
     3361 tgaatacctg cgcccaaagc agcaagcaag ccaacaccac gacaaccaga acgacccatc
     3421 gaaacttttt cactccaatc tccgcttcac ctaccttatg tcattaaact agcatgcgtt
     3481 agttaaacag cgcctaacta tgtcgatatt cgtgcttttt cggaatgagt agcttgcttc
     3541 agcgcatatt gccgctacga ttaagcgaac ataaaaagag aagagattgt aatgcgattc
     3601 atcattcgca cagttatgct gatagcactg gtatggatag gattattact cagcggatat
     3661 ggcgttttga ttggaagcaa agagaatgcc gcaggattag gtttgcaatg tacctatctg
     3721 actgccagag gcaccagcac ggtacaatac ttgcacacta aaagtggttt cttggagata
     3781 acagattgcc cactgttacg taaaagcaat atcgtggtcg ataatggtta agggtaataa
     3841 ataaccagat tctcactcat gagtaaaata ttcttatagc aagcgcgttt aatataacgc
     3901 accttcaact ttatttttct catgctaaat aaagaacctg tctgtttgat attccgacag
     3961 gttcttactc tcatcagaac ggatagtcgt gataacccat ttgggcagaa attttgcgcg
     4021 ctgcggtatg caacattgcg acatattcct gtaaacgctc ttcagagaaa cgcaacgtcg
     4081 ggaaggagat gctcaaaccg gcaatgacca cgccaaagcg atcaaatacc ggtaccgcaa
     4141 tgcattgcag cccttcttcc tgctcttcgt tatcttctcc atacccctgc tcgcgcacct
     4201 ggtccagaac gggtaataac gcttctgtac tggtgatggt ccgctcggta ctgcgtttat
     4261 actccacgcc ctcaagaatt tgcttcactt catcgcgatc gcgccatgcc agcagtacct
     4321 taccaatcgc ggtgctgtac agcggattac gacgcccaat ccgtgaatac atgcgcaaat
     4381 tgtacataga gtcaattttg tgaatgtaaa caatactgtc ttcgtccagt gcgccgaggt
     4441 ggatagtttc tttggtcagg cgggagatct cacgcatctg gatatctgcg ctacgaatta
     4501 aatcgacgtt ttgtaacgcg cgagcgccca gttcaaacaa tttcagggtc agggaatatt
     4561 tctccgactc cccttcctgc gccacataac ctaaggtttt catggtctgt aaaaagcgat
     4621 aaacggtgct ttttgacatc atgacgcgct gcgacagctc ggttatccct atttcgcgct
     4681 cttcacccag cgcctgcaaa atgccaaaaa cttttagcac ggaagataca gaatcaggct
     4741 gtttatccag atctgcgtta gccatttatc acctcattgc gagtgtttta taaaaatcag
     4801 aactgttttt tattataatt tcgcaccagg gtggtcgcaa tccatctttt gccggttagt
     4861 tacaattctg cgacatccac cgtgaatatc agtgctagaa tcatacccct gttgattatt
     4921 caccaaagat ataaaattcc tatgccaaaa gttcaggccg acggcctgcc attgccccag
     4981 cgatacggtg cgatattaac cattgtgatt ggtatttcga tggctgtcct tgacggcgca
     5041 atcgccaacg tcgccctgcc aacaattgcc acggaccttc atgccacacc agccagttcc
     5101 atctgggtag tgaacgccta tcaaatcgcc attgtcatct ccctgctctc gttttcgttt
     5161 ctgggcgata tgtttggcta tcgacgtatt tataaatgcg gtctggtcgt ttttctgttg
     5221 tcttcactgt tctgcgccct ttctgattcg ctgcaaatgc tcacccttgc gcgtgtcata
     5281 caaggtttcg gcggtgcagc gttgatgagc gttaataccg cacttatccg cctgatctat
     5341 ccacaacgtt ttctggggag agggatgggc ataaactcgt ttattgttgc cgtctcttct
     5401 gctgccgggc cgacaattgc tgcagcaatc ctctccatcg catcctggaa atggttattt
     5461 ttaatcaacg taccgttagg tattatcgcc ctgcttctgg cgatgcgttt tctgccaccc
     5521 aatggttctc gcgccagtaa accccgtttc gacctgccca gcgccgtgat gaacgcgtta
     5581 accttcggcc tgcttatcac tgcgttgagt ggtttcgctc aggggcaatc gctgacgtta
     5641 attgctgcgg aactggtggt aatggttgtt gttggtattt tctttattcg ccgccagctt
     5701 tctcttcccg taccgctgct accggtggat ttactgcgta tcccgctgtt ttcactttct
     5761 atttgcacat ctgtttgctc tttctgcgca caaatgctgg caatggtttc cctgcccttt
     5821 tacctgcaaa ccgtgctcgg gcgtagtgaa gtcgaaacag gtttacttct gacaccgtgg
     5881 ccgttagcaa cgatggtgat ggctcctctg gcaggctatt tgattgaacg cgtacatgca
     5941 ggattgctgg gtgctttagg gttgttcatc atggctacgg ggcttttttc cctggttctg
     6001 ctgcccgcgt cacctgcgga tatcaatatt atctggccga tgatcttatg tggcgctgga
     6061 tttggcttat tccagtcacc caataaccac accattatta cctccgctcc gcgcgaacgt
     6121 agcggtgggg ccagtggcat gttaggaacg gctcgtctac tgggtcagag tagtggcgcg
     6181 gcgctggtgg cgctgatgct aaatcagttt ggtgataatg gtacgcacgt ctcgctgatg
     6241 gctgcggcta ttctggcagt gattgctgcc tgtgtcagtg gtttacgtat cactcagcca
     6301 cgagccaggg cataaaaaaa gcgcgtcgat caggacgcgc tttttagtat ttacttcata
     6361 ttacttcagg tattcacccg tacgcagagc ttcaatacgt ttatccagcg gcgggtgagt
     6421 catgaacaac tcactgagcg atttcgactt accgttaatg cagaaagcca tcatgctggt
     6481 tgcttcttgc ggttcatagc tggttttcag gcgctgcagc gcggcaatca ttttctcgcg
     6541 accaaccagt tttgccgaac cggcatctgc atggaattca cgatgacgcg agaaccacat
     6601 ggtgataatg ctcgccagaa taccaaacac cagttccaga accgttgcaa ccgcaaagta
     6661 gatcagcggg ttgccgttgc tctcttcacc ttcatcacga tttccgccca taaaacccgc
     6721 ggcaagctgc gccagaatac gggaaataaa gataacgaag gtgttcacca cgccctgaat
     6781 cagcgtcatg gtgaccatat caccattggc gatgtggctg atttcgtgag caattaccgc
     6841 ctcggcttca tccgggctca tgttctgcag caaaccggtg ctgacagcaa ccagagaggc
     6901 atcacggcgc gcaccggttg caaaagcgtt gatgtccggc gcatggtaga tagccacttg
     6961 cggcatagcg atccccgcct gacgagcctg ggttgctaca gtattgacca gccaacgttc
     7021 cctttcgtta cgcggttgct cgatcacttc cccgccaaca gatcgtaatg ccatccattt
     7081 ggacatcaga agcgaaacga aggaaccacc aaaaccgaac agcaaggcca tgatcatcag
     7141 cccctgaacg ctgctcgact gtatccctgt caggctcagt accagcccga aaacgaccat
     7201 tacggccagg ttcgttagca ggaagagcgc gattcgcatc ataattttct ttttacctca
     7261 gtttaacaaa acgcaatatg cgatacccac atcgtatggg ttacgcgact attttcaagt
     7321 ctggatagtg cgtaagtcac cagaaagaca caactttaca ttttgtagca tctgatttac
     7381 ggcatcttgc cgctgttaaa aaatcaggca caatttcttg tgcctgattg atattacttg
     7441 acgggagcgg gttgttccgc gggtctggct ttttcaagct tcgccagatc gagtgcgata
     7501 ttcaccgtct catccagata aggatccggc tcctggtaat ctttcgatag atcatccagc
     7561 ttcttcaact ccggtttacc ttctcgttta aagcgttcgt tcaaacgcgc cagacgcgtc
     7621 gcatcatctt cattattctc tttctcacgc acagcgtagt tcagagaaac gatattgcgc
     7681 ttgtccttca tagcgttgaa gcgcgcgata tccttcatga tgttctggaa ctcaggatct
     7741 ttcgcgatac gcgcattatg ttccttcagc agctccggtt caaaggcagt taaatctcct
     7801 gatttcacat aagtcgcggc atcgatgcta tcccacggca gcgcgttatc ttcgaatttc
     7861 tcacccgttt ccgtttcttc attacccgtc ggcatgatga tgtctggtgt tacgccttta
     7921 cgttgcgtac tgccgccgtt aacgcgatag aatttctgga tcgtgtactg cacagaaccc
     7981 agcgctggcc attcaggacg taacatctga tcgtaaatac ggttcaatga acggtattgc
     8041 tgaacggtgc ctttaccaaa cgtcggttca cccacaacca gcgcacgacc gtaatcctgc
     8101 attgccgcgg caaagatttc tgaagccgaa gcactgaaac ggtcaaccag taccaccagc
     8161 gggcctttat agaacacctg cccgtcggta tcgctgtctt cacgaacctt gccgttgtta
     8221 tcgcggacct gaacaatggg acccgcagga ataaacagac ctgagagcga taccgcttcg
     8281 gtcaacgccc caccgccatt gctacgcagg tcgatgatca cactgctgac attctgtttt
     8341 tccagtttct gcagttgaac tttgacatcg tctgtcaaac ccacatagaa gcccggaata
     8401 tccagcacgc cgactttctc tttaccgacg gtcttcaccg acattttaac cgcgcggtct
     8461 tcgagacgaa tacgttcacg ggtcaacgtt acagtacggg tcttggtccc tttaccagca
     8521 ggtaaaattt ccagacgaac tttactgccc ttcggccctt taattaaggc aaccacatca
     8581 tcaagacgcc agccaatcac gtcaaccatc ggcttgcctg tttgaccaac accgacaatt
     8641 ttgtcaccaa cgctgatagc tttactcttc gctgccggac cacctgccac catcgaattg
     8701 ataacggtgt agtcatcatc catttgcagc actgcgccaa taccttccag tgacaaactc
     8761 atttcagtgt tgaactgttc ggtattacgc ggggaaagat agttggtatg cggatcgatt
     8821 tcacgcgcaa acgccgtcat tgccagcgag aaaacatctt cgctgttggt ttgcgccaga
     8881 cgacgaatgg caaatttgta gcggcgtgtc agggtttcac gaatttcttt atccgttttt
     8941 cctgtcagct tcaggcttaa ctcgtcgaat ttgactttac tgtcccactg cgcgttcaac
     9001 tcagcctcgt ttttcggcca gggcgctttg ctgcggtcaa ggttataagt gtcgttgccg
     9061 gtgaaatcca tcggcttttc cagtaccgac aaagcgtact ggtaacgttc aaaacggcgc
     9121 ttttgcgcca gattgtagag atcgtagaaa acgtcgagtt tgcctgaacg cagttcatcg
     9181 cctaactcgg ttttcttttt cgcgaactgt tcaacatcgc ttgccagcag cacattgtgg
     9241 ctgtaatcga gcagattcag gtagcggtca aagattttgg ccgaaaatgc ctgatcgagg
     9301 tcgaactggc gataatgaga acgggtgaag cgcgacgtta cgcgctcact taccgtcgca
     9361 tgctgcgtct cttcctttaa taccggaatt tgatcagcac gcgtgatatc ttctacagcg
     9421 aaggtctggc ctgctattgc aagcaggcca gctaacgcgg taagcctaaa aaacatgttc
     9481 atgcctggcc cggcctccgt ttcagaacac caggtgttct gcgcgcacaa tcaaagacat
     9541 acccgaattc agctggacgc ggacgccgtc tttggtgatt tctaatacgg tggcatccat
     9601 cgcgttttga cccgctttca ccttcagggc ttgtccgaca gtcagagctg aaatgtcaga
     9661 aaccggggtg tgctgttctt cgcgaggtgc ttttactgtt tttggcgctt tctctaccgg
     9721 cttttgcgca cgaggtttac gttcagcgcc ttctttgcgg cgtggcgtgg tcggacgtgg
     9781 cttgcgttcg cggcgcggtg cgtcttcttt ctcaccagca gctgcggcag cttcgcgttt
     9841 tttcgcttgc tgttcagcac gctgtgcctg aacacgcgct ttcgcttctt caagctgctt
     9901 gcgagcatgc tctacatgtt gctcgtccag ctcaccgcat gggttgccgt caagatcgac
     9961 acgcgttgcg ccgggtttaa caccgtaaag ataacgccag ctcgaagtgt agagacgtaa
    10021 agcggatcgt aattgcgttt tgctcaggtt catttccccg gcaacacgat cgaccaaatc
    10081 ctgaaaaata ccaattttca gcggacgcgc ttcaccttcc gcactgaaac agtggggaaa
    10141 acgttcggcc agaaacgcga ttacttcttt actgctattc aacttaggtt gattttccat
    10201 gaaatttcct gattacaacg gacgtagcca acaagcgcag gcatgaacag gcgtcattat
    10261 aatgacgcta ccagtaaatg ctacgttatc cgttgattat cctgcgacgc tcgcaaagaa
    10321 ttttttgtaa tccgtcgttg caagcacttt ttcaagctgt gccacaagct gacgtaagcc
    10381 ttgctcgtcc tcgtctgtaa agcgagcgaa gacagtacta tcgatgtcga gaacaccaat
    10441 aatctgattt ttcaccacca gcggcagaac aatttcagaa ttactcgccg catcacaggc
    10501 aatatgcccg tcaaacgcgt gaacatcctc gatacgctgc acttgattgc gggcaaccgc
    10561 tgtgccgcac accccgcgcc cgacgggtat ccggacacag gcaattttgc cctgaaatgg
    10621 tccgagtacc agtgtatcgt cctcaagcaa ataaaaacct gcccagttaa cgtcagtgag
    10681 acgctcgtat aacaacgcac tggtgttcgc aagcgttgcc agaaaactgg tttctcccgc
    10741 catcagcgcg ttaaagtcgc gatttaaatc cgcgtaaaat tctgttttgt tcattactta
    10801 atcacttggt tgtcttacct ggaactgcca gcctattaaa ataagcatta aatgcgttaa
    10861 tgctcaagat cattcccatc atgggttaag attaatgtta attcttatta catttggcac
    10921 gtcatggctc ttaacacacc acaaattacg ccgacaaaaa agataacagt gagggcaatc
    10981 ggcgaggaac tgccgcgtgg tgattaccaa cgttgcccgc aatgtgacat gctgtttagc
    11041 ctgcccgaga taaattctca tcaaagtgcc tattgtccgc gctgtcaggc aaaaattcgc
    11101 gacgggcgcg actggtcgct aacgcgcctg gcggcaatgg ccttcaccat gctgttgctt
    11161 atgccgtttg cctggggcga accgctgttg catatctggc tgttaggcat tcgtattgac
    11221 gccaacgtca tgcaaggcat ctggcaaatg accaaacagg gcgatgcgat aacgggatca
    11281 atggtctttt tctgcgttat tggtgccccc ctcattctgg tgtcctccat agcatattta
    11341 tggtttggta accgactggg aatgaattta cgtccggtac tgctgatgct tgagcgactt
    11401 aaagagtggg taatgctgga tatctacctg gtcggcattg gcgttgcttc tataaaggta
    11461 caggattatg cccatatcca ggcgggtgtc ggcttgttct cttttgtggc gttggtgatt
    11521 ttaacgacgg tgacgttgtc acatcttaat gttgaggagc tgtgggagcg atattatccg
    11581 cagcgccccg ctacgcgtag ggacgagaaa cttcgtgtct gtcttgggtg ccattttacc
    11641 ggctatcctg atcagcgtgg tctctgcccg cgttgccata tcccgctacg cctgcgtcgt
    11701 cgtcatagtt tgcaaaaatg ctgggcggcg ctgttagcgt caatcgtttt gttgttacca
    11761 gccaacctat tgcctatttc tatcatttat ctgaatggtg gccggcagga agatacgatt
    11821 ctttccggaa ttatgtcgct ggcaagtagc aacattgcgg ttgcgggaat cgtgtttatc
    11881 gccagtattc tggtaccgtt tactaaagtg atcgtcatgt tcactttact gttgagcatt
    11941 cattttaaat gccagcaagg tttacgcaca cgcattctgt tactgcggat ggtgacctgg
    12001 attggacgct ggtcgatact cgacctgttt gtcatatctt taaccatgtc gctgattaat
    12061 cgcgatcaga tcctcgcttt tactatggga ccggctgcgt tttattttgg cgctgccgta
    12121 attttgacta ttcttgctgt ggaatggctg gacagccgct tactttggga tgcacatgag
    12181 tcaggaaacg cccgcttcga cgactgaagc gcagattaaa aataaacgcc gtatctcacc
    12241 tttctggctg ctgcctttca tcgcgctaat gattgccggt tggctgattt gggacagtta
    12301 tcaggaccgg ggtaataccg tcaccatcga ctttatgtcg gcggatggta ttgttccggg
    12361 ccgtacgcct gttcgttatc agggcgttga agtcggaaca gtgcaggata tcagcctcag
    12421 cgacgatctt cgtaagattg aagtcaaggt cagcatcaag tccgatatga aagatgcgct
    12481 gcgcgaagag actcagttct ggctgatgac gccaaaagca tcgttggcag gtgtctccgg
    12541 gctggacgcc ctcgtcggtg ggaactatat cggcatgatg ccgggtaaag gtaaagagca
    12601 ggatcacttt gtcgcgctcg atacccaacc gaaatatcgg ctggacaatg gcgatctgat
    12661 gatccacctg caagcccccg ctctcggttc gctgaacagc ggttcattgg tctatttccg
    12721 caagatcccg gtgggaaaag tctacgacta tgccatcaat cccaacaagc aaggcgtggt
    12781 gattgatgtc ctgatcgagc ggcgttttac cgatctggtg aaaaaaggta gccgtttctg
    12841 aaacgtttcc ggcgttgatg ccaacgtcag tatcagtggc gcgaaggtga aactggaaag
    12901 tctggcggca ctggttaacg gtgcgattgc cttcgattca cctgaagagt cgaaacctgc
    12961 cgaggcggaa gatacctttg gtctgtatga agatctggcc cacagccagc gtggagtaat
    13021 aataaaactg gaactgccga gtggagcagg attaaccgcc gactcgacgc cgttaatgta
    13081 tcaggggctg gaagtcggac agctgactaa actggattta aatcctggtg gtaaagtcac
    13141 tggggaaatg accgttgatc cctgcttcgt gaaaataccc gcatcgaatt acgcaacccg
    13201 aaattatccc ttagcgatgc taatctcagc gccctgctga ccggaaaaac ctttgagttg
    13261 gtgcccggcg atggcgagcc acgcaaagag ttcgttgttg tgccgggcga aaaagccctg
    13321 ctgcaagagc cggacgttct gacactgacc ctgacagcac cggaaagtta cggtattgat
    13381 gcgggtcagc cgctcattct tcacggcgtg caggtaggcc aggttatcga tcgtaaactc
    13441 accagcaaag gcgtcacctt taccgtcgcc atcgagcctc agcatcgaga actggtaaaa
    13501 ggcgatagca aatttgtcgt caacagccgt gtcgacgtga aggtggggct ggatggcgtt
    13561 gagtttctcg gtgccagcgc ctcagaatgg attaacggcg ggatacgtat tctgccgggc
    13621 gataaaggcg agatgaaagc cagctatcca ctgtatgcca atctggaaaa agcgctggag
    13681 aacagcctta gcgatttacc caccacaacc atgagtttga gtgcagagac gctgccggat
    13741 gtgcaggcag gatcggtagt gctgtaccgt aaatttgaag ttggtgaagt tattaccgtg
    13801 cgtccgcgag ctaacgcgtt cgatatcgat ctgcacatta aaccggaata tcgcaacctt
    13861 ttgaccagca acagcgtgtt ctgggcagaa ggcggggcga aagttcagct gaatggtagt
    13921 ggcctgaccg tacaggcatc cccgctctcc agagcattaa agggggccat tagcttcgat
    13981 aacctcagtg gtgccagcgc cagtcagcgt aaaggcgaca agcgaattct gtatgcttcc
    14041 gaaacagcgg cccgtgcggt tggcaggcag attacgcttc acgctttcga tgccggaaaa
    14101 ctggcggtcg ggatgccaat tcgctatctc ggtattgata tcgggcaaat ccagacgctg
    14161 gatctgatta ccgcacgcaa tgaagtacag gcaaaggcgg tgctctatcc ggaatatgtc
    14221 cagacctttg ctcgcggtgg tacgcgcttc tcagtggtca caccgcaaat ctcggcagct
    14281 ggcgttgagc atctagatac tatcctccag ccgtatatca acgtcgaacc aggccgtggc
    14341 aatcctcgtc gcgactttga attagaagag gccaccatta ctgattcgcg ttacctagat
    14401 ggtttaagca ttattgttga agcgccggaa gccggttcgt taggcatcgg tacgcctgtg
    14461 ctgttccgtg gtctggaagt aggtacggtt accggaatga cgctggggac attgtcagat
    14521 cgcgtgatga ttgcgatgcg catcagtaaa cgctatcaac acctggtgcg taacaattcc
    14581 gtcttctggt tggcatcggg ttacagtctg gactttggtc tgacgggcgg cgtagtgaaa
    14641 accggcacct ttaaccaatt tatccgtggc ggcatcgcct tcgccacgcc tccgggtacg
    14701 ccacttgcac cgaaagccca agaaggcaaa cacttcctgt tgcaggaaag tgaaccgaaa
    14761 gagtggcgtg aatggggaac tgcgcttccc aaataatgcc cactgctccg gcgtgcctgc
    14821 gccggagcgc ttatgctaaa ctgcgcgcct gtttttttgc cagtggtaca tgctcgtggc
    14881 ccaacacacc gtttatttcc cggacgcgtt tctgacacaa atgcgcgaag ctatgccttc
    14941 gacgctctca tttgatgatt ttcttgccgc ctgtcagcgc ccgttgcgcc gcagcattcg
    15001 cgttaatacg ctgaaaatct ccgttgctga tttcctgcaa ttaaccgctc cttatggctg
    15061 gacgcttacg ccaattccgt ggtgtgaaga aggtttctgg attgaacgcg acaatgaaga
    15121 tgcattgcca ttgggtagta ccaccgagca tttaagtggc ctgttttata ttcaggaagc
    15181 cagttcaatg ttgcccgtcg ccgccttgtt tgctgacggt aatgcaccac agcgggtgat
    15241 ggatgtcgct gctgcgccag gctcaaaaac gacgcaaatt gccgcgcgga tgaataacga
    15301 aggggcaatc cttgccaatg agttttccgc cagttgggta aaagtgttac atgccaatat
    15361 cagccgctgt ggcatcagta atgttgcgct cacacatttt ggtggccgcg tgtttggtgc
    15421 ggcagtgcca gaaatgtttg atgccatttt gctggacgct ccctgctccg gcgaaggcgt
    15481 ggtgcgtaaa gatcccgatg cgctaaaaaa ctggtcaccc gaaagcaatc aggaaatcgc
    15541 agctacccaa cgggaactga ttgacagcgc ctttcatgca ttacgtcctg gcggtacgct
    15601 ggtttactcg acctatacct taaaccggga agaaaacgaa gccgtttgcc ggtggctgaa
    15661 agagacttac cccgacgaag tagagttttt accacttggc gatctcttcc ctggtgcaaa
    15721 caaggcgctg accgaagaag gttttttgca tgttttccca caaatttacg actgcgaagg
    15781 cttcttcgtt gctcgtctgc gtaaaactca ggcgatcccc gccttacccg ccccaaaata
    15841 caaagtgggc aatttcccgt tcagcccggt gaaagatcgc gaagccggac aaattcgtca
    15901 ggcggctgca agtgttggct taaactggga tggaaacctg cgactctggc aacgcgacaa
    15961 agaactgtgg ttgttcccgg taggcattga agccctgatc ggtaaagtcc gattttctcg
    16021 cttagggatt aaacttgccg aaacgcataa caaaggttat cgctggcagc atgaagcggt
    16081 tattgcccat gcctcccccg acaatgtaaa cgcttttgaa ctgacaccgc aggaagcgta
    16141 ggagtggtat cgcgggcgcg atgtttaccc gcaagccgcg ccagtggcgg atgacgtgtt
    16201 ggttactttc cagcatcagc cgattggttt agccaaacgg attggttcgc gactgaaaaa
    16261 cagctacccg cgtgaactgg tgcgggacgg gaaacttttt accagtaacg catgacagcg
    16321 cacaaaaaaa gcgcactttt tgactggcac attcgccggc ctcaactagg ctgaaaaatt
    16381 gtgcgatcgg actggtcgta ctacaatcag cagctaaatg gagagcacga agatgaaaac
    16441 cagtgtgcgc ataggcgctt ttgaaatcga cgacggcgaa ttacacggtg aatcgccggg
    16501 tgatcgaacg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc aactggatgc
    16561 ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg tcctttaccg
    16621 tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat ccaaaaagaa
    16681 cccgtcggca tggcgggtta tttgtcctgg ttattccccc gttgtaaaat ctctcctaaa
    16741 cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact ttttgtttgc
    16801 tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga acagcagtgt
    16861 ctttattcta tgagcgatca acggatccgc catggcagtt gttttccgat tgaggatttt
    16921 atagatggtt tc
//
LOCUS       DAJTRM010000086        16709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRM010000086 DAJTRM010000000
VERSION     DAJTRM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.086"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..508
                     /locus_tag="OO322_003038"
     CDS             <1..508
                     /locus_tag="OO322_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5907830.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO322_003039"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO322_003039"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5907831.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OO322_003040"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OO322_003040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5907832.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OO322_003041"
     CDS             complement(2876..3073)
                     /locus_tag="OO322_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5907833.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO322_003042"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO322_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5907834.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO322_003043"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO322_003043"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5907835.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO322_003044"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO322_003044"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5907836.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO322_003045"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO322_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5907837.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO322_003046"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO322_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5907838.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO322_003047"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO322_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OO322_003048"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OO322_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5907839.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO322_003049"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO322_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5907840.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OO322_003050"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OO322_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5907841.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OO322_003051"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OO322_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5907842.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OO322_003052"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OO322_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5907843.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16709)
                     /gene="fepE"
                     /locus_tag="OO322_003053"
                     /pseudo
     CDS             complement(15766..16709)
                     /gene="fepE"
                     /locus_tag="OO322_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3861 a         4786 c         4374 g         3688 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgcct cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgcctag cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctag
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccatt
//
LOCUS       DAJTRM010000087        16389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRM010000087 DAJTRM010000000
VERSION     DAJTRM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.087"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO322_003054"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO322_003054"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5907844.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO322_003055"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO322_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5907845.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO322_003056"
     CDS             2339..2554
                     /locus_tag="OO322_003056"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907846.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO322_003057"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO322_003057"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5907847.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO322_003058"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO322_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5907848.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO322_003059"
     CDS             5920..6417
                     /locus_tag="OO322_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5907849.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO322_003060"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO322_003060"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5907850.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO322_003061"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO322_003061"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5907851.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO322_003062"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO322_003062"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5907852.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO322_003063"
     CDS             9102..9665
                     /locus_tag="OO322_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5907853.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO322_003064"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO322_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5907854.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARFGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO322_003065"
     CDS             complement(10257..11237)
                     /locus_tag="OO322_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5907855.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO322_003066"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO322_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5907856.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO322_003067"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO322_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5907857.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO322_003068"
     CDS             12540..12830
                     /locus_tag="OO322_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5907858.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO322_003069"
     CDS             12838..13431
                     /locus_tag="OO322_003069"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5907859.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO322_003070"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO322_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5907860.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OO322_003071"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OO322_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OO322_003072"
     CDS             15882..16385
                     /locus_tag="OO322_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5907861.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4218 c         4124 g         3988 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgtttcg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcatacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAJTRM010000088        15804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRM010000088 DAJTRM010000000
VERSION     DAJTRM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.088"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            450..1508
                     /gene="citC"
                     /locus_tag="OO322_003073"
     CDS             450..1508
                     /gene="citC"
                     /locus_tag="OO322_003073"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5907862.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1523..1819
                     /gene="citD"
                     /locus_tag="OO322_003074"
     CDS             1523..1819
                     /gene="citD"
                     /locus_tag="OO322_003074"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5907863.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1816..2724
                     /gene="citE"
                     /locus_tag="OO322_003075"
     CDS             1816..2724
                     /gene="citE"
                     /locus_tag="OO322_003075"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5907864.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2735..4267
                     /gene="citF"
                     /locus_tag="OO322_003076"
     CDS             2735..4267
                     /gene="citF"
                     /locus_tag="OO322_003076"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5907865.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4271..4821
                     /gene="citX"
                     /locus_tag="OO322_003077"
                     /pseudo
     CDS             4271..4821
                     /gene="citX"
                     /locus_tag="OO322_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4796..5674
                     /gene="citG"
                     /locus_tag="OO322_003078"
     CDS             4796..5674
                     /gene="citG"
                     /locus_tag="OO322_003078"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5907866.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5725..7187
                     /gene="citT"
                     /locus_tag="OO322_003079"
                     /pseudo
     CDS             5725..7187
                     /gene="citT"
                     /locus_tag="OO322_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7301..8107
                     /gene="rna"
                     /locus_tag="OO322_003080"
     CDS             7301..8107
                     /gene="rna"
                     /locus_tag="OO322_003080"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5907867.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8336..8746
                     /gene="rnk"
                     /locus_tag="OO322_003081"
     CDS             8336..8746
                     /gene="rnk"
                     /locus_tag="OO322_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5907868.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8977..10186)
                     /locus_tag="OO322_003082"
                     /pseudo
     CDS             complement(8977..10186)
                     /locus_tag="OO322_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10407..10835
                     /gene="uspG"
                     /locus_tag="OO322_003083"
     CDS             10407..10835
                     /gene="uspG"
                     /locus_tag="OO322_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5907869.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10956..12521)
                     /gene="ahpF"
                     /locus_tag="OO322_003084"
     CDS             complement(10956..12521)
                     /gene="ahpF"
                     /locus_tag="OO322_003084"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5907870.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12766..13329)
                     /gene="ahpC"
                     /locus_tag="OO322_003085"
     CDS             complement(12766..13329)
                     /gene="ahpC"
                     /locus_tag="OO322_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5907871.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13701..14447
                     /gene="dsbG"
                     /locus_tag="OO322_003086"
     CDS             13701..14447
                     /gene="dsbG"
                     /locus_tag="OO322_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5907872.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14657..15559
                     /locus_tag="OO322_003087"
     CDS             14657..15559
                     /locus_tag="OO322_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5907873.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
BASE COUNT         3926 a         4083 c         3822 g         3973 t
ORIGIN      
        1 aaaagatacg cagcggaaat gccagtcttt ggaaaaatgc aaactgttta ttctcgttaa
       61 gctgcaacat tgttttcctt gcgggttgtc ccgagcctct ttgacggatg taattaaaat
      121 gttgaatcaa aatacataga tattaattat gatcgtggca aattttattc attaaatcaa
      181 ttaaatcact taaacctgcg agtcatttcg taattacatt aattaaaaaa cttaaaacca
      241 ttaaataaat aaaaaaccac taactctatg tgaaataaat caaaatttca cgccgaaata
      301 ctccttagga tgtatagcaa aaggagaaaa agatatacct cgatcacccc cctttctccc
      361 aagtgaaaat aaaaggttat cagtttgcaa cattgaacaa cattcgttgc aaatcgataa
      421 caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc acccgcgtaa
      481 aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa aatgatttga
      541 gcgttgacac cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt
      601 gcggtggaat tgccggaaat attattaaat gcgttgctat cagtgaatcc gttcgcggtg
      661 aaggactggc gctgacatta gccactgaac tgataaacct cgcctatgag cggcacagca
      721 cgcatctgtt tatttatacc aaaaccgaat acgaggcgct gttccgccag tgcggttttt
      781 ccacgctgac ctgcgtaccc ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga
      841 aacgctatgc cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt ggctgcattg
      901 tgatgaacgc caatcccttt acgaatggtc accgttatct gattcaacaa gctgcggcac
      961 agtgcgactg gttgcatctg tttttagtca aagaagattc ttcacgcttc ccctatgaag
     1021 accggctgga tctggtgtta aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg
     1081 gctcagaata catcatctcc cgcgctacgt tcccttgcta cttcattaaa gaacagagcg
     1141 tcattaacca ttgttacacc gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg
     1201 cgcttggtgt aactcaccgc tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt
     1261 acaaccagga tatgcgctac tggctggaaa cgccgactat ctccgcagcg cccatcgaac
     1321 tggttgaaat tgagcggctg cgttaccagg agatgccgat atccgcttcc cgggtacgtc
     1381 aactgctggc gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc
     1441 attatttgca gaacctgctt gagcactccc gccaggacgc ggcagctcgt caaaagaccc
     1501 ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag gcacccttga
     1561 gtctggggat gtgatgatac gcatcgcccc actcgatacg caggatatcg acctgcaaat
     1621 caatagcagc gttgagaaac agtttggcga tgcaattcgc accaccattc tggacgttct
     1681 cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat gacaaaggcg cactggactg
     1741 cattttacat gcacgactgg aagccctgct ggcacgcgcc agcggtatcc cggctctgcc
     1801 atgggaggat tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc
     1861 agcatgttgt ttgtgcctgg tgccaatgcc gcgatggtca gcaactcctt catctacccg
     1921 gctgatgccc tgatgtttga cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc
     1981 cgccgcatgg tttatcacgc gctgcaacat ccgctgtatc gcgatattga aaccattgtg
     2041 cgtgtcaacg cgctggattc cgaatggggt gttaacgacc tggaagccgt cgttcgcggt
     2101 ggtgcggacg ttgtgcgtct gccgaaaacc gataccgctc aggatgttct ggatattgaa
     2161 aaagagatcc tgcgtataga aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg
     2221 gcggcgattg aatctccgct gggcattacc cgcgcagtgg aaatcgctca cgcttccgag
     2281 cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc
     2341 tccccggaag gaactgaact gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct
     2401 gcgggtattc aggcgttcga taccgtctat tccgacgcta acaacgaagc cggattcctg
     2461 caagaagccg cccacatcaa acagctgggc tttgacggca aatcgctgat caacccgcgt
     2521 cagattgatc tgctgcacaa cctctacgca ccgacccaga aagaagtgga tcacgcccac
     2581 cgcgtcgtag aagccgctga agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac
     2641 ggcaagatgg tggacggtcc ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa
     2701 ctttccggca tccgcgaaga ataaggcaat caaaatgacg cagaaaattg aacaatctca
     2761 acgacaagaa cgggtagcgg cctggaatcg tcgcactgaa tgcgatcttg ccgctttcca
     2821 gaactcacca aagcaaacct accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa
     2881 cctggaagaa gcgattcgtc gctcaggttt acaggacggc atgacggttt ccttccatca
     2941 cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg
     3001 ctttaaaaac ctgaccctgg cgtccagctc cctgagtgat tgccatgcgc cgctggtaga
     3061 acacattcgc cagggcgtgg ttacccgcat ttatacctct ggcctacgcg gtccactggc
     3121 ggaagagatc tcccgtggtc tgctggcgga accggtgcag atccactctc acggcggtcg
     3181 tgtgcatctg gtacagagcg gcgaactgaa tatcgacgtg gctttcctcg gcgtcccgtc
     3241 ctgtgatgaa ttcggtaatg ccaacggcta caccggtaaa gcctgctgcg gctccctcgg
     3301 ctatgcaatg gttgatgccg acaacgcaaa acaggtcgtg atgcttaccg aagaactgct
     3361 gccttatccg cataatccgg caagcattga gcaagatcag gttgatttga tcgtcaaagt
     3421 taatcgcgtt ggcgatgctg caaaaatcgg cgctggtact acccgtatga ccactaaccc
     3481 gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa
     3541 agaaggtttc tccatgcaaa ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct
     3601 ggaagacaaa atgcgtagcc gcgatattcg cgccgacttc gccctcggcg gtattaccgc
     3661 gacgatggtt gatctgcacg aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt
     3721 tgacagccat gctgcgcaat cgctggcccg taaccccaat cacatcgaaa tcagcgccaa
     3781 ccagtacgct aactggggtt cgaaaggcgc atcggttgat cgtctcgacg tggtggtact
     3841 gagcgcgctg gaaattgaca ggcagttcaa cgttaacgtg ctgaccggtt ctgacggcgt
     3901 actgcgtggc gcttccggtg gtcactgcga taccgcgatt gcctctgcgc tttccatcat
     3961 cgtcgcgcca ctggtacgcg gtcgtattcc gactctggtg gataacgtgc tgacctgcat
     4021 caccccgggc tccagtgtcg atattctggt cacagaccac ggtatcgcag ttaacccggc
     4081 acgtccggaa ctagcagaac gtctgcagga agcgggcatt aaagtggttt ccattgagtg
     4141 gctgcgcgaa cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg
     4201 cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa
     4261 ggaataagcc atgcacctgc ttcctgaact cgccagccac catgcggtgt caattcccga
     4321 gctgctcgtc agccgggatg aaaggcaagc acggcaacac gcctggctca agcaccatcc
     4381 tgttccactg gtctccttta ccgtggttgc gcctgggccg attaaagaca gcgaggtcac
     4441 acgccgaatt tttaatcatc gcgtgacagc cttgcgtgcc ttagccacaa agcagggctg
     4501 gcaaattcag gagcaggctg cactggtttc cgccagcggg ccggagggca tgttgagcat
     4561 tgccgccccg gctcgcgacc tcaagctcgc caccattgag cttgaacata gtcatcctct
     4621 cgggcggtta tgggatatcg atgtcctgac gcccgaaggc gaaattctct cccgccgcga
     4681 ctattcactg cgcctcgccg ctgcctgttg tgcgagcaaa gcgcagccgt ctgcgcgcgt
     4741 ggaaaaaccc atcaactgac cgatttactc aaccgcatgg aggcactgct gaacgatgtc
     4801 gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc
     4861 cctgctgggc tggcgcgcca tgctgactga agtcaatctg tcaccgaaac caggcctcgt
     4921 ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg gaagatttcc accgcagcgc
     4981 gctggcgatt cagggctggt taccccgttt cattgaattt ggtgcctgta gtgcagaaat
     5041 ggcaccggaa gcggtactcc acggattacg cccaattggt atggcttgcg aaggtgatat
     5101 gttccgcgcc actgcgggcg taaacacgca taaaggcagc attttttctt tagggctgct
     5161 atgtgcggca attggccgtt tgcttcagct caaccagccg gttacgccaa caaccgtttg
     5221 ttctacggcg gcaagtttct gccgtggcct gaccgatcgc gaactgcgta ccaataattc
     5281 acgactgacg gcaggtcaac ggttgtacca gcagcttggc cttaccggcg cacgcggtga
     5341 agccgaagca ggttatccac tggtgatcaa ccacgccttg ccgcattacc tcactctgct
     5401 ggatcagggg ttagatcctg aactggcatt gctcgatacc ttgctcctac tgatggctac
     5461 caacggcgat accaacgttg catcgcgcgg tggcgagggg ggcctgcgct ggctacagcg
     5521 cgaggcgcaa acattattgc aaaaaggggg cattcgaacc cccaccgatc tcgattatct
     5581 ccggcagttc gacagggagt gtatcgaacg aaatctcagt ccaggcggca gtgccgacct
     5641 gctgatcctt acctgttttt tagcacagat ttaattattt aagcacttga taaatttgga
     5701 aatattaatt ttcggagaac ccgtatgtct ttagcaaaag ataatatatg gaaactattg
     5761 gccccactgg tggtgatggg tgtcatgttt cttatccctg tccccgacgg tatgccgccg
     5821 caggcatggc attacttcgc tgtgtttgtg gcaatgattg tcggcatgat cctcgagcca
     5881 attccggcaa cagcgatcag ttttattgcg gttactattt gcgttattgg cagtaattac
     5941 ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa acaggcgctg
     6001 aaatggggcc tggctggttt ttccagcacc acggtatggc tggtatttgg cgcatttatt
     6061 tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca ttgccctttt cctggtgaaa
     6121 ttcatggaca aacgcacgct gacgttgggt tatgcgattg tcattatcga cattctgctg
     6181 gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc ggtcattaaa
     6241 aacctgccgc cgttgtttaa atcattcccg aacgatccgt ccgcgcgtcg tattggcggc
     6301 tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt tgtcaccggt
     6361 gcggaccaaa cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc cagattagct
     6421 ggttgcagtg gttcctctgc ttcctgccgg ttggggttat cttgcttatc attgcgccgt
     6481 ggctttccta cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg gcaacctggg
     6541 cgggtgatga actaaaaacc atgggtgcgc tgacacgcag agagtggacg ctgattggcc
     6601 ttgtattgct cagcttaggt ttgtgggtat ttggcagtga agtcattaat gctactgcgg
     6661 ttggtctgct ggcagtttcg ctaatgctgg ctctgcacgt tgtgccgtgg aaagacatta
     6721 cccgctataa cagcgcatgg aacacgctgg tcaacctggc aactctggtt gtgatggcta
     6781 acggcctgac tcgttctggt tttattgact ggttcgccgg taccatgagt acgcacctgg
     6841 aaggattctc accaaacgca acggtgattg tactggttct ggtgttctac tttgcacact
     6901 acctgtttgc cagcctgtct gcgcacaccg caaccatgct gccggttatt ctggccgtcg
     6961 gtaaaggtat tccgggcgta ccaatggaac aactgtgtat cctgctggtg ctgtctatcg
     7021 gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt tacggctgtg
     7081 gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat cttcggggtg atttacatct
     7141 ctatgttgct gttggttggc tggccgattc tcgccatgtg gaactaatac gttcgccaaa
     7201 cgctgaataa atcatcacgc ccgccaggta agcccacctg gcgggctttt ttatgattta
     7261 atagatagta ttcagtgtat gtatgagttc cacacccatt atgaaagcat tctgccgtaa
     7321 cgccgcgttg ctcgcggttt ctctgcttcc cttatcttct gccaacgccg tagcgttgca
     7381 ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt
     7441 ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga
     7501 aacgaccaac aaaactgatt ttctgaccgt acatggtctg tggccaggat tgcctaaatc
     7561 ggttgctgcc cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc
     7621 aatcccgaat ctaccagaag cacgcgccag ccgaatgtgt tcatcgccgg aaaccgggtt
     7681 atcactggaa acggccgcta aactaagtga agtcatgcca ggagctggcg gacgttcctg
     7741 cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc cggacgcata
     7801 cttcggtacg atggtacgcc tgaatcaaga aattaaagag agcgaagccg gaaaattcct
     7861 tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag
     7921 ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt
     7981 gactgaaatt cagatctcga tcaaagctga cgccatcaac gctccgcttt ctgcaaactc
     8041 attcttgcca caacctcacc caggtaactg tggcaaaacc tttgtgattg ataaagcggg
     8101 ttattaatcc tctgatttac aaaacacgct aattcgtctt ccataaccat gccgattaag
     8161 aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag
     8221 agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt
     8281 ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc
     8341 cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca
     8401 acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca
     8461 aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt
     8521 ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac
     8581 cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc tggggctgcg
     8641 cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct
     8701 ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc atctgcacaa
     8761 gcgataactc gctttgccca gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt
     8821 tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata
     8881 accatggcag aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc
     8941 atggctgtcc tttacctcga aaaccactcc tgacgatcat attgttcccc ccggcatcgc
     9001 attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag
     9061 aatcacctta cggcactcct cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc
     9121 ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa
     9181 cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc
     9241 tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac
     9301 gctaacaatg ccgcaacgct ttaccgctgc tgccctccag tttcaggtta gtcagcaccg
     9361 tttccgtggt gctgcctttc gcttcaaaac cgacggcgtc tattactgca tccacgcccc
     9421 ggtgacctgc cgtttgttca ataattgact gtgccggatc gctatcttca tcgaaattaa
     9481 tcgggatcgc gccgtagcgg tcagcggcga aacgcaagcg gtagggatga tgatcaacaa
     9541 caaaaatctg ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat cccacaggac
     9601 cagcaccata gactgcaacg cttgaacctt gttggatctg cacatttttt gctgcctgcc
     9661 atgccgttgg cagaatatca gaaaggaaaa gggctttatc atctgaaagc aaaggcggta
     9721 ctttaaacgg ccccacattc cctttcggga cgcggacata ttccgcctgc ccgccaggaa
     9781 cgccaccata caggtgacta taaccaaaca atgccgcggg agcaggtatc tgttttttat
     9841 tgagcgcagc gcctttaccc gcattggtat tttcgcaggc ggcatattgc tgtaatcgac
     9901 agaaaaaaca gtcgccacaa gcaatgacga acggaattac cacccggtcg cctttttgca
     9961 aattttttac gtccttcccg gtttcaacga cttcccccat aaattcatga ccaaaaatat
    10021 cgccatgttt aacctggggt attttgcctc gataaagatg gaggtcagag ccacagatcg
    10081 ccgttaccgt aatacgcaga ataatatcat ctgcctgttc aatgcccgga tcgggaacat
    10141 tttctacctg aacgtgatgt gggccgtgat aagtcaatgc tttcatgcaa cctccagaga
    10201 cagttcaaaa aagagtcagt ctttaagggt agctatcaca gcgggaaaac gggcggtacc
    10261 gcaggttttg ggagaattcc tagtgttcag tgagaatata ttaatcacag aaaccgttcg
    10321 taaggaaaaa gaaccagaca aatatcaggg ataaatatta taatcatgga gaatgatttg
    10381 ttcatgatta acagggagaa agggttatgt ataaggcaat cattatgcca gttgatgtat
    10441 ttgaaatgga attgagcgac aaagctgttc gccacgctga attcctcgcc caggatgacg
    10501 gagttattca tctacttcac gtactacccg ggtcagccag cctgagcctg caccgttttg
    10561 ccgctgatgt gcgtcgtttt gaagagcatc tgcaacatga agcagaagaa cgtctgcaaa
    10621 cgatggtcag ccacttcacc atcgatcctt cccgcattaa acaacatgtc cgttttggta
    10681 gcgtgcggga tgaagtcaat gagttggcag aagaactggg ggctgatgtt gtagttatcg
    10741 gttctcgcaa cccatcgatt tcgacccatc tgttaggttc taacgcctcg agcgtgatcc
    10801 gccacgccaa tctgcctgta ctggtcgttc gctaacagat aaaagtattt atgccgccct
    10861 gcctggaaca tcccggcagg gctttttaat gccataaaaa agccgccagg tttgaccccg
    10921 gcggctaagc aattgcaggt gaatcttact tcttcttatg cagttttggt gcgaatcagg
    10981 tagtcaaaag cactcagaga ggctttggca ccttcgccag tggcgatgat gatctgcttg
    11041 tacggaaccg tcgtacagtc acccgctgcg aacacgcctt tgacgttggt ttcacatttc
    11101 gcatcaatga taatctcgcc catgcggtta cgttcgactg cgccttccag ccagttggtg
    11161 ttcggcagca gaccaatctg gacaaaaata ccggccagtt cgatgttgtg aatattgccg
    11221 ctgacacgat ctcgatattc cagaccaacg actttgctgc cgtcgccttt cacttccgtg
    11281 gtttgcgcat tcagaataat gtcgacgttt ttcaggctgc gcagtttgtc ctgcagaacc
    11341 tggtcggctt tcatttctgg cgcaaattcc agcagcgtta cgtgctcaac gatacccgcc
    11401 aggtcgattg ccgcttccac gccggagtta ccgccgccga taaccgctac gcgtttgcct
    11461 ttaaacagcg ggccgtcgca gtgcgggcag taggtcacgc ctttagtgcg atactgatct
    11521 tcgcccggaa cgttcatgtt gcgccatttt gcacccgtcg ccacgataat gctgcgtgct
    11581 ttcagtaccg cgccagaagc tgtttcaatc tggtgtagac caccttcaac cgctgccggg
    11641 atcagtttgc tggcgctctg gctgtcgatc acatcaacgt cgtattcatc aacgtgaact
    11701 ttcagtgcgc ctgccaactt ctgcccttca gtcttcggta cagaaatgta gttttcgata
    11761 tcaacggtat cgaggatctg accaccaaaa cgttcgccca tcagaccggt acggatgcct
    11821 ttacgtgcgg agtaaattgc tgccgctgca cccgccgggc cggaaccaac gattaatacg
    11881 tcataagcat cacgcttgtt cagctcttct gccgcacgat tttccgcgcc agtatcaatt
    11941 ttggcaacga tttcagtcaa cgtcatgcgg ccctgaccaa actctttccc gtttacgaac
    12001 actgccggaa cgcccatcac gttgcgatcg gtgatttcgt tctggaaggt gccgccgtca
    12061 attgcagtgt gcttgatgcg cgggttcaat acgctcatca ggttcagcgc ctgcaccacg
    12121 tccgggcagt tatggcaaga gagcgagtaa taggtttcga attcaaaatc accgtcaata
    12181 tggcgaatct gctccagcag agactgcgct tctttcgacg gatgaccacc ggtccacagc
    12241 aacgccagta ccagcgaggt gaactcgtgg cccagcgggg agcctgcaaa acgtggtccc
    12301 tggttggaac ctgggttggt gatcaggaaa gacggcttac gcactggcaa gctgttatct
    12361 tctttaaagg tgactttgtc tgacagttct gcgatttcag ccaacagttc cttgatttct
    12421 gccgatttag cgctgtcatc cagcgtggca attaactcaa caggcttggt caatttctca
    12481 aggtaagcct tgagttgagt tttcatattt gtgtcgagca tgtttatctc ctgggcttta
    12541 aacatcatca tgcaagcggc ctctgcggct acctggatgc agcttgcatc atggtgcggg
    12601 gaagaaaatc gggtgccgca gcacccgaag aattaggtga atttccgaag tatttcgcgt
    12661 cagagcaagg cgactagcct gtgaatcccc gggagcttac acaagtaagt gaccggggtg
    12721 agcgggcgac gccaacgccg ctatggcgtg aaagacgaag gaaatttaga ttttaccaac
    12781 caggtccaga gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc
    12841 acctgggtga gaagctacgt actgagctgc tttgatttta cgcagcagat cagacgcgtc
    12901 acggccaatg ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac
    12961 gaaggtcgca cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag
    13021 ggcgccagtc gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct
    13081 gtgccatgct ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag
    13141 tttctgcagt tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt
    13201 aaagtcagcc gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat
    13261 ttcgatgaat tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat
    13321 caaggacatc tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttgccg
    13381 attcggctaa tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga
    13441 caactttgtg aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg
    13501 tgaccgtgta atctgaacta ccctttggta tcttcagcgc ctgcctgcag gctgaggtgt
    13561 tgcccttaca aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca
    13621 cctgatctaa gctcttacct atgacagtga taggttatgc cttttactcg acttttgcac
    13681 tgactgaaaa gaacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa
    13741 tcgccttcgc agaggaactt ccttccccag taaaagcgat tgaaaaacag ggcattacaa
    13801 tcatcaaaac gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata
    13861 tgggcgtcac catctacctg actcccgatg gtaagcacgc tatctctggt tacatgtaca
    13921 acgagaaagg tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg
    13981 gacgcgaaat gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg
    14041 cgccggtcat tgtctacgtc ttctccgatc cgttctgccc atattgtaaa cagttctggc
    14101 agcaggcgcg cccgtgggta gattctggca aagtgcaatt aagaacattg ttggttgggg
    14161 ttatcaagcc agaaagcccg gcgacagcag cggcaattct tgcctccaaa gatcccgcaa
    14221 aaacctggca acaatatgaa gcctctggtt gcaagcttaa gctaaacgtg cctgcaaacg
    14281 taagtacaga gcaaatgaaa gtgttaagtg acaatgagaa actgatggac gatctggggg
    14341 caaatgtcac gccggctatc tattacatga gtaaggaaaa tacgctgcaa caggccgtgg
    14401 ggttgcccga tcagaaaacg cttaatatca ttatgggaaa taaataaaag gtaattcatg
    14461 gatggttgaa ctatatccct gcttttcaac catcctaaaa aagcatgaat ttctcgctct
    14521 accctaagtg gctgtgctat tttagaaaaa cttaatcctg agttaagcaa aaaaacataa
    14581 tcaataaaaa tacatacgca aaaaacattg attaagtgaa tataccatgg aagaaaaata
    14641 taaaccggag tagtgtatgg ccaatctcta cgacttgaaa aagttcgact taaatcttct
    14701 tgtcatattt gagtgtattt accagcattt aagcattagc aaagctgctg aatcactata
    14761 tatcacccca tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat ttaatgaccc
    14821 tttattcatt cgctcaggaa aaggtatcgc gccgacgaca acagggttga atctgcatca
    14881 ccatcttgag aaaaacctca aaggccttga acaaacaatt aatatagtca ataagtccga
    14941 actaaagaaa aactttatta tctacggccc tcagctcatt tcttgctcta ataatagtat
    15001 gctgatccgc tgcctacggc aagatgcctc tgtcgagatt gagtgccatg atatcctcat
    15061 gtcagcagag aatgctgaag aactgttggt tcaccgcaaa gccgatctgg tgatcaccca
    15121 aatgccagtg atcagccgtt ccgtcatttg catgccattg catacaattc gcaatactct
    15181 aatttgtagt aataagcatc cgcgaattac tgacaatagc acttatgaac aaattatggc
    15241 agaagagttc acccaattaa tagccaaatc agcaggagtt gatgatattc aaatggaaat
    15301 agacgagaag tttatgaatc gcaaaatttc atttcgtggt tcctctttgc tgacaataat
    15361 taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt ataactacca
    15421 ccgtgacttt cttaatttaa aagagataaa actagaacat ccgttacctt caataaagct
    15481 ttacatttcc tataataaat cttctttaaa taatttggtg ttctcccgat ttattgaccg
    15541 tctaaatgag agtttttgat atataactat agacagctac tataactttc atctatttat
    15601 tcacagcgcg aatgaaaaat aaaacactct gctttacagt gtttacttta taattaacag
    15661 tcttatctca ctgttcaccc tgtaacgttc agagagcgtc ttcagatgtc tatttataaa
    15721 attcctcttc cgctcaatat tctggaagct gcacgagagc gtattaccta gacactcaac
    15781 accctacccc gcgtttgtgt ctca
//
LOCUS       DAJTRM010000089        15516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRM010000089 DAJTRM010000000
VERSION     DAJTRM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.089"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO322_003088"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO322_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5907874.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO322_003089"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO322_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5907875.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO322_003090"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO322_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO322_003091"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO322_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5907876.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO322_003092"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO322_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5907877.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO322_003093"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO322_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5907878.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO322_003094"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO322_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5907879.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO322_003095"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO322_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5907880.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO322_003096"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO322_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5907881.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO322_003097"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO322_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5907882.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO322_003098"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO322_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5907883.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO322_003099"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO322_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5907884.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO322_003100"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO322_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5907885.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO322_003101"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO322_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5907886.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO322_003102"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO322_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5907887.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO322_003103"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO322_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5907888.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO322_003104"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO322_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5907889.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO322_003105"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO322_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5907890.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO322_003106"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO322_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5907891.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO322_003107"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO322_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5907892.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO322_003108"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO322_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5907893.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO322_003109"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO322_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5907894.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO322_003110"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO322_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5907895.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO322_003111"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO322_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5907896.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO322_003112"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO322_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5907897.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO322_003113"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO322_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5907898.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO322_003114"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO322_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5907899.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO322_003115"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO322_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5907900.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO322_003116"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO322_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5907901.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO322_003117"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO322_003117"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5907902.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO322_003118"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO322_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5907903.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4005 a         3641 c         4130 g         3740 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcggcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagct
//
LOCUS       DAJTRM010000090        15334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRM010000090 DAJTRM010000000
VERSION     DAJTRM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.090"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(162..1187)
                     /gene="tdh"
                     /locus_tag="OO322_003119"
     CDS             complement(162..1187)
                     /gene="tdh"
                     /locus_tag="OO322_003119"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5907904.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="OO322_003120"
     CDS             complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="OO322_003120"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5907905.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="OO322_003121"
     CDS             complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="OO322_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5907906.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3829..4761
                     /gene="rfaD"
                     /locus_tag="OO322_003122"
     CDS             3829..4761
                     /gene="rfaD"
                     /locus_tag="OO322_003122"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5907907.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4771..5817
                     /gene="rfaF"
                     /locus_tag="OO322_003123"
     CDS             4771..5817
                     /gene="rfaF"
                     /locus_tag="OO322_003123"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5907908.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5821..6813
                     /gene="rfaC"
                     /locus_tag="OO322_003124"
     CDS             5821..6813
                     /gene="rfaC"
                     /locus_tag="OO322_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5907909.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6810..8018
                     /gene="rfaL"
                     /locus_tag="OO322_003125"
     CDS             6810..8018
                     /gene="rfaL"
                     /locus_tag="OO322_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5907910.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8054..9196)
                     /locus_tag="OO322_003126"
     CDS             complement(8054..9196)
                     /locus_tag="OO322_003126"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5907911.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9205..10218)
                     /locus_tag="OO322_003127"
     CDS             complement(9205..10218)
                     /locus_tag="OO322_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5907912.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="OO322_003128"
     CDS             complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="OO322_003128"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5907913.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="OO322_003129"
                     /gene_synonym="rfaI"
     CDS             complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="OO322_003129"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5907914.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="OO322_003130"
     CDS             complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="OO322_003130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5907915.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12816..13940)
                     /locus_tag="OO322_003131"
     CDS             complement(12816..13940)
                     /locus_tag="OO322_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5907916.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13937..14995)
                     /gene="rfaQ"
                     /locus_tag="OO322_003132"
     CDS             complement(13937..14995)
                     /gene="rfaQ"
                     /locus_tag="OO322_003132"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5907917.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4378 a         3444 c         3142 g         4370 t
ORIGIN      
        1 tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca gctatataaa
       61 agatcagcgc aattacgaac tctttattac actgacagga ttttacataa ttaacagatt
      121 atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca gctcagaata
      181 actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc atcgatagag
      241 aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc cgccatcttg
      301 taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc tttaaagatc
      361 actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc aatacgaccg
      421 ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga catttccaga
      481 ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag gttttctttc
      541 gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg gtattcgtta
      601 acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc agccataata
      661 cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa cgacagcgcg
      721 gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga gatattgtcg
      781 gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca gcccggacgg
      841 ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg acagttgcga
      901 cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt gaagcctttc
      961 acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac catcggcacc
     1021 gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt cccgcagatg
     1081 gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac agggacgtcg
     1141 gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc acatcctcag
     1201 gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg cgtaatttgc
     1261 tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt cggaacgacc
     1321 ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc aaatttctgc
     1381 gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc cgccagggta
     1441 aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag acggtcacgc
     1501 aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac aattgctggt
     1561 gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc aaccacttct
     1621 ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag cgtgccggtg
     1681 atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc attttcaccg
     1741 acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt atctgccaga
     1801 tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc atcggtggca
     1861 atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc aagctcctgc
     1921 atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc atcaataata
     1981 gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag cgtttcaaac
     2041 aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat ccccaggaat
     2101 gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat aaaacgcacc
     2161 gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat cagatccgga
     2221 tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct tccatcagcc
     2281 acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa caacccttcc
     2341 gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg cattgcgatt
     2401 ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg acgagatgat
     2461 gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc tctcaacacc
     2521 tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg attttactgc
     2581 attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca gttggcatca
     2641 aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc tcagcgcctg
     2701 catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc attttcctct
     2761 ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga caaaaatgcc
     2821 ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca gaactaattt
     2881 atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa tatatacatg
     2941 attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc ctttttttat
     3001 agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca atcctcgagt
     3061 tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg ttttcactaa
     3121 acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca aaacatactc
     3181 tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg taaatgactc
     3241 atctttttga attttttcga tacgacttct gatatatcta aatttctttt ttatatcgat
     3301 aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt tcaaatcggg
     3361 agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt aactaaaata
     3421 aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc aatcccctct
     3481 accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta ataagaccat
     3541 taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta tattatccat
     3601 tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac tattcacatg
     3661 caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt tgcaattagc
     3721 atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat gattacagac
     3781 attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat gatcatcgtt
     3841 accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga taaaggcatc
     3901 accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa cctggtggat
     3961 ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat ggctggcgaa
     4021 gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac caccgagtgg
     4081 gatggcaagt atatgatgga taacaactat caatactcca aagagctgct gcactactgt
     4141 ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg cggacgcacc
     4201 tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg ttactcaaaa
     4261 ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca gattgttggc
     4321 ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc
     4381 gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt cgaaggtagc
     4441 gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa cctgtggttc
     4501 ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga atccttccag
     4561 gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata cattccgttc
     4621 ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac aaatctgcgc
     4681 gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga atacatggcc
     4741 tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg cccgtcttgg
     4801 gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag
     4861 gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg gatgccggaa
     4921 gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc
     4981 aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt accaaactcc
     5041 ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc
     5101 gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc ctggccgcta
     5161 atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac cgcacaagat
     5221 ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa atcatatacc
     5281 tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc gggtgcggag
     5341 tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa gcagctgatt
     5401 gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc gggcaatgag
     5461 attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc gggggaaaca
     5521 cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac taacgattct
     5581 ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta tggtccaagt
     5641 agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct gattaccggc
     5701 tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt gatcgacatt
     5761 actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga agcctgacgg
     5821 atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac gttgcccgca
     5881 ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt ggaagaaggg
     5941 ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt ggcaatacgt
     6001 cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc gtttcgtgaa
     6061 gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg
     6121 gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca aaccgctcgc
     6181 gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca gcagcacgcc
     6241 gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc gcaaacccag
     6301 ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc tggcgaatat
     6361 gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga acactggcga
     6421 gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg gggcgcgccg
     6481 catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga agtattgccg
     6541 aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg
     6601 gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac ggtttatgga
     6661 ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg tcgttctaca
     6721 agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa tcttgaaata
     6781 ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc tctctcgaga
     6841 aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt accacctatt
     6901 ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca ataacgacaa
     6961 tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca tttctttttg
     7021 gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc cctgatgcag
     7081 gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta tgcacaatag
     7141 caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc gttttcttct
     7201 catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat tataaggact
     7261 atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct gactcgatgg
     7321 tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa taccgcattt
     7381 cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg
     7441 cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt
     7501 tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc acacataagg
     7561 agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa
     7621 atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt
     7681 acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga
     7741 cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt
     7801 tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa
     7861 atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt
     7921 ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg
     7981 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
     8041 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
     8101 catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
     8161 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
     8221 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
     8281 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
     8341 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc ttttcaggcg
     8401 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
     8461 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
     8521 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
     8581 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag atattaagtt
     8641 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
     8701 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
     8761 ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
     8821 taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc atgctattat
     8881 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
     8941 gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta tatatgcgac
     9001 tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt gagtatcctg
     9061 gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc tgatacatcc
     9121 acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta accgtaaata
     9181 ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa ttgctgtaaa
     9241 aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt tgtgctcatg
     9301 ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat aaatattacg
     9361 aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta taccagtata
     9421 atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat ttttttcttc
     9481 aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt tagcatgatg
     9541 tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta taattgactc
     9601 atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt cccgtaaatt
     9661 aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac acaaacgctc
     9721 agcgctttta ctttgcatag catctacatc aattaccact gccccatatt catctttaaa
     9781 ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat caagatataa
     9841 taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat aaatagatac
     9901 tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat gatataattt
     9961 aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact ctttgtctaa
    10021 gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa gaattgaagc
    10081 aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga cattaaacgt
    10141 ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt taaattgagt
    10201 aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc tcttcaatcc
    10261 tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata taagatatgt
    10321 atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat caatgcgatc
    10381 ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc ttaaaccttt
    10441 ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat gctgatgcaa
    10501 ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca aatactcatt
    10561 taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga aatttaatgt
    10621 tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac cacgtacccg
    10681 atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt ttaagaaacg
    10741 ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac gagcagtgtc
    10801 aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt taaccctgca
    10861 agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta tgaaaacttt
    10921 caacccattt atcgttttat tatatatcat aatgaattat tttaacctta aatctttaga
    10981 agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa tgcttttgtt
    11041 taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta agtaaatctt
    11101 ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc ggataatcag
    11161 cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg tcatcattga
    11221 ctggattatc aactttgtct tttaattcat agttaatact atatcgggta ttatatttct
    11281 ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga tcaaggtggg
    11341 tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac gaaatgttat
    11401 ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat ccagaagcta
    11461 actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct ctttcagcta
    11521 caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta atactaccct
    11581 tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag aaataatctg
    11641 caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt aatgacttca
    11701 gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt gctaaatcag
    11761 aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat tcacaagaga
    11821 tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa agaaaatttc
    11881 tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga taatgaaaat
    11941 tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt ctgtatccaa
    12001 aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt tccctgattt
    12061 ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga atgtctttaa
    12121 gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc aggccaatat
    12181 tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct ggaacgcgcg
    12241 tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct tccttaccgg
    12301 aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta atgcccgcag
    12361 catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt acgcgaacat
    12421 ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt ggtgtcagat
    12481 cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg ccgaatgcca
    12541 ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc cattcgcggt
    12601 ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct ttcagggtcg
    12661 tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc agagtacggc
    12721 gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca aaaggatctt
    12781 tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc acccgttatg
    12841 atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata atgtcgcgca
    12901 ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa aatctcattc
    12961 aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc gacaatataa
    13021 tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat cgcttccagc
    13081 agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc cgccgccatt
    13141 aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac gccgcgtttt
    13201 tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag caatgtgttg
    13261 tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc acctttacgc
    13321 gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc attcttctta
    13381 cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg ataaatcccc
    13441 ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa atcggcgatt
    13501 tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt tgcccgctca
    13561 aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt ttcctgcgct
    13621 actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg cattttgttg
    13681 aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca ggcaaaatac
    13741 tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac ttcaaataca
    13801 tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc tcgggcggcg
    13861 actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa atatttatat
    13921 aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt aatttatcga
    13981 cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt cggtcacgct
    14041 gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg ttatttgacc
    14101 agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc gtattaactg
    14161 cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca tgatcgatta
    14221 acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt ggcgtctggc
    14281 atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg gacgtcagaa
    14341 cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg gcgttgtccc
    14401 agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt ccaacaccag
    14461 cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc atggttgtct
    14521 gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt tccaccacat
    14581 ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca gactgccgat
    14641 gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt atagcaacca
    14701 tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat accttgatga
    14761 gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt atgccgtaga
    14821 gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa agcagcacat
    14881 cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta gttaataaca
    14941 tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta tccacaagta
    15001 actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct accgccccag
    15061 gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt gtatcacttc
    15121 tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca ataaagaaat
    15181 caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga ttccttcctg
    15241 cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag atgttcaaca
    15301 agaattggcg ggtaaattgc tatttaagtc aaaa
//
LOCUS       DAJTRM010000091        15323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRM010000091 DAJTRM010000000
VERSION     DAJTRM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.091"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..74)
                     /locus_tag="OO322_003133"
     CDS             complement(<1..74)
                     /locus_tag="OO322_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5907918.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO322_003134"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="OO322_003134"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5907919.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="OO322_003135"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="OO322_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5907920.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO322_003136"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="OO322_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5907921.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="OO322_003137"
     CDS             2704..2940
                     /locus_tag="OO322_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5907922.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="OO322_003138"
     CDS             2943..3800
                     /locus_tag="OO322_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5907923.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="OO322_003139"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="OO322_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="OO322_003140"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="OO322_003140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5907924.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="OO322_003141"
     CDS             complement(6415..7311)
                     /locus_tag="OO322_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5907925.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="OO322_003142"
     CDS             complement(7360..7599)
                     /locus_tag="OO322_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5907926.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="OO322_003143"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="OO322_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5907927.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="OO322_003144"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="OO322_003144"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5907928.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="OO322_003145"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="OO322_003145"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5907929.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="OO322_003146"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="OO322_003146"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5907930.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..12880
                     /locus_tag="OO322_003147"
     CDS             10766..12880
                     /locus_tag="OO322_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5907931.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12890..14182
                     /locus_tag="OO322_003148"
     CDS             12890..14182
                     /locus_tag="OO322_003148"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907932.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15132..15224
                     /locus_tag="OO322_003149"
     CDS             15132..15224
                     /locus_tag="OO322_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5907933.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
BASE COUNT         3516 a         4046 c         4031 g         3730 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg act
//
LOCUS       DAJTRM010000092        15311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRM010000092 DAJTRM010000000
VERSION     DAJTRM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.092"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1086)
                     /locus_tag="OO322_003150"
     CDS             complement(<1..1086)
                     /locus_tag="OO322_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5907934.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDA"
     gene            complement(1285..1369)
                     /locus_tag="OO322_003151"
     tRNA            complement(1285..1369)
                     /locus_tag="OO322_003151"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1333..1335),aa:Leu,seq:caa)
     gene            1565..2584
                     /gene="ahr"
                     /locus_tag="OO322_003152"
     CDS             1565..2584
                     /gene="ahr"
                     /locus_tag="OO322_003152"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5907935.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2713..4215
                     /locus_tag="OO322_003153"
     CDS             2713..4215
                     /locus_tag="OO322_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5907936.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4334..5416)
                     /gene="lptG"
                     /locus_tag="OO322_003154"
     CDS             complement(4334..5416)
                     /gene="lptG"
                     /locus_tag="OO322_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5907937.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5416..6516)
                     /gene="lptF"
                     /locus_tag="OO322_003155"
     CDS             complement(5416..6516)
                     /gene="lptF"
                     /locus_tag="OO322_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5907938.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6783..8294
                     /gene="pepA"
                     /locus_tag="OO322_003156"
     CDS             6783..8294
                     /gene="pepA"
                     /locus_tag="OO322_003156"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5907939.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8428..8871
                     /gene="holC"
                     /locus_tag="OO322_003157"
     CDS             8428..8871
                     /gene="holC"
                     /locus_tag="OO322_003157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5907940.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8871..11726
                     /gene="valS"
                     /locus_tag="OO322_003158"
     CDS             8871..11726
                     /gene="valS"
                     /locus_tag="OO322_003158"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5907941.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11780..12976)
                     /locus_tag="OO322_003159"
     CDS             complement(11780..12976)
                     /locus_tag="OO322_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5907942.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13169..13672
                     /locus_tag="OO322_003160"
     CDS             13169..13672
                     /locus_tag="OO322_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5907943.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13718..14134)
                     /gene="rraB"
                     /locus_tag="OO322_003161"
     CDS             complement(13718..14134)
                     /gene="rraB"
                     /locus_tag="OO322_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5907944.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14296..15309
                     /gene="argF"
                     /locus_tag="OO322_003162"
     CDS             14296..15309
                     /gene="argF"
                     /locus_tag="OO322_003162"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5907945.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3955 a         3899 c         3950 g         3507 t
ORIGIN      
        1 cgcatccctc gaccataatc ccgactcaat caacgaacta caagccatcg tcctgaagcc
       61 atgcccacaa acctcgacct tcgtgtcata ccccattacg cgcaaagcct tgttcaccgt
      121 attctcactc atcggcttac gtggatcgtg atcgcccaca aaaatcagct ctcgatttcc
      181 gctcatgctt ttgatctttt ccaaaatact tagggcctgc cgcgacaaag gtacaaggtg
      241 tggtgtccgc atcttcgagc cacgctgaga atgcttgact ccttccagtt gctcacgctc
      301 acctgggatt gtccacatag ccgtttcaaa atctatttct gaccagcggg caaaacgcag
      361 ctcgctcgaa cggatgaaaa ccaataaagt gagttcaacc gccagtcgag ttaacggtct
      421 gccagaatag tgatctatgc gatgaagtaa ttcaggtata cggttcagtt caagagctgc
      481 acggtgctgt cttttcgccg tagcaaccgc acctgcaatc tcttgcgcgg ggttgtagtc
      541 gattaagccg ctctgaacgg caaagcgcat aatcgcggta gtacgctgct gtaaacgggc
      601 ggcaacttcg agccgcccgg atgactcgac tgctttaatg ggtacaagaa gatcccgcgt
      661 cttcagttcc gcaatgttcc gcttaccgat agcagtaaag agattatcct ccagactttt
      721 caaaacacga gcgctatgcg atgccgacca cttctgattg ctggcgtgcc agtctctggc
      781 gactacttca aacgttatcg cctcttgttc ctgttctacc ttaacagctt tcttgttttc
      841 actgggatcg acaccattag ctaaaagctt acgagcctca tcccggcgtg ccctggcatc
      901 cgccaacgac acttcggggt atttccccaa tgccagcatc ttctctttgc cgccaaagcg
      961 ataacgtagc cgccagtatt tggagccgtt agggtgaacc agcaaaacca tgccttcacc
     1021 gtcggtcagc ttataggctt ttgcttcagg cttagccgaa cgaaccttca catcactcag
     1081 agccatgatg agtatccttt caagggttct gtgtgggtac aaacattatc gaaccgggaa
     1141 atacccgcag ttgtacccgc atcagtaagt tgatgtagat tgaatcaggt tgacttaggt
     1201 tgagtgaaaa agcgaggaaa gccttgcgga tactggattt taggcacaaa aaaagacgtc
     1261 cgttgacgtc tattgatgtt ccgatggtgc cgaaggccgg actcgaaccg gcacgtattt
     1321 ctacggttga ttttgaatca actgcgtcta ccgatttcgc cacttcggca ctgaagaggt
     1381 atgcggaaaa cgttgtggat tatacctgtc gcacgccacc atgcaagcgc cagcttgcac
     1441 ccacctcgct aagtgctgaa attttcagca ttacactcag cgatatgtaa cctttgtcac
     1501 actccaggca ccccgccctg ccatgctcta cacttcccaa acaacaccag agaaggcccc
     1561 aaaaatgtcg atgataaaaa gctatgccgc aaaagaagcg ggcggcgaac tggaagttta
     1621 tgagtacgat cccggtgagc tgaagccaca agatgttgaa gtgcaggtgg attactgcgg
     1681 gatttgtcat tccgatctgt cgatgatcga taacgaatgg ggattttcac aatatccgct
     1741 ggttgccggg catgaggtga ttgggcgcgt agtggcactc gggagcgccg cgcaggataa
     1801 aggtttgcag gtcggtcagc gtgtcgggat tggctggacg gcgcgtagct gtggtcactg
     1861 cgacgcctgt attagcggta atcagatcaa ctgcgagcaa ggtgcggtgc cgacaattat
     1921 gaatcgcggt ggctttgccg agaagttgcg tgcggactgg caatgggtga ttccactgcc
     1981 agaaaatatt gatatcgagt ccgccgggcc gctgttgtgc ggcggtatca cggtctttaa
     2041 accactgttg atgcaccata tcactgctac cagccgcgtt ggggtaattg gtattggcgg
     2101 gctggggcat atcgctataa aacttctgca cgcaatggga tgcgaggtga cggcctttag
     2161 ttctaatccg gcgaaagagc aggaagtact ggcgatgggt gccgataaag tggtgaatag
     2221 ccgcgatccg caggcactga aagccctgtc ggggcagttt gatctcatta tcaatactgt
     2281 gaacgtcagc ctcgactggc agccttattt tgaggcgctg acctatggcg gtaatttcca
     2341 tacggtcggt gcggttctca cgccgctgtc tgttccggcc tttacgttaa ttgcgggcga
     2401 ccgcagcatc tctggttctg ctaccggcac gccttatgag ctgcgaaagc tgatgcgctt
     2461 tgccgcccgc agcaaggttg cgccgacaac cgaactgttc ccgatgtcga aaattaacga
     2521 cgccattcag catgtacgcg atggtaaagc gcgctaccga gtagtcctga aagccgactt
     2581 ctgatccctt tgccgggtgc tgtcgcccgg cacttaaatc tctttggaag gcgtctcgca
     2641 atctgctgaa tttagcggtg aagagtgcac agccgttgcc tatactccag gcaggaaact
     2701 ggaggaaatc tcatgagcga acccctgtta attgcccgca cgccggacac agaactgttt
     2761 ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa
     2821 accgttacgc tgcaaaaact ggcagagtca ttgtcggaaa tcggcgtgcc ggtgtttatg
     2881 gctgatgtga aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc ggaaaaactg
     2941 ctcacaaggc ttaaaaatat cggcgtcaat gactggcaac cgcatgccaa tccggtggtg
     3001 gtgtgggata tctttggcga gaaaggccac acggtgcggg cgacggtttc ggatctgggg
     3061 ccgctgttgc tggcgcgact gttgaatctc aacgatgtgc aatctggcgt gctgaatatt
     3121 atcttccgca ttgcggacga tcagggattg ttgctgctcg actttaaaga tttgcgggcg
     3181 attacccagt acatcggcga taacgccaaa tccttccaga atcagtacgg aaatatcagt
     3241 agcgcatcgg ttggtgccat ccagcgcgga ttactgtcgc tggaacagca aggcgcagca
     3301 cacttctttg gtgagccgat gctggatatc aaagactgga tgcgcaccga tgccaacggt
     3361 aaaggcgtta tcaatatcct cagcgccgag aaactttatc agatgccgaa actgtacgcc
     3421 gccagcctgc tgtggatgct ttcagagttg tatgaacaat tgccggaagc gggcgatctg
     3481 gagaaaccaa aactggtgtt tttcttcgac gaggcacatc tgctgtttaa cgatgcaccg
     3541 caggtactgc tggataagat tgagcaggtg ataaggctta ttcgctcaaa aggcgtgggc
     3601 gtctggttcg tttcgcaaaa cccgtctgat attccggata atgtgttcgg ccagctcggt
     3661 aatcgtgttc agcacgcttt gcgggctttt acgccaaaag atcagaaagc agtgaagaca
     3721 gcggcgcaaa ccatgcgggc caatccggca tttgataccg aaaaggcaat ccaggaactg
     3781 gggaccggcg aggcgttaat ctcgtttctc gatgcaaaag gaagtccttc tgtggtggaa
     3841 cgggcgatgg tgatcgcacc ttgttcgcga atggggccgg tgacggaaga tgagcgtaat
     3901 ggcctgatta atcactcccc ggtgtatggc aaatatgaag atgaggtgga ccgcgagtcc
     3961 gcctatgaga tgctgcaaaa aggctttcag gccagtaccg agcagcaaaa taatcccccc
     4021 gtgaaaggga aagaggtggc ggtggatgac ggtattcttg gtggattgaa ggatattttg
     4081 tttggcacta ccggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggcgaaa
     4141 agcgccgctc gccaggtgac gaatcagatt gtacgcggga tgttggggag tttgctgggg
     4201 gggagaaaaa gataagtcag acgtgatctg tgcccctcac cctaaccctc tccccaaagg
     4261 ggcgagggga ccgttcgaac tcgtatttga ctttgtcaac aataataaag ggagctttcg
     4321 ctcccttgtt agattacgat tttctcatta acagccacag gctgattaag aagaagctgg
     4381 cacttggcaa cagtgcgccg atgatcggcg ggatgccgta aaccaacgtc agcgggccga
     4441 agatctggtc cagtacgtag aagacaaaac cgaagctgat acctgtgacc acacgcacgc
     4501 ccatcggtac gctacgcagt gggccaaaga taaacgacag cgccatcagc atcatcaccg
     4561 ccacggatag cggctggaag attttgctcc acatgttgag ctgataacgt ccggcatcct
     4621 gaccgctcga cttcagatac ttcacgtagt tgtgcaaacc actgatggag agtgcatccg
     4681 ggtccagcgc caccacgccc agtttgtctg gcgtgaggtc ggttttccag gtgccgctca
     4741 ccgtctgcga accggtaatc tgtttcggat tggtcagatc agattcatca acctgcgaca
     4801 gacgccagac tttatgttcc gggtcaaact tcgcggtagc ggcataacgt acggattgca
     4861 gacgacgatt ctcgttaaag gcataaatac tgatgccacc taacacctcg tcacctttaa
     4921 cccgctcaat gtagacgaag ttgttgccat ctttcgccca taaaccttgc tgggtagaga
     4981 gcaacgagcc gccgtacatc gcctgcgcac ggtagttacg cgccatctgt tcgccctgcg
     5041 gcgcgaccca ttcgccaatc gccatcgtca gcaagaccag tggaatggcg gttttcatca
     5101 ccgacagcgc tacctgcata cgggtaaaac cagaagcctg catcaccacc agttcgctgc
     5161 gctgcgccag catcccaaga ccaagcaacg ccccaagaag agccgccatc gggaagaaga
     5221 tctgcacatc tttcggcacg ctcagcaagg tatacattcc tgcgcctaac gcgtcgtaac
     5281 tcccctgccc ggcttttttc agctgatcga caaatttgat aatgcccgac agcgacacca
     5341 gcatgaacag cgtcatcatg atggtggtga aaatagtttt accgatatag cggtcaagta
     5401 cgccaaaagg ttgcatcaca ccgctccttt acgcgaaaaa ctggcgcgca ggcggcggac
     5461 cggcacggtg tcccaaaggt tgagaacaat cgccaaagcc agataaatca ggttaacggt
     5521 ccacatccac agtgtcgggt ccagcttacc tttaccgccg ttcgatttca gggaggtctg
     5581 gatcaggaag aaaagtagat acagcagcat ggctggcagc atcgacagta cgcgtccctg
     5641 acgcgggtta accacgctca acggtacaac cataagtgcc atcataaaca cggtgaatac
     5701 caacgtgata cgccagttca gttctgcgcg agcacgatcg gtgtcagtgt tccacaatgt
     5761 gcgcatgtcc atctggtcgg tatcgttcgg gtcgagcgcc accgcctggt gaccaatgat
     5821 cgcctgataa tcctggaaat ccgtaatgcg gaaatcacgt aacagtgcag tgccttcgaa
     5881 gcgcgttccc tggttgagag tgacgacctg ggagccgtcg cgcagctggg ttaaatgtcc
     5941 ggaatcggcc accaccacag aaggacgtgc attacctttt ggtcgaattt gtgcgaggaa
     6001 cacatctttg aaatcgctgc cgtcaacgct ttcgatgaac agcaccgagc tgccattagt
     6061 cgcttgctgg aattgccctt gcgccagcgc cgccatgcca gggttcgctt tcgcttctgc
     6121 taacacttca tcctgatgac gcgatgacca cggtcccgcc cacatcacgt taaccgccgc
     6181 gacgattgcc gtgaataccg caaggatcat tgccgctttc accagaaccg ctttgctcaa
     6241 gccgcaggca tgcattaccg taatttcact ttcggtatac agtttgccca gcgtcatcag
     6301 cagcccgagg aacaggctta atggcaggat aagctgcgcc atttccggca cgcccaaccc
     6361 gagaagggag agcaccagat tcgccggaat atcgccgtca accgctgcgc cgaggatcct
     6421 cactaacttt tgacagaaga agatcaaaag caagatgaag agtatcgcca gctggctttt
     6481 gagcgtctcc cgcaccagat atcttatgat tatcacttta aatacgcccg taaaaactcg
     6541 tcttttgcag gattttagct tgtttcatgg cttaaacgtc atttattctc ttgagtcgtc
     6601 gaaatcgtcg ctaagataat tatactcaac ggattcacct ctcagatttt gttctgacgt
     6661 gccaatgccg taataacgtt aagattaaca cgaagtcatc gcaacagcgg acatgagtta
     6721 cgaaagcttg caattctatc tgtagccacc gccgttgtct ttaagattca ggagcgtagt
     6781 gcatggagtt tagtgtaaaa agcggtagcc cggagaaaca gcggagtgcc tgcatcgtcg
     6841 tgggcgtctt cgaaccacgt cgcctttctc cgattgcaga acagctcgat aaaatcagcg
     6901 atgggtacat cagcgccctg ctacgccggg gcgaactgga aggaaaaccg gggcagacat
     6961 tgttgctgca ccatgttccg aatgtacttt ccgagcgaat tctccttatt ggttgcggca
     7021 aagaacgtga gctggatgag cgtcagtaca agcaggttat tcagaaaacc attaatacgc
     7081 tgaatgatac tggctcgatg gaagcggtct gctttctgac tgaactgcac gttaaaggcc
     7141 gtaacaacta ctggaaagtg cgtcaggctg tcgagacagc aaaagagacg ctctacagtt
     7201 tcgatcagtt gaaaacgaat aagagcgaac cgcgtcgtcc gctgcgtaaa atggtgttca
     7261 acgtgccgac ccgccgtgaa ctgaccagcg gtgagcgcgc gatccagcac ggtcaggcga
     7321 ttgccgccgg gataaaagca gcgaaagatc tcggtaatat gccgccgaat atctgtaacg
     7381 ccgcttatct cgcctctcag gcgcgccagc tggctgacag ctacagcaag aatgtcatca
     7441 cccgcgttat cggcgaacag cagatgaaag agctgggaat gcactcttat ctggcggtcg
     7501 gtcagggttc acaaaacgaa tcgctgatgt cggtgattga gtacaaaggc aacgcgtcgg
     7561 aagatgcacg cccaatcgtg ctggtgggta aaggtttaac cttcgactcc ggcggtatct
     7621 ccatcaagcc ttcagaaggc atggatgaga tgaagtacga tatgtgcggc gcggcggcag
     7681 tttacggcgt gatgcgtatg gtcgcggagc tgcaactgcc gattaacgtt attggcgtgc
     7741 tggcaggctg tgaaaacatg cctggcgggc gtgcctatcg tccgggcgat gtgttaacca
     7801 ccatgtccgg tcaaaccgtt gaagtgctga acaccgatgc cgaaggccgc ctggtactgt
     7861 gcgacgtgtt aacttacgtt gaacgttttg agccggaagc agtgattgac gtggcgacgc
     7921 tgaccggtgc ctgcgtgatc gcgctgggtc atcatattac tggtctgatg acgaaccata
     7981 atccgctggc ccatgaactg attgccgcgt ctgaacaatc cggtgaccgc gcatggcgct
     8041 taccgctggg tgacgagtat caggaacagc tggagtccaa ttttgccgat atggcgaaca
     8101 ttggcggtcg tcctggtggg gcgattaccg caggttgctt cctgtcacgc tttacccgta
     8161 agtataactg ggcgcacctg gatattgcag gaaccgcctg gcgttctggt aaagcaaaag
     8221 gcgcaaccgg tcgtccggta gcgttgctgg cacagttcct gttaaaccgc gctgggttta
     8281 acggcgaaga gtaagtttgc gttttaccct caccctaacc ctctccctaa gggagaggga
     8341 accgatcgag cgcaatagaa attcattgct catctttcag ggtgaggaat gcagcaatat
     8401 ttaaatccac cacaagaagc cccatatatg aaaaacgcaa cgttctacct tctggacaat
     8461 gacaccaccg tcgatggctt aagcgccgtt gagcaactgg tgtgtgaaat tgccgcagaa
     8521 cgttggcgca gcggtaagcg cgtactcatc gcctgtgaag atgaaaagca ggcttaccgg
     8581 ctggatgaag ccctgtgggc gcgtccggca gaaagctttg ttccgcataa tttagcggga
     8641 gaaggaccgc gcggcagtgc gccggtggag atcgcctggc cgcaaaaacg cagcagcagc
     8701 ccgcgcgata tattgattag tctgcgaaca agctttgcag attttgccac cgctttcaca
     8761 gaagtggtag acttcgttcc ttatgaagat tctctgaaac aactggcgcg cgaacgctat
     8821 aaagcctacc gcgtggctgg tttcaacctg aatacggcaa cctggaaata atggaaaaga
     8881 catacaaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact
     8941 ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat catgatcccg ccgccgaacg
     9001 tcaccggcag tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc
     9061 gctatcagcg catgcagggc aaaaacaccc tgtggcaggt cggtactgac cacgccggga
     9121 tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa acccgtcacg
     9181 actacggccg cgaagctttc atcgacaaaa tctgggaatg gaaagcggaa tctggcggca
     9241 ccattacccg tcagatgcgc cgtctcggca actccgtcga ctgggagcgt gaacgcttca
     9301 ccatggacga aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg tataaagaag
     9361 acctgattta ccgtggcaag cgcctggtaa actgggaccc gaaactgcgt accgctattt
     9421 ctgacctgga agtggaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc
     9481 tggctgacgg cgcgaaaacc gcagacggta aagattatct ggtggttgcg accactcgcc
     9541 cggaaaccct gctgggcgat accggtgtag ctgttaaccc ggaagatccg cgttacaaag
     9601 atctgattgg caaatatgtc attctgccgc tggttaaccg tcgtattccg atcgttggcg
     9661 acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa gatcacaccg gcgcacgact
     9721 ttaacgacta tgaagtcggt aaacgtcacg ccctgccgat gatcaacatc ctgacctttg
     9781 acggtgatat ccgtgaaagc gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt
     9841 attccagcga gatcccggca gagttccaga aactcgagcg ttttgctgca cgtaaagcag
     9901 tcgttgctgc ggttgatgca ctcggcctgc tggaagaaat taaaccgcac gacctgaccg
     9961 ttccttacgg cgaccgtggc ggcgtggtta tcgaaccgat gctgaccgac cagtggtacg
    10021 tgcgtgccga tgtactggcg aaaccggcag ttgaagcggt tgagaacggc gacattcagt
    10081 tcgtaccgaa gcagtacgaa aacatgtact tctcctggat gcgcgatatt caggactggt
    10141 gtatctctcg tcagctgtgg tggggtcacc gtatcccggc atggtatgac gaagcgggta
    10201 atgtttatgt tggccgcaac gaagaagaag tgcgcaagga aaataacctc ggtgccaacg
    10261 ttgccctgcg tcaggacgaa gacgttctcg acacctggtt ctcttctgca ctgtggacct
    10321 tctctaccct tggctggccg gaaaataccg acgccctgcg tcagttccac ccaaccagcg
    10381 tgatggtatc tggtttcgac atcatcttct tctggattgc ccgcatgatc atgatgacca
    10441 tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc gttccacact gtttacatga
    10501 ccggtctgat tcgtgacgac gaaggtcaga agatgtccaa atccaagggt aacgttattg
    10561 acccgctgga tatggttgac ggtatttcgc tgccagaact gctggaaaaa cgcaccggca
    10621 atatgatgca gccgcagctg gcggacaaaa tccgtaagcg caccgagaaa cagttcccga
    10681 acggcattga gccgcacggc accgatgccc tgcgcttcac cctggcggcg ctggcgtcta
    10741 ccggtcgtga catcaactgg gatatgaagc gtctggaagg ctaccgtaac ttctgtaaca
    10801 agctgtggaa cgccagccgc tttgtgctga tgaacacaga aggtcaggat tgcggcttca
    10861 acggcggcga aatgacgcta tcgctggcgg accgctggat tctggcggag ttcaaccaga
    10921 ctatcaaagc gtaccgcgaa gcgctggaca gcttccgctt cgatatcgcc gcaggcattt
    10981 tgtatgagtt cacctggaac cagttctgcg actggtatct ggagctgacc aagccggtaa
    11041 tgaacggtgg aacagaagcc gaactgcgcg gtactcgcca tacgctggtg actgtactgg
    11101 aaggtctgct gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc
    11161 gtgtgaaagt actttgcggt atcactgccg acaccatcat gctgcagccg ttcccgcagt
    11221 atgatgcatc tcaggttgat gaagccgcac tggccgacac cgagtggctg aaacaggcga
    11281 tcgttgctgt acgtaacatc cgtgcagaaa tgaacatcgc gccgggcaaa ccgctggagc
    11341 tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac cgtggcttcc
    11401 tgcaaacgct ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgac aaaggtccgg
    11461 tttccgttac caagatcatc gacggcgcag agctgctgat cccgatggct ggcctcatca
    11521 acaaagaaga tgagctggcg cgcctggcga aagaagtggc gaagatcgaa ggtgaaatca
    11581 gccgcatcga gaacaagctg gcaaatgaag gctttgtcgc ccgcgcaccg gaagcggtta
    11641 tcgcgaaaga gcgtgagaag ctggaaggtt atgcagaagc gaaagccaag ctgattgaac
    11701 agcaggctgt tatcgccgca ctgtaatcaa cacagtgatg aaaacgaagg ccggagcatg
    11761 ctccggcctt ttttatctct tacagaaatg gtaaagaagg cataactccc cgagaaatac
    11821 tggctaagaa gcctttatct ggttgttctt ctttattgat taaaggaagg tcatccaaat
    11881 cgcccagcaa atacgtattt tttgcctgcc agtcaatcat ccatattttc agcagtgggt
    11941 aagccagacc acccgtaatc ccggatatca ccaccaacgc acacatgcga taaagcatac
    12001 cgtggtacgt taaagttgag cgaaaacgaa tacgcccatc attcagtgac aggttgctca
    12061 taaaatgatt tcgcaacgac accgttaaat aactggtgct aacagcaatc ccaaaataat
    12121 aaattaactg cgcgattatc attttacgtt gcatttccat aaattgctgt aaattctcaa
    12181 tatcatcatt tgcataccca gaactatcat actcatttaa tatttgatcg aagataatat
    12241 aaccagcaac agcgagaaaa ggtaataaag ctaaaaatgc gaggatggca tattttatac
    12301 aatacgcagt atctaatttc accttgaaac gatgtatacc gaaactggta ttgctccaga
    12361 gaaaactcat taccaggcta tataaagtac cattaaaaat accaatggaa acaataccaa
    12421 ctattgccat cagaaccaca gatataataa cactacttga actattggca tgtagcatct
    12481 ttgtcgagat aaagaaaaca gtccccatcc caatggccat taaaatcggc aagaaaaagg
    12541 ttacccacca gaaccctttc aaagagcaat taaaactaaa tcttacgccg ttgagactgg
    12601 tcattaaggc ctgataacgc agtccttttg ctgccataaa tataagcaaa accaacagta
    12661 acaaagtcaa aacacagcca acaagcggca tatctgctga cactgtcatt aagattgcga
    12721 aatagaaaaa aacaaaaaca agacaactaa caaaaacatt cccaccggta attccataag
    12781 aaaatcgttg tccgttaact tccatattag cataaagata acgcttacat ttcattaatg
    12841 cccatggtaa atagatacct agcgttataa tcgttaacaa cacattcacc acacaaataa
    12901 gaaaatatct ctcacctgta ccatgaaaaa caaaagaatg ggacggaaca tccatttcat
    12961 taataacttg agccataaaa atcccctgaa ttaaaaaatc cattgatata atttatagat
    13021 acagacagta aaatgttctg ccacgttaca atactcttat cagtaatagg ctggcaaaaa
    13081 ctttattgct ttataagacg cttgcgcacg taagaagaga gtggtattaa acaaatgtat
    13141 aattacttta tttttattgg gttaaactat gaataacatt gcgccgcaat cacctgtaat
    13201 gcgtcgcctg acgctgcagg ataatcctgc tatcgccagc gtcatccgtc aggtttacgc
    13261 agagtatggt cttaccgccg ataaaggcta caccgttgcc gatccgaatc tcgacgaact
    13321 gtatcaagtg tacagccagc ctggccatgc ttattgggtg gttgaataca atggcgaagt
    13381 ggtcggcggc ggcgggattg cgccattaac aggtagtgaa gcggacattt gcgaactgca
    13441 gaaaatgtac tttcttcccg ctattcgcgg taaagggcta gcgaaaaaac tggccttaat
    13501 ggcgatggag caggcacgag agatgggctt caaacgctgc tatctggaaa cgaccgcttt
    13561 tttaaaggaa gccattgcgc tttatgagca tttgggcttt gagcatatcg actatgcgct
    13621 tggctgcacg ggccatgtcg attgtgaagt gcggatgcta cgcgaactct aaaaaaatgc
    13681 ccgctagtat gtgctatcgg gcattttaat caattaatta gtggcgaacc ccgtcatcgt
    13741 cttcatcgac aaaatcttca tcgtcgccat cttcgccgtt aggatcttca aagtaagtgc
    13801 cccaaccgtc gtactcaacg tcaaatttct ccgccagcgt catcagttgt tcaacctggg
    13861 catcgatcag atcggcattc aacgcgcact cgctgaggat gtcgcagcaa atcacgatat
    13921 caccatcttc aacttccagc tcttctggat cggtcacttc gtaaccgagt ttaaacgctt
    13981 caactgccgc tttttccaga gtttccagat cgtctgcgga aagatggtgt tcgatggtgt
    14041 acagcgcgtc cggatcgctg ccatcttcca gtaattcttc aataatcaaa cgtgtttctt
    14101 cacgctgttc ttccagttgt tccgggtttg ccatggctca ttcctcttaa tgtcctgccg
    14161 atactcttat tgtcacacac cactgacatt gcctccacct ttgtgacaaa gatttatgct
    14221 ttagacttgc aaatgaataa taatacacat aaattgagtt ttaattcatt gaggcgttag
    14281 ccacaggagg gatctatgtc cgggttttat cataagcatt tcctgaaatt actcgatttc
    14341 acgccagctg aactcaacag cctgctgcag ttagccgcga agctgaaagc tgataagaaa
    14401 agcggtaaag aagaagccaa actcactggt aaaaacatcg cgctcatctt cgaaaaagac
    14461 tcgactcgta cccgatgctc tttcgaagtt gccgcatatg accagggggc tcgcgttact
    14521 tatcttggcc caagcggtag tcagattggt cataaagagt cgattaaaga cactgcccgc
    14581 gtgcttggtc gcatgtatga cggtattcag tatcgcggct atggtcagga gattgtcgaa
    14641 acactggcgg aatacgctgg cgtgccggta tggaacggcc tgaccaatga gtttcacccc
    14701 acgcagttgc tggcggatct cctcaccatg caggagcatt tacccggtaa agcattcaac
    14761 gaaatgacgc tggtctatgc aggtgacgca cgtaacaaca tgggcaattc gatgctcgaa
    14821 gctgcggcgc ttaccggtct ggatttgcgt ctggtcgcgc cacaggcatg ctggccagaa
    14881 gctgcgctgg ttacggaatg ccgcgccctg gcacaacaaa atggcgggaa tattacgctg
    14941 acggaagatg tagctaaagg agttgaaggt gctgacttta tctataccga tgtatgggtg
    15001 tcgatgggtg aagcaaaaga aaaatgggct gaacgcattg cattactgcg tgattatcag
    15061 gtgaacagca agatgatgca gttgaccggt aacccggagg tcaaattcct ccattgcctg
    15121 cccgcgtttc atgacgacca aacgacgctt ggcaaaaaaa tggcggaaga gtttggccta
    15181 catggcggaa tggaagtgac tgatgaggtc ttcgaatctg ccgccagcat tgtatttgat
    15241 caggcggaaa accgcatgca taccatcaaa gcggtgatgg tcgcgacgct cagtaaatta
    15301 aataattaat a
//
LOCUS       DAJTRM010000093        14978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRM010000093 DAJTRM010000000
VERSION     DAJTRM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.093"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(520..1461)
                     /gene="fabY"
                     /locus_tag="OO322_003163"
                     /gene_synonym="yiiD"
     CDS             complement(520..1461)
                     /gene="fabY"
                     /locus_tag="OO322_003163"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5907946.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="OO322_003164"
     CDS             complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="OO322_003164"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5907947.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1940..2812)
                     /locus_tag="OO322_003165"
     CDS             complement(1940..2812)
                     /locus_tag="OO322_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5907948.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="OO322_003166"
     CDS             complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="OO322_003166"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5907949.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3504..4289)
                     /locus_tag="OO322_003167"
     CDS             complement(3504..4289)
                     /locus_tag="OO322_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5907950.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="OO322_003168"
     CDS             complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="OO322_003168"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5907951.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5387..6283
                     /gene="yihU"
                     /locus_tag="OO322_003169"
     CDS             5387..6283
                     /gene="yihU"
                     /locus_tag="OO322_003169"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5907952.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6307..7185
                     /gene="yihT"
                     /locus_tag="OO322_003170"
     CDS             6307..7185
                     /gene="yihT"
                     /locus_tag="OO322_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5907953.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7202..8443
                     /gene="yihS"
                     /locus_tag="OO322_003171"
     CDS             7202..8443
                     /gene="yihS"
                     /locus_tag="OO322_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5907954.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8581..9483
                     /locus_tag="OO322_003172"
     CDS             8581..9483
                     /locus_tag="OO322_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5907955.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9682..11718
                     /gene="yihQ"
                     /locus_tag="OO322_003173"
     CDS             9682..11718
                     /gene="yihQ"
                     /locus_tag="OO322_003173"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5907956.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11764..13138
                     /locus_tag="OO322_003174"
                     /pseudo
     CDS             11764..13138
                     /locus_tag="OO322_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13181..14584
                     /locus_tag="OO322_003175"
     CDS             13181..14584
                     /locus_tag="OO322_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5907957.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14652..>14978
                     /gene="ompL"
                     /locus_tag="OO322_003176"
     CDS             14652..>14978
                     /gene="ompL"
                     /locus_tag="OO322_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5907958.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3673 a         3855 c         3736 g         3714 t
ORIGIN      
        1 tgatttaata gcgtttgctt cggtgattta ttactcatta gttgtttttc taaattaaca
       61 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      121 cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt aaatgaatga
      181 tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta tagattttaa
      241 aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa atcgctcaaa
      301 cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag ctctacaaat
      361 aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca acgaagaata
      421 aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat gtaaatgacc
      481 ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt cgttcccgcc
      541 ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc
      601 acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg
      661 cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat ggggtttacc
      721 gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg
      781 gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca gcgagaataa
      841 actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca tggtggtgat
      901 aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa gcgggatatg
      961 ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa tatcatccag
     1021 agtggcgacg ggcttaatca tcaaaaaatg gcgaatcggc gtggtggttg gcgtggtgat
     1081 ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt cacgggcgct
     1141 acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca tcgccatcag
     1201 cgttcctaac cctttgtcct gcacgtccgg gtgaacgacc ataaagcgaa tggacgcttc
     1261 attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct cgtcgacgac
     1321 catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg gttgatgcag
     1381 gggcttacgc aacatttccc agcgaaagtg atagtaacgc tctaactctt cttctgtttg
     1441 tggaacctga aggtgataca tagcgatact ctctcttgtt tcccgtgaca accctggaag
     1501 ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga tacctgcata
     1561 tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg ttcgacgaaa
     1621 tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact tgggcgcatc
     1681 ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact gccgcccgcc
     1741 tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta gccgagcaca
     1801 cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc caataacacc
     1861 aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct ggcacgggtt
     1921 acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct gcttgtttta
     1981 gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg atacaccatg
     2041 tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa atgagctgat
     2101 atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg aacaggagtg
     2161 cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc
     2221 tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc agcacgttgt
     2281 cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa ctgatcgcca
     2341 gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg gcgaacgagt
     2401 aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta tcgatggagt
     2461 acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc atcttgttgg
     2521 aattagcaac aaattgttcg atataccgct gaataacatc gccagtagca ggcagaaagt
     2581 tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga aaagcggcga
     2641 aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca taggcaaggt
     2701 tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt tttagccagg
     2761 cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc ataacacctt
     2821 cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa tgcctagctg
     2881 attagctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa
     2941 accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca tccccagatc
     3001 ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt ccggccagaa
     3061 ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct gctcacgcag
     3121 tttatgcatg atggcgatca cttccgggcg cagcgcaaca aacaccgcct gccagccgtg
     3181 agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct ctgcgaacgc
     3241 ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat gaaaactctt
     3301 cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca cacggttaaa
     3361 gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg cctcctttcg
     3421 cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc aaggtatgaa
     3481 gccaggttaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat ttcaatttgg
     3541 ctgtcttgta agcgtgattt tagcgcagcg ggcggcagct cgtcggtaaa gagcgcagtg
     3601 acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt gtgatcggcg
     3661 accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc ttcgtttaca
     3721 tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac cagataatca
     3781 gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct attatgagag
     3841 cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag aatatgcgcc
     3901 acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc ccgggcaaca
     3961 tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat atagtctgcc
     4021 accgcttcgg caatcgcttt ttttttctcg gtttgcgaaa cctcacgctg ctcgaacgcc
     4081 gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc
     4141 tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag cagatttgcc
     4201 agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc
     4261 gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat caccctggca
     4321 ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat cccggctcgt
     4381 ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac tgactccgct
     4441 aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc tcccgcacct
     4501 gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc attttccagc
     4561 cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag cgtttgtgcc
     4621 tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc cggttctgaa
     4681 aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt aatgtccccg
     4741 tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt agcgccgtcg
     4801 tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc ctccaaccac
     4861 tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc gcctttggta
     4921 tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt gtaacgggtg
     4981 ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgcgtcgtc atcacctacg
     5041 cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc cgtcgccgct
     5101 ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc cgtcggtaac
     5161 ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac acgaatcatg
     5221 gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt ttaaaaagtg
     5281 acggctgtca attttttgac gataaattac aaattcgatc aaaaacagac aagcaaagca
     5341 tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg cagcaatcgc
     5401 gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc agcaagggca
     5461 tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag acaaaggtgc
     5521 gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca ttaccatgtt
     5581 gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt gcgaaggctt
     5641 atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa ccgataaatt
     5701 gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag gccgtacttc
     5761 tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg aacaagttga
     5821 acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaacgctg gcggtccggg
     5881 catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca atgcgctttc
     5941 ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt
     6001 gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa acaaagtcct
     6061 cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc ttggcatcgc
     6121 ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt
     6181 ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca
     6241 ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg ataaaggaat
     6301 aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg ttttgccatg
     6361 ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg ggcacccgct
     6421 ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc cctctcgcct
     6481 tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt tgagcaaaac
     6541 gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc tggcaacggt
     6601 attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat caaacaagac
     6661 ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc gcagcaacgt
     6721 ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt aagcatcatt
     6781 gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca agcgatcatc
     6841 gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga aatgccgctt
     6901 tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa tgaccatatc
     6961 aatatgccat gggtgatcct ctcttccagt gtcgacgaaa aactgttccc gcgtgccgta
     7021 cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt ctgggcatcg
     7081 gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg tttgcgcacc gaaattacaa
     7141 caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga ggataaaagg
     7201 aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa ccgaccgcat
     7261 ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag gcaataaagg
     7321 gcaaatcaaa gaagagatgg gcacccatct gtggatcaca gctcgtatgt tgcacgttta
     7381 ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg ttggttgatc acggtattaa
     7441 agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct gcgtgaatga
     7501 cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc tgctgggtgc
     7561 cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt acaccattga
     7621 gattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggagt cctgggacga
     7681 agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg cggtggaagc
     7741 tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg cgattcgcgt
     7801 ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta acgaacactt
     7861 cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc atcgcttccg
     7921 cgcgttcgga ggtacaccag gccactggat cgaatggggc cgtttaatgc tgcacatcca
     7981 tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag atgccaaagg
     8041 tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggagcggacg ggattgttta
     8101 taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc ctatcgtcga
     8161 agcaatgggt acggtctacg cgctctacac cgtcactggc gatcgccagt atgaaacctg
     8221 gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa atggttcctg
     8281 gtggcaggag ctggatgcgg acaataaggt catcaccaaa gtctgggacg gcaaacagga
     8341 tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc ccggcatggc
     8401 tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct gaaaataggg
     8461 tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccggtgca
     8521 gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa ggtgccttgt
     8581 atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc gggggattat
     8641 cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca gggttgtcat
     8701 ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa agtgcttatt
     8761 ccgtggccga accggatcgc caacggctgt taccgttatc aggggcagga atatcagtta
     8821 cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg gcgagactgg
     8881 caaattagtg aattaaccac caccagcgtt accctgacag cttttctgcc gcccagttac
     8941 ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca cacgggtctt
     9001 tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg cgtgggtatt
     9061 catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca gcttccggca
     9121 aaccaggttt atgccgtcga tgaacacgta aatccgacaa cgcttcacca cgttgatgag
     9181 ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga ccacaccttt
     9241 aaaaccgcta atgatttgtg ggaaatgacc attacccatc cacagcaggc gctttcggta
     9301 agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt acagcgtcag
     9361 ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg tatcgactta
     9421 ttattactgg aaccaggaaa accgcaccgc ctgtttttta atatttacgg acagagaaaa
     9481 taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct aaaggcaaca
     9541 ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt ttttatatat
     9601 cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac aatctcgttc
     9661 tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga ttttcaattt
     9721 catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt aacccataac
     9781 aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat gttccgcggt
     9841 aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc catcgtcagc
     9901 cagtcaccgg atggttggtt aattcatttc agccgtggtt ctgacattag cgccacgctg
     9961 aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga caaccttaac
    10021 cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta cggctgcggc
    10081 gaacagtttt cctacttcga tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa
    10141 ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa agaaaatgcg
    10201 ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac gcagaagtat
    10261 tactgccatg ttgataacag ttgctacatg aacttcgact ttagcgcccc ggaataccat
    10321 gaactggcgc tgtgggaaga caaagctacg ctacgttttg aatgtgctga cacatacatc
    10381 tccctgctgg aaaaattaac tgccctgctg ggacgccagc cagaactgcc cgactggatt
    10441 tatgacgggg taacgctcgg cattcagggc gggacggaag tgtgccagaa gaaactggac
    10501 accatgcgta acgcgggcgt gaaggttaac ggcatctggg cgcaggactg gtctgggatc
    10561 cgtataacct cttttggcaa gcgcgtgatg tggaactgga agtggaacag cgaaaactac
    10621 ccgcaactgg attcacgcat taagcagtgg tatcaggagg gcgtgcagtt cctggcctat
    10681 atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca acacggctat
    10741 ctagcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt ttacggcggc
    10801 gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat caaaaagaac
    10861 atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct gcccaccgac
    10921 acgtacctgc ataacggcgt cagtgccgaa attatgcata acgcctggcc tgcgctgtgg
    10981 gcgaagtgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat cctcttcttt
    11041 atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc gggcgaccag
    11101 aacgtggact ggagtctcga cgatggcctg gcgtccgtag tcccggcggc gctgtcgctg
    11161 gcaatgaccg gacatggtct gcatcacagc gacattggcg gttacaccac cctgtttgag
    11221 atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg
    11281 atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg cgatgcggaa
    11341 actatcgccc attttgcccg tatgactacc gttttcacca ccctgaaacc ttatctgaaa
    11401 gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca tgcgcccgct gttcctgcat
    11461 tacgaagacg atgcacatac ctattccctg aaatatcagt acctgttagg ccgcgacatt
    11521 ctggtcgctc cggtacacga agaaggtcgc agcgactgga cgctctatct gccggaggat
    11581 aactgggtac atgcctggac gggtgaagcg ttccggggcg gggaagttac cgttaatgcg
    11641 cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc actgttcgcg
    11701 tcgttaaaca gcatctaatc acatccgccc gtggggaaac tcatgggcaa agggagaaca
    11761 tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt taaagagaaa
    11821 ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg tacgctttat
    11881 cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg cattatcttt
    11941 ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat gttggattcc
    12001 cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc gtcattcccg
    12061 gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg
    12121 gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat gaactgctcc
    12181 tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg aacgcgcctc actggcggca
    12241 tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt cgtgccagtt
    12301 atgaatctta tcgaaggtaa tcagaaactt ggctatatct tcgccgccac gctgttttca
    12361 ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg ttatgtcgaa
    12421 acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg cgcaattgcc
    12481 ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg ggcgtttaac
    12541 gtcaagctcg ccattcaggt ctattacacc cagtacgtac ttaacgatcc catcctgttg
    12601 tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct gatgcctggc
    12661 gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg
    12721 ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc gttctcctgc
    12781 ctggcgttct tcggctcagc gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg
    12841 cgagtggcgc accggcgtgc gttcggaagg cacggtctac accggcttca ccttctttcg
    12901 caaagtgtct caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaattggcta
    12961 tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg ttacgccagt tgatcttcat
    13021 ctacccaagc gcactggcgg tagtcaccat tgtggcgatg ggttgcttct acagcctgaa
    13081 cgagaagatg tatgtccgca ttgtggaaga gatagaagcc cgtaaacgca cggcgtaata
    13141 ataataaacg acgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact
    13201 gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg tcggttcgaa
    13261 tttaatgctc tgcatcggta ctctgtatct cctcaaactt tataccgatg aattagggat
    13321 gcctgcttac tatggcggga ttatctttct ggtcgcgaag ttttttaccg cgtttaccga
    13381 tatgctcacc ggatttttac tcgactcgcg gaaaaatatt gggcctaaag gcaaatttcg
    13441 tccctttatt ttatatgcag cagttccggc ggcgttaatt gcaacgctcc agtttatcgc
    13501 caccaccttt tgtttgccgg ttaaaacgac gattgccacc acgctgttta tgatgtttgg
    13561 actttcatac agtctgatga actgctcgta tggtgcgatg atcccggcaa ttaccaaaaa
    13621 cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt
    13681 gatttgtacc gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta
    13741 tgcctgtgcg gcacttatgt tctccattgg cggctttatt tttatgatgc tgtgctacag
    13801 aggcgtcaaa gagcattatg tggacacagc gccaaccgga cataaagcca gtattctcaa
    13861 atctttttgc gcgatatttc gtaatccacc attgctggtt ttatgcattg ctaacctgtg
    13921 tactctggcg gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct
    13981 gaatgatatt aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg
    14041 cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg
    14101 catggtgcta tgggcggtgg gtgatatact gaattatttc tggggaagta actctttcac
    14161 tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc tttgtaaaca gcctgaactg
    14221 ggcactggta ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg
    14281 ttctgtttat accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt
    14341 gccaggcatt atgctgacgc aaattggtta tgttcccaac atcgcgcaaa gcgatgcgac
    14401 attacagggt ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc
    14461 attgacgatg ggattctttt acacactcaa tgaaaaacgg tttgcattga ttattgagga
    14521 aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa aaaacggctt ccgtaacgtt
    14581 ataaatgtca atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa
    14641 atggacttat tatgaaaaag attaatgcaa taattttatt atcttctctg acctctgcct
    14701 cggtatttgc tggcgcatac gtcgaaaaca gggaggcata taatcttgcc tccgatcagg
    14761 gcgaggttat gctacgtgtg ggttataact tcgatatggg cgcgggtatt atgttaacca
    14821 atacctacac ctttcagcga gaagatgaac taaaacatgg ctataatgaa attgaaggtt
    14881 ggtatccttt attcaaacca accgataaat taaccatcca gcccggtggc ttaattaatg
    14941 ataagagtat cagttcgggt ggtgcagtgt atctggac
//
LOCUS       DAJTRM010000094        14461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRM010000094 DAJTRM010000000
VERSION     DAJTRM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.094"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(105..371)
                     /locus_tag="OO322_003177"
     CDS             complement(105..371)
                     /locus_tag="OO322_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5907959.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            645..4169
                     /gene="nifJ"
                     /locus_tag="OO322_003178"
     CDS             645..4169
                     /gene="nifJ"
                     /locus_tag="OO322_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5907960.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4536..5665
                     /gene="ompN"
                     /locus_tag="OO322_003179"
                     /pseudo
     CDS             4536..5665
                     /gene="ompN"
                     /locus_tag="OO322_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5806..6240
                     /gene="uspF"
                     /locus_tag="OO322_003180"
     CDS             5806..6240
                     /gene="uspF"
                     /locus_tag="OO322_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5907961.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6416..7351
                     /gene="ttcA"
                     /locus_tag="OO322_003181"
     CDS             6416..7351
                     /gene="ttcA"
                     /locus_tag="OO322_003181"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5907962.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7480..8847)
                     /gene="dbpA"
                     /locus_tag="OO322_003182"
     CDS             complement(7480..8847)
                     /gene="dbpA"
                     /locus_tag="OO322_003182"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5907963.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9325..10308)
                     /gene="zntB"
                     /locus_tag="OO322_003183"
     CDS             complement(9325..10308)
                     /gene="zntB"
                     /locus_tag="OO322_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5907964.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10563..11795
                     /gene="dgcM"
                     /locus_tag="OO322_003184"
                     /pseudo
     CDS             10563..11795
                     /gene="dgcM"
                     /locus_tag="OO322_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11816..12379)
                     /gene="smrA"
                     /locus_tag="OO322_003185"
                     /pseudo
     CDS             complement(11816..12379)
                     /gene="smrA"
                     /locus_tag="OO322_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12633..14087)
                     /locus_tag="OO322_003186"
     CDS             complement(12633..14087)
                     /locus_tag="OO322_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5907965.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14084..>14461)
                     /locus_tag="OO322_003187"
     CDS             complement(14084..>14461)
                     /locus_tag="OO322_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5907966.1"
                     /translation="DGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGM
                     KPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAF
                     DDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3854 a         3645 c         3415 g         3547 t
ORIGIN      
        1 agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg
       61 accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag
      121 ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca
      181 atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt
      241 gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc cgcagtcgcc
      301 tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc tacccaaaac
      361 gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag
      421 gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt
      481 atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg tgggggacac
      541 aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc
      601 agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg
      661 gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc atctacccta
      721 ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga
      781 acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta
      841 ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt
      901 tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca tttgtcctgc
      961 atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat cattccgacg
     1021 ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc caggaagcgc
     1081 aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca tttattcatt
     1141 tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca
     1201 ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc
     1261 cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg
     1321 aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg atgaatgatt
     1381 tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat ccgcaagcgg
     1441 aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat
     1501 tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt cccttctccg
     1561 ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa
     1621 ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag
     1681 aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg cgctatggtc
     1741 tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg
     1801 ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat ctgtcactgc
     1861 cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt tatggccttg
     1921 gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt aattccacgc
     1981 cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc ctgacggttt
     2041 ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt
     2101 ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac
     2161 ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc
     2221 cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg
     2281 cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt
     2341 ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga
     2401 ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg caggctctgg
     2461 cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca
     2521 atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcggcga
     2581 tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc
     2641 cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag
     2701 aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca gctattcgcg
     2761 caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctagatg
     2821 tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca
     2881 ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta
     2941 aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc aattacgatt
     3001 tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat
     3061 cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt ggcgagacgc
     3121 cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct aacgccaccg
     3181 gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg
     3241 gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt
     3301 tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg
     3361 ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc
     3421 gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaacttc
     3481 tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc gacggctggg
     3541 cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca
     3601 ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac
     3661 cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg
     3721 gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc
     3781 tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca tcgctgatca
     3841 ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac gaccagatgc
     3901 gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt cgtgccgatg
     3961 aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt
     4021 tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt
     4081 tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca caaatggccg
     4141 gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa
     4201 aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat
     4261 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc gcgcagaata
     4321 acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg atacattcac
     4381 cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag gcgtaacaaa
     4441 tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc gaaatgggga
     4501 taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta ctggcacttt
     4561 taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat aataaagacg
     4621 gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct gataattcag
     4681 cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc caaattaacg
     4741 atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac actgaatctt
     4801 caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca gattacggtt
     4861 ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg accgatatgc
     4921 tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact ggtcgagcca
     4981 atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt ctgaacttcg
     5041 cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc accaataacg
     5101 aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca tatgatttag
     5161 gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat gaccaggtta
     5221 accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta aaatacgatg
     5281 ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc ccgtttggcg
     5341 acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt accagtttga
     5401 tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc ttcacgctgc
     5461 tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt
     5521 tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata aaatcaacct
     5581 gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg atattgtcgc
     5641 tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg
     5701 cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg
     5761 tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt
     5821 cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag ccacgttgag
     5881 gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc ttcactgccc
     5941 tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa
     6001 gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga
     6061 gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagaag
     6121 atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc
     6181 ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga
     6241 cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt
     6301 tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt atgctgagcg
     6361 ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc
     6421 gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac aaaaacgtct
     6481 gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag gcgatcgcat
     6541 catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc tgcgcaattt
     6601 gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc aaaagcaacc
     6661 gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg agtacaagat
     6721 tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca aaaccacttg
     6781 ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg aactggggac
     6841 gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt tcttaaatat
     6901 gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg atggcaaaca
     6961 tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat ttgccgatgc
     7021 aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca
     7081 ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta tcgagacgat
     7141 gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc tgttcgattt
     7201 caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt ttgatcgcga
     7261 agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc agttggatga
     7321 gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc gcaagcgtat
     7381 cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg caatcccgaa
     7441 taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata accgcacccg
     7501 gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat gagcaacagc
     7561 ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa tatctgcgcc
     7621 atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca ttttggcttt
     7681 tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa tggaactatt
     7741 agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga tattggcccg
     7801 ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg cacgagctgt
     7861 acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt tcaccaccag
     7921 ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca gtacacgggc
     7981 gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc cgtgtaacga
     8041 caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat cttttttggt
     8101 attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt gcaacagagg
     8161 aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca aagcatctgt
     8221 tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg cggcgatggc
     8281 ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa aacggatgac
     8341 atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat ccatcaccaa
     8401 agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca aacgacccgg
     8461 cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac cgaacggttg
     8521 accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc
     8581 acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct gggtttgaaa
     8641 tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc
     8701 ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg cggcggcctg
     8761 caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg cgggaggcaa
     8821 aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac gcaaagttag
     8881 caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg ttcttcatcg
     8941 ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg aacaggatcg
     9001 ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat atttccctcc
     9061 ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa aaaaagccga
     9121 ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt
     9181 aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca agggagatat
     9241 tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt ttttgtatac
     9301 agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc ataaagcaac
     9361 accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt gccacccgcc
     9421 gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac tgggtaaaaa
     9481 gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct gcatcacctg
     9541 agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt cgtcaaggcc
     9601 gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg
     9661 caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat agcgacgcat
     9721 cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt gctgatcaag
     9781 gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt cactggaatg
     9841 atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg gacccgtgcc
     9901 ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc gggtcgaaac
     9961 aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc gttcatcggt
    10021 gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc tgactcgggg
    10081 ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc
    10141 cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg gatgcgcttc
    10201 atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca gcatccaggc
    10261 aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg tgcctccatc
    10321 atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc tcatcacccc
    10381 attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt
    10441 tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa ttatctgatc
    10501 atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat aacgggatct
    10561 caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc tggattgttt
    10621 cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg atgcaagacc
    10681 tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa gaactgccga
    10741 aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact gttcagcgta
    10801 aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca gaaacagaac
    10861 cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa gccagtcgct
    10921 cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg actaactctg
    10981 caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct aacctcgccg
    11041 cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc taggaaataa
    11101 atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta cccggcggtc
    11161 ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat gtgcagacct
    11221 atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg catgatatta
    11281 ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac gcgatgaccg
    11341 gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg cagcatctcg
    11401 ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa cacattaacg
    11461 atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc cccctcgaaa
    11521 gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt gtcttattgc
    11581 taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc gtaagcgtgg
    11641 caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg gcgcatcacg
    11701 aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg tatcgggcga
    11761 aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct cgagatcaac
    11821 gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt ttacgtagtg
    11881 cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat gcctgaacat
    11941 catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca tgtgatttat
    12001 cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc agggcttgtt
    12061 gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa agattcaggc
    12121 tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc tacaaccctt
    12181 cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg gtagtgagga
    12241 aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc gttggatgcc
    12301 agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca agaaacagcg
    12361 atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga ttcagacggg
    12421 ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac tctacttcat
    12481 acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag tatcgaacag
    12541 tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca tagcaggaac
    12601 caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga aaatgccata
    12661 gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag tatttccgat
    12721 gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa gtcattcacc
    12781 gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt agttcgatta
    12841 ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg tgttaaatta
    12901 aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt taatgttacg
    12961 atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa cagtaattcc
    13021 tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac aaatccgccc
    13081 agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa gtaaaaggga
    13141 acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc ttcggcattt
    13201 aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa tatcccaacc
    13261 cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa agcatgaaga
    13321 tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa actcaccaat
    13381 gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg cgctccttct
    13441 tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact cggataatca
    13501 gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg aatactcaaa
    13561 actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc gaggttatca
    13621 gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag cgcactgact
    13681 accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat cgtgaaaact
    13741 aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat catgatctga
    13801 atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc ggatagagta
    13861 aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat aacaataatc
    13921 tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc ggacagacca
    13981 ccacctattt catttaggga tgacgataca atttgaatat caagctgagc aataaataat
    14041 cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc tcatcaacac
    14101 tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg tcgtcaaatg
    14161 caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca ttttctatcg
    14221 gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga ccgtgaaaca
    14281 cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc attcccgcta
    14341 tttggttttc tttataattt gcatcaaccc acaactcact gactggtact agtgaaacta
    14401 acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg acaatgccat
    14461 c
//
LOCUS       DAJTRM010000095        14274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRM010000095 DAJTRM010000000
VERSION     DAJTRM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.095"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            170..313
                     /locus_tag="OO322_003188"
     CDS             170..313
                     /locus_tag="OO322_003188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907967.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(532..2070)
                     /gene="aldB"
                     /locus_tag="OO322_003189"
     CDS             complement(532..2070)
                     /gene="aldB"
                     /locus_tag="OO322_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5907968.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2235..3386)
                     /gene="yiaY"
                     /locus_tag="OO322_003190"
     CDS             complement(2235..3386)
                     /gene="yiaY"
                     /locus_tag="OO322_003190"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5907969.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3576..5420)
                     /gene="selB"
                     /locus_tag="OO322_003191"
     CDS             complement(3576..5420)
                     /gene="selB"
                     /locus_tag="OO322_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5907970.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5417..6808)
                     /gene="selA"
                     /locus_tag="OO322_003192"
     CDS             complement(5417..6808)
                     /gene="selA"
                     /locus_tag="OO322_003192"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5907971.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6906..7514)
                     /locus_tag="OO322_003193"
     CDS             complement(6906..7514)
                     /locus_tag="OO322_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5907972.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7585..8670)
                     /locus_tag="OO322_003194"
     CDS             complement(7585..8670)
                     /locus_tag="OO322_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5907973.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8673..9035)
                     /locus_tag="OO322_003195"
     CDS             complement(8673..9035)
                     /locus_tag="OO322_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5907974.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9572..11485
                     /gene="mtlA"
                     /locus_tag="OO322_003196"
     CDS             9572..11485
                     /gene="mtlA"
                     /locus_tag="OO322_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5907975.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11580..12728
                     /gene="mtlD"
                     /locus_tag="OO322_003197"
     CDS             11580..12728
                     /gene="mtlD"
                     /locus_tag="OO322_003197"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5907976.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12728..13315
                     /gene="mtlR"
                     /locus_tag="OO322_003198"
     CDS             12728..13315
                     /gene="mtlR"
                     /locus_tag="OO322_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5907977.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13327..13536)
                     /locus_tag="OO322_003199"
     CDS             complement(13327..13536)
                     /locus_tag="OO322_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907978.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13820..14182
                     /locus_tag="OO322_003200"
     CDS             13820..14182
                     /locus_tag="OO322_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5907979.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3466 a         3813 c         3598 g         3397 t
ORIGIN      
        1 aatataaaaa agaacaatta acgcatattg tgaaatgcca tgatgcagtg caatttcctt
       61 atttatataa cgacatattg ttttttatat actttctgaa atgtgctgtc tggtttttca
      121 acaggacgag gtttatcata ttgaaatagt ggaactattg gccaaactaa tgaataactc
      181 cagtaaaaca tccacagtac agattaagcg tattaaacct tcaattatct accgtttatt
      241 gctgattggc ctcggatcac caatggtgat ttacggcctg gttcgcccgc tcaccatcga
      301 aacgcgagat taaactcctg acaaaacacg ccccagaaac gcacttctgt tggcgtagtc
      361 tgggttattg cgcacgtagg tttaaaacgt aaattgtaac aacgtgcgct ttgtttatgc
      421 cggatgcggc gtgtacgcct tatccggcct acggtcaggt tcccgtaggc atgataagac
      481 gcgtaagcgt cgcatcaggc aatgaatacc caatgcgacc agcttcttat atcagaacag
      541 ccccaacggt ttatccgagt agctcaccag caggcacttg gtttgctggt aatgctccag
      601 catcatcttg tgggtttcgc gaccgatacc tgattgtttg tagccaccaa acgcagcatg
      661 tgccgggtaa gcgtgataac agttggtcca cacgcgccca gcctgtatgc cgcgccccat
      721 cttataggcc agattaccgt tgcggctcca gacgcccgcg cccaggccat attgcgtatc
      781 gttcgccagc tccagcgctt cttccatcgt tttgaaggtg gtcaccgcca gcaccgggcc
      841 aaaaatctcc tcctggaaca cccgcatatt gttctgacca aacagaatcg tcggttcgag
      901 gtagtagccg tctttcagtt caccttccag cagcttgcgc cgcccgcctg tgagcacgtc
      961 agcgccctct tttttaccga tatcaatgta gttgaggatg gtttccagtt gcccgtgaga
     1021 aacctgcgcg cccatttgcg tcacgctgtc gagcgggtta ccgctgcgaa tgctttcgac
     1081 acggcggatg gcgcgttcca taaagcgttc gtagattgac tcctgcacta aggcacgact
     1141 cggacaggtg caaacttcgc cctggttaaa ggcaaacagt gcaaagcctt ccagcgcttt
     1201 atcgaaaaag gcatcttctt catccatcac atcagcaaag aagatatttg gcgacttacc
     1261 gcccaactcc agcgtcaccg gaataatgtt ttgcgttgcg tattgcataa tttgttggcc
     1321 cacttccgtt gagccggtaa acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc
     1381 gccaatttcc ccacctgcgc cattgaccac gttcaccacg cccggcggca gtaaatcacc
     1441 gacaatttcc attagcagca gtacagaaag cggggtaaga cgtgcgggtt tcagcaccac
     1501 acagttgccc gccgccagcg cgggagccat tttccagctc gccatcagca gcgggaagtt
     1561 ccacgggata atctgcccca ccacgcctaa cggttcatgg aaatgatagg ccacggtttc
     1621 gctatcaact tcactgatcc caccttcctg cgcccgaata cacgaggcga aatagcggaa
     1681 atggtcaatc gccagcggta catccgcagc actggtttcg cgaatgggtt tgccgttatc
     1741 ccaggtttca gctgtcgcta acagctcgag gttttgttcc attcgatcgg caatcttaaa
     1801 cagaatcgcc gcgcgatcct gcaccgaggt gtgcgcccat ttatctttca ctttgtgcgc
     1861 agcatccagc gccagatcga tgtctcgttt gcccgaagac gccacttcgc acagcagctg
     1921 cccggtcacc ggcgtcagat tctggtaata ctcaccatcg gcaggggcta cccattcacc
     1981 gccaataaag ttgtcgtagc gggcttttaa cttgatgggg aaaccatact cgccgggctt
     2041 aatctgtgct gaagggggat tattggtcat gatcgtctcc ttgcggtgtg aggtataaca
     2101 agggtagacg tgactggcga aatcttcgcc agtcggtaac agctttacga cagctatcac
     2161 gaatttacgg gcaaggatta cagagatagt tgaaaaagcg gctgacaatt tgccagccgt
     2221 tgttggaatg atgattacat tgcggcgcga taaatcacca caatttcttc gtgagttgcc
     2281 tggatcgggt tagtaaagcc acaggcatct ttcagggcat tagtcgccag aaccgcgaaa
     2341 tcttcttctt tcacgttcag gtcgcgtagg cctgccggga tatccacttt cttcgccagt
     2401 tcacggatgg cgttaatgca ggcttcagca ccttccgcgt cgtttttacc tgtcacgttc
     2461 acgcccattg cagcggcaca gtcacgcaga cgtgcagcgg cgactttgct gttgaatacc
     2521 tgaacatgcg gcagcagaac ggcgttacat acaccgtgtg gcaggttgta gaaaccgccc
     2581 agctggtgcg ccatcgcatg aacataaccc agagaagcat tattgaacgc cataccggca
     2641 aggaactggg cataagccat tgcttcacgc gctttcgcat tactgccatc ttcaacggct
     2701 aacggcaggt tttcggcaat catggtcacg gctttcagtg cacaagcgtc agtgatcggc
     2761 gtggcggcaa tagaaacata cgcttcgata gcgtgcgtta aggcatccat acccgttgcg
     2821 gcggtcagtg acttcggcat accaatcatc agagaggagt cattgacaga aagcagcgga
     2881 gtcacgtgct tatcaacaat cgccattttg ttatgacgcg cttcgtcagt gatgatgcag
     2941 aaacgggtca tttcagaggc cgtacccgcc gtggtattga tggcgatcat cggcagctgc
     3001 ggttttgcag agcggtcaac gccttcgtaa tcgcgaatat cgccgccatt ggctgccacc
     3061 agcgcaatac cttttgcgca gtcgtgtgga gaaccaccgc ctaaggagat cacgctatcg
     3121 caattatttt ctttaagtaa tttcaaacct gcggcgacgt tttccgtggt ggggttaggt
     3181 tgggtgccat cataaataac gctaaaaata ttgcgttctt ccagtgcttt ttgcacatca
     3241 cccgccatac ctaatttcgt taacatattg tcagtgacaa ttaaggtacg ggtaaatcca
     3301 taatctgcca tcatattcat tgcatcagtc aatgaatcag cgccgatgac attcacagaa
     3361 ggaataaaga aagttgaaga tgccataaca cactcctgaa agtgtaaaaa agggtgggca
     3421 gcttactgag gattttcatt cgaaaacatg atcattatta ttaattatca aaattaatct
     3481 aataaaaagc acattgtatt cattaaacaa tgtgctttat ttaaaatcat aagaaatttg
     3541 tattaattta ttttaaaaat taattaatca tttccttatt tttccggaaa taataatgcg
     3601 tcgcgcaata aatgatcatt tccacgacga cgcgtaaagc caatgcggtc aaaatattcc
     3661 agaatctgaa ttgccagctt tcggcctacg cctaagcgat cgcggaaatc cgccgcgcag
     3721 gttgaaccac actcctgatc gagatcgcgg atcatattgg caaattcgac aatccgatcg
     3781 ttacggtaat aacgatcttt gacgatcgcg gtaattattc cttgctgcgc cgcctggcgt
     3841 agcgtcaggc gcattgcctg ctcgtcggta cccgtctctt ttgccagatc acgcacccac
     3901 cacggctcgt caccaaacag cggctctgct ttttgccaga tggcctgctg ctcttcgctg
     3961 aagcccgctt tgtgatccgg cagatgcagc caaccgtgat gactgaggat gtcgccgctt
     4021 tcgcgcatct tttcaatcag caacagtact agcgcttcat cttccattgg caacgccata
     4081 cgtcgcagac gttcgcgccc aggcccaggt tcatcgcgat gttgctcatg ataagtcgct
     4141 aatgtgtcga gaattttccg ttgccagcga gcggcaaccg gcgcattcaa caagctataa
     4201 ccagcctgaa tatagccagg ctgttgcagc agttcgcgca tcccttcgcc gttgagctgg
     4261 cgcgcccaag cgaaatcagg aagattaacc gcgccgcgtt gcaggtgaac ggctaacgct
     4321 tcgctatcat tctgtgccct ggcgagggaa gccagccatt gcagatactc tggtttacgt
     4381 ttaccgcgac gcggcgggtt aagcatcacg acgcgcgctc cggccagcgt gttgcgggca
     4441 gagatatcgc gcaataccag gcggtcgtta tctgccagcc ataacggggt gtcgaagacc
     4501 agttcagcaa ggttatcttc cagcagtgaa acgcgtcccg tgacgtggct ggcggcgtgg
     4561 tgaatatgca gcggctgcca ctgggtcagc ggtgcatggg tttgaagctc gacaatcacc
     4621 cgtgtgaacg gctctggcgg cacatcggca agcagccagt cgccacggtt aatctgctct
     4681 ttttccgcat cacccgcgat gtcaagcgcg atacgctgcc cggcattggc ggtttctgtt
     4741 ggctggtttt gcgcatgcag cgcacgtaca cgcatcggtt tatttacacc agtcagccag
     4801 agtgaatcgc ctaccttcac ttccccgctt aacgccgtac cggtgacgac cagcccggca
     4861 ccttttacgg taaatgcgcg gtcaatcgcg aggcggaaac tatgttggct ggcgtgctcg
     4921 cgttccggca actgaagcag atgctcgcgc agggcatcaa ttccccgacc ttcggttgct
     4981 gcggtgataa acagttttgc ctcagcaaaa ccgtattccc gcagaacctc ctttacctgg
     5041 cgttcaacct catcaacacg cgcttcgtcc acgcgatcgg ctttggtcag cgccactgtc
     5101 agcatcgggt taccggtcag ctgcaaaatc gccagatgct cacgggtctg tgccatcacg
     5161 ccgtcatcgc acgccaccac caacagcgcg tgatcgatac caccaacgcc cgccagcatg
     5221 ttggaaagaa acttttcatg accgggaacg tcgataaaac caggcacgcg accatccggc
     5281 tgcggccagt aggcatagcc gagatcgatg gtcatgccgc gctttttttc ttccggcaga
     5341 cggtcagcat ttacgccagt aatcgcctgc aataaggttg ttttgccgtg gtcaacgtgt
     5401 ccggcagtcg caataatcat ttcaacaaca tctccaaaaa ccgttgctca tcttcaaggc
     5461 agcgtaaatc cagccacaat cgtccgtcat aaatacgacc aatcaccggc actggcaatt
     5521 cacgccagcg ggcggctaat gactcaaggt ggctaccgcg tccatcatgg ggtgtaaacg
     5581 ttaatgccgc gctcggcagg cgatcaaccg gcagcgaacc actgccaatc tgcgaaagac
     5641 atggcataac ctgtaccgca aactccgcgc cgtaatgtgc gacaagggga gcctgtaaac
     5701 gttgtgcctg gatttgaatg acctctgcgc tgcgggtaag caggcgcagg gtcggtaatt
     5761 tttcactcag agcttcaggg tgtaaataaa gacgcaacgt ggcttccagc gccgcgaggg
     5821 tcattttatc cgcgcgtaat gcacgcttca gcgggtggct ttgcaggcgg gcgatcatct
     5881 cttttttacc aacgataatc ccggcctgcg gcccgcctaa caacttgtcg ccggagaagc
     5941 tcaccagact gacgcccgcc gcaatcaact cctgcggcat tggctctttc ggcaaaccgt
     6001 actggctaag atcgaccagc gagccactgc ctaaatcagt caccactgga acatccagct
     6061 ctttgccgag cgccaccagt tcagcttcgt ctatcgcttt ggtgaacccc tgaatgctgt
     6121 agttactggt atgtactttc atcaacagtg cggtattttc attcaccgcc tgacgataat
     6181 cattcgcgtg cgtgcggttg gtggtcccta cttcgtgtag ggtgcagcct gcctgacgca
     6241 taacatcggg aatacgaaac gcgccgccaa tctccaccag ttcgccgcga gataccacca
     6301 cctctttccc gctggcagtg gccgccaaca tcaataacac cgccgccgca ttgttattga
     6361 cgatacaggc atcttccgcc cccgtaatac ggcacagcag ctgcgccagc gcccgatcgc
     6421 gatgtccgcg tccggcgtcg tccagatcgt actcgagggt cactggcgaa cgcatagcct
     6481 gcgcaacggc ttccaccgcg gcttccgcct gtaaagctcg cccaaggttg gtatgcagca
     6541 cggttcccgt caggttgatc accggacgca gcgcgctctg cgcctctttc gtcaaccggg
     6601 catcgacttc ttgcgcccag ttttcacacc acgcaggcag cgtctggctg ccacgaatca
     6661 cttctcgcgc ttcgtcgagc atctgacgca acaattccac cacgcgggtg tgaccataag
     6721 tatcacgcaa agaaaggaag gagctatcgc gcaataagcg atcaatagcc ggaagttgac
     6781 tatagaggga acgcgtttcg gttgtcatag gaaacctggc tgatcaaggc cctctcacac
     6841 ggagaagggc gtttaacata accacggatt gtaacgtgag atgggtcagg aggacatatc
     6901 gcgcatcaag cctttggcgg ttcggtgcga gcaaaacttt cgcggctaaa caggttttcg
     6961 accagtttga ctaagtgtgg gcggtcgaca caccaacctg gcgcaacgcg gcggaaattg
     7021 agatatccga cagcacaggc aatggcgata gtcgccagat tgaccgtatc ggttttgagt
     7081 gtgccatcga ccagatatcc ttccagcaca tccagactgc ggttgatttt ctcccgctgg
     7141 cgtaacaatt catcttcaga ctgctgcgcc gccggacgcg cctgttcacg caccgatacc
     7201 agcccggcat ccataatgcc atccgccagc gcctcaattt tgcgcacccg caacgactcc
     7261 agcggatcgc gcggcaacat cgccggagcg acattcatta actcaatata ttcagcgatg
     7321 atcggcgaat caaaccagca ttcgccctct tcggtcacca gcaccggcac tttccctaac
     7381 gggttaaatt gcgccacgcc gttatccgcg ttatagggca gttcattaat aaattcgaaa
     7441 gttatgccct tttctaacaa cagaataaaa agtttgcgta caaacgggct ggtgtagcta
     7501 ccgacgagtt tcatgccgag tcctttgtgc gaggaaaaat atcagtatgg ctcaggctca
     7561 cgcaaaacac aggtgatata agtctcaatg atccaaataa agataatgca tccaactggt
     7621 cattcttagc agcactttcc gcatcaccga aacatggctg aaatggtcgg agtaaaccgc
     7681 cacctgggcg tagatgccgt cgggtaaggc atcgatatca tcgttagggt ccagttcaat
     7741 ggttcccagc acaccgtccg tgccgggaac gaccgttaat gattgcaata ccccctgcgc
     7801 ctgataagaa ccgcctggca cgacaggtaa aatactagtc aatttgccgt ggaacacctg
     7861 cccaggtagc gcgttaaaca ccacttccgc atcatcgcca ggtttcagac gtaacagcga
     7921 gttttgccga aattgggcga caatttgccg tttttgctcg gggatgaaga ccatcaccgg
     7981 acgcagcggc aaggcagctg cgtatgtacc tgggcggatc agtacctgag tgacgtagcc
     8041 attgcttggt gcgcgaatga cagtctgctc aagattatat tttgcttcag taagttgcgc
     8101 tcttaagctc acaatctgcg attgctcgcc gttgaccata ctgtcgagct gactctggat
     8161 ctgcgcctgc tccgccaccg agcctttcac taacgcatcc tgcgcgagga aattttgccg
     8221 cgcatcgtcg atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc ctttcaggta
     8281 acgttgataa tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg
     8341 cgcttcagtg agctgcgcac gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc
     8401 ctgaagtctg tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc
     8461 cttttgaata agctgattat tcttgtcagt gacttcagta acaattcccg tcacctgtgg
     8521 cgtgataggg atcgctatca ctgccttttg cgcggtaaaa gtgtaagggt ggttgtagtt
     8581 catcaacaaa atcaaaccac tcaccagaaa cacgcctccc agcgccgccg tcgccagcgc
     8641 cacgtaagtt aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca
     8701 gtctttttct cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg
     8761 atttccggtt cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc
     8821 tccggttgat agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac
     8881 aggctcaccc agcccgccgt atgaatagcg tcggcatggg ggtgattgcg ctttttggca
     8941 atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag
     9001 atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca
     9061 gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg
     9121 gttcgcgatt ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt
     9181 acataacgat tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta
     9241 agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga
     9301 cattactcac atttaaatgc catttttagc gaaaatcacc gccttgtttc ttttttatac
     9361 aggcgttttg tgatgagcgt cacgtcaatt acctctctac cccctatatt tatgtgactg
     9421 atatcacaca aaagcccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta
     9481 tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg
     9541 cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca
     9601 aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg
     9661 gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa
     9721 gctggtcggg ccgatgatca cttatctcct accgctgctg atcggttata ccggtggtaa
     9781 gctggtaggc ggcaaacgtg gtggcgtagt cggtgccatt accaccatgg gcgttatcgt
     9841 cggcgcagac atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg
     9901 cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt
     9961 gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat
    10021 tggcccgatt gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt
    10081 ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa
    10141 caacgccatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg
    10201 taaatcaatc ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc
    10261 gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca
    10321 cttcctgggt ggtatccacg aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat
    10381 cctcgcagtc atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg
    10441 tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg
    10501 tgcttatttc gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc
    10561 tgctattttg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg
    10621 tcgtatgcag gacatgaaag ctgagtctaa aggcacatct ccgctatctg cgggcgatgt
    10681 gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc
    10741 aagtgcgatg ggcgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcgcagat
    10801 ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctcg tcatcactca
    10861 ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag gcacagcata tttcgctgac
    10921 caacttcctc gacagcggcc tgtacaccag cctaaccgaa cgtctcgttg ctgcccagcg
    10981 ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag
    11041 tgctaacctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cagcaaccaa
    11101 agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgagccgga
    11161 atacgttcag gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat
    11221 cgcggtgcca cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt
    11281 ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt
    11341 gattggtatt gcagcccgta acaacgagca cattcaggtt atcaccagcc tgaccaatgc
    11401 actggatgat gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct
    11461 ggaactgctg gcaggtcgta agtaatccaa tcctaccctc tcctcacgga gagggtttgg
    11521 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
    11581 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
    11641 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
    11701 atgcccgtca tagctatcag gttcatgtgg tcggtgaaac cgagcaggta gataccgttt
    11761 ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg
    11821 atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca
    11881 aagggctggt gaaacgcaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg
    11941 aaaacatggt acgcggcacc acgcagctga aaggtcatgt gatgaacgcc ctgccggaag
    12001 acgccaaagc gtgggtagaa gagcacgttg gctttgtcga ttccgccgtt gaccgcatcg
    12061 taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg
    12121 aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca ggcatggagt
    12181 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
    12241 ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac gctattctcg
    12301 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca
    12361 agcgctacgg ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt
    12421 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac
    12481 tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatac agccttccgc
    12541 acaagaatct gattcagggg attgctggtg caatgcactt ccgcagtgaa gatgatccac
    12601 aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga
    12661 tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa
    12721 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
    12781 gcaggcaaca atggaacaaa cccaggccgt tgaaaaccgt gtgcttgagc gtctgaatgc
    12841 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa
    12901 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc
    12961 gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
    13021 gttgggcgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg cattgcgtga
    13081 agagctaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc ttggaccctt
    13141 tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
    13201 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
    13261 ttcactgact gagctgattt ccaaaatcag cttaaaaaaa gcctttcaaa agtaagcaac
    13321 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg
    13381 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
    13441 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
    13501 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
    13561 gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa
    13621 aacgtgctga atcctcaata atcatccgct gctgtttatt atctttttct atagatttct
    13681 taattaatca acgaattgcg tagtttttga gcatgttaat gattacgttg cagaactatt
    13741 acaatgtcat cagttgtgaa caaagcacct ggtcgcgcat actaggggct ataaatttat
    13801 ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac gaaatcaagc gtctcagcga
    13861 tcgcctggac gccatccgcc accagcaggc cgatctgtcg ctggttgaag ccgcagacaa
    13921 atatgccgag ctggaaaaag agaaagccac gctggaagca gaaattgctc gcctgcgtga
    13981 ggttcatagc caaaaactga gtaaagaagc acagaagctg atgaagatgc cgttccagcg
    14041 cgcaattacc aaaaaagagc aggctgatat gggcaagctg aagaaaagtg ttcgcggact
    14101 ggtggttgtg cacccaatga ccgcgctggg gcgcgaaatg ggcctggaag agatgactgg
    14161 attttctaag actacgtttt aagcccctca gacgactggt tcgatcgtat caacccgcat
    14221 ctggcttgcc ttagctggtc aatgcgggga ctctcctgtc tactcgccta atta
//
LOCUS       DAJTRM010000096        14243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRM010000096 DAJTRM010000000
VERSION     DAJTRM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.096"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..106)
                     /locus_tag="OO322_003201"
     CDS             complement(<1..106)
                     /locus_tag="OO322_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5907980.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO322_003202"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO322_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5907981.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="OO322_003203"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="OO322_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5907982.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="OO322_003204"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="OO322_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5907983.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="OO322_003205"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="OO322_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5907984.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="OO322_003206"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="OO322_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="OO322_003207"
     CDS             complement(3707..4198)
                     /locus_tag="OO322_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5907985.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO322_003208"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO322_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5907986.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO322_003209"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO322_003209"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5907987.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO322_003210"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO322_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="OO322_003211"
     tRNA            6843..6930
                     /locus_tag="OO322_003211"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="OO322_003212"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="OO322_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..8647)
                     /locus_tag="OO322_003213"
     CDS             complement(7643..8647)
                     /locus_tag="OO322_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5907988.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8719..9279)
                     /locus_tag="OO322_003214"
                     /pseudo
     CDS             complement(8719..9279)
                     /locus_tag="OO322_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9364..9561)
                     /locus_tag="OO322_003215"
     CDS             complement(9364..9561)
                     /locus_tag="OO322_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907989.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9573..10064)
                     /locus_tag="OO322_003216"
     CDS             complement(9573..10064)
                     /locus_tag="OO322_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907990.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10061..10435)
                     /locus_tag="OO322_003217"
     CDS             complement(10061..10435)
                     /locus_tag="OO322_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5907991.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10482..10856)
                     /locus_tag="OO322_003218"
     CDS             complement(10482..10856)
                     /locus_tag="OO322_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5907992.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11019..11240)
                     /locus_tag="OO322_003219"
     CDS             complement(11019..11240)
                     /locus_tag="OO322_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5907993.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11303..11779)
                     /locus_tag="OO322_003220"
     CDS             complement(11303..11779)
                     /locus_tag="OO322_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5907994.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11795..12268)
                     /locus_tag="OO322_003221"
     CDS             complement(11795..12268)
                     /locus_tag="OO322_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5907995.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12610..13431)
                     /locus_tag="OO322_003222"
     CDS             complement(12610..13431)
                     /locus_tag="OO322_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5907996.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13531..13704)
                     /locus_tag="OO322_003223"
     CDS             complement(13531..13704)
                     /locus_tag="OO322_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5907997.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13824..>14243)
                     /locus_tag="OO322_003224"
     CDS             complement(13824..>14243)
                     /locus_tag="OO322_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907998.1"
                     /translation="TNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAIDKA
                     LNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNPKTH
                     KVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3640 a         3491 c         3409 g         3703 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaactgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagcc tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact ccgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtagcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat actgccgggg aacaagtcct gtggaagctg
     7921 caaaatccct gctgcatgag aactgtttcc cgtcaccaag ttgcgacgat agtaagctgg
     7981 cagtaatcgg accaacacca ggaattgtca taatgcgctg agcggtatca tcagcgttga
     8041 ttgactgact taattctgat tccagctctg ctatttgctc aacaaggtaa aagtagtggg
     8101 tatgcaacct caccaaaagg cggctcagat attcggggat ttcgtgttca gccaacacga
     8161 gagacaaccg ttttattaca gcatcaccta tcggcaggct gattccaaat tccagcaaga
     8221 aaccgtggat ctggttggtt gttttcacct tatctctgat gagcgattct ctgacccggt
     8281 gtaacgccct catggcctgc tgggtctcag ttcttggctg gacaaaacgc attgatggtc
     8341 gtgaagccgc ttcgcatatg gcttctgcat caacaaaatc attcttatta cttttgacaa
     8401 acgggcgaac gaactgtgga gaaatcagct tagtttcatg cccaaaatca gagatacagc
     8461 gagccataaa gtgtgcaccc gcacaggctt ccattacaac aactgagggc ggacatgtcg
     8521 ccagaaactg catcagttta gtacgggtaa attttttacg cagaagagcc ttaccagact
     8581 tgtcttgaca atgaatgtgg aaggagtgct ttccgagatc gataccaata agtgtgatgt
     8641 tctgcatgat ggtctctccg ttaaagggat ttaccttaac agcgtactgc tgttaaggtg
     8701 ggggtgacca tatcattatc cccaccggtc ccatctcacc agattgttga cgataatttt
     8761 actgccaaat ttacaggggc aattggtttt gtaattccag ttcagccctc tgaagacgtt
     8821 gatcacccct cgctcaaaca cctcatcctt attctggtgc agttgttcaa atgtgctcag
     8881 aatgttcgct tcgctgattt ccggaaaatt atcgtactcc agtgaccggt accatgtatc
     8941 acgggcctgc gtatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat
     9001 ttcgcgatca agattccggg taatggcttt cattgctgtt tcgggtttct ccatcagcca
     9061 gcagccgcag cgaaaatcct gtttcatggc ccagtccggg gcagttttac ctccgatgct
     9121 ttccgtcagc accgagattt cagccagttg tcggataagt gtgtcgattt gcaacagtgt
     9181 actctttcgt acagccagaa tgtggcgaat gctggttgag cagataacgt ccgtatgccc
     9241 gttgaccact tccggacggg agattgtgat atctgacata atcgatattc ctttttgcag
     9301 ataaatgaaa acgccagccc tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga
     9361 gcttcaccgg ttctggtgca gaagctgcag atgacgcagg gtacggacat cctcccgggc
     9421 ctgttcgata cagggcaaca gtgctgctga atccgtttta tcctcatcgt tgtcaaagat
     9481 aattcccgat ccgcagtcga tattgtcctg cagccacgca atcagaatat ccagcgctgt
     9541 ttccgtggtt aatgatttca tgttgttcct ggttaccagt cgaaagtggg taaacccggc
     9601 aggcattcag cgctggcatc cagatgaatg agactgacgc cataacgccg gatgagtgtg
     9661 acgaccagac gacggaacgt ttttgacagc cccaggcgtt taagccgcag aaccgggtat
     9721 gaccacgcat cggtacgtaa cagatatccg ctacctgtaa aatgaatcca ttctgattca
     9781 ccgaagtcac tggtctggtg tgacagcgag tacaaccagg cgttgtccgt ttccgtgata
     9841 tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag
     9901 gtctggtcag attcatcaac aacacgaagg gagtagccgt tatcgcagac catgttaatc
     9961 agttcagcga gctcaatacc atcgacgtca acgacaatgc gccctatgtt cagtgcctgt
    10021 acgttaacgc tgtcggcttc cagtgtcagg gccagtttca tcatttcgcc tccggatact
    10081 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc
    10141 ggcgagcccg gaggatatca atactgttta ttaactgaga gcggggacag gtgctgaatc
    10201 ccggctggtc ggtacgcacc agcgcgtatt tttccacgag aaaattaact gcatcacaca
    10261 gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcgacaaac ggtgtgtcat
    10321 tcagtgtgag gccataatgc tggtccagca gtcggatgag cagtgtctgc cagatggtga
    10381 cgggagacgg gcagcgagat acctcccgta catgtgtgtc ggataatgtt ttcatcttaa
    10441 gttctctgta actggtagtg ctaaaggtga gagcttcggg gttatgaggt ggttgcgggt
    10501 gctggtgtcg gataaacagc cagataaacg taaccacagg agccgagggt gtcggcttcg
    10561 caggtcagcc cttttgcata cagcgtgacg gtatgctgat atcggggatt cagttcaccg
    10621 ctggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc cagatggtat
    10681 gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt cggcaaggta atacaggcgg
    10741 ttaccctctt gcaccagacg agcgccaaaa cagggcctca ctgtgcaggg aagcccccac
    10801 caggggcggt cgtgattgtc atcgggatga gttatcccgg agagtttgtc tgacacgata
    10861 aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg
    10921 accggtttcg gtgaagatgt atgcattgat gagaatggcc tcatccacag cggcgtctga
    10981 ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc agttcagggt gacactcatc
    11041 agccggacat acgggccaaa actgtcctta cggcgttctg caaacacggc aagaacgccg
    11101 ggaatatcct gcacttcacg accagtatac gcttcagcac tgccgtgcca gcgatactta
    11161 ccggtacaga acggaaaaag acgggatgca ggatgctgtt ggtgaatacg catggcttca
    11221 ccacgggtga tgattctcat gatgtgatac ctctgaagac agaagataaa agtgaaaaca
    11281 ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggt
    11341 tgccaccgac tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca
    11401 gacgttcggt gataagccgg tctgccttac tgggtgtgac ttcaccggac gggtgattgt
    11461 gcgccagcac cacggcagcg gcattgtggt acagggcgcg tttaatcact tcccggggat
    11521 gaacttccgt acggttgatg gtgccggtga agagggtttc accggcaatc agctgattct
    11581 ggttgttcag atacagcacc cggaactctt cccgctccag tcccgccatg ttcagaatca
    11641 gccattcccg tgccgcacgg gtggaggtga aggccacgcc gggttcatga agatggcggt
    11701 ccagggtttg cagggcccgc agaatgagac tgcgctcgcc gggcgtcatg tttccgggca
    11761 gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc
    11821 tgcattccgg atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat
    11881 aacactccgt acgacaggca tgatgactgt acgtcatcag acaggcagca atgccggcgg
    11941 cttccgggct catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgt
    12001 catcatcgtc atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat
    12061 tccagatacc accgcagtag tcttcacaca gacggtccat ccagccgaag acacggggtt
    12121 ccagggtgac ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt
    12181 catcagggac gggagtggca accagttgtt gcaggccagt ctccggtgca caggccgaag
    12241 cggtggtgtt ctgagacaat gttttcatgt atgaaatcct tgtaatgtga ataaatgaga
    12301 gggagtaaag gtcgggagct ggcaacatca cgttcatcac tgaccgtgat gacccggtaa
    12361 tatcagggat gaatgatatt cggctgaaat tccggacagc cgtacgcgta accgttccgg
    12421 tcacagacgg atggccagat gccattgcct gtatactcag tctgcacaga ctgatttctc
    12481 cgggacggta aagcagacgg gtacaccagg cgcacaggtc atcatcagaa atggaacgac
    12541 accccggcag cgcctccggg gcgtcgggtg attctggtgc ctgaatcacg ccttcaggga
    12601 catgctgcct cagcgcaggc tctccagcag cgtttctgcc attacccaca atgcgcggtt
    12661 gagcttaata tcggtgtcga tgttgtgaat ggcacgggtg tggatacgtt ttccttttgc
    12721 actgcggccg gaaatcccgc ctttcagcat attctcctgg atggtctgat atgcgctcca
    12781 caggtcttta ccgtaatcct ccctgcgtcg tggtgtcaga atgtcagcgg tggtgactgg
    12841 ctgatgttcg tcaccataac ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg
    12901 tggcggcagg accagcgact gcatggcatc acgcttctcc tctatccggt caaacacgcc
    12961 caccacctcg taagcccctt caataacttt ctccaccaca tttccccggt gtggaacacg
    13021 caattctccc agagactgac cgcagaccag tccattggta caaatggcac gaaaatatcc
    13081 gggtaacatc tggtagctgg atgaaccgtc atgagagttg agcagaataa tttcagggac
    13141 atgctgaccg gttatctgtc cggcccgccg cagacgcagc atgtgttttg tgtattcccg
    13201 gcgacccggg tcacgcacac gtgtctggca ggcgaagaat ggctgaaagc cttcccgctg
    13261 caggctttcc agtacggtga tggtgggagt gtacgcatag cgtttactgc gggaagtatg
    13321 ccggtcttca ccgaaaatac tgggaacata gtgcatcagt tcttcgtgtg tcagcggacg
    13381 gtcacggcgt atctggtttg cagcataacc aaaacgactg gctagtcgca taatttgctc
    13441 cttatcggtg gttaagattt actggtgtaa taaatgaaaa agccacgtct cccggagaag
    13501 acgcggcctg atggacgaaa ttaatgaggt ttattgtctg agaagaccgt cactggcgaa
    13561 ccttccggcg tcagtctctg cgttgcggat aacccgacgg aaatgagttc cctggtcatc
    13621 gtcgataaac acattgcggt aagctgttgt gatgacaaca gcctgctcgc gtgagaaagg
    13681 gacttcgggc aataaacgtg acatacaacc agacctgtat ttggttccct gaaaatataa
    13741 gtccctgtca tcatggcaga gccgtattta cttttgatat caataaatta ataacgcgaa
    13801 gatgaaccgc ataacttacg cctttaattt gttttgttta cgtagtccct gaccaggtac
    13861 gcgaattcgt gaacgccagt ttcaccaaac caaatcaggt aaccagggtc gatgttgtaa
    13921 tagtaaccaa cgactttatg cgtttttggg ttatagagtg ttacgatata gtcccagccg
    13981 caggcgtggg ctttacaacc gccaccttct acccaggtcg ttccgttaat gtttacagga
    14041 tacgcaccac catcaggacc atccatatag ccgtcttccc caaacattcc gtttaaccca
    14101 acagggttta atgctttgtc gatagcagct ctgtaggtag cgtcaccttc gtataactcg
    14161 tacgttgttg gacatgcacc aggctggtca gcccgggctt ttgagtcgca tttaagtgtc
    14221 tgatttacag tggcggcgtt tgt
//
LOCUS       DAJTRM010000097        13859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRM010000097 DAJTRM010000000
VERSION     DAJTRM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.097"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..2604
                     /locus_tag="OO322_003225"
                     /pseudo
     CDS             1..2604
                     /locus_tag="OO322_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2614..6260
                     /locus_tag="OO322_003226"
                     /pseudo
     CDS             2614..6260
                     /locus_tag="OO322_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6321..6728
                     /locus_tag="OO322_003227"
     CDS             6321..6728
                     /locus_tag="OO322_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5907999.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7270..8358
                     /locus_tag="OO322_003228"
     CDS             7270..8358
                     /locus_tag="OO322_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5908000.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8369..10648
                     /locus_tag="OO322_003229"
     CDS             8369..10648
                     /locus_tag="OO322_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908001.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10641..11777
                     /locus_tag="OO322_003230"
     CDS             10641..11777
                     /locus_tag="OO322_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5908002.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11774..13777
                     /locus_tag="OO322_003231"
     CDS             11774..13777
                     /locus_tag="OO322_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908003.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3413 a         3651 c         3561 g         3234 t
ORIGIN      
        1 actgcgccga tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg
       61 tagccgggca tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac
      121 acgcagcact ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca
      181 ttatgctaat gacgatgttt atctcgcaac cagcactggc agaacaggtc attccctggc
      241 tttcgacacc cgcagttgca gtgctgaaat catgccagca ccaactgaca cagccctcaa
      301 accatgccag cgccgatcta ctgccagcca tagtagtctc cccaccatgg atttcgaaaa
      361 agaaaaaatc gccgattccg gtgctggatt tagcgccatt aggcatcgag ccaatctgtt
      421 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
      481 tcacggttag ccatgaagaa agtactacca acctgttggc aaggatgggt tttcaacgac
      541 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
      601 attattcaac cttactaagt gaatttaagg tgtttcattc acctaccggg cattattggc
      661 agttggggat tttgacaaca ttgccgctgg aggctggaga aagcagtaaa agcatggaat
      721 gcccttactc tatctccaca taccgatacc gaatacgcca tgttacattt tggactcaaa
      781 ggtttacctg gtttagttaa ctcaattaca cgctatccac aggaagcctt gcccatcacg
      841 aattacttcg cagcgagtga gctggctcct gccgtcgccc gtgccttcaa caaactcaaa
      901 acgctacgcg aaaacgcccg cacctggctg ttgaaatacc cggaacatgc ccttaccggc
      961 ctgctgcctg cggcgctcgg caaagccggt gaagcacagg ataacgcccg cgctgccttg
     1021 cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag aaatcgcccg gcgttataac
     1081 cagtcggaag taacagacgc ggtgaacgct ctgcttgcgc tcgatccctt agataatcac
     1141 ccgacaaaaa tccccactct tccgaccttt tatcagccat cgatctggac gcgcccggta
     1201 ttaaaagcaa acgcccaatc actgccagat aacgccctcc tccacctcgg cgaaatgctc
     1261 cgcttccctc aggaagaggc tctgtatccg ggattattgc aggtgaaaga cgcctgtacc
     1321 gctgactctc tggctgaatt tgcctgggat ctgtttaccg cctggcagac cgctggcgcg
     1381 ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc
     1441 cgcaaactga cgccattaat acgcgcctgg cctggtgaat cccagcataa acgcgccacc
     1501 gttgggttgg atattctcgc cgctatcggt agtgatatcg ccctgatgca gcttaacggc
     1561 attgctcaga aactgaaatt caaagcatta caggagcggg caaaagaaaa gattgccgac
     1621 attgccgaga gccgcgaact cacggtagcg gagcttgaag atcggttagc accggatctc
     1681 ggtctggatg ataacggttc gttgctgctg gattttggcc cacgccagtt caccgtcagc
     1741 tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg
     1801 ccaaaaccaa acaaaagcga ttatgagtca caggcgaacg atgctgttaa ccgctacaaa
     1861 ttgctgaaaa aagatgcgcg taccgtcgcg gcccagcagg tagcaaggct tgaatccgcc
     1921 atgtgcctgc gtcgccgctg gtcgccggaa aacttccaac tcttcctggt tgtgcatccg
     1981 ctggttcgcc acttaacccg ccgtctgatt tggggcgttt atagcgccga caaccagcta
     2041 ctggcctgtt tccgggtagc ggaagataac agctacagca ccgctgacga tgatcttttc
     2101 accctgccgg aaggcgatat ctctatcggc attcctcacg ttctggaaat atcaccgacg
     2161 gatgctgtcg cctttggtca gctttttgcc gactacgaac tgctaccacc gttccgtcag
     2221 cttgaccgta acagttacgc cctgacagaa gccgagcgca acgccagcga actgacccgc
     2281 tgggcaggca gaaaatgccc gagcggccgg gtcatggggc tggcaaataa aggctggata
     2341 aagggcgaac cacaggatgg aggctggatg atcaaacctc tcggtcgctg gtcgttaatc
     2401 atggaaatcg acgaaggttt tgcggtgggt atatcaccag ccgaacttag cgctgagcag
     2461 ctcttaagca agctgtggct atgggaaggc aaagcggaaa gctatggctg ggggagtaat
     2521 tcaacacagg aagcgcagtt ctccgtactc gatgccatca ccgccagcga gctaattaac
     2581 gatattgaag ccctgtttga ataaggaaag agcatggaca aggaattacc gtggctggcg
     2641 gataacgccc aactggaact gaaatataaa aaaggcaaaa caccactcag tcatcgcaat
     2701 tggccgggcg aaccagtgcc tgttatcact gaaagcatca ttcagacact gggtgatgaa
     2761 ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc gttatgaaaa tttttcaccg
     2821 gagtggcagt ccgccattac gcaggccatc aacttgatcg gcgaacacaa accctcagtc
     2881 ccggcccgca caatggcggc actagccttt atcgcgcaaa atgacaacca acagttgctc
     2941 gacgaaatcg tccaacaaga ggggctggaa tatgcgactg aggtggtgat tgcacgccag
     3001 tttattgcac ggtgttatga gagtgatcct ctggtagtca cattgcagta tcagaacgag
     3061 gactatggct atggttatcg ctcagaaacc tataacgaat tcgatctccg actgcgtaaa
     3121 cacctctctc tggcagagga aagctgctgg cagcgttgcg ccgacaaact cattgccgca
     3181 ctgccaggaa taaccaaagt tcgccgccct tttattgcgc tgatcctccc cgaaaaacca
     3241 gaaatcgcca atgagttggt aagccttgaa tgcccgcgga ctcattttca ttctaaggag
     3301 tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa aactcgaacg ctactggagc
     3361 caggatatat ttagcgatcg agaagccagc tacatgtcgc atgaaaacca cttcggctac
     3421 gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag tcgttccgcg cctcgcgata
     3481 tatgcccata aagaagattg cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc
     3541 cgcaccttgc tgttagttgc tgataaaaac aaacccagcc tgcaacgtgt ggctaaatac
     3601 agtaaaaact tcccccatgc gacactcgcc gcactggcag aactgctggc gttaaaagaa
     3661 ccaccagccc gcccaggtta tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa
     3721 gctcgagatg aatactggcg tacgctgtta caaacgctga tggcatcgca gccacaactg
     3781 gcagcagaag tgatgccgtg gttaagtact caagcccggg cagtgctgaa tagttattta
     3841 tcggcaccgc ccaaaccagt tattgatagc accgataaca gccaaatgcc tgaaatcctc
     3901 gtttcaccac catggcgtgg gagaaagaaa aagacttcca tacgtcactt ttcgctgcct
     3961 gatcttacgc ttgcaccgca aacgtacagg tcgccgaata aacactggac gccggaacag
     4021 caaactgcaa ttgaccattt tagcgccatg tcttttgacg aacgtcttgc tacgagagga
     4081 gcggatatct tcttgcggga attaggtttt gacgttcata gcctggaatt taataagcgt
     4141 cttcttaatg gacagttgga tgcctttgat gctcagcaac tgcaagaata cacatcaata
     4201 ggactattgc gtacttatca cgcgcatcag gccaaatcta aacagtaccg ggaagacgcc
     4261 accaatgcat tactggcaca agatagcatt gcgttgatgg gagggtggcg aacttttcag
     4321 cacggatttt actatggtaa tgaacagcgt tgggggattt ggaatctgta ccttatcgcg
     4381 cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa ttgtcgaggc tgattttcat
     4441 tacaccgggg tggaatattt gttaagtgtg ctgggtaccg atgctttgcc tggattaaac
     4501 accgcctttg cccgacatcc aaaagagata tttccgctgc tgattcattt cggcgcaaca
     4561 gaactggccc tgcccattgc ccgcgtctgg cacctttttg cgggccagcg taatctggct
     4621 cgccagtgga ttttacaatg gccggaacat acggctactg cacttatccc tcttgtcttc
     4681 accaaatcta gcgataaaag cgaagctgca ttacttgccc tgcgtttact ttacgaacat
     4741 gggcatggcg aatggctaca aactgtagcc aatcgttggc agcgtacaga tctatggcct
     4801 gccctggaac aattacttaa acagagtcca atagagattt acccggcacg cattccaaaa
     4861 gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc gccttatcac taataatcaa
     4921 cctgttaccg atgacgctct ggaaattatc ggcgaaatgc tgcgctttac ccagggggga
     4981 cgtttttata gcgggctgga acaactgaaa acgttctgcc agccacaaac gctggcagct
     5041 tttgcctggg atctcttcac tgcgtggcaa caagctggtg cccccgcaaa agacaactgg
     5101 gcatttctgg cgttaagtct ctttggtgac gaaagcacgg cacgggatct aacgacacat
     5161 atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg tcagtggcat gaacatcctt
     5221 accctgatga ataatgatat ggcgctgatg cagctgcatc atatatcgca acgggcgaaa
     5281 tcccgcccct tacgtgataa cgcggcggaa tttcttcagg tagtcgcaga aaatcgcggg
     5341 ctaagccagg aagagttagc ggacagatta gtcccaacac tgggccttaa tgatccgcag
     5401 gcgttgagtt ttgattttgg tccccggcag tttaccgttc gcttcgatga aaacctcaac
     5461 ccggttatct ttgatcagca aaacgttcgc cagaaaagtg ttccccggtt gcgcgccgat
     5521 gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa aagggttaaa aaaagatgct
     5581 actcaggtga gcaaaaacct gctcccgcgt cttgaagctg ccctacgtac cacccgacgc
     5641 tggtcgctgg cagattttca ttctctgttt gttaatcatc cctttacccg tctggttacc
     5701 cagcgattaa tatggggggt tatccggcaa atgaaccgcg tcgtttactc aacgcctttc
     5761 gtgtggccgc agagggggag ttctgcaatg cgcaagatga gccaattgac ctgcctgcgg
     5821 acgctctgat tggcattgcc cacccgttag aaatgacagc agaaatgcgc agtgaatttg
     5881 cacagctttt tgccgattac gaaattatgc cgccttttcg ccagttgtcg cgccgcacgg
     5941 tgctgctcac acctgacgag tcaaccagta acagcctgac tcgctgggaa ggtaaatccg
     6001 ctaccgttgg gcaacttatg ggaatgcgat acaaaggctg ggagtcaggt tatgaggacg
     6061 catttgtcta tgacctgggt gagtaccggc tggtccttaa gttttcaccc ggttttaacc
     6121 actacaatgt tgatagcaaa gcgctaatga gcttccgttc tcttcgagtg taccgtgaca
     6181 ataaatccgt cacttttgcc gaacttgatg tgtttgattt gagtgaggcg ttaagcgcac
     6241 ctgacgtcat tttccattaa aacaaagcgg gcagtgatgc gactgcccgt tatctacacg
     6301 acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt tacgattatg
     6361 aggtccctgt acctgaagat ccgttttcct tcagacttga gatccataaa tgctctgaat
     6421 tatttacagg ttccgtctac cgactggaac ggttccggct acgtccaaca tttcatcaac
     6481 gtgatcgaga ggatgttgac ccgctaataa atgatgcgtt gatttatata agagatgagt
     6541 ttattgatga gcaaaaatta cgaggcgaat cacctgaacc tgtaatagta atttttaatt
     6601 gtaaactaca gaatatattc aaccaacaaa tagaattata tactctagat aattcaagtc
     6661 gcagcacatg caacttgaag tatgacgagt atagctttat taaagggggg aacggtattc
     6721 ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc
     6781 cactaatagc tccagtacag caatgagcac ggttaacttt tgtgtcagcg tgtccatccg
     6841 ctctccttat taaggagcac aacaccagca ccttaccctt acactgcctg tcgatactgg
     6901 tttacgtttg ttgagtcgcc atgttgcact ccggatcaga gcactacctg cggcaccacc
     6961 cgtccggtag ccaggacttg aaataggttc gatccggcgc aacttcccaa cagtcaggat
     7021 tataaatctc attatcttaa ttgcaacact gtccagaggt ggttataatt cgttcgttgg
     7081 aacacgatgt tgcacaggca gtggtgttag gcctgaaaat agtaaataaa agaacctgcc
     7141 tcaccagcag ttttttttat ttactgtgat ctgctttcca gatatttttc gctcaaacaa
     7201 ctaatgcgcc aaacatttat tgcgcgtaaa atatcgttta tttcattaat acatttcagg
     7261 gatgaatata tgtcaccgca gaataatcat ctgcagcgtc cgcctgctgc tgtgttatac
     7321 gccgatgaac tggcaaaatt aaaacaaaat gataacgcac cttgcccgcc cggttggcag
     7381 ttaagtttgc ctgcggcccg tgcttttatc cttggcgata gcgcgcaaaa tattagccgt
     7441 aaagtggtga ttagtccctc cgctgtcgaa cgtatgttag tcactcttgc caccggacgc
     7501 ggtttgatgt tggtggggga acctggcacc gcaaaatctc tcctgtctga attactggca
     7561 acctctatta gcggcgatgc cgggttaacc atccagggcg gcgcatctac taccgaagat
     7621 caaatcaagt acggctggaa ctacgccctg cttatcaatc atggtccatc aacggaagcg
     7681 ctggtccctg ccccgcttta ccagggaatg cgcgacggca aaatcgtccg ttttgaagag
     7741 ataacgcgta caccgctgga agtgcaagac tgtctgttag gtatgctttc tgacagggtg
     7801 atgacggtgc cggaactcac tggtgaagcc agtcagctct atgcgcgaga aggctttaat
     7861 attattgcca ctgccaatac ccgcgatcgc ggcgtcaatg aaatgagtgc tgcgctaaag
     7921 cgtcgcttcg attttgaaac tgtctttccg attatggatt tcgcccagga gctggagctt
     7981 gtcgccagtg cttcggcgcg tttgctggcc catagcggta ttccgcataa agtgcctgac
     8041 gcggtactgg agttactggt ccgcaccttc cgcgatctgc gtgccaacgg cgaaaagaaa
     8101 acgtcaatgg atacactaac agcgattatg tccaccgctg aagccgtgaa cgtggcccac
     8161 gccgtaggcg tccgcgcctg gtttttagcg aatcgcgcag gtgagccagc cgacctggtg
     8221 gactgcattg ccggaaccat cgtcaaagat aacgaagaag atcgcgctcg tctgcgccgt
     8281 tacttcgaac agcgtgtcgc cacacataaa gaagctcact ggcaggctta ttatcaagcc
     8341 cgccaccgcc tgccgtgagg aaagatgcat gagcgagccg ttaattgtcg gcatccggca
     8401 tcatagtccg gcctgcgccc ggctggtgaa atcgttaatc gaaagccagc ggccacgata
     8461 cgtgttgatt gaaggccctg ctgattttaa tgaccgggta gacgaactgt ttttagccca
     8521 ccagcttccg gtagctattt acagttattg ccagtatcag gacggtgcag cccccgggcg
     8581 tggtgcctgg acgccatttg ctgaattttc gccggagtgg caggcgctac aagccgatcg
     8641 tcgtattcag gcacaaactt acttcatcga tttgccttgc tgggcgcaga gtgaagaaga
     8701 ggacgattcg cctgatacgc aagaggaaag ccagaccttg ctgctgcgtg ccacccgcat
     8761 ggataacagc gacaccctgt gggatcactt gttcgaagat gaaagccagc aaactgcatt
     8821 accctctgca ctggcgcact attttgccca actgcggggc gactcccccg gcgatgctct
     8881 aaatcgtcag cgcgaagcct ttatggcccg ctggattgga tgggcgatgc agcaaaataa
     8941 tggcgacgtg ttagttgtct gcggtggctg gcacgctccg gcactggcaa aaatgtggcg
     9001 cgaatgtccg caggtaatta acacgccaga attgccgtcg ctggcagatg ccgttacagg
     9061 ttgttatctc accccctaca gtgaaaagcg ccttgatgtg ctggcaggat acctttcagg
     9121 aatgcctgcc ccggtctggc aaaactggtg ctggcagtgg ggcttacagc aggcgggaga
     9181 acaactgcta aaaacggttc tcacccgttt gcgccagcac aagttgcctg cttcgaccgc
     9241 ggatatggct gccgctcatc tgcatgcgat ggcactggca cagttgcgcg gtcatacact
     9301 accgttacgc actgactggc tggatgccat agcaggatcg ctgattaaag aagccctgaa
     9361 tgcgccgttg ccgtggagct atcgcggcgt tattcatccc gataccgatc cgattctgct
     9421 aacgttgata gacacattag cgggtgacgg attcggtaaa cttgcccctt ctacgccaca
     9481 accgcctctg ccaaaagatg tcacttgcga actggaacgt accgcaatct ctcttccggc
     9541 ggagcttacc ttaaatcgct ttaaccccaa tggactagcg caaagtcagg tgttacatcg
     9601 gctggcaata ctggagatcc cagggattgt acgccagcag ggaagcacac tgacacttgc
     9661 aggcaacggt gaagaacgct ggaaattaac ccgcccgctt agccagcatg cggcattgat
     9721 tgaggccgcc tgctttggtg ccacactcct ggaagccgca cgccataaat tagaagccga
     9781 tatgctggac gcgggtggaa tcggcagcat taccacatgt cttagccaag cggcgtcagc
     9841 gggtctggcg tccttcagtc aacaattact ggagcaactc acattattaa tcgcccagga
     9901 aaatcaattt gccgaaatgg gccaggcgct ggaagtgcta tatgccttat ggcggctgga
     9961 tgaaattagc ggtatgcaag gcgcgcagat attacaaacg acgttatgcg cggctatcga
    10021 tcgcacgctg tggctgtgtg aatccaacgg cagaccggat gaaaaggagt ttcacgctca
    10081 cttgcatagc tggcaagcgt tgtgtcatat cctgcgcgat ctacatagcg gtgtgcaact
    10141 ccccggcgtt tcgctttctg cggcggtagc cttgctggag cgacacagtc aggcgattca
    10201 tgtcctggca ctggatcgcg gcgcgactct tggtgcgctg atgcgtctgg aacatcccaa
    10261 cgctagtgcc gaagcggcgc tgacgatgct ggcgcagtta tccccggcac aatccggcga
    10321 ggcgctgcac ggtttgctgg cattagcccg tcatcaactg gcctgtcagc cggcatttat
    10381 cgccggtttc agcagtcatt taaatcaact aagtgatgcc gattttacca atgccctgcc
    10441 cgatttacgc gcggcaatgg cctggctacc gccacgagaa cgcgggacgc tggcgcatca
    10501 ggtgcttgag cattatcaac tggcgcaact tcccgtttcg gcactgcaaa tgccgttgca
    10561 ttgtccaccg caagccattg cacatcatca acaactcgaa cagcaggcac tggcatcgct
    10621 gcaacactgg ggagttttcc atgtctgaac tgaacgatct tctgaccacc cgtgagctac
    10681 aacgctggcg attaattctt ggcgaagcgg cagaaacgac gctttgtggg ctggatgaca
    10741 acgcccggca gatagatcac gcgctggagt ggctgtatgg gcgcgatcct gaacggctcc
    10801 agcgtggtga acgctctagt ggattaggtg gctcaaatct caccactcct gagtggatca
    10861 acagtattca cacgctgttt ccgcaacagg tgattgagcg gctggaaagc gatgccgtac
    10921 tgcgctacgg cattgaagat gtggtgacaa atctcgacgt gctggaacgt atgcagcctt
    10981 ccgaaagcct gctacgcgcc gttttgcaca ccaaacatct gatgaacccc gaagtactgg
    11041 ctgccgcccg caggattgtg cgccaggttg tggaagaaat tatggcgcga ctggcaaagg
    11101 aagttcgtca ggctttttct ggtgtccgcg atcgtcgtcg ccgctcattt attccactgg
    11161 cgcgaaactt tgatttcaaa agtactctgc gcgccaacct gcaacactgg cacccgcaac
    11221 acggcaagtt gtatatcgaa ttcccccgct ttaacagccg cattaaacgc caaagcgagc
    11281 aatggcaact ggtcttactg gttgatcaaa gcggatcgat ggtcgattcg gtgatccact
    11341 ctgcggtgat ggcggcctgt ttatggcagt tacccggcat tcgtacccat ctggtggcgt
    11401 ttgacacaag cgtcgttgat ctcacggcag acgttgccga tccggtagag ttattaatga
    11461 aagtgcaatt aggcggcgga accaatatcg ccagtgccat ggagtatggt cggcaactta
    11521 ttgaacaacc tgcaaaaagc gtcattatcc tcgtgagcga tttttatgaa gggggttcat
    11581 catcattgct gacgcaccaa gtgaaaaagt gtgtccagag cggcatcaaa gtgctgggac
    11641 tggcagcgct cgatagcaca gcaacacctt gctatgaccg cgatacggcc caggcgctgg
    11701 ttaatgtcgg cgcacaaata gccgccatga caccgggcga gctggcatca tggcttgcgg
    11761 agaatcttca gtcatgaatt cactacgtcc ggaattatta gaactgacac cgcaggcact
    11821 gacggcgtta agcaatgccg gttttgttaa gcgcagcctt aaggaactgg aaaatggcaa
    11881 cgtcccggaa atcagccatg agaacagcgc tttaatcgcc accttcagtg acggtgtccg
    11941 tacccagctg gcgaacggcc aggcactgaa agaggctcag tgcacctatg gggccagcgg
    12001 catgtgccgt catcgcgtga tgctggtgtt aagttatcaa cgactttgtg ctaccgccca
    12061 gcctacggga aaagaagagg aatgggatcc ggcaatctgg ctggaagaac tggctaccct
    12121 tcccgatgcc acccgcaaac gcgcacaggc gctggtcgct aaaagcatca ccattgagtt
    12181 gttctgtgcg ccgggtgaaa ttccctctgc ccgcttaccg atgagcgatg tgcgttttta
    12241 ttcccgcagc agtattcgtt tcgcccgttg tgattgtatt gaaggcacac tttgcgaaca
    12301 tgtcgtactg gcagtacagg cctttgtcca ggcaaaagcg cagcaagcgg aattgactca
    12361 tttaatctgg cagatgcgca gcgaacacgt cacatcatct gacgatccgt ttgccagcga
    12421 agaaggcaaa acgtgtcgtc aatatgttca acaactaagc caggcattat ggctgagcgg
    12481 catcagccag ccgcttattc actacgaggc cgctttcagc cgcgcgcagc aagcagcgga
    12541 acgctgcagc tggcgatggg tgagtgaatc gctacggcaa ctgcgcgcca gcgttgatgc
    12601 cttccacgcc cgcgccagcc actatcatgc cggagaatgc ttacgtcagc ttgcggcatt
    12661 aaacagtcga ttaaattgcg cacaagagat ggcccggagc gacagtgttg gtgaagttcc
    12721 tcctgtgccg tggcgcacgg tcgttggctc tggcattgcc ggagaagcaa agcttgatca
    12781 tctgcggctg gtgtctttag gtatgcgttg ctggcaggat attgagcatt atggtttacg
    12841 catctggttt accgatcccg acaccggcag tattttgcac ctttcgcgca gttggccgcg
    12901 aagtgaacag gaagactcac cggcagctac gcgtcggctg tttagttttc aggctggcgc
    12961 actggcgggt gggcaaattg tttcacaagc agcaaaacgc agtgccgatg gcgagctgct
    13021 gttagctacc cgcaaccgct taagcagcgt tgtaccgctg tcgcctgatg cctggcaaat
    13081 gttgagcgcg ccgttacgcc agccgggcat tgtggctttg cgggaatatt tacgccagcg
    13141 tccccccgcc tgcatacggc ctcttaatca ggtcgataac ttatttattc tgccggtcgc
    13201 tgagtgtatt tcgctcggtt gggatagcag ccgccagacg ctggatgcgc aggtaatcag
    13261 cggcgaaggt gaagataatc tgctgacgtt atcactgcca gcctcagcca gcgcgcctta
    13321 tgccattgaa cgcatggcgg cgcttttgca acaaacagac gaccccgtgt gtctggtttc
    13381 tggctttgtc agttttgttg atgggcaatt gacactggaa ccacaggtaa tgatgacaaa
    13441 gactcgtgcc tgggcgctgg acgcagaaac tgcgcctgtg gtggtatcac taccttctgc
    13501 cagcgttctg cctgtgccgt ctactgctca tcagttgctg atgcgctgcc aggcgttact
    13561 tattcaactg ctccataacg gctggcgcta tcaggaacag agcgctatta atcaggcaga
    13621 gttgctggcg aatgacctca ccgcggtggg tttttatcgg ctggcacatg tgttggcaca
    13681 atttcgtaat acagaaagcg aggcacgggt agaagcaatg aataacggtg ttttgctttg
    13741 cgaacaatta ttccccatgc ttcagcaaca aggatgaaat agtgcttttt actaagagtt
    13801 ctactccagt tccggactgc tcacgccacg gtattaggca tatcctatat agcccctgg
//
LOCUS       DAJTRM010000098        13658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRM010000098 DAJTRM010000000
VERSION     DAJTRM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.098"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            11..118
                     /locus_tag="OO322_003232"
     CDS             11..118
                     /locus_tag="OO322_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5908004.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(99..830)
                     /gene="yehW"
                     /locus_tag="OO322_003233"
     CDS             complement(99..830)
                     /gene="yehW"
                     /locus_tag="OO322_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5908005.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(835..1761)
                     /gene="yehX"
                     /locus_tag="OO322_003234"
     CDS             complement(835..1761)
                     /gene="yehX"
                     /locus_tag="OO322_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5908006.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(1754..2911)
                     /gene="yehY"
                     /locus_tag="OO322_003235"
     CDS             complement(1754..2911)
                     /gene="yehY"
                     /locus_tag="OO322_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5908007.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(2918..3835)
                     /gene="osmF"
                     /locus_tag="OO322_003236"
     CDS             complement(2918..3835)
                     /gene="osmF"
                     /locus_tag="OO322_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5908008.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4087..6384)
                     /gene="bglX"
                     /locus_tag="OO322_003237"
     CDS             complement(4087..6384)
                     /gene="bglX"
                     /locus_tag="OO322_003237"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5908009.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            6580..8295
                     /gene="dld"
                     /locus_tag="OO322_003238"
     CDS             6580..8295
                     /gene="dld"
                     /locus_tag="OO322_003238"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5908010.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8333..9265)
                     /gene="pbpG"
                     /locus_tag="OO322_003239"
     CDS             complement(8333..9265)
                     /gene="pbpG"
                     /locus_tag="OO322_003239"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5908011.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9439..10026)
                     /locus_tag="OO322_003240"
     CDS             complement(9439..10026)
                     /locus_tag="OO322_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5908012.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10196..10774
                     /locus_tag="OO322_003241"
     CDS             10196..10774
                     /locus_tag="OO322_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5908013.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(10891..11652)
                     /locus_tag="OO322_003242"
                     /pseudo
     CDS             complement(10891..11652)
                     /locus_tag="OO322_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(11705..13141)
                     /gene="mdtQ"
                     /locus_tag="OO322_003243"
                     /pseudo
     CDS             complement(11705..13141)
                     /gene="mdtQ"
                     /locus_tag="OO322_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            13365..13448
                     /gene="yohP"
                     /locus_tag="OO322_003244"
     CDS             13365..13448
                     /gene="yohP"
                     /locus_tag="OO322_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5908014.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3237 a         3822 c         3452 g         3147 t
ORIGIN      
        1 ggagtgcatt atgcgcatag ctaaaattgg ggtcatcgcc ctgttcctgt ttatggcgtt
       61 aggcggaatt ggtggcgtca tgctcgcagg ttataccttt attttgcgtg ctggctaagc
      121 gcctgcacca gcctttcaaa caggcggtct gcgatgatcg ccgccagtgc caccagtaac
      181 gccccctgga tcacatacgc ggtattaaat ccgctaagcc cgatgatgat gggcgtaccc
      241 agcgtactgg cccctaccgt tgaggcgatc gtcgccgtac caatgttgat aatcaccgaa
      301 gttcgcacgc ccgccagaat caccggagcc gccagcggta gctcgacctt acgcagtcgc
      361 tgacctcgac tcattcccat acctttcgca acttctgtca cgctgggatc aatcgctccc
      421 agcccggcaa gtgtcacctg caggatgggc agcacaccgt aaaggatcaa ggcgataatc
      481 gctggttgca gaccaaagcc gatcaccgga acggcaatcg ccagcactgc gacaggcgga
      541 aaagtctgtc caacggcggc aatagtttcc accagtgggc gaaattccgc gccccacggg
      601 cgagtaacag caattccggc acctgtgcca atgatcaccg caaacaaact cgaaattccc
      661 accagccaga aatgagccag tgccagagct gcaaaacttt cttgctgata aacgggtcgt
      721 ggcagttgtg ggaacaaggc agcaaacagc ggctggctgt aaggcagcca gaaaatcagc
      781 gccacaaaaa gagcaatgag ccagaacagc ggatcgcgca acatcttcat acgcttacgc
      841 ctccaccagc agatcctgaa aatgcagcgt gccgcaaggc tggccctgcg tgttcaccac
      901 cggcagcacc tcgcatcccc gcgcgacaaa cagggagagc gcatcgcgta gcgtcatctc
      961 ttctgccagt gcctcacctt ctgcccgttc ttcgcgacgc acgtaatccg ccacgctacg
     1021 taacgaaagc aggtgcaccc ccagttcact acgtccaaaa aactggcgga caaaatcatt
     1081 cgccggacga gtcagcatcg tcagcggatt cccctgctgc accacttcac cgtgatccat
     1141 caataccaga tgttctgcca gccgtagcgc ctcatcaata tcatgagtga ccagcacaat
     1201 agtgcgcccc agcaaacggt gaatgcgcgt catctcttgt tgcaacgcgc cgcgcgttac
     1261 cgggtccagt gcgccaaaag gttcatccat cagtaagact tgcggatcgg cagccagtgc
     1321 acgcgccact cccacacgtt gctgctgacc accggaaagc tgatgcggat aacgctcacg
     1381 caaatttgac tccagcccca gtagcgccat taattcgtcg atacgatcgt caatccgcgc
     1441 ccgcgaccat ttttgtaatt gcggcacggt agcgatgttt tgcgccacgc tccagtgggg
     1501 gaacaggcca atagattgaa tggcatagcc catccggcgg cgcaactcca gtactggcag
     1561 cgagcgaatt tcttctccgg caaagcggat cactccgctg tcatgctcca ccaggcggtt
     1621 aatcattttc agggtggtgg atttgccgga gccagatgtg ccaatcagca ccgaaaaact
     1681 cccttcctga aaattgagat tgagatcgtt aacggctttt tgtgcgccga acagtttgct
     1741 gacatggcta aattcaatca ttacgtttca ccttcagcag tgcgataagc aaatcgaaca
     1801 gcgcgtcggt aagcaccgcc agaacaatta ccgggatcac ccccagcaac actaaatcaa
     1861 tggcgctgct tagcagcccc tggaaaacca gcgcaccaaa accgcctgcg ccgattaacg
     1921 ccgcaatcac cgccatacct acagtttgca ccatcaccac ccgcaggctg cgcagaaata
     1981 ccggtaacgc cagcggtaac tgaacatgca ggaaacgctg cgccccgctc atccccatcg
     2041 ctctggcgct ctccagcaca tcgcgcggga tctggttcaa accgactacc acgcctcgca
     2101 ccagcggcag caaggcatag agcaccagcg caatcagtgc gggtgtcatt ccggttcctg
     2161 ctatgccgag cttccccagc cacggaaagg ccgtaaccag cgcggcaagc ggcgcaatca
     2221 acaggccaaa gagcgccacc gaaggcacgg tctgaatgac attgagcaga gaaaaaattg
     2281 ccccctgccg cgcagtggaa aagtagcacc agatgcccaa cggcacacca atcactaacg
     2341 caggcagcac cgcaccaaac agcaacgtca gatgttgtgc cagcgcgtcg tcaaacacat
     2401 cctgacggtt ggcgtattct ttcattagtg agagatcgtt aagcgtgccg gagtacagca
     2461 accacagcgg aataatggca atctgcatat gcaacaacca gcgccacagc ggatgcgtgg
     2521 agattcggcg gatggcatcg ctacaggcca gcaatgccag cgccgcagcc agccagaaac
     2581 ctctgccgag gctggtacgc gccagcgcac tgccattttg cgccagttgg gtcgccgcct
     2641 ttccagctcc ccacaccaac aatacgaaga cgaattgcgc cagaatgagt gcaaaaatgc
     2701 tgcctttttt agcggggaca aaacaggctg tcagccaggc gcaaccaacg cccaccagca
     2761 tccagatcgt ttgcggccac agttgccaga gatgaagccc ttcacccgag actaaacgat
     2821 taggcgcgta actgataaac ggcagcgccg ctgcgattgc cgtcagcaac aacagcagcg
     2881 ccagaacagg attaatacgt agataagtca cgggaaatta cttcgcccac cctttttgtt
     2941 tcaggtagtc ggcagccact tttttggcat ccagtccttc cactgcaatg ctggcattca
     3001 gttgctgcaa tgtttttgca tcgaggctgg cgaagactgg ctgtagccac tgtgccattt
     3061 gcggatactc cttcaacacc gattcacgca ccactggtgc aggcgcgtag ataggttgca
     3121 cgccttgcgg atcgcttaag gtttgcagcc ccagcgccgc gaccggaccg tcagtgccgt
     3181 aagccattgc ggcattaacg ccagaggttt gctgggcagc ggctttaatc gtcaccgccg
     3241 tgtcgccacc agccagtgac agcaactgat cctgaccgag cttaaagcca taggcttttt
     3301 caaacgtggg taacgcatcg gcgcgttcga taaactctgc cgaggctgcc agtttgaagg
     3361 tgccgccctc ttgcagataa cgactcaggt cggcaagcga agtgagtttg tttttctctg
     3421 ccacatcctg acgcacggcg atagtccagg tgttatttgc aggcgcgggc gtcagccaga
     3481 ttaacttgtt gtgctccgca tcgagctttt tgactttctc gtagccctgc ccggcatttt
     3541 tccacgctgc atcgttttca tctttaaaga agaaagcgcc attgccggta tattccggat
     3601 agatatccag ttcatcggaa gtaatcgccc cgcgccccac aggagtcgtt ccgagttgca
     3661 ctttattcac cgtcggtacg ccgtggcttt cgagtacctg caagatgatg ttgccgagca
     3721 gcgcgccttc ggtatcgatt tttgaaccga ctttaacggg ggaagccgcc tgtagcggca
     3781 ggctcacggc tgccaacata accagtgaac ctgcccagag ctttaagagt ggcatgatgc
     3841 tttcctcatt cttttactgt tgttttcagc gaattaagag aaaagcatag ttgataatga
     3901 cagagttagc ctgagcggaa ggattcagtt gcagaatcag ataaatactt aagaggcata
     3961 ttcggtccgg cgtttcctgc cggatgcggc gcgagcgcct tatccggcct acaaagggcg
     4021 aaaattcagc aagttaagat atgaacatca ggcaaagggc gcaaacgtcg cgcccttact
     4081 aaagcattac agcaactcaa actcgccttt cttaacgcgt gcggaatcag tgccgataaa
     4141 tacattgaac ttgccaggct cggcgtcata tttcatctgt tgattccaga acttcagcgc
     4201 ctcaatatcg atcgggaagc tgacagtctg agtttcgccc ggtttcaggg tgattttctc
     4261 aaagcctttc agctgtttca cagggcgact catggaagcc gtcacatcct gcaagtacat
     4321 ctgcactacc gtggccccct cgcgcttacc ggtgttcgtc acctgcacgc tggccgtcac
     4381 tttgccgtca cgcttcatgg tcggcgcaga aagtttcaca tcagagacgg tgaaagtggt
     4441 atagctcaga ccatagccga acggataaag cgctccgtta gcttcatcaa aataacgcga
     4501 agtatatttg ttcggcttgt cggcattata cggacgaccg gtgttcagat ggctgtagta
     4561 caccgggatc tgcccgacag aacgcgggaa ggaaattggc agcttgccgg acgggttgta
     4621 atcgccaaac aatacatcgg caattgcatt accgccttca gtccccgcaa accaggtttc
     4681 cagaatcgca tcagcctgct gatcttcttt caccagcgcc agcggacgcc cgttcatcag
     4741 caccagcacc agcggtttac cggtggcttt cagcgccgca atcaagtcac gttggccttg
     4801 cggaatagtg atatcggtcc ggctggaggc ttcgtgcgcc atcccctgtg cttcaccgac
     4861 tacagccacc accacatcag attgtttcgc cgtctgcacc gcttcatcaa tcatctcttg
     4921 cggcgaacgc ggatcgactt tgaccgcttc ttcatactga ttcaggaaat cgataatgcc
     4981 tttgtcactg gtaacgttcg cccctttggc atacagcact ttaccgtttt caccgacgga
     5041 atttttaatc ccggtcagta cggtcacgga ttgatcggca acaccggctg cggaccagct
     5101 gcccatcacg tcacgtttac tgtccgccag tggcccaacc accgcaatgg tggccgattt
     5161 tttcagcggt aacgtttcga gacggttttt cagcaacacc aggctttcgc gcgccacttc
     5221 acgcgcttct ttacggtgca ggcggctttc ggcattggta tccaccgggt cagactcttt
     5281 cggccccaaa tggctgtatg ggtcgttaaa caaccccata tcatatttaa cgttcagtac
     5341 atggcgggca gcatcgtcca gctctgccat ggtcactttg ccggatttaa tcaacccagg
     5401 cagatacttc gagtagtact cgtcgctcat actcatgttg attccggatt tcagcgccac
     5461 gcgcaccgca tcttccgggt ctgccgccgt gccatgttta atcagctctt tgattgcacc
     5521 gtgatcggaa acggtgatgc ctttaaagcc ccactggtcg cgcagaacat ctttcagcag
     5581 ccaggaatcg gaggtggctg gcgtgccgtt cagcgagttc agcgccacca tcaccgcgcc
     5641 gctgcctgcg tccagccccg ctttgtacgg cggcatataa tcattaaaca ggcgctgcgg
     5701 actcatatcg acggtgttgt actctttacc gccttctacc gcgccgtatg cggcaaagtg
     5761 tttgacgctg gtcatcaccg agtaacgatc tgccgggctt ttaccctgca tcgcttccac
     5821 catggtttta cccattattg aggtgagata cgtatcttcg ccaaaacctt cggaagcacg
     5881 tccccagcgc ggatcgcgcg agacatcgac catcggtgcc caggtcatat tcaggccatc
     5941 atctgccgct tcataagcag agacacgccc gaccgttttc accgcatcga ggttaaaaga
     6001 cgaggccaga ccgaggctaa tcgggaacac cgtgcgctga ccgtgcagca cgtcgtaagc
     6061 aaagaaaaga ggaattttca ggcggcttaa ttccatcacc tgatcctgca tggcgcggat
     6121 atcctgacgg gttacggtgt tgaaaatcgc cccaacctga ccgtctttga tcatctcgcg
     6181 gatcgcctct ttcggattat ccgggcctac gctgattaag cgcagctgac caattttctc
     6241 atcaaccgtc attttcttga gcagatcggt gacgaacgca tcccgcgctt caggcgttaa
     6301 tggatggtta ccgaataaat catccgccag tgctggctgc aggaccagac tcaccgcgat
     6361 tcctactgaa catagccatt tcatatggat ttttcttcct ttgttgcccg acgtggcagc
     6421 gaaaatggtg caaaaaccgt agtttgccat aagcatgatg gagagagaaa aagaatgctc
     6481 agtttattgt ctgaattttc aaaatattca ctcgctgaat tgtcatacaa ggcgctattc
     6541 tagtttgtga tattttttcg ccaccacaag gagtggaaaa tgtcttccat gacaacaact
     6601 gataataaag cctttttgaa tgaacttgcc cgtctggtcg gtcattcaca cctgctcacc
     6661 gatcccgcaa aaacggcccg ctatcgcaag ggcttccgtt ctggtcaggg cgacgcgctg
     6721 gctgtcgttt tccctggctc actactggaa ttgtggcggg tgctgaaagc ctgcgtcacc
     6781 gccgacaaaa ttattctgat gcaggctgcc aatacaggcc tgaccgaagg atcgacgcca
     6841 aacggtaacg actatgatcg cgatatcgtg atcatcagca ccctgcgtct cgacaagctg
     6901 cacgttctcg gcaagggcga acaggtgctg gcctatccgg gcaccacgct ctattcgctg
     6961 gaaaaagccc tcaaaccgct gggacgcgaa ccgcattcgg tgattggatc atcgtgtata
     7021 ggcgcatcgg tcatcggcgg tatttgtaac aactccggcg gctcgctggt gcaacgtggc
     7081 ccggcgtata ccgaaatgtc gttattcgcg cgtatagatg aagatggcaa actgacgctg
     7141 gtgaaccatc tggggattga tctgggcgaa acgccggagc agatccttag caagctggat
     7201 gacgatcgca tcaaagatga cgatgtgcgt cacgatggtc gtcacgccca cgattatgac
     7261 tatgtccacc gcgttcgtga tattgaagcc gacacgcccg cacgttataa cgccgatccg
     7321 gatcggttat ttgaatcttc tggttgcgcc gggaagctgg ccgtctttgc ggtacgtctt
     7381 gataccttcg aagcggaaaa aaatcagcag gtgttttata tcggcaccaa ccagccggaa
     7441 gtgctgaccg aaatccgccg tcatattctg gctaacttcg aaaatctgcc ggttgccggg
     7501 gaatatatgc accgggatat ctacgatatt gcggaaaaat acggcaaaga caccttcctg
     7561 atgattgata agttaggcac cgacaagatg ccgttcttct ttaatctcaa gggacgcacc
     7621 gatgcgatgc tggagaaagt gaaattcttc cgtccgcatt ttactgaccg tgcaatgcaa
     7681 aaattcggtc acctgttccc cagccattta ccgccgcgca tgaaaaactg gcgcgataaa
     7741 tacgagcatc atctgctgtt aaaaatggca ggcgatggcg tcggtgaagc caaatcgtgg
     7801 ctggtggatt atttcaaaca ggccgagggc gatttctttg tctgtacgcc ggaggaaggt
     7861 agcaaagcgt ttttacaccg tttcgccgct gcgggcgcag caattcgtta tcaggcggtg
     7921 cattccgatg aagtcgaaga cattctggca ctggatatcg ctctgcggcg taacgacacc
     7981 gagtggtatg agcatttacc gccggagatc gacagccagc tggtgcacaa gctctattac
     8041 ggccatttta tgtgctatgt cttccatcag gattacatag tgaaaaaagg cgtggatgtg
     8101 catgcgttaa aagaacagat gctggaactg ctacagcagc gcggcgcgca gtaccctgcc
     8161 gagcataacg tcggtcattt gtataaagca ccggagacgt tgcagaagtt ctatcgcgag
     8221 aatgatccga ccaatagcat gaatccgggg atcggtaaaa ccagtaaacg gaaaaactgg
     8281 caggaagtgg agtaaaaatt acggatggca gagtatcgcc atccgaattc acttaatcgt
     8341 tctgtgccgt ctgccccgcc gccgccattt gggcggcttt ttgtttttta tagctcaacg
     8401 ctgctgccgg cacaggcatc actttaccgg tttcaatcca ggtacgcagg cggctggcat
     8461 cggcaaaatg ggtatatttg ccaaacgcgt ccatcactac cagcgccacc ggtttattat
     8521 tgataaccgt acgcatcacc agacaatggc ccgccgcatt ggtaaagccg gttttggtta
     8581 actgaatatt ccagttatcg cgatacacca gatgattagt attgcggaac ggcagcgtat
     8641 acgtcgggtt agagaaggtt gccatatctt cccgggtagt acttaactgc ccgatcaacg
     8701 gatattgttt gctggcaatg agcagtttgg ttaagtcacg ggcagttgaa acgttatgca
     8761 ccgacaatcc ggtaggttca acaaagcgcg tgttgttcat tccgagcgat ttcgctttcg
     8821 cattcattgc cttaataaag gctttgtaac caccgggata atggtgcgca aggcttgccg
     8881 ccgcgcggtt ttctgaagac atcagcgcca gcaacagcat atctttacgg ctgatttcgc
     8941 tattcagtcg tacgcgcgaa tagacccctt tcatctccgg cgtctggctg atatccactt
     9001 ttagtttttc atccagcggc agtcgtgcat ccagcacaac catcgcggtc attaatttgc
     9061 tgatagacgc aatcggacgc accagatccg ggtggttcga atagatcact ttgttggtat
     9121 tcagatcaac aatcatcgcg ctaccggagg caatttccgg ttgtgaagcg gtggtagcgg
     9181 ctgccgtttt cgcaacggcc tgcggtgcaa aaggcacagc cagcatcagg gccaagctaa
     9241 ataaagaaac tcgaaatttc ggcatgatga gcattcagat agtggttcac gcgcacgggt
     9301 tgcgcaccgc cggagtaagg atttactgag gctagcgacg ccatcataac gagcaaaaag
     9361 tgcgatcgtc aaaggagaat cgtgagtaaa tgctgcattg ctgacattta cgccagcaat
     9421 gcaacgtcaa agaactttct agaacaaacg ataaccgtag ccccatagta taacggttag
     9481 ggtgagcagc acttccagta ccagcacgcc aatcgccagc gtcgaactgg agaagctaag
     9541 gccttcctct ttgttgatat tcaggaagct cggaatacca aggtaaagca ggtagccggt
     9601 gtaaaacagc gccaccgtgc cgaccagcgc acacaaccag accagtggat aaagcgccac
     9661 cagaccgctt aaaaacagcg gagttgcaac gtagcccgcg aagaccatgc agtgcgcaag
     9721 agacggacgc tgcgggtaat tgcgcgccat ccaccagatg acccgcccca tcaccgcaac
     9781 cccagccagc ataacgccgt aaaacaagac agccagcgcc agtccggtaa accaggataa
     9841 cttcaggata gtgccatcgc caaaattcca gccaatctgt gtagtgccaa tgaaggcgca
     9901 aatcaccgga atcgccgcca tcagcaaaac gtggtgggtg taatggtgag aaatcgtttc
     9961 gttttcgcga ttaatcacct gcatttcacg atcgggatgg gaaaacagtc cccagacatg
    10021 gctcataccg cccccttgtt gtgagttcat gaacctgaca gttcaagtat aagccagctt
    10081 gtgattattt tttgttcatc catgtaattt gcaccgtctg atgtatgtcc tttttcagcg
    10141 tgaatgattc acagggtgta tgcttacagg caaccaaagg gagacaaact ggcctatgga
    10201 tctcaataca ctcatctcac aatatggtta tgccgcgctg gttatcggta gcctggcgga
    10261 aggtgaaacc gtgactttgc tgggaggcgt tgcggcacat caggggctat taaagttccc
    10321 actggtggta ctttctgtgg cgcttggtgg catgattggc gaccaggtgc tctatctgtg
    10381 cgggcggcgg tttggcggca agctgttacg ccgtttctcg aaacatcagg ataaaattga
    10441 gcgggcacag aaacttatcc aacgccatcc gtatctgttt gtcattggta cgcgctttat
    10501 gtatggcttt cgggtgattg gcccgacgct gattggtgcc agccagctgc cgccgaaaat
    10561 ctttctgccg ctgaatattc tcggcgcatt tgcctgggcg ttgattttta ccactattgg
    10621 ttacgctggt ggtcaggtga ttgcgccgtg gttgcacaat ctcgaccagc atttgaagca
    10681 ctgggtctgg ttgattctgg tcgtggttct ggtggtgggc gtgcgctggt ggctgaaacg
    10741 gcgcgggaag aaaaagccgg ataatcaggc gtaaagcagt tgccacagat aagcattcac
    10801 tccacatccg gcaccgggtg ttccatgcct gatgcgacgc tggtgtctta tctggcctaa
    10861 cccattgaaa tatccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    10921 cataaagccg ccatccacta tcaacgactg ccctgtcgtg taatttgcgc cctccgaaca
    10981 aagccacacc accaggctgg caatctcatg cgttgcgcca aaacgccgta gaggaatcga
    11041 aggctccgcg tcgggcttca cgtcgctatc atccatgctg ttcattggcg tggctatcgc
    11101 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc aactccagcg ccatcgtttt
    11161 ggttaacccg ccgagcgcat gtttagcggc tgtgtaggcg ctggcatccg gcagcggcgt
    11221 atgttcatgt accgacgtaa tgttgatgat gcgaccgccc tgcccttatt tcaccatctg
    11281 acgagccgca atttgcgagc ataaaaatgc accatcaaca tcaacggtaa aaatcttgcg
    11341 ccactcatca aaagccatat caagaaacgg cgctttggtc attgcacccg cattattcac
    11401 cagcacatca atacgcccaa atcgctggat gagtgtctcc agcgcctgcg caccttctgg
    11461 cagattgccg agatccagtt gcacgatctc cgcacgtacg ccgtgggtaa ccacctcacg
    11521 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    11581 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaagcgg taattatcgc
    11641 gacctatgcc atcgagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    11701 atcatcagcg tttgtaaccc ccgcccagcg caccggtgag tcgaatggag gcatccagcc
    11761 actgcccttg caataacagg ccattggccc gctcacgcag cgccgggatt ctggcttcgc
    11821 tgacgcggga accagcaatg atgcccgcgt taaagcgggc ctgcgcaaga cctaccacac
    11881 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgccagt gtctgaacct
    11941 gactggctgc ccgcgccacg tcattcactg cttcaaccac cgctttgttg tagctggcga
    12001 tagacaagtt gctttcggct tttgcgatat cgagattggc gttaagacga ccactatcga
    12061 aaataggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    12121 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    12181 ccgcttttgc cgcatcaata gtgcttaacg atgactcaac gtaccagtgc gccgcctgca
    12241 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    12301 tcggcaacgc gaccggttta agcttcaatg acttagtctg gtgatttgtg agtgcattta
    12361 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    12421 tggcattaat atcggtttcc acaccgtcaa ctgaagaagt aatcccgttc tgatatagct
    12481 ggcgatcggt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    12541 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    12601 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    12661 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gttccgttgg
    12721 tgtaccacgg accggtcgta cctgcggccg gatcgttcag agcaaacggc cccattagcc
    12781 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    12841 gagtggcaac tgccttcgcc tgggcttcag ctagctaaat gcgctgttca gccacctgca
    12901 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    12961 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    13021 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    13081 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    13141 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    13201 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    13261 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    13321 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    13381 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    13441 tattttaaaa ttaattaatg tcatcagatc cgaaaataac gagaatattt cagtctctca
    13501 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    13561 gggggcgacc aatcgccgcc gccccctgcc cccccgggct ctggcgaaca aaatcgccgc
    13621 tgcgcggtgc cctcggctta tcccttacgg ctaccggg
//
LOCUS       DAJTRM010000099        13326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRM010000099 DAJTRM010000000
VERSION     DAJTRM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.099"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OO322_003245"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OO322_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5908015.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OO322_003246"
     CDS             2220..2768
                     /locus_tag="OO322_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5908016.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OO322_003247"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OO322_003247"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5908017.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO322_003248"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO322_003248"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5908018.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO322_003249"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO322_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5908019.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO322_003250"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO322_003250"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5908020.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO322_003251"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO322_003251"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5908021.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12381
                     /gene="pepN"
                     /locus_tag="OO322_003252"
     CDS             9766..12381
                     /gene="pepN"
                     /locus_tag="OO322_003252"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5908022.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12588..>13326)
                     /gene="ssuB"
                     /locus_tag="OO322_003253"
     CDS             complement(12588..>13326)
                     /gene="ssuB"
                     /locus_tag="OO322_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5908023.1"
                     /translation="PLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVGRSGGGKSTLL
                     HLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVGLGLKGQWRDA
                     ARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPLGALDALTRLE
                     MQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVDIPRPRRLGSV
                     RLAELEAEVLQRVMRRGHSEQLIRRHG"
BASE COUNT         3373 a         3374 c         3369 g         3210 t
ORIGIN      
        1 ataacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggtct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag
     1201 atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta
     1441 acaacgtagt ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc
     1501 tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat gggaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg agctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 acgcttacag cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgctgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga
     4381 agtcaagggt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc
     4501 tggtattttt tgccaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca aggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gcataaaaaa aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagttttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc cataggaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagagt acgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgcg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcgttttac gcttaagatc attaatgaaa taagcctggc ggcgaatacg
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaatcaa atatcccttc ctgctttcca atggtaaccg cgttgcgcaa
    10261 ggcgaactcg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgatacctt taccacgcgt
    10381 tctggtcgcg aagtagcact ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt tggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccggttg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggt aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatgg tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catagacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg taatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggtggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct ttacgagaag
    12361 ataactaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ctaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctgccg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actgaccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtatcatcct gaatttcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac cggccagcag gtgcagcagg
    13201 gtacttttac caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggt
//
LOCUS       DAJTRM010000100        13251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRM010000100 DAJTRM010000000
VERSION     DAJTRM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.100"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(17..999)
                     /gene="kdgT"
                     /locus_tag="OO322_003254"
                     /pseudo
     CDS             complement(17..999)
                     /gene="kdgT"
                     /locus_tag="OO322_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO322_003255"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO322_003255"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5908024.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OO322_003256"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OO322_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO322_003257"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO322_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5908025.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO322_003258"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO322_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5908026.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OO322_003259"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OO322_003259"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5908027.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OO322_003260"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OO322_003260"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5908028.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OO322_003261"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OO322_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OO322_003262"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OO322_003262"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5908029.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="OO322_003263"
     CDS             9657..10103
                     /locus_tag="OO322_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5908030.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="OO322_003264"
     CDS             10114..11565
                     /locus_tag="OO322_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5908031.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="OO322_003265"
     CDS             11555..12625
                     /locus_tag="OO322_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5908032.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13251
                     /locus_tag="OO322_003266"
     CDS             12625..>13251
                     /locus_tag="OO322_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5908033.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQY"
BASE COUNT         3160 a         3458 c         3491 g         3142 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggacca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaataatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaata t
//
LOCUS       DAJTRM010000101        13097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRM010000101 DAJTRM010000000
VERSION     DAJTRM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.101"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..890)
                     /locus_tag="OO322_003267"
     CDS             complement(<1..890)
                     /locus_tag="OO322_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5908034.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO322_003268"
     CDS             1131..1931
                     /locus_tag="OO322_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5908035.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO322_003269"
     CDS             2009..2779
                     /locus_tag="OO322_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5908036.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO322_003270"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO322_003270"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5908037.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO322_003271"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO322_003271"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5908038.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OO322_003272"
     CDS             5034..5756
                     /locus_tag="OO322_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5908039.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO322_003273"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO322_003273"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5908040.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO322_003274"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO322_003274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5908041.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OO322_003275"
     tRNA            7149..7225
                     /locus_tag="OO322_003275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OO322_003276"
     CDS             complement(7356..8402)
                     /locus_tag="OO322_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5908042.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO322_003277"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO322_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO322_003278"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO322_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5908043.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO322_003279"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO322_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5908044.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OO322_003280"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OO322_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO322_003281"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO322_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3213 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtg
//
LOCUS       DAJTRM010000102        12685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRM010000102 DAJTRM010000000
VERSION     DAJTRM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.102"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO322_003282"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO322_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5908045.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO322_003283"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO322_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5908046.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMEFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO322_003284"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO322_003284"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5908047.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO322_003285"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO322_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5908048.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO322_003286"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO322_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5908049.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO322_003287"
     CDS             4025..4615
                     /locus_tag="OO322_003287"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5908050.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO322_003288"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO322_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5908051.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO322_003289"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO322_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5908052.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO322_003290"
     CDS             complement(6551..6658)
                     /locus_tag="OO322_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908053.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO322_003291"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO322_003291"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5908054.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO322_003292"
     CDS             7648..7875
                     /locus_tag="OO322_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5908055.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO322_003293"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO322_003293"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5908056.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO322_003294"
     CDS             complement(9447..10703)
                     /locus_tag="OO322_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5908057.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     EPFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO322_003295"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO322_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5908058.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO322_003296"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO322_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5908059.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12685)
                     /gene="torD"
                     /locus_tag="OO322_003297"
     CDS             complement(12282..>12685)
                     /gene="torD"
                     /locus_tag="OO322_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5908060.1"
                     /translation="RDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVE
                     AGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEF
                     AARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3030 a         3236 c         3197 g         3222 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggag ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cctgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaggttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgta
//
LOCUS       DAJTRM010000103        12662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRM010000103 DAJTRM010000000
VERSION     DAJTRM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.103"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO322_003298"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO322_003298"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5908061.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO322_003299"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO322_003299"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5908062.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO322_003300"
     CDS             3115..3399
                     /locus_tag="OO322_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5908063.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO322_003301"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO322_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5908064.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO322_003302"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO322_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5908065.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO322_003303"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO322_003303"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5908066.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO322_003304"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO322_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5908067.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="OO322_003305"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="OO322_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5908068.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="OO322_003306"
     CDS             10849..11334
                     /locus_tag="OO322_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5908069.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="OO322_003307"
     CDS             11444..12433
                     /locus_tag="OO322_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5908070.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3340 a         3128 c         2942 g         3252 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcga ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DAJTRM010000104        12662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRM010000104 DAJTRM010000000
VERSION     DAJTRM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.104"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(270..989)
                     /locus_tag="OO322_003308"
                     /pseudo
     CDS             complement(270..989)
                     /locus_tag="OO322_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1005..1337)
                     /gene="cusF"
                     /locus_tag="OO322_003309"
     CDS             complement(1005..1337)
                     /gene="cusF"
                     /locus_tag="OO322_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5908071.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1361..2743)
                     /gene="cusC"
                     /locus_tag="OO322_003310"
     CDS             complement(1361..2743)
                     /gene="cusC"
                     /locus_tag="OO322_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5908072.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2900..3583
                     /gene="cusR"
                     /locus_tag="OO322_003311"
     CDS             2900..3583
                     /gene="cusR"
                     /locus_tag="OO322_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5908073.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3573..5015
                     /gene="cusS"
                     /locus_tag="OO322_003312"
     CDS             3573..5015
                     /gene="cusS"
                     /locus_tag="OO322_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5908074.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5165..7402
                     /gene="nrfB"
                     /locus_tag="OO322_003313"
     CDS             5165..7402
                     /gene="nrfB"
                     /locus_tag="OO322_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5908075.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7389..10357
                     /gene="nfrA"
                     /locus_tag="OO322_003314"
                     /pseudo
     CDS             7389..10357
                     /gene="nfrA"
                     /locus_tag="OO322_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10358..11248
                     /locus_tag="OO322_003315"
     CDS             10358..11248
                     /locus_tag="OO322_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5908076.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11431..12192
                     /gene="envY"
                     /locus_tag="OO322_003316"
     CDS             11431..12192
                     /gene="envY"
                     /locus_tag="OO322_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5908077.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12238..12314)
                     /locus_tag="OO322_003317"
     tRNA            complement(12238..12314)
                     /locus_tag="OO322_003317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12278..12280),aa:Arg,seq:tct)
     gene            12557..>12662
                     /locus_tag="OO322_003318"
     CDS             12557..>12662
                     /locus_tag="OO322_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR5908078.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKT"
BASE COUNT         3099 a         3284 c         3220 g         3059 t
ORIGIN      
        1 attcttgtgg gccagaatat ggtttaccat aaatagtacg cagttttgtg tcgcttatag
       61 ccataaataa tatttggtac gctcagaaat aatggtttgg tacacatctg gtacacaata
      121 tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac atactcaggt
      181 gaaaaaaagg ttgtttttcg agttataaca gacagttaat taactacagt ggacaatgct
      241 agacaatgac agacacagat aagcaaccta ccttcctctt tcgcgaccac gttatctttg
      301 gcgatattca ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat gggcgctttg
      361 agcgtaaagc gagtctggat tttttgcgtg gcgatcaggt ggcgaatatc cgcctccggc
      421 attcccgcca gtcgcagccg ctcaagaatg ccttcagtct gggtcgccgt accgccggtt
      481 tcgcgcaaca gtaaatactc actctgcgct tccacccagt cgggaatggt caggtcgaga
      541 agcggtgtgc ccttttgtac tttatcgccc acggtaagcg gatacacctt gacgataaac
      601 ccggcagcgc gagcctgcac aatggcatac tgatactcgt tgtaactgac attcgtcggg
      661 aaactctggg caaaagtcag cggcccgcgc gtgacggtag ccgttttcac ccccagattc
      721 tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac tctcttcatc ggcatatttc
      781 ggcaccagat ccatatccat aaacggcgat ttacctggtt tatcgaaccg cgtattggga
      841 tacatcgggt cgtaccagaa taagatttta cgttctgcgg tcggcatttt ttctgcgtgc
      901 ggttccgcct ggtcaaacca ggtaaaacct gccgctgaaa taataccgcc cgcgatcatg
      961 ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg gctgacttta
     1021 atatcctgta ataaagaaag gttgccctgc tggacaaaat taaacgccac tttgtcgccg
     1081 gttttaattt cactcatttt cgtctgcggg gtgatggtaa agcgcatggt catctccggc
     1141 cagttgacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc cagatcaaca
     1201 ccttttacca caccggtggc actaatgacc tgtggttgtg cttcgctcat ggtttcatga
     1261 tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactgaa cattgcgact
     1321 tgcagtgctt ttttcattta ttctctcctg gagttaaaaa ttattgctgc caaccgcccc
     1381 ctaaggcggt atacaaataa atttcgttaa cctgacgggc ataattcagg tcaagtaaag
     1441 tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa cttaccgcgc
     1501 cgtgctgata taatgcccgc gcccgttgca gggtaatttg cagcgacgcc agataacgct
     1561 gctgggcgct gatttgatcg tccaggcttt gacgtaatgc cagcgcatct gccacctctt
     1621 taaaggcgtt ctggattttc tgttcataat tcaccaccga ctgctgctgg cgaatttcgg
     1681 cgatatccag attggcctgg ttgcgtccgg cattaaaaat ggggatctca attttaggaa
     1741 taaaattcca catcccgctg ctagcgttaa ataatgacga cagatcgctg ctggcggttg
     1801 atattccgct ggtcaggctg atagacggga aaaatgccgc acgcgcagca ccaatattag
     1861 cattagccgc cattaacgcg tgttcagctt ccataatatc cgggcgctgc aataagattt
     1921 gcgacgacaa gcccgccggt aatttaacgc tttgcaggct gccgctgttt actgtctgcg
     1981 cttgcggtag cttgccgtag cttcccaata acagttgcaa tgcattattc gcctgcgcca
     2041 gttccccctg acgtttagcg atgtcactgc gggtactttc tatcaccccg cgagcctgtt
     2101 ccagcgccag aacattgctg ctaccggtca acagttgttt ttcgacaaac gcatatgact
     2161 gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc agctgctgat
     2221 tgaaatagct ttgcgcgaca ttagaaacca gcaaaatatg caccgcgcgc tgggcttcct
     2281 cagtggctaa ataattttgg cgctcggctt cgctcatgtt ctttaagcga ccgaaaaaat
     2341 cgagatcaaa gctggcgtta aggccagtcg agaactcccg cgtcgtggct gaatcgcctt
     2401 taagattgcc gctccagctg ccgctgccct cgccattgag ctgtgggtag cggtcggcat
     2461 cggtcagacg atattgcgcc cgtgcctcct gcactttcag cgtcgccatg cgcaaatccc
     2521 ggttattcac cagcgcctcg ctaatcagcg tcttcacctg attatcaaca aaaaaggtgc
     2581 gccagcccac gttctggtag ttatccgctg cgttaaccag gccgttctgg ctgagtgaga
     2641 actgctgcgg tacgggcatt accggacgct gataatccgg tgccagtgaa caaccagtta
     2701 gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca taatttctgg
     2761 tgattttatg tcgccaactt tactcgccag gctctgattt tccggtgaca ggaaaatgac
     2821 aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta gaattgcccg
     2881 ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga aaaccggaga
     2941 atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg acaacgggct
     3001 gaatggctac catctggcga tgaccggtga ttatgacctg ataatcctcg atattatgct
     3061 gccagacgtg aacggctggg atatcgtgcg catgttgcgc tccgccaata aagggatgcc
     3121 gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc tggagttggg
     3181 ggcagatgac tacctggtga aaccgttcgc ttttgctgaa ctgctggcgc gggtgcgcac
     3241 tctcctgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg ccgatctgat
     3301 ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactc tgaccagtaa
     3361 agaatttact ctgctggagt tttttctccg ccatcagggc gaagtgctgc cccgctcgct
     3421 tatcgcctcg caggtatggg acatgaattt cgacagcgac actaacgcca ttgatgtggc
     3481 ggtgaagctg ctacgcggca aaatcgacaa cgactttgag ccgaagctga ttcaaaccgt
     3541 gcgcggcgtg ggttacatgc ttgaggtgcc ggatggtcag taagccattt cagcgcccgt
     3601 tttcgctggc aacccgcctg acctttttta tcagccaggc caccatcgcg gcgtttttcg
     3661 cctttgcatg gatcataatc cactcagtaa aagtgcattt tgccgagcag gacattaatg
     3721 atttaaaaga gattagcgcc acccttgaac gggtactaaa tcaccctgac gaaacgcaag
     3781 cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaacgtg ttgatttccc
     3841 tggcagatag ccacggcaaa acggtgtatc actcccccgg tgcgcctgat atccgcgagt
     3901 ttgcacgtga tgccataccc gataaagacg cgcggggtgg cgaggtgtat ctcctttccg
     3961 gcccgacgat tatgatgcca ggccacggtc acgggcatat ggaacacagc aactggcgga
     4021 tgattaactt gccggttggc ccgttggtgg acggcaaacc gatttatacg ctctacatcg
     4081 cgctgtcgat cgattttcat cttcattaca taaatgattt gatgaataaa cttattatga
     4141 ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct gttggcggta cataaaggtc
     4201 acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa gatctcgacg
     4261 ttcgccttga cccgcagacc gtgccgattg agctggaaca gctggtactg tcgttcaacc
     4321 atatgatcga gcgtattgag gatgtcttta ctcgccagtc caatttctca gcggatatcg
     4381 cccacgaaat tcgcacacca attactaatc tcattacgca aacggaaatc gccctcagcc
     4441 agtcgcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa gagctgacgc
     4501 gaatggcgaa aatggtcagc gatatgctgt ttctcgctca ggccgataac aaccagctaa
     4561 tccccgaaaa gaaaatgctc aacctggcgg atgaagtcgg caaagtgttc gattttttcg
     4621 aggcgctagc ggaagatcgc ggcgtggagt tgcggtttgt tggcgacaaa cgtcaggttg
     4681 cgggcgatcc gttgatgctg cgtcgggcgc taagcaacct gctttctaat gcccttcgtt
     4741 atacgccaac cagagagaca attgtagtac gctgtcagac ggtcgatcac caggtgcaag
     4801 tttccgtcga aaaccccggt acgcccattg cgcccgagca cttaccgcga ttgtttgacc
     4861 gtttctatcg cgttgacccc tcccgccagc gaaaaggtga aggtagcggt attgggctgg
     4921 cgatagtgaa atcgattgtt gtcgcgcata aaggcacggt tgcggtaacg tcagatgcgc
     4981 gggggacaag gtttgttatc acattacccg cttaattatt cagcaaacgg caacttttat
     5041 aaccagtgta aaaataacgt gccgcaatag tcatacatat taatggtaaa aagctgtcac
     5101 aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa tgtaaggttt
     5161 ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa aagtaatcgc
     5221 gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta ttgatgtcgt
     5281 ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc cgcgaatgag
     5341 ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc cggcgtggaa
     5401 tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg actacgaaaa
     5461 ctatcatatc tttgttggca cctaccccaa cgaccccgat actcaacgtg atgttgacga
     5521 agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg gtcccaccag
     5581 caaagccgac tgcctgaaca acgtgctgga cgccatcacc cagtttgaac gtagcgccaa
     5641 tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac cgatggaatt
     5701 gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccag tgtatccgtt
     5761 tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt cagaactgca
     5821 tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg caggcgtcgg
     5881 cacctgtttc agccgccgcg ccgtgaccgc actgttagct gacggtaacg gtattgcttt
     5941 cgacgtgcag agtcttactg aagattacga cattggcttc cgcctgaaag aaaaaggtat
     6001 gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg agcagcgtaa
     6061 atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt tccccgatac
     6121 cttttcgact gcggttcgac aaaaatctcg ctggatcatc ggcattgttt tccagggctt
     6181 taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc gcgaccgcaa
     6241 aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc agcttttgct
     6301 gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta tttttagcgg
     6361 cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta accgcatcgt
     6421 gcagcgagtg attttcgtca ctggctacta cggcctcacg cagggcctgc tttccgtcct
     6481 gcgtcttttc tggggcaacc tgattaactt tatggctaac tggcgcgtgc tgaaacaggt
     6541 tcttcagcac ggcgatccgc gtcgcgtcgc gtgggataaa acaacgcatg acttccccag
     6601 cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg aaaatcaggt
     6661 catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc tacgcctggg
     6721 cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg cgctggcaga
     6781 gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct cgctgattgc
     6841 cgaaatgcca gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc tggaaaatga
     6901 tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg ccctgacgcg
     6961 taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg tcacagggtt
     7021 acgccactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt acaatgcggt
     7081 tcagcatcag tggctcacgg aacagcagac cggtgaaatc tggcggcaat atgtgccgca
     7141 tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt cagcaattaa
     7201 cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt tggtcaccga
     7261 aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg aattacaagt
     7321 ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg cgcaactgga
     7381 gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt ttactgctga
     7441 cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca gaagagctgg
     7501 ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg ctgaaggcac
     7561 agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac caacaggtgc
     7621 cggataatat tccgctgact ttataccttg cggaagccta tcgtcatttt ggtcatgatg
     7681 accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat gcccgacttg
     7741 agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgactgtt gaagaactgc
     7801 ttgcccagca aaaagcgggc gacgctgcgc cgaccctgcg ttgtcgcagt gaagtcgggc
     7861 agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac gatgcgacgt
     7921 ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg gcaatctacc
     7981 tgaaacaatg atcccaggca gatacgctat acaatgaagc acgccagcag aacacattaa
     8041 gcgcggcaga acaccgtcag tggtttgacg tgcttcttgc cgggcagctg gacgatcgga
     8101 tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt acttacgcga
     8161 ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt gaaaataagc
     8221 cgctgtttac cacgggcgca caagagaaaa gttggctcta tctgttatct aaatacagcg
     8281 ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac cgccagtatg
     8341 ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca gcgcaaaaac
     8401 tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc agcgtggcga
     8461 cccgtaacaa ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa gaaccgacaa
     8521 atcttccccg cctggatcaa ctgacctggc aactgatgca gaacgagcag tcacgcgaag
     8581 ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc agccagactt
     8641 taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctagcaacg ccggcgaagg
     8701 tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa agtcagttgc
     8761 cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg tcgccttcct
     8821 acgatgctgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta cccggtgtgg
     8881 cgttgtatgc atggcttcag gccgaacaac gacaaccgag cgcctggcaa catcgtgcgg
     8941 tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc tggcagaaaa
     9001 tcagtcttca cgacatgagc aatgatgatc tgctcgccgc cgccaacacc gctcaggcag
     9061 cgggaaatgg cgcagctcgc gatcgctggc ttcaacaggc agaacaacgt gagctgggaa
     9121 acaatgctca atactggtgg ctgcatgcgc gacgttatat cccagctcag ccagaactcg
     9181 cactgaacga tctcacgcgc tcaatcaata ttgccccttc tgccaactct tacgttgcgc
     9241 gggcgacaat ttatcgccaa cgtcataatg ttccggccgc ggtgagtgat ttgcgcgccg
     9301 cgctggaact ggaaccaaat aatagcaaca cccaggcagc gcttggttac gccttgtggg
     9361 atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa gggcttccgg
     9421 acgatccggc actgatccga caactggcct acgtgaacca gcgtctggat gacatgcctg
     9481 cgacgcagca ctacgcccgg ctcgtgattg atgacattga taatcaggca taaccccact
     9541 gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg
     9601 ccgctggacg ttcagtttcg attcttccat cggcttgcgt tccggcgcaa tgagtaccgc
     9661 taacaataat gtcggcggtg cagcgcctgg gaaaagctat cgtagctacg gacaactgga
     9721 agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct cagtttatag
     9781 ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc
     9841 cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg ccgtcgaaca
     9901 gcagttgccg ctgaacgggc aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc
     9961 attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc
    10021 ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc
    10081 agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg agccttacgc
    10141 tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggtgt
    10201 cggggtccgc tggaatattt ggaccggcga gacgcactac gacgcctggc cgcacaaagt
    10261 cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta acggagagcg
    10321 caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt
    10381 gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc
    10441 acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg
    10501 ttttcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg catttaccca
    10561 gccagaacag cgcacgttat tgtttaagcg tgccgcagct gcgcaacaga ctggcctgaa
    10621 gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtctgc
    10681 agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg
    10741 gagcgccgcg cctggcataa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct
    10801 gaactggcgc agcgaagccg cccgtcaacc tttgctaaca tggttaaaca acgcgcagcg
    10861 gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg ccggaaacat
    10921 gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggag ttaatgtctg
    10981 ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac gttatctaca
    11041 ggccagcgcc gattgccaaa gctccgcccc agcaagcggc attgtttatg aactgtttgt
    11101 cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gacgcagaaa ttgcctcgct
    11161 gttagcgaaa cgttcctctt gcggtaaaga catgctctat ttctctctgc gctatttgcc
    11221 cgtcgcgcac ggcattctcg agtattaaat cccctccagg taagtcgggt acgacctggc
    11281 ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag catgctaaag
    11341 aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata gatcctttga
    11401 gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga accttgcgtg
    11461 gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac gtttaccctt
    11521 ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact ctcaacgtta
    11581 aatcacgtat taatcaccca catcaaccgt aacatcatca acgattatct gttgttttta
    11641 aataagaaat taacctgtgt aaagccctgg tcgcggctag caaccccggt tatcgcttgt
    11701 catagccgta caccggaagt gttccggcta gccaccaacc acagcaagca gcaatccagc
    11761 aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc taactttctt
    11821 gagcaatcgc ggtttattgc cctactgatg tatatcttac gcagcagcgt ccgcgacagc
    11881 gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat tgtcgccagt
    11941 tcgctatgtt taagccccag cctgctcaaa aagaaattaa aaaacgaaaa taccagctat
    12001 agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt gatggataac
    12061 aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta ctttatctct
    12121 gtttttaagg cgttttacag cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa
    12181 gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa gaagaaatgg
    12241 cgcgccctgc aggattcgaa cctgcggccc acgacttaga aggtcgttgc tctatccaac
    12301 tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc
    12361 cttgctgagt caatggcttt tgatctggtt gctgaacaag tgaacgaccg cgtctgattt
    12421 tctgatttat ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct gaatcaagaa
    12481 aacccgtatt ttcatgtatc aaagtacaat ttaccgccct aacggaaaat tgtccgctcc
    12541 tatgagactg gtaactatga aaccaacgtc ggtgatcatt atggatactc atcctatcat
    12601 cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg tcctgaaaac
    12661 gg
//
LOCUS       DAJTRM010000105        12487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRM010000105 DAJTRM010000000
VERSION     DAJTRM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.105"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO322_003319"
     CDS             complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO322_003319"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5908079.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1796..3214)
                     /locus_tag="OO322_003320"
     CDS             complement(1796..3214)
                     /locus_tag="OO322_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5908080.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3368..3712)
                     /locus_tag="OO322_003321"
     CDS             complement(3368..3712)
                     /locus_tag="OO322_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5908081.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO322_003322"
     CDS             complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO322_003322"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5908082.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4702..5637)
                     /locus_tag="OO322_003323"
     CDS             complement(4702..5637)
                     /locus_tag="OO322_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5908083.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5717..6832
                     /gene="yneK"
                     /locus_tag="OO322_003324"
     CDS             5717..6832
                     /gene="yneK"
                     /locus_tag="OO322_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5908084.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6982..8172
                     /gene="ydeA"
                     /locus_tag="OO322_003325"
     CDS             6982..8172
                     /gene="ydeA"
                     /locus_tag="OO322_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5908085.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO322_003326"
     CDS             complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO322_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5908086.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9074..9508
                     /gene="marR"
                     /locus_tag="OO322_003327"
     CDS             9074..9508
                     /gene="marR"
                     /locus_tag="OO322_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5908087.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9528..9911
                     /gene="marA"
                     /locus_tag="OO322_003328"
     CDS             9528..9911
                     /gene="marA"
                     /locus_tag="OO322_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5908088.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9943..10161
                     /gene="marB"
                     /locus_tag="OO322_003329"
     CDS             9943..10161
                     /gene="marB"
                     /locus_tag="OO322_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5908089.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO322_003330"
     CDS             complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO322_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5908090.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11355..12473
                     /gene="ydeE"
                     /locus_tag="OO322_003331"
     CDS             11355..12473
                     /gene="ydeE"
                     /locus_tag="OO322_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5908091.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
BASE COUNT         3279 a         3218 c         2860 g         3130 t
ORIGIN      
        1 caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata ggatgttgta
       61 actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta
      121 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
      181 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
      241 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
      301 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
      361 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
      421 gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
      481 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
      541 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
      601 ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
      661 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
      721 tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata
      781 tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa
      841 gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac
      901 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
      961 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
     1021 cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc
     1081 tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc
     1141 cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
     1201 ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc
     1261 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
     1321 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt
     1381 gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag
     1441 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
     1501 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
     1561 tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt ggtgcgacag
     1621 gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca
     1681 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
     1741 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
     1801 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
     1861 acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg
     1921 atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg
     1981 tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag
     2041 gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct
     2101 tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt
     2161 catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca
     2221 gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt
     2281 aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcaaattga
     2341 tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac
     2401 gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat
     2461 ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg
     2521 gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc
     2581 cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc atcatctgtt
     2641 ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca
     2701 acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac
     2761 tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac
     2821 agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc agacgcttat
     2881 cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca
     2941 gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga
     3001 tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc
     3061 cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat
     3121 agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga
     3181 acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg
     3241 tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt cgctaacagc
     3301 ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct
     3361 gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt
     3421 cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg
     3481 tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt
     3541 cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga
     3601 tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag
     3661 agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac
     3721 cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc
     3781 tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac
     3841 aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc
     3901 gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt
     3961 caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg
     4021 ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt
     4081 tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg
     4141 cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc
     4201 gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa
     4261 catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa taccctgttc
     4321 catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg
     4381 ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat
     4441 agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt
     4501 acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc
     4561 gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt
     4621 atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat
     4681 atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga tattacagaa
     4741 ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa
     4801 ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg
     4861 tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa
     4921 ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgcagc
     4981 aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac
     5041 cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta acaaacgcgc
     5101 accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc
     5161 cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc
     5221 tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc
     5281 cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag
     5341 ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca
     5401 ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag caatgcgcga
     5461 gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg
     5521 cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca tcacattcgg
     5581 cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac
     5641 ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg atgacttatg
     5701 actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat ctcttcctga
     5761 tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg attttattga
     5821 tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca ccaactcagc
     5881 cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga cttcttttcg
     5941 gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc ctgtcaagaa
     6001 tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc ctatttattt
     6061 agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc caaagcctgt
     6121 cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt ttatgaatga
     6181 ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag cgcatacacc
     6241 acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac gccagtttga
     6301 tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc gattatggaa
     6361 taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca ccgccattct
     6421 cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg attcattagg
     6481 ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt tagcagaaca
     6541 agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat tgcactatca
     6601 ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag catcattaag
     6661 tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg taaaaaatga
     6721 aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct cccctgtcag
     6781 accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt aaaccgtgat
     6841 gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa cgcgatccag
     6901 atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat cactttttcg
     6961 caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt ggcgtggcta
     7021 cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt
     7081 ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt cggcatcatg
     7141 ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt aatgaccagt
     7201 caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc cagccacgta
     7261 ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt
     7321 gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc tccggccggg
     7381 aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat ggtcttaggt
     7441 ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt cttcgcgatt
     7501 ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt actgcctagt
     7561 gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc attgatgagc
     7621 atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag ctatatcgag
     7681 ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt actgttatta
     7741 ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa tcaatatgcg
     7801 tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact gctgctacct
     7861 gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat cgcgatgatg
     7921 atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc taccgacgtc
     7981 gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc gttggtaggt
     8041 aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct
     8101 tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac actcgaagaa
     8161 cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt taatgatttc
     8221 caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc ccatcaggcg
     8281 ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg agctgcgtag
     8341 gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac
     8401 ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa tcatcgctat
     8461 ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat tggcactggg
     8521 ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg ctttctgttg
     8581 cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc
     8641 gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg ccaccatcat
     8701 gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg cactgttcat
     8761 gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta acggcagcaa
     8821 caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt gttatcctgt
     8881 gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt cactctttag
     8941 ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg gcatcggtca
     9001 attcattcat ttgacttata cttgcctggg caatattacc ccctacaact aattacttgc
     9061 caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc cattgggtcg
     9121 cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc tgtctccgct
     9181 ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac
     9241 tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc gtatgctgga
     9301 tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata agcgcggcgt
     9361 actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc aattagttgg
     9421 ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg caacacttga
     9481 gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg tccagacgca
     9541 atactgacgc tattaccatt catagcattt tggactggat cgaggacaac ctggaatcgc
     9601 cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac ctgcaacgga
     9661 tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt aagatgacgg
     9721 aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca gaacgatatg
     9781 gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat gttccgccgc
     9841 ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca ttaaatcatt
     9901 acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc actttcatcc
     9961 gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca aaccacgcag
    10021 ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga acaaccaccg
    10081 tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg cgtgccctat
    10141 tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt attaacttcc
    10201 cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    10261 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    10321 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    10381 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    10441 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    10501 atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc tccagattac
    10561 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    10621 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    10681 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    10741 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    10801 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    10861 gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag
    10921 tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    10981 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    11041 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    11101 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    11161 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    11221 tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct ggtagagcgt
    11281 cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt attttagaga
    11341 tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    11401 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    11461 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    11521 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    11581 ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    11641 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    11701 ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    11761 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    11821 aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    11881 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    11941 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    12001 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    12061 cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg ctgatgacag
    12121 caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc aacaacctgc
    12181 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    12241 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    12301 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    12361 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    12421 tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    12481 cgaacac
//
LOCUS       DAJTRM010000106        12291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRM010000106 DAJTRM010000000
VERSION     DAJTRM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.106"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            696..779
                     /locus_tag="OO322_003332"
     CDS             696..779
                     /locus_tag="OO322_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5908092.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(833..2185)
                     /gene="gorA"
                     /locus_tag="OO322_003333"
     CDS             complement(833..2185)
                     /gene="gorA"
                     /locus_tag="OO322_003333"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5908093.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2257..3099)
                     /gene="rlmJ"
                     /locus_tag="OO322_003334"
     CDS             complement(2257..3099)
                     /gene="rlmJ"
                     /locus_tag="OO322_003334"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5908094.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3302..5344
                     /gene="prlC"
                     /locus_tag="OO322_003335"
     CDS             3302..5344
                     /gene="prlC"
                     /locus_tag="OO322_003335"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5908095.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5352..6104
                     /gene="rsmJ"
                     /locus_tag="OO322_003336"
     CDS             5352..6104
                     /gene="rsmJ"
                     /locus_tag="OO322_003336"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5908096.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6153..7622)
                     /gene="dtpB"
                     /locus_tag="OO322_003337"
                     /pseudo
     CDS             complement(6153..7622)
                     /gene="dtpB"
                     /locus_tag="OO322_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7940..8374)
                     /gene="uspA"
                     /locus_tag="OO322_003338"
     CDS             complement(7940..8374)
                     /gene="uspA"
                     /locus_tag="OO322_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5908097.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8768..9103
                     /gene="uspB"
                     /locus_tag="OO322_003339"
     CDS             8768..9103
                     /gene="uspB"
                     /locus_tag="OO322_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5908098.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9174..10673)
                     /gene="pitA"
                     /locus_tag="OO322_003340"
     CDS             complement(9174..10673)
                     /gene="pitA"
                     /locus_tag="OO322_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5908099.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10905..12104
                     /locus_tag="OO322_003341"
     CDS             10905..12104
                     /locus_tag="OO322_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5908100.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3014 a         3117 c         3293 g         2867 t
ORIGIN      
        1 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
       61 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
      121 gcgtcggttt tttactcttc agcctgagtt ggtggctctg gctggagtga gaatgcccac
      181 atctaacggt tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca
      241 gctggagaag ggacagtttg ctatacatgg tggtgaaaga ggatttatat actttcatcg
      301 ggtcgctgcc gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac ttcgcgtcgg
      361 tttttttact cttcagccca gagttggtgg ctctggctgg agtgagaatg ccatcatcta
      421 acgcatcaat gctaatacca tactgaaact attgcaaggt tatgctggtt atttagccta
      481 attacactgt atgattatcc agttagctct gaggcatttt cactctggca atgcgcataa
      541 acgctttcaa agtcctggtc agaagtacgg gtggtgccgt taactgatgc tctggccgga
      601 gtgagattgc ccacatctaa caatgaggca tgcgccgtga caggcagtgg atgagtaagc
      661 ggatgcattc tcactccatc gcatggagaa aacgggtgat tgataaagca atcatcgttc
      721 ttggggcgtt aattgcgctg ctggaactga tccgctttct gcttcagctt ctgaactgat
      781 agcggaaacg taattaaggg ctaagagcac actactctta gccctttaac atttaacgca
      841 tggtcacgaa ctcttctgcc gccgttgggt gaatggcgac ggtattgtcg aagtcttttt
      901 tggttgcgcc catcttcagc gccaccgcga agccctgcaa catttcgtcc ataccaaagc
      961 caatgccgtg aataccgaca atcttctctt ctggcccaac gcacaccagc ttcatgcggc
     1021 acggctggcg gtgagtggtg acggcggtat acatcgcggt gaaagaggat ttatacactt
     1081 tcacctgatc gtcgccgtac tgctcgcgcg cctgcggttc cgttaaacca acagtaccaa
     1141 tcggcggatg gctgaagacc acggtcggaa tgttgctgta atccagatgc tcatccggct
     1201 tgttattaaa caggcgttca gagagacggc gacccgctgc aactgccacc ggtgtcagtt
     1261 ccactgcacc cgtgttatcg cccaccgcgt aaataccttc aatattggtg ttttgatatt
     1321 tatcgacgac gatatagcct ttttcgttgg ttttaacgcc agcggcttcg aggttgatgt
     1381 tgtcattggc aggctcgcga ccaatcgccc aaatcaggca atccaccgtt tcactgcgac
     1441 catcttccag ctccagcgtc aggctaccat cggcattttt cactaccgct ttcgggatgg
     1501 cgttggtatg caactgtggg ccttcggcgt tcatcacttc gaccagcgtt tcagagatca
     1561 tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtt ttcgcgccga
     1621 ggccgttaat cacgccagcc agctcaacgg cgatgtaacc cgcgccaaca accgccacgc
     1681 gctctggcaa agcaggaagg gcgaagaagc catcggaatc aataccgtat tccacgcctg
     1741 gaatatccgg gtggctcgga cgaccgccgg tggcgatcag aatatgatcg gccgtgatgg
     1801 tttcgccgtt aacttccagc gttttggcat caacgaagcg ggcaaagcct ttgattacat
     1861 caacgttatt tttaccgagc acgttttcat aggaagtatg aatacggtcg atataggcgg
     1921 tacggctggc gatcaacgtt tcccagttga acttgttgat ggtggtatcg aaaccataat
     1981 cagggccgta catatggatc gcttcacgga tttgcgccgc gtgccacatc acttttttcg
     2041 gcacgcagcc gacgttcacg caggtgccgc ccagctcttt ggcttcaatc agtgcgcatt
     2101 tctggccgta catagccgcg cggttgatgg aggcgatacc gccgctgccg ccgccgatgg
     2161 cgatgtaatc atagtgttta gtcatgacat attgtcctta tcgttgatta ccgcgattgt
     2221 agcgcgagac gtaataggtg ccagcaatgg ctgcaattac tccggcacga tccagcttac
     2281 ggtggcgtgc ccggtacctg ccggaaccag tttgctgtgc agccacgaca gcacgttatt
     2341 catctgctgt tccagcttcc acggcgggtt aatcacaatc atgccggaag cggtcatgcc
     2401 acggcgatcg ctgtctggca gcaccgccag ttcaatttgc agaattttgc gaatgccggt
     2461 cgcttccagg tcgtggatca tgcgcttaat ttgctggcgc agcaccaccg gataccacaa
     2521 tgcgtaagtg ccagtggcga aacgtttgta accttctgct atcccgctga ccaccgcctg
     2581 atagtcggtt ttcatttcat acggcgggtc gataaggatt aaaccacggc gagaaaccgg
     2641 cggcagtttg gccttaagtt gctggaaacc gtcggctttt tcgacgcgtg cacggctgtc
     2701 tttctgaaat ccagaacgca gcaacgggta atcgctcggg tgcagttcgg tcagttgcag
     2761 gctgtcttgt tcgcgcagta gctggcgagc aatcaacggc gaaccggggt agtaacgcaa
     2821 ctggccgcta cggttgaagt gttttaccac attgatgtac gcctccagtt ctgcgggcaa
     2881 atcgtcctgc tgccagatac gggcgatacc ttcgagatat tcgccggtac gctcggcatg
     2941 ttcgctacct aactgataac gcccggcccc tgcgtgggtg tcgagataga gaaacggttt
     3001 atctttctct ttcagcgact cgatgatcag gctctgaacg gtatgtttaa ggacgtcggc
     3061 gtggttgcca gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg ttccggtaag
     3121 taaaaattgg cccgtattcg gggcgcacaa aagcctatca ggacagtata ccgaaaagag
     3181 gccgccgccg cgaaagcgta acgtttctca ttgaaattca ctacacttaa ccccatgcta
     3241 cacacattat gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca ggactgcgct
     3301 aatgacgaat ccgttactga ctccctttga attgcctccg ttttctaaaa ttctcccgga
     3361 acatgtcgtt ccagccgtga ctaaggcatt gaacgactgc cgcgaaaatg tggagcgcgt
     3421 agtagcgcaa ggggcaccgt acacctggga aaatctctgc cagccgttgg cggaagtgga
     3481 cgatgttttg gggcgtatct tctccccggt cagccacctg aactcggtga aaaatagccc
     3541 ggaactgcgt gaagcgtacg aacaaaccct gccgctgctg tcggaataca gcacctgggt
     3601 agggcaacat gaagggctgt ataaagcgta ccgcgacctg cgcgatggcg atcattacgc
     3661 cacgctgaac acggcgcaga agaaagcggt tgataacgcc ctgcgtgatt ttgaactctc
     3721 tggcataggt ctgccgaaag agaaacagca gcgttacggc gaaattgcta cccgtctttc
     3781 tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg acgatgggct ggaccaaact
     3841 cgttaccgac gaagcggggc tggcggggat gccagaaagc gcgctggctg cggcaaaagc
     3901 ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg ctggatatcc caagctacct
     3961 gccggtaatg acctactgcg acaaccaggc tctgcgcgaa gagatgtatc gcgcttacag
     4021 cacccgcgcc tccgatcaag gcccgaatgc cggtaagtgg gataacagca aggtgatgga
     4081 agagatcctc gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa actacgcctt
     4141 taaatccctt gccactaaaa tggcagaaaa cccgcagcag gtgctggatt tcttaaccga
     4201 tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg gagcaactgc gtgccttcgc
     4261 taaagccgaa tttggtgtcg atgagttgca gccgtgggat atcgcttact acagcgaaaa
     4321 acagaaacag cacctctaca gcatcagcga cgagcagctg cgtccgtact tcccggaaaa
     4381 caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt tacggcatca ccgctaaaga
     4441 gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa
     4501 taacgaactg cgcggcagct tctacctcga tctgtatgcc cgtgaaaaca agcgcggcgg
     4561 ggcgtggatg gatggctgcg taggccagat gcgtaaagct gatggttctc tgcaaaaacc
     4621 ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac
     4681 tcacgacgaa gtgatcaccc tgttccacga gttcggtcac ggcctgcacc atatgctgac
     4741 ccgcatcgaa accgctggtg tttccggtat cagcggtgtg ccgtgggatg cggtcgaact
     4801 gccgagtcag tttatggaaa actggtgctg ggagccggag gcgctggcgt ttatctctgg
     4861 tcactatgaa accggcgaac cgctgccgaa agagttgctg gataaaatgc tggcggcgaa
     4921 gaactaccag gcggcgctgt ttattctgcg tcagctggag ttcggcctgt ttgatttccg
     4981 ccttcatgcc gagttccgcc cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat
     5041 caagaaactg gttgccgtgg tgccatctcc gtcctggggc cgtttcccgc acgctttcag
     5101 ccatattttc gccggtggtt atgcggcagg ttactacagc tacctgtggg ctgacgtact
     5161 ggcggcagat gctttctcgc gctttgagga agagggcatt ttcaaccgtg aaaccgggca
     5221 gtcgttcctc gacaacattc tgagccgtgg cggttcagaa gagccgatgg atctgttcaa
     5281 acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg gagcattacg gcattaaggg
     5341 ctgatcattc agtgaaaatc tgcttaattg atgaaacagg cgccggagac ggcgccttat
     5401 ctgttctggc ggcccgctgg gggctggagc acgatgaaga caacctgatg gcgctggtgt
     5461 taacgccgga acatctggaa ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg
     5521 ttgattttgt tggcggagcg atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg
     5581 cggtggcgaa agcggtgggc attaaaggcg attatttgcc ggatgtggtg gacgccaccg
     5641 cagggctggg gcgcgatgcc tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg
     5701 agcgtaatcc agtggttgcc gcgctactcg acgacggcct ggcgcgtggt tatgcggatg
     5761 cggaaatcgg cggctggttg caggagcggt tgcagttaat tcacgcctcc agcctgacgg
     5821 cgctaaccga tattacccca cgcccgcagg tggtctatct cgatccgatg ttcccgcata
     5881 agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt tcagtcgctg gtggggccgg
     5941 atcttgatgc cgatggatta ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg
     6001 taaagcgccc ggactacgcg ccaccgctgg cgaatgtcgc cacgccaaac gcggttgtga
     6061 ctaaagggca tcgctttgat atctatgcag gtacgccagt gtaaaagtaa agcagggtag
     6121 cgataacgct accctgcttt ctgcaagaat aattaatggc tttccggcgt cgcaatcatg
     6181 cgtttcagcc acggcaccat caacagcatc actactgcaa cgcccagcgt gaccagacca
     6241 atcttaccaa acacgttggt atagacgggc aacgtctcaa gcggatcggt aatgttgtcc
     6301 ggcaccgcgg taaatgttgc cacatagccg cccagcaaga acgcggcagc ctgcgtcagg
     6361 aaccacatcc cgagaataaa gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc
     6421 aggccaaggg cgctaataaa caattcaccc aggctctaga ataaatacac cagcacgata
     6481 aaccatggcg atgtcagccc ctggtcatcc gcaaaccaca ttcccgcagc agcagccgtc
     6541 aaaaagccca gtgagcacat aaacatgctg agagtaaatt tcatcggcat cgagaggtct
     6601 ttgcctttgt tacccagatg cgtgtaaatg cctgccagta ttgggctggc gagtaccacc
     6661 cagaacgggt taagcgcctg gaagctgacc gggttgatgg aaaaaccgag aatttcatga
     6721 tgcacgttgt tgatggcaaa gaagttcagc gatgttggca tctgggcgta gagaatgtaa
     6781 aacaccaccg cttcgagcat caggacaaag gcgacaaaca ttttattgcg cccggtttta
     6841 tccagcttga atgcctgacg aaagaagatg atagtgacga cgatggagag aacaatcagc
     6901 accagattgg cgacttctac gttgtgcatc agccatgcac atacgaagat catcaccacg
     6961 ctgccaagta atacgtacaa cagtttgctg aagctcatcg gcttgaagtc gggttcagaa
     7021 ccaatgtctt tcaccattcc acgacaggcg atgtaaacca gtaatgcgat aattaacccc
     7081 gccccgcaca ggttgtaggt gactgaataa ccgaatctat cagcgatcac aggggccagc
     7141 gataacgcta tcaacgagcc gatattgatc gacatataga acagggtgaa tgcgccatca
     7201 agccgcggat ctttcggcgg atagcacttc gaaagcaagc tggctgggtt agctttaaac
     7261 aggccgttac cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc
     7321 gacatgccgg tcatgaagta gccaatcgcc agcacaagtg ctccaagaac aatggtgcgt
     7381 ttggtcccca acaggtggtc gccgacatag ccgccaatgg aaatgaggcc atagaccagt
     7441 gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg
     7501 aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga atcgctccca taactcgaca
     7561 aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt tgttgtatta
     7621 atatttatta accttccaat accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg
     7681 aaaaagttta catctgtcag agattcttaa tattgatgcg gtgaatagat taatacacta
     7741 ttacaacaga aaataaccag ctgatcacac taatgatgga aaatatgatg gtgttaaatt
     7801 ttgtattggc aggagatttt gattgcgtac gcagatcgta ggccagataa ggggtttacg
     7861 ctgcatcagg taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga
     7921 acacgtcgtg gagggatgat tattcttctt cgtcgcgtag cggaacaatc agcatatcaa
     7981 cgtgaacggt gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt
     8041 ggtgaccaca aaccaccaga tccatatcgt atttcttgat tgcatcgacc agaacctggc
     8101 ctaggtcgcc gctgccgctc agggtttcag tgattggata gcctgcatta gtggaaagct
     8161 cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat
     8221 caataagccc ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg
     8281 cattgtaggg gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga
     8341 gatcgactgc gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc
     8401 gatgactggc cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt
     8461 gcgcggggct aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac
     8521 ccggatgatc cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat
     8581 attatattga tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta
     8641 tagagccgct cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg
     8701 attggcagcg tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg
     8761 aggaggtatg ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa
     8821 catggcgcgc tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc
     8881 attgctctat caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa
     8941 acaggtgcgt ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga
     9001 gtttattcgc cgctgtgagc gggtgcgtcg gcagtttatt ctgactagcg cattgtgtgg
     9061 tctggtggtg gtcagcctga ttgcattgat gatttggcat taagcacttc agcaataata
     9121 acgtataaaa aaagcgggcc agtaaactga cccgctcgtt ttggtgcgta cgattacagg
     9181 aactgcaagg agaaccagta cagcccgccg gaaagtagta ccgcagccgg aagggtaaac
     9241 acccaggcca tcagaatact ggttacggtt ttacgctgta agccgccgcc atctaccacc
     9301 atcgtccccg cgacagaaga ggagagtacg tgagtggtgg aaaccggcat cccggtataa
     9361 ctcgccaggc cgattgacac tgccgccgtc atctgggcag acatcccctg agcgtaggtc
     9421 atgcctttct taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc
     9481 gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc acaccggcgc atactcgatg
     9541 gtgctaagca tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacacca
     9601 ggcatcttca ccactttgtc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg
     9661 ctacgttgat caagcgacag cttgtcgtag ctttccacat cggtggtcaa catacctttc
     9721 aggcggttga gcgcgttaat ggtattcgac ggatggcagt ggaactccgc aggttgcgtt
     9781 gcgccagctt ccggagccgg tactaactga tcagcaccgg tagcctgttt gagcagcgca
     9841 ggatgctgct caaagtaagc ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg
     9901 tagccagtgg cattcatgtt caccacgaag cctgctggcg cgacgccaat caataccaac
     9961 ataaccagac caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc cacgccgata
    10021 gcggaaagga tcagcgcgat acgcgtccag aacggcggct ttttcttgcc gtctttcttt
    10081 tcacgctccg ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga
    10141 cgcagcaaga aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga
    10201 gaaccgaaaa tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc
    10261 atcaacgcat tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat
    10321 gcaggtaaac caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag
    10381 aacaccatgg caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata
    10441 tgcacaatgg cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaacacc
    10501 gccgccataa ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg
    10561 gcgttggctg tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca
    10621 agcaataata acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt
    10681 tacgccattt tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa
    10741 gtgaaatata gacttttttt gatttgcctc ctgtatggat ttcactcaaa aaataattat
    10801 cttatataat tcaggcaaat acttcctttt agtaatattg atgctggtgc gaccactgag
    10861 gaatctttac aattcacgcc cgttttttct aagaggagcg caacgtggaa aggtttgatg
    10921 ccattattat aggcgctggt gcggcgggta tgttctgttc tgcgctggca ggtcaggtag
    10981 gacgccgggt tctgctgatc gataatggta aaatactagg gcgcaaaatc cttatgtctg
    11041 gcggtgggcg ctgcaacttt accaaccttt atgtcgaacc aggcgcttat ctgagccaga
    11101 atccgcattt ttgtaagtct gcactcgcac gttttaccca gtgggatttc attgatctgg
    11161 tcaataaaca cggtatcgcc tggcacgaga aaacgttagg gcaactcttc tgcgatgact
    11221 ccgcgcagca gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtaaccttca
    11281 gattgcgtag cgaagtgctg agtgtggcga aggatgaaac aggcttcacg cttgatctga
    11341 acggcatgac tgtcggttgc gaaaagctgg tcatcgcgac cggtgggctg tcaatgccgg
    11401 ggctgggcgc atcgccgttt ggttataaga ttgccgaaca atttggcctc aacgtgctgc
    11461 cgacccgcgc gggtctggtg ccattcactc tgcataaacc gttgctcgaa gagttacagg
    11521 tgctggcggg cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgtg
    11581 agaacttact cttcacccac cgcggcttat ctggaccggc ggtgttgcag atttcaagct
    11641 actggcaacc gggtgaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct
    11701 tcctgaatga gcagcgtaac gcacatccga atcaaagcct gaaaaacaca ctggcggttc
    11761 atctaccgaa gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc
    11821 taaaacagct caacgtgtgt gaccaacagg cactgattag cacattgacc gactggcgcg
    11881 tacaacccaa cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca
    11941 ccaacgaact ttcttcacgg acgatggaag cgcgcaaagt gcctgggctg tacttcatcg
    12001 gtgaagtgat ggacgttacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga
    12061 gtgcatgggc ttgtgcgcag gatttggcgt caattgtgaa ataaccatga cgttttcccg
    12121 tcagccgttt gtatatgtcg ttattaagtc attttggtac ggttctgcat ccgacattct
    12181 ggtgttaact cgctgttttg ttagcgaatt aacgctattg ccgttgtggt tattttatag
    12241 tgtatttatt ctcagagaat atatattact gaaataaaaa acacaaagaa t
//
LOCUS       DAJTRM010000107        12062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRM010000107 DAJTRM010000000
VERSION     DAJTRM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.107"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO322_003342"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO322_003342"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO322_003343"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO322_003343"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5908101.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO322_003344"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO322_003344"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5908102.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO322_003345"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO322_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO322_003346"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO322_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5908103.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO322_003347"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO322_003347"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5908104.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO322_003348"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO322_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5908105.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO322_003349"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO322_003349"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5908106.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO322_003350"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO322_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5908107.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO322_003351"
     CDS             9553..10332
                     /locus_tag="OO322_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5908108.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO322_003352"
     CDS             10322..11131
                     /locus_tag="OO322_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5908109.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO322_003353"
     CDS             11218..11652
                     /locus_tag="OO322_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5908110.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="OO322_003354"
     CDS             11649..>12062
                     /locus_tag="OO322_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5908111.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3252 c         3077 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAJTRM010000108        11744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRM010000108 DAJTRM010000000
VERSION     DAJTRM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.108"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..250)
                     /locus_tag="OO322_003355"
     CDS             complement(<1..250)
                     /locus_tag="OO322_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908112.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQD"
     gene            complement(281..973)
                     /gene="fimC"
                     /locus_tag="OO322_003356"
     CDS             complement(281..973)
                     /gene="fimC"
                     /locus_tag="OO322_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5908113.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1193..1735)
                     /gene="fimA"
                     /locus_tag="OO322_003357"
     CDS             complement(1193..1735)
                     /gene="fimA"
                     /locus_tag="OO322_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5908114.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2216..3082
                     /gene="folD"
                     /locus_tag="OO322_003358"
     CDS             2216..3082
                     /gene="folD"
                     /locus_tag="OO322_003358"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5908115.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3084..3296
                     /gene="ybcJ"
                     /locus_tag="OO322_003359"
     CDS             3084..3296
                     /gene="ybcJ"
                     /locus_tag="OO322_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5908116.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3404..3913
                     /locus_tag="OO322_003360"
     CDS             3404..3913
                     /locus_tag="OO322_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5908117.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(3949..5334)
                     /gene="cysS"
                     /locus_tag="OO322_003361"
     CDS             complement(3949..5334)
                     /gene="cysS"
                     /locus_tag="OO322_003361"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5908118.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5508..6002
                     /gene="ppiB"
                     /locus_tag="OO322_003362"
     CDS             5508..6002
                     /gene="ppiB"
                     /locus_tag="OO322_003362"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5908119.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6005..6727
                     /gene="lpxH"
                     /locus_tag="OO322_003363"
     CDS             6005..6727
                     /gene="lpxH"
                     /locus_tag="OO322_003363"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5908120.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6845..7354
                     /gene="purE"
                     /locus_tag="OO322_003364"
     CDS             6845..7354
                     /gene="purE"
                     /locus_tag="OO322_003364"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5908121.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7351..8418
                     /gene="purK"
                     /locus_tag="OO322_003365"
     CDS             7351..8418
                     /gene="purK"
                     /locus_tag="OO322_003365"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5908122.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8555..9448)
                     /locus_tag="OO322_003366"
     CDS             complement(8555..9448)
                     /locus_tag="OO322_003366"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5908123.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9445..10092)
                     /locus_tag="OO322_003367"
                     /pseudo
     CDS             complement(9445..10092)
                     /locus_tag="OO322_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10095..11327
                     /gene="allC"
                     /locus_tag="OO322_003368"
     CDS             10095..11327
                     /gene="allC"
                     /locus_tag="OO322_003368"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5908124.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11338..>11744
                     /locus_tag="OO322_003369"
     CDS             11338..>11744
                     /locus_tag="OO322_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR5908125.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRR"
BASE COUNT         2844 a         3058 c         3006 g         2836 t
ORIGIN      
        1 tatcctgtga accaatgaac tcatcattac gccagagatc cacccgatac acgcccgcag
       61 gttgatgatt acctctctca aagcgcgata aatcagcaac ggatgtgcca ttctctaata
      121 aaaatgccgg gttgaaataa ctttccgcat agcttaaaaa ggagggtaaa atggcaagag
      181 atgaataaca aattccggcc agacaccagg tatacctctg cggaatatcc gtagtagtgg
      241 gtattttcat aattagctct gatattgtca gatgattcgg ttagtttaag ttcacttctc
      301 tgaccggggt tactgaacca taatcattaa cggtctgaaa tttcagcttt ccctgcactc
      361 ctgagggtaa gggaatttgt gaactaactc tgggtgccgc catagcattc cccaactttt
      421 ggctgcccac ttgcaggtta acgagggtga cataaaaagg tgatggatta tgaacattga
      481 tatagttacc ggatcgcgaa aacttgagtg tgtccggcgc ttctgcgggt aattcttgta
      541 attgaattgg acggagaaat aatttcatgc gcgataaaat cgccagttgc aaaacattcc
      601 tgccgttcaa tgcattttta tctaccgaag ggatcgtctt aacattcatc cagaacagag
      661 actcacgatc tgccgccagc ggtggaccgg tgtaaataat acgcaaagta ttttcgcttt
      721 tgggttcact aacaaatagt ggcggtgtaa tgatgaatga cttttctttt acaccgctgc
      781 tgttttcaat ccacgaattg accagaaaga gctcattggt atggctattt ttaatccata
      841 ccgcagtctg tttagcatca gcgggataaa taatacgcgt ggcacctaat gcgatccctc
      901 cggcagcatg ggcgctggcc gaaatgatta aataaaatat catgagcatc aataacttta
      961 ttttagtcat catagttaat acgccttgtg tattcctgac ggcaaaaata aggagtataa
     1021 agaaatgatt gctttggctt cttacaacac atcattccct tttcatttaa taaagcggca
     1081 tgatacaaag tgaacgtggt ttcgatttac attgccacca taccgcttca gacaaccggt
     1141 acattacttt gttataagac gtgatatata attcaaaaca acgtggtttt gattattcgt
     1201 atctcataat aaaagtcgcg tcagcatttg cttgccctgc tgatgcactt gtaccggtgc
     1261 ctttataacg tgcagaaaaa tgaagaacgt tggtcccggg gatcaagttc tggttggttg
     1321 agaagctatt cccatcaggt ttgagaattg cggatgtatt atcaagtatt tcaataccga
     1381 cacccgttgc tgttgttgta tttgtactgg atacaatggc caataaatta tcattgattg
     1441 catcagcctg accagaaaat gcaacggcag cattagcggc aacaacagga tcgcagtcgt
     1501 tcaactgaat ggtgaatgga attaatgcag aggtattacc aacagcattg aaaatatcgg
     1561 tacgatattg accgagtgtg acaacctggt ctgccgagtc agtattcact gaacaggcag
     1621 cattcaccag ttcgccttca aagtgaattg taccgccatc tacaacggca gcataactac
     1681 ccgtagcggc gaatagtgcg gcagccagcg cagacgaaat aaatcttaat ttcatatata
     1741 ttccttcaat ctcatttatc gactccacat ccgtatataa ccgattactt tatttaagac
     1801 actgatagta gtaaaattcc tttttatcct ctaagaatgt cttaattgaa aatatgcact
     1861 ctattctaaa aaatagagag ccccgttaga tgaatacttc cgcgcaaaat atattcacca
     1921 caaatataga cctgaagcgg taaattacca ggctgaaaat tctttttata ttgtcaggta
     1981 tttcttaaat tatcttaatc cttaaacaag gaaataaatc agttccagat ttacaacgcc
     2041 atcatgtacg aaaaatgaag ctttcaatct cagcgacctc agcgacggtg cgcctaacct
     2101 tcgcaagagg tcgcttcacg cgataaatct gaaacgaaac ctgacagcgc gccccgcttc
     2161 tgacaaaata gacgcatccc cttcgaccta cgtaacagat ggaatcctct ctctgatggc
     2221 agcaaagatt attgacggta aaacgattgc gcagcaggtg cgctctgaag ttgctcaaaa
     2281 agttcaggct cgtattgcag ccggactgcg ggcaccagga ctggccgttg tgctggtggg
     2341 tagtaaccct gcatcgcaaa tttatgtcgc aagcaaacgc aaggcttgtg aagaagtcgg
     2401 gttcgtctcc cgctcttatg acctcccgga aaccaccagc gaagcggagc tgctggagct
     2461 tatcgatgcg ctgaatgccg acaacaccat cgatggcatt ctggttcaac tgccgttacc
     2521 ggcgggtatt gataacgtca aagtgctgga acgtattcat ccggacaaag acgtggacgg
     2581 tttccatcct tacaacgtcg gtcgtctgtg ccagcgcgcg ccgcgtctgc gtccctgcac
     2641 cccgcgcggt atcgtcacgc tgcttgagcg ttacaacatt gataccttcg gcctcaacgc
     2701 cgtggtgatt ggcgcatcga atatcgtcgg tcgcccaatg agcatggaac tgttgctcgc
     2761 aggttgcacc accaccgtga ctcaccgctt cactaaaaat ctgcgtcatc acgtagaaaa
     2821 tgccgatctg ttgatcgttg ccgttggcaa gccaggcttt attcccggtg actggatcaa
     2881 agaaggcgca attgtgattg atgtcggcat caaccgtctg gaaaatggca aagttgtggg
     2941 cgacgtcgtg tttgaagacg cggctaaacg cgcctcatac attacgcctg ttcccggcgg
     3001 cgttggcccg atgacggttg ccacgctgat tgaaaacacg ctacaggcgt gcgttgaata
     3061 tcatgatcca cagggtgagt aacatggcga cattttcttt aggtaaacat ccgcacgttg
     3121 agctgtgcga cttgctgaaa ctggaaggct ggagcgaaag cggcgcgcag gcgaaaatcg
     3181 cgattgccga aggccaggtg aaagtcgacg gtgcggttga aacgcgcaaa cgctgcaaaa
     3241 tcgtcgccgg tcagacagtg agttttgcag gacacagcgt acaggttgtt gcctgatccc
     3301 tttcccggcc tgtatcaaca ggccggttct cttttcagac ttttgccctc tacgccccgt
     3361 tcatccatgc tgatagcatc gacattttgc cgaagcgacc atcatgccaa ccgttattac
     3421 gcacgccgcc gttccccttt gcattggttt agggttgggt tcgaaaggtc tgttatttgc
     3481 cggaatcatc ctggcgatgc tgcccgatgc cgacgtattg tcgtttaaat ttggcgttgc
     3541 ttacggcaat gtttttggtc atcgcggttt tacccattcg ctggtgcttg cgtttgttgt
     3601 cccgctctta tgtgtgctga ttggacgtcg atggttcagg gctgggctga ttcgctgctg
     3661 gctgttttta accgtctcgt tgctatcgca cagcttactg gattcggtaa ccactggcgg
     3721 taagggcgtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc cctggcaggt
     3781 gattaaagtc gcgccgtttg cattgtctcg ttacaccaca gcgtacgggc atcaggtgat
     3841 tatttccgaa ttgatgtggg tgtggttgcc ggggatgcta ctgatgggaa tgttgtggtg
     3901 gcgcagacga taaccggatg cgaaaactcg catccggcaa ttgcgcaatt acttacgacg
     3961 ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcgttaa gacggtcacg
     4021 tgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct gttgaattaa
     4081 cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca ggaacgcttc
     4141 cggttcttgc tccagcaggc ccaatacagc ggaaagttta cgcaggtgag atgccattgc
     4201 attcgccgct gccatatctt ctgctttcag acggttcact tcacgcgcca tatcaaacag
     4261 cacggaatag gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa agcgcgcttc
     4321 aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg cagtgtagag
     4381 gcgctccagc gccgcacgcg cctgcttcag gttctcttcg ctgtagttca gctggctgcg
     4441 atagtggccc gacatcagga agtaacgcac ggtttccgcg tcgtagtatt tcagcacatc
     4501 gcgcacggta aagaagttac ccagcgattt ggacatcttc tcgcggtcaa ccatcaccat
     4561 tccggagtgc atccagtagt tcacatactg accatcatgg gcacaggtgg actgcgcgat
     4621 ttcgttctcg tggtgcggga acatcaggtc tgaaccgccg ccgtggatat caaagtggtt
     4681 acccagctgc ttgcagttca tggccgaaca ttcaatgtgc cagccaggac gacccgcgcc
     4741 ccacggagac ggccagctcg gttcgccctc tttcgacatc ttccacagaa cgaagtccat
     4801 ccggttgcgt ttgtcatcaa ccacgtcaac gcgcgcgcca gcctgcagct ggtcgagatc
     4861 ctgacgcgac agcacgccat aagttggatc ggtcgggacg tcgaacatca cgtcgctgtt
     4921 gtccgccaca taagcgtgac ctttggcgat cagttgttca gtgagttcaa taatttctgc
     4981 gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag catcaaaatc
     5041 tttgtgcatt tcggcgatca tgcgatccac cagcgccaca aagctttcgc cattttcatt
     5101 ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg cgcacatact tcagtttata
     5161 gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac gcccgtgacc
     5221 gatatgacag agatcgtaaa cggtgattcc acacacgtac atgccgactt ccccggcgtg
     5281 aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta gcatcgaaga
     5341 ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt cccgcgcgaa
     5401 tcagcataca ttgcgtgatg atccgatcgt gcggttatgc tataacacca ccctatatat
     5461 gacccgaact gggttgaagc accaatcaaa cggaacagga tgcaaaaatg gttactttcc
     5521 acaccaatca cggcgatatt gtcatcaaaa cttttgacga taaagcacct gaaacagtta
     5581 aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc caccgtgtta
     5641 tcaacggctt tatgattcag ggcggcggtt ttgaaccggg catgaaacaa aaagccacca
     5701 aagaaccgat caaaaacgaa gccaacaacg gcctgaaaaa tacccgtggt acgctggcaa
     5761 tggcacgtac tcaggctccg cactctgcaa ctgcacagtt cttcatcaac gtggttgata
     5821 acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc gtgtttgctg
     5881 aagtggttga cggcatggac gtggtagaca aaatcaaagg tgttgcaacc ggtcgtaacg
     5941 gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc gttagcgagt
     6001 aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac cggcgatcac
     6061 cgccggtttt ctgcgttttt tagcggggga agcccgcaag gccgacgcgc tgtatattct
     6121 tggcgatctg tttgaagcat ggattggcga cgacgatccc aacccactcc atcgccagat
     6181 ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc tgttatttca ttcatggcaa
     6241 ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagc ggcatgacat tattgccgga
     6301 agaaaaggtg ctcgaacttt atggtcgccg ggtgttgatt atgcatggcg acacgctgtg
     6361 caccgatgac gcgggttatc aggcttttcg cgccaaagtc cacaaaccct ggctgcaaat
     6421 gctattcctc gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa tgcgcgcgaa
     6481 cagcaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc aaaacgctgt
     6541 ggtcagtgcg atggaaaaac atcaggtgca atggctaatc cacgggcata cccatcgccc
     6601 ggcggtgcat gaacttatcg ccaaccagca acctgctttt cgcgtggtac tgggtgcctg
     6661 gcatacggaa ggttcgatgg tgaaagtcac ggctgatgac gttgagctga ttcattttcc
     6721 gttttaaaaa acccacaatt ttgctgattt cacagccacg caaccgtttt ccttgctctc
     6781 tttccgtgct attctctgtg ccctctaaag ccgagagttg tgcaccacag gagttttaag
     6841 acgcatgtct tcccgcaata atccggcgcg tgtcgccatc gtgatggggt ccaaaagcga
     6901 ctgggctacc atgcagttcg ccgtcgaaat cttcgaaatc ctgaatgtcc cgcaccacgt
     6961 tgaagtggtt tctgctcacc gcacccccga taaactgttc agcttcgccg aaagcgccga
     7021 agagaacggt tatcaggtga ttattgcggg cgcaggcggc gcagcgcacc tgccaggcat
     7081 gattgccgcc aaaacgctgg tgccggtgct gggcgtgcca gtacagagcg ccgcactgag
     7141 cggtgtcgat agcctctact ccatcgtaca aatgccgcgc ggcattccgg tgggtacgct
     7201 ggcgattggt aaagctggcg cggcaaacgc ggcattactg gcagcacaaa ttctcgcgac
     7261 tcacgataaa gagctacacc agcgtctgaa tggctggcgc aaagcccaga ccgacgaagt
     7321 gctggaaaac ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct cggtaacggg
     7381 cagttaggcc gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt ctggccggtc
     7441 gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac cgctgagatc
     7501 gaacgctggc cggaaaccgc attaacccgc gagctggcgc gtcatccggc ctttgtgaac
     7561 cgcgatgtgt tcccgattat tgctgaccgt ctgactcaga aacagctttt cgataagctc
     7621 cacctgccga ccgcgccgtg gcagttactt gccgatcgca gcgagtggcc tgcggtgttt
     7681 gatcgtttag gtgaactggc gattgttaag cgtcgcactg gtggctatga cggtcgcggt
     7741 caatggagtt tacgcgccaa tgaaaccgaa cagttaccgg cagagtgtta cggtgaatgt
     7801 attgtcgagc agggcattaa cttctctggt gaagtatcgc tggttggcgc gcgcggcttt
     7861 gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg tattttgcgc
     7921 accagcgtcg cttttccgca ggccaacgca cagcagcagg cgcaagccga agagatgctg
     7981 tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg ttttgtcacc
     8041 ccgcaaggtc tgctgatcaa cgaactggct ccgcgtgtgc ataacagcgg tcactggaca
     8101 caaaacggtg ccagcatcag ccagtttgag ctgcatctgc gggcgattac cgatctgccg
     8161 ttaccgcaac cagtagtgaa taatccgtcg gtgatgatca atctgattgg tagcgatgtg
     8221 aattatgact ggctgaaatt gccgctggtg catctgcact ggtacgacaa agaagtccgt
     8281 ccggggcgta aagtggggca tctgaatttg accgacagcg acacatcgcg tctgactgcg
     8341 acgctggaag ccttaatccc gctgctgccg ccagagtatg ccagcggcgt gatttgggcg
     8401 cagagtaagt tcagttaagc aggtgctcat ttgattgccg gatgcggcgt aaacgcctta
     8461 tccggcttac cgctccggta cccattgtag gcctgataag acgcgtcaag cgtcgcatca
     8521 ggcatggtgc accgattgcc ggatgctctc atcactacag cgaaatacag gtccccgctt
     8581 cgcccgccag cgtctcttca attcgcgata acgccccaat ccacgcgggt ttactacggc
     8641 ttctgacata accactcacc gccgttacct tcggccccat cgaaccatcg gctttggcaa
     8701 atggcgctaa ctcatccggt gtggcatggc gaatggcacg ttgctgcggc gttccccagt
     8761 tttcatatac cgcatcagca tcggtgagga tcaccagtcc atcggcatta atctgctcgg
     8821 cgagcaacgc agcggcgaga tctttatcaa tcaccgcttc actccctgcc ccatcttccg
     8881 tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg agcaacaact
     8941 cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc cggcgcaaat
     9001 atttaccatc acgtttcatc tgccagccgt aagccgcttc cagtgcctct tgttcttctg
     9061 gctgataaac cggaccaata aatttctctg gctgcaaaaa cgccggatca tcaggcgaaa
     9121 cctcaatgcg cgtcagcacc gtcgtaacag gcggcatctg cggctgtgcg ctcaagctct
     9181 gcgccagcat atagccaatc atcccctggc tttccgccac cagtacatcc agcggatacg
     9241 gttctacctc tttccacgcc agattctgta acgccagcag ccccacctgc ggcccgttgc
     9301 cgtgaacaat cgccagccga taagaacggg ccaggcgtgc cagcgcgggt acagcactgg
     9361 cgatattgcg atattgattt tccgccgtca gcgcctcacc gcgctggagt aaggcgttgc
     9421 ccccaagagc tacaaccagt gttttcatgg ttttccttgt aataattgtt ggccaagcca
     9481 gaaccccagc agcgtatctg cccctgacgt atgccccagc gccagcagcg aatcaatcgc
     9541 aacagcggta cgtttcgggc aactcagagc atggacaaag tgcaggagtg gcgaggcgaa
     9601 atatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa cgagttgaag
     9661 attgtcggaa caggcaaaaa acgggcgacc ggcgcgcgca tctaaagcgc cataatacca
     9721 ggccgccagc agcattccgc tcaacgtgtc atcatgactt ggcgttaatc cggggccttt
     9781 acccagccag tgccgccagt cggtcttaac gccattgagc gcggcctgaa aacagtgacg
     9841 aaactggcgt aattcagcgg gcagcggatc gcttgccgcc atcgccagtg gcccgaaaag
     9901 tccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgaaggcgg
     9961 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
    10021 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
    10081 ttgcgtgcgg cgaaattaca cattcccgtc aagctataga agaaacgctg ccctggcttt
    10141 cctcttttgg cgctgaccca acgggtggga tgacccgttt actttattcg ccggaatggc
    10201 tggaaaccca gcagcaattt aaaaaaagaa tggcagcaag tgggctggaa acacgtttcg
    10261 atgaagtggg gaatttatac ggtcgcctga gtggcaccga atatccacag gaagtggttc
    10321 tgagcggttc gcatatcgat accgtggtta acggcggtaa cctcgacggg caattcggcg
    10381 cgctggcggc gtggctggca attgactggt tgaaaacgca atacggcgca ccgttacgta
    10441 cggtcgaagt ggtggcgatg gcagaagaag aaggcagccg cttcccgtat gtcttctggg
    10501 gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt gcggaatatc tgtgatgcca
    10561 aaggaaatag ttttgccgat gcgatgaagg cttgcggatt tactctgccg aacgccccac
    10621 taactccgcg tcaggctata aaagcctttg ttgaactgca tattgaacag ggctgtgtgc
    10681 tggaaagtaa tgggcaatca attggcgtgg tgaatgcaat tgtcgggcag cgtcgctata
    10741 cggtgacgct gaacggcgaa tcaaaccatg caggcaccac gccgatgggt tatcgtcgtg
    10801 atacggttta cgctttcagt cgcatttgcc atcagtcgat cgaaaaagcg aaaaagatgg
    10861 gcgatccgct ggttctgacc tttggcaaag tagagccgcg cccgaatacg gtaaatgtgg
    10921 tgccgggtaa aaccacgttc accattgatt gtcgtcatac cgacgctgcc gtgctgcgcg
    10981 atttcaccca acagttagaa aacgacatgc gggcgatttg cgatgaaatg gacattggta
    11041 ttgatatcga tttatggatg gacgaagaac ccgtgccgat gaataaggag ctggtcgcca
    11101 ccctgacaga attgtgtgaa agagaaaaac tgaattaccg ggtgatgcac agtggtgccg
    11161 ggcacgacgc gcaaattttc gcgcctcgcg taccaacctg catgattttt atccccagca
    11221 tcaacgggat cagccataac ccggcggaac gcaccaatat tactgacctt gccgaagggg
    11281 tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca ggaataagga gtcataaatg
    11341 ggatatttaa ataacgtcac cggttaccgc gaagatttac tggctaaccg tgcgattgtt
    11401 aaacacggta atttcgcact gttaaccccg gatggtctga taaaaaatat tattccggac
    11461 tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc tgggtgcctc ttttgttgat
    11521 tatctggtca cactgcatca aaacggtggc aaccaacagg gcttcggtgg cgaaggcatt
    11581 gaaacgttcc tgtatgtgat ctctggaaat atcactgcca aagccgaagg caaaacattt
    11641 gccttaagcg aaggtggcta tctttattgc ccaccaggct ccttaatgac gtttgttaac
    11701 gcccaggccg aagacagcca aatcttttta tataagcgcc gcta
//
LOCUS       DAJTRM010000109        11675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRM010000109 DAJTRM010000000
VERSION     DAJTRM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.109"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            19..1659
                     /gene="trg"
                     /locus_tag="OO322_003370"
     CDS             19..1659
                     /gene="trg"
                     /locus_tag="OO322_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5908126.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1697..2620)
                     /locus_tag="OO322_003371"
     CDS             complement(1697..2620)
                     /locus_tag="OO322_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5908127.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            2837..4180
                     /locus_tag="OO322_003372"
     CDS             2837..4180
                     /locus_tag="OO322_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5908128.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4405..6060
                     /gene="opgD"
                     /locus_tag="OO322_003373"
                     /gene_synonym="mdoD"
     CDS             4405..6060
                     /gene="opgD"
                     /locus_tag="OO322_003373"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5908129.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6200..6424
                     /locus_tag="OO322_003374"
     CDS             6200..6424
                     /locus_tag="OO322_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5908130.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6487..7023
                     /gene="rimL"
                     /locus_tag="OO322_003375"
     CDS             6487..7023
                     /gene="rimL"
                     /locus_tag="OO322_003375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5908131.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7018..7998)
                     /locus_tag="OO322_003376"
     CDS             complement(7018..7998)
                     /locus_tag="OO322_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908132.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8122..9114
                     /gene="tehA"
                     /locus_tag="OO322_003377"
     CDS             8122..9114
                     /gene="tehA"
                     /locus_tag="OO322_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5908133.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9111..9704
                     /gene="tehB"
                     /locus_tag="OO322_003378"
     CDS             9111..9704
                     /gene="tehB"
                     /locus_tag="OO322_003378"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5908134.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10007..10663
                     /locus_tag="OO322_003379"
     CDS             10007..10663
                     /locus_tag="OO322_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5908135.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10681..11187)
                     /gene="tnpA"
                     /locus_tag="OO322_003380"
                     /pseudo
     CDS             complement(10681..11187)
                     /gene="tnpA"
                     /locus_tag="OO322_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11195..11675
                     /locus_tag="OO322_003381"
                     /pseudo
     CDS             11195..11675
                     /locus_tag="OO322_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3023 a         2735 c         2946 g         2971 t
ORIGIN      
        1 ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt tttgcatcac
       61 atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct attcgcatta
      121 agttctgctc tggctggcta tttcctctgg caggccgatc gcgatcagcg tgatgttact
      181 gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg
      241 atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcga
      301 aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg tgcttatcag
      361 aatcgaccgg tgaaaacacc tgctgatgaa gccttcgaca ctgaattaaa tcaacgcttt
      421 caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa
      481 gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt
      541 ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga actggcccat
      601 cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcttgc cctggtcatg
      661 acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca
      721 caacggattg aaaaaatcgc cagtggcgat ctgacgatga atgatgaacc ggcgggtcgt
      781 aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact ggggatgaca
      841 gtagggactg ttcgacaggg cgcggaagag atttatcgtg gcaccagcga aatttcagct
      901 ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga acaaactgcc
      961 gccagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc
     1021 agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggtttccggt
     1081 gtagtaaaaa cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc
     1141 gtcatcaaca gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc
     1201 gcgcgagcgg gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt acggacactc
     1261 gcaagccgca gcgctcaggc agcgaaagag attgaaggct tgatcagtga atcagtcagg
     1321 ttaattgacc tggggtcgga tgaggtggca acggcaggga aaaccatgag cactattgtt
     1381 gatgccgtcg cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa
     1441 agtagaggca taactcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag
     1501 aatgcttctc tggtagaaga ggcctcagca gcggcggtgt cccttgaaga acaggctgca
     1561 cgattaactg aggcggtgaa cgtattccgt ctgaacaaac attctgtgtc ggcagaacct
     1621 cgcggagcgg gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga
     1681 cagatccctt cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc
     1741 aaccagaaga gaacgtcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat
     1801 gtcccggaac aggcagggcc accagcgtgg catgacgcag gtcgtctttt acagcgccag
     1861 aagggacaaa ccacacgtaa tcgtattcaa cggtaagttg acgagatagc gaagtagaca
     1921 gcgtttcgat acaacccgaa ggaattttac agccctggct ctgcactaat gcatctgaat
     1981 gctggcgtgg cgcagtgcct tctggtgata caacgaccgg ccattccagc acccggctta
     2041 gcgttacgtt ctcctgaagt agcgggtgat tagggcggac aaccagcttc aacgattcaa
     2101 gaaacagcag ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc
     2161 cgatatcgat ttccccggtt tttaaacccg ccagaatcat agggttactc attgtcgcaa
     2221 cttgcaaggt cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc
     2281 ccagtgccgc agtaggtagt gcaccaaccc tgacgacatc attattaaga ccttctttac
     2341 gatgaagcga ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa
     2401 attgttcgcc gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc
     2461 ccgtcagctg ctccagttca ttcaatgtat tagagagcgc aggttgactc aaattaaggg
     2521 tttcagctgc gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc
     2581 gcaaacggat gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag
     2641 cggtgtcgct gacaagtgaa gttgtttgat tatgataact tgattgcaaa atattattaa
     2701 caattaaagc aattatgtta cagcaaaatg gataatattg atgttttcgc ggcgagatca
     2761 cagtttgtaa attcttcccg ccagagtgaa tgcggttacc tacactccag attactgacc
     2821 actggaggca gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc
     2881 gcaggcaatg tcagccatgt accagcaaga agttccgcaa tatggcacgc tgctggaact
     2941 ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct caactgcacg aaaaaatggt
     3001 aaatgcagac gagctggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac
     3061 tgcacaagag cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag
     3121 ctattacgat ctctcgcagg caggggtgcc ggtacattcg acagcatttc ggcccattga
     3181 tgatgcttct ctggcgcgta atcccttccg cgtttttaca tccttactcc gccttgagct
     3241 tatcgagaac gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt
     3301 caccccacgt tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac
     3361 acaggcacag gagtttgtgc aggaagccct ggaaacgttt cgctggcacc agttagcaac
     3421 ggtagatgaa gaaacctatc gcgccttgca caacgaacat cggttgattg ctgatgtggt
     3481 ctgttttcct ggatgccata tcaaccacct gacgccacgt acgctggata ttgaccgggt
     3541 gcagtcgatg atgcctgaat gcggaattga acccaaaatt ctcatcgagg ggccgccgcg
     3601 ccgcgaggta ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt
     3661 gtttgcgggg cagaaacagg gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg
     3721 cgtggcatta acgccgaaag ggcgacaact gtatgatgat cttctgcgta acgctggaac
     3781 cgggcaggat aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga
     3841 cagtgagttt ttaatgcgtc agcaagggtt ggcatggttc cggtaccgtc tgacgccttc
     3901 gggtgaggcg catcgtcagg cgattcatcc tggagacgat ccacagccct taattgaacg
     3961 tggttgggta gtggcgcaac ccatcaccta tgaagatttc ttgcccgtta gcgcggcggg
     4021 gatcttccag tcaaatctgg gtaatgaaac gcagacacgc agtcacggta atgccagtcg
     4081 cgaagcattt gagcaggcgt tgggttgtcc ggttttggat gagttccagc tttaccagga
     4141 agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg
     4201 caaaaatttg cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc
     4261 cgatttatgc atattctctc agttcaacaa ttggattatt aataaatatt gtctagagtg
     4321 agcggtcata aataagcact ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa
     4381 caccagaagg actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct
     4441 atggccgccg tgtgcggtac cagcggcatt gcttctcttt tttctcaggc agcattcgcg
     4501 gcagattctg atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca
     4561 atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg
     4621 ctggcgacaa tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc
     4681 tggcataacg ttgagaaccg tcaactggac gctcagttct tccatatggg aatgggattc
     4741 cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac
     4801 tttcgcccgg agttgttcaa atacaacgat gcaggtgttg ataccaaaca attagaaggg
     4861 caaagcgatc tcggctttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt
     4921 gatgtagtat catttctcgg cgcgagttat ttccgcgccg ttgatgatac atatcaatac
     4981 ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg acagtaaaga agagttcccc
     5041 gactttaccg ccttctggtt tgatacggta aaaccggggg caactacctt taccgtttat
     5101 gcgttgctcg atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa
     5161 agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg
     5221 ggcatttcgc cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat
     5281 acaattcatc cgcaaattca tgactctgat cgtctgtcca tgtggcgggg caacggcgag
     5341 tggatttgcc gtccgctgaa taatccgcaa aaattgcagt tcaatgctta caccgacaac
     5401 aacccgaaag ggtttggttt attgcaactg gatcgtgact tctcccatta tcaggacatt
     5461 atgggctggt ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag
     5521 ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgc
     5581 ttctggcagc cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg
     5641 tactggagtg cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc
     5701 ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg
     5761 cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc aaaaggcatt
     5821 gagccggtga ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa
     5881 cccatcgatg gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg
     5941 gtcgatatgc ggatgtatct acgttgtcag ggggacgcta tcagtgaaac atggctgtat
     6001 cagtatttcc cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt gatgagttaa
     6061 tcgttttttc ttcggcacct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg
     6121 catttttccc taagttaaat gagtaatctg atggtgtgta tttcagatac accgtgtcag
     6181 ccactaacag ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa
     6241 cgaaaatcct cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc
     6301 cagaaaggaa ggttccgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca
     6361 aaagctacag ttaaaagatg agatgctcaa aatcctgcag caggagagcg tcaaagaggt
     6421 gtaaactttc ctaagccgcc agccaggcgg cttttttaac aacagcatgg attgactgga
     6481 gataagatga ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa
     6541 agtcacgtca cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg
     6601 ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat
     6661 gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt
     6721 atcggcgtta tctcgtttaa tcgtattgaa ccactgaata aaactgctga aatcggctac
     6781 tggctggacg aatctcatca ggggcagggg atcatttctc aggcgctgca gacattgatt
     6841 catcattacg ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat
     6901 ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag
     6961 gctgagttcc tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca
     7021 taataaccct gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc
     7081 acggacatga accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac
     7141 atggttttca attatcaccg cacctgtaat acggctctca ccttgtatta caacgtgctc
     7201 gtcgagcaga atcggtccac cacgtaccac ggcatttccg ccaatcaaga catgatgttt
     7261 taacacacaa ttaccttcca caatggcata ttccgccacc tgcgaactgt aatgaatcgt
     7321 cggaatggca tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca
     7381 atcgcatagc cagacgttgt tctcttcatt accttcaaca ctggcaaaat caaaaacttc
     7441 ggcgcgatgt tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg
     7501 gacaatacgc gaggcgctaa cccttgcgcg atcataaatt tggagcagga gttgatggtc
     7561 gggcgttaag ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa
     7621 tattctacat tgcacgtaaa ctaatgagtc atgaatggtg acactgtcac ttatatatgc
     7681 accctggctg atttcactat tatcgatcca gacgttatcc gttgcgtaaa cctctcccca
     7741 cagcacgctg gtgccggtga tacgcgtatt accggaaatc accgttccgc cgaacgcaat
     7801 agcattctgg tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat
     7861 ccagccgcca gcggttcctg ctatcacatc gttaaaatcg ctaatggcga taatctgccg
     7921 taataacaca ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact
     7981 taggcgatat ttgcgcatag cgttttccca caggtgactt actataaccg tagcaaattc
     8041 tgcggctctg gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa
     8101 ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac
     8161 tttggtattg tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt
     8221 tggcaggtca gccactggtt aggggatggg ctggtgattc tggcgatgat catctgggga
     8281 ttattgacta gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcggaa
     8341 gttcgccatc cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg
     8401 gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt gcatgttcag ttttggtgtc
     8461 gtggttcagt tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct
     8521 gaagaagcta ccacgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca
     8581 atggcctgtg gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt
     8641 ttctcatggc taagccttga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta
     8701 cccacggcac tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt
     8761 gcctggctga gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct tttcggttat
     8821 ggactgctgc aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt
     8881 aatgcttcat tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat
     8941 ctggggagtg gcagcgataa tggatttttc catacgctgg cggtgccgct gtttatcttt
     9001 accaatttta ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa
     9061 ttgttagtca gaaccgagcg cgccgtttta atgaaagcag aggacaaaga atgatcattc
     9121 gtgacgaaaa ctattttact gataaatatg aattaacccg cacacactct gaagtactgg
     9181 aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta
     9241 acagtcttta cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga
     9301 gtatcgccaa cgtcgagcgc attaaatcca ttgaaaatct ggataattta cacacccggg
     9361 tcgttgatct gaataacctc acatttgatg gacagtacga ttttattctt tcgactgtgg
     9421 tgctgatgtt ccttgaggct aaaaccatcc cagggttgat tgccaatatg caacgttgca
     9481 ctaaacctgg tggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta
     9541 ccgtcggctt cacgtttgcc ttcaaagagg gagaattacg tcgatattac gaaggctggg
     9601 agagggtgaa atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc
     9661 gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa atgacccggt aagcacaaaa
     9721 cgcgtgaaaa ttccccacgc tgagatgatt tactgttctt cttttcggta agcatatttt
     9781 ttatcgaagg gatgtgaaat taatcacagt agtcgaagtt ttttagcagc ttaacttact
     9841 gaaatttaac tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa
     9901 aaggggctga aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt
     9961 tttgctataa cttagaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc
    10021 atttgcgaaa gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat
    10081 cacccagcca gataaatata acaattactc tgatttaaaa gaaacaacct cagcaacagg
    10141 taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt
    10201 ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag ggcagaaagt
    10261 tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa
    10321 accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt
    10381 agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc
    10441 ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga
    10501 gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa
    10561 agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga
    10621 catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg
    10681 ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa
    10741 ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga
    10801 tattggcgca cctccgcagc tactggcaaa atagccagga ctccacaaaa cgcctttgta
    10861 gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg atacgccttt
    10921 gaggctgttt accagactgg atatcgccag tttgggaggt tagttaatta acaaatggac
    10981 gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaataagcac atacatttga
    11041 aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa atctggcgtc ggtatctggt
    11101 gacaaagact aggtgaacat gcatcaggaa aacacaatgc cttccacgcc ggatatcggt
    11161 ttcttttttc acagaccaaa gtatcatttg acctgtgaaa cgattacagg catttaaatt
    11221 ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg cgcttcgcag gcgcatgtcg
    11281 tttcgttttc aatcgtgctc tggcacgtca gaatgagaat cataaggccg gtaataaata
    11341 catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca ctgaaacgca
    11401 atggcttaaa gattctccct cacagccatt gcaacagtca ctgaaagacc ttgagcgggc
    11461 ttacaaaaac ttcttccgga agcgggctgc ttttccccga ttcaaaaagc ggtgacagaa
    11521 tgatgcattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc gtatttttct
    11581 gccgaaactg ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa
    11641 tgtcactgtc agccagtcct gcggtacgtg gtaca
//
LOCUS       DAJTRM010000110        11629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRM010000110 DAJTRM010000000
VERSION     DAJTRM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.110"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..342
                     /locus_tag="OO322_003382"
     CDS             <1..342
                     /locus_tag="OO322_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5908136.1"
                     /translation="NIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQ
                     QLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQND
                     LLLRATGGVCV"
     gene            complement(459..734)
                     /gene="yjfN"
                     /locus_tag="OO322_003383"
     CDS             complement(459..734)
                     /gene="yjfN"
                     /locus_tag="OO322_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5908137.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(883..1212)
                     /gene="bsmA"
                     /locus_tag="OO322_003384"
     CDS             complement(883..1212)
                     /gene="bsmA"
                     /locus_tag="OO322_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5908138.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1394..2143
                     /gene="yjfP"
                     /locus_tag="OO322_003385"
     CDS             1394..2143
                     /gene="yjfP"
                     /locus_tag="OO322_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5908139.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2140..2895)
                     /gene="ulaR"
                     /locus_tag="OO322_003386"
     CDS             complement(2140..2895)
                     /gene="ulaR"
                     /locus_tag="OO322_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5908140.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3003..4067)
                     /gene="ulaG"
                     /locus_tag="OO322_003387"
     CDS             complement(3003..4067)
                     /gene="ulaG"
                     /locus_tag="OO322_003387"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5908141.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4422..5819
                     /gene="ulaA"
                     /locus_tag="OO322_003388"
     CDS             4422..5819
                     /gene="ulaA"
                     /locus_tag="OO322_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5908142.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5835..6140
                     /gene="ulaB"
                     /locus_tag="OO322_003389"
     CDS             5835..6140
                     /gene="ulaB"
                     /locus_tag="OO322_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5908143.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6150..6614
                     /gene="ulaC"
                     /locus_tag="OO322_003390"
     CDS             6150..6614
                     /gene="ulaC"
                     /locus_tag="OO322_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5908144.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6628..7278
                     /gene="ulaD"
                     /locus_tag="OO322_003391"
     CDS             6628..7278
                     /gene="ulaD"
                     /locus_tag="OO322_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5908145.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7288..8142
                     /gene="ulaE"
                     /locus_tag="OO322_003392"
     CDS             7288..8142
                     /gene="ulaE"
                     /locus_tag="OO322_003392"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5908146.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8142..8828
                     /gene="ulaF"
                     /locus_tag="OO322_003393"
     CDS             8142..8828
                     /gene="ulaF"
                     /locus_tag="OO322_003393"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5908147.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8957..9232)
                     /locus_tag="OO322_003394"
     CDS             complement(8957..9232)
                     /locus_tag="OO322_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5908148.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9559..9954
                     /gene="rpsF"
                     /locus_tag="OO322_003395"
     CDS             9559..9954
                     /gene="rpsF"
                     /locus_tag="OO322_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5908149.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9961..10275
                     /gene="priB"
                     /locus_tag="OO322_003396"
     CDS             9961..10275
                     /gene="priB"
                     /locus_tag="OO322_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5908150.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10280..10507
                     /gene="rpsR"
                     /locus_tag="OO322_003397"
     CDS             10280..10507
                     /gene="rpsR"
                     /locus_tag="OO322_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5908151.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10549..10998
                     /gene="rplI"
                     /locus_tag="OO322_003398"
     CDS             10549..10998
                     /gene="rplI"
                     /locus_tag="OO322_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5908152.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11069..>11629)
                     /locus_tag="OO322_003399"
     CDS             complement(11069..>11629)
                     /locus_tag="OO322_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5908153.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         2752 a         2857 c         3074 g         2946 t
ORIGIN      
        1 aatattatgt gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta
       61 ttgtcggaag catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt
      121 cgtttacagc agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag
      181 ctattcctgc tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag
      241 gcgtggtgtc aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag
      301 aatgatttat tgctgcgggc gacgggggga gtgtgtgtgt aagcgtatac gactgatgcg
      361 acgctggttt cgattaacta aatgaaatat gtgaaaattg taggccggac aaggcgctcg
      421 cgccgcatcc ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt
      481 acgcgccagt tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca
      541 ccttgttcat cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata
      601 ttgctaactg agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat
      661 tctgcagact gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca
      721 agtaattgtt tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat
      781 cgcggtaata aacgcccaag cttccagccg gtaatttatt gtgcacaggt aaacgcatag
      841 ggacgcaaag caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat
      901 ggcttgtgaa taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata
      961 atcagctttt gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc
     1021 ccgaaccatc gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat
     1081 ctcttgcgga tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca
     1141 ggcacttaac atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt
     1201 cctgctaacc atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt
     1261 tacctggtac gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt
     1321 atgaactaat accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa
     1381 aaaggagaga cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca
     1441 tgcttatcct gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt
     1501 tacttcatcc agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg
     1561 ggtgatcatg ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg
     1621 gttaaatcaa ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc
     1681 ggcaatagcc gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat
     1741 gggcgcgatg acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag
     1801 catgatggga tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc
     1861 tgaaacggca gcacagcaga atgaattcaa taaaattgtc gcgccgctgg cagagtggga
     1921 agcgacaaac cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga
     1981 cgatgacgtt gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg
     2041 acgggataaa ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga
     2101 ggcgttagat gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac
     2161 cccttgcgct tccagttgtt gcaggatttc cgggttagca tttttgccgg tgatcagcat
     2221 atcgatttga tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc
     2281 aaccagtacc accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag
     2341 catatcggtt ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg
     2401 cccggcatag agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt
     2461 gtactgtcca cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag
     2521 cggtagataa ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa
     2581 cgcggtggag ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc
     2641 tttagcaata cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca
     2701 gcgcgggcgc tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgct
     2761 ttcgccaagt ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac
     2821 gactttctca acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct
     2881 ttgtgcttca gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat
     2941 agccaggaaa tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca
     3001 gattacagga atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg
     3061 cgcggatagt ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc
     3121 tgccagataa acggcttgaa gccatacttc agacgatctt ttttgatctc ccacagcacg
     3181 cggatctctt gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc
     3241 actttcgcat tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta
     3301 ataccgcgcg ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg
     3361 tgcttcgcat aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg
     3421 gttttgaaca ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct
     3481 ttttgatcgg caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt
     3541 tcaatgtctt tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt
     3601 acgccccagc caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca
     3661 gcacagttct gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga
     3721 gtcgccagta ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg
     3781 cgtaggtttg gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc
     3841 attaacgggt taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg
     3901 gtgccacctt ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg
     3961 ccaggggcga cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa
     4021 gtgctcagga tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt
     4081 catgatagtt caattcgaat caacatgtga ttggttttga ttaatcctga cactattcct
     4141 tcaggaaggt aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt
     4201 ggggaaagat ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg
     4261 tttcgttgtg tgaatcccac tctatccatg tggaattatt tgcgggtcgc gtcacattta
     4321 atcataaata atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg
     4381 gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca
     4441 tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga
     4501 cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta
     4561 aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca
     4621 aaccggtggt ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt tccgatactt
     4681 acgcgtcgat gatggcaacc atcgaccgca tgggcgatgc ctatagctgg gtaggttacg
     4741 cggtgttgtt agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc attaccggta
     4801 ttcgtaccat catgctgact ggtcatatca tgttccagca ggcggggctg attgccgtta
     4861 cgttgtttat tttcggctac tccatgtgga caaccattat ctgcacggcg attctggttt
     4921 cgctctactg gggcattacc tccaacatga tgtacaagcc gactcaggaa gtgacggacg
     4981 gctgcggttt ctccatcggt caccagcaac agtttgcatc attaattgcc tataaagttg
     5041 cgccgttcct cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca ggctggctga
     5101 atattttcca cgacaacatc gtctccacgg cgattgtcat gaccatcttc tttggtgcca
     5161 ttctgctctc cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg cactggacgg
     5221 tctatatcct gcaaaccggt ttttccttcg cggtggcgat cttcatcatc actcagggtg
     5281 tgcgcatgtt tgtggcggaa ctctctgaag cgtttaacgg tatctcccag cgcctgatcc
     5341 ctggtgcggt tctggcgatt gactgtgcgg ctatttatag cttcgctccg aacgccgtgg
     5401 tctggggctt tatgtggggc accatcggtc agctgattgc ggttggcatc ctggtcgcct
     5461 gcggttcctc gatcctgatt atcccaggct ttatcccgat gttcttctcc aacgccacca
     5521 tcggcgtgtt cgctaaccac ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga
     5581 tggggatgat cgaaatcttt ggttgcgtct gggtggtgaa acttaccggt atgagtgcct
     5641 ggatggggat ggcggactgg tcgattctgg caccgccgat gatgcagggc ttcttctcca
     5701 tcggtatcgc ctttatggcc gtcatcattg taattgcact ggcttatatg ttcttcgctg
     5761 gccgcgcact gcgcgcagaa gaagatgcag aaaaacaatt ggcagaacag tctgcttaat
     5821 aaggagtttt gattatgacc gtacgtattc tggctgtgtg tggcaacgga caaggcagtt
     5881 ccatgatcat gaagatgaaa gtggaccagt ttttaaccca atcaaacatt gaccacacgg
     5941 taaatagctg cgcggttggc gaatacaaaa gcgagttgag cggcgcggat atcatcatcg
     6001 cttctacgca cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc
     6061 gcaacatgct ctctcctgcc gactttggcc cgaaactgct ggaagtgatc aaagcgcatt
     6121 tcccgcagga tgtgaagtaa ggacgcgcca tgaaattaca tgattcgctg gcggagaata
     6181 aatccatccg cctgcaggct gaagctgaga catggcagga cgcagtaaaa atcggtgttg
     6241 acctgctggt ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg
     6301 ttgaacagca cggtccgtac tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc
     6361 cggaagaggg cgttaagaaa accggttttg cgctggtgac gctgaaaaag ccgctggagt
     6421 tcaaccacga agataacgac ccggtggata tcctcatcac catggcggcg gtcgatgcca
     6481 acacgcacca ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat gaagaaaatt
     6541 tcgaccgttt acgtgcctgc cgtaccgagc aggaagtact ggatctcatt gaccgcacca
     6601 acgcggcagc ttaagaagga attgaagatg tcattaccga tgttgcaagt cgcgctggac
     6661 aaccagacta tggatagcgc ctacgaaacc actcgcctga ttgccgaaga agtcgacatt
     6721 atcgaagtgg gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa
     6781 gcgctctacc cgcacaaaat cgtgctggca gacgccaaaa ttgccgatgc gggcaaaatt
     6841 ctttctcgta tgtgcttcga agccaacgct gactgggtga ccgtgatttg ttgtgcggat
     6901 atcaacaccg ccaaaggtgc gctggacgta gcaaaagagt ttaacggcga cgtgcagatc
     6961 gaactgaccg gttactggac ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag
     7021 caggtggttt atcaccgcag ccgtgacgcg caggccgctg gcgtggcgtg gggtgaagcc
     7081 gatatcactg cgatcaaacg tctttccgat atgggcttca aagtcaccgt caccggtggc
     7141 ctggcgctgg aagatctgcc gctgttcaaa ggcattccaa ttcatgtgtt tatcgccggt
     7201 cgcagcatcc gtgatgccgc ttctccggtt gaagccgcgc gtcagttcaa acgttccatc
     7261 gccgaactgt ggggctaagg agcggatatg ttgtccaaac aaatcccgct tggcatctat
     7321 gaaaaagcgc tccccgccgg ggagtgctgg ctggaacgcc tgcgactggc aaaaacgttg
     7381 ggcttcgatt ttgtcgagat gtcggtagat gaaactaacg agcgcctgtc gcgccttgac
     7441 tggagccgcg aacagcgtct ggcgctggtg aatgctattg ttgaaaccgg cgtgcgtgtg
     7501 ccgtccatgt gcctttctgc ccatcgtcgt ttcccgttgg gcagcgaaga tgacgcggtg
     7561 cgggcgcagg ggctggagat tatgcgtaaa gccatccagt tcgcccagga cgttggcatt
     7621 cgcgtgatcc agctggcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt
     7681 cgtcgtttcc gtgacggcct gaaagagagc gttgagatgg cgagccgcgc gcaggtgacg
     7741 ctggcgatgg agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac
     7801 gcgcactatc tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg
     7861 tgggacaacg atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat
     7921 gtgaaagaca ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat
     7981 ttcgaacgtt gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag
     8041 atgtggagcg aaacggcgga agacccggcg gcagaagtgg tgaaagcgtg tgattgggtg
     8101 aaagcgcgca tggccaaagc aggcatggtg gaggcggcat aatgcaaaag ctaaaacagc
     8161 aggtgtttga agccaacatg gatctgccgc gctatgggct ggtgaccttt acttggggca
     8221 acgtcagcgc tatcgaccgc gaacgcgggc tggtggtgat caagcccagc ggcgttgcct
     8281 acgaaaccat gaaagcagac gatatggtgg tggtcgatat gagcggcaag gtggtggaag
     8341 gggagtatcg cccatcttcc gacactgcga cgcatctcga actctaccgt cgttacccgt
     8401 cgcttggcgg cattgtccat acccactcca ctcatgccac cgcatgggcg caggcggggc
     8461 tggcgatccc ggcattaggc accacgcacg ctgactactt ctttggtgat atcccgtgta
     8521 cgcgtggatt aagcgaagaa gaggtgcagg gtgagtatga actgaacact ggcaaagtga
     8581 ttatcgaaac gctgggcaac gccgagccgc tgcatacgcc gggaattgtg gtgtatcagc
     8641 acggaccgtt cgcctggggg aaagatgccc acgacgcggt gcataacgcg gtagtgatgg
     8701 aagaagtggc gaaaatggcg tggattgcgc gcagtattaa tccacaactc aatcatatcg
     8761 acagctacct gatgaataaa cacttcatgc gtaagcacgg tcctaacgct tattacgggc
     8821 agaagtaaaa cacgcgctgc ggaaatttcc ttcctcggga gataactggt ctaattccgc
     8881 agccgttttt caaaaaaagc cccctgcgaa gggggcaaag caaactatgg caatgtttcg
     8941 ttggttatac ctggtgctag cgataaatat ccgcgctggc gtgcatattg ccgttactcc
     9001 ccggttcccg catcagaatt acgtggtagt acgttgcgcc ttgcgcatct gtttcttcat
     9061 ttaatgcctg acgtgcttcg ctttcagtgg cgaaattatg attgatataa atcacgccta
     9121 agctttgcac atcgtccata tttctggcct ggtggttatt aatttcaatg gctgcccatg
     9181 tatttgcact tagcaaaagc acagccagaa gggctaaaac tcgactgaac atagatacct
     9241 cctcgacggc tgactttgtg tgctctcctc tgtgatgatc ttctgattta attttaatca
     9301 atgataaaga agttgatggt gaccatttct gatgcagttg ttcaaaaaaa cactgtgacg
     9361 gaatgtgatg aacttcaaat cagcggttta gaggttattt gcgaaagggg agatttattt
     9421 ctgctccgcc cttgagttta gcggggtatg ccagtactat aacgcgtcat ttttcagccg
     9481 acctttaaca cgttccttgc ctccccggga ttcggctgac ccagacagga ggctgaataa
     9541 tccgtaagga gcaattcgat gcgtcattac gaaatcgttt ttatggtcca tcctgaccag
     9601 agcgaacagg ttccgggcat gatcgagcgc tacactgctg ccatcactgg tgcagaaggc
     9661 aagatccacc gtctggaaga ctggggccgc cgtcagctgg cttacccgat caacaaactg
     9721 cacaaagcac actacgttct gatgaacgtt gaagctccgc aggaagtgat cgatgagctg
     9781 gaaactacct tccgcttcaa cgatgccgtt atccgcagca tggttatgcg taccaagcac
     9841 gctgttaccg aagcatctcc gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat
     9901 gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg attctgaaga gtaatttctg
     9961 atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt
    10021 ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc
    10081 ggctttcacc ggcaggcatg gtgtcaaatg cccgttattg ttagcggaca cgaaaaccag
    10141 gccattactc acagtataac ggtcggcagt cgcataaccg ttcaggggtt catttcatgc
    10201 cacaaggcaa agaacggact gagcaaaatg gttttgcatg ccgagcagat tgaattgata
    10261 gattctggag actagccata tggcacgtta tttccgtcgt cgcaagttct gccgtttcac
    10321 cgcggaaggc gttcaagaga tcgactataa agatatcgct acgctgaaaa actacatcac
    10381 cgaaagcggt aagattgtcc caagccgtat caccggtacc cgtgcaaaat accagcgtca
    10441 gctggctcgc gctatcaaac gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca
    10501 tcagtaatcg gtcacggtcc attaatacga ctttgagagg ataaggtaat gcaagttatt
    10561 ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc
    10621 tatgctcgta acttcctggt accgcagggt aaagctgttc cagctaccaa gaaaaacatt
    10681 gaattcttcg aagcacgtcg cgctgaactg gaagctaaac tggctgaagt tctggcagct
    10741 gctaatgctc gcgctgagaa aatcaatgca ctggaaactg ttaccatcgc gtctaaagct
    10801 ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg acatcgctga cgctgtaact
    10861 gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc
    10921 actggcgaac acgaagtgag cttccaggtt cacagcgaag tattcgcgaa agtgatcgta
    10981 aacgtagtag ctgaataatt cgttattcaa cgagacgtaa aaacgccgac cattggtcgg
    11041 cgttttgctt tctatttttc gtcaggcatt aatttcgcaa gtagatccat tcaccattct
    11101 cttgtgtcca taagtcagga tggtttgctg gcgttagatg catacgatca ctccttatgt
    11161 ctcggtcctg cggcactcgc tcgaaaccta aacccgagta gattgcggct gaatttgata
    11221 aggaacgtag accaattttc aaatcatggg gtaaatcaga atacgcgttt agcaatgcag
    11281 caatgatgaa tccgagggca ttagaaacgc ctgtaacagt gtgaagttgg tcgatataaa
    11341 gatcgattgt tggtagttca gatgagcagc aattaggcat ttgggcagga agggttacaa
    11401 cgtatcctat cgtttggccg gtatagtctg gagtactata accaggtccc aaaacaacct
    11461 cgtgtattgt gtatttactg ttgggttcga aggattcgac atcttgcaat aaatatttca
    11521 gttctgcggt taacctcgca cccgcgtaag ctttattgta tttctgggcg tgaatattat
    11581 ctaatgtagc ttcatggttc catccaaaag aattatcacc attcattgt
//
LOCUS       DAJTRM010000111        11578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRM010000111 DAJTRM010000000
VERSION     DAJTRM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.111"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(282..357)
                     /locus_tag="OO322_003400"
     tRNA            complement(282..357)
                     /locus_tag="OO322_003400"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO322_003401"
     CDS             916..3168
                     /locus_tag="OO322_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908154.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4436
                     /gene="csiD"
                     /locus_tag="OO322_003402"
     CDS             3504..4436
                     /gene="csiD"
                     /locus_tag="OO322_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5908155.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4456..5724
                     /gene="lhgO"
                     /locus_tag="OO322_003403"
     CDS             4456..5724
                     /gene="lhgO"
                     /locus_tag="OO322_003403"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5908156.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5747..7195
                     /gene="gabD"
                     /locus_tag="OO322_003404"
                     /pseudo
     CDS             5747..7195
                     /gene="gabD"
                     /locus_tag="OO322_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7203..8489
                     /gene="gabT"
                     /locus_tag="OO322_003405"
                     /pseudo
     CDS             7203..8489
                     /gene="gabT"
                     /locus_tag="OO322_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8727..10127
                     /gene="gabP"
                     /locus_tag="OO322_003406"
     CDS             8727..10127
                     /gene="gabP"
                     /locus_tag="OO322_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5908157.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10148..10810
                     /gene="csiR"
                     /locus_tag="OO322_003407"
     CDS             10148..10810
                     /gene="csiR"
                     /locus_tag="OO322_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5908158.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO322_003408"
     CDS             complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO322_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5908159.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATANQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11344..11502)
                     /locus_tag="OO322_003409"
     CDS             complement(11344..11502)
                     /locus_tag="OO322_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5908160.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2979 a         2841 c         2887 g         2871 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 gggggaagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg
     4081 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
     4141 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
     4201 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
     4261 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     4321 tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     4381 tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc
     4441 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     4501 accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag
     4561 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     4621 tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc
     4681 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct
     4741 cagctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     4801 cgcgagtgga taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt
     4861 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca
     4921 aaaatcttcc aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt
     4981 gagcataaaa acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg
     5041 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     5101 ggctttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     5161 attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat
     5221 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     5281 cgcgaaggct atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg
     5341 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     5401 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     5461 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag
     5521 ctgattgacg attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc
     5581 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     5641 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     5701 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     5761 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     5821 tgaagtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     5881 gggcgcagat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg
     5941 tgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca
     6001 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc
     6061 gaaaggcgaa atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg
     6121 tatttatggc gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca
     6181 gccgattggc gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg
     6241 taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac
     6301 gccgttctct gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt
     6361 gtttaacgtg gtcaccggtt cggcaggcgt ggtcggtaat gaactgacca gcaacccgct
     6421 ggtacgcaaa ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg
     6481 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     6541 tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc
     6601 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     6661 tgccgaaaaa ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg
     6721 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     6781 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg
     6841 caacttcttc cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga
     6901 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     6961 acaagccaat gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg
     7021 cgtcttccgc gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat
     7081 ttccaatgaa gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     7141 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     7201 gaatgcgaag gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     7261 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     7321 tttaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     7381 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     7441 tccaggtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     7501 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     7561 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt
     7621 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     7681 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     7741 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     7801 aagatatcgc cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     7861 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     7921 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     7981 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg
     8041 gcgtcaccgg gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct
     8101 atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg
     8161 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga
     8221 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg
     8281 agctgtttga agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg
     8341 cacgcgcccg cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     8401 gcatccttgt accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg
     8461 gtcagtgttt tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc
     8521 gtcttgtccg gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc
     8581 tgtacgtgaa caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa
     8641 aagaatcaat aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa
     8701 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg
     8761 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     8821 tgtttgtcgg ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc
     8881 tgttcgccgg attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca
     8941 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct
     9001 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca
     9061 tcgccgccat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     9121 tcattaccct cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     9181 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     9241 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     9301 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     9361 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa
     9421 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     9481 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     9541 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     9601 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     9661 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     9721 aaaccccgta cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga
     9781 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     9841 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     9901 aaggaagcga aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg
     9961 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
    10021 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
    10081 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
    10141 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
    10201 cggtaatttt caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact
    10261 tggcgttggg ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt
    10321 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
    10381 cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga
    10441 tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
    10501 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
    10561 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
    10621 ggcgcgttat cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga
    10681 taaacacgat cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc
    10741 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
    10801 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
    10861 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
    10921 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggttggcag
    10981 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
    11041 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
    11101 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
    11161 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga
    11221 gtttttctcc ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg
    11281 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
    11341 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
    11401 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat
    11461 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
    11521 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagc
//
LOCUS       DAJTRM010000112        11393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRM010000112 DAJTRM010000000
VERSION     DAJTRM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.112"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..2342)
                     /locus_tag="OO322_003410"
     CDS             complement(<1..2342)
                     /locus_tag="OO322_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5908161.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO322_003411"
     CDS             complement(2276..2899)
                     /locus_tag="OO322_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5908162.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO322_003412"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO322_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO322_003413"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO322_003413"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5908163.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO322_003414"
     CDS             7507..8229
                     /locus_tag="OO322_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5908164.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11393)
                     /gene="yfaL"
                     /locus_tag="OO322_003415"
                     /pseudo
     CDS             complement(8357..11393)
                     /gene="yfaL"
                     /locus_tag="OO322_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2611 a         3190 c         2840 g         2752 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca ggccccctgc ttctgattac
     8881 cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg ttatcggcgc
     8941 gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca ccaaccgccc
     9001 caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg ctaagctgca
     9061 ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga taacgtccgc
     9121 cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt cgctccatca
     9181 taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg gcattcagca
     9241 ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc ggcgtgggat
     9301 caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct tgcgatcgca
     9361 gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg ttgacgtagc
     9421 cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca gctgcgaaat
     9481 caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag ttaaccacca
     9541 cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc gagtcatcgc
     9601 cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg tttaccgtca
     9661 gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg gtcagatcac
     9721 cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg gtattaagct
     9781 cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg ttaaatagtg
     9841 aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta ccatcgctga
     9901 tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc gcgccagtaa
     9961 cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata tccgcaacat
    10021 cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg ctggcggtca
    10081 gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga tgctgcccgc
    10141 tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact gccacttcac
    10201 cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga gtatcgacat
    10261 tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc tggcggttat
    10321 agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag gcgatttgtg
    10381 ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc gcatcgacca
    10441 ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca cctgcaagat
    10501 ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca aggccatcat
    10561 tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca ccgccgttta
    10621 acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg ccttccagcg
    10681 aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac tgcgcccctg
    10741 ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg atgatgccag
    10801 caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa gtgccattct
    10861 gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt agctcagcct
    10921 gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct
    10981 ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta acttcaccgt
    11041 tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc agatcgccgg
    11101 aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg tcattctctg
    11161 tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca cttaagccga
    11221 gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct gcgctattgt
    11281 tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa atatcaataa
    11341 gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg gct
//
LOCUS       DAJTRM010000113        11366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRM010000113 DAJTRM010000000
VERSION     DAJTRM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.113"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..360)
                     /locus_tag="OO322_003416"
     CDS             complement(<1..360)
                     /locus_tag="OO322_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5908165.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLAD"
     gene            complement(371..760)
                     /gene="cheY"
                     /locus_tag="OO322_003417"
     CDS             complement(371..760)
                     /gene="cheY"
                     /locus_tag="OO322_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5908166.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(775..1824)
                     /gene="cheB"
                     /locus_tag="OO322_003418"
     CDS             complement(775..1824)
                     /gene="cheB"
                     /locus_tag="OO322_003418"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5908167.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(1827..2687)
                     /gene="cheR"
                     /locus_tag="OO322_003419"
     CDS             complement(1827..2687)
                     /gene="cheR"
                     /locus_tag="OO322_003419"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5908168.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2706..4307)
                     /gene="tap"
                     /locus_tag="OO322_003420"
     CDS             complement(2706..4307)
                     /gene="tap"
                     /locus_tag="OO322_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5908169.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4353..6014)
                     /gene="tar"
                     /locus_tag="OO322_003421"
     CDS             complement(4353..6014)
                     /gene="tar"
                     /locus_tag="OO322_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5908170.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6159..6662)
                     /gene="cheW"
                     /locus_tag="OO322_003422"
     CDS             complement(6159..6662)
                     /gene="cheW"
                     /locus_tag="OO322_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5908171.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6683..8647)
                     /gene="cheA"
                     /locus_tag="OO322_003423"
     CDS             complement(6683..8647)
                     /gene="cheA"
                     /locus_tag="OO322_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5908172.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8652..9578)
                     /gene="motB"
                     /locus_tag="OO322_003424"
     CDS             complement(8652..9578)
                     /gene="motB"
                     /locus_tag="OO322_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5908173.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9575..10462)
                     /gene="motA"
                     /locus_tag="OO322_003425"
     CDS             complement(9575..10462)
                     /gene="motA"
                     /locus_tag="OO322_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5908174.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10589..11167)
                     /gene="flhC"
                     /locus_tag="OO322_003426"
     CDS             complement(10589..11167)
                     /gene="flhC"
                     /locus_tag="OO322_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5908175.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11170..>11366)
                     /locus_tag="OO322_003427"
     CDS             complement(11170..>11366)
                     /locus_tag="OO322_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5908176.1"
                     /translation="QMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIML
                     STRLLNDVNQPEEALRKKRA"
BASE COUNT         2573 a         3192 c         2791 g         2810 t
ORIGIN      
        1 atctgccaga aattgtcgtg tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg
       61 atcggcaaac cagtcatccc aacgttgggt taacgctttt gctgatttct ccatttgatc
      121 ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc tccgcagcct gggcggtcat
      181 ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg gcttccgccg cttcggcaat
      241 ggcctgaacc agccccagtt cccgcaaact gtcgcgcagc atatgcgtca ggctgccgat
      301 gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat
      361 agtcgcatcc tcacatgccc agtttctcaa agattttgtt gagtttttcc tccagcgtcg
      421 cggcggtaaa tggcttcacc acatagccac tggcccccgc ttgcgccgca gcaatgatgt
      481 tctctttctt cgcttctgca gtcaccatta acactggcaa tgccgacatc gcgccatccg
      541 cacgaattgt tttcagcaat tccaggccat ccatattggg catgttccag tcggagataa
      601 caaatccata accgcctgcc tgcaacttat tgagagcgtc gacaccatct tccgcttcct
      661 caacattatt gaatcccagc tctttcagca ggttacgcac tatgcgtcgc atggtggaaa
      721 agtcatccac aaccaaaaat ttaagttctt tatccgccat ttcacactcc tgatttaaat
      781 acgtatcgcc tgtccggcac taattgttgc caacatttgc tggcttacct ggctaagatc
      841 gaccacttcg cagacaccac ccatattgat ggcctcgcgc ggcatgccga acaccacgca
      901 acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca acattcccgc
      961 cgcgccgtcg ttgcccatac cggtcaggat caccccaacc gcattacgcc ccgcctgttt
     1021 ggcgacagaa tggaacaaca catctaccga aggccgatga cggttaaccg ccgggccgtc
     1081 gtgaattttg atttgataat ttgcgccact acgcgccagt tccatatgcc gatcgcccgg
     1141 cgcaatatag gcatgccccg gcaagacacg ttctccgtct tcggcttctt taaccccgat
     1201 ctggcaaagc ttattaagtc tgtcggcaaa agagcgggtg aaaccgggcg gcatatgttg
     1261 ggtaattaac aatgccgggc tggaaagcgg caacggttgc agtacgtgac gaattgcctc
     1321 agttccaccc gttgaagcac caatcgcaat cagtttttca gaactcaaca acggccctgc
     1381 cttcagcgtt gtcggtgctg acaatggctt atgtgctgca aggctcgcct ttgctgccgt
     1441 acgcaccttt tcagcaatca tttcgctata cgccagcatt ccttcgcgaa tacccagttg
     1501 cggtttggtg acaaaatcta ttgcccccag ctccagcgcg cgcagcgtga cttctgatcc
     1561 tttgccggtc aaggaagaaa ccatcacaac gggcattgga cgcaaacgca ttaatttttc
     1621 gaggaaatcc agtccgtcca tccgcggcat ttcaacatcc agcgtcagca catcgggatt
     1681 gaatttctta atcaagtcac gcgcaaccag cggatcaggc gcggtcgcca ccatttccat
     1741 gtcgctatgg ctgttgatga tttctgtcat gatctggcgc atcagtgccg aatcatcgac
     1801 agataacacc ctgattttgc tcatcgttaa tccttactta gcgcatacac cgtctgacca
     1861 cgcagcgtga agcggcgctc gaggtggcta aagttttcag agtgacccgc aaacagcaat
     1921 ccgtcgggtt taaggagcgg aacaaagcgg cgcaaaatct cctgctgggt agtttgatcg
     1981 aagtagatca tgacgttacg acagaagatc gcatcaaacg gccccggcac ggtgtactgt
     2041 ttcgccagta aattcagcgg ggcaaaatca acatagttcg ccagctcctg acgcacgcgt
     2101 accagccctt catgcggtcc cgtccctcgc atgaaatacc gttgcagttg ctgcggcgtc
     2161 aggtttttca actcttcatg gcgatagata ccgcttctgg ctttttccag cacttcggtg
     2221 tcgatatcac tggcaaacac tttccagcgt ccgggcacgg tgcccaatgt gtcagccaga
     2281 gtcatcgcaa tgctgtacgg ctcttcgccg gtcgaagccg ccgcgctcca tacgcgatcc
     2341 tcgccagaac ggcgacgtgc gtgatccgcg agcagaggga aatgatgagc ctcacggaaa
     2401 aatgccgtca gattcgtggt cagcgaattg ataaacgcct gccactcacc gctgtgctga
     2461 ttagattcca gcaagttcag ataatgaccg aaatccgtca gtcccagaga acgcaaacga
     2521 cgaaccagtc ggttgtaaac catgtcgcgt ttatggtcag ccagaacgat cccggctcgt
     2581 tgatagatca attgacttat ccgccgaaaa tgcgcgtcgg aaagcgccag gcgctcggtc
     2641 atctgtaaca ataaagacgt ttgcccacag ggcagagatg aagtcatagc gccttctcaa
     2701 tcacttcagg ataccactgg cgcaatttgt aactgcgccg actcatgtcg tgctacttca
     2761 tgttcttcaa gggtaaatac cgccacacgc gacgaaagat ggtcggcctg attcgccagt
     2821 tgttccgttg ccaccgccgc ttcttctacc agcgaggagt tctgctgagt cacctgatcc
     2881 atctggctga cagcctgtgc aacctgctca atcccccgcc gttgttcttc cgacgccgaa
     2941 gcaatttctc ccataatgtc gttcacgcgg gtcaccgaac tgacaatatc ggtcatggtc
     3001 gcggcggcgt tattcaccag tttcgaaccc tgctggacac gattgactga ctcttcgatc
     3061 agccctttga tctcttttgc tgcctgcgcg ctacggctgg caagattgcg cacttcacct
     3121 gccactaccg caaaaccacg cccctgctct ccggcgcgag ccgcttccac tgccgcattc
     3181 agggccagaa tattggtctg gaaagcaatt ccgtcgataa cgctgataat gtcgccaatt
     3241 ttttgcgagc tggtggcgat ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg
     3301 cccgcctgcg ccgttgtcgc ggcatttttt gccagttccg acgcctgtcg tgcgttatcg
     3361 gcgttttgcc ctaccgtggc ggttaattgc tccatactgg cggccgtttg tgccagcgat
     3421 gccgcctgct gttcggtacg acttgagaga tcgttattgc ctgcgacaat ctccgcgatc
     3481 ccaatgtgca tctcatggct tcccttacgc acatcactca ccgtcccacg caaagcctgc
     3541 tgcatggtct tcaggctggc aaaaatggcg gtgatctcat tacgaccata taccgcaatc
     3601 ggacgcgcca gattacccgc agcaatgctg tcaaaatggc taccgataat ggccagtggt
     3661 tgaacaatca tcttgcgcgt ccaccacagc gcactgctga tgtagatcgc cgcaacaata
     3721 atcatgctga taaacaccag cgccgaaatc tgatagttac gctggctttg cgcactggcg
     3781 gcttccagca catggttgat ctccagctgc caggcctcaa agttaacgtc aaacgcattc
     3841 tgtgatccct gcaccggcgc agtgaggaaa tccgaaagtt gattactttc aagccaggtc
     3901 gcctgatgtt cgagatcgtt gtgccagcgg gcaaaacttt tctccgtctc tttttgcaat
     3961 gccctgacgt gctcgttgcc cgcagtcatc gccataaaac ttttaaacag cgtggtggat
     4021 tgcgtcagac tggcgcgcgc cgtcgtcatc aacgttttaa tgtcatccgc cggatagcta
     4081 agcgctgtca gagtgcctgc tttgttcagc gcggtactgg cctgtaacat taccgcccga
     4141 gtttgcgcta atgccgcacg ttgctgattg ctctgctcgc cgtgattcag tcgttgcaaa
     4201 tcgtcgcgaa atgcccaaaa agacatgccg ttactgccaa tctgcaagat cccgcagaga
     4261 atcaaaatta aaaacagcgt ggtcgaaatt cgaatacgat taaacatcaa cgctccccat
     4321 caggcggcaa tgaccgcgtt agtaaaaact cgtcaaaatg tttcccagtt tggatcttgt
     4381 tcagtaattc gcagtcgtgg ctgtgccggt ggttgctcac tggcaggacg ggatggtgtt
     4441 tgcggtttat tggtgagtgg gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa
     4501 cgactcgcct gttcttccag cgcagctgcg gcggcagctg attcctgcac cagcgatgcg
     4561 ttctgttgcg tgacgcgatc catttccgaa accgccaatg cgacttgatc gatgccacgg
     4621 ctctgttcat ccgatgccga tgcaatctcg cccataatgt cagtcacgcg agtgacagca
     4681 ttgacgatat tgttcattgt ttccccggcg ctttcgacca gcaccgaacc ggtatcaatg
     4741 cgcgagacgg agtcttcaat gagggctttg atctcttttg ccgccagggc gctgcgactg
     4801 gcaagattac gcacttcacc cgccaccacg gcaaaaccac ggccctgttc acccgcacgc
     4861 gcggcttcaa ccgcggcatt cagcgcgagg atattagtct ggaaggcaat accgtcgata
     4921 acgctgataa tgtcggcaat tttcttcgaa ctatcggcga tctcatgcat cgttttcact
     4981 acgccatcca ccactttgcc gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc
     5041 gaggcctggc gggcgttatc ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg
     5101 gcggcagttt cttccagcgc ggatgcctgc tgttcagtac gggaggaaag atcggtgttg
     5161 cccgccgcaa tttcacgggt accggcatag atggcatctg aaccttcgcg gacatgagtg
     5221 acggtgtcag tcaaagagcg ttgcatatgt gaaacgctct gcgccaggtc acccatttca
     5281 ctgcgcccgt caatggtcag ggtattcgcc aggttaccac cggcgatttc gcaaatgtga
     5341 gcaataattt ttgccagcgg agtaagcaac atacggcgaa tgccgtacca cgccaccagc
     5401 agaatcaata ccaccaccag cgcgataacc gccagttgcc actgggcaaa tcggtaatca
     5461 tctgcgttgt cagtgacgat atcgcgatac agtttttcac tgctgagggc gtactgagca
     5521 aacgcttcgc ccattgcatt ttgcattccc tgggttggct gagcgaaata agctccagta
     5581 ttgccataat caagataatc aatcagttca gttaacgctg tgtaatagtt tttatatttt
     5641 tcatcaaaat tacggctggt agcgaccatt tcaggtaacg gtgccatgct tttgaatttt
     5701 ttataatgcg tcgcggcctg ggccaatgtt ttcctggcgc tatcgagcaa ttcaactttg
     5761 gcgttacttt gttgattgga ggaatccatc atcatccgca ccgctgaacg actcaggtta
     5821 atgcgcgttt gcagcattaa atcccaggtt gacgtcagct cgccctgctg ttcccgtaat
     5881 tgattggaaa ccacaaagct cttctggcta tggtgaaggg aagaaaaaaa caaactgccg
     5941 gaaataagct gtaacagtgc gaataccccc agcaccatta ccaacagcgt gactacgcgg
     6001 atacggttaa tcataaggca ccttcctgaa aacaagttga tctcgttatc ggcaagaagg
     6061 ggggaaactt tattgctgat gccacccgcc gcgaaattga aataaaaaac ccgatgcgca
     6121 gatcatcggg ttcatttcaa ttgaggaaat cgggagaatt acgccacttc tgacgccgcg
     6181 ctatctaaca gcgccatctc ttcgctgttc agcagttttt cgatgttcac cagaatcaac
     6241 atacggtcgc ccagtgcgcc cagtccagtg agatattctg ttgaaagcgt cacggcaaac
     6301 tctggtgccg gacgaatttg ctccgccgtc aatgaaagca cgtctgaaac gccgtcaacc
     6361 acgatgccga ccacccgctg tccgagattc aggacgataa ctaccgtgtt gtcgttatag
     6421 tccacatcca cctggctgaa cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc
     6481 agattcgtga cgcctttgat aaacgctggc gtgttcgcaa tccgtgttac ctgatcgtag
     6541 ccacggatct cctgcacttt caggatatca ataccgtact cttcatcacc aagggtaaat
     6601 accagaaatt cctggcctga cggctcgctg gccagctttg ttacattcgt cataccggtc
     6661 atattgttac ctttttactc attcaggcgg cggtgttcgc catacgttgt tcgcggttta
     6721 tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct gccgtcgcca agaatggtcg
     6781 cagcagaaat gccggggact ttgcgatagt tactttcaag gtttttaacc acaacctggt
     6841 gttgaccaat taattgatcc accagcaagg cgtagcggcg accgccactt tgtaagatca
     6901 ccacaattcc ctgggtggct tcggttttcg cgcccgcgac gttgaacact ttccacagtt
     6961 cgacgatggg cagatattca ccccgcactt ccagcacccg ctcgccgccg gccagtggat
     7021 ggagatcggc ttcacggggt tgcagtgatt ccataacagc attcagcggc agaatgaaaa
     7081 cttcatccgc aacgcgtacg gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa
     7141 tgcggatcgt agtgccagta ccctgcttcg actggatttc gacatgaccg cccatctcct
     7201 ggatattacg tttaacgacg tccatgccga cgccgcgccc ggagacgtcg gtgacctgct
     7261 ctgccgtgga gaagcctggt gcaaatatca gcattgcaac ttcgtcgtcg ctcatgtttt
     7321 cgctgacagt caaaccttgc gaggccgctt ttgccagaat tcgctcacgg tttagccccg
     7381 ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg atgttcggca gacagaatta
     7441 aatttccgac gctgttttta cctgcggcga gccgtttttc tggcagttca ataccgtgat
     7501 cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat gcgttctatc aggctcttgt
     7561 cgagctcggt ggaactgccc accagcgtca gttctacctg cttgccgagt tttcccgcca
     7621 gatcacgcac cagccgggga tagcgactaa agacatattc catcggcatc atgcgaatcg
     7681 acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca
     7741 aatcaccatg attaaccggg tccagttcgc tggaacgctg ggcaagcatg gactgggtga
     7801 taaccagctc gccgacgagg ttaattaatt gatcaacctt ttctaccgct acacggatgc
     7861 tggtggattc actgctgcgc gtcgtttttt cccgctccac gcggccggtt ggcgcttgtt
     7921 cggctgccag tttaagcaca ggtggggtag ctatttttgg cgaaacttct actgtttcaa
     7981 aggtaatctg atcggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga
     8041 tatcgcccgg taatattgcc gagagagaat ccgccccttt caccacgtca tttaacgttg
     8101 tcagatgccc cagctcttct tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga
     8161 taattcgtcg cggcaactga ctgcgacttt gctcatcttg cggttcactt ttggcaacca
     8221 cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc ttctaatgcc agttgacgca
     8281 aggcctggca gatataatcg tagctggcgg catccggctc ttgcgactgt ttataagcgt
     8341 cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga
     8401 gttgcatctc acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct
     8461 gcaaaacgct gaagccaaaa gttcctgccc ctcctttgat cgagtgggca gcccgaaaga
     8521 tggcattcaa ttgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca
     8581 tatcagccaa cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcga
     8641 tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg gaacactgac ctgtggtgca
     8701 acctcaggtt tttctagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa
     8761 atggcctgtt cggcttgttt gttcagtacc aacaggctga tgcgacggtt gacggcatca
     8821 tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc cgacgacacg taacactttg
     8881 ccgctatcca accctccgac catcagttcg cggcgggatg cattggcccg atcggcagaa
     8941 agctcccagt tgctgtaacc tttctcaccg ctggcgtagg gaaaatcatc ggtatgacct
     9001 gaaaggctaa tacggttggg aataccgttc agcacaggcg cgatggcgcg cagaatgtcg
     9061 cgcatatagg gttcgacatc ggcactgccg gttctaaaca tcgggcgatt ctggctatcg
     9121 atgatctgaa tacgtagacc ttcctggacc agatcgattt tgagatggag acgtaacgcc
     9181 cgcagtttcg gatcggactc tatcaactgg tcgagatcac cccgcaattt ccgcaatcga
     9241 ctttgctcca tgcgtttttt cagctcttcg atgttcggct gcttattcac ttccccctgg
     9301 ctttgggtgt aatcatcacc accgccggga attgggcttt cactattgga aatgcgatcg
     9361 ccgcccgtaa ccgctgtcgc cagtggagtc cggaagtact ccgcaatctg aatcaactct
     9421 tttgggctgg agatggagat cagccacatc accagaaaaa aggccatcat cgcagtcata
     9481 aagtcggcat aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt
     9541 cgtttgacga caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt
     9601 gttgcggatt tttcaccgca cgcacatgct cttccagttc aataaacgac ggacgttcgc
     9661 tggaatagag cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat
     9721 tagaaagcag agtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac
     9781 gtaaaacact cgctagtggg gaaataaatc cgtaagccaa taaaataccg aggaaagtcc
     9841 ccaccatcgc atgtgcgata agcgccccca gctcggcggc aggacgatcg gctgaaccta
     9901 acgcgtgaac gccccccatt acagccgcga caataccaaa cgccggaagt gagtccccga
     9961 ccagcgccag actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat
    10021 ccatcagggc ttcgatttcg aaggtgttca tgtgaccgct gataatcagg cgcagataat
    10081 cgacgataaa atcaagcatg acgctatccg cgaggatgcg tgggtagctg gcgaagatct
    10141 cgctctcacg gggattttca atatcacgtt ccagcgaaaa catccccatc tgccgcgatt
    10201 tcgccatcaa ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg
    10261 agcgacgaaa cagcaacggc agcgctttca gcgtgccttt aatcgctttg ccattattgc
    10321 cgacgataaa cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga
    10381 gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca
    10441 ggtaacctaa taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc
    10501 agtcgtcagg cgttaacgcg cgattgggga aaaaaaaagc agcggtacgt cgttaccgct
    10561 gctggaatgt tgcgccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca
    10621 gttgtgggat aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg
    10681 gcggttgaca taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat
    10741 tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca
    10801 atgtccaggc acgggttaat gccagcagtg gtccttcttc tgcttgtggg cactgttcaa
    10861 ggtataaacg gtaggctttg atcaccgcat cgacgccatt acacaaaccg gttttcagta
    10921 aaaactgcca tgcattacag aacatcgaag catgaacgtt ttgttcccag gtcataaacc
    10981 agtcggttga gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataaa
    11041 gttttatcag gcgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc
    11101 ccagggtgat caattccatt gccagctgaa tatcccgcgc ttcctgaaca atgctttttt
    11161 cactcatgat caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag
    11221 caagcgtgtt gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc
    11281 ttgcgtcaac tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagctgatt
    11341 ggtttctgcc agcttaacca tttgcg
//
LOCUS       DAJTRM010000114        11202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRM010000114 DAJTRM010000000
VERSION     DAJTRM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.114"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..460)
                     /locus_tag="OO322_003428"
     CDS             complement(<1..460)
                     /locus_tag="OO322_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5908177.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKN"
     gene            complement(572..1600)
                     /gene="malI"
                     /locus_tag="OO322_003429"
     CDS             complement(572..1600)
                     /gene="malI"
                     /locus_tag="OO322_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5908178.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1775..3367
                     /gene="malX"
                     /locus_tag="OO322_003430"
     CDS             1775..3367
                     /gene="malX"
                     /locus_tag="OO322_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5908179.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3377..4549
                     /gene="malY"
                     /locus_tag="OO322_003431"
     CDS             3377..4549
                     /gene="malY"
                     /locus_tag="OO322_003431"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5908180.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4653..5654
                     /gene="add"
                     /locus_tag="OO322_003432"
     CDS             4653..5654
                     /gene="add"
                     /locus_tag="OO322_003432"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5908181.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5690..6730)
                     /locus_tag="OO322_003433"
     CDS             complement(5690..6730)
                     /locus_tag="OO322_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5908182.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6973..7098
                     /gene="blr"
                     /locus_tag="OO322_003434"
     CDS             6973..7098
                     /gene="blr"
                     /locus_tag="OO322_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5908183.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7371..7586
                     /gene="ydgT"
                     /locus_tag="OO322_003435"
     CDS             7371..7586
                     /gene="ydgT"
                     /locus_tag="OO322_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5908184.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7672..8112
                     /locus_tag="OO322_003436"
     CDS             7672..8112
                     /locus_tag="OO322_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5908185.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8189..8770
                     /gene="rsxA"
                     /locus_tag="OO322_003437"
     CDS             8189..8770
                     /gene="rsxA"
                     /locus_tag="OO322_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5908186.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8770..9348
                     /gene="rsxB"
                     /locus_tag="OO322_003438"
     CDS             8770..9348
                     /gene="rsxB"
                     /locus_tag="OO322_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5908187.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9341..>11202
                     /gene="rsxC"
                     /locus_tag="OO322_003439"
     CDS             9341..>11202
                     /gene="rsxC"
                     /locus_tag="OO322_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5908188.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2613 a         2894 c         2832 g         2863 t
ORIGIN      
        1 tatttttatt ttctgccgcc atagaagtga tggtcagaat aacgccacct ccattttttt
       61 ccatttctgg cgcaacaagt tgtgacagat ggaaaaaaga aaacacattc agttcataag
      121 cacggcgaaa atctgccatt ggcatatcaa acggtttagg accaccgcca ccggcgttgt
      181 taaccaggat atcaacttta cccagattac tgacggcaaa gtctgccagt gcagagagtt
      241 cctgttcgga agtaatatca caacgccagg caaatgcctg accatccagt tgttgaattt
      301 cgtctacaac atggttagct gcgtcggcgt taatatcact gaccaccaca gatgcgccag
      361 ctgtcgcgaa tgtaatggcg atttctttac caatacctgc acccgcacct gtgatgatgg
      421 cgcattttcc gtcgagtctc aggttgtcag aattaaacac ataaacctcc tgtgaacttc
      481 agtaaaaata aaaggcaccg cctgtgtgcc acatttttta tcattaacct tgaggaaagg
      541 acactaccaa gtatagcaga taaagagagg attatttcgc cgcaatgagc cgggcgggaa
      601 taataagatt gcgtgaatgc gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa
      661 gtgtgatacc aagttcccgc gctggcgtgc tggcccaggt aacaggtata tcatcaagtg
      721 tggttggtgt cgcatcggta aatgccgcca gcgaaacctg ttgctcaaag taacgatcga
      781 ctccgctttc cccgctttgc cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa
      841 tagtttcgtt atagcaaacc acggcactga tggtcgggtt atgacgtaaa agcgccgtga
      901 tagcttccgc ggcttgcttc tggctggaag tgcactccaa cacccaatcg ctgtgaaacg
      961 gcaggccaaa ttttagtaga gttgcacaat agccccccac ccgttctgca cgggttaatg
     1021 aggaactttg ccctcccagc caggcgatcc gctgatgccc attgcgaatg agatgctccg
     1081 tcaacaactg tgcagcctgc atgttgtccg ggcgaaccgt atcaacatca tcaagataac
     1141 tggcacggga agcgaaaatc accgggatag ctttttcttc tgccatccgc cgcaggtcat
     1201 cgctgcttcc tgcagccccg gcaattacca caccatcgac accctgattc agtaacagtg
     1261 aaaaccgctg tgccagctgc tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc
     1321 cctgtgcttc cagagcttcc gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata
     1381 aatcacggac gatcaaacca atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc
     1441 gattgcgcac aaatcccagc tcttcaatgg cggcgttaac gcgttctcct gtggcggtag
     1501 agattcgccc tttgccatta agcaccagcg aaacggtact taccgacacg cccgcagcca
     1561 gcgcaacatc atgaatggtt atttttttgg cggtagccat gcagatgacc tactccctga
     1621 ttatgtgaca gataaaacgt tttacctttt atttatctta tacccgctat tatcgttgcg
     1681 taatgtgatt tatgactcac taaaatttga taaaacgttt tatcttctcg cgcaatttac
     1741 tgaatccaga ttgttctcta cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt
     1801 cacgctgtgg gagttcttcc agcagttagg caaaaccttc atgttacccg tggcattatt
     1861 gtcgttctgc ggcattatgc tcggcattgg tagttctctt agcagccatg atgtcataac
     1921 cctgatcccg gtcctgggca accccgtgtt gcaggctatc tttacctgga tgagtaagat
     1981 tggctcgttt gcttttagtt tcctgcctgt catgttctgt atcgccatcc cgctgggcct
     2041 ggcacgcgaa aataaaggcg tagcggcatt cgctggcttc gtcggttatg cggtaatgaa
     2101 cctcgcggta aacttctggt tgaccaataa aggcattctg ccaaccacag atgccgccgt
     2161 tctgaaagcc aataacatcc agagcattct tgggatccag tcgatcgata ccgggatcct
     2221 cggtgcggtg atcgccggta ttatcgtctg gatgctgcat gagcgtttcc ataatatccg
     2281 cctgccggat gcgctggcat tcttcggcgg tacgcgcttc gtaccaatta tctcctcgct
     2341 ggtgatgggc cttgtcggcc tggtgattcc attagtctgg ccgattttcg ccatgggtat
     2401 tagcggcttg ggccatatga taaacagcgc gggtgatttc ggaccgatgc tgtttggtac
     2461 cggtgaacgt ctgctgttgc cgtttggtct gcatcacatt ctggtggcat taattcgctt
     2521 taccgacgca ggcggcacgc aggaggtctg cggtcaaacc gtcagcggcg cactgaccat
     2581 cttccaggcg caattgagtt gcccgaccac tcacggtttt tctgaaagcg tcacgcgttt
     2641 cctttcgcaa ggtaaaatgc ctgcgtttct cggcggtcta ccaggtgcag cgttagctat
     2701 gtatcactgc gcgcgcccgg aaaatcgcca taaaattaaa ggtctgctga tttctggcct
     2761 gatcgcctgc gtcgttggcg gcactaccga accgctggaa ttcctgttcc tgttcgtagc
     2821 gccagttctg tatgtcatcc acgcgctgtt aaccggcctc ggcttcaccg tcatgtctgt
     2881 gctcggcgtc accatcggta ataccgacgg caatatcatc gacttcgtgg tgttcggtat
     2941 tttgcatggt ctgtcaacca agtggtacat ggtgccagtg gtggcggcaa tctggtttgt
     3001 cgtttactac gtcatcttcc gtttcgctat cacccgcttc aatctgaaaa ccccggggcg
     3061 cgatagcgaa gttgccagct caatcgaaaa agccgttgcc ggtgcgccgg gtaaatcagg
     3121 ttacaacgtt cctgcaatcc tcgaagcatt aggcggtgcc gacaatattg tcagcctcga
     3181 taactgcatt acccgtctgc gtttgtctgt ggaagatatg tcgcttgtta atgtgcaggc
     3241 actgaaggac aatcgggcaa ttggcgtagt acaacttaat caacataacc tgcaggttgt
     3301 tatcgggcca caagttcagt cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca
     3361 ggcataagga taagatatgt tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg
     3421 tacacagtgg gattatgtcg ctgaccgttt cggcactgct gacctgttac cgttcacgat
     3481 ttcagacatg gattttgcca ctgccccctg cattatcgag gcgctgaatc agcgcctgat
     3541 gcacggcgta tttggctaca gccgctggaa aaacgatgag tttctcgcgg ctattgccca
     3601 ctggttttcc acccagcatt acaccgcaat tgatacgcag acggtggtgt atggcccttc
     3661 tgtcatctat atggtttcag aactgattcg tcagtggtct gaaacaggtg aaggcgtggt
     3721 gatccacacg cccgcctatg acgcatttta caaggccatt gaaggtaacc agcgcacagt
     3781 aatgcccgtt gctttagaga agcaggctga cggctggttt tgcgatatgg gcaagctgga
     3841 agccgttttg gcgaaaccag aatgtaaaat tatgctcctg tgtaacccac agaatcctac
     3901 cgggaaagtg tggacgtgcg atgagctgga gatcatggct gacctgtgcg agcgtcatga
     3961 tgtgcgggtt atttccgatg aaatccatat ggatatggtc tggggcgagc agccgcatat
     4021 tccctggagt aatgtggcgc ggggagactg ggcgttgctg acatcgggat cgaaaagttt
     4081 caatattccc gcgctgaccg gtgcttacgg gattatagaa aatagcagca gccgtgatgc
     4141 ctatttatcg gcactgaaag gacgtgatgg gctttcttcc ccttcggtac tggcgttaac
     4201 tgcccatatc gccgcctatc agcaaggtgc accgtggctg gatgccttac gcgtctatct
     4261 gaaagataac ctgacgtata tctcagataa aatgaacgcc gcgtttcctg aactcaactg
     4321 gcagatcccg caatccactt atctggcctg gcttgatcta cgtccgttga atattgacga
     4381 caacgcgttg caaaaagcgc ttatcgagca agaaaaagtc gcgatcatgc cggggtatac
     4441 ctatggtgaa gaaggtcgtg gttttgtccg tctcaatgcc ggctgcccac gttcgaaact
     4501 ggaaaaaggt gtggctggat taataaacgc catccgcgct gttcgctaac cccaattgcg
     4561 caacgtaaaa aatcgttgcg caatcgtgga tttttaccct gctttgtttt tataatggtg
     4621 cgcactttta tatccagaaa aagagtgcga ccatgattga taccaccctg ccattaactg
     4681 atatccatcg ccaccttgat ggcaacattc gtccccagac cattcttgaa cttggccgcc
     4741 agtataatat ctcgcttcct gcacaatccc ttgaaacact gattccccac gttcaggtca
     4801 ttgccaacga acccgatctg gtgagctttc tgaccaaact tgactggggc gttaaagttc
     4861 tcgcctctct tgatgcctgc cgccgcgtgg catttgaaaa cattgaagat gcagcccgta
     4921 acggcctgcg ctatgtcgag ctgcgttttt caccaggcta catggcaatg gcacataagc
     4981 tgcctgtagc gggtgttgtc gaagcggtga tcgatggcgt acgtgaaggt tgccgcacct
     5041 ttggtgtgca ggcgaagctt atcggcatta tgagccgaac cttcggcgaa gccgcctgtc
     5101 agcaagagct ggaggccttt ttagcccacc gtgaccagat taccgcactt gatttagctg
     5161 gtgatgaact tggattcccg ggaagtctgt tcctttctca cttcaaccgc gcgcgtgatg
     5221 cgggctggca tattaccgtc catgcaggcg aagctgccgg gccggaaagc atctggcagg
     5281 cgattcgtga actgggtgcg gagcgtattg gacatggcgt aaaagccatt gaagatcggg
     5341 cgctgatgga ttttctcgcc gagcaacaaa ttggtattga atcctgtctg acctccaata
     5401 ttcagaccag caccgtagca gagctggctg cacatccgct gaaaacgttc cttgagcatg
     5461 gcattcgtgc cagcattaac actgacgatc ccggcgtaca gggagtggat atcattcacg
     5521 aatataccgt tgccgcgcca gctgctgggt tatcccgtga gcaaatccgc caggcacaga
     5581 ttaatggtct ggaaatggct ttcctcagcg cagaggaaaa acgcgcactg cgagaaaaag
     5641 tcgccgcgaa gtaacaaaaa tggatggtgc aaattgcacc atccattttt cacgcaaggc
     5701 acaacgtcgc gcgatgtttg gcggattcga tgcccagctc aatcaactcc attacctgga
     5761 ttgcctggct tgccggaacc ggattttcac catcgccatt taacgcatca cgaatcgccg
     5821 catagtaagc cggataattc ccaggcaccg tcaacagcgt ttcttcgaca cgttcctcac
     5881 cttccacgcg ggtaagtacg ccatcacgca tatcgtagcc ccagtcttcc tgcggcagac
     5941 gctcgccatt tttcagacgt tcttcctgtg gatcgaggcc atatttcaca taactgcctc
     6001 gggatccatg cacgatatac cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa
     6061 tgactcgccg ctgtggatag gacaagatgg cgtggaaata atcggtcgac tgcgctccgg
     6121 gccgtaactg tgccaaatct accgtcatgc tgaccggtaa accaaatagc gtaatggcct
     6181 gatcaagaag atgcggtgct aaatcgtacc agataccgct gcctgggccg ccctgttcac
     6241 gccaacgatc gcgcacctgc ggacggaagc ggtcaaaatg agactcaaag taagcaactt
     6301 cacccagcac gccttccgcg agcaaacctt ttagcgtcaa gaaatcgcta tcccaacggc
     6361 ggttatggaa tacagacagc acacgcccca ggctttttgc cagcgcatcc agctctcgcg
     6421 cttgtgacag tgtcacggta aagggtttat caacgaccac atgtttaccc gcctcaagcg
     6481 ccgctttggc taacgggaaa tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt
     6541 tgggatcgtt aaacagatgc ttcggctcag agacaaccgt aaccgttggc cagtcggctt
     6601 ttacttttgt ttcatcactg ctggagatta ccgccagttc cagcccgggc gtgcccgcaa
     6661 tcaggggcgc atggaaggtt ttgctcgcat aaccataccc aatcaaccca acacggatgt
     6721 tgtcgctcat gtcattgcct ctcattgaag tatgatggct atttgacact atcctttacc
     6781 cacgctcaac agtttaataa cctgccagca ataagggatg ttgtttaact taagtcaaaa
     6841 aaatagcgaa ttttccaacg acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg
     6901 ctggagctat tattgctaag taacatttac cccctgaagt taatggatca atcaagagag
     6961 atgtgggctg taatgaatcg tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca
     7021 gtcattcttc ttggcgtggc gagtcacatt gacaactatc agccacctga acagagtgct
     7081 tcggtacaac acaagtaagc tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat
     7141 gttttcactt gtcggatatt aaaccaggaa tttattatct tgttcgatgt tgttggtgat
     7201 tgtcagggat agtaaagcgc gttactcttt tcagagcagt ttttcatccc tgaatatttg
     7261 ccatcagcgc attcttatta ttgtaataac acaagcgttc acttttggtg aaattttatc
     7321 atcactaata atacctaccc ctttggtatt aattatttta tggacctttt atgactgttc
     7381 aggactactt attaaaattt cgcaaaatca gttcactcga aagtctggaa aaactctacg
     7441 accatcttaa ttacaccctg acggacgatc aggaactgat caatatgtat cgtgctgccg
     7501 atcaccgtcg cgcagagctg gtttctggcg ggcgtttgtt tgacctcggc caggtaccga
     7561 agtccgtctg gcactatgtc caataaagaa agtagcgatc ggttataaaa gccttataat
     7621 aacgccccta aaattagatt tgcctgtttg cgtcgctatt ctggagaacc tatgaccacc
     7681 acaacgccac aacgtattgg aggctggttg cttggcccgt tggcctggct tttagtcgct
     7741 ctgttgagta cgacgctggc gttgttgctg tataccgctg cgttatcttc tccgcaaaca
     7801 tttcaaacgc tcggcggaca agcactgacg acacaaatct tatggggcgt ttcttttatt
     7861 accgctatcg ccatgtggta ttacacactg tggttgacca tcgctttctt taaacgtcgg
     7921 cgttgtgttc ctaaacacta tattatctgg ctgcttattt ccgtattgct ggcagtaaaa
     7981 gcattcgctt tttcacccgt ggaagttggc atagccgttc gccagttgct gtttactttg
     8041 ttggcaaccg cattgatcgt cccctatttc aaacgctcgt cgcgggtaaa agcgacgttt
     8101 gtgaatccgt aataacctta cagttaacct gttgtcgcct gctctggatt aacggataat
     8161 aggcggcttt tttatttcag gccgaaaaat gactgactac ctgttactgt ttgtcggaac
     8221 tgtactggtc aataactttg tactggtcaa gtttctcggt ctctgtccgt ttatgggggt
     8281 ttccaaaaaa ctggaaaccg cgatgggcat ggggctggca acaacgtttg tgatgacgct
     8341 gacgtctatt tgcgcctggc ttatcgatac gtggattttg atcccactta atctgattta
     8401 cctgcgcacc ctggcattta ttctggtgat tgctgtggtc gtgcagttca ccgagatggt
     8461 ggtgcgcaaa accagcccgg tgctttaccg tctgctgggg atttttttgc cgcttatcac
     8521 caccaactgt gcagtgctcg gcgtggcgtt gctgaatatc aatctcgggc acaatttctt
     8581 gcagtcggcg ctgtacggtt tttccgccgc tgtcggtttc tcgctggtga tggtgctctt
     8641 cgccgccatc cgcgaacgcc ttgctgtggc tgatgtcccg gcaccttttc gcggtaatgc
     8701 cattgcgtta attaccgcag gtcttatgtc tctggccttt atgggcttta gtggtttggt
     8761 gaagttgtaa tgaatgctat ctggattgcc gttgccgccg tgagcctgct gggcctggcg
     8821 tttggcgcca ttctgggtta tgcctcccgc cgttttgcgg tggaagacga tccggtcgtt
     8881 gagaaaattg acgaaatctt accgcagagc cagtgtggtc agtgcggtta tcccggctgt
     8941 cgcccctacg cggaagccat cagctgtaac ggtgaaaaaa tcaaccgttg cgccccaggt
     9001 ggcgaagctg tgatgctaaa aattgccgag ttgcttaatg tcgagccgca gccgctggat
     9061 ggcgaagcgc aagagctaac gcctgcgcgg atggtggcgg ttattgatga aaataactgt
     9121 attggctgca ctaaatgtat tcaggcttgt ccggtagacg ccatcgttgg cgctacccgt
     9181 gtcatgcata cggtaatgag tgatctctgt acgggctgca atttatgtgt tgatccgtgc
     9241 ccgacgcact gcatctcgtt gcaaccggtc gcagaaacac ctgactcctg gaaatgggat
     9301 ctgaacacca ttcccgtgcg tatcattccc gtggaacacc atgcttaagt tattctctgc
     9361 attcagaaaa aataaaatct gggatttcaa cggcggcatc catccaccgg agatgaaaac
     9421 ccagaccaac ggtacccccc tgcgccaggt actactggcg cagcattttg ttattccact
     9481 gaaacagcat attggcgctg aaggtgagtt gtgcgttagc gtcggcgata aagtattgcg
     9541 cggccagccg cttacccgtg gtcgcggcaa aatgctgcct gttcacgcgc ccacctcggg
     9601 taccgttacg gctattgcgc cccactctac ggctcatcct tcagctttag ctgaattaag
     9661 cgtgattatt gatgccgatg gtgaagactg ctggatcccg cgcgacggct gggtcgatta
     9721 tcgctctcgc agtcgcgaag agttaatcga gcgcatacat cagtttggtg ttgccgggct
     9781 gggcggtgcc ggattcccga caggcgttaa attgcagggt ggtggagata agattgaaac
     9841 gttgattatc aacgctgctg agtgcgagcc gtacattacc gccgatgacc gtttgatgca
     9901 ggattgcgcg gctcaggtcg tagagggtat tcgcattctt gcgcatattc tgcagccacg
     9961 cgaaattctt atcggcattg aagataacaa accgcaggcg atttccatgc tgcgcgcggt
    10021 gctggcggac tctaacgata tttctctgcg ggtgattcca accaaatatc cttctggcgg
    10081 tgctaaacaa ttaacctaca ttctgaccgg gaagcaggtt ccacatggcg ggcgttcatc
    10141 cgatatcggc gtattaatgc aaaacgtcgg cactgcttat gcagtgaaac gtgccgttat
    10201 tgatggcgag ccgattaccg agcgtgttgt aaccctgact ggcgaagcaa tcgctcgccc
    10261 gggcaacgtc tgggcacggc tggggacgcc agtgcgtcat ttattgaatg atgccggatt
    10321 ctgcccctct gccgatcaaa tggtgattat gggtggcccg ctaatgggct ttaccttgcc
    10381 atggctggat gtcccggtcg taaagattac caactgtctg ttggctccct ctgccaatga
    10441 acttggcgaa ccacaggaag aacaaagctg catccggtgt agcgcctgtg ctgacgcctg
    10501 ccctgtggat cttttgccgc aacagttgta ctggttcagc aaaggtcagc aacacgataa
    10561 agctaccacg cataacattg ccgactgcat tgaatgtggg gcttgcgcgt gggtttgccc
    10621 gagcaatatt cccttggtgc aatatttccg tcaggaaaaa gctgaaattg cggctattcg
    10681 tcaggaagaa aagcgcgccg cagaagccaa agcgcgtttc gaagcgcgcc aggctcgtct
    10741 ggagcgcgaa aaagcgactc gccttgaacg acataagagc gcagccgttc aacctgcagc
    10801 caaagataaa gatgcgattg ctgccgctct ggcgcgggtg aaagagaaac aggcccaggc
    10861 tacacagcct attgtgatta aagcgggcga acgcccggat aacagtgcaa ttattgcaga
    10921 acgggaagcc cgtaaagcgc aagccagagc gaaacaggca gaactggagc aaactaacga
    10981 cgcagcaacc gttgctgaac cacgtaaaac tgccgttgaa gcagctatcg cccgctccaa
    11041 agcgcgcaag ctggaacagc aacaggctaa tgcggaacca gaagaacagg tcgatccgcg
    11101 caaagccgcc gtcgaagccg ctattgcccg tgccaaagcg cgcaagctgg aacagcaaca
    11161 ggctaatgcg gaaccagaag aacaggtcga tccgcgcaaa gc
//
LOCUS       DAJTRM010000115        11082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRM010000115 DAJTRM010000000
VERSION     DAJTRM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.115"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            321..518
                     /locus_tag="OO322_003440"
     CDS             321..518
                     /locus_tag="OO322_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908189.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(468..1661)
                     /locus_tag="OO322_003441"
     CDS             complement(468..1661)
                     /locus_tag="OO322_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5908190.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1797..3521
                     /gene="iucA"
                     /locus_tag="OO322_003442"
     CDS             1797..3521
                     /gene="iucA"
                     /locus_tag="OO322_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5908191.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3522..4469
                     /gene="iucB"
                     /locus_tag="OO322_003443"
     CDS             3522..4469
                     /gene="iucB"
                     /locus_tag="OO322_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5908192.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4469..6211
                     /gene="iucC"
                     /locus_tag="OO322_003444"
     CDS             4469..6211
                     /gene="iucC"
                     /locus_tag="OO322_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5908193.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6208..7545
                     /gene="iucD"
                     /locus_tag="OO322_003445"
     CDS             6208..7545
                     /gene="iucD"
                     /locus_tag="OO322_003445"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5908194.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7551..9746
                     /gene="iutA"
                     /locus_tag="OO322_003446"
     CDS             7551..9746
                     /gene="iutA"
                     /locus_tag="OO322_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5908195.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10297..10440)
                     /locus_tag="OO322_003447"
     CDS             complement(10297..10440)
                     /locus_tag="OO322_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908196.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10679..>11082
                     /locus_tag="OO322_003448"
     CDS             10679..>11082
                     /locus_tag="OO322_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCR5908197.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYA"
BASE COUNT         2762 a         2793 c         2880 g         2647 t
ORIGIN      
        1 ggttcaggga tgaattaaaa accagtaaaa atacccgaca agaaatctgc aatgatttac
       61 ttcataaaaa acatgtcagt gaaaaagaaa cctggcgttg ggttgaagga tataataaag
      121 caacagaact ggctcgccaa tgccctgata cccatgtatt gtcaatttct gatcgtgaag
      181 gcgattttta tgacctgttt gaacgcgcag cgcagactcc cggaataaaa gctgactggc
      241 ttgtcagaat gaaatttaat aacagagcaa cgctgaatat taatggtaaa cgggatcata
      301 ggttgttgca cgaaagaatc atggagatta caccgcagca actggttgaa ttcactatac
      361 ctgatgggcg ggggcagcaa gccagaacac tgcctgacat gctttcatca gacgcaggtc
      421 ctctctcata tcctctgatt tactccagta aggtgatgat catatcttta tttgatagta
      481 cggtaatgcc agataaatac agacacaatg gtaaataata tagttgcaaa taatgctgcc
      541 ataatcagcg cgattgcgac acctgttttc gataccagcg gcaccagcaa agcgcacagt
      601 ccatagccaa gtgtgtgact ggtagcgagc cagcccgcgc ctgcgccatc agccagctta
      661 tcattcagta gaagttgata agcgggtgtt gccagagctg ctccaaatga cagcactgca
      721 cagcccgggt aaaacaacca tagctgttcg gagagcatga tagccagacc accactcatc
      781 aacactccgg cactcaggag cagggccacc ggagtcaggc gctgcggacg gagtaccccg
      841 aactgcgcga taagcgcagc tactgcggac agacccaaca gccaggccac ctgttggcta
      901 atggcggtgg tatccgtagc gaactggcga gtaagggcag gcgaaagtcc aagctgcatc
      961 atgctcaccg ccgcagccag taatattgcg caaagcagat aaggcagaca atcccgcttc
     1021 agactgacgc tcttgcactc cggtgtgggc tgtggtggtg tgccgggcaa ccgcagcagc
     1081 atcagcagcg ccagcactgg ggctgccatc agcagcccca gtggcgccag aggatgaatg
     1141 gccagcattg ctgccgcgca cagcggcccg aatagcctgc cgcaactcag gccggagctg
     1201 atggttgcca gagcggccat gcgattccct tctccagctc tctgcaatgc ccagacctga
     1261 caggctggca ccattgccga cacggtcagc ccgtaggcga tccgggcgat gattaatatt
     1321 cccagtccca ccgctgttgt tatggcgctg gtagccatca gcacgcttcc cagccctagc
     1381 agggtaaagc ttatgccata tcctcccaga gcccacaaca ccacaggttt actgccagta
     1441 cgggaaattt gctttcccca ccacggggac gatggcagaa acagcattga gccaagcatc
     1501 agcagggcag cccatacaga cagactcaga tttgtctgta tgaccagaac agggagcaca
     1561 accagcagac cgttctgtcc gataccgaga agcccggcac tgaacgcaag tggccagcag
     1621 gacagtggtt tttggggcgt atcttcgatc cctggtgaca ttataatgtt tcaactccat
     1681 gtattaattg tgtttatttg taaaattaat ttatctgaca ataacattta ttgttgataa
     1741 tgagaatcat tattgacata attgttatta ttttactgtg tgggagctgt ttgactatga
     1801 ccctgccctc tgaaaaacca gccacagatg tggctgcgca gtgcttcctg aatgcactga
     1861 ttcgtgaaac cacagactgg aaactgacag aatacccgcc agacgaattg ctcatcccgc
     1921 tggatgagca gaaatcgctc catttcagag tggcttattt ctccccaacc cagcatcacc
     1981 gctttgcatt tcctgcacgt ctggtcacgg catcaggcag ttatcctgtc gactttacca
     2041 ctctctcccg gcttattatt gataagctac gccatcaact gtttctgccc gttcccctct
     2101 gcgaaacttt ccaccagcgc gtgctggaaa gccatgtcca tacgcaacag gcaattgatg
     2161 cccgtcatga ctgggccgct ctgcgtgaaa aagcgttgaa ttttggcgag gctgagcagg
     2221 cgctgctgac aggacacgct ttccaccctg cgcctaagtc tcatgaaccg tttaaccggc
     2281 gggaggctga acgctacctg cctgatatgg caccccactt cccactgcgg tggttttcgg
     2341 tggataaaac gcaaatcgcc ggtgaaagtt tgcatctcaa ccttcaacag cggctgacgc
     2401 gatttgccgc agagaatgcg cctcagttac tcaacgaatt aagtgacaac caatggctgt
     2461 tcccgttgca cccgtggcag ggggaatatc ttttgcagca ggggtggtgc caggcacttg
     2521 ttgctaaagg gctgattaaa gacttaggtg aggccggcac gtcgtggctg ccgaccacct
     2581 cttcccgttc cctctactgt gccaccagcc gcgatatgat caagttctcc ctgagcgtac
     2641 ggctgaccaa ctccatccgt actctgtccg tgaaagaagt gaagcgtggg atgcgcctgg
     2701 cacgcctggc tcaaaccgac ggctggcaga tgctacaggt ccgcttcccg actttccggg
     2761 taatgcagga ggatggctgg gccgggctgc tcgatcttaa cggcaacatc atgcaggaaa
     2821 gtctgtttgc cctgcgtgaa aatctgctgg tggatcagcc gaaaagccag actaacgtac
     2881 tggtctccct gactcaggcc gcacctgatg gcggtgattc gctgctggtt tcggcggtaa
     2941 aacgcctgag cgatcgcctc ggtatcactg tgcaacaggc cgcccatgca tgggttgatg
     3001 catactgtca gcaagtgcta aagccgctgt ttacggctga agcggattac ggcctggtgc
     3061 tgctggcgca tcagcaaaat attcttgtcc agatgcttgg ggatctgccg gtcggattta
     3121 tttaccgtga ctgtcagggc agcgctttta tgcctcacgc gacagactgg ctcgattcta
     3181 ttggcgaggc gcaggcggaa aatattttca cccatgagca gttgctgcgc tatttccctt
     3241 attacctgct ggttaactcc acttttgctg tgaccgctgc gctgggggct gccgggctgg
     3301 acagcgaatc gaatctgatg gctcgtgtac gagcatcgct ggctgaagtg cgtgatcagg
     3361 tgactcataa aacctgtctc aactacgtgc tggaaagtcc gtactggaac gtaaaaggta
     3421 actttttctg ttatctgaac gatcataacg agaacaccat tgttgaccct tcggtgatct
     3481 atttcgattt cgctaacccg ctgcaggctc aggaggtctg aatgtctgag gcaaacatta
     3541 ttcacagcag atatggactg cgctgtgaaa aactcgacaa gcccctgaat cttggctggg
     3601 gactggacaa tagcgcggtg ttgcactgtc ccggggagct gccgacaggg tggctgtgcg
     3661 atgcgctgga tcagatattt atcgccgcac cacaactgtc agcagtggct ctcccctggg
     3721 ccgaatggcg tgaggagcca caggcgctga cgctttttgg gcaggtaaaa agcgacatta
     3781 tccatcgtac tgccttctgg cagttaccgt tatggttgag ttcaccggca aaccgggcct
     3841 ccggtgaaat ggtttttgat gcagagcgtg agatttattt cccgcagcgc ccaccccgtc
     3901 cacagggtga ggtttaccgc cgttacgatc cacgtattcg caggatgctg agtttccgca
     3961 ttgccgatcc cgtttctgat gcagaacgtt tcacccgctg gatgaatgat ccgcgcgttg
     4021 agtatttctg ggagcaaagt ggctcactgg aggtacagac cgcctatctg gagcgccagc
     4081 tcaccggtaa acatgcattc ccgctgattg ggtgcttcga cgatcggcca ttcagctatt
     4141 tcgaaatcta ctgggcggcg gaagaccgca ttggtcgtca ctattcgtgg cagccctttg
     4201 accgtggcct gcatctgctg gttggtgaac agcaatggcg cggggcccac tatgtgcaaa
     4261 gctggctgcg cgggctgaca cattacctgc tgctggatga gccccgtacg cagcgcacag
     4321 tactggagcc acgcaccgat aaccagcgcc tgttccgcca tcttgagcct gcgggatacc
     4381 ggacaattaa agagttcgac ttcccacata agcgctcgcg catggtgatg gcggatcgtc
     4441 atcacttctt cacggaggtc ggtctgtaat gaatcacaag gactgggatt ttgttaaccg
     4501 tcggctggtg gcaaaaatgt tgtctgagat ggagtatgag caggttttcc acgcggaatc
     4561 tcagggcgat gaccactact gcattaacct gccgggagca caatggcgct tcatcgctga
     4621 acgtggtatc tggggctggc tgtggattga tgcccaaacg ttgcgctgca cggacgaacc
     4681 agtactggcg cagacgctgc tgatgcagct aaagccggtg ctgtcaatga gtgatgccac
     4741 tgttgcagag catatgcagg atttgtatgc cacgctactg ggcgacctgc aactgctgaa
     4801 ggcccgtcgc ggactgagtg ccagtgacct gattaatctt gatgccgatc gtctgcaatg
     4861 cctgctgagc ggtcatccta aattcgtttt taataaaggt cgccgtggct ggggcaaaga
     4921 ggcgctggaa cgatatgcgc cagagtatac caacaccttc agactgcact ggctggcggt
     4981 aaaacgtgaa catatgatct ggcgctgcga taacgatctg gatattcagc agttgttgac
     5041 tgccgcaatg gatccgcagg agtttacacg cttcagtcag gtctggcagg aaaacgggct
     5101 ggatcacaac tggctgccgc tgccggtaca tccgtggcag tggcagcaaa aaatcgccac
     5161 agacttcatc gctgattttg ccgaaggcag gatggtgtct ctcggcgagt ttggcgacca
     5221 gtggctcgcc cagcagtcgc tgcgtaccct gaccaacgcc agccgccggg gagggctgga
     5281 tatcaagctg ccgctgacca tctacaatac ctcatgctac cgggggatcc ctggcagata
     5341 tatcgctgcc ggaccactgg cttcacgctg gctgcaacag gtttttgcga ccgacgccac
     5401 cctggtgcaa agcggcgcag tgatccttgg tgaaccggct gcaggctatg tgtcccatga
     5461 aggctatgcc gcgcttgccc gggctcccta tcgctaccag gaaatgcttg gtgttatctg
     5521 gcgggagaat ccgtgccgct ggctgaaacc ggatgaaagt cctgttctga tggcaacact
     5581 gatggagtgc gacgaaaaca atcagccgct ggcaggcgca tatatcgacc gctccggact
     5641 ggacgctgaa acctggctta cgcaattgtt ccgggtggtt gtggttccgc tgtatcacct
     5701 gctttgccgc tacggtgtcg cgcttattgc acacggacaa aatataacgc tcgccatgaa
     5761 agagggggta ccacagcgtg ttctgctgaa agacttccag ggcgatatgc ggctggtgaa
     5821 agaagcgttt cccgaaatgg actctttgcc tcaggaggtg cgtgatgtca catcccgtct
     5881 gagtgcggac tacttaatcc atgatttgca gacgggtcac ttcgtgacag tactgcgttt
     5941 tatttcgcca ctgatggttc gtcttggcgt acctgaaagg cgattttatc aactgctggc
     6001 agcagtgttg agtgattaca tgaacaaaca tccacaaatg gcagagcgtt ttgcgctttt
     6061 ctcactcttc aggccacaaa tcattcgcgt ggtgctgaac ccggtaaaac tgacctggcc
     6121 ggacctggat ggcggcagtc gcatgctgcc gaattacctt gagaatctgc aaaatccgct
     6181 gtggctggta actcaggaat atgaatcatg aaaaaaagtg tcgattttat tggtgtcggg
     6241 acagggccat ttaatctcag cattgctgca ttgtcacatc agatcgaaga actgaactgt
     6301 ctcttctttg acgaacatcc tcatttttcc tggcatccgg gtatgctggt accggattgt
     6361 catatgcaga ccgtctttct gaaagatctg gtcagtgcag ttgcacctac aaatccctac
     6421 agctttgtta actatctggt gaagcacaaa aagttctatc gcttccttac aagcaggcta
     6481 cgtacagtat cccgtgaaga gttttctgac tatctccgct gggctgctga agatatgaat
     6541 aacctgtatt tcagtcatac cgttgaaaat attgatttcg acaaaaaaag tcgattgttt
     6601 ctggttcaga ccagtcgggg agaatatttt gcccacaata tctgccttgg tacaggaaaa
     6661 caaccttatt taccaccctg tgtgaagcat gtgacacaat cctgtttcca tgccagtgaa
     6721 atgaatcttc gtcggccgga cctgagtgga aaacggataa ccgtggttgg tggaggacag
     6781 agtggtgcag acctgttcct taatgcatta cgcggggaat ggggagaagc ggcggaaata
     6841 aactgggtct cacggcgtaa taattttaac gcactggatg aggctgcttt tgctgatgag
     6901 tattttacac ctgaatatat ttcaggcttc tccggactga aggaagatat tcgccatcag
     6961 ttactggatg agcagaaaat gacatcggat ggcatcactg ccgattcttt actgaccatt
     7021 tatcgtgagt tgtaccaccg ttttgaagtt ctgagaaaac caagaaatat ccgtctgcta
     7081 cccagtcgct cggtaacaac tctggaaagt agtggtccgg gctggaggtt attgatggaa
     7141 catcatctgg atcagggcag agagagcctg gaaagtgatg tggtgatttt cgccacaggt
     7201 taccgttctg cattgccaca aatacttccc tcactgatgc ccttgatcac catgcacgat
     7261 aagaacacct ttaaagtgcg tgatgacttc actctggaat ggagtggccc gaaagaaaat
     7321 aacatctttg cggtcaacgc cagcatgcaa actcatggca tcgccgaacc ccagctcagc
     7381 ctgatggcct ggcgatctgc acgtattctt aatcgcgtac tggggcgtga tttattcgat
     7441 ctcagtatgc cgcccgcact gattcagtgg cgcagcggca gccggaaaaa accacagccg
     7501 gaggctgctt ctttaactca ctatacagca aatattcagg aataacgatg atgcgcaaaa
     7561 agtatatgcc ccgggctctt ggtccgctgc ttcttgtcgt gctgtcacca gctgtcgccc
     7621 agcaaaacga tgataatgag atcatagtgt ctgccagccg cagcaatcga actgtagcgg
     7681 agatggcgca aaccacctgg gttatcgaaa atgccgaact ggagcagcag attcagggcg
     7741 gtaaagagct gaaagacgca ctggctcagt taatccccgg ccttgatgtc agcagccaga
     7801 gtcgaaccaa ctacggtatg aacatgcgtg gccgcccgct ggttgtcctg attgacggtg
     7861 tgcgcctcaa ctcttcacgt tccgacagcc gacaactgga ctctgtcgat ccttttaata
     7921 tcgaccatat tgaagtgatc tccggcgcga cggccctgta cggtggcggg agtaccggag
     7981 ggttgatcaa catcgtgacc aaaaaaggtc agccggaaac catgatggag tttgaggctg
     8041 gcacaaaaag tggctttaac agcagtaaag atcacgatga gcgcattgcc ggtgctgtct
     8101 ccggcggaaa tgaccatatc tccggacgtc tttccgtggc atatcagaaa tttggcggct
     8161 ggtttgacgg taacggcgat gccaccctgc ttgataacac ccagaccggc ctgcagcact
     8221 ccaatcggct ggacatcatg ggaaccggta cgctgaacat cgatgaatcc cggcagcttc
     8281 aactgataac gcagtactat aaaagtcagg gagacgacaa ttacgggctt aatctcggga
     8341 aaggcttttc cgccatcagc gggagcagca caccatacgt cagtaagggg ctgaattctg
     8401 accgcattcc cggcactgag cggcatttga tcagcctgca gtactctgac agtgatttcc
     8461 tgggacagga actggtcggt caggtttact accgcgatga gtcgttgcgg tactacccgt
     8521 tcccgacggt aaatgcgaat aaacaggcga cggctttctc ctcgtcacag caggataccg
     8581 accagtacgg catgaaactg actctgaaca gccaactgat ggacggctgg caaatcacct
     8641 gggggctgga tgctgagcat gagcgcttta cctccaacca gatgttcttc gatctggctc
     8701 aggcaagtgc ttccggaggg ctgaacaacc ataagattta caccaccggg cgctatccgt
     8761 catatgacat caccaatctg gcggccttcc tgcaatccag ctatgacatt aatgatattt
     8821 ttaccgttag cggtggcgta cgctatcagt atactgagaa cagggtagat gatttcatcg
     8881 actacacgca gcaacagaag attgctaccg ggaaggcgat atctgccgac gccattcctg
     8941 gtggttcggt agattacgat aactttctgt tcaatgctgg tctgctgatg cacatcaccg
     9001 aacgtcagca ggcatggttc aatttttccc agggggtggc attgccggat ccggggaaat
     9061 attatggtcg cggcatctat ggtgcagcag tgaacggcca tcttcccctg acaaagagcg
     9121 tgaacgtcag cgacagtaag ctggaaggcg tgaaagtcga ttcttatgaa ctgggctggc
     9181 gctttatcgg tgacaacctg cggactcaaa tcgcggcata ttactcgctt tccaataaga
     9241 gcgtggaaag gaataaagat ctgaccatca gtgtgaagga cgacaggcgc cgtatttacg
     9301 gcgtggaagg tgcggtggac tacctgatcc cggatactga ctggagtacc ggtgtgaact
     9361 tcaatgtgct gaaaaccgag tcgaaagtga acggtcaatg gcaaaaatat gacgtgaagg
     9421 aatcaagtcc atcgaaagcg acagcttaca ttaactgggc gccggaaccg tggagtctgc
     9481 gtgtacagag caccacttct ttcgacgtaa gcgatgcaga gggtaacgat attaatggtt
     9541 acactaccgt cgattttatc agtagttggc agcttccggt gggaacactc agcttcagcg
     9601 ttgagaacct cttcgaccgt gactatacca ctgtctgggg acagcgtgca cctctgtact
     9661 acagcccggg ttacggccct gcttcactgt acgactacaa aggccggggc cgaacctttg
     9721 gtctgaacta ctcagtgctg ttctgacggg gatgcttttg caacaagggt atgctgtaaa
     9781 caacatggtc cttggtggcc atgttgttta cagccagtat aatatacagc cttaacaaga
     9841 tattccgtct ccggaatggg ctgttcacca ctgaataaca ttatgtctgt tttatggatc
     9901 tttgactgaa atttatcgca tatcctcatt tcattgtccg tctgaaaact aaccttacaa
     9961 gtaagcgtcg tctcagcacc gtctggcaga tcctgaaatt cctgagagga tgatctgcgc
    10021 ccaatatcct gagccggaac tttgctgcaa tgaaggccaa cgaaaaatgg gggagtatgt
    10081 tatagagtac ttgctacagg gtaaaaagag gtacctgtcg ctggttctcc gttcagttta
    10141 accgggagat tttctcctgc gcctgtcata aagtccggta atggagattg ttaatacgat
    10201 ggtgcacgat gcatcgtcag tgcccggccc ggaggatgcc ccgtagctac atacggatca
    10261 gggttggcaa tatcgaatgg caggctatag gcaaagttaa agccccatgg agtagcacaa
    10321 aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tggagaactt cttcggcaca
    10381 ctgcaaaatc gcatcgtggt cgatcgctaa ttcgtcgtga atcactgaac gatgttccat
    10441 gcgaacagat attcgatcat atcgaaatat taaacattac ccctccccac attgggcaaa
    10501 aaaaaacaaa aaacgatcaa ttagtattga ttcacataat ctctcatcat taatgagaac
    10561 attgtcacat atttacaata tagaactgtt acttttagca agctgttagt aaccaaccgt
    10621 tactaataat tttaattctt aatactttgt tcattcaata aatgagttga gagaatatat
    10681 gaataaaata tactccctta aatatagtgc tgccactggc ggactcattg ctgtttctga
    10741 attagcgaaa agagtttctg gtaaaacaaa ccgaaaactt gtagcaacaa tgttgtctct
    10801 ggctgttgca ggtacagtaa atgcagcaaa tattgatata tcaaatgtat gggcgagaga
    10861 ctatcttgat cttgcacaaa ataaaggtat tttccagccc ggagcaacag acgtaacaat
    10921 cactttaaaa aacggagata aattctcttt ccataatctc tcaattccgg atttttctgg
    10981 tgcagcagcg agtggcgcag ctaccgcaat aggaggttct tatagtgtta ctgttgcaca
    11041 taacaaaaag aaccctcagg ccgcagaaac tcaggtttac gc
//
LOCUS       DAJTRM010000116        11075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRM010000116 DAJTRM010000000
VERSION     DAJTRM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.116"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..356
                     /locus_tag="OO322_003449"
     CDS             <1..356
                     /locus_tag="OO322_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908198.1"
                     /translation="DNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVV
                     KTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALH
                     HLRVQSTSKTAPQAK"
     gene            complement(559..912)
                     /locus_tag="OO322_003450"
     CDS             complement(559..912)
                     /locus_tag="OO322_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5908199.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1050..2696)
                     /gene="groL"
                     /locus_tag="OO322_003451"
     CDS             complement(1050..2696)
                     /gene="groL"
                     /locus_tag="OO322_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5908200.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2740..3033)
                     /locus_tag="OO322_003452"
     CDS             complement(2740..3033)
                     /locus_tag="OO322_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5908201.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3309..4565
                     /gene="yjeH"
                     /locus_tag="OO322_003453"
     CDS             3309..4565
                     /gene="yjeH"
                     /locus_tag="OO322_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5908202.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4581..5057)
                     /gene="fxsA"
                     /locus_tag="OO322_003454"
     CDS             complement(4581..5057)
                     /gene="fxsA"
                     /locus_tag="OO322_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5908203.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5394..6830
                     /gene="aspA"
                     /locus_tag="OO322_003455"
     CDS             5394..6830
                     /gene="aspA"
                     /locus_tag="OO322_003455"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5908204.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6948..8249
                     /gene="dcuA"
                     /locus_tag="OO322_003456"
     CDS             6948..8249
                     /gene="dcuA"
                     /locus_tag="OO322_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5908205.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8365..8703
                     /gene="cutA"
                     /locus_tag="OO322_003457"
     CDS             8365..8703
                     /gene="cutA"
                     /locus_tag="OO322_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5908206.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8679..10376
                     /gene="dsbD"
                     /locus_tag="OO322_003458"
     CDS             8679..10376
                     /gene="dsbD"
                     /locus_tag="OO322_003458"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5908207.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10413..10988
                     /locus_tag="OO322_003459"
     CDS             10413..10988
                     /locus_tag="OO322_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5908208.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2616 a         2904 c         2746 g         2809 t
ORIGIN      
        1 aggacaatgg caatctggaa tatgacacct attcccaacc ggaatggaaa cataacttgt
       61 tcgaccatta tctggcggtg ctttaccgct ttaaagatga aagtggcaaa gaacaattta
      121 gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc
      181 gccgaatgct tgatttcagc ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct
      241 atgttcgcac cgtagcggcg aataacgccc aaccgctaac ccaggatcaa tgcttacgcg
      301 cgctgcatca cttacgtgta caatccacca gcaaaactgc accacaggcg aaataatctc
      361 atctttgcta atgcgaggct ggagccatcc agcctgaaat gactgataac gtgatcgttg
      421 gtcatgccgg atgcggcgta aacgccttat ccggcctaca aattctcaag cattcaatca
      481 gttgcaatgc ctgctgtagg cctgataagc gaagcgcatc aggcaataat acgtttgtca
      541 cccagcctct ggatcacatc agttcgggca cttataaacc tgaccgataa tctggctatc
      601 cgtcgggaca aaactggaca acattccctg cgacgggctg ctgatgccat aaatcacgtt
      661 accgcccatt gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc
      721 ctcttctccg tgttgcccgg aaagccagtt gctttgctta cctgtcgcag taccaatcag
      781 ctggcactct gcgcctggct gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa
      841 ttcgttgctg gagctacaac ccgccatcag tagcgcggca ccgacaatcc ctgctaagta
      901 ttttacgtgc atgttattcc ccataatcaa tgagctggac gccactcgcg tcatccgtgt
      961 ctgaatctta tactagaaag atgaccaaaa gaaaaacccc cagacatttc tgcccggggg
     1021 tttgtttatt tctgcgaagt gcagggcaat tacatcatgc cgcccatgcc acccatgccg
     1081 cccataccgc cagcagcgcc taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg
     1141 cattcggtgg tgatcatcag gccagccaca gaagctgcgt actgcagagc agaacgggtt
     1201 actttggttg ggtccaggat acccatgtcg atcatgttgc cgtattcttc ggttgctgcg
     1261 ttgtaaccgt agttgccgtc gccgccttta acggtgttag caacaacaga cggttcttcg
     1321 ccgcagttca ggacgatctg acgcagcgga gcttccattg cacgcagtgc aactttgata
     1381 cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc tacgcggatc
     1441 agcgcaacac caccaccagc aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg
     1501 gcatcttcaa cgcgtgcttt tttctctttc atttcaactt cggtagcagc acctactttg
     1561 ataactgcaa cgccgcctgc cagtttcgct acgcgctcct gcagtttttc acggtcgtag
     1621 tcagaagttg cttcttcaat ctgctgacgg atctgagcaa cacggccctg gattgcagct
     1681 tcttcaccca cgccatcgat gatagtggtg gtgtctttgt tgatcacaac acgtttagcc
     1741 tgacccaggt cttccagggt tgctttttcc agctccatac cgatctcttc agagattacg
     1801 gtaccgccag tcagggttgc gatatcctgc agcatagctt tacgacgatc gccgaagccc
     1861 ggagctttaa ccgcagcaac tttcacgatg ccacgcatgg tgttaacaac cagagttgct
     1921 agcgcttcgc cttcaacatc ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca
     1981 gcttccagaa ccggcagcat ttcgcggatg ttggagattt tcttgtcagc cagcaggatg
     2041 aacgggcttt ccagttctac tgcgccagtt tccggcttgt tgatgaagta aggagacagg
     2101 tagccacggt cgaactgcat accttcaacc acgtccagtt cgtcctgcag accggtaccg
     2161 tcttcaacgg tgataacgcc ttctttaccg actttgtcca tcgcttcagc gatcagttta
     2221 cctacggttt cgtcggagtt agcggagatg gtaccaacct gagcaatcgc tttagaatca
     2281 gagcacggta cggacagcgc tttcagttct tcaactgcag cggtaaccgc tttgtcgata
     2341 ccacgtttca ggtccatcgg gttcatgccc gcagcaacag ctttcagacc ttcagtgatg
     2401 atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg
     2461 gaggcaactt ctttcaccat ctgcgcaccc atattttcga acttgtcttc cagttcgatt
     2521 tcacgagcaa cggaaacacc atctttggtg atggtcggtg caccgaaaga tttatccaga
     2581 actacgttac ggccttttgg accgagggta actttcactg catctgccag tacgtttacg
     2641 ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta cgtctttagc tgccattatc
     2701 tttattcctt aaattcgtat gttcagtgtc gtgcgcggat tacgcttcaa caattgccag
     2761 aatgtcgctt tcggacatga tcaacacttc ttcattgtcg atcttctcgg atttcacacc
     2821 gtagccatcg ttgaaaataa cgatgtcgcc aactttcacg tccagcggct tcacttcgcc
     2881 attttcaagg atacggccat tgccgacagc cagcacttcg ccgcgggtgg atttagccgc
     2941 tgcagagccg gtcagaacga tgccgccagc agatttagtt tcaacttctt tacgcttgac
     3001 gatcacgcga tcatgcaatg gacgaatatt cattgataac tctcctttga gaaagtccgt
     3061 atctgttatg ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca gagaaatggg
     3121 gatgaggctt cgccccttca agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg
     3181 ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc ttcatcgccg tcataagcga
     3241 atctgattgt gctaccatcg aaaatctacg cagttgccca aaatttgggc gcaatcggat
     3301 atcaacccat gagtggactc aaacaagaac tggggctggc ccagggcatt ggcctgctat
     3361 cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag
     3421 gcaataacag cctgtgggcg tggcccgttt tgattatctt agtgttcccg attgcgattg
     3481 tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta
     3541 tggcgtttgg ttcgcggctt gagcgagtca ccggctggct gtttttatcg gtcattcccg
     3601 tgggtttgcc tgccgcacta caaattgccg ccggattcgg ccaagcaatg tttggctggc
     3661 atagctggca actgttgttg gcagaactcg gtacgctggc gttggtgtgg tatatcggta
     3721 ctctcggtgc cagttccagt gctaatctac aaaccgttat tgccggactt atcgtcgcac
     3781 tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc
     3841 caggaaatat cgaacttacc gggttatttg ctgcgttatc agtgatgttc tggtgttttg
     3901 tcggtctgga agcatttgcc catcttgcct cggaatttaa aaatccagag cgtgattttc
     3961 ctcgtgcttt gatgattggt ctgctgctgg caggattagt ctactggggc tgtacggtag
     4021 tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa
     4081 ttgtagtaca gttgttcggt gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg
     4141 cctgctttgc cagtcttaac atttatatac agagcttcgc ccgcctggtc tggtcgcagg
     4201 cgcaacataa tcctgaccac tacctggcac gcctctcttc tcgccatatc ccgaataatg
     4261 ccctcaatgc ggtgcttggc tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga
     4321 tcaatctgga cgctcttatt atttatgcca atggcatctt tattatgatt tatctgttat
     4381 gcatgctggc aggctgtaaa ttattgcaag gtcgttatcg actactggcg gtggttggcg
     4441 ggctgttatg cgttctgtta ctggcaatgg tcggctggaa aagtctctat gcgctgatca
     4501 tgctggtggg gttatggctg tttctgccaa aacgaaaaac gccggaaaat ggcataacca
     4561 cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg
     4621 tcatcatcct ttcgctgata ctcaccatca aaagtattac cgccaccggt cccggcgcta
     4681 aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc
     4741 ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat
     4801 aaaagcagca aaccggcaat aatcagcgaa acacttttaa tcatctccgc cgcagggttt
     4861 tcacccgccg ccattttttg ctgcatcagc acgaaattct taaagccctg gttacgtacc
     4921 agtgacatac cgataactga cgtgaatata accagcacga gggtcagcaa tacccccaat
     4981 acatgggcaa cctgaataaa gattgaaatc tcaatataga cataaaggaa aatggcaata
     5041 aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc ggctacctga
     5101 atgggttgcg aatcgccttc agcttatatt gtggtcatta gcaaaatttc aagatgtttg
     5161 cgcaactatt tttggtagta atcccaaagc ggtgatctat ttcacaaatt aataattaag
     5221 gggtaaaaac cgacacttaa agtgatccag attacggtag aaatcctcaa gcagcatatg
     5281 atctcgggta ttcggtcgat gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca
     5341 tatattctgt gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa
     5401 acaacattcg tatcgaagaa gatctgttgg gtaccaggga agttccagct gatgcctact
     5461 atggtgttca cactctgaga gcgattgaaa acttctatat cagcaacaac aaaatcagtg
     5521 atattcctga atttgttcgc ggtatggtaa tggttaaaaa agccgcagct atggcaaaca
     5581 aagagctgca aaccattcct aaaagtgtag cgaatgccat cattgccgca tgtgatgaag
     5641 tcctgaacaa cggaaaatgc atggatcagt tcccggtaga cgtctaccag ggcggcgcag
     5701 gtacttccgt aaacatgaac accaacgaag tgctggccaa tatcggtctg gaactgatgg
     5761 gtcaccagaa aggtgaatat cagtacctga acccgaacga ccatgttaac aaatgtcagt
     5821 ccactaacga cgcctacccg accggtttcc gtatcgcagt ttactcttcc ctgattaagc
     5881 tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc gaattccagg
     5941 acatcctgaa aatgggtcgt acccagctgc aggacgcagt accgatgacc ctcggtcagg
     6001 aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg
     6061 aactgctgct ggaagttaac cttggcgcaa cagcaatcgg tactggtctg aacacgccga
     6121 aagagtactc tccgctggca gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac
     6181 cggctgaaga cctgatcgaa gcgacctctg actgcggcgc ttatgttatg gttcacggcg
     6241 cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt
     6301 ctggtccacg tgccggcctg aacgagatca acctgccgga actgcaggcg ggctcttcca
     6361 tcatgccagc taaagtaaac ccggttgttc cggaagtggt taaccaggta tgcttcaaag
     6421 tcatcggtaa cgacaccact gttaccatgg cagcagaagc aggtcagctg cagttgaacg
     6481 ttatggaacc ggtcattggc caggccatgt tcgaatccgt tcacattctg accaacgctt
     6541 gctacaacct gctggaaaaa tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag
     6601 gttacgttta caactctatc ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca
     6661 acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc
     6721 tggaacgcgg tctgttgact gaagcggaac ttgacgatat tttctccgta cagaatctga
     6781 tgcacccggc ttacaaagca aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg
     6841 tagtacaaat aaaaaaggca cgtcagatga cgtgcctttt ttcttgtgag cagtaactta
     6901 aaaataacaa tctaatatca acttgttaaa aaacaaggaa ggctaatatg ctagttgtag
     6961 agctcatcat agttttgctg gcgatcttct tgggcgccag attgggggga ataggtattg
     7021 gttttgcagg cggattgggg gtgctggttc ttgccgctat tggcgttaaa cccggtaaca
     7081 tcccgttcga tgtcatttcc attatcatgg cggttatcgc cgctatttct gccatgcagg
     7141 ttgctggcgg tctggactat ctggttcatc agacagaaaa gctgctgcgc cgtaacccga
     7201 aatacatcac gatcctcgca ccgatcgtga cctatttcct gactatcttt gctggtactg
     7261 gcaacatctc tctggcgaca ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta
     7321 aaccttgccg tccgctctct actgcagtgg tatccgcgca gattgcgatc accgcatcgc
     7381 caatctcagc ggcagtggtt tacatgtctt ccgtgatgga aggtcatggc atcagctacc
     7441 tccatctgct ctccgtggtc atcccgtcca ccctgctggc ggttctggtg atgtccttcc
     7501 tggtcactat gctgttcaac tccaaactct ctgacgatcc gatttatcgc aagcgtctgg
     7561 aagagggctt ggttgaactg cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa
     7621 cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt tatctatgca atcatcaaca
     7681 gcccaagcat gggtctggtt gaaaaaccgc tgatgaacac caccaacgca atcctgatca
     7741 tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc
     7801 tcaactccag caccttcaaa gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt
     7861 ggctgggcga tactttcgtt tccaacaaca tcgactggat caaagatacc gctggtgaag
     7921 tgattcaggg tcatccgtgg ctgctggccg tcatcttctt ctttgcttct gctctgctgt
     7981 actctcaggc tgcaaccgca aaagcactga tgccgatggc tctggcactg aacgtttcac
     8041 cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct gttcattctg ccgacctacc
     8101 cgacgctggt tgctgcggta cagatggatg acacgggtac tacccgtatc ggtaaattcg
     8161 tcttcaacca tccgttcttc atcccgggta ctctgggtgt tgccctggcc gtttgcttcg
     8221 gcttcgtgct gggtagcttc atgctgtaat gatcaatcgc ggggcgttca cgccccgctt
     8281 tctttcccgc cgactaacat ccttcccccg tccgttgtat agtgacctct ctcttgcggt
     8341 tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa gttcgaatac cacgtctgtc
     8401 gtggtgctat gtaccgcacc ggatgaagcg acagcccagg atttagccgc caaagtgctg
     8461 gcggaaaaac tggcggcctg cgcgaccttg atccccggcg ctacctctct ctattactgg
     8521 gaaggtaagc tggagcaaga atacgaagtg cagatgattt taaaaactac cgtatctcac
     8581 cagcaggcac tgctggaatg cctgaagtct catcatccat atcaaacccc ggaacttctg
     8641 gttttacctg ttacacacgg agacacagat tacctctcat ggctcaacgc atctttacgc
     8701 tgattctgct actttgcagc acttccgttt ttgccggatt attcgacgcg ccgggacgtt
     8761 cacaatttgt ccccgcggat caagcctttg cttttgattt tcagcaaaac caacatgacc
     8821 ttaatctgac ctggcagatc aaagacggtt actacctcta ccgtaaacag atccgcatta
     8881 cgccggaaca cgcgaaaatt gccgacgagc agctgccgca aggcgtctgg catgaagatg
     8941 agttttacgg caaaagcgag atttaccgcg atcggctgac gcttcccgta accatcaacc
     9001 aggcgagtgc gggagcgacg ttaactgtca cctaccaggg ctgtgctgat gccggtttct
     9061 gttatccgcc agaaaccaaa accgttccgt taagcgaagt ggtcgccaac aacgcagcgt
     9121 cacagcctgt gtctgtttcg cagcaagagc agcacaccgc gcaattgccc ttttccgcgc
     9181 tctgggcgtt gttgatcggt attggtatcg cctttacgcc atgcgtgctg ccaatgtacc
     9241 cactgatttc tggcatcgtg ctgggtggta aacagcggct ctccactgcc agagcattgt
     9301 tgctgacctt tatttatgtg caggggatgg cactgactta cacggcgctg ggtctggtag
     9361 ttgccgccgc agggttacag ttccaggcgg cgctacagca cccatacgtg ctcattggcc
     9421 tcgccatcgt ctttaccttg ctggcgatgt caatgtttgg cttgtttact ctacaactcc
     9481 cctcttcgct gcaaacacgt ctcacgttga tgagcaatcg ccaacagggc ggctcaccta
     9541 gcggtgtgtt tgttatgggg gcgattgccg gactgatctg ttcaccatgc accaccgcac
     9601 cgcttagcgc gattctgctg tatatcgccc aaagcgggaa catgtggccg ggcggcggca
     9661 cgctttatct ctatgcgttg ggcatgggcc tgccgctgat gctaattacc gtctttggta
     9721 accgcttgct gccgaaaagc ggcccgtgga tggaacaagt caaaaccgcg tttggttttg
     9781 tgatcctcgc actgccggtc ttcctgctgg agcgagtgat tggtgatgta tggggattac
     9841 gcttgtggtc ggcgctgggt gtcgcattct ttggctgggc ctttatcacc agcctacagg
     9901 ctaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct ggcagcggca ttggttagcg
     9961 tgcgcccact tcaggattgg gcatttggtg cgacgcatac cgcgcaaact caaacgcatc
    10021 tcaactttac acaaatcaaa acggtagatg agttaaatca ggcgctcgtt gaagccaaag
    10081 gcaaaccggt gatgttagat ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga
    10141 aatacacctt cagcgacccg caggtgcaaa aagcgttagc agacacggtc ttacttcagg
    10201 ccaacgtcac ggccaacgac gcacaagatg tggcgctgtt aaagcatctt aatgtccttg
    10261 gcctaccgac aattctcttt tttgacggac aaggccagga gcatccacaa gcacgcgtca
    10321 cgggctttat ggatgctgaa accttcagcg cacatttgcg cgatcgccaa ccgtgaacga
    10381 cacttgcagt gggaaagacg gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag
    10441 ccctgaaatt attagaatta caagggattg ccaacaccac gctggagatg gttgctgaac
    10501 gtgtggatta tccactggac gagctacgcc gcttctggcc agacaaagag gcaatcctct
    10561 acgatgcgct gcgttatctt agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg
    10621 acgaaacgca aaccgccgaa caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg
    10681 ttaaaaacaa ccgctatccg ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc
    10741 ctggccaccc tattcatcaa ctggccgatc agcaaaaaag cgctgcctac gatttcaccc
    10801 acgaactgtt aaccacgctg gaagttgacg atccggcgat ggtagcaaag cagatggaac
    10861 tggtgctgga aggctgttta agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca
    10921 ctgcacatcg gctggcggaa gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat
    10981 tgacctgaca gaaacacaga aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct
    11041 tttactgaaa ttaggttgac gagatgtgca gatta
//
LOCUS       DAJTRM010000117        10973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRM010000117 DAJTRM010000000
VERSION     DAJTRM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.117"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            399..1229
                     /locus_tag="OO322_003460"
     CDS             399..1229
                     /locus_tag="OO322_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5908209.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1370..2143)
                     /gene="uxuR"
                     /locus_tag="OO322_003461"
     CDS             complement(1370..2143)
                     /gene="uxuR"
                     /locus_tag="OO322_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5908210.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2358..3818)
                     /locus_tag="OO322_003462"
     CDS             complement(2358..3818)
                     /locus_tag="OO322_003462"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5908211.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3899..5083)
                     /gene="uxuA"
                     /locus_tag="OO322_003463"
     CDS             complement(3899..5083)
                     /gene="uxuA"
                     /locus_tag="OO322_003463"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5908212.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5423..6766
                     /gene="gntP"
                     /locus_tag="OO322_003464"
     CDS             5423..6766
                     /gene="gntP"
                     /locus_tag="OO322_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5908213.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7017..7919)
                     /gene="fimH"
                     /locus_tag="OO322_003465"
     CDS             complement(7017..7919)
                     /gene="fimH"
                     /locus_tag="OO322_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5908214.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7939..8442)
                     /gene="fimG"
                     /locus_tag="OO322_003466"
     CDS             complement(7939..8442)
                     /gene="fimG"
                     /locus_tag="OO322_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5908215.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8455..8985)
                     /gene="fimF"
                     /locus_tag="OO322_003467"
     CDS             complement(8455..8985)
                     /gene="fimF"
                     /locus_tag="OO322_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5908216.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8995..>10973)
                     /gene="fimD"
                     /locus_tag="OO322_003468"
     CDS             complement(8995..>10973)
                     /gene="fimD"
                     /locus_tag="OO322_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5908217.1"
                     /translation="RLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLG
                     DGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYGIYN
                     STVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITA
                     GEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALS
                     VDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSR
                     MNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWG
                     TSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKS
                     QWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYAT
                     LNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKD
                     AKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIV
                     RAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQ
                     VKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2662 a         2808 c         2671 g         2832 t
ORIGIN      
        1 aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc cgcataatca
       61 gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac atatcaaata
      121 catcaaaaat accattcaca ggcattatta atatagacct caatatagca atatcaaatt
      181 ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat gagcgttttt
      241 tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat atcactacaa
      301 cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt tatctatttt
      361 tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca ttaagcaatg
      421 ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa gtcgacagtg
      481 agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat cttctgcctc
      541 ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt agaaaactta
      601 atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga gaattcaatg
      661 gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca gctttagacg
      721 aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca gaaggtttac
      781 ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca gttcatgaaa
      841 taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata ggatatattt
      901 caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga attgatatct
      961 atattgatca gataataact gtctcagggg tagcaaattc ttccggattc gcccttgccg
     1021 cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata gaagcctatc
     1081 ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt gatgaagatg
     1141 cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt gaattaaaaa
     1201 atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact aatattcagg
     1261 ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt gcgacttctt
     1321 aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt caggcgtcga
     1381 ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg tcaacgttcg
     1441 agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc agcttcgccg
     1501 ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc aaccactctt
     1561 tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga ttgttttccc
     1621 gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt gcttcagcaa
     1681 tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg gaagccagtt
     1741 cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg gtagcctgca
     1801 gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac agctcaaaag
     1861 gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg cctgagctat
     1921 caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc tccagcatga
     1981 tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt tcacgctccg
     2041 gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg atcatcgccc
     2101 cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg tgtccgttag
     2161 gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc ctacaacggg
     2221 caattttacg gcccccgtta atctcaaact ataccattag cactggaagt tggcacgtac
     2281 aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac cagtaagtct
     2341 gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc
     2401 tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag atcatcggca
     2461 aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata ctgcgcgtta
     2521 atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc ctgctcatcc
     2581 acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg ccagctgccg
     2641 ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt ctggctgccg
     2701 tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg ctcgatcagc
     2761 agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg cgcttgttcc
     2821 tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt gtcggcaatg
     2881 gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc gttcagcata
     2941 cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc cactttatcc
     3001 cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa cggttcgcag
     3061 gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa ggtttctggc
     3121 gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt ctcttcaatc
     3181 catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac cttcgccaca
     3241 tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag ccctttttca
     3301 cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt
     3361 tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc cgcatcagcg
     3421 cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg cgccatcgcg
     3481 ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat cgaaccgata
     3541 attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag ttgctgtttt
     3601 ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc gcagatgccc
     3661 cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg cgcgcggtga
     3721 aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc ccatgacggg
     3781 cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag aatccgtctt
     3841 cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc ggtgggtatt
     3901 aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg gccaggcctt
     3961 tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg tccagcatct
     4021 gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct ttacgacggt
     4081 gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg ttcaggtgcg
     4141 ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga tgggtgaagt
     4201 aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca cgcacgccgt
     4261 aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca accatccact
     4321 gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc ggcggatcgt
     4381 ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc gctttcagga
     4441 agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc tccaggtgct
     4501 tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca atgatgttac
     4561 gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct gcctgagcaa
     4621 tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc atttcgaatg
     4681 cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc acgtattcga
     4741 ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg cgaatgccac
     4801 actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca tagttgccag
     4861 tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac accaggcctg
     4921 cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact tcgccgttcg
     4981 ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca tcagctaaag
     5041 aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt cctctttcac
     5101 atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta tccggatgaa
     5161 caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt ctaccttttg
     5221 gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa tttcactctc
     5281 atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca gaggatgtga
     5341 cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat aacaacggtt
     5401 caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg tattcggcat
     5461 tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg ctttgttggt
     5521 ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc tgcacaccat
     5581 gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt tcggtgcggt
     5641 catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata cgctgctggc
     5701 gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta ttttcggtct
     5761 ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg ttattgccgc
     5821 cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg ccactaccgc
     5881 acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga
     5941 tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa tctgcgcagg
     6001 tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat tcctgaaagc
     6061 agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc tggtgccgct
     6121 gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg taaaagatac
     6181 ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt ttattgcgat
     6241 ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt gggtgatgaa
     6301 cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg gtgcgggtgg
     6361 cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca tgttgatgtc
     6421 ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa ttcgtctggc
     6481 gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg ctgcaatcct
     6541 tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac tggcgacggc
     6601 tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt tcaaaggtta
     6661 ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc tggtcaactc
     6721 cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga acaccagagc
     6781 ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg acagtcccct
     6841 cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc gctttgttta
     6901 tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt gcaggttttg
     6961 taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta atttttttat
     7021 tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac ctgccctccg
     7081 gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac tgctcctaac
     7141 gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc
     7201 tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg
     7261 ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc
     7321 actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcacaaccg
     7381 ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg aaaatcatcg
     7441 ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc agctttaata
     7501 gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc
     7561 gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg
     7621 ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc cgagcctcgt
     7681 tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc
     7741 gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt tacataaaca
     7801 ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca ggcgaatgac
     7861 caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc tcgtttcatt
     7921 acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt
     7981 tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga
     8041 ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg tgttgccact
     8101 gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt
     8161 gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt
     8221 caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa gactgaaaga
     8281 ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg gtttggcgac
     8341 gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc
     8401 caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct ccagttactg
     8461 atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag gcacctgtgt
     8521 cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca gggtcgtcca
     8581 cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat tcagaagctg
     8641 tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg
     8701 gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac cacagggtga
     8761 cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc
     8821 cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt
     8881 atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc gacttaccgc
     8941 cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg
     9001 acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt
     9061 ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg
     9121 cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct ggctactctc
     9181 tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg tcatgagcag
     9241 ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag tgggaacaac
     9301 gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat ccagcgccac
     9361 tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc agtcggtacg
     9421 cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagcaccac
     9481 cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata ccccaccgct
     9541 gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat tggcattgcc
     9601 gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac catcgcctcc
     9661 cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca acgtaccgta
     9721 tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt agctggcact
     9781 ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac
     9841 gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca ggctatagct
     9901 gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt gctcatcgac
     9961 attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc
    10021 gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga ggttgtaata
    10081 gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt tgtagccatt
    10141 cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc
    10201 cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa aacgcaccga
    10261 ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga
    10321 cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac gataacgatc
    10381 cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact
    10441 ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc ctgccgtaat
    10501 ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa
    10561 aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac tattacctgc
    10621 ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat aaatgccata
    10681 tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga tcaccggggc
    10741 aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa
    10801 gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca gccgggaacg
    10861 taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat ttttgctacc
    10921 tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac gcc
//
LOCUS       DAJTRM010000118        10959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRM010000118 DAJTRM010000000
VERSION     DAJTRM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.118"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..460)
                     /gene="intS"
                     /locus_tag="OO322_003469"
     CDS             complement(<1..460)
                     /gene="intS"
                     /locus_tag="OO322_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5908218.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(622..696)
                     /locus_tag="OO322_003470"
     tRNA            complement(622..696)
                     /locus_tag="OO322_003470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="OO322_003471"
                     /pseudo
     CDS             complement(770..1702)
                     /locus_tag="OO322_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="OO322_003472"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="OO322_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5908219.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2933..3991)
                     /locus_tag="OO322_003473"
     CDS             complement(2933..3991)
                     /locus_tag="OO322_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908220.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="OO322_003474"
     CDS             complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="OO322_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5908221.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6069..6353
                     /locus_tag="OO322_003475"
     CDS             6069..6353
                     /locus_tag="OO322_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5908222.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6533..7843
                     /gene="fadI"
                     /locus_tag="OO322_003476"
     CDS             6533..7843
                     /gene="fadI"
                     /locus_tag="OO322_003476"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5908223.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7843..9987
                     /gene="fadJ"
                     /locus_tag="OO322_003477"
     CDS             7843..9987
                     /gene="fadJ"
                     /locus_tag="OO322_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5908224.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10190..10675
                     /gene="sixA"
                     /locus_tag="OO322_003478"
     CDS             10190..10675
                     /gene="sixA"
                     /locus_tag="OO322_003478"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5908225.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2738 a         2526 c         2726 g         2969 t
ORIGIN      
        1 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
       61 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      121 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      181 taaatgaatt gttgttagac gaagactttt tcgcctttac cggatcgatg ccagccgaga
      241 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      301 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      361 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      421 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      481 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      541 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      601 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      661 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggtgg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcggca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgctcggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctaaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatgctc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gtttgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat
     2941 catcaaaatt aacacctagt gcagatagtt gattaatttc attttgatat ttcgtctggc
     3001 gattttgaag tcttttttca actttatctt cgagattctt aaagtctttg tatttatttt
     3061 caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct tttacattat
     3121 cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt
     3181 taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat
     3241 cggaaccagt tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag
     3301 cactcctggc gttttcacta caatattggg tgagactatc accattaata cgaattcctc
     3361 tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt
     3421 tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac caggtaaata
     3481 agtcagttgg ttcgcttttc ccgctctcat caaattccgt taccgtatgt ctgagtttgt
     3541 gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt aatgcgctga
     3601 agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca ttaatgatag
     3661 tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct tggttgtcga
     3721 taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta actgaggaat
     3781 tattaatatt agatctataa ggatcgtcgt ttactcgtat ggcgtttttt ataagctcaa
     3841 gtagatgttt aatacaggtg cttattcctt ccttaccatt aaaaaggttt ttgaactctt
     3901 gtactaatcc attaatttca tcattgttat tttcattgat ggattctggg taagctgcgc
     3961 tggtattgtt gattgaaata gatggtagca tgattttatt ccatgttgat tgatttgtta
     4021 caaatatata ttacctaatg aaatagacta tcaaaaaacg ctcatttatt tagaacctat
     4081 ctttaatttg agtgtataag cgcacaatag ccaggggggt tgagtatcct ggaaatgcag
     4141 aaaagtaaac tctttgagaa ggacgagcag aacgcttgaa aatatgcatt tcccttcagg
     4201 tttgtctgtg gataccactt actgatttga aagggcaagc tgccatcagg ctgtttgcaa
     4261 aactcaggag gcaagcaatg aggaaactac tttcggtgaa gtgggggagt taaagtcacc
     4321 tgcatagcag gtgactttat ccaggcgaac gcgttatcag aacgcgtagt taaagttagt
     4381 accgaacagc caggctttac cttcagactc gaactggtat gggccttcgt taattttcac
     4441 gctctgaccg tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta
     4501 agtcgtacct gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg
     4561 agccggaact gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta
     4621 gtaataagtg gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa
     4681 cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta
     4741 agccaggcta tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca
     4801 catttcaggc aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg
     4861 aattggcaaa ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa
     4921 gtcaattttc acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata
     4981 caggattccg gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt
     5041 attactgtcg ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc
     5101 aggagattgc ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat
     5161 tttcgcgcga gcgtagatgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg
     5221 atacgcaccg cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc
     5281 gcctgcataa gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc
     5341 ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg
     5401 cgcgatgtta tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc
     5461 cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc
     5521 ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg
     5581 gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga
     5641 gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac
     5701 cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa
     5761 gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca
     5821 gctagaatgt tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc
     5881 agctggcaaa atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat
     5941 ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact
     6001 ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga
     6061 aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg
     6121 gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaaaccgcg
     6181 cagaagcaga acaaacgctg gcggcgctga ctgaaaaatc ccgtagcgtg aaatccgaac
     6241 cgtgcaaaat ctccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt
     6301 tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct
     6361 cgggtacgta cgcccctgtc agttgctggc aggggcgttt tcttttcttt cttctgtctt
     6421 cactgtgtca tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatggtt
     6481 aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca
     6541 ggttttacca ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac
     6601 gccttttgcc cgccaggcga cggcttttca tggcattccc acggttgatt tagggaagat
     6661 ggtggtaggc gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt
     6721 ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg
     6781 taccggaatg aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt
     6841 ccaagcggtt gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc
     6901 cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgtacgt
     6961 gctggttgat gtcaacaaag ctcgaaccat gagccagcgc ctgaaactct tctctcgcct
     7021 gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg
     7081 gatgggcgac accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga
     7141 tgcattagcg caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa
     7201 agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa
     7261 tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa
     7321 acacggaacg gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat
     7381 cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag
     7441 ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccag cctggtcaac
     7501 accgctggcg ctggaacgtg ccggtttgac gatgagcgaa ctgacattga tcgatatgca
     7561 cgaagccttt gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc
     7621 tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt
     7681 gcttggcggt tcgattgctt acggacatcc cttcgcggcg accggcgcgc ggatgattac
     7741 ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc
     7801 tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat ggcatcagcg
     7861 tttactctta atgttcgtct ggacaacatt gctatcatca ccatcgacgt accggatgag
     7921 aaaatgaata ccctgaaggc ggaatttgcc tcgcaggtgc gcgccattat taagcaactc
     7981 cgtgaaaaca aagagttgcg cggcgtggtg tttatctccg cgaaaccgga caattttatc
     8041 gccggagcag acatcaacat gattggcaac tgcaaaacgg cgcaagaagc ggaagcactg
     8101 gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg
     8161 gctattcatg gcgcttgcct gggcggtggg ctggagctgg cgctggcgtg ccacggtcgc
     8221 gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta
     8281 cccggttcag gcggcaccca gcgtttaccg cgtctgatag gcgtcagtac agcattagag
     8341 atgattctca ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat
     8401 gacgttgttc cgcactccat tttgctggaa gccgctgttg agctggcgaa gaaggatcgc
     8461 ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg
     8521 ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg
     8581 acagaacgca tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggctat
     8641 gacgctgaag ctcgcgcctt tggcgaactg gcgatgacgc cgcaatcgca agctttgcgt
     8701 agtatctttt ttgccagtac ggacgtgaag aaagatcccg gtagtgatgc accgcctgcg
     8761 ccattaaaca gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc
     8821 actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat
     8881 catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa
     8941 gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt
     9001 gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg
     9061 gtggcggaag tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa cacgtcatct
     9121 ttaccgattg gtgatatcgc cgctcacgcc gcgcgacctg agcaagttat tggcctgcat
     9181 ttcttcagtc cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg
     9241 gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc
     9301 gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct
     9361 atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt
     9421 ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa
     9481 attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt
     9541 tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat
     9601 ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg ccatttaccc gctcattggc
     9661 gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg
     9721 ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat
     9781 attggtgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc
     9841 gattctctcg gcgcgggcga agtggttgca ataatgcaac gactggccac gcagtatggt
     9901 tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg
     9961 aaaacaactg caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg
    10021 gcggagtgtt ggtcaaaatg taaacgcata ttgactatac ttacgccatt gaggtaaaaa
    10081 acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg
    10141 tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt
    10201 tatcatgcgt cacggcgacg cagccctcga ttccgccagt gattcggttc gtcctctgac
    10261 cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga
    10321 aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg
    10381 ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga
    10441 tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact
    10501 ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac
    10561 gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg
    10621 tacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgtgt
    10681 tccaaattat gcccaatgag gtagattcat tgggcataat tgctcattag gcagcggata
    10741 tctgtcaacc tcactactat cgaagactct ccttatctat aaataaaaca ctttctacat
    10801 tcattcggaa attattcttc tacgcgtatc ttgattttgc ccaaagttac atgttgaaat
    10861 gaataatatc tgtttgacac tcattgtcat ttattaatac agataataga gaaaatagag
    10921 ttgaataatc aggcaggatg taattgaaac ttgattaaa
//
LOCUS       DAJTRM010000119        10936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRM010000119 DAJTRM010000000
VERSION     DAJTRM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.119"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO322_003479"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO322_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5908226.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO322_003480"
     CDS             complement(1433..1759)
                     /locus_tag="OO322_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5908227.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO322_003481"
     CDS             1894..2235
                     /locus_tag="OO322_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5908228.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO322_003482"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO322_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO322_003483"
     CDS             complement(2833..3543)
                     /locus_tag="OO322_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5908229.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO322_003484"
     CDS             3651..3956
                     /locus_tag="OO322_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5908230.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO322_003485"
     CDS             4155..4475
                     /locus_tag="OO322_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5908231.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO322_003486"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO322_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO322_003487"
     CDS             complement(6299..7288)
                     /locus_tag="OO322_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908232.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO322_003488"
     CDS             complement(7373..7864)
                     /locus_tag="OO322_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908233.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO322_003489"
     CDS             complement(8185..8685)
                     /locus_tag="OO322_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908234.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO322_003490"
     CDS             complement(8675..9190)
                     /locus_tag="OO322_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908235.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO322_003491"
     CDS             complement(9621..9989)
                     /locus_tag="OO322_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908236.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..>10936)
                     /locus_tag="OO322_003492"
     CDS             complement(10401..>10936)
                     /locus_tag="OO322_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5908237.1"
                     /translation="RHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLDNGLV
                     CIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTPSAYS
                     SWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDLYG
                     KGHAVGRLADVLKTALL"
BASE COUNT         2806 a         2781 c         2703 g         2646 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca
    10861 gaccgagcgc cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg
    10921 tgagccgtgt gtcgtg
//
LOCUS       DAJTRM010000120        10926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRM010000120 DAJTRM010000000
VERSION     DAJTRM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.120"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            154..504
                     /locus_tag="OO322_003493"
     CDS             154..504
                     /locus_tag="OO322_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5908238.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO322_003494"
     CDS             557..952
                     /locus_tag="OO322_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5908239.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO322_003495"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO322_003495"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5908240.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO322_003496"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO322_003496"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5908241.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO322_003497"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO322_003497"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5908242.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO322_003498"
     CDS             complement(5323..6423)
                     /locus_tag="OO322_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5908243.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO322_003499"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO322_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5908244.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO322_003500"
     CDS             complement(7571..8137)
                     /locus_tag="OO322_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5908245.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO322_003501"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO322_003501"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5908246.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OO322_003502"
     CDS             10263..10751
                     /locus_tag="OO322_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5908247.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJTRM010000121        10465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRM010000121 DAJTRM010000000
VERSION     DAJTRM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.121"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            4..204
                     /gene="cspG"
                     /locus_tag="OO322_003503"
     CDS             4..204
                     /gene="cspG"
                     /locus_tag="OO322_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5908248.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            215..403
                     /locus_tag="OO322_003504"
     CDS             215..403
                     /locus_tag="OO322_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5908249.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            378..608
                     /gene="ymcE"
                     /locus_tag="OO322_003505"
     CDS             378..608
                     /gene="ymcE"
                     /locus_tag="OO322_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5908250.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            598..771
                     /locus_tag="OO322_003506"
     CDS             598..771
                     /locus_tag="OO322_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5908251.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(820..1893)
                     /locus_tag="OO322_003507"
     CDS             complement(820..1893)
                     /locus_tag="OO322_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5908252.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1995..4706)
                     /gene="torS"
                     /locus_tag="OO322_003508"
                     /pseudo
     CDS             complement(1995..4706)
                     /gene="torS"
                     /locus_tag="OO322_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4789..5817
                     /gene="torT"
                     /locus_tag="OO322_003509"
     CDS             4789..5817
                     /gene="torT"
                     /locus_tag="OO322_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5908253.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5790..6482)
                     /gene="torR"
                     /locus_tag="OO322_003510"
     CDS             complement(5790..6482)
                     /gene="torR"
                     /locus_tag="OO322_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5908254.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6569..7741
                     /gene="torC"
                     /locus_tag="OO322_003511"
     CDS             6569..7741
                     /gene="torC"
                     /locus_tag="OO322_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5908255.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7741..10281
                     /gene="torA"
                     /locus_tag="OO322_003512"
                     /pseudo
     CDS             7741..10281
                     /gene="torA"
                     /locus_tag="OO322_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10278..>10465
                     /gene="torD"
                     /locus_tag="OO322_003513"
     CDS             10278..>10465
                     /gene="torD"
                     /locus_tag="OO322_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5908256.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2685 c         2788 g         2467 t
ORIGIN      
        1 aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct
       61 gatgatggca gcaaagacgt tttcgtccat ttcaccgcca tccagagcaa tgaattccgc
      121 acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg
      181 gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg
      241 tccatgttgt catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc
      301 tttgggcgct aagtgtattt tttgtaaatc gactatgatc acctttgata acgtcgcgct
      361 gcaaatacgc actgaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg
      421 gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc
      481 tgttgattat tgaatctttc gggaagatgg cttataacat ttcttacctg accagggtac
      541 cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagacgag gttaacagtg
      601 aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa
      661 atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt catcgcacga
      721 gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa
      781 catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
      841 ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata ctcccgcata
      901 cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc
      961 agacaacgca aaggcttttt tatttgctgc aggtttttgc agtgaaaagg tcaatgttga
     1021 ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
     1081 aattaattta tctaccggga tccgtgatgg gcactttttt gcgcatttgc cacaatcgat
     1141 acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag
     1201 cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag
     1261 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
     1321 aacgtccatc accacgctat agggcgacaa cataaaatag tgaatcgcct gagcgggcat
     1381 caataacgcg atatagagaa ataaactcaa cagcaaatac ttcacgccgc gcagaggaat
     1441 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
     1501 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
     1561 actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg
     1621 acctgttgca aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat
     1681 acctcccggt ctggcgacaa atgtcgtact acctgccgtt tcgtaatagc gcacccaata
     1741 ccagaacgtg atggcaatat aaatattcat tgccaatagt aagaattgcg tcgctttacg
     1801 ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg
     1861 ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata
     1921 ccaaatgcca ctattctagt tattcttaac tggctgatat tgattcaaat cgcgttcagg
     1981 tctttcttat tcaatcatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtgga
     2041 aggggggcag ataatggctg ctgctccagt tgtgcgcata gctgactggc gctacgcatt
     2101 cccagactcg agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctgtcc
     2161 tggctggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tttaaataat
     2221 gccagccatt cgtggatctt ctccgtcccc attaactgag cgtcttcatt gagttgcgag
     2281 acatccagtg gtagatcgtt attggcttgc agttggagat agtgcgccag taactgaccg
     2341 agcacttcac gcggcaccgg tttagggata atcccgcgaa atagtgaact ggtacgctgg
     2401 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
     2461 tgtgccagtt gccgggcaag ggtaatgccg tcgatatccg gcagatcaaa atccaccagt
     2521 gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaata
     2581 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
     2641 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt ggctgttttt
     2701 ggcacgggtg cagtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
     2761 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
     2821 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
     2881 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
     2941 ccatcagtgc gactacgcag gataatgtgc ccttcgtcag taaaacgcag ggcattgctc
     3001 aataggttgg taatgacctg acgaatacgt cgtggatcgc ccattaacgc gctcggtaca
     3061 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
     3121 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac tgaaacattt
     3181 ttgccacctg cttcgatagc cgaataatcg agaatatcgt tgaggatggt cagcaacgat
     3241 tcgccagagt cggtaatcgc ctgcaaatca tcacgctggg cgttaagtgc ggggttatct
     3301 gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
     3361 gccgtcagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
     3421 tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
     3481 tcgcggtggc gattcagtgc atgaacactg ctgcgaaacg catccatcag ccgcccgatg
     3541 gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
     3601 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgaatgagcg ataaaccacg
     3661 cgccagagga tcataatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat
     3721 tgcccgcgtg cactggcttt ttccagatgt gccagtccat gctgattgtg cagctcaatg
     3781 gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat attattttgg
     3841 gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc caacagatcg
     3901 ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaactcc cggatcttca
     3961 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
     4021 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
     4081 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
     4141 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
     4201 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgctccag gcgtgcgatc
     4261 tcatcggcgg cagctatctg ctgactgagt tgccgttgtt gctggcgtag ttgcagacgc
     4321 cgccccacca gttccccttg ctgacgtaat gaacgggaga tctcctgctc ctgttgttca
     4381 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
     4441 ctttatgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
     4501 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
     4561 atggtaggaa tcaatgcctg agtattgtct ttttccacct ggctgataaa gcgcaggttg
     4621 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
     4681 agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg
     4741 caaagtggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
     4801 ctatttttac ttctttccct tttcatgttg tcggcatttt cggctgataa cctgttgcgc
     4861 tggcatgatg cgcagcattt cacggtgcaa gcctctatgc cgcttaaagc caaacgcgta
     4921 tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat
     4981 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
     5041 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
     5101 gccattttgc tcggtagtag cacgacgtca tttcccgacc tgcaaaagca ggtagcaaat
     5161 ctgccggtga tcgaactggt aaatgctctt gatgatcccc aggtgaaaag ccgcgttggt
     5221 gtaccctggt ttcagatggg ctatcaacct gggcgatatt tggtgcaatg ggcgcacggt
     5281 aaaccactga acgtgctgct gatgcccgga cccgataacg ccgggggcag taaggagatg
     5341 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
     5401 ggtgataacg atattgaaat ccagcgcaac ctgttgcagg agatgctgga acgccaccca
     5461 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg tgaagggcgt
     5521 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
     5581 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
     5641 gcagttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
     5701 ccgattttag ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg ctcgctgtca
     5761 ccggggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct
     5821 tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg acgaattaac
     5881 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgcagcaga
     5941 cgctcacggc tgagaatttc gcccggatta gtcacaaatg ccaccaacat ttcatactct
     6001 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
     6061 caataacccg caaagcgata gcagtagtcc tgagtgtgcg gttgagcttg tcgcgcgagg
     6121 tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg
     6181 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
     6241 ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg
     6301 ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccggctg attctgcata
     6361 atttcccgta gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
     6421 taggattgta atcgcgcctg ggtaaccggc ttatcttcaa caataacaat gtgatgtggc
     6481 atcagagggt tttactcatt ctgttcatat ctgttcatat ttgctcatta agatcgcttc
     6541 actaaaccat aattctacag gggttattat gcggaaactc tggaacgcgc tacgccgacc
     6601 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6661 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6721 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6781 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6841 gcgcaaactg gaagcgagca acgacattta ccagaccttt attgcccact ccattgatac
     6901 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6961 agaaaacaac tcggcaacct gtcgctcctg ccataactac gatgcgatgg atcatgccag
     7021 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     7081 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     7141 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     7201 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     7261 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     7321 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     7381 ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacca ccgttgccga
     7441 caccaatacc gagtggagca agttgcaggc caccgcgtgg atgaagaaag gcgacatggt
     7501 gaacgatatc aaaccgatct gggcctatgc ggattcgttg tacaacggca cctgtaacga
     7561 gtgccacggc gcaccggaaa tcgcccactt tgacgctaac ggttggatcg gcacgctcaa
     7621 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcactttgt tgaaatatct
     7681 gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7741 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7801 ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc aactgcggcg
     7861 caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc gcactggggg
     7921 gctatccgcg cgatggtgaa ggatggtcgc tttgtggcgg caaaaccgtt cgaactggat
     7981 aaatatccgt cgaaaatgat cgccggattg ccggatcacg tacacaacgc ggcacgtatt
     8041 cgttatccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga cacctcccag
     8101 cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat gttctatgaa
     8161 gaactggaac gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc cagtggttgg
     8221 caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat tgccttgcat
     8281 ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt gatcctgccg
     8341 cgcgtagtcg gttcaatgga agtgtatgaa cagcaaacct cctggccgct ggtattgcag
     8401 aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca agcgaactgg
     8461 tggtgcccgg atcacgatgt ttatgaatat tacgagcagc taaaagcgaa agtcgccgcc
     8521 ggtgaagttg aggtcatcag catcgatccg gttgtcacat ccacccatga gtatctgggg
     8581 cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct gcaactggcg
     8641 ctggcacata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc taactactgt
     8701 gtgggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca gccgaaagat
     8761 gccgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg gctggcgcgg
     8821 cagatggcgg cgaacagaac gcaaattatt gctggctggt gcgtgcagcg tatgcagcac
     8881 ggtgaacagt gggcgtggat gattgtggtt ctggcggcga tgctggggca aattggcctg
     8941 ccaggtggtg gttttggctg gcactacaac ggcgcaggca cgccggggcg taaaggcgtt
     9001 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
     9061 aaaggctaca gcagcactat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     9121 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
     9181 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     9241 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
     9301 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
     9361 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgtagttcga ggcgcgcaac
     9421 gactttgata ttttccgcga gctgtgccgt cgctttaatc gtgaagaagc ctttaccgaa
     9481 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
     9541 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
     9601 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
     9661 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
     9721 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
     9781 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
     9841 tcgcagctat gtgagtcgga aacgctgcgt cagcaatata cggtagcggg taaagagcca
     9901 gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
     9961 gtctttaacg ctcgcggtca ggtgttggca ggagcagtag tttctgaccg ctatgcaccc
    10021 ggcgtggcgc gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
    10081 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcgggac ttcgcagctg
    10141 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggaacagtg
    10201 gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga atatgttccc
    10261 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
    10321 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    10381 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctcact gcggcggtga
    10441 atgatctgga aaacagtatt gccac
//
LOCUS       DAJTRM010000122        10279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRM010000122 DAJTRM010000000
VERSION     DAJTRM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.122"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1214
                     /locus_tag="OO322_003514"
     CDS             <1..1214
                     /locus_tag="OO322_003514"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5908257.1"
                     /translation="SYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPY
                     VIDGSELTLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSL
                     ARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITA
                     ETEAPEEAISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDI
                     WLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKL
                     VSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYR
                     FRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDRLHSR
                     KLRTFNEPPFR"
     gene            1557..2273
                     /locus_tag="OO322_003515"
     CDS             1557..2273
                     /locus_tag="OO322_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5908258.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2729..2804)
                     /locus_tag="OO322_003516"
     tRNA            complement(2729..2804)
                     /locus_tag="OO322_003516"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2769..2771),aa:Asn,seq:gtt)
     gene            complement(2905..4532)
                     /gene="yeeO"
                     /locus_tag="OO322_003517"
                     /pseudo
     CDS             complement(2905..4532)
                     /gene="yeeO"
                     /locus_tag="OO322_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4537..4612
                     /locus_tag="OO322_003518"
     tRNA            4537..4612
                     /locus_tag="OO322_003518"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4570..4572,aa:Asn,seq:gtt)
     gene            complement(4650..5600)
                     /gene="cbl"
                     /locus_tag="OO322_003519"
     CDS             complement(4650..5600)
                     /gene="cbl"
                     /locus_tag="OO322_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5908259.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5702..6619)
                     /gene="nac"
                     /locus_tag="OO322_003520"
     CDS             complement(5702..6619)
                     /gene="nac"
                     /locus_tag="OO322_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5908260.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            6947..7022
                     /locus_tag="OO322_003521"
     tRNA            6947..7022
                     /locus_tag="OO322_003521"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6980..6982,aa:Asn,seq:gtt)
     gene            complement(7078..8013)
                     /gene="ldtA"
                     /locus_tag="OO322_003522"
     CDS             complement(7078..8013)
                     /gene="ldtA"
                     /locus_tag="OO322_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5908261.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8075..9154)
                     /gene="cobT"
                     /locus_tag="OO322_003523"
     CDS             complement(8075..9154)
                     /gene="cobT"
                     /locus_tag="OO322_003523"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5908262.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9166..9909)
                     /gene="cobS"
                     /locus_tag="OO322_003524"
     CDS             complement(9166..9909)
                     /gene="cobS"
                     /locus_tag="OO322_003524"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5908263.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(9906..>10279)
                     /locus_tag="OO322_003525"
     CDS             complement(9906..>10279)
                     /locus_tag="OO322_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5908264.1"
                     /translation="ERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2883 a         2620 c         2210 g         2566 t
ORIGIN      
        1 gcagctacac caccacgatt actcgcccta ctctctttcg ttcgtatctg aatgagcaac
       61 taacgttgct ttatcaggat tatggtgcgc atatctctgt gcaaccctcg caacatgaaa
      121 tcccttatcc ttatgtcatc gatggctctg aattgacact tgatcgctca atgagcgctg
      181 atttaactcg ctacttcccg acaacagaac tggcgcaaat cggcgatgaa actgcagacg
      241 gcatttatca tccaacggaa ttctccccgc tatcgcattt tgatgcgcgc cgcgtcgatt
      301 tttccctcgc acggttgcgc cattataccg gtacgccagt agaacatttt caaccgttcg
      361 tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga tgcagccaga
      421 tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac tggatcactg
      481 ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa caccagatgc
      541 cagcatggca tttaattacc gctgatggtc agggtattac tctggtgaat attggcgtgg
      601 gaccgtccaa tgctaaaacc atctgcgatc atctggcagt gctacgcccg gatatctggt
      661 tgatgattgg tcactgtggc ggattacgtg aaagccaggc cattggagat tatgtccttg
      721 cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc gatataccta
      781 ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc taccaagtta gtcagcggca
      841 ggcctggtga ggaagtcaaa cagcggctgc gtacaggtac tgtggtaacc acagatgaca
      901 ggaactggga gttacgttac tcagcttctg cacttcgttt taacttaagc cgggccgtag
      961 caattgatat ggaaagtgca accattgccg cgcaaggata tcgtttccgc gtgccatacg
     1021 ggacactact gtgtgtttca gataaaccgt tacatggtga gattaagctt cccggccagg
     1081 ctaaccgttt ttatgaaggc gctatttccg aacacctgca aattggcatt cgggcgatcg
     1141 atttgctgcg cgcagaaggc gaccgactgc attcacgtaa attacgaacc tttaatgagc
     1201 cgccgttccg ataataagtt atgaaccttc ttatcagaaa tagatgtgaa ggattaagta
     1261 agacatctgg caaatagcct gcaataaacg cgggctgctt gcttccttgg gcagatgcga
     1321 gttttattat cgtcttaatg atttccacat attaaaagca agtatgcttt caaaacacaa
     1381 ttataaaaaa tcccgccaac aacattagtt tttataaaat taaatataag attatggctt
     1441 tagaatattt ttatttctaa tagacgagat ttttcctgtt atgatataat atgccgaatt
     1501 aacacatgtt aacgatttac cagtaatgta aataaatttt cgaggagatc attccagtgg
     1561 gacgtaaatg ggccaatatt gttgctaaaa aaacggctaa agacggtgca acgtctaaaa
     1621 tttatgcaaa attcggtgta gaaatctatg ctgctgccaa acaaggtgaa cccgatccag
     1681 aattaaacac atctttaaaa ttcgttattg aacgtgcaaa gcaggcacaa gttccaaagc
     1741 acgttattga taaagcaatt gataaagcca aaggcggcgg agatgaaacg ttcgtacagg
     1801 gacgttatga aggctttggt cctaatggct caatgattat tgctgagaca ttgacttcaa
     1861 atgttaaccg tacgattgct aacgttcgca caattttcaa taaaaaaggc ggcaatatcg
     1921 gagcggcagg ttctgtcagc tatatgtttg acaatacggg tgtgattgta tttaaaggaa
     1981 cagaccctga ccatattttt gaaattttgc ttgaagctga agttgatgtt cgtgatgtaa
     2041 ctgaagaaga aggtaatatt gttatttata ctgaacctgc tgaccttcat aaaggaatcg
     2101 cggcgctaaa agcagctgga atcactgagt tctcaacaac agaattagaa atgattgctc
     2161 aatctgaagt tgagctttcc ccagaagatt tagaaatctt tgaagggctt gttgatgccc
     2221 ttgaagatga cgacgatgta caaaaagttt atcataacgt cgcaaatctc taattatatt
     2281 ttaaagaaat ctgtctttat ggcagatttc tttaatctca tataattctt ataaaaaata
     2341 taatatttaa ctcgtcatat tgcttataaa aaatccccgt ccccagagaa ataatattta
     2401 ttaaaatggc aggtcttctt tttctgatta ccgaagacaa agtggcaatt acgtatagtt
     2461 tcccgataaa gacgcgacag ctacgtcccg cataaggcat ttttctcttt atctttgtac
     2521 ggtacttcat ggaacagaat ttttgacctt gtgaatcgtg atatctgttg gggaggggca
     2581 atttgctcac tgaagcgtga gactcgatta agcgcacgaa acacagaaat caaaaaaccc
     2641 ggccactttt ttacaaagtg gccgggtaaa aataattttt attttttaac tgtcctgagt
     2701 ctcatagaga tgtctcaaaa ctaaaatttg gctcctctga ctggactcga accagtgaca
     2761 tacggattaa cagtccgccg ttctaccgac tgaactacag aggaatcgtg agaacgaggc
     2821 gaatattagc gatgcccacc cacaatgtca aagcctgttt tttaaatttg aaatcgtttg
     2881 ctgaaataat ctgcattttg tcgtttattc cgacacaact ggcttttttt cacacttttg
     2941 cggctcggat cgagggtatt tccacagcca acgtccagta accattcgcc agtaaaacag
     3001 cacggcccgc acagcccagt cggcaaacat tcccatccag acaccaacca caccccagcc
     3061 aagcatgatt ccctgcacat aaccgactac aacccggcaa ccccacatgc tcaacataga
     3121 aacccacatg gcgtaacggg catcacgagc acccttaaat ccagcgggta gcacccatga
     3181 ggcggaccaa ataggcataa ataaagcatt tagctaaatc agaatcacaa cgacgtgttt
     3241 aacctgtgga tcctgggtgt aaaacgatgc cataaccccg gcaaagggag ccgttagcca
     3301 ggcgatggcc gttaatccaa gagtggacag ccagaacaca tgccgcaact gaatctctgc
     3361 ttgcgctatc tgccctaccc ccaaccttcg gcctgtaatg atcgtagaag cagaaccgag
     3421 cgcacttccg ggtaagttga taagagccgc aattgagaac gcgataaaat ttccggcaat
     3481 aacgctggtc cccatcccgg caacgaacat ttgggttaat aaccgaccac tggtaaataa
     3541 cactgattcg acactcgctg gaataccaat ccccatgact tcccagataa tgctaaaatt
     3601 cagcggttta aaatagctct ttaacgaaat ccttagcgca ggattaaaac caatcgccag
     3661 cacccacaaa attgcaactg cgccaatata acgagaaatg gttaaaccca acccagcccc
     3721 gaccaggaga aaaggccgta aatcaatatg ccgctaataa taatattaag aatattcagg
     3781 ctaccgttaa tcagtagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca
     3841 ataagagtga tggcagcggc tggataactg agtacggtca gctccagata agtcaacgcc
     3901 agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa
     3961 tgatgaatta gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc
     4021 gtcgccaccc tcgctcgtcg tcgatcccgc ttaccgagac taaatgctac aacgacagta
     4081 gtaccaagat cgatagctgc aaaaaaagcc ataatgacca tattgaagct gtccgccaac
     4141 cccacgccgg ccatcgcatc ttttcccagc caactgacca gaaaagtgct caggaccccc
     4201 atcaacagga cacaggcgtt ctccatgaag ataggaacag caagcggggt tatctcgcgc
     4261 tagaacaaca ctttgtagct cttgcgttta gagtgccagc gagtgccgtg aacaacctgg
     4321 cgtaaagcag aggagatatt caaagccgac cttaattgca gaaagtgaaa ccacatttca
     4381 aatgatgagg gagaatcagc aaagctgcaa agattttcgc caacaaattg tctgcaaatg
     4441 caacaaactg ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg gtttgacaaa
     4501 atatttttcg ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa
     4561 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaatttctg
     4621 aaaagcccgc ttttatagcg ggctttttgc tatatctgat aatcaatttc ctcttcactg
     4681 ttttccatca cctggcgctt gatatcctca actgacagtc ctgcattaca aagttccaga
     4741 aagcgccaaa catagttacg ctgaagttgt cctcgcttca gtcccaacca gacagtatta
     4801 gcatcgaaaa gatgccgcgt atccaggcgg attaaattct cttcctcttg ttcgccgctg
     4861 gattgttcgg caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca
     4921 gaatcctgtg cacttaatac aatatctgcc ggcaaacctt tgcgggtaaa tgcgtcatca
     4981 gtgcgtgagc gccccgtaat tccctgtcgg taagtaatta acggccactt cgctattgat
     5041 tccagcgtca atggtgtaat ttgcgttaag ggatgatcga gtggaacaag caaactatgg
     5101 tgccaacaaa accatgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg
     5161 ataccaatat cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg cgaaccctgg
     5221 attagctcga gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag
     5281 ctataacgtg cctgagtatg cgtcgttgca atagtgagaa caccagacgt atcgttggta
     5341 aacaggtctg caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc
     5401 agtaatgctt tgcccggttc agtcatcccc agcagtcgct tacctcgtcg aacaaatatt
     5461 tcgatgccaa attcatcctc cagttcccga atatgacggc taacgcctga ctgtgaggta
     5521 aaaagcatat tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc
     5581 tttagttgtt ggaaattcac ggtaaactcc gggcagttca gatatcccgt tattgttaaa
     5641 gtctaatgcc cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg
     5701 cttagctcac caattgccac tgcctttttt ccatcactgg agaactaatc actgacatta
     5761 acaactcttt cactgcctgt gcctgtggtg acaagtttgc tctggcgggt aaatttaagg
     5821 acaaagagag actcattgaa ggagtggtaa tgcgtgacat ccacccattt actgcgccac
     5881 ataacgaacg cgcagccgat tcaggtaata ctgcaacgcc catgccgctg gcaatcgctg
     5941 cggtaagcgt ggcaatagac tcaatttcac caatgacttt tgccgtgagc cgccgtaggg
     6001 aaaaagcctc atcaacacga agtctaacag cgctgtaatc actggggaga aaaaggttca
     6061 tttgcgcaat agcattaaca tcaacgtttt gccccgggca atcttgagtt cctaccagga
     6121 aaagatcttc tttcagcaaa gcctgactgg ataccccagc cacaggggaa tgctcataaa
     6181 tcaccgccat atcgagttgg tgatttatca gtttttcgtt aagcactgca ccactatttt
     6241 catgaagata gataacgacc tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg
     6301 tgatggatga cgcagcggtt cctggcgcaa agccaatcga gacttgcccc gataatgact
     6361 gaccaacgtt atgcaccgcc agttgggcct gctcacactg acgtaaaatg gcccgtgcat
     6421 gggtatagag aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa
     6481 gctgttgatt taactcacct tccagtatgg caacctgttg gctgagcgct ggttgtgcga
     6541 tatgcaatac ttcagcagcc tgggtcaggc taccaatatc tacaattttt acgaagtatt
     6601 tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa tcggcccaag tgccgccatt
     6661 acttacaacc agtatgcaag atgcctgcca gttctatttt ggcgttgatg cgcaagctaa
     6721 ccaactgtca aataagagat tatgatagat tcgtcattgg ccctttttat cagcagtcgc
     6781 attcacctgc cctataaaag gagggtatgc accacgatgg ttcattaccc aataagattg
     6841 aaagctcacc actttgttga aattggcagc aaacaaacaa aaaaaatgca tttcaccctt
     6901 tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct ctgtagttca
     6961 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     7021 caaattcaaa aaagcctgct ttcgagcagg ctttttactt tctaattacc aacgctctta
     7081 aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgat gttgcaacgc
     7141 agcatttgcg cgatttacat caacttcttg cccgttgata aacgcccgca aagatggggt
     7201 taccggcaat ggcacttttc ggtcagactc atattctgca cgattgcgcg acagcggctc
     7261 atgaacttcc agccactttg aaccatccgg ttccgttgtg tatttcactg gctgatcaat
     7321 aatttgtaca cgagtgccta caggaacatt atcaaacaga tatttgatat cgtcattacg
     7381 cagacgaata cagccctgac ttacacggag cccaatacca aaattggcat tggtaccgtg
     7441 gatggcatac aacctgccaa tataaatcgc gtacagcccc atggggttat cgggccccgc
     7501 aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccgga tgttcggcgt
     7561 tggcgtccag gttggtgctt cttgtttacg ttcaacggta gtcacccagt tacgcggggt
     7621 ttctcgccca gcctggccga taccaatagg aaagacttcc actgtattac tgtctggcgg
     7681 atagtaataa agacgcattt cagcgacgtt aaccacaatc cctttacgaa cagtggcggg
     7741 caaaatcagt tgctgcggaa tggtgagttg tgagccaggc ttcggcaaaa aaacatcagc
     7801 gcccggattc gcttccagca tattacttaa cccttgcccg tattgtgcgg caaaagtctc
     7861 cagcggctga gtattgtgat caggaacagt tacagtaagc gactgcccca ctaaacggct
     7921 accctctgga ggtaatggat aagttaccgc caggctattt tggctggcaa aaagcagagc
     7981 aaatgagcaa agaatattta cacgacgcat catgtccctt tcctaagtcg cgaatactat
     8041 ccgataagta tagcttttat cagacttttc gtttttagct gttcaaatca gaagtcgtat
     8101 tccccggtag aacaatatta ctggcagcaa gttcgcccat gttgttgtat atcgcacaag
     8161 cagcttcgat gatggacatc gccagagctg caccactccc ctcacctaaa cgcatatcca
     8221 tattgagata aggctccagc cccaaatgcg agagcgctat acgcgcgcct ttttctgccg
     8281 acaagtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag gctgcgagcg
     8341 cagcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa gcagcaccta
     8401 acatcactcc agctattccg accaaatcaa atccacccac ttttgccagg acatcaacac
     8461 catcctgagg atttggttga ttcaacgtaa tcgcccgacg cacaacatca attttattag
     8521 ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga tcccggccag
     8581 tgattgtgct gactattgcc gctgccggtg tcgtgtttgc catccccagt tcacctacac
     8641 caaacagcgt gacaccgttt ttcgccagct cccgcgtata acatatgacg tccaaaagca
     8701 acttttcagc ctgacggcga ctcattgccg gagctgaagc aatattgccg ctacctcgtg
     8761 cgacacgcat gttgataagc ccggggatag gctcagcagt atcaatacca acatcaatta
     8821 cgtggacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt ccacgggtca
     8881 tattttcagc ctgtatggct gtcacttctt ttggagaaat agcgacccct tcctcccaga
     8941 cgccgtgatc ggcacacata accagtaccg cttttttgcc cacatgcggt atgccattca
     9001 cccccggcat tcctgccagt tgtatggcaa gcgcctccag ctttcctagg ctaccaacag
     9061 gtttgaataa cccgtcaaca tgccgttgtg cacgcgacat agcggcagaa ttgatggctg
     9121 ggatcgtatt cagtaaatcg gcaattattt gcatgtcacg tttctttata gcagtgccag
     9181 caggaagacc agttcaccaa gctctatggc tgctcccagc gtatcgcccg tttgcccgcc
     9241 cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca ccaccatagc
     9301 agccacacca tgcataccca gcaataatac ggcagcaaaa atagcggcta agccgagtgt
     9361 gacgcaggtt tgtcgcccat caattttgcc aatgaacaca ttgccaagcc cttcttcccg
     9421 cgcataacga tgacgataca tcaataatgc ggcagtcccg cggctgaccg cacatgctgc
     9481 cgccagcgat gcaaggatcg gttcgccacg cagagccaac tcgcttaaca ccagaatctt
     9541 tgccagtacc acaaaaatca atgccagacc gccgtgggtg cctaaacggc tatcacgcat
     9601 gatttccaac atgcgatcgc gactacgtgc agaaaatacg ccatcacagg tatcagcgag
     9661 gccgtccaga tggaattccc cggtcatcag cgccagcaca agtacgctaa acagtgccgc
     9721 cagtggtgcg ccacaccatg cctgcagcac catgaagacc agcccgctaa tcgcgccaag
     9781 caataatcca atcaaaggaa aagtaataat accgcgagaa taatgttcga aatccagtcc
     9841 ctgggaccag cgacgcggta caggcagacg cgtaataaat gagagcatcg cccaaaataa
     9901 tttgctcatt tgatttttac tccaataccc gaaaccacca gccatacttc atttgccgca
     9961 gcggccaact gctgatttac ccgcccggca atatcacgaa aatgtcgtgc cagacgactc
    10021 tccggcacaa tccccattcc cacttcgtta gtcactaata caacctttgc ggggcaacgt
    10081 tggcaggcag caatcaacga ctgaatctca gcattaatcg cctgttccat cgcctgataa
    10141 tcccattcat cagggtcttt atcgccgcca taatcaaaca acagattagt caccattgtg
    10201 gtaacgcatt ccagcaacac agcctcattc gggttaatgt ctgcatgaat taattcatca
    10261 agatgttgcc agcgctcca
//
LOCUS       DAJTRM010000123        10218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRM010000123 DAJTRM010000000
VERSION     DAJTRM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.123"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(149..711)
                     /locus_tag="OO322_003526"
                     /pseudo
     CDS             complement(149..711)
                     /locus_tag="OO322_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(915..1121)
                     /locus_tag="OO322_003527"
     CDS             complement(915..1121)
                     /locus_tag="OO322_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5908265.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1228..1791)
                     /locus_tag="OO322_003528"
     CDS             complement(1228..1791)
                     /locus_tag="OO322_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5908266.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3166..5001)
                     /locus_tag="OO322_003529"
     CDS             complement(3166..5001)
                     /locus_tag="OO322_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908267.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5102..5389
                     /locus_tag="OO322_003530"
     CDS             5102..5389
                     /locus_tag="OO322_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5908268.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5361..6890
                     /locus_tag="OO322_003531"
     CDS             5361..6890
                     /locus_tag="OO322_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5908269.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7060..8277)
                     /locus_tag="OO322_003532"
     CDS             complement(7060..8277)
                     /locus_tag="OO322_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5908270.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8644..9630)
                     /locus_tag="OO322_003533"
     CDS             complement(8644..9630)
                     /locus_tag="OO322_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908271.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9627..10155)
                     /locus_tag="OO322_003534"
                     /pseudo
     CDS             complement(9627..10155)
                     /locus_tag="OO322_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3180 a         2339 c         1919 g         2780 t
ORIGIN      
        1 ttaatttaaa ctgtaataat ttaattttag gatgtgtagc agcagcccga tacagagttg
       61 caaggacaca cttttgccag agggcgttac aggaaagctt aatgtttgat tctgtataca
      121 taataaatca ccttatagtt acaataggtc aaaaaccgct gtagccaaag ttacgctggc
      181 ctgatgcttt agtgtcgagc ttcgtcaaat aatccagacg taccaataag cgctggtact
      241 gctctgggaa attaggatcg ttaatatcca ggatgtaacg tccattagca gggaaatgaa
      301 ctaatccctg gtgatcttcc agttccaacc ctaaggcact gtaccaggct ccggtaatta
      361 accctctcag ggtgtcagag cgctcatagt ttcccagatg gtaataggcg tctttgttaa
      421 aaaaaggcag acgtgataat gacatttaaa ggactgcgta tattctttcg cccagataat
      481 acaaaccgac gttgcgtagg tgcgtttgcc ttgttgtttt ttacggtgtt gatctgcatt
      541 aattttcgca ttcagcgagt tacggaaacg agatatctct ccggggatca ggttagggaa
      601 acaggtaatg atatccccgt tgtcaatcac aggagggtaa tggagatccc cccgtattgc
      661 tgttattctg gaagtgtttt taatattcgt attgatagtt ctcagagata tatcacactc
      721 tagatatttt aattaagtat cagttcgata tcggcatatt gtacactacc tcaggtagat
      781 gatgagatca gatacaggga tcaactgacg gtgagcgcag tccccacatt ttatccaggg
      841 cttactgcat attcccaaca gccattcgtt agccaggtag acgaataccc tgatttgccg
      901 ctggttttgc tttcctatcg aagggcccat acatcatcac gcccacgaaa caaccgacga
      961 aataacgcaa ctttctcatc cgtggataat acgaaaacgc actgcacagg gctctgcggt
     1021 ttacgtcgcc attcaatccc atgagcttca agtaatgaga ccagccgggt attttctgcc
     1081 cgcagtactg ctagttcatc gctgtcagat ttataaacca tatgcgttat cacttgtacc
     1141 tgtctcccgg cggcaccacg actgaaattc gtgtgaaggc aaatccacgc gataacttac
     1201 gatagttcgg acataagccc ccataattca gttatgtcgt ggtcgacccc ggttactttt
     1261 ttcgggaagc ctgctacggc ccagaataat accgtgttct ttctgatcct gtttagccag
     1321 atagctaagg gcgtaacgta agtcgtctac cgcagattta tcactatagt gaatcacgtg
     1381 atcgatacgc accgggtatt tgtctttagc ccggcacaga tgaaaataac cctccccttc
     1441 cgtgatccgc ctccagatat ctcccagttg cctggatatc tgataagagt tcttgtgacg
     1501 ttgtccatcc aggtagccga taaaatgaat atgaagccct ttgtctggtg tatattccat
     1561 tacccagtaa tatcccgcca acatcgtctg ggtttcgcta agtaaacggt atatttccat
     1621 acacatactg tgcttacagg aatgcccgaa actgggcgtg tctttcctgt aagcaaaatc
     1681 aattctgaaa ggtaacagtt tagaaaaacg ttgaaacata ccatccatgt gttcattcac
     1741 gtctttcaga atcatgaaat ccattttgta attgggattg gcattataca ttttatagtc
     1801 cttactttat aaaactcaca ggaagagaga attacgtaaa tactgagagt agagaaacgc
     1861 cgtaccacgg cattaataaa cgaagtaaac cagcagcaca gacaacctga taaaactgtt
     1921 ttgtattacc gcattactgt gatgaggtaa tacttcagaa tacagtgaga ctctcttaaa
     1981 ctgagaacca gtacgaatta caatggaaaa acacacctca tagaattgag tttcaggaag
     2041 caccactaaa ttctttacag gaagtaatct gacattcaca aaactcagta tcggctagct
     2101 acaggcaaca tcgctattaa tattgtcgaa ccattaagta aagccgagag agaccacggg
     2161 tatttaatca ttagcataaa agaataatgg tatacagaac atacccacac accataagaa
     2221 gattgttcca tacagaaaag atcatattta cctataccta taaactgtaa cggagtcata
     2281 acacgctcta atagtgttcc gatacgtaac cagtgttaag tacgtacagg acaacattac
     2341 acacataatt caccatacag cttcccccat atgcttcact gcgtagtgta tggagatata
     2401 tgcattacac acatacagaa tacttagcat aaagaggagt gacattacca cgtaaagaga
     2461 taagttatac aacaaacata caaacaatac ggcacagaca cacaatacca taactccaac
     2521 taataatact aattaacctt aattatttat ggtatatgaa ataaatgtga ttctcccgga
     2581 actaactaac ctaaccagac agatgatcac tgtataaaaa cttcagttaa tactcatttg
     2641 ttttgtcgac tcagtatata atgagtgacc agtaatcagg ataatcacac ttacataacc
     2701 tgtaaaaaag aacaagaaga ataagccact aacataatgc aaaatggcat ctgaatcgat
     2761 cgcagtttgg cattcgaatc gatcatgatt tggcccaatc cgtgatcgaa tagagcggaa
     2821 tacgcaacat aattcaccag acaagagctt acaccgaatt acagattatg tattaatgtc
     2881 aaaactggca tattttacag atacaatgaa catgcaaccc atcaatctaa taggggcggt
     2941 atgtacatct gcccatgaat gggatggcgc taagaataca aaacaattag acatagatca
     3001 caaaaacaca ccattctaat tgaaaaacaa aacaaacaaa taaatcatac tattatagaa
     3061 ttttctcttg ccagacatag ataatacccc tgctaatttt gctaatggtt agcaaaatta
     3121 gcagggtata taaagattat tgacccaaaa cacactcaga tgaattcacg acagctcagg
     3181 agggttattc ctgatgtcat cttcacaaat aatggttaac gcatcatata tcattctgcg
     3241 tctgttcctg ttttcggcct tatcgccata caacatcatc acatttttaa tttcatcgac
     3301 cggtgatttc acatagtgaa ctaatacacc aagcttctca acacattgca gtaaagtata
     3361 tttcttactg cctgtgaaaa gccctatcat agtttcttta cgttcgcgac tggataatac
     3421 tttgttttcc ataatgactt ctgccagcgt cttaccatca taaacatact ctttatctat
     3481 cagaatattt atcttatcca gcaccttctc tacgtcatca agcaaacctg taatatatgg
     3541 gggcgcaata tatggtttcg tactgatctt ttcaagcata caattataca tcttaataac
     3601 acgcatcagg gtaaaattgt tcgcatcata ctgtatttca ccctccttat atacaggatc
     3661 aataagtggt attttttcaa taaatgcatc ctgataggct tttacatggt caagccaata
     3721 aaacaacgag ccatacttta tattttttct atttacttta acctccagtg ctgtccgaag
     3781 caacgataat gcccccctga catatcctga aggtaattca ccaaacaatc cttttctttc
     3841 catgtcatca taatgctgca gcacatcatt aattttattt ttccttatca gacaaatgta
     3901 caccgcaagt gtttcgtagc agctggtcac atgaacagcc tcattctgca actgcccaaa
     3961 atagcttata atctttttca gatatgcgtc atcacaacca ttcttatcat taatattaaa
     4021 tacaagatct ttcagacggg aaatatattc ctgagattta tcatcaagtg aaacactata
     4081 acgagacaat gttccttctg cactgttttg aaacttataa aacatttcca gtaatttttt
     4141 tgcataaggc gttgtatcaa agctggtaaa gtcataaaga tattgcgcag cgtaaccata
     4201 caaaccatga atccagagtc cttctctcac caacactctg agataatatg caaggctttg
     4261 aagtatcttc gtctttcgtt gtatatcaag taagacactt aatgaataaa atgcattata
     4321 gaatttatca atattcctga gcactttctc cagttcatcc ttatccagct tcttctcccg
     4381 gtaatccttt ttatattcat ttttgatagc catgatatat tgttgcatca ggctcttgct
     4441 tttcccgtta tccttccgta tagttcctgt atatttcccc agagcataca gctttccatt
     4501 ttcatgaaaa tcttccttgt tgaatcccag ttcagccaga tattcccgaa gataacgttt
     4561 aacagcagct tcatcgcttt ctgtatcagc gtgatcaaga caaggacaat acgatttctg
     4621 taaaaaatta taaatatgat aataaaaagc agagtcattc tctggtctgt atgtattata
     4681 catatctaca acaagaaaaa taaccccaaa agtgcgcaga caatagctgc gaaggaattc
     4741 aggttttagt gtggaatgca ctggataaga attcatcatg taataaaaat gttcatatcg
     4801 ggcatatatg atccgccgtt gctcctgact gaacacccct tcagcatcaa taatacgact
     4861 gatctcatta taacgttgat cccagaattt ttgatgcatt tcaaaattgg tactcttccg
     4921 cttgtgtgcc ggattctcgc cttccttaaa caaccccaaa acagtacaaa tatcatacaa
     4981 catatttgta aatgtgcgca tatgctgtgc tctccggttg gtttgcattt tatcgcagag
     5041 gacattctga gcttaacgta tcaaactgac acaacgacat gcaatgcata tggagaacaa
     5101 tatgtcaaat caaaccggac agctttacac gccttcacgc atcctgcgta tggctgaact
     5161 tacccgactg ctgggtatat caagatctac tatttacgtg aaaataaatc cggcctcgaa
     5221 ataccacgac cctacctttc ccaaaccaat aaggcttggt agtgcatccg taggctggcg
     5281 ggcatctgcc atcgatgagt ggctgatgtt acatacggca ccagcctggt ctgaacctga
     5341 aaacaaacta aaggaactta atgatgacga caaaactatc actccctgaa cttgacacaa
     5401 ctaaactgcc tgacaggtgc caggcattac ttgatgaaat gcatgaagaa acaggaatac
     5461 gccgtgaaat acttctgtct gttatgctga cggtcatggc tgcctcggtc caggatacgc
     5521 atgaagttga actttccgga gggcaacgta ccagccttca gatatttatg tgtctttcat
     5581 cagcatcagg cagtggaaaa acctctgcct gcgcaaaatt aatcgctcct gttcatgaaa
     5641 cagaagaaga actacatcag gcatatatcg acgataaaaa aaactatgac cgaatgatgg
     5701 aaatgtggac aacagataaa aaaatcctgg agcggagata taaaaaggaa attgaaagat
     5761 ccccggaaaa tgctgctgct gcacgagcag cacttgaaaa gtgtatagca aataaacccg
     5821 aacctcccgt acaacaggtt ctcatcgtga atgacgcgac accagaaggg atagctttga
     5881 aactcagcca gtcaccctct cttctgttgc tgtctgatga aggaggtact attctggata
     5941 agcgtttcga acgcaaatcg gcactgtaca acacattatg gagtgggcaa ccagtcaacg
     6001 ttgaacgggc atccagaccc gggttccggc tgaatgatgt ccgtctcact atacttatac
     6061 tgactcaacc ggtaatattt aataagtttt ttactctcac tggcgatcag atccggggca
     6121 atggctttct ggcccggttg ctattttgcg aacccggagc aattaaaata atgacgacag
     6181 aaccgcatac tgccactcct gttgtgacgc aacaatgtgc aagctgtttt cgtaagaaaa
     6241 gttttggcag tcagatcaga gactctctga gagcatccag agaaagacgt gcaaaaagta
     6301 aagagcgcat ctgcatgaca ttatcaaatg cagcatcccg cgcactggag cgctttcaca
     6361 aagaaaatat ggatactgtc agacagaatc ctcatatggg tacatttgaa gacattattg
     6421 tcagaaaaag agagcaggtt gtcaggatag cagcacttct ggaactggag aaagatccag
     6481 actcgacagt aataacgctg gaaagtacta atagcgccat ttatctcatt gaattttatt
     6541 tcaagcatct tatatataaa cttgaatccc ttcgggaaat atccccggct gagaaacttg
     6601 ataaatggct tcagaaaaga attatcacaa cagcaggtta catattccag aaaagttata
     6661 ttttacaata tgccccatat gctctcagga aaaaatgtgt tctggatgaa gcacttgaca
     6721 tactggcgga acaaaggaaa ataaggattg atgataacct ggttgtttat ataggaaaca
     6781 cgattacacc atccgaactg gcaaaaaaac taaatattcc tgcattcgat gccggagttt
     6841 tcatttgtga tcatcaaaac atactgaagt atcaccgaaa cagactatag atactatcgg
     6901 acaaaaaata aattatcaca agtgtattat tcgttataat aatgaataat cgttatctga
     6961 gtgcaatgcc aggttttcat tacgaactgg cattgcattt atttttcaaa aaagaaagca
     7021 gtaaccattt ggctactctg agtatgccag tccatgaaac taagcgaatt ccttagtgtt
     7081 tttagcaagt tgagacatca cataatccgc ccatgcctgc atcatcggta cacgctgctc
     7141 cagtagatca gtacgatgat atgccgcctc aaccttattt ttcagcgtat gagcgagcgc
     7201 cctttccgcc agatcccgcg aatacccctg ttcactacac cagtccctga atgttgagcg
     7261 aaaaccatgt gccgtggcaa ctcgccccgg aatgtcactg acggctttct ttttacgcag
     7321 aaaacttgtc aacaccatat cggaaaggat ctgctgcttt ctgggtgaag ggaacaccag
     7381 ttcatcatgc aggccacgta tattttccag aatgtaaata gcctgccggg ataaaggaac
     7441 acgatgctgt agcctagctt tcattctttc tgcaggtata gtccataccc gcttatgaaa
     7501 atcaatttca gcccagcgca ttcccctggc ttcgcccgag cgagttgctg taagtatcac
     7561 cattaataac agtgcgcggg taacattata aggttcatca ctatacacac tggtcgccac
     7621 aaaaagcggt aactgcctcc agggcattgc gggttggtgt tcatcacgtc ctcttgtctg
     7681 ctgaggaagc aaatggtcaa ccacatcaac aggatttgcc acacaaaaac cgtgcgccca
     7741 tccccactgc ataacaacat gaatgcgctg tttaacccgg cttgccgttt ctgacaaggt
     7801 taaccagact ggacgcagtg tttctgccac gtccgcagcc gtaatcgaat ccagcgtttt
     7861 tgctcccagt tgaggaaacg cgtaattctc aagcgtcgat aaccactgcc ttacatgctt
     7921 tggattctcc catccaggag acagttctgc atgtacacgc ctggctgcat cggcaaatgt
     7981 tgggataacg actttctcag attcagcctt tttaatctcc agaggatcat cacctgcagc
     8041 aagttgctct cgcattatcc gggcagtacg tgcagcttca gcaatactga cctctgggta
     8101 agttcccaat ccagcattac gtcttttttg tgtcaccgga cttacataac gaaaaaccca
     8161 tttcccccgc ccctttactg aagaaggatg aagggtcagt ccggtaattc ccccatgggg
     8221 caatggtttg tcatcaggtt tgatatgtct tgctttcgta tccgtcaata ctgccatacg
     8281 ctaattcccg tcactctggt atgccatcca gtatgccatt agtccctcgc ttcactaaga
     8341 atacattaga taacatcgga caacaaaaag tgtaatacat tgtttataaa gaaattatat
     8401 aacacccctg gattaagtca gatttatttc aggcggtccc cctcaccgcc atatttaaag
     8461 aagagcgcgt acgaaagtac gggctttttt ttcgtatatt gtacacaccg ggtgcccccg
     8521 cccacgtctg ttagggcgag gggaaattgt gcacatcaac tgtctggtta ctcagtcaac
     8581 aatcaaaatg cgtaagatag tacagaacat atatgcgtta tctggtcact tcctgtaact
     8641 tcctcactta ttaaaatgcc cccaacggaa atacatgtca catgccatca taaaatcgtt
     8701 atgccagagg aatcgacaat ggaaaaaata gtaaattaag cacggtacat agcagagtaa
     8761 aaaataggct tcaaaccaaa tccgccattc cacaagcgtt gccaataccg acaccagcgt
     8821 aatccacatc atttcaataa cccgcaatat gaataaccga agccaaagcg gcttggcaaa
     8881 aaaggtcgac aacgtatttt cactggagaa aaacagatcg atttcctgca aaaaaaacct
     8941 gcccagaaaa gcgggccgct tggagaagcg cagaaaaaac agattaatcg ccaacactac
     9001 gccacaaaga atgagcaatg caattatcga tttaaacgtt aaccactgcc cctctgtcac
     9061 aaaagaatca ctgttacgca gcgggctgat agtaagcagg ttaacaaccg ttgagatgca
     9121 caccgaaagc agcacatagc gagtcagact tttatatttt acttttttcg ctactgaaat
     9181 agcgacctgc ttgacataac tggtgcggat catccgaaac agcagaaaga tgaccaacgg
     9241 cccaagtaac gaaatcagga acagcgcata taacgagggt agaagctggt atagccccca
     9301 actcattgca ctcaacatga ttaacgacag aaatcctgcc accagaggtt gtgtaagaaa
     9361 taattctatt ttgtggttct gctttttctt aaaccacgaa aaatcgatat tcttcgtgac
     9421 gcatttataa gcccagtacg ccttgagatc aaaaataaaa ttatacagca ccagggccag
     9481 aatcccacta actacaccaa tatcctctga tggaaaagta aaccctgcat ttttccaata
     9541 aaagcctatc agaataagtc caatagcata aatgtatatc agatttcgtt tatcctgaaa
     9601 gcgaataaaa gttaaataat caggaatcat ttgaatacct ttgcatcact aaatgcttta
     9661 cgacaatgca taactcgttg ctgatccacg ccagcaaaat catttaacat attccccaaa
     9721 tagggcttat tacttatttc aaggaaagct accggggttg gcatattcag aatatgtatc
     9781 gagagattca gtccatttca ccgtatgagt taagtgtaat gagagatttt ctttgataat
     9841 tgacggagcg acctcgcttt ttgctgttac gttcatcaga acctgatgct ctggcgaagc
     9901 gatatccagt ccggcaagat aatcgcgcat tgcctgaacg ccgtcttcca tcaaacgcgt
     9961 gtgccaggca ccgctcacac cgagtttaac cggttcgtat ccagcggcca tcagcagcgt
    10021 ggtaaattca ttcaacgcag cctgcgttcc cccaataacc tgctggcgcg gcgtgttatc
    10081 acagctaata tccagcgcaa tgcctgattc cgtaatcatc gtctgcagtt gttcgcggtt
    10141 gatgccttta accgcctgat cttacccagc aatagtggac acgcggctaa gtgagtaaac
    10201 tctcagtcag aggtgact
//
LOCUS       DAJTRM010000124         9937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRM010000124 DAJTRM010000000
VERSION     DAJTRM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.124"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO322_003535"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO322_003535"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5908272.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO322_003536"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO322_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5908273.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO322_003537"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO322_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5908274.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO322_003538"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO322_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5908275.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO322_003539"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO322_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5908276.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO322_003540"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO322_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5908277.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO322_003541"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO322_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5908278.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO322_003542"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO322_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5908279.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /locus_tag="OO322_003543"
     CDS             complement(8990..>9937)
                     /locus_tag="OO322_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5908280.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2587 c         2295 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttcct
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAJTRM010000125         9920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRM010000125 DAJTRM010000000
VERSION     DAJTRM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.125"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..317)
                     /gene="psiF"
                     /locus_tag="OO322_003544"
     CDS             complement(<1..317)
                     /gene="psiF"
                     /locus_tag="OO322_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5908281.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(436..1851)
                     /gene="phoA"
                     /locus_tag="OO322_003545"
     CDS             complement(436..1851)
                     /gene="phoA"
                     /locus_tag="OO322_003545"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5908282.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1952..2212)
                     /gene="iraP"
                     /locus_tag="OO322_003546"
     CDS             complement(1952..2212)
                     /gene="iraP"
                     /locus_tag="OO322_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5908283.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2394..2597)
                     /locus_tag="OO322_003547"
     CDS             complement(2394..2597)
                     /locus_tag="OO322_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908284.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2675..3769
                     /gene="ddlA"
                     /locus_tag="OO322_003548"
     CDS             2675..3769
                     /gene="ddlA"
                     /locus_tag="OO322_003548"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5908285.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3793..4005)
                     /locus_tag="OO322_003549"
     CDS             complement(3793..4005)
                     /locus_tag="OO322_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5908286.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4265..4573
                     /locus_tag="OO322_003550"
     CDS             4265..4573
                     /locus_tag="OO322_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5908287.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4632..5726)
                     /gene="yaiW"
                     /locus_tag="OO322_003551"
     CDS             complement(4632..5726)
                     /gene="yaiW"
                     /locus_tag="OO322_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5908288.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5739..6959)
                     /gene="sbmA"
                     /locus_tag="OO322_003552"
     CDS             complement(5739..6959)
                     /gene="sbmA"
                     /locus_tag="OO322_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5908289.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7311..8468
                     /gene="ampH"
                     /locus_tag="OO322_003553"
     CDS             7311..8468
                     /gene="ampH"
                     /locus_tag="OO322_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5908290.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8469..8972)
                     /locus_tag="OO322_003554"
     CDS             complement(8469..8972)
                     /locus_tag="OO322_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5908291.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9060..9587)
                     /locus_tag="OO322_003555"
                     /pseudo
     CDS             complement(9060..9587)
                     /locus_tag="OO322_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9607..>9920)
                     /locus_tag="OO322_003556"
     CDS             complement(9607..>9920)
                     /locus_tag="OO322_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5908292.1"
                     /translation="LVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFF
                     QLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGS
                     V"
BASE COUNT         2460 a         2536 c         2347 g         2577 t
ORIGIN      
        1 gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt cagagcttgt
       61 tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa acttttttca
      121 ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg agcatcccct
      181 ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg ttgtggggtt
      241 aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag caaggtaacc
      301 agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc gtttgcgccc
      361 gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag cgaaaattca
      421 ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga agagatcggt
      481 ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa tacgcaactg
      541 actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca ccatcactgc
      601 gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg gcgcaacaac
      661 ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct cctttttagc
      721 gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa tttgcccaca
      781 agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca ggaaaaatcc
      841 tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca gggttggtac
      901 actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga tattgccgtg
      961 gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa acaggccaag
     1021 caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat cgttcaccaa
     1081 ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt caccagcggt
     1141 tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac gagcgttaag
     1201 cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg gacatttttc
     1261 actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca gcgcagcggg
     1321 cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg cggcttttgc
     1381 catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg cgccgttata
     1441 ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga cgtagtccgg
     1501 tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg gtaaggcatc
     1561 gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag taatttccga
     1621 atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat cgctaagaga
     1681 atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg gtgcagtaat
     1741 atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg cttttgtcac
     1801 aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca ctttattttc
     1861 tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg gccgtgacaa
     1921 ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc aataacttct
     1981 ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg ggctttacct
     2041 cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc gccatattgc
     2101 gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt tctttcgact
     2161 cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc atgtctgtat
     2221 ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata gtgtctggaa
     2281 gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg attgccagcg
     2341 aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa ttatcaacct
     2401 ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt aagagaaaaa
     2461 aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat catttttgtg
     2521 atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca cgctttgtca
     2581 gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt
     2641 atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg taggaatcgt
     2701 ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga
     2761 tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac aagggcaatg
     2821 gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc atattgcgtt
     2881 gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc agcttatcga
     2941 cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg tccacggtac
     3001 gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac cgtttgtagg
     3061 ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac gtctgctacg
     3121 cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc gtcacaacat
     3181 cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac cggctaatca
     3241 gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga
     3301 tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat
     3361 cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg agatcgtact
     3421 caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg cgaaagtggt
     3481 agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg ccgttcaggc
     3541 ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa ccccagagaa
     3601 cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca gtatgtatcc
     3661 gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt tgattgagct
     3721 ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat attttaacgc
     3781 ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc
     3841 cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt
     3901 tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc caatggcgaa
     3961 cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag ctccgttaac
     4021 gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca acattttcgt
     4081 aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa gattcacgct
     4141 tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct aaatgacctg
     4201 tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt
     4261 ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc
     4321 ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc
     4381 gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca
     4441 ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat
     4501 cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc actggcgaca
     4561 tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc tggcctgaca
     4621 ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac
     4681 caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc aggcaacatt
     4741 tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt cttgtacagc
     4801 gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat ttcgctgtcg
     4861 ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc gggttcttta
     4921 ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc cttactgact
     4981 gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc agcaaagcgg
     5041 tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac
     5101 ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca cggataccct
     5161 ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt gcgcaccgga
     5221 ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc gtcaaaaatc
     5281 gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact tttgccgttt
     5341 ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat ccgttcagca
     5401 cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg atctgcctga
     5461 taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag cgtcggtgcc
     5521 agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg
     5581 ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc gccctttttc
     5641 aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa caatgacagc
     5701 gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt atgggttact
     5761 tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt gtagatagac
     5821 atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga
     5881 ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac
     5941 ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgggcgatg
     6001 ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc gctaaacagc
     6061 tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac cagctcttta
     6121 cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt gatccctacc
     6181 actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac
     6241 ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac cagcaccggc
     6301 aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt ctccagcgtt
     6361 gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat atgacgcagt
     6421 tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga
     6481 ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat ccccagaaag
     6541 acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt
     6601 tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac ttccaccaaa
     6661 aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg ccaacggtgc
     6721 gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa
     6781 aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg
     6841 ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac ggcaatcaat
     6901 gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata
     6961 tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg catgaccgct
     7021 acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc
     7081 agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg
     7141 gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta
     7201 cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg
     7261 agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg ttgaaacgta
     7321 gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc
     7381 cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt
     7441 acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc
     7501 gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc
     7561 gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg
     7621 ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccga
     7681 cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc gccctgcccc
     7741 gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac
     7801 gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag gcggcgtact
     7861 ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata
     7921 cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc acctacaccc
     7981 cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata
     8041 acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct
     8101 ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc
     8161 agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca
     8221 aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgagga
     8281 ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga
     8341 agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga
     8401 gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg
     8461 catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt
     8521 tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg
     8581 cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag
     8641 tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata tcccgtacaa
     8701 tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc
     8761 ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata
     8821 actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa tataacttgc
     8881 ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca
     8941 aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt aatgcccgtc
     9001 aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag tgaagaatat
     9061 taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc accaccaccg
     9121 aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga
     9181 ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta
     9241 tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctgatct
     9301 tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa
     9361 ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga
     9421 ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg
     9481 gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta agtaccgttg
     9541 ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagctatg aatcgccacg
     9601 gataatctag acacttccga gccgttgata atactggttt tcatattctg tcggtgacat
     9661 ctgttcgcta gaaccatgcc gacgcttact gttataaaac atttcgatgt aatcaaaaat
     9721 atcactgcgg gcttcttccc gcgttccgta gatctttttc tttatccgtt cacgtttcaa
     9781 caactggaaa aaactttctg caaccgcatt atcatggcag ttaccgcgac ggctcatgct
     9841 accctccagg ccgtgtgatt tcaggaacga ctgccactca tggcttgtgt actgactgcc
     9901 ctgatccgaa tgaaccagca
//
LOCUS       DAJTRM010000126         9885 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRM010000126 DAJTRM010000000
VERSION     DAJTRM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.126"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO322_003557"
     CDS             complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO322_003557"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5908293.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO322_003558"
     CDS             complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO322_003558"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5908294.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO322_003559"
     CDS             complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO322_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5908295.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4682..6442)
                     /locus_tag="OO322_003560"
     CDS             complement(4682..6442)
                     /locus_tag="OO322_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5908296.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6572..7198
                     /locus_tag="OO322_003561"
                     /pseudo
     CDS             6572..7198
                     /locus_tag="OO322_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7397..8485
                     /gene="serC"
                     /locus_tag="OO322_003562"
     CDS             7397..8485
                     /gene="serC"
                     /locus_tag="OO322_003562"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5908297.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8556..9839
                     /gene="aroA"
                     /locus_tag="OO322_003563"
     CDS             8556..9839
                     /gene="aroA"
                     /locus_tag="OO322_003563"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5908298.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2517 a         2364 c         2475 g         2529 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca
      361 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
      421 agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
      481 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
      541 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
      601 tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
      661 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
      721 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
      781 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg
      841 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
      901 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
      961 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
     1021 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
     1081 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
     1141 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
     1201 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
     1261 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
     1321 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
     1381 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct
     1441 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
     1501 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
     1561 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
     1621 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
     1681 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
     1741 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
     1801 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
     1861 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
     1921 gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact
     1981 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
     2041 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
     2101 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
     2161 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
     2221 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt
     2281 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca
     2341 taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg
     2401 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
     2461 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
     2521 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
     2581 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
     2641 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
     2701 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg
     2761 tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
     2821 agtcaccgat gatacggcca cggccataag catctggcag accggtcaga acaccggatt
     2881 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
     2941 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
     3001 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
     3061 tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga
     3121 tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa
     3181 caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
     3241 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
     3301 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
     3361 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
     3421 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
     3481 cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg
     3541 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
     3601 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
     3661 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
     3721 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
     3781 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
     3841 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
     3901 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg
     3961 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
     4021 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
     4081 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
     4141 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
     4201 tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
     4261 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
     4321 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
     4381 catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa taaattttaa
     4441 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
     4501 agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat
     4561 ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa
     4621 aaacgccccg taatataact cagactaatc attaagccta cattgcgtag gctatttgat
     4681 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg
     4741 actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg
     4801 cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa
     4861 acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat aacaacgttt
     4921 gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta aactccatcg
     4981 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
     5041 ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
     5101 gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
     5161 ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
     5221 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
     5281 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
     5341 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
     5401 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
     5461 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
     5521 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
     5581 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     5641 cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     5701 catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca accaccgctg
     5761 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     5821 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg
     5881 tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     5941 aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt
     6001 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     6061 cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc agtgggaacc
     6121 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     6181 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     6241 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     6301 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     6361 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc
     6421 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     6481 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     6541 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     6601 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     6661 tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg
     6721 tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc gttttatgat
     6781 gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt ataccgcctg
     6841 gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga cggaatttga
     6901 gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat
     6961 tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg
     7021 tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg agtcggcgga
     7081 ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa aacaattatg
     7141 tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca
     7201 gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat gcaagccaca
     7261 tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata aaaccgatag
     7321 ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc
     7381 aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg caatgctacc
     7441 ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc ttggtacgtc
     7501 ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg aagccgagaa
     7561 ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct gccatggcgg
     7621 tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa ccaccgcaga
     7681 ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa aatactgcac
     7741 gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta agccaatgcg
     7801 tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg aaaccatcga
     7861 cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg ccgccgactt
     7921 ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa tttacgctgg
     7981 cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg aagatttgct
     8041 gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca acgataacgg
     8101 ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg tctttaaatg
     8161 gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa aagcagaact
     8221 gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga aagctaaccg
     8281 ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat tgttccttga
     8341 agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg
     8401 cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag acttcatggt
     8461 tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac agccagcctg
     8521 tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg ttacaaccca
     8581 tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct aaccgcgctt
     8641 tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg gatagcgacg
     8701 acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg ctttcagccg
     8761 atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa ggtgccctgg
     8821 agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg
     8881 atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg attggtcatc
     8941 tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa gaaaattatc
     9001 cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat ggctccgttt
     9061 ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa gatacggtga
     9121 ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc aatctgatga
     9181 agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta aaaggcgggc
     9241 agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg gcttcttact
     9301 tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca
     9361 gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg accatttgct
     9421 ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat atggatatga
     9481 accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca aaaggcacca
     9541 ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg
     9601 caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac attcgtatca
     9661 ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac cggatggcga
     9721 tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat cccaaatgca
     9781 cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag gcagcctgaa
     9841 tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgt
//
LOCUS       DAJTRM010000127         9740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRM010000127 DAJTRM010000000
VERSION     DAJTRM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.127"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            71..202
                     /locus_tag="OO322_003564"
     CDS             71..202
                     /locus_tag="OO322_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5908299.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            462..1457
                     /gene="pgl"
                     /locus_tag="OO322_003565"
     CDS             462..1457
                     /gene="pgl"
                     /locus_tag="OO322_003565"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5908300.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1498..2451)
                     /locus_tag="OO322_003566"
                     /pseudo
     CDS             complement(1498..2451)
                     /locus_tag="OO322_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2635..3687
                     /locus_tag="OO322_003567"
     CDS             2635..3687
                     /locus_tag="OO322_003567"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5908301.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3763..5196
                     /locus_tag="OO322_003568"
     CDS             3763..5196
                     /locus_tag="OO322_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5908302.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5379..7559
                     /locus_tag="OO322_003569"
     CDS             5379..7559
                     /locus_tag="OO322_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5908303.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7700..8983)
                     /locus_tag="OO322_003570"
     CDS             complement(7700..8983)
                     /locus_tag="OO322_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5908304.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9118..>9740)
                     /locus_tag="OO322_003571"
     CDS             complement(9118..>9740)
                     /locus_tag="OO322_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5908305.1"
                     /translation="FREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAES
                     SPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALG
                     ISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHEL
                     RSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2375 a         2283 c         2478 g         2604 t
ORIGIN      
        1 catggaagga gatcacccaa ttctcccccc cgacttttga cgcagtgtct tgttaacagg
       61 gggattgacc atgcttattg gctatgtacg cgtatcaaca aatgaccaga ataccgatct
      121 acaacgtaat gcgtttattt gtgccggaag tgagttgatt gttgcgcgaa tgggaagttg
      181 gccagaagtt gttatggcat agtgtttacg gtcgttgata caggtaaaca aaagcgggga
      241 gagtcatact ccctgaatag tcgcgaaaat atctgtggca ttgtcaaatt cgtgtcggtg
      301 actaatattt tagtaatatg ccattcgaaa tggagtgcta acttattgat ttaattgatg
      361 tgcgatattg tattttatcg tgttttgttg tctgtttttt atccagtaag tgttgttata
      421 tgtatgaaag ataagggatt tatcattttt gaggtgtgtt catgaagcaa acagtttata
      481 tcgccagccc tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga
      541 cgctgacaca ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca
      601 aacgttatct ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtattgccc
      661 cggacgatgg cgcactgacc tttgccgcag agtctgtgct gccgggtagt ccgacgcata
      721 tttccaccga tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga
      781 gcgtaacgcg tctggaagat ggcctgccag tgggcgtcgt cgatgtagtc gaggggctgg
      841 acggttgcca ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa
      901 agcaggatcg catttgcctg tttacggtca gcgatgatgg tcatctggtg gcgcaggacc
      961 ctgcggaagt gactaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg
     1021 aacaatatgc gtattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag
     1081 atccgcacgg caatatcgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg
     1141 acacccgttg ggcggctgat attcatatca ccccggatgg tcgccattta tacgcctgcg
     1201 accgtaccgc cagtctgatt accgttttca gcgtttcgga agatggcagc gtgttgagta
     1261 aagaaggctt ccagccaacg gaaacccagc cgcgcggctt caatgttgat tacagcggca
     1321 agtatctgat tgccgctggg caaaaatctc accacatctc ggtatacgaa attgttggcg
     1381 agcaggggct actgcatgaa aaaggccgct atgcggtcgg gcagggacca atgtgggtgg
     1441 tggttaacgc acactaaccg ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta
     1501 acttatctcc tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt
     1561 atcgtcagtc cacaaattaa tcagcaagga aaaaaagcgt tcagctgccg gagaaagcac
     1621 ggcatctttg cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtgggat
     1681 accaataatc gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc
     1741 cagtcctgcc tccactagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc
     1801 cagcttccag ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc cgctggagga
     1861 acgtacgcca atcattttgt aacccactaa ctcctgccat tctacaagct actttttggc
     1921 caatggatgg tcacgccgac aggccagcac gaatggctcg ttgaccagtg gggtaaaatc
     1981 aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac
     2041 cgattccatg caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag
     2101 ctcattaaat ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc
     2161 taatgtcacc ataccacgat gataagcatt catatcacga atattaaaaa gcgtttcatc
     2221 gaatttcttt attaattccc gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt
     2281 aaccttgcga gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat
     2341 tcttcgcgtt aatgcaggtt gcgtaatatt gagtaattta gcggcattgt taaatgaact
     2401 ggactctgcc agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc
     2461 ggaaaatgga agcgacgatt ttggggggct ggtcgttaaa aattttaact gcatttagcc
     2521 aacttaaatt aatgaaaaaa tgttattaat cgttgagcta aagtcattag agatgctttg
     2581 tccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa
     2641 aaaataccct gcgtgatgat gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa
     2701 catttacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt
     2761 aacgatctgg aaattgacgg tattggcggc ggtaatccac tgaccagtaa agtcgccatt
     2821 attagccgtt ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca agttatcgtc
     2881 catgagcaac gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg cgtaggggca
     2941 ttcgccattg aaaatggttt gattgcagcg acttcgccag ttacccgtgt acgtatccgc
     3001 aacgtcaata cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag
     3061 tacgagggta gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact
     3121 ttcctgaatg ccgctggaac gaaaactgga aaagttttcc cgactgataa tcagattgat
     3181 tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg
     3241 gctgaatatc tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca
     3301 ttattagccc gcattgaatc tatccggcta caagcgggta aagcaatggg cttaggtgat
     3361 gtcagtaata tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca
     3421 attaatgtgc gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct
     3481 attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct
     3541 gtaggatatg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt
     3601 aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacaac cagaaaaata
     3661 ttttccggtg aagtttatct tccctgaaaa aattcgttgt caggataagg acaatcaata
     3721 aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta
     3781 tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca
     3841 ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtgggtctg
     3901 gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg
     3961 gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct
     4021 tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc
     4081 ggtttaggta aacttattgc ctatctgctg attggtaaaa tcggtaacac catgctgggt
     4141 ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc
     4201 gcgcgtgcgg gcggcattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc
     4261 gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc
     4321 accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa
     4381 atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct ggccgccgga
     4441 ttgccgggca tcattatgct gctggtcacc ccgctggtaa tttacaccat gtatccacca
     4501 gaaattaaaa aggtggataa caaaaccatc gctaaagcgg gccttgccga actgggaccg
     4561 atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact gggctggatt
     4621 ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg
     4681 ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc
     4741 ttaatctggt acggcggtat tatcggctta agctccttat tatcgaaagt taaatttttc
     4801 gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct
     4861 ttcttcgtta ttattttcct cagcatcatc gtgcgttatt tcttcgcttc cggtagtgcc
     4921 tatatcgttg ctatgttacc tgtatttgcc atgctggcga acgtctccgg cgcaccgtta
     4981 atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat
     5041 ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa atcctggtgg
     5101 ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct cggcgtgtgg
     5161 tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata cttcaagttg
     5221 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggatt
     5281 cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt tattatcttt
     5341 ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag
     5401 gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta
     5461 aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct cataatacgt
     5521 ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct
     5581 ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg ctgccgtatg
     5641 tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac ggtgatgacc
     5701 atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct ccgcatattg
     5761 cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg atcctcgggt
     5821 cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag ggtggcggtg
     5881 agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc gtggttgcgg
     5941 tgcatctgac cggaaaacct gcgccgtatg tggagccaca ggatgtggcg ctggctatca
     6001 ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc gttggaccgg
     6061 gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc acggaaacca
     6121 cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg gcgctgcacg
     6181 gtcgcggtca ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca
     6241 tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg
     6301 tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag attgaaattg
     6361 agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa gtggaaaatg
     6421 gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg
     6481 tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg
     6541 cggtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt gtggtagcag
     6601 atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc tttggcgcgg
     6661 gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc
     6721 gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt
     6781 ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa cttgattgct
     6841 gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt gtttatcagg
     6901 gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt aaagactggc
     6961 cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg
     7021 aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc
     7081 cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga
     7141 ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggtagcatg gtctatgcgg
     7201 tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt gtgattggcg
     7261 gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac gtcatcaact
     7321 gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg gattacattt
     7381 acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa ggttatgtga
     7441 tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg acggctgaag
     7501 agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa
     7561 aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat gctggcgcat
     7621 cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta cgccgcatcc
     7681 ggcaaatagt taatagctct tacttcttct cctctgcaac cactttgctg cccacgccgc
     7741 ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt
     7801 cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc accgcatcgg
     7861 cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac
     7921 cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca tcaccggaag
     7981 cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc gtagccggtg
     8041 caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg gtgttatcga
     8101 acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag ctgttggtca
     8161 ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg ttggtgacaa
     8221 agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacttgg tcaccgtcag
     8281 tacgcagtgc taccgccgga tggttacctg catctacgct atcgcccagc gtgttttcga
     8341 tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca gaacacagaa
     8401 caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac
     8461 ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc
     8521 catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtga
     8581 ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc atcacggcaa
     8641 tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg
     8701 tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg
     8761 ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgcg
     8821 catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcag
     8881 tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa
     8941 aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat
     9001 gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc attataaaaa
     9061 agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat aaaatcatta
     9121 tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg atgccatggt
     9181 gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct tctcatagag
     9241 tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga aggaaagacc
     9301 tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa gcggttcacg
     9361 acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat caagtgtttc
     9421 cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa tttttacgcc
     9481 tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact tcatttcgca
     9541 taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc tgagccaaca
     9601 tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa gtcttgatct
     9661 ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta tatggccttc
     9721 agctattgcc tctcggaatg
//
LOCUS       DAJTRM010000128         9608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRM010000128 DAJTRM010000000
VERSION     DAJTRM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.128"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            382..2013
                     /gene="oppA"
                     /locus_tag="OO322_003572"
     CDS             382..2013
                     /gene="oppA"
                     /locus_tag="OO322_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5908306.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2099..3019
                     /gene="oppB"
                     /locus_tag="OO322_003573"
     CDS             2099..3019
                     /gene="oppB"
                     /locus_tag="OO322_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5908307.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3034..3942
                     /gene="oppC"
                     /locus_tag="OO322_003574"
     CDS             3034..3942
                     /gene="oppC"
                     /locus_tag="OO322_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5908308.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3954..4967
                     /gene="oppD"
                     /locus_tag="OO322_003575"
     CDS             3954..4967
                     /gene="oppD"
                     /locus_tag="OO322_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5908309.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4964..5968
                     /gene="oppF"
                     /locus_tag="OO322_003576"
     CDS             4964..5968
                     /gene="oppF"
                     /locus_tag="OO322_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5908310.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6021..6350)
                     /locus_tag="OO322_003577"
     CDS             complement(6021..6350)
                     /locus_tag="OO322_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5908311.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6385..7845)
                     /gene="cls"
                     /locus_tag="OO322_003578"
     CDS             complement(6385..7845)
                     /gene="cls"
                     /locus_tag="OO322_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5908312.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7988..8161
                     /locus_tag="OO322_003579"
     CDS             7988..8161
                     /locus_tag="OO322_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5908313.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8216..9469)
                     /gene="kch"
                     /locus_tag="OO322_003580"
     CDS             complement(8216..9469)
                     /gene="kch"
                     /locus_tag="OO322_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5908314.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2505 a         2257 c         2397 g         2449 t
ORIGIN      
        1 ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga gaatcgctta
       61 acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc gggcaaccgc
      121 cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata
      181 atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca
      241 gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca gcagaaagtc
      301 tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat
      361 aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc
      421 gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc
      481 acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca gtcattagat
      541 ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta
      601 ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg ggataataaa
      661 gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga tggcacgcca
      721 gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct
      781 ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg
      841 aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt agaagtcacc
      901 ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg
      961 ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc
     1021 aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc
     1081 ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct
     1141 tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac gtataacaac
     1201 atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat
     1261 ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg
     1321 cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa
     1381 gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg cgcaaaattg
     1441 actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg
     1501 ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg gtataacacc
     1561 tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt
     1621 gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggc
     1681 acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac ttccttcctg
     1741 aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac
     1801 agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact
     1861 aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat
     1921 gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc
     1981 tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct
     2041 gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat
     2101 gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta ttcttattac
     2161 tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg aacgtacttt
     2221 accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac
     2281 acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat cgtttaaata
     2341 taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg ccaaactggg
     2401 agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt
     2461 aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat
     2521 ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt tgcattggct
     2581 gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt
     2641 gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt
     2701 acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt
     2761 acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc ccgcatttgt
     2821 cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg ggattgggca
     2881 actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct
     2941 ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga
     3001 cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag
     3061 acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca
     3121 cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct ggtgctgatc
     3181 gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg
     3241 gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc
     3301 ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat ggtcggtgtt
     3361 gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc cggttatctg
     3421 ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc
     3481 atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct gattttcgtg
     3541 gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt
     3601 ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac gccgggcatt
     3661 gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc atcgctactg
     3721 gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag
     3781 ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga agtctctcca
     3841 tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt caactttatc
     3901 ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg
     3961 taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga
     4021 aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttaa
     4081 atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt tcgggtaaat
     4141 cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt ggcggatcgg
     4201 caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat aaactgcgcg
     4261 ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg
     4321 tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt
     4381 ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga
     4441 aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt gcgatggcat
     4501 tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg
     4561 tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat actgccatca
     4621 ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt
     4681 acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa cccgttcatc
     4741 cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt gaaacaatgt
     4801 tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc ccgttccagc
     4861 cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc
     4921 ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaactga
     4981 aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa tcaaagatgg
     5041 caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcactcttcg
     5101 cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt
     5161 tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct ggttaggtaa
     5221 agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat
     5281 tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga tcatcgctga
     5341 accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc gcgtgaaggc
     5401 gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc
     5461 cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat
     5521 tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg tggttaacct
     5581 gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc
     5641 cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cagtagaact
     5701 ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc
     5761 ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg
     5821 ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat
     5881 tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca gacacgccgt
     5941 ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg tcagcccttt
     6001 ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt
     6061 tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc actgtccgcc
     6121 agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc
     6181 cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac
     6241 agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc
     6301 tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc
     6361 tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa acagtcgctc
     6421 ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg
     6481 cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc
     6541 caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag
     6601 acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata
     6661 aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc agccgaccag
     6721 catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct gcgccgccgt
     6781 gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata
     6841 ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc
     6901 ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt
     6961 gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata
     7021 aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg
     7081 ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta
     7141 cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa
     7201 aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat
     7261 tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg
     7321 gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt ccgccatgcc
     7381 gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct ggatgtcgcg
     7441 gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat tccctttgac
     7501 cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt
     7561 ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg
     7621 ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag
     7681 ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga
     7741 gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta
     7801 tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt
     7861 ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg
     7921 aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt
     7981 agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag
     8041 ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat
     8101 caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta
     8161 aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt aaactctatt
     8221 tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatac
     8281 catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta
     8341 aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta
     8401 ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac
     8461 tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg
     8521 ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt
     8581 caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt
     8641 caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc aggatcgtat
     8701 tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat
     8761 tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca
     8821 tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac aatcgtgctg
     8881 attcagaaac agggacaata tctccatagc cgacggttga catggtttct atcgaaaaat
     8941 agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac
     9001 cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg
     9061 cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc
     9121 aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt agcgctatca
     9181 acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt
     9241 tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca
     9301 actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata ccatagattg
     9361 ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg
     9421 tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg
     9481 taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg
     9541 tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagtgagc
     9601 aactctgc
//
LOCUS       DAJTRM010000129         9541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRM010000129 DAJTRM010000000
VERSION     DAJTRM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.129"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            290..652
                     /locus_tag="OO322_003581"
     CDS             290..652
                     /locus_tag="OO322_003581"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR5908315.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            649..1566
                     /locus_tag="OO322_003582"
     CDS             649..1566
                     /locus_tag="OO322_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5908316.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1579..2844
                     /locus_tag="OO322_003583"
     CDS             1579..2844
                     /locus_tag="OO322_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5908317.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(2911..3534)
                     /locus_tag="OO322_003584"
     CDS             complement(2911..3534)
                     /locus_tag="OO322_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5908318.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4285..4623)
                     /locus_tag="OO322_003585"
                     /pseudo
     CDS             complement(4285..4623)
                     /locus_tag="OO322_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            4688..5146
                     /locus_tag="OO322_003586"
     CDS             4688..5146
                     /locus_tag="OO322_003586"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013043.1,HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5908319.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            5278..5538
                     /locus_tag="OO322_003587"
     CDS             5278..5538
                     /locus_tag="OO322_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR5908320.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(5628..7625)
                     /locus_tag="OO322_003588"
     CDS             complement(5628..7625)
                     /locus_tag="OO322_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5908321.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(7625..8920)
                     /locus_tag="OO322_003589"
     CDS             complement(7625..8920)
                     /locus_tag="OO322_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5908322.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQFIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALT
                     ARGQDIQIRGQNISAQNAALSREIQRAELQEKALDRQIARESNQLKLEELKQKQADVR
                     QKAEIARADRQAAAQGAVDTFSTALDSLNEIEQSPGLSKAVGIRSAFPTVPGSDAANF
                     EARLDTFKAQTFLPMVQSLKGMGALSDAEGKKLSDAVGALSPKMSEKAFRDSIGKIRN
                     QLESKLSTVKKQFDYQEPVQNTPGQQSPAGSNFSSLWGD"
     gene            complement(8930..>9541)
                     /locus_tag="OO322_003590"
     CDS             complement(8930..>9541)
                     /locus_tag="OO322_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5908323.1"
                     /translation="SDKSAKYAAEAQKYAADLQNQQWQTIMKNLAPFTPLAEQYVNQL
                     QNLSSLEGQGQALNQYYNSQQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPT
                     LGQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAAIAAANANKPSS
                     LQTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT         2538 a         2191 c         2048 g         2764 t
ORIGIN      
        1 ctgtagcacc aatcataact atctgtttta gcaagcatta accgcattca ctaagatata
       61 gttgatggca caaacagaaa gttgatgaat gatgttgcca tatatatgtc acaaatacgg
      121 cacaacgatc ttcaaacata tagccacaca ttcacagaag agcacaaagc cttgcaatcc
      181 agtgcaaaga tttgtgtgcc agagtttttc tcatcaacta ccgcaagtat cgatcgattg
      241 agacttggat gatagacttc atgcctttga ttattagctg atagaagaaa tgttaaagct
      301 atttgcaaag tacacatcta taggcgtgct gaacacactt atacactggg tggtttttgg
      361 tgtatgtatc tatgccgcgc ataccaatca ggctatggca aacttcgcag gtttcgttgt
      421 ggctgtgagt tttagcttct ttgcgaatgc aaaattcaca ttcaaagcat caactacaac
      481 gatgcgctac atgttatatg tcgggttcat gggaacactg agtgctactg ttggatgggt
      541 tgctgataga tgttcacttc ctccaattgt cactcttgtt accttctccg ccatcagctt
      601 ggtgtgcggt ttcgtctatt caaagttcat tgtctttagg gatgcgaaat gaagatatct
      661 cttgtcgttc ctgtcttcaa tgaagaagaa gcgataccaa ttttttataa aacggtacgt
      721 gaattcgaag agttgaagcc atatgaagta gaaattgttt tcataaatga cggaagcaaa
      781 gacgctacgg agtcaatcat taacgctctg gctgtttctg atccgctagt tgttccgctg
      841 tcatttacac gcaactttgg taaagaacca gcattgtttg cagggttaga ccatacaacc
      901 ggcgatgccg tgattccaat tgatgttgac ctgcaagacc cgattgaggt tatccctcat
      961 cttattgaaa agtggcaggc aggtgctgac atggtgcttg ctaaacgttc agaccgctca
     1021 actgatggac ggctgaaacg taagacagct gagtggttct ataaattaca caacaaaata
     1081 agcaccccaa aaatagagga aaatgtcgga gattttcgac tcatgtctcg tgaggttgtg
     1141 gagaacatta aactattgcc tgagcgcaat cttttcatga aaggcatact gagctgggtt
     1201 ggtggtcaga cagatgtcgt tgaatatgta cgcacagagc gtgttgctgg catctcaaaa
     1261 tttaatggct ggaaattatg gaatctggca ctggaaggta tcacaagttt ttcaaccttt
     1321 cctcttcgcg tatggactta tataggcttg tttgttgcaa gcatttcatt tttatatggt
     1381 gcatggatga ttatagacac cattgtcttt ggtaacccag tacgcgggta tccctccatg
     1441 cttgtatcaa tacttttcct gggtggagtg caactgatcg ggattggtgt tctcggtgaa
     1501 tatataggta gaatttattt agaaacaaaa tcaagaccac ggtacttaat taaaagcaga
     1561 aaataacagg atgatcttat gaagatattc ataatgcgca attggcacaa gatatcaatt
     1621 tttatacttg catttacttt gatttggctt agacgcattg acattttgac aaatgcccag
     1681 ttttgggctg aggatgcagt tttttggtat aaagatgcat acgaacaagg ttttttcagt
     1741 tcactcataa ccccaagaaa tggttatttt caaacagtat caacactcat tgttggtgcg
     1801 acgacattta ttaatccaat ttacgcacca ttattatcaa acttttttgg aataataatt
     1861 agagctataa ttatatggtt cttatttacc gatagattta aattcctaag caccacatca
     1921 aaaatcttca tctctgtata tttaatttgc atgcccgggc ttgatgaagt ccaagccaat
     1981 ataacaaatg ctcactggta tttatcattg tatgttgcaa tgataataat atctgaagaa
     2041 tccaagtcaa aattatggaa agcacatgat ttatttttta tagtattatc tggattgagc
     2101 ggtccattta ttatattcat aattgcatca tctgttttta aatatttaca cttatcaaaa
     2161 ggagtaataa gcataagagg tttatactta ttttatactc gactgccata tttggcaatg
     2221 atcttgtgtg ggctgataca ggcaacatca ataattctta catttaatgg aacaagatca
     2281 catgctccat taggattcag ttttgatgtt atgtcatcta taatatcatc taatgtattt
     2341 cttttttcat ttgtcccttg gaatatcgct aatgctggat gggacaatca ccttttatcg
     2401 tacacactat caactttagt aattacatta tgtgttttca tttatattaa aggaaattgg
     2461 caaatgaaag tgtttgccac ccttccaata cttattgtcg cattttctat ggcaaaacct
     2521 caactggcag attctgttcc tcaactgcca acacttgcaa ccggaaacgg gtcaaggtat
     2581 tttgtaaata ttcatattgc aattttttca ttaatatgcg tttgtttgtt tcaatgcata
     2641 aagaataaaa ccttaaaaat attctttaaa atctatgttt cagtcatcct tattgcaatg
     2701 ataaaattaa acttttttat aacccctctt cctgacatga actggtctca aggagcagaa
     2761 ttaataaata aagcaaaaca tggagaagcc gtctcattta cagtgctccc tccatggtta
     2821 actcttgatt taataaaaaa ataaaacttc acattaataa ataacaatga atccgcctcc
     2881 ctacgtattc ccgtagggag gctgtcgtgt ttatagtagc ctggtaatgc taaatgcaaa
     2941 cgaactagag attcctgctc cgctaagcgt attgcgcagg gtcaccctaa atgtatctac
     3001 gccaatataa gtaacatcac aactaactga atcaggcgca gatataggtg ttatctgcac
     3061 aatataaccg ctagcaactg ctgcgagcgg atgagaaaag ctaacctcat acacacctgt
     3121 agcaaccacg ttaaccgatg aaacaccagt agcaacgtcg atactaccat tggcgttaat
     3181 ttttcccgag cgtgtgactg acaatgcaga agccgatgtt gccttactgg ttgtttctgt
     3241 aaaaacacac ccaatgtcaa ctacctcgca attagctcct gttatccagt atggtcgatc
     3301 tgcacttggc tgataatccc ctgcgctctg gaacgttgtg ccgttgagta tgactgtaac
     3361 ttttccccca cctgagcttg ttacttgaat attagtgtgc gtgtatcttg cactgctcac
     3421 gcgatgaaaa tttccaccgt ttataaccac agttacaggg cgcgtcccag tattatctat
     3481 agcaaggtct gcccccccgg cattagcctc aaagtatggg ttgtttaata ccaaagcgca
     3541 cgaaccgtta aggccattta tggctaattg cgcaccacct gtactggaat ccccctgagt
     3601 accatttcct tcacaagtca ggctattgat agttgtacca gcaccccagc ggtccccgag
     3661 gataccccat tcactgttct gactgcacgt aagcctattt gcagttatag cgttcagttc
     3721 agaatgattt acggagttgg tagcacgcca accgatacga ttactctgca agtacacgtt
     3781 cgtcatgtcg ctcgtgagca tcgagtcagt gcgtaaccct tccccgtcag ggtggcggca
     3841 gataacaatg ttttcaagac gagtatgtgc tttattctgc accagcatac cgtaacttcc
     3901 gggattccct ttcagcgtca tgttgccgat gtagtcatat gcagccaatc tctgactgtt
     3961 aaacgctgcg tcacctagca tttttacgtt aaaatcagta gctctacatt ccagtattgt
     4021 ctcagccatg ttctcgcctg agtaaaaaca gcggacaccc ggagcaccta caaagtcggc
     4081 agggtaatca tcataattaa gtaccagagg agtggtaaca tcatacaccc cttgctttat
     4141 ttttaatgtt ccaccgaaag gccacgatgt aagcgggtct tgatatattt cattttttat
     4201 tgcaaatagc tcatcttgaa gatttttccc gctattagtg ccaatgtaag tagccccttc
     4261 atcagattga agtaactgct ttaactgatc cgggtcatac ttcagcaaat tcggaaaata
     4321 gaactgttgc gaaccatacg catcatatac agccattgaa tggccttgct cagttacaaa
     4381 tttggcaatc tgcccgttgt acacaggata tccggcagca ttgataacaa ttggctgaga
     4441 aacagggacg tgagatccgt cttcattctc tacataaacc tgaatctggt tttcaggatt
     4501 taccgggtca gtgtcaattt tcccgatata aatttttcca ttggcatttg ctttaaacga
     4561 gcgagctaaa gtaaataact gacttggttg tgaaacaaca acgttgtata attgttctgg
     4621 cataataaac attccggaga aaataatgag taagagacgt cacgaggtag taactcggga
     4681 gcgccttatg gaggttctct attacaataa agaaacagga attttcactt ggaaaaagaa
     4741 attaagcgcc agaggtgctg ttgggaaagt tgctggcaca atttcttatg gatataacgc
     4801 aattaatatt gatggcgtaa gatattttgc acatagactt gcctggctgt atgtatatgg
     4861 cgaatggccc aaacaagaga ttgaccacat tgatagaaac agaagaaata acgcaatctc
     4921 aaaccttagg gatgtcagca gagtcgtaaa tgcattaaat gtcggacctc gaaatagcaa
     4981 tgctggaatc aagggggtaa ctttctgtca agccagaaat caatggcaag ctcagataaa
     5041 tgtctcaggt aaaaacataa cccttgggag attcaacacc attgatgaag cggctatagc
     5101 ttataaggca gcaaacatgg ttgctgacca tctgttgagt aggtgatatc tgacatttca
     5161 ttgctccaga cgaatgatat gatgcaacca tgatgtgatt gcataccgaa atggtactat
     5221 tgagtattta tccagtaagt tacgatgcca ttccacccaa ttggtgaggc atcaaggatg
     5281 tacagcaaat acgacgaggc gcagtttcac ttgagacttc cgcatgaact ccacgcgaaa
     5341 attaagcagc gtgcgaagat gaataacagg tctctgaact cagagataat tgcagcgatt
     5401 gaagaatcac tggataaaca aaactctgca tcagtttaca ttgacgatgc agagcgtatg
     5461 gcagaacaac aatccgatat ggttaagaaa attgtctttg atacgctcaa agagctatat
     5521 aaaaaagaca gcagctaacc atcagttacg gaggatttat gcaaagagat atgctgaata
     5581 ttgcgttcta catatttggt ttttgcacgt tcctggtgtt tgcgaagcta ttctgacaac
     5641 gcatcagact tggcaccctg agtcagggcg ttaatggcct tttgcgcctg ctgcatggct
     5701 ttctcaaacg ctgttgatcc gcgtggggta tttgccattc ggagcattgc atttctgaat
     5761 ggctcgctct cataggcgcg agtaagaagt ccgtagctta ccgctgcgcc agttgtcgcc
     5821 gggttcattg ccgtcccata cccgataatg aacgggatgg tttgctgccc tgttggtgtt
     5881 gttactgccg cttttgctgc ctgctgagtg gactgaaggt aatttttcaa tcctttcaga
     5941 taagcagcgt cctgcccctt aaatgtgatg ccagtctggt tttgcaggat gttaagctgc
     6001 cgaaggaact ggtcagggga tccgccagat ttctccatcg cctttccaat gatgccattg
     6061 cgcatttgca ccctgccaac acgaccaact gagttataca gcgtcttaat ttccgatttg
     6121 ttcttgctga atagcatgtt gttgacaact tccggcgtca ggtcgccttt catgagaaca
     6181 ttcttcagcc tggtattctt tagtttcgcc gcttcgtcag cgtagacggc attggcctgc
     6241 tgatatttac ggagagtatc gttgccaaga ttctgaccaa tggcaccatt gatatcgtcg
     6301 gtcatcgcct tgtaaacgcg ctgaatggca gcatcggaac ggtttggtaa cactggtcgc
     6361 tcacccttca cgtccattct gaactgactg cgcaggtcgc ttaattgctt caaatccaga
     6421 tttaccggac cttcagggcc agcattgcga acaagctcat cacgatatga ctgaagtttt
     6481 gaaattgtct cgttatcagc caccttacca agcttctgca ggttagatat ttctgtatca
     6541 atctgctgaa ttgctcgcgc aggctgaatg tttactccag ccatagcatt ctgaacctgc
     6601 tccagtcgat taccggcggc acgacgaatt cctgatgttt tcgctttaag gctgtcaata
     6661 acaaccgctg gatcatactc cccgaattta tcagcaaatc tctgcaccaa ctggcttctc
     6721 gcttcctgtt gcgttgctct cattccgctt gtgccagcca gagggatatt ttctgctgta
     6781 gtctgcgcca tttttccgac gcgggaagtt ggttgtaaca ggtctgtggt gtgcagagga
     6841 actccttcac gctctgcaaa tctgatagcc tgctgagctt ctggcgcgat agcaccacga
     6901 acgccacgat aagcagcacc taatccacgt ccggcagcgt taatagcacc gccagcaagt
     6961 acaccaacgc ctaaatcggt ggcgagtgct tccgcatcat ctttcgcact atttgcagca
     7021 agtgatccaa ctgcgttttc agcgagaagg cgagttgcac cctgagcaat tcgaccagca
     7081 agtgttggtg cctgtgttgc cgctctctca acgccggcag gagtgaggta aggcaatgct
     7141 tcagcaaata ccctgccctc tgtcgtttgt ggagtcagcg caccttgctg aaggccaaag
     7201 tcctgctcta atccatgcgt tgttactcgt ggcgctggtt gatatgttcc atcgccaatg
     7261 ccgagtttac cgccagccca tgcagccgcg cttgttacag catcggcaac cgatgcaggt
     7321 atgtttgcca cgttcacgcc agcctgcacc agtccgcgac cagtctcttt tactgcttcg
     7381 ccaagatcag acataaatcc actttgctgt ggttgttgct gtgctactgg ttgctgtgtc
     7441 tccactggct gcacagatgg caatggatag gcagcataga aagcttgctt agcctgctct
     7501 gcattttctc cggcttgcgg ggcaacgact tcattgaagt attgctcctg agcctgcgct
     7561 ttttgttctg gtgctaacgc ctgatactgt ggagatgcga taacatcttt ccatgcttta
     7621 gccattaatc accccatagt gaagaaaaat tactgccagc aggagattgt tgccctggcg
     7681 tattctgcac cggctcctgg taatcaaact gttttttaac agtgctcaac ttgctttcaa
     7741 gctgatttcg aatctttccg atagagtcac gaaaagcctt ttcactcatt ttgggactta
     7801 gagcaccaac cgcatcggat aattttttac cctcagcatc tgaaagagcc cccatcccct
     7861 tcagggactg caccataggg aggaaagttt gagctttaaa ggtgtcgagt cttgcttcaa
     7921 agttagccgc atcagatcca ggaactgtcg gaaacgctga gcgaatacca actgcttttg
     7981 aaagtccagg gctttgctct atctcgttga gagaatcaag cgcagtgctg aacgtatcaa
     8041 cagcgccctg agcggcggcc tgtctgtcag cacgagctat ttcagccttt tgccgaacat
     8101 ctgcctgttt ctgctttagc tcttcaagct ttaactgatt gctttctctg gctatctgtc
     8161 tgtccagagc cttttcttgt aattctgctc tttgtatttc gcgggaaaga gcagcattct
     8221 gtgcgctgat gttctggcca cgtatctgga tgtcctgacc tcgtgctgtt agtgcctcgc
     8281 cagcctgatt gctgcggatt gtctctgcca gcctacctcg gtcaatctca cgaccagcca
     8341 tcttgtcctg aacattgaag taatcaatcg gaccaagagc agccatccca aggtgatcaa
     8401 caaactcacc aaatcctgaa ggattctgct gatacatctg agcaacgttg ttaggatcaa
     8461 cgccgacgcg agtcagttcc ttggcgttgt tttgcagcca tgattgcatt gcttctggag
     8521 acgatgacgc aaggcgtgcg ccagccgcta aggtgccgat agaattacgc tgctcttcat
     8581 caatgaatcc catgccttta cgaacggatt caatctggtc tggatattga gtagccaact
     8641 gacgcaaagc accgcgatca ccagacgcat aagcattagc gtatgcctgc tgaaattctt
     8701 tctgccgctg agcctgcttt tcctgctgaa acacccccgc aatacctgaa aggccttgca
     8761 aagcagtcag cccaacattg ttagcgcctg aacgctcaat atcattgttc tgcctgataa
     8821 actgaagcgt attgccgatg tcatttacgc tcggagcgtt tgagtttacg ccgccgatac
     8881 cagccaacaa tccaccattt gatccttgcc aagtagccat gattacccct taaaacaacg
     8941 agccaagcaa tccgatacca gcaccaatgc cagcgcccca aggtgttgat gttcccaaaa
     9001 ggctggcaag acctgcaccg gcaatcgcac cagacgtgcc accgctaatt gcagtctgaa
     9061 gacttgatgg tttattggca ttagcagcgg caattgctgc actttgctgt gcaatgctgc
     9121 tcatgttgtt ggcgtatgtc tgcccagcgt ttgcctgacc ttgcagcgca ccaagcccaa
     9181 cgtttgccag attattgtaa ttgctcatct ggtttgataa ccacgactga ccgagagtcg
     9241 gagcaatcgt agccagttga ttgcttgtgg ctgtcgaacc aagtcctccc gtagcctccg
     9301 cagcagcaag actctggtaa cgagcctgac ctgcaaggtc tttatactgc tgagagttgt
     9361 aatactgatt aagtgcctga ccctgtcctt ctaaactgga aaggttctga agctggttaa
     9421 catactgctc cgcaagcggc gtgaacggag caaggttttt catgatcgtc tgccactgct
     9481 gattttgcag gtctgcggca tacttctgag cttctgctgc atactttgca cttttatcag
     9541 a
//
LOCUS       DAJTRM010000130         9446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRM010000130 DAJTRM010000000
VERSION     DAJTRM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.130"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            68..1186
                     /locus_tag="OO322_003591"
     CDS             68..1186
                     /locus_tag="OO322_003591"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5908324.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1252..1500
                     /locus_tag="OO322_003592"
     CDS             1252..1500
                     /locus_tag="OO322_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5908325.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1565..1933
                     /locus_tag="OO322_003593"
     CDS             1565..1933
                     /locus_tag="OO322_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5908326.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2027..2680
                     /gene="nfsB"
                     /locus_tag="OO322_003594"
     CDS             2027..2680
                     /gene="nfsB"
                     /locus_tag="OO322_003594"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5908327.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2788..4035
                     /gene="ybdG"
                     /locus_tag="OO322_003595"
     CDS             2788..4035
                     /gene="ybdG"
                     /locus_tag="OO322_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5908328.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4103..5479)
                     /gene="pheP"
                     /locus_tag="OO322_003596"
     CDS             complement(4103..5479)
                     /gene="pheP"
                     /locus_tag="OO322_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5908329.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5580..8723)
                     /gene="cusA"
                     /locus_tag="OO322_003597"
     CDS             complement(5580..8723)
                     /gene="cusA"
                     /locus_tag="OO322_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5908330.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(8735..9253)
                     /gene="cusB"
                     /locus_tag="OO322_003598"
                     /pseudo
     CDS             complement(8735..9253)
                     /gene="cusB"
                     /locus_tag="OO322_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2292 a         2616 c         2321 g         2217 t
ORIGIN      
        1 cttccgcatc tcgcccagcc gctccgactt ctatactgaa tagaaaacgt caacttaaga
       61 gaaacctatg ccattacccg attttcatgt ttctgaacct tttaccctcg gtattgaact
      121 ggaaatgcag gtggttaatc cgccgggcta tgacttaagc caggactctt caatgctgat
      181 tgacgcggtt aaaaataaga tcacggccgg agaggtaaag cacgatatca ccgaaagtat
      241 gctggagctg gcgacggatg tttgccgtga tatcaaccag gctgccgggc agttttcagc
      301 gatgcagaaa gtcgtattgc aggcagccgc agaccatcat ctggaaattt gcggcggtgg
      361 cacgcccccg tttcagaaat ggcagcgtca ggaggtatgc gacaacgaac gctatcaacg
      421 aacgctggaa aactttggct atctcatcca gcaggcgacc gtttttggtc agcatgtcca
      481 tgttggctgt gccagtggcg atgacgccat ttatttgctg cacggcttgt cacggtttgt
      541 gccgcacttt attgcccttt ccgccgcgtc gccatatatg cagggaacgg atacgcgttt
      601 tgccccctca cgaccgaata ttttttccgc ctttcctgat aatggcccga tgccgtgggt
      661 cagtaactgg caacaatttg aagccctgtt tcgctgtctg agttacacca cgatgatcga
      721 cagcattaaa gatctgcact gggatattcg ccccagtcct cattttggca cggtggaggt
      781 tcgggtgatg gataccccgt taacccttag ccacgcggta aatatggcgg gattaattca
      841 ggccaccgcc cactggttac tgacggaacg cccgttcaaa catcaggaga aagattacct
      901 gctgtataaa ttcaaccgtt tccaggcctg tcgctatggt ctggaaggcg tcattaccga
      961 tccgcacact ggcgatcgtc gaccgctaac ggaagatacc ttgcgattgc tggaaaaaat
     1021 cgccccttca gcacataaaa ttggcgcatc gagcgcaatt gaagcactgc atcgccaggt
     1081 cgtcagcggt ctgaatgaag cgcagctgat gcgcgatttc gtcgccgatg gcggctcgct
     1141 gattgggctg gtgaaaaagc attgtgagat ctgggccggt gactaagccg ggaattgcct
     1201 tacagaagaa tatccctgac aatggtgttt taacttacat ttaacaatgt tatgaaacac
     1261 cctttagaaa ccttgaccac cgcagcaggc attttgctga tggctttcct ctcttgcctg
     1321 ctgctgcccg cccccgcact ggggctgacg ctggcacaaa aactggtgac catgttccat
     1381 ctgatggatc ttagccagct ttacacttta ttgttttgtc tgtggttttt agtgctgggc
     1441 gctattgagt attttgttct gcgctttatc tggcgacgct ggttctcgct ggcggattaa
     1501 acgtcgatta tcgccgaact tcgcggcata ctttgcttat tctccttcgc ctaaaggaac
     1561 gtttatggat aagcaatcac tgcacgaaac ggcgaaacgc ctagctcttg agttaccctt
     1621 tgtcgagctt tgctggcctt ttggcccgga gttcgatgtt tttaaaattg gcggcaagat
     1681 ttttatgctg tcgtcggagc tacgcggcgt cccctttatc aatctgaagt ccgatccaca
     1741 aaaatccctg ttaaatcagc aaatataccc aagcattaag ccagggtatc acatgaataa
     1801 aaagcactgg atttcggtgt attccggcga ggaaatctcc gaagcgttac ttcgcgatct
     1861 gatcaacgat tcgtggaatc tggtggttga tggtctggct aaacgcgatc aaaaaagagt
     1921 gcgtccaggc taaagcggga atctatagcg catttttctc gcttaccatt tctcgttgaa
     1981 ccttgtaatc tgctggcacg caaaattact tacacatgga gtctttatgg atatcatttc
     2041 tgtcgcctta aagcgtcatt ccactaaggc atttgatgcc agcaaaaaac ttaccccgga
     2101 acaggccgag cagatcaaaa cgctactgca atacagccca tccagcacca actcccagcc
     2161 gtggcatttt attgttgcca gcacggaaga aggtaaagcg cgtgttgcca aatccgctgc
     2221 cggtaattat gtgttcaaca aacgtaaaat acttgatgcc tcgcacgtcg tggtgttctg
     2281 tgcaaaaacc gcgatggacg atgcctggct gaagctggtt gttgaccagg aagatgccga
     2341 tggccgcttt gccacgccgg aagcgaaagc cgcgaacgat aaaggtcgca agttcttcgc
     2401 ctatatgcac cgtaaagatc tgcatgatga tgcagagtgg atggcaaaac aggtttatct
     2461 caacgtcggt aacttcctgc tcggcgtggc ggcgctgggt ctggacgcgg tgcccatcga
     2521 aggttttgac gccgcaatcc tcgatgcaga atttggtctg aaagagaaag gctacaccag
     2581 tctggtggtt gttccggtag gtcatcacag cgttgaagat tttaacgcta cgctgccgaa
     2641 atctcgtctg ccgcaaaaca ccaccttaac cgaagtgtaa ttctctcttg ccgggcatct
     2701 gcccggctat ttcccctcag attctcctga tttgcataac cctgtttcag ccgtcatcat
     2761 aggctgctgt tgtataaagg agacgttatg caggatttaa tatcccaggt tgaagattta
     2821 gcgggtattg agatcgatca caccacctcg atggtgatga ttttcggtat tatttttctg
     2881 accgccgtcg tggtgcatat tattttgcat tgggtggtac tgcggacctt cgaaaaacgt
     2941 gccatcgcca gttcacggct ttggttgcaa atcattaccc agaataaact cttccaccgt
     3001 ttagctttta ccctgcaggg gattatcgtc aatattcagg cggtattctg gctgcaaaaa
     3061 ggcaccgaag cggcagatat tctgactacc tgcgcgcagt tgtggatcat gatgtatgcg
     3121 ctgctttcag tcttctcgtt gctggatgtt attttgaatc tggcgcagaa attcccggcg
     3181 gcatctcagt taccgctgaa agggatattt caggggatta aactgatcgg cgcaatcctg
     3241 gtcggcattt tgatgatctc gctgctgatt ggtcagtcgc cagcgattct aatcagcggt
     3301 cttggtgcaa tggctgccgt gctgatgttg gtatttaaag atccgattct tggtctggtg
     3361 gcaggtattc agctttccgc gaacgatatg ctgaaactgg gcgactggct ggaaatgccg
     3421 aaatacggcg cggatggcgc ggtgatcgat attgggttaa ccaccgtcaa agtgcgtaac
     3481 tgggacaata ccattaccac tattcccacc tggtctctgg tttctgactc ctttaaaaac
     3541 tggagcggga tgtcagcatc tggcgggcga cgtattaagc gcagtatcag tattgatgtc
     3601 accagtattc gttttcttga tgaagacgaa atgcaacgtc tgaataaagc gcatttgtta
     3661 aagccttatt taaccagccg ccatcaggaa attaatgagt ggaatcgcca gcaaggttct
     3721 acggagtcgg tattaaacct gcgccgaatg accaatattg gaacctttcg tgcctatctg
     3781 aacgaatatc tgcgtaacca tccgcggatt cgtaaagata tgaccttaat ggtacgccaa
     3841 ctggctccag gtgataacgg tttaccgctc gagatctacg cgtttactaa caccgtggtg
     3901 tggctggaat atgaaagcat tcaggctgat atattcgatc acatatttgc gattgtcgaa
     3961 gagtttggtc tgcgacttca tcagtcgcca accggcaatg atattcgctc tctggcgggt
     4021 gcatttaagc agtaattaaa aaaaccgctc tcatcgaatg gatgagagcg gacatcgttt
     4081 tgtcggatgt agcgtgaatg ccttatttcc gacgcagcgt tttaaatgcc acaaacagga
     4141 atacaatcca caccggcagc aggatcgctg acaagcgcat atcatccatc gtgcacatca
     4201 gcagcaaaat catgccgagg aaggcgatgc aaagatagtt gccgaacgga tagagcagcg
     4261 ccttaaactg tgtttcacgc ccctgacgtc gcatcgccgc gcgaaaacgc agatgcgcca
     4321 ggcagatcat aatccagttc aacagcaacg ttgctaccac cagcgccatc agcagaccaa
     4381 acgctttttg cggcagcaga tagttgatta acaccaccag cgaagtgatc gctccggaaa
     4441 gcatcagcga gttaatcggc acaccgcgac ggctgacgcg agtcaaaaac ttcggcgcat
     4501 taccctgcac agaaaggcca aacagcatgc ggctgttaga gtaaaccccg ctgttataca
     4561 ccgacagcga tgccactaga atgacgaagt tcagcgcaga agctaccaca ttgctgtcga
     4621 gattatggaa aatcatcaca aacgggctac tgttggattt cacttccacc cacggataga
     4681 gtgccagtaa aaccaccagt gaaccgatat aaaacagcag gatgcgatac accacctgat
     4741 ttaccgcttt tggaatgctt ttttccggat cgcgcgcttc agcggctgta atcccaatca
     4801 gctccagacc gccgaaggag aacataatta ccgccagcga caaaatcagc ccattccagc
     4861 cggtggcgaa gaaaccaccg tagcgccaga ggttgtcgat actggctttc tcgccgccgt
     4921 aaccagaaaa cagcagccac aggccaaagc cgatcatacc gatgatcgcc agcactttaa
     4981 tcagcgcaaa ccagaactcg gtttcgccat ataagcgcac gttcaccagg ttaacggcgt
     5041 tgatgataat aaagaaggcg gcagcccaaa tccacgttgg aacatccggg aaccagtact
     5101 gcatatagat gcccgcagcg gtcagctctg ccattcccac cagcacgaac attacccagt
     5161 agttccagcc agagaggaag cccgcaaacg gtccccagta tttataggca aagtgggcaa
     5221 atgaaccgga taccggctct tcgaccacca tctcgccaag ctggcgcata atcaggaaag
     5281 cgatgatccc ggcgacgccg tagcccagca atacagccgg acccgccatc tgaatcgccg
     5341 ggccaatgcc aagaaacaga ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat
     5401 gacggttatg taatccgcga tgaagcgtcg gctcttgatt cgacgcagta tcttccgata
     5461 cggttgacgc gtttttcacg cctttcccct gtgtgtcttt tttgttgagg ggcacctttt
     5521 aacatttagt gcccatcgtc gcaagacaca atccacacgg ttaaacgagg tatcctgctt
     5581 taattccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac
     5641 agcgacagca aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc
     5701 acctctgaac cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc
     5761 accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt gatacaacgc ctcatccagc
     5821 ttctgctcgc tgaatgtttg tggattattc aacgacggct cggcctctat ggcgtgacgt
     5881 aaatacatca gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaagccg
     5941 gtacccgtcg ccacagaaag atgaaagccc atccaccaca gtaaccagat gccgcccacc
     6001 agcgcaaacg gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga
     6061 tacaacagca cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg
     6121 gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc
     6181 actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg
     6241 gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg
     6301 gtagagactt taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg
     6361 atcggcaact ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata acgcagatta
     6421 attggataac gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa
     6481 gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gtgcggcttt ttcacggtta
     6541 atctcaacgt tgatatagcg cccaccttcc agccgctcag caagcgcaga agctacgcct
     6601 ggcaccgttc gcgccacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca
     6661 gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg
     6721 ttacgaattg gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt
     6781 tcctcaatga ttttgtccat tgtcatgcct ggccgccact gatcctgtgg cttaagctgg
     6841 atggtcgttt ctaccatctc cagcggagca gaatcggtgg cggtttccgc tttcccggtt
     6901 ttgccaaata cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc
     6961 atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg
     7021 ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc
     7081 agcaccgaaa gcgccgccac cagcagcgtg gttttcggcc agtgcagtac tttcagcaac
     7141 agcggatgat aaacacgaat caaaaagcga ttgagcggat tactgctttc cggcggaatt
     7201 ttgccacgga tccagtaacc catcaggata gggatcacta cgatcgccag cagcgccgca
     7261 cccgccatcg catacgtttt ggtgaacgcc aacgggccaa acagacgccc ttcctgccct
     7321 tccagggtga agatcgggat aaacgacaac gtgataatca gcagactgat aaacagcgcc
     7381 ggccccactt caacagacgc atcggtgatc acctgccagc gcgttttatt atccagcgtg
     7441 gcgtcaggat gctggtgctg ccactcttcc agctgtttat gcgcattctc gatcatgacg
     7501 atagcggcat cgaccatcgc cccgacggca atcgcaatgc cacccagcga cataatattg
     7561 gcattcagcc cctggaaggg catgacaata aaagcaatac acaaccccag cggcaacgaa
     7621 ataatcgcca ccagcgccga gcgcacatgc cagagaaacg gcgcgcagac taccgccacc
     7681 acaataaact cttccagcaa cttgccgctg aggttgtcga tggcccggtc aatgagctgg
     7741 ctgcggtcgt atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc
     7801 agtttgtcct tcacggcggc gatcacttct cgggcgtttt tgccggatcg caggatcacc
     7861 accccgcccg ccacttcgcc ttcgccgttt agttcggcaa tgccccggcg catcttcggg
     7921 ccaatctgga ccttcgcaac atcgcgcaga taaacgggca cgccattttc actggctttt
     7981 aaaacgatgt gattaaagtc gtcgagcgtt tgtagatagc cgctggcgcg caccatatat
     8041 tctgcttccg ccagttcgat tgacgaaccg cccgcttcct ggtttgaagc atccagcgcg
     8101 ctttttactt cggcgagact gatgccatac tgcgccaggc gctgaggatc gataaccacc
     8161 tgatactctt tcaccacacc gcctaccgac gccacttccg caacgtcagg aatggttttc
     8221 agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggcaagatc gtgcttaccg
     8281 ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc
     8341 tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc
     8401 gaacgcgccc agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca
     8461 aactgcgaga aaccacgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc
     8521 ggataagtca cctgattttc aacgatttgc ggtgcctggc cgggatagct ggttttaata
     8581 atcacctgca catcggagag atccggcaac gcatccactg gcgtattaat gatggtccag
     8641 gtgccccaga tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag
     8701 cgacgaataa tccattcaat cattggttat tccctcaatg cgcatgggta gcactttcag
     8761 agcgcatccg ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc
     8821 cggaaaccac cttttcacct tccgccagac cagagcgtaa tgcggtgacg ccttgcgatg
     8881 cctggaaaac agcaacgcgt ttcggtacaa agcgcccgtc ggcatcaacg gtaatcaccc
     8941 gctgttcgtt gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg
     9001 cggtgttgag ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga
     9061 cttccagacg cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt
     9121 tgcggatggt gagtgtttta tccggtcgcg ccggaacggt gagggtaaac tgcgaggcat
     9181 ctttcaccag ccaggcgatg gactccggga tcgcagcagt gacccacacc gggtccatac
     9241 cctgaattcg cgattacgcg caatccccct gagaaccgcc atgaacaagg gtttcatgat
     9301 ttgtgatttt agtttggtac gcaatttggt acacaacact attttcactt cagcgggtag
     9361 tcatcaaact caccataacg agcatcgttg atgatgtatg tgatcaccac aaatatcaca
     9421 tctggactag ttccgtcttc atgcgc
//
LOCUS       DAJTRM010000131         9438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRM010000131 DAJTRM010000000
VERSION     DAJTRM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.131"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            34..1149
                     /locus_tag="OO322_003599"
     CDS             34..1149
                     /locus_tag="OO322_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908331.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1828..2046
                     /locus_tag="OO322_003600"
     CDS             1828..2046
                     /locus_tag="OO322_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5908332.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2264..2506
                     /locus_tag="OO322_003601"
     CDS             2264..2506
                     /locus_tag="OO322_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908333.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2836..3765)
                     /gene="fdhE"
                     /locus_tag="OO322_003602"
     CDS             complement(2836..3765)
                     /gene="fdhE"
                     /locus_tag="OO322_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5908334.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3762..4397)
                     /gene="fdoI"
                     /locus_tag="OO322_003603"
     CDS             complement(3762..4397)
                     /gene="fdoI"
                     /locus_tag="OO322_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5908335.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4394..5296)
                     /gene="fdoH"
                     /locus_tag="OO322_003604"
     CDS             complement(4394..5296)
                     /gene="fdoH"
                     /locus_tag="OO322_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5908336.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5309..8359)
                     /gene="fdnG"
                     /locus_tag="OO322_003605"
     CDS             complement(5309..8359)
                     /gene="fdnG"
                     /locus_tag="OO322_003605"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7772..7774),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5908337.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8553..9386
                     /gene="fdhD"
                     /locus_tag="OO322_003606"
     CDS             8553..9386
                     /gene="fdhD"
                     /locus_tag="OO322_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5908338.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2310 a         2315 c         2446 g         2367 t
ORIGIN      
        1 gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac aaaaacagct
       61 gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc gtcgatcacc
      121 aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt caacgtatat
      181 catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc aattgtgtta
      241 tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat aataggaaat
      301 gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct tagtgcaatt
      361 aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac cgcaatgatg
      421 agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc ctgtgaagcg
      481 catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat ggcgacaggt
      541 gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca aagtctttct
      601 ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa ccagtcggat
      661 atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact ggtagataaa
      721 ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt agcgacggcg
      781 ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg taaatctgcg
      841 aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact ccttgaaaaa
      901 cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata ttttagttgc
      961 gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat tcgagttctt
     1021 catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga tgttttacaa
     1081 gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac cgtagggatg
     1141 ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca ttgcaaaaat
     1201 gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg aagccataca
     1261 gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg ctcaggagtt
     1321 ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta aaaaaactca
     1381 tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt tccggaagga
     1441 attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt aaagaccggt
     1501 tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat cctggtgctg
     1561 ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat atccctgaat
     1621 ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat ttttgagtaa
     1681 taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa aaacaaaatg
     1741 acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc acatacatta
     1801 atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct tcatttatca
     1861 gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc acgatctgca
     1921 attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt gcgtaaagca
     1981 aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa ggagcataag
     2041 gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt ctggtattaa
     2101 tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta tctggaaaac
     2161 gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc ggcaagggta
     2221 cttttctctc catagattca aaaatgatat tggcgagata tttatgaact ctctggcagg
     2281 tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc aacttgatga
     2341 tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg tagatcaata
     2401 cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc aagggtgtga
     2461 ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat
     2521 gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt gaacgcctta
     2581 tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg cctgataagc
     2641 gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc ttatcgatgt
     2701 gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg ggtaacgcgg
     2761 aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc tgcgccaccc
     2821 ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa tggaactacg
     2881 ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac
     2941 ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc cgcagtcatc
     3001 gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat gcagtttgcc
     3061 gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt cacacaggtt
     3121 gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg acaccggcat
     3181 agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg ctttgccggg
     3241 gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa acggcgcttt
     3301 atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat cttccagctc
     3361 ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc tcatttcagg
     3421 tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac gcggcagaac
     3481 gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg cggtcagatc
     3541 catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa gcgcagcaaa
     3601 gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct cggcgcggcg
     3661 gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg ccgtacgttt
     3721 ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc tttcttttcc
     3781 gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca ggcgctggta
     3841 acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc
     3901 accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg
     3961 atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac gccgctcacc
     4021 agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc gaagttataa
     4081 cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt cgcccaaaag
     4141 atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat gatgaacgag
     4201 gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg
     4261 atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac cgccgccagg
     4321 atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta gcgcacgatg
     4381 gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta ttctcttcct
     4441 catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca aaggtagccg
     4501 caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg gtttcgctga
     4561 tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg tgcagcacgt
     4621 acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg tcgtaaccgc
     4681 gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct ttcgtgccga
     4741 agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca accaccacgc
     4801 ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt
     4861 cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac tggaagtcga
     4921 cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc aggcagcctg
     4981 gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg tcgttctgct
     5041 ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg gggttgtcgt
     5101 acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa cacgccacct
     5161 gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact tcttcctgga
     5221 agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga atgatatctt
     5281 gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca aggaaggact
     5341 taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta ttggcaataa
     5401 agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg gtatcgatat
     5461 ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg gctttgatat
     5521 agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt ttattcgcca
     5581 gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac agcgcgtgtt
     5641 tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac gggaacttat
     5701 cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg ttcgagataa
     5761 cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag tgttccggga
     5821 acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc tgctgcatga
     5881 taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata tcccagccgg
     5941 tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg ttaccctgcg
     6001 gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc catgcccagc
     6061 ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc tggttgcctt
     6121 ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg tcatcgcgca
     6181 attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg gttgccggat
     6241 cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc gacttcggct
     6301 catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc gcgccaccct
     6361 gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca ccgtcagtca
     6421 gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga cctgagttaa
     6481 cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact tcggtctgga
     6541 tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta gaggtttcag
     6601 tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg atcactttgt
     6661 ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca ttgaccttgc
     6721 cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac ttcggcaacc
     6781 agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag
     6841 agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc agcggttttg
     6901 gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc agcgtcatgt
     6961 aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata ttggagtgac
     7021 cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc agctggatca
     7081 tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg agggcataca
     7141 ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag actttcagga
     7201 acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag cgggaaacgt
     7261 gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga
     7321 agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt ttttccgcgt
     7381 cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc aggctggcgt
     7441 tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg tacagcaata
     7501 cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag tcagctaccg
     7561 ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg tgaattttgg
     7621 cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg cccatcacca
     7681 cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg cgaccaaatg
     7741 ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg tcgaccgcga
     7801 gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg
     7861 aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg gcgttttgcg
     7921 caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca aacgcctctt
     7981 cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga aacttcagac
     8041 ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt gcaccgcggt
     8101 tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg ttttttgctc
     8161 cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg caggtattac
     8221 gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct acgctgggtg
     8281 caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc ttaaagaact
     8341 gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc ccatttacat
     8401 tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg gccgcaaagg
     8461 tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat accataatgt
     8521 tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga aaagaaatcg
     8581 aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt gatgatttac
     8641 aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc tacaacggca
     8701 tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg ctcggttttt
     8761 cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat gtcgttcctt
     8821 cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg gggttgaagg
     8881 agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg gagcaactta
     8941 atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat ctcaacaaac
     9001 tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg actggttgta
     9061 ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat gaagacgtgg
     9121 gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg gaaagctggc
     9181 agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa aagtcggcga
     9241 tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg
     9301 ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca acggtttata
     9361 cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata ttaaattgca
     9421 ggacttattc atttcgtg
//
LOCUS       DAJTRM010000132         9335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRM010000132 DAJTRM010000000
VERSION     DAJTRM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.132"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..406)
                     /locus_tag="OO322_003607"
     CDS             complement(<1..406)
                     /locus_tag="OO322_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5908339.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDAC"
     gene            complement(473..1198)
                     /gene="fimC"
                     /locus_tag="OO322_003608"
     CDS             complement(473..1198)
                     /gene="fimC"
                     /locus_tag="OO322_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5908340.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1235..1774)
                     /locus_tag="OO322_003609"
     CDS             complement(1235..1774)
                     /locus_tag="OO322_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5908341.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1839..2387)
                     /gene="fimA"
                     /locus_tag="OO322_003610"
     CDS             complement(1839..2387)
                     /gene="fimA"
                     /locus_tag="OO322_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5908342.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(2868..3464)
                     /gene="fimE"
                     /locus_tag="OO322_003611"
     CDS             complement(2868..3464)
                     /gene="fimE"
                     /locus_tag="OO322_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5908343.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(3942..4544)
                     /gene="fimB"
                     /locus_tag="OO322_003612"
                     /pseudo
     CDS             complement(3942..4544)
                     /gene="fimB"
                     /locus_tag="OO322_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            5998..6714
                     /gene="nanC"
                     /locus_tag="OO322_003613"
     CDS             5998..6714
                     /gene="nanC"
                     /locus_tag="OO322_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5908344.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6734..7840
                     /gene="nanM"
                     /locus_tag="OO322_003614"
     CDS             6734..7840
                     /gene="nanM"
                     /locus_tag="OO322_003614"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5908345.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            7905..8885
                     /locus_tag="OO322_003615"
     CDS             7905..8885
                     /locus_tag="OO322_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5908346.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2736 a         2031 c         1953 g         2615 t
ORIGIN      
        1 cacaggcatc atccgccagc agattcatac cggcgacaga agccgtattc agccccatac
       61 tggcgagttg cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg cccgtattaa
      121 atgtgacatc acgcgttgcc atataaccat tattcaaata gatatcgacg cgatacgtcc
      181 ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc ggccacagcc tggggatcat
      241 ccgctaaaaa gcgcagatta aaatagaggt cggcagatga caaaggtgcc tgtgcggcaa
      301 aagcacaggc gacaacgagt cggacaaaaa aaccagccaa acgatgctta cgaatatgca
      361 agcattgtgt gtttcgctgg taaagtctta aattcagata tgacattatt cctccggttt
      421 aaaatcaccc ggcagtcaat tctttttatt caggcgaaaa attccccctg cgttattcca
      481 ttacgcccgt cattttgggg gtaagtgcgc cataatcatt tattgttcgg taagtaatat
      541 tgcttcctgc atcagaaggc aatttaaccg tgctttcgcc cattggaggc accaatgcat
      601 tttcaagaac ccgggttccg gcattcaact ctgttaccgt caggtaatag ggtgtcgggt
      661 taatcagcgt cagagaattc gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg
      721 gtggcaacgc taatttagcc gggcgatagt acagtttaat gcggctgata attgcgagct
      781 gtagcatatt ctcagtcaat tttgatttat ccattgacgg aatcgcttta acgttcatcc
      841 agaataaact ttcccggtcc tgtggcaatt ggttatttgt tgcatcaaga atacgtaagg
      901 tattctcttt ttttcccttc atcgcaaaca gaggaggcgt cacgataaaa cgaccatcct
      961 ttacaccatc ggcattttcc acccatgatt gaattaaata ggtactattt tcatcattat
     1021 ttgtcacggc aagttgcact tgtttttgcc ctgccggata aattacgcga gtcgcaccta
     1081 aggccactcc tgcttcagcg cgtccggcaa ccatcattgc catgaacatc aggatacctg
     1141 ccagcaagca gaatgttatt tcctgcgatt tccttacatt gacgttttta ttactcactg
     1201 tcctgttcct gttatcacat tatctactga acaattactg ataggttaaa gagaaccagg
     1261 cctgggcatt ggcgatgccg ccagtaaccc gacgcccggt agcacgatat ttggcgatga
     1321 aatgtagcga agtagagcct gagtaaagcc gtttccagtt tgctggagga cgattaatcg
     1381 gtacgaggtt tccttcatca tcaaacaacg ctacaccaat attggaggct atccctggcc
     1441 cctctcccac ggagagcaca tccggatttt taccatccgc gacaccgtga aacgccacac
     1501 ccacacgttc actcaccacc gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg
     1561 cgctatcttc ccctgccgca tgaaaccggt tactgctgat ttgccccata ttgaccgtca
     1621 tttgtttatc accggcttca atccggcaag tttccgcaat aatgacgccc tgaaattgca
     1681 tatttccgcc gggcagcgtg gtattccatt tatttccggc cagagcaaac attggcagca
     1741 acgacgctaa cagaaataat ctcttccttt tcatcgttat ttttatcgca caagggtggg
     1801 catccctgcc cgtaatgacg tccttgaacc tgggtaggtt attgatactg aaccttgaag
     1861 gttgcatccg cattagctgc acccggggtt gcctcgccga ttgcataata acgcgcctgg
     1921 aacggaatgg tgttggtacc gttattcagg gttgtttgtg cactaaatgt cgcaccgtcc
     1981 agcgtcagag cattgcctgt tctgtctagg atctgcacgc caacgtttgt tgcactacct
     2041 gcagcagaac tctgcagagc cagtacatca gtacgcgtag catcaattgc cgtacctaag
     2101 aaggcaacag cggcttttgt ggcaacagtg gtatcgcaat cattcagctg aatgttaaaa
     2161 ccaacggcag agctggttgc tccagcctgc ttcaggctag cggtacgaac ctgtcccaac
     2221 tgaacggttt gatcaacaga gcctgcatca actgcgcaag cggcgttaac aacttcccct
     2281 ttaaagtgaa tggtcccacc atttaccgtc gtagtatcgg ccagagccgc tgcggaactg
     2341 agggacagag ccgacagaac aacgattgcc agagttttaa ttttcatgct gctttccttt
     2401 aaaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga
     2461 gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg ggccattttg
     2521 actcatagag gaaagcatcg cgcacaaact ttttcagttt atttgttggc ttaatattct
     2581 attattatct ttatttatag atttttatat tgcatgaggt ggtttttgga gagaagaatg
     2641 aggaagatgc gtcgagccac agaaacgtta gctttacata tagcggaggt gatgtgaaat
     2701 taatttacaa gagaaataat ttacatatca aatagttaga tgctttttgt cgttttttaa
     2761 tatttttatg cttgagaaaa aatacgtaac ttatttatga tatggacagt ttggccccaa
     2821 ttgtcttgtg tttatttgtt ttttaacttt attatcaata agttaaatca aacctcttct
     2881 ctttttaatt tttcgtttat gagattattt ctttcccata atccggcaaa acgagcagca
     2941 ttactggcgg tataacgcac agtatggcga atatttcgat gcccgagata atcctgaatt
     3001 aaacgggtat ctgcaccacg ctccgccagt tcataaccgc aagcatgcct taacatatga
     3061 ggatgagtct gcgttacggt tccagcttca ataccggcat cgcgaataat gcgataggcc
     3121 tgctggcgag aaagccgact cccgcggcga gaaataaata tagcgtcggt ccggtcagcg
     3181 cctttccagt tagcacgttc ctgggtccag cgttccacgg cttcacgctc atcaaaacgt
     3241 aacgggtgaa cggtagaaaa tccgttcttc agtcggcgaa tatttattcg accttcatta
     3301 aggtcaaggt cctgataatg cagatcaagc agttcactaa tacgcatccc atgccgatat
     3361 gccaacagaa taagacaata atctctggct cccgttgccc cgtaacaaac cgcctgcatc
     3421 atggcctgaa cttctttacc ggtaagataa cgacgtttac tcacaatagt agtactccta
     3481 actgagatat atttgaatga atacctatag gaaacctcaa tcggtcaaaa ttagcccaat
     3541 agataaaaag ataaccacac acgaagttat gtggctatca gtcaattaca ttaaacatca
     3601 tacaaaaata aaatatcact gatgtgacaa catctatttt cattgatttg attcaatata
     3661 acaatagatt acagaggatt aacttccagt tacttaaaaa ataaagaaaa aagtgtggaa
     3721 acaggagaat ttaagtgatg tgtaaactta tgaatgattt tgtttattta ttttgaagaa
     3781 cataaatcat tagattttgt atgggtatgg ttaacttaat gtttattatg ataattaaga
     3841 atcacactta caataacatc aattaataaa tggttaatca tttttgatat atcgtaagaa
     3901 taatgtagtt tttaacacca tccctggtat ctcaactatc tctataaaac agcatgacgc
     3961 tgtcgtcctc tggctctatc ccagatgccg taaaaacgcc ctgcattgct ggcggtatac
     4021 cagacagtat gacgaatatt gcgatgccca agataatcct agataagtcg cgtatctatt
     4081 cccatattcg ccaaagcaaa accacacgaa tagcgtaaca tgtgcggatg aatctccagt
     4141 gacaacccgg cattaccacc agaagtcgag ataatatggt aaaactgttg ccgagaaagc
     4201 ggattcccct tacgtgataa aaatacccac tcgctctcag catgcggata agaagtacgg
     4261 atactcaacc agttttttaa agcctgaact tctttgttca atagcgggtg cgttgttgaa
     4321 aagccttttt ttaatcgatg gatatatata cactttgctt taagatcaat atccgaaatc
     4381 ctcaatcgac aaatttcact cgcccggaaa ccatgaataa aacaaagcaa aatcagacaa
     4441 taattacgtg ttgcatgagg cccggtattt gctgctttaa ggagtgactc gatttcacta
     4501 tgggtaagga agttcctttt tttgttatca gccttattcc tcatcgtttt cccttataat
     4561 tacagacgcg cactagctgt gctgggttag caataatcca acatttttat cctgattatg
     4621 tttataaaag cgaacgtttg cttaataact tacgtaagtg gaccagttct tctgagcgaa
     4681 cttaaatgga gtagcgatag ttacttataa taactttgcc atacaaacgc catgctcttg
     4741 catgctatgt acctttatat atttatcaat ctgatacgaa tctaataata accctcatca
     4801 ttaatgcaac taatcttatc tatatatcat gtgatatgtt ctgtaacaag taaaatcaac
     4861 ataaaaaatt cagcaaaatt taatatttgt taaactagcc gtctcaaata tgacattatt
     4921 agttaactgc atttgccaca aaatggctat ttcatggaac gcagtaattt cttagtggct
     4981 attatcatgc taattcttag atccctagcg attattcctg cctggttcgt cagcttaggc
     5041 catgagattg ttacattcct ttttatcctg gagatatggg ggaacgcagg cgacgctttt
     5101 tcatctacat ccgttggtaa atatagcttt ggtcactacg acttaagcct tcctcaatca
     5161 cagctattcg ccgggcgata cggcaaacaa actaagtgaa acatcatcct gaaaacacca
     5221 acatcaacaa gcctctccag atcgacatca ggagtgacca gttacaagcc acaaaccaag
     5281 aaacatattt catgatgaga attatgctca gcctgatggc gagtaaaccc ttattttcac
     5341 cactctcact gagttagctt gcaaagcttt ctttgtttag attctaaata aattatttat
     5401 aaaacgtata gttaaaagaa aatctttagt ttttgaagac caccgcaagt gttcgtctgg
     5461 cttcacatgg catcttcttc ttagaaaaag atcgacatat tttgtgacgc gaattgcaaa
     5521 tctggttttg ttgtatggat tgcgtgattt ttgatctggt ataacaggta taaagatgca
     5581 ccacgatagt caatgagact gggcatctcg taatctatgg caaacatcac ttcagttctt
     5641 tcacatcggg tgatgaaaat gcacttcagt ctgaaaggaa tatgaaaatg agatcaacag
     5701 acactctatt ttatgactct gggtaaaatg ggttgagtaa gtgatataac ttacgaatat
     5761 tcaaatcaat taaaaatcag aagagatttt atactcaggt atttaatctg gatctctgtt
     5821 tatttaaata atgttaaaag agaattttca caggagacct tatacaaaaa atataaaata
     5881 cagttacccg ttgccaaaga cactataagc ctggcaaaaa aatattacac aacataaatg
     5941 ctaattgttt atgcgggctt tgtattgctt tctgtatcct acaaatgagt gaaatttatg
     6001 aaaaaggcta aaatactttc tggcgtatta ttactgtgct tttcgtcccc attaatttct
     6061 caggctgcga cactggacgt acgtggtgga tatcgtagtg gaagccacgc ctatgagact
     6121 cgactcaaag tcagtgaggg atggcaaaat ggatggtggg caagcatgga aagtaatacc
     6181 tggaatacca ttcatgataa taaaaaggaa aatgccgcac tcaatgatgt tcaggttgaa
     6241 gttaattacg caattaaact tgatgatcaa tggacggtgc gcccgggaat gttaacgcat
     6301 tttagcagca acggcacacg ctacggaccc tacgtaaaac tgtcctggga cgcgacaaaa
     6361 gatcttaatt ttggcattcg ctatcgttac gactggaaag cttaccgaca acaagactta
     6421 tccggtgata tgtctcgtga taacgttcat cgttgggatg gatatgtcac ttaccatatt
     6481 aatagtgatt tcaccttcgc atggcaaacg acgctataca gcaaacagaa tgattatcgc
     6541 tatgcaaacc acaagaaatg ggcgacggaa aatgcctttg ttctacaata ccatatgacg
     6601 cccgatatta cgccatacat agaatatgac taccttgacc gccagggtgt ttacaacggc
     6661 agagataatt tatcggaaaa cagttatcgc attggtgtgt catttaaact gtagtagaca
     6721 ggagacagtc acaatgaata aaactataat ggcgcttgct atcatgatgg cttcatttgc
     6781 cgcaaacgcg tctgtattac cggaaactcc tgtgccattt aaaagtggta ccggagcaat
     6841 tgataacgac actgtctaca ttggtttagg tagcgcaggt acagcatggt acaagctaga
     6901 tacacaggcc aaagataaaa aatggactgc gttagctgca ttccctggtg gaccgagaga
     6961 gcaagcaacc tctgcattta ttgatggcaa tctgtatgtg tttggcggca ttggcaaaaa
     7021 cagcgaggga ttgactcagg tatttaatga cgtacacaaa tacaacccca aaaccaatag
     7081 ctgggttaaa ttgatgtcgc acgcgccgat gggcatggcg ggtcatgtga cttttgtaca
     7141 caacggcaag gcttatgtta ctggcggtgt taaccagaat atcttcaatg gctattttga
     7201 agatctcaac gaggctggaa aagattcaac cgctatagat aaaatcaacg cccactattt
     7261 tgacaaaaaa gcagaagatt atttcttcaa taagtttctg ttgtcttttg atccctcaac
     7321 acagcaatgg agttacgctg gcgaatcgcc ctggtacgga acggctggtg cggcggttgt
     7381 gaataaaggt gataaaacct ggcttattaa tggcgaagcc aaaccaggat tgcgaacgga
     7441 tgccgtattt gaacttgatt tcaccggtaa taatttaaaa tggaataagc ttgatcccgt
     7501 ctcatcacca gatggcgtcg ctggcggttt tgcggggata agcaatgatt ctcttatatt
     7561 tgctggaggg gccggattca aaggttcacg agaaaattac cagaacggta agaactatgc
     7621 gcatgaaggc ctgaaaaaat catatagcac tgatattcat ctttggcata acgggaaatg
     7681 ggataaatcg ggtgaattat cgcaaggtcg ggcctacgga gtatcattgc cctggaataa
     7741 tagtttatta attattggcg gtgaaactgc aggcggcaaa gcggtgacgg attcagtttt
     7801 aatttctgtg aaggataata aagtcacagt acaaaactaa cgcttcaggg ccccggtaaa
     7861 ggggccctgc tatcaatttg tcatttgtat tacgcgaaaa ttatatgaac gcaataatat
     7921 cacccgatta ttactatgtt cttaccgttg ctggtcagtc taatgccatg gcgtatggcg
     7981 aaggactgcc attaccggac agggaagatg cgcctcattc cagaattaaa caattagcga
     8041 gatttgcgca tacgcatccc ggaggcccgc catgtcactt taacgacatt attccactga
     8101 ctcactgccc acacgatgtt caggatatgc agggttatca ccatcctctg gcaacgaatc
     8161 atcaaacaca gtacggcacc gttggccagg cactgcatat tgcacggaaa ttactgccct
     8221 tcattcctga taatgcaggg attcttatcg ttccgtgttg ccgtggcgga tcggctttta
     8281 ccgcgggcag cgaagggaca tattcagaac ggcacggagc cagccatgat gcttgtcgtt
     8341 ggggaacgga tactccgctg taccaggatt tagtcagcag aacgcgagcc gcactggcaa
     8401 aaaatccgca gaacaaattc ctcggcgtat gctggatgca aggcgaattt gacttaatga
     8461 ccagtgacta cgcgtcacac cctcaacact ttaatcatat ggttgaagcc tttcgtaggg
     8521 atctaaaaca ataccattct cagcttaata atattactga cgcgccgtgg ttttgcggcg
     8581 ataccacctg gtactggaaa gaaaatttcc cgcattcgta tgaagctatt tatggcaatt
     8641 atcaaaataa tgttttagcc aatattattt tcgtcgactt ccagcaacaa ggtgaaagag
     8701 gactgacgaa cgcgcctgat gaagacccgg acgatttaag cacgggatat tacggttcag
     8761 cgtaccggtc accggagaac tggacgacgg cactgcgaag cagtcatttc agcacggcag
     8821 cccgtcgggg gattatttct gacagatttg tagaagcaat tttgcagttt tggcgcgaaa
     8881 ggtgagcggg gagtttattc atgggggatg tccttgttcg ttctcagagt agacaacaaa
     8941 aaacaaatga gatcaaggta atatctcttc acttgcgggc tcgaacagca ggcaattgcc
     9001 aatataataa tctggcacaa gggtgaaaaa cgcgcaccgc ataaaccgtt gttattgctt
     9061 acagattagc cggatgcctt aatagacatc cgcgcctttt cgattatggc tcggaaatct
     9121 acgaacatct acacagttta ctggaacgtt ttgggccaca gcgttcgcag caccgtcctg
     9181 atatgccgtt ctggcgatta taagtcgacg gattctggca aatagacgat gatgatatgc
     9241 ttaatgatgt tcaaagaaat taggccatta ttatattatt gcttgcattt tctttcattc
     9301 ctaaaagcac ccgccattaa tcccaggcgc attat
//
LOCUS       DAJTRM010000133         9281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRM010000133 DAJTRM010000000
VERSION     DAJTRM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.133"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..246
                     /gene="mliC"
                     /locus_tag="OO322_003616"
     CDS             <1..246
                     /gene="mliC"
                     /locus_tag="OO322_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5908347.1"
                     /translation="QTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLSASG
                     ARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            305..961
                     /gene="pdxH"
                     /locus_tag="OO322_003617"
     CDS             305..961
                     /gene="pdxH"
                     /locus_tag="OO322_003617"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5908348.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1090..2364
                     /gene="tyrS"
                     /locus_tag="OO322_003618"
     CDS             1090..2364
                     /gene="tyrS"
                     /locus_tag="OO322_003618"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5908349.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2423..3286
                     /gene="pdxY"
                     /locus_tag="OO322_003619"
     CDS             2423..3286
                     /gene="pdxY"
                     /locus_tag="OO322_003619"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5908350.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3330..3935)
                     /gene="gstA"
                     /locus_tag="OO322_003620"
     CDS             complement(3330..3935)
                     /gene="gstA"
                     /locus_tag="OO322_003620"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5908351.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4041..5543)
                     /gene="dtpA"
                     /locus_tag="OO322_003621"
     CDS             complement(4041..5543)
                     /gene="dtpA"
                     /locus_tag="OO322_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5908352.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6154..6789)
                     /gene="nth"
                     /locus_tag="OO322_003622"
     CDS             complement(6154..6789)
                     /gene="nth"
                     /locus_tag="OO322_003622"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5908353.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6789..7484)
                     /gene="rsxE"
                     /locus_tag="OO322_003623"
     CDS             complement(6789..7484)
                     /gene="rsxE"
                     /locus_tag="OO322_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5908354.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7488..8108)
                     /gene="rsxG"
                     /locus_tag="OO322_003624"
     CDS             complement(7488..8108)
                     /gene="rsxG"
                     /locus_tag="OO322_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5908355.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8112..9170)
                     /gene="rsxD"
                     /locus_tag="OO322_003625"
     CDS             complement(8112..9170)
                     /gene="rsxD"
                     /locus_tag="OO322_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5908356.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2381 a         2325 c         2353 g         2222 t
ORIGIN      
        1 caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
       61 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcctt
      121 tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa aggtgaggaa
      181 gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca aaatccacag
      241 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
      301 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
      361 aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg aacgttggct
      421 ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg ctaccgtgga
      481 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
      541 ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata atccgcgcgt
      601 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
      661 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
      721 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
      781 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
      841 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
      901 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
      961 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
     1021 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
     1081 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
     1141 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
     1201 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
     1261 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
     1321 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
     1381 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
     1441 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
     1501 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
     1561 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
     1621 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct gcaaattggt
     1681 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
     1741 aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac caaatttggt
     1801 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
     1861 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
     1921 atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg taaagcaccg
     1981 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
     2041 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
     2101 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
     2161 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
     2221 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
     2281 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
     2341 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
     2401 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
     2461 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
     2521 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
     2581 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
     2641 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
     2701 cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt tttgcgatcc
     2761 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
     2821 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
     2881 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattga
     2941 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
     3001 ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc cactggtgga
     3061 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
     3121 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtctacga
     3181 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
     3241 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
     3301 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
     3361 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
     3421 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
     3481 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
     3541 ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc tgggcgaaac
     3601 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
     3661 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
     3721 tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggc
     3781 acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
     3841 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
     3901 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
     3961 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
     4021 taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc tgcagcgtca
     4081 tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc aataacggca
     4141 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcgggtcg
     4201 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
     4261 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
     4321 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
     4381 cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt cgcacccagc
     4441 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
     4501 ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
     4561 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
     4621 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
     4681 aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
     4741 ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
     4801 gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat cagcgccaca
     4861 acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc tggttttgaa
     4921 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
     4981 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggggtggct
     5041 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
     5101 agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
     5161 aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc gtcgtgacca
     5221 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
     5281 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
     5341 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
     5401 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
     5461 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
     5521 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
     5581 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
     5641 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
     5701 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
     5761 aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg cggcgatatg
     5821 ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta
     5881 atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa cctgattctt
     5941 gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
     6001 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata
     6061 acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt
     6121 tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct ctttgtattc
     6181 acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc aggtataacg
     6241 cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa ccactttcag
     6301 tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac
     6361 gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac
     6421 gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac
     6481 ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt ctgctttgct
     6541 gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta gttcaagcat
     6601 cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgatatc
     6661 ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag
     6721 ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca gacgttttgc
     6781 tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc agctgcttca
     6841 gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccaag
     6901 ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt gtggaaaatc
     6961 tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaacgtg
     7021 ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccgta
     7081 ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca
     7141 gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga
     7201 tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg
     7261 atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg
     7321 gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg
     7381 gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag tgcagagttg
     7441 tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag
     7501 gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc
     7561 gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca
     7621 tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc cgcaaaatgg
     7681 gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt ttcgtggtgc
     7741 tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag cagctgaatc
     7801 gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc
     7861 tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt taccagatag
     7921 caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc aaataacgtc
     7981 ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt gatggccgca
     8041 gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt tcggatagtt
     8101 ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc
     8161 agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg
     8221 aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag ccgaccacga
     8281 ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag catagtcgca
     8341 ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac
     8401 gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc
     8461 tgccatagca accacacgcc accagccagc caggcgagat ttacccattg ccaaccagcg
     8521 cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga atgacctgcg
     8581 cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag
     8641 cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac ctgtatggcg
     8701 tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc
     8761 accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgc
     8821 cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc cagtacgacc
     8881 atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt cagcaatgct
     8941 gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg
     9001 gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca accaaaaaac
     9061 cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat
     9121 gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg
     9181 tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc cgcggcaact
     9241 gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTRM010000134         9243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRM010000134 DAJTRM010000000
VERSION     DAJTRM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.134"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO322_003626"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO322_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5908357.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO322_003627"
     CDS             1828..3147
                     /locus_tag="OO322_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5908358.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO322_003628"
     CDS             3445..3486
                     /locus_tag="OO322_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908359.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO322_003629"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO322_003629"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5908360.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO322_003630"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO322_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO322_003631"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO322_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO322_003632"
     CDS             5815..6711
                     /locus_tag="OO322_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5908361.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO322_003633"
     CDS             6708..7682
                     /locus_tag="OO322_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5908362.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO322_003634"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO322_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO322_003635"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO322_003635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5908363.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2260 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 aga
//
LOCUS       DAJTRM010000135         9231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRM010000135 DAJTRM010000000
VERSION     DAJTRM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.135"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..421)
                     /gene="xdhC"
                     /locus_tag="OO322_003636"
     CDS             complement(<1..421)
                     /gene="xdhC"
                     /locus_tag="OO322_003636"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5908364.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLC"
     gene            complement(418..1296)
                     /gene="xdhB"
                     /locus_tag="OO322_003637"
     CDS             complement(418..1296)
                     /gene="xdhB"
                     /locus_tag="OO322_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5908365.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1307..3604)
                     /gene="xdhA"
                     /locus_tag="OO322_003638"
                     /pseudo
     CDS             complement(1307..3604)
                     /gene="xdhA"
                     /locus_tag="OO322_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4019..4774
                     /locus_tag="OO322_003639"
     CDS             4019..4774
                     /locus_tag="OO322_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5908366.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4853..4926
                     /locus_tag="OO322_003640"
     tRNA            4853..4926
                     /locus_tag="OO322_003640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4885..4887,aa:Gly,seq:ccc)
     gene            5061..6671
                     /locus_tag="OO322_003641"
     CDS             5061..6671
                     /locus_tag="OO322_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5908367.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6703..7310
                     /locus_tag="OO322_003642"
                     /pseudo
     CDS             6703..7310
                     /locus_tag="OO322_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8241..8987
                     /locus_tag="OO322_003643"
     CDS             8241..8987
                     /locus_tag="OO322_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5908368.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2353 a         2275 c         2365 g         2238 t
ORIGIN      
        1 gacaaagatt tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg
       61 gtttcgccag cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca
      121 ctgtgccgga tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac
      181 cttccagcgt gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta
      241 ttgctgtgcc gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt
      301 tgacacttag cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt
      361 gaagctgaaa aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca
      421 ttgcaatttt ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg
      481 gatgagatgc agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg
      541 caggacagat tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt
      601 ctgttcggca tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa
      661 ttcgctgaaa ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc
      721 cattgcgtcg cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg
      781 cggaaaatga aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt
      841 gtgaaagcca ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc
      901 gtcataaatt agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc
      961 accgtaggta gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa
     1021 cgccgggaga tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga
     1081 gccgatacgt agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg
     1141 aatatcaaca atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt
     1201 gccaccggcg agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag
     1261 ggttgctgcg cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg
     1321 ccagatggaa ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac
     1381 cggtagccat cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac
     1441 ccagtgactt atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca
     1501 gttgtggcag atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca
     1561 cgccgctttt agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca
     1621 ttccgccgtg tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa
     1681 cgttgaggat gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa
     1741 aggtacagcc aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct
     1801 gcccgccgcg ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg
     1861 gctcttccgg tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg
     1921 actgatgtag catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac
     1981 tgcgcagcgc aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg
     2041 gcgtaacgtc ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg
     2101 tttctgccac catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc
     2161 tttgcacgtt gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc
     2221 cgacaggcca ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat
     2281 tgccttgctg gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt
     2341 tttcaagaca ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat
     2401 ctccttcgcg ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg
     2461 cggcgtcatc aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca
     2521 tcgcaccagc cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac
     2581 gaggataaag gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg
     2641 cataagcgcc ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt
     2701 tcacgcccat ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact
     2761 cttcacggct aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg
     2821 ccattggctc ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga
     2881 tgactcgtac gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt
     2941 gcgggatctg ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg
     3001 atgttacgct ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct
     3061 ggtagtcggc ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca
     3121 gtaaattgcc gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg
     3181 tgataacggg taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga
     3241 gttcatcgcg ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta
     3301 gcagtgcgcg atcggcggta tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg
     3361 tagcgaatgg aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac
     3421 ttctggcttg ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat
     3481 atttcgcgta acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga
     3541 ccttagcaat ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt
     3601 ccatagaaat cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag
     3661 gcaagattta cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt
     3721 aaaaaaacac agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat
     3781 atgagtggcg ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata
     3841 gggtaaaaag tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc
     3901 tttgccaaat gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg
     3961 ggtatcaaga ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt
     4021 gagtgcggga cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact
     4081 gctattggcg ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt
     4141 ttacaccgtg aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt
     4201 aaaagagctg gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa
     4261 actaaaactg ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac
     4321 gactaaaacc gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt
     4381 agggcaacgt tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc
     4441 agatggcggc aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc
     4501 gggtgcagga aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat
     4561 gattaaacac agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa
     4621 taatgggcaa agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc
     4681 atctgttcgc ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta
     4741 cttgccgccg cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc
     4801 gcggcacttg ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt
     4861 agttcaatgg tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc
     4921 gctccatgac acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg
     4981 ccttccgata gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag
     5041 cagacctaag gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg
     5101 tcttttacat ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc
     5161 acaaccgtta taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg
     5221 ctcactcacg gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct
     5281 cttatgagga gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc
     5341 gtgatgacct gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta
     5401 agtccaacct tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata
     5461 ttaatgacgt tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg
     5521 gcctattgtc ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg
     5581 tattggcgga acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc
     5641 agtacgagca ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt
     5701 cacactctac gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga
     5761 cccgtgcaga tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc
     5821 aacgctttaa gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc
     5881 tggacgtcgt taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg
     5941 cggtacgcaa cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc
     6001 agcgtaaagc ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg
     6061 tcgcagctaa agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg
     6121 ctctggctgc gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg
     6181 tcaggaccac tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc
     6241 cgggtaagag tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt
     6301 atggttttgt attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg
     6361 ataatgctat ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta
     6421 gcaagtggtt taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt
     6481 ttcactcgtt tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt
     6541 atgctcaggc gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag
     6601 aggtcgaggt ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag
     6661 acggaaagta atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta
     6721 gttagcaaag aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg
     6781 ctggctggaa tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata
     6841 tttactgctt ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc
     6901 tacgataaga cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc
     6961 ggtataaaca attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta
     7021 agagacgcta ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat
     7081 ttggataacc aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg
     7141 gattaactgt tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga
     7201 tttctgataa tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc
     7261 tgatgttagc ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac
     7321 acctcactat agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc
     7381 accttaatag cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg
     7441 accgattgga aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc
     7501 ttttaacgta agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt
     7561 aagttggctc tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag
     7621 gtctgccatc catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag
     7681 gttggagctt tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg
     7741 attgtgttat cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc
     7801 cgaagtaaaa ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac
     7861 tgaacacttt cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata
     7921 gggaccatat ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc
     7981 tagcaagcta gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg
     8041 tctcgtgact taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta
     8101 tggtcatcgc agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc
     8161 tgctggtctc cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca
     8221 aacaaaagga gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc
     8281 ttgtgtccat gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt
     8341 tacgagatat tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa
     8401 ctttgagcag cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg
     8461 acgcgaaagg tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg
     8521 tcgctggcta ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc
     8581 gtcgagtcct tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc
     8641 gaggcgtgac ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga
     8701 ccgcacttct ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg
     8761 taaatccgaa gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag
     8821 caggtgggta taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga
     8881 tggttgacaa gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag
     8941 agcacctcct gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac
     9001 ccgcgcacac ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc
     9061 ctcagagagc ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag
     9121 ctaatagccc aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca
     9181 atacctaatt taaacacctt ttgagatgac cgatgcgggg agacccgtag g
//
LOCUS       DAJTRM010000136         9152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRM010000136 DAJTRM010000000
VERSION     DAJTRM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.136"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            369..1064
                     /gene="minC"
                     /locus_tag="OO322_003644"
     CDS             369..1064
                     /gene="minC"
                     /locus_tag="OO322_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5908369.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1088..1900
                     /gene="minD"
                     /locus_tag="OO322_003645"
     CDS             1088..1900
                     /gene="minD"
                     /locus_tag="OO322_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5908370.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1904..2170
                     /gene="minE"
                     /locus_tag="OO322_003646"
     CDS             1904..2170
                     /gene="minE"
                     /locus_tag="OO322_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5908371.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2542..2886)
                     /locus_tag="OO322_003647"
                     /pseudo
     CDS             complement(2542..2886)
                     /locus_tag="OO322_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2921..3040)
                     /locus_tag="OO322_003648"
     CDS             complement(2921..3040)
                     /locus_tag="OO322_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5908372.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3001..3186)
                     /locus_tag="OO322_003649"
     CDS             complement(3001..3186)
                     /locus_tag="OO322_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908373.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3287..3460
                     /locus_tag="OO322_003650"
     CDS             3287..3460
                     /locus_tag="OO322_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908374.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3462..3806
                     /locus_tag="OO322_003651"
     CDS             3462..3806
                     /locus_tag="OO322_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5908375.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3810..4145
                     /locus_tag="OO322_003652"
     CDS             3810..4145
                     /locus_tag="OO322_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5908376.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4202..6523)
                     /locus_tag="OO322_003653"
     CDS             complement(4202..6523)
                     /locus_tag="OO322_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5908377.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6640..6849)
                     /locus_tag="OO322_003654"
     CDS             complement(6640..6849)
                     /locus_tag="OO322_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908378.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7600..9123)
                     /locus_tag="OO322_003655"
     CDS             complement(7600..9123)
                     /locus_tag="OO322_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5908379.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2561 a         2116 c         2012 g         2463 t
ORIGIN      
        1 attaagataa cgggtatctg ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc
       61 ctggcgtggt gaaaaaatac acttgcctca atactggata tttaaacagg tctggagatg
      121 ctgtcacgat tagggtgcgg aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg
      181 caatgggttg attgacaagg gtatttttta agctatgaat cagcgccatt tatcacagaa
      241 tagactttta ctctgaataa atgggagggt gacttgcctc aatataatcc agactataac
      301 atgccttata gtcttcggaa catcatcgcg cgctggcgat gattaatagc taattgagta
      361 aggccaggat gtcaaacact ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg
      421 ttcatctgca tgaggcagaa cctaaggtta tccatcaggc gctgaaagac aaaatcgctc
      481 aggcccccac atttttaaaa catgcccccg ttgtactcaa cgtcagtgca ctggaagacc
      541 cggtaaactg gtcagcgatg cataaggcgg tttcggcaac cggtttgcgg gttattggcg
      601 taagtggctg caaagatgcg caacttaaag ccgaaattga aaaaatgggg ctgcctatcc
      661 tgacggaagg aaaagaaaaa gcgccacgtc cagctcccgc accgcaggct ccagcgcaaa
      721 atacaatgcc ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta
      781 tttatgctcc acaatgtgat ctgattgtta caagccacgt tagcgctggg gccgaattga
      841 ttgccgatgg gaacattcat gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa
      901 gtggtgaccg ggaaacgcaa atattttgta cgaacctgat ggcggaattg gtgtccatcg
      961 caggtgaata ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac
     1021 tgcagttagt cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg
     1081 aatttctatg gcacgcatta ttgttgttac ttcgggtaaa gggggtgttg gtaagacaac
     1141 ctccagcgcg gccatcgcca ctggtttggc ccagaaggga aagaaaactg tcgtgataga
     1201 ttttgatatc ggcctgcgta atctcgacct gattatgggt tgcgaacgcc gggtcgttta
     1261 cgatttcgtc aacgtcattc agggcgatgc aacgctaaat caggcgttaa ttaaagataa
     1321 gcgtactgaa aatctctata ttcttccggc atcgcaaaca cgcgataaag atgccctcac
     1381 ccgtgaaggg gtcgccaaag ttcttgatga tctgaaagcg atggattttg aatttatcgt
     1441 ttgtgactcc ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga
     1501 agccattatt accaccaacc cggaagtctc ctcagtacgc gactctgacc gtattttagg
     1561 cattctggcg tcaaaatcac gccgcgcaga aaatggcgaa gagcctatta aagagcacct
     1621 gctgttaacg cgctataacc caggccgcgt aagcagaggt gacatgctga gcatggaaga
     1681 tgtgctggag atcctgcgca tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt
     1741 gcgcgcctct aaccagggtg aaccggtcat tctcgacatt aacgccgatg cgggtaaagc
     1801 ctacgcagat accgtagaac gtctgttggg agaagaacgt cctttccgct tcattgaaga
     1861 agagaagaaa ggcttcctca aacgcttgtt cggaggataa gttatggcat tactcgattt
     1921 ctttctctcg cggaagaaga acacagccaa cattgcaaaa gaacggctgc agattattgt
     1981 tgctgaacgc cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc gtaaagatat
     2041 tcttgaggtc atttgtaaat acgtacaaat tgatcctgag atggtaaccg tacagcttga
     2101 gcaaaaagat ggcgatattt ctattcttga gctgaacgtg accttaccgg aagcagaaga
     2161 gctgaaataa gcccgctgta aaaacgcatt tatcttcaag gcagagtcat ttctgccttg
     2221 agtttttcat ccctctcatc cacgttgtgg taaagcggcg agtattcttg ctgatactcc
     2281 tcattgctat ttccacctcc ccctttacac cttaaggctg ttcccacgca cagaacaaaa
     2341 tgtttatgcc tggttgagta aataaccatg ttattagtgt gtatatactg ggagatgata
     2401 accccgcaat ttcattattc attgttctcc cataggatga ggtttcccgc cgtaactggc
     2461 gggctttttt tgccccagaa ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa
     2521 gcgcagttta atcgttgatg gtcagaacga ccagttcaca ccagccaccg cgttccacgg
     2581 ggactccaca ccggcaccat ggctataccc caccccgaaa tgcccgctta acgtactgct
     2641 gaatgaggct ttaatacctg cctggtatat tccacgtctg cccgacaaat cattgacaaa
     2701 attaccgtca ctattcactt tcacccgatt atcatcgaca aattctttgc gcacagccgc
     2761 cttcaggcag ggttcaattt ccataccgtt ccccagacgc atgttgtaac tcagcgttgc
     2821 acccagttca cgatatatac tgcgggtatc gactgatttc gattccatgc cattggataa
     2881 atgatagtag cgtctaagtg acctaagctc tgctgcaaca tcagcccgca ggcaccagac
     2941 caggggattc atcctgaaga gacagcgcag gtgtattgtg ttcaccgctc accaaagaca
     3001 tcatgatgaa atgatgatat cccgcataag aaagaggcat tttttacacg cagtgcgctg
     3061 aagtgtagtt ggataaaaaa tcactctttt cagataatac tgtcgtacgc ttatctttag
     3121 acaatgaggc cgtcggaaaa ataaaatgac taatgtaatc gttgctgatg aatatcttct
     3181 gctcacataa aaatcacaca ataatattga taaatcagat tatcttacct ttacaacatt
     3241 gcgttaacag tcaatgacac ttcttgaata aaaatattgt caatcaatga gttattcctc
     3301 atttaagatt attctcattc acgtaaaaaa cataattcct atcatcacag ccacgctcat
     3361 tttgaattat ctgaataata gcgagcgttc tttagttaag cagatcttaa ttgaagacga
     3421 aattattgtg tgtgcaactt acttaatacc ggacatctaa gttgaagaaa aaaatacttg
     3481 ccttcgggct tatctcagca ttgttctgct ccacgccagc gatagcggat atgaaccgca
     3541 ccacgaaagg tgcattgcta ggcgcaggcg ttggtttact tactggcaat ggtgttaatg
     3601 gcgtactgaa aggtgcagct gttggcgctg gtgttagtgc agtaacagaa aaaggccgcg
     3661 acggtaaaaa tgcgcgtaaa ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg
     3721 ttctgaccgg caatggcctc gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg
     3781 gtgcaattct gggtaaaatg aaataaggaa tgatgatgaa aaaatttgct ttactggcgg
     3841 gcctgtttgt tttcgcacca atgacctggg cgcaggacta caatattaaa aacggtttgc
     3901 cgtcagaaac ctatattact tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac
     3961 aggttgcaga aattgttgcg gtaatgggta atgccagcgt tgccagccgt gatttaaaaa
     4021 ttgagcaatc tccggaactg agcgccaaag tggtagaaaa actgaatcag gtttgcgcta
     4081 aagacccgca aatgctgttg atcacggcaa ttgatgacac catgcgtgct atcggcaaaa
     4141 aataatattt catatgtacc gcgtcgatga atacgacgcg gttttttagc taacggcggg
     4201 atcagaactt atatttcagg cccaccatca ctgcggtgtc attgtagcca ttatcaccca
     4261 gctgcacgcc cacattcccc cagacgctgg ccgctggatt caggttgcca tttacgccaa
     4321 gtttaatttc ccccaaatta ctcaccccat cttgttcgac tttcacaccg ttcattgaga
     4381 cagcaaaatc tttactgtta tggatccagt tcatttcaac aaaaggttca aagtcgtcac
     4441 cgtgcggacc gctgtttttc tcctgagtac gaataaaggt gcggaagccg agtcgggtct
     4501 ggatgttgtt atttgcgtca ttctcaatac gtgagccatt ggcttccgtg tgcgcattat
     4561 gatcgacgcc catccagata gcctgtgcct gtggctggaa gatccacgta tatttcgccg
     4621 cttccagacc aaaggattta ttcagggcaa aaccgtaacc cgcttccagc gagacggttg
     4681 cacctttcgc gctataggat tcataggcta attcatcccc tttcacggag tttttaaacc
     4741 agctatattg cgcccaggag tcaatgtatg cgcctttttt actgatgtca tcggcaaacc
     4801 aagtggcata cagccctgcg ctatagcctc tgacactccc tttcgaacga taatccgaca
     4861 cgctggaatg agttaagttg tagtcgcggg cataaccagc catcactccc aaacgccaac
     4921 tgtcactatc ggtaaaacca ccggttaaca gatcgccccc taactgcgag acgtagcgat
     4981 gcgaggtcgt tctcaactgt ccgttgctgt cacgccaggc attatgcccg ccaatttgac
     5041 gtagccaaag tcggcttgaa cgctcctgtt cagttacagg atcgatgtag cgcgtttcac
     5101 ccgcacggtc gttcagacgc ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt
     5161 aacttccagc ttcggggcgc aggacggggg taggcgcaac agtaggcgct gaacttcctc
     5221 cggagtttgt ttcaccgccg ctcgttgcct ggaagttagt cagataccag tttttattgg
     5281 tcgcttcggt attacctcgg gttaatgaat attcgtaggc accggcgaaa atcctcgaat
     5341 ccttaataaa ttccccattt gattccccct caacgctgat aatctctatc ccatttaacg
     5401 tgtaagctcc gctgcctcct gcattggtaa ctttaactcg ggttgttcca gaggtatttc
     5461 cattaataac gactttatcc gtagctgaat tatcatcatc caattcagtt ctgagatgca
     5521 atacgccatt attaccaacg taattttcag ttattgttaa tcttgttggt tcgacatccc
     5581 gtccatctgc ccgggaaata tagaccgtac tattatcaac cgtgaggttg ctcaccgttg
     5641 agtcagcttt aatatcccag gtactattat ctgaaagcga cagataggta tgagtgttat
     5701 catctgttga aatattagct gaacccgtta tttttgaatt agttgcatcg acggtcaaat
     5761 gacctttaat attattgata aaaacatctg cagccgatga tacctcacta tccgtaatgg
     5821 tcacgttact cgtctcatct ttatggtaga ggatttcatt tgctgaccgt agtgagctac
     5881 cagataaaat atctaccgtt gcttcctgac catttatgga agactccagc acaattccag
     5941 acccctgatg cgcgactgca gatgtatttt tcagcgtcac cgtgccgcca ttataaattt
     6001 tagcaatcgc ggcgtcagga cccgttgtat aaatttctcc ctggttaaat gaaactgtcc
     6061 cctggcgggc agcgatggca tgagcattgt ccccatgcgt ggtgatatca acatcggtta
     6121 atgtaacgct ggcattggta taatcaagcg tgtcaccagc cttgacgaca gtgccattag
     6181 cgtacgcggc ataactttct gttccgtggg ttgtgatctc cccttttgcc atatccagtt
     6241 ttgctatggc attattttca ttacttcctg tatcggcagt actttcttca tcaagagaag
     6301 atacacacgc acacgcgctt ccgggagatg cacacgcaag aacgccgact gcgtgattac
     6361 ccgcagtaac aataaatgta tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag
     6421 agttgactct tattatggtg tcagtattca ggtttccact cgtttgactt agcaaaccaa
     6481 ttgagtaatc ccccgttgtg acaatcgtgt ttttaccctg caaaataaca gcaccgccaa
     6541 attcacttac gattgcgcta gctgttaatg ctgggcttag aggactattg tcagaaggat
     6601 ataaaccgcc cgttgttaca atgacgccat tgttaatggt taacttacta ccctctcctg
     6661 caacggatac ccgtaacttc cattacccgt tgttataact agcgcattat caatatcttt
     6721 tacttttcca tcttttattg ccacagcatg tttatattca tcgtgctgaa taaactgttc
     6781 agcatatgca ttacccaccg ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt
     6841 cagtttcata gattaactat ccattcattg gattaacaaa cattcctggt caaataacct
     6901 atgagctcag attaccaaaa cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa
     6961 tagataaaca gcgacaatat ataaatgata aataagtcgg cattatcatt tttcacctta
     7021 ttttcatgac attgatcact ttggggtgac tattttgttt aacaaataac tcttcgacat
     7081 caaaattatt aatagttgcc atggtaatta tttcggtgat atataaaaac aactgtgata
     7141 acaccaaggt tgacaactat ttgcgcaata attcctttcc ttcaatgatc caggcgttca
     7201 ctgccagcaa cccattcagc cgcatactga cagtgagaaa ctgaaagatt attgctgatc
     7261 cctgtggcta acgagtcttt ctcttaaatc gattaaccag attgatcgcc agccaaacaa
     7321 gggcattaag caaatttatc gtcacaagca cttctcccag cccggtactg ggttttgcaa
     7381 taaggatgac caggagggtg atcagcataa atgcaatgga aaagatgata tagagagtga
     7441 aataatccag attattacta ttattcatgc tactaatggc aatcctgtca aaactttcaa
     7501 acaattatag caaacaaaat aatgaacaat agtaacaaga ggcaacgagc taattagctg
     7561 tctataatta caccgcacta tggccttact gatcaatttt cactcaacca caaccttcac
     7621 cttcggttta gaaaggataa ttttgaccag gtcgatgtat gtaacaggtt tatagaaata
     7681 ataaccctgc tgaaatgtga tattattttg gttcagatag ccaagttgtt ctttcgtttc
     7741 gacaccttca gcgacgatac tgatcgaaag tttacgcgcc agatccaata cacaatccag
     7801 aattcgggtt gattctgggt ctgcgtttac gcggccaacg aaactgtgat caattttgat
     7861 ataatcaata tgcaggtcat gaagataaga aagccctgag tagccagtac cgaaatcatc
     7921 cagcgcgatg acaaaaccat tttcatgcag aatgttcaac cgctgaacca gactttcatc
     7981 aacattcaat ggctcacgct cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa
     8041 actgtcacgg aaatttaaac actcgtcgac aaacgtcggc gaaataatat gcgaagcgct
     8101 aaaattaatt ccaatatgaa aaccttccgg cagtttactc gcgatagcgt tcatctgtct
     8161 ggcaacctga ttcatcaggc tttgtgtaag cggaacgatt aatccggatt tttcagcaag
     8221 tggaataaat gccgcgggtg aaatatatcc accgtgaggt tgtttccagc gggctaacac
     8281 ctcaactccc cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa aagggacgat
     8341 ctccccatta tttattgccc gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata
     8401 tctatcaagc aggaaggcag cggcgctggc gattaacaga ataaatatta aaatcccaaa
     8461 gcctttatcg ataagtctgt agaagctaaa gagtggcggc gggttaaaga taatagtgaa
     8521 atgatatttt gtggagtgaa ccttctcggt atagggataa tgtccattaa gcgtcatcac
     8581 atcagccgtt ggtccaataa tgtcatccgc cacgcgtaat acatacctta cccctttcaa
     8641 gggtacatta agcgccccgc gaatatgttg atcacttatc gtaaccaaaa tgcgcgtatc
     8701 tgcaaattgg ctctgataga gcaggatagg taatctatta acggtgtcga ttgccggagc
     8761 caacagtaaa ttactgtccg ggacaacagg aatacgagac agcaggaccc gattcccagg
     8821 cagggatgta caccagacta ttccctgttt gagaatgatg attgtgcgca ggtgaggttt
     8881 cagagctgct tcagtgccaa gctgatattg ctcctcgagg tcgcattcct tcccggcaat
     8941 gttaaccgct gtttgagtcg cctgtgacgc ttcttcaagt atatgattga tattgttggc
     9001 ggcatatctt gctcccgcca ggtactctgc ttttgcggaa taccagagct ggatgttcaa
     9061 aatagcgaca ccggtaataa acaagcatat cgcaacgagg atgggtatga gtgtattgcg
     9121 catttgcttg tcccggccaa agggtttaat ac
//
LOCUS       DAJTRM010000137         9137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRM010000137 DAJTRM010000000
VERSION     DAJTRM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.137"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(16..204)
                     /locus_tag="OO322_003656"
     CDS             complement(16..204)
                     /locus_tag="OO322_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5908380.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OO322_003657"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OO322_003657"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5908381.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="OO322_003658"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="OO322_003658"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5908382.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="OO322_003659"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="OO322_003659"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5908383.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="OO322_003660"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="OO322_003660"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5908384.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="OO322_003661"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="OO322_003661"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5908385.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5099..6231
                     /locus_tag="OO322_003662"
                     /pseudo
     CDS             5099..6231
                     /locus_tag="OO322_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6320..6853
                     /gene="blc"
                     /locus_tag="OO322_003663"
     CDS             6320..6853
                     /gene="blc"
                     /locus_tag="OO322_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5908386.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6850..7167)
                     /gene="sugE"
                     /locus_tag="OO322_003664"
     CDS             complement(6850..7167)
                     /gene="sugE"
                     /locus_tag="OO322_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5908387.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="OO322_003665"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="OO322_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5908388.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="OO322_003666"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="OO322_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5908389.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="OO322_003667"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="OO322_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5908390.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..>9137
                     /gene="epmB"
                     /locus_tag="OO322_003668"
     CDS             8385..>9137
                     /gene="epmB"
                     /locus_tag="OO322_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5908391.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLL"
BASE COUNT         2312 a         2364 c         2332 g         2129 t
ORIGIN      
        1 gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg cagatccgtg
     2281 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga tatcaatgag
     5881 aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac cggcgatatg
     5941 tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag catcattaac
     6001 ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa gcccccaact
     6061 cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt tggtagctat
     6121 gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa aaactatccc
     6181 aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta aaatttcatc
     6241 gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca cttagaaaaa
     6301 aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt
     6361 tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag tgaataattt
     6421 cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc accgttttga
     6481 acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt
     6541 cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag ggaaagcgta
     6601 ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg
     6661 cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt gcggcccgga
     6721 ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag tgaaacagga
     6781 gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt gggtacagca
     6841 gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga ttaacgccag
     6901 actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg tgatggccgc
     6961 gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta acgatttcat
     7021 cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa cactcggcgt
     7081 caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt ccagcagacc
     7141 agcaataact aagataatcc aggacatatc aggctccgga aatttggggc cgtccccggt
     7201 gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata ttttgccagg
     7261 tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa acggcacgac
     7321 actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgagagat
     7621 ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcattgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgctt
//
LOCUS       DAJTRM010000138         9111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRM010000138 DAJTRM010000000
VERSION     DAJTRM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.138"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..689
                     /gene="hchA"
                     /locus_tag="OO322_003669"
     CDS             <1..689
                     /gene="hchA"
                     /locus_tag="OO322_003669"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5908392.1"
                     /translation="ADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISG
                     LMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGA
                     LIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADK
                     QTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLA
                     AQEMLAAYAS"
     gene            complement(797..2155)
                     /gene="hprS"
                     /locus_tag="OO322_003670"
     CDS             complement(797..2155)
                     /gene="hprS"
                     /locus_tag="OO322_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5908393.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2155..2826)
                     /gene="hprR"
                     /locus_tag="OO322_003671"
     CDS             complement(2155..2826)
                     /gene="hprR"
                     /locus_tag="OO322_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5908394.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2959..3372
                     /gene="hiuH"
                     /locus_tag="OO322_003672"
     CDS             2959..3372
                     /gene="hiuH"
                     /locus_tag="OO322_003672"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5908395.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3481..4485
                     /gene="msrP"
                     /locus_tag="OO322_003673"
                     /pseudo
     CDS             3481..4485
                     /gene="msrP"
                     /locus_tag="OO322_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4486..5121
                     /gene="msrQ"
                     /locus_tag="OO322_003674"
     CDS             4486..5121
                     /gene="msrQ"
                     /locus_tag="OO322_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5908396.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5379..6029
                     /gene="zinT"
                     /locus_tag="OO322_003675"
     CDS             5379..6029
                     /gene="zinT"
                     /locus_tag="OO322_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5908397.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7107..7376)
                     /locus_tag="OO322_003676"
     CDS             complement(7107..7376)
                     /locus_tag="OO322_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5908398.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7433..8101
                     /locus_tag="OO322_003677"
     CDS             7433..8101
                     /locus_tag="OO322_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5908399.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8417..>9111
                     /locus_tag="OO322_003678"
     CDS             8417..>9111
                     /locus_tag="OO322_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5908400.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2611 a         2052 c         1862 g         2586 t
ORIGIN      
        1 ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc ggtaaccatc
       61 cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa ttcgaagtgg
      121 cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac aaagatgaaa
      181 aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag aaactcgcgg
      241 atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt cctggtgggc
      301 atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta cagtgggcaa
      361 tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt ctggcacttc
      421 gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac gccgcagaca
      481 aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc ggcgaagagt
      541 tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta cataaggacc
      601 gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa ctggcggcgc
      661 aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta agcgcataat
      721 caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt aacttatggc
      781 gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg ttatgcttac
      841 tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg actaaagaaa
      901 ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat ctacggaaga
      961 gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta agagagccgt
     1021 tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat cgaatggcgt
     1081 taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag atttgttgat
     1141 tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga tagtccaaaa
     1201 gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca ttgtttttgt
     1261 ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc agctcttcaa
     1321 tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg agcgtaacct
     1381 gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga tcgtcagcaa
     1441 attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc aacgcctgcc
     1501 caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg ctatcgttat
     1561 aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc gtcctgatta
     1621 acaacggact taatactgag cacagtataa tggcgacaat gcaaattata atgctattaa
     1681 ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca gtaaccgtta
     1741 atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta tcattaataa
     1801 tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta tttaacatgt
     1861 catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga atgatcaaaa
     1921 tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct ggatttaccc
     1981 catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta tcatcgcgcc
     2041 atttcaactc acttgccagg ccattataga gagtccatac aattccagcg ccagcaacag
     2101 actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt ttcattattt
     2161 ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct tttcaggaaa
     2221 aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat tggtatcact
     2281 atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa tctcgccagc
     2341 tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa tactgatatt
     2401 gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt ctaatgttga
     2461 attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact cagaaaatga
     2521 aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc tgtcatcgac
     2581 agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta acgtttgtaa
     2641 gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg cataatcatc
     2701 cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga cataacccgc
     2761 ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa taagtagaat
     2821 cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt tcattacaaa
     2881 attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa aactcattca
     2941 catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc aacggcagcc
     3001 ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt gcacattttg
     3061 aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa gaaagcggat
     3121 aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat taaggcactg
     3181 tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac cggggactat
     3241 ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt tcatattaat
     3301 aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta ttcaacctat
     3361 cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag ggaggtgact
     3421 ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt gtgatggctt
     3481 atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt attctttatg
     3541 aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc tttgcctcac
     3601 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc cgcccccgcc
     3661 ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc actgacgcca
     3721 gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa agacgatccc
     3781 gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagtg
     3841 gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct ggaagagcgt
     3901 atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat tggttttccg
     3961 ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
     4021 acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg cggcgggctg
     4081 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
     4141 accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt gcgactgatt
     4201 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccgc
     4261 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
     4321 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     4381 ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta cgccgaccag
     4441 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     4501 aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     4561 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
     4621 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     4681 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     4741 gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt gaataatctg
     4801 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     4861 gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa actcggcaaa
     4921 cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc aatacattat
     4981 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct ggctgtactg
     5041 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggcc
     5101 cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat cccggccgga
     5161 tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt tcgtttgtca
     5221 tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca atacacctga
     5281 gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt acaatataac
     5341 attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt cttcataaac
     5401 tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     5461 ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     5521 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttaccctt
     5581 tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca gataaaacca
     5641 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat atcgaaatga
     5701 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     5761 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     5821 tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt agcgatcata
     5881 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccagcaat
     5941 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     6001 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     6061 aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga caaactcaga
     6121 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     6181 gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     6241 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     6301 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     6361 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     6421 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     6481 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     6541 tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc acagtatcga
     6601 acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa
     6661 agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag ggggatttga
     6721 acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga gtcgaaaaat
     6781 caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg
     6841 aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac ctgttttcag
     6901 gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct cagcttattc
     6961 cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta acggctggaa
     7021 atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt cctgcatacg
     7081 tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata
     7141 tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc
     7201 agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca
     7261 atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc
     7321 tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
     7381 ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa
     7441 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
     7501 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
     7561 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
     7621 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
     7681 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
     7741 tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc
     7801 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
     7861 caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga
     7921 tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat
     7981 cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga
     8041 gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata
     8101 gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg
     8161 ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga
     8221 tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat
     8281 agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc
     8341 aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc
     8401 cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc
     8461 tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga
     8521 atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt
     8581 atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc
     8641 acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc
     8701 cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc
     8761 tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc
     8821 tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc
     8881 gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc
     8941 agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat
     9001 cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga
     9061 aaatcaacta tctcccagaa taatgcaaac tttgttacaa caaaccgccc a
//
LOCUS       DAJTRM010000139         9106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRM010000139 DAJTRM010000000
VERSION     DAJTRM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.139"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..818)
                     /locus_tag="OO322_003679"
     CDS             complement(<1..818)
                     /locus_tag="OO322_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5908401.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLR"
     gene            878..1156
                     /locus_tag="OO322_003680"
     CDS             878..1156
                     /locus_tag="OO322_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908402.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1157..1684)
                     /gene="gadE"
                     /locus_tag="OO322_003681"
     CDS             complement(1157..1684)
                     /gene="gadE"
                     /locus_tag="OO322_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5908403.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2483..3055)
                     /gene="hdeD"
                     /locus_tag="OO322_003682"
     CDS             complement(2483..3055)
                     /gene="hdeD"
                     /locus_tag="OO322_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5908404.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3310..3642
                     /gene="hdeA"
                     /locus_tag="OO322_003683"
     CDS             3310..3642
                     /gene="hdeA"
                     /locus_tag="OO322_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5908405.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3758..4084
                     /gene="hdeB"
                     /locus_tag="OO322_003684"
     CDS             3758..4084
                     /gene="hdeB"
                     /locus_tag="OO322_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5908406.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4148..4794
                     /locus_tag="OO322_003685"
                     /pseudo
     CDS             4148..4794
                     /locus_tag="OO322_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4836..5366)
                     /locus_tag="OO322_003686"
     CDS             complement(4836..5366)
                     /locus_tag="OO322_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5908407.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5522..6088)
                     /gene="slp"
                     /locus_tag="OO322_003687"
     CDS             complement(5522..6088)
                     /gene="slp"
                     /locus_tag="OO322_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5908408.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6335..7558)
                     /locus_tag="OO322_003688"
     CDS             complement(6335..7558)
                     /locus_tag="OO322_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908409.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8139..>9106)
                     /locus_tag="OO322_003689"
     CDS             complement(8139..>9106)
                     /locus_tag="OO322_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5908410.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2659 a         1803 c         1903 g         2741 t
ORIGIN      
        1 cgtaacgtca cggagcccgt ggtttcatca accgtcgggt cggagaattt cagcgtgccg
       61 gtctggctgt aacgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc
      121 tgtttgattt gcccactggc gacctcttct ttcatgcgta agaaatcttg caccgactgc
      181 gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta
      241 gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag
      301 gtgacattcg cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg
      361 acattggctt ctgcttcatt cagctgggtg cgcgcggtgt cgtaatcctg acggctaacg
      421 tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta
      481 gagagcgctt tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga
      541 tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta
      601 atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg
      661 ggcaattcgc tcaacacatt gaccgaacca ggggagagtg tgacgacacc gacctcaggc
      721 gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag
      781 aataacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga
      841 gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg
      901 catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt
      961 caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa
     1021 gggttctact ggtggataca cataccaggg gaatcaagtc gttttagcct ctcctttagt
     1081 aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca
     1141 gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac
     1201 gtcttagttt cagcttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa
     1261 tattgagcgt cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt
     1321 tgaactgatt atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag
     1381 taatctcaag aagatttttg atactttctt tgcggctaac aaaataaata ggcgctttag
     1441 cttttagttt actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct
     1501 ccagaaattt aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct
     1561 tgaagggttt attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt
     1621 gattatcttt caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa
     1681 tcataacttg ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat
     1741 ccttgtccga atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac
     1801 taatacgtct attaacacta atcaatactt cagatttatc catcacgcac tccatatatt
     1861 gatcctaagt aagattcctg gttgttatca gcttgtaaat agtaaacaaa aagttaacgt
     1921 ttgcggatta ctgcccaaga ataagttatc aataaccagt ttgtgatctc tgaagaatat
     1981 tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct ccttgtttcg acttaagctg
     2041 gcaattggat tgccagcttt cttttaacgc attgaaatta cctgagtaaa taacgcattc
     2101 atgtcttcag gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg
     2161 ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac
     2221 ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac tacggaaaat
     2281 atcagccatg ataatgtttg aaatggctaa tttgccatgg agtgaaaaaa attagatgaa
     2341 attcagtagg ttgaaataat cactagcagg taattatttc aatgatagtg cgcaattgat
     2401 ctacaacact gcgtagcgga gagagtatta atcggatcat agtcacatca agtgactatg
     2461 atccgggtga caaccggggt aattattgct gcttaacgaa caaactggcg aagctgaaca
     2521 ggctggcggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttacagaca
     2581 ccatcggcgt tgcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga
     2641 gctgtagcca gctgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc
     2701 ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctccggtg
     2761 cacggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata
     2821 ccggccagaa attatgactg cggttgctga ataacccgac aataagcgca atacccgagc
     2881 agattaataa tgcacccact actgtgctta aaatatcgcc agagacgaac gggaaactga
     2941 tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgct
     3001 ttttcagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaac
     3061 caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa
     3121 gaatgcagtc gatttaataa aaatttccta attgcagtat ctgatgcatc tgtaactcat
     3181 tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa
     3241 ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt tatagttgag
     3301 gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagtt
     3361 gtgagcaatg cagcggatgc gcaaaaagca gctgataaca aaaaaccggt caactcatgg
     3421 acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct
     3481 gaagcgctga acaacaaaga taaaccagaa gatgcggttt tagatgttca gggtattgca
     3541 accgtaaccc cagctatcgt tcaggcttgt actcaggata aacaagccaa ctttaaagat
     3601 aaagttaaag gcgaatggga caaaattaag aaagatatgt aattccggga atgcgttaca
     3661 tcgtacttcc ttgcatattg aacaggccgg aatatcttct ttaaaagcag ctattcctcc
     3721 tgttcatata taatctctat attgaatggg ttacaaaatg aatatttcat ctctccgtaa
     3781 agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgtt
     3841 ggcagccaat gaatctgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa
     3901 agcaatgacc ccggttgcat ggtggatgct gcatgaagaa acagtatata aaggtggcga
     3961 taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgtaa
     4021 gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa
     4081 ttaatgaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca
     4141 tttttatatg acagcagaat ttattatacg tcttatactc gcggcaattg cctgtggcgc
     4201 tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac atgtattaat
     4261 tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc
     4321 tttagatcac gtcggactcg accccagccg tattgctgct caggtggtga cgggcgtcgg
     4381 gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc tgacgacggc
     4441 agcggatatc tgggtgaccg ccgcataggt atggttattg gcagcggtat gtacgaactg
     4501 ggtatttatg gttcagtgat gaccttgttg gtgctggaag tcttccatca attaaccttc
     4561 cgcctgatga acaaaaatta ccatttacag ctgacattag tgaacggtaa tacggtatca
     4621 atgctcgact ggtttaagca gcaaaaaata aaaactgatt tagtttcgtt gcaggaaaat
     4681 gaagatcatg aagtggtcgc cattgatatc cagcttcatg ccacaacgtt gatagaagac
     4741 ctgttgcgat tattaaaagg tatggcaggg gtgaaaggcg tttcaattag ctaatctgtg
     4801 tggcagcgta gccagactca ccgtaagcct gaaattcaca ccagataatc aatatgctga
     4861 tggcgtacca gctcattgat ccgcttaaga ttgaggatca tcatgatatt gtgtttatgg
     4921 cagtagaacg tttttagcga tattttcagt ttctccagaa tatcttcagt tgtcatttga
     4981 ttcgcaatgc agctcagaac ctgatgttgc ttgcgcgaaa gtgacacatt cgcttcaggc
     5041 acagagcagt aactgccatt gatgagtgcg acaaaatcaa tgatagtgat agtccgcgga
     5101 acaaaggtca ccggtacttt ccccagacag cgtttaatat taaaggggga gaaaacaatg
     5161 acatggacag gttttaatcg ttcaatttga gtgagaaaat tagaatgaaa tacattattc
     5221 attaatgtat caataatgac gaaggcattc tgatgcaaca ttacgtcgat ctcttcttcg
     5281 ttctgtatat gaacgacatt acctttactc agaatgtttt tcatcgcggt gaagaacatc
     5341 gtatccctgg taattataag aaacataacc gactcctggt gcggacatta actttataat
     5401 cagtttgcca aaggtaatta acaggacagg gcttataata ataatagtta gcttactgta
     5461 tattaatttt atgtaataca tgatgcagat gctgagaaag catctgcatc tttcggtggt
     5521 gttatttgac cagctcaggt gttacctgac tcaccgcatt ggtgtagtaa ggcgcacccc
     5581 agcccggttc cggccagaat gcaccatagc cgtaatccca caggttatag gtggtattaa
     5641 ccacttctct caaatgccac acctggatgc cctgcatatt cacttccagg aagttatacg
     5701 ggactttatt gataaagcct ggttgttcac cctgaatggt accgaggatg gtaacaaagt
     5761 gattacgata gttcactgga tcaaggaagc cgctttgtct ggcgagcagt cggccctgat
     5821 agttggcttc aatatcaggc ttcgcatagc tatccaacgg taatacagcg atttctaaca
     5881 acgtatccgt tttgccattg ataacgttga taaccttccc accaaagcgc gcttgttgac
     5941 caacatataa ccccggctgg ttatgaacag caacaaaact tttttgaata tcaggttggt
     6001 tattgccttt gatattttgc ggaattgaac tacatgcggc aagcaaaaat gaaaggctga
     6061 ggatgagtgc accttttgtc atgttcatgt tactatcctt atcaacaaat attaaaacca
     6121 taatagatat ttctttatag ttttcatctg attctgaggt gatatgcctg atagtaacgt
     6181 aaacctgtct gatgtttata tcccttctgt ttagttcaat tccgttattt tggggcgtgg
     6241 tatgagtatc catcgcccaa taatataacc ctttgtttta aaattacata aaatctattt
     6301 ttattcaatg attataaagt gctaataatc aatattatgg tattatattc tgataatgat
     6361 ttgataagcg aattcttttg ccagatggta tttctatttc caccccataa tttatatctt
     6421 tttcattagg cagatctctc tttaaataaa aggggtatga aatcttttcg ggttgaagtg
     6481 tctcgtaatg aaattctatg aattcatttt tagacattgt tgcccatttt tgaattttat
     6541 tgtatagaga gggtgttgta aactcttgaa gttgtttata acatattttt ttgaactctt
     6601 gcttatattt taatatgtca tcaaatgaaa gattcactac ctttgcctct aagaaatcaa
     6661 ttattctcga agcaatttcc gctttcgcgc gtaaagagtt gtcattaata gttaacaatg
     6721 tagaaaacaa accattgatt tgtttgtcaa gatgttgttc atatttatgt agtttgggga
     6781 tgggattttc cttgtgtgaa aaaatagtgt taaataaatt tgcgtcagtg aaatttatat
     6841 taagtagatc ttcaaaataa aattttgata aggttaggac tagcttggac atatcttgcc
     6901 ctgtcatgtt gaaatcaata aagtcgcatc cagcaagggc ttccatccta ttttcatttt
     6961 tactgtgctc aatatacagc ttcgccattt ttaagtacaa gctttgaatg tctcttatat
     7021 cagttggtaa gtcagctcgt tgcccagcaa aatttttatt atcggaagaa acggtttgga
     7081 cactgaatct ttcgggtatt ccttcttcta cgagaatttt agttatgatt ggattgttgg
     7141 gttctggatt aacgacaaca atgatttgac atacagaatc cttatccttt tcgtatctaa
     7201 tgttatgaat ctgattaagt attgattgtg tctgttgcgt aagtggtgtc gaacttatcg
     7261 tttcaatgtt attaataaga cttacaagtt gaaccacaac ttttgagtag tctgcatttg
     7321 tatatgtggt gttcgtgtgg catgcattaa tttgttggag ttgttgttgc gctaaatcaa
     7381 attgttcagg gttgtatcct tctccagaga atgtggaatt aatgggttgt tgttcttccc
     7441 aattatcaca aggccacttc ggtgtgtctg gcgttttaac attatcggta tttgttgtgc
     7501 agttataaat atcgccgtga tatgtattta ttatacctgg ggtaaagtca attgacataa
     7561 cattccttat tcatcatctg ttttatgcta atgtttttaa attggataat tagaacagga
     7621 atggattttc ttctacacac aataaatata atgcgttata tcttcctgat ataattacct
     7681 ttaaattccc tttcatttcc acccggtaat atttgtgtat aaaaaaaggg cagtgatagt
     7741 atcgtaaagg aaaacgttaa caactatcaa aaaacgctca ttttaaaaag ttattatgtt
     7801 ctccgatagt gtgtgaagta atatttttgt gatgttttag atgtgtaata atgaaccagg
     7861 ttaaagtgta tttaaaaatg tgttgtatta gcgactgaaa tctgcctttt aaaaaggagg
     7921 agggtactga gacaaagagt cattgcttct ccgataaaat gtcagagaag catgattacg
     7981 tggttattgt ctgttgatga cttgttgacc gagccgtcat cattctcaat gcattagtta
     8041 aatcatttgt gatttttaag gattatcctg tctatgattt tctacttaga aggatatggc
     8101 actaactatg catcttatag tttactgctg gcatctattt aaaatagcaa ttgagtcaaa
     8161 tagcccatca agatagccat acctaatata accgtcagga atgcagctat catcggcatt
     8221 ctgaacattg atttaagtaa tatcacttcc gtcaggcttg cgccggcact gccgatgatt
     8281 aatgccatta atgctcccag acccattcct tttgtcatca gaacagatgc cagagggata
     8341 accgcctcag cccggatata cagcggaata ccgacaacgg cgcttaatgg gatggcgaag
     8401 ggattatctg ctcctgcatg agcggcaatc cacgctgagg gaataaagcc ataaataaaa
     8461 gatcctatta atactcctaa caaaagatat ggcagtacat ctttaaactg ttgcaacgca
     8521 tctttggtgg ctagttttat gggattaata cttactacag ctttggtact gcaacaggta
     8581 actacggggt tctcaatggc tttagccgct ggactacaac aactcacttt tatgggcgta
     8641 tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat ttgctgatga gcttttactg
     8701 gcaatgatat gacgttcaaa ccccagagaa tccaggataa tactggcaag aacggagacg
     8761 ccggctgcga taatcgcgta caacaaggtg actttccaac caaaggtcat ccacattagt
     8821 ccgacgataa tgggattaag taatggtgaa acaaataaaa aagtgagagt cggaccaaac
     8881 ccggctttcg ctgacaacaa tccacgtaac atgggtattg tcgagcaact acagaacggg
     8941 gtaacggctc ccaacagagc agccaggaga tagcctctcc cttttctggc ccccatcatc
     9001 tgctggattt tatggtctgg caccttttgt cttatcaggc tgacaccggc actaatcact
     9061 ataaacagca acgaaagctc gacggcgaga aacacaaaca tctctg
//
LOCUS       DAJTRM010000140         8973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRM010000140 DAJTRM010000000
VERSION     DAJTRM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.140"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..731
                     /locus_tag="OO322_003690"
     CDS             <1..731
                     /locus_tag="OO322_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5908411.1"
                     /translation="AGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQLQFSEW
                     LALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLAAAV
                     GMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMAL
                     FCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLA
                     LVIAACVTFTGALCYFFIVKDRIE"
     gene            983..1900
                     /gene="oxyR"
                     /locus_tag="OO322_003691"
     CDS             983..1900
                     /gene="oxyR"
                     /locus_tag="OO322_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5908412.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1883..3283)
                     /gene="sthA"
                     /locus_tag="OO322_003692"
     CDS             complement(1883..3283)
                     /gene="sthA"
                     /locus_tag="OO322_003692"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5908413.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3604..4254
                     /gene="fabR"
                     /locus_tag="OO322_003693"
     CDS             3604..4254
                     /gene="fabR"
                     /locus_tag="OO322_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5908414.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4254..4613
                     /locus_tag="OO322_003694"
     CDS             4254..4613
                     /locus_tag="OO322_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5908415.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4653..5753)
                     /gene="trmA"
                     /locus_tag="OO322_003695"
     CDS             complement(4653..5753)
                     /gene="trmA"
                     /locus_tag="OO322_003695"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5908416.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5867..6057
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6122..7966
                     /gene="btuB"
                     /locus_tag="OO322_003696"
     CDS             6122..7966
                     /gene="btuB"
                     /locus_tag="OO322_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5908417.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7911..8768
                     /gene="murI"
                     /locus_tag="OO322_003697"
     CDS             7911..8768
                     /gene="murI"
                     /locus_tag="OO322_003697"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5908418.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2055 a         2172 c         2416 g         2330 t
ORIGIN      
        1 tggcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg gcgcagtttg
       61 tcctgactga cgaagaacgg acgtatctct ccgcgtcggt taaaccgcgt ccacagctgc
      121 aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg attttaggtt
      181 tctctggcgt caattatacc ggttggcttt acattgcgtg gttacccggt tatttgcaag
      241 ccgaacaagg tttcagtctg gcgaaaaccg gttgggtagc ggcgattcct ttcctcgcgg
      301 cggcggtcgg gatgtgggtt aacggtattg tggtcgatcg gctggcgaaa aaaggctacg
      361 acctggcgaa gacgcgtaaa acggctattg tctgcggttt gatgatgtcg gcattaggca
      421 cgttgctggt cgtgcaatct tcctcgccag ctcaggcggt agcgtttatc tcaatggcgc
      481 tgttctgcgt gcatttcgcg ggaacgtctg catggggact ggtgcaggtg atggtgtcag
      541 aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt tttgcttcct
      601 ttgctccgat cgtaaccggt tgggtagtgg ataccacaca ctcgtttaat ctggcgctgg
      661 ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt gtcaaagatc
      721 gcattgagta atcgttacta tcaggctctc ttgctgtggg cctgtagaat aaaaaaaagc
      781 ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg agaaactctc
      841 gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt gagcaattat
      901 cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg ccatgaacta
      961 tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg tggcattggc
     1021 tgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc cgacgcttag
     1081 cggccaaatt cgtaagctgg aagatgagct gggcgtgatg ttgctggagc ggaccagccg
     1141 taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta ccgtgctgcg
     1201 tgaggtgaaa gtccttaaag agatggcaag ccagcagggt gagacgatgt ccggaccgct
     1261 gcatattggt ttgattccta cggttggacc atacctgcta ccgcatatta tcccgatgct
     1321 gcaccagaca tttccaaagc tggaaatgta tctgcatgaa gcacagaccc accagttact
     1381 ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga aagagagcga
     1441 agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct atgaagatca
     1501 cccgtgggcg aaccgcgaat gcgtactgat ggccgatctg gcaggggaaa aactgctgat
     1561 gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgctttg aagccggggc
     1621 ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca tggtggcggc
     1681 aggtagcggg atcactttat tgccagcgct ggctgtgccg ccggagcgca aacgcgatgg
     1741 ggttgtttat ctgccatgca ttaagccgga accatgccgc actattggcc tggtttatcg
     1801 tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc gcgcaagaat
     1861 ggatggccat ttcgataaag tgttaaaaca ggcggtttaa accgtttaac gcagctaccc
     1921 gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt
     1981 tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg gcagcgcgct
     2041 cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa attttcagcg
     2101 tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttgaactgg gcgcggccca
     2161 cttcatacgg cactttcatc gcggtcagct gctgttcggt tttgcccaca gagctgattt
     2221 ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgtgcggtg gcttcgcctt
     2281 ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgctgatgcc aggctcggat
     2341 aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga
     2401 ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg
     2461 tattaccggt acgaccgttg gcatagagca ggcagtcagc tttcagtttt ttacccgact
     2521 tcagatgcat gatcacaccg tcgtcacagc tttcgatctt ctcgtactct tcgttgtgac
     2581 gaatcactac gccactgttc cagaagtggt aggagagaga atctgacatc tcttgatcga
     2641 gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc ataccgcgga
     2701 agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca tggcgcggtt
     2761 cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgggtg aaatcaacat
     2821 ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg gttagtgttt
     2881 caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag cgagcgttcc
     2941 cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat
     3001 taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc agtcgggaat
     3061 ggtcgctgta tagtgggttt tgattgaatt ctataatgcg gctgacggcg tgacggagag
     3121 ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga taacgctcga
     3181 taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg ccgccggggc
     3241 cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt
     3301 cttatacata aaagcaacag aatggtaaca ttctatcgcg ggtaagccaa ttgatccccg
     3361 tcatttatct ggctatatcc tgagcggcct ttgctttgtc tgtttcttac ttttgccctg
     3421 acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt tccgctgtac
     3481 gtaaaagaac cggccagtaa atatgtttta ttgcgttacc gttcattcac aatactggag
     3541 caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa ggattcagac
     3601 atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagcc
     3661 gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg
     3721 cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt agacgaactg
     3781 ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg ccaggcgcgt
     3841 cagcgtatcg ccaaaggcgg gagtgtgatc cgtacctcgg tctccacatt tatggagttc
     3901 atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg cacctccgct
     3961 gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact tgcggactat
     4021 ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga agcaatggtg
     4081 acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg gcgtcgaaca acgtcggcaa
     4141 ttagaagagc gactggtact gcaactgcga atgatttcga aaggggctta ttactggtat
     4201 cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac
     4261 aagcaaatca agatagaggt acgctgctgc tggcgttggt tgctggttta tcgattaatg
     4321 gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg attatttccc
     4381 tggtgctgac ggtttactgc ctgcatcaac gttatcttaa tcgcaccatg tcggtaggct
     4441 tgccgggtct ggcagctgcc tgttttattc tcggcgtact gctgtacagc acggtagttc
     4501 gtgcggaata tccggatatc ggctctaact tcttcccggc agtactctcc gtcattatgg
     4561 tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag taatcggtat
     4621 catgccggat gctggcgcat ccggcatggg ttttacttcg cggtcagtaa tacgccgcac
     4681 tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcgac cttgtgcgtc
     4741 tggcttaatg tttccagatt tttgcataac gtttccggat tgcaggagat atacagaata
     4801 cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg cggtgggtcg
     4861 acaaaaatgg tttcgaactg atagctcttt aagtcgatcc cttgcaggcg gttaaactcg
     4921 cgcacaccat tcatcgcctg agtaaattct tctgccgcca tacgaataat ttgtacgtta
     4981 tcaatatggt tagcggcaat gttgtattgc gcggcagcga ctgacggttt ggcgatttcg
     5041 gtggctaata cccgatcaaa attgcgcgcc agcgctaatg aaaagttacc gttgccgcag
     5101 tacagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca ttccagcatc
     5161 tgaatattca tcgccgcgtt cggctgggta aagctgtttt ctacctgacg atagatcatc
     5221 tctttccctg cgaccggcag acgctcgtcg atgtaatctt gatccagcgc gattttggtt
     5281 ttcgttgccc gaccaatcag atgcacattc agattctgcg cgcgcagtgc atcgcgcagt
     5341 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5401 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5461 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5521 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5581 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5641 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5701 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5761 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     5821 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     5881 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     5941 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6001 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6061 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6121 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6181 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6241 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6301 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6361 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6421 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6481 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6541 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6601 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6661 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6721 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6781 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     6841 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     6901 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     6961 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7021 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7081 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7141 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7201 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7261 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7321 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcactaa atctggggca
     7381 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7441 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7501 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7561 gattaagggt gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7621 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7681 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7741 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7801 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     7861 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     7921 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     7981 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8041 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8101 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8161 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8221 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8281 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8341 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8401 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8461 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8521 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8581 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8641 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8701 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8761 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     8821 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     8881 gggtcagcgg ggttctcctg agaattccgg cagagaaagc aaaaataaat gcttgactct
     8941 gtagcgggaa agcgtattat gtacaccccg cgc
//
LOCUS       DAJTRM010000141         8952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRM010000141 DAJTRM010000000
VERSION     DAJTRM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.141"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..313)
                     /locus_tag="OO322_003698"
     CDS             complement(<1..313)
                     /locus_tag="OO322_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908419.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SL"
     gene            complement(400..761)
                     /locus_tag="OO322_003699"
                     /pseudo
     CDS             complement(400..761)
                     /locus_tag="OO322_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1100..1654)
                     /locus_tag="OO322_003700"
                     /pseudo
     CDS             complement(1100..1654)
                     /locus_tag="OO322_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1797..1919
                     /locus_tag="OO322_003701"
                     /pseudo
     CDS             1797..1919
                     /locus_tag="OO322_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2181..2747)
                     /gene="ipgB2"
                     /locus_tag="OO322_003702"
     CDS             complement(2181..2747)
                     /gene="ipgB2"
                     /locus_tag="OO322_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5908420.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3029..3343)
                     /locus_tag="OO322_003703"
     CDS             complement(3029..3343)
                     /locus_tag="OO322_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908421.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3421..3531)
                     /locus_tag="OO322_003704"
                     /pseudo
     CDS             complement(3421..3531)
                     /locus_tag="OO322_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3855..4532)
                     /gene="ospD1"
                     /locus_tag="OO322_003705"
     CDS             complement(3855..4532)
                     /gene="ospD1"
                     /locus_tag="OO322_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5908422.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4904..5041)
                     /locus_tag="OO322_003706"
                     /pseudo
     CDS             complement(4904..5041)
                     /locus_tag="OO322_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5123..5421)
                     /locus_tag="OO322_003707"
                     /pseudo
     CDS             complement(5123..5421)
                     /locus_tag="OO322_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6010..6169)
                     /locus_tag="OO322_003708"
                     /pseudo
     CDS             complement(6010..6169)
                     /locus_tag="OO322_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6173..7402)
                     /locus_tag="OO322_003709"
                     /pseudo
     CDS             complement(6173..7402)
                     /locus_tag="OO322_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7422..7769)
                     /gene="tnpB"
                     /locus_tag="OO322_003710"
     CDS             complement(7422..7769)
                     /gene="tnpB"
                     /locus_tag="OO322_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5908423.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7769..8142)
                     /locus_tag="OO322_003711"
                     /pseudo
     CDS             complement(7769..8142)
                     /locus_tag="OO322_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8212..>8952
                     /locus_tag="OO322_003712"
     CDS             8212..>8952
                     /locus_tag="OO322_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR5908424.1"
                     /translation="MFPDSFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNGWSLAGEHRHWMIPLRKGAQYE"
BASE COUNT         2269 a         2134 c         2004 g         2545 t
ORIGIN      
        1 agagagaacg aatgaatgcc ctgagaatgg tcattccgcc acgatatccc tggtctctga
       61 tctcgcgagc gattaccgtt gccgggattt tgtaaggatg agcatcggcg atgcgttgac
      121 gaatataatc ccggtattca tccaggagtg aagcaacagc aggtcgcggc gtatattttg
      181 gcggctcaga ttttgcctgc aaataacgtt taacggtatt gcgggagagc cccagttctc
      241 tggcaatcgc ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct
      301 caaaagtgac cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc
      361 aggcgttggg tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc
      421 aacgaagctc cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg
      481 cgcagatgtt atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg
      541 attttcagcg tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt
      601 tggtttttaa ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga
      661 cagtcacggc aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga
      721 acggactggc aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg
      781 tattataacc cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact
      841 taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt
      901 atcataatga tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa
      961 tgcacagtgc ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga
     1021 caacagcatc tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc
     1081 acgtaatatg gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc
     1141 cgccacacca actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg
     1201 ttgccctcat tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg
     1261 aatgaaagaa gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc
     1321 cacgcagatt atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc
     1381 ggtccgagtg aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg
     1441 catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg
     1501 accacaggtc aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga
     1561 tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt
     1621 ctgacacagg caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc
     1681 tcactggtat ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt
     1741 cacgtacggt tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag
     1801 gttccgacag cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc
     1861 tgaacaatgt ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac
     1921 cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat
     1981 caatatgatt ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat
     2041 ccatgagcta ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact
     2101 tgcgaggaga aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct
     2161 ttactcggga tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata
     2221 ttcccacgta atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa
     2281 ataagatttg caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa
     2341 agttcgtcca ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt
     2401 tgtgcagcat tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt
     2461 ttacggagat tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg
     2521 tttaccaccc gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca
     2581 ccgatttgtt ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt
     2641 actatccttt tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt
     2701 ttgtgagata aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg
     2761 tcatgtaaaa tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt
     2821 tgagttcttt actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct
     2881 ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt
     2941 aaaataaccg gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc
     3001 attaaaattg ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc
     3061 gatacatcac tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa
     3121 aacgccgttt tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga
     3181 taaaaccttg gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga
     3241 gattcgctct tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg
     3301 gcagctgagc aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg
     3361 acgtagccgc tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc
     3421 ttaagtgagg ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg
     3481 tcgtcagcga ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt
     3541 ctccagtctg agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca
     3601 aggctccaca ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt
     3661 gggatgtgtt cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc
     3721 gtcatgggaa actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt
     3781 ttagaaatta agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca
     3841 gctatttagc acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct
     3901 tgttcattat tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat
     3961 atatttatat atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct
     4021 ggtaacccct cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca
     4081 aaagttttaa taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct
     4141 atgaacaaag cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct
     4201 ttgggtattt cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca
     4261 tgccaaatca atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg
     4321 gatatgttct gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat
     4381 ttttcttcat tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc
     4441 attcctttat tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt
     4501 gctggatgta atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa
     4561 acaataacct atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat
     4621 tgccacattg aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc
     4681 gtagatatat cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg
     4741 ccttcactca gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg
     4801 ccatacgcca gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc
     4861 cagctccggc ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac
     4921 tatgtttttc tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca
     4981 gtggccatat tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca
     5041 tcactgacag cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc
     5101 gcccgacaaa tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc
     5161 cattacgatc acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg
     5221 ttaagcaacc attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat
     5281 gatgagtcat attagcattg agtacggttt cattttcagc atccaatcaa actgactgag
     5341 atcttcagga tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga
     5401 tgtcaactgt ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac
     5461 atcagagaat tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat
     5521 agtgaaaata agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg
     5581 atgacctgat taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt
     5641 aatacatcac ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt
     5701 tagtcctttt gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt
     5761 tggataaact attgttctta ttccactaat acaaactttt tgtaaagtat attaaatata
     5821 cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc ataaaaggaa
     5881 gagggggccc taattaagtt gtcaagcatg tttatgatac ctgcagggta taaattttct
     5941 ggctgcaagc agacacatat aaaacgtctt tattgatcct acccacataa tatggacaca
     6001 gtcgtaagtg aggctctggt tttcaaattg ttccggactg agaccaccac accaacggtg
     6061 ccgtcgccag cgattgtaat cgcactcgat ataactaaac actgttgccc gcactatttc
     6121 ccgatggata atagtgttct ccgtggagac attccatttt cagcgaatga attttcatct
     6181 gttgctgtat ccagtcatac agtgactgca tcagtggcgc ggctctggct tttcttgccg
     6241 ccagacgctg ttctgctgaa catccccgga cttctgcctc gatggcatac agttcaccga
     6301 tacgctgcag ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt
     6361 ttctccggac atgagccata catgcggctt ccgttattct gccggattcg tacaacgccc
     6421 ggtaaccacc gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg
     6481 gatggatacc tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac
     6541 cggcgttacg gtcatcacga acgtagaccc acagccgggc agtccttgtt ttaccgctgc
     6601 ccggctcctg gaccggaacg gggatatcat cggcatggac tttaccgggc atcagcacat
     6661 actggcgcag gatgtcatac agcggttcca gcagttcagc aacagcacct gtccagcgcc
     6721 ccagtgtggc acggctcagc tccacgccct gacgacggta tatttctgac tggcggtata
     6781 acggcagata gtctgcatat tttccagcaa caacatgggc cagaagcccc gctccggcat
     6841 aactgcgtgc aatgggtttt gaaggcaccg gtgcctgcac gatatggtca caccggcaac
     6901 aggccagttt cggacgttgt gtttcgataa ccttaaaggc gctgctgata agctccagct
     6961 gctctgacac atcacatccc agagaactga gttcgccacc acaggccggg cagcattcct
     7021 cttccggacg gatgacccgg gtttcacggg gaagtgaggc tggtaacggt ttacgggctg
     7081 aagactggcg caggggagat ggcagtaccg ggtcatattg ctcacccagc gtttccgcca
     7141 tttcttcctg aagtgcgctg attcgctcct gtgcttccag tatctgccgt tcggtttttg
     7201 cgcgaagttt ttctgagctt ttaccgaact gcatgcgctg cagtttcgca accagcgcct
     7261 tcagccggtt gatttcggaa gcataagccg ccacccgctg tgagagcagg cggttgtatt
     7321 tcgccatctg gcggatggtg tcctgttgcg tctgcaacag tgcccgcagg cgggcattct
     7381 catgagcaag tgaggtgtcc atatcctcac tttacaacgg gttatatgcg gatgccagcg
     7441 cgttccgttc gtttcgggtg tttccagtcg atgccctcaa gaagcatgga taactgggcc
     7501 ggagtaaggt gcaccttgcc gtcacgggtg accggccaga cgaagcggcc ccgctccagg
     7561 cgtctggtaa agaggcacag tccgtcactg tcagcccaca acacttttat ctggtcaccc
     7621 cggcgtccgc ggaagatgaa cagatgcccg gagaacgggt catccttcag gacgttctga
     7681 acttttgaga ccaggccgtt aaagccattt cgcatatcgg tgatacctgc aaccagccag
     7741 atacgcgaac ctgcagggaa agatatcatc agtggctgct cccttttatt tcgcggataa
     7801 gtatctgtaa taacgccggc gtcagtttac ctttaagcct gagagttccg gccggcagaa
     7861 ccagctcaca gcacagactg tcggacggtg tatttatctg ctctggttcc tgtgccggag
     7921 ccgggatttt attatccggc tccggcgtta acgtcacggg aagcagtgca ggcatatttt
     7981 tccggaaggc agcaggccac ctttccggta ttgatggcgc cagttgaaga gcaggttatc
     8041 gttgattccg ttttcacggg cgatctgcgc cacacaggct ccgggctgca gtgactgctc
     8101 cactaaagcg attttaaatt cataaggaaa attaggccgc cggtgtaatg ccgatcagtt
     8161 aaggatcagt tgaccgatcc agtggctgtg taagaatccg gaaacgctca cttgtttccg
     8221 gattctttta tgcacattgg acaggctctt gatctggtat cccgttacga ttctctgcgt
     8281 aacccactga cttctctggg ggattacctc gaccccgaac tcatctctcg ttgccttgcc
     8341 gaatcaggta ctgtaacgct acgcaagcgc cgtcttcccc tcgaaatgat ggtctggtgt
     8401 attgttggca tggcgcttga gcgtaaagaa cctcttcacc agattgtgaa tcgcctggac
     8461 atcatgctgc cgggcaatcg ccccttcgtt gcccccagtg ccgttattca ggcccgccag
     8521 cgcctgggaa gtgaggctgt ccgccgcgtg ttcacgaaaa cagcgcagct ctggcataac
     8581 gccacgccgc atccgcactg gtgcggcctg accctgctgg ccatcgatgg tgtgttctgg
     8641 cgcacaccgg atacaccaga gaacgatgca gccttccccc gccagacaca tgccgggaac
     8701 ccggcgctct acccgcaggt caaaatggtc tgccagatgg aactgaccag ccatctgctg
     8761 acggctgcag ccttcggcac gatgaagaac agcgaaaatg agcttgctga gcaacttata
     8821 gaacaaaccg gcgataacac tctgacgtta atggataaag gttattactc actgggactg
     8881 ttaaatggct ggagcctggc gggagaacac cgccactgga tgatacctct cagaaaggga
     8941 gcgcaatatg aa
//
LOCUS       DAJTRM010000142         8906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRM010000142 DAJTRM010000000
VERSION     DAJTRM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.142"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..240)
                     /locus_tag="OO322_003713"
                     /pseudo
     CDS             complement(1..240)
                     /locus_tag="OO322_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            244..819
                     /locus_tag="OO322_003714"
                     /pseudo
     CDS             244..819
                     /locus_tag="OO322_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            870..1820
                     /locus_tag="OO322_003715"
                     /pseudo
     CDS             870..1820
                     /locus_tag="OO322_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2223..2804)
                     /locus_tag="OO322_003716"
     CDS             complement(2223..2804)
                     /locus_tag="OO322_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908425.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2851..6708)
                     /gene="sigA"
                     /locus_tag="OO322_003717"
     CDS             complement(2851..6708)
                     /gene="sigA"
                     /locus_tag="OO322_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5908426.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(7069..8073)
                     /locus_tag="OO322_003718"
                     /pseudo
     CDS             complement(7069..8073)
                     /locus_tag="OO322_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            8058..8435
                     /locus_tag="OO322_003719"
                     /pseudo
     CDS             8058..8435
                     /locus_tag="OO322_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8474..8821)
                     /locus_tag="OO322_003720"
                     /pseudo
     CDS             complement(8474..8821)
                     /locus_tag="OO322_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         2211 a         2061 c         2121 g         2513 t
ORIGIN      
        1 caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc gcccgcaagg
       61 cattttcagc gatgttgttg tcgatttcca cccagccatc gttcgcatag tacgtcagtg
      121 ccggccactg gttaagtgcg tacgcgaacg ccttcgccaa ctctgagtgt cgcgactggg
      181 tcttcatctt ttccgcaacc agctttccag ggatttcaac agcggtttcg tttttcgctg
      241 acaccatgga gggagtggtt aaaagagcaa cgagaaagtg gtaattataa cgccagccgg
      301 atatggcggg aaatggtggc ccggggattt acaggcagtg aaaccatcgt cagggatgcg
      361 gttgccaaat ggcgtaaagg ctggaaccca ccggttacta ctgccgtcag acttccttca
      421 gtctcccggg taagccgctg gttgatgccc tggagaataa ccagggatga agaaaattat
      481 gcttcccgat ttattagtct gatgtgtgaa aaagaacctg agctgaaaat agcgcagcaa
      541 ctggcactcg agttctaccg tattctgaaa acccaaaata aatcacagct aagcagttgg
      601 ttcactcgag tccacgaaag cggttcagca gaatttcggc gcgtggcggc ggggatggaa
      661 gctgatgctg cagctatatg tgaggcaatc agtagtcgct ggagtaatgg tgttgtcgaa
      721 ggtcatgtaa atcgcctgaa gaagatgctg aaacgccaga tgtatggtcg agcaggattt
      781 gaactactca ggcagagggt aatgagtcca ctggcatgaa aatctccacc caggttgcgg
      841 aagagccact tttcatccgt cgttgacagt tataccgcca gtcagaaata taccgtcgtc
      901 agggcgtgga gctgagccgt gccacactgg gtcgctggac cggtgccgtt gctgaactgc
      961 tggagccgct gtatgacgtc ctgcgccagt atgtactgat gcccggtaaa gttcatgcgg
     1021 atgatatccc cgttccggtc caggagccgg gcagtggtaa aacaagaact gcccggctgt
     1081 gggtctatgt ccgtgatgac cgtaacgccg gttcacagat gcccccggcg gtctggttcg
     1141 cgtacagccc ggaccggaaa ggtatccatc cacagaatca cctagccggt tacagcggtg
     1201 tgcttcaggc agatgcttac ggtggttacc gggcgttata cgaatccggc agaataacgg
     1261 aagctgcgtg tatggcccat gcccggagaa aaatccacga tgtgcatgca agagtgccaa
     1321 ccgacatcac cacggaagcc ctgcagcgta tcggtgaact gtataccatc gaggcagaag
     1381 tccggggatg ttcagcagaa cagcgtctgg cggcaagaaa agcaagagcc gcgccactga
     1441 tgcagtcact gtatgaccgg atacagcaac agatgaaaac actgtcgcgt cactcggata
     1501 cggcaaaagc gttcgcatac ctactgaaac agtggaaggc cctgaacgtg tactgcagta
     1561 atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cctgcgggcg gtcagtctgg
     1621 gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcggggc gcgctactgt
     1681 acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac cttcgccatg
     1741 tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt ccgtggcgca
     1801 tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta cgcttactca
     1861 tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac cctactcagt
     1921 acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca aaataaatca
     1981 accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct gaaaaacgcg
     2041 tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca acacgctcat
     2101 gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg cgtttataca
     2161 aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata ccgggacaga
     2221 gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct tactttacct
     2281 ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa tgtcacggat
     2341 gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat gagctgagat
     2401 ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat gtggcgcgta
     2461 tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag catgtgggag
     2521 acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat gataatggtc
     2581 ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa ccatgagaac
     2641 tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat agtttctttt
     2701 tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa aagaaagcta
     2761 ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc tccggtagaa
     2821 gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt tggcgttgat
     2881 cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac cgaagcgaag
     2941 attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt ctttttcacc
     3001 tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg caaacaggtc
     3061 aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg agaaggattt
     3121 acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct tcatggtcac
     3181 gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat aaaccagttc
     3241 tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg cttcagcacc
     3301 cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg tcgccgagta
     3361 ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag agtcaaatat
     3421 agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg cattactgct
     3481 ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc catccagctc
     3541 atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc caccagaagc
     3601 tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg tgtcacgcag
     3661 atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt aattaccgga
     3721 catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat atccggtcag
     3781 ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata tgttgctgaa
     3841 acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg gggctgttat
     3901 caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga ccgacagact
     3961 gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat ttacagactg
     4021 gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt ttactgtcac
     4081 agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc gggtttttaa
     4141 tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc tggctgaggc
     4201 tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca aacctgtagc
     4261 cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa caccaaacag
     4321 aatagatgct tttttgtctg attttatatt accattgacc caggcataat tttgagcctt
     4381 taacgtggca ccatcttctg tcatattata tccatctgac atatagattg ccgggctgta
     4441 tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt caatagcttt
     4501 atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa tcaggtctgt
     4561 attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa cagttatttt
     4621 gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat ccccggagcg
     4681 aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga acatgtcaat
     4741 gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt gtccttttac
     4801 attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc ggaaattaaa
     4861 tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg acttatattc
     4921 cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag aatagtcttt
     4981 ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg catgttctgt
     5041 cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt tgatatcgac
     5101 tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat tgataatatg
     5161 atttatgctt atattacctg atacattccc atggtacata tatgcatcag tattagtaag
     5221 tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat ctgttgctgc
     5281 gattttctga aatgactggt catagccgtt tagatccagt gttccgccat ttctggtaaa
     5341 atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt ttaccgtacc
     5401 tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga tgacagtccc
     5461 attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac cactacctat
     5521 tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg tattattatc
     5581 aatatctatc ccggcacctt tataagactt atcttttccg tacactttat atgtctggtt
     5641 cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg ttaatacccc
     5701 tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt gttttttacc
     5761 atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa tattattaaa
     5821 taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg tactggtaac
     5881 accatggaaa acccattttt gttgttcttt atcatataga taagaaccag aaccactatc
     5941 gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac tcttgttttt
     6001 ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt gtccaaacag
     6061 atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga agccaattat
     6121 ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg gagaaagtga
     6181 gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca gacgatgaac
     6241 cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat cccatgactg
     6301 tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt acgcacctcc
     6361 aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag gtagttctgg
     6421 aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac ccggtctgaa
     6481 ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat ttatatccag
     6541 cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa taataccgag
     6601 gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag aaacagcaac
     6661 taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac gtgaactccg
     6721 atatttctga gttgtggtta tgggatagca tttaattaca acggggaact atcagtcatg
     6781 gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta tgacagatgt
     6841 aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg atcacttggt
     6901 gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt caatccttta
     6961 caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga cttttgatga
     7021 tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc agaaggtgaa
     7081 ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg caacatcaag
     7141 gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc cgtcgttacc
     7201 ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat taaattctgt
     7261 gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg gggtcagatc
     7321 cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg tcacctgcgg
     7381 gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac tacgggagat
     7441 caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac tggcaccgat
     7501 gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg cgctgctgtc
     7561 ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt tgggggggaa
     7621 gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa tacccacatc
     7681 agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt ccacctcctg
     7741 cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga ctttaccgtc
     7801 gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat caccgggaag
     7861 ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga agtcccggag
     7921 gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga tggcgcggtc
     7981 gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat ccggattgga
     8041 gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc aaaatccacg
     8101 atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta atcggtgaac
     8161 tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg gcggtcaggc
     8221 aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag aaagaacaca
     8281 cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag cactgggctg
     8341 cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca gcagaaagag
     8401 cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat tccgtgtctg
     8461 actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca gcaagtgcaa
     8521 tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact tgtgattttt
     8581 tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct ttccatgcaa
     8641 tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat gctccaactt
     8701 ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt tcgctgtttc
     8761 caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca cctggcacca
     8821 gtctgaggtg tactggcaat agcggacact accatttgtt ctttttttaa gcagccatct
     8881 gatgatattt ttccctgaag gctgcc
//
LOCUS       DAJTRM010000143         8813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRM010000143 DAJTRM010000000
VERSION     DAJTRM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.143"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1257)
                     /gene="trkA"
                     /locus_tag="OO322_003721"
     CDS             complement(<1..1257)
                     /gene="trkA"
                     /locus_tag="OO322_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5908427.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDV"
     gene            complement(1279..2568)
                     /gene="rsmB"
                     /locus_tag="OO322_003722"
     CDS             complement(1279..2568)
                     /gene="rsmB"
                     /locus_tag="OO322_003722"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5908428.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2614..3561)
                     /gene="fmt"
                     /locus_tag="OO322_003723"
     CDS             complement(2614..3561)
                     /gene="fmt"
                     /locus_tag="OO322_003723"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5908429.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3576..4085)
                     /gene="def"
                     /locus_tag="OO322_003724"
     CDS             complement(3576..4085)
                     /gene="def"
                     /locus_tag="OO322_003724"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5908430.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4250..5374
                     /gene="dprA"
                     /locus_tag="OO322_003725"
     CDS             4250..5374
                     /gene="dprA"
                     /locus_tag="OO322_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5908431.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5346..5819
                     /locus_tag="OO322_003726"
     CDS             5346..5819
                     /locus_tag="OO322_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5908432.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5848..6390
                     /locus_tag="OO322_003727"
     CDS             5848..6390
                     /locus_tag="OO322_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5908433.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6395..6967
                     /gene="tsaC"
                     /locus_tag="OO322_003728"
     CDS             6395..6967
                     /gene="tsaC"
                     /locus_tag="OO322_003728"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5908434.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            6972..7790
                     /gene="aroE"
                     /locus_tag="OO322_003729"
     CDS             6972..7790
                     /gene="aroE"
                     /locus_tag="OO322_003729"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5908435.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7787..8044
                     /locus_tag="OO322_003730"
     CDS             7787..8044
                     /locus_tag="OO322_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5908436.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8020..8574)
                     /locus_tag="OO322_003731"
     CDS             complement(8020..8574)
                     /locus_tag="OO322_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5908437.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2189 a         2155 c         2208 g         2261 t
ORIGIN      
        1 cacgtcgttt ccacgtacca ccgctccaat aatcgttcct ggcggtagct tgatttcgtc
       61 aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg tgagcaacgg cttcaatagc
      121 ttctgctacg ccgcggcgca atgaggaaac accaacaata tctgcttttc gcacatggct
      181 aagcaacgca gaaatagttg cttgttgtgg tgaaattgca atatcgatca cgctcccctg
      241 aaccagatcc acataagcgc gacgctggat caataccatc acctttttcg cacccatacg
      301 tttggcaagc atggcggaca tgatattggc ctcgtcatcg ttggtgacgg caataaacag
      361 atcaacttga tcgatatgtt cttcggccag cagttcttga tccgacgcat caccaaaaaa
      421 gacgatcgta ttctgcaact tttccgccag ttcggcagcg cgctgctgat tacgttcgat
      481 gagtttaacg ctgtaatctt tttccagacg acgcgccagc cctgcaccga tattaccgcc
      541 accaaccagc atgatccgtt tatacggttt ttccagtcgc tgtaattcac tcatcaccgc
      601 gcggatatgc tgtgaagcgg caataaagaa cacttcatca ccagcttcaa caatggtcga
      661 accttgtgga cgaatggggc gatcgtggcg gaaaattgct gccacacgag tatcgatatg
      721 tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc agcgggccgc cataataggc
      781 tttaactaca gcgaggctga ctttaccctc agcgaagttc accacctgca atgcgcctgg
      841 atactcaatc agtcggtaaa tattatcgat aaccaactgc tctggtgcga tcagatgatc
      901 aatcggtaca gcatctgaat gaaatagctt atcggcatcg cgtacgtagt ctggtgagcg
      961 gatgcgagcg atgcgattag gggtgttgaa aagtgagtag gctacctggc aggcaaccat
     1021 attggtttca tctgaactgg ttacagcaac cagcatatcg gcgtcgtcgg cacctgcctc
     1081 ccgcaatacg cgtggatgag agccatgccc ctgcacgact cgcaggtcaa atttatcctg
     1141 caaggtccgc agacgctcac cgttggtatc gacaacagta atatcgttgt tctcgccaac
     1201 caggttttcc gccagtgtgc cgccaacctg gccggcaccc agaatgataa ttttcatcag
     1261 tcgcgacccg ttatctcatc actttttgat tagcttagcg taaaagaagc cgtcgccctc
     1321 ttcggcacca ggctgatttt gtttacccgg ttgctctggt gttcctgttt cgcaaagttc
     1381 ggcatcagcg gtacgttgca aaaaggcttt aatctgcagg ctattctctt ccggtaacac
     1441 cgaacaggtg gcatagacca gggttccacc ggattttaaa tgcggccaaa tggcgtcgag
     1501 aatttcagac tgcaattgcg cgagttccgg gatatcgcga tcgcgacgta accatttaat
     1561 atctggatgg cgacgaatca cccccgttgc tgaacaaggc gcatctaata aaatgcgatc
     1621 aaactgttgc tcgccacacc attgggaagg gtaacggcca tcaccttgtt tcacggttgc
     1681 cttcatacca aggcgtttta aattgtcgta aatgcgagag aggcgctgtt cgtcaatatc
     1741 aaccgcaaca acctgcgctt ctggtgccac ctcaaggata tgcgttgttt taccgccggg
     1801 ggcggcacaa agatccaaga tgtgttcacc gttttgtggc gcaagccagg tcatgcaacc
     1861 ttgtgctgat gcatcctgaa cggtaaccca tccgtcttca aaaccaggta gcgcatgaac
     1921 aggtgcaggt gtttccagcc gtacagcatc agggtaatcc gcatgcggga aacctttcat
     1981 tcctgcttca tccagcaatg caagccagct gtcgcgggaa tgatgcgtac gattaatacg
     2041 cagccacatt ggcggacgct ggttattggc ttcggcgatg gattgccact gctctggata
     2101 cgctttttgc agacgcttca gcaaccagga aggatgcaga taacgtgcat cacgggtatt
     2161 aaactcggct aataactctt cttgccgacg ctggaactgg cgtaatacac cgttaatcaa
     2221 ccctttaagt tgcggacgct taattgcgat agcgccttca accgtttcag ccagcgcagc
     2281 atgaggtgga atgcgggtat aaagcagttg atacaaacca accataatca ggtaatgcac
     2341 agtgcgctgt ttgccggtca tcggacgggc cattaactta ttaatcagcc agtctaactg
     2401 cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga agtgctttgt ctttatcgga
     2461 aactttttgc tggagcggtg gcagaatgtt gcttaatgat tgcccttgct cgacgacttg
     2521 ttcaacggcc tgggccgcca tgctacgtaa attacgttgt tttttcataa gtataaaaat
     2581 gctcggcaag accgggctta gaagagtgga ctatcagacc agacggttgc ccggaacaaa
     2641 ccattcccga cgagagttca ggaggtcttg cgcgctcatc gctttcttac ccgcaggttg
     2701 taacgagagc aggttcagga tgccatcacc agtcgcaacc tgaatgcctt gtttgttggc
     2761 ttcaaggatc gttcctggtg cagcgttggt tgccgtatca atgaccgacg ctttccagac
     2821 tttaaccggc tgtccttcaa tttccagcca gctcattggc catgggttga aagcgcgaat
     2881 gcagcgttca agctgtgctg ccgaaagtga ccagtcaata cgcgcttctt ctttactcaa
     2941 cttctcggcg taagtgacaa gagtttcgtc ctgaacttct ggtttcgccg tgccgtctgc
     3001 caattgtttc aacgtggtga taagcccttg tgggccaagc tctgccagct tgtcgtacaa
     3061 cgtaccactg gtatcttctg cagtaatcgg gcaggagagc ttatagagca tgtcaccggt
     3121 gtctaaacca acatccattt gcataatggt tacaccagtt tctgcatcac ccgcccatag
     3181 tgagcgttgg attggtgcag caccgcgcca gcgtggcagc agtgaaccat gaatgttgat
     3241 acagccaaga cgcggcatct ccagcacagc tttaggcaga attaaaccat aggcgacgac
     3301 gaccataaca tcagcctgca aatcggcgac cagttgctgg ttttcttgtg gacgcaggga
     3361 aacaggttga aaaacgggca gaccttcttc ctcagccaga actttaatcg ggctgggcat
     3421 cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg aacacgccaa cgacgttatg
     3481 accagaagac aacagcgcgt cgagatgacg cgctgcaaag tcaggtgtac ccgcaaaaat
     3541 aatacgtagt gattctgaca cgttagttct tatccttaag cccgggcttt cagacgatcc
     3601 agtttttcaa ctttctgacg aattcgttgt tgtttcagcg gtgacagata atccataaac
     3661 agtttgccaa ccaggtgatc catctcatgc tgaatacaga tagctaacag accctctgct
     3721 tccagttcaa atggtttacc gtcgcggtca agggcgcgaa ttttaacttt ctctgcgcgc
     3781 ggcactaaag cacgttgttc agggatcgac aggcaacctt cttcaatgcc tgtttcgccg
     3841 cttttttcta aaagctccgg attgattaac accagccgtt cgtcacggtt ttccgaaaca
     3901 tcaataacaa tgatacgttg atggatatca acctgggttg ccgccaggcc aataccttct
     3961 tctgcgtaca tcgtctcgaa catatcatcg acgatacgct gaatttctgc attcacttct
     4021 tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa tatgtaacac ttgcaaaact
     4081 gacataaatc tccagagatg tgttcaggag ttagaaagat tatttcttct attctagaca
     4141 aatccccctc tgattgacag catcacttga ttgacagcat cacttgattg acagcatcac
     4201 tgaccaatcg caaagattgc taaggcttgc ttatggcagg gagataagga tggtcgatat
     4261 agaaatttgg ctgcgtttaa tgagcatcag cagcttgtac ggcgatgata tggtccgtat
     4321 cgctcactgg ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc aaacagggct
     4381 tacattgcgg caggcacaac gctttctttc atttccacga aagagtatcg aaagctcact
     4441 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
     4501 tcaacttcag gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
     4561 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
     4621 atgggggcga ttattttgcg aaactctggc gacgtgtgga gtgacaatta cgagtggact
     4681 ggcgcgtgga atcgatggtg tggtgcataa agcggcctta caggtaaatg gcgtcagcat
     4741 tgctgtattg gggaatggac ttaataccat tcatccgcgt cgccatgccc gactggctgc
     4801 cagtctgttt gaacagggtg gcgctctcgt ctcggaattt cccctcgatg ttccacccct
     4861 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
     4921 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
     4981 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
     5041 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
     5101 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
     5161 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
     5221 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
     5281 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
     5341 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacact gaagctgagt
     5401 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggtttt gagcgagaag
     5461 atatctacaa tgccctgcta tggctggaaa aacttgctga ttatcaggaa gggttggcag
     5521 aaccgatgca actggcctcg gatcctctct ccatgcgtat ttatacaccg gaagagtgtg
     5581 aacgactgga tgccagctgc cgtgggtttc tgcttttcct tgagcagatt caggtgctca
     5641 accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg
     5701 aactggatga tctgaaatgg gtgatcctga tggtgttgtt caacatcccg ggctgcgaaa
     5761 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
     5821 catttcttta attcagcata agttgttatg gcaaaatcag cactgttcac ggtgcgtaat
     5881 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
     5941 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
     6001 gatggacata ttgtcaaagt tctggagggg caggtttgcc ccgtatgtgg cgcaaatctg
     6061 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
     6121 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgtcggacg
     6181 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
     6241 ccggagtgtc aatttgccat taacttcaaa ccaatagctg gagaatgccc tgagtgtcat
     6301 tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa
     6361 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
     6421 tatcgcagct gcgatagatg tcctcaatga aaaacgtgtc atcgcctatc caacggaagc
     6481 cgttttcggt gtcgggtgcg atcctgatag cgaaacagca gtgatgagac tgttggagtt
     6541 aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa
     6601 accctatatt gatgacacca tgttgactga cgtgcagcgt gaaaccattt tttcctgctg
     6661 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
     6721 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
     6781 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
     6841 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
     6901 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
     6961 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
     7021 cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt
     7081 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag
     7141 gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg
     7201 aacgggcagc gttggctggt gcagttaata ccctcaagcg gttagaagat ggacgcctgc
     7261 tgggtgacaa taccgatggt gtaggcttgt taagcgatct ggaacgtctg tcttttatcc
     7321 gccctggttt acgtattctg cttatcggcg ctggtggagc atctcgcggc gtactactgc
     7381 cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
     7441 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
     7501 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
     7561 cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga
     7621 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
     7681 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
     7741 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
     7801 ccagtttccg gacagggaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
     7861 cgtgaatggt atgcaactga catgcgcgat ctctggcgag agtctggcgt atcgctttac
     7921 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg acctggaaga
     7981 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
     8041 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
     8101 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
     8161 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
     8221 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
     8281 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
     8341 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
     8401 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
     8461 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
     8521 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
     8581 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
     8641 tagtatgcaa aaaagcaccg ttttgtgtgc gattgcagca aaaagggtga aaaaacaaca
     8701 aacagaaaag aagatcaaaa aaatacttgt gcaaaatatt gggatcccta taatgcgcct
     8761 ccgttgagac gacaacgtga aacacttcac aggatggtcg gaacaacgaa gag
//
LOCUS       DAJTRM010000144         8666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRM010000144 DAJTRM010000000
VERSION     DAJTRM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.144"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(98..718)
                     /gene="entD"
                     /locus_tag="OO322_003732"
     CDS             complement(98..718)
                     /gene="entD"
                     /locus_tag="OO322_003732"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5908438.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(893..3133)
                     /gene="fepA"
                     /locus_tag="OO322_003733"
     CDS             complement(893..3133)
                     /gene="fepA"
                     /locus_tag="OO322_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5908439.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3376..4578
                     /gene="fes"
                     /locus_tag="OO322_003734"
     CDS             3376..4578
                     /gene="fes"
                     /locus_tag="OO322_003734"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5908440.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4581..4799
                     /gene="ybdZ"
                     /locus_tag="OO322_003735"
     CDS             4581..4799
                     /gene="ybdZ"
                     /locus_tag="OO322_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5908441.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4796..8641
                     /gene="entF"
                     /locus_tag="OO322_003736"
     CDS             4796..8641
                     /gene="entF"
                     /locus_tag="OO322_003736"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5908442.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         1873 a         2318 c         2387 g         2088 t
ORIGIN      
        1 catatccggt tgtcaggtca gagtggtaag gcacctgctt tacactttcg cccgcagtca
       61 gtgatggctg cgggcgaatc gtaccagatg ttgtcaatta atcgtgttgg cacagagtta
      121 tgactatttt ttcttttatc tgccagtgca cagcaaacat ctcattctca cgatgaatga
      181 tgacctgctg tttattccag ctaattatct gatagtccag aaaacctgca tcagtttgga
      241 tctcacttgc cttaaatgcg ctctctttgg cggaaaatgc aagtgtcagc gccagagaaa
      301 aggctaaacc gcagtctgcg agtcgctcgt gttctgctgg tgtaataatg ttgtctgtca
      361 attcggtcgc tgtttgcgca gaaaaaattt cttcgatatc aacgccaatc ggttgacggg
      421 aaaccacggc caatgccgta gccccgcagt ggctaatact gccgtatacc tccgcaggcc
      481 acaccggttg gcgtagctcg ccgattgcgg gcacacattt atagccatat tcccgcaagg
      541 cataaacagc agcgatccgt ccggctaaat gctctgtttt acgtttacgt ccagcgtgtt
      601 gcagttgtgc gtagtgcggc agccagagta aatcctgctc acaaaaattc gccggatcga
      661 actcaacaaa atgcagcgta tgtccggcaa aggggaggga ggtatgcgta gttttcatat
      721 cgaccatatt cgagactgat gacaaacgca aaactgcctg atgcgctacg cttatcaggc
      781 ctacgtgatc ctgcaatata ttgaattggc aagatctttg taggccggat aaggcgttca
      841 cgccgcatcc ggcgtgaacg acgcgcactt tgtcaacaat ctgacgttag catcagaagt
      901 gagtattaat gctcatatac cacgtacgtc ccggctcgtt ataggtatac gcgccggcac
      961 cggcgatata gttggctcct gccagatcgc ccgtggtctg ggcattacct gcacgccaca
     1021 aacgtttgtc gaacaggttg tccacgccgc cggtcagact gacattcttc gtcacatccc
     1081 aggtcgcgct cagacccaca atgctgtaag gactgatttc tttggtttcc gggccaaccg
     1141 ctggctgacc tttatagttg tacttcttcg gctgctgctt gccgtaccag gtgaaggtcg
     1201 tttgcatcga caaatcttcc cgtgcctgcc agctcagcgt tgagttcaac gtgtactccg
     1261 ggatgatcga caaacggtcg cccgtggttt tgttttcact cttcagcata taagtgatgt
     1321 tattggtcca catcaccgtt tcgctaactg gtacgtttaa cgatccttcc agaccttcaa
     1381 ccactgcttt cggcacgtta tcccactgat agagatcagt gccgactgcg ttttgcccta
     1441 cagccacata gcccgcttca atcttattgc gataatcgtt acggaaccag gtgacgcccg
     1501 ccagccagcc gtcgcgtttg aactccagac caatctcttt gttgatgctg gtttctactt
     1561 tcaggtcatc gttaccttgc agatagcagc cgcccgcgct ggcatagcag ccctgacctt
     1621 tactgtagag aatgtagttc gggttagtct ggtataggct cggcgcttta taggcgcggg
     1681 caatgcctat tttcagcgtg aagtcatcgc ctaaaccttg cgatatgttc agcgccgggc
     1741 tccagttatt gccgacaata ctgtgatgat cgaaacgcag ccccggcgtt acgatggtgc
     1801 tgtcagtcag ctccatgttg ttttcggcaa acagcgagaa aatttctgct tttgaatacg
     1861 ggctacggtc ggtggcactc acgccatcaa tagcgccacc ggtattcgtt ccggtcagtg
     1921 cttgggtgtt ggaacttaag tccttcatcc gttgctgatt ccactccgta cccagcgtca
     1981 gcgtctggtt aacgaggaaa tcaatcggca ggttaacttc gctgtgcagc atcacgtcat
     2041 cgagatcgat atcgacgaaa tcctgtgtcg ctttttcgtt aaatttccct tcggtaccgc
     2101 ccgccagacc ttccggaata cgcgagttac gggtgtgttc atactgcacc cagttgctgg
     2161 tggtcacgcc gttatcccag ccaccgttcc aggtcagcgc gtagttctgg cgatacaggc
     2221 ggttggtttc atcgccatat ttcgagcgag tgtaagcgtc agagttggtg ttctgggtat
     2281 cgcccgcata caggttaccc tggcggctgt aacccgcttc cagttccagc gattgcaatg
     2341 gtgcgaagtc ccagcgcacc acgccgttaa tatctttatt gatcacccct tcgcgcccgg
     2401 ctggtaacgt cgtggcatac gttccggcac gcgcggactg atggccctgg ttgatatccc
     2461 acgcgtcagc ctgagttttg tcgaggttgc catacaagcg gaagctgaat tcgtcgccca
     2521 gcggaccggt caggctaaag ttagtgcgtt tggtggcacc ttcctcttta tgttctggcg
     2581 cattgaaata tgcgtcccag gagccgtgcc actcgccgct gccttttttg gtaatgatgt
     2641 taaccacgcc gcccgccgcg ccgttgccat aacgcgcagc tgccggacca cgcagaactt
     2701 caatacgttc aatcatttca ggtggcaccc aggaggtatc accacgggta tcgcgctcgc
     2761 cacgccagcc ctgacgcacc gagttacggc tgcttaccgg cttgccgtca atcaaaatca
     2821 gcgtgttttc cggacccata ccgcgaatat caatctgtcg gttattccca cgctgaccac
     2881 tggtggagtt accggtcagg ttaacgcctg gcatggtacg gatgatctcc gacacatcgc
     2941 gggcaaccgg gtttttgcgg atttcatctg cggtgatggt cgaaacgcca ggagcctgca
     3001 agttctgctc ggcggcggta acgacaatgg tatcgtcatg tgaaacaggt gtatcggtcg
     3061 gctcttgtgc ctgcgctacc ccataaatcc ccagattgac caacaaggcc agggaatgaa
     3121 tcttcttgtt cattgtttta ttcctgcatt tttgccacga attgcaactg tcgggcatgg
     3181 tcgtcatcaa cacgacgcat cccgttaccg cgaaaacctt tgatcctgaa agacacgcag
     3241 tgcagttggt taattaatgt ccgcgcttcc cacggcgcgc cattacgcta ttgcaaatgc
     3301 aaatagttat taataacatt atcaatatat ttctgcaatc aatgaaaaat tgcacagtaa
     3361 acatggggtt atggtgtgac ggcgttaaaa gtaggaagtg agagctggtg gcagtcgaaa
     3421 catggcccgg aatggcagcg tctgaatgac gaaatgtttg aggtcacttt ctggtggcgt
     3481 gatccccaag gttctgaaga atactcgacg ataaagcgcg tatgggtcta catcactggt
     3541 gtgaccgatc atcatcagaa cagccagccc cagtcgatgc agcgaattgc aggtactgat
     3601 gtctggcagt ggacgacaca actcaatgcc aactggcgcg gcagctactg ctttattccc
     3661 accgaacgcg atgacatttt ttctgcacca tcccccgatc gcctcgaatt acgtgaaggc
     3721 tggcgaaaac tattacccca ggcaatagcc gatccgctga atccacaaag ctggaaaggc
     3781 gggctagggc acgctgtttc tgcactcgaa atgccgcaag cgcctctgca accgggatgg
     3841 gattgtccgc aagcgccaga aataccagcc aaagaaatta tctggaaaag tgaacggttg
     3901 aaaaattcac gtcgtgtatg gatttttacc accggcgatg taacagcaga agaacgcccg
     3961 ctggcagttt tgctcgatgg cgaattttgg gcgcaaagta tgcccgtctg gcctgtgctg
     4021 acttcgctga cccatcgtca gcaacttcct cccgccgtgt atgtgttgat cgacgctatc
     4081 gacaccacgc accgcgccca cgaactgccg tgtaatgcgg atttctggct ggcagtacag
     4141 caagagttat tacccctggt gaaagttatt gcccctttta gcgatcgcgc cgatcgcacc
     4201 gtggtcgccg ggcagagttt tggtgggctg tccgcgctgt atgccggact gcactggcct
     4261 gaacgctttg gctgtgtatt aagccagtca ggatcgtact ggtggccgca tcggggcggg
     4321 cagcaagagg gcgtgttact tgaaaagcta aaagctggtg aagttagcgc cgaaggtctg
     4381 cgcattgtgc tggaagcggg tattcgcgag ccgatgatca tgcgggccaa tcaggcgctg
     4441 tatgcgcaat tacaccctat aaaagaatcc attttctggc gtcaggttga cggcggacat
     4501 gatgcgcttt gttggcgcgg tggcttgatg caggggttaa tcgacctctg gcaaccactt
     4561 ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatcca cagggagcgt
     4621 tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac
     4681 cggcaggctg ggacattgtg tgtcagccgc agtcacaaga gtcctgccag cagtggcttg
     4741 aagcccactg gcgtactctg acaccggcga atttcaccca gttgcaggag gcacaatgag
     4801 ccagcattta cctttggtcg ccgcacagcc cggcatctgg atggcagaaa aactgtcgga
     4861 tttaccctcc gcctggagcg tggcgcatta cgttgagtta accggagagg ttgatgcgcc
     4921 attactggcc cgcgcggtgg ttgccggatt agcgcaagca gatactttgc ggatgcgttt
     4981 tacggaagat aacggcgaag tctggcaatg ggtcgatgat gcgctgatat tcgaactgcc
     5041 agaaattatc gacctgcgaa ccaatattga tccgcacggt actgcgcagg cattaatgct
     5101 ggcggatttg caacaagatt tgcgcgtcga tagcggtaaa ccactggtct ttcatcaact
     5161 gatacaggtg gcagataacc gctggtactg gtatcagcgt tatcaccatt tactggtcga
     5221 tggcttcagt tttccggcca ttacccgcca gatcgccaac atttactgcg cattgctgcg
     5281 tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca
     5341 gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggtgg aacagcgtcg
     5401 ccaactgcca ccgcccgcgt cactttctcc ggcaccttta gcggggcgca gcgcctcggc
     5461 agatattttg cgtctgaaaa tggaatttac cgacggggaa ttccgccagc tggctacgca
     5521 actttcaggt gtgcagcgta ccgatttagc ccttgcgctg acggctttgt ggctggggcg
     5581 attgtgcaat cgcatggact acgccgccgg atttatcttt atgcgtcgac tgggctcggc
     5641 ggcgctgacg gctaccggac ccgtgctcaa cgttttgccg ctgggtattc atattgcggc
     5701 gcaagaaacg ctgccggaac tggcaacccg actggcaacc cgactggcag cacaactgaa
     5761 aaaaatgcgt cgtcatcaac gttacgatgc cgaacaaatt gtccgtgaca gcgggcgagc
     5821 ggcaggtgat gaaccgctgt ttggtccggt actcaatatt aaggtatttg attaccaact
     5881 ggatattcct ggtgttcagg cgcaaaccca taccctggca accggtccgg ttaatgacct
     5941 tgaactggcc ctgttcccgg atgaacacgg tgatttgagt attgagatcc tcgccaataa
     6001 acagcgttac gatgagccaa cgttaatcca gcatgctgaa cgcctgaaaa tgctgattgc
     6061 ccagttcgcc gcggatccgg cgctgttgtg cggcgatgtc gatattatgc tgccaggtga
     6121 gtatgcgcag ctggcgcaga tcaacgccac tcaggttgag attccagaaa ccacgctgag
     6181 cgcgctggtg gcagaacaag cggcaaaaac accggatgct ccggcgctgg cagatgcgcg
     6241 ttacctgttc agctatcggg aaatgcacga gcaggtggtg gcgctggcga atctgctgcg
     6301 tgagcgcggc gttaaaccag gggacagcgt ggcggtggca ctaccgcgct cggtcttttt
     6361 gaccctggca ctccatgcaa tagttgaagc tggcgcggcc tggctaccgc tggataccgg
     6421 ctatccggac gatcgcctga aaatgatgct ggaagatgcg cgtccgtcgc tgttaattac
     6481 caccgacgat caactgccgc gctttagcga tgttcccaat ttaaccaacc tttgctataa
     6541 cgccccgctt accccccagg gcagtgcgcc gctgcaactt tcacaaccgc accacacggc
     6601 ttatatcatc tttacctctg gctccaccgg caggccgaaa ggggtaatgg tcgggcagac
     6661 ggctatcgtc aaccgcctgt tgtggattca aaatcattat ccgctcacag gcgaagatgt
     6721 cgttgcccaa aaaacgccgt gcagttttga tgtctcggtg tgggagtttt tctggccgtt
     6781 tattgccggg gctaaactgg tgatggctga accggaagcg caccgcgacc cgctcgctat
     6841 gcagcaattc tttgccgaat atggcgtaac gaccacgcac tttgtgccgt cgatgctggc
     6901 agcatttgtt gcctcgctga ctccgcaaac cgctcgccag agttgcgtga cgttgaaaca
     6961 ggttttctgt agtggtgagg ccttaccggc tgatttatgc cgcgaatggc aacagttaac
     7021 cggcgcgccg ttgcataacc tgtatggccc gacggaagcg gcggtagatg tcagctggta
     7081 tccggctttt ggcgaggaac tggcacaggt gcgcggcagc agtgtgccga ttggttatcc
     7141 ggtatggaat acgggtctgc gtattcttga tgcgatgatg catccggtgc cgccgggtgt
     7201 ggcgggtgat ctctatctca ccggtattca actggcgcag gggtatcttg gacgacccga
     7261 tctgaccgcc agccgcttta ttgccgatcc ttttgctcct ggtgaacgga tgtatcgtac
     7321 cggagacgtt gcccgctggc tggataacgg cgcggtggag tacctcgggc gcagtgacga
     7381 tcagctaaaa attcgcgggc agcgtatcga actgggcgaa atcgatcgcg tgatgcaggc
     7441 gctgccggat gtcaaacaag ccgttaccca cgcctgtgtg attaaccagg cggcagccac
     7501 tggtggtgat gcgcgtcagt tggtgggcta tctggtatcg caatcgggcc tgccgttgga
     7561 taccagcgca ttgcaggcgc agcttcgcga aacattgccg ccgcatatgg tgccagtcgt
     7621 tctgctgcaa ctgccgcagt tgccacttag cgccaacggc aagctggatc gcaaagcctt
     7681 accgttgcct gaactgaagg cacaagcgcc agggcgtgcg ccgaaagcgg gcagtgaaac
     7741 gattatcgcc gcggcattcg cgtcgttgct gggttgtgac gtgcaggatg ccgatgctga
     7801 tttctttgcg ctgggcggtc attcgctact ggcaatgaaa ctggcagcgc agttaagtcg
     7861 acagtttgct cgtcaggtga cgccggggca ggtgatggtc gcgtcaaccg tcgccaaact
     7921 ggcaacgatt attgatggtg aagaggacag ctcccggcgc atgggattcg aaaccattct
     7981 gccgttgcgt gaaggtaatg gcccgacgct gttttgtttc catccggcgt ccggttttgc
     8041 ctggcagttt agcgtgctct cgcgttatct cgatccacaa tggtcgatta ttggcattca
     8101 gtcgccgcgc cctcatggac ccatgcagac agcgacaaac ctggatgaag tctgcgaagc
     8161 gcatctggca acgctacttg agcaacaacc gcacggcatt gcggcgcggc tgcgtgcccg
     8221 tggcgaacag gtggcatttc ttggtttgct ggatacctgg ccgccagaga cgcaaaactg
     8281 gcaggaaaaa gaggctaatg gtctggaccc ggaagtgctg gcggagatta accgcgaacg
     8341 cgaggccttc ctggcggcac agcagggaag tacttcaacg gagttgttta ccaccattga
     8401 aggcaactac gctgatgctg tgcgcctgct gacgactgct catagcgtac cgtttgatgg
     8461 taaagcgacg ctgtttgttg ctgaacgcac gcttcaggaa ggtatgagcc ccgaacgcgc
     8521 ctggtcgccg tggattgcgg agctggatat ctatcgtcag gattgtgcgc atgtggatat
     8581 tatctctcca ggggcgtttg taaaaattgg gccgattatt cgcgcaacgc taaacaggta
     8641 aattaatatt atctataaac ccataa
//
LOCUS       DAJTRM010000145         8535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRM010000145 DAJTRM010000000
VERSION     DAJTRM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.145"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..137)
                     /locus_tag="OO322_003737"
     CDS             complement(<1..137)
                     /locus_tag="OO322_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908443.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRLFLPEGTLGQW
                     VT"
     gene            182..361
                     /locus_tag="OO322_003738"
                     /pseudo
     CDS             182..361
                     /locus_tag="OO322_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(370..768)
                     /gene="vapC"
                     /locus_tag="OO322_003739"
     CDS             complement(370..768)
                     /gene="vapC"
                     /locus_tag="OO322_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5908444.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(768..995)
                     /gene="vapB"
                     /locus_tag="OO322_003740"
     CDS             complement(768..995)
                     /gene="vapB"
                     /locus_tag="OO322_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5908445.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1077..1448
                     /locus_tag="OO322_003741"
     CDS             1077..1448
                     /locus_tag="OO322_003741"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5908446.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1469..6330
                     /gene="traI"
                     /locus_tag="OO322_003742"
                     /pseudo
     CDS             1469..6330
                     /gene="traI"
                     /locus_tag="OO322_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6350..7096
                     /gene="traX"
                     /locus_tag="OO322_003743"
     CDS             6350..7096
                     /gene="traX"
                     /locus_tag="OO322_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5908447.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7151..7711
                     /locus_tag="OO322_003744"
     CDS             7151..7711
                     /locus_tag="OO322_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5908448.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7842..8054
                     /locus_tag="OO322_003745"
     CDS             7842..8054
                     /locus_tag="OO322_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5908449.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8298..>8535
                     /locus_tag="OO322_003746"
     CDS             8298..>8535
                     /locus_tag="OO322_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5908450.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1962 a         2131 c         2715 g         1727 t
ORIGIN      
        1 gtaacccatt gtcctaaagt gccttcagga aggaataatc gggaagcgtc ttcactgatc
       61 gaaagttgat tttcaagaac cgttctgaca gcttcggctt tgaactcttt agagtaacgt
      121 cgggtttttc tgctcattat tagctccttc tgatgccatt ctatttcagg aaggagtgtc
      181 cgttaaactc aggctacctc agatcagtga atccggtgaa gtggtggata tggccgctta
      241 tgaggcatgg cagcaggaaa atcatccgga cacctggcag cagatgcagc gtcgtgaaga
      301 ggtgaacatc aatgtgcacc gggaacgcgg ggaggatgtt gagccgggag atgatttctg
      361 atgaacaggt cagctccagt cttcagttct caggccgccc acacgttcaa attcccgggt
      421 gttattagtc acaataatca gcccccgact gcgggcatga cctgcgatca tttgatcaaa
      481 tggcccgaca gggcgtccct gacgggcaag ttctgctctt atctggccgg tgtgtgtggc
      541 tgcagcagcg tcgtaatcca gaacatcaat gcgggaaaca aatccctcga tcacagcgag
      601 attacgttca ggcatctggc ttttttctgc accatatatc agctccatca gggtgaccga
      661 actgatacac attttcccct ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt
      721 ctttatcgta aaaatgcaga tgttggtatc gagcataaac ttcagcatca gaatgactcc
      781 ctttcttgca tgccgggctg ttccctgtta tccataaaat cggtgctgac gctgtgtccg
      841 tcgaaccatt cgtcccatgt ctctccggct ggcgtaatga ttctggttcg tccgacagca
      901 atcacttcaa cgcgttttac gttttccggc aatgcaaccg cttttggcag tctgaccgcc
      961 tggctgcggt tgctgagaaa tacggtggtt tccattattt gcctccttat gtggatatgt
     1021 cttgtgtata tctattgtgg gattgatacc gggatatgtc aaagggatat acgtttatga
     1081 tgagtatcgc gcaggtcaga tcggccggaa gtgccggtaa ttattatacc gacaaggata
     1141 attactatgt gctgggcagc atgggagaac gctgggccgg ccagggggct gaacaactgg
     1201 ggctgcaggg cagtgtcgat aaggatgtct ttacccgcct tctggagggc aggctgccgg
     1261 acggggcgga tttaagccgc atgcaggatg gcagtaacag gcatcgtccc ggctacgacc
     1321 tgaccttctc cgctcccaaa agtatctcca tgatggccat gctcgggggg gacaaacgtc
     1381 ttattgaagc acataaccag gccgtggatt ttgctgtccg tcaggtggag gccagtgcct
     1441 ccacctgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
     1501 taaccacgac accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa
     1561 tgttacgcag tataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg
     1621 gttcattgag aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact
     1681 gaaagagcag gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg
     1741 ggaaatgccg ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc
     1801 cgtcggggaa gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc
     1861 caaacagcac gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac
     1921 cgggttcgac atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca
     1981 gacacccgga cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat
     2041 tgccggatta agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt
     2101 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     2161 catcagccgt gagcagctta tccccctcga tcgtgagaag gggctgttca cgttcgggat
     2221 tcatatgctc gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg
     2281 ggtgaccgta catccggaga aaagtgtccc ccggacggcc ggttacagcg atgccgtcag
     2341 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     2401 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca
     2461 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     2521 tgaactgata accggacgtc gtcagttgct ggaaggaatg gccttcacgc cgggcagtac
     2581 cgttatcgtc gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     2641 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     2701 cagtgcactg atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga
     2761 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtcggctggc cggagatttt
     2821 gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt acgggaacag
     2881 gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct cggacacccg
     2941 gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg ttatctgcgg
     3001 gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg cagtcatgac
     3061 cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg gaatgcgcag
     3121 ggcgaaacgc aggtggtgcg tatttcttcc ctggacagca gctggtcgct gttccggccg
     3181 gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat tcccggactc
     3241 cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc gatgacggtt
     3301 gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc gttcactgca
     3361 ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga cagtgcgacg
     3421 gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaatgg tctggcccgc
     3481 agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc ggaaaaactt
     3541 gcccgccatc cctcctttac ggtggtttct gagcagataa aggcgcgtgc cggtgagaca
     3601 ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc acagcaggcc
     3661 attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat ggtggacctg
     3721 ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct gggaggggaa
     3781 atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa aggctatggc
     3841 acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct tcgccatatt
     3901 ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga actcatggag
     3961 aaactgacat caggacagcg cgccgccacc cgtatgatac tggaaacgtc tgaccgtttc
     4021 acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc ggtgatgtca
     4081 gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg gcccacacac
     4141 cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc gtcctttctg
     4201 catgacacgc agctgcagca gcgcagcgga gaaactccgg atttcagcaa cacgctgttc
     4261 cttctcgatg agagctctat ggtgggcaat accgacatgg cacgggcata cgccctgatt
     4321 gcggccggtg gcggtcgggc tgtggccagc ggtgacacgg accagctgca ggccatcgcg
     4381 cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggt catcatgaag
     4441 gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat taaccgggat
     4501 gtggaaaggg cgctgtccgg gcttgagcgt gtgaaaccgt ctcaggtgcc acgtctggag
     4561 ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga agcgaaactg
     4621 gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt ttccggatgt ccccatgaca
     4681 ctgtatgaag ccattgtccg cgactatacc ggcaggacac cggaagcacg ggagcagacg
     4741 ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat tcatgatgca
     4801 cgggaaaagg ccggagaact ggggaaggtg caggtcatgg tgcctgtcct gaacacagcg
     4861 aatatacgcg acggggagct gcgtcgtctc tccacctggg agaataaccc ggatgccctt
     4921 gccctggtgg ataatgtgta tcaccggatt gccggtatca gtaaggatga cgggctgata
     4981 accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc ggtggctgaa
     5041 ggcgtcacgc tgtacacccc ggacaccatc cgggtgggga ccggtgacag gatacgcttc
     5101 acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt gacagcggtt
     5161 tccggtgaca gtgtcacact ttctgacggt cagcagaccc gtgtgattcg ccccggtcag
     5221 gagcgggcag agcaacatat tgacctggcc tatgccatca ccgcccacgg tgcacagggg
     5281 gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa actgatggcc
     5341 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
     5401 gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac
     5461 gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg
     5521 gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc
     5581 gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg
     5641 gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc
     5701 acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg
     5761 gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat
     5821 atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagaatc
     5881 gccggtgaag gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt gtggggggat
     5941 atcccggaca acagcgtcca gccgggagcc ggaaatggtg aaccggtcac ggcagaggta
     6001 ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa
     6061 attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagcaggct
     6121 gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg ggaagccgcg
     6181 ctgccggaga gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg ggaagttgcc
     6241 cgggaaaatt tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg
     6301 cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga tgacaacgga
     6361 taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc
     6421 atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
     6481 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
     6541 gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
     6601 tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc agttcgcata
     6661 ttatctggca ggctttcctt ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
     6721 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccatactcct
     6781 gatggccctg tgggggcctt tgtccggcac cagttacggt attgccgggt tgctgatgct
     6841 ggcagtcagc aaccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc
     6901 ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc
     6961 cggtcttgtg atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa aatcattacc
     7021 ccgtttctgg cccggagact ttttcccgac gttctatgcc tgtcatctgg ctgtgctggg
     7081 cgttctggcg ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt cgtcagaggc
     7141 agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga agacagaggg
     7201 aacagcgcct gtccgcagcc ggaaaaccat catcaatgtc accacgccac caaagtggaa
     7261 ggtgaaaaag cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg cggcaaaaaa
     7321 agcgcaggcc agacaggcgc tgtccattta tctgaacctg cccacgctgg atgaggctgt
     7381 gaataccctg aagccctggt ggccgggatt atttgacggt gacacgcccc ggcttctggc
     7441 ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc cgctctcgca
     7501 taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc tgtgtgccat
     7561 gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata tttctcagga
     7621 ggaggaagcg tatgcgggag cgcgtctggc gaaaattcgt cgccagaacc ggataaaggc
     7681 agaacttcag gccgtgcttg atgagaaata aaaaaagcct cccccagaga gaggaggcag
     7741 ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt ccactgaatt
     7801 atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga ttcagaaaca
     7861 gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc gttattctgg
     7921 aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc tgtgttgcgc
     7981 ctaggacaga ctggcagcag tttgcccgga aaagggctga acactgccac cggcgctgcc
     8041 ggggaagggt gtaacgaaaa aagcagcctt cattactcta ggagtcagaa ggctgaaagg
     8101 agtttatgag atttgcttct taatatccgg tcgggcagta tgaccggaca gttctgagta
     8161 gcacagagtc ttttccgcat taagtcgatt ttttatattt tgtgattagt ttcacaaaat
     8221 aaggtgttat tcaatgtgtg ctgcactatt caggggggct gaaatacagg tggcatttat
     8281 ctgatatgga gaataacatg aggaaatata ttccactggc attatttatc ttttcatggc
     8341 cggtattgtc tgcagatatt catggacggg ttgttcgtgt actggacggt gataccatcg
     8401 aggtcatgga ctcactgaag gcggtgcgga tcaggctggt aaatattgat gcaccggaaa
     8461 agaaacagga ttatggccgg tggtcaacag acatgatgaa atcactggtc gcaggaaaaa
     8521 cggttaccgt cacat
//
LOCUS       DAJTRM010000146         8494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRM010000146 DAJTRM010000000
VERSION     DAJTRM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.146"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(765..1961)
                     /gene="hemY"
                     /locus_tag="OO322_003747"
     CDS             complement(765..1961)
                     /gene="hemY"
                     /locus_tag="OO322_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5908451.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1964..3163)
                     /gene="hemX"
                     /locus_tag="OO322_003748"
     CDS             complement(1964..3163)
                     /gene="hemX"
                     /locus_tag="OO322_003748"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5908452.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(3185..3925)
                     /gene="hemD"
                     /locus_tag="OO322_003749"
     CDS             complement(3185..3925)
                     /gene="hemD"
                     /locus_tag="OO322_003749"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5908453.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3922..4863)
                     /gene="hemC"
                     /locus_tag="OO322_003750"
     CDS             complement(3922..4863)
                     /gene="hemC"
                     /locus_tag="OO322_003750"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5908454.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5250..7796
                     /gene="cyaA"
                     /locus_tag="OO322_003751"
     CDS             5250..7796
                     /gene="cyaA"
                     /locus_tag="OO322_003751"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5908455.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7836..8156)
                     /gene="cyaY"
                     /locus_tag="OO322_003752"
     CDS             complement(7836..8156)
                     /gene="cyaY"
                     /locus_tag="OO322_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5908456.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8204..>8494)
                     /locus_tag="OO322_003753"
     CDS             complement(8204..>8494)
                     /locus_tag="OO322_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5908457.1"
                     /translation="FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTW
                     TDTEVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1928 a         2205 c         2188 g         2173 t
ORIGIN      
        1 gacggggtat ctgctgcgct ggccatgaga ggaagagcgg cggcgacagc aacaacaaga
       61 cgtttgggtg aaaacgaaaa ttccatgcaa aatgctccgg tttcatgtca tcaaaatgat
      121 gacgtaatta agcattgata attgagatcc ctctccctga caggatgatt acataaataa
      181 tagtgacaaa aataaattat ttatttatcc agaaaatgaa ttggaaaatc aggagagcgt
      241 tttcaatcct acctctggcg cagttgatat gtaaggcagg tttattatat cgcgttgatt
      301 attgatgctg tttttagttt taacggcaat taatatatgt gttattaatt gaatgaattt
      361 tatcattcat aataagtatg tgtaggatca agctcaggtt aaatattcac tcaggaagtt
      421 attactcagg aagcaaagag gattacagaa ttatctcata acaagtgtta agggatgtta
      481 tttcccgatt ctctgtggca taataaacga gtagatgctc attccatctc ttatgttcgc
      541 cttagtgcct cataaactcc ggaatgacgc agagccgttt acggtgctta tcgtccactg
      601 acagatgtcg cttatgcctc atcagacacc atggacacaa cgttgagtga agcacccact
      661 tgttgtcata cagacctgtt ttaacgcctg ctccgtaata agagcaggcg tttttttatg
      721 catcagaaag gtcccggagg tgctttcctc cgggtgagaa ggaactactg tggcgggtta
      781 ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg cagcttcttc cggcttgtgc
      841 agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg cgtccggacg ttgtttcagc
      901 gccgcgcgga aggcgagcga tgcttcctgc cattcaccgt gcttcatcag tgactgaccc
      961 aatgtgctcc acaacagcgg gcgatcaccg acgtttttga tttgctggcg tagcactttt
     1021 tccagctgtt ccgggttatt ggttttcaga cgcgggatcg gcagcagtag gcgatcgtca
     1081 tactggcgtt tcaggccgtc gatgataatt tgctgggcag tatcatgatc gtcacattca
     1141 ataagatgtt ccgccattgc cacctgcaac gctacctgat gacgcgtttt ccggctttgg
     1201 tttttccacc agttacgcaa accttcacta ccgttatcgg cacgcgcctg atccatcagg
     1261 ccaatccatg cctgttgttc cagcattgca cgatgttctt catcaccaac atgggctttc
     1321 gccattgatg ggatgatatc cagcagagaa ctccatgccc ctgtgcggat atacgcctgt
     1381 tccgccagac gtaatacttc aggatggcgt ggcgtaactt ccagcagctt atccacgccg
     1441 tggcgtgcag catggttttc attacgggcc agttgcagac gtacacgggt gatttctacc
     1501 ggaatggtgt cattgccggc cagctccgct gcgcgttcca gatgttggtt ggcgcgtgct
     1561 tcatcaccac gttgttgcgc ggcttcggca gccagtagat agttcaccac cggttgttca
     1621 gcgtgatcgg catttttcgc catcagcttt tcaacttgct gataatcacc ttccgccagt
     1681 ttcagcagcg cctgttcggt ctgcttacgc gcacggcgac gcttacgtcc gacaaaccat
     1741 ccacgggtgt gcgcgccagt gcggaagatc cgccgcagta gccactcaat ggcaaacagc
     1801 actaccatcg ccagaatcaa tatgatcgcc aggcccgtga cgctggtttc gatattgtag
     1861 ttgtcggtct ggatcagcac ataaccctga tgaccggcaa tcatcgggcc aaccactatc
     1921 cccgcaatca gcaaaacaaa gagtaataac acttttagca tggttattct ccttgcggcg
     1981 cggctgccgg agtatcacct tgcggtgcag gtgcaggtgc aggtgcaggt gcaggtttag
     2041 cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg cacgcgagtc tgcatcagtt
     2101 tttccagcat cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc
     2161 ttaactggtc cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag
     2221 cacgtaccca ggtggagacg ttctccagcg cctggcgata agtctcttcc tgatgacgcg
     2281 gtacagcttg tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct
     2341 gatttggcgc taacagcggt acggcggtgt catcacgacg gcgaatcgta atgaagttgt
     2401 ccataaagtt ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa
     2461 gctcttcacc gtctgaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt
     2521 tatctacctg atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg
     2581 atactgcaga aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg
     2641 ggtcattcat atccgccagg ctggcgtctg cacttttcag caacgctgca gaggtcgtga
     2701 cgtcctgatc gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca
     2761 gcagccaggt tttagcatcg ctgccggaaa tggtggcgat cttttgttgg acttcatcca
     2821 actgttttgc cagcgtttct tgctgacgat tcgcctgctt aagctgcgca gcttgttgct
     2881 taataatgcc ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt
     2941 ggttagccag ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat
     3001 acaaaccgac acccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcag
     3061 tattgttctt actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc
     3121 tggtctcttc aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg
     3181 agagttattg taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt
     3241 cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac
     3301 agtgtagtaa ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca
     3361 tttcaccgct ggtaacaacg accatcgtca cctcgcggga ttgccagcgc atcgcttctt
     3421 ctgcaccatc gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac
     3481 cgcgcaccgt cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg
     3541 cacgtttgcc cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc
     3601 gatcctgtgg gtagagaatc ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc
     3661 gtccaatggc gaaataagta ggtagttggg gccatttacg atcttgctga tgcagctgtg
     3721 attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg
     3781 ctgccagttg atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa
     3841 aatgccaggc cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag
     3901 acgggcgggt gaccaggata ctcatgccgg ggcctctccg ttatagactt cagcgaggat
     3961 ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatc
     4021 ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag
     4081 cgcacatagc cagatttcgc catcaataag ctcggcgtag ctaccaattg gcacctgaca
     4141 tccgccttcg agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg
     4201 gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca tcaaggcggc attcaatacc
     4261 gactgcacct tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg
     4321 ggactccaga cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc
     4381 gttatctagt ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc
     4441 cgggcggcgt tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc
     4501 tgctggtaac gcatccagac tgtcatagtt attggacaca aaggcatcgc gaggatcttc
     4561 acgctcgcaa atagtgacca gccccagacc ttgcgggaat tcaaccggca catctttcat
     4621 tgaatgtacg gcgatatcag cgcgattctc caggagcgcg acttccagct cttttacaaa
     4681 taagcctttt ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac
     4741 catcggtacc agttcaacga ccaggcccgg atggctcgcc atcaacttgt ctttgacata
     4801 gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa
     4861 catgcttgtt accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt
     4921 cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat
     4981 cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac
     5041 ttgtatgtag cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt
     5101 agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct
     5161 ttgacatacg aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc
     5221 atgacgggta gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga
     5281 gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat
     5341 tccaacaggt ctacagtcta ctgccgacat tgttgcacta tcaccatccg cgaatgccgg
     5401 gttaccttga tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac
     5461 gccactacct gaacgagctt gaactgtatc gtggaatgtc agtacaggat ccaccgaaag
     5521 gtgagcttcc aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt
     5581 gttcctctga cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc
     5641 agttactaca acgtaaatgc agcctgctgg aaagctgggc cgcctcgctg ggtgtggaag
     5701 tcagcttctt cctgattgat gaaaaccgct tccgtcataa tgaaagcggc agcctggggg
     5761 gcgaagattg tggctccacc cagcatatac tgctgcttga cgaattttat cgtactgccg
     5821 tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt
     5881 acgacgacta tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg
     5941 atctcggtgg cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc
     6001 tctacaagag tatcgattcc ccatacaaag cggtgctgaa aacactgctg ctggaagcct
     6061 attcctggga atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg
     6121 acggcgagat tgtatcgttt ggcctcgatc catactgcat gatgctggag cgtgttactg
     6181 aatacctgac ggcgattgaa gatttcaccc gtctggattt agtacgtcgc tgcttctatt
     6241 taaaagtgtg cgaaaaactc agccgtgaac gcgcctgtgt aggctggcgt cgcgcagtgt
     6301 tgagccagtt agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc
     6361 gtgctaactg gaagattgat caggtgcgtg aggcgcacaa cgagctgctc gacgcgatga
     6421 tgcagagcta ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca
     6481 gtccgcagga tatcggcgta ctgacgcgta aactgtatgc cgcgtttgaa gcattgccag
     6541 gtaaagtgac gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct
     6601 ttatttatgt gccgccgggc cgggctaacc gttcaggttg gtatctgtat aaccgcgcgc
     6661 caaatattga gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaataaac
     6721 tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg cacccgtttg tatattaaag
     6781 gtaacggcat tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt
     6841 tcccgctgcg cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagattcgcc
     6901 atctggcgat tatcgttaac ctggaatatg acccgacagc tgcgttccgc aatcaggtgg
     6961 tgcattttga tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg
     7021 taggtagcgt tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca
     7081 acggcgagca atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg
     7141 ccgcaccgcc agatagcgtg gaagtcttct gttatagcca gcatctgcgc ggcttaatcc
     7201 gtactcgcgt gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc
     7261 aggaaaccgg gcgtttcaag gcgctgcgcg tttctggtca aacctggggc ttgttcttcg
     7321 aacgcctgaa tgtatcggta cagaagctgg agaacgccat cgagttttat ggcgctattt
     7381 cgcataacaa actgcacggc ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg
     7441 cggtggtgga cggctttgcc agcgaaggga tcatccagtt ctttttcgaa gaaacgcaag
     7501 acgagaatgg ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtgtatcacc
     7561 actgcgaagg cagcaaagag gagctggtac gtgacgtcag tcgcttctac tcgtcatcgc
     7621 acgaccgctt cacctacggc tcaagcttca tcaacttcaa cctgccgcag ttctatcaga
     7681 ttgtgaaggt tgatggtcgc gaacaggtga ttccgttccg aacaaaatcg attggcaacc
     7741 tgccgcccgc caatcaggat cacgatacgc cgctattaca gcagtatttt tcgtgatgag
     7801 cgtgccggaa agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc
     7861 gcctgttgcg tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa
     7921 atccattcat cgcctttcag gtcaaaatgg tagccgccct gtttggttgc cagccacacc
     7981 tggtgcagcg gctcctggcg gttaataatg attttgctgc cgttttcaaa ggtaatggtc
     8041 agtacgccgc cgttgatttc gcagtcgata tcgctgtcgc catccctgtc gtccagacgc
     8101 tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt
     8161 gtatcctgtt tttaagtgat ggcggcagta tagcggcatg gggtcagggc ttcaaagttt
     8221 gcacctctgc tgctgcgttc cggcatgatt catccgtcac cggaataatg atgtctctgt
     8281 tttgaacttc acgtacgttc tcctctctga cacgagtttt cgccgggcag ggggagggcg
     8341 taatccttac ctctgtatcg gtccatgtat aaacattgcg atcgtcataa gcgaagcgat
     8401 cgtttttagt tagcgaaccg ttaactggaa taatttgcaa gctctttgac cgtaattggc
     8461 ggataaagta agcatgctgt ttgccgctat taaa
//
LOCUS       DAJTRM010000147         8440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRM010000147 DAJTRM010000000
VERSION     DAJTRM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.147"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..187
                     /gene="csgC"
                     /locus_tag="OO322_003754"
     CDS             <1..187
                     /gene="csgC"
                     /locus_tag="OO322_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5908458.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            308..619
                     /locus_tag="OO322_003755"
     CDS             308..619
                     /locus_tag="OO322_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5908459.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            714..1247
                     /gene="ymdB"
                     /locus_tag="OO322_003756"
     CDS             714..1247
                     /gene="ymdB"
                     /locus_tag="OO322_003756"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5908460.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1189..2670
                     /gene="clsC"
                     /locus_tag="OO322_003757"
     CDS             1189..2670
                     /gene="clsC"
                     /locus_tag="OO322_003757"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5908461.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2678..3835)
                     /gene="mdoC"
                     /locus_tag="OO322_003758"
     CDS             complement(2678..3835)
                     /gene="mdoC"
                     /locus_tag="OO322_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5908462.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4229..5764
                     /gene="mdoG"
                     /locus_tag="OO322_003759"
     CDS             4229..5764
                     /gene="mdoG"
                     /locus_tag="OO322_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5908463.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5757..8300
                     /gene="mdoH"
                     /locus_tag="OO322_003760"
     CDS             5757..8300
                     /gene="mdoH"
                     /locus_tag="OO322_003760"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5908464.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2136 a         2001 c         2199 g         2104 t
ORIGIN      
        1 cagttcaggg caaagtcaga cgaagcaaga aaagaccctc tcattgcctg ctaatcaacc
       61 cattgctttg acgaagttga gtttaaatat ttccccggaa gatcgggtga aaatagttgt
      121 tactgtttct gatggacagg cacttcattt atcacaacaa tggacgccct cttcagaaaa
      181 gtcttaattt gttgaaatat cgagcataag atgaatctgg agagaatggt ctgctgcgaa
      241 tcagccaacc tgaaagtatg gataacacaa ccttcaagga tgactaatca ttgaggaaat
      301 agaataaatg ttcagacctt ttttagactc tcttatgctc ggcagtttgt tttttccttt
      361 tattgccatt gcaggaagca ccgcgcaagg gggcgtgatc catttttatg gccaaattgt
      421 ggaaccggca tgtgacgtca gcacccagtc atcacccgta gaaatgaact gcccacagaa
      481 tggttctatt ccgggcaaaa cctactccag caaagcgtta atgagcggca atgtcaaaaa
      541 cgcgcaaata gcctcagtaa aagtgcagta tcttgataaa cagaaaaagc tggctgtaat
      601 gaacatcgaa tataactaag tttctgaaca actcacgttg ctgagcagaa aaatgcgatt
      661 taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa
      721 cgcgtattca tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg
      781 cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg
      841 gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg
      901 ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg
      961 ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga
     1021 atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta
     1081 ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag
     1141 aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa
     1201 acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg
     1261 gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg
     1321 gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg
     1381 ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc
     1441 ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg
     1501 cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt
     1561 tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt
     1621 ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga
     1681 ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta
     1741 gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg
     1801 tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg
     1861 gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc
     1921 aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg
     1981 gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat
     2041 tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt
     2101 atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga
     2161 aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc
     2221 catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa
     2281 ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc
     2341 ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct
     2401 ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc
     2461 gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg
     2521 cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag
     2581 gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg
     2641 tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt
     2701 tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat
     2761 gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa
     2821 gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt
     2881 gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgcgct gactggaagt
     2941 taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg
     3001 tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta
     3061 aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca
     3121 aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa
     3181 atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa
     3241 taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata
     3301 gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc
     3361 gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt
     3421 gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac
     3481 tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga
     3541 tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg
     3601 gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc
     3661 ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca
     3721 catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca
     3781 gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg
     3841 gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc
     3901 catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc
     3961 atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg
     4021 caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt
     4081 tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg
     4141 gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg
     4201 cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt
     4261 aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca
     4321 agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt
     4381 ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg
     4441 gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac
     4501 cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga
     4561 ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc
     4621 cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat
     4681 gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg
     4741 cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt
     4801 ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc
     4861 gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga
     4921 tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac
     4981 cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt
     5041 gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt
     5101 gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg
     5161 tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct
     5221 ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt
     5281 ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca
     5341 gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga
     5401 agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga
     5461 tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt
     5521 taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat
     5581 tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg
     5641 gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc
     5701 gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga
     5761 ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc
     5821 cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc
     5881 gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt
     5941 cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc
     6001 cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt
     6061 tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag
     6121 aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt
     6181 tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt
     6241 atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct
     6301 ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt
     6361 tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg
     6421 gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc
     6481 atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct
     6541 tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca
     6601 ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc
     6661 ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga
     6721 agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca
     6781 tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct
     6841 tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg
     6901 atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag
     6961 ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc
     7021 gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg
     7081 ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg
     7141 gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg
     7201 atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc
     7261 tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc
     7321 tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg
     7381 cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt
     7441 ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga
     7501 agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc
     7561 gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca
     7621 tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt
     7681 caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac
     7741 agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt
     7801 tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca
     7861 gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt
     7921 attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct
     7981 cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg
     8041 caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg
     8101 ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc
     8161 taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat
     8221 attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac
     8281 cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca
     8341 tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca
     8401 catccggcaa aaacaacgag ccaataaaaa taccggcgtt
//
LOCUS       DAJTRM010000148         8348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRM010000148 DAJTRM010000000
VERSION     DAJTRM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.148"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            7..438
                     /locus_tag="OO322_003761"
     CDS             7..438
                     /locus_tag="OO322_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5908465.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO322_003762"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO322_003762"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5908466.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO322_003763"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO322_003763"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5908467.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO322_003764"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO322_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5908468.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OO322_003765"
     CDS             complement(4440..5147)
                     /locus_tag="OO322_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5908469.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO322_003766"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO322_003766"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5908470.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OO322_003767"
     CDS             complement(6005..6208)
                     /locus_tag="OO322_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5908471.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6397..7875
                     /locus_tag="OO322_003768"
                     /pseudo
     CDS             6397..7875
                     /locus_tag="OO322_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8348
                     /locus_tag="OO322_003769"
     CDS             7872..>8348
                     /locus_tag="OO322_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5908472.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTL"
BASE COUNT         1921 a         2305 c         2148 g         1974 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctg
//
LOCUS       DAJTRM010000149         8275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRM010000149 DAJTRM010000000
VERSION     DAJTRM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.149"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..176
                     /locus_tag="OO322_003770"
     CDS             <1..176
                     /locus_tag="OO322_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5908473.1"
                     /translation="EVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEK
                     AYYASIGNDDLAA"
     gene            complement(311..502)
                     /locus_tag="OO322_003771"
                     /pseudo
     CDS             complement(311..502)
                     /locus_tag="OO322_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            500..824
                     /locus_tag="OO322_003772"
                     /pseudo
     CDS             500..824
                     /locus_tag="OO322_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(854..3766)
                     /locus_tag="OO322_003773"
     CDS             complement(854..3766)
                     /locus_tag="OO322_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908474.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3769..3885
                     /locus_tag="OO322_003774"
     CDS             3769..3885
                     /locus_tag="OO322_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5908475.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4249..5091
                     /locus_tag="OO322_003775"
     CDS             4249..5091
                     /locus_tag="OO322_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5908476.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            5094..6182
                     /gene="capU"
                     /locus_tag="OO322_003776"
     CDS             5094..6182
                     /gene="capU"
                     /locus_tag="OO322_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5908477.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            6187..7137
                     /gene="virK"
                     /locus_tag="OO322_003777"
     CDS             6187..7137
                     /gene="virK"
                     /locus_tag="OO322_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5908478.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            7202..8146
                     /gene="lpxM"
                     /locus_tag="OO322_003778"
                     /gene_synonym="msbB"
     CDS             7202..8146
                     /gene="lpxM"
                     /locus_tag="OO322_003778"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5908479.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2234 a         1859 c         1980 g         2202 t
ORIGIN      
        1 cggaggtaat acaccgtaag agctggaaaa accgtgcaga agtggaactg gccacactca
       61 cgtgggtgga ctggtataac aatcgacgat tgctggaaag gctgggccac atccctccgg
      121 cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca gcctgagttc
      181 acagataaaa cactctccag gaaacccggg gcggttcaaa gggccttccc gagagtggta
      241 aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt tgcttaactg
      301 actggcatta aggccgccgg ggacgttttt ttaccaccgg gagtttgggc ataacggtgc
      361 tttcagtaag tacgacaggt actgtggagg cacttccgta aagaagggca tcaagctcct
      421 gctccgacat acctgcaggc aaaggccatg aaagccctga tttccggaag cgagcgaaca
      481 gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg ataagccctc gtcagcagtt
      541 ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca gcgttgcggt gcaccacgcc
      601 tgacggatga actgcgtgct cagggttacc actttaacgt aaaaaccgtg gcggcaagcc
      661 tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt cagcccggtc agttaccgcg
      721 cacatggcct gcccgtgtca gaaaatctgc tggagcagga tttttacgcc agtggtccga
      781 accagaagtg ggcgggagac atcacgtact tacgtacaga tgaagttcgg ttacatcctg
      841 tcagcacgga acctcacgct ttttgagcgg cacgacggat cccccccacc cagtatccga
      901 cagacttttc actgcgttcg taaaaaccct tcttccctcc cagcatatcc actaattccg
      961 gtgacatatc ctgcagataa gtctgcaaat catgccccag atcttccggt gactgcaact
     1021 cataaaccgc gcgccacaca tcacccttcc ttattttaag ccccgggtta agaatatact
     1081 gtccgtggtt cagcctcaga aaaagctttt ttccataagg atttttgctg ttaacttcat
     1141 gttgagagag ctggctgctg atataactcc ttcgtttgcg gcgttccggc agcaacgctt
     1201 caggaagatg aaccacacac tctgccagtc gtgccgcact gaaggcttcc gaaaaaaaca
     1261 gagcacttcc cagatgcgtg tggaacagcg cctgcatcag gatcaccaga aacattgcca
     1321 tctgccggtt gtccagtttg atgaggcgtc cttccacctg cagtgacaga ctttcaggtt
     1381 tcagcagggt atacaactgc gcacttttct gttgcgcata acggggcgta ctgaccgccg
     1441 ctgacagaac ttgatgataa gccgccagcc cgtcattacc ggtcagcaag ggatctgcac
     1501 cacgctccag cagtaactga accagtgcaa tattacctgc ccgggctgct gacatcagcg
     1561 gagtaagatt aaacggcgaa cggtgatcaa caccataacg ttcaatatcc cgcaacacgg
     1621 caaccgggtt attcattgag tatggccgga aatacttcag ctccagtaac tgcagagtct
     1681 tcgttctggg ctgccttgtg ggcttaaaat cttcccggta cagtgcatta tacagcggga
     1741 acagggaata aatcagactg tactccagcg cctgcagttg tatggtttta tcggctgtac
     1801 ctgcgaggat atgctgtttc cactgtcgtg catcctctgc ggtgataatc tgccatggca
     1861 tctcagatgt ctgcaggatc cggctacgga tggtgtccgc ctgctcctgc ttcccctgtt
     1921 tttcaagcag atgtgcggtt ttctgccact catcacggct ggaaatctct tcctgcagat
     1981 tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca cagaggatgc tccacctgct
     2041 gttcaacaag atacacattg tgtttagcac gggtgagcgc aacatacagc gcgttggtaa
     2101 aaaatttata gatttccgct gaacgatcct gcctgtcccg cggacgggaa taacgggcct
     2161 cgccctcaag atcagccggt gtaagaccct cgcaaatatc atcaaatgcc tgccgggcag
     2221 cgcttactat attgtaaagg atcaccgttt cgtattccag ccccttaacc tcctgaaccg
     2281 aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc tttctgttcc ggatgcatca
     2341 caatgaccgc tacacgattc gaaagtgagg ttttggcatt cagttcctgc tttgtctctt
     2401 ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc gcagcttctg acaaaatggt
     2461 gactttcccg atcaatagca ctaaagcgga cctgtttgag tctcagcaac cggttagcca
     2521 gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag gatattaacg gttgtgtcgt
     2581 ttccctgttg ctgccggaaa aagagacttt tgagcgaact ccatgagaaa aagctcggat
     2641 gaactatctg attggcatca ccacagagta aaaactgacc gggatgacgt agcgttttca
     2701 gtaccagttg cagttgaaca ggcgtcatat cctgaacttc atcaacaaaa atgacatcat
     2761 aacggggagt tgccaacgaa agatactgat gactgagcag attactgtca tactgatgcg
     2821 actgtttcag ccatgcgata taacgttcaa acaggacata gaccgtcggg cggtcctcca
     2881 tgccatagag cgactgccgt atccccagtg acagatacgc ctcccgggac agcggcccat
     2941 tcccggaggc aaccgcaccg atcacgcccc tgaactcctc atacagggta tgggcagccc
     3001 ccagtgcggc gatagccctg ttgcggggca gccagtcgct gaacgcactg aaagtcacct
     3061 ccctgccttc cggaatgcgc agagtttcca gaaattccgt cagcgaaaga aagtcaatat
     3121 tctgtacctc actcccttcc cctgaagcgt catagagagt acgcgctttt tccaccagaa
     3181 atgaggaaag gctgagatac agtatatcac cagcagcctg cttcattttt tccagtacca
     3241 gcgccgtctt accacttccc gccgagccaa caatcaccag cggtagcggg tgttcgtaca
     3301 gtgcctgctg atcatcatca aaggacagca ttttatccag gcggtggaaa cgcccatgag
     3361 acggattgat atacgtcagc gcttcagtcg cgatatcaac aggatccggc actggttgtt
     3421 gttgtaagcg cccttcatca atcaccacgt ttctgcgaag aaaacgtgaa gtgttatagg
     3481 catgattgcg aatatattcc agaacaaggc agatagtttc accgtggtag cgatacagcg
     3541 aaaacagtaa tcggtcccgg atactgagcc gggcgcggta aagattggtg tcaattttac
     3601 gaacatcagc ctgaacaaag ttatcggtgg caatagcctg tgcaaatttt ttataacccg
     3661 ggatcgtgtc aggattcaga gtatgataaa gacagatttt catggctgcc ggtttccgtc
     3721 gtgcaaaaaa catgctgatt ttacgaagga atgccttact gaacaacctt gcccggaata
     3781 ccccccgaaa tcagatggaa caatcagaaa aatgctctgc agttatggaa taaagaaggt
     3841 ggggtggtac gccgggaggg cgatccgctg gaaatggcgc agtagctgca ggcctgtcat
     3901 gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg tcaccccgta aataaactct
     3961 gcgctgaaat tccttttttt cattggagta cctgtgttgt tctgaggtga acatatcacc
     4021 tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt cttagtgagt cgtttttaaa
     4081 agatatgata atatatcatt gtttatgaat tgttgatgtt taatttagta aattatatca
     4141 aatatgccat gggtttgcta tgttattcat ccagtatctg gaaataaaat gcaccagctt
     4201 tattgggttt tccttttgtc tctgcttgct aggtgccaga aactttaaat gttaaacgaa
     4261 gggggcattt tgttcaaagc caatcatgtt ccggtattaa tgtatcatca tgtcagtcat
     4321 tgccccggac tggttacact ttctcccgtg acattccgga agcaaataaa atggttggct
     4381 gaaaacaact ggaaaacact gtcatcagat gaactggagt ttttttatcg gggcggaaaa
     4441 ctcccccgga aaagcgttat gttgacattt gatgatggat accttgacaa ctggtttcag
     4501 gtatatcctt tattaaaaga gtttaatctc aaagcacata tttttttaat taccggtttt
     4561 attgggaatg gtccggttcg tcactctcct ggaaaggaat attctcatcg cgattgcgaa
     4621 catcaaatag cgacagggaa tgctgataat gttatgcttc gctggtctga agtaaacgaa
     4681 atgttgcaat ctggtctggt cgaatttcat gttcatacgc atactcatac acgctgggat
     4741 aaaaagttca gttcacgaga agaacagtgt aaacatcttc ggcaggactt gttgagcggg
     4801 agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac atttgtgttg gccggaaggt
     4861 tattataaca aggattatat tcaggtcgct gaagaacttg gattctatta tttatataca
     4921 acagaaagaa gaatgaatgc tcctgctaaa ggaactacac ggataggaag aataagcacc
     4981 aaagaacggg aaagttgcgc atggctgaaa agacgacttt tttattatac aaccccgttt
     5041 ttttcatcgt tgctggcctt tcataagggg ccaagattac cggatgatta ataatgaata
     5101 tactatttac ggaatcatca ccgaatatag gtggtcagga attacaggct gttgctcaaa
     5161 tgaaggccct gaagaaaatg gggcattcag ttctgcttgt ctgcagggaa aacagcaaaa
     5221 ttgcttttga agccagtaaa ttggggattg atatcacatt cgcgttattt cgaaacagtc
     5281 ttcacatccc tactgcatgg agattactcg gaatagttca tggttttcag cccaatgcaa
     5341 tcgtttgtca cagtgggcat gatagcaata ttgttggttt agtacggtta ttcactagga
     5401 aacatccatt cagaattatc aggcaaaaga catatttgac acgaaaaaca aaagtttttt
     5461 caataaatca tttttgcgat gaggtgattg ttcccggaac aagtatgaag acacatctgg
     5521 agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc aggctttgat ttccagaaat
     5581 tatacgttga ttcccgaaac agtttgcctc caaatgttct ttcttggctg gcgtcccgaa
     5641 ggggatgccc cgttattgct caggtaggaa tgttgcgccc ggaaaaaggg cacgaattta
     5701 tgttgaattt actgttccat ttaaaaatga atggacgaca gttctgttgg ttgattgtgg
     5761 ggtctggttc gcctgaactg cgggagcatt tacagtatca gattgacagt atgggcatgc
     5821 atgatgatgt ttttattgct gacaatgttt ttcctgccgc ccccgtatat cgggttgcca
     5881 gtctggtggt tctgccttca gaaaacgaat cgtttggtat ggtactggca gaagcatcgg
     5941 cattttctgt gcctgtactg gccagtcaga ttggtggaat ccctgatgtt attcagaata
     6001 accagaccgg gacattgtta ccagcaggta ataagcacgc atggatgtgc gccctgaatg
     6061 atttttttaa tgaccctggg cgtttttatc agatggctcg ccaggcaaaa caggatatag
     6121 aagagcggtt tgatattaat aaaactgcgt taaaaatact cacattagcg aagcacaagt
     6181 aacaatatgt tttctgtaag taacttatca tttatcggtt tccttaaaag gattgttttt
     6241 tcctcagatt cactcccggg gaagtgggaa cacagaaaat tccggttcat gtacattttg
     6301 cgatgtgcta taaatccggt tgccagtatt cgatattatt acgaactgcg ttccttgcag
     6361 tgcattgagg atattctggc aatacaaccc acgttgccag caagaattca tcgaccttat
     6421 ttgcataaag gggggcgagc ctggtcccgg gggcaatata ttcttgagca ttatcgcttt
     6481 gtgcagaatt tgccagaaaa atattctgaa tttttgtttc cacaaaaatc ggtatcatta
     6541 gtccaattca tcgggaaaga tggagaggat tttgatatcc agtgttctcc cagtggtttt
     6601 gaccgggaag gagagttgat gttgtcattg tttttcaata aaatagtgat tgcccggtta
     6661 acattttcag tcattctgac tcaaaatgga cacaccgctt ttattggcgg attacaggga
     6721 gcccccaaaa ataccggacc tgatattatt cggtgcgcaa cccgggcctg ttatgggctt
     6781 ttcccaaaac ggattatttt tgaagcattt tgtgcgttga tgaaagcttg taatgtctct
     6841 gagtgtctgg cagtcagtga gcacagtcat gttttccggc aattgagata ctggtatcag
     6901 aaacgcaaaa cctttgttgc tgtctacagt gatttctggg agtctgtagc agggaaaacc
     6961 tgtggtgact ggtacaaatt accaacacag gtggttcgaa agccgctaag caatatagct
     7021 agtaaaaaac gctctgagta tcgaaaacga tacgctttgc tggattatat ccatgaaact
     7081 gctatccgct ctttggatgc atatcctgta aactcagaac atcaggactt aaattaaggt
     7141 ttgatgtatt ctctgaataa aatattaatg atgattttgg taggggcatt cgcactaaat
     7201 aatgaaaaaa tacaaatccg agtttattcc tgaatttaag aaaaattatc tttcccctgt
     7261 ttactggttt acatggttcg ttttgggaat gattgcaggt atttcaatgt ttcccccttc
     7321 attcagagat cctgtcttgg ccaaaatagg gcgttgggtg ggcagattga gcagaaaagc
     7381 tcgtcgcagg gcgacgatta atttatcgct ttgtttcccg gaaaagagtg atacagaacg
     7441 ggaaataatt gtcgacaata tgtttgccac agcattgcaa tccatagtga tgatggcaga
     7501 actggcaatt cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat
     7561 tcttgaggaa attcggcaca ataacagaaa tgtgattttt ctggttcccc atggctggag
     7621 cgtggatatt cctgcaatgt tgctggcagc tcagggggaa aaaatggccg ccatgtttca
     7681 tcagcaacga aatccagtga ttgattatgt ctggaattct gtacggcgta aattcggggg
     7741 gcgcttacat tcccgggagg atgggataaa accatttatt cagtcagtac gccagggata
     7801 ctgggggtat taccttccag atcaggatca tggtcctgaa tacagtgaat ttgctgattt
     7861 ttttgcgacc tataaagcga cattaccaat tattggacgt ctgatgaaca tcagtcaggc
     7921 tatgattata ccgcttttcc cggtttatga tgaaaaaaaa catttcctga ctattgaagt
     7981 tcggccacca atggatgcat gcattgccag cgcggacaat aaaatgattg cccgacaaat
     8041 gaacaaaaca gtggaaattt tggtggggtc acatccggaa cagtatatct gggttttaaa
     8101 attgttaaaa acgcgcaaat caaacgaagc ggacccgtac ccttgaaatt ataaagtaca
     8161 ggtatttcca ctagttgttt cttacaggtt accaatcgaa acacatcccc cttccgtgtg
     8221 ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt taaatttaat ttatg
//
LOCUS       DAJTRM010000150         8075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRM010000150 DAJTRM010000000
VERSION     DAJTRM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.150"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(106..1815)
                     /locus_tag="OO322_003779"
     CDS             complement(106..1815)
                     /locus_tag="OO322_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5908480.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO322_003780"
     CDS             complement(2247..2966)
                     /locus_tag="OO322_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5908481.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3647)
                     /locus_tag="OO322_003781"
                     /pseudo
     CDS             complement(3091..3647)
                     /locus_tag="OO322_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3631..3889
                     /locus_tag="OO322_003782"
                     /pseudo
     CDS             3631..3889
                     /locus_tag="OO322_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3998..4576)
                     /locus_tag="OO322_003783"
     CDS             complement(3998..4576)
                     /locus_tag="OO322_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908482.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4997..5234)
                     /locus_tag="OO322_003784"
                     /pseudo
     CDS             complement(4997..5234)
                     /locus_tag="OO322_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5421..5850)
                     /locus_tag="OO322_003785"
                     /pseudo
     CDS             complement(5421..5850)
                     /locus_tag="OO322_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5898..6263)
                     /locus_tag="OO322_003786"
     CDS             complement(5898..6263)
                     /locus_tag="OO322_003786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908483.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
     gene            complement(7016..7489)
                     /locus_tag="OO322_003787"
                     /pseudo
     CDS             complement(7016..7489)
                     /locus_tag="OO322_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(7417..7783)
                     /locus_tag="OO322_003788"
                     /pseudo
     CDS             complement(7417..7783)
                     /locus_tag="OO322_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7785..8075)
                     /locus_tag="OO322_003789"
                     /pseudo
     CDS             complement(7785..8075)
                     /locus_tag="OO322_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2137 a         1939 c         1549 g         2450 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagcaca ggctcgtcag cttcatctgc
     3421 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
     3481 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
     3541 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
     3601 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
     3661 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
     3721 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
     3781 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
     3841 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
     3901 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
     3961 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
     4021 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
     4081 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
     4141 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
     4201 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
     4261 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
     4321 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
     4381 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     4441 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     4501 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     4561 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     4621 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     4681 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     4741 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     4801 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     4861 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     4921 ctccttcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     4981 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     5041 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     5101 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     5161 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     5221 tatcgacagt tcatcgatag gaatttaaat ccccaaaaga ttaaaaaagc accgaagaat
     5281 gcgcgtttat tcaacacaac ctgatcatca agtaatcggt atagacactt aatattgctt
     5341 acattgattg attcaatgta tctctgatgg ttttttgctc cgttcccctc aacatggacg
     5401 caacttcccc tctgcgactc tcagccgcac caccgtattc gagccagcgg ctcatgcatc
     5461 ataacctgct ctgccagacg gtaacccctc ttcagccccg taaaccgcat ctgacctccg
     5521 cgcagctcgc acttcagcgg gtcaaccttc agacactgct gatacatccc tctccttgtg
     5581 atttgcctcg ccgtttttct cactgtctcc gttatggcgt acaccacatc ttccagtaat
     5641 cgccgcttca ccggactcag gaccccgtat caccatccgg aaccccttat ccgccacatg
     5701 ccaggagaac ctttccataa actcgtcgcc actcatcaca aggttttctt ctcgttttgt
     5761 ccggtggctg ttgtaacgca ggccaatttc atcctgaccg gcataatgtt ccaggcggct
     5821 catctgcacc ggctgcttct ccagccagtt aagccatcgg tttccctgct gaaaaatatc
     5881 ggcaaaacgg ggaagcatca gaaaggcgga ataacgccat ccggccagtg aaccgtaccg
     5941 cattcagggc aatacatgcc gtcggcgttg ataccggcag gaattgtcgc aaattcccgc
     6001 tccgtgcagc gggcaatttc cggataccct tcgtcatcaa cacgtacaaa ccagaagacc
     6061 agttttttgt ttccggcatc cacaaagaac ggaatattca ggtctgcgca acactcaacg
     6121 gcatcgtcaa aattatcaaa gcgcagaacc tcagcttctt cttcgtcaaa aaaatcatct
     6181 tcgtgaagtt ttacgacata gcggaggagt ttgcttcttt gaggtgcagg ttttcgtttg
     6241 cgtggtttag ctgaacgggc catataacta cctgaaagac aatgatattg tctgttttta
     6301 taacggtaat cacaggccat gacaagccgc agccgtcagg ccgcctactc ggggcggttt
     6361 agaacgctta tttagtacca gatcttactt ataataaagg ggcttggcag tttggataaa
     6421 ctattgtcct tattccacta atacaaactt tttgtaaagt atattaaata tacttcattt
     6481 catactgaaa tccccggaag tattcaatca aacaattaca ccataaaagg aagagggggc
     6541 cccaattaag ttgtcaagca tgtttactgc gcaatcgctt ggtgctccgt ccctgtaccc
     6601 cgcattggta ctctggcgct catggggctt ccacttgcgc ttctcccttc acatcggggt
     6661 gacaggtcca cgttccccac aagagcctga atcgacttca cgccgctttt atgccggatg
     6721 ccgcgcaggc agtaaacata tcccacagga acgactccac cattgttttg acatcatcta
     6781 tatgctttcg acacatcatc agcggttcac tttcgttcgt ctcttcgaac catacctgat
     6841 cggctcacgt ccgacctttt cccatctcgc tcaccaccac atctcttaaa tgcagcagca
     6901 gtggatggtt tggtgcctgc gcctgcacgc cgacaccaag gggccttccc tcatctcttg
     6961 tgctgcatgg ctgcacaggg actcacgtcc ccaggacgcg ccttcgtggc acacagtcat
     7021 cggcatagca acagtaagca acggcaggtt tccattgcca gttttctttg acagcagtac
     7081 ttcggcctcg ctggatactg tgattccagt accagcgatc ctttctggct tttccactca
     7141 ggtagcgttt attcagatac tggtcgaact cattcagtat gatgttcgac agcaacggcg
     7201 agatcacacc gccctgtggc atactttcac tgaccgcccg gaagagacca acatcaacac
     7261 ggcgttttcc acagcagtct cataaaacgt gtatcactga tcctgcggca gacggctttc
     7321 atcagcagcc ggtgatgtac tgtgtcgaag tagctggaca ggtcaccttc aataacccgg
     7381 cgccccgtgt ttccccgcag tcggtgagct gtaatagtac gtgatgtctc ccgcccactt
     7441 ctggttcggg ccactggcgt aaaaatcctg cgtcagcaga ttttctgata caggcaggcc
     7501 gtgtgcacgg taactgatcg ggctgaactt ctgggaggct tttgcgctca gttcctgacg
     7561 gcgcagactg gctgccacgg tttttacgtt aaagtggtaa ccagagcaca cagttcatcc
     7621 gtcaggcgtg gggcaccgta acgctggact gggtaaaagc cttgcggaca acgctgtcgc
     7681 agtgctgacg gaactgcgga cgtgtgttta tccttgtccg ccgctgacac cacgtatacc
     7741 agccgctgcg ggccacccgg agtacgcggc acattgcctt gatgacggtt tacgcggtcg
     7801 cctgcggcaa cggctttccg gctgacggta gtgtggacct ttttaccccg gagaacaccg
     7861 gcaagcccca taaccgccat gagacgtgcc acagtgcatc tggccactct gataccttcc
     7921 cgtaacaact gatgccagac tttacgcaca gcgtatacct gatgattctc atcgtatacg
     7981 cgcagtatct ctttcttcag ccagtcatcg cgctgcgcac gggcactgcg tttatcatga
     8041 tgatgtcgct gttgctgaca gtggtaatac gttga
//
LOCUS       DAJTRM010000151         7868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRM010000151 DAJTRM010000000
VERSION     DAJTRM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.151"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..1161
                     /gene="rbsA"
                     /locus_tag="OO322_003790"
                     /pseudo
     CDS             1..1161
                     /gene="rbsA"
                     /locus_tag="OO322_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1166..2131
                     /gene="rbsC"
                     /locus_tag="OO322_003791"
     CDS             1166..2131
                     /gene="rbsC"
                     /locus_tag="OO322_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5908484.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2156..3046
                     /gene="rbsB"
                     /locus_tag="OO322_003792"
                     /pseudo
     CDS             2156..3046
                     /gene="rbsB"
                     /locus_tag="OO322_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3172..4101
                     /gene="rbsK"
                     /locus_tag="OO322_003793"
     CDS             3172..4101
                     /gene="rbsK"
                     /locus_tag="OO322_003793"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5908485.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4105..5097
                     /gene="rbsR"
                     /locus_tag="OO322_003794"
     CDS             4105..5097
                     /gene="rbsR"
                     /locus_tag="OO322_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5908486.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5063..6490)
                     /gene="mdtD"
                     /locus_tag="OO322_003795"
     CDS             complement(5063..6490)
                     /gene="mdtD"
                     /locus_tag="OO322_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5908487.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6513..7205)
                     /locus_tag="OO322_003796"
     CDS             complement(6513..7205)
                     /locus_tag="OO322_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5908488.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            7691..>7868
                     /locus_tag="OO322_003797"
     rRNA            7691..>7868
                     /locus_tag="OO322_003797"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2017 a         1893 c         2071 g         1887 t
ORIGIN      
        1 gaaaaccatg tatgtcgaag cggataaatt gctggctaaa cttaacctgc gctttaaaag
       61 cgacaagtta gtgggcgatc tttccatcgg tgaccagcaa atggttgaaa tcgccaaagt
      121 gctgagcttt gagtcgaaag tcatcattat ggatgaaccg accgatgcgc tgaccgatac
      181 cgaaaccgaa tccctgttcc gcgtcatccg cgagctgaaa tcgcaaggcc actgtattgt
      241 ctatatctcc caccgcatga aagaaatctt cgagatttgc gatgacgtta ccgtttttcg
      301 tgatgggcaa tttattgctg agcgcgaagt ggcatcactg accgaagatt cgctgattga
      361 gatgatggtg ggccgcaagc tggaagatca atatccacac ctggacaaag cgccaggaga
      421 tatccgcctg aaagtcgata atctctgcgg acctggcgtt aacgatgtct cttttacttt
      481 acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc gcaggtcgta ccgaactgat
      541 gaaagtgctc tacggcgcac tgccgcgcac cagcggttac gtcaccctgg atgggcatga
      601 agtcgttacc cgttcaccgc aggatggcct ggcaaacggc attgtgtata tctccgaaga
      661 ccgtaaacgt gacggtttag tgctgggcat gtcagtaaaa gagaacatgt cgctgacagc
      721 gctgcgctac ttcagccgcg ctggcggcag tttgaagcat gccgatgaac agcaggctgt
      781 gagtgatttc attcgtctgt ttaatgtgaa aacgccgtcg atggaacagg caattggtct
      841 gctttccggt ggcaatcagc aaaaagtggc gattgcccgc ggtctgatga cacccccaaa
      901 gtattgatcc ttgatgagcc tacccgtggc gtagatgtcg gcgcgaaaaa agagatctat
      961 caactgatta accagttcaa agccgatggc ttgagtatca ttctggtgtc atcggagatg
     1021 ccagaagtat taggcatgag cgatcgcatc atcgtcatgc atgaagggca tctcagcggg
     1081 gaatttactc gtgagcaggc cacccaggaa gtgttaatgg ctgtcgctgt gggcaagctt
     1141 aatcgcgtga atcaggagta aaaaaatgat aacccagact gtctctggtc gccgttattt
     1201 cacgaaagcg tggctgatgg agcagaaatc gcttatcgct ctgctggtgc tgatcgcgat
     1261 tgtctcgacg ttaagcccga actttttcac catcaataac ttattcaata ttctccagca
     1321 aacttcggtg aacgccatta tggcggtcgg gatgacgctg gtgatcctga cgtcgggcat
     1381 agacttgtcg gtaggttctc tgttggcgct aaccggcgca gttgctgcat ctatcgtcgg
     1441 cattgaagtc aatgcgctgg tggctgtcgc tgctgctctc acgttaggtg ccgcaattgg
     1501 tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag gcgtttatcg ctacgctggt
     1561 tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc aacggtagcc cagtgaatac
     1621 cggctttact gagaacgccg atctgtttgg ctggtttggt attggtcgtc cgctgggcgt
     1681 accgacgcca gtctggatca tggggattgt cttcctcgcg gcctggtaca tgctgcatca
     1741 cacgcgtctg gggcgttaca tctacgcgct gggcggcaac gaagcggcaa cgcgtctttc
     1801 tggtatcaac gtcaataaaa tcaaaatcat cgtctattct ctttgtggtc tgctggcatc
     1861 gctggccggg atcattgaag tggcgcgtct ctcctctgca caacccacgg cggggactgg
     1921 ctatgagctg gatgctattg ctgcggtggt tctgggcggt acgagtctgg cgggcggaaa
     1981 aggtcgcatt gttgggacgt tgatcggcgc attaattctt ggcttcctta ataatggatt
     2041 gaatttgtta ggtgtttcct cctattacca gatgatcgtc aaagcggtgg tgattttgct
     2101 ggcggtgctg gtagacaaca aaaagcagta ataacgacta caggacatct tgaatatgaa
     2161 catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta agcgccaccg tcagtgcgaa
     2221 tgcgatggca aaagacacca tcgcgctggt ggtctccacg cttaacaacc cgttctttgt
     2281 atcgctgaaa gatggcgcgc agaaagaggc ggataaactt ggctataacc tggtggtgct
     2341 ggactcccag aacaacccgg cgaaagagct ggcgaacgtg caggacttaa ccgttcgcgg
     2401 caccaaaatt ctgctgatta acccgaccga ctccgacgca gtgggtaatg ctgtgaagat
     2461 ggctaactag gcgaacattc cggttatcac tcttgaccgc caggcaacga aaggtgaagt
     2521 ggtgagccac attgcatctg ataacgtact gggcggcaaa atcgccggtg attacatcgc
     2581 gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa ggcattgccg gtacatccgc
     2641 agcccgtgaa cgtggcgaag gcttccagct ggccgttgct gctcacaagt ttaatgttct
     2701 tgccagccag ccagcagatt ttgatcgcac caaaggtttg aacgtaatgc agaacctgtt
     2761 gaccgctcat ccggatgttc aggctgtatt cgcgcagaat gatgaaatgg cgctgggcgc
     2821 gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg gtcgtcggat ttgacggtac
     2881 accggatggc gaaaaagcgg tgaatgatgg caaactagca gcgactatcg ctcagctacc
     2941 cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa gtgctgaaag gcgagaaagt
     3001 tcaggctaag tatccggttg atctgaaact ggttgttaag cagtagtttt aatcaggttg
     3061 tatgacctga tggtgacata aatacgtcat cgacagatga acgtgtaata taaagaaaag
     3121 cagggcacgc gccaccctaa cacggtggcg cattttatgg acatcccgaa tatgcaaaac
     3181 gcaggcagcc tcgttgttct tggcagcatt aatgctgacc acattcttaa tcttcaatct
     3241 tttcctactc caggcgaaac cgtaaccggt aaccactatc aggttgcatt tggcggcaaa
     3301 ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga atatcgcgtt tattgcctgt
     3361 actggcgatg acagcattgg cgagagcgtt cgccagcagc tcgccactga taacatcgat
     3421 attactccgg tcagcgtgat caaaggcgaa tcaacaggtg tggcgctgat ttttgttaat
     3481 ggcgaaggtg agaatgtcat cggtattcat gccggcgcta atgctgctct ttccccatcg
     3541 ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag cgttattaat gcagctggaa
     3601 tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc atcaaaataa gactatcgtt
     3661 gcgcttaacc cggctccggc tcgcgaactt cctgacgaac tgctggcgct ggtggacatt
     3721 attacgccaa acgaaacgga agcagaaaag ctcaccggta ttcgtgttga aaatgatgaa
     3781 gatgcagcga aggcggcgca ggtactgcat gaaaagggta tccgtactgt actgattact
     3841 ttaggaagtc gtggtgtatg ggctagcgtg aatggtgaag gtcagcgcgt tcctggattc
     3901 cgggtgcagg ctgtcgatac cattgctgcc ggagatacct ttaacggcgc gttaatcacg
     3961 gcgttgctgg aagaaaaacc attgccagag gcgattcggt ttgcccatgc tgccgctgcg
     4021 attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt ggcgtgaaga gatcgacgca
     4081 tttttagaca ggcagaggtg acgcttggct acaatgaaag atgttgcccg tttggcgggc
     4141 gtttctacct caacagtttc tcacgttatc aataaagatc gcttcgtcag tgaagcgatt
     4201 accgccaaag ttgaagcggc gattaaagaa ctcaattacg cgccatcagc tctggcgcgt
     4261 agcctcaaac tcaatcaaac acataccatt ggcatgttga tcactgccag taccaatcct
     4321 ttctattcag aactggtgcg tggcgttgaa cgcagctgct tcgaacgcgg ttatagtctc
     4381 gtcctttgca ataccgaagg cgatgaacag cggatgaatc gcaatctgga aacgctgatg
     4441 caaaaacgcg ttgatggctt actgttactg tgtaccgaaa cgcatcagcc ttcgcgtgaa
     4501 atcatgcaac gttatccgac agtgcctacc gtgatgatgg actgggctcc gttcgatggc
     4561 gacagcgatc ttattcagga taactcgttg ctgggcggag acttagcaac gcaatatctg
     4621 atcgataaag gtcatacccg tatcgcctgt attaccggtc cgctggataa aactccggcg
     4681 cgcctgcggt tggaaggtta tcgggcggcg atgaaacgtg cgggtctcaa cattcctgat
     4741 ggctatgaag tcactggtga ttttgaattt aacggcgggt ttgacgccat gcgccaactg
     4801 ttatcacatc cgctgcgtcc tcaggccgtc tttaccggaa atgacgctat ggctgttggc
     4861 gtttaccagg cgttatatca ggcagagtta caggttccgc aggatatcgc ggtgattggc
     4921 tatgacgata tcgaactggc aagctttatg acgccaccat taaccactat ccaccaaccg
     4981 aaagatgaac tgggggagct ggcgattgat gtactcatcc atcggataac ccagccgacc
     5041 cttcagcaac aacgattaca acttactccg attctgatgg aacgcggttc ggcatagatt
     5101 tacgctgtct tttgatcaaa ttattaccat cggttgtttt cagaagcatg aacattgctg
     5161 ctgaagcaac agtaataatg cccatcgtga taaacgtata gtggaattgt tcgacagtcg
     5221 ttgtgccttc catcccttca taaatgcgaa ggacggccgc acttacagca acgcctaaac
     5281 taatcgacag ttgctgcgtg accgccagaa cactgttacc gctgctggcg ttgtcatcgg
     5341 tcagatcggc aagtgtgatg gtattcatcg cggtaaactg cgtcgacata gccatcccta
     5401 atataaacaa cggcaagatc agcatccata tcgccattgc cggtgattgc aaagagaact
     5461 gagcgatcat tagcccaata atcaccgtga tccccactaa tgtatggcga tagcccagac
     5521 gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct gtcggtgcca
     5581 tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc aatggcataa
     5641 ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata ccgatcgaga
     5701 aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtacgt cgcgcatgga
     5761 gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggca atccagctgg
     5821 cgacaatctt ttccccgaat agctctattc cgcttgaaaa gagaacaagg ctgaggccaa
     5881 acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg ggcatatgtt
     5941 tgcgcgcgta aagaaggccc gcaataccta tggggatatt aattaaaaat atccagtgcc
     6001 aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc cccaccagac
     6061 ccggcatggc gacaaaattc aataccggaa gaagttcatt acgaggataa gcacgcagta
     6121 aggccggccg agcaacaggc atcatcattg cgtcgcctat cccttgaata acccggaaga
     6181 caaccagctg tggtagcgaa ttagaaagtg cgcaggccag agaacccaac gtgaacagac
     6241 tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc ggctagccat ccgcttaccg
     6301 gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc gcgagaggag
     6361 aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg gcatcaagtg
     6421 cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgacatacta cgcttctttt
     6481 tatcgctcat tcaatgtcct gttatcgggt tatcacttat caggtgagcg tagcagcgcc
     6541 tgacaagctt taaatgccgc gtcgccatcg ctttggataa tcgcatcgac aatcgcctgg
     6601 tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt gtgataaacc
     6661 gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc atagatatgc
     6721 tcatgccagg ccatatcgac ttcgatccag cgttcacggc gaaagttctc ttttaatgcc
     6781 gccatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc aaccgttgct
     6841 gccagtaggc aggcttgcgg ctccagacag atacgcataa ccagaaagtg atcgatgacc
     6901 tgatgaaagt tctcttctgt tatccaccaa gtaagcaatt cctgatcaag aaaattccag
     6961 ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg gtaaaaccat cccttttgcc
     7021 gttaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg ctcgcccagc
     7081 tcaatctcac cgggcaaaat ggtgccgggt tcatattcac cttttaagat ccgttgcgcc
     7141 agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg tgcgcttaaa
     7201 ggcattactt atcttccttt ttctttttat tcctccttag tatgccacca ggaagtgtga
     7261 ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg tgttttgctg
     7321 aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg gataactccc
     7381 tataatacgc caccactgac acggaacaac ggcaaatacg ccgccgggtc agcggggttc
     7441 tcctgagaat tccgacagaa aaagcaaaaa taaatgattg actctgtagc gggaaagcgt
     7501 attatgcaca cccccccccg cgccgctgag aaaaagcgaa gcgcactgct ctttaacaat
     7561 ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgaaaa
     7621 atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgagca
     7681 tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta
     7741 acacatgcaa gtcgaacggt aacaggaagc agcttgctgt ttcgctgacg agtggcggac
     7801 gggtgagtaa tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct
     7861 aataccgc
//
LOCUS       DAJTRM010000152         7783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRM010000152 DAJTRM010000000
VERSION     DAJTRM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.152"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..1333
                     /gene="yjeM"
                     /locus_tag="OO322_003798"
                     /pseudo
     CDS             1..1333
                     /gene="yjeM"
                     /locus_tag="OO322_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1484..4795)
                     /gene="mscM"
                     /locus_tag="OO322_003799"
     CDS             complement(1484..4795)
                     /gene="mscM"
                     /locus_tag="OO322_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5908489.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4817..5785)
                     /gene="psd"
                     /locus_tag="OO322_003800"
     CDS             complement(4817..5785)
                     /gene="psd"
                     /locus_tag="OO322_003800"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5908490.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5882..6934)
                     /gene="rsgA"
                     /locus_tag="OO322_003801"
     CDS             complement(5882..6934)
                     /gene="rsgA"
                     /locus_tag="OO322_003801"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5908491.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7029..7574
                     /gene="orn"
                     /locus_tag="OO322_003802"
     CDS             7029..7574
                     /gene="orn"
                     /locus_tag="OO322_003802"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5908492.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1762 a         2019 c         2104 g         1898 t
ORIGIN      
        1 ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggtggt atctattcct
       61 ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg tggttttcct
      121 cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc tcaacattcc
      181 tctatggtag tgacatgacc cagcactggc gtattgccgg actggagcct acgcaggtgg
      241 ttggtctgct ggcagtagca tggatgattc tggtcacagt cgttgcttca aaggggatta
      301 ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg aatttagtat
      361 tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg caggatatta
      421 atttccttgc atcaccgaac ccgggttatc agtccggtct ggcaatgcta tcgtttgtgg
      481 tatttgctat ttttgcctat ggcggaattg aagcggttgg tggtctggtc gataaaacgg
      541 aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt atttcaatcg
      601 gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag gtattaagta
      661 atggttccgt taaccttggc aatattacct atgtgctgat gaagagcctc ggggtgatgc
      721 tgggtaacgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta tggtttgcgc
      781 gtattaccgg actttcgatg ttcctcgctt ataccggtgc gtttgctatt caccgctgaa
      841 agccatcatc caggggacgc cgaaagcgtt gtggccggaa ccgatgacgc gcctgaatgc
      901 gatggggatg ccgtctatcg ccatgtggat gcagtgcggg ttggttactg tcttcattct
      961 gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt aacaagctga cgctgatggc
     1021 gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg gctttcccgt tttttaaagc
     1081 acgtcaggat ctcgacagac catttgtgat tttcaaaacg cgtatgtcgg caatgattgc
     1141 gacggtggtt gtcgttctgg tggtgacatt tgcgaacgtc ttcaccatta ttcagcctgt
     1201 ggttgaagcc ggagactggg acagcacatt gtggatgatt ggcggccctg tcttcttctc
     1261 gctgttagcg atggcgattt accagaacta ttgcagtcgc atggcgaata aacctgagtt
     1321 agctctcgac tgatcatatg aaatcctgca actcatcgtc tctccggcga tgagttgtca
     1381 ggaagtaatg cgtatggatg atactctcca gggccggtga taactgaaga tgatgttaag
     1441 ctgctaaatg agccacgttg acatcatttg gcagcttcat gcattacaaa cttcccgcct
     1501 gacgaccttt gcccgcagac gtcagcgttc tccccgtttg tttaccgttg aggctttcca
     1561 gacgcatctg gaagggcggg aatggcatat cgataccgtg ggcgtggaag cctgccagaa
     1621 tcagctggtg gatctcatgg cgtagcggca tacggtgacc catctcagcg gcgtaaatac
     1681 gcagctcgaa aatctgaatc ccctgttgca gatccaccag gaagacttcc ggtgccgggt
     1741 tgtcgatcac cagcgagcag cgacgcgctg cggtgagcag gatttccgtc acttcttcac
     1801 tgttggcatc ggcaggggcc ggaatcgtca acactacgcg cgtgaccgag tcagagagcg
     1861 accagttgat aaactgctcg gtaataaacg ccttgttcgg cacgattatc tctttacggt
     1921 cccagtcgct gatggtggtg gcgcgggtgt taatttttgt cacgctacca gtaagatcgc
     1981 ggatcgttac cgtatcgcca atgcgaatcg gtttttcgaa caggatgatc aggccagaga
     2041 taaagttggc gaaaatttcc tgcaaaccaa aaccgagacc aacaccgagc gcggcaacca
     2101 gccactgcaa tttcgaccac tcaataccaa tcattgagaa gccgaccagc ccgccaatca
     2161 gcatcagcag atattttgtg atggtggtga tggcgtaacc cgtacccggc gttaaatcca
     2221 ggtgctgcaa aatcgccagt tccagcagcg cgggcaggtt gcgcaccagc tgcgtggtga
     2281 tgataaatac cagaatggca atcagcaccg caccgagggt aattggctcc agactttcta
     2341 cgccctgtac cgtggaggtg acatcccaca gcgaaatatt ttcgaggaag ccgaaagcgg
     2401 aatggatttc tgaccacagc acaatgaccg aaagcagggc gatcaacatc aaaattgagc
     2461 gcaccagccg caaggattgc gcactgatgg catcgagatc gacttcgctt tcatcgactt
     2521 caattgctcc ttccgggcta ctgtgatgat gcgcttcctc ttcgccacgc gcacgttgcg
     2581 ctaacatctc tgcccggcga tgcttcgccc ggtcaaacgc cagcctgcga cgctggatca
     2641 gcatccagcg gcggataacg tgataaacca ccagtagcag gaaccagatg gcaaccgagg
     2701 tttcaagcct cgctaacagt gcctgcgccg ttgccagata acccaccgcc gacgccagaa
     2761 tggcaaccag tggtgcgcca atcatcatgt tccacagcat atggttggta atgttgtcgc
     2821 cgctgccttc tttgttgaga tacaacggaa taccggcttt tttcaggctt agggtgacca
     2881 ccgccagcgc accgcaaatg aggataaagc aaagccgtcc cagcgaaccg gaaaattcgc
     2941 ggtcgtcgag gttatcgaac atcatcagcg ccataatcag cccgatgctc atcaggtagt
     3001 agcgcatccc acgggaaaca cgttcgcgcg gccagccaaa atgagcgata aacaagccgt
     3061 tcgggcgggc aaaggtggcg caaatcatca ctacccacag cagcggcacg gtggccgtta
     3121 caccatcgcc aatcgcgacc gccagcggat aaggccacgc ctcgcgcaag ccgtaaccca
     3181 gcgtcatcca cagcaccggt aacggtgacg cgacgagaat cgaccagaaa agagtgcgca
     3241 acgtcagcca gaagtgatcc tgagtcactt tgccgacttt cgccgccgaa cgttcaagaa
     3301 aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac cagaatcaac gcgccaaaca
     3361 gcggcaaaat tgtctctttg ctggtcagca tcatcacact ggctttgccc aactgactga
     3421 aggtgtccag cgaaatgaga cgacgcagat cctgggcgat ttccagcggc caggcgatgg
     3481 tcatcgggcg cacgtcagag gcccagaaca gatagcggtg cgttgcttcg ttcacctctt
     3541 tcagcgcatc ctccagttgc ctgttggaga ctttcagctt ggtcagttcc aatagtagcg
     3601 tgtcgccacc ctgcaacaat gagttcagca actcacgctg agtgcgcagt tgtgcttcca
     3661 gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc ctgatggatt tgccgtagca
     3721 gcggctgttt attaagtaaa tcctcataac gtaaccgttg cacacgcaac tgcgccattt
     3781 cggtatcaag ctgttgtggt tttggcattt ctggtagccg tgccacctgc gcccgcagcg
     3841 cttcgccgag caggttggac gatcccagcc attgcgactg ttcacgcagc gtattcaacg
     3901 cctgccggac ctgtaacgtc tggctggcag cctgacgctg ttgcgaggca acgagctcca
     3961 tccgctgcgc ctgttgattc agcgctgccg atagttcgcg gttaattttg aattgcgcga
     4021 cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa ttcggtactt tccagcgccc
     4081 gctccgcctc aagctgacgt tggctattca actgattacg taaggcctgc aaatacgcat
     4141 ccagttgctg gctctctttt tccgccagct ctgagcgtaa gcgcgctaat tcctggcggt
     4201 tattggcaga cagctgcgcc agctccagtt catcaacgag cgccttaaga cgtgcagagt
     4261 cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt attgccggta agcgttccca
     4321 ggcggcgctc gatctcattt aactgacggc gggcgtcggt ttgctgttgc ggcagttgat
     4381 tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg ctgggcctga cggcttttat
     4441 ccagcaactg gctgctgacc tggagaattt cctgattcag cgcgtcggta gacattcccg
     4501 gcgacacgct gcgcggctcg tcacgaatgt tgtttaattg tgcgcgcaga gtagcggaga
     4561 gtttcggata attatcgata acttgctgat attgtttgat gcgctcaagg gaaccttttc
     4621 gttcctcaag cgcatttaag gcagactgga gcgcctctac gacttccggc tgtgcgggtt
     4681 tcgccgcttt tgcctgctcc agttcctgag tgatttgttt gctatcgggg gccgtcgcgg
     4741 cgtacgcccc ccaactgagg caccaggcca tcagaaaagt gataatcagg cgcacgtcag
     4801 cgtttccttt gatggattag acctggtctt ttttatcgtc aaccaatggg ctggcgtcgt
     4861 gttctgcttc gatctcttca gcaggaagtg gggcgggttc agcgtctggc gtaacaaagg
     4921 tttcggtaga tactgccagc ggctggccaa ttttcgtgac agacaggctt tccagttgct
     4981 caaccagatt cactttaccc ggtgcaaaca ggttgataac ggtggaaccg agtttaaagc
     5041 gacccatttc ctggcctttc agcagtgcca cagaaccgtc gttttccccg gcaggccagg
     5101 tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt gcccgcccag accgtctcaa
     5161 tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat tgggccaaat tcggtatcga
     5221 aaaggcaaat cacgcgttcg ttacgggcaa acagatttgg cacgttctga gcggtgagat
     5281 ggttaacgga aaagagatcg cccggcacgt agatcatctc acgcagaata ccgttgcacg
     5341 gcatgtgtac gcgatggtag tcacgcgggg agaggtaagt ggtcacaaac gtaccgttgc
     5401 ggaacaggtc cgccatcaga tagttgcctg ccagcagggc ttcgaggctg tagttgtggc
     5461 ctttggcttg caggatttta tcttcttcga ttttacccaa ctggctgata acgccatcgg
     5521 caggcatgac cagtacattc ggatcggtat cgattgggcg tacttcgtca cgcagcggac
     5581 ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc cggcttttgc gcctctttca
     5641 tgtcgacctt gtagtattta acgaacagat cgataaccag ttttgtcagc catcctgccc
     5701 gcttgcttgc gccccaaccc gccaggcgag taagccatag tttcggcaga atgtactgta
     5761 gcgaaagttt aaatgaattt aacaaggtag cctccaggcc attgttttgt cgttcctgat
     5821 ccggccaaca tgccggatcc tgaaaaaaag gggacgattc taacgacggt tagcttaatt
     5881 gtcagtcatc cgtatcagaa aagtttttac gcgtttttac ctgcgccatg ctttccagaa
     5941 tacggtgata gttttcgaaa cgggtttccg cgattttccc ttcctcaacc gcttcccgga
     6001 tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata tttacacaga cctaaatagt
     6061 catggaattc gacaaagccc tgagtgattt gttccggctc caggtgccag aggccgaact
     6121 cacgcacacc aggggaatca atcacatcac cgccgtgcgg gaagtgatac agccgagcgg
     6181 cggtcgtggt gtgctgaccg agccccgagt tgtcagagac atcgttggtc aggatctctt
     6241 tttgcagccc cagcaacgcg ttcagcaggc tggatttacc gacgccagac tgcccggcaa
     6301 aaatgctgat gcgcccggtc aacgcctctt ccagcggttt cagcccatcc tgagtatggc
     6361 tggaaaccat caatacgcga taaccgatgt tgcggtagat atccatctgc tcgttgacga
     6421 acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag cacgataatc ggctcaatct
     6481 gcaaggtttc gcaggccacc aggtaacggt cgataatatt gagcgacagc tccggcaaaa
     6541 tggcggagac aatgacaatc tggtcgatgt tggcggctat gggtttcacg ccgtcgtaga
     6601 aatccggacg cgttaacacc gaggtgcgct catgcaccgc ttccacgatc cctttgacat
     6661 ttacgccttc cgccgccggt ttacccggac gccagactac gcggtcgccg gttaccagcg
     6721 aacggatggt acggcgaata ttgcagcggt gaacgtcgcc atcggcggat tccacatcag
     6781 cgtgcatacc aaagcggctg atgacgatac cttcatcagg ctcgccaaac agattgtcgt
     6841 cgtagtcggg cttctcctta gacgttttaa gacgacgctg gtgattggcg ttcacgcggc
     6901 gctgctggcc tttggagagt ttatttttac tcaatcgtac tgactcctgg tcgcccctga
     6961 tgggcaaaac atctatgata cacgcaattg tggatcaata tagtcactgt gaatgggtgg
     7021 aaaatagcat gagtgccaat gaaaacaacc tgatttggat cgatcttgag atgaccggtc
     7081 tggatcccga gcgcgatcgc attattgaga ttgccacgct ggtgaccgat gccaatctga
     7141 atattctggc agaagggccg accattgcag tacatcagtc cgatgaacag ctggcgctga
     7201 tggatgactg gaacgtgcgc acccataccg ccagcgggct ggtagagcgc gtgaaagcga
     7261 gcacgatggg cgatcgggaa gctgaactgg caacgctcga attcttaaaa cagtgggtgc
     7321 ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca ggaccgtcgt ttcctgttta
     7381 aatacatgcc ggagctggaa gtctacttcc actaccgtta tctcgatgtc agcaccctga
     7441 aagagctggc gcgccgctgg aagccggaaa ttctggatgg ttttaccaag caggggacgc
     7501 atcaggcgat ggatgatatc cgtgaatcgg tggcggagct ggcttactac cgcgagcatt
     7561 ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg gaatccactt gcggcggcgt
     7621 gattttgacg ctaaatcgaa cgtcttgctg ataatttcag cacttgagat aaaaacgcaa
     7681 aaaaaacttt tttggggggt tgcagaggga aagatttctc gtataatgcg cctcccgtaa
     7741 cgacgcagaa atgcgaaaat tacgaaagca aaattaagta gta
//
LOCUS       DAJTRM010000153         7751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRM010000153 DAJTRM010000000
VERSION     DAJTRM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.153"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OO322_003803"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OO322_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OO322_003804"
     CDS             1128..1235
                     /locus_tag="OO322_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908493.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OO322_003805"
     CDS             complement(1288..2046)
                     /locus_tag="OO322_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5908494.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OO322_003806"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OO322_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5908495.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OO322_003807"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OO322_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5908496.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OO322_003808"
     CDS             3759..4619
                     /locus_tag="OO322_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5908497.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OO322_003809"
     CDS             complement(4660..5196)
                     /locus_tag="OO322_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5908498.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OO322_003810"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OO322_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5908499.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OO322_003811"
     CDS             6174..6764
                     /locus_tag="OO322_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5908500.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..>7751
                     /gene="tcyP"
                     /locus_tag="OO322_003812"
     CDS             6897..>7751
                     /gene="tcyP"
                     /locus_tag="OO322_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5908501.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     QSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGIN"
BASE COUNT         2060 a         1837 c         1827 g         2027 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa t
//
LOCUS       DAJTRM010000154         7466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRM010000154 DAJTRM010000000
VERSION     DAJTRM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.154"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..735
                     /gene="ydfJ"
                     /locus_tag="OO322_003813"
     CDS             <1..735
                     /gene="ydfJ"
                     /locus_tag="OO322_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5908502.1"
                     /translation="LKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAG
                     NSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIM
                     NTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRF
                     TRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKD
                     RDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            824..2284
                     /locus_tag="OO322_003814"
     CDS             824..2284
                     /locus_tag="OO322_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5908503.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2320..2523)
                     /gene="ydfZ"
                     /locus_tag="OO322_003815"
     CDS             complement(2320..2523)
                     /gene="ydfZ"
                     /locus_tag="OO322_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5908504.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2701..3387)
                     /gene="ydfH"
                     /locus_tag="OO322_003816"
     CDS             complement(2701..3387)
                     /gene="ydfH"
                     /locus_tag="OO322_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5908505.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3476..4222)
                     /gene="ydfG"
                     /locus_tag="OO322_003817"
     CDS             complement(3476..4222)
                     /gene="ydfG"
                     /locus_tag="OO322_003817"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5908506.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4359..6404
                     /gene="dcp"
                     /locus_tag="OO322_003818"
     CDS             4359..6404
                     /gene="dcp"
                     /locus_tag="OO322_003818"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5908507.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6449..6967)
                     /locus_tag="OO322_003819"
     CDS             complement(6449..6967)
                     /locus_tag="OO322_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5908508.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7243..>7466
                     /gene="ydeI"
                     /locus_tag="OO322_003820"
     CDS             7243..>7466
                     /gene="ydeI"
                     /locus_tag="OO322_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5908509.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1886 a         1755 c         1870 g         1955 t
ORIGIN      
        1 ctgaaagaaa gcccggtctt tgagaaggtt aacgacagca accaaccgac agcaaaacct
       61 gcacctgctg gtagcatgtt ccagagcaaa tccttctggc tggcaacagg gctgcgtttt
      121 ggtcaggcgg gtaactccgg tttaattcag actttccttg caggctattt agtacagacg
      181 ttattgttta acaaagcaat tccaacagat gcattgatga tcagttcgat tctcggcttt
      241 atgaccattc cgttccttgg ttggttatcc gataaaattg gtcgccggat cccgtatatt
      301 attatgaata cctctgcgat tgtgctggcg tggccaatgc tttctatcat cgtagataaa
      361 agctatgccc cgagcaccat tatggttgca ctgattgtga ttcataactg tgcggtgctg
      421 ggattatttg ctctggaaaa cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc
      481 acccggatgg ctatttcaaa agaaattggt ggtcttatcg cttccggttt tggtcctatc
      541 ctggcgggta ttttctgcac catgacggaa tcctggtatc cgatcgccat tatgatcatg
      601 gcatattcag tgattggttt aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat
      661 ttaagtgcgc tggaagacgc tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct
      721 tccagaagtc tgtaaaccct caatcccttc tcttaccgga gaggggattt ttattcatat
      781 aacaaaacat atagcttgcc atatttaaat ttaaggaatt gttatgggaa ataatttgtt
      841 atcagcaaaa gcgacactcc ctgtttatga tcgtaataac ctggccccaa gaattgttca
      901 tttaggcttt ggtgcatttc accgtgcgca tcagggcgtg tatgccgata ttcttgctac
      961 agaacatttc agtgactggg gatattatga ggtcaactta atcggcggcg aacagcaaat
     1021 tgccgattta caacagcaag ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg
     1081 gacggctcgc gtcgttggcg tcgttaaaaa agccttgcac gtacagatgg atggcttaga
     1141 aaccgtgttg gctgcgatgt gtgaaccgca aatcgcgatt gtctctctga caatcaccga
     1201 aaaagggtat ttccactctc cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc
     1261 tgccgacgtg caaaatcccc accagccgaa aacagcaaca ggggtgattg ttgaggcgct
     1321 ggctcgccgt aaagcggcag gacttcccgc atttaccgtc atgtcatgtg acaacatgcc
     1381 agaaaacggt catgttatgc gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa
     1441 actggcacaa tggatcgaag ataacgtgac tttcccatca acaatggtgg accgtattgt
     1501 gcccgcagtg acagaggata cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc
     1561 tgctggcgtt gcctgtgaac ctttccgcca gtgggttatc gaagataact ttgtagccgg
     1621 acgtccggaa tgggaaaaag cgggagccga actggttagt gatgtgctgc cttatgaaga
     1681 gatgaagttg cgcatgctca acggcagtca ttcattcctg gcatatctgg gttatcttgc
     1741 tggatatcag cacattaatg actgtatgga agatgaacat tatcgtcatg cggcgtatgg
     1801 catgatgttg caggaacaag cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta
     1861 cgctaaccga ttaattgcac gctatagcaa cccggcgtta cgtcatcgaa cctggcagat
     1921 tgcgatggat ggcagccaga aattgccaca gcggatgttg gattctgttc gctggcatct
     1981 ggcgcatgac agcaagttcg atctgctggc gctgggcgtc gcgggttgga tgcgttatgt
     2041 cggtggtgtt gatgaacagg gaaatccgat agaaatcagt gacccactgt tacctgttat
     2101 tcagaaggct gtacaaagta gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat
     2161 taaggcaatc tttggtgatg atttgccaga caatagcttg tttactgcaa aagtgacgga
     2221 agcgtacttg tctttattag cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa
     2281 gtaaaaaaag caatgccgcc atcggcggca ttcacacact tagttcatgc cgagacgaat
     2341 caggtccaga ggtgccagtt tctcttcaca accttcaaca actacggttt ttccgcggcg
     2401 gatttgctca gcggtcagac cttcagtatc aatcgacagg atcttgccag tgtgaccggt
     2461 gccgctaacc ataacacggc tgccagtggt gattgcgtta cggttacgat cgtaggtcgt
     2521 cataggtgtt ttctcctttc tgatttacag tcgaagccca cgtaacgggc gcaatataaa
     2581 tactcctgtc gagtgaaata ttttttgttt ttgatcaagc tcacatcttt tttgatgcat
     2641 gcatgagccg taaggcgggg ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa
     2701 ttactcttcg ctaaaccagt cgctgttttc ctggcggatc tggcgcacgg attcgctgat
     2761 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2821 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacat ggtcgaaact
     2881 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2941 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     3001 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     3061 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     3121 gataaaactg ccgttgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     3181 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     3241 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     3301 caggcaatgg acaatgtcgc gacgaagaat acgataaatc tgctgattga caggctgtgt
     3361 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     3421 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     3481 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     3541 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     3601 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     3661 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     3721 taccatgcag atccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3781 cgtaaacgtt accaccggca tacggccagc tgcctgccgt tgagccaatg ttaataatat
     3841 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat actaggcctt
     3901 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3961 tgcccaacgc caggccggca ttatttacca ggatatcaat attgctccac tcggcaggga
     4021 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     4081 gattatctcc cagttcgtct gttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     4141 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     4201 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     4261 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     4321 gcctacgcta tggaacacct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     4381 ttgtgcaaag cacactgccg tatctggctc ctcattttga tcaaattgcc aatcatcact
     4441 atcgcccggc attcgatgag ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc
     4501 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     4561 taattacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     4621 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     4681 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     4741 ttgatagtga atccatccgc ctggtggagg ttattcatca acgttttgtc cttgccggag
     4801 ccaaacttgc acaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4861 tgaccagcca gtttaaccag cggttacttg cagcaaataa atccggcggt ctggttgtga
     4921 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4981 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     5041 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     5101 cgcgagcgga aaaaaatgat gccaatgata cccgcgctat cattcaacgt ctggtagaga
     5161 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     5221 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     5281 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     5341 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     5401 aatttgatct tgatgaggcg cagctcaagc catattttga attaaacacg gtgttaaatg
     5461 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     5521 ttccggtcta ccatcctgac gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac
     5581 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     5641 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     5701 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     5761 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5821 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5881 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5941 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     6001 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     6061 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     6121 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     6181 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     6241 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     6301 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgt ggtaaggctc
     6361 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     6421 gcctggagat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     6481 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     6541 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     6601 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     6661 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     6721 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6781 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6841 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6901 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6961 tgttcactgt tttttgtcca cgctattata tgtaaaggtg atttgctttt tctgtatgct
     7021 cgtccaatat aataattttc aaacatataa atccgattat tctgaaaaca tgtttaagac
     7081 tcttcaatct tcttaacttt agtgagtcaa attctctgaa tatttgacag ctagtgggct
     7141 gattttaaat gccttataac aattggcagt attcttattt ggcaattata aaaatagcaa
     7201 ccagacttaa taagtcaaac caaacgcaat taaggagtta gtatgaaatt tcagtccatt
     7261 gtattagcaa gttttcttgt catgccttat gcgttggcag atgaccaggg cggtttaaaa
     7321 caggatgcag cgccaccgcc gcctcatgca atagaagatg gctatcgcgg aaccgatgat
     7381 gcaaaaaaaa tgaccgttga tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta
     7441 cgcggtaatt tgatttctca caaagg
//
LOCUS       DAJTRM010000155         7456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRM010000155 DAJTRM010000000
VERSION     DAJTRM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.155"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..695
                     /locus_tag="OO322_003821"
     CDS             <1..695
                     /locus_tag="OO322_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5908510.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="OO322_003822"
     CDS             complement(1034..1804)
                     /locus_tag="OO322_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908511.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="OO322_003823"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="OO322_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="OO322_003824"
     CDS             complement(2295..2516)
                     /locus_tag="OO322_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908512.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="OO322_003825"
     CDS             complement(2517..3200)
                     /locus_tag="OO322_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5908513.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="OO322_003826"
     CDS             complement(3264..3575)
                     /locus_tag="OO322_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908514.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="OO322_003827"
     CDS             complement(3572..4474)
                     /locus_tag="OO322_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5908515.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="OO322_003828"
     CDS             4914..5873
                     /locus_tag="OO322_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5908516.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="OO322_003829"
     CDS             5873..6268
                     /locus_tag="OO322_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5908517.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="OO322_003830"
     CDS             6451..6831
                     /locus_tag="OO322_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5908518.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6877..7456
                     /locus_tag="OO322_003831"
                     /pseudo
     CDS             6877..7456
                     /locus_tag="OO322_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2022 a         1930 c         1820 g         1684 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     6961 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccagt
     7021 gcaccatggc gcgatttacc cgaacgatat ggggcatgga aaactgttta taaccgcttt
     7081 aaccgatggt caaagtcagg cgtgattaat attattttca acaggttgct ttcgctactt
     7141 gatgcaaacg gctttattga ctggtctgcc accgcgctgg atggcagtaa tatccgggcg
     7201 ctgaaatgtg ccgccggtgc tcaaaaaaac atcccgatat cgacggagat aatgggccgg
     7261 gtcgctctcg cggcggtttt ggcaccaaaa tccatctggc aacagacgga agtggcctcc
     7321 cgttaaatat cgtgctaagc cccggacaag ctcatgaaag ccagttcgcg caacgacttc
     7381 tggacgggat tggcgttcag cgtcagaacg gcagcatgaa acgccgtgga catgcggtac
     7441 tggctgataa agcgta
//
LOCUS       DAJTRM010000156         7158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRM010000156 DAJTRM010000000
VERSION     DAJTRM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.156"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..678)
                     /gene="ospZ"
                     /locus_tag="OO322_003832"
                     /pseudo
     CDS             complement(1..678)
                     /gene="ospZ"
                     /locus_tag="OO322_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            734..886
                     /locus_tag="OO322_003833"
                     /pseudo
     CDS             734..886
                     /locus_tag="OO322_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2267..3214
                     /gene="icsP"
                     /locus_tag="OO322_003834"
     CDS             2267..3214
                     /gene="icsP"
                     /locus_tag="OO322_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5908519.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3776..4148)
                     /locus_tag="OO322_003835"
                     /pseudo
     CDS             complement(3776..4148)
                     /locus_tag="OO322_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4392..4511
                     /locus_tag="OO322_003836"
                     /pseudo
     CDS             4392..4511
                     /locus_tag="OO322_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4761..5627
                     /gene="ospB"
                     /locus_tag="OO322_003837"
     CDS             4761..5627
                     /gene="ospB"
                     /locus_tag="OO322_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5908520.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5956..6696
                     /gene="phoN2"
                     /locus_tag="OO322_003838"
     CDS             5956..6696
                     /gene="phoN2"
                     /locus_tag="OO322_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5908521.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7056..>7158)
                     /locus_tag="OO322_003839"
     CDS             complement(7056..>7158)
                     /locus_tag="OO322_003839"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5908522.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2238 a         1353 c         1382 g         2185 t
ORIGIN      
        1 cgagccttga tttgagaaag cctgtcattc atttgtttta tgtagctatc tatttcctct
       61 gatgcataac taaaaagttc tcccatttca cgagcaataa taatctcaga tttaatagac
      121 tttaatctct ggcgactcat ctcttaacac ttgcataatc tctcttttta tatcatatag
      181 ttctataaac cgatttgccc gtagttctcc ttgggaagaa tcatttctga atacaacaat
      241 tatatacaaa aaatcactat tttctttaga gaagatctta caactaatct ctgggagctc
      301 acataatgca tgtaagtcag gatggtacaa actatccaca gaaaattgcc ccataggacg
      361 ttttccagat ataactgtca accagcttcc ttccggtgta ctggcttcgg ctctggaaat
      421 aggctgaaaa tcttccggac atggagaata cattgtcttc atataattaa gtcgtctgga
      481 tttctcatta ataaactcct ctatttttaa ttgtgccata cttctggtta taccagagat
      541 atacttcttg tcatatatgt ttggtgattc attaaagtaa ccatgttcaa ctctaggata
      601 aatatttctc actatatatt cagtatttgc agtatttatt ggaaatacgt tcttaatatt
      661 cttgatggga ctaatcattt taatctctat actcaaatat aaacattacc atgaacaata
      721 taattgttcc taagtggaat gtctccacgg agaacacttt atctatcggg aaatagtgcg
      781 ggcaacagtg tttaattata tcgagtgcaa ttacaatcgc tggcgacggc accgttggtg
      841 tggcggcctc agtccggaac aatttgaaaa ccagaacctc gcttaggcct gtgtccatat
      901 tacgtgggta ggctcacaga gcctcactta cgactgcgtc catattatgt gggtaggatc
      961 aataaagacg ttttatatgt gtctgcttgc agccagaaaa tttataccct gcaggtatca
     1021 taaacatgct tgataactta attggggctc cctcttcctt ttatggtgta attgtttgat
     1081 tgaatacttc cggggatttc agtatgaaat gaagtatatt taatatactt tacaaaaagt
     1141 ttgtattagt ggaataagga caatagctta tccaaactgc caagcctctt tattataagt
     1201 aagatctggc actggaatat tctcaaaagg actaacatca tcaccacgac cttcaaccaa
     1261 ccattttatt gcatttaaat cccagaatag ttcagtagtg gtcaatttgg gaaacgcaca
     1321 atattgtacg gttacgttat cgcaatagag tgaactctcg ccctatacgg cgttttcaag
     1381 gattagtcct taatcggaca accacattaa agcataataa ataattaagc tttaataata
     1441 tactatagat acagttctat tataaaaaat actattttag gtgatgcatt aaattagtag
     1501 aatatttatt cttttataaa agcaccaatg aattgtccaa gtaaacatga gataaaatgt
     1561 gtagaggata actgagtttg gtcactttaa ctgtattagt cgcagtctga ctctcgactt
     1621 taaaaggatg ggaattacct gtactgatag tgagaaagct ttccattgaa ataagtcaaa
     1681 taaaagttga tggcttcagt gctaaagtga ggtgcccgct agccgtattc actcgaatcg
     1741 gaattcaatg tattgcttct gccatttcca taatatttca cgggaaaaac aaaatctgaa
     1801 aaactatact taagtgttgt attgagtcag ttgagataca aatacatatt tatcagcaca
     1861 cgttacgtca aatacaggag gttgtcatgt tggtactgaa aggcacgttg gctattgcag
     1921 ccgtgatgac aatttattgt cttatcgttg ttcttatgga acgcttttct gattgatttc
     1981 atattggcga gagaacaaga aggtgagtag aattgtcgcg gaatcctgaa actatcagcc
     2041 tcaggtatga acaccaaaag gtaggtagag aaatctatgg cccccgttac tttattcgca
     2101 caatgaggta tttttttatt tttacaaaag ttcaataaat attataataa aagatatgtt
     2161 cttggtaaca taaacaaata tttaattaaa ataatgcttg gcattgacgt ttatcttata
     2221 aagtaagaag atcatggaca tttcaaccaa aaaagtagag ttctcgatga aattaaaatt
     2281 ctttgtactt gcactttgtg tacctgcgat ctttactaca catgctacca ctaactatcc
     2341 acttttcata ccggacaaca tcagtacgga tattagtcta ggatctctga gtggcaaaac
     2401 aaaagaacgc gtttatcatc ccaaggaagg ggggagaaaa attagtcaac tggactggaa
     2461 atacagtaat gcgactattg ttagaggtgg catcgattgg aagctaattc caaaagtgtc
     2521 tttcggagtt tccggttgga ctactttagg taaccagaaa gcaagcatgg ttgataaaga
     2581 ctggaacaat tccaataccc ctcaggtatg gaccgaccag agctggcatc ccaatacgca
     2641 tctccgtgat gctaacgaat tcgagctgaa tcttaaaggt tggttattaa ataatttgga
     2701 ttatcgactt ggactaatag caggttacca agagagtcgt tacagtttta atgcaatggg
     2761 agggagttat atttatagtg aaaacggggg gagcaggaat aaaaaagggg cacatcctag
     2821 tggtgaaaga acaataggtt acaaacagct cttcaaaata ccttatattg gattaactgc
     2881 taattaccgc catgagaatt ttgagtttgg agcagaactg aaatatagtg gttgggttct
     2941 ttcatctgat acagataaac actatcagac tgagacaatt tttaaagatg aaataaaaaa
     3001 ccaaaattac tgctctgttg ctgcgaatat tggatactat gtcaccccca gtgcaaaatt
     3061 ttatatagaa ggctccagaa attacatttc taataaaaaa ggtgatacat ctctttatga
     3121 gcaaagtacc aatatatctg gcaccattaa aaatagtgca agtattgaat atattggttt
     3181 tctcacttcc gcaggtataa agtatatttt ttgaattggt tcaccgagtt gattacgttt
     3241 tcatggctaa aaattgccgc ccattgtgag tcatattcgc cctgactttc cagaaccata
     3301 cgaactgccc gttgacggac ttcgggggaa aaacgtgtat ttttagtcat tctgtttacc
     3361 tctttctcag ggcgtttagt ctccaggatt tccggggcgg ttcaaaaaac tcttcgttga
     3421 ttactgtgga cttactgttg gcattaccga ccctgagacc ggagaaataa gaaccgctta
     3481 gatcatcgtg gctgttctcg gggtatcaag ttacacatgg gcagaggcta cccaaggtct
     3541 actattatcg cctgaaagcg ctgatggaac agtgctatca gggacatgct gatgaaaata
     3601 cctaaataat cagccagcac tctatctttc caaatccaca gcatagcaaa gagagcaaaa
     3661 gagcctgtaa attcagaaat taaggaaacg cctcttggtg tacataatct atcttatgtt
     3721 aaatgaaatc taatttcttg attatatgct ggatcgttcg tgggtggctt tgcccctata
     3781 tttccagaca cctgttatca ttggcccgct gccgcagata ttcccgtggc gagcgataat
     3841 ccagcgcact atgcggatgc cattcgttat aatgctcgaa tgcctctgca aggttctttg
     3901 ctgccgttaa cccgtcaggt ttgggcatga tactgatgta gtcacacttt attgttttca
     3961 cgaagctctt tgctattctg ttactctccg gactccgcac cgccgtgttc ttcggttcaa
     4021 caaagtccca acatccgggt gaactggcgt gtttcattag ctcggtagca tgaaccatta
     4081 tccgtcagtc acttcactgg agacgccgga agctcgttgc cgaagcggtg ttccaccgct
     4141 cccagcatgt cgccaacggg agagcatcta cggtctgatt gaactgttta ttcaaattgt
     4201 ccaccgtctg tcagtacgct ctgaacgtcg gctggttaaa acactgctcg ctgattttca
     4261 gagggttcac ggtaaaacag cacttttatt ccggattgcg gaagcggctc tgaataatcc
     4321 ggatgggctg gttaaagagg ttgtgtacca tctgcatatt gttgaaccag atcgcagcgg
     4381 taagagaagt tcatcatact tggcgcagtt acgcgatgtt tctgctagag gtgatgccgt
     4441 aaagaacggc agaacattac cggaacaaga tagcggtcta cctgcactgg tatcagaccc
     4501 ggggcttccc cggatgatct ccacggtact ttaggtactg tgtctatatt atgtaggtag
     4561 gatcaattaa ctttgtagtt gaaattcttt cattgaaata atgaataaaa aagacaaacc
     4621 gcattaaaca atgccagaat ataattgcat ttttgttccg tttttttaaa tcatagtata
     4681 caaagaagct tgcatactat atgaaaatat aacacccttt actatgatgt ttataaaaca
     4741 atatatggag tcatgtaggt atgaatttag atggtgttag accatactgt agaatagtca
     4801 ataaaaagaa tgaaagcata tcagatattg catttgcaca tataataaaa agggtaaaaa
     4861 attcatcatg tactcaccca aaagcagcat tggttttttt aggagagaaa ggtttttgtg
     4921 atagcaatga tgttctatct attatgggac aacaaatacc aagagtattt aagaacaaga
     4981 tgttatatga ttatgttttt aaaaatgaaa aaagtaaaaa tgattttcta aaaatggctg
     5041 aatcatggct accacagagt gaaccaatag taataaataa tgatgatgac gcattgaatg
     5101 ctgctgctta tttttctgta aaaaaagcga aaataaaaac agtaaacgat actgatttta
     5161 aagagtataa taaggtttat attcttgggc acggtagtcc tggttctcat caattaggcc
     5221 ttggttcgga acttattgat gtacaaacaa tcatttcaag aatgaaagac tgtggtattc
     5281 taaatgtgaa agatatccgt tttacttcat gcggctccgc tgataaagtg gctcctaaaa
     5341 attttaacaa tgcccctgct gaaagtcttt cttgtatcct taactctctg ccttttttta
     5401 aggaaaaaga atctttgcta gagcagataa aaaaacacct tgaaaacgat gagtcattga
     5461 gtgatggtct aaaaatatcc ggctatcatg gatatggagt tcactatggt caagagcttt
     5521 ttccctactc acattatcgt tcaacttcaa ttcctgctga tccggagcat acagtaaaaa
     5581 gaagctctca gaaaaagact tttattatta ataaagaact ggattagtat aaaattttta
     5641 acctatagtt aattgtttgc atttaagatg aagttatttt atctttagta gaaaagtgct
     5701 tccctatgag ggaaaatcac aaatcatcat aatacagaga caaaacgata cgaaaaaata
     5761 gataaaaaac atcattcttt taccacatta ttttccgtga atatgaaaaa taatgttatt
     5821 actttaatat aagactattt tttgtttttc catcactctg ttcaaatttt tccgcatgac
     5881 ttgtgttttt tgtaatacag ctcgtttttt acagctgacc aaaatcatca attaattatg
     5941 ctaaggaaat aaattatgaa aaccaaaaac tttcttcttt tttgtattgc tacaaatatg
     6001 atttttatcc cctcagcaaa tgctctgaag gcagaaggtt ttctcactca acaaacttca
     6061 ccagacagtt tgtcaatact tccgccgcct ccggcagaga attcagtagt atttcaggct
     6121 gacaaagctc attatgaatt cggccgctcg ctccgggatg ctaatcgtgt acgtctcgct
     6181 agcgaagatg catactacga gaattttggt cttgcatttt cagatgctta tggcatggat
     6241 atttcaaggg aaaatacccc aatcttatat cagttgttaa cacaagtact acaggatagc
     6301 catgattacg ccgtgcgtaa cgccaaagaa tattataaaa gagttcgtcc attcgttatt
     6361 tataaagacg caacctgtac acctgataaa gatgagaaaa tggctatcac tggctcttat
     6421 ccctctggtc atgcatcctt tggttgggca gtagcactga tacttgcgga gattaatcct
     6481 caacgtaaag cggaaatact tcgacgtgga tatgagtttg gagaaagtcg ggtcatctgc
     6541 ggtgcgcatt ggcaaagcga tgtagaggct gggcgtttaa tgggagcatc ggttgttgca
     6601 gtacttcata atacacctga atttaccaaa agccttagcg aagccaaaaa agagtttgaa
     6661 gaattaaata ctcctaccaa tgaactgacc ccataaagct ggacagcctg tatcaggcta
     6721 tggagggccc atagacaaat ctaccctata tgagcatagg aggagtctat gggcacacca
     6781 cgttttaccc ctgaatttaa gggattactg gaaaggctgg gacatatccc tccggcagaa
     6841 gcagaaaaag cttattatgc tgccatcgga aacgatgatc tggcaacctg agttcacaga
     6901 taaaacattc tctaggaaac tcggggcggt tccgttcacc acatgcaatg tggtgttgca
     6961 ggggaacggt ctgcaccatc ccctatgtcg atcaatataa cagaaatgac aacttcagat
     7021 tcagggcaca acctaaatat attttaggtc acctctcaaa tcgtttgcct gataccgctc
     7081 cattttttaa taaaaaaatc aatcattttt gaatccttgc ttttcattgc attgtcaagc
     7141 atagtgtccc cactattt
//
LOCUS       DAJTRM010000157         7045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRM010000157 DAJTRM010000000
VERSION     DAJTRM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.157"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(159..1061)
                     /locus_tag="OO322_003840"
     CDS             complement(159..1061)
                     /locus_tag="OO322_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5908523.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1140..2093)
                     /gene="lsrR"
                     /locus_tag="OO322_003841"
     CDS             complement(1140..2093)
                     /gene="lsrR"
                     /locus_tag="OO322_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5908524.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2342..3877
                     /gene="lsrA"
                     /locus_tag="OO322_003842"
     CDS             2342..3877
                     /gene="lsrA"
                     /locus_tag="OO322_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5908525.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3871..4899
                     /gene="lsrC"
                     /locus_tag="OO322_003843"
     CDS             3871..4899
                     /gene="lsrC"
                     /locus_tag="OO322_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5908526.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4899..5891
                     /gene="lsrD"
                     /locus_tag="OO322_003844"
     CDS             4899..5891
                     /gene="lsrD"
                     /locus_tag="OO322_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5908527.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5903..6926
                     /gene="lsrB"
                     /locus_tag="OO322_003845"
                     /pseudo
     CDS             5903..6926
                     /gene="lsrB"
                     /locus_tag="OO322_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            6953..>7045
                     /locus_tag="OO322_003846"
     CDS             6953..>7045
                     /locus_tag="OO322_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5908528.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1636 a         1781 c         1842 g         1786 t
ORIGIN      
        1 ccagaaaagc acttcgcatc actcagacat gatttaacat aatatacatt atgcgtagta
       61 atattgtagt agctaaattg agatgtccgt ctctgactaa atagacatgt ccgcgctaca
      121 gtctcaccac agcgatcact gctttcaaag gatgggactc atgacggcat acggaacgga
      181 gtaatcggca actgatgccg ggcctcgctg agcgtgcact gcttattatc cgtgaacgtg
      241 ccgccaagaa ccgcggtttg tgccggacgc acaacgccta acccaaggca accaagctgc
      301 acgtcgcctc ctccaacgac caccagagtg cccgccttca gaccgcagag ttccgccgct
      361 tgtgaactta ccacgcccag caatgtgccg gtttctttga caggagaaag aatatcggca
      421 cgcaggccag ccatatccag caatgcaggt ttccaggcac gggtggttag atcaagaagt
      481 cccgtggtgc cagcgttaga gggatccacc gccagttcgc cgctgagcat ataggccagc
      541 cagtcgctga tcatggtgat ggttgatgcc tgacggtaaa tatcggaacg atggtgcgcc
      601 agccaaagta atctggggat ggcacttaaa gccagtgttt gtccggtcgc tcgataaact
      661 tcgttttcaa aggtattgtt gtgcagttct ttaagttcgc taacttcgcg tgccgctctg
      721 gcatccacat tggcgcaggc ccagatcggt gttccttcat tgttatataa aacaatgcct
      781 tcacgcatag aacatgccga aacggcagcg atatactccg ggtctatgcc ggcgttgtgc
      841 agcgcctggc gcatacactc acacgccagt tgccagtttt tgttgagatc aaattccata
      901 gaaccaggaa cgtccggtac tgccagatgc cgccactccg cctgtcccac tgctatttga
      961 ttgccttcca ggtcgaatat cacagcccga atacttccgg tgcctgcatc cagcgccatc
     1021 aggtagtact ttgattctga aggggtaaag agtcgagcca ttagattatc ctcggcttat
     1081 gcgttctgtc tgttcctcta tacgttctcc atcattcccg gtaataaggt catgcaaatt
     1141 taactacgta aaatcgccgc tgctgtgtcc tgatcggtaa ccagtgcgtt gatataaccg
     1201 cctttcattg cagcggcaat tgcttcagct ttattttctc cccctgccac gccaacccgg
     1261 acgggtatgg tcttcagcgc gcttaaaggt aagccaatca gttcgttatg tattttgata
     1321 tccgtgacaa cgtcaccttt tgcatcaaaa aagtagccta aaatgtcgcc aaccgcccct
     1381 tttcggccaa tcattaactg ttcgccctgg ctgatataac cggagcgaat gattgtcgcg
     1441 tcgtcttgtt gactcacggc tccaatgccg acaatcgcca catccgctgc ttgcgcggct
     1501 agcagaacat ctttgacgca attttcattt tttagcgtac gggcaatgtc agcggaggat
     1561 gcccgcaacg gagccggaat aatattcaca ctgcacgccg cgttaagctg cccgataccc
     1621 gtcatataag aaccgacgcc accggagagc gtgaccaggc gaatttgctg tgacgaaata
     1681 aaaccactta agcgttgcag cgtattcatg gttgcctcgc caaaaccaat cgccagcatc
     1741 tgttgtggtt gaagtaaact catcaacata tgcgccgcgc ctatccctag tcgcccgccg
     1801 acatcagcat ccgcaagccc agggatcacc cggacatgtt gcagcgaaaa ctgacgacgt
     1861 aattgagttt catattccag acagccttca aagcgagaat taatctgtac gcgaataatg
     1921 ccggactgat gccctttctc cagcaagcgc gacactttca aacgggtcag gccgagacga
     1981 tcgctgatct cgctctgggt cagcccgtcg tgatagtaaa accacgcgat ccgcgcgacc
     2041 tgttcttctt cacacattcc ctgttctgaa attgccgaat cgttgattgt cataattcat
     2101 tcttcacttt gaacatattt aaatctttaa tgcaattgtt cagttcttgc gcatttatat
     2161 ctgtgatggc aaccacagtt tgactctacg agcatgaaca aacgcaaccg tgaaaatcaa
     2221 aatagcataa attgtgatct attcgtcgga aatatgtgca atgtccacct aaggttatga
     2281 acaaattaaa agtagaaata catttgttca aaactcacct gcaaaactga acgggggaaa
     2341 tatgcaaacg agtgataccc gcgcgttacc gctactttgc gcccgctcgg tttataaaca
     2401 gtattcaggg gtcaatgtcc tgaaaggcat cgattttacg ttgcatcagg gggaggtcca
     2461 cgccctgctc ggcggcaatg gtgccggtaa atcgacgtta atgaagatta ttgccggtat
     2521 tacccctgct gatagcggta cgctggagat tgggggcaac aactacgcca gattaacgcc
     2581 agttcatgct catcagctgg gcatttatct cgttccccag gaaccgctgc ttttcccaag
     2641 cctgtcgata aaagaaaaca tcctgtttgg gctggcaaaa aaacagctct ccatgcagaa
     2701 aatgaagaac ttgctggcgg cgctgggctg ccagtttgat ctgcatagtc tggcaggatc
     2761 gctggatgtc gccgatcgcc aaatggtgga aatcctccgc gggctgatgc gcgactcgcg
     2821 gattctgatc ctcgatgaac ctaccgcctc gcttacccct gcggaaaccg aacgcttgtt
     2881 tagtcgcttg caagagctgc ttgctactgg cgtgggtatt gtttttatct cgcataagct
     2941 gccggaaatt cgccagattg ccgatcgaat tagcgtgatg cgcgacggaa ccatcgcctt
     3001 aagcggcaaa accagcgaac tgtctaccga cgacattatt caggccatca ccccagcggt
     3061 acgggaaaaa tcgctctctg ccagccaaaa attatggctg gagttacctg gtaaccgccc
     3121 acaacatgcc gtcggaacgc cggtgctgac actggaaaat ctgactggcg aaggcttcag
     3181 gaatgtcagc ctgacgctca atgccggaga aattctgggc ctggctgggc tggtgggagc
     3241 cggacgcaca gaactggccg agacgctcta tggtctgcgt actttgcgtg gcggacgcat
     3301 tatgctgaat ggtaaagaga tcaataaatt atccactgga gaacgtttac tgcgcggtct
     3361 ggtttatctg ccggaagatc gccagtcatc cggactgaat ctcgatgctt cgctggcctg
     3421 gaacgtctgc gcccttactc ataaccttcg tggattctgg gcgaaaaccg cgaaagataa
     3481 tgccaccctg gaacgttatc gtcgggcgct gaatattaaa tttaaccaac cggaacaagc
     3541 tgcacggaca ttatccggta gcaaccagca aaaaatcctc attgccaaat gcctggaagc
     3601 ttcgccgcaa gcattgattg tcgatgagcc gacgcgcggc gtggatgtct cggcccgtaa
     3661 tgatatctac cagctgttgc gcagcatcgc cgcacagaat gtggctgtgc tgtttatctc
     3721 ctctgatatg gaagagatcg aactgatggc agatcgtgtg tatgtgatgc atcagggcga
     3781 aattacccac tctgcactga ccgggcgcga tattaatgtc gagactatta tgcgcgttgc
     3841 cttcggcgat agtcagcgtc aggaggcatc atgctgaagt ttattcagaa caaccgtgaa
     3901 atcacggcat tgctggcggt ggtgctgctg tttgtattac ccggttttct cgaccgccag
     3961 tatttaagtg tgcaaacgct gaccatggtt tatagcagcg cgcaaatcct gatcctgctg
     4021 gcaatgggcg cgacgctggt aatgcttacg cgcaatattg atgtttcagt gggttcgatt
     4081 accggaatgt gcgcggtgct gttggggatg ttactgaacg caggatattc actacctgtt
     4141 gcctgtgtcg cgactttact gcttggtttg ctcgcgggat ttttcaacgg tgccctggtc
     4201 gcgtggctaa agatccctgc cattgttgcc acccttggca cgttagggtt gtacagaggc
     4261 atcatgttgc tgtggactgg cggtaaatgg attgaagggt tacccgccga actgaaacag
     4321 ctctccgccc cgctgctgct tggcatttca gcaattggtt ggttgacgat aattctggtg
     4381 gcatttatgg cctggctgct ggcgaagacg gcgtttggac gcagttttta tgccacgggc
     4441 gataatttac agggcgctcg tcaactgggc gttcgtacgg aagccattcg cattgtggca
     4501 ttttcgttga acggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt
     4561 tttatcctca accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg
     4621 ggcggcatta gtttgctcgg tggttccggt gcgatcatcg gtgcggtact cggcgcatgg
     4681 ttcctgacgc agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaatgat
     4741 tttatcgcgg gtctggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg
     4801 ctggaacgta atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt
     4861 aaacccgctt cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg
     4921 aactggctct tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc
     4981 gaatgttaga tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg
     5041 tcgctctacc gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga
     5101 ccatcggcct ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc
     5161 tggcgatact cctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa
     5221 ttatctatac caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg
     5281 gaagcgctct gctgctttcc ggtatggccg gagcgacggg gtacgaaggt attggtggat
     5341 tcccgatggc gtttacagat ttcgctaacc tggatgtgct gggactcccc gttccgctga
     5401 ttatcttcct gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac
     5461 gtaatgtgtt tttgattggg caaagcccgc gcgtggcgct ttatagcgcg attccagtta
     5521 accgcacctt atgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc
     5581 tgctggtatc gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg
     5641 ccatcaccgc cgtggtgctt ggcggggcca atatttatgg tggttccggt tccattatcg
     5701 gcaccgccat tgcggtttta ttagtgggat atttgcagca aggtttgcaa atggcaggag
     5761 tgccaaatca ggtgtccagc gccctttccg gtgcgctgct tatcgtcgtt gtcgtaggtc
     5821 gttccgttag cctgcatcgc cagcaaatta aagagtggct ggcgcgtcgg gccaataacc
     5881 cattgccata aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttacttag
     5941 cgctcttggc attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat
     6001 tcccaaactg gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg
     6061 taaagagctg agcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca
     6121 ggtacagttg attaataact tcgtcaatca aggttataac gccattattc gtttctgcgg
     6181 tttcgcctga tggcttgtgt ccggcactga aacgcgccat gcaacgtggt gtgagagtgc
     6241 tgacctggga ctctgatact aaaccggagt gccgctctta ctacattaat cagggaacgc
     6301 ccgcccagtt gggaggtatg ttggtggata tggcggcgcg tcaggtgaat aaagacaaag
     6361 ccaaagtcgc gtttttctac tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag
     6421 aagcgaaagc gaaaatcgcc aaagagcatc caggctggga aattgtcact acgcagtttg
     6481 gctataacga tgccactaaa tcgttacaaa ccgcagaagg aatattaaaa gcgtatagcg
     6541 atctcgacgc cattatcgcc cccgatgcca acgccctgcc cgctgccgca caagccgcag
     6601 aaaacctgaa aaatgacaaa gtagcgattg tcggattcag tacgccaaat gtgatgcgcc
     6661 cgtatgtaga gcgcggcacg gtgaaagaat ttggactgtg ggatgtggtt cagcaaggca
     6721 aaatttcagt gtatgtcgcg gatgtattat tgaaaaaagg atcaatgaaa acgggcgaca
     6781 agctggatat ccagggcgta ggtcaggttg aagtctcgcc aaacagcgtt cagggctatg
     6841 actacgaagc ggatggtaat ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag
     6901 agaatatcgg caaatacgat ttctgatgtg cattacttaa ccggagtaag ttatggcaga
     6961 tttagacgat attaaagatg gtaaagattt tcgtaccgat caaccgcaac aaaatatccc
     7021 ttttaccctg aaaggttgcg gtgcg
//
LOCUS       DAJTRM010000158         7022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRM010000158 DAJTRM010000000
VERSION     DAJTRM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.158"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            41..1531
                     /locus_tag="OO322_003847"
     CDS             41..1531
                     /locus_tag="OO322_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5908529.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1583..2740
                     /locus_tag="OO322_003848"
     CDS             1583..2740
                     /locus_tag="OO322_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5908530.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2706..2828)
                     /locus_tag="OO322_003849"
     CDS             complement(2706..2828)
                     /locus_tag="OO322_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908531.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3031..4716
                     /locus_tag="OO322_003850"
                     /pseudo
     CDS             3031..4716
                     /locus_tag="OO322_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4754..>7022
                     /locus_tag="OO322_003851"
     CDS             4754..>7022
                     /locus_tag="OO322_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5908532.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVL"
BASE COUNT         2074 a         1533 c         1485 g         1930 t
ORIGIN      
        1 atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc ttggttatgg
       61 acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc gtgaagttgt
      121 cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat caacgccgac
      181 gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg cacacggtgt
      241 ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct ttggtgtcta
      301 ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc ctgaattatt
      361 ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa aaatcagtaa
      421 tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca ccacattttc
      481 tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat tccacgctgc
      541 aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa
      601 aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc gtgccaaaac
      661 acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac caaatgataa
      721 tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag cagataacta
      781 ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa
      841 aaacggacag tatgacaata caattattct ctttacctcc gataatggtg cggttatcga
      901 tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct atcctggcgg
      961 tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta attatgacaa
     1021 gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata tcagcattcc
     1081 aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata agaaacaagg
     1141 cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg atgaggaaaa
     1201 tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg attacccgca
     1261 taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata acgattattc
     1321 gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg atctacagca
     1381 aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag
     1441 agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga agtttaacaa
     1501 tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg atttttccgc
     1561 cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa
     1621 tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc atgaaaaatg
     1681 gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt ctggcaatca
     1741 ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt ttttccgtaa
     1801 agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata cattacaaac
     1861 gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg aatttctggt
     1921 tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca gtaattcagg
     1981 taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat atcaagtaga
     2041 gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg aggtttatca
     2101 ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag aaaccgggac
     2161 gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg atttttctgt
     2221 gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg ttaaaaacga
     2281 tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt tggggaatgg
     2341 gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg aaagtaatgg
     2401 agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa acattaataa
     2461 atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac ggatttcagc
     2521 gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta agcatcgtat
     2581 tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa tccttttttc
     2641 aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag taccgcttta
     2701 ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc ttttaactcc
     2761 attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc tgtcgggtgt
     2821 ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg tcagggcatt
     2881 agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa aatttctata
     2941 tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt gccatagata
     3001 atcattggat ggcacaatca attttcgcta atgataacca ttcccattac gttttgtatg
     3061 ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact
     3121 tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa aattcaggtc
     3181 tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag
     3241 ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt aatatccgca
     3301 aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac
     3361 taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat
     3421 aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcaacaaa
     3481 acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt
     3541 attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc
     3601 caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt atttacccat
     3661 aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt
     3721 cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag caatgctgag
     3781 tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt
     3841 ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc
     3901 gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga
     3961 ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg gtttatttat
     4021 aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga gttccatcaa
     4081 ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt acaagtggct
     4141 aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa ctttcatgtt
     4201 tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac cacactgctt
     4261 aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc tgctgacagt
     4321 tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat tatttgtaaa
     4381 gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca ggttggtctg
     4441 ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct ttccagcggc
     4501 gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg gatatttctt
     4561 gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg tttagtgcgt
     4621 gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt ctggaacctg
     4681 gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc ctgctgtacg
     4741 gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg gcgctgatgt
     4801 ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga tgacggtcta
     4861 tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa gcatcgagcg
     4921 tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg cggtacgcat
     4981 ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga aaatctctat
     5041 tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg caactaataa
     5101 cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg ggatgtcaca
     5161 ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca gttttgtgcc
     5221 ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac gcttcaacgc
     5281 tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt taacatcgca
     5341 tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta cctatttctc
     5401 tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg ctgataactt
     5461 tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg attatgtttc
     5521 gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata ctgcattgag
     5581 caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa aatataccgg
     5641 ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa
     5701 atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac tacgtaccac
     5761 cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct ggtacaccga
     5821 actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat taggacacat
     5881 ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa aattcgccgt
     5941 gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg atgcatggac
     6001 tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga aaactaacca
     6061 taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc tgtatatggc
     6121 ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg actatgacaa
     6181 ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata tttttgctga
     6241 tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata ttcttgatat
     6301 gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata aaaccctgac
     6361 gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat tgcagcaaaa
     6421 aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca gcaaaagcag
     6481 tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg gcaaaaccaa
     6541 aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc gccaggtaga
     6601 tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa
     6661 tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta atctggtctc
     6721 ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct taaacatgga
     6781 tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg
     6841 taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat attcagatta
     6901 caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc gcttgtcctg
     6961 gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc tcaatgtact
     7021 gg
//
LOCUS       DAJTRM010000159         6693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTRM010000159 DAJTRM010000000
VERSION     DAJTRM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.159"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1410
                     /gene="sbcB"
                     /locus_tag="OO322_003852"
     CDS             <1..1410
                     /gene="sbcB"
                     /locus_tag="OO322_003852"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5908533.1"
                     /translation="KQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFY
                     CKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFD
                     DEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRL
                     EHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVP
                     QMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDIDTLRER
                     LYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILREN
                     PQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITF
                     VDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLVQQYAD
                     DKEKVALLKALWQYAEEIV"
     gene            complement(1453..1680)
                     /locus_tag="OO322_003853"
     CDS             complement(1453..1680)
                     /locus_tag="OO322_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5908534.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1694..2752)
                     /gene="yeeE"
                     /locus_tag="OO322_003854"
     CDS             complement(1694..2752)
                     /gene="yeeE"
                     /locus_tag="OO322_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5908535.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2931..4289)
                     /gene="plaP"
                     /locus_tag="OO322_003855"
     CDS             complement(2931..4289)
                     /gene="plaP"
                     /locus_tag="OO322_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5908536.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4279..4341)
                     /gene="yoeI"
                     /locus_tag="OO322_003856"
     CDS             complement(4279..4341)
                     /gene="yoeI"
                     /locus_tag="OO322_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5908537.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4502..5485)
                     /locus_tag="OO322_003857"
     CDS             complement(4502..5485)
                     /locus_tag="OO322_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5908538.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5531..6355)
                     /locus_tag="OO322_003858"
     CDS             complement(5531..6355)
                     /locus_tag="OO322_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5908539.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6438..6692)
                     /gene="yoeB"
                     /locus_tag="OO322_003859"
     CDS             complement(6438..6692)
                     /gene="yoeB"
                     /locus_tag="OO322_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5908540.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1799 a         1764 c         1571 g         1559 t
ORIGIN      
        1 aaacaaccta ccttcctctt tcacgattac gaaacctttg gcacgcaccc cgcgttagat
       61 cgccctgcac agttcgcagc cattcgcacc gatgacgaat tcaatgtcat cggcgaaccc
      121 gaagtctttt actgcaagcc cgcggatgac tatttacccc ggcctggagc agtattaatt
      181 accggtatta ccccgcagga ggcacgggcg aaaggagaaa acgaagccgc gtttgccgcc
      241 cgtattcact cgctttttac cgtaccgaag acctgtattc tgggctacaa caatgtgcgt
      301 ttcgacgacg aagtcacacg caacgttttt tatcgtaatt tctacgatcc ttacgcctgg
      361 agctggcagc atgataactc gcgctgggat ttactggatg ttatgcgtgc ctgttatgcc
      421 ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg gtctaccgag ctttcgcctt
      481 gagcatttaa ccaaagcgaa tggtattgaa catagcaacg cccacgatgc gatggctgat
      541 gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc gtcagccacg cctgtttgat
      601 tatctcttta cccatcgtaa taaacacaaa ctgatggcgt tgattgatgt tccgcagatg
      661 aaacccctgg ctcacgtttc cggaatgttt ggggcatggc gtggcaatac cagctgggtg
      721 gcaccgctgg cgtggcatcc tgaaaatcgc aatgccgtaa ttatggcgga tttggcagga
      781 gacatttcgc cattactgga gctggatatc gacacattgc gcgagcgttt atataccgca
      841 aaagccgatc ttggcgataa cgccgccgtt ccggttaagc tggtgcatat caataaatgt
      901 ccggtactgg cccaggcgaa tacgctacgc ccggaagatg ccgaccgact gggaattaat
      961 cgtcagcatt gcctcgataa cctaaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa
     1021 gtggtagcga tattcgcgga agccgaaccg tttacacctt cagataatgt ggatgcacag
     1081 ctttataatg gctttttcag tgacgccgat cgtgcagcaa tgaaaattgt gctggaaacc
     1141 gagccgcgta atttaccggc actggatatc acttttgttg ataaacgaat tgaaaagttg
     1201 ttgttcaatt atcgggcacg caacttcccg gggacgctgg attatgccga gcagcaacgc
     1261 tggctggagc accgccacca ggtcttcacg ccagagtttt tgcagggtta tgctgatgaa
     1321 ttgcagatgc tggtacaaca atatgccgat gacaaagaga aagtggcgct gttaaaagca
     1381 ctttggcagt acgcggaaga gatcgtctaa tttgaagccg ccgccgctgg tacggcggag
     1441 gacaatgttg agtcaggctt tttgaacggt gatgctccac gctgcatcgc caatctgctg
     1501 ataatcggtg atggcatgtc cttcttctgc cgcccactgt gggattgctt cagttgcctg
     1561 ggtgcaatca aactcaatta ccaactcatc gccgctggcc atttctgcca gtgctgcttt
     1621 tgcttctatg agtggaaacg gacagacctg cgtcaccaca tccagctttt taatcgccat
     1681 ctttacacct tacttaattt gccgcagcag ttgccagtcg cgccttacgc tgcggtcgaa
     1741 catacacaag ccaggacgca gtccagactc cgagaatcat aaatacaagg ccaatccagc
     1801 cctgccaggt catcattgca gtcataacca gtccattacc gatagagcaa ccacctgcaa
     1861 tactggcacc gaagcccatc agtacaccac ctaacccgct acgtaatgtt gtttgtgcat
     1921 cagctgcgcg aacgcggaac tcacggctcg ctttggctgc aataaatgcc tccacgaaga
     1981 tccctaacac caggaaaacg ccccagttaa tgtatttcac gtcacccgcg accagaaatt
     2041 gaagaatatt ggccgttggg gaagtgactc ctaacccaaa catgcgtccg gttgcttcac
     2101 tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac agcagtgaca aagggatgcc
     2161 agcgtttttc aaacagaata tgagcgatcc cggttcggcg cggcggtaag gtcgcgactt
     2221 tgagttttgg cttcttcaac tctttcatca ccacccagag cgttatcacc agcaaaacgg
     2281 taaccaacgg ccacacagac aaattaaaag tctcagcaat agagttatgt tcagtagtgt
     2341 agtgctgcaa ggtttgattt aaaccactgg cgtgtggaga acgcatcacc gcactcatca
     2401 ccatataagt gaaaagcgcg atccaactgc cgatcaatcc ctcacccgcg cgataccagg
     2461 tcccggtggc acatccaccc gccagaacaa ttcccagccc gaaaagatac ccacctataa
     2521 cagtaccaag ccacgggaac gccccggctt cgtaagtcag taggcccgcc tgaattaacg
     2581 caaaaacccc cacgctttgt gccgaaattg caatcagcaa ggcgtaaaac atgcgattat
     2641 ttttcgcgat atacatatcg cgaaaaccac cagtcaggca aaaacgccca cgctgcatga
     2701 caaatcccag cagagcacca caaattagcc cgcttaatat cattgaaaac ataaattaat
     2761 taaccagatg aatgttaatg gggaaatttt tcatgactgg tggaatgcag accaataacc
     2821 aaattctcta aattagaaca aatggttatt aatgaggatt tcgataaaaa agaataaaaa
     2881 aaccggagcc aaatgttcat tcgactccgg tttattatta gaaaagatga ttatgctacg
     2941 tcttcgtact gcggaaccgg attacggaag ctcttggtga cgcaagccag gtaaatcaga
     3001 ccgatagcag cccagatcag acccagaacc attgagcttt cttccaggtt aacccacagc
     3061 gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg atctttcagc
     3121 gtcttgttac gcttctcacg gatccagaac tgcgagatga ccgacaggtt aacaaaggtg
     3181 aacgccacca gcgcaccaaa gttaatcagc gccgttgcca ttaccaggtc gaagttgatt
     3241 gccagcaggg caatcgcgcc aaccaggatg atgttcatcg ccggagtacg ccatttcggg
     3301 tgtacataac cgaagaagct tttcgggaac acgccgtcac gacccattac gtacatcaga
     3361 cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt ggagaagatc
     3421 agcgcgccaa cctggaacgc tttacccgca acgtacagca tgatttcagg ctgtgacgca
     3481 tccggatctt taaagcgaga gatatccggg aagtacagct gcaggaagta agttgcaaag
     3541 atgaagatca tgccgccaat cagcgcggtc aggaaaatcg cacgcgggat cacgcgctct
     3601 gcatctttgg tttcttccga cagattgctg atgccgtcaa agccggtaaa ggagaagcac
     3661 aggattgtcg ccccggtaat catcgggata acatgcgcat cgccagacca gaacggacgc
     3721 gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac catgcccaga
     3781 atcaccgcga tcagcactac ctgcaacacg acgattacag tgttgacgtt cgctacggat
     3841 ttcagactac gcaggttaaa ggcggtcata aaggccacca gcgccaccac aaacatccac
     3901 gatggaatgg aaggcaccag agcttcaaaa tagattttcg ccagcagaat gttgatcatc
     3961 ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac agtcgggcta
     4021 atggatttct gggcgtaagt gtatgcagag ccagcagaag gatagcggcg aaccagcttc
     4081 ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc tgtcggcaca
     4141 tgaccatccg tgaggcctga aacgatacca aatgtatcaa acagcgtcat cggctgcata
     4201 taggctagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa ttccacgcga
     4261 gaggtgtttg gagtaacgtt atgcgacatg gttattctcc tttacggtga taaccgtcgc
     4321 gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg tcgattttta
     4381 gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt ttgaagcaaa
     4441 aaataaccga cgctaattaa acgtcggttt ttaatcattt tttgacagcg gcgcattgtg
     4501 cctaaatggg ggggaaatga caagagaatg agaggcttgt cataataatt tttctttgaa
     4561 tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta tttgctaaaa
     4621 tttcatctcg ccaccagccg ctggcaagcc ctgcggtctg ttcattccag ccaatccaga
     4681 caggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca ctatcgagaa
     4741 aacgttgcgc cagataccgt ggtaaatagc cgcagccaag gccgctaatt tgcagctcca
     4801 gtttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt tgtgaggccg
     4861 tagaagcgcc tgcctgcgca gtatccccca ccacaatcgc ccgatatcgt ttaatgatac
     4921 gacggtttaa cggttcttct tcctgggcta acggatgatg aggcgcgacc gcaaagactt
     4981 gttcaagatc gcccaaccgc gagaaaccaa actcactgga agatggtggc tcatgcatcg
     5041 cgccaacgat aatatccgct cgcccctgag tcagggcatc ccaggaacca ccgagtacgc
     5101 cattgataaa cttcaggcgc gtcacgctat gatgttgata aaaagcttca atgagaggcg
     5161 caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt tcccagcctt
     5221 catgcagttt gatcgcctgc ttttccagtt ctcgcacggt atgcagaact tcccgccctt
     5281 tctctaataa catttttccg gtgcgggtga atttagcgcg gtgtccgcta cgatcaagca
     5341 attgaatatt taggtcgctt tccagcttgt gaacggtgta actcagagcg gacggcgtct
     5401 tgtagagttt ggctgacgcc gccgcaaaac tgccctcttt ttctatggca tcgaggatca
     5461 tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgcatgcgg cgtcctgctg
     5521 gcgtgatcgt ttactccaac ggcattacca gcggatcggg atactggtat tcaaatccca
     5581 gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc agactatttc
     5641 tgaactgcgg aggttgcagc cccagtaaac gggccatctg cggatagaaa acattacgcg
     5701 cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc tgtaacaaca
     5761 gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt tcaccatcag
     5821 gggcggtttt tccggcaaag aagcgtcccg gatgacgtcc cggtccgacc aggcccgcaa
     5881 gacgcagaat atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg agttcttcta
     5941 atactcgtcc actgttggtt accggattac gcggggtagt ttctttaacc gtgccttgcg
     6001 cgtcgccata gacagatgtt gagctggtaa aaataattcg gggaatacga tgggccagcg
     6061 cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg ccgctacgac
     6121 gtgccggaag cgtaatgacc agcgcatcgg catccataag ggcatccaga tcgtcagaat
     6181 cgcaaactaa ctcaggctcc atgcgaagca gatagctatc aatgccactc attcgggccg
     6241 cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc gctgaaagtg
     6301 acatcgccag cggcatacct aaccacccta acccgacaat tgcgaccttt ttcatccgtt
     6361 atctcctgat acaccttaga tctataaggc tacgctagcg tatcaaaact gacaattcat
     6421 tctatgaatg aatctgttca ataatgataa cgacatgctg caatgagcag tgaatcatca
     6481 gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa
     6541 ttatgtttca ggggttctgg tttcccctta ccttcaaatg gtgttctgcg ggtatctttg
     6601 ataagttcat tgatcttttt aacaattcgc ttatctgttt cctgccagta cagataatca
     6661 tcccatgatt cctcagacca gattagtttc act
//
LOCUS       DAJTRM010000160         6548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRM010000160 DAJTRM010000000
VERSION     DAJTRM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.160"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            7..657
                     /locus_tag="OO322_003860"
     CDS             7..657
                     /locus_tag="OO322_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908541.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO322_003861"
     CDS             629..1063
                     /locus_tag="OO322_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5908542.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO322_003862"
     CDS             complement(1836..2885)
                     /locus_tag="OO322_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5908543.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO322_003863"
     CDS             3336..4526
                     /locus_tag="OO322_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5908544.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO322_003864"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO322_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5908545.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..>6548)
                     /locus_tag="OO322_003865"
     CDS             complement(6373..>6548)
                     /locus_tag="OO322_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5908546.1"
                     /translation="VFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNR
                     PKYIIKKSHRGNP"
BASE COUNT         2051 a         1192 c         1325 g         1980 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaa
//
LOCUS       DAJTRM010000161         6360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRM010000161 DAJTRM010000000
VERSION     DAJTRM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.161"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            240..2672
                     /locus_tag="OO322_003866"
     CDS             240..2672
                     /locus_tag="OO322_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5908547.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO322_003867"
     CDS             2739..4208
                     /locus_tag="OO322_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5908548.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO322_003868"
     CDS             4208..5869
                     /locus_tag="OO322_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5908549.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..>6360
                     /gene="symE"
                     /locus_tag="OO322_003869"
     CDS             6090..>6360
                     /gene="symE"
                     /locus_tag="OO322_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5908550.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCK"
BASE COUNT         1882 a         1502 c         1465 g         1511 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
//
LOCUS       DAJTRM010000162         6244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRM010000162 DAJTRM010000000
VERSION     DAJTRM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.162"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..311
                     /locus_tag="OO322_003870"
     CDS             <1..311
                     /locus_tag="OO322_003870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908551.1"
                     /translation="AMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTH
                     DLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR
                     "
     gene            complement(387..851)
                     /locus_tag="OO322_003871"
     CDS             complement(387..851)
                     /locus_tag="OO322_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5908552.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(848..1723)
                     /gene="nanK"
                     /locus_tag="OO322_003872"
     CDS             complement(848..1723)
                     /gene="nanK"
                     /locus_tag="OO322_003872"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5908553.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1720..2409)
                     /locus_tag="OO322_003873"
     CDS             complement(1720..2409)
                     /locus_tag="OO322_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5908554.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="OO322_003874"
     CDS             complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="OO322_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5908555.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="OO322_003875"
     CDS             complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="OO322_003875"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5908556.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="OO322_003876"
     CDS             complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="OO322_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5908557.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFDEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1583 a         1693 c         1547 g         1421 t
ORIGIN      
        1 ttgccatgat ttacattgaa cagcttgaat taatccataa aagtggtgat gtgttgtatc
       61 cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt ccaccttgtt ccattcggct
      121 taaataagac tcacgacctt ctagaggtgg aagatgcttc agaagcaatt cggctggtga
      181 ttgatgaacg tcattccatt cgctgcagta cacttacagc aactattacg aataaaaagg
      241 gcaagcgaat taagcggaca ggtatataca gtatcaaggg tgttaacata aaagaataca
      301 acgtcaggta atggcacaga gcggtccgtc agttcaggtt tggctctgtg ccaaagatgg
      361 gtcaggtcac acctgatcta ataaatttaa gccattaaat cagccttaac cttcaccacc
      421 acctttttaa tctcacccgg ctcgccaaca acgcatcccg gcttatgcgg ctctcctggc
      481 ataaacacgg cgaacattcc cggtttcaga atgatggttt gctcgttttc aatggcgcta
      541 caaagctggt aatcatcttc atggtggaac tcttcacatt ggcgcgcagt gcctgccatg
      601 ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta ttgctcgtgc
      661 aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaaaata
      721 ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc agctaaggcc
      781 agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca
      841 cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcat
      901 ggcggtaatg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagataca
      961 tttccaccaa cgccagatac ccttctgcca gaccaacgct gccaccaacc accacgcact
     1021 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcgat
     1081 gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg tgtgaaaata gttctcgcat
     1141 ccgcgccagc cagctcgccc tgcgctgccg ccgcaatgcc gcgaccagaa gcaattgctt
     1201 ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca agcgtatgcc
     1261 cgatgtgccc cgccagaccg ccagggccgg taagcagttt gccgccgctc actacaccgc
     1321 cgccgacgcc agtggaaacg gtgataaaga ccatatcggt tatatcgcct tccagcgcct
     1381 gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggtaa
     1441 gttgttccag cgttttgact aacggaaagt gtagcaatcc accgagatta tgcggattca
     1501 gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc cgctgcgcat
     1561 gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct ggtgtctggc
     1621 tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg
     1681 cggcaagttt agtaccgccg atatcaatcg ccagtatggt catagcaccg cctttttcat
     1741 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
     1801 cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc
     1861 ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc agcgccagat ccggctcttc
     1921 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
     1981 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
     2041 atggatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat
     2101 ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca tcttcaatat aggccgtgat
     2161 gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac
     2221 cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga atggcaaccg cgcccgcctg
     2281 ctctgccgct aatgccatgg ctgcgacgat ttcgggttta tcgagcgggc tgtcaggaac
     2341 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
     2401 taacgacata catcttccct tagcgaaagg cccggtacat agaccgggca acaggattaa
     2461 cttttggttt tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt
     2521 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg
     2581 gaaggcatat ccagaccaat cagcaggatc accacaaatg tcaggctgaa cgagagcgat
     2641 gccagcgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt
     2701 gcgccaccca atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta
     2761 tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc
     2821 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
     2881 ataatcagca gctgcgaggc cagcaggcta caaacgtacg ctttgcgggt tcccagccag
     2941 tcaccgagga agccacctac gcagcatccc acggccgcgc caaagccact aaagaacagc
     3001 acattggcga cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc
     3061 gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc
     3121 gttggccagc gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac
     3181 agcccgagaa cagcgacgat tgcagcattt tgcaggttac cggcgaagca gaaccacagc
     3241 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
     3301 atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt tctctttcca gtcttccgct
     3361 tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc gataaagaac
     3421 agcgcacgcc aaccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc
     3481 cccacagaga agcctgaaat caaaaaacca ctggctttgt tacgcagatg ttttggccag
     3541 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc
     3601 agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
     3661 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
     3721 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
     3781 tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg
     3841 aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg ccattgtgca
     3901 cggttgagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
     3961 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
     4021 ccctcacccg gtaggggcga gcgaggggaa aacaactcac ccgcgctctt gcatcaactg
     4081 ctgggccagc gccttcagtt ctggcagata tttttcatct accggtccaa acggtttgcg
     4141 gcacagcggc acagaaacga catccatata atgcaggaca gttttcaggc cgcggaatac
     4201 gcccgttttg atcagtaaat caatgacttt gttgcactca gtttgcagtt tctgcgcggt
     4261 ctggatatcg ccttctttca gcgccttcac gatcccctga tatcgccagc ccatgatgtt
     4321 gtaagtactg ccgataccac catcagcgcc tgccagcaag ccggaagcga agatttcgtc
     4381 gtaaccgtta tagagcacca gatcaggatg ttcacgacgg atctgttcca tctgatagag
     4441 atcgccagaa gtctgtttca gcgcaccgac gccaggcaac gtaaccaggg tgttgatctg
     4501 atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaacc
     4561 atccgctgaa tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata
     4621 gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag
     4681 ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt
     4741 cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc
     4801 ttcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat tgaactgaac
     4861 caggcggcgc agactcgctt tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc
     4921 agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata
     4981 aacgatatac ctttatacct gttataccag atcaattaag caacacccca tacagaaagc
     5041 ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga
     5101 aagcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt
     5161 caggatcatg acggcggatc gcatcaacga tcgcaatatg ctgttgataa ctaacgttgt
     5221 tatgttcgtg cagtgcctga tcggtaaccg ttgggcgtgc ggcaataagc cagtcgagca
     5281 gggcaacgtg gatcgccatg aagattgggt taccggggat ctccgccagc acgcggtgga
     5341 aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg ttgatttcca
     5401 gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg ttcagccgca tagcgcacca
     5461 ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccacca ggatgagaaa
     5521 ggaaatcttt cgccatgccg gaaagctcgc cgatgatagt gtccgcagaa ggtcgagaga
     5581 cacgagcgcg ttcgccgttg tttatttgca ccagaccttt gcgttttaac gctgccagcg
     5641 cttcacgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta
     5701 attgttcacc ttcgtcaaat tcacgacggc ggatcatctg ttccagctct tcttccacca
     5761 tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg
     5821 aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg
     5881 attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcaggtttt
     5941 tctctttgtg cctcatcatt accataatta acggaataat taactattgc gaaaaattaa
     6001 tgtaacgcag ataaacacat cccgattgaa ttattcataa gactattcac aagcattatg
     6061 aatattatga atgtgttctt acaaaataat cataatcgca tattttttaa tgaaaaatca
     6121 cctcacctac aattaaaaac acgacatccg caccataaat agacttgcaa aaaatataac
     6181 atcattgttt tcaatctgcc atttatggga ttgaccgttt tcttttgaca cggagttcaa
     6241 caat
//
LOCUS       DAJTRM010000163         6238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRM010000163 DAJTRM010000000
VERSION     DAJTRM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.163"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            30..1418
                     /gene="narU"
                     /locus_tag="OO322_003877"
     CDS             30..1418
                     /gene="narU"
                     /locus_tag="OO322_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5908558.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1500..5240
                     /gene="narZ"
                     /locus_tag="OO322_003878"
                     /pseudo
     CDS             1500..5240
                     /gene="narZ"
                     /locus_tag="OO322_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5237..6118
                     /gene="narH"
                     /locus_tag="OO322_003879"
                     /pseudo
     CDS             5237..6118
                     /gene="narH"
                     /locus_tag="OO322_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
BASE COUNT         1473 a         1656 c         1660 g         1449 t
ORIGIN      
        1 attaccaatg tgtgcatatg aggaacaata tggcactgca aaatgagaaa aatagtcgtt
       61 atcttttacg cgactggaaa ccagaaaatc cggccttctg ggaaaataaa ggaaaacata
      121 ttgctcgaag aaacctctgg atatcagtca gttgtctact tcttgccttc tgtgtctgga
      181 tgctatttag cgcagttacc gttaatctca ataaaatcgg ttttaatttt actaccgatc
      241 aactcttttt attaaccgca ttaccctccg tttctggcgc attattgcgt gttccctact
      301 cctttatggt gcctatattc ggtggacgcc gatggccggt ttttagtact gcaatcctga
      361 ttattccttg cgtctggctc ggaattgccg tgcaaaatcc gaatactcct tttgggatat
      421 ttatcgttat cgctttgcta tgcggttttg caggtgcaaa ctttgcttcg agcatgggca
      481 atatcagttt cttctttcca aaagccaaac aagggagcgc tcttgggatt aatggcggat
      541 taggaaactt aggtgtaagt gtaatgcagc tggttgcacc gctggtcatt tttgtacctg
      601 tatttgcctt tctcggcgtc aatggcgtac cgcaggccga cggttcggtg atgtcgctgg
      661 cgaatgccgc atggatttgg gtaccgctac tggcgattgc cacgatcgcc gcctggtcag
      721 ggatgaatga tatcgccagt tcacgcgcct caattgccga ccagctccct gtcttacaac
      781 gcctgcatct ctggctgctg agcctgcttt accttgccac cttcggttcg tttatcggtt
      841 tttctgcggg ttttgccatg ctggcaaaaa cccagttccc ggatgtgaat attctgcgcc
      901 tggcgttctt tggcccattt attggtgcca tcgcgcggtc ggttggtggt gctatttccg
      961 ataaattcgg cggcgtgcgg gtgacgttga tcaactttat ttttatggcg attttcagtg
     1021 ccctgctgtt ccttacctta ccgggcacag gctccggtaa tttcatcgcc ttttacgccg
     1081 tatttatggg gctgtttctg accgcgggtc tgggaagtgg ttctactttc cagatgatcg
     1141 ccgtcatctt tcgccagata accatttatc gggtgaagat gaaaggcggt agtgatgagc
     1201 aagctcagag agaagccgtc accgaaacgg cggcaacgct gggctttatc tcagccattg
     1261 gcgcagtggg cggctttttt attccgcagg catttggcat gtcgctcaat atgaccggct
     1321 ctccggtcgg cgcgatgaaa gtgtttttaa tcttctacat cgtttgtgtg ctgctgacct
     1381 ggctggttta tggtcggcgg aagttcagcc aaaaataaga gatcgatgcc tgatgtacaa
     1441 ccatgtcgca tccgacaagg catcagatcc caattcattc ctggagcagg agtcatgtca
     1501 tgagtaaact tttggatcgc tttcgctact tcaaacaaaa gggcgaaacc tttgccgatg
     1561 gtcacggaca ggtgatgcat agcaaccgcg actgggagga cagctatcgc cagcgttggc
     1621 agttcgacaa aatcgtgcgt tccacccacg gcgttaactg tacaggctcc tgtagctgga
     1681 aaatctacgt taaaaatggt ctggtgacct gggaaatcca acagaccgac tacccgcgca
     1741 ctcgccctga cctgcccaat catgaacctc gcggctgccc gcgtggcgca agttactcct
     1801 ggtatcttta cagcgctaac cgcctgaaat acccgctcat tcgtaaacga ctgattgaac
     1861 tgtggcgcga agccctcaaa caacacagcg atccggtact ggcgtgggca tcgattatga
     1921 atgatccgca aaagtgcctg agctacaaac aagtgcgtgg gcgcggcggg tttatccgct
     1981 ccaactggca ggaactaaac cagctgattg ccgccgctaa cgtctggacc atcaaaacct
     2041 acggcccgga tcgcgttgcc ggtttctcgc cgatcccggc gatgtcgatg gtttcttacg
     2101 ccgccggaac gcgttatctg tcgctgcttg gcggcacctg ttaaagtttc tacgactggt
     2161 attgcgacct gccgcccgcc tcgccgatga cctggggcga gcaaaccgac gtaccggaat
     2221 ctgccgactg gtataactcc agctacatca tcgcttgggg gtctaacgta ccgcagacac
     2281 gtacgccgga cgcccacttc tttaccgaag tacgctacaa aggcactaaa accatcgcca
     2341 ttacccctga ctactctgaa gtggccaaat tgtgcgacca gtggctggca ccgaaacaag
     2401 gcactgatag cgccctggcg atggcaatgg ggcatgtgat tttaaaagag tttcatctcg
     2461 ataatcccag cgactacttt atcaactact gccgccgcta cagcgacatg ccgatgctgg
     2521 taatgctgga gcctcgcgac gatggtagct acgttcccgg gcgcatgatc cgcgcatctg
     2581 acctggtgga tggactgggc gaaagcaaca atccgcagtg gaaaaccgta gcagttaata
     2641 ccgcaggtga attggtagtg ccgaacggtt cgattggttt ccgctgggga gaaaaaggca
     2701 aatggaatct ggaatccatt gccgccggta cggaaaccga attgtcgtta accctgctcg
     2761 gtcaacatga cgctgttgca ggcgtggcct tcccctactt tggcggcatt gaaaatccgc
     2821 attttcgcag cgtaaaacac aatccggtgc tggtgcgcca attgcccgtt aaaaacctga
     2881 cgttagtcga tggcaacacc tgtccggtgg tcagcgttta tgatttggta ctggcgaatt
     2941 acggcctcga tcgcgggctg gaagatgaaa acagcgcgaa agattacgct gaaatcaaac
     3001 cgtacacccc agcctggggt gagcaaatta ccggcgtacc gcgccagtat attgaaacca
     3061 tcgcccgcga atttgccgat accgcccata aaacgcatgg gcgctcaatg attatcctcg
     3121 gcgcaggtgt taaccactgg tatcacatgg acatgaacta ccgcgggatg atcaatatgc
     3181 tgatcttctg cggttgtgtc gggcaaagcg gtggcggctg ggcacactat gtcggccagg
     3241 aaaaactgcg cccacaaacc ggctggttgc cactggcctt tgcgctcgac tggaaccgac
     3301 caccgcgcca gatgaacagc acctcgtttt tctacaatca ttccagccaa tggcgctatg
     3361 aaaaagtctc tgctcaggag ttactttcac cgctcgccga tgccagtaag tacagcggtc
     3421 atctgattga tttcaacgtt cgcgccgaac gtatgggctg gctaccttct gcgccgcagc
     3481 tggggcgtaa cccgctcggg attaaagctg aagccgacaa ggccggatta tcccccacag
     3541 aatttaccgc ccaggcgctg aaatcgggcg atttacgtat ggcctgcgaa caaccagata
     3601 gcggcagcaa tcatccgcgt aatttgtttg tctggcgctc taacctgctt gactcctccg
     3661 gcaaaggcca cgagtatata cagaagtatc tgctggggac cgaaagcggg attcagggcg
     3721 aggaactcgg tgccagcgac gggatcaaac cggaagaagt cgagtggcaa actgcggcga
     3781 ttgaaggcaa gctcgacctg ctggtgacgc tcgacttccg catgtccagt acctgcctgt
     3841 tctccgatat cgttctgccc accgccacct ggtatgaaaa agacgatatg aacacctcgg
     3901 atatgcatcc atttattcat ccgctttctg cggcggtcga tccggcctgg gaatcacgca
     3961 gcgactggga aatctacaaa ggtattgcca aagcattttc gcaagtgtgc gtgggccatc
     4021 ttggcaaaga aaccgacgtg gtattacaac cactgctgca tgactctccg gcagagctct
     4081 cacagccgtg tgaagtgctc gactggcgca aaggcgaatg cgatctgatc ccgggtaaaa
     4141 ccgcgccgaa tattgtggcg gtggagcgcg actaccctgc tacgtatgaa cgctttacct
     4201 cgctcgggcc attgatggac aaacttggca atggcggtaa agggatttcg tggaataccc
     4261 aggatgaaat cgatttcctc ggcaaactca attacaccaa gcgtgatggc ccggcacagg
     4321 ggcgtccgct gattgacacc gccattgacg cttcagaagt gattctggca ctggcaccgg
     4381 aaaccaacgg tcatgttgca gtcaaagcgt ggcaggcgct gggcgagatc accgggcgcg
     4441 aacataccca tctggcgctg cacaaagagg acgagaagat tcgctttcgc gatattcagg
     4501 cgcagccgcg taaaattatc tccagcccca catggtccgg tctggaaagc gatcacgtat
     4561 cctataacgc gggatacacc aacgttcatg agttaattcc gtggcgcacg ctgtcgggac
     4621 gccagcagct ctatcaggat catccgtgga tgcgtgcttt tggtgaaagc ctggtggctt
     4681 atcgcccgcc tatcgacacc cgtagcgtca gtgagatgcg ccagataccg ccaaacggct
     4741 tccaggaaaa agcacttaac ttcctgacgc cgcaccagaa atggggcatt cactcaacct
     4801 acagtgaaaa cctgctaatg ctgacgctct ctcgcggtgg accgattgtc tggatcagcg
     4861 aaacagatgc ccgagaactg accattgtcg ataacgactg ggtggaagta tttaacgcca
     4921 acggcgcgct gactgcccgc gcggtggtca gccaacgtgt accgccgggc atgaccatga
     4981 tgtatcacgc ccaggaacgc attatgaata ttcctggttc ggaagtaact ggcatgcgcg
     5041 gcggcattca taactcggtc acccgcgttt gcccgaaacc gacgcatatg attggcggtt
     5101 acgcgcagct ggcctggggc tttaactact acggcaccgt cggttcgaac cgcgacgaat
     5161 tcatcatgat ccgcaagatg aagaacgtta actggctgga tgatgaaggt cgcgatcagg
     5221 tacaggaggc gaaaaaatga aaatacgttc gcaagtcggc atggtgctta acctcgataa
     5281 atgtatcggc tgccatacct gttcggtgac ctgtaacaac gtctggaccg gacgcgaagg
     5341 catggagtac gcatggttta acaacgtcga aaccaaaccg ggcattggtt atccgaaaaa
     5401 ctgggaagat caggaagagt ggcaaggcgg ctgggtccgc gatgtgaatg gcaagatccg
     5461 tccgcgtctg ggcagcaaga tgggcgtaat aaccaaaatc ttcgccaacc cggtggtgcc
     5521 gcagcagatt gatgattact acgaaccttt caccttcgac tacgaacatt tgcatagcac
     5581 accggaaggc aaacatatcc ctactgctcg cccgcgttca ctgattgacg gcaaacggat
     5641 ggacaaagtg atctggggcc caaactggga agaactgctg ggcggcgagt ttgaaaaacg
     5701 tgcccgcgac cgcaacttcg aggccatgca aaaggagatg tacgggcagt ttgaaaacac
     5761 cttcatgatg tacctgccgc gcctgtgcga acactgcctc aatcccagct gcgtggcgac
     5821 ctgcccaagc ggcgctatct acaaacgcga agaagacggc attgtgctga ttgatcagga
     5881 taaatgccgt ggctggcgtt tgtgcattag cggttgtccg tacaaaaaaa tctacttcaa
     5941 ctggaaaagc ggcaagtcag aaaaatgtat cttctgttat ccgcgaattg agtcaggcca
     6001 acccaccgtg tgctcagaaa cctgcgtggg tcgcatccgg tatctgggcg tgctgcttta
     6061 cgacgccgac cgcatcgagg aagcgtcgag caccgagcgc gaagttgacc tctatcgagt
     6121 aggcagcctg acggctgcgg cttgtcatgg tctgcaatta ccgttataaa aacaggcaat
     6181 gtcattgtct ttcaggtggt tatatggccc gttcagctaa accccgtaaa cgtaaacc
//
LOCUS       DAJTRM010000164         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRM010000164 DAJTRM010000000
VERSION     DAJTRM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.164"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO322_003880"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO322_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5908559.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO322_003881"
     CDS             2089..2238
                     /locus_tag="OO322_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908560.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO322_003882"
     CDS             complement(2983..3423)
                     /locus_tag="OO322_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5908561.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO322_003883"
     CDS             3641..4030
                     /locus_tag="OO322_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908562.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO322_003884"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO322_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4588..5421)
                     /locus_tag="OO322_003885"
                     /pseudo
     CDS             complement(4588..5421)
                     /locus_tag="OO322_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5421..>6200)
                     /locus_tag="OO322_003886"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO322_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5908563.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1433 a         1617 c         1641 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagccacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatatagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTRM010000165         6126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTRM010000165 DAJTRM010000000
VERSION     DAJTRM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.165"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO322_003887"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO322_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5908564.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO322_003888"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO322_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5908565.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO322_003889"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO322_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO322_003890"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO322_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1613 a         1441 c         1449 g         1623 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaac
//
LOCUS       DAJTRM010000166         6098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRM010000166 DAJTRM010000000
VERSION     DAJTRM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.166"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            612..1790
                     /gene="ltrA"
                     /locus_tag="OO322_003891"
     CDS             612..1790
                     /gene="ltrA"
                     /locus_tag="OO322_003891"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5908566.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1873..2052
                     /locus_tag="OO322_003892"
                     /pseudo
     CDS             1873..2052
                     /locus_tag="OO322_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2082..2564)
                     /locus_tag="OO322_003893"
                     /pseudo
     CDS             complement(2082..2564)
                     /locus_tag="OO322_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2820..3800)
                     /locus_tag="OO322_003894"
     CDS             complement(2820..3800)
                     /locus_tag="OO322_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5908567.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3800..4999)
                     /locus_tag="OO322_003895"
     CDS             complement(3800..4999)
                     /locus_tag="OO322_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5908568.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5321..5659)
                     /locus_tag="OO322_003896"
                     /pseudo
     CDS             complement(5321..5659)
                     /locus_tag="OO322_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5726..>6098)
                     /gene="istB"
                     /locus_tag="OO322_003897"
     CDS             complement(5726..>6098)
                     /gene="istB"
                     /locus_tag="OO322_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5908569.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1657 a         1401 c         1452 g         1588 t
ORIGIN      
        1 ccggtccagg agccgggcag cggtaaaaca aggactgccc ggctgtgggt ctacgttcgt
       61 gatgaccgta acgccggttc ggaaattgca gcataagtta tccatgctct gaggtgggta
      121 gaggtgggcc ctccggtcag ggtttacagg agcacagacc aaacgacccg gtgctgcttc
      181 attctaagga atgaggtggt gagcgactcg ggaaaaggca gagatctact gtcacgtggg
      241 ttcgggaagc tgaacgaaag taaactgtcc gatgaagcat cgaaatctta gatgatgcca
      301 aaatggggcc gtacagccgt cccctgataa gcgcagaaga cacctgctta ctgactgcgc
      361 ggcatccggt gtgaaggcag caggatcccg ggccagacca tctcagggaa cgtgagaacc
      421 tgtttccggc acgtatcggg aaacaggggc ggacccattc gtagtagtga tgaaagatct
      481 gtaatgggtc tggagctaag ggatggggat cacattcatt gcagaagaca caacccaaaa
      541 ggggatgacg taactgatgc aacgaaaatc atttgaaata ccaaaagcac tggtctgggc
      601 atcatatctg gatgtgcgtc gcaacaaggg agccccccgg atgcgacggg cagactttga
      661 aaatgttcga ccagcaacgt gatggcaatc tatacaagat ctggaaccgg ctatgttcgg
      721 gaacatggtt tccgccacca gtgctggaaa aacggatccc gaagtcgaat ggcaaggaac
      781 gcattctggg gatcccaaca gtatccgatc gaattgctca gggagcgata aaacttttca
      841 tggaagaaaa gcttgacccg attttccatg ctgattcata tggttaccga ccaggcaaat
      901 cagctcatga cgctctgaaa caatgtgcca tccggtgctg gcgttatagc tggatactgg
      961 aagtggacat cagtgcgttt ttcgaccatg tgagacatga tctggtgctc aaagctctgg
     1021 aacaccacgg gatgcctaaa tgggtcatcc tgtactgtcg acgctggatg gaagcgccaa
     1081 tgcagagttg tgaaaatgga gaattaataa cccgaacacg aggcactccg cagggcgggg
     1141 tcattagtcc gctacttgct aacttgttcc ttcactatgc atttgatttg tggatggaaa
     1201 gagaatatcg gggggtaccg tttgagaggt acgctgacga tattgtagta cattgttcac
     1261 gaatgagtga tgcaacaagg ctaaagaaca ggttatcgga gcgcttctcg gaagtcgggc
     1321 tggtcctgaa tgcagggaaa acgaatatcg cctacattga cacgtttaaa aggagaaacg
     1381 tcgcaacgag tttcaccttt ctcggatatg acttcaaagt gcgtacgctg aagaatttca
     1441 acggcgaacg gtaccgaaaa tgcatgccgg gtgcgtcaaa tgcagcaatg cgcaaaataa
     1501 cagaaacaat caagaagtgg cgtatacatc gctcaacagc tgagagtttg ctggattttg
     1561 cgaggcgcta caatgcgata gtgagaggct ggatcgagta ctacggaaag ttctggtcca
     1621 gaaatttcaa ctatcgactg tggagtgcaa tgcagtcacg tctgctcaag tggatgcagt
     1681 ctaaatacag actttcgaac cggaaggctc agcgaaagct gacgctggta aggaaggagt
     1741 atccgaagct atttgttcac tggtatttac tacgtgcatc aaatgagtga tcaagagccg
     1801 tatgacgcga gagtgtcacg tacggttccg tgagcagccg gggagtgaaa ttctctccgg
     1861 ctgactcgac tcgcgggttc cgacagcgat gacgaacatg cggcggtgtt gtactcgctg
     1921 atcggcacat gccgtctgaa caatgtggaa ccagaaaaat ggctgcgtta cgtcattgag
     1981 catatccagg actggccggc aaaccgggta cgtgatctgt tgccctggaa agttgatctg
     2041 agctctcagt aaatatcaat acggttctga cgagccgctt acttttggcg taattctgct
     2101 gcgcctttac tgaacacaat gccgtattca gcaagaagtc cacgaatgtg atttatcagt
     2161 tgtgtccttt ccattgaaac caaccgacgc atattcaaaa aagtctggac gtcctgttgc
     2221 tccagagttt ttattggtac gggtcgtata gttccatgtt ggcaggcctc agctattgcc
     2281 tgtgcgtcgt tataatcatt tttctggcac cgctgataag ctttaacatg ttgtgccgga
     2341 agcaatatta catcatggtc gagtttcctg atttcccggg cccagtaatg agatgccccg
     2401 ccagcctcca tcgcaacagt cattttaggc tgttgcacca gccagttaag cagttgattg
     2461 cgagaaagtt tggtctttcc tgcgggtttg ccatgagcat tgatactgaa aagataaaag
     2521 ttcgttttag ccagatcaat gccggtgatt ttctgttcag acatggtatc ccgcttactt
     2581 ttgttgttaa agtttatcca tcatggcgca attgaaggct gccgtgaaag agggggaaga
     2641 ctatctcatc ggttactgtt aaacggctaa gagaaactat ctttttcaag caaagaagat
     2701 acggtgaaac aggcgatttc accgtgaaat actgttcaaa aaaaacgcta ataaaagcaa
     2761 aatttctttt aaaacagcca attaaaaact ggcgagttgc tggcgaaaaa cttgaatttt
     2821 cacagcgaat tcagattatc ttttagtaca agagcaatca tacgatctaa cttatcctga
     2881 acttccaacg gtaatctgcc gaattcatat gagaacgtac gtccctttac tcgttttcga
     2941 gcaaatttat ctttactatc aaatttccat aacaaagtta caaccgcttt atcctttact
     3001 cctgaatcaa gcaatgaaga catttccttg gttataagcc gcataatact gtttttaacc
     3061 tcgtcttcag aaagattgtc atcagcagta ataagctcaa tactcggcga gatattcttc
     3121 acaacttcat caatagatat atcacccagc ctcagtcgtt cacttaattc agccagttgc
     3181 ctgtaatcgg cgtatgtcag ctcagactga acaggaaaaa gagacacaaa atctttaggt
     3241 acggacgctg cttggagggc acgagtcacc ttagctgccg acaatccctc tctttccgca
     3301 atttgcttct ggttcatacc ggcctctttg agacgtacta aacgcaaccc aatttcacga
     3361 atattatgtt caagtgacgt ctgcaaatct ttcgccagat gctgagcttc agaaacagta
     3421 agctcatcat ccgttacaag tacacgtaat ccaaccttgc atagcaaagc tgctgcccga
     3481 cgacgggaac catccaaaat ctcaataaga tcgccagtcc gaatcccaat acatggatag
     3541 aattgttgta aacgtatagt tctggtgatg tcttttagtg agtccggcgt cagggctgtc
     3601 tgttcgcggc cattcacctc ttcaaccaca aaagtttgag tatccacttt gtcatgatca
     3661 accttgatct cagtaaattt tgcctttcgg ccagtattga gggtaaatac atgaactgga
     3721 gccgatattt cttcagtatt attcaggata tttgtattca gcgtacggcc aatagtcggt
     3781 cggtgctttc tgttttccat cagtagttgg cccttataaa ttcaatacga tcaaaaactg
     3841 ctttagcgaa atcttctgct gccatacgtg catttttcaa agcttcacca ctgccaacat
     3901 aagtaacagg attcgcagat atcacggtat caaacgactc gccgcttctc tcaaaaccat
     3961 caagacgtgg cattgaaaca tcaagcatgt caccaccgaa aatctctttt gtcaggctat
     4021 ggcagtattt atgatctgac ttattcgcca gtttcgacat gaagccaata tttgcctgca
     4081 aacggcatga acatccatcc tgttcaataa tctgtacaag ttcaggtaaa cgagccaaat
     4141 atttaagcgt ggaatggaaa tcaacttgtg ctggcggcac cggtgtaaac atgatatctg
     4201 cggcagcaat ggcatttttc aaaaatgcgt ccagatgcgg gcctgtatca ataaggatga
     4261 agtcgaaatc atgcttcagc ttatcaataa tattctcacg caatatcgcg tgtttgtttt
     4321 gtcctaatag atgctcctcg cacagagtat cccagtttga tgcaataaag gcgtcgtcaa
     4381 tagaagccgg cataacataa acgccaggga taactgacgg aacgataaaa tcttccagta
     4441 attcctcacg actcacgttc tgcaacatag cctgcggtgc ggttgtatca accagcccca
     4501 ccgcatgcaa gtagttcaga aacatagtgg ctgatgactg gggatctaaa tcaaggagca
     4561 gaatacgtaa atcttctgac agcaggtgag gatgagcacg cagggcgtga gctacggaaa
     4621 cactactcac ggttttagac acaccgcctt ttagactacc gatgaatatg gaatatgcct
     4681 cactataacg atcgcgatat ttaggaatgc cacgatgtgc atagatatcg ataatgttct
     4741 ggatggttaa tgcatatttt gtcgcggttc ctgctgggcg tttttcaaac tgatagccct
     4801 gagcctccat ctcaccgact gcaccatcca cgcttgcgcg cgttaatttt ggaagcttag
     4861 ctacagcaga ctttgaatag acttgataaa aaacatcagc ctgaaactcc tgcttttgct
     4921 cctgaatctg ttttgttagg gcgagcaaca ttttatctgc acgctgcgca accttactta
     4981 actgctcaaa actagtcatt ccccctccgt gctatgtagg attttaactt attacaaaaa
     5041 aaactgcatt agcagggtaa tgcatgcaag tacatattgt acatgcttat attaaaaatg
     5101 cgcgcattgc tgatttatta atcatcggaa tgaatattac tgtaagatag tatgcctttc
     5161 cgggagaaag acgctgattt cccccgaata atccagttca ccaccacact ccggacaaca
     5221 gctttcttca ggctccagat ggtgtatttc gcgggaaaca tgttccggta acggacggcg
     5281 atgacgtgac tgtcgcaaca ggcgggggta gtgcttccaa tcagtagaac atgtgctttt
     5341 caatgaagga gccgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt
     5401 tacgtgccag ccgcttgatg cgggtgcgga gggtccggtt gttacgtgca atacgttgcg
     5461 tgaagatttt gccggtcagg tgtttctcct tcggcacttc tctggcgtag ctgccccagt
     5521 catcgctggt caccatgccg atacagaacg gagtcaacag ggctaacagt tcccgacagg
     5581 tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta gtgttgtacg
     5641 cataccagag ccagtgttgt gtcaacgacg gatgaaaagt gatccactta tatctccacc
     5701 aacggcccaa tattgatcca ccgttttact caggattagc ttctgctata accccggcct
     5761 ttcgtttctg tctgagtcga tagctttctc ctttgatttg aacgacatgt gagtggtgta
     5821 agatacggcc cagcatcgct gaggtcagtg ctgcatcacc ggcgaacgtt tgatcccact
     5881 gcccgaacgg tagattggat gtcaggatca ttgcgctctt ttcgtaacgt ttagcgatga
     5941 cctggaagaa cagttttgct tcttcctgac tgaacggcag atagcctatt tcatcaatga
     6001 tgagcaggcg gggggccatt actccacgct gaagcgtcgt tttataacgg ccctgacgtt
     6061 gtgccgtaga taactgaagt aacagatctg ctgctgtt
//
LOCUS       DAJTRM010000167         6097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRM010000167 DAJTRM010000000
VERSION     DAJTRM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.167"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..393)
                     /locus_tag="OO322_003898"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO322_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO322_003899"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO322_003899"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5908570.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO322_003900"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO322_003900"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5908571.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..6097
                     /locus_tag="OO322_003901"
                     /pseudo
     CDS             5797..6097
                     /locus_tag="OO322_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1868 a         1387 c         1048 g         1794 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
     6001 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
     6061 tccttcgcct ggcttccgct tattttgcaa aggcgga
//
LOCUS       DAJTRM010000168         6034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRM010000168 DAJTRM010000000
VERSION     DAJTRM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.168"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            147..1499
                     /gene="celF"
                     /locus_tag="OO322_003902"
                     /gene_synonym="chbF"
     CDS             147..1499
                     /gene="celF"
                     /locus_tag="OO322_003902"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5908572.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1512..2270
                     /gene="chbG"
                     /locus_tag="OO322_003903"
     CDS             1512..2270
                     /gene="chbG"
                     /locus_tag="OO322_003903"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5908573.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2317..4578)
                     /gene="katE"
                     /locus_tag="OO322_003904"
     CDS             complement(2317..4578)
                     /gene="katE"
                     /locus_tag="OO322_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5908574.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4761..5024
                     /gene="cedA"
                     /locus_tag="OO322_003905"
     CDS             4761..5024
                     /gene="cedA"
                     /locus_tag="OO322_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5908575.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5301..6034
                     /locus_tag="OO322_003906"
                     /pseudo
     CDS             5301..6034
                     /locus_tag="OO322_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1539 a         1289 c         1533 g         1673 t
ORIGIN      
        1 cctaagagat atcgtaagaa attgactgaa tttaatcagt aagtcgttat acctgacaat
       61 tcacatatct gtcctgttgc tggattattt atgtccgggg gggcagatat gccagatatc
      121 agtattctgt actgaaggga gaaattatga gccagaaatt aaaagtcgtc actattggtg
      181 gcgggagcag ctataccccg gagttactgg aaggatttat taagcgttat cacgaattgc
      241 cggtcagcga attatggctg gtggatgtcg agggtggcaa agctaaactg gatattattt
      301 tcgatctctg ccaacggatg attgataacg ctggcgtccc gatgaagctt tataaaacgc
      361 tggattgccg cgaagcattg aaagatgctg atttcgttac tacccaactg cgcgttggcc
      421 aattaccggc gcgtgaactg gatgaacgta ttccattaag tcatggttat cttggtcagg
      481 aaaccaacgg cgcgggcggt ttgtttaaag gtctgcgtac cattccggtg atttttgaca
      541 tcgtaaaaga tgtcgaagaa ctttgtccga atgcatgggt gattaacttc actaacccgg
      601 cgggaatggt tactgaagcc gtttatcgtc ataccggatt taaacgcttt atcggcgtgt
      661 gtaatattcc gatcggcatg aagatgttta ttcacgatgt tctgatgctg aaagacagcg
      721 atgatttatc tatcgatttg tttggcctca accatatggt gttcattaag gatgtgctgg
      781 taaatggcaa gtcgcgcttt gccgaattgc ttgatggtgt ggcatccggg cagttaaaag
      841 tatctggcgt taaaaatatt ttcgatctgc catttagcga aggcttaatt cgttctctga
      901 atctgttgcc gtgttcttat ttgctttatt acttcaagca aaaagagatg ctggctattg
      961 aaatgggcga atactacaaa ggcggcgcac gagcgcaggt agtacagaaa gtcgagaaac
     1021 aactttttga gctgtataaa aacccggagt tgaaagttaa gccgaaagaa ctggaacagc
     1081 gcggtggggc ttattactct gatgcagcgt gcgaagtgat caacgctatc tacaacgaca
     1141 agcaagctga acattacgtt aatatcccgc atcatgggca tattgataat attccggcag
     1201 actgggcggt agaaatgacc tgtaagctgg ggcgcgatgg cgcgacgcca catccgcgca
     1261 ttacgcattt cgatgataaa gtgatggggc tgattcacac cattaaaggc ttcgagattg
     1321 ctgccagcaa cgccgcactt agcggagaat ttaacgatgt gttactggcg ctaaacctta
     1381 gtccgttggt gcattccgat cgcgatgctg agctgctggc acgcgagatg attctggcgc
     1441 acgagaaatg gctgccaaat tttgccgact gcatcgcaga gcttaaaaaa gcacattaac
     1501 cgaggctgat tatggaacgc ttactgattg ttaatgccga tgattttggc ttaagcaaag
     1561 gtcagaacta cggcattatc gaggcctgtc gcaatgggat tgtcacatcg acgacggcgc
     1621 tggtgaatgg gcaggctatt gaccatgcgg tgcagttgag tcgtgatgaa ccgagtctgg
     1681 ccatagggat gcactttgtc cttactatgg gcaagccact gacagctatg ccggggttaa
     1741 cccgcgatgg tgtgctggga aaatggatct ggcagttggc agaagaagat gctttaccgc
     1801 tggaagaaat tactcaggag cttgccagtc agtatttgcg tttcattgag ctatttggac
     1861 gcaaacctac gcatcttgat agccatcatc atgtgcatat gttcccgcag attttcccga
     1921 ttgttgccag gtttgcggct gaagagggga ttgcgttgcg catcgaccgt cagccgctat
     1981 ctaacgctgg tgatttaccg gctaatctgc gcagttcgca gggattcagt agcgcgtttt
     2041 atggtgaaga gattagcgag gcactgttcc tgcaagtgct ggatgacgca agccatcggg
     2101 gtgatccttc gctggaggtg atgtgtcatc cggcgtttat cgataatact atccgtcaga
     2161 gtgcttactg tttcccacgg ttaacggagc tggaagtgct gacatcagcg tcgttgaaat
     2221 atgcaattgc tgagcgtggt tatcggttgg gtagttatct tgatgtgtaa gtaaggtggt
     2281 aaggtcactc cccaggggac gtgaccttat ccatatttac gccggaattt tgtcaatctt
     2341 aggaatgcgt gaccacacgc ggtgttctgc catcagcgtt aacagttcat ccataaaact
     2401 accgtcggtg ctgtcagctt ccacaatccc ttcttcaccc tggtcagcta ccttgattgt
     2461 tgctttaaac ttgcgcgcgt ctcccgccag cgcaatcggt ttaaggtgtt tgtaggcttc
     2521 catcagatag tagttggcat cgccgttgtc agcgatatcc gcgatattgc cgcaagggac
     2581 aatgaccgca tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta taggcaacac
     2641 cgtaccgtca tccgcagtca cttcacccat tcgggagtag agcagtttgg catgaacgcc
     2701 tttggccttc atggccttga gaatggccag aaggtctgcc gatctcactt catcattaag
     2761 taaaatcgct accacgcgac ctttcacatc accgtcagga atggcgtaca aacttaagga
     2821 tggatccttt ttcagaccgt tgacgtccgg aggtggggtg atattcagct ggtcgtcagt
     2881 cagttcgata ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa tatgcgccag
     2941 ctggtcaaca acgcgctcac gaatatacgg acgaacgact ttgcttaact caaaactgaa
     3001 accatcgaca atatggcgct gctcgaatgg cgtctgactt agccagaaca gacgcggatg
     3061 ggaataatat tcgccaaacg atgggctgcg ctcgcgaact ttattgcctt ccacgcgctc
     3121 ttggtatgat tcaaaaccgc cgcgtttcgg ccccggtggt gtttcgcgcg gccagttatc
     3181 gttgatcgag ttcggttcat aattcgccgg gttagtgtcg atccccatac gatgcatgcc
     3241 gtcacgctgg aaattatggt aagggcaggt cgggcggtta atcggaatct catggaaatt
     3301 cggcccacca agacgactga tttgtgtatc ggtataggag aacaaacgtc cctgcaacag
     3361 gggatcgttg gtgaagtcca gaccaggaac aatatgacct ggatggaatg ctgcttgttc
     3421 gttttcggca aagaagttat ccggattgcg attgagcacc attttgccga cacgctgaac
     3481 gggcaccagt tcttccggga tcagtttggt tggatcgaga agatcgaagt cgaacttgaa
     3541 ttcatcttct tcaggaatca actggaagcc cagttcgtat tccggaaaat cgcctgcttc
     3601 aatggcttcc cacaactcgc ggcggtggaa gtccgggtca cgtccggtga gtttttgtgc
     3661 ttcatcccaa acgagtgagg ctttacctgc cagtggtttc cagtggaaac gtacaaacgt
     3721 tgccttccct tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt ccatggtgcg
     3781 gtaactgcgg gggatgccgc gatccgacat cgcccacatc acgttgtgca gagtttcagg
     3841 ttgcagagaa acataatccc agaaagtatc gtgggcactt tgcccttgtg gaattgccca
     3901 gtgcggttct ggttttaccg catgaacaaa atcggggaat ttatgcgcat cctggataaa
     3961 gaagattggc gtgttattgc caacgaggtc aaaaataccc tcttcagtat agaacttggt
     4021 ggcaaaacca cggatatcac gcacggtatc ggcagagcca gcgccaccct gaacggtaga
     4081 gaacctaaca aatactgggg tgattttgtt cggatctgag aggaaatccg ctttggtgat
     4141 atcgcttaag cttttatatg gctggaaata accgtgagct gctgagccgc gtgcatgaac
     4201 aatacgttcc gggatgcgct catggtcaaa gtgggtgatt ttctcgcgca gaataaaatc
     4261 ttccagcagc gttggaccac ggttaccggc acgcagtgag ttttgatcat cggcgatgcg
     4321 cacgccctga ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt cttccagaga
     4381 attaagtttt tcgttacgtg tatcaggggc tttcaggctc ccaggggcgg taggttgtgc
     4441 acctggcggt gttggttcag ccgctggacg atgagatccg tcctcaggtg ccagtgagtc
     4501 catccccggt ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat gtgggttctt
     4561 ttcgttatgt tgcgacattg aactcgtctc cttaatttat tactgaaagg gccgctgttt
     4621 tggtcaaaaa actctctaac tatagaccac cagccagatc ccgcttcgga gactattctg
     4681 gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag gggctaaatc ggctaaacta
     4741 cagtttttct aatttttaat atgcgtttag tgaagccagt aatgaagaaa ccgctccgcc
     4801 agcaaaaccg ccagattatt agctatgtcc cacgcacgga acccgcgccg ccagaacatg
     4861 cgataaagat ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat gttgcgtttg
     4921 tactgatggg agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa tctgcccaac
     4981 gttgggcaaa tcagatccgc caggaagggg aagtgactga gtaaggatta catacctccc
     5041 ggttttgccg ggagttacag gacctgaaat tctgcatttc gttgctacat tcattcaaac
     5101 cacctgtaac aaaaagctgc ctttgacgag ctattgctat aaataagaga tcttttttct
     5161 ttaattattt atttatttta aaatggttaa gttttgctaa tcatgatgag ttatttttga
     5221 tagttcatcc tgtggcgtcg ctcttaaact tatattgtaa atgacttaga tttaaataag
     5281 ataataattt tgttaacaat atgaggcggg agatgttttc tacaaactta atacagagta
     5341 attatggtga tttaaatatt aaaagcctgg ctttcgactc ttttaaagaa aggcttgagt
     5401 ctaccatggc ggcactcact ttctttatat caacgggtca atgtgactgc gatgaaatag
     5461 cagagtcgaa cttcaatcgt atgattgcct atatgtctaa cattaactat gatgcctcaa
     5521 agctaggagc gccagcactt agttttgata cgtatctgca ggacaatgtt aaatatagag
     5581 tgataattaa taatctttat ggcagtgagt ttcggataag agatattaat gagaattttg
     5641 ttgggatgga ttttacctgt gaattccgac ccgaaaaaat gacgaatttg atcaatataa
     5701 aaaataggtt ggttattcat tatttaagga ctatatacta tgaaaaaaat tatatacatc
     5761 ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa gtttccttcg
     5821 ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga
     5881 agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg
     5941 tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg tattgataag
     6001 ttggacattt ttttcgctga cgatctgttt aaat
//
LOCUS       DAJTRM010000169         5926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRM010000169 DAJTRM010000000
VERSION     DAJTRM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.171"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..133)
                     /locus_tag="OO322_003907"
     CDS             complement(<1..133)
                     /locus_tag="OO322_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5908576.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     "
     gene            369..641
                     /locus_tag="OO322_003908"
     CDS             369..641
                     /locus_tag="OO322_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5908577.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(607..1014)
                     /gene="nudG"
                     /locus_tag="OO322_003909"
     CDS             complement(607..1014)
                     /gene="nudG"
                     /locus_tag="OO322_003909"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5908578.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1101..1730
                     /locus_tag="OO322_003910"
     CDS             1101..1730
                     /locus_tag="OO322_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5908579.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1739..3046)
                     /gene="ynjE"
                     /locus_tag="OO322_003911"
                     /pseudo
     CDS             complement(1739..3046)
                     /gene="ynjE"
                     /locus_tag="OO322_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3113..3766)
                     /locus_tag="OO322_003912"
     CDS             complement(3113..3766)
                     /locus_tag="OO322_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5908580.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3766..5237)
                     /locus_tag="OO322_003913"
                     /pseudo
     CDS             complement(3766..5237)
                     /locus_tag="OO322_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5210..5926)
                     /locus_tag="OO322_003914"
                     /pseudo
     CDS             complement(5210..5926)
                     /locus_tag="OO322_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1401 a         1577 c         1566 g         1382 t
ORIGIN      
        1 attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga acggcttgcg
       61 cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac tccagagaat
      121 atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt atgttgttta
      181 tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta agcaagaaaa
      241 aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat gcgataactc
      301 agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat aacgttaagg
      361 agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta attgcactgg
      421 ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg ttcagttcag
      481 gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa aattattctg
      541 aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat gacaaaaccc
      601 ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta ataatggaat
      661 gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga ccagcgcctg
      721 atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat gaagatggat
      781 aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag ttgcttcgat
      841 gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat ccagctcgac
      901 tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc gttgcgcgag
      961 taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt tcataaatta
     1021 cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa tcagaaaggc
     1081 tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt aaaccgttgt
     1141 tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt ttaacattag
     1201 tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg tatctggcgg
     1261 cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg ctggcgcggc
     1321 gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt ctcttttacg
     1381 cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg gcaggcggct
     1441 ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct gcactggcag
     1501 caaaacatca gattgataac cccggttatg cacataagtc gttttactat ctggggggat
     1561 taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc cctgcatggt
     1621 ttgcgtggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
     1681 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
     1741 atttgctact gcccgggccg cgttcaccgg ttgcaaccgg attttttgga tcgctgctcc
     1801 attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca
     1861 taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgatctggtt
     1921 tgatattcca tgctttccac atagcggtaa tatcatcggc gctacgcatg gtgccatccg
     1981 ggttatagaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg
     2041 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
     2101 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca
     2161 tatcgagcat caactgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca
     2221 ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc
     2281 catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc
     2341 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg
     2401 ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
     2461 cgttggtatc gatgtagtca gcgccaggaa tatggctgat aaggtaaagc ttaggagcgc
     2521 cctaggccgc ttcaatgact ttccagtcac cgacaggttt cgccgtaacc tctttacctt
     2581 gttgcaggtc atgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta
     2641 gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt
     2701 gcagtcgcgt tttgacggcg tcaacatctt tgccattacc gtacagcgcc accggggcat
     2761 cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcca
     2821 gccagctagc agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt
     2881 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgct
     2941 gaagttggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
     3001 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
     3061 ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca
     3121 gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat
     3181 cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ggcgcgaact tcgctgaaca
     3241 cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga
     3301 gtaacgcttt tggctgggcg agaagggcgc gtagtagagc aacgcgcgct cgctgaccgc
     3361 cagacaaagt ggcaggatcc tgatggaaag ctccgtcaag gcctgaacgc tcaagtgcat
     3421 cattcacggc attacgtcgg gcattcccct taagtgtcgc cggtagcgcc agcagtaaat
     3481 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg aaaaagaatg ccaatctgac
     3541 gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag
     3601 aaaactgtcc ggccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag
     3661 acggccccat taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca
     3721 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggattccttg
     3781 tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa
     3841 caacagttgc cataacgcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
     3901 caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
     3961 ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcacagggc gcaacatgag
     4021 tgggcatttc acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
     4081 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
     4141 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatccaggt tcagccatag
     4201 cgccagcgtg tactggcccg ccaccagtgg cagagcgggc aatagaatgg gcaaccatag
     4261 ccacaactgg cgacgctgtg ccccccattc cagccacagc agtaacagga gcaaagcgat
     4321 gaatgttgct accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga
     4381 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
     4441 cgccagcgta gtgcctggta ataaaggtgt ggcaggcttg cgaatgccat ctacgcgggg
     4501 aatagtgcgc cgccagctgc gccaaaacgt aggcggcgag taacagcatc agcagcagac
     4561 tggcgagtgc gcctttcgtt tgttgatccg cgtcgccctg agttaaccac tgccagctaa
     4621 ttactgccag cgtcggtgga ttacctggcc caagaataat cgccacatcc acgaccgaaa
     4681 gcgaccaggc aacaatcgcc agcatcgcca ttgccagcgg ctgtagccca gcgaatccag
     4741 cacaatgacc tgctgcaaca gccgtttttc gctcaatact gccactaaga tccacagcag
     4801 aaatgcgctt tctttcactg caagggtgag gcccagaccg atccaaatcg gtccattggc
     4861 ggagtgaaat acgggaaata gtcatatagc agccctccgt cagcaaagag cagcagggcg
     4921 ctggtggcaa aagccacatg gggaatggcg agcagccacg gcagacgggc gcacatacgc
     4981 tgccatttcg gcccaggcca tagcgccaca atcaccagca gggcaatcaa caatgccccg
     5041 accgccgcga tggttgtcga caccagcgtt gccagtaatg cctgcggtaa ctgtggatcg
     5101 gcaaataacg cctgccagcg tgtcaacgac aacgcaggcg atatcagcgt gagagctgcc
     5161 gggatcagcg gtgcataaat caccgccacc atcgcccaca gcaaaaagat taatgcgtac
     5221 cgtaacggcg taaccattct tgttccagtg cattcaccca tcctgcgtgc ggttcagcca
     5281 gtaccggcgg cagatcctgc ggcattcttg attgcaatat ttcgcgctgc ccgtcaggca
     5341 gtttttgcgg atcgagaaca gaaggatcgc cccagacaac gggatctgct ttacgcagtt
     5401 gcgcatcggg tgagagcagg aaattggcaa ctaccttcgc cgcagcactg gcattcgcgt
     5461 tggctggaat ggtgacgaaa tgcacgttgc caatcatccc ctcgcgaaag ccaaaactgt
     5521 aactgcttgc cggcaaatcg ccgctggcga ttttttgctg cgcatgtgcg gggttaaagg
     5581 tcagcgacag gcgcaatgtg ccggctttca gcagagcatc catccgcgcg ggcgaaggcg
     5641 ggaaatcctt tccttcgcgc cacaaatacg gatgcagtgc atcaagatat tgctacaagg
     5701 gcgcagtgac acgggcgaaa gtcgcatcgt ccggcgcttc ttttaatgcg gcgggatctg
     5761 gcgtcagcat aatcagcaac tgttcaagaa acgccgtgcc ggtaaagtcc ggtgggcgcg
     5821 gataagtaac cgtgccggga ttggctttag caaactccag taaggcttgc ggcgtttgtg
     5881 gtggctgtgg cgtaacatcg cggcgggcga taaacgtcag ttgtgc
//
LOCUS       DAJTRM010000170         5869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRM010000170 DAJTRM010000000
VERSION     DAJTRM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.172"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..630
                     /locus_tag="OO322_003915"
     CDS             <1..630
                     /locus_tag="OO322_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5908581.1"
                     /translation="NVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFL
                     NVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSRKSVLFW
                     ILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVM
                     FIAIDMDKLKQINDTLGHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            673..1176
                     /gene="yeaK"
                     /locus_tag="OO322_003916"
     CDS             673..1176
                     /gene="yeaK"
                     /locus_tag="OO322_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5908582.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1177..1281)
                     /locus_tag="OO322_003917"
     CDS             complement(1177..1281)
                     /locus_tag="OO322_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908583.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1451..1897
                     /locus_tag="OO322_003918"
     CDS             1451..1897
                     /locus_tag="OO322_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5908584.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1854..2672)
                     /gene="nimR"
                     /locus_tag="OO322_003919"
                     /pseudo
     CDS             complement(1854..2672)
                     /gene="nimR"
                     /locus_tag="OO322_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2769..3949
                     /gene="nimT"
                     /locus_tag="OO322_003920"
                     /pseudo
     CDS             2769..3949
                     /gene="nimT"
                     /locus_tag="OO322_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4004..4351
                     /locus_tag="OO322_003921"
     CDS             4004..4351
                     /locus_tag="OO322_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5908585.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4373..4627)
                     /gene="yoaF"
                     /locus_tag="OO322_003922"
     CDS             complement(4373..4627)
                     /gene="yoaF"
                     /locus_tag="OO322_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5908586.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4810..5835
                     /gene="dgcP"
                     /locus_tag="OO322_003923"
     CDS             4810..5835
                     /gene="dgcP"
                     /locus_tag="OO322_003923"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5908587.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
BASE COUNT         1405 a         1439 c         1442 g         1583 t
ORIGIN      
        1 aacgtgatga gtttgctgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg
       61 ctggatatta acaaaaacaa tttgcggaat atcttttaca ctcatgaccg ccctctcctc
      121 tggcgttttc tcaatgtcac gctaaccgat accgattcgg ggcgcgacat tatcatcaac
      181 cagagcgaag ataatctgtt ccagtatgtc agttacgtcc atgacttacc tggcggcatt
      241 cgtgtctcgt tatccattga tattctttac tttatcacgt cttcgaggaa aagcgttctg
      301 ttctggattt tgacggcgtt aattttgctg aatatggtcc ggatgcactt ccgtttatac
      361 caaaatgtgt cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt
      421 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
      481 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
      541 ggggatttag cgattacgtt acatgatgcg tataaagctt ccgatgagcg tttatatgtc
      601 aataagcaga acaaaaacag ccgttcatga taaccttctg tggttgtttg cttgtaatct
      661 caggagcgtg aaatgactga aatggctaaa ggaagcgtga cgcatcagca gttaatcgct
      721 ttattatcac aagaaggtgc taacttccgc gtcgttaccc atgaagcggt aggtaaatgt
      781 gaagcggtgt cagaaattcg tggtaccgct ctaggccagg gtgctaaggc actggtatgt
      841 aaagtcaaag gcaatggcgt aaatcaacat gttctggcga tcctcgcagc cgatcaacag
      901 gccgatctga gccaactcgc cagccatata ggcggattac gcgcttcgtt ggccagcccg
      961 gcggaagttg atgaactcac aggctgtgtc ttcggcgcga tccccccttt cagcttccat
     1021 ccaaaactca aactggttgc cgaccctcta ctcttcgaac gatttgatga aatcgccttc
     1081 aatgcaggga tgctggataa atccgttatt ttgaaaaccg cggattattt gcgcattgcg
     1141 caaccagaac tggtcaattt ccgccgcact gcgtagctag cctatccgtt caataagcag
     1201 aacggacaaa accagcacaa ccgcaatagc aaaaaacgat gatgtgataa tcagtgtctc
     1261 gacgaacatt tgatcgttca tagcatgccc ccgggagata gctgttttca cgttattgtt
     1321 agcgtgcaca aatggcagtt tgatgacagt tcgcctgatt ttttatgcaa aaaacgtaaa
     1381 gattttctac ttccttcctg cagcaagcat aaagtaagca ggcttattat tttttggcaa
     1441 ggaaaccacg atgtttgatg tcactttgct gatcctgctc gggttagctg cgctgggctt
     1501 tatcagtcat aacaccactg tcgccgtttc aattctggtg ttgatcattg tccgcgtcac
     1561 accgttaagc acctttttcc cgtggattga aaaacagggg ctaagtatcg ggataatcat
     1621 tctgacgatt ggcgttatgg cccccatcgc cagcgggacg ctaccgccct caaccttgat
     1681 tcactcgttt ctgaactgga aatcgctggt ggcaattgca gtaggcgtta ttgtctcctg
     1741 gctgggtggg cgcggcgtga cgttaatggg cagccagccg caactggttg ccgggctgct
     1801 ggtgggcact gttttaggcg tagcgctgtt tcgcggcgta ccggttggcc cgcttattgc
     1861 cgcgggtctg gtttcgctga ttgtggggaa acagtagtta atcccgcaat atatcggcct
     1921 ggcgtttgcc ccaagccttt cttaaacata gtaataaagg ccgttgttga atcatacccc
     1981 agcgtatgag caactttctg tacagtatcg cctttcacta atccctgaag cgccataatc
     2041 agctgtaact gctgacgcca ctggcgaaaa ctcaaccccg tctctttcac tatcaggcgg
     2101 gcaagattgc gttcactcat cgcaaaaaag ccagcccatt gccccaatgc cccccactcg
     2161 acgggccctt tcgccatcat ctccaccatc gtgcggattt taggatgaga agaaacgggc
     2221 aggtgcagct gttgttgtgg ctgctgaggg agttcatcaa acaggacctg aattaatctg
     2281 cgggtcatcg gctcagcacg ctgagtcgtc gtcctgtttg ccagggttaa aatcaattcc
     2341 cggcataagg gcgatatttt caatgtgcaa catggtcggc attgtcactg cgctgggttc
     2401 gataaacaga aaacagagtt cagcgttagc tgtcacttga ttgctatgct tcaccccgcc
     2461 cggtatccaa accgcatatt gtggcggcac catccacaaa gcattttcca ccgtacaggt
     2521 aattgcacca tgtaatgcca gtatcagctg tcctttgcgg tgctgatgta caggactggt
     2581 taactcatcc gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt gtcggtgggt
     2641 tcatagccat tgagattcaa cctgtgcatc attttgtccg aacttagcga taatttgtca
     2701 ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg
     2761 agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg
     2821 gcattctgat gattgccaca acattacgcg tcacctttac cggcgcagcg ccgttactgg
     2881 atacgattcg ttccgcttac tcgctgacga cagcgcaaac cggcttatta accaccctgc
     2941 cattattggc ctttgcgcta atctcacctc tggctgcccc ggtggcgcga cgttttggta
     3001 tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca attcgttctc
     3061 tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag
     3121 gcaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta
     3181 ccggcgcata ttccctgaca atgggagctg cagcggcact gggatcggct atggtcgtgc
     3241 cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt catgctgatg tgttttcctc
     3301 tgctggctct ttttttatgg ctgccacagt ggcgaagtca gcaacatgca aatttgagta
     3361 cctcacgcgc cttacatact cggggtatct ggcgttcgcc acttgcctgg caggtaacat
     3421 tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgatcc
     3481 tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag
     3541 ccacagcagc tcccggtttg ctgatcgcac ttttcttaca tcatgtgaaa gaccagcgtg
     3601 gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc tgctttatgc
     3661 cagcgcacgc gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac
     3721 tggggttgac gttcattggt cttcggcaag ttctgcgcat caggcggcgg cactctcggg
     3781 gatggcacaa tccgtcgggt atttgctggc agcctgtggg ccgccgctaa tgggtaaaat
     3841 acacgatgct aacggtaact ggtccgtacc actgatgggt gttgcaatac tttcactact
     3901 gatggcgatt ttcggacttt gcgccgggag agacaaagaa attcgctaat atccggtgct
     3961 atagtgacgt gacaaatctt gcgtgaaagg gagaacaaac acgatgaata ttcagtgcaa
     4021 acgcgtttat gatccggctg aacagagcga tggttatcgc gtactggtcg accgcctctg
     4081 gccgcgcggt atcaaaaaaa ccgatttagc ccttgatgag tgggataaag aaatcacgcc
     4141 gtcaacggaa ctgcgcaaag cctttcacgg cgaagtcgtc gattttgcaa cctttcgcga
     4201 gcaatatcgc gcagaactgg cgcaacacga gcaagaagga aagcggctgg cggacatcgc
     4261 caaaaaacag ccgctgaccc tgctctacgc agcaaaaaac accacgcaga accatgcgct
     4321 ggtgctggcc gactggctac gtagcttgtg attttagtac ggcatccggc ggttattttt
     4381 caccagccgg atggtcccgt cgccacaatg cccattcatc aatcgtttca ccgcccggta
     4441 atttgcaatt gttgctgacc ccttgcgctg tctgcactgg aatgagcgtc ccgcccttct
     4501 gctggcaata gaccgacgcc ggatttgcca taccaatctg cggcggttta ggtgcttctg
     4561 gctgagaagg ggttgaacaa ccagccagga ccagcaagca aggcagaaca aaactgataa
     4621 ttttcattta ttgatctcac atatttatcc aagcttagag tatcgcggta tcgttttgtt
     4681 ttgcagcact atttttatta cattcactca aaacatatta cgtcttgttt catctttgtt
     4741 gatgatgttt tatcatgcct gcaaagatta aataatcagc acttacccgc cgtatcctgg
     4801 agttgttccg tgtcagatca gattatcgcc cgcgtctcgc aatcccttgc caaagaacag
     4861 tcactggaaa gtctggtccg acagcttctg gagatgctgg aaatggtcac tgatatggaa
     4921 tcaacctacc tgaccaaagt ggatgtcgaa gcgcgcctgc agcatattat gtttgcccgt
     4981 aacagccaga aaatgcacat cccggagaat tttaccgtct tgtgggatta ctcgttatgc
     5041 aaacgcgcca ttgatgaaaa ctgctttttc agcgatgaag tccctgaccg ttggggcgac
     5101 tgtattgcgg cacgcaatct tggcatcacc acatttctga gcacgccaat tcacttaccg
     5161 gatggatcat tctatggcac gctttgcgcc gccagcagtg agaagcgcca gtggagtgaa
     5221 cgcgcggaac aggttttaca gttattcgcc ggactgattg cacaatatat tcaaaaagag
     5281 gcactggttg aacagctgca cgaagccaat gctgcgctga ttgcgcaatc gtataccgac
     5341 tcgttaaccg ggctaccgaa tcggcgggcg atttttgaaa atctgacgac gctgttttcc
     5401 ctcgcccggc atcttaacca taagataatg atcgcgttta tcgatctgga taacttcaaa
     5461 ttaatcaatg atcgttttgg tcataatagt ggcgatctgt ttctcattca ggttggcgag
     5521 cgccttaata cgctccagca aaatggcgaa gttattggtc gtctcggtgg tgatgagttt
     5581 ttggttgttt cactaaacaa cgagaatgcg gatatttcgt cgctgcgaga acgtattcaa
     5641 cagcaaatac gtggagaata tcacttaggt gatgttgatt tgtattatcc cggtgccagt
     5701 cttggcatag tagaagtcga tcctgaaaca accgatgcag acagtgccct gcatgctgcc
     5761 gatattgcga tgtatcagga gaaaaaacac aaacagaaaa caccttttgt cgcgcatcca
     5821 gcgctacatt cctgaggcgt attcacatcc ttttgattgg tgataacat
//
LOCUS       DAJTRM010000171         5847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRM010000171 DAJTRM010000000
VERSION     DAJTRM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.173"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            433..1341
                     /locus_tag="OO322_003924"
     CDS             433..1341
                     /locus_tag="OO322_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5908588.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO322_003925"
     CDS             complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO322_003925"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5908589.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFEAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3656..4414
                     /gene="loiP"
                     /locus_tag="OO322_003926"
                     /gene_synonym="yggG"
     CDS             3656..4414
                     /gene="loiP"
                     /locus_tag="OO322_003926"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5908590.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OO322_003927"
     CDS             complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OO322_003927"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5908591.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5676..>5847)
                     /locus_tag="OO322_003928"
     CDS             complement(5676..>5847)
                     /locus_tag="OO322_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908592.1"
                     /translation="ADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCSGQAFPA
                     DNNGYAPPPCRK"
BASE COUNT         1436 a         1526 c         1520 g         1365 t
ORIGIN      
        1 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
       61 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
      121 atgaaagcaa ttgggtacgc gagaagaaaa accccacaaa ccaaaataat aaaaccttaa
      181 ataacaacaa tatgaaattg atatttttaa agtcaacttt gtatttgtta tgttcatcac
      241 aaaatcaaca ttttgtttgt tattgtattt gtgatgcata tctctattta tttattgaat
      301 gttcgaaaac atacttttct aatgcaatgt tttttcgcta taccccttta tagattctga
      361 attctcacct gtcggctgtt ttctcctcat ctcatgtgac atattcccat actttactca
      421 aggaagattg tcatgaaagc acctgtttca ttaacctcat ttcgcccaca aaaatctctt
      481 gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt
      541 aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg
      601 gggctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag
      661 gctgtggtct atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca
      721 gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac cacctacaac
      781 actattgaag cattaaaaga tcgtctaatt gtccgtcagt tgccaactca gctggagaac
      841 attttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaatt ggttcaggat
      901 ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg taattgacgg ggtccagatt
      961 gaaaatattg atttttctga cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa
     1021 gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg
     1081 gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagcggaa
     1141 actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa
     1201 gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata atcccggact tgtagccctc
     1261 accactgccg aacgttggga cggtaaactg cccgatacca tgatccccgg cagcacagtt
     1321 ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gtccttctca caaaacgtct
     1381 tactgattac agcagcgctt ttgctttcgc aacaacgtta tcaacagtga agccgaactc
     1441 ttcaaacagc agctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcgcc
     1501 gttcaggcca acatacttgt accagtagtc agcaataccc gcttctaccg caacgcgtgc
     1561 agtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt caaatgcgtc
     1621 ggtagacggc atggacacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc
     1681 agcaaccgcc agttcaactt cagaaccggt ggcgatgaag atcagttccg gctgaccagc
     1741 acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc
     1801 ctgctgcgcc aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac
     1861 gccgtatttc cacgcgaccg cggattcaac ctggtcacac ggacgccatg tagacatgtt
     1921 cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc catcttcgcc
     1981 cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc tgtttcatca gcgcagccat
     2041 acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt acggcaggaa
     2101 gccaccgtgc agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta
     2161 gtggatgtag ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag
     2221 gttagacggt gccaggtcag cagagccgcc gaggaattca ggcaacagcg ggccgaacgc
     2281 ttcgatagca ttctgcgacg ctttacggct ggcgattttc gccggattag cctgcagttt
     2341 agcgataaac tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg
     2401 ggtaaattca gccgcttcct gcggataagc tttcgcgtaa gcctcaaact tctcattcca
     2461 tgcagattct ttcgcctggc ctgcttcttt cgcatcccac tgagcataga tttcagacgg
     2521 gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc
     2581 gcccagcggc gcaccgtggg agtcgtgagt accggctttg ttcggggaac cgaaaccgat
     2641 gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttcaactgc
     2701 gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc
     2761 ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca acgtgaccat cgatagaaat
     2821 accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca
     2881 aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg
     2941 gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc
     3001 cataccgact gcgttggcaa taccctgacc cagcggaccg gtggtggttt ccacaccagc
     3061 ggtataacct acttccggat ggcccggagt tttagagtgc agctgacgga agtttttcag
     3121 ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc
     3181 gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg
     3241 tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg
     3301 gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag
     3361 ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac
     3421 ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc
     3481 gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg
     3541 ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt
     3601 cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa
     3661 aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt gccagaatat
     3721 ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga
     3781 tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca aggcgacgat
     3841 tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg cgctaggcaa
     3901 caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt
     3961 tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga tgacagataa
     4021 cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa
     4081 agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg
     4141 gattgtcggc agtttatctc aatcacaact tggtgatctg ggcgagaaat tagtcaattc
     4201 gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc ttctgcacca
     4261 acgcggcatc agcccggcag gtcttgccac cagctttgaa aaactggcga agctggaaga
     4321 aggtcgtcaa agctcaatgt ttgacgacca tcctgcatcc gccgaacgtg cccagcatat
     4381 tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta gtggtgtcga acgtaggtca
     4441 gataaggcgt tcacgccgca tctgacatta atggcacatt tgttaccttg tgcgcattgc
     4501 cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg tcggataagg
     4561 cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc atctggtgct
     4621 tactcgccct ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc
     4681 agagcggtga tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg
     4741 atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt caggccgcca
     4801 atcactggcg taccggttcc cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc
     4861 aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgctg
     4921 cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaactcggta
     4981 cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag
     5041 aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag
     5101 tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca
     5161 aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc
     5221 gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag atcgccgcag
     5281 tccacgacgt tcagacgctc acgcatgtcg aaattccacg ggaagcggtt gtgttcccag
     5341 gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc cgcacgacca
     5401 gaagtggcca tatcgaacgg cacgccagta atcacccagt ctgcatcgct gtcatacggc
     5461 tggaagttca tcggcaggcg taaaaaacca aaggcattgg aaaccagtga gttatcgtat
     5521 tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt
     5581 ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt
     5641 atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca
     5701 tagccattgt tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc
     5761 gggccttgta attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc
     5821 gccgaattgg ctgcgtagga atcagca
//
LOCUS       DAJTRM010000172         5800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRM010000172 DAJTRM010000000
VERSION     DAJTRM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.175"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            58..576
                     /gene="mddA"
                     /locus_tag="OO322_003929"
     CDS             58..576
                     /gene="mddA"
                     /locus_tag="OO322_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5908593.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            573..1022
                     /locus_tag="OO322_003930"
     CDS             573..1022
                     /locus_tag="OO322_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5908594.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1023..1256)
                     /locus_tag="OO322_003931"
     CDS             complement(1023..1256)
                     /locus_tag="OO322_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5908595.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1342..1515)
                     /gene="ortT"
                     /locus_tag="OO322_003932"
     CDS             complement(1342..1515)
                     /gene="ortT"
                     /locus_tag="OO322_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5908596.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1710..1805
                     /gene="yncL"
                     /locus_tag="OO322_003933"
     CDS             1710..1805
                     /gene="yncL"
                     /locus_tag="OO322_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5908597.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2207..3016)
                     /locus_tag="OO322_003934"
     CDS             complement(2207..3016)
                     /locus_tag="OO322_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5908598.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3226..4650)
                     /gene="patD"
                     /locus_tag="OO322_003935"
     CDS             complement(3226..4650)
                     /gene="patD"
                     /locus_tag="OO322_003935"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5908599.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4672..5466)
                     /locus_tag="OO322_003936"
     CDS             complement(4672..5466)
                     /locus_tag="OO322_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5908600.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5456..>5800)
                     /locus_tag="OO322_003937"
     CDS             complement(5456..>5800)
                     /locus_tag="OO322_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5908601.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1473 c         1286 g         1519 t
ORIGIN      
        1 cgggctgttt tcgatagtat tgataacatc atcatcatta ttttattgag gccagacatg
       61 tccatccgtt ttgcccgcaa agccgactgt gctgccattg cggaaattta taaccacgcc
      121 gtgttgtata cggcggctat ctggaatgac caaacggtgg atgcagataa ccgcattgcc
      181 tggtttgaag cgcggaccat agcaggttat ccggtgctgg taagtgaaga agatggcgta
      241 gttacgggat atgcctcgtt tggcgactgg cgtagtttcg atggttttcg ccataccgtg
      301 gaacattcgg tttatgtcca tcccgatcat cagggcaaag gtctggggcg taaattgtta
      361 agtcgattga ttgatgaagc gcgggattgc gggaagcatg tcatggtcgc cgggatcgaa
      421 tcgcaaaatc aggcttcgct gcatctgcac cagtcgctgg gatttgtcgt caccgcacaa
      481 atgccgcagg taggcactaa atttggtcat tggctggatc tgacatttat gcagttgcaa
      541 ctcgacgagc gcactgaacc ggacgcgatt ggatgaatca gtcgctcacc cttgcttttt
      601 tgattgccgc cggaattggt ctggtagtcc agaacacgct gatggcgcgc atcacccaga
      661 cctcctctac cattctcatc gccatgttgc tgaactcact ggtggggatt gtgctctttg
      721 tttctatttt atggtttaaa caaggcatgg cggggtttgg cgaactggtg tccagcgtgc
      781 gctggtggac acttatacct ggcttgctgg gttcgttttt tgtcttcgcc agtatcagcg
      841 gatatcagaa tgtgggagcg gcgacaacta ttgcggtact ggtcgccagc cagctgattg
      901 gcggtctggt gctggatatc ttccgcagtc atggcgtacc actacgggcg ttgttcggcc
      961 caatatgtgg cgcaatttta cttgtggttg gcgcctggtt ggtggccaga cgctcatttt
     1021 aatcagagga tggtgccgcc tttggttaac tgttcgtggc gcgcttccat atcttcttta
     1081 tgcttgcgac cgtgatgagc aatagcggtg cgcaaacgct gctgctgggt gtagcgatct
     1141 tcccgactta actccgcgtc atcgctcagg gcgattaata attcgttcat atgggcaatg
     1201 acgctctctt caatcgcggc atccacgcgc gcgatgactt catccagatg cgacatactt
     1261 tctccttagc cacttgtgaa atttatcgat taatccaaat gatataagtc tgcgtggtgc
     1321 cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac agcgccaccg ggaaactcat
     1381 cggccatgtg gcccccacca gaaaggcgct taagaaacgg atgcgtttct tatcgtgaga
     1441 aagaaaccag gtgataagaa atgcgattgc tgccataacc gcataaaaaa caagcatatg
     1501 ttgatagaga aacatgtacg ggcgtccagc catatgaata aaacgcgcgc aaatatgcgc
     1561 cattttttga cgtgcatcaa gttttttcac cagtttgaga aaaaatcatc aaacagcaac
     1621 ttgcccttat ccatcttcag aatgagtatt attgcggatt ttcttaaccc gtactataca
     1681 cataggggca atgataaaag gtggcaaaaa tgaatgtttc cagtagaact gtagtactga
     1741 taaatgtctt tgctgctgtt ggtttgttta ctcttatctc tatgagattt ggctggttta
     1801 tttgatgtag tgattttgca cgcagcacgt actgtcagag aaatcaactc tctgacagta
     1861 cgcaagatct tctcatactc tccaagtttg caacattatc cttaatattt acttcttttt
     1921 gctggcatct acattgaaat gagtagaaaa catcccccta ctgtcgtcta tacaaacagc
     1981 ttactgattc aaacggaatc ttgttttcac atttttctat cacgaatact ttatacgcgc
     2041 gggagtctat aaatgcttta tactttcaag gaataacgta aatataaaaa gatggcgtaa
     2101 agtatatgca tggcaggatg tttgcaacct ccttgatgac gctgccccat tgaaattgca
     2161 aaccccgccc tggttatctt gcccatttat catgcaaaac agataattat aggcctctga
     2221 atacctcaga aagattgaat tttttatagt ccctctcttg aatatttcca tttgatatca
     2281 atcgattcat ctcttcaaca aaataattta tatcatcaca cttattatta tccggaagat
     2341 ctgagtagtt ataattccac cattttattc tcaacagttc atcaataaca tttgaatcaa
     2401 acctaaatct gattattttt gcaggaactc ctgcaactat ggcatatggg ggcacatctt
     2461 tggtaacaac ggaattggca gcaataacag caccatcacc tattgcgatg ccacctttca
     2521 atacaacatc atttcctatc cagacatcat ttcctataat cggattttca ggtttggtaa
     2581 tgaatggttt caatgtccag ctacgttcaa attcactcat agcaaactta tcaaattcac
     2641 cgttataagt catcatgtga gtagtaaaac gattcaaagg atgatttcca cccattattt
     2701 ttacatttga tgcaatacta caaaatcgac ctatacgtat attccctgaa aaatggcagg
     2761 tcggaaatga aaaagcgccg acactggaga aatttttcac aggcattgtt gaatattctt
     2821 cagcaataac attatcttca attgttatct tgtcaccttt tgagaaaaga aataaattcc
     2881 tttctttata cgacttcttc cccttatgat ttaaaaagat tttattatcc atacaaaact
     2941 caaagtgttt tttcgtccat ctgaaggtaa gggttttatt tttatcttcc gtgaatttgt
     3001 acaattgaac tttcattgtt aatacttcta cttataaagt attagtttaa taacgatact
     3061 ctaaagcctt atgctaatca actgtgacag gatgaaaaat cgccatcaaa catttagcct
     3121 aatttttacg ccagttatta atgtaatcat tctaatactt aaccgccatt aaactcagaa
     3181 cctaaaacga tagtggccgt cctataacag ccactaacat ctaacttaat gtttaaccat
     3241 gacgtggcgg acgacggtgt aatcctccag cccataaagt gacatatcct tgccgtaacc
     3301 agaaagtttc tgcccaccgt gcggcatttc acttaccagc atgaaatggg tattgaccca
     3361 ggtacaacca tattgcagcc gtgcgctgac gcgatgcgcc ctgcccacat ctttcgtcca
     3421 taccgaagat gcaagtccgt actggctgtc attcgcccag ttcaccacct gttcttcgtt
     3481 gtcgaagggc gtaacactca ctactggacc aaatacctct ttttgcacga tggcatcgtc
     3541 ctgtaatgcg ccagccagca gcgtcggcgc atagtaatag ccattaccct tgcgcttttc
     3601 accgccagtg atcactttga tgtgccctgt cgctttcgcc tcttctactg ccttgctgac
     3661 gcgttcgaga tgcgccagcg agcttaaagg tccaagctcc gtagactcgt catccggcgc
     3721 accagatttt aacgttgcca ccgcagcacc cagtttttcc accagcgtat cgtaaatgcc
     3781 tttttgcgcg tagatccgac aagccgcagt acaatcctgt ccagcattgt aatagccaaa
     3841 tgtacgtaca ccttcgacca ctgcttcaat atccgcatca tcaaaaacaa tcactggcgc
     3901 tttgccacca agttccatat gagtacgctt aatggacgac gcggtatggc tgatgatgtg
     3961 ctcgccggtg gcgatagagc ccgtcagcga caccatccgc actttgggat gaccggtcag
     4021 cggatcaccc accgttttgc ctctgccaaa cagtatgtta atcacgcctg ccgggaagat
     4081 atctttcgcc agctctgcca acttcaacgc ggtcagcggg gtaatttctg atggtttaag
     4141 cactacgcag ttccctgccg ccagcgccgg agcaagtttc cacgcggcca tcatcagcgg
     4201 ataattccac ggtgcgatag aagccacgac ccccaacgga tcgcgacgga tcatcgaagt
     4261 atgaccttca agatattcac ctgccgccag accattcaga cagcgcgccg cacccgcgaa
     4321 aaagcgaaaa acatcgacaa tcgccgggat ttcatcattg aacgcactat gcagcggttt
     4381 gccacaatta cgggattcca gttcggcaaa aacctgacca ttttcttcga taacatccgc
     4441 cagtttcagc aggcattccg cacgcgcttt cggtgtggtt tgcccccatt cagcaaatgc
     4501 tgcatctgcc gcacgcacag cagcatcgac ctgctctgcg gatacctcgg caatctccag
     4561 taaaacgtcc cccgttgccg gattatagac aggctgtttt tccccttcgc cgctaacccg
     4621 ttctccgtta atcagtaact tatgttgcat agcttattcc tgtatcagtg gttattgacc
     4681 attgtcgcct tcgcgggtta gccaccaggc ccccaggatc ggcaaggttg ttaccaacat
     4741 aaccagcagt gccaccacgt tggttaccgg tacatcacgc ggtcgcccaa gctgattgag
     4801 caaccacaac ggtaacgttc gttcatgacc tgccgtaaag gtcgtaacga tgatttcatc
     4861 gaacgacaag gcaaacgcca gcattcctcc tgccagtaac gccgaactga gattcggcaa
     4921 cactacgtag cggaaggttt gccagccatt ggccccaaga tccattgacg cctcaaccag
     4981 actccacgag gtgcggcgaa aacgggcgat gacattgtta aacaccacca ctacgcaaaa
     5041 agtcgcatga ccgaccacga tggtgaaaaa tcccggctcc agattgatgg ttttaaaggc
     5101 ggttaatagc gccagaccag tgacaatgcc cggcagcgca atgggcagca gcaataacag
     5161 cgaaatggcg tttttgccga aaaagtctcg tctccacagc gcggcagctg ccagcgtccc
     5221 taacaccagc gcaattaatg tcgccagcgc cgccacttta agtgacagcg tcacggcatc
     5281 aagaatatca ctacgctgtg ctgccacgct aaaccagcgc agcgtcaggc cctgcggtgg
     5341 aaaactaaac gccgcatctt cagtgttaaa cgcataggcg gcgattatca ggatgggaaa
     5401 atgtaggaaa acaacgccgc cccaggccgc cagtttgagg aaaaacggtg cgcgttcaga
     5461 gtgcatcgaa cgctcccaga cgtttcacga acgccaggta cagtgcgatg agaataatcg
     5521 gcaccagggt gaatgccgcc gccatcggca tattgccaat cgccccctgc tgggaataaa
     5581 ccatattgcc gataaaatat cctggaggac caaccagctg cgggacgata aaatcgccca
     5641 gtgtgagtga gaaggtaaag atagagccag cggcaatacc cgggattgcc agcggcagca
     5701 ccacatagcg aaaggtttgt cgtggacgtg cgccgagatc agccgacgcc tgcaacaatg
     5761 acggcggcaa acgctcaagc gccgcctgaa cgggcaggat
//
LOCUS       DAJTRM010000173         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRM010000173 DAJTRM010000000
VERSION     DAJTRM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.176"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..411
                     /locus_tag="OO322_003938"
     CDS             <1..411
                     /locus_tag="OO322_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908602.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO322_003939"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO322_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO322_003940"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO322_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5908603.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJTRM010000174         5590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRM010000174 DAJTRM010000000
VERSION     DAJTRM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.177"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..279)
                     /locus_tag="OO322_003941"
     CDS             complement(1..279)
                     /locus_tag="OO322_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908604.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO322_003942"
     CDS             220..405
                     /locus_tag="OO322_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908605.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO322_003943"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO322_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5908606.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO322_003944"
     CDS             complement(1254..1670)
                     /locus_tag="OO322_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908607.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO322_003945"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO322_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO322_003946"
     CDS             1837..2862
                     /locus_tag="OO322_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5908608.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO322_003947"
     tRNA            complement(2915..3004)
                     /locus_tag="OO322_003947"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO322_003948"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO322_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5908609.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO322_003949"
     tRNA            3996..4071
                     /locus_tag="OO322_003949"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OO322_003950"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OO322_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAJTRM010000175         5492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRM010000175 DAJTRM010000000
VERSION     DAJTRM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.178"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            46..2607
                     /gene="mutS"
                     /locus_tag="OO322_003951"
     CDS             46..2607
                     /gene="mutS"
                     /locus_tag="OO322_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5908610.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2713..3365
                     /gene="pphB"
                     /locus_tag="OO322_003952"
                     /pseudo
     CDS             2713..3365
                     /gene="pphB"
                     /locus_tag="OO322_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3416..4183)
                     /locus_tag="OO322_003953"
     CDS             complement(3416..4183)
                     /locus_tag="OO322_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5908611.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4379..5287
                     /locus_tag="OO322_003954"
                     /pseudo
     CDS             4379..5287
                     /locus_tag="OO322_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5284..>5492
                     /locus_tag="OO322_003955"
     CDS             5284..>5492
                     /locus_tag="OO322_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5908612.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALS"
BASE COUNT         1344 a         1417 c         1442 g         1289 t
ORIGIN      
        1 aaaccatcac accccattta atatcaggga accggacata accccatgag tgcaatagaa
       61 aatttcgacg cccatacgcc catgatgcag cagtatctca agctgaaagc ccagcatccc
      121 gagatcctgc tgttttaccg gatgggtgat ttttatgaac tgttttatga cgacgcaaaa
      181 cgcgcgtcgc aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg
      241 atcccgatgg cggggattcc ctaccatgcg gtggaaaact atctcgccaa actggtgaat
      301 cagggagagt ccgttgccat ctgcgaacaa attggcgatc cggcgaccag caaaggtccg
      361 gttgagcgca aagttgtgcg tatcgttacg ccaggcacca tcagcgatga agccctgtta
      421 caggagcgtc aggacaacct gctggcggct atctggcagg acagcaaagg tttcggctac
      481 gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg aaccggccga ccgcgaaacg
      541 atggcggcag agctgcaacg ctctaatccg gcggaactgc tgtatgcaga agatttcgcc
      601 gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg ggagtttgaa
      661 atcgacaccg cgcgccagca gttgaatctg caatttggca cccgcgatct ggtcggtttt
      721 ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt gtctgttgca gtatgcgaaa
      781 gatacccaac gcacgaccct gccgcatatt cgttctatca ccatggaacg tgagcaggac
      841 agcatcatta tggatgccgc gacacgtcgt aacctggaaa ttactcagaa cctggcgggc
      901 ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg tcacgccgat gggtagtcgt
      961 atgctgaaac gctggctgca tatgccagtg cgcgataccc gcgtgttgct tgagcgccag
     1021 caaactattg gcgcattgca ggatttcacc gccgagttgc agccggtact gcgtcaggtc
     1081 ggcgacctgg aacgtattct ggcacgtctg gctttacgaa ctgctcgccc acgcgatctg
     1141 gcccgtatgc gccacgcttt ccagcaactg ccggagctgc gtgcgcagtt agaaactgtc
     1201 gatagtgcac cggtacaggc gctacgtgag aagatgggcg agtttgccga gctgcgcgat
     1261 ctgctggagc gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg tggtgttatc
     1321 gcatcgggct ataacgaaga gctggatgag tggcgcgcgc tggctgacgg cgcgaccgat
     1381 tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac gctaaaagtt
     1441 ggctttaatg cggtgcacgg ctactacatt caaatcagcc gtgggcaaag ccatctggca
     1501 cccatcaact acatgcgtcg ccagacgctg aaaaacgccg agcgctacat cattccagag
     1561 ctaaaagagt acgaagataa agttctcacc tcaaaaggca aagcactggc actggaaaaa
     1621 cagctttatg aagagctgtt cgacctgctg ttgccgcatc tggaagcgtt gcaacagagc
     1681 gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg cggaacgggc ctataccctg
     1741 aactacacct gcccgacctt cattgataaa ccgggcattc gcattaccga aggtcgccat
     1801 ccggtagttg aacaagtact gaatgagcca tttatcgcta acccgctgaa tctgtcgccg
     1861 cagcgccgta tgttgattat taccggcccg aacatgggcg gtaaaagtac ctatatgcgc
     1921 cagaccgcac tgattgcgct gatggcctac atcggcagct atgtaccggc acaaaaagtc
     1981 gagattggac ctatcgatcg catctttacc cgcgtaggcg cggcagatga cctggcttcc
     2041 gggcgctcaa cctttatggt ggagatgact gaaaccgcca atattttaca taacgccacc
     2101 gaatacagtc tggtgttaat ggatgagatc gggcgtggaa cgtccaccta cgatggtctg
     2161 tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga ttaaggcatt gacgctgttt
     2221 gccacccact atttcgagct gacccagcta ccggagaaaa tggaaggcgt cgccaacgta
     2281 catctcgatg cgctggagca cggcgacact atcgccttta tgcacagcgt gcaggacggc
     2341 gcggcgagca aaagctacgg tctggcggtt gctgctctgg cgggcgtacc gaaagaggtt
     2401 attaagcgcg cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa cgccgccgcg
     2461 acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc cagaagaaac ttcgcctgcg
     2521 gtcgaagcac tggaaaatct cgacccggat tcactgactc cgcgtcaggc gctggagtgg
     2581 atttatcgct tgaagagtct ggtgtaataa caattcccga tagtcttttg ctatcgggaa
     2641 tattaacgac aactgacgaa tcaaataaaa ataccctgta taataggaaa gcttatttta
     2701 cagggtaaaa ccatgccatc tacacgctac caaaaaatca atgcccatca ctatcgccat
     2761 atatgggtcg ttggtgatat tcatggtgaa tatcagttat tacaatcccg cttacatcaa
     2821 ctctcttttt tccccgaaac cgacttactt atttctgtcg gcgataatat tgatcgtggg
     2881 ccggagagtc ttgacgtcct gcgcctgcta aaccagccct ggtttatctc cgttaaaggc
     2941 aaccacgaag caatggcgct ggatgcgttc gcgaccggcg atggcaatat gtggcttgcc
     3001 agcggtggtg actggttttt cgatttaaat gattgcaaca agaggcaata gttctgttgc
     3061 tgaaattcca tcaccttcca catattattg aaatcactaa cgacataata aaatatgtca
     3121 tcgcacatgc agattatccg ggtgatgaat atctctttgg caaagaaata gcggagagcg
     3181 aattactctg gcctgtttat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
     3241 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
     3301 accagattta tattgatacc ggatcgccga aaagtgggcg gctgtcattt tataaaataa
     3361 ggtagtctca tgcttcttct gtgaagcatg agtaaccaga tgttatttca ggccattatt
     3421 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
     3481 gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
     3541 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
     3601 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
     3661 acgatgcaga aataaaagcc tgatcaatca tcaggctgca cagcatggtc gcagcggctt
     3721 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
     3781 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
     3841 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
     3901 aacttcccgg cagaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
     3961 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
     4021 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
     4081 tcatatgtga cacattaatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     4141 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     4201 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     4261 ttcacataaa ataacacaaa atgttaattt atgtgaatca tctcaccata tcgttatctc
     4321 ccagcgctta tgttcacaat atcaaacaaa atatcactta aattaacaag gagagcagat
     4381 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     4441 agcactgtca tgtgtccgcg caggtttttc tacctggggc gcagacctga acagcaatgc
     4501 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     4561 cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg gcctaggtta aacaggtgct
     4621 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     4681 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     4741 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     4801 catggcctcc ggtagcgata ttgcctttga acgactggta cccgtgctgg aagccgttgc
     4861 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     4921 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     4981 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     5041 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     5101 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     5161 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     5221 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     5281 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     5341 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     5401 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     5461 gccacacagc aatcgctggc ggctctgagc tg
//
LOCUS       DAJTRM010000176         5492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRM010000176 DAJTRM010000000
VERSION     DAJTRM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.179"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..223
                     /locus_tag="OO322_003956"
     CDS             <1..223
                     /locus_tag="OO322_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908613.1"
                     /translation="VTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTVGKRE
                     HKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(220..1161)
                     /locus_tag="OO322_003957"
     CDS             complement(220..1161)
                     /locus_tag="OO322_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5908614.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1173..1662)
                     /locus_tag="OO322_003958"
                     /pseudo
     CDS             complement(1173..1662)
                     /locus_tag="OO322_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1666..2886)
                     /locus_tag="OO322_003959"
     CDS             complement(1666..2886)
                     /locus_tag="OO322_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5908615.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2890..3633)
                     /locus_tag="OO322_003960"
     CDS             complement(2890..3633)
                     /locus_tag="OO322_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5908616.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3524..4990)
                     /locus_tag="OO322_003961"
     CDS             complement(3524..4990)
                     /locus_tag="OO322_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5908617.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4990..5223)
                     /locus_tag="OO322_003962"
     CDS             complement(4990..5223)
                     /locus_tag="OO322_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5908618.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5211..>5492)
                     /locus_tag="OO322_003963"
     CDS             complement(5211..>5492)
                     /locus_tag="OO322_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terL-like domain protein"
                     /protein_id="HCR5908619.1"
                     /translation="EADIVYDSIGVGASAGAKFSEINADRKSENAYARRVNYQRFNAG
                     AGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1172 a         1548 c         1386 g         1386 t
ORIGIN      
        1 cgttacgcgc gtctgtggct ttgccaacgg atacttccgc gtgcgcacga gtgaaccagt
       61 gcgtggcaac gtcttcctcc acaacatgac ggcctttaac aaactcgcgc cgagaaccgt
      121 cgggaagcgt gagcacaaac ggggtatgta cgtgtattac tgcatcattt tttgccatcg
      181 ggtcatcctt aatggccccg ccagggggcc atgtggctgt taaatgccat caacgtacga
      241 aatggtttct ttgtacactg gctcgactgc acccagcttg ccgtagtaag tgacgatctg
      301 atacagaccg cgatactgca ccggcacgct ctgaagcgga accagcgggt agcggacgta
      361 ttttttatcg ttggtgtacg caactatgcg atccttattc cccacaccac ggcctttcag
      421 ccatttaacc gcgcggatat tcagcggaac accgttctgg tgatagctga tggtgttggt
      481 ctgaaggttc gtcaacaggg actggttacc tgcagatgaa acgatgatgc tggacaacag
      541 agcaaactgc tcaggcggga tcagcaaatc acgcggaacc acagagtaac cagaagcggc
      601 ccacgcatca gacagcacct ggttaatgct tgcgcggatt tcgtccggtg ttgaggttgc
      661 ccacgttttg gcagcgttgt tgacaggcac gccgtccagg gtaacaaggc ctttcaggtt
      721 taatgcggaa tcgccaacat atacctgttc atcgttatcc atctgccatt tcagttgcat
      781 accgtcatac ttctgcgtat caatcgggcg gccgacctgc tgagcagcct gcaattctat
      841 gaccgtccag ccaagttcca tcccccacag gttcagcggg ttaccggatt tgccgatatc
      901 cacgttcacg ccagcaatag cggttgagtc tttgcctacc cagttttttc cattcggatt
      961 tgcgccagta cccgcagcgg cgaagctggt attcgtccag ctggaaatgt catctgcgat
     1021 ggaggcgtct tcacgcagtt gaatatcgcg gctccaggtg taccccacca gtggcaggtt
     1081 cagcgtctgg tcgagtctct ccagctcccc gatgagaaag gcaccagagc tgtcaacggt
     1141 tgcctgatca aaagtaatca ttcgtctgtt ccttaaatct tccaggaaat ttctgcattg
     1201 ccgtcagcat caccggcacc tgtgaattca gcgttggtca gcaccacgtt tttgccactg
     1261 actgacgtgg acatgaatcc acccagaggc actttgatgg attcatcagt ggagatcgcc
     1321 ttttttgata gtgctggcat caaaatcaga accgagatta acggtcacgt agccacgctt
     1381 catggcgtcg cccgggaagt tcttgcctgt tcccacctgg cgaaccatgt ccggctgcga
     1441 cgtggtcgga taagggcgca cgtagatccc cttcaccttg tctgcggtat caccatctgc
     1501 cagcggcacg aaaaaaccgt catcatcgta tttaccagcc aggccatagg cagcgaaggc
     1561 gttatcggat ttaaggacca ccggttcgac ggttaagtcc tgcgggcgag agacagcccc
     1621 ggcaatgcca acaggcatcc ggtacagaaa tacattattc attttttacc ctttacggtt
     1681 tgcccagaat tcagcgtttt gtttgttcag ggaagcgata ctggtcatgc ccatgtttag
     1741 gcgctgtgca tcgccggtgg tggcgcgggt gtttcgccct ttggcaacct cagacacggc
     1801 attaaacgcc atgtcgaccg attgtttcgg taatttgcgg atatccgcat caccgactat
     1861 ctggcgaacc agcgttttgt cagcagaagc cagaacctcg cgtttgaacg cggtcggttt
     1921 catcttacgg ctcagatcga tacccggaac gataacttcg gcacgccagg ctgagtcacc
     1981 agtaatcgtg gtttcctctt catcgtcctc gccgtcaccg gtcggattat cgtcaggctt
     2041 attatcgtta tcgcccgtgg catttctttc cagcttagcc agcagggctt tcagtaatgt
     2101 tttgaggtca tcatcactgt cgccggttgg atctccgccc atctctggtg ctttgtccgg
     2161 tagtggttgc tgcggggaca ggttgatgtt gagattaacg ccctgcggca aatccccctc
     2221 atctcctgta accgatgcgg gagccgactc caccagttcg ttcatggtgt cggcatctcc
     2281 tgtcttgatg gccgcacgca tgcggttcca ccagtttttc ttttgatttg ccattgtgtc
     2341 tctgtctcca attgcacaac gatttccggc tctgccttta gggacaagag ccacatggtt
     2401 tccggtaata tcgacctgct cggctttacc tggctcggtc tgctcgtact ccgcgtcata
     2461 gccgcacgac acttcgcgca ggccatcttc gataagctga atggcgcttt cgtctttgac
     2521 gataaggtca gccagcatca aatcagactg ctcacccgtc ccgcgccgga cattctggag
     2581 gtgcccgaca gcaagcactt tccagttctc gggatttacc agccgcacat tcccgttttc
     2641 atcttcagga tgcaggatcg tgatgctcat cccttcgaat gaggcaagcg tggccggatg
     2701 gaatacctgc tcaggagaac gcgtgacgac tatttcaacg aacttatcgg gtttcagttt
     2761 tggcaggtca tcagcaccat agagctgctt acctgttcgt cctatcggca cgtctttgca
     2821 cagcaacgag ccgtcagcca ggcggtatcg ggtttccccg agcctggtct caaaaaagta
     2881 tttcatgggt tatgctgccc ttttcattgc gaatggaacg cgaacaaaca cgatctcttt
     2941 atagcagcgg cagtttggca gctcgcccgc gtggccggtc atgccgtcca aagttggagg
     3001 tgaatcccag cggacgaatt tacctttcat gtgatcgtgg gaatctcgga catcgccatc
     3061 atcggctgta cgccaaatat aaccatccga gccaatagct atcgctcggg ccatatccag
     3121 cgcgcccgtt gctcttccca gttccgttcg ggcaatcaga tcggccctag acttagcaac
     3181 atcgccggta gatgcaatct ctttagcaaa ctgctccgcc cgaccaccgg tgacaacagc
     3241 ctctatcgct ttgttctgga tgtcgtacac cctgtctgcg gcctcgagag gcagagattt
     3301 gatgtacttg acctgttcgg cgatgatgga ttgcatcacc tggcccacag gagcgctttc
     3361 cacaagattg cggagctcgc gactgatgtt cttgctgtgt tgccgccaaa ctttctcgtt
     3421 ctgccgggtt aggtccgcag taaagttttc cgcgaccttt gtcgcccagg gggtgatgat
     3481 ttcactgtaa cgctccagcg ccgcaataat ttccgtgata ctgtcatttg aaccatcgta
     3541 gcgaccattt acgatgtctc cgaccgccct cgctatcctg cgtaggctgg ttcgataccg
     3601 gatttccgcc tgacggctcc tgcggttcgt catcaggttc gccgatgccg ggcggcgctt
     3661 catcttcggc attctcgatg tcctcgttgg taatggatgc ccctatgccg gttacgtcag
     3721 aattttctcg caaatcagtc atagcggctt tcagtgtcat cagaccatca cccagcgccg
     3781 tactgattgc gttggtggtg tttaacgcca ccgttgagcg atcgacatca gacatttgcc
     3841 agagcgggtt aaactcaaac gtgaaatcgt ccggcagcgg cttgccaagt tccgaacgat
     3901 gcatgatgtc cagtatccgc cgcaccggaa gacgtaaacg cctctcctgc aacgaactga
     3961 tgcgatcgta atagttggca aggtctgcat caccggtaga aaatcctttc ggggactgtc
     4021 cgaacaaccg caccagtggg ataccaacag cgccactaat ctgttctgca aactgcgaaa
     4081 ggatgtcatc cagaccactg aagctgtact gatgggtttc aaacttatcc cgcgagtcca
     4141 tgagcgtcat gccttcatta ctctggaact gtcgaatcag gtcgatattc ttcagcaacg
     4201 cttcatacgc aggaccacca agtgcgataa gctcgcgtag cttctccacg ctgtaggtac
     4261 gcaaatgcgc cttgtagacc agctgcgccg cgccgacagt agcgctgtcg aacgcggtaa
     4321 gacgatccca gatacgctct acaaccgaca ttccccattc gttctcggtc atcttctgct
     4381 gaaatggcaa cgtgacgcca tcaaagcgaa tcaggcgact gtgatgaatg cgccaggcag
     4441 gaattcccgt tgctgtggtc accacatcgt aaaactcagg tttacccagg tccggcccca
     4501 tatctttaat gcggcgggtc agtgccgggt cgaccatcca gcggtcgagc gggagaatcc
     4561 ccttaaactt gcccttaccg atggtttcgg gtcgcagcgg ggtcattggt gcctgcccct
     4621 caatcatgat gaaacccacc gcgccgccgt agaggcgcga ccatttcagc acgtcattca
     4681 gcgcatccca gatttgcaac tcatccagtt gtgattcgag aatgccacga tcttttgcat
     4741 caatttccga agtgatgcga atgcctttgc gggtcatatc atccgggata gcatcgactg
     4801 cttcgccgat gatccaggat gaacgatagg accattccac cagcatgcgg ttacgactgg
     4861 tgaaattagc ccggtaggtg gatgctgagt gctggttagg tgtctgcatc cctactcggg
     4921 caataaaatt ctcataacca tcagctgtgg cctgcacagt tcgccgcagg gcttgtttgt
     4981 ttcgtgccat caggcctgtc tccccagctg ttcccagata tccagcgatg tatcgattgg
     5041 cgcgaaggcc ataatgaatg catcagcaac gtttggtgac ggtatctcgc gttttgcgag
     5101 gtctttttta ctttcgacca tcacacgtcc gttacggtcg aaatcacgat gaggtgttgt
     5161 cagttccagt ttcagctttt caagcaacgg acaacgagaa tctatgctga tcagctcatc
     5221 cacaggatac tgttctccgt tgttaatggc gttaaacgta tttctgaaac ggtcagccac
     5281 catgcctgag ccttaagatt tgcgaaaaag tctttgttgg ggatgccgtt gtactcgtca
     5341 tctggctcat gcacaccagc accggcgtta aacctctggt aattcacacg tcgcgcgtat
     5401 gcgttctcgc tcttccggtc agcgttaatt tcagagaatt tagcaccggc agacgcacca
     5461 acaccgatag agtcgtaaac aatatctgct tc
//
LOCUS       DAJTRM010000177         5327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRM010000177 DAJTRM010000000
VERSION     DAJTRM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.180"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO322_003964"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO322_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5908620.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO322_003965"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO322_003965"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5908621.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO322_003966"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO322_003966"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5908622.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO322_003967"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO322_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5908623.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1285 c         1453 g         1279 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacga
//
LOCUS       DAJTRM010000178         5209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRM010000178 DAJTRM010000000
VERSION     DAJTRM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.181"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1352)
                     /locus_tag="OO322_003968"
     CDS             complement(<1..1352)
                     /locus_tag="OO322_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5908624.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     "
     gene            complement(1368..2252)
                     /locus_tag="OO322_003969"
     CDS             complement(1368..2252)
                     /locus_tag="OO322_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5908625.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2263..2868)
                     /locus_tag="OO322_003970"
     CDS             complement(2263..2868)
                     /locus_tag="OO322_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5908626.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3039..>5209)
                     /locus_tag="OO322_003971"
     CDS             complement(3039..>5209)
                     /locus_tag="OO322_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5908627.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1522 a         1282 c         1069 g         1336 t
ORIGIN      
        1 atctcttgcc cctgtgcaac atttaactca ctgtaatcac gtaacaaaat atcgcttacg
       61 gcaggctcgt ttgccagcgc atccagtaca tagcgtccat gccctgcggc aatgtcaacc
      121 acgcggacgg ctaaaccttt ggcgtggaga tcggcaacgg cttgtttaat caatatttgc
      181 aaatgggttt tgcgctggcg aataccgcgc catccaacac tgttcaggta gattttgtcg
      241 attaaacgcc cgaatgcgtt actgccttgc ggttgatttt gatagacata atccagggaa
      301 ctgcctgaat caaagccggt actcattccg agatgcaggc ccgcagagtg cgcaccgaac
      361 aatttcatcg cctttcgcat aaagcgatac gctaaatcaa ccggcgataa tggcacgggt
      421 ccaccagaaa ggagccgcca tcgatccgct gatggtcctg tgcggtcttc atgctgataa
      481 tcaaattttt gcgatttgtt agcatataag cggcagataa agctttgcat tttttcaaat
      541 gcctgcgccc tgttctcttc acccaatgtg tcgtgataaa agcctggcag cagatgcagc
      601 tctttcagag ggctacgtaa tctctgataa aaatcaatct gcggttggcg atgcaccaca
      661 tagtcatcac ctgatatcag aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca
      721 atacgttcag acgttttata gagatcgagc aagatattga cggcaatcgc ccgtgtgatc
      781 agcggatcat tattgaaact cgccacccgt tgccgatcgt gggtcaaata gcgccctttc
      841 acataggaat taataaaaaa tagaccacgc agacgatgcc ataacgccag cgcaggacgt
      901 gccagcggca catacaattt aaccttaaag gccggagaag ccagcaccag cccacgaatt
      961 gcaggtgcat aatcatgaac ccatgtggca acaagcactg cgccgacgct ttgcgcgatc
     1021 actaccacct cttccagtcc gacctggctg tcgctggcag caaaacggac aaattcatcg
     1081 acatcctgca ctgagcgcat aagagacggg ctataaccac gcggccccga attttttcca
     1141 tgcccccggg catcccaggc ataaaaagca gtatcaggca tcgccagttc atcaacgaga
     1201 tgttgtagac gaccagaatg ttcatgcccg cgatgaaata agacgatgac ctttttcgcc
     1261 cctggctgta aagtgggcca gtgccgataa tacaatgctg tattatcact ggtggtaaaa
     1321 aaatgttccc cagggatgcg tgaattttcc attttctcta ttttccttca gtaacagcag
     1381 tagcgaataa aataaaaaaa taccggagcg gtgaaaatta atgagtcaat cctgtcaagg
     1441 agtccgccat gtcctagtaa tagttttcca ctatctttaa caccaatatc tcgtttgatg
     1501 gctgacatca ctacatcgcc gcaaaaacca ctaataccaa ttaacaaacc cgctaataat
     1561 gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg
     1621 gtgattacgc cccccagcag accttccagc gtttttcccg ggctgacttt ggggaccact
     1681 tttcttctgc cgcaggattt cccccataaa tactgtgcaa tatcgtttga ctcggttaac
     1741 gccaaaagaa ataacaccag taacgccccg ccctggatat ttatggttgg caacattaat
     1801 agccaggcgg catgactcaa ggcaaaaacg gtcattatcc atccccaaaa aatggcgctc
     1861 acggtatgct gaaaaccgga ggggtcaccc acaaaaactc gccaggtggc taatatcaga
     1921 aacccagtca aaggtataaa taacagaaac agctcaaagc aattaaaacc gataagcaaa
     1981 tagttaagag gaataaccca atataaccaa cgcgaaaaat gtacggatat aagcgtacaa
     2041 tattctttca atgccagaaa acttatcagc gtaaaaaacg tcaacgtcat ccagcgtggg
     2101 cctgaaatca ccagtgaaaa acaaatgacg atgccccacc aggtacgaat acgtaatgta
     2161 acttcatccc cttttctctc aggtaaccgc catagcagaa aacggtttat cagcttgcta
     2221 aaattaacaa cgcgaaaagt gttgccagag atttttccag cattatcttt cagcgctcat
     2281 aagaagcaca ctccgacaac gattaatcgc agtacagata agcagaattg aagcaatgct
     2341 ccataaaaga ttattccatt gcatccactg tggataaatg gcaacggcca gaccccacag
     2401 accaaatatt aacgcgcgat cacttttgcc aaatggaccg gcataactgc gaacaccgtt
     2461 aatcgtctgc gcgagtaaac cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat
     2521 cacgagtgat gcgttacttt ccggtaaaaa taaaaacggt aaatagagcg caatatcgga
     2581 aataacatcg ccagtctcat tcaaaatcgc ccccagacgt gtttgctgat tgcactcacg
     2641 cgccaacatg ccatccagcg cattgagcgc catacggata aaaagcacga tgggcaatag
     2701 caaaaagagg atgggttgtg ccaccaacac caacagcaat ccggtaaaaa gagaaagcgc
     2761 cagtgccgta agagtgatgt gattcgctgt aacgtggtgc ttataaagcc aaaacatcgt
     2821 cggccttaac agcgactgaa agagcggttt tatctggtag agcgtcatta tcaatccttg
     2881 ataattcggt ttgcgctatg gtgcatcaat tcccctgacg gagatatcgc aacacgccgc
     2941 ttcattaaaa attcagaata tatcaaggta ttgggggctc actgtcagga atgattatcc
     3001 ccaataaatc aagtagttaa aaattattac gctgttattc aatacaactc atcaagtttc
     3061 ctcatcgctt gttcccgtat tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa
     3121 tccagttcag ggttaacaag ctctaacgcc agcaaaggat cacgtttcag cctcgccttc
     3181 aatacatcaa ctggaaagcg gggattattc atcgccacaa tccaccactc gggatcaatt
     3241 tctgccgcca gtgccgcaga gggggtgtgg cggttgctaa gtgcgccgaa cttgaaaaat
     3301 gtgagattgc ttttcgccag tgtactccac catatacgat ccggttgttt tgccagtaca
     3361 tccagttgtg ctaacgcatc ggggtcgagt gcataataaa cacgtaatgc ttcatcaacc
     3421 tgcgcacggg agctattttc ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc
     3481 gtgttttgct gtaattcctt gagaacaaca gcgggtaaat tatcccaact aactaatgca
     3541 ctctctaaaa agtcccagtc ttccaaacgt atcagggcgg cggcatttaa tagcatctgg
     3601 tcatcaactt cgccgtggct aatcagctca gacaaaatca tatcagcgct ggtttcatat
     3661 gcatctgaat ctgctttcgg attatttttc cgatgcgata aagcacgttg ccaaattttt
     3721 tgtttcatcg acgtgctcag atactgattt ttcgccagat tactccaccc ctcaggatcg
     3781 ttgaaacgtg tcaataaccg ttgtaaagca gcgtcagagt gaattacctg catatcccga
     3841 cagcaaaagt aggcagggga ttttttctcc atccttttaa caaaattaaa ctgtaagtta
     3901 tctggcaatg ctaaaaataa cacacgtatt agatcatcgt cattctcgaa tttttggtat
     3961 aaataggctg caactttgtc actctcttgt ggcgtcatcc gcaataattg accatggtgt
     4021 ttcatctctg ccagcttgaa cgctaagctt gtgacaacat tgtttgatga atcgttcgcc
     4081 aactgcattg cctgattgta agtgagttgt cctccagcac cgacctgacg aatagaggaa
     4141 tcatcactgc ggatatagcc atcaataatg gattcgggca ataaccacgc atctttataa
     4201 agctcctgaa tgatcatatc ccaatctctt ttcgccagag tcatgacgat atccagaggg
     4261 agattttcac gtagcgcaag attcaacgga ccgacaccat caggtagacg actcattcgc
     4321 gtcattattt cgacagagag cggttcacgg tcgagcaacc ccagaacaac aggctggtaa
     4381 cccaattgct gatcggtcaa ctccagaatc tcattgtcag gcatatatct cgccatactt
     4441 tcaatcgttt gttcatcatt gctattaata agttgtttgc gtaggttagc tgaaattcct
     4501 ttaagccttg ggagagttcg cattatggca agatcaaatg tcgcaatatc tcgtccttca
     4561 aatgcagcaa cgttgccttc atccagcatt tgcaattcct gcactaactc aggagaaggc
     4621 ttagggcgaa acagcgcttc ttttttcatc tgattacaga cattccctgc catgcgaata
     4681 ttgcttcgct taaaaatttc tttcagagca tcttcatcat ttattagacc ccaataatat
     4741 gcatcaggat ttattatcat cagcgctttt ttgacatttt cagtgtcatt tttgtcactc
     4801 gcacggacca tgaattcact atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg
     4861 ataaataccc cacgcgcatt aatactcccc ttaatcgtag acggattatc gtaatatgag
     4921 tcgataaata ccagtccact tgcatttatc gaaccggtaa ttaacagtgg tgaatccgta
     4981 actatattcg ttgccgtcac gttaccgaga ataacccatg gattagaaag agagaaagtg
     5041 gaaatattac ctttgactgt caaattgcca agaacaatca atttttgatt actctctata
     5101 ttaccttcta ccactaaatc gccacgatgc actttccagt ccgatgcaga taaaccgaag
     5161 gggacaagat tcacgctact gttgatagct tgttcaactt gttgtttat
//
LOCUS       DAJTRM010000179         5207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRM010000179 DAJTRM010000000
VERSION     DAJTRM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.182"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(226..384)
                     /locus_tag="OO322_003972"
     CDS             complement(226..384)
                     /locus_tag="OO322_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5908628.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            complement(368..679)
                     /locus_tag="OO322_003973"
     CDS             complement(368..679)
                     /locus_tag="OO322_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5908629.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(863..1753)
                     /gene="cyoE"
                     /locus_tag="OO322_003974"
     CDS             complement(863..1753)
                     /gene="cyoE"
                     /locus_tag="OO322_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5908630.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(1765..2094)
                     /locus_tag="OO322_003975"
     CDS             complement(1765..2094)
                     /locus_tag="OO322_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5908631.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2094..2708)
                     /locus_tag="OO322_003976"
     CDS             complement(2094..2708)
                     /locus_tag="OO322_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5908632.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(2698..4689)
                     /gene="cyoB"
                     /locus_tag="OO322_003977"
                     /pseudo
     CDS             complement(2698..4689)
                     /gene="cyoB"
                     /locus_tag="OO322_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            complement(4711..>5207)
                     /locus_tag="OO322_003978"
     CDS             complement(4711..>5207)
                     /locus_tag="OO322_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5908633.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
BASE COUNT         1391 a         1387 c         1295 g         1134 t
ORIGIN      
        1 acatgttcaa aaaaatcaca tagtaggtcc tgaccaaagc cccgcttttg atacttccgc
       61 gctcccccca acatgactaa tctgacaaca ccaatttctg aaggttgtga accctgaagg
      121 acgccagaaa cgcgttgtct ttccagcgaa tatgccgtaa aagtacagat gccaatcagt
      181 tcaccacttt gtctgtcaat aagtgcttta gccgcacagt cgctattacg cacacttttc
      241 tttagcgatg cgcgtagcgc gtacaaattt atcgataaca gggttaccgc aatcaaactg
      301 tttttgccca ggataagtaa tatcagactg ataattacag gagaggacta tgggcagagt
      361 aatctcttca tgcttgtcga ccatcgctgt ttttcctttt cattaacgcc ttgagggcga
      421 gcgtcggttg agcaggttca gcgatgagtt ggttcatcag ttcccagctt tgattcgaaa
      481 gctcacggcg acgttggtta tcaagcacac tttcggcgcg ttccatagca gcgctcaaaa
      541 taaaagcgct taaatctacc ccaacggctt gcgcagcttc acgaagtttg tttttggcgt
      601 cgggactagt tttgagttcg acccgcgcat ttttcagcgt tttatcgaca tcgtatagag
      661 cggctgtttt tccaggcatt atgacttctt tgattaactg aatgaaatcg tagggagtcg
      721 atctcataca taatacggct atcaaccgta tgattaattt atgtgcatct gctcactctg
      781 tcaagagata cggttattag ccgtatctct gatctcagac aataaaaagg tgccgtagca
      841 cctttttaat agagaggttt tgttaccaca cagcagccag cagcgtatgc gagtccggta
      901 ccataaaatc aacggacatc atcaccgaga gggcagtgat ggcgatgata gagaagccga
      961 acagcttgcg cgcccagatt ctgtcatcag caactttata accgcgcaga gccataccta
     1021 accaccagac gctaaccgcc gcggcgacca ccagatattt atacccagcg taaccgccaa
     1081 gagagagcat cagcgtggca acggcaaagg cgatgatata cagcgtgatg tgattcttcg
     1141 ccaccgaaat gccttttacc actggcaata ccggaatgtt tgccgcctgg taatccttaa
     1201 agcggaaaat ggcgatggca taggagtgag gcatctgcca caggctgaag atagccagca
     1261 ggatcgctgc gccgctatca aactcgccgg ttactgcaca gtagccaatc accgacggcg
     1321 cagcgccgga gagcgaacca atcaatgtgc cgtagactga gtggcgtttc atgtacaggc
     1381 tatagacgcc gacataaacc acaaagccca tcacccccag ccagcaggcc agcggattcg
     1441 cgccaaacca cagcagcata aagccagcaa tacccagcaa ggtggcgtac accagcgaga
     1501 cagcaggaga gatcaggcct ttaaccagca cccgattctt cgtcctttcc atctttctgt
     1561 cgatatccct gtcgatgtag ttgttaaaca cacaacccga cgccacaacc agtgacaccc
     1621 caaccagcgt gtagataaac aggggataat caatgctgcc ctttgaggcc agcaggaatc
     1681 ccccaatcac cgagatcagg ttgccaaaga tgatgcctgg tttcgttact tgcaggtatt
     1741 gcttaaacat cataaccgcc gctcttagtg catcatcatg ttgtagttga ggttccacat
     1801 aatccagatg gagcctacaa ccaggatagc gatgattagc acggtgaaga caaacgccgt
     1861 catgttccag ccttcatctg atttggtatt catgtgcagg aagcacacca gatgcaccag
     1921 aacctgtacc actgccattg ccaggattgt tcccagaatt acggccggag aggcagcccc
     1981 tgtcatcacc atccagaacg gaatcaccgt cagaatgatc gacaggataa agcctgtcat
     2041 gtaggttttt acgctgccat gggacgcgcc gctgtgatcg gtagaatgac tcattacatc
     2101 gcccccatca gataaacaac agtgaacaca cagatccaaa ccacatccag gaagtgccag
     2161 aacaggctca ggcacatgat gcgggtacgg ttagtgctgg tcaggccgcg acgggcgatt
     2221 tgcaccatca gcaccgccat ccagataaga ccagaagtga cgtgcagacc gtgcgtgccg
     2281 accagcgcaa agaacgctga caggaagccg ctgcgatccg gccccatgcc gttaacaatc
     2341 aggtgatgga attcatagat ttccatcccg ataaatccgg caccaaacaa ccaggtcaac
     2401 gccagccagg agataacctg gcttttgttg tttttgtaca tggcgatagc cgccatgccg
     2461 taggtgatgg agctgaacaa cagcaagaaa gtttcaacca gaacgaacgg cagttcgaaa
     2521 atgtccttac ctgtcgggcc gcctgcggtg ccgttcacca gaacggcata ggtagcaaac
     2581 aagattgaga acagaatgca gtcgctcatc aggtagatcc aaaatccgaa gattttggtt
     2641 ccgcctgcat cgtggtgccc gtgttcgtgc gcgtgggcag tcgcgtgcgt caaagtatca
     2701 gttgccattt ttcagccctg ccttagtaat ctcatcgaaa tgctggtttt ccagtttttc
     2761 gatttctgcc accggcacgt agtaatccac gtcctcgtcg aagcttttca cgatccaggt
     2821 gatgatcatg cctgcgaagc caacaatcgc cagccaccag atatgccaga tcatggcgaa
     2881 accgaagatg gtggagaaag ctgcaatgac gatacctgca ccgctgtttt tcggcatatg
     2941 aatttcttca tagtggtcag gctttttata cgcttcgcct ttctctttca tttcccagaa
     3001 tgcatcacgt tcgtgaacgt gcggcactac ggcaaagtta tagaacggag gcggggaaga
     3061 ggttgcccac tccagcgtac ggccacccca cgggtcgcca gtcaggtcac ggttctggtc
     3121 gcggtcgcga atagaaacgt acatctgaat aacgaggcag agaataccca gcgcaatcag
     3181 tactgcaccg ctggctgcaa tcatcagcat ggtgtggaac tgcgggtcaa tctgctggct
     3241 caaacgacgg gtcatgccca tgaagcccag cgcatacagc ggcataaagg caacgaagaa
     3301 gccgatgatc cagaaccaga acgcgcgttt accccaggtt tcgttcagtt tgaaaccgaa
     3361 cgctttaggc caccagtagg tcatccctgc gaagcagccg aagaccacgc cgccgatgat
     3421 cacgttatgg aagtgcgcaa tcaggaacag gctgttatgc agaacgaagt ccgcgcccgg
     3481 tactgccagc agcacgccag tcataccgcc caccgagaag gtgacgataa aaccgatagt
     3541 ccacagcatc gcagaatgga acacgatgcg gccctgatac atggtgaaca gccagttgaa
     3601 gatcttcacc ccggtcggga tggcgataat cattgtggtg ataccaaaga aggcgtttac
     3661 gttcgcgccc gcacccatcg taaagaagtg gtgcagccaa acgatgaacg acagcacggt
     3721 gatgcagacg gttgcccata ccagcgaggt ataaccaaac agacgtttac gcgagaaggt
     3781 tgccgcaatt tcggagaaca caccgaaaac aggcaggatc aggatgtaaa cttccgggtg
     3841 gccccaggcc caaatcaggt tgatgtacat catcatgttg ccacccatat cgttggtaaa
     3901 gaaatgggtg cccagatagc gatccagggt caacaacgcg acggtaaccg tcagaattgg
     3961 gaaggaagca ataatcagta cgttcgcgca cagtgatgcc taggtaaata ctggtatctt
     4021 gaacatggtc atgcccggtg cgcgcatctt cagaatggta acgaagaagt tgataccggt
     4081 aagcgtcgta cctataccgg atagctggag actccatatc cagtaatcaa caccgactcc
     4141 cggactgtac tctattcccg atagcggtgg ataggccagc cagccggtct gcgcaaattc
     4201 gcccacgccg agagaaacgt taaccagaat cacgccaaca acagtaaacc agaagcttaa
     4261 gttgttgagg aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa ccaccaggtt
     4321 catcagaccg ataacgaaag gcatcgccac gaagaagatc ataatcacgc cgtgcgcggt
     4381 gaagatctga tcgtagtggt gaggtggcag gaagcccgct tcacccgccg aggcaagagc
     4441 ctgctggcta cgcatcataa tggcgtcagc aaaaccacgc agcaacatca caatcgccac
     4501 gatgatatac atgataccga ggcgtttatg gtcgacggag gtcagccact ctttccacag
     4561 gtaggtccac ttaccgaagt aagtgatcag gccaacgagc gccagacctc ccaaaataat
     4621 gccagcgatc gtaaccatga cgataggttc atggaacggg actgcatcaa gtgataattt
     4681 tccgaacatc tttattcttc ctcaacccct ttaatgggcg gattccgcgt ggctcatgtc
     4741 catgccttcc ataccttcgt gtgcactgtg ctcaccttct ggctgggtca tgtccatgct
     4801 cttaccgtga gccataaact tgttaatcac atcggcaaac aagtctggtt tcacgttgga
     4861 gaaatattcc acctggttgt attcgctagg cgcggccagt ttttcgaacg cagccatgtc
     4921 agacatggtg ttcggcgact gtttcgcttt tgcgacccat tggtcgaatt cggcgcgatc
     4981 cggtgttgca atagctttga acttcatgcc tgagaagccc ggcccgctgt agctggcgga
     5041 gataccgtca taagtaccgg gttcgttggc gatcagatgc aggcgagtct gcataccggc
     5101 catggcataa atctggctac ccagacgcgg aatgaagaag gagttcatca cggagttgga
     5161 ggtcactttg aagtacaccg gagtgttcgc cgggaaagcg atttcat
//
LOCUS       DAJTRM010000180         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRM010000180 DAJTRM010000000
VERSION     DAJTRM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.183"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="OO322_003979"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="OO322_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5908634.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="OO322_003980"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="OO322_003980"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5908635.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="OO322_003981"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="OO322_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5908636.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="OO322_003982"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="OO322_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5908637.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO322_003983"
     CDS             4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO322_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5908638.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1255 g         1417 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ttggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
     4921 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
     4981 acgttatccg catatcagca tttgctggga acagagcgac acct
//
LOCUS       DAJTRM010000181         4946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRM010000181 DAJTRM010000000
VERSION     DAJTRM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.184"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..249
                     /locus_tag="OO322_003984"
                     /pseudo
     CDS             1..249
                     /locus_tag="OO322_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(299..1249)
                     /gene="uspE"
                     /locus_tag="OO322_003985"
     CDS             complement(299..1249)
                     /gene="uspE"
                     /locus_tag="OO322_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5908639.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1401..2153)
                     /gene="fnr"
                     /locus_tag="OO322_003986"
     CDS             complement(1401..2153)
                     /gene="fnr"
                     /locus_tag="OO322_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5908640.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2348..2863)
                     /gene="ogt"
                     /locus_tag="OO322_003987"
     CDS             complement(2348..2863)
                     /gene="ogt"
                     /locus_tag="OO322_003987"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5908641.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2874..3017)
                     /locus_tag="OO322_003988"
                     /pseudo
     CDS             complement(2874..3017)
                     /locus_tag="OO322_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3407..4222)
                     /locus_tag="OO322_003989"
     CDS             complement(3407..4222)
                     /locus_tag="OO322_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5908642.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4319..>4946
                     /locus_tag="OO322_003990"
     CDS             4319..>4946
                     /locus_tag="OO322_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5908643.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLE"
BASE COUNT         1306 a         1140 c         1182 g         1318 t
ORIGIN      
        1 gcgaaactga agtcagcgaa agggaagaaa tttctctttg gtttgttggc ggttttcatt
       61 attgcggcgt cggttgtgac tcgcgcgacc atcggcggcg ttatagaaca gtacaatatt
      121 ccgctgtctg agtggacgac atcaatgtat gtgatttagt catcgatgat ttttgtttat
      181 agcctggtct ttactgtgtt gctggcaatc ccgttgggaa tttatttcct tggcggcgaa
      241 gagcagtaag taaaaaatag gcccgataac tcgggccttg tcagttattg aagagtcgtt
      301 aatcgtcttc ttcgtcatcc agttcaacgg gtgtctgata ctggtcaggt ttaatgacca
      361 gcaggtcgca gcgaagatga tcaatcacct gttccgccgt gttgccgagg aatgctgctg
      421 aaataccggt gcgtccgacc gtgcccagaa ccacaattcc cgcctgtaaa tgctccgcca
      481 aatcaggaat cacctcttcg ggcagacctt tttctacgtg cgtcatgttt tcattaatgc
      541 cgaatttctg ccgcagggct ttcattgcca gcaaatgttg cccacgaatg gcatcgttat
      601 aaacgctcgg gtcaaattcc ggcagttcaa tcgcgatatt aattggcgtt accggataag
      661 cgccaaccag atgaacttcg gtatggttga cttgttctgc cagttcgatc gtctctttga
      721 ccagtttttc attgagcgca ttatgatacg gctcttcact ggcgagattc accgccacca
      781 gcgccttgcc tccttccggc cacggctggt ctttcaccat ccacaccggg cttgggcatt
      841 tgcgtaacag atgccagtcc gttggcgtaa aaatcaccgc ttccagacgg tcatgttggt
      901 gcgccatttt tagcaccaaa tcgtgtccgc cgctgatcac ttcctgaatg atggcttcga
      961 aaggacggtt atgccagacc actttaattt caatgggaac gccagcattg agataatatt
     1021 ttgcctgctc gtggatccag gctgtacgct ggctgatgac gccctgacgc atagcggtac
     1081 gttcgtccgg ggagagcagg gtggtcattt cgtatgagaa gtcatagatc ggcaaaaagg
     1141 ctttaatttt gccaccaatc cgttgatgta aataaacagc tcgccgcaat gctggttggt
     1201 cgtcctggtt aggatcgata acaacgagca tgttctgata catagccata cagggtctcc
     1261 ttacaacaac tgtcaacgca gtttgtaatt aaaagattaa cccatatctg gtgaatgaaa
     1321 cagtgatgaa ccttctgcca gatcaataaa tcagaaaaat ttaatgatat gacagaagga
     1381 tagtgagtta tgcggaaaaa tcaggcaacg ttacgcgtat ggccagcaag ctgggccagc
     1441 gcatcgttat tttcgatggt gatgtattta cctttgactg ccagcatacc gcttttctgg
     1501 aagcgaccca gcagacggct gatggtttct accgtcaggc ccagatagtt accaatatcg
     1561 ccacgagtca tcgtcaggcg gaattcacga ggggagaagc cgcgttgggc aaaacgacgg
     1621 gacaggttgt agatgaatgc agccagacgt tcctcggcat ttttcttcga caacagcaag
     1681 atcatgtcct gatcgccttt gatttcaccg ctcatcagac gcatcatctg ctgacgcaga
     1741 ttcggcattt taccggacaa atcgtccagc gtttcgaacg gaatttcaca taccatcgag
     1801 gtttccagcg cctgagcgaa gctcgggtga tggccgctgc cgatggcgtc aaatcccact
     1861 aggtcgcctg ctaaatggaa accagtgatt tgctcgtcgc cttgctcagt gattgtataa
     1921 cttttaatcg taccggagcg gatggcataa agcgatttaa gttcatcacc agccttaaac
     1981 agcgtctggc ctttctgaat aggcttcttc cgctcaatga tattatcaag ctgatcaagc
     2041 tcatgttcgt tgagtgtgaa cgggatgcaa agctggctga tgctgcaatc ctggcaatgg
     2101 atagcacaac cgccagactg aatgcgccgt ataattcgct tttccgggat cataggtctg
     2161 ctcaagccgt aattgatatt tgtcaatttt aacatctttt tagggagcaa gtaagtctaa
     2221 gcaaacctta acagcagaga attccgatat tagatgtaaa tatatgtcta tctatttgaa
     2281 actccttaag ttgtcaaggg taactttaca taaaagtgtg aacaagctgg cacaaattgt
     2341 ttaatgttta cagcaaaaga taaccttcgt ggcgcaataa ccactctttt cgctgaactc
     2401 cgcctgcata tccggtcatg gtgccgtttc ggccaataac ccgatggcaa ggtacgacga
     2461 tgctgatggg attcgatccg tttgccgcac caacggcacg cgccgcgcca gggcggccca
     2521 attgttcagc cagttggccg taatgcatta cctgcccgca ggggatagtg cgtagtgttt
     2581 tccagacttc gcgctgaaat ggcgtccccc ccgtggcagt gggaagcgta tcaataatgc
     2641 taagattacc ggcaaaatat tcacgaagct tgtcgcttaa accacctggg ttggtggcag
     2701 aaatgcgctc atagccttct ttgcgataat ggatgtccag cagctgcacc atgcgttcgc
     2761 tgtactcttc ccattcaacc gcccgcaggc gaaattgctc atcgcaaatc acccacagtg
     2821 gacccagtgg cgtggcaatt ttttcttcaa gtaatctcag catccgttct ctcttaagac
     2881 aaacgtgggt aaataccctg acctatcggc agaccgacaa gataccacgc cagcaacatc
     2941 agcagccata ccaccaaaaa gataagtgga tagggcaaga ctaacgaata gtaagtaccc
     3001 agtttcgcgt ctggtttgtc gcagtctgat agcataaagc gattttttaa tttttctgcc
     3061 tgaagaaatg agggcaagcc atcacatgga tcgagggtgg gataattcca gtcagtgaaa
     3121 ttttaccgta ggctatctgt tatttgattc tgtggcgtga tagccgccag acaccactga
     3181 actcgtcact cattccccaa tcccggcgag aatgtggtta tttacagagc taaaaaatga
     3241 ataaaaaaat agagcctgtc gacatccgcc agactctaca gtacacacag cagtgcatcc
     3301 tcgtcttaaa tccggcctgg ggtccaggtg accggtgaca gtgttataac ggttttaccc
     3361 atccgttgcc aattttcttc agtgacagat ttcacgaaaa tataagttaa atcatttgtc
     3421 ttgtgattgc ataattatgc atcgttaatc gacgagtaaa tttaccgggc gagtagcccg
     3481 ttcgacgctt aaacatattg ccgaacgatt cagctgaggc atagcccaga cgatgggcaa
     3541 ttttggtcaa cgaatatcca tcatcaagaa gcgtcactgc agaaataacg tgcatttgct
     3601 gaacccagtg gcgaaatgtt aatcccgtct ctttaacaaa taagcgcatg agtgtacggc
     3661 tactgacgtt aattttatcc gctagattat ccagtgttgg cagataccct tggttgttta
     3721 gcaaattctc acaaagctgc tgtagccgct catcatttgg ccaagggagt tcaagtgaca
     3781 ttttcacccc catttttatt tcctgaagta tcagtgtctc gagggctgaa aaacgtaact
     3841 gttgtttatc tgtggtactt tgcgacggtg gaagttggtt aattgcaatt agtaattccc
     3901 ttaacagggg tgaaataccc gtcatgaagc aaactgttcc cagcgtttca ctttccgctt
     3961 ctgaaaccag tacggttttt aaaagtacgt tgcctgtcgc aacaaactta tgcggcattt
     4021 gtgggggcag ccaaagcgcg ctgctggcat ggactagcca gacgctttct ccagcccata
     4081 cacgaacgac tccttcagcc gggtaaagca actgggcata gagatgttta tgccagggaa
     4141 ctgtacctcc attcagatgt tccgaatcca gtccgataat gtgatgccct ggtgatgctt
     4201 tgggttgcag tgtaattgtc atttacagta ggtattttgt cagaaatcat caaataagcc
     4261 atgttaaatt ttaaatatga cgtatgcaag tttccggata tatacggtgt aaagagtgat
     4321 gataatttct aaaaaacagc ttattggggt ggtagcgatc ggtattcttt tgactggcgt
     4381 tgtctttttt atctggaggg taagcaaagg aagatttata cagacgacgg atgatgctta
     4441 cattggtggt aatatcacca cggtggcaag taaagtttct ggttatattt ctgctataga
     4501 ggtgcgcgat aatcagagcg taaaaaaagg cgatattatc ctgcgtctcg atgatcggga
     4561 ttatcgtgca aatgtcgcca ggcttgaggc aaaaataaaa agtagcaaag ccaatctcga
     4621 aggcattcaa gccactatta cgatgcagca gtcgataatt caaagtgcca gtgaaacatg
     4681 gcaggcagta aaacatgagg agcaaaagcg cctgcgagat actgaacgtt atgaaaaact
     4741 cgcacaatca gcggcaatat cgcaacaaat tattgataac gccaggtttg actaccagca
     4801 ggtcgcagca aaggagcgca aagccgcgaa cgatttcctg gtggagaagc aacggctggc
     4861 agttttatct gctcaggaag aaaacgtacg tgcgagtatc gaagaagttc aggccgcttt
     4921 gactcaggca ctactggatc tggaat
//
LOCUS       DAJTRM010000182         4802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRM010000182 DAJTRM010000000
VERSION     DAJTRM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.185"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..799
                     /gene="tauA"
                     /locus_tag="OO322_003991"
     CDS             <1..799
                     /gene="tauA"
                     /locus_tag="OO322_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5908644.1"
                     /translation="RKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLA
                     SKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQ
                     PPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAE
                     KHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYL
                     TPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            812..1579
                     /gene="tauB"
                     /locus_tag="OO322_003992"
     CDS             812..1579
                     /gene="tauB"
                     /locus_tag="OO322_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5908645.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1576..2403
                     /gene="tauC"
                     /locus_tag="OO322_003993"
     CDS             1576..2403
                     /gene="tauC"
                     /locus_tag="OO322_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5908646.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2400..3251
                     /gene="tauD"
                     /locus_tag="OO322_003994"
     CDS             2400..3251
                     /gene="tauD"
                     /locus_tag="OO322_003994"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5908647.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3357..4331)
                     /gene="hemB"
                     /locus_tag="OO322_003995"
     CDS             complement(3357..4331)
                     /gene="hemB"
                     /locus_tag="OO322_003995"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5908648.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1088 a         1206 c         1364 g         1144 t
ORIGIN      
        1 gcgtaagttt gacagcggag ccagcatcgt gcgggcgctg gcttctggcg acgtgcaaat
       61 cggcaacctc ggttccagcc cattagcggt tgcagccagc caacaggtgc cgattgaagt
      121 cttcttgctg gcgtcaaaac tgggtaactc cgaagcgctg gtggtaaaga aaactatcag
      181 caaacccgaa gatttgattg gtaaacgcat tgccgtaccg tttatctcca ccacccacta
      241 cagcctgctg gcggcgctga aacactgggg gattaaacca gggctagtgg agattgtgaa
      301 cctgcagccg cccgcgatta tcgccgcatg gcagcggggt gatattgatg gtgcttatgt
      361 ctgggcaccg gcggttaacg ccctggaaaa agacggcaag gtgctgaccg attctgaaca
      421 ggtcgggcag tggggtgcgc caacgctgga cgtctgggtg gtgcgcaaag attttgccga
      481 gaaacatcct gaggtcgtga aagcgttcgc caaaagcgcc atcgatgcac agcaaccgta
      541 cattgctaac cctgacgcgt ggctgaaaca gccggaaaac atcagcaaac tggcgcgttt
      601 aagcggcgtg cctgaaggtg acgttccggg gctggtgaag ggaaatacct atctgacgcc
      661 gcagcaacaa acggcagaac tgaccggacc ggtgaacaaa gcaatcatcg acaccgcgca
      721 gtttttgaaa gagcagggca aggtcccggc tgtagcgaat gattacagcc agtacgttac
      781 ctcgcgcttc gtgcaataaa aggaggcgca gatgctgcaa atctctcatc tctatgccga
      841 ttatggcggc aaaccggcac tggaagatat caacctgacg ctggaaagcg gcgagctact
      901 ggtggtgctg gggccgtccg gctgcggtaa aaccaccctg ctgaatctga ttgccggttt
      961 tgtgccttat cagcatggca gcattcaact ggcgggtaag cgtattgagg gaccgggagc
     1021 agagcgtggc gtagtttttc agaatgaagg gctgctaccg tggcgcaatg tacaggacaa
     1081 cgtggcgttc ggcctgcaat tggcaggtat agagaaaatg cagcgactgg aaatcgcgca
     1141 ccagatgctg aaaaaagtgg ggctggaagg cgcagaaaaa cgctatatct ggcagctttc
     1201 cgatggtcaa cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc cccagctgtt
     1261 attactcgac gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga tgcaaactct
     1321 gctgctgaaa ctctggcagg agacgggcaa gcaggtgctg ttgattaccc acgatataga
     1381 agaagcggtg tttatggcga ctgaactggt tctgctttca tccggccctg gccgtgtgct
     1441 ggagcggctg cggctcaact ttgctcgccg ctttgttgcg ggagagtcga gccgcagcat
     1501 caagtccgat ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg tatttgagca
     1561 acgggaggcg ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg gcggctgaaa
     1621 tggcgctggc cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc ggttttactc
     1681 actgtatggt ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt gccgccgccg
     1741 caacaagtac tggcaaaact gctcaccatt gccggaccgc agggatttat ggacgccacg
     1801 ctgtggcagc atctggcagc cagcctgacg cgcattgtgc tggcgctact cgcggcggtg
     1861 gtgatcggta ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg cggcattctg
     1921 gatccggtaa tcgaacttta tcgtccggtg ccaccgctgg cttatttgcc gctgatggtg
     1981 atctggtttg gtattggtga aacctcgaag atcttactga tctatttagc gatttttgcg
     2041 ccggtggcga tgtcggcgct ggcaggggtg aaaagcgcgc agcaggttcg cattcgtgcc
     2101 gcccagtcgc tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt gcccggcgcg
     2161 ctgccggaaa tcctcaccgg attacgcatt ggtctgggtg tgggctggtc tacgctggta
     2221 gcggcggagc tgattgccgc gacacgcggt ttaggattta tggttcagtc agcgggtgaa
     2281 tttctcgcga ctgacgtggt actggcgggg atcgcggtga ttgcgattat cgcctttctt
     2341 ttagaactgg gtctgcgcgc gttacagcgc cgtctgacgc cctggcatgg agaagtacaa
     2401 tgagtgaacg tctgagcatt accccgctgg ggccgtatat cggcgcacaa atttcgggtg
     2461 ccgacctgac gcgcccgtta agcgataatc agtttgaaca gctttaccat gcggtgctgc
     2521 gccatcaggt ggtatttctg cgcgatcaag ctattacgcc gcagcagcaa cgcgcgctgg
     2581 cccagcgttt tggcgaactg catattcacc ctgtttaccc tcatgccgaa ggggttgacg
     2641 agatcatcgt gctggatacc cataacgata atccgccgga taacgacaac tggcataccg
     2701 atgtgacatt tattgaaacg ccacccgcag gggcgattct ggcagctaaa gagttacctt
     2761 cgaccggcgg tgatacgctc tggaccagcg gtattgcggc ctatgaggcg ctctctgttc
     2821 ccttccgcca gctgctgagc tggctgcgtg cggagcatga tttccgtaaa tcattcccgg
     2881 aatacaaata ccgcaaaacc gaggaggaac atcaacgctg gcgcgaggcg gtcgcgaaaa
     2941 atcctccgtt gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa caggcgctgt
     3001 ttgtgaatga aggctttact acgcgaattg tcgatgtcag cgagaaagag agcgaagcct
     3061 tgttaggctt tttattcgcc catatcacta aaccggagtt tcaggtgcgt tggcgctggc
     3121 aaccgaatga tattgcgatt tgggataacc gcgtgactca gcactatgcc aatgccgatt
     3181 acctgccaca gcggcggatt atgcatcggg cgacgatcct tggggataaa ccgttttatc
     3241 gggcggggtg atacgagtgc ggacggttcc ctcgccccct tggggagagg gttagggtga
     3301 gggggcgttc actgtacttt caacaggtta actccccctt tctgagagga aaaaaattaa
     3361 cgcagaatct tcttctcagc caaatccagc gcaaagtagc tgaaaatcag atccgcaccc
     3421 gcacgcttaa ttgaacctaa gctttcgagc acgactttct cttcatctat agcacccgcc
     3481 agcgcggcga acttaatcat cgcgtactca ccgctcacct gatacgcgcc aatcggcaat
     3541 tcagtacgtt cacgcagctc acgcacgatg tcgaggtacg ctccagcagg tttaaccatc
     3601 aggcagtctg cgccctgggc ttcatccagc agtgactcac gaatcgcctc gcgacggttc
     3661 attgggttca tctgatagct tttgcggtcg ccttttaatg cgcttcctgc agcttcacgg
     3721 aacgggccat aaaaggaaga ggcgaacttg gtcgaatacg acataatcgc cgtatcttta
     3781 aagcctgcag cgtccagcgc ctgacgaatc gcctgtacct ggccgtccat cgcggcggaa
     3841 ggggcgatga agtctgcgcc tgcagcagct gcaaccacgg cttgcttgcc taaattttcc
     3901 agagtcgcgt cgttgtcgac gccatgctcg cacagcacac cgcagtgacc gtgagaagtg
     3961 tattcacaga aacaagtgtc tgacatgacg atcatttctg gcacagtctg cttgcagatg
     4021 cgcgacatac gcgccaccag tccatcttcc cgccaggcgt cgctgccggt ttcatcggta
     4081 tggtgagaga tgccaaaagt cattacggaa cggataccgg cgttggcgat gcgttcaatt
     4141 tcgcgtgcca gatgtttctc tggaatgcgc atcacgcctg gcatggcttc aacggctttg
     4201 tagtcgtcaa tttcttcttc aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt
     4261 gtctcttcaa acatagcgcg cagcgcagga gatttgcgca ggcgacgagg gcgttggatt
     4321 aagtctgtca tggtctgcct gatgtttgtg gaattaaggt gcacagtata cctgaagcgg
     4381 ggcaggagtg ttttacgaaa gttgtcgcga tgttgacaag aagagaatga aagagagacc
     4441 aggacatctt tgtaatagaa atagcaataa tttcatattc actgaaaata tttttaatct
     4501 ttatttatag ctcttggtta ttattttttg gcgtttggtt gtgctgctat aaattgaata
     4561 attcaaactt tgttgtatat tgataagata tttatctggt ttgtattttt gatatttata
     4621 acgcgtatgc atcaaaaata acattatctg caacatactg aaatatcgtc cttttatcaa
     4681 tttgatgcgt gtttttctcc ataaattata tatgtccaca ttcggactta ggggaaagaa
     4741 taattgaacc attcgtctgt aacgcagcat aatcgttagc gcgaaacata atatgttttc
     4801 ta
//
LOCUS       DAJTRM010000183         4773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRM010000183 DAJTRM010000000
VERSION     DAJTRM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.186"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OO322_003996"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OO322_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5908649.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OO322_003997"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OO322_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5908650.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OO322_003998"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OO322_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5908651.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OO322_003999"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OO322_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5908652.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4773)
                     /locus_tag="OO322_004000"
     CDS             complement(4148..>4773)
                     /locus_tag="OO322_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5908653.1"
                     /translation="TEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKG
                     IVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFYHREY
                     GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACW
                     LLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1264 a         1168 c         1126 g         1215 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt atcgtcaggt
     4621 ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc agagctgcca
     4681 gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg ctctgtcctt
     4741 tttcactttc cattaatgca ttgaattctg ttt
//
LOCUS       DAJTRM010000184         4769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRM010000184 DAJTRM010000000
VERSION     DAJTRM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.187"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            188..372
                     /locus_tag="OO322_004001"
                     /pseudo
     CDS             188..372
                     /locus_tag="OO322_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            515..736
                     /locus_tag="OO322_004002"
                     /pseudo
     CDS             515..736
                     /locus_tag="OO322_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(757..819)
                     /locus_tag="OO322_004003"
                     /pseudo
     CDS             complement(757..819)
                     /locus_tag="OO322_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1269..2681
                     /gene="ospC1"
                     /locus_tag="OO322_004004"
     CDS             1269..2681
                     /gene="ospC1"
                     /locus_tag="OO322_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5908654.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2785..2934)
                     /locus_tag="OO322_004005"
                     /pseudo
     CDS             complement(2785..2934)
                     /locus_tag="OO322_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3010..4707
                     /gene="ospD3"
                     /locus_tag="OO322_004006"
     CDS             3010..4707
                     /gene="ospD3"
                     /locus_tag="OO322_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5908655.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1658 a          856 c          894 g         1361 t
ORIGIN      
        1 caaaaaaatc atcttcgtga agcttcacga catagcgggg aagtttgctt ctttgagagg
       61 cgggtttacg tttacgggat ttagctgaac gggccatata accacctgaa agacaatgac
      121 attgcctgtt tttataacgg taattgcaga ccatgacaaa ccgcagccgt caggctgcct
      181 actcgagtca caaggctctt tgtgtccgat atgccggcag cgcattaccc atgaaactgg
      241 ctggaacgta caccataagg tgaagaaagt tatgggggcg gtgaagaatt atctaacctt
      301 gtcttgttac atccaaattg ccatcgacag ttacacagtt gtgaagccgg ctctctcaaa
      361 aagggcttat aaaggcttga gcggtgtacc gggaaaccgg tatgcaccgt tcttagggga
      421 gggcagtcca gcaatgaact gtccttaccc gacaaacatc caaaacgaac ggaacgtgct
      481 ggaatccgca tataacccgt tgtaaagtga ggaaatggac acctcacttg ctcatgagaa
      541 cgcccgcctg cgggcactgt tgcagacgca acaggacact atccaccaga tggctgaata
      601 caaccgcctg ctctcacagc ggatggcggc ttatgcttcc gaaatcaacc gactgaaggc
      661 gctggttgcg aaactgcagc gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa
      721 aaccgaacgg cagattccat tttcacgggc gatctacgcc acacaggctc tgggttgcag
      781 tgactgctcc attaaggcga ttttgaactc ataagggaag taacaaaaaa actgcaaaag
      841 tacaataatt atatatggcc aaactaacct ctattaaaaa acatcagttt ttcacactta
      901 gttgagcttc atgaaaaaat catcgaggat ttcattgcag aatacacatt ccactaactg
      961 gaagcactcc ataagaggaa tcagagaaag tatcgttttt ttatagtaaa attcattgct
     1021 gttcaataaa attgatatag ttattaaatt atgaaaataa tttcacaaaa aagattaact
     1081 tgagttctta agcatcggat tttagatatt ttgaaatatc tggcatcaca taacatcaaa
     1141 agtatataat actcttatct aattacatgc agtatattgt atatatactc aaaaattgta
     1201 attcataccc tgctatatct tgtagggaaa gatattaatt aaaactgttt tcatataagg
     1261 ttcattttat gaatatatca gaaacactga actcagcaaa tacccaatgc aatatagatt
     1321 ctatggataa cagattacat acattgtttc caaaagtgac atcagtgcga aacgctgcac
     1381 aacaaactat gccagatgaa aaaaatttaa aagatagtgc aaatattatt aaaagtttct
     1441 ttaggaaaac tatagcagca cagagttata gtagaatgtt ctctcaaggc tctaacttta
     1501 aatctttaaa tatagcaatt gatgcaccat cagacgctaa agcctcattt aaggctattg
     1561 agcaccttga cagattatcg aagcattata tatctgaaat aagggaaaaa cttcatcctc
     1621 tttctgcaga ggaactcaat ttgctttcgc taattattaa ttctgattta atcttcagac
     1681 atcaaagtaa ttctgatttg tctgataaaa ttttaaacat taagtcattc aataaaattc
     1741 agtctgaagg aatatgcaca aaacgaaaca catacgctga tgatataaaa aaaatagcta
     1801 atcatgactt tgtgtttttt ggcgttgaaa tctctaacca tcagaaaaaa caccccctga
     1861 atacaaaaca tcacactgtt gattttggtg caaatgcgta tatcattgat catgactctc
     1921 catatggata tatgacatta accgatcact ttgataatgc tattccacct gttttttacc
     1981 atgagcacca atcattttta gataaatttt cagaggttaa taaagaagtt agtcgatacg
     2041 tacatggaag taaaggaatt atagatgtac caatattcaa tactaaagat atgaagttag
     2101 ggctcggatt atacctgatt gactttatta gaaaaagtga agaccaaagc ttcaaggagt
     2161 tttgctatgg aaaaaatctt gcccctgtgg atctggatag aatcataaac tttgtttttc
     2221 agccagagta ccatatacct aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta
     2281 gagaaatatc cttagaggag gctgttacag catctaatta cgaagaaatt aacaagcagg
     2341 tcactaacaa aaaaattgct ctccaggctc tttttctttc gattactaat caaaaagagg
     2401 atgtcgcctt atatatatta tctaattttg agataactag acaagatgtt atttccataa
     2461 agcatgagtt gtatgatatt gagtatctac ttagcgctca taattcaagc tgtaaagtac
     2521 ttgagtattt tatcaataag ggattggttg atgtaaacac aaagttcaaa aaaactaata
     2581 gtggggattg tatgttggat aacgcaataa aatatgagaa tgcagaaatg ataaaactat
     2641 tattgaaata tggtgcaaca tctgacaata aatatattta atcaaaattg aatatcgttt
     2701 agcaaaaggc agtatttgca caaaatatcg atatattgtt tattgtcagt atggctcaat
     2761 gtgataatag ttggaaagtt tgatgggttt cgccccgttg tagcggtagt cgaactggaa
     2821 ggtcttcagg cactgcttac agcgatagag cagcccccct gaactggaat ggccgttccg
     2881 gatacccccc tgagtttcag agtaacggtg acaaaccaca tcaatctttg ccatcaatca
     2941 tccaaagggc aaagagtaca acaacactaa gtctgcgtca caacccatca atgaaaggaa
     3001 tatatacata tgccatcagt aaatttaatc ccatcaagga aaatatgttt gcaaaatatg
     3061 ataaataaag acaacgtctc tgttgagaca atccagtctc tattgcactc aaaacaattg
     3121 ccatattttt ctgacaagag gagtttttta ttaaatctaa attgccaagt taccgatcac
     3181 tctggaagac ttattgtctg tcgacattta gcttcctact ggatagcaca gtttaacaaa
     3241 agtagtggtc acgtggatta tcatcacttt gcttttccgg atgaaattaa aaattatgtt
     3301 tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta ttatttattt tgttgaaaac
     3361 ggttcatggg gagatattat tttttatatt ttcaatgaaa tgatttttca ttccgaaaaa
     3421 agcagagcac tagaaataag tacatcaaat cacaatatgg cattaggctt gaagattaaa
     3481 gaaactaaaa atggggggga ttttgtcatt cagctttatg atcccaacca tacagcaact
     3541 catttacgag cagagtttaa caaatttaac ttagctaaaa taaaaaaact gactgtagat
     3601 aattttcttg atgaaaaaca tcagaaatgt tatggtctta tatccgacgg tatgtctata
     3661 tttgtggaca gacatactcc aacaagcatg tcctccataa tcagatggcc tgataattta
     3721 cttcacccca aagttattta tcacgcgatg cgtatgggat tgactgagct aatccaaaaa
     3781 gtaacaagag tcgtacaact atctgacctt tcagacaata cgttagaatt acttttggca
     3841 gccaaaaatg acgatggttt gtcaggattg cttttagctt tacaaaatgg gcattcagat
     3901 acaatcttag catacggaga actcttggaa acttctggac ttaaccttga taaaacggta
     3961 gaactactaa ctgcggaagg aatgggagga cgaatatcgg gtttatccca agcacttcaa
     4021 aatgggcatg cagaaactat caaaacatac ggaaggcttc tcaagaagag agcaataaat
     4081 atcgaataca ataagctgaa aaatttgctg accgcttatt attatgatga agtacacaga
     4141 cagatacccg gactaatgtt tgctcttcaa aatggacatg cagatgctat acgcgcatac
     4201 ggtgagctca ttcttagccc ccctctcctc aactcagagg atattgtaaa tttgctggcc
     4261 tcaaggagat atgacaatgt tcccggactt ctgttagcat tgaataatgg acaggctgat
     4321 gcaatcttag cttatggtga tatcttgaat gaggcaaaac ttaacttgga taaaaaagca
     4381 gagctgttag aagcgaaaga ttctaatggt ttatctggat tgtttgtagc cttgcataat
     4441 ggatgtgtag aaacaattat tgcttatggg aaaatacttc acactgcaga ccttactcca
     4501 catcaggcat caaaattact ggcagcagaa ggcccaaatg gggtatctgg attaattata
     4561 gcttttcaaa ataggaattt tgaggcaata aaaacttata tggaaataat aaaaaatgaa
     4621 aatattacac ctgaagaaat agcagaacac ttggacaaaa aaaatggaag tgattttcta
     4681 gaaattatga agaatataaa aagctgaata ttatctcgtg gtgatacact gaatttgagg
     4741 taaccggctc atttaaaccg tctggtctg
//
LOCUS       DAJTRM010000185         4728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRM010000185 DAJTRM010000000
VERSION     DAJTRM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.188"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..579)
                     /gene="kptA"
                     /locus_tag="OO322_004007"
     CDS             complement(<1..579)
                     /gene="kptA"
                     /locus_tag="OO322_004007"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5908656.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQK"
     gene            829..1512
                     /locus_tag="OO322_004008"
     CDS             829..1512
                     /locus_tag="OO322_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5908657.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1509..1970
                     /locus_tag="OO322_004009"
     CDS             1509..1970
                     /locus_tag="OO322_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5908658.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1983..3155
                     /gene="iadA"
                     /locus_tag="OO322_004010"
     CDS             1983..3155
                     /gene="iadA"
                     /locus_tag="OO322_004010"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5908659.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3220..4131
                     /gene="hypT"
                     /locus_tag="OO322_004011"
                     /gene_synonym="qseD"
     CDS             3220..4131
                     /gene="hypT"
                     /locus_tag="OO322_004011"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5908660.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4124..4516)
                     /gene="iraD"
                     /locus_tag="OO322_004012"
     CDS             complement(4124..4516)
                     /gene="iraD"
                     /locus_tag="OO322_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5908661.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4513..4596)
                     /gene="idlP"
                     /locus_tag="OO322_004013"
     CDS             complement(4513..4596)
                     /gene="idlP"
                     /locus_tag="OO322_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5908662.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1102 a         1246 c         1196 g         1184 t
ORIGIN      
        1 tttttgtacc ccagtcggca taaacgacca ctcaagaaac tcaacagcaa ccgttgatgt
       61 cagccagacc ccgttttccg cttgccagaa agggatgcct cgtttcgcca tttcctgtgc
      121 tttgacggtt aagacaaccg gcgatccgtg tcttgctccc actttgcgtg ccgttgcttc
      181 atcagcagag agatgcacgt aatgacgatc ccctgcaatc agcccctgtt tttttatctc
      241 gtcaaggaaa cggcttgccg taccgtggta gagaaactgt ggcggtgttt tttcggcaaa
      301 ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg cggatgcacc taccatcact
      361 ggaataacta aaacgctttt tatcgctggt tgcgaccacg gtatccagca atgcacgggt
      421 taatcgcttg cccgcttttt gggcacaaag tatgagctta ttgatatccg cccatccttc
      481 acggtccagt acgataccaa tcgcctccgg tttgtgtcgg aggacaaaac ttaaaaactt
      541 gctcgtatcc gccagctctt tttcgttgta ctttgccatc gcgttttcgt ccctggattt
      601 tctgttttta tttactaatg tcattgtcac tgggtttatg aattttctgc aactcaatcc
      661 tgcgcagaac cttttccaca cagcgtaatt tttcccctat gcaaaaaacg catagccaga
      721 aacatacttg cattttcccc atacccacat ggctgataag gtttttcagt tccaggaatt
      781 ttcctgactc aagcaacaca caaacacaaa aaaacaatgg ggattgttat gacacaacaa
      841 ggggatgctg tagccgggga gctggcgaca gaaaaagtgg gcattaaagg gtatctggct
      901 ttttttctca ccataatctt ttttcccggt gttttttccg gaacagacag ctggtggcgc
      961 gtttttgatt tttccgttct gaacggatcg tttgggcaac tgcctggtgc gaatggagcc
     1021 accacctcat ttcgcggggt gggtggcgcg ggggctaaag atggctttct ctttgcgctg
     1081 gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga tggtcttggc
     1141 ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt gggcattcca
     1201 gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc cgccggtatg
     1261 accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat tttcgccgca
     1321 tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggcgt cgcggtgttt
     1381 gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt taaatttgtc
     1441 ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga
     1501 gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc gatggcgcgc
     1561 gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca
     1621 tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc tgtgaacccg
     1681 taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc gcattaatga
     1741 gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca ttaaccggcc
     1801 acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg caaaatgtcg
     1861 gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc attacggtct
     1921 gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa caaggagtta
     1981 ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat ttgtatgcgc
     2041 cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc gccgttgcca
     2101 gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc gggcagatcc
     2161 tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc gaagcaggtc
     2221 ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc gtcacgtcag
     2281 tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg ctcgccaaga
     2341 cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct tatcatgtcc
     2401 cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat cgtgtgattg
     2461 gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat cacctggcta
     2521 atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt
     2581 tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg gaaaactgcg
     2641 atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg ttgtttgagc
     2701 aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc attgacgaac
     2761 cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg ctggcacgcg
     2821 tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa gggaatttaa
     2881 cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag
     2941 actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta gccggtttcc
     3001 ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta ctggtcatga
     3061 cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc aaagacggca
     3121 aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc cctaatggac
     3181 gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg tggtgcggtt
     3241 ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa atgccgcaat
     3301 ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt
     3361 gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc actgcaactc
     3421 tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca gttagaaagc
     3481 aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa gatagccgct
     3541 gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc gccgctcttt
     3601 acctgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa
     3661 agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt
     3721 cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg agaagcactt
     3781 tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc ctacatggtg
     3841 cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac cttttttgtc
     3901 tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat tgcctggctg
     3961 ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct gaatcgggac
     4021 gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat gaatccagtt
     4081 gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg acattctcaa
     4141 gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc ggttcatcgt
     4201 cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct tctttcaaat
     4261 taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc agctgacggc
     4321 aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg ggcatgcctg
     4381 aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg agatcgctgc
     4441 ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa
     4501 gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg cttgccctga
     4561 acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt gttcattaca
     4621 tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct
     4681 atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaa
//
LOCUS       DAJTRM010000186         4630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRM010000186 DAJTRM010000000
VERSION     DAJTRM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.189"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            119..4237
                     /gene="pic"
                     /locus_tag="OO322_004014"
     CDS             119..4237
                     /gene="pic"
                     /locus_tag="OO322_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5908663.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4388..>4630)
                     /locus_tag="OO322_004015"
     CDS             complement(4388..>4630)
                     /locus_tag="OO322_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR5908664.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1240 a         1050 c         1226 g         1114 t
ORIGIN      
        1 acaaaaacac attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca
       61 ttagcttctt cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt
      121 gaataaagtt tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga
      181 acttgcccgc agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg
      241 cattccagca atctgtctgt gttactctca gatatcccag gcgggtattg tccgttccga
      301 tattgcctat cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc
      361 caatgatatt ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc
      421 aatggccgat ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta
      481 tatcgtcagc gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa
      541 tacatattcc cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct
      601 gaataaactg gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa
      661 tgcttataaa tacactgaac gttacaccgc tttttatcgg gtgggtagtg gtacgcagta
      721 cactaaggac aaggacggaa atttagttaa ggttgccggt ggatatgctt ttaaaacagg
      781 aggaaccaca ggagttcctc tgatatctga tgcaacaata gtctctaatc ccgggcaaac
      841 ttataatcct gtaaacggcc ctttacctga ctatggagcc cctggggata gtggttctcc
      901 tttgtttgct tatgataaac aacaaaaaaa atgggttatt gttgctgtat taagagcata
      961 tgcaggtatt aatggtgcta cgaactggtg gaatgtcata ccaacagatt atctgaacca
     1021 ggttatgcag gacgatttcg atgcccccgt agactttgtt tccggactgg gccccctgaa
     1081 ctggacatac gacaaaacat caggcacagg taccctgagc cagggcagta aaaactggac
     1141 catgcacggg cagaaagaca atgacctcaa tgccggtaaa aatctggtat tcagcgggca
     1201 gaatggtgca attatcctga aagacagtgt gactcagggt gccggttatc tcgaatttaa
     1261 agacagttac accgtatctg ctgaatccgg aaaaacatgg acgggtgccg gcattattac
     1321 tgacaagggg acgaatgtaa cctggaaggt caacggcgtt gccggtgaca acttgcataa
     1381 gctgggggaa ggaaccctga ccataaacgg aacaggtgta aacccgggag gactgaaaac
     1441 gggagacggt atcgttgtac ttaaccagca ggcagacact gcaggtaata tccaggcctt
     1501 cagttcagtg aacctcgcca gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt
     1561 caatccggat aacatttcat ggggataccg gggaggtaag cttgacctta atggtaatgc
     1621 cgttaccttc acccgactgc aggctgctga ttacggggcg gtgattacaa ataatgcaca
     1681 gcaaaaatcc cagcttttac tggatcttaa ggctcaggat acaaatgtca gtgaaccgac
     1741 gattggaaat atatccccct ttggtggtac cggcactcca ggaaacctgt acagcatgat
     1801 actcaacagc cagacccgct tctatattct gaaatctgcc agctatggta acactctgtg
     1861 ggggaacagc ctgaatgatc cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc
     1921 agttcagaca gtaaaagata ggatcctggc cgggcgggca aaacaacccg ttatctttca
     1981 tggtcagctg accgggaata tggatgtcgc cattccacag gtgccggggg gaagaaaggt
     2041 aatctttgat ggtagcgtga acctgccgga aggtaccctg agtcaggaca gtggcaccct
     2101 gatattccag ggacatccgg ttatccatgc ctccatcagt ggcagtgcac cggtcagcct
     2161 gaaccagaaa gactgggaaa accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc
     2221 tgacttccat ctttcacgta acgcctcgct gaacagtgac attaagtcgg ataacagcca
     2281 tatcacactg ggaagtgaca gggcatttgt ggataaaaat gacggaacag gaaattatgt
     2341 cattccggag gaaggtacct ctgtcccgga caccgtgaat gacaggagcc agtatgaagg
     2401 gaatattacg ctgaaccata actcagccct ggatatcggc agcaggttca ccggggggat
     2461 tgacgcttat gacagtgccg tcagcatcac ctctccggac gtcctgttga cagccccggg
     2521 tgcttttgcc ggcagttcac tgacagtgca tgatggcggt catcttacag cactgaacgg
     2581 tcttttcagc gacgggcata ttcaggccgg taagaacggc aaaatcaccc tgagcggtac
     2641 accggttaaa gatacggcta atcagtatgc ccctgctgta tatctgacgg acggatatga
     2701 cctgaccggc gataacgcag cactggaaat tacccgtgga gcacatgctt ccggtgatat
     2761 tcatgcctct gcggcatcaa cagttaccat cgggtctgac acgccggcag aactggcttc
     2821 tgcggaaacg gctgcatcgg cgtttgccgg cagtcttctt gagggctata acgcagcatt
     2881 caatggtgcc ataaccggtg gcagggctga tgtcagtatg cataatgcac tgtggactct
     2941 gggtggggac tctgccatcc acagtcttac cgtcagaaac agccgtatta gttctgaagg
     3001 agaccgtaca ttccgtaccc tgacggtgaa taaactggat gcaacaggca gtgattttgt
     3061 tttgcgtacg gacctgaaaa atgccgataa aattaatgtg actgaaaaag ccactggttc
     3121 agataacagc ctgaacgtca gctttatgaa taatcctgct cagggacagg ccctgaatat
     3181 tcctctggtc acggcaccgg cgggaacttc agcagagatg tttaaggccg gcacccgggt
     3241 gacaggtttc agtcgggtga ccccaaccct gcatgttgat accagtggtg gcaatacgaa
     3301 gtggatactg gatggtttta aagcggaggc tgataaagcc gctgccgcga aggctgacag
     3361 tttcatgaat gccgggtata aaaacttcat gacggaagtt aacaatctga acaaacgtat
     3421 gggtgacctg cgtgacacaa acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc
     3481 cggttctgca gacggtggtt acagtgataa ttacacccat gttcaggtcg gctttgacaa
     3541 aaaacatgaa ctggacggtg tggacctgtt taccggtgtc acgatgacct ataccgacag
     3601 cagtgcagac agccatgcat tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc
     3661 ttcagcattg tttgagtccg gtgcctatat cgatttgatt ggtaaatata ttcaccatga
     3721 caatgattac acaggtaact ttgctagcct gggaacgaaa cactacaaca cccattcctg
     3781 gtatgccggt gctgaaacgg gttaccgcta tcacctgaca gaggacacgt tcattgagcc
     3841 gcaggctgaa ctggtttacg gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga
     3901 tatggacctg agcatgaaga acagggactt cagtccgctg gttggaagaa caggggttga
     3961 actgggcaag accttcagtg gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg
     4021 gcagtttgac ctgctgaata atggagagac cgtactgcgt gatgcgtccg gggagaaacg
     4081 gataaaagga gagaaggaca gccggatgct gtttaatgtt ggtatgaatg cgcagataaa
     4141 ggacaatatg cgctttggtc tggagtttga gaagtcagcc tttggtaaat ataacgtgga
     4201 taatgcggta aacgcgaatt tccggtatat gttctgatgt gggggtaaag ggctgccgga
     4261 aggcagcctt tctcagagac tcttaaaata taaaactgaa ggtcttcggt aagggggagt
     4321 gtattagacg tcaactagtt ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat
     4381 gcctttctca gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg
     4441 ccattcgcca gtccttcaac ggcatcgtcc atttctggga cgcagactgg attgccagcc
     4501 acaccacttt tttcaccgag tcgtctgtcg ggaacacttt acgctttttg atcgcatggc
     4561 ggatcacgct gtttagcgac tcgatggcat tcgtcgtata gatcactttg cggatgtccg
     4621 ttggataagc
//
LOCUS       DAJTRM010000187         4602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRM010000187 DAJTRM010000000
VERSION     DAJTRM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.190"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO322_004016"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO322_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5908665.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO322_004017"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO322_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5908666.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO322_004018"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO322_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5908667.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="OO322_004019"
     CDS             complement(3929..>4602)
                     /locus_tag="OO322_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5908668.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTRM010000188         4521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRM010000188 DAJTRM010000000
VERSION     DAJTRM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.191"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            124..1212
                     /locus_tag="OO322_004020"
     CDS             124..1212
                     /locus_tag="OO322_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5908669.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1212..1946
                     /locus_tag="OO322_004021"
     CDS             1212..1946
                     /locus_tag="OO322_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5908670.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2413..3174
                     /locus_tag="OO322_004022"
     CDS             2413..3174
                     /locus_tag="OO322_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5908671.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3218..4297
                     /locus_tag="OO322_004023"
     CDS             3218..4297
                     /locus_tag="OO322_004023"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5908672.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4294..>4521
                     /locus_tag="OO322_004024"
     CDS             4294..>4521
                     /locus_tag="OO322_004024"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5908673.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSIS"
BASE COUNT         1242 a          859 c         1114 g         1306 t
ORIGIN      
        1 attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg cgtagcgttg
       61 tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt gtcgttatac
      121 cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg tttattttgc
      181 gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc ccttagcggt
      241 ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat ctcaaactat
      301 gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga ccagcgtata
      361 cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg ttgggggtta
      421 ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac aaacatagag
      481 agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga aggtggcatc
      541 gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg tatatgccgc
      601 ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat tgccttaatg
      661 gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc tgttccccat
      721 ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat gaatcaggta
      781 cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat tatgaccgcc
      841 gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag agctgaagct
      901 gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata cctgacgcag
      961 gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg gttcgttcgc
     1021 gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc aatccacaat
     1081 gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga ttatctgaca
     1141 gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg ttttactcgc
     1201 gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca aacatcaact
     1261 gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt ggcctggatc
     1321 tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg aaacttcagg
     1381 ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc gtgatatggg
     1441 taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag gtttgtcgtc
     1501 gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga atgacgaatg
     1561 atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg gatattttat
     1621 tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg tttataaaac
     1681 gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt agcacacgta
     1741 aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag tccgcaattc
     1801 gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt aatgctcaac
     1861 ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa agatgtaaca
     1921 atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct ttttcgatga
     1981 cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt tttgccacgg
     2041 gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag agagttaata
     2101 aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg ttttttaaat
     2161 tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt gtaggctttt
     2221 gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata acaaaattaa
     2281 gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag taaggcgttg
     2341 taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt tgtcgaaatg
     2401 gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg cttgtcgttt
     2461 ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca aaacacttca
     2521 gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc ggatggcgca
     2581 gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa agtctttgaa
     2641 gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag gcttaatcca
     2701 ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga cagggaaaaa
     2761 ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg ggccgagaaa
     2821 gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt acatacctgc
     2881 ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc gagtgagaag
     2941 ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc gttctacatc
     3001 aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta tattgcgccg
     3061 ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa atggtctgtg
     3121 attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc ataatgtatt
     3181 acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac tgtcattaat
     3241 tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat ttgattcttc
     3301 acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg gagagcaact
     3361 ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg ttggggagtt
     3421 tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt taaccgttga
     3481 tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg acaataaaaa
     3541 gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg attttgatag
     3601 ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa gtagtttagc
     3661 accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc agacaaatat
     3721 tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt atggcaggtt
     3781 cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat ggagcaagga
     3841 acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag gcataaatgc
     3901 gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt ttgacagcat
     3961 accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg aggacagcta
     4021 ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta ttaaacaaaa
     4081 tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg ataatttacc
     4141 aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg gcgaggagca
     4201 gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat atttcaaata
     4261 tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt ctccgtgggc
     4321 gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg gattcaaaac
     4381 gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct gttagggagt
     4441 ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa atatcgtatt
     4501 cgttatagta aatccattag t
//
LOCUS       DAJTRM010000189         4377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRM010000189 DAJTRM010000000
VERSION     DAJTRM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.192"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..126)
                     /locus_tag="OO322_004025"
     CDS             complement(<1..126)
                     /locus_tag="OO322_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5908674.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEI"
     gene            complement(123..2762)
                     /locus_tag="OO322_004026"
     CDS             complement(123..2762)
                     /locus_tag="OO322_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5908675.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2970..3959
                     /gene="ldhA"
                     /locus_tag="OO322_004027"
     CDS             2970..3959
                     /gene="ldhA"
                     /locus_tag="OO322_004027"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5908676.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4070..>4377
                     /gene="hslJ"
                     /locus_tag="OO322_004028"
     CDS             4070..>4377
                     /gene="hslJ"
                     /locus_tag="OO322_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5908677.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1116 a         1137 c         1045 g         1079 t
ORIGIN      
        1 gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag gtgcagcgac
       61 ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag ccagtaaaat
      121 tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg cagacgtgcg
      181 ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca cagcgtaaaa
      241 atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc aacgcgactg
      301 gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt aatttttgtc
      361 gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc agtcatattg
      421 tctttcacca ccgcatctgc ggtatcttta tccagccgca aggtcattag cccgctattt
      481 gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg caatgcacca
      541 ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag ttcgctggat
      601 gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa ttgtttcatc
      661 agcacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg ttcttcttca
      721 gtccacggat aacgcccttg caaatcagcc gtaatatttt tcgctgtcac cagattaatc
      781 acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc tccatcggca
      841 aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat ccaggcacta
      901 ccgcctttca acacgccgtg ccctcccgcg cggaatcctt gttcaggcgc agcagaaaat
      961 gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg tggcaccagc
     1021 ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag acgaataccg
     1081 tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc gcctttaaag
     1141 aggaaatagg tcggattgcg cccatcgacg ctaaatttta aggttgatgg cggtaacaca
     1201 ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct gaaggaggga
     1261 tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg cttttctacc
     1321 gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag cgtaatcgtg
     1381 ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg tgtaaaactc
     1441 ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag ggagaaaatc attcgccagc
     1501 ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa aatggcctgc
     1561 aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca gcggatttca
     1621 tcgatgtcca gcgaatcgat gactcttccc tttgaacgaa gtaacgcgcc tggctcaaag
     1681 gtcagcgttg ggtcagacag acttccactt agctgcgcag gtaaacgggc atataaaatg
     1741 aggtccgcct gtttcgcttc accggtcagc tgcagaggca gttgactgtt atccatgctt
     1801 aattttcctg ggccaaaatt aagcaccgcg ttgcccttac ccgcttgccc ctgggtcagc
     1861 acattcagtc gtccgctgac cagagcgttc tcaagccctg cctgccagtt gtcgactttg
     1921 acacccagtc ggccactcaa aggaaaacca gcatacagcc agctccagcg accatcgctt
     1981 acggtcaatt gttgacgagt aatttgccac ggcaaatcga gcaacggatc gccgttatcc
     2041 cgtgccagca cgatcaattg cccgctattt tcctgccagt ccagctcggc atccaccagt
     2101 gacggttcct gcggcaagtt taacgtcgca gtagcatgcc cacttacagg aagtccatcc
     2161 ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt ttcaaacgcg
     2221 acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg aaatttaact
     2281 ttttcgccct gataacgcag ttgctggata tcagaggtta atgcgagaga gagttttccc
     2341 tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt gttaggcagc
     2401 atggactgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt ctgcggcaat
     2461 ttcgccagac aagcagaatc aagttctacc gtgccgacgt tcaataacca gcggctggga
     2521 tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag ataacggaga
     2581 tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc aatacgtgtc
     2641 ccgagcggta gccagatgtc cgccagcgtg ggaacccaca gcccgagcgt catcagcagc
     2701 gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata tttacccagc
     2761 atgggtagtt aatatcctga tttagcgaaa aattaagcat tcaatacggg tattgtggca
     2821 tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga tgaatttttc
     2881 aatatcgcca tggctttcaa ttatatttga aattttgtaa aatattttta gtagcttaaa
     2941 tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc acaaaacagt
     3001 acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg gaattttttg
     3061 actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg gtatgtattt
     3121 tcgtaaacga tgacggcagc cacccggtgc tggaagagct gaaaaagcac ggcgttaaat
     3181 atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgatgcggca aaagaactgg
     3241 ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa cacgccatcg
     3301 gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc cgtgacgcta
     3361 acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca ggcgttatcg
     3421 gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt atgcgtctgc
     3481 tggcgttcga tccgtatcca agtgcagcgg cgctggaact cggtgtggag tatgtcgatc
     3541 tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg acaccggaaa
     3601 actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg atgatcgtca
     3661 ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg aaaaatcaga
     3721 aaattggttc gttgggtatg gacgtctatg agaacgaacg cgatctgttc tttgaagata
     3781 aatccaacga cgtgatccag gatgacgtat tccgtcgcct gtctgcctgc cacaacgtgc
     3841 tgtttaccgg gcaccaggca ttcctgacag cagaagctct gaccagtatt tctcagacta
     3901 cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg cccgaacgaa ctggtttaat
     3961 cttgcagctc ccctgcaatc caggggagct gattcagata atccccaatg acctttcatt
     4021 ctctattctt aaaatagtcc tgagtcagaa actgtaattg agaaccacaa tgaagaaagt
     4081 agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg acaaaattgc
     4141 tgttacgcca gaacagctac agcatcatcg ctttgtgctg gaaagcgtaa acggtaagcc
     4201 cgtgaccagc gataaaaatc cgccagaaat cagctttggt gaaaaaatga tgatttccgg
     4261 cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac tgacagccaa
     4321 agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac tcgataa
//
LOCUS       DAJTRM010000190         4267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRM010000190 DAJTRM010000000
VERSION     DAJTRM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.193"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(104..709)
                     /gene="azoR"
                     /locus_tag="OO322_004029"
     CDS             complement(104..709)
                     /gene="azoR"
                     /locus_tag="OO322_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5908678.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            910..>4267
                     /gene="hrpA"
                     /locus_tag="OO322_004030"
     CDS             910..>4267
                     /gene="hrpA"
                     /locus_tag="OO322_004030"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5908679.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQ"
BASE COUNT         1059 a         1091 c         1163 g          954 t
ORIGIN      
        1 tgcttaagga agagcacaaa gcgatgttga aattatggga aaacaggtaa gtggattgag
       61 atgtggactg aatatctaca gtccacatca agaccgtgtc cggttatgca gcaacaatgc
      121 tgtcgatggc tgcttttgcg tcagactgtg ctttcgctgc catttccgga ccgtatgcga
      181 tcccttcggc gaagacaaat ttcacatcgg taatgccgat aaagccgagg aacgtggaca
      241 gatacggcgt caccaggtcc gttggtccat ctttgtggat cccaccgcgg ctggtaataa
      301 cgatggcttt tttacccgtt accagacctt ccggaccgtt ctcggtatag cggaaagtaa
      361 cgcctgcgcg tgcaaccagg tcaaaatagt ttttcaactg agttgagatg ttgaagttat
      421 acatcggtgc cgcaataacg ataacgtcgt gggctttcag ctcggcaatc agctcatcgg
      481 aaagcgccag agcttcctgc tgacgcggag tcagcggcgc atcgctcgga cgcagagcgc
      541 caaccagttc gccatccagt accggaatcg gatttgcagc taggtcgcga acggtgattt
      601 catcagcgga gtgcttttcg ctccattgtt caacaaaata atcggacaac tgattagact
      661 gagagtaccc tgccaggatg ctggatttaa gaactaatac cttgctcatg gtgtttcctt
      721 atagatgttt gaatggacga tgccccgttg cttgctgaca ctttattcac aatcctgcca
      781 cagagatcgc gcaataaatc gaagtctatg ttcgaattta ttgaacaacg catagaaagc
      841 cgcgatgtgg tactctatat ctatcattta aaagaaaatt aatcaggcag actactgccc
      901 actaacgtta tgacagaaca acaaaaattg acctttactg ccttgcaaca gcggctggat
      961 tcgctgatgc tgcgcgacag actgcgtttt tctcgccgtc tgcattgtgt taagaaggtt
     1021 aaaaatcctg atgcacaaca ggccattttc caggagatgg cgaaagagat tgaccaggcg
     1081 gcagggaaag tcctgctgcg tgaagcggca cgaccggaaa tcacttatcc tgacaattta
     1141 ccggttagtc agaaaaaaca ggacatactc gaagcgattc gtgatcacca ggtggtgatc
     1201 gtcgccgggg aaaccggctc cggtaaaacg actcagctac cgaaaatctg tatggagctg
     1261 gggcgcggta ttaaagggct gatcggtcat acccagccgc gtcgcctggc ggcacgaacg
     1321 gtggccaacc gtattgcgga agagctgaaa acggagccgg gcggttgcat cggttacaaa
     1381 gtgcgtttca gcgatcacgt aagtgataac acgatggtca agctgatgac cgacgggatc
     1441 ctgctggcgg agatccagca agaccgcctg ctgatgcagt acgacaccat cattattgac
     1501 gaagcgcacg aacgcagcct gaatatcgat tttctgctcg gctatttgaa agagttgctg
     1561 ccgcggcgtc ctgacctaaa aatcattatc acttccgcga ctatcgaccc ggaacgcttt
     1621 tcgcgccact ttaataatgc gccgattatt gaagtctccg gtcggaccta tccggtggaa
     1681 gtgcgctatc gcccgattgt tgaagaagcc gatgacaccg agcgcgatca gttgcaggcg
     1741 atttttgacg ccgtagacga actaagccag gaaagccccg gcgacattct gatctttatg
     1801 agcggcgagc gggaaattcg cgataccgcc gatgcgctga acaagctgaa cttacgccat
     1861 accgaaatct tgccgcttta tgcgcggctt tcgaacagcg aacagaaccg cgtgttccag
     1921 tcgcacagcg gacggcgcat tgtgctggcg accaacgtcg cggaaacctc gctgaccgta
     1981 ccggggatta aatacgtcat cgaccccggt acagcgcgta tcagccgcta cagctatcgc
     2041 accaaagtgc agcgtttgcc gattgaaccc atttcgcagg cgtctgccaa tcagcgtaaa
     2101 ggccgctgtg gtcgtgtgtc cgaagggatc tgtattcgtc tctattccga agacgatttc
     2161 ctctcgcgcc cggagtttac cgatccggag attctgcgta ccaacctggc ctcggttatt
     2221 ttgcagatga ccgcgctggg gctgggcgat atcgctgcgt tcccgtttgt cgaagcaccg
     2281 gataaacgca atatccagga tggcgtgcgt ctgctcgaag aactgggcgc gatcaccacg
     2341 gatgaacagg ccagcgccta taaactgacg ccgctcggtc gccagctctc gcagttgcct
     2401 gtcgacccac gtctggcgcg tatggtgctg gaagcgcaaa aacatggctg cgtgcgtgag
     2461 gcgatgatta tcacgtccgc gctctccatc caggatccgc gcgaacgtcc gatggacaaa
     2521 cagcaggcat cggacgaaaa acatcgtcgc ttccacgaca aagaatccga cttcctcgcg
     2581 tttgtaaatc tgtggaatta tcttggcgag cagcaaaagg cgctttcttc caacgccttc
     2641 cgtcgcctgt gtcgtaccga ttacctcaac tatctgcgcg tgcgcgaatg gcaggatatc
     2701 tacacccagt tgcgccaggt ggtgaaagaa cttggcattc cggtcaacag cgaaccggcg
     2761 gagtatcgtg aaattcacat cgccttactg accggtttgc tttcccatat cggcatgaaa
     2821 gatgccgata aacaggaata taccggtgca cgtaacgcgc gtttctccat cttccccggt
     2881 tctggtttat tcaaaaagcc gccgaaatgg gtaatggtgg cggaactggt agaaaccagc
     2941 cgcctgtggg ggcgcattgc tgcgcgtatc gacccggaat gggtagaacc cgttgctcag
     3001 catttgatta aacgcaccta cagcgaaccg cactgggaac gggcgcaggg cgcggtgatg
     3061 gcaacggaaa aagtcactgt ttatggtttg ccgattgttg ctgcgcgcaa ggtcaactac
     3121 agccagatcg attcggcgtt atgtcgtgaa ctctttattc gccacgctct ggtggaaggt
     3181 gactggcaga cgcgtcacgc attcttccgt gaaaacctga aactgcgggc cgaagtggaa
     3241 gagctggaac acaaatcacg tcgccgcgat attctggttg atgacgaaac gctgtttgag
     3301 ttctacgacc agcgcatcag ccacgatgta atctccgctc gtcacttcga tagctggtgg
     3361 aaaaaagtca gccgcgaaac gcctgatttg ctcaactttg aaaagagcat gttgatcaaa
     3421 gaaggggcgg aaaaaatcag caagctggat tacccgaact tctggcatca gggcaatctc
     3481 aagctgcgtt tgagatatca gtttgagcca ggcgcggacg cggacggtgt gaccgtacat
     3541 attccgctgc cgttacttaa ccaggttgag gaaagcgggt ttgaatggca gatccccggt
     3601 ctgcgccgcg aattggtgat tgctctgatt aaatcgttgc cgaaaccggt acgccgtaat
     3661 tttgtgcctg caccaaacta cgccgaagcg tttttaggcc gcgtcacacc gctggagtta
     3721 ccgttgctcg acagccttga gcgcgagtta cggcggatga ccggcgttac cgttgaccgc
     3781 gaagactggc actgggatca ggtgcccgat cacctgaaaa tcaccttccg cgtggtggat
     3841 gacaaaaaca agaagctaaa agaagggcgc tcgctacaag atctgaaaga tgcgctgaaa
     3901 ggcaaagtgc aggaaacgct gtccgcggtg gcggatgacg gtatcgagca gagcggctta
     3961 catatctgga gttttggtca gctgccggaa agttacgaac agaagcgtgg caactataaa
     4021 gtgaaggcgt ggccggcgct ggtggatgaa cgcgacagcg tgacgatcaa actgtttgat
     4081 aacccgctgg agcaaaaaca ggcaatgtgg aatggtcttc gccgtctact gctgctaaat
     4141 atttcctcgc cgatcaagta tttacatgag aagttaccaa acaaagccaa gctggggctg
     4201 tactttaacc cgtatggcaa agtgctggag ctgatcgacg actgtatctc ctgcggtgtg
     4261 gatcaac
//
LOCUS       DAJTRM010000191         4214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTRM010000191 DAJTRM010000000
VERSION     DAJTRM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.194"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..304
                     /locus_tag="OO322_004031"
     CDS             <1..304
                     /locus_tag="OO322_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5908680.1"
                     /translation="IPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGV
                     TLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            638..2917
                     /gene="ydeP"
                     /locus_tag="OO322_004032"
     CDS             638..2917
                     /gene="ydeP"
                     /locus_tag="OO322_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5908681.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3165..3368
                     /gene="safA"
                     /locus_tag="OO322_004033"
     CDS             3165..3368
                     /gene="safA"
                     /locus_tag="OO322_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5908682.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3436..4197
                     /gene="ydeO"
                     /locus_tag="OO322_004034"
     CDS             3436..4197
                     /gene="ydeO"
                     /locus_tag="OO322_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5908683.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1195 a          910 c          963 g         1146 t
ORIGIN      
        1 aatacccctt ggcgtatatt gctcaagcga acaaaagctc tcattttatc tttcgggtgc
       61 caccactgac agtagccgac aagtgtttgc taacacagca cctgatgcaa caaaagcatc
      121 gggagtgggt gtcactctaa tgcgtaatgg aaaaatactg gctacgggag aaaatgtctc
      181 cctggggaca gtaaataaaa gcaaagtacc tctgggttta agcgcaacct atggacaaac
      241 aggaaacaag gtttcagccg gaaccgtcca gtcagtcatt ggtgtcactt ttatctatga
      301 ataattgtga ttttgacctt agtctatgcg ctccattcac gaaaattgcg cggataatta
      361 ttttgtgaag gctattatcc tacacctgta agaaaatccg cgcacaattg aagccagagt
      421 cttatttttt atattggaaa atatgttaat ttaaacacta tgagagatga tgcttaactt
      481 atcgtattta atctattgtt taacgataat agattaaaaa tagaaagaga gtagtccctt
      541 actcttatat atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta
      601 caaatcctaa taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta
      661 ccagggtgct gcaggtggtt ggggtgctgt taaatccgta gcgaatgcag tacgtaagca
      721 gatggatata cgccatgatg ttattgccat gtttgacatg aataagccag agggctttga
      781 ctgtccgggt tgtgcatggc cagatcctaa gaacagtgcg tcattcgaca tttgtgaaaa
      841 cggcgcaaaa gcaatcgctt gggaagtcac ggataagcag gtaaatgcct ctttctttgc
      901 tgagaatacg gttcaatcat tacttacctg gggagatcac gagcttgagg ctgcggggcg
      961 tctcactcag cctttgaaat atgatgccgt cagcgactgt tacaagccat taagctggca
     1021 acaagctttc gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga
     1081 attctatact tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg
     1141 tgaatacggg agcaataact ttcccgactg ctccaacatg tgccatgaac cgacaagcgt
     1201 gggtttggca gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag actttgagaa
     1261 gtgcgattta gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac
     1321 ttcgttgcgc gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga
     1381 acgtggcctg gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga
     1441 gactcagttg gccagtgcct actacaacgt gcgcattggt ggcgatatgg cgttgctcaa
     1501 ggggatgatg cgcctgttaa ttgagcgcga tgatgctgca agtgccgcag gtcggccctc
     1561 attgcttgat gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga
     1621 cgttctcaat tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat
     1681 cgccgaactg gctgacgcat atgccgctgc cgaacgcacc attatctgtt acggaatggg
     1741 gatcactcag cacgaacatg gtacccagaa cgtgcagcaa ctggtcaatt tgctgttgat
     1801 gaaaggtaac attggcaagc ctggtgcggg tatctgccca ctacgtggtc actctaatgt
     1861 acagggcgac cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc tggctcgcct
     1921 gggtgagcgc tatggcttcc ccccacctca tgcacctgga catgctgcaa ttgccagcat
     1981 gcaagcaata tgtacggggc aggctcgagc attgatctgc atggggggca actttgcact
     2041 ggcaatgcca gatcgggaag cgagcgctgt accgttaacg caattagatt tggcggtaca
     2101 cgtagccact aagcttaacc gctctcatct gctgaccgca cggcatagct atattctgcc
     2161 ggttctgggg cgtagcgaga ttgatatgca aaaaagcggt gcgcaggcgg taaccgttga
     2221 ggattcaatg tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct
     2281 gaaatcagag tgtgcagtgg tcgcgggaat cgcgcaggca gcactacccc agagcgtggt
     2341 agcctgggag tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct
     2401 gccagagttc gccgactata accagcgcat ccgtcatccc ggtggttttc acctgataaa
     2461 tgcagctgct gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa
     2521 agggctgtta gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg
     2581 cagccacgat cagtacaaca cgacgattta tggtatggat gatcgctatc gaggggtatt
     2641 cggtcaacga gatgtggtct ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa
     2701 cggcgaaaga gttaatctta ttgcgcttac gccagacggt aagcgcagct cacgccgcat
     2761 ggatagatta aaagtggtca tttaccctat ggctgaccgc tcactggtga cctattttcc
     2821 agaatcgaat cacatgctaa cacttgataa ccacgatcca ttaagtggta ttcctggcta
     2881 taaaagtatt ccggttgaat tagaaccatc aaattaatgc ctcttctcat ttcttctgct
     2941 gtcatccgca cagcagaaga attcctcatt gactattatt gcgcaatttg ctcacatgga
     3001 ttaaattaaa ctacatacta taagatataa acttctgcct acagctgtaa gaaactccgc
     3061 tcagtactga agcaccagtc ctatttcttc ttttctccag cctgttatat taagcatact
     3121 gattaacgac ttttaacgtt atccgctaaa taaacatatt tgaaatgcat gcgaccacag
     3181 tgaaaaacaa aatcacgcaa agagataact ataaagaaat catgtctgta attgtggttg
     3241 tcttattact gacacttacg ttgatagcca ttttttcggc aattgatcag ctgagtattt
     3301 cagaaatggg tcgcattgca agagatctta cacatttcat tatcaatagt ttgcaagact
     3361 ggaaataaac agcaaattat aaatgtgaaa tgtttaaaag gtatcgataa aactttattg
     3421 ttttaaggag ataaaatgtc gctcgtttgt tctgttatat ttattcatca tgctttcaac
     3481 gctaacattt tagataaaga ttacgccttc tccgacggcg agatcctgat ggtagataac
     3541 gctgttcgta cacattttga accttatgag cggcatttta aagaaatcgg atttaatgaa
     3601 aataccatta aaaaatatct acaatgcact aacatccaga cagtgacggt gcctgttcct
     3661 gcgaagtttt tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat
     3721 ctcaactcgg aagaacgcaa tcatcataat ttttcagaac ttttgctttt ttcttgcctg
     3781 tctatttttg ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt
     3841 tccgggaaag tgagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat
     3901 atttgtgact gcctgtacat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa
     3961 acgacattct cacagattct attagatgcc agaatgcagc acgcaaaaaa tttgatacgc
     4021 gtagaaggtt cagtcaataa aattgccgaa caatgtggtt atgccagtac atcttatttt
     4081 atttatgcgt tccgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt
     4141 tgtcaaaggc acacgggtat gaatacgggc aacacgatga gtgctttagc tatttgatta
     4201 tttgctaacg agta
//
LOCUS       DAJTRM010000192         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRM010000192 DAJTRM010000000
VERSION     DAJTRM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.195"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO322_004035"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO322_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5908684.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO322_004036"
     tRNA            complement(324..399)
                     /locus_tag="OO322_004036"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO322_004037"
     tRNA            complement(406..480)
                     /locus_tag="OO322_004037"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO322_004038"
     tRNA            complement(597..681)
                     /locus_tag="OO322_004038"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO322_004039"
     tRNA            complement(690..765)
                     /locus_tag="OO322_004039"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO322_004040"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO322_004040"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5908685.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO322_004041"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO322_004041"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5908686.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO322_004042"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO322_004042"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5908687.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTRM010000193         4134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRM010000193 DAJTRM010000000
VERSION     DAJTRM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.197"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            18..686
                     /locus_tag="OO322_004043"
     CDS             18..686
                     /locus_tag="OO322_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908688.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(929..1624)
                     /locus_tag="OO322_004044"
     CDS             complement(929..1624)
                     /locus_tag="OO322_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5908689.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1617..3044)
                     /locus_tag="OO322_004045"
     CDS             complement(1617..3044)
                     /locus_tag="OO322_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5908690.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3055..3774)
                     /locus_tag="OO322_004046"
     CDS             complement(3055..3774)
                     /locus_tag="OO322_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5908691.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1098 a         1071 c          861 g         1104 t
ORIGIN      
        1 tattttctgg atatattatg agtgaacaaa tcaaacagga tatcgatctg attgagattt
       61 tatttcatct gaagaaaaag attcgtgtta tcctttttat tatggctatc tgtatggcaa
      121 tggtgctgtt gtttctgtat atcaataaag acaatacaaa agtgatttac agccttaaaa
      181 taaaccagac aacgccaggt atacttgtta gctgtgatag caataataat tttgcctgtc
      241 agaccacaat gactgaagat gttattcagc gaattactac attttttcag accagcccag
      301 atatcaagaa cagagaaata aggctggaat ggtcaggaga taagagagat ttaccaactg
      361 ctgaagagga aatatctcgc gtgcaggcgt ctattatcaa atggtatgcg tcagaatacc
      421 ataatggcag gcaagttctc gatgagatac aaacgccttc agcaattaac agtgagcttt
      481 atacaaaaat gatatacctg accaggaact ggtcattgta tccgaacggt gatggctgtg
      541 taactataag ctcaccagaa ataaaaaata aataccctgc tgccatttgc ctggctctgg
      601 gattttttct aagcattgta atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg
      661 aataccaaca aaactctggg cagtaataaa cagcattctg atagagagtg cctgtataaa
      721 gctgaagtga caatgaccgg gaagggaacc cgttttcagg aatggatgcc tgtctttttc
      781 actgtaaata tcctggcatt actaatgcct ggatatttac agaagagcag cataaccatg
      841 acggaacggg gttaagtcag gttgtcctta acgctatttt caacctcgta tgccttcttc
      901 aggtttatgt tcagatctca tatctctctc agcaatcctc aataatcaga tacaccgctt
      961 tcaccggacc atgaaccccg acgactttga taagctcaat atccgccgtt gagctgggac
     1021 cactgatgat gttaatacat gacggcattc gttcaccggc ctgcgctttc tgatgcaatt
     1081 tttctgcgag ttgcgctaca cgcggcagga ttgtgctttt acgcaggata aaaagagaag
     1141 attccgggag caggctcaat gaacgcccgc gctcggcggc ggaaaaaaga accacgcctc
     1201 ccgattcggt taaaccatat tcagcataca cgacacccac tttagcctgc tctgcctgcg
     1261 agatattctc ggcacctttc gccggatccc aaacaacggc attgcattcc tgctgcaaac
     1321 gttcactaat ccccaattcc tccagcctcg tgtcaccgct aatcacgacc gactgatctc
     1381 ccagctcttt acacagacgt attgcagctt ctgccgcctt cgcctcgctg gtcagctcac
     1441 agcgcgtcaa cataacatcg ctggcaaact gaataaacgc gtcacagcgc tgctgttggt
     1501 taagttgggt aagccgctcg ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt
     1561 caagtcgcag cgggcgaccc agtgcctgag caacgttatt caaaaattcg ctccgattat
     1621 ccattctttt tctcctgcgc ctgatgtttc ttaaaccaac tacggaaact ctctccgtca
     1681 gcttcaggaa gatcgcgtgc ttccatccag tcgctaatcg cgccaaattt gagtggtgtt
     1741 ttgccgccat tgataaacca gcttgccgca tgagcgccgg ccatcatccc gactttccac
     1801 aatcctggat gactattggc ataagcgaac atttttatcg cccgttgctc tgcttttgcg
     1861 gtgatccctt tttcagccat cacccgacga tgacgcaaaa tcagttttga cagcggaata
     1921 cgcaccggac acacgctgtc acaagcagtg cataaagagc aggcgtaggg taaatcttta
     1981 aaatctttat agccgccaag tagcggagaa atcaccgcac caattggccc tggataaata
     2041 gagccatatc catgaccgcc aatatggcga tatgccggac aagtattcat acaagccccg
     2101 cagcgaatac agcgcagcac atcccgaaat tcagaggcca gcacctcaga acgcccgtta
     2161 tcgacaataa ccagatgaaa ctcttcagga ccatcaacgt gccccgcttc gcgcggccct
     2221 gtcagccagg tgttgtatcc cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg
     2281 atcaatacat ctacctcggc aaacgtgggg gcaatacgct ccattcccat cactgcaata
     2341 tgcgttttag gcagcgtggt acacattcgc gcattacctt cattggtcac caggcatacc
     2401 gaaccggtct ctgccaccgc gaaattacag ccggtaatac ctatttcagc actgaggaaa
     2461 tcttcacgga ttttttgccg gatgaataag gtcatcgctt caggcgtttc cggcccctca
     2521 tagcccagac gttcgtgtag cactcgacgg atctgatggc gatctttatg aattgccggg
     2581 accacaacat gagatggcgg atcttgatcc agctggagaa tatattcacc cagatcggtt
     2641 tcaatcacct gaatgccagc atcctgcaac acatgattga caccaatctc ttcggtcacc
     2701 atcgatttag atttcaccac cttccgggca tttttgcgtt gggcaacctg taaaatgtag
     2761 cgggtagcgt cttctttggt ttttgcaaaa tagacgtgac cgccgttttg cgtcactttt
     2821 tctgagagct ggtacagata agcgtcgaga ttactcagaa catgatcacg tatctgggcg
     2881 gcccgatcgc gccactcctc ccagtgcccc aattcatcga ccattttttg ccgatttgcc
     2941 ccaatacgct gctgcgcgct tgccaccgct ttgcgcatga tcggatcttc aatttgctga
     3001 cggatgcgtg tcttaaaatc tgtattactg gttttgatcg acatctttat atcctcagcg
     3061 gctcatcaac acttcagcaa tatgcatcac tttgactttc tgcccttccc gttgtaatcg
     3121 cccactgatg ttcagcaggc aactcacgtc agcaccaatt aaatactcag ggcggacttc
     3181 catcaggtgc acaacctttt ctttcaccat ctcgccggat atttcggcca ttttgaccga
     3241 gaacgtgccg ccaaatccgc agcaggtatc ctgttcagca aaggtcaaca gctccagtcc
     3301 acgcacattt ttcagcagcg taagtggctc gtccttcact cccagcttac gggccaggct
     3361 acaagatggg tgatacacag ctctcccttg caaactggca cctacatcga ctacccctaa
     3421 tttattaaca ataaaagagg tgagatcctg catacgcgcg gcaacctttt cggcacgtga
     3481 tgcccattca ggttcatccg ccagatacgt cgggtaactt tttatggcat aggtgcaaga
     3541 gccagccggt gaaataatgg gatcgtcgtt atcctccagt gcggcgatca gatttttcat
     3601 ccctggaatc gcttctttga tataaccgct attgatcgca ggctgaccgc agcatccctg
     3661 tttctccggg aaatttacgc gacagccgag tttttccagt agcagcacgg agtctcgtgc
     3721 cattcttgat ttcagggcgt caccaataca ggtgacaaag aaattgacat tcacaactaa
     3781 tactccatta cttcatgccc atttatgccc gatgtttccc ccccaccggg ggatcaatca
     3841 tcaataaata tggcgttctt ttttaattgg ctagcaacat accacccctc atttgtatga
     3901 caaatgataa tctttcatcc aaacttcaaa tgcaataact aattttattt ctttcggcat
     3961 cgctcacatt ttatggggaa atcgacgatc atttttggca taagaaatcc atttaaaaat
     4021 ggctacatcc cttataccta ttcagagcta cttattcagg ggttattgag atatacactg
     4081 atggtgtaca aaaaaatcac cgacaataat cacgtatcaa gtaatagtcg gaat
//
LOCUS       DAJTRM010000194         4092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRM010000194 DAJTRM010000000
VERSION     DAJTRM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.198"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1568)
                     /locus_tag="OO322_004047"
     CDS             complement(<1..1568)
                     /locus_tag="OO322_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5908692.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLEL"
     gene            complement(1653..1988)
                     /locus_tag="OO322_004048"
     CDS             complement(1653..1988)
                     /locus_tag="OO322_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5908693.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(1988..2344)
                     /locus_tag="OO322_004049"
     CDS             complement(1988..2344)
                     /locus_tag="OO322_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5908694.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2344..3840)
                     /locus_tag="OO322_004050"
     CDS             complement(2344..3840)
                     /locus_tag="OO322_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5908695.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(3837..3998)
                     /locus_tag="OO322_004051"
     CDS             complement(3837..3998)
                     /locus_tag="OO322_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5908696.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
BASE COUNT          920 a         1246 c          920 g         1006 t
ORIGIN      
        1 agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt cagccctgtt
       61 tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat acttgccacc
      121 gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc ctgttcccat
      181 ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac aaccggaccg
      241 accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc aattaatccc
      301 tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt caatgtgcca
      361 atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt tacgacctca
      421 ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc ttttatgcct
      481 tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt gatctggttc
      541 tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc tttctgcatt
      601 gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa caaatccaga
      661 ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc tttcagcaca
      721 gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag catggccccg
      781 cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa cgagcggagc
      841 acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat aaaattcttt
      901 atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc ccccattgcg
      961 gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg ggtcacaaca
     1021 tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat cttatccgca
     1081 agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc catcatctga
     1141 ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc gtcagtaaac
     1201 tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat ttcggcgata
     1261 ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt cattgcctta
     1321 aacgcttccg gcgtatccag accatccacc actttccgga catccgccat ttttgattca
     1381 agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt tccggcaccc
     1441 gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg gcgctgcatt
     1501 cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag ctgaaaactg
     1561 tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt ctgtaaactg
     1621 cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg caacattgta
     1681 gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag gatctgcatg
     1741 ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg gatccccgcc
     1801 agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc ttcaccggaa
     1861 ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg caactccagc
     1921 acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc tgtaacgttc
     1981 attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc gtgccttctt
     2041 cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac accatgccgt
     2101 ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg accggaaagt
     2161 ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc ttgtaatcca
     2221 catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc acttcaatgc
     2281 cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acaggttccg gcgatctttg
     2341 ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg caaagacacg
     2401 taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt tatctgcatc
     2461 acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac cggcacgctc
     2521 catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga caatcgcctg
     2581 ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact tgcttgtgat
     2641 gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac tgtccagata
     2701 gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac gctggatctg
     2761 caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg tctgctgctc
     2821 tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct cccccgtctg
     2881 tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc gactggcaac
     2941 cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga tatgttgctg
     3001 attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg cggtatagac
     3061 atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca tttcggtcat
     3121 catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga atgcctcatc
     3181 gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag caccgacttc
     3241 cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat aataattcag
     3301 acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg tcaccacgcc
     3361 tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt ccttaatcgc
     3421 ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac gggagcgccc
     3481 gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca ccctgaccgt
     3541 cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga aaggatctgt
     3601 ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa tctggcgggc
     3661 ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat cgttgctggc
     3721 atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag aattgttcat
     3781 ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac ttatcgtcat
     3841 gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat acggcggaac
     3901 cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc acgggcaggg
     3961 tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta attctgaggg
     4021 aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg atcgatgtaa
     4081 tccacatcaa ta
//
LOCUS       DAJTRM010000195         4052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRM010000195 DAJTRM010000000
VERSION     DAJTRM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.199"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(896..1087)
                     /locus_tag="OO322_004052"
     CDS             complement(896..1087)
                     /locus_tag="OO322_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5908697.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1152..1475
                     /locus_tag="OO322_004053"
     CDS             1152..1475
                     /locus_tag="OO322_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5908698.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1417..2175
                     /locus_tag="OO322_004054"
                     /pseudo
     CDS             1417..2175
                     /locus_tag="OO322_004054"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2153..2683
                     /locus_tag="OO322_004055"
     CDS             2153..2683
                     /locus_tag="OO322_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5908699.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2690..2923
                     /locus_tag="OO322_004056"
     CDS             2690..2923
                     /locus_tag="OO322_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5908700.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3469..3861)
                     /locus_tag="OO322_004057"
     CDS             complement(3469..3861)
                     /locus_tag="OO322_004057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908701.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          969 a          922 c         1208 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctaacac ccgctgacaa gcccgtcaga
      901 ggttttcacc gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt
      961 cgtaaagcga ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca
     1021 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1081 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1141 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1201 gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg
     1261 cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
     1321 ggaataacct gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
     1381 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
     1441 ccgtcaggga acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt
     1501 ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg
     1561 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
     1621 tacacgtccg gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa
     1681 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt
     1741 ctgtgggtgg agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg
     1801 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt
     1861 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     1921 aaccgccgga cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag
     1981 agcattacac ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat
     2041 gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc
     2101 agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa
     2161 tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     2221 gaaaatgctg agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc
     2281 aaacgagacg aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact
     2341 gaaaaaacat cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa
     2401 gctggcaggg aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag
     2461 tggcgggacg ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa
     2521 gcagaacgag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg
     2581 gagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat
     2641 ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct
     2701 ggaaagccat ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa
     2761 cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa
     2821 cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca
     2881 gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga acggttgata
     2941 caggagcgcg aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg
     3001 acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg
     3061 tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc
     3121 agaccttgct gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc
     3181 ttttgtcgct gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt
     3241 tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg
     3301 tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa
     3361 tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     3421 tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt
     3481 gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga
     3541 ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa
     3601 ggcgtgagat tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct
     3661 tccgtccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac
     3721 ttcttgaatt tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt
     3781 aggggagtgt attattctct cttttaataa gctgtttacc acagggttta gttgttgtct
     3841 aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg
     3901 ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc
     3961 aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct
     4021 tttttgcggc gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTRM010000196         3868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRM010000196 DAJTRM010000000
VERSION     DAJTRM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.200"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(23..2056)
                     /gene="metG"
                     /locus_tag="OO322_004058"
     CDS             complement(23..2056)
                     /gene="metG"
                     /locus_tag="OO322_004058"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5908702.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2188..3297
                     /gene="apbC"
                     /locus_tag="OO322_004059"
     CDS             2188..3297
                     /gene="apbC"
                     /locus_tag="OO322_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5908703.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3559..3840
                     /locus_tag="OO322_004060"
     CDS             3559..3840
                     /locus_tag="OO322_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5908704.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          932 a          966 c         1008 g          962 t
ORIGIN      
        1 gatgcagcgc cttgaagggg gattatttca cctgatgacc cggtttagca ccggcatccg
       61 ggcttagcag gaaaatatct ttcccgccag gaccggcagc catcaccatg ccttcagaga
      121 taccgaagcg cattttacgt ggtgccaggt tagccaccat aatggtgtga cgaccaatca
      181 gtgcctgcgg atccgggtaa gcagaacgaa taccggagaa gacattgcgt ttttcaccgc
      241 cgagatccag cgtcaggcgc agcagtttgt cagaaccttc aacaaactct gcgttttcga
      301 tcagcgccac gcgcaggtca actttggcga agtcgtcaaa ggtgatggtt tcctgaatcg
      361 gatcatctgc cagtgggcca gtaactggcg cggtagtggc tttcacttct tctttagagg
      421 cttccaccag agcttcaacc tgtttcatat cgatgcggtt atacagcgcc ttgaacggat
      481 tcactttgtg gcccagcagc ggttgctgga taccatccca ggtcagttcc gtattgagga
      541 aggcttctgc acgctcggtc agtttcggca gtacaggctt caggtaagtc atcagtacgc
      601 ggaacaggtt gatgcccatt gagcaaatcg cctgcaggtc agcatcgcga ccttcctgtt
      661 ttgcgactac ccacggagcc tgctcgtcga catagcggtt agccaggtca gccagtgcca
      721 tgatttcgcg cactgcttta ccaaattcac ggctttccca cgcttcacca atcacttcag
      781 cggcatcggt aaaggttttg tacagctcag ggtcagccag ctcgcttgcc agcacaccgt
      841 caaaacgctt gttgataaag cccgcattac gggaggccag gttaaccact ttgttcacga
      901 tatcggcatt cacacgctga acgaaatctt ccaggttgag atcgatatca tcaatgcgcg
      961 aagagagttt cgcagtgtag tagtaacgca ggctgtcagc gtcaaaatga ttcagccagg
     1021 tgctggcttt aataaaggtg ccgcgagact tggacatctt cgcgccgttc accgtcacat
     1081 agccatgaac aaacaggttg gtcggcttgc ggaagttgct gccttccagc atggcaggcc
     1141 agaacaggct gtggaagtaa acgatatctt taccgatgaa gtggtacagc tcggcggtgg
     1201 agtctttctt ccagtattca tcgaagctta cgctgtcgcc gcgcttgtcg cacagattct
     1261 tgaaagaacc catgtagcca atcggtgcgt ccagccagac gtagaaatat ttgcccggtg
     1321 cgttcggaat ttcaaaacca aagtatggcg cgtcgcggga gatatcccac tgttgcaggc
     1381 cggattcaaa ccactcctgc attttgtttg ccacctgctc ctgcaacgca ccgctgcggg
     1441 tccatgcctg cagcatttcg ctgaaagagg gcagatcaaa gaagaagtgt tcagaatcac
     1501 gcatcactgg tgtagcacca gaaaccaccg atttcggctc gatcaattca gtcgggctgt
     1561 aggtcgcgcc gcagacttcg cagttatcgc cgtattgatc cggggattta catttcgggc
     1621 aggtgccttt cacaaaacgg tccggcagga acatgccttt ttccggatcg tacagctgag
     1681 agatggtgcg gtttttaata aaaccgtttt ctttcaggcg agagtagata agttctgaca
     1741 actggcggtt ctcttcgctg tgcgtcgagt gatagttgtc atagctgatg ttaaagcctg
     1801 cgaaatcagt ctgatgctcc tgactcattt cgccaatcat ctgctccggg gtgataccga
     1861 gctgctgagc tttcagcatg atcggtgtac cgtgggcatc gtcggcgcag atgaagttga
     1921 cctcgtggcc gcgcattcgc tggtaacgga cccagacatc agcctggatg tgctccagca
     1981 tatggccgag gtggattgag ccgttagcgt acggcagtgc gcacgtcacc agaattttct
     2041 tcgcgacttg agtcatagta ggcattactt cttaatagtg aaaaggggtt agatagtacc
     2101 aaatgggaaa atgttaagta agtacttctc tatattcttc aggtaaacta tcagctgatg
     2161 gtttatctat acaaaaaagg agtcgggatg aacgaacaat cccaggccaa atcaccagaa
     2221 gccttgcgcg caatggtcgc cgggacactg gccaattttc agcacccaac cctgaagcat
     2281 aacctcacca cgctgaaagc gttgcaccat gtcgcctgga tggacgacac gttgcatgtg
     2341 gaactggtta tgccgtttgt ctggcatagc gcgttcgaag agttaaaaga gcaatgtagc
     2401 gccgaactgc tgcgcatcac cggggcgaaa gctatcgact ggaaactgtc gcataacata
     2461 gccactctga aacgcgtgaa gaatcagccg ggaattaacg gtgtgaagaa cattatcgcg
     2521 gtgagttccg gtaaaggcgg cgtgggtaaa tcgtccacgg cggtaaacct ggcgctggca
     2581 ctggcggcgg aaggggcgaa agtcggcatt ctggacgccg atatctacgg cccatcaatt
     2641 cccaccatgc tgggcgcaga aaatcagcgt ccaacttcgc cggacggtac ccacatggca
     2701 ccgattatgt ctcatggcct ggcaaccaat tcaattggtt atctggtcac tgacgacaat
     2761 gcgatggtgt ggcgtggacc gatggcgggc aaggcgttaa tgcagatgtt gcaggaaacc
     2821 ttgtggccgg atctcgacta tctggtgctt gatatgccgc caggcaccgg tgacattcaa
     2881 ttgacgctgg cgcaaaacat tccagtaact ggtgcagtcg tggtcaccac cccgcaggac
     2941 atcgcgttga tcgatgcgaa aaaaggcatt gtgatgttcg agaaagtcga ggttccggtg
     3001 ctcggtattg tcgagaacat gagtgtgcat atttgcagta actgtggtca ccatgaaccg
     3061 attttcggta ccggtggtgc agagaaactg gctgaaaaat accatacaca gctgctgggg
     3121 cagatgccgt tacacatctc cttacgtgag gatctcgata aaggcacgcc aacggttatc
     3181 agccgtccgg agagcgaatt taccgctatc taccgccagc tggctgaccg cgttgcagct
     3241 cagctctact ggcagggtga agtcattcct ggcgagattt ccttccgcgc ggtctaacgc
     3301 ctattaacca ttctggttat tcttcataca ccgcagagat gttatattga tgcggtgtat
     3361 ttgtttttgc cgcattaatg aacacttatt taatactgcg aatattctgc ggaccttcgt
     3421 ccgaaactct acaaagtcga gtgatcgtac aggcaatgct tcattatcat ccttcgtgct
     3481 gtgtagtgga tgtacagcta tagtttgtta tgcactcatt ctatccaggc ataaccttta
     3541 ctacagggat aacgttcaat gaataagtat tggctgtcag gaattatttt tcttgcctat
     3601 ggattggctt ctcccgcagt ctcttcagag actgccacat taacgattaa tggcaggatc
     3661 agtcccccaa catgcagtat ggctatggtt aatagtcaac cacaacagca ttgtggtcag
     3721 ttaacgtata atgttgacac ccgtcatcag gtttcttcgc ccgtcaaagg tgttacgaca
     3781 gaagtggttg ttgcaggtag cgatagcaaa cgtcgaatcg tgttaaatcg ctacgattaa
     3841 tttggcaacc tgattttaat tgcattta
//
LOCUS       DAJTRM010000197         3855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRM010000197 DAJTRM010000000
VERSION     DAJTRM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.201"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1394
                     /gene="aldA"
                     /locus_tag="OO322_004061"
     CDS             <1..1394
                     /gene="aldA"
                     /locus_tag="OO322_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5908705.1"
                     /translation="TWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEW
                     EALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEW
                     ARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIK
                     PSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAG
                     EKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVY
                     VQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGAR
                     VALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSD
                     YGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGL
                     HEYLQTQVVYLQS"
     gene            complement(1436..2436)
                     /gene="gap"
                     /locus_tag="OO322_004062"
                     /pseudo
     CDS             complement(1436..2436)
                     /gene="gap"
                     /locus_tag="OO322_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2625..3155
                     /gene="cybB"
                     /locus_tag="OO322_004063"
     CDS             2625..3155
                     /gene="cybB"
                     /locus_tag="OO322_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5908706.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3400..3573
                     /locus_tag="OO322_004064"
     CDS             3400..3573
                     /locus_tag="OO322_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908707.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3645..3794)
                     /gene="hokB"
                     /locus_tag="OO322_004065"
     CDS             complement(3645..3794)
                     /gene="hokB"
                     /locus_tag="OO322_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5908708.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT          983 a          874 c          994 g         1004 t
ORIGIN      
        1 ttacctggcg tggagacgca tggattgatg tggtaaaccc tgctacagag gctgtcattt
       61 cccgcatacc cgatggtcag gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg
      121 cacaaccaga atgggaagcg ttgcctgcta ttgaacgcgc cagttggttg tgcaaaatct
      181 ccgccgggat ccgcgaacgc gccagtgaaa tcagtgcgct gattgttgaa gaagggggca
      241 agatccagca gctagctgaa gtcgaagtgg cttttactgc cgactatatc gattacatgg
      301 cggagtgggc acggcgttac gagggcgaga ttattcaaag cgatcgtccg ggagaaaata
      361 ttcttctgtt taaacgtgcg cttggtgtga ctaccggcat tctgccgtgg aactttccgt
      421 tcttcctcat tgcccgcaaa atggctcccg ctcttttgac cggtaatacc atcgtcatta
      481 aacccagtga atttacgcca aacaatgcga ttgcattcgc caaaatcgtc gatgaaatag
      541 gccttccgcg cggcgtgttt aaccttgtac tggggcgtgg tgaaaccgtt gggcaagaac
      601 tggcgggtaa cccaaaggtc gcaatggtca gtatgacagg cagcgtctct gcaggtgaga
      661 agatcatggc gactgcggcg aaaaacatca ccaaagtgtg tctggaattg ggtggtaaag
      721 caccggctat cgtaatggac gatgccgatc ttgaactggc agtcaaagcc atcgttgatt
      781 cacgcgtcat taatagtggg caagtgtgta actgtgcaga acgtgtttat gtacagaaag
      841 gcatttatga tcagttcgtc aatctgctgg gtgaagcgtt gcaggcggtt caatttggta
      901 accccgctga acgcaacgac attgcgatgg ggccgttgat taacgccgcg gcgctggaaa
      961 gggttgagca aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg ttgggtggca
     1021 aagcggtaga ggggaaagga tattattatc cgccgacatt gctgctggat gttcgccagg
     1081 aaatgtcgat tatgcatgag gaaacctttg gcccggtgct gccagttgtc gcatttgaca
     1141 cgctggaaga tgctatctca atggctaatg acagtgatta cggcctgacc tcatcaatct
     1201 atacccaaaa tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt
     1261 acatcaaccg tgaaaacttc gaagctatgc aaggtttcca cgccggatgg cgtaaatccg
     1321 gtattggcgg cgcagatggt aaacatggcc tgcatgaata tctgcagacc caggtggttt
     1381 atttacagtc ttaatgagtg aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga
     1441 gtttagcgaa tttttcgagg gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt
     1501 accaggcgac cgttttcacc agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg
     1561 catcaaacac tgaaccgaaa tggctgccaa tgatatcgga agagactatt tcttcatcgg
     1621 tataaccaaa tgactcgtta ttggtggttg cttgtttaag tgcgttattc acctcttcgg
     1681 cagtcacttt tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac
     1741 gcgttgcgca tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt
     1801 tgccgccccc gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc
     1861 tttaccacgc gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat
     1921 cgtgccgact tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt
     1981 agtggtgcat gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac
     2041 gttataaacg atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc
     2101 agcatcaaga tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat
     2161 gatttctgcc cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc
     2221 gatacttttc ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg
     2281 accatagttt gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt
     2341 aatagcgaca acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc
     2401 gatacgacca aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc
     2461 ggatgaaaat tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc
     2521 ttacagtgaa acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa
     2581 agcccgttat gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact
     2641 caaggttaca aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg
     2701 caatggagtt tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg
     2761 tttcctgtgg catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat
     2821 acccaacccc gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg
     2881 gacatttggt gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt
     2941 ataaccgggg caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca
     3001 atttcgaacg ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt
     3061 ttgtcatcgg gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca
     3121 cacttctacg catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta
     3181 ctaaaaccga cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata
     3241 tttatttgta tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt
     3301 aatcaattca cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt
     3361 tcaaatcgca cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt
     3421 ttgtttatgg gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt
     3481 tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct
     3541 attagtgcat cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat
     3601 gaatctgcta gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag
     3661 gccatgagcg cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt
     3721 cgggtcagga gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga
     3781 gggttgcgct tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa
     3841 ttgcttggtt cattc
//
LOCUS       DAJTRM010000198         3788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRM010000198 DAJTRM010000000
VERSION     DAJTRM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.202"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            419..2071
                     /locus_tag="OO322_004066"
     CDS             419..2071
                     /locus_tag="OO322_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5908709.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2082..2252)
                     /locus_tag="OO322_004067"
                     /pseudo
     CDS             complement(2082..2252)
                     /locus_tag="OO322_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2385..2504)
                     /locus_tag="OO322_004068"
                     /pseudo
     CDS             complement(2385..2504)
                     /locus_tag="OO322_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2578..3508)
                     /locus_tag="OO322_004069"
                     /pseudo
     CDS             complement(2578..3508)
                     /locus_tag="OO322_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1046 a          875 c          869 g          998 t
ORIGIN      
        1 aggctgggcc acatccctcc ggcagaagca gaaaaagctt attatgcttc tatcggaaac
       61 gatgatctgg cagcctgagt tcacagataa aacattctcc aggaaacttg gggcggttca
      121 cgtcagtatc agaccatatt aattgctgaa actgataaac atcaaacctt atagaagggt
      181 gaatgctgaa agggggctat ggtgatcgac gtgatgacaa cgacaggtaa gaccgtgacc
      241 tgataaacct gaattgtgtc ctagacataa agtccgtgaa gggaataagg ctcaggtcag
      301 cgaatcttat agtggcccgc agctctgact gcaataaggc cggatacaga gcagcaacta
      361 ctcgtcgatg tcagaacaac ggaagccttg caaacgggga tgcgttcata caggaacaat
      421 gataccgtta aaaaaaaaca ttacgctaat aatgttcacc ctgtcattac tgacaggaaa
      481 tcctgctata gcgtatgaaa cagataaagt atataaaata accgttctgc atacaaatga
      541 ccaccatgga cacttttggc gtaacaatca tggtgagtac gggttatcct cccaaaaaac
      601 tctggtggac aatatccggc agaaagtaat aaataatggc ggaagtgttc tattattatc
      661 tggtggtgat attaacaccg gtgtacctga atcagacctt cagaaggctg aacccgatat
      721 tcgtggaatg aatctaattg gttatgatgc aatggccgtc ggtaatcatg aatttgataa
      781 tcctctaaat atattacgtc agcaggaaaa atgggccaca tttcctttcc tttctgctaa
      841 catatatcaa aaaagtacag ggaggcgttt attcagtcca tggaaaattt tcatccgtca
      901 gaatttgaaa attgcagtca ttggcttgac gacagatgat actgcgaaaa caggaaattc
      961 tgaatatttt actgatattg agttccgtca acctgctgct gaggcgagga gtgtcattga
     1021 tgaacttaat cagcaggaaa agccagatat tattattgcg gcaacacata tgggtcacta
     1081 tgacaatggc gagtcaggtt cgaatgctcc tggtgatgtt gaaatggctc gcagtttgcc
     1141 gactggctca ttagcaatga ttgtgggagg acattcacaa gctcctgtct gcatggccag
     1201 cgataataaa aaacagtgga attatattcc ggggacaacc tgtgtaccag ataaacaaaa
     1261 tggcatctgg attgtacaag cgcatgagtg gggaaaatat gtcggtcagg ctgattttga
     1321 attctgtaac ggtacaatga aactggttaa ttatcagtta cacccagtta atctcaaaat
     1381 gagaattacc agagaagatg gaaaaacgga attttctttt tacacccccg aaattactga
     1441 agatccacag atgttatcct tactgacacc attccagaat aaaggcaaag cacaactgga
     1501 tgtgaaggtt ggggttgtga acggacgtct ggaaggggac agaagtaaag ttcgttttgt
     1561 tcagaccagt atgggccatc tgattctgag cgctctgaca gaacgtattg acgcagattt
     1621 tgctgttgtc agtggtgggg aaattcgaga ttccatagag agtggtaaca tcacatacaa
     1681 agatatactg aaagttcagc catttggtaa tacggtagta agtattgatt tgacggggaa
     1741 agaagtagct gactacctag ctacagtggc acagatgaaa ccggattccg gagcctaccc
     1801 acaatttttg aataccagct ttgtagtcaa aaaaggaaaa atcgaaatgc taaaaataaa
     1861 aggtaaatcg gttgacctta acaaaaaata tcgtatgaca acatttagct ttaatgctac
     1921 tggtggggat ggctatccac gtattgataa tagacccgga tacataaata ccggatttat
     1981 tgatgctgaa gtacttatcg aatatatccg gaaacattca ccgctggatg ctgcttcata
     2041 tgaaccaaaa ggtgaagtca gttggcagtg ataacgtaat tgacgatcat cacgtgtttt
     2101 ttgctgacag tcgctgtgac tttgtgaaag tagtctctgc gtatgtttgc gatatgggaa
     2161 tgcagttgct gtattttgtg tttctgcttc tgccagttgt tgctgaattt aaccttgcgg
     2221 cttaactgat gttggagcgc tgccagcttc ttcgtcaatt tcgctgatta aagtttattc
     2281 attatggcat gatctgcgta agcggctcgt cagaaccata ttgatattta ctaagagctc
     2341 agatcaactt tccagggcaa cagatcgcgt acccggtttg ccggtcagtc ctggatatgc
     2401 tcaatgacgt aacgcagcca tttttctggc tccacattgt tcagacggca tgtgccgatc
     2461 agcgagtaca acaccgccgc atgttcgcca ccgctatcgg aaccccgccg gagagagttt
     2521 cactctccgg ctgctcacgg aaccgtacgt gacactctcg cgtcatacgg ctcttgatca
     2581 ctcattcgat gcacgtagta aataccagtg ggcaaatagc ttcggatact gtttccttac
     2641 cagcgccagc tttcgctgag ccttccggtt cgaaagtctg tatttagact gcatccactt
     2701 aagcaaacgt gactgcattg cactccacag tcgatagctg aagtttctgg accagaactt
     2761 tccatagtac tcaatccagc ctctcactat cgcattgtag cgcctcgcaa aatccagcaa
     2821 actctcagct gttgagcgat gtatacgcca cttcttgatt gtttctgtta ttttgcacat
     2881 tgctgcattt gatgcacccg gcatacattt tctgtacaat tcgcctttga aattcttcag
     2941 agtacgcact ttgaagtcat atccgagagt ggcatacaac atcatcggcg tacctttcga
     3001 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3061 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3121 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3181 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaacga
     3241 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     3301 caatcgcctg gtgtgcggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     3361 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     3421 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     3481 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     3541 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     3601 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     3661 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     3721 ggggcaagcc ctttgctccg gacccattac ggtccttcac cgctactacg gtttactccg
     3781 tccctgta
//
LOCUS       DAJTRM010000199         3728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRM010000199 DAJTRM010000000
VERSION     DAJTRM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.203"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..224
                     /locus_tag="OO322_004070"
     CDS             <1..224
                     /locus_tag="OO322_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5908710.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO322_004071"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO322_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5908711.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO322_004072"
     CDS             518..1108
                     /locus_tag="OO322_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5908712.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO322_004073"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO322_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5908713.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO322_004074"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO322_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5908714.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO322_004075"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO322_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5908715.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO322_004076"
     CDS             3457..3648
                     /locus_tag="OO322_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908716.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRVIQAAFNAFAKV"
BASE COUNT         1019 a          907 c          935 g          867 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagag taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaaca
//
LOCUS       DAJTRM010000200         3697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRM010000200 DAJTRM010000000
VERSION     DAJTRM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.204"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(49..237)
                     /locus_tag="OO322_004077"
     CDS             complement(49..237)
                     /locus_tag="OO322_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5908717.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            497..832
                     /gene="iraM"
                     /locus_tag="OO322_004078"
     CDS             497..832
                     /gene="iraM"
                     /locus_tag="OO322_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5908718.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1113..1221)
                     /locus_tag="OO322_004079"
                     /pseudo
     CDS             complement(1113..1221)
                     /locus_tag="OO322_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1744..1820)
                     /locus_tag="OO322_004080"
     tRNA            complement(1744..1820)
                     /locus_tag="OO322_004080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1784..1786),aa:Arg,seq:tct)
     gene            complement(1922..1997)
                     /locus_tag="OO322_004081"
     tRNA            complement(1922..1997)
                     /locus_tag="OO322_004081"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1962..1964),aa:Ile,seq:cat)
     gene            complement(2142..2963)
                     /locus_tag="OO322_004082"
     CDS             complement(2142..2963)
                     /locus_tag="OO322_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5908719.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2978..3334)
                     /locus_tag="OO322_004083"
     CDS             complement(2978..3334)
                     /locus_tag="OO322_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5908720.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3347..>3697)
                     /locus_tag="OO322_004084"
     CDS             complement(3347..>3697)
                     /locus_tag="OO322_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5908721.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1052 a          991 c          711 g          943 t
ORIGIN      
        1 gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc actccggctc
       61 agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca gggtataagg
      121 ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat gtgtcagctt
      181 ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa atgccatggt
      241 tctttccaac atctgaaaaa taattcttta aaatacctga catgtaatac agaaaaaaca
      301 caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga taaactacaa
      361 gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga aaaataattc
      421 tgttctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac cagcaaggca
      481 taaacaagga aactaaatga agtggattgt gattgataca gttatccagc catcatgcgg
      541 aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc aatcggatgc
      601 tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc tcaataacaa
      661 agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct ggaatttaat
      721 caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat gctttaataa
      781 ccgttgcact ctgcaaatat gcccttatgg actaaaacaa caaagtccat aaggagttta
      841 ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga gagtggcgct
      901 atgtgccatt ccatggtgcc gggtgcctcc cggtgaattc agtaccagca cctgaatccg
      961 cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt caccatgcca
     1021 gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa ttgtgaagta
     1081 tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt ctttttcagc
     1141 aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg cactgacatt
     1201 atccggctgc tgcaatgcca ttaacaaaaa cagtaagcaa tcacttttta ccgtaacagg
     1261 tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc ataccacagg
     1321 tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta gcaaccatga
     1381 actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct cttcccgaaa
     1441 gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct cgtgctgcat
     1501 tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca atattcactc
     1561 tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga ccgtataaca
     1621 aatactcaat caatacttaa cagaactttt atttttgaca aatatataat attttcaaca
     1681 atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct ggaagaatga
     1741 atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc aattgctctg
     1801 tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga cccgcccccc
     1861 gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg gcggtttgtg
     1921 atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
     1981 cactgagcta aagggccggg agcagaataa taacggtccg taattaattt cgcaataaaa
     2041 aaacccgctc agcggcgggt tgtagaaact cttctaacgc caggcataaa acgcccatca
     2101 ttatgacgaa tttaccacag attccggaaa aatcaacctt gttacctagt tacctttttt
     2161 aactgccgct cagcccatgc ttcttcaata tcaaaccggg tcaccagcgc atcataaaat
     2221 ttcttaactg ttttttccca tgacgcgcgt gttatctggt ttgtcacctc gcatatagca
     2281 ttaaatgcct ccgttgatgg tagtctttca tagccacgac caccacaacg ctggcagtct
     2341 ctgataacag gcataccacg ttttaccgac tcttcacgat gaatggcgac accacgccca
     2401 cggcaatcct tacaggcggt ggaaacctca ccctttccgc cacactccgg acaggcaact
     2461 tttaccacct ccctgacttt tttccattct tcccagtaaa acggatacac acctttcgta
     2521 cactttgccc ataccggcgg cttaccatcc ggatactgga tcttgtttgt aaaaactatg
     2581 ctttcaataa atttttcccc atagcaacaa gggcactgct ttttactcgc tgcgctgcgg
     2641 gcataatcct caaaagcgta cgaagccata atgcgcatca ctaccggttt tatttctgcc
     2701 ggaagttttc tcaacgccgc cacacgatcg caccgactga gtgcataatc tgccagtaat
     2761 tctgttgccc gcgccctgtc attcatactg atgcccattt tccccaggaa cgcagaaaaa
     2821 cccatctcag cccgattctg tgtcatgccc tgcgcggcca tcacatcagt gatactcagc
     2881 gcatcttttg acgttgaggc cgatgcatcg gtcaggccag gggattttgg ggagtagtat
     2941 ttcggtaaat cttccagttt cattttttga cctgctctta atgcattatg gggtaaatct
     3001 tcacccccag acgtccacca gatactggct gaccacgaac gatattgatt tcatcaaact
     3061 gctcatcgtc cattaacact cccgcatgcg tcagcgcatc cagcggtgct ttcaggatat
     3121 tgtccaggtc gcgacgacgc ttatccggtg gctctgcaat cacctttatc gccagccttc
     3181 cggacaggct taatttcagc cgctgctggc gaacaataag cgccacagcc cggcgataac
     3241 gctttccctc ctccgagata aaatatgtgc tgccacggcg tcgccagtaa gtgttcaccg
     3301 tcggcgggta aggtaaaacc aaatctatga gcatcagtca cctcttttac ccaagcacgc
     3361 cagttgcaaa ggcgtgatca agaaaacgaa aaattaaatc aacctgagaa ccatgctttt
     3421 cttcgaacgc cagaggatcc gcatgaagct cgttgtgatg ctcccgacac agcggtagcg
     3481 tgaaaatatc gtgagatttg gtccccattc cgccctgacc atgaccaatc aggtgatggg
     3541 gatcgtcggc tggcttacca caacacgcac acggctgtgt cttcacccag cgtttgtatt
     3601 tctcgttaac ccagcggcga cgtttaggtc gtttcatgaa agattccgga gactcaggat
     3661 caacggcaat gctgaccacc gtcttttcct gttgtgg
//
LOCUS       DAJTRM010000201         3395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRM010000201 DAJTRM010000000
VERSION     DAJTRM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.205"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(16..171)
                     /locus_tag="OO322_004085"
     CDS             complement(16..171)
                     /locus_tag="OO322_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5908722.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(374..781)
                     /locus_tag="OO322_004086"
     CDS             complement(374..781)
                     /locus_tag="OO322_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5908723.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            858..1085
                     /locus_tag="OO322_004087"
     CDS             858..1085
                     /locus_tag="OO322_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5908724.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1069..1491
                     /locus_tag="OO322_004088"
     CDS             1069..1491
                     /locus_tag="OO322_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908725.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1569..2346
                     /locus_tag="OO322_004089"
                     /pseudo
     CDS             1569..2346
                     /locus_tag="OO322_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2353..3099
                     /locus_tag="OO322_004090"
                     /pseudo
     CDS             2353..3099
                     /locus_tag="OO322_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3114..>3395
                     /locus_tag="OO322_004091"
     CDS             3114..>3395
                     /locus_tag="OO322_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5908726.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          877 a          735 c          946 g          837 t
ORIGIN      
        1 tctgtctcgt tcattttact cacctgaatg tcttcccaac caacgacgtg cgccagcttc
       61 ggttttaaac gttttgcttt tggtatacgt tatggcggtg aatgtgccgt cctggttggg
      121 gaacacgccg tacaccggag attcgttgtt gccaagatcg atagtattca tgttgacccc
      181 atttcccctt aacgccgggg tagcggaact gtttgctgag aacaccgtgc ggtgtcttga
      241 tgaataaaat ttagaataac ctaaggaggg tgatcaagat ttttgtgtag aaaaacctaa
      301 gttttttgat gtaaaaaaca caagtgtttg aaagtttgtg ctttttatta cagggtgtgg
      361 agaaaaaagg ggattatttg tttgcgcttc ttttgcgagc tttgagtagt tcttcaaaaa
      421 gtttgttgaa attttcaact cgagcacgca tctctgacaa cagggccttt tgctctgact
      481 caggcagtgc gtcgaacagt tgaagtaact ctttttgatc ttctgtcaga atggctggct
      541 gattatccgg gatcggttcg cctggttgtt tatcttcatc cccaaaaaga agccaagtcg
      601 gtgagcactg aagcgcttgg ctcagtgcga ataatctttt ccccgccggc tgtgtttcat
      661 ctctttccca ttgagaaatt gttacgtgag ccactttgac cagcttacct aatgcggcct
      721 gagacagttt taatttttta cgcctatgta agaggcgagc accgaaggtt tcgtttttca
      781 tattagggaa ttctaatttt tcttgactta ggtttctcta cggcctggtt tccttaggaa
      841 aatctaagga gctcgatatg ttgaaaattg atgctatagc gttttttggc agcaaaacaa
      901 agcttgccaa tgccgcagga gttaggctgg caagcattgc tgcatggggg gaactggttc
      961 ctgaaggtcg cgcgatgcgc ctgcaagagg catctggcgg ggaacttcag tacgacccca
     1021 aagtttatga cgaatatcgt aaggcaaaac gacctgggaa ggtgattcat gaaaatcagg
     1081 catgagcaca tcgaatcagt gctgttagcc ctggcagccg aaaaagggca ggcgtgggtc
     1141 gctaacgcaa ttactgaaga atatctgcgg caggggggcg gcgaattgtc tctggtacca
     1201 ggcaaggact ggaacaatca gcagaatatc tatcaccgtt ggttgaaagg tgaaacgaaa
     1261 gcgcaaaggg aaaaaattca gaaactgatc cctgcggttc tggcaattct tccgcgtgag
     1321 ctgcgtcacc gactctgtat cttcgatacc ctggaacgcc gtgcattact ggcggcgcag
     1381 gaagcgttga gtacggcaat tgatgcgcat gatgatgctg tccaggccgt ttaccggaaa
     1441 gcacatttca gcggtggtgg gtcgcccggc gattctgtcg tagtgcattg attgaaatta
     1501 atcgtgccgg attgttttgt tcggtatcag ttaaatgtaa cgctgcgagc gttacaaggt
     1561 gaaaacaaat ggcttcaaac tggataaagc tcgaggttat tacgccggat aagccggaaa
     1621 tattcaggct tgctgagatt ctgaatattg atccagatgc cgcattaggg aaggttattc
     1681 gcttctgggc atgggcggat caacaaatga tagacggtaa tgcagattgt aacgctcgcg
     1741 gcgttacaaa aagtgcaata gatcgcatca cttttatgtc tggttttgct gatgcgttaa
     1801 ttcaggttgg atggctggtc gaaaatgacg gtgggctttc tctacctaac tttgaacgtc
     1861 ataacggaaa aagctctaaa aaacgggcgg ttacaaacga gcgagtaaca aaaatacgcg
     1921 aactgaaacg aaaaggtaac gctgccagcg ttacacaaac ggatcaaaaa gcgttaccag
     1981 tggaagagga agaggaagat ctaaatactg atctccccct aaatccccct cgccaaaaac
     2041 gagcgtctaa aaaattcgag ccggaggcta ttgagctgcc cgattggttg ccggaaacac
     2101 tctggcatga gtgggtccgg ttcagacagg cattgcgaaa accgattcga acggagcagg
     2161 gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca ggtgattcga
     2221 cacagcatcg ccaatgaata ccagggcctg ttcgcgccga aaggtgttcg gcctgagacg
     2281 ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgcc aggcttcagg
     2341 gggtaacgga ccatgaaaaa tattgcgaca ggcggcgttc tggaacgcat ccgccgactg
     2401 accccgcaac atgtaaccgc cccattcaga acggtagcgg agtggcgcga gtggcaactt
     2461 gctgaaggcc agaaacgtag cgaggagatc aaccgcctga atcgccagtt gcgggtggaa
     2521 aaaattctga atcgctcagg catccagccg ttgcaccgta aatgctcgtt tgcgaattac
     2581 caggtgcaga acgacggcca gcgatacgcg ttaagccagg cgaaatccat cgccgatgaa
     2641 ctgatgaccg ggtgtacaaa ttttgcgttc agcggaaaac ctgataccgg gtagaatcac
     2701 ttagcggcag ctatcgggaa tcgcctgctg aaagacggtc agacagtgat tgtggttacc
     2761 gtggctgatg ttatgagcgc cctgcacgcc agctatgacg acgggcagtc aggcgaaaaa
     2821 tttttgcggg agctgtgcga agtggatctg ctggttcttg atgaaattgg cattcagcgc
     2881 gagacgaaaa acgagcaggt ggtgctgcac cagattgttg atcgccggac agcgtcgatg
     2941 cgcagcgtgg ggatgctgac aaacctgaac tatgaggtga tgaaaacatt gctcggcgag
     3001 cgggtgatgg atcgcatggt catgaacggc gggcgctggg ttaattttaa ctgggagagc
     3061 tggcgtccga atgttagcca ttcgagggtt gttaagtagt ttcaggagga tttatggcga
     3121 aaccttttac tcccgaacag cgggaagaac tgaagacgcg aattgtggaa ctcgtgcatc
     3181 aggacggtcg ggtcacgatt cggcagttgt ccgatgaaac aggtatcagt cgtgcgtctg
     3241 tcggtcgctt atgcatagaa ctggtcgcaa gtggtgatgt atataattct ggctacggct
     3301 tattcccgtc tgaacaggct cgcaaggact ggcaaagcgc ccgcaaaaaa ctctcgagag
     3361 taaaggtgag gaaaccggtt gttgttgatc cggac
//
LOCUS       DAJTRM010000202         3337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRM010000202 DAJTRM010000000
VERSION     DAJTRM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.206"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..688
                     /locus_tag="OO322_004092"
     CDS             <1..688
                     /locus_tag="OO322_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5908727.1"
                     /translation="NGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNAGIALRF
                     RDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHLAVAMAK
                     HIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEVGVQFGS
                     AAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWLSFNWPS
                     YRSRIRGVAA"
     gene            685..2076
                     /locus_tag="OO322_004093"
     CDS             685..2076
                     /locus_tag="OO322_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5908728.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2073..2330
                     /locus_tag="OO322_004094"
     CDS             2073..2330
                     /locus_tag="OO322_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908729.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2425..2625
                     /locus_tag="OO322_004095"
     CDS             2425..2625
                     /locus_tag="OO322_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5908730.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2618..2998
                     /locus_tag="OO322_004096"
     CDS             2618..2998
                     /locus_tag="OO322_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5908731.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3198..3274
                     /locus_tag="OO322_004097"
     tRNA            3198..3274
                     /locus_tag="OO322_004097"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3232..3234,aa:Ile,seq:cat)
BASE COUNT          869 a          735 c          961 g          772 t
ORIGIN      
        1 taatggcgag gacagagaag aaatttcatg ctgccctgac tgcttaatcg ctgaggccaa
       61 cgatttgacc atggagctgt cgtccatcaa ggcggaagag ctgactgata acgccggaat
      121 tgccctgcgt tttcgggact gcgagtttga taattatctg gaggttaatc ctggcgcagc
      181 cagaaatctt gcggcctgtc gccgctatgc ggagaactgg ccagatatgc tggagaacgg
      241 taccagtctt gttatgaccg gcagttgtgg taccggaaaa aatcatctgg ctgtggctat
      301 ggcaaaacac atcatccgta actatctggc cagtgtggag atcaccgacg tgatgcgcct
      361 tacccgtgct gtgaaaaatt gctggcggaa tgacagcgaa aaaacagcgg atgaagttat
      421 tgagcgttat gcgtcaatgg atttgctgat catcgacgaa gttggcgttc agtttggcag
      481 cgcggctgaa atggctattt tgcaggaaat tatcaacgcc aggtacgaaa gtatcttgcc
      541 caccattctg atcagtaacc tctcaccgga agagttgtgg gcgttcatca gtccccgaat
      601 tgccgacagg atcacagacg ggggacgcaa ctggttgtcg tttaactggc ccagttaccg
      661 ttctcgtatc agaggtgtgg ctgcatgaca actccagtat ggcgtaacga tgaccttgaa
      721 ggcgctgtca tcggcgcgtt ctttctgcgc ggagcagatc ctgaagtgat ggatattctg
      781 gccacactgc cagcggacgt tttttctgta cgagcgtatc aggatatcta tacaggcatc
      841 tgcagacagg cccgtgtttc aggagtgatt gaccccgtgc tgttgtgtaa tgagatgccg
      901 gaacttgccc cggtgattac tgataccggg cgtaaaacct gggtgaagtc ttcactggag
      961 cactatgttg cagcgttgcg gcgcaatgcc gcactgcgcg atgcagaaaa aacactgaat
     1021 gaggcgctgc agaaattacg tgatgcgcat acctgtgaag cagctgaaga tgccctgaag
     1081 gatgcgcaga acatgatggt cacactgtcg acaggaaagg gcgtcattca gccggtacat
     1141 attgatgatg tgcttccgga agtggttgag cgtgttgaat gccggaatca ggggctggag
     1201 aaatccagga cgttgatgac cggtattgat gaactggacg caaaaacagg cggtatggag
     1261 cccggagacc tggtattcat tgccgcccgt ccttcgatgg ggaaaaccga acttgcgctg
     1321 gacatcatcg acaaggtgac tgagcagggg catggtgtgc ttctgttcac catggagatg
     1381 gcgaacatcc agattggtga acgtatggtg tctgcggctg gagggatgcc agtatcacgc
     1441 ctgaaatctg tggctcactt tgaagatgaa gactggacgc gtttctcaca aggggtgggg
     1501 cggatgaccg ggcgcaatat ctggatggtg gaccaggcga acctggccat tgatgagata
     1561 tgcgcaacaa cgaaacacca cctgattaaa tatccggaaa cggcgctggt ggtggttgat
     1621 tatctcgggc tgataaaaac ccgaaccacg gggcgtcatg accttgccgt gggtgaaatc
     1681 tcaaaggggc ttaaaggcct ggcaaaatcc ggtggttttc cgttgattgc gctgagccag
     1741 ctctcccgcg gtgtggagtc cagacccaat aaacgtccca tgaactcaga cctgaaaaat
     1801 tccggagaaa tagaggcgga tgcagacatc attctgatgc tttacaggga tgaagtgtac
     1861 aacccggata cgcaggccag gggcatcgca gaaatcaata tcacgaaaca acgtaacggt
     1921 tctctgggaa cgatttaccg gcgtttttat aacggacatt ttctgcctgt ggaccaggaa
     1981 agtgcacggg tgctttccac tcccatgaag cccggcaatc cgcgcagata cagtaataaa
     2041 cgaactgaca gcagtaagat ggagcgtttc ttttgaacaa ccagacaatg acttttaccc
     2101 ctgaacaatt gcgtaaacac gcgcaggaaa tgctgcgtca tgctgaacaa ctcgaaaaaa
     2161 cgggcataac aaaagatgct atccgtaaag atatggtacc cgcgcttcgg gaactgatgc
     2221 aggcgaagca tcgcgcacag aaagcagtag atgagctggt ggactgtgtg gcagagctgg
     2281 aaaccagagt cggaaagttt gaaaaactgg tgcaggaggc gctgcgctga tgcgccatga
     2341 gtttgttttg ccctggcttg tgcgcgatca ttatgtccct ggtggaaagc ccgggataaa
     2401 aatcaccgaa ccggagagta tttgatgagc gtcaaaattc aaacgatacc ggaactgctt
     2461 atccagacac gaggtaatat gaccgaagtt tcacgaatgc tgaactgtaa tcgcgccacg
     2521 gtaagaaaat atgctgaaga taaagagggc aaagggcacg ccattgttga tggtgttctg
     2581 attgttcatc gtggatggga caggggtaaa gacagtgatg cgtgatattc agcaggttct
     2641 tgagcgctgg ggcgcgtggg tggctaataa tcatgaggat gtgacctggt cgtccattgc
     2701 tgccggtttt aagggattaa tcccttcaaa agtaaaatcc cgcccgcaat gctgtgacga
     2761 tgacgcgatg gttatatgtg ggtgtatggc ccgccttaac cggaacaaca acgatctaca
     2821 tgacttgctg gttgactact acgtgtttgg gatgacattc atgacgctgg cgcggaagca
     2881 tggatgctca gatggttata taggaaagaa gctacaaaaa gccgaggggg tggtggaagg
     2941 aatgttgatg atgttgggtg tccggttaga aatggacaaa tatattgaac atccctgatg
     3001 gtaaggggcg actttctaga aaatatttta cgtacgtaaa aaacgggata ttctgttaag
     3061 agtggttgct tcgccacaca gcttaaaccc gcctccgggc gggtttttta tgcctgaaaa
     3121 acggtacagg acgttaaaca cgctggtggt cagatgagtt tgcagatgtg atgacatatg
     3181 gttattattc tgcctccggc cctttagctc agttggtcag agcgagcgac tcataatcgc
     3241 caggtcgctg gttcaagtcc agcaagggcc accaaccacc actagctcat ccggatagag
     3301 catcaacctt ctaagttgac ggtgcgaggt tcgagtc
//
LOCUS       DAJTRM010000203         3334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRM010000203 DAJTRM010000000
VERSION     DAJTRM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.207"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(82..729)
                     /gene="ychE"
                     /locus_tag="OO322_004098"
     CDS             complement(82..729)
                     /gene="ychE"
                     /locus_tag="OO322_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5908732.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1206..>3334
                     /gene="adhE"
                     /locus_tag="OO322_004099"
                     /gene_synonym="adhC"
     CDS             1206..>3334
                     /gene="adhE"
                     /locus_tag="OO322_004099"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5908733.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIA"
BASE COUNT          925 a          824 c          766 g          819 t
ORIGIN      
        1 cttttgataa ctgcgcggga acaaagtaaa aataatgacg aagtaaacgg agcatggcag
       61 ctccgtttca tttgaaagga attaattaag caggccgggg aaaatcccct taataccagt
      121 aacgataaat tcaatcccca atgccatcag caatagcccc ataatacgcg taatcacgtt
      181 gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc atgcggaaca atccccagca
      241 acataaagcg aacaatgcaa tagccacaaa gaaaccaaac agatagctaa tgctgtgata
      301 acgcgtaccc cagacgatgg tagaactgat cgcccctggc cccgccatca acggcaacgc
      361 caatggcacc acaccaatac tttcacgtac cgcggtttct gatttttctt gcttgttctg
      421 tttatcctcg ccaagcttgc cgctgatcat cgacatcgct atcgtcacca ccaggatgcc
      481 cccggcgata cggaacgaat caattgatat accaaaaagt tgtagaatcg tgtcgccgag
      541 aaaaagcgag atccacaaga taatggccac agacaggttg gctgtaaggt tagttttgtt
      601 tcgcgctgcc gctgtctgat aactggtcat gctgataaag acgggaataa tccctaccgg
      661 gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa acgggaaaat caaaaagggt
      721 ctgaatcacg gttcgctccg aagcaaaagc cggataatgt tagccataaa taaggttgaa
      781 aagacgcgct gacaatacgc cttttgacag catttttcac ctcctaacta cttaaaattg
      841 ctatcattcg ttattgttat ctagttgtgc aaaacatgct aatgtagcca ccaaatcata
      901 ctacaattta ttaactgtta gctataatgg cgaaaagcga tgctgaaagg tgtcagcttt
      961 gcaaaaattt gatttggatc acgtaatcag tacccagaag tgagtaatct tgcttacgcc
     1021 acctggaagt gacgcattag agataataac tctaatgttt aaactctttt agtaaatcac
     1081 agtgagtgtg agcgcgagta agcttttgat tttcatcggt taagcaaatc atcaccgcac
     1141 tgactatact ctcgtattcg agcagatgat ttactaaaaa agtttaacat tatcaggaga
     1201 gcattatggc tgttactaat gtcgctgaac ttaacgcact cgtagagcgt gtaaaaaaag
     1261 cccagcgtga atatgccagt ttcactcaag agcaagtaga caaaatcttc cgcgccgccg
     1321 ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat ggccgttgcc gaatccggca
     1381 tgggtatcgt cgaagataaa gtgatcaaaa accactttgc ttctgaatat atctacaacg
     1441 cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga cgacactttt ggtaccatca
     1501 ctatcgctga accaatcggt attatttgcg gtatcgttcc gaccactaac ccgacttcaa
     1561 ctgctatctt caaatcgctg atcagcctga agacccgtaa cgccattatc ttctccccgc
     1621 acccgcgtgc aaaagatgca accaacaaag cggctgatat cgttctgcag gctgctatcg
     1681 ctgccggtgc tccgaaagat ctgatcggct ggatcgatca accttctgtt gaactgtcta
     1741 acgcactgat gcaccaccca gacatcaacc tgatcctcgc gactggtggt ccgggcatgg
     1801 ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt aggcgcgggt aacactccag
     1861 ttgttattga tgaaactgct gatatcaaac gtgcagttgc atctgtactg atgtccaaaa
     1921 ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt tgttgttgtt gactctgttt
     1981 atgacgcagt acgtgaacgt ttcgcaaccc acggcggcta tctgttgcag ggtaaagagc
     2041 tgaaagctgt tcaggatgtt atcctgaaaa atggtgcgct gaacgcggct atcgttggtc
     2101 agccagccta taaaattgct gaactggcag gcttctctgt accagaaaac accaagattc
     2161 tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt cgcacatgaa aaactgtccc
     2221 cgactctggc aatgtaccgc gctaaagatt tcgaagacgc ggtagaaaaa gcagagaaac
     2281 tggttgctat gggcggtatc ggtcatacct cttgcctgta cactgaccag gataaccaac
     2341 cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacagc tcgtatcctg attaacaccc
     2401 cggcttctca gggtggtatc ggtgacctgt ataacttcaa actcgcacct tccctgactc
     2461 tgggttgtgg ttcctggggt ggtaactcca tctctgaaaa cgttggtccg aaacacctga
     2521 tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt gtggcacaaa cttccgaaat
     2581 ctatctactt ccgccgtggc tccctgccaa tcgcgctgga tgaagtgatt actgatggcc
     2641 acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa caatggttat gctgatcaga
     2701 tcacttccgt actgaaagca gcaggcgttg aaactgaagt cttcttcgaa gtagaagcgg
     2761 acccgaccct gagcatcgtt cgtaaaggtg cagaactggc aaactccttc aaaccagacg
     2821 tgattatcgc gctgggtggt ggttccccga tggacgccgc gaagatcatg tgggttatgt
     2881 acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg ctttatggat atccgtaaac
     2941 gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat gatcgctgtc accaccactt
     3001 ctggtacagg ttctgaagtc actccgtttg cggttgtaac tgacgacgct actggtcaga
     3061 aatacccgct ggcggactat gcgctgaccc cggatatggc gattgtcgac gccaacctgg
     3121 ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct ggacgcagta actcacgcca
     3181 tggaagctta tgtttctgta ctggcatctg agttctctga tggtcaggct ctgcaggcac
     3241 tgaaactgct gaaagaatat ctgccagcgt cctaccacga agggtctaaa aatccggtag
     3301 cgcgtgaacg tgttcacagt gcagcaacta tcgc
//
LOCUS       DAJTRM010000204         3205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRM010000204 DAJTRM010000000
VERSION     DAJTRM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.208"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            39..677
                     /locus_tag="OO322_004100"
     CDS             39..677
                     /locus_tag="OO322_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5908734.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            680..1843
                     /locus_tag="OO322_004101"
     CDS             680..1843
                     /locus_tag="OO322_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5908735.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1927..>3205
                     /locus_tag="OO322_004102"
     CDS             1927..>3205
                     /locus_tag="OO322_004102"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5908736.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          711 a          759 c          933 g          802 t
ORIGIN      
        1 tttttaacgc agcttgtatg acatggtaat ggacgatcat ggccagaaaa cgcaaatcaa
       61 gaaataacag taaaattggt cacggagcga tcagtcgcat tggtagaccg aataatcctt
      121 ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac gttggcgcta atgggcggtg
      181 ccgctttttt cgtattgaaa ggttgtagcg atagtagcga tgtcgataac gacggcgacg
      241 gaacatttta cgcgacagtg caggattgta ttgatgacgg taataatgcc gacatttgcg
      301 cgcgtggctg gaacaacgcc aaagcggcat tttatgccga tgttccaaag aatatgactc
      361 agcagaactg tcagtctaag tacgaaaatt gctactacga caatgttgaa cagagttgga
      421 tcccggtcgt ttctggattt ttgttaagcc gggttattcg taaagatcgc gatgagccgt
      481 tcgtctataa cagcggcgga tcctcatttg cttcccgccc cgtctggcgc aacacttccg
      541 gtgattactc ctggcgcttc ggttctggca aaaaagagtc ttactcttca ggcggcttta
      601 ccaccagaaa agcgtcgacc gtttcgcgcg gcggctatgg tcgttcttcc agcgcccgtg
      661 ggcattgggg aggctaatca tgctgagaca caacgttcct gtgcggcggg atctggacca
      721 gatcgccgct aacaacggtt tcgactttca tatcatcgac aatgaaatct attgggacga
      781 gagtcgggct taccgtttta ctctgcgcca gattgaagag cagatcgaaa aaccgactgc
      841 ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg gttaaagacg aagagatcct
      901 gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc gccgaaagct ggcgcgcacg
      961 cgatccttcg ctgtatggcc ggatggattt tgcctggtgt ggcaatgcgc cggtgaagct
     1021 gctggagtac aacgccgata cgccgacctc gttgtatgag tcggcttatt tccagtggtt
     1081 gtggctggag gatgcccggc gcagcggcat tattccgcgt gatgccgatc agtacaacgc
     1141 cattcaggaa cgcctgattt cgcgctttag tgagctttac agtcgggaac tgttttattt
     1201 ttgctgctgt caggacaccg atgaagacag gagtaccgtg ctgtacttgc aggactgcgc
     1261 ccagcaggca gggcaggagt cgcggtttat ctacattgaa gatctcggtt tgggcgtcgg
     1321 cggcgtactg accgatcttg atgataatgt catccagcgt gcatttaagc tgtatccgct
     1381 ggagtggatg atgcgtgacg ataacggtcc gctgctgcgc aagcgtcgcg agcaatgggt
     1441 ggagccgtta tggaaaagta tcttgagtaa taaagggcta atgccgctgc tttggcgctt
     1501 cttccctggt catcctaacc ttcttgcatc ctggtttgag ggtgaaaaat cgcagattgc
     1561 cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc gaaggcggaa acgtcaccat
     1621 tttcgacggt cagaataacg tcgttgacca cgctgatggt gattacgccg atgaaccgat
     1681 gatctaccag gcgtttcaac ctctgccgcg atttggcgat agctacacac tcatcggtag
     1741 ctggattgtc gatgatgaag cgtgcggaat ggggatccgt gaagataaca cactgatcac
     1801 caaagacacc tcacgtttcg ttccgcatta cattgctgga taagaatgtt ttagcaatct
     1861 ctttctgtca tgaatccatg gcagtgacca tactaatggt gactgccatt gatggaggga
     1921 gacacagtgc actggcaaac tcacaccatt tttaatcaac ctataccatt aaataacagc
     1981 aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa cgcgtgaagg tgctggctgg
     2041 gatagcgatt ttctagccag tattggtcag cagttaggaa cggctgaatc ccttgaactg
     2101 gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct acgatgccca ggggcggcgt
     2161 ctggacgatg ttcgtttcca tcccgcatgg cacctgctga tgcaggcgtt atgtaccaat
     2221 cgggtgcaca atcttgcctg ggaagaagac gctcgctccg gcgcatttgt ggcgcgcgcg
     2281 gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt tatgtccagt aacgatgacc
     2341 tttgccgcca cgccattgtt gttacagatg ttacccacgc cgtttcagga ctggaccacg
     2401 ccgctgctga gcgatcgcta cgattctcac ttattgccag gtgggcaaaa acgcggtttg
     2461 ttgattggca tgggaatgac ggaaaagcag ggcggttccg atgtcatgag caacaccacc
     2521 cgtgcagagc gtttggaaga tggctcttat cggctggtgg ggcataaatg gtttttctcg
     2581 gtgccgcaaa gcgatgcgca tctggtgctg gcgcagaccg cgggcggtct gtcctgcttt
     2641 tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga ttcgcctcga gcggctgaaa
     2701 gataagctgg gtaatcgctc taacgccagt tgcgaagtgg agtttcagga tgccattggc
     2761 tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc tgaaaatggg ggggatgacg
     2821 cgttttgatt gcgccctggg tagccatgcc atgatgcgcc gtgcattttc gctggcgatt
     2881 tatcatgcac atcaacgcca tgtttttggt aatccattga tccaacagcc ccttatgcgt
     2941 catgtcttaa gtcgcatggc acttcagctt gaagggcaaa cggcgttgct gtttcgtctt
     3001 gcgcgagcgt gggaccggcg tgccgatgcc aaagaagccc tgtgggcgcg tttatttacg
     3061 cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt ttgtggccga agcgatggag
     3121 gtgctgggcg gcattggtta ttgcgaggag agcgagctgc cgcggcttta ccgggagatg
     3181 ccggtaaaca gtatttggga aggtt
//
LOCUS       DAJTRM010000205         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTRM010000205 DAJTRM010000000
VERSION     DAJTRM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.209"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            102..3005
                     /locus_tag="OO322_004103"
     rRNA            102..3005
                     /locus_tag="OO322_004103"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO322_004104"
     rRNA            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO322_004104"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          702 c          992 g          656 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc catgcgagag t
//
LOCUS       DAJTRM010000206         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRM010000206 DAJTRM010000000
VERSION     DAJTRM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.210"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO322_004105"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO322_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5908737.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO322_004106"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO322_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTRM010000207         3157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRM010000207 DAJTRM010000000
VERSION     DAJTRM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.211"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            98..2095
                     /gene="betT"
                     /locus_tag="OO322_004107"
     CDS             98..2095
                     /gene="betT"
                     /locus_tag="OO322_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5908738.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2158..>3157
                     /locus_tag="OO322_004108"
     CDS             2158..>3157
                     /locus_tag="OO322_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5908739.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSV"
BASE COUNT          732 a          732 c          805 g          888 t
ORIGIN      
        1 cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag gtagtacctt
       61 ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg atacaatccc
      121 aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt tgctaatttt
      181 ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt ttaaactact
      241 tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg ccctgatttt
      301 actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg gcccggacca
      361 tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg cagggatggg
      421 gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt cgccacccgt
      481 tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct ttttccactg
      541 gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt tcttcagtta
      601 ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg gcgatcgcat
      661 atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg tctttggcgt
      721 tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc atcttttcgg
      781 ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg ggttagcgac
      841 gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac tcaatctggg
      901 tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc ttctgctgaa
      961 gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa cgttcaacct
     1021 ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac tgtactgggg
     1081 atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct cccgcgggcg
     1141 aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta cgctaatgtg
     1201 gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg ccacagacct
     1261 cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg agcatttccc
     1321 gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct ttgtaacgtc
     1381 tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg caaacacacc
     1441 cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg cgcttctcct
     1501 tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc ccttctcagt
     1561 gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt tgaccaagcg
     1621 tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa ttccttggca
     1681 gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac gttttatgga
     1741 cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc aggggacgat
     1801 aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt tgggcaacga
     1861 gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct tcgcgctcgc
     1921 cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg ctgaggttta
     1981 tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac agctgattaa
     2041 tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc gttaatagca
     2101 acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga atgcagaatg
     2161 atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt cagggcaacg
     2221 ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa atcaatgata
     2281 cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct gaacatactg
     2341 gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac agcctacggt
     2401 tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga cgtcaccaca
     2461 caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg gattattgcc
     2521 ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt cacactggtt
     2581 gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac ctctctgggg
     2641 agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt tattgcgaga
     2701 gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca gccgaaagga
     2761 acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat ggtgaaactt
     2821 agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc aggcagtttc
     2881 atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc aatcaacacc
     2941 gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga tcctcgattt
     3001 tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt gaacgatcct
     3061 ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta cgcgcagaat
     3121 aaatctgatg aaatagaagt gaaatatcct tcagtac
//
LOCUS       DAJTRM010000208         3148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRM010000208 DAJTRM010000000
VERSION     DAJTRM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.212"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..442
                     /locus_tag="OO322_004109"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO322_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO322_004110"
     CDS             1026..1742
                     /locus_tag="OO322_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908740.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO322_004111"
     CDS             2294..2698
                     /locus_tag="OO322_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908741.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3148
                     /locus_tag="OO322_004112"
                     /pseudo
     CDS             2761..3148
                     /locus_tag="OO322_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          885 a          642 c          713 g          908 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgtt
//
LOCUS       DAJTRM010000209         3137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRM010000209 DAJTRM010000000
VERSION     DAJTRM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.213"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..1652)
                     /gene="flhA"
                     /locus_tag="OO322_004113"
                     /pseudo
     CDS             complement(1..1652)
                     /gene="flhA"
                     /locus_tag="OO322_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(1645..2792)
                     /gene="flhB"
                     /locus_tag="OO322_004114"
                     /pseudo
     CDS             complement(1645..2792)
                     /gene="flhB"
                     /locus_tag="OO322_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          791 a          902 c          760 g          684 t
ORIGIN      
        1 aatcggtact ttttcatcga ggagattttg cagcacttta tgcagtgtgg tgagcgtgac
       61 gacgccagga acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccaacag
      121 ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag
      181 atgcgttgct accaccgtgc tggcctcaac cactgtgtac ccctgaatct gcgcctgttc
      241 ttttagcgca ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc
      301 accaggtaac gtcccggcag cggttccagg gttaatcgcc agccagcgcc ccggataagc
      361 atcaccactg ccaatctcca cgcctttcat caaaatgcga tagcgggcag gttgcagatc
      421 catattgtcg cgaatgtgca ccactggcgg cagaaatccc atctcctggg caaatttctt
      481 gcggatactg cgtatacggc ccaacaactc accatcctgc tggaaatcga ccatcgggat
      541 cagtcgataa cccacttcca ttcccagaga atcttccagt tgtacatcgt tccacgtcgc
      601 ttcgacaacg gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg
      661 ttcgcgtccg cgtatccacc aggccagccc cagcaatccg gcagtgaaca gcaaaaatac
      721 caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat
      781 aacgcttggg ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct
      841 gacacgcgta acgatgcccc ccgcggcggt agaaatcacc agcgccggaa tttgtgccac
      901 cagaccgtcg ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc
      961 agttgcagca cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg
     1021 gcaatggcat cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct
     1081 tcctgagtca cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccagcgtta
     1141 aggtcggcgt caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc
     1201 acttctgcta tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg
     1261 acaaacacca cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg
     1321 accaccttcc cagccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa
     1381 gccacgttaa gtgccagacg caacaacata gtaaacaaca gaatggtcgg aaacgcagca
     1441 aactcaagcg tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata
     1501 ttgaaggtaa acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag
     1561 atcaacagga tcaaaatcgg tccggcaagg atctgccatt atgtcgattt caggtttgcg
     1621 ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag
     1681 ggctgccggc accggaagat gagtaggtgt acagggcgct gtccaccagc cagacgccag
     1741 cgtttcagtt gccagaccca ggccagcact tccgccaccg cggcgtacag ttgacccggg
     1801 atttgttgac caatctccgc atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc
     1861 gtcgggacgt tattttcagc gccaatttca cgaatgcgca gcgcgaccag ccctgcacct
     1921 ttagcgacca ctttcggtgc gctcattttg ttttcgtcat actgcaacgc taccgaatag
     1981 tgggtcgggt tattgacaat gacatccgct ttcggcacat cggccatcat ccgacgccgt
     2041 gcggcagctc gctgcatctg acagatccgt cctttaacat gggggtcgcc ttcgctttgt
     2101 ttgaactcat cacgaatatc ctgccgcgac attcgcagct ttttcaggtg gctgaagatt
     2161 tggaaaaaga cgtcaaatcc caccattgga atgacaccaa gcaccaccag cagtgcgcat
     2221 agccctacca aatccatcgc attacccatg gcggtaatcg gtgactcggc cattaagcgc
     2281 atcatctgcg gccagtgatg ccagagaaaa aaccccgtca cgctgccaac caggatggtt
     2341 ttcaaaattg ccttaagcaa ctccgcgcta gtctgtgccg agaatatccg tttaatgccc
     2401 ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt tgccgctaaa taccagcccc
     2461 cccagcataa ccggagaaat gagcgccacc agcaccacgc cgctaatcag cggtagcagc
     2521 gccagcatgg cttctctgat cagcagaata atttgcccga ggatcagatt cgggtctttg
     2581 ataatactgt gatcaaaatg cagcccagcg gagagcatgc ccgacaatcg acgggccagc
     2641 gacacaccgc caaaccagat aacactaacg cccaccagca aaatcagcag tgaggtcagt
     2701 tcacgggaac gcgggatttg cccctcttcc cgcgcttttt ctagtcggtg aggtgtgggg
     2761 gcttctgttt tgtcgtcgct ctcgtcagac acgtcgccaa tcctggaaag agtattaagc
     2821 gtaaatgatg ccagagcgca aagcgttcaa tggtttaagt aaggggcaaa acaggcggga
     2881 tttagggcgt ttatgcaaaa ataggttcag ttgcagaacg ttttgcgaac cattttccag
     2941 ttctgatatt tagccattta gcctatagaa tcgcactccg cctctctgtt agtttgctaa
     3001 taattaaatt attacaaagg tgatcaaatg gaatggcaac aattgcttaa ctgcaaccgt
     3061 cggaaagata atctaaaaaa aaccaacata gcatttttac atccccttgc tagcggaaga
     3121 caagagatcg agcggga
//
LOCUS       DAJTRM010000210         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRM010000210 DAJTRM010000000
VERSION     DAJTRM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.214"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO322_004115"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO322_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5908742.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO322_004116"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO322_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO322_004117"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO322_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5908743.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO322_004118"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO322_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5908744.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO322_004119"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO322_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          772 a          753 c          758 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag cagggtttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTRM010000211         3038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRM010000211 DAJTRM010000000
VERSION     DAJTRM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.215"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..843
                     /locus_tag="OO322_004120"
                     /pseudo
     CDS             1..843
                     /locus_tag="OO322_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            958..1299
                     /locus_tag="OO322_004121"
                     /pseudo
     CDS             958..1299
                     /locus_tag="OO322_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1282..2119
                     /locus_tag="OO322_004122"
                     /pseudo
     CDS             1282..2119
                     /locus_tag="OO322_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2169..>3038)
                     /locus_tag="OO322_004123"
     CDS             complement(2169..>3038)
                     /locus_tag="OO322_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5908745.1"
                     /translation="NAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTT
                     QASNRIRGLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKL
                     APRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRV
                     LAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGE
                     HPSRRGNKALKRALFLSAFTALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFA
                     MMRDGTFYTPQGS"
BASE COUNT          708 a          753 c          945 g          632 t
ORIGIN      
        1 aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagttctggg ccttgcccgt
       61 agcagttatt tttatcaccg ggctgcactg aaagccggtg ataaatacgc cacgatacgt
      121 aagatgctga cagatatctt taacagtaat taccagtgtt atggctatcg tcgcctgcat
      181 gcgatgctca ggcatgaggg ggggcggcta tcagaaaagg ttgtacgcag acttatggtg
      241 gaggaacagc ttgtcgtcag tcgtaaccgt cgtcgtcgct acagctcata ttgcggagaa
      301 atcggaccgg ctccggataa ccttatcgcc agagatttta aggcggagca acctaatcag
      361 aaatagctga cagatatcac ggagttccag ctccctgcag gtaaagtctg gctgtcacca
      421 gtggtggact gcttcgatgg aaaagttgtg agctggtctc tcagtacacg ccctgatgct
      481 gaactggtca acactatgct ggatagcgct gtcgaaacgt taaatgctgg cgaacgaccg
      541 gtgatacaca gtgacagagg tgggcattat cgctggccag gctggctgga aagagtgaat
      601 gcagcaggtc ttattcgctc aatgtcccgt aaaggatgtt cacctgataa tgctgcatgc
      661 gaaggctttt tcggcagact gaaaacggaa atgtattatg ggcgtaaatg gtcgggcatc
      721 acgccagaaa agttcatgca gcaagtggat gcttacatca gatggtataa cgagcggcgt
      781 ataaaattat cgctgggtgc cgtcagcccc aaaatgtacc gccaacaatg cgggctggaa
      841 tgataaagca gtccaggaaa tcgtccgcat ccccccttca ccatttggat tgtccgctcc
      901 acccaacatg ttgtttcctt aaggttccta caccacaaag gaaactctca tgctgcgcag
      961 tggatggctg acgttgccga caggcgggaa cttcgccagt tcaggcagac accggaacag
     1021 cgcttcacgc aggaacagga gcatctgcag ccgctgctgg gtacggactt cgatatccgt
     1081 catgtctcct gggatggcta tatcgaggtt ggcgggaacc gttacagtgt gccggaaagc
     1141 ctgtgtggtc agctggtatc aatacggatc tcgctggatg atgaactgcg gatctacagt
     1201 aatgagcaac aggtagcatc gcaccgtctc tgttcggctg catacggctg gcagaccgtg
     1261 ccggagcatc acgctccact ctggcagcag gcttcggagc gcatggccga atggctgggg
     1321 gagatacaga aacgggtaat cactgtctgt gaccgggaag cggatatctg gcattacctg
     1381 tattataaag tcagccacgg acagcgtggt gcgtgctgca cagaatcgcc ggctggaaga
     1441 ggcacccggc aagctcttcg aactgccgga agtcctggca accgcaggaa gccacacgct
     1501 gaatgtgatg caaaaaggcg ggcgggcagc ccgtcaggcc cggatgttca tcagttacag
     1561 cgaagtcagc ataaaaaatc ccgacaacag cggccaggcg ctcccgctca cgtatgtctg
     1621 ttgccgggag caggcagagg acggtgcctg ctggcatctg ctgacgtcag gaaaagcggc
     1681 gagtgccgcc gatgcacgac gtattgtcag ccattacgag cgacgctggc tgacagagga
     1741 ataccacaag gcgtggaaaa gtggtggtac atggaatcgc tgcgaatgca gacccgggat
     1801 aaccttgagc gcatggtggt tatcctggcg tttatagcgg tacgggtgct gggcctgcgg
     1861 cagggaggtg tcagtgaaga aacacaaaat gacagctgtg agaaaatact gacgccgaca
     1921 gagtggaaac tactgtgggt taagctggaa ggaaagccgc ttccggtgca ggcaccgacc
     1981 ctgaaatggg cctggggcga tggcatgaca gcaaacgcac aggtcgtccc ggttggggcg
     2041 tcatgtggga tggctggtca gacttcagga tatggttgag ggttacctgg tgatgaagtc
     2101 tcttgatcag gagctctgat caagagacag cagcttaggg gggtgcccct attaagttgt
     2161 caagcatgtt atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga
     2221 acgtcgcagc gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tccctgactc
     2281 attttgcggg tgtagtaagc cctggagagc ggatccctga gcgcggtgaa ggccgacagg
     2341 aacaacgccc gtttgagagc tttattaccc cgtcgcgagg gatgctcacc gcgtattgac
     2401 gagccggatc gccgagttac cggcgcaagg ccagcataag cagcgagatg tgcggcagag
     2461 gcgaaggcgc ggcaggcgac ctcggtgagg agtctggctg cggtcctgac accgactccg
     2521 ggcatactgg tcaggaccgg gtaaagaggg tgagcaagaa ctcgctgttc tacctcaagc
     2581 gccacctcgt ctctttgctt acgcagcgtg atgagctgga gtgccagacg tggcagtact
     2641 acggcagcgg cattcgtgcc gggaacgacg acggtttgtt cggccagtgc ctgagctatg
     2701 tctgctgcaa ggcgtttacc cagacgaggc gcaagtttgc agagctgggc tgccagcttc
     2761 ttctcaccca gcgaagcgag tttttctggt gagggatatc gctggagaag atcgagtacc
     2821 gccgggtgct caagtctcgg accgagaacg cgctccggtg ccggatgtat ctgggtcaga
     2881 aggccgcgga tacggttgct ggcctgcgtt gtctgtgcgg caagatcatc atcgaagccg
     2941 cagagcatgg agagttcggc gatttgctcg tcagccagtt tcagcgtgcg tagcgcgtga
     3001 ggcagggtac gggcagcttc ggcaatgatg gcagcgtt
//
LOCUS       DAJTRM010000212         2976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRM010000212 DAJTRM010000000
VERSION     DAJTRM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.216"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            103..930
                     /locus_tag="OO322_004124"
     CDS             103..930
                     /locus_tag="OO322_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5908746.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            974..1723
                     /locus_tag="OO322_004125"
     CDS             974..1723
                     /locus_tag="OO322_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5908747.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1720..2289
                     /locus_tag="OO322_004126"
     CDS             1720..2289
                     /locus_tag="OO322_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908748.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2413..2655
                     /locus_tag="OO322_004127"
     CDS             2413..2655
                     /locus_tag="OO322_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5908749.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2659..2805
                     /locus_tag="OO322_004128"
     CDS             2659..2805
                     /locus_tag="OO322_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908750.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2814..>2976
                     /gene="xisR"
                     /locus_tag="OO322_004129"
     CDS             2814..>2976
                     /gene="xisR"
                     /locus_tag="OO322_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5908751.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          801 a          718 c          784 g          673 t
ORIGIN      
        1 gaaggatacg gtagcagtga tttctggctt tgctctggcg tgtctgaccc cgccctgcat
       61 ttatgccatt tataagggtc tttctttttc taaggataaa agatgaaaaa agttattcct
      121 gctttagccc ttgttctgct gaccaccgga cttgtcggtt gcgatcgcgt tgagcctggt
      181 aatgttggca tcaaggtaaa caaactgggc gacgataaag gcgtcggaga agtggtcggt
      241 gttgggcgct actggacggg atggaacact gaggtttaca tcttcccgac cttcaaacaa
      301 atgaagacct acgatgagcc attcagcttc cagatgagtg acggtacaac catcggctat
      361 cacatcggcg tggcctacaa agttgatcca tccaaagtta ccacagtctt tcagacctac
      421 cgcaaaggcg tggacgacat taccgacacc gacctgcgcc agaagatagc cgacgcactc
      481 aaccgactgg ccagcaaaat gaccaccgac aaatttatcg acggcggcaa gtctgagctg
      541 ctggatgcag ctcttaaaga cattcaggaa gaaatgacgc ccatcggtat tcaggtaatg
      601 agcctctcat atgtgggtaa gccggagtac ccgcctactg ttatcgacag cattaatgcc
      661 aaagtcacgg cgaaccagaa aaccctgcaa tgcgagcagg aagtaaaaca gcgcgaagcg
      721 gaagccaaca tgttgcgcgc ggaagctgcc ggacaggcag atgcgattcg cacaaaagcc
      781 caggccgaag ccgatgctat tcgtttacgc ggtgaagctc tgcgccagaa cccaaacgtt
      841 atggagctgg aagccatcaa caagtggaac ggtacactgc cgcaatacat gaccagcggt
      901 gccaacacac catttattca ggttaagtaa tacatatgcc cggtattaca cgccgggctg
      961 tctggagata aaaatgaata ctgtaaccat caacaacaaa cagtttccgg taatcgaata
     1021 tcgcggtcag cgtgttgtga cgctggcgat gattgatgaa gtccaccagc gaccggaagg
     1081 aaccgcgcgt gcagcattca accgaaaccg tgagcatttc atcagcggtg tggattatgc
     1141 cgaattaggt gcggacgtaa tacgtacgga cctcccggaa ggaacattct ctaaatttgc
     1201 accatcgggg attgtacttt ttgaatcagg ttacctgatg ttgacgaagc cattcaatga
     1261 cgcccttgcc tggcaggttc agcgcgaact ggttaacagc tacttccgca ctcgcacacc
     1321 gctaacggaa atcgagatga tcgctgcgat agccgccgac gctgttcgtc aacagaagcg
     1381 cctgaatcat gtggaagaac agctcgaaac agtcacagaa gcggtggaaa ccatcaaacg
     1441 cggaaatatg cgcgccggat atgtcggtta ccgccaggtg gtagccaaaa gtggaatgag
     1501 tgacaccaag tgtcggaatc tggtgaacgc atatcaaatt cctacagaca cccacgaatt
     1561 catgactccg gacggtctgt tgtctcgcag ggctatcgtc gagcttgagc catttatggc
     1621 ggcgttccac caaatgatgt cagaggctga cccacgcggc acacgctggt atcacccgaa
     1681 gatggggctt ttccaggtga tcggatggga ggataaagca tgatcatcca gtcaaaactt
     1741 attcgcgccg ctctggtgtg cgctgctaaa aacgacgttc gttattacct gaacggtctt
     1801 cacatcacgc cgaaacatat tgaggcaacc aatggttccg tggcactgcg catggctcac
     1861 ggcatccgga cgaagaaaaa catcattgtc cagttcgaag ggggcgtccc ggccaaagcc
     1921 gaaacgacag agctgatttt tagtaaagag ccgatcgctg ttcatcgcga ccagtttcag
     1981 cgacgactgt ccattaccgg cattaaactg gtggacggtt gttttccgga tttggatcgc
     2041 atcattccga aaaagtttga ccgctgtact cacccggtgc tccaggcggg ttacctgagt
     2101 tatccagaga agatgtttgg tcgtgagcgt aaatttattc ccgtccagtt acgtccctcc
     2161 ggtgacgggc aagcggtcag aattcagttt gattccatca tcaactcaat gtatggcaat
     2221 cctgaatttg ttgtgatgcc ttgtcgtgat catggcgatt tcaacgtggc tcaggagcat
     2281 ccggaatgaa aatcgaatac caggacgccg caggaggtga atcaatgagc tggcctgatg
     2341 caatcgtaac tctggggatg gtattcgcag tagcgtttgt tgtgtactcg atttgtcgat
     2401 ggggataacc acatgttcgc tttgattcaa cgcgggcaga tatacacaga cagagccgga
     2461 tacccagtag aaattactcg cagtactgag cactcagtgt tctttcggag gatggacgga
     2521 cgtaccgggc gagtacgcat cggtgaattc agtagcctgt tcgagcatat tgaccacctg
     2581 gaatatcaca agattctggc tgaaacagaa caagaaaagc acctgaagaa attacgcgca
     2641 atgcaaagga agtaaaaaat gaataaagcg tttgagctat gggtccacca gcgttacggc
     2701 aaccactacg acctgacgcg agatgttgac ggtttctact gtcgtgaaat tgtgaaacga
     2761 atgtttgagg tctggtgcca ctgccgtggg ctgagtgttg tgtgaggtaa tgcatgggca
     2821 atgtgattca actggctccc aatgaatggg tttgtgaaag cgttctaatc gcaattaccg
     2881 ggctcaaacc aggcacaatt cttcggaccc ggaaagaatg ctggatggtt ggaagagagt
     2941 atattcacgt atcaccagac ggtaatccaa agcctt
//
LOCUS       DAJTRM010000213         2776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRM010000213 DAJTRM010000000
VERSION     DAJTRM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.217"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..687
                     /gene="lsrF"
                     /locus_tag="OO322_004130"
     CDS             <1..687
                     /gene="lsrF"
                     /locus_tag="OO322_004130"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5908752.1"
                     /translation="PTIGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNKPVVLR
                     ASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMK
                     VGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIV
                     IAGGKKLPERETLEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADR
                     AYELYLSEKQ"
     gene            711..1001
                     /gene="lsrG"
                     /locus_tag="OO322_004131"
     CDS             711..1001
                     /gene="lsrG"
                     /locus_tag="OO322_004131"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5908753.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1040..1798
                     /gene="tam"
                     /locus_tag="OO322_004132"
     CDS             1040..1798
                     /gene="tam"
                     /locus_tag="OO322_004132"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5908754.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1802..2716)
                     /locus_tag="OO322_004133"
     CDS             complement(1802..2716)
                     /locus_tag="OO322_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5908755.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          729 a          646 c          744 g          657 t
ORIGIN      
        1 ccgactatcg gacttgaacg cattgatata aatatcgccc cgctgtttga acatgccgat
       61 gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc ccccggcgac caataagccg
      121 gtggtactgc gggcgtcagg tgcgaactct attctggcgg aattaagtaa tgaagccgtg
      181 gcgttatcga tggatgatgc cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat
      241 atcggcagcg aatatgaaca tcagtcgatc aaaaatatta ttcagctggt cgatgccgga
      301 atgaaagtgg gaatgccgac catggccgtg actggcgtgg gcaaagatat ggtgcgcgat
      361 cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa tgggggcgca aattatcaaa
      421 acctattatg tcgaaaaagg ttttgaacgg attgttgccg gatgtccggt acccattgtt
      481 attgctggcg gtaaaaaatt accggagcgc gagacgctgg aaatgtgctg gcaggctatc
      541 gatcagggcg cttctggtgt ggatatgggg cgtaatattt tccagtctga ccatccggtg
      601 gcgatgatga aagccgtaca ggcggtggtt caccataacg aaacggctga tcgggcatat
      661 gaactctatc tgagtgaaaa acagtaactg cggatttaag gagaagaatt atgcacgtca
      721 cactggttga aattaacgtt catgaagaca aggttgacga gtttatcgaa gtttttcgcc
      781 agaaccacct gggctctgta caggaagaag gtaatttgcg cttcgatgtc ttacaggacc
      841 cggaagtgaa ttcgcgcttt tatatttacg aagcctataa agatgaagac acagtggcat
      901 tccataaaac cacgcctcac tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg
      961 ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg aggctacaat tggggtaacg
     1021 cgctgacggg agtaaaaaaa tgtctgactg gaacccctct ttatatctgc acttttccgc
     1081 tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga atgtcgaata
     1141 tgttgccgat cttggctgtg gcccaggtaa cagcaccgcc ctgctgcatc aacgttggcc
     1201 tgcggccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc
     1261 tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg aacaggcact
     1321 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     1381 tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc cagataactg
     1441 gctggagccg acccatgtgc tcatgcgcga agttgcttgg gaacaaaact acccggatcg
     1501 cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg aagccggatg
     1561 tgaggtcgat atctggcgaa ccacttacta tcaccagatg ccgtcgcacc aggcgattat
     1621 cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     1681 gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac tgcaagagaa
     1741 cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaatt
     1801 atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct gcttcatcgt
     1861 tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     1921 tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa ataaaagtgt
     1981 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg
     2041 gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag gcaaagggaa
     2101 ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc tgaaatatca ttaagccgat
     2161 cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga accgccaacc
     2221 attctcccat tattaacaag atacggttag ctggagagtt cgaagccagt acacgctgaa
     2281 gatctccagt cttgagataa tgagccagca cttccgcctg tggctgtttg cgtaatgtca
     2341 tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa ctcccttaca cttgccgaat
     2401 ccggcaatgt cgtttttacc tctcgcagta acgaccgtga ggcaatcatc aactgccccc
     2461 aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa ccaagaaaaa
     2521 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg cccagatgcg
     2581 tgtaccaggg cagcatgaat ataacagcaa tagaaaagag aaaattgagt agtaagcgcg
     2641 aggatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt
     2701 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
     2761 cgatcacatt ctcaag
//
LOCUS       DAJTRM010000214         2760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTRM010000214 DAJTRM010000000
VERSION     DAJTRM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.218"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..146
                     /locus_tag="OO322_004134"
     CDS             <1..146
                     /locus_tag="OO322_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5908756.1"
                     /translation="ALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYA
                     SMA"
     gene            303..1052
                     /locus_tag="OO322_004135"
     CDS             303..1052
                     /locus_tag="OO322_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5908757.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1074..1640
                     /gene="yieF"
                     /locus_tag="OO322_004136"
                     /gene_synonym="chrR"
     CDS             1074..1640
                     /gene="yieF"
                     /locus_tag="OO322_004136"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5908758.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1694..>2760)
                     /gene="adeP"
                     /locus_tag="OO322_004137"
     CDS             complement(1694..>2760)
                     /gene="adeP"
                     /locus_tag="OO322_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5908759.1"
                     /translation="MGLNAFFAFVVVQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYW
                     MIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANSETLVSIGNLTSHSVLLGILGFF
                     IIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNL
                     GLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGKFPRMKQALYVDSISSVTGSFIG
                     TSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGV
                     LMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIALGFISYCVMKIGTGRLRDLS
                     PCVIIVALLFILKIVFIDAH"
BASE COUNT          762 a          733 c          679 g          586 t
ORIGIN      
        1 ttgctcttcc tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtt ccttttaccc
       61 tgctgtggca taaacggaac agccataatc cgaagatcgt ctggttacgg gaaaccatta
      121 aaaaccttta cgcgtcgatg gcataaccga atcgtatgaa acgcgaccca atttcacaat
      181 aaaatgtaaa aaagttgtaa taagcttgtc tgaatcgaac ttttagccgc tttagtctgt
      241 ccatcattcc agtaaatgat tactcttgta ttcataatgg accattaagc atggagcgaa
      301 aaatggcgac tcactttgcc cgagggattt taacggaagg acatctgatt tctgttcgtc
      361 tcccctccca gtgtcatcaa gaagcccgaa acattccccc tcatcgtcaa agccgttttc
      421 tggcgtccag aggtttactc gcagaactga tgttcatgct gtatggcatt ggcgaattgc
      481 cggaaatcgt caccctgccg aaaggtaaac cggttttcag tgataaaaat ttgccttcgt
      541 tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg
      601 gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata
      661 accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag
      721 cgcgggcgca gctcattacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga
      781 atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg
      841 taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca
      901 cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc tggaaaagcc
      961 tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc
     1021 tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg
     1081 aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg
     1141 ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca
     1201 ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg
     1261 ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat
     1321 ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg
     1381 atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc gtgattggcg
     1441 gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga
     1501 acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag
     1561 tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta
     1621 ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa
     1681 tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa
     1741 cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt
     1801 aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg
     1861 cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg
     1921 aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagtct ggcaccattc
     1981 ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca
     2041 gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag
     2101 aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca
     2161 tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg
     2221 taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga
     2281 ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggcgcag
     2341 aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga
     2401 tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg atgaaaaagc
     2461 cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg
     2521 agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac aggccgatac
     2581 cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc
     2641 ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct
     2701 gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg
//
LOCUS       DAJTRM010000215         2741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRM010000215 DAJTRM010000000
VERSION     DAJTRM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.219"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1806
                     /gene="kup"
                     /locus_tag="OO322_004138"
     CDS             <1..1806
                     /gene="kup"
                     /locus_tag="OO322_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5908760.1"
                     /translation="GDIGTSPLYTLRECLSGQFGFGVERDAVFGFLSLIFWLLIFVVS
                     IKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGEVVITPAI
                     SVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILA
                     GLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGGEVLYADMGHFG
                     KFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALAT
                     VIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIV
                     SFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFT
                     ANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPV
                     RVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQL
                     SPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKRPWYLRL
                     RGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            1973..2392
                     /gene="rbsD"
                     /locus_tag="OO322_004139"
     CDS             1973..2392
                     /gene="rbsD"
                     /locus_tag="OO322_004139"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5908761.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2400..>2741
                     /locus_tag="OO322_004140"
     CDS             2400..>2741
                     /locus_tag="OO322_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5908762.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKID"
BASE COUNT          606 a          659 c          725 g          751 t
ORIGIN      
        1 ggcgatattg gtaccagccc gttatataca cttcgtgaat gtttgtccgg ccagtttggt
       61 tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc tgatcttctg gctgctaatc
      121 tttgtggttt ccattaaata tctcaccttc gtgatgcggg cagataacgc cggtgagggg
      181 gggatcctga cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac cacatcaatg
      241 ctggtgatta tggggctaat cggcggcagc tttttctatg gtgaagtcgt cataacaccc
      301 gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca gctggatacc
      361 tggatagttc ccctctcaat tatcgttctc actttattat ttatgattca aaagcatggc
      421 acggctatgg tcggtaagct gtttgcgccg atcatgctga cctggttttt gattctggca
      481 ggactggggt tacgtagcat tattgctaac ccggaagtgc tgcatgcgct gaatccgatg
      541 tgggcggtgc atttcttcct tgaatacaaa acggtttctt ttattgcatt aggggcagtg
      601 gtgctgtcga ttacgggggg cgaggtgctg tatgctgata tggggcactt tggtaagttc
      661 tctattcgcc tggcgtggtt caccgtcgta ttaccttcct tgacccttaa ttacttcggt
      721 cagggagcgt tgttgttaaa gaacccggaa gcgattaaga acccgttctt cctgttggca
      781 ccggactggg cgctgatccc actgctgatc atcgccgcac tggcgacggt aattgcctcg
      841 caggcggtta tctctggcgt cttctcattg acgcgtcagg cggtacgtct gggatatttg
      901 tcgccgatgc gcattattca cacctccgaa atggagtcag ggcaaatcta tattccgttt
      961 gtgaactgga tgctctatgt cgcggtcgtg attgtgattg tcagctttga gcactccagc
     1021 aacctggcgg cggcgtacgg gattgcggtg accggaacca tggtactgac gtctattctc
     1081 tcgactaccg tggcacgtca gaactggcac tggaataagt attttgttgc gctgatcctg
     1141 attgctttcc tttgtgtcga tattccattg ttcaccgcta acctcgataa actgctctcc
     1201 ggcggctggt tgccattgag cctcggtact gtgatgttta tcgtgatgac cacctggaag
     1261 agcgagcgtt tccgcttgct gcggcggatg catgaacatg gtaactctct ggaagcgatg
     1321 attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt gtatatgtcg
     1381 cgtgcaatca acgtcattcc ctttgcgctg atgcataacc ttaaacataa caaggtattg
     1441 catgagcggg tgattctgtt aactctgcgc accgaagacg cgccatatgt ccataacgtc
     1501 cgtcgggtac agattgaaca actgtcgccc actttctggc gcgtggtggc aagttatggt
     1561 tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga aggattaagt
     1621 tgccggatga tggaaacctc cttctttatg tcgcatgagt cgttgatcct cggcaaacgc
     1681 ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa tgcgctgcgt
     1741 gcaccagatc aatttgaaat cccgccaaac agggttatcg aactgggtac tcaggtcgaa
     1801 atctaacgcc agacgcctcc tttcttcata agggggcgtt tttgttttca tggttaatca
     1861 ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt tttcccaact
     1921 cagtcaggat taagctgcgg atcagcgaaa cgtttcgctg atggagaaaa aaatgaaaaa
     1981 aggcaccgtt cttaattctg atatttcatc ggtgatctcc cgtctgggac ataccgatac
     2041 gctggtggtg tgtgatgctg gtttacccat ccccaaaagt acaacgcgta tcgatatggc
     2101 attaacccaa ggtgtacctt cttttatgca ggtgctgggc gtcgtcacaa atgaaatgca
     2161 ggtcgaggct gccattatcg cggaagagat caaacaacat aatccgcaac tccacgaaac
     2221 gttgctcact caccttgagc agctgcaaaa acaccaggga aataccattg aaattcgtta
     2281 caccacgcat gaacaattca aacaacaaac cgcagaaagt caggcggtaa ttcgcagcgg
     2341 agaatgttct ccgtatgcga atatcattct ctgtgctggc gtgacgttct gaggccgtca
     2401 tggaagcatt acttcagctt aaaggcatcg ataaagcctt cccgggcgta aaagccctct
     2461 cgggcgcagc gttaaatgtc tatccgggcc gtgtgatggc gctggtgggc gaaaacggcg
     2521 cgggtaaatc caccatgatg aaagtgctta ctggtatcta tactcgcgat gccggcacgc
     2581 ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga
     2641 ttgggattat ccatcaggaa ctgaacctga tcccgcagtt gaccattgcc gaaaacattt
     2701 tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga c
//
LOCUS       DAJTRM010000216         2723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRM010000216 DAJTRM010000000
VERSION     DAJTRM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.220"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO322_004141"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO322_004141"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5908763.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO322_004142"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO322_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5908764.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..>2723
                     /gene="ssuD"
                     /locus_tag="OO322_004143"
     CDS             1599..>2723
                     /gene="ssuD"
                     /locus_tag="OO322_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5908765.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIP"
BASE COUNT          623 a          747 c          734 g          619 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccc
//
LOCUS       DAJTRM010000217         2721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRM010000217 DAJTRM010000000
VERSION     DAJTRM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.221"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..197
                     /locus_tag="OO322_004144"
     CDS             <1..197
                     /locus_tag="OO322_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5908766.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO322_004145"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO322_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5908767.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO322_004146"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO322_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5908768.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO322_004147"
     CDS             1821..2576
                     /locus_tag="OO322_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5908769.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          677 a          630 c          676 g          738 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc acggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac t
//
LOCUS       DAJTRM010000218         2648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRM010000218 DAJTRM010000000
VERSION     DAJTRM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.222"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..546)
                     /locus_tag="OO322_004148"
     CDS             complement(<1..546)
                     /locus_tag="OO322_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908770.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2197)
                     /locus_tag="OO322_004149"
     CDS             complement(1208..2197)
                     /locus_tag="OO322_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908771.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2281..>2648)
                     /locus_tag="OO322_004150"
     CDS             complement(2281..>2648)
                     /locus_tag="OO322_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908772.1"
                     /translation="LLTTSSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNK
                     RAEAERIAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGR
                     SRAAIVADLESGGNWMDSL"
BASE COUNT          692 a          615 c          637 g          704 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gagttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa gacaggtgaa
      841 tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac ttgaagcacg
      901 taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt ttggtctggc
      961 tcaggtcagg ctatgggggc ttttggctcg ctctttatat tagatgaggt ctcacatttt
     1021 tcagtcaaaa ctcatcgtct aatagcccat agccagacca ccagtcccag ctttagcagg
     1081 aagaccacga gatagacttt gtgtaggtcc atttggccct cctactttct gtattcacaa
     1141 actagattac tagtacaaaa taaaggccag acatccagtt cggaagtctg accaatagtc
     1201 gttattgtta gttagcttct ttagtgaagc tcaggcgttt gactacaggc aggacagcag
     1261 tagccgagcc agtacccatc ttgatggtcg tagcagcttc agctacaaca aacaccttgc
     1321 cgagaatctc aaggtcgtga gactgggagt ctccatcacg tgaaaggacc acagtaatgc
     1381 tctcgccatc gtgacggaag caaccgaagc tcagagccgt aaaacgctta gccatcatga
     1441 acacgccacg gtcttcaccg ttgtaatagg cgacgtcagt ccccagcata tcgacgatag
     1501 cgtcttcacc accgaagccc tgcttagcag ccagacggga gagagccttg aggtgtgact
     1561 catgggcgat aatgccgtag tcagaaacct cagaaccgaa agaagactcc gtgttctcgt
     1621 acagggcatc catgatagcg tcagccttag cggtcaggct accagtcagg gccggaagtt
     1681 ctacggtctt cagcagcttt gaggactcca gcacgtcaac catcttagag ttaatggtac
     1741 gaatcacgtt atcgcagatg ttcagtgcgt cctgctggag cgtcccctga gtgaaaccag
     1801 tggtcgactt ctggtcccaa agctttgttt ggtgagacac gaggatcgcc tgttcaatcg
     1861 gctcaacggt gatttcagtg ccagacggag cggtcacaac ttcaggtcgc ttaccaccac
     1921 ggacagggac cagcacagag cggtcagcca gagtgtgagc aatgattgga gtcggtaagc
     1981 tgttacgggt cggcaggact tctacaggga acgggatgtt atcttgagcg tctttaagta
     2041 caccgttcag catagcctta acagtcttag ctaactggag ggcatagacc tgagggtcgg
     2101 cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct
     2161 caatgccaaa ctggaaagcg tttttagtca tattcattat aattaatctc cttgagtcaa
     2221 atacttgaag tccctaacag gcagtcaggg accgtgtgtt ctcagatgag ggacgcttgc
     2281 ttacaagctg tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacgtga
     2341 gcgaccagcg gaatgagtag cgctgcggag gttttcacca taggtccggc gttcagcttt
     2401 gacctgttcg cggcgctgta gctctttttg ccattcgtct tcggtcatga acatttcgcg
     2461 ggtacgctct ttctgagacg ctagctcttt agcgatacgc tcagcttcag ctcgtttgtt
     2521 catctggcgg cgttggtagg cggtcatcag gtcattactg aagaagttac caatggtcac
     2581 gccagcttca gccagagctt ccagtaactc catcgcctcg cggctggtgg atgaagtggt
     2641 caacaaaa
//
LOCUS       DAJTRM010000219         2646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRM010000219 DAJTRM010000000
VERSION     DAJTRM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.223"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..751)
                     /locus_tag="OO322_004151"
     CDS             complement(<1..751)
                     /locus_tag="OO322_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908773.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVD"
     gene            complement(751..2169)
                     /locus_tag="OO322_004152"
     CDS             complement(751..2169)
                     /locus_tag="OO322_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5908774.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(2170..>2646)
                     /locus_tag="OO322_004153"
     CDS             complement(2170..>2646)
                     /locus_tag="OO322_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5908775.1"
                     /translation="LVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMMAEWLGGDV
                     SPGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTAR
                     YGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
BASE COUNT          633 a          691 c          657 g          665 t
ORIGIN      
        1 tatccacgct gaagaatgta gctaacagga tgttgtcggt tgttgtcgcc gcattacgac
       61 tgctgaccag cgtatcagga acagagccgg aaaaggttag ctgcattgac ctgttggcgg
      121 ttccgctggg ccacgtcccg acgatcgaca gcttgaagaa caaggttttg ttctcgttga
      181 acacaaccat cttgttgtta acggtgtcga agaatggtgc caacgtgccg gatgacggcg
      241 tgagcgtttt cagtaggcta acaaggttgg tcggcgctgt cgggatggtt acagatacgc
      301 cagagtaaac aacctctgac ttcttgcgag tagtggcata ctccagagca tcgatgcgcg
      361 tttcatggtc tgaaacctgc gattccagcg actgaactct ggtatcaagc gacgcaatat
      421 cgctttcatt ctgagcgatt cgcgtttcat gttcctgaag agttgattct gcctggctga
      481 ttcgctcctc atgattaaca agcgttgctt ccgcagcaga aattcgctgc tcatggtcag
      541 cgagaatcac atcctgctca tcgttcctga cttgtgcgtc ataagcgccc tgtccggcct
      601 cgttggcctt gttggccacg ttaccaacat cagtgccctg tgcgataacg taaagcagat
      661 acgactgcga gaagatattg cgtggaagga ctgatgtgtc gagccgtgta gcctgaatga
      721 ttaccggcac attgagattc gaatccgcca ttactcaatc cttatctggc agccagacag
      781 agtgacaggt gacttcgtga taacgcgcaa tttgaagccg acatttttcc tgatgcgccc
      841 tactcgcttc cacaaaacgc gtttgtcgta aacgaacggt tcattctgct caatcatctg
      901 ctcacacccg taattgatgc cgtcagtggt tgcagagaga aaaaggcggt cagcatactg
      961 cgcaacgcca gttgacgatt caacctcaag gtcgaacact ctggcgttat ccgctttgaa
     1021 cagtggagta aacagcaggt gttcctgttg cttgtcgtac tggctgctga tgtcgaattg
     1081 caatttcccg gtcacggact ccagcttatc gccgcacgtt atttgattgc cttcgtaaat
     1141 gaagtcgata gcgcggtaca catcgtcata caggcctgtt ttcagcacac accattgcgg
     1201 accattggcg cttgaagatg cgtcgtacac cagtacatgg cgcggaaggt ggataatcag
     1261 caactcatgc gcatcaaacc gcagcgattc catcacgcca tcagccagtt catcagcagt
     1321 gtaggagcgg aggattttct caatgctcgc gctggcgatt ggtgacacct gaccggagcc
     1381 gatgatgtat acagacggcg cacctgttgc cggattgctg atgaacgcat aagaatcagc
     1441 gaatggtgtt ttgcagtaag tcccggcaat acctttctgc accatcagcg atggctgtgc
     1501 gacatacaaa gcggcaccaa cggtggttgc acctgtcagg gaaaaatatt caatcgtcga
     1561 tgagccaaag cagacgatga agtctcgcca tgtacctatg ccgatgatgc cgtccggctg
     1621 agactcggca cgatattgtg cactgtagcg gtcaggatgc gattcatctt caaggtcagt
     1681 gataaaccat gaatcagtgc cgtcttttga ccacgcataa cgcccacgta agcgcgtaat
     1741 gtcgcgaact gaacctaact catactgagt gaatccactg tctgtaggcc agtttgagac
     1801 ggttttaacc gtgccatcat agcgatactc gaccagttga ccattaacgc ctaccgcctg
     1861 tgatgtccga ccatgcgcca ttgatacgcg accacttccg gcaacatcac cgacctcgct
     1921 ttctccctta tacaacttgc caccgcaaac gcgataaaca gcattctgcg ccatgttgta
     1981 ctcgacaccg cgcgatacac cattcacatc agaacgtttg gcaatgcccg ggaatgagcg
     2041 aagatatccg ctgctgttga gtatttcttt gggtgtagcc agcatattca ctggcagata
     2101 gtcgatgtag tcggcgtttc ggaaatcttt gccgacacct ttcataagcg gaagttgctg
     2161 aatcggcatt tattcgcccc cgttatcgca aggttccttc cggtggaagt aattccaacc
     2221 attccacttc gccaactggt taccgctacc aacaggcata cggtttggat aaccggactt
     2281 acatttagcg gcttttgctc tgtccattgc agacagtttg acgagtcgct ctttcccgta
     2341 tctggcagtg gttataagtt ttgctggcgc ttccagcgca taatctggag caatgcggca
     2401 ggcaaggttg aaaatgacgg cattgatagc gttatttgat aaaccgtgct catcgcccgg
     2461 ttctggagcg acatctgcat cagcgaaaat gtagccaacg ttgataccag gtgacacatc
     2521 accgccaagc cattcagcca tcatcatttc aaggtcgttg acgccgtctt ccatagactg
     2581 cggttctaca tcggttaacg tggcatttga tgccacaccg agcttacgta atgccgcaag
     2641 aactaa
//
LOCUS       DAJTRM010000220         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRM010000220 DAJTRM010000000
VERSION     DAJTRM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.224"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(64..822)
                     /locus_tag="OO322_004154"
     CDS             complement(64..822)
                     /locus_tag="OO322_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5908776.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO322_004155"
     CDS             complement(830..1933)
                     /locus_tag="OO322_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5908777.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO322_004156"
     CDS             complement(1943..>2572)
                     /locus_tag="OO322_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5908778.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTRM010000221         2492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRM010000221 DAJTRM010000000
VERSION     DAJTRM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.225"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..182
                     /locus_tag="OO322_004157"
     CDS             <1..182
                     /locus_tag="OO322_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5908779.1"
                     /translation="GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEIT
                     PGEADAVVNVTLDYR"
     gene            262..957
                     /locus_tag="OO322_004158"
     CDS             262..957
                     /locus_tag="OO322_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5908780.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            986..>2492
                     /locus_tag="OO322_004159"
     CDS             986..>2492
                     /locus_tag="OO322_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5908781.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQF"
BASE COUNT          732 a          592 c          549 g          619 t
ORIGIN      
        1 gcggaaacct tatgccactg aatgctatgg ggaatgataa cacgatttat cagatcccag
       61 cggactccaa tggtattgtc aatgttgacc tcatcgctta ctacgtatca accgttgaag
      121 catcagaaat caccccaggg gaagcagacg ctgtcgttaa cgtaacgctg gattaccgtt
      181 aatacgttac ggcgttatct gacctttcag ataacgccct tttccttcct ctttctcgtt
      241 gtatcaggtt gaaaaataac tatgtcaaaa cgaacattcg cggtgatatt aaccttgttg
      301 tgtagcttct gtatcggcca ggcgcttgca gggggtatcg ttttacagcg aacgcgagtg
      361 atctatgatg ccagccgcaa agaggctgcg ttacctgtcg caaacaaagg cgcagaaacg
      421 ccttatttac tgcaatcatg ggtagataat atagatggta aaagccgtgc cccatttatt
      481 ataaccccac cgctatttcg tcttgaggct agcgatgact catcactgcg aattattaaa
      541 acagctgata acctgcctga aaataaagag tcgctgttct acattaatgt tcgtgccatt
      601 ccagcaaaga aaaaatcaga tgatgttaat gctaacgagt tgacgctggt atttaaaacg
      661 cgggtcaaaa tgttttatcg ccccgcacac ctgaagggac gggtaaacga tgcgtggaaa
      721 tcactggaat ttaaacgtag tgaccattca ctcaatatat ataacccaac tgaatattac
      781 gtcgtatttg ccggactggc agtcgataaa accgatctca caagcaaaat tgaatatatc
      841 gcgcccggag aacataaaca gttaccactt ccagcatctg gcggaaagaa cgtgaaatgg
      901 gctgcgatca acgattatgg cggcagttcc gggacagaaa ctcgtccact gcaataaaaa
      961 atataaaaac acaggccatc agggaatgcc acaacgacac caccagggac ataaacgcac
     1021 accgaaacag ttggcgctca ttatcaaacg ttgtttgccg atggtgctca ctggcagcgg
     1081 catgctttgc actaccgcta acgccgaaga gtattatttc gaccccatta tgctggaaac
     1141 tacaaaaagt ggtatgcaaa caaccgatct gtcacgtttt tcaaaaaaat acgcacaact
     1201 accaggaact tatcaggttg atatctggct gaataaaaag aaggtttcac agaaaaaaat
     1261 tacatttacc gccaatgcag agcaacttct gcagccacag tttacggtag aacaactacg
     1321 tgagctgggt attaaggtgg atgaaatccc ggcgctggct gaaaaagatg acgatagcgt
     1381 gatcaactcg cttgaacaaa tcattcccgg tacagctgct gaatttgatt tcaatcatca
     1441 gcgacttaat ttgagcattc cccaaattgc actgtaccgt gatgcaagag gttacgtctc
     1501 cccttctcgt tgggacgatg gtataccaac gctgtttacc aactactcgt ttacaggttc
     1561 tgataaccgt taccgccagg gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg
     1621 tgctaatttt ggcccctggc gattacgtaa ctattctacg tggacacgca acgatcaggc
     1681 gtcaagctgg aacactatca gtagttattt acaacgtgat atcaaggcgt tgaagtctca
     1741 gttgcttctg ggagaaagcg ccaccagcgg cagtattttt tccagctacg cctttactgg
     1801 cgtgcaactc gcttccgacg ataatatgtt gccaaacagc cagcgcggat ttgccccaac
     1861 ggtacgcggt atcgcaaaca gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat
     1921 ctatcaaagc aacgtgccag cgggtgcctt tgaaattaac gatctctacc cctcttccaa
     1981 cagcggcgat ttagaagtca cgattgaaga aagtgacggt acacaacgtc gctttatcca
     2041 gccttattct tcattaccca tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc
     2101 tggacgctat cgcgctgatg caaacagtga tagcaaggaa cccgaatttg ctgaagccac
     2161 ggcaatatat ggtttgaata atacttttac gctgtatggc ggcctgctcg gttctgaaga
     2221 ttattatgcg ctggggatcg gtatcggcgg cacacttggc gcactgggcg cgttgtcgat
     2281 ggatatcaac agagctgaca cccaattcga taaccagcac tcttttcatg gctatcaatg
     2341 gcgtactcag tacatcaaag atatcccgga aaccaacacc aatatcgctg ttagctacta
     2401 tcgctatacc aacgatggct attttagttt tgatgaagcc aatacccgca attggggcta
     2461 taacagtcgc caaaaaagtg aaattcaatt ca
//
LOCUS       DAJTRM010000222         2446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRM010000222 DAJTRM010000000
VERSION     DAJTRM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.227"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1312
                     /gene="mppA"
                     /locus_tag="OO322_004160"
     CDS             <1..1312
                     /gene="mppA"
                     /locus_tag="OO322_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5908782.1"
                     /translation="AKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQA
                     IIDGKATPDQLGVTAVDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKP
                     GNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGD
                     IDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDR
                     RLMTEKVLGTGEKPAWHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQK
                     PLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRAS
                     WVGDYNEPSTFLTLLTSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKIL
                     MEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1363..2394)
                     /gene="ynaI"
                     /locus_tag="OO322_004161"
     CDS             complement(1363..2394)
                     /gene="ynaI"
                     /locus_tag="OO322_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5908783.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          723 a          556 c          562 g          605 t
ORIGIN      
        1 cgcaaaatgg gcggatggca caccggtaac ggcgcaagat tttgtctaca gctggcaacg
       61 tctggtggac ccaaaaacat tgtcgccatt tgcatggttt gccgcgctgg cgggaatcaa
      121 caacgcacag gcgattattg atggtaaagc tacgcctgat cagcttggcg tcaccgcagt
      181 tgatgcccat actttgaaaa ttcagcttga taaaccgttg ccgtggtttg tgaatttaac
      241 cgctaacttt gccttcttcc cggtgcaaaa agccaacgta gaaagcggta aagagtggac
      301 gaaacccgga aatctgatcg gcaatggcgc ttatgttctt aaagagcgcg tagtcaatga
      361 aaaactggtc gtggtaccga atacccatta ttgggataac gccaaaacgg tgctgcaaaa
      421 agtgaccttc ctgccaatta atcaggaatc cgcagccact aagcgttacc tcgcggggga
      481 tattgatatc accgaatcct tcccgaaaaa tatgtatcag aagctgttga aggatattcc
      541 ggggcaggtt tatacgccac cgcagctcgg gacctattat tatgccttta acacgcaaaa
      601 agggccgacg gcagatcagc gcgttcgcct ggcattaagt atgacgatag atcgccgcct
      661 gatgaccgaa aaagtgttgg ggacgggcga aaagccagcg tggcatttta taccagatgt
      721 taccgccgga tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg aagaactgaa
      781 tgcgcaggca aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac cgctgaagct
      841 gacgcttttg tataacactt cagaaaacca tcaaaaaatt gcgattgctg tagcatcgat
      901 gtggaaaaag aaccttggcg tagatgttaa attgcaaaat caggaatgga aaacctatat
      961 cgatagccgt aacaccggca attttgatgt tatccgcgcc tcatgggtgg gggattataa
     1021 tgaaccctcc actttcctga cattattaac gtcaatgcat tcaggaaata tttcacgctt
     1081 taacaatccg gcatacgaca aagttctggc ccaggcatcg acggaaaata ccgttaaagc
     1141 gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg gagcaagcac cgattgcacc
     1201 aatttatcaa tataccaatg gacgattaat caaaccgtgg ctgaaaggtt atcccattaa
     1261 taaccctgaa gatgtagcgt acagtcggac tatgtatatt gtgaagcatt gatgtgatgg
     1321 gaactggcgt tacccttgtg gataacgcca gtgatgtctg atttagcgcc cctgttccgg
     1381 cggtgtaatg ttatccatat acagcgtctg gctggggaag gcaaagtccg cgccgtgtga
     1441 ctgtacaata tcgataatct tcaaataaac gtcttgctgt gcagcaagcc actcagccca
     1501 taccgtggtt ttggtaaagc aataaaccat aatattcaat gacgagtcag caaactggtt
     1561 gaaataaacc agtaaggttt gtcgctggtc gatggccggg tgatttttca gcatctcacg
     1621 tacggcttcg acaataacgc ccacttttgc cgcatcctca taacgtaaac caatggtcgt
     1681 ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg ctgatcgacg aaaacagcga
     1741 gttcggtacg tacaatggac gattatcaaa ggtcgtaatt ttggtaattc gccagccaat
     1801 ttccgctact gtaccttcga tatttctgtc cggtgaacgg atccagtcgc caatactgaa
     1861 aggacggtcg aaatagagca tgatcccgga aaagaaatta ctcagaatat ctttaccggc
     1921 cataccgaca gccagaccac caataccacc aaaggtcagc aagccagaaa ggctcatgcc
     1981 gaaatgttcg ccataaagca gaacaagcac tacaataatg gtgattttga taatgcgcga
     2041 cataatccgc gcactggtga tatcgcgacc ttttttaatc tgctgttttt caaactgatt
     2101 aatcagcaga aatagcttaa tcgtcagaat aaccgcaatc agggacgtac agataaaatc
     2161 gataacgcct ggggtgataa atttgagttt atagttttct ataacataat tactgatgct
     2221 accaacagca ctgataatta tggtgtagat taaaaattgc accgcatgga ataaaaatcc
     2281 ttttctttta cgatttccac ggcgaaacca aaagctcatc agaatcaatg ctgcgcagct
     2341 accgaaaata atgaccagat taagcgcatt atttgtaaac agttcagcga tcattgtttt
     2401 atcaggctcc tccagataat tgtcgtcatg ccggaaaccc ctggcg
//
LOCUS       DAJTRM010000223         2402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRM010000223 DAJTRM010000000
VERSION     DAJTRM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.228"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            97..1302
                     /gene="tet(B)"
                     /locus_tag="OO322_004162"
     CDS             97..1302
                     /gene="tet(B)"
                     /locus_tag="OO322_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5908784.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1415..2008)
                     /gene="tetC"
                     /locus_tag="OO322_004163"
     CDS             complement(1415..2008)
                     /gene="tetC"
                     /locus_tag="OO322_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5908785.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2096..>2402
                     /locus_tag="OO322_004164"
     CDS             2096..>2402
                     /locus_tag="OO322_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5908786.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     "
BASE COUNT          649 a          419 c          502 g          832 t
ORIGIN      
        1 atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta
       61 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
      121 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
      181 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
      241 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
      301 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
      361 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
      421 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
      481 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
      541 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat
      601 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
      661 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
      721 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
      781 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
      841 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
      901 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
      961 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
     1021 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     1081 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     1141 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     1201 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     1261 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     1321 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     1381 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     1441 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     1501 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     1561 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     1621 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     1681 tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt
     1741 ctaaggcaga ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa
     1801 ttaattgctg cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa
     1861 aggcaccttt ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc
     1921 ctttttcaac aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat
     1981 tttcttgttg atgattttta ttttccatga tagatttaaa ataacatacc gtcagtatgt
     2041 ttatggtatc atgatgatgt ggtcgtgaca atcttaagaa catttaggtt attttatgta
     2101 tattgaacag cattctcgct atcaaaataa agctaataac atccaattaa gatatgatga
     2161 taagcagttt catacaacgg ttatcaaaga tgttctatta tggattgaac ataatttaga
     2221 tcagtcttta ctgcttgatg atgtggcgaa taaagcgggt tataccaagt ggtattttca
     2281 gcggctgttc aaaaaagtaa caggggtcac actggctagc tatattcgtg ctcgtcgttt
     2341 gacgaaagcg gctgttgagt tgaggttgac gaaaaaaact atccttgaga tcgcattaaa
     2401 at
//
LOCUS       DAJTRM010000224         2318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRM010000224 DAJTRM010000000
VERSION     DAJTRM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.229"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..118)
                     /locus_tag="OO322_004165"
     CDS             complement(<1..118)
                     /locus_tag="OO322_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5908787.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHST"
     gene            complement(129..>2318)
                     /locus_tag="OO322_004166"
     CDS             complement(129..>2318)
                     /locus_tag="OO322_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5908788.1"
                     /translation="VDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYI
                     SPTRWDEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLN
                     TSDGRAEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPAS
                     QNGFAPVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEE
                     DGSEQRFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLY
                     GGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDD
                     TDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNL
                     YLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQ
                     IYMLSLSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSH
                     DDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIA
                     LVKAPGAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVV
                     TMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGE
                     LYLSGLPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
BASE COUNT          610 a          585 c          504 g          619 t
ORIGIN      
        1 gcgtactgtg aaatacgcca ttatcagggg tacagatacc tttaggagct gcaagtgcta
       61 cactacttaa cgagagcata aacacagcaa agcaccactg tttgatatac ttgttcataa
      121 aaatcctttt aatgacatgt cacaccctgt tcaattaatc cgctctcact ttctggtgtc
      181 gaaaatgaat aattgaagat gcattttgtt gaggcgtttt ctccccagga taacgtaacc
      241 tgtccttttt ctggtagacc agaaagataa agttcgccac ggtcaccgac aattcctgaa
      301 tctgccaaac ttgcatcttg tatcgcagca atggctccaa aaggtaccgg tttaccattt
      361 gcatttaaca ctctgaagag aacgcgataa cccacatgag tcacaaactc cgctttaacc
      421 actgcaccac gcgtgggaac catcgtaacg acggtattat caagttctat attcgacttc
      481 gagaaatagt tatcgctgat tgctacccga ttttcatcat aagggtttag ctgtgtcttg
      541 accgtatagc cgcgccagtc cgtcgcagca ccgcgcatat tatctatttc taacctggcg
      601 gccccaggtg ctttaacaag agcaatagtt tcacctaact cctgacttaa cgttacccct
      661 tcgctgtgtg ccagaattcc ccctctgata ccataattca gcctctggga attttggctg
      721 tagttatagc tgccgttcac cgtgccataa gagccatcat aggtcaggga catattaccg
      781 ccaacctcat catcatgact gttataggac tgcattaaat tccatgaaag gttatttctt
      841 tccagcgccg taccgctgag tccgagttga tgtgagctac cggaattatc gttttgcgaa
      901 acagaatagg tggcgtaact attaccccac cagcccgata aaggaacaga taaagagagc
      961 atgtagatct gctcactggt gttatcactg gcatggttgt tccccgtccc ctgtctggca
     1021 gtattggaat aactccagga gacgttccac gagagacgct tccagctact gctgtagcca
     1081 aattgcatta atcgttcgac atcatcggta tgccagtatt gttgccggct taatgtcagg
     1141 tataaattgc cataatctct ccccaacgac tgcgttagcg ttccctccac gcgactacgt
     1201 cggttacctg tttcccaaaa gtcttcaggg ctgttgcgcc gcgatgccca ctcattgagg
     1261 gtatagtagc cctcggtgga gtaacgatag ccaaccaggc gcaagctagt gtctgtgtcg
     1321 tcaaatagtt ttgaatagag aaagcgatat gactgacccg tttctgtatc atcatggcta
     1381 aaattcgcac gagcatgtgt cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta
     1441 ccgacaccca gagctgcaga accataattt tcagcagcct gcatacctcc atataaagtg
     1501 atcccgtggg aaaaaccatg aagtatttgc gcctgaagga cgtccggtgt aaatccgtct
     1561 tcatcgcgca attcacccac gctgacatca acatctgtca gaccttcacg tttaagaatc
     1621 gccaatgaag cataaggttg ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt
     1681 acgtccaaat cgccacctgt acttgcggtg ttgagatcgg tgatctcaaa agcaccttgt
     1741 ggaacggctg actgataaat cacgtagcca ttctgccgaa tgatgacggt ggcgttggac
     1801 tttgcaatcc cacgaaccac aggagcaaag ccattctggc tggcgggtag catatcgtta
     1861 tcagtataca aacgcgcgcc gcgaatttgc gtactgtcga aaatatcgct cgccgtccac
     1921 gtatcaccaa tcataatctg gcttcttaac gcggcaatat cgcgctgtat ccaggtacta
     1981 atggagttgt attccgcacg gccatcgctg gtgtttaaag tactgtaatt acgtagccgc
     2041 cacggtccaa tattcagtcc attgcgcaga ttgagataac tggtatctgt ctcttgtgaa
     2101 ccataatctg aacgtagtgt atgtgaacca gaaaaatcgt agttgatggt gagcgcatta
     2161 atcccctcat cccaacgcgt ggggctgatg taatcataag ccatcgcgtc catgtagatt
     2221 tggtgaatgg atatattcag ctgcaattta tcactgtcaa actgccagga tgagccttca
     2281 atacgtggag caggatcgag gcaaaccgtc tcatccac
//
LOCUS       DAJTRM010000225         2310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRM010000225 DAJTRM010000000
VERSION     DAJTRM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.230"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..893
                     /locus_tag="OO322_004167"
     CDS             <1..893
                     /locus_tag="OO322_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5908789.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..>2310
                     /locus_tag="OO322_004168"
     CDS             894..>2310
                     /locus_tag="OO322_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5908790.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSR"
BASE COUNT          635 a          588 c          601 g          486 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg
//
LOCUS       DAJTRM010000226         2268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRM010000226 DAJTRM010000000
VERSION     DAJTRM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.231"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO322_004169"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO322_004169"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5908791.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          576 a          561 c          668 g          463 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactcta
//
LOCUS       DAJTRM010000227         2234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRM010000227 DAJTRM010000000
VERSION     DAJTRM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.232"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(148..660)
                     /locus_tag="OO322_004170"
     CDS             complement(148..660)
                     /locus_tag="OO322_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5908792.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            855..1619
                     /locus_tag="OO322_004171"
     CDS             855..1619
                     /locus_tag="OO322_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908793.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1570..>2234
                     /locus_tag="OO322_004172"
     CDS             1570..>2234
                     /locus_tag="OO322_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5908794.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          640 a          486 c          563 g          545 t
ORIGIN      
        1 accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt ggggggtagc
       61 ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg ggggaagctg
      121 gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga agctaaaact
      181 accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat acactttcac
      241 tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca cggcgataaa
      301 gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa cccgttctat
      361 tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa ttattatacc
      421 aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga ttccagcttc
      481 ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa ttgatgctgg
      541 tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa aaagaagccc
      601 ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc actccatcaa
      661 tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt aatttaggct
      721 taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag cgtcgcagga
      781 gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa cagtaaatca
      841 caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca taccgggccg
      901 gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg gctatcagga
      961 agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc aaaaatggta
     1021 cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt acacaaaaaa
     1081 gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga atgcgtaccg
     1141 atcccccgac taacccattc caacccggta accagcaggc attaaaacac ggtggttatg
     1201 cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg acgctcgagg
     1261 acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt ggtcggtggt
     1321 tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt gaaaatatca
     1381 gcgccgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc gttggcacgc
     1441 ttgcgacggt aggaaaaata tttgctgata cagcctaccg caaggccgcc actgataagg
     1501 tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat tgatgatggc aatggagagc
     1561 gtgacctcaa tgacttctac tctgacatcc aaaccgacgc tgaatccggc tttacgtagt
     1621 ttctggacta tgcgggcacg taacaaagtg ctttatggtg gtcggtcatc gtcaaaatca
     1681 tgggatgccg ctggcattgc catatttctg tcgaataaat acaccctgcg tttttgttgt
     1741 gcccgtcaga tccagaataa aatcgaagag tcggtgtata ccctgctcaa aattcagata
     1801 gacaggtttg gtctgcggca ccgtttccgt attctgaaca acaaaatcat taaccgggtt
     1861 actggctcgg aatttgtttt ttatggatta tggcgcaaca tcgaagaaat taagtcactg
     1921 gaggggatcg atatgttgtg gttggaagaa gcccacgcac tgacggaata ccagtggaaa
     1981 attctggagc caacgatccg taaagagggt tcggaatgct ggttcatatt caaccccgga
     2041 cttgttactg atttcgtctg gcgcaacttc gttgttgatc cgcccgaagg cactctcatc
     2101 cgcaaaatta actatgacga aaatccgttt ctgtctgaca ccatgcttaa ggttatcgac
     2161 gcggcgcgac gccgtgatcc ggatggtttt aaacatgtgt atgagggcgt tccggagtct
     2221 gatgatgatg cggc
//
LOCUS       DAJTRM010000228         2208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRM010000228 DAJTRM010000000
VERSION     DAJTRM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.233"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..948
                     /locus_tag="OO322_004173"
     CDS             <1..948
                     /locus_tag="OO322_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908795.1"
                     /translation="TDSWFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCF
                     GSSTIEYFSLTGATTVGAALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPS
                     VYIIDSGQVSPIASASIEKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDA
                     SSIANGPQWCVLKTGLYDDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQ
                     EHLLFTPLFKADNARVFDLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFV
                     YDKRVLWKRVGRIRKNVGFKLRVITKSPVTLSGAQIRIE"
     gene            948..1796
                     /locus_tag="OO322_004174"
     CDS             948..1796
                     /locus_tag="OO322_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908796.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            1796..>2208
                     /locus_tag="OO322_004175"
     CDS             1796..>2208
                     /locus_tag="OO322_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5908797.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSA"
BASE COUNT          562 a          567 c          545 g          534 t
ORIGIN      
        1 actgattcat ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca
       61 caatatcgtg ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc
      121 atcgtctgct ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt
      181 ggtgccgctt tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact
      241 tactgcaaaa cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg
      301 ccgtctgtgt atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag
      361 aaaatcctcc gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga
      421 tttgatgctc atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca
      481 tcttcaatcg ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg
      541 taccgcgcta tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa
      601 tctgttaccg ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac
      661 ctgctgttta ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa
      721 tcgtcaactg gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc
      781 atcaattacg ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt
      841 gttttgtgga agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc
      901 acgaagtcac ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga
      961 atctcaatgt gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca
     1021 atatcttctc gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg
     1081 tggctaacaa agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg
     1141 agcaggatgt gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg
     1201 ttaatcatga ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa
     1261 tagctcagaa tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc
     1321 aggtttcaga ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt
     1381 cagaggttgt ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta
     1441 gcctgctgaa aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta
     1501 acaacaagat ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg
     1561 gcacgtggcc cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc
     1621 ctgacacact ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta
     1681 cgttcttcag cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc
     1741 agtcgaatgg tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat
     1801 tacattcaaa ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat
     1861 tatcgccggg agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga
     1921 ggtgaacgtg cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac
     1981 attagattgc cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct
     2041 ggcgttctgg cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc
     2101 acgcaaattc cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac
     2161 catcaagaaa tacttcaaag gcgtggatga cgtgactttt tacagcgc
//
LOCUS       DAJTRM010000229         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRM010000229 DAJTRM010000000
VERSION     DAJTRM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.235"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            274..578
                     /locus_tag="OO322_004176"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO322_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..>2094
                     /locus_tag="OO322_004177"
     CDS             747..>2094
                     /locus_tag="OO322_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5908798.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSL"
BASE COUNT          539 a          416 c          482 g          657 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctgg
//
LOCUS       DAJTRM010000230         2052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRM010000230 DAJTRM010000000
VERSION     DAJTRM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.236"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            6..224
                     /locus_tag="OO322_004178"
     CDS             6..224
                     /locus_tag="OO322_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5908799.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO322_004179"
     CDS             226..591
                     /locus_tag="OO322_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5908800.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO322_004180"
     CDS             588..1253
                     /locus_tag="OO322_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908801.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO322_004181"
     CDS             1253..1618
                     /locus_tag="OO322_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5908802.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          559 a          425 c          545 g          523 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc ga
//
LOCUS       DAJTRM010000231         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRM010000231 DAJTRM010000000
VERSION     DAJTRM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.237"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(210..374)
                     /locus_tag="OO322_004182"
     CDS             complement(210..374)
                     /locus_tag="OO322_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908803.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1276..1560
                     /locus_tag="OO322_004183"
     CDS             1276..1560
                     /locus_tag="OO322_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5908804.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..>2032
                     /locus_tag="OO322_004184"
     CDS             1548..>2032
                     /locus_tag="OO322_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5908805.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          523 a          427 c          582 g          500 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc agtgaagctg
     1621 tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1921 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1981 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa ta
//
LOCUS       DAJTRM010000232         2019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRM010000232 DAJTRM010000000
VERSION     DAJTRM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.238"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            11..1360
                     /gene="ccp"
                     /locus_tag="OO322_004185"
     CDS             11..1360
                     /gene="ccp"
                     /locus_tag="OO322_004185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5908806.1"
                     /translation="MCYLGLSGYVWYHDNKRSKQADVQASAVSENNKVLGFLREKGCD
                     YCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAALLADKPVSQSDLNKIEW
                     VMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYASNDTAPEHRNEPVQPI
                     PQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGVDGRKTSIGVGGAVGPI
                     NAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQLKAQ
                     FLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLFKDNK
                     CATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGLRNVA
                     LTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            1571..>2019
                     /locus_tag="OO322_004186"
     CDS             1571..>2019
                     /locus_tag="OO322_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5908807.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAG"
BASE COUNT          546 a          419 c          523 g          531 t
ORIGIN      
        1 tggcgtcgcg atttgctatt tagggttatc tggttatgtg tggtaccacg ataataaacg
       61 cagtaaacag gccgatgttc aggcatctgc tgtcagtgaa aataataagg ttttaggctt
      121 tctccgcgaa aaaggatgcg actattgcca cacgccttcg gcagaattac ccgcctatta
      181 ttatattcct ggcgcgaaac agttgatgga ttacgacatt aagcttggat ataaatcttt
      241 taacctcgag gccgtgcgtg cggcactgct ggctgataaa cccgtttcgc aaagcgatct
      301 gaataagatt gaatgggtga tgcagtatga aactatgcca ccaacgcgtt ataccgcgct
      361 acactgggcg ggtaaggtga gtgatgaaga gcgggcggaa atactggcct ggattgcaaa
      421 acagcgcgcg gaatattacg ccagcaatga tactgctccg gagcatcgca atgaaccggt
      481 gcagcccatc ccgcaaaaac tgcctaccga tgcgcaaaaa gtggcgttgg gctttgcgct
      541 gtatcacgat ccccgtttat cggctgatag caccatttca tgcgctcatt gccatacgtt
      601 gaatgcgggg ggcgtcgatg gcagaaaaac atcgattggt gttggtggcg cagttgggcc
      661 gattaatgcg ccgacggtat ttaactcagt atttaacgtt gagcagttct gggatggtcg
      721 tgcggcaaca ttgcaggatc aggcaggtgg accgccgttg aacccgattg aaatggcgtc
      781 gaaatcctgg gacgaaatta ttgctaagct ggaaaaagat ccgcagctta aagcgcagtt
      841 cctcgaagtt tatccgcaag gtttcagtgg cgaaaatatt actgatgcca ttgctgaatt
      901 tgagaaaaca ttaattacgc cggattcccc atttgataaa tggttgcgtg gagatgaaaa
      961 tgcgctgacg gcgcaacaga aaaaaggcta tcaattattt aaagataata aatgtgcaac
     1021 ttgtcatggt ggtattattc tcggcggacg ttcctttgaa ccgttggggc tgaaaaaaga
     1081 ctttaacttt ggggaaatta cggcggcgga tattggtcgt atgaatgtga ctaaagaaga
     1141 gcgtgataaa ttgcgtcaga aagtacccgg tttacgtaac gttgctttaa cggcaccgta
     1201 cttccatcgc ggtgacgtgc cgacgctgga cggggcggtg aaactgatgc tgcgctatca
     1261 ggtaggcaaa gagctgccgc aggaggatgt ggatgatatc gtagctttcc tgcacagtct
     1321 gaacggggtg tacacgccgt atatgcagga taaacaataa ttaatttgat cgcccgaaca
     1381 ggaatgtttg ggcgattttt attacgataa taaagtctgt ttttaatatt atcatgttaa
     1441 atgtttatat tataaaaagt cgtttttctg cttaggattt tgttatttaa attaagcctg
     1501 taatgccttg cttccattgc ggataaatcc tactttttta ttgccttcaa ataaatttaa
     1561 ggagttcgaa atggaccaga agctgttaac ggatttccgc tcagaactac tcgattcacg
     1621 ttttggcgca aaggccattt ctactatcgc ggagtcaaaa cgatttccgc tgcacgaaat
     1681 gcgcgatgat gtcgcattcc agattatcaa tgatgaatta tatcttgatg gcaacgctcg
     1741 tcagaaccta gccactttct gccagacctg ggacgacgaa aacgtccata aattgatgga
     1801 tttgtcgatc aataaaaact ggatcgacaa agaagaatat ccgcaatccg cagccatcga
     1861 cctgcgttgc gtaaatatgg ttgccgatct gtggcatgcg cctgcgccga aaaatggtca
     1921 ggccgttggc accaacacca ttggttcttc cgaggcctgt atgctcggcg ggatggcgat
     1981 gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaa
//
LOCUS       DAJTRM010000233         1994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRM010000233 DAJTRM010000000
VERSION     DAJTRM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.239"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            10..96
                     /locus_tag="OO322_004187"
     tRNA            10..96
                     /locus_tag="OO322_004187"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44..46,aa:Leu,seq:cag)
     gene            131..217
                     /locus_tag="OO322_004188"
     tRNA            131..217
                     /locus_tag="OO322_004188"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Leu,seq:cag)
     gene            complement(256..492)
                     /locus_tag="OO322_004189"
     CDS             complement(256..492)
                     /locus_tag="OO322_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5908808.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            633..1421
                     /gene="fhuF"
                     /locus_tag="OO322_004190"
     CDS             633..1421
                     /gene="fhuF"
                     /locus_tag="OO322_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5908809.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(1459..>1994)
                     /gene="bglJ"
                     /locus_tag="OO322_004191"
     CDS             complement(1459..>1994)
                     /gene="bglJ"
                     /locus_tag="OO322_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5908810.1"
                     /translation="DAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVI
                     VDDDIEARLIGSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQS
                     RTLSPTEREILRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAED
                     ILLCMRHCEASNVLHPY"
BASE COUNT          522 a          528 c          457 g          487 t
ORIGIN      
        1 tcgcactggg cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgttctt
       61 acggacgtgg gggttcaagt cccccccctc gcaccaaacg aggcgatatc aaaaaaagta
      121 agatgactgt gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct
      181 tacggacgtg ggggttcaag tcccccccct cgcaccaatt atctttactt cctttcttgt
      241 ttcttccctg ttttgtcatc cctgattcaa attcattatc atcagcatga tgccagcaat
      301 aagcgccagg cacgatggta gcaaaatgta atgccgcaac tgtttgtgat acagcatcac
      361 cggcgtcaat actaatccca tcagaatgac aatccgccac tgatccggcg ataaatccgc
      421 atacatcagc cctgcaatca gcaatcccgg cagcaacact ccccagccac tgcccagcgt
      481 acgttgcaac atggtttcat ctctttcatt gataatgaaa accaatatca tatgataatt
      541 tttatcattt gcaagccaga gaaatccctt gctatcgagt aaacctatcg ctaggattag
      601 caatcattat catttagatt actatcacga ttatggccta tcgttccgca ccgctctatg
      661 aagatgtcat ctggcgaacg catctccagc cgcaggatgc tgggcttgca caagccgtac
      721 gggcgatgat cgccaaacat cgtgaacatt tgctggagtt tatccgcctg gatgaacccg
      781 ctccgcttaa cgccatgacg ctggcgcaat ggtcatcacc gaatgcgcta agttctctac
      841 tggcggtcta ttccgatcat atctatcgca accaaccaac gatgatccgc gagaacaaac
      901 cgctgatctc actatgggca caatggtata tcggcctgat ggtgccacca ttaatgctgg
      961 cgctactgac gcaggaaaaa gcattagatg tgtcgccgga acatttccat gctgagtttc
     1021 acgaaaccgg acgcgtcgcc tgtttctggg tcgatgtgtg tgaagataaa aacgcaacac
     1081 cacattcgcc gcagcagcga atggaaacgt taatcagcca ggcgctggtt ccggttgtgc
     1141 aagcactaga agcgaccgga gagatcaacg gcaaacttat ctggagtaat accggttatt
     1201 tgatcaactg gtatctcact gagatgaagc aactgctcgg cgaggctact gttgaatcgc
     1261 tgcgccatgc cctctttttt gagaaaacgc tcacgaacgg tgaagataat ccactctggc
     1321 gtaccgtggt actgcgcgac ggcctgctgg tgcgccgcac ttgctgccag cgttatcgct
     1381 taccggatgt gcagcaatgt ggctattgta cgctgaaatg aaagcactgc cggggaagta
     1441 aacccggcat catgcggatt aatagggatg caacacatta cttgcttcgc aatgccgcat
     1501 acataacaga atatcttcgg cctccaacaa ccctgcatca gaactgacgc ccagtttcga
     1561 catcacatta aatttatgcg ctcggatcgt tttgatatta cgtttaagct gctcggcaat
     1621 ttgtgtcatt gagtagccac gcgacataaa gcgcaatatt tctctctccg tcgggcttaa
     1681 cgtccggctt tggttaatgt accactgatt gttcagtcgg tcggtcgcct gacgtacacc
     1741 atttaatgac aaaaagagtt cctgatgaaa aatctccagc gttgacgctt tacttaatac
     1801 accgtccagc ggtgatggcg acaatgaacc aatcagccga gcttcaatat catcatccac
     1861 aataactaaa cgccgggtac gcggaaactt aatcgccagc tcagtcaggc aagataatcc
     1921 ctctctgcgc tcacttctca tggcagaaaa agaaaaaatg accgccgcaa acgagatccg
     1981 cgacatggca tcct
//
LOCUS       DAJTRM010000234         1960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRM010000234 DAJTRM010000000
VERSION     DAJTRM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.240"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..160)
                     /gene="ospE1"
                     /locus_tag="OO322_004192"
     CDS             complement(<1..160)
                     /gene="ospE1"
                     /locus_tag="OO322_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR5908811.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAA"
     gene            complement(342..743)
                     /locus_tag="OO322_004193"
                     /pseudo
     CDS             complement(342..743)
                     /locus_tag="OO322_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1172..1960
                     /gene="virF"
                     /locus_tag="OO322_004194"
     CDS             1172..1960
                     /gene="virF"
                     /locus_tag="OO322_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5908812.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          611 a          344 c          368 g          637 t
ORIGIN      
        1 tggcagcagg tatttgtaat tcgtatatag ctgctttttt attgaaaaca gtatatccat
       61 ctgtagtaac agtagaagat aataatacag gatttgatac ttcaaggatg atattttctt
      121 gtttttgtgg aaggcaaggg aatatagttt gtgtaagcat attgcttcct tatgttatat
      181 tttagtaaag aatttatttt ttacattata gtcggctaaa tgaagtttac tgtaaaaaaa
      241 cgatactttt ttcgtaagcg atgagatgtg gagccagaaa aatggctgcg ttacgtcatt
      301 gaactgtcct tgacccacga tattcagaca gtcgttgtat cttaagttga cattacccgc
      361 tgacgtcgat attcccttgg tgagagataa cccaacgcac tatgcggatg gttttcattg
      421 taatgctcga tcgccactgc aaggttatgc aatgccgttc ttggtttcgg catgaacgcg
      481 atgcagtctt ccttcatcgt tttcatgaac cgttctgcta ttccattact ctgagggcta
      541 ctgactgccg tcgtgcaggg ctccagattc aactctctgg cgaactgccg tgtttcatga
      601 gccgtataag ccgaaccgtt atcggccact ggattggccg ttccggtcct ttatcgccat
      661 atcgctcctg acatgacatc ctgcaccgtt gctttgtcat aatctcccgt gcctgctgcc
      721 cagtctatgg cttcacgatc gcaatatgga acgtaacgct gtcatcggga gaatcgtcaa
      781 tgcggcattc ggttaacgct taccaatatc aacaccaacc ggatttgtgg agcctccagt
      841 ctgaaggggc tttatgcgtt ccgtatagga tattatgatg ctggagtttt gcgaagctcc
      901 actcctttcc gaacatttac acagatattg cctaagaaaa gtgaaaccat aatatggtta
      961 tagtcccttt cagtgcaaat acttagcttg ttgcacagag aaatagaagc tgcataagct
     1021 ctttcttcaa aaaatgtaaa taaagttaaa tataggaaaa attacttaat ctatcttaat
     1081 aacggaaaga ttttgtatac aatcactgtt acagaaattt cttagttact ctgtaaacac
     1141 taaatatagt ttggtatatt ctgttgaatt tatgatggat atgggacata aaaacaaaat
     1201 agatataaag gttcgcttgc ataactatat tattttatat gcaaaaaggt gttcaatgac
     1261 ggttagctca ggcaatgaaa ctttgactat cgatgaaggg caaattgctt ttatagagcg
     1321 aaatatacaa ataaacgtct ccataaaaaa atctgatagc attaatccat ttgagattat
     1381 aagccttgac agaaatttat tattaagcat tattagaata atggaaccaa tttattcatt
     1441 tcaacactcc tattctgagg agaaaagggg gttaaacaaa aaaatattcc tcctctctga
     1501 ggaggaggtt tctatcgatt tgttcaaatc tataaaagag atgcctttcg gcaaaagaaa
     1561 gatctatagt ttagcttgcc ttttatcagc tgtttctgat gaggaagctt tatatacttc
     1621 gatatcgata gcttcttctc ttagtttttc tgatcagata aggaagattg ttgaaaaaaa
     1681 catcgagaag agatggcgtc tttctgatat ttcaaataac ttgaatttat cagaaatagc
     1741 tgttagaaaa cgattggaga gtgaaaaatt aacatttcaa caaatccttc ttgatattcg
     1801 catgcatcat gcagcaaagc ttttattgaa tagtcaaagc tatattaatg atgtatcaag
     1861 acttatcgga atatcaagcc catcttattt tataaggaaa tttaatgaat attatggtat
     1921 aactccaaag aaattttact tatatcataa aaaattttaa
//
LOCUS       DAJTRM010000235         1865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRM010000235 DAJTRM010000000
VERSION     DAJTRM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.241"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..1865
                     /gene="sat"
                     /locus_tag="OO322_004195"
                     /pseudo
     CDS             1..1865
                     /gene="sat"
                     /locus_tag="OO322_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          694 a          322 c          363 g          486 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaa
//
LOCUS       DAJTRM010000236         1846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRM010000236 DAJTRM010000000
VERSION     DAJTRM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.242"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..163)
                     /locus_tag="OO322_004196"
     CDS             complement(<1..163)
                     /locus_tag="OO322_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR5908813.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGIS"
     gene            complement(176..1465)
                     /gene="arsB"
                     /locus_tag="OO322_004197"
     CDS             complement(176..1465)
                     /gene="arsB"
                     /locus_tag="OO322_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5908814.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1519..>1846)
                     /gene="arsR"
                     /locus_tag="OO322_004198"
     CDS             complement(1519..>1846)
                     /gene="arsR"
                     /locus_tag="OO322_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5908815.1"
                     /translation="FKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHLAL
                     LRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCSGD
                     SKNICS"
BASE COUNT          501 a          510 c          466 g          369 t
ORIGIN      
        1 cggaaatccc catatcggca atgagtttga ccagttcatc gcgcgttggc ggagtttcca
       61 gataatggat aatggtcggt tctgtgccgc tgttgcggat catctccagc gtattacgcg
      121 acgtgccgca ggccgggttg tgataaatgg taatgttgct catatcagta tctcattaca
      181 aagtgaaaga gagacgtagc gccagcgcag ccagcgtcac aaacagcaca ggcagggtca
      241 tgataatccc tgtacggaag taatatcccc agctgatagt catattcttc tgcgaaagta
      301 cgtgcagcca gagcagcgtt gccaggctac caatcggggt aattttcggc cccaaatcgc
      361 agccaatcac gttggcataa accatcgctt ctttgataac gccagatgcc gtgctgccat
      421 caatggacaa cgcgccaacc agtaccgtcg gcatattgtt cataatagaa gagaggaagg
      481 cggtgaggaa tccggtgccg agcgtcgcgg cccacaggcc gttatccgcc agcacgttga
      541 gtacgccaga aagatattcc gttaatccgg cattgcgcag gccataaacc accagataca
      601 tgccgagcga gaagatgaca atctgccagg gggcaccgcg caggacttta cccgtattaa
      661 tcgcatgacc gcgtttagcg acgacaaata atatcagtgc gcccacagct gcaatggcgc
      721 tcaccggaat gccgagcggt tccaggacga aaaatcccac cagcagaagc agtaaaacaa
      781 cccagccagt tttgaacgta gcaggatctt tgatcgcttc tgcgggagat ttcagcagcg
      841 ccatatcgta gttctgcgga atatctttgc gaaaatagag atgtagcatc accagcgtgg
      901 caacaatcgc ggcgatatcc accggcacca tcaccgaggc gtattcgcga aagccgaggc
      961 caaagaaatc agcggaaacg atattcacca ggttggagac aataagcggc aggctggcgg
     1021 tatcggcaat gaatccggcc gccatcacga acgccagcgt cgtgccttta ctgaatccta
     1081 aagccagcag catggcgatg acaatcggcg tcaaaataag cgccgcgcca tcattggcaa
     1141 acagggccgc aacggcggca ccgagcagga caatccaggt aaacagcaag cgaccgcgtc
     1201 cattgcccca gcgtgaaacg tgcagcgctg cccattcaaa aaagccggac tcatccagca
     1261 gcaggctgat gataatgacg gcaataaacg ccgccgtcgc gttccagacg atattccaca
     1321 ccaccggaat atcacccgga tgaaccacgc ccgtaaccaa cgccagtacc gcgccgagcg
     1381 ttgcactcca gccgatgcct aaacctttcg gctgccagat aaccaatacg atggtcagga
     1441 caaagatagc gcctgccagt aacataatgc ctcccggata aaacacatgt gcaaatccat
     1501 atgtgtttag ctaaattttt aactgcaaat gttcttactg tctccggaac agttttgtct
     1561 ggccaggttg cggacaattg cctgaatctt ttcctgttca catcgccagg cttcatcaat
     1621 aattttcgcc gcccatgctg gaatatgcgg cgataagcgg taatgaaccc acttaccttg
     1681 cttgcggtcc agcaatagcc cgctttcacg cagcaatgcc agatggcggg atatcttggg
     1741 ctgcgactga tcgagggcag tgcagagatc gcagacgcat aactctccca gttcgctaag
     1801 cagtaaaacg atgcccagac gggtttcatc agcaagaatt ttgaac
//
LOCUS       DAJTRM010000237         1834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRM010000237 DAJTRM010000000
VERSION     DAJTRM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.243"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            32..211
                     /locus_tag="OO322_004199"
     CDS             32..211
                     /locus_tag="OO322_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908816.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            263..457
                     /locus_tag="OO322_004200"
     CDS             263..457
                     /locus_tag="OO322_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908817.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1256..1592)
                     /locus_tag="OO322_004201"
                     /pseudo
     CDS             complement(1256..1592)
                     /locus_tag="OO322_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          569 a          397 c          374 g          494 t
ORIGIN      
        1 actacagcgc attgctttac gagtaataac aatggattta tcaccagaag acgcaaagtt
       61 tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc aaacgggcat
      121 gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg acgacaattc
      181 caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac agtggtgtga
      241 tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg agcaactgtt
      301 acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc cacatgaacg
      361 cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa tcaggtctga
      421 taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat attgccacca
      481 accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac tgacgagctt
      541 gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac ggcgtacaag
      601 cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt gtcatacact
      661 cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc gaaatccatc
      721 tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat ctgcgcccaa
      781 tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg ttacagagtt
      841 ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta accgggaaat
      901 tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg atgctatacg
      961 atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc agggttggca
     1021 atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca aaatatgccg
     1081 cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac actgctaaaa
     1141 cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca gaatctgcat
     1201 tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac ttacactata
     1261 cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta gttgtcacaa
     1321 tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt aattctgcgt
     1381 tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca gttccccttt
     1441 ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc ctggattata
     1501 gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc agatccagtc
     1561 cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat tgagtcgaaa
     1621 acaagcacta ctctatacag gataattaga ggtcagtttg attattcaca attcatcatc
     1681 agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata attattataa
     1741 taacggcttt aattggaata catatattac aacgtattat atataattgg tattctggga
     1801 actatattct caaaatacag tagaaacgag gtat
//
LOCUS       DAJTRM010000238         1731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRM010000238 DAJTRM010000000
VERSION     DAJTRM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.244"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1520)
                     /locus_tag="OO322_004202"
     CDS             complement(<1..1520)
                     /locus_tag="OO322_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5908818.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHK"
BASE COUNT          400 a          455 c          450 g          426 t
ORIGIN      
        1 attttgtgct gttccagata gaactgattc accgttttca gccagctgcg cgcgatttca
       61 tcacccagaa ggtcatcgcc atacatttta aatccctgaa ttgccatcca ttgtaacggt
      121 gcccagccgt tgggtttatc ccactgttca ccggtttcgt actcgcttgc caaaatcccg
      181 ccaggtgtca gtaaccggct gcgtacggcg tttgccagac gatcggcctg ttcatggttc
      241 gccatgccga catagagcgg cacaatggcg gcagcggaaa acagcgccag ttgttcgcgt
      301 cgccagtcgt aatcgcggta gatgccgttt tcatcatccc agaggtaacg gtttaccgca
      361 tcgcgacggg cactggcctt ctggcggaac agcgcttctg tctctttctc gcctttcagc
      421 gccgagatgt tggcgatggc gctctccagt ttaaacagga aggcattcag atcgatgggg
      481 atgaactggg tggtacgaat gctcgccaga cgaccagtat cacgcagcca acgggaagag
      541 taatcccaac cggaggccgc tcccgcgcgt aaatcgcggt acacctcgtt gggcgggcga
      601 ccagaatgtt tcgcggtttc aacgtcctca agccaggatt cgtcacgcgg cgtgtcgcga
      661 tcgtcccagt aacggttgag cagcgatccg tccggcatcc gcacaacatg gcgataggcc
      721 tgattaggga tcaacgattc tgcaccgtcc atccagaagg catattccat tttaaggtgg
      781 tcgagatagc ggcgcgcacc gcgtacacca tcttcttcaa acaactccac catcagcgca
      841 aaaaccggtg gttgcgagcg gctcaaataa taggtgcggt tgccgttggg gatgtgaccg
      901 tagttttcga tcatccaggc gaagttatcg gccatgcatt tcagcaaatc ttcccgacca
      961 ctttccgcca gtcccagcat ggtgaaatag gaatcccaat agtaggtttc gctaaaacgg
     1021 ccgcccggga caatatatga ctgcggtagc gccagcagag aagaccacgg aatgtgatcc
     1081 tgtggttcgc gggttagcac cggccacagc tggtcgatat gctctttcag ggaattttgc
     1141 gggtccgata catactcgct ggagtagacc tccggcagcc agaagtgatt ttcaacaaac
     1201 ttgcgcaagt caaaatcacg atgacggcgc actttacggt agcggattaa aatatccagc
     1261 gggtccattt tgggtgcgca gtcgggaaag gttttactgt cggggaaaat tttcgccgac
     1321 tgaacatgct cgaacagttc gagatagcga tcggcaggcg tcagcgcatc agaggcgggc
     1381 agcccttcaa tcatctcggg ttccggttct gcctcgatca tctcatccag ttttaattca
     1441 tacgggtcca gttcatagca gagatcgacc tcgatcatca gttcgtctgg attagggttt
     1501 tgaattttct gattgagcat aaggatccaa cctccgaaat tcgtcgctaa ttatcgggct
     1561 tttctttaag ggtagtcatt cgctccgcct ggtgggcgac aaagcgtgcg gtggatcacg
     1621 tttattaact tgtcaaaaaa tataacgcac taatatcaat ggaatttata aaaatacgga
     1681 tttggtgttg tttttcattt tcagaacatt caatcctgct ctcgccgcaa t
//
LOCUS       DAJTRM010000239         1721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRM010000239 DAJTRM010000000
VERSION     DAJTRM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.245"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..364)
                     /locus_tag="OO322_004203"
     CDS             complement(<1..364)
                     /locus_tag="OO322_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5908819.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..1645)
                     /locus_tag="OO322_004204"
     CDS             complement(431..1645)
                     /locus_tag="OO322_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5908820.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          497 a          452 c          366 g          406 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtacgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      961 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
     1021 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
     1081 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
     1141 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
     1201 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
     1261 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
     1321 gcagttgata caatggcata tttgccgctt tagctttgat atcccacaac gccatatcaa
     1381 tggcagaaat ggctgacatc gtgacggggc cacgtcgcca gtaagcacct ttataaaaga
     1441 attgccagat atcttcgata cggctggcat cgcggccaat tagttgtgga caaagatgat
     1501 ctttcagata agaagcgacc gacaattcgc gaccatttaa cgtggcatct cctaatccac
     1561 aaagtccgct ttcagtggtt attttaagcg taacgaaatt acgccctggg caagtaacaa
     1621 aaacatctgc agcaataatt ttcattattg caatctcctg tgttgtaaat agacgtttca
     1681 aacctaattt tacgcctact accatacaag agagaaaaag c
//
LOCUS       DAJTRM010000240         1711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRM010000240 DAJTRM010000000
VERSION     DAJTRM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.246"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..597
                     /locus_tag="OO322_004205"
     CDS             <1..597
                     /locus_tag="OO322_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5908821.1"
                     /translation="AQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISFI
                     GVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTSL
                     ADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGIF
                     ATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            642..872
                     /locus_tag="OO322_004206"
     CDS             642..872
                     /locus_tag="OO322_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908822.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            850..1350
                     /locus_tag="OO322_004207"
     CDS             850..1350
                     /locus_tag="OO322_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5908823.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1350..>1711
                     /locus_tag="OO322_004208"
     CDS             1350..>1711
                     /locus_tag="OO322_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908824.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTV"
BASE COUNT          448 a          402 c          456 g          405 t
ORIGIN      
        1 gcgcagaagt ttaagccgca ggcatacacc cttgataccg atggtaacaa agagaacgtc
       61 gacaaccgtg ttgcaacggt gaccgtatcc tccaccaccg gatttaagcg cggcgacaaa
      121 atcagcttca ttggtgtgaa gttcctgtct cagatggcga agaatgtgct gactgatgac
      181 gcgactttct caatcacccg tgtgatcgat ggtactcaca tcgaaatcac gccgaagccg
      241 attgcgctgg atgacgcgtc actgacaaaa gaagagaagg cttacgctaa cgtaaacacc
      301 tctcttgctg ataccactcc ggtaaacgtt ctgaacgtgg caacaaccac cgctaacgtg
      361 ttctgggctg atgactcaat ccgcctgctg tctcagccga tcccggtaac ccatgaactg
      421 tttgctggca tgaaaacgtc ttccttcagc attccaggta ttggtgttaa cggcatcttc
      481 gcaacgcagg gtgatatcaa cactctgtct ggtaagtgcc gtattgctgt gtggtattca
      541 gcatgtgctg tacgaccaga ggcaattggt gttggtctgc ctaaccagac cgcgtgataa
      601 ccagagggag cttcggctcc cgtttttatc tggagacaag catgacacac atgatctttc
      661 gtcatggcga catgaagaag tggaaaggcg ttggctacga ctttgaaatc gtgaaagccg
      721 aagagcttca ggaatatctg gatgctggct ggttttcaca tcctgatgac cttttgaagg
      781 atgttgcaga gccagagcca gaagaaaaac agcgtaaaaa gcctggtcga aaacctaagg
      841 cggcagcaga tgaacctgac aacgaagggt gatttagttc ttgcggcatt acgtaagctc
      901 ggtgttgcat caaatgccac gttaaccgat gtcgaaccgc agtctatgga agacggcgtc
      961 aacgaccttg aaatgatgat ggctgaatgg cttggcggtg atgcgtcacc tggtatcaac
     1021 gttggctaca tttttgctga tgcagatgtt gctccggatc cgggcgatga gcacggttta
     1081 tcaaataacg ctatcaatgc cgtcattttc aaccttgcct gccgaattgc tccagattat
     1141 gcgctggaag cgtctgcaaa acttataacc actgccagat acgggaaaga gcgactcgtc
     1201 aaactgtctg caatggacag ggcaaaagca gctaaatgta agtccggtta tccaaaccgt
     1261 atgcctgttg gtagtggtaa ccagttggcg aagtggaatg gttggaatta cttccaccga
     1321 aaggaacctt gcgataacgg gagcgaataa tgcctattca gcaacttccg cttatgaaag
     1381 gtgtcggcaa agacttccga aacgccgact atatcgacta tctgccagtg aatatgctgg
     1441 ctacacccaa agaaatcctg aacagcagcg gatatcttcg ctcattcccg ggcattgcca
     1501 aacgttctga cgtgaacggt gtatctcgcg gcgtcgagta caacatggcg cagaatgctg
     1561 tttatcgcgt gtgtggcggc aagctgtaca aaggagaaag tgaagtcggt gatgttgccg
     1621 gaagtggtcg tgtatcaatg gcgcatggtc ggacatcaca ggcggtaggc gttaatggtc
     1681 aactggtcga gtatcgctat gatggcacgg t
//
LOCUS       DAJTRM010000241         1673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRM010000241 DAJTRM010000000
VERSION     DAJTRM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.247"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(8..541)
                     /locus_tag="OO322_004209"
     CDS             complement(8..541)
                     /locus_tag="OO322_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5908825.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(607..1236)
                     /locus_tag="OO322_004210"
     CDS             complement(607..1236)
                     /locus_tag="OO322_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908826.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1240..1455)
                     /locus_tag="OO322_004211"
     CDS             complement(1240..1455)
                     /locus_tag="OO322_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5908827.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          443 a          448 c          350 g          432 t
ORIGIN      
        1 actctgctca ttgatctatc ccccaacagg ctaatgcgct ttcctggtca cgacgaataa
       61 cctgtccata gcagttattt gaacgtatgc ggcaatcgcg cccaacatct tttatccacc
      121 agcgaatcgc ctcgcatgcg cccttacgat caccggcatt cagccgctta taaaacgtcg
      181 acgggaaaca cttaccgggg ccaatgttat aggggcaaaa tgacgctata cccgctttct
      241 ggggttcggt cagaggtact ttaatattgc gctccaccca tgccagcgcc ttatcacgtt
      301 caatagcgtt aacctggtca catttttcct tcgacagctt cattcccggt atgacaggct
      361 taccatccac cattgtggca ccacgacaga tggtccatat gccagaacca tcacggtatg
      421 ccgttgtgtg attaccctct ttttcgttca gaaactggtc aagtatctgg ggagcagacg
      481 cgcctgcggc aatcagcgcc agaacagcag ccgacaggcc gtatttgatt tttgcgctca
      541 tggatattta tcaggattta tcggcaacaa ataacgagcc aacttatata cgccttttaa
      601 gataagtcag ccccggctgg atccagtcat caggctcttt cttaaagggg gagtattgaa
      661 atcacgaaga agagcctccc gcaccgcagc atccatgtct gcaccactgg caagtgcttc
      721 aatctctgcc gccacctgca aataccccat gcaacggcca atgcgtttca taactctggt
      781 gttttcgtct ttcaggcatc tgatagcgag attaataatt ttcaggcagt gatgatgcga
      841 tgcccggaca ggttttccgt tctcataaac gacgaactca ccctcttcac aggaaaccct
      901 gaaaggccga tggggttcct ggtctttatc cggtgcagac gcaataaaat cacttttatg
      961 ctcttcctct ccggcactgt taaccgtaat gacgtactca ggccgcaggt aagccgcctc
     1021 tttttctgac aatgcggccg gtaaaaactt ttcctgcaaa aacttcataa aagtctcgtg
     1081 agcgactttg tggcagtaat cgtaaatcgc cgcataatgc tcatcgcggc gtttgttttc
     1141 atcttcagaa ctcaccaacg ccgacaattt tttattaagc tcagtgattt cattttccag
     1201 gtgattgaat cgctgattca tttcttcatg cttcattatt tactctcccc gtgcagcctt
     1261 acgcttgtct tctcttattt tgaaatacag attcgtcaga taagtcagaa gccccagaag
     1321 cagacttccc agcacaccaa tcgcagccca ctgtgatggc ctgacctgat ccaaccactg
     1381 caaaaaccag tagccggcac tgccggcgga ggtgccgtag gcaatgcccg ttgaaatttt
     1441 gtccatggat ttcatagcct cacctccgca aataacggat ggcgtagttt tacactgaga
     1501 aatgaaaggg atttgaaaag aaaaaaacgc aaaagcgggc gaaacgatat atacagtaag
     1561 gaaagcactc tatccaacaa accacccaca gttaatcgga ataaaagcag agtgcttatg
     1621 aatgatcgca cgcccgaagg ttagtatttc tgcacagcaa ttttgcaaaa aaa
//
LOCUS       DAJTRM010000242         1669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRM010000242 DAJTRM010000000
VERSION     DAJTRM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.248"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..269
                     /locus_tag="OO322_004212"
     CDS             <1..269
                     /locus_tag="OO322_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5908828.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO322_004213"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO322_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5908829.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>1669
                     /locus_tag="OO322_004214"
     CDS             613..>1669
                     /locus_tag="OO322_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5908830.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPEFPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGC"
BASE COUNT          443 a          426 c          465 g          335 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac ccgatcgcca gtaagcctga attcccggga
      961 cgaaaatctt cacgaaaacc gctgccggca gaacttcccc gggagacaca tcgccttctg
     1021 cctgctgaaa ccagttgccc ggcctgtgga ggtgttctga aagaaatggg ggaaacaatc
     1081 tcagagcaac tggatatcat taataccgcc tttaaagtta tcgaaaccat acgtcccaaa
     1141 ctggcctgta gccggtgtga tgtcatcgtt caggcaccac ttcctcctaa accgatcgaa
     1201 cgcggttatg ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat
     1261 atccctttat atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat
     1321 accatggtgc gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg
     1381 aatgactatg ttctggaggc aggaaaggtg cacgcagatg acactccggt gaaagtactg
     1441 gccccgggga acggaaagac gaaaacgggt cgtctgtggg tatacgtcag ggatgatcgt
     1501 aatgcgggtt catccctgcc ggcagccgtc tggttcgcgt attcggcaga tcgcaaagga
     1561 gaacatccgc agctccacct ggcaaagtat cagggcgtac tgcaggctga tgcctatgca
     1621 ggttataacg tactgtacga aacgggccgg gtgaaggaag ccgggtgcc
//
LOCUS       DAJTRM010000243         1603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRM010000243 DAJTRM010000000
VERSION     DAJTRM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.249"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            240..509
                     /locus_tag="OO322_004215"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO322_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO322_004216"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO322_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO322_004217"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO322_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5908831.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          476 a          330 c          377 g          420 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaa
//
LOCUS       DAJTRM010000244         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTRM010000244 DAJTRM010000000
VERSION     DAJTRM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.250"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..360
                     /locus_tag="OO322_004218"
     CDS             <1..360
                     /locus_tag="OO322_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5908832.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO322_004219"
     CDS             361..726
                     /locus_tag="OO322_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5908833.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO322_004220"
     CDS             723..1406
                     /locus_tag="OO322_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5908834.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          452 a          324 c          438 g          386 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJTRM010000245         1577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRM010000245 DAJTRM010000000
VERSION     DAJTRM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.251"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..274)
                     /locus_tag="OO322_004221"
     CDS             complement(<1..274)
                     /locus_tag="OO322_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5908835.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAF"
     gene            complement(271..>1577)
                     /gene="btsS"
                     /locus_tag="OO322_004222"
     CDS             complement(271..>1577)
                     /gene="btsS"
                     /locus_tag="OO322_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5908836.1"
                     /translation="LIRRGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRL
                     VSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNE
                     VNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEV
                     VYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGE
                     GIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQAS
                     QLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQ
                     QLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASG
                     LGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          387 a          423 c          372 g          395 t
ORIGIN      
        1 caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt
       61 ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa
      121 acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact
      181 ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta
      241 acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa
      301 cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt catcgccaaa
      361 ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg
      421 ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc
      481 gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc
      541 cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc
      601 aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac
      661 atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa
      721 aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc
      781 tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt gaagcagttt
      841 gatttctgac tgggtgagca tcgctttttg ccgctcatat tgccctgcaa gaatctgcgc
      901 cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga ataaccggtt
      961 tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac gcaacggaat
     1021 taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt
     1081 gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat
     1141 cggtttgccg ggtaaatggt ggtcgtcacc aattccggta aaggccagca atttctctcg
     1201 atcggtaatc gcgactgcac caatatccag ctcctgatac agtacctgag ccactttcat
     1261 gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag
     1321 cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat
     1381 acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag caatattact
     1441 caccagacgc accgcatctt cataaggtcg ggcgatcgca aggatgatca gcatttgcac
     1501 catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg
     1561 cccgcgacgg atcagga
//
LOCUS       DAJTRM010000246         1551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRM010000246 DAJTRM010000000
VERSION     DAJTRM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.252"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            400..1149
                     /locus_tag="OO322_004223"
     CDS             400..1149
                     /locus_tag="OO322_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5908837.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1551)
                     /locus_tag="OO322_004224"
     CDS             complement(1319..>1551)
                     /locus_tag="OO322_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908838.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          425 a          329 c          315 g          482 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta t
//
LOCUS       DAJTRM010000247         1490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRM010000247 DAJTRM010000000
VERSION     DAJTRM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.253"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..99)
                     /locus_tag="OO322_004225"
     CDS             complement(<1..99)
                     /locus_tag="OO322_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5908839.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQS"
     gene            237..1157
                     /locus_tag="OO322_004226"
     CDS             237..1157
                     /locus_tag="OO322_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5908840.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1157..1490
                     /locus_tag="OO322_004227"
                     /pseudo
     CDS             1157..1490
                     /locus_tag="OO322_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          415 a          346 c          357 g          372 t
ORIGIN      
        1 tgactgcacc atgctcaggc gggctgctgc gcgagtgaag ctacgctcct ctgcaacgac
       61 gacaaacgcc atcagatcag cgatttcttc acgcttcatt cagtatctcc atacaatcta
      121 ttaatcattt aaatgatagg tctaatcatg atttagggtc taatcaactt aatgatcaat
      181 atctatagtg atatcaccaa ccgtcaaaac gaaccgacaa ctccatgagg aactgaatga
      241 tgaataataa agtcagcttc actaacagca ataatccaac catctctttg tctgcagtga
      301 tctatttccc tccaaaattt gatgaaaccc gtcagtatcc ggcaattgtg gtatctcacc
      361 caggtggggg cgttaaagag caaacggccg gaacctatgc cgaaaagctg gcggaaaagg
      421 gatttgtcac tattgcttat gacgcatctt atcaaggtga aagtggcggc gagccgcgtc
      481 agttagaaaa cccatacatt cgtactgaag acgtcagtgc cgtgattgat tacctgacaa
      541 cgctttctta tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc ggtgcaggat
      601 atacagccaa tgctgcaatt caggatcgcc gcatcaaggc catcggtacc gttagtgcgg
      661 tcaatattgg ttcgatgttc cgcaatggtt gggaaaacaa tgtgaaatct attgatgctc
      721 tgccacacat tgaagctggc tcaaacgccc gaaccagcga tattagcagc gaagaatacg
      781 ccattatgcc actggctcca atgaaggagg ccgatgcgcc gaacgaagaa ctgcgtcagg
      841 cctgggagta ttaccacaca cctcgtgcgc agtatccaac agcaccgggt tacgctactt
      901 tgcgcagcct taaccagatt attacctatg atgcttacca tatggcggaa gtgtacctga
      961 ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg agtgacgacc
     1021 tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc gcaaaccata
     1081 tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta gctccgttct
     1141 ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg cctggcatat
     1201 tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc cggcgattat
     1261 cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct atggcaaggc
     1321 attggctgaa aaaggctatg tcgttctcgc ttatgacgcc agtttccagg gagaatctgg
     1381 tgggtagcct cgttggatag aagatccgac acagcgcgta gaggatatta gccgtgttat
     1441 cgattatgca gtaactctgc catatgttga tgctgaacgc attggcgtgc
//
LOCUS       DAJTRM010000248         1459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRM010000248 DAJTRM010000000
VERSION     DAJTRM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.254"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO322_004228"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO322_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5908841.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="OO322_004229"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="OO322_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1459)
                     /locus_tag="OO322_004230"
                     /pseudo
     CDS             complement(1294..1459)
                     /locus_tag="OO322_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          450 a          224 c          318 g          467 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcct
//
LOCUS       DAJTRM010000249         1449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRM010000249 DAJTRM010000000
VERSION     DAJTRM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.255"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            88..1281
                     /locus_tag="OO322_004231"
     CDS             88..1281
                     /locus_tag="OO322_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5908842.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1301..1449)
                     /locus_tag="OO322_004232"
                     /pseudo
     CDS             complement(1301..1449)
                     /locus_tag="OO322_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          424 a          315 c          342 g          368 t
ORIGIN      
        1 aagggctgag gaatccccag aaaacgagac aaacatagca ttatgtgatc tactgatttg
       61 tgccaacaat tcaatgagat cactgctatg cctgttcata aagtatgtca gaatttcttc
      121 cgggatgcct tagcaccatt ccatcagtac cgacaaaatg ccctgatgga tgccacaatg
      181 gcgttaatca acggagcatc tctgacgcag accagtatcg gacgtttttt gccgggtaat
      241 gcacaagtta agaataagat caaacgtatt gatcgcctca tgggtaatga ggcgctccat
      301 cgcgatattc caatgatttt caggaatatc acttcaatgc tgacacggca gttatcttta
      361 tgtgtaatag ccgtagactg gagtggctat ccctcccagg agcatcatgt tcttcgtgcc
      421 agccttctgt gtgacgggcg ttccattccg ttattgagta aggttgttcc ttctgaaaaa
      481 caaaataatc cattgataca acatgatttc cttgattccc ttgctcaatc cctgcccccc
      541 gatgccaggg ttattattgt cacggatgct ggatttcaga gcgcatggtt tcaccatatt
      601 acttctctgg gctgggattt tattggtcgg atcagaaata acgttcagta ttgcctcgat
      661 aacgcgcctg aaagatggtt aaaagtgtcg gatagccctg aatgtaaaac accggaatat
      721 atgggggctg gaaggctggt taaagaaagg aaaaaaagca tccgggggca tttttacacc
      781 tataagaaat cggcaaaagg gcgaaaaaag aaacgttcga aaggtcagag tggtttaaac
      841 aaaacagaca aagaacagag caaatcagca aaagaagcct ggttgatctt cagcagtaca
      901 aatgacttca gagcgcgaga aatcatcaag ttatacagtc ggcgaatgca gatagagcaa
      961 aactttcgtg atgaaaagaa cgggcgcttc ggatttggtc ttcgggccag caaaagtcgt
     1021 tcaacaggaa gaattctggt tctgagtctg ctggcaacgt tgagcactat cgtaatgtgg
     1081 ttactcggtt atcacgctga aaataaaggg ttacacctga aatatcaggc taacagcatt
     1141 aaatcccggc gggttatctc atatctgacg ttagcgaaga atgttcttcg acactcgcca
     1201 cttattttaa gacgaacagt actgagcaca gttctaaacc atctgtccag aacctaccga
     1261 aatatggtgc tggtttatta gcagtaattt tgtggggatc cctcagcctg aaggggcgac
     1321 acagtcgggg agagaagccc atctccttgc cgaactgcca cagcgaagga tggaaccggt
     1381 gctgaccggt ctgatatgcg tcacgttgca gaaccacagt tttcatattg tcatacaaca
     1441 cttcgcgcg
//
LOCUS       DAJTRM010000250         1449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRM010000250 DAJTRM010000000
VERSION     DAJTRM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.256"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(100..567)
                     /locus_tag="OO322_004233"
     CDS             complement(100..567)
                     /locus_tag="OO322_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5908843.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(633..872)
                     /locus_tag="OO322_004234"
     CDS             complement(633..872)
                     /locus_tag="OO322_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5908844.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1007..>1449)
                     /locus_tag="OO322_004235"
     CDS             complement(1007..>1449)
                     /locus_tag="OO322_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5908845.1"
                     /translation="NEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKG
                     VRYLGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIK
                     HTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          467 a          324 c          255 g          403 t
ORIGIN      
        1 gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta ctgagagaaa
       61 aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca atgggaaaac
      121 aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac cgttttcacg
      181 aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc aaaaggtcat
      241 tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa cagttaacag
      301 cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca cgagcaaaac
      361 gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca aaaaaataag
      421 cacggttaac accagtaaat aacgatgact aataattctg agtaatatat ccccccatga
      481 aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc atattgctgc
      541 cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt tacgtctgac
      601 agcctctaga gagttttttt attttttata agttacggat tactcgttcc ggaaatcgtt
      661 acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc caggaagcct
      721 gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc aatcaatgca
      781 tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca ctgtttagtt
      841 ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat tatttaaatt
      901 caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat gtggctataa
      961 ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat ttatcttgat
     1021 taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct aacacggtaa
     1081 gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt ttgtcagtat
     1141 gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc tcaggaatgt
     1201 ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta ctgtcttcat
     1261 cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat ctgacgcctt
     1321 ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat gcctttctaa
     1381 gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc tcccgccaaa
     1441 cctcattga
//
LOCUS       DAJTRM010000251         1406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTRM010000251 DAJTRM010000000
VERSION     DAJTRM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.257"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..169
                     /locus_tag="OO322_004236"
     CDS             <1..169
                     /locus_tag="OO322_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5908846.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="OO322_004237"
     CDS             154..792
                     /locus_tag="OO322_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5908847.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..>1406
                     /locus_tag="OO322_004238"
     CDS             1020..>1406
                     /locus_tag="OO322_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908848.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          348 g          394 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagt
//
LOCUS       DAJTRM010000252         1372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRM010000252 DAJTRM010000000
VERSION     DAJTRM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.258"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(16..681)
                     /locus_tag="OO322_004239"
     CDS             complement(16..681)
                     /locus_tag="OO322_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5908849.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(850..1206)
                     /locus_tag="OO322_004240"
     CDS             complement(850..1206)
                     /locus_tag="OO322_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908850.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1264..>1372)
                     /locus_tag="OO322_004241"
     CDS             complement(1264..>1372)
                     /locus_tag="OO322_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5908851.1"
                     /translation="RYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          331 a          350 c          297 g          394 t
ORIGIN      
        1 aggtgcccca taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat
       61 aagagggcgt ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc
      121 acaatattcc tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc
      181 gccctgcttg cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg
      241 ccgcccacag tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct
      301 gatcacactg aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa
      361 acacatggtg caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa
      421 ccagttgtct gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg
      481 aatattctca tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa
      541 aaagatggtg tagtcgattt ccgaggcctg aagcgcctcc atcacctcac cggaacatgg
      601 agcacagcaa gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg
      661 tttgaaatca gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt
      721 agcattagaa cggggctatc ggaaacagat gtgtgactgc tccccgccct ttcgggcggt
      781 ctcctgatga tttgagggtg cagaaatccc tccggttaag gattaaattt ttaacagtgc
      841 taaatttaat tattcagttc tggattttgt cgccctgcgt atccgcgctt tcgcgttacg
      901 ctcaatctgt atcagctttt ctatatttct ccgcctttcc tgttcctcct ggcgcaatag
      961 ccttacatca tctgccagtc tggtttctct ttttgccaca gagagcatcc agtcaaacgg
     1021 ctccacaacc gcaccgcaga ttttacagcg gacctgacgc tctttttcgt caacccgtac
     1081 agaggcgtga tggcaatatg gtctttccga tggcttataa agaaaattaa cctgattacg
     1141 agggtcatcc tcttttatcg gaaataaaac gatattgctt aactcatctt ctggttttat
     1201 ttccatgctc ctctcctttg atgcgaatgc cagcggtaat tgaagcctga tagctaattt
     1261 cattcacagt atcgcctcct gaaaattacc ctgatagaaa gccagtacac gctgcatagc
     1321 ttcgctcttc cggcactcgc gacagattat attcaggcgc ctgtcgtagc gg
//
LOCUS       DAJTRM010000253         1340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRM010000253 DAJTRM010000000
VERSION     DAJTRM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.259"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..274
                     /locus_tag="OO322_004242"
                     /pseudo
     CDS             1..274
                     /locus_tag="OO322_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            767..>1340
                     /locus_tag="OO322_004243"
     CDS             767..>1340
                     /locus_tag="OO322_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5908852.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDR"
BASE COUNT          327 a          311 c          411 g          291 t
ORIGIN      
        1 gattcagggg gccagtctag atgccaacac aaaggcaact aataaagata cagataaaga
       61 tctaaaaaca gatctaaccc atcccaaacc cttcccttcc ggaagggagt ttcgggattt
      121 tgtggctgga gtgcttgagg ggagattatc tggcggtact gcagcggaat tttgtaattc
      181 tgcggtggtt gcgttgcagg ctgctggcct ggatgtctgt cgtgagtatc cggtgccaga
      241 gcgtggtgac ggttgcggag ggcggattga tatcgtcgtg actgacagga acggtgtccg
      301 gtgtgggatc gagcttgacc gaaattctcc gcgacagaaa tcactgctca aaatcggtgc
      361 tgttgaaacc gggatatgtg tcttgcggcg cagtgatatc gcaaggcaca ccgagcaggg
      421 aattctggtt atcggtgggg ctgttcgcca gaaaaaattt gacccgttgt cagttgatct
      481 gcccgactgg ttgtcagaaa cactctggca tgagtgggtc cagttcaggc aggcattgcg
      541 aaaaccgatt cgaacggagc tgggcgctaa cggggcgata cgggaactgg aaaaattccg
      601 tcagcagggt tttacacctg agcaggtgat tcgacacagc atcgccaatg aataccaggg
      661 tctgttcgcg ccgaaaggtg ttcggcctga gacgttgctc cgacaggtta acaccgtctc
      721 gttgccggac agtgcgatcc cgccaggctt cagggggtaa cagaccatga aaaatattgc
      781 gacaggaggc gttctggagc gtatccgcag actgacccca ccacatgtaa ccgccccatt
      841 cagaacggtt gcggagtggc gcgagtggca acttgctgaa ggccagaaac gttgcgagga
      901 gatcaaccgc ctgaatcgtc agttgcgggt ggaaaaaatc ctgaaccgct ccggcatcca
      961 gccgttgcac cggaagtgtt cgtttgcgaa ttaccgggcg cagaacgacg gtcagcgaca
     1021 tgcactgagt caggcgaaat ccatagctga cgaactgatg acaggctgta cgaattttgt
     1081 gttcagcggt aagcccggca ccggaaaaaa tcatcttgca gccgcgattg gcaaccggct
     1141 gatggcgaag gggcgtagcg tgattatcgt caccgtgtcc gatgtcatga gcgtgttgca
     1201 tgagagctac gacaacggca aatccggtga aaaattttta caggagcttt gtggtgttga
     1261 cctgctggtc ctggatgaaa ttggcatgca gcgggatacg aaaaacgagc aggtggtact
     1321 gaaccagatt gttgatcgcc
//
LOCUS       DAJTRM010000254         1322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRM010000254 DAJTRM010000000
VERSION     DAJTRM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.260"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO322_004244"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO322_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5908853.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSSLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          368 a          242 c          298 g          414 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag tgagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actatctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc agggccagta cctcgtcagt
     1321 ca
//
LOCUS       DAJTRM010000255         1310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRM010000255 DAJTRM010000000
VERSION     DAJTRM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.261"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..626)
                     /locus_tag="OO322_004245"
     CDS             complement(<1..626)
                     /locus_tag="OO322_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5908854.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1300)
                     /locus_tag="OO322_004246"
     CDS             complement(740..1300)
                     /locus_tag="OO322_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5908855.1"
                     /translation="MIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSDTADQ
                     IVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGT
                     PLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIA
                     RYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          355 a          304 c          280 g          371 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
//
LOCUS       DAJTRM010000256         1301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRM010000256 DAJTRM010000000
VERSION     DAJTRM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.262"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..519
                     /gene="curA"
                     /locus_tag="OO322_004247"
     CDS             <1..519
                     /gene="curA"
                     /locus_tag="OO322_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5908856.1"
                     /translation="GCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPK
                     GIDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKK
                     RIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKN
                     FGKVVIRVAGDD"
     gene            717..>1301
                     /gene="mcbR"
                     /locus_tag="OO322_004248"
     CDS             717..>1301
                     /gene="mcbR"
                     /locus_tag="OO322_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5908857.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAK"
BASE COUNT          374 a          270 c          331 g          326 t
ORIGIN      
        1 ggttgcagag tggtgggggt agccggtggc gcggaaaaat gccgccatgc taccgaggtg
       61 ttaggcttcg atgtttgcct tgatcaccac gcggacgatt ttgccgaaca actggcgaaa
      121 gcgtgcccaa aaggtattga tatctattat gaaaacgtgg gcggtaaggt atttgatgca
      181 gtgctaccat tacttaatac atctgcgcgc attcccgttt gtggcttagt gagcagctat
      241 aacgctacag agctaccacc cggtccggat cgtttacccc tgttgatggc aacggtgctg
      301 aaaaaacgta ttcgcttgca agggtttatt atcgctcagg attatggtca ccgcatccat
      361 gagtttcaga aggagatggg gcaatgggtg aaagaggata aaatccacta ccgcgaagat
      421 attactgatg gtttagagaa tgcgccacag acgtttatcg gcctgctgaa gggtaaaaac
      481 ttcggcaaag tggtgatccg cgtcgcgggt gatgattaaa gtttcaacgg cggcgtaagc
      541 cgccataaaa atccaggcaa aaagcttatt ttcagcttta attaactaac cacgtcattg
      601 ttctgctgct aataacaaca aaggtgggtg gtttatgatt attcacttta atacactagg
      661 tgaactcctt ctgccatgca ggcagggttg gacagaaaac acgcgaagga attaaaatgc
      721 ctggaacggg aaaaatgaaa catgtcagtt tgactctgca ggttgagaac gacctgaaac
      781 atcagcttag tattggcgca ttaaaacctg gcgcacgcct gattactaaa aatctggcgg
      841 agcaattagg tatgagtatt acacctgtgc gtgaagcatt attacgtctg gtttcggtga
      901 atgcgctttc tgtcgcacct gcacaagcat ttacagtacc ggaagtgagg caaagtcagt
      961 tagacgaaat caatcggatc cgctacgaac tggaattaat ggcagttgcc ctggctgttg
     1021 aaaacctctc ctcgcaagac atagcagaac ttcaggaatt gctcgggaaa ttacagcagg
     1081 cgcaagaaaa gggcgacatg gaacaaatca ttaatgtgaa caggctattt cgcttagcga
     1141 tttatcatcg ctcaaatatg cccatcctgt gtgagatgat tgagcaactg tggatcagga
     1201 tgggacctgc tttacattat ctttatgaag cgattaaccc agcggaatta cgggagcgca
     1261 tagaaaacta tcatctatta ctcgctgcgt taaaagcaaa a
//
LOCUS       DAJTRM010000257         1301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTRM010000257 DAJTRM010000000
VERSION     DAJTRM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.263"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..1037)
                     /locus_tag="OO322_004249"
     CDS             complement(<1..1037)
                     /locus_tag="OO322_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5908858.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVL"
     gene            complement(1184..1260)
                     /locus_tag="OO322_004250"
     tRNA            complement(1184..1260)
                     /locus_tag="OO322_004250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1224..1226),aa:Pro,seq:tgg)
BASE COUNT          331 a          347 c          309 g          314 t
ORIGIN      
        1 tttaacacgt tacaactgcg acactgcgcc ggtaattgct taaatttatc ttcaccaaac
       61 acctgctgtt gcggggaatc gatcatttct gcaattgttt gctgatgcat attccccagc
      121 cgatattgcg gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc
      181 accgagcgcc cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg
      241 gcaaacgccc attcgatatt catcacgaaa atcttgccga cgtcgcgttt gatccagtgg
      301 tcaaaaatcg ccaccagaaa ttcaccaaat tcatcggggc gcacagacca ttccgtcagt
      361 tccccctgaa tatcaccagg cgcatgtagt ttcagtccat cgcggacagc tgtttcatca
      421 gccaggcgct cgaccaccgg aataaactgg atgaattcga ctcccgcatc gcacaaaaaa
      481 tcataaacct gtaacggttg ctgcgcgctg gtgcggttga cgcagaccag cacgttatag
      541 tcgacatgat gtttttgcag gagcgtcagg gcacgcatca ccagtttatg cgtgggtctg
      601 ccgcctttgg tcacgcgata ttgattgtgg atctcagccg gaccatccag cgataaccca
      661 acaagaaaat tattttctgc cagaaatgca caccattcgt catcgagcag cacgccgtta
      721 gtctggaaac tgttacttat cttcctgcca gcaccatatt tcgcctgtag cgccacggca
      781 cggcggtaaa actccagccc gagtagcgtt ggttcgccgc cctgccaggt aaaagccact
      841 tcgttttgca gttcgctggc ggcgatatag tgacggatat acgcttccag cgtgtcatcg
      901 tccatatgcg tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag
      961 tcacagttca gattacaatc ggaaccgctc ggtttcgcta tcacatgaaa agcacgcgtt
     1021 ggaacctgtt gcagcatggt cgctccttgc ctgaagtcat ccttcaatac gttaagggcg
     1081 aaatcatacc gtggggcaat gagttattac gggcgtttat tgcgaaggga gggtgacgaa
     1141 atgcacagaa aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga
     1201 ttcgaacctc cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg
     1261 aaatactgct ttttgaattt ttagttcaat tctttaaagt c
//
LOCUS       DAJTRM010000258         1288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTRM010000258 DAJTRM010000000
VERSION     DAJTRM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.264"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            522..806
                     /locus_tag="OO322_004251"
     CDS             522..806
                     /locus_tag="OO322_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5908859.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="OO322_004252"
     CDS             806..1081
                     /locus_tag="OO322_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5908860.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          322 c          325 g          302 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag gacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc cagacatt
//
LOCUS       DAJTRM010000259         1279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRM010000259 DAJTRM010000000
VERSION     DAJTRM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.265"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..218
                     /locus_tag="OO322_004253"
     CDS             <1..218
                     /locus_tag="OO322_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908861.1"
                     /translation="GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSS
                     WTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            220..825
                     /locus_tag="OO322_004254"
     CDS             220..825
                     /locus_tag="OO322_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5908862.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            812..>1279
                     /locus_tag="OO322_004255"
     CDS             812..>1279
                     /locus_tag="OO322_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5908863.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGT"
BASE COUNT          300 a          301 c          359 g          319 t
ORIGIN      
        1 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
       61 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      121 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      181 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      241 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      301 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      361 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagtcg ctggctgacg
      421 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      481 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
      541 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
      601 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
      661 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
      721 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
      781 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
      841 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
      901 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
      961 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1021 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1081 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1141 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1201 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1261 ccttcagaat aaagggacg
//
LOCUS       DAJTRM010000260         1242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRM010000260 DAJTRM010000000
VERSION     DAJTRM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.266"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..1132
                     /locus_tag="OO322_004256"
     CDS             join(<1..750,750..1132)
                     /locus_tag="OO322_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5908864.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLSFRYLDHKTGETATETLTQRELVA
                     RLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLS
                     RDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          302 a          299 c          407 g          234 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaagtg tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg
     1021 cgtgctgtgt ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg
     1081 gctgaagaaa aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct
     1141 aagtgtgccc gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac
     1201 catgcagaga gagaaaacac cggagtggag ggaaaagcag aa
//
LOCUS       DAJTRM010000261         1214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRM010000261 DAJTRM010000000
VERSION     DAJTRM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.267"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..483)
                     /locus_tag="OO322_004257"
     CDS             complement(1..483)
                     /locus_tag="OO322_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5908865.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OO322_004258"
     CDS             complement(474..776)
                     /locus_tag="OO322_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5908866.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..>1214
                     /locus_tag="OO322_004259"
     CDS             1048..>1214
                     /locus_tag="OO322_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908867.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKM"
BASE COUNT          363 a          303 c          252 g          296 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctgggta caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaatacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgta
//
LOCUS       DAJTRM010000262         1188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRM010000262 DAJTRM010000000
VERSION     DAJTRM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.268"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            102..620
                     /locus_tag="OO322_004260"
                     /pseudo
     CDS             102..620
                     /locus_tag="OO322_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            987..1188
                     /locus_tag="OO322_004261"
                     /pseudo
     CDS             987..1188
                     /locus_tag="OO322_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          366 a          264 c          243 g          315 t
ORIGIN      
        1 ctctgaccac aatctgccgg ttacgccgaa tctgctgaat cagaacttta cgcccacagc
       61 acctaatcag gtctgggtcg cagattcagt tgtccaggcc ttccgaaatc aaccgactga
      121 aggcgctggt cgcgaaactg cagcgtatgc agttcggtaa aagctcagaa aaacttcgcg
      181 caaaaaccga acggtggata caggaagcac aggagcgaat cagcgcactt caggaggaaa
      241 tggcggaaac actgggtgag caatatgacc cggtactgcc atcttccctg cgccagtctt
      301 cagcccgtaa accgttacca gcctcacttc cccgtgcccc ccgggtcatc cggccggaag
      361 aggaatgctg tccggcctgt ggtggtgaac tcagtccact gggatgtgat gtgtcagagc
      421 agctggagct tatcagcagc gcctttaagg ttatcgaaaa acaacgccct aaactggcct
      481 gtcgccggtg cgaccatatc gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt
      541 atgccggagc ggggcttctg gcccatgttg ttaccggaaa gtatgcagac catctgccat
      601 tataccgcca aagtgatcta ctattccaca cagctattta gctctctttt tgttgagctc
      661 ccatgattct ttcatttatt gttactaatt attttatgtt atcatgatgt tagaacacgc
      721 tctgctctgt gtctctattt tatctatcat attctgagga ttaactacat aacaaggcgc
      781 aagatatata tacaaaaaca aataataccg ttgcaataat tccttttagt aaaggaggtg
      841 tgattaattt caggggattt cagagtaaaa attgtacaac atattaaaat atgttcattt
      901 cgttataata cacacaagga aatattaagt tagataatat attctaaata accatagata
      961 aaaacgcaca taatggggaa tattttatga aaatacctga agcagtaaat catattaatg
     1021 ttcagaataa tattgatctt gttgatggaa aaacgaatcc gaataaagca acaaaagcat
     1081 tacaaaaaat atattacgcg taacaaactc atcttcttct ggcataagtg aaaaacattt
     1141 agatcactgt gctaatactg tgaaaaactt tttaaggaag tctatagc
//
LOCUS       DAJTRM010000263         1158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRM010000263 DAJTRM010000000
VERSION     DAJTRM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.269"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>1158
                     /locus_tag="OO322_004262"
     CDS             <1..>1158
                     /locus_tag="OO322_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5908868.1"
                     /translation="NRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSF
                     VEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQ
                     DAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSL
                     NNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSP
                     AAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGAKV
                     ILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSA
                     ENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQN"
BASE COUNT          348 a          270 c          277 g          263 t
ORIGIN      
        1 aatcgcgtct ggccgacaaa agccgaaaac cacctgcgct ttaatatcat taatgataaa
       61 gaaaaccggg tgaacggcat cgcactctat tatcgcctgc caatggtaca agtgaacgat
      121 gagcaaagct ttgtcgaaca agctgaatgg agcatgttag ttcagctgtt caatcaacgt
      181 ctgcaggaac gcatacagtc gggcgagttg aagactattt ctggcggcac tgcgcgcagc
      241 gttaaaattg cacccgatta tcagtcgctg tttttccgtg taaatgcacg agacgataat
      301 atgcaggatg ctgcgaatgc attaatggca gagttggcaa ccattgatca gcatggtttt
      361 tctgctgaag aactcgatga tgtcaaatct acccgcctca cctggctgaa aaatgcggtt
      421 gatcagcaag ctgaacgtga tttacgtatg ctgaccagtc gcctggcatc cagctcatta
      481 aataatacgc cgttcttgtc gccggaagag acatatcaac tttcgaaacg tctgtggcag
      541 caaattaccg tgcaaagtct ggcggaaaaa tggcagcagt taagaaagaa ccaggacgca
      601 ttttgggagc aaatggtaaa caatgagctt gccgccaaaa aagcattgtc tcctgcggct
      661 atcctggcgc tggaaaaaga gtacgccaac aaaaagctgg cggcttacat cttcccaggc
      721 agaaatttat cgttaacagt agacgctgac ccacaggcgg aaattagcag caaagaaacg
      781 ctggctgaga atctgacatc attaacactt tccaatggtg ccaaggttat tctggcaaaa
      841 tccgcgggtg aagagcaaaa gctacaaatt attgccgtat cgaataaagg cgatttaagt
      901 ttccctgcgc agcaaaaatc acttatcgcg ctggcaaata aagcagttag cggaagcggc
      961 gttggcgaac tctcctcttc cagcctgaaa cgctggagtg cggaaaactc ggtaaccatg
     1021 agcagtaaag tcagtggcat gaatacgttg ctctccgtta gcgcgcggac taataaccct
     1081 gaacctggtt tccagttgat taaccagcga atcacccaca gcacgattaa cgacaatatt
     1141 tgggcatcgc tacaaaat
//
LOCUS       DAJTRM010000264         1152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRM010000264 DAJTRM010000000
VERSION     DAJTRM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.270"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(237..1145)
                     /locus_tag="OO322_004263"
     CDS             complement(237..1145)
                     /locus_tag="OO322_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908869.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKIEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          217 a          316 c          283 g          336 t
ORIGIN      
        1 ccctttcctt ctgtaaggcc ccttcctcgg gaggggcttt cccgtttcag ccctttgctg
       61 actccccgat tgttgatgag cgccggacct cgcgcaacgc tttcccagag gcagttttgc
      121 cttccccgag cggtcacgca aaacgttgca acgacctgat catttgcagt ttttagcgga
      181 acgcagatat aaaaaatccc cggctttttg tgccagggac gttcatagct gaaaatttat
      241 tttacgtttt cggcgacggg agttgttgag attaagggtg aagtcaatgc cgccagtgaa
      301 ccgcctgaaa cgccaatttc attgagcaag gagtcaatca gcggagcctg tgtgcggtaa
      361 gagagagcgg ctgataatgc ttgttccgct aggtttccgc cccccacatt acccgtgttt
      421 gcatcacccg cagcgctgcc actattcaga ccatcgacct gaataatctt gataccgtca
      481 atagatttca tcggctcaac ggatttttct attaccgcag gtagcgcctg caacaaggcc
      541 agtttgaatt taaggctggt ttgttcatca gaaagtacgt tgatagcatc gttcagcgca
      601 cgttgtgctt ctgcttccgc cagacccttt ttatgtgtag cttcggctaa ctcaacgata
      661 gccgccgcct gcatctctgc ggcttctttt tctgctttcg cccgcacggt cagttcaacc
      721 gctttggttt ctgcatcctg cgcggcagcg attagggcaa cttgtttagc acgatcggct
      781 tctgcggtct ggcgagtggt ttctacgttt tgttgcgcac ttactgcttc tgcgagtgca
      841 aggttagcac gcgcttctgc ctgggactgt tgttccgatt tggcggcaat agcgatcgat
      901 ttcgtctggt tggcgatttc ggtgacctgc tgctgttcga tctctttaat acgaacttca
      961 cgttcagcct caatctttct tgagcggacg gcctgctcgc gctcgatctc cgtttcctga
     1021 atttgtcgtt cagccagaat tcgcgtctgc tctgcttcac gacgacgttc agcttcaaaa
     1081 gccgcaatac gtgcattctg ttcggcagta cgggttttaa cctgctgctc ctgctcaagc
     1141 gtcataaacg ct
//
LOCUS       DAJTRM010000265         1149 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRM010000265 DAJTRM010000000
VERSION     DAJTRM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.271"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            114..>1149
                     /locus_tag="OO322_004264"
     CDS             114..>1149
                     /locus_tag="OO322_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5908870.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQK"
BASE COUNT          331 a          279 c          272 g          267 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaaga
//
LOCUS       DAJTRM010000266         1146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRM010000266 DAJTRM010000000
VERSION     DAJTRM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.272"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..550)
                     /locus_tag="OO322_004265"
     CDS             complement(<1..550)
                     /locus_tag="OO322_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5908871.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALIT"
     gene            complement(568..>1146)
                     /locus_tag="OO322_004266"
     CDS             complement(568..>1146)
                     /locus_tag="OO322_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5908872.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
BASE COUNT          302 a          279 c          235 g          330 t
ORIGIN      
        1 ctgtaattaa tgctgcgaca ctcttacgtg atacgatcgt acctttgaat ggttcattgc
       61 gagaggtaag ctcataatca ataatatctt catcagtcag ccaggcaggg cgaagaattg
      121 tatattccag tccagaagcc tcaattgcat ccgcagccct gcggaatggt tttaggggtt
      181 caccaataac tgcattattc cattcgacaa attttcctgg aacttcatca taaataccga
      241 gggaaagaac aaagatcaga cgtttaacat cacaggcttt catcgcagcg atcactgaat
      301 ttgcctgaat atctaaatct tcccccgtaa ggttggcata cacaatatcc tgtccctgca
      361 tggcctgctt cagtgctgca tgattcagta catcccccat aataatctgg ctattagttg
      421 gatagggttt gtgtattttt gctggctgac gggcaaataa agtttgttta atagtttgtt
      481 tatctgccag ttgattaatc acatggcggg ctatctgacc gccagcacca agaatcagta
      541 catttttcat aatatttctc atgaaactta tcccagatgg gtgttgaaaa aaggaataac
      601 ttttgccagc gcctcctgaa ctgcttccgg tttgtcatat agttcataat gtgagaaatc
      661 gggtaacgaa actaactgac gatcctgcga aaccatcgcc cggccataga cttccagtcc
      721 atcacgataa gcaccgaagg caccgacttt ttcaccaact actaccatca ccggctgggt
      781 cagtaacact tcagtaaatg cgaaggcatc ccaggccagc gttttctgtg catgtgagaa
      841 caacattttt gtcgcacctc ccggttgttg cccgcgtggt gttttatagt agtcagttgc
      901 ttcataaaca tcgcgttcgg tcagtccatg ggcttttgct gcatccaggc tggcgggaag
      961 taattcgttg atctgtaatt ctccaccacg agcctctttt gctcgttgtg acgccattgc
     1021 attcaatacg ccaatcggat cataattgct gaaaccttcc cgaaaaagac gcccgatatt
     1081 aacacccgta atactcacta cagctttaat tcgtttttct gtcagcgcgg cattaatggc
     1141 atagcc
//
LOCUS       DAJTRM010000267         1111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRM010000267 DAJTRM010000000
VERSION     DAJTRM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.273"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..323)
                     /locus_tag="OO322_004267"
                     /pseudo
     CDS             complement(1..323)
                     /locus_tag="OO322_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            246..497
                     /locus_tag="OO322_004268"
     CDS             246..497
                     /locus_tag="OO322_004268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908873.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(517..>1111)
                     /locus_tag="OO322_004269"
     CDS             complement(517..>1111)
                     /locus_tag="OO322_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5908874.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          259 a          274 c          275 g          303 t
ORIGIN      
        1 atcaagttca cagataaggg caacatcagc gtgagcgact ggcgcagatg ttatccgccg
       61 gggcgagagc tttttaatgt ccgtatgacg gtattgatgc cgattttcag tgtcctggcg
      121 gtatcacggc cccctgcgcc gttatgtgcc atttcaacga tctgctcctt aacgccgagt
      181 tttctggctt cataagagta agtgagctgg aaaacacgtt tacatgagcg acaacggaaa
      241 cgctcatgcc gggaacaact acggccatgt cgataaactt catcggaatt acagcgcgga
      301 caatgaacag taacggtagt catggaaaac ctcaaatacc agtagattac cagaaacttc
      361 tatcgattga agacattacc ttttgccgca ataggtgggg taacattggt gaaaacgcat
      421 tacgccgggt ggcagttgga aaaaaattat cttttttcgg ctctgatcgt ggtggagaaa
      481 atgcggcaat aatctaaata gctgcgctta ataccgctac acttttgcca gcccatgttt
      541 gcctccgggg aatgggctga cggtttccat aaaatggcga acttttttca acagttgcca
      601 cattgagcgg cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc atagccgttc
      661 aacatgattc acccatggcg agtaaaccgg ctgataaatg cccctgaact tcgggttctc
      721 cttcagccag ctctgtgttt cccggctttt gtggataatg tagttgtcca cgatcagcgt
      781 gatggttttc gcacgacggt atgtcgcttt aagccgcttc agcaggctga tgaacagcgc
      841 cgaacttttg ctgttgccgc ccacatagct gactttacct gtcccgctgt gcagcgctcc
      901 ggccagataa tatttttcat tctgtcccgg cgtgaccacc cgtttttgct gtccgcgcag
      961 ttgccagtcc gcaccgattt tgggatgaag atggatatcc acttcatctt cataaaagac
     1021 cggatgctct gcgctgcatt cgtccagtgc tttatggatt gctgccatct tttcatcttt
     1081 atgcgggtca cggatacgca gagttggcgc a
//
LOCUS       DAJTRM010000268         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRM010000268 DAJTRM010000000
VERSION     DAJTRM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.274"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            55..>1100
                     /locus_tag="OO322_004270"
     CDS             55..>1100
                     /locus_tag="OO322_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5908875.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTRM010000269         1075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRM010000269 DAJTRM010000000
VERSION     DAJTRM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.275"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>1075
                     /locus_tag="OO322_004271"
     CDS             <1..>1075
                     /locus_tag="OO322_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5908876.1"
                     /translation="PIDVLFRPKDGANPDAADVLMGMYRTDMRHNTAKIAVNVGVREQ
                     IESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDANSKQMDKSDAKHCTVI
                     NALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAEYYEVEEKKEKVY
                     IYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKSIITCTHILKDRE
                     KIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIMSFNADIVARSPKKKP
                     TFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEA
                     ATNAVKEVASLGVDAQAANGQVA"
BASE COUNT          296 a          259 c          284 g          236 t
ORIGIN      
        1 accctatcga cgttctcttc cgacccaaag acggcgctaa tcctgatgct gccgatgtgt
       61 tgatgggaat gtatcgtact gatatgcgcc ataacacggc aaagattgcc gttaacgttg
      121 gcgttcgtga gcagatagag tccggcgttg gtgcatggcg tctggtcacc cagtacgaag
      181 acaacgatcc aacaagcaac aatcaggtaa tccgacgcct gccaatccat gaagcctgct
      241 cacacgtcat atgggacgcc aacagcaagc agatggataa gagcgacgct aagcactgca
      301 cggtgattaa cgctttgtca cgcaatggct ggaaagagtt cgcagaggat tacggtattg
      361 atcctgacac cttgccatct ttccagaatc cgaacgatac atggctgttt ccgtgggtgt
      421 cgaatgatgt cgtctacgtc gctgagtatt acgaggtcga agagaagaag gagaaagtat
      481 acatctaccg cgacccgctg acaggtgagc cggtcagcta ttaccaacag gatatcaaag
      541 acgtcatcga cgacctggct aatcgtggat tcattaaggt agcagagcgc aaggtgaagc
      601 gtcggcgtgt gtataagtcg atcatcacct gcacgcatat actgaaagac cgcgagaaga
      661 tagctggaga gcatatccca atcgttcctg tgtatggcga atggtcattt gctggtgaca
      721 aggagtgcta cgaaggtgta gtaaggctga cgaaagacgg tcaacgcctt cgtaacatga
      781 tcatgtcatt caacgccgat attgttgctc gttcaccgaa gaagaaacct accttcttcc
      841 ctgagcaaat cgaaggctac gaatacatgt acggtggaaa tgatgactat ccgtactatc
      901 tccagaacag gactgatgaa aacggtaacg acctgccgat tggtccaatc tcctacatgg
      961 aaaaccctga agtgccgcaa gccaacgctt acatgcttga ggcagccacc aacgcagtga
     1021 aagaggtggc tagtcttggc gttgatgcgc aggcggcaaa tggtcaggtc gcttt
//
LOCUS       DAJTRM010000270         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRM010000270 DAJTRM010000000
VERSION     DAJTRM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.276"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(11..814)
                     /gene="dkgB"
                     /locus_tag="OO322_004272"
     CDS             complement(11..814)
                     /gene="dkgB"
                     /locus_tag="OO322_004272"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5908877.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(977..1053)
                     /locus_tag="OO322_004273"
     tRNA            complement(977..1053)
                     /locus_tag="OO322_004273"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1017..1019),aa:Asp,seq:gtc)
BASE COUNT          279 a          250 c          241 g          298 t
ORIGIN      
        1 tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc
       61 aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa
      121 gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca
      181 tagcccacgc cagaatcact tgtgccggaa tcgcattgtg tttagctgca atacgagcaa
      241 taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg atatgaatac
      301 cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag agttcaatct
      361 ggttggtagc gatgttttca gcgccaacag cagcaatcac tttttccatc aatgggatcg
      421 tgaagttgga aataccgatc tcacgcgtca gcccttgttt tttggcttcc agcagcgcct
      481 gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat
      541 caacataatc ggtacgcaat ttttgcaggc tctctttcag actcgggatc aatttgtctt
      601 tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac
      661 tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgcg gtatcaattg
      721 cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg tctttcagac
      781 ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa tgctattttc
      841 gttaactgag gtgattatgg gcttcagact gttgcgggaa aagagcgtaa aacgcagagg
      901 atttttgcga ttctagcaat aatagatatc cagacgcaaa aaaacatcga gcgttaactc
      961 gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
     1021 ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttac
//
LOCUS       DAJTRM010000271         1059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRM010000271 DAJTRM010000000
VERSION     DAJTRM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.277"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            35..310
                     /locus_tag="OO322_004274"
     CDS             35..310
                     /locus_tag="OO322_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908878.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            410..>1059
                     /locus_tag="OO322_004275"
     CDS             410..>1059
                     /locus_tag="OO322_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5908879.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMT"
BASE COUNT          338 a          237 c          238 g          246 t
ORIGIN      
        1 agggattcgc tcaaccttaa ttaaggagaa aaacatgatt accagttatg aagccactgt
       61 tgtaactact gatgacattg ttcacgaggt taatctggaa ggaaagcgta ttggctacgt
      121 gattaagaca gaaaataaag aaaccccatt cactgtggtt gatatcgacg gtccatcagg
      181 caacgttaaa acacttaacg atggtgtaaa aaaaatgtgt ctggtgcaca taggaaagaa
      241 tctgcccgca gaaaaaaaag ccgaatttct ggcaactctg attgcaatga aattaaaagg
      301 tgaaatctga aaaaaagaaa gcctgcacac tgtgcaggcc tgaatgaaga acctgggaca
      361 tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa tgaacaccga
      421 taaacaagtt tacccactgt attacgaagc aaaaaatgac aaagtaagaa aacgtctcgg
      481 tattaaaggc ggtttttact gggctgaagc gaaaaaatta tccattgcca tctcccgtgg
      541 tgctgttgcg attgacgatg caggctacga tgaagatgac tttaaaaaac ctgttcgcgt
      601 caatttgccc gttgttgatg acctcccgcc agaaggcgta tttgatacgg aattctgcaa
      661 ccgttacgaa aaaggcgggg aagatggcat cacaatggta tttatcgcat cttctccctc
      721 tgttcaggac aaaccggcca gcactgacaa taccaacgtc aacggcgaag acatgacaga
      781 aattgaggag aacatgctcc tgccagtttc cggtcaggaa ctgcccattc gctggcttgc
      841 gcaacacggc agcgaaaaac cagtaacgca cgtttcacgc gacgaactcc aggcattaca
      901 cattgctcgg gatgaagaac taccagctgt tactgccctg gctgtttccc acaaaaccag
      961 cctgctcgat ccgctggaaa ttcgcgatct ccacaaactg gtgcgtgata ctgacagagt
     1021 tttccctaat ccaggcaatt caagcctggg gctgatgac
//
LOCUS       DAJTRM010000272         1017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRM010000272 DAJTRM010000000
VERSION     DAJTRM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.278"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO322_004276"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO322_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5908880.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          327 a          148 c          176 g          366 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcg
//
LOCUS       DAJTRM010000273          992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRM010000273 DAJTRM010000000
VERSION     DAJTRM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.279"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>992
                     /locus_tag="OO322_004277"
     CDS             <1..>992
                     /locus_tag="OO322_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5908881.1"
                     /translation="CGAALMFWLDMFDVQIMAQTLVNGADSFSLLAIPFFVLAGEIMN
                     AGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAMIMASLSGSAVADTAAVAALLVPMMRS
                     ANYPVNRAAGLIASGGIIAPIIPPSIPFIIFGVSSGLSISKLFMAGIAPGIMMGATLM
                     LTWWRQASRLNLPRQQKATMQEIWHSFVSGIWALFLPVIIIGGFRSGLFTPTEAGAVA
                     AFYALFVATVIYREMTFATLWHVLIGAAKTTSVVMFLVASAQVSAWLITIAELPMMVS
                     DLLQPLVDSPRLLFIVIMVAILIVGMVMDLTPTVLILTPVLMPLVKEAGIDPIY"
BASE COUNT          176 a          258 c          272 g          286 t
ORIGIN      
        1 tgcggggcgg cgttaatgtt ctggctggac atgtttgatg tccagatcat ggcacaaacg
       61 ctggtgaacg gagccgatag cttctccctc ctggcgattc cgttctttgt gctggcgggt
      121 gaaatcatga atgcgggcgg cttgttaaaa cgcattgttg acctgccgat gaaactggtg
      181 gggcataaac caggcgggct gggctacgtc ggcgtgctgg cagctatgat tatggcgagc
      241 ctttctggtt ctgccgttgc ggataccgcc gccgttgccg ctctactggt gccgatgatg
      301 cgcagcgcca actacccggt caaccgggcg gcggggctga ttgcctctgg cggcattatc
      361 gcgccaatta ttcccccttc cattccgttt attatcttcg gtgtttccag cggattatcc
      421 atcagtaagc tgtttatggc gggcattgcc cctggcataa tgatgggcgc aacgctgatg
      481 cttacctggt ggcggcaggc cagtcgcctt aatttacccc gccagcaaaa agcaacgatg
      541 caggaaatct ggcactcttt tgtctccggt atctgggcgc tgtttcttcc agtcatcatt
      601 attggcgggt ttcgctccgg tctgtttacc cctaccgaag ccggggccgt tgctgctttc
      661 tatgcgctgt ttgtcgccac agttatttac cgtgaaatga ccttcgccac actttggcat
      721 gtactcattg gcgcggcgaa aaccacctca gtggttatgt ttctggtggc ctcggcacaa
      781 gtttccgcct ggctgattac cattgctgaa ctgccgatga tggtttctga tttactgcaa
      841 ccgttggtcg attcaccgcg tctgctgttt atcgtcatta tggtggctat cctgattgtc
      901 ggcatggtca tggatttaac gccgacggta ttaattctta ccccggtatt aatgccttta
      961 gttaaagagg caggaatcga tccgatttat tt
//
LOCUS       DAJTRM010000274          985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTRM010000274 DAJTRM010000000
VERSION     DAJTRM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.282"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            429..>985
                     /locus_tag="OO322_004278"
     CDS             429..>985
                     /locus_tag="OO322_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5908882.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          354 a          188 c          159 g          284 t
ORIGIN      
        1 attgatgaaa aacaaagcca acggtattct gatttctgaa gttaatttca gactcgttta
       61 agctccattt tcaaaaaaaa tatatcaggg tttgttacta ataacttctg aaaacagaaa
      121 aaaatagagc agtaattcta tttcagagag gagtaactcc atctatcctc gatacgtact
      181 gaacgttatc gtgatatatt catcatgctt tcttcaccat tattcgagta tagggagaga
      241 cgcaatgacc ttaaatacct ttttatggct gaattaccat tctcaggaat aacagcagac
      301 tccttcccat agacggctta gggggaaagc aatacccgga gaaagggtac aaagcaaact
      361 gttttaaaat taataagcca tattctattt aactagaatt cttatcgttg ataagaattc
      421 tagttataat gattaaatca accaatatac aggcaatcgg ttctggcatt atgcatcaaa
      481 taaacaatgt atactcgtta actccattat ctttacctat ggaactgact ccatcttgta
      541 atgaatttta tttaaaaacc tggagcgaat gggaaaagaa cggtacccca ggcgagcaac
      601 gcaatatcgc cttcaatagg ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt
      661 tatctgagtt agatttaaaa acattaccag atttaccgcc tcagataaca acactggaaa
      721 taagaaaaaa cctattaaca catctccctg atttaccacc aatgcttaag gtaatacatg
      781 ctcaatttaa tcaactggaa agcttacctg ccttacccga gacgttagaa gagcttaatg
      841 cgggtgataa caagataaaa gaattaccat ttcttcctga aaatctaact catttacggg
      901 ttcataataa ccgattgcat attctgccac tattgccacc ggaactaaaa ttactggtag
      961 tttctggaaa cagattagac agcat
//
LOCUS       DAJTRM010000275          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRM010000275 DAJTRM010000000
VERSION     DAJTRM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.283"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..566
                     /gene="tetR(B)"
                     /locus_tag="OO322_004279"
     CDS             <1..566
                     /gene="tetR(B)"
                     /locus_tag="OO322_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5908883.1"
                     /translation="GIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHH
                     THFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQ
                     QGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQ
                     GAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(544..>932)
                     /locus_tag="OO322_004280"
     CDS             complement(544..>932)
                     /locus_tag="OO322_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5908884.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
BASE COUNT          297 a          189 c          193 g          253 t
ORIGIN      
        1 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
       61 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      121 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      181 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      241 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      301 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      361 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      421 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      481 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      541 aacttaaatg tgaaagtggg tcttaaaagc agcataacct ttttccgtga tggtaacttc
      601 acggtaacca agatgtcgag ttaaccaccc tttagattca taaagcgaaa ataatgcggc
      661 tccaacgtac ccacctaaat ggaaacggcg ttcactccaa tctaaacacg cacaacagat
      721 tttacgtgaa tgtttggaag gaacgtcaat tcccatttca tgaaaatatt gaataccact
      781 taatgtgatc attgaaccat tttcagtgat ccattgctgt tgacaaaggg aatcatagat
      841 cttaacggca acttcgccag ctaaatgatc atagcaagta cgtgcttttc gtaaatgcac
      901 tggcgtggaa actttggcat gtacgccatg gt
//
LOCUS       DAJTRM010000276          924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRM010000276 DAJTRM010000000
VERSION     DAJTRM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.284"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..218
                     /locus_tag="OO322_004281"
     CDS             <1..218
                     /locus_tag="OO322_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5908885.1"
                     /translation="LRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWL
                     REVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            407..592
                     /locus_tag="OO322_004282"
                     /pseudo
     CDS             407..592
                     /locus_tag="OO322_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(692..>924)
                     /locus_tag="OO322_004283"
     CDS             complement(692..>924)
                     /locus_tag="OO322_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5908886.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          259 a          226 c          228 g          211 t
ORIGIN      
        1 cgttacgatc ggtggcggtt ggaagaaaaa attatctctt tttcggctca gacaagggag
       61 gagaaagtgc ggcgatcatc tacagtctgc tggtcacctg caaacagaac gaagtggagc
      121 cggaggactg gttgcgcgaa gtgatcgaga agctcaatga ctggccgtcg aaccaagtgc
      181 atgaactgct gccctggaac ttctcgtctg taaaataatc cttacgctac gtacttctcg
      241 gggcgcttac catacggtgg tcggtttacg cttaccgcag gacaggtggt aactgaaaaa
      301 tagtttgctg aggcgactgg aaacagaagg gcaagatcga ttccgacagc agtgatggtc
      361 agctacctca tcgataactt tgcaacagtg cccttcttga agcatcatgc ttcatgaaga
      421 aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag ccttcccggc
      481 ggtgaaaacg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc agaagcagct
      541 ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagttt tacaggggac
      601 atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc atggaggaag
      661 gggatatatc gcagctctga cgactcactc cttatttacc cgggaaatac ttctccagca
      721 atgcatacag gatggcagcg gtttcagcat ccattatgcc gtcataattt ttctgccgga
      781 aatgaagctg aaaagcgcgg acaagcgcct tatatccgtc atccgtctcc acaccagaaa
      841 tactgtatcc ataggtcttc agccgtgaca cgatatcaac cttatccgga atgccctgcg
      901 attcaaaaaa taaatattta gtca
//
LOCUS       DAJTRM010000277          915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRM010000277 DAJTRM010000000
VERSION     DAJTRM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.286"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..546
                     /locus_tag="OO322_004284"
     CDS             1..546
                     /locus_tag="OO322_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5908887.1"
                     /translation="MLHLKNITTGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            570..>915
                     /locus_tag="OO322_004285"
     CDS             570..>915
                     /locus_tag="OO322_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5908888.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQ"
BASE COUNT          261 a          196 c          247 g          211 t
ORIGIN      
        1 atgttgcatt taaaaaatat tactacgggt aatccgaaaa cagcagaaca atatcagatg
       61 acaaaacgat attcggtcac ctggcttttt tcagaagacg gaaaaaactg gtatgaagag
      121 ctgaagaatt tcgccagcga cacaataaaa atagcttaca ccggagatgg tcgcgtggtg
      181 tgggtcggta aggatgtgac aggcatcgag ccacgcaatg ccagcgttat cgaagttccg
      241 gatattaccg ctaaccgacg gattaccgcg ccgggttact ggttttaccg caatgatgaa
      301 tttgtctttg actacagact caaagcggaa gatgagcgtg atgcccttct ggctcaggtc
      361 agtgcccgga caggggaatg ggaagaagac ctgctgctgg ggttaatcag cgacgaagat
      421 aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc tgcaggcgat ggatttcagc
      481 accatcactg ataaatcctc atacaacgcc attgaatggc ccgcctctcc ggaaggttct
      541 tcctgattta atttatcgcg agaaaaacaa tgtctgtagt gatatcaggt gcgctgactg
      601 atggcgcagg tatccccatg tccggatacc atattattct gaaatcccgg gtaaacaccc
      661 cggaagtggt gatgaacact gttgctgatg tgatggcagg aaacgatggt gaatactgtt
      721 tccatgcgcg gactggaaaa tatggtgtgt atctgaaaca ggactggcgc aacgagtaca
      781 acgttggcga cattgctgta tatgaggact caaagcccgg cacgctgaat gactttctga
      841 ttgctcctga tgagggcgac ctgaaaccgg atgtcgtcaa acgctttgag gaaatggtgg
      901 cgcaggcgca gcaga
//
LOCUS       DAJTRM010000278          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRM010000278 DAJTRM010000000
VERSION     DAJTRM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.288"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..257)
                     /locus_tag="OO322_004286"
     CDS             complement(<1..257)
                     /locus_tag="OO322_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908889.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="OO322_004287"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="OO322_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5908890.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="OO322_004288"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="OO322_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5908891.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          240 a          175 c          220 g          229 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttga
//
LOCUS       DAJTRM010000279          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRM010000279 DAJTRM010000000
VERSION     DAJTRM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.289"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            163..>860
                     /locus_tag="OO322_004289"
     CDS             163..>860
                     /locus_tag="OO322_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5908892.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRR"
BASE COUNT          215 a          203 c          224 g          218 t
ORIGIN      
        1 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
       61 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
      121 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
      181 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
      241 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
      301 aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga tgtttgtcgt
      361 ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc actcacagca
      421 aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg taatacgacc
      481 tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac ttattacgac
      541 ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga actggatggc
      601 gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga ctatttcaag
      661 gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc agcgaaggca
      721 ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta tgccttgcgc
      781 atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc taaagaaccg
      841 cttgggccgg tagttcgtcg
//
LOCUS       DAJTRM010000280          857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRM010000280 DAJTRM010000000
VERSION     DAJTRM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.290"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..236)
                     /locus_tag="OO322_004290"
     CDS             complement(<1..236)
                     /locus_tag="OO322_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5908893.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQA"
     gene            complement(246..>857)
                     /locus_tag="OO322_004291"
     CDS             complement(246..>857)
                     /locus_tag="OO322_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5908894.1"
                     /translation="SDKSAKYAAEAQKYAADLQNQQWQTIMKNLAPFTPLAEQYVNQL
                     QNLSSLEGQGQALNQYYNSKQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPT
                     LGQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSS
                     LQTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          193 a          240 c          203 g          221 t
ORIGIN      
        1 gcctgctgaa attctttctg ccgctgagcc tgcttttcct gctgaaaaac acccgcaata
       61 cctgaaagac cttgcaaagc agtcagccca acattgttag cgcctgaacg ctcaatatca
      121 ttgttctgcc tgataagctg aagcgtattg ccgatgtcat ttacgctcgg agcgtttgag
      181 ttgacgccgc cgataccagc caacaatccg ccgtttgttc cttgccaagt agccatgatt
      241 cccccttaaa acaacgagcc aagcaatccg ataccagcac caataccagc gccccaaggc
      301 gttgatgttc ccaaaaggct ggcaagacct gcaccggcaa tcgcaccaga cgtgccaccg
      361 ctaattgcag tctgaagact tgatggttta ttggcattag cagcggcaag agctgcgctt
      421 tgctgtgcaa tgctgctcat gttgttggcg tacgtctgcc cggcgtttgc ctgaccttgc
      481 agcgcaccaa gcccaacgtt tgccagattg ttgtaattgc tcatctgatt tgataaccaa
      541 gactgaccga gtgtcggcgc gatcgtagcc agttgattgc ttgtggctgt cgaaccaagt
      601 ccacccgtcg cctccgcagc agcaagactc tggtaacgcg cctgccctgc aaggtcttta
      661 tactgcttag agttgtaata ctgattaagt gcctgcccct gaccttctaa actggaaagg
      721 ttctgaagct ggttaacata ctgctccgca agaggcgtga acggagcaag gtttttcatg
      781 atcgtctgcc actgctgatt ttgcaggtct gcagcatact tctgggcttc tgctgcatac
      841 tttgcgcttt tatcaga
//
LOCUS       DAJTRM010000281          849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRM010000281 DAJTRM010000000
VERSION     DAJTRM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.291"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..585)
                     /locus_tag="OO322_004292"
     CDS             complement(<1..585)
                     /locus_tag="OO322_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5908895.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVR"
     gene            complement(582..>849)
                     /locus_tag="OO322_004293"
     CDS             complement(582..>849)
                     /locus_tag="OO322_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5908896.1"
                     /translation="SQALANFTGFVVAVSFSFFANARFTFKALTTAMRYMLYVGFMGI
                     LSVIVGWAADKCSLPPIVTLITFSAISLVCGFVYSKFIVFRDAK"
BASE COUNT          244 a          203 c          172 g          230 t
ORIGIN      
        1 gcgtacatat tcaacgacat ccgtcggacc acccacccag ctcagtatgc ctttcatgaa
       61 aagattgcgc tcaggcaaca gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa
      121 atctccgaca ttttcctcga tctttggggt gcttattttg ttgtgtaatt tatagaacca
      181 ctcagctgtc ttacgtttca gtcgtccatc agttgagcgg tctgaacgtt tagcaagcac
      241 catgtcagca cctgcctgcc acttttcaat aagatgaggg ataacctcaa ttgggtcttg
      301 caggtcgaca tcaataggaa ttacagcatc gccgcttgca tggtctaacc ctgcaaataa
      361 ggctggttct ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac
      421 agccagcgcg ttaataattg actctgtggc atctttactt ccgtcattta tgaatacaat
      481 ttctacttca tatggcttca actcttggaa ttcacgtacc gttttataga aaacaggtat
      541 cgcttcttct tcattgaaga caggaacgac aagagatatt ttcatttcgc atccctaaag
      601 acaatgaact ttgaatagac gaaaccgcac accagactga tggcggagaa ggtgataaga
      661 gtgactattg gaggaagtga gcatttatca gctgcccaac caacaatcac actcaatatt
      721 cccataaatc ccacgtataa catgtagcgc atcgctgtag tcaatgcttt gaatgtgaat
      781 cttgcattcg cgaagaagct aaagctcaca gccactacga aacctgtgaa gtttgccaga
      841 gcctgactg
//
LOCUS       DAJTRM010000282          833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRM010000282 DAJTRM010000000
VERSION     DAJTRM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.292"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            43..833
                     /locus_tag="OO322_004294"
                     /pseudo
     CDS             43..833
                     /locus_tag="OO322_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          215 a          210 c          221 g          187 t
ORIGIN      
        1 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
       61 cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
      121 agcgttgcaa cacgtctcga tatcaccacc cacagccttt atgcctggat aaagaagtac
      181 ggtccggatt cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc
      241 cagaaagagc tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac
      301 ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg
      361 ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc
      421 agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct
      481 ggctggaatc gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgtgacagcg
      541 ggcaacagtg cggagtaaac agagtctgga gactgatgaa acgtgtcgga ataaaggctc
      601 aggtcggata ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca
      661 ggctccagcg acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct
      721 acatcaggac ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca
      781 aaattatcgg ctggtccatg caatcccgga tgacaaagga cattgtcctg aac
//
LOCUS       DAJTRM010000283          795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRM010000283 DAJTRM010000000
VERSION     DAJTRM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.293"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            178..651
                     /locus_tag="OO322_004295"
     CDS             178..651
                     /locus_tag="OO322_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5908897.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          249 a          162 c          173 g          211 t
ORIGIN      
        1 ccggcaaaag tgcctgacct gacccctatt agcggttacc agcgccccac cagacccggt
       61 gacatcaccc cccggatcat ggacgactgg gggcagtaat catgccgggt ctggctggct
      121 aacaccccat tataaggggt acgaataaag taacgggggg gagatgaacc ctatgcaatg
      181 tcctggttaa tatctcagtg tgctcatcag tgcactgata ataaaaagac ggagacagat
      241 gccatttacg ataaagttcg gagttcttat ttgctttcat gtattctgaa aaaaaacaaa
      301 aatgttgggc ttattcttca tgcaccatca tttgtttctg tgtctgaaaa aatcgccaga
      361 attgtgatgg caaattactc ccgtaactgg agtaattccg agttggccag tgcggtttta
      421 atgagtgaat cttctttaaa aagaagaatg tacaaagagg tcggttctat aagtacattt
      481 gtacataaaa taaagcttac agaagctatc aggaaattac gaaggacaaa tactcccatt
      541 tctgtcatat catctgagtt aggttacagt tcaccatcgt atttttctaa agtcttcttt
      601 aaatatttaa agacataccc tcaaaacata aggaagaaaa atggtcggta atgtcaccaa
      661 ttaggaggac ttgcagagcc gcttttttac gatactgaga tcaaacagac ataatctatg
      721 cggagattta cagcagggca tggttcacaa atctgacagc gatgaattat cggcactgcg
      781 ggcagaaaat gctcg
//
LOCUS       DAJTRM010000284          786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRM010000284 DAJTRM010000000
VERSION     DAJTRM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.294"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..786
                     /locus_tag="OO322_004296"
                     /pseudo
     CDS             1..786
                     /locus_tag="OO322_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          207 a          203 c          181 g          195 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgcca
//
LOCUS       DAJTRM010000285          762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRM010000285 DAJTRM010000000
VERSION     DAJTRM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.295"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>762)
                     /locus_tag="OO322_004297"
     CDS             complement(<1..>762)
                     /locus_tag="OO322_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5908898.1"
                     /translation="VVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG
                     FLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYE
                     FICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMR
                     IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          176 a          215 c          217 g          154 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg cccctgtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacccaggt agtcaacgtt gaacaccagt
      481 tcctgcggca gcgcttcttc gtcacgccag ataacccagc cgcagcccag cggagccaga
      541 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg
      601 atatccgggg cgacgaacgg tgccaggaag ccaccgctgg cagcgtcgat gtgcatgtcg
      661 atgtcgatac cggtgtcggc ctggaattta tccagcgcat cgtgcagcgg ttgtgggaac
      721 tcatagttac cggtgtaggt cacgccgaaa gtcggcacca cg
//
LOCUS       DAJTRM010000286          756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRM010000286 DAJTRM010000000
VERSION     DAJTRM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.296"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..379
                     /gene="ospI"
                     /locus_tag="OO322_004298"
     CDS             <1..379
                     /gene="ospI"
                     /locus_tag="OO322_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5908899.1"
                     /translation="SEYMTGFFHRHLEQIDSEGIVSHPNETYSKFRERIAENILQNTS
                     KGSVVMISIEQATHWIAGFNDGEKIMFLDVQTGKGFNLYDPVEKSPDAFVDENSSVQV
                     IHVSDQEFDHYANSSSWKSKRLC"
     gene            494..756
                     /locus_tag="OO322_004299"
                     /pseudo
     CDS             494..756
                     /locus_tag="OO322_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          235 a          123 c          178 g          220 t
ORIGIN      
        1 atcagagtat atgaccggat tttttcatag gcatttagag caaattgata gtgaaggcat
       61 agtgtcacat cctaatgaaa cttatagtaa atttcgtgag cgtattgcag aaaatatttt
      121 acaaaatact tcaaaaggaa gtgtagtaat gatttctatt gaacaggcca ctcattggat
      181 agctggattt aacgatggtg aaaaaattat gtttttggat gttcaaacag gaaagggatt
      241 taatctttat gatcctgtag aaaaatcgcc agatgcattt gtagatgaaa atagttcggt
      301 tcaggtaatt catgtatctg atcaggaatt tgatcactat gctaacagta gtagttggaa
      361 aagtaagagg ctttgctgat gctgagagcc tgtacataga tttgagccgg ccccttgaaa
      421 cgccagacag tggatgtatc ttaaaataag agataggctt ggatatatgt ctaacactag
      481 ttctaatttt gagatgactg gaactttgct ggggctggag cttcgtaaac gtaaaactcc
      541 tcaggaaaaa attgccatta ttcagcagac aatggagcct ggcatgacgg tgtcccatgt
      601 ggcgcgtttg catggtattc agcccagcct gttgcttaag tggaaaaaat aatatctgga
      661 aggaagtctc actgctgttg ctgccggaga ggatgtcgtt cctgcctcag aactggctgc
      721 cgctataaaa caaataaaag agttacagcg cttgct
//
LOCUS       DAJTRM010000287          741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRM010000287 DAJTRM010000000
VERSION     DAJTRM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.297"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            68..>741
                     /locus_tag="OO322_004300"
     CDS             68..>741
                     /locus_tag="OO322_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5908900.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTS"
BASE COUNT          195 a          179 c          183 g          184 t
ORIGIN      
        1 tgtccatgtg atattgtgta ccagatgtgt gccaaactat tatttctgag cgtaccaaat
       61 attatttatg gctataagcg acacaaaact gcgtactatt tatggtaaac catattcggg
      121 cccacaagaa gtggctgatg ccgatggcct cagcgtacga atttcaccga agggggtcat
      181 ccagttccag taccgctatc gctggcatgg caagcctaat cgacttgggc ttggtcgata
      241 cccatccctg tctttgaagg atgccagaca gatcactgct gacttgcgaa agctctattt
      301 ctcaggaacg gatccacgca cctattttga agagaaggtg gagaactcca tgacggtcgc
      361 ccagtgtctc gactactggt tcgacaacta cgtctctaca actctcagag aaaagaccca
      421 ggcactttat cgatcaacgg ttatgaagcg catgcatgac gccttcccta atcgtccggc
      481 atcttctatc acggttaagc aatgggttga cctgcttacc gaagaagaaa aagataatcc
      541 acgccgagca aggcaggtgc taagtcaact aagatcagca attagttggt gcatgcggcg
      601 tcagttgata gatagttgcg caattatgag catccagcca agggacttcg gctcccgcgc
      661 tgaggtaggg gatcgggtac tgtcgtatca cgaactggct aagatttggc ttgctattga
      721 aagaagccgt gcgtctacgt c
//
LOCUS       DAJTRM010000288          705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRM010000288 DAJTRM010000000
VERSION     DAJTRM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.298"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          221 a          147 c          144 g          193 t
ORIGIN      
        1 gtcacttaag cgtatggtca acctgacaac ccggtgtcct caacggggaa ggaataactc
       61 cgccatactt accgccgcgt catttcgcgt tgtgtgcctg cttttaacta cgtcaggcga
      121 ggtggttcct gttattcccc aacaacaaga aatttgtata atctgaatac cccaacaata
      181 agaagagtga gcaagtgatg tattagcgat gtcagctctc aaagagactg catcaatgtt
      241 aaaattattc aatgaagcaa aaactcaggc tgaggttgat cgcgctgtgt ctgaaataaa
      301 ctctaagttg tcaagcattc agcttgaata tttgtcatct cattgatgag ctgacaatat
      361 ctaaaagcaa tgaggctgcg ctcaaagcaa aactcacgga gatagaaaag tttaagttcc
      421 aaagtgaaga ctgcgaacct cgctaattga ttactggaac gattgtgtat gccagacagt
      481 gcaatactag ctctggcaag attaccatgt atttttgtcc agaatgtttt gcgaagagag
      541 caatatctat attgcaacct ttccctatcg aggaaggcga taaatatacg aaaactcaat
      601 gcccctcctg taaaaatgag tatctcatgg actacaattt caataatgat ttttgagtca
      661 cggtattcag atttttaaag aacatgccgg atactcaccc gtgtc
//
LOCUS       DAJTRM010000289          703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRM010000289 DAJTRM010000000
VERSION     DAJTRM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.299"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..410
                     /locus_tag="OO322_004301"
     CDS             <1..410
                     /locus_tag="OO322_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5908901.1"
                     /translation="HRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPR
                     KIFISASGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISA
                     FDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(412..638)
                     /locus_tag="OO322_004302"
                     /pseudo
     CDS             complement(412..638)
                     /locus_tag="OO322_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          179 a          171 c          156 g          197 t
ORIGIN      
        1 cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt aacagcaata ctttgtggtg
       61 gtacgtatcg tgccagaagt gatgcttttt acgatgccag taactcttcg ccattagact
      121 ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt gcataatcac tttggcgtca
      181 gctggtttaa ccctgaagat cttgccacta agcgtaaagt gatgaaccgt ggactacgga
      241 aaattttgct cgcccgccac gcgttgttcg atgaagtggc ctctgccagc ctcgcaccga
      301 tctctgcatt tgacgttctg attagcgatc gtccgttacc ggcagattat gttacgcact
      361 gccagaatgg ttctgtaaag atcattacac ctgattcaga agacgaatga cttactgaaa
      421 aaacaccaca atcttgttaa acatcgtcgg gttggactga ttacgttgca ctttcaccac
      481 atattccagc ttatctattt ggcttatcac ctactccaga cgctggtcat ccttgaccag
      541 tagccagata tggcttttgt cgcagtcctg aatcggcagg cagagaatgt cttcaacgtt
      601 aagagcgcga cgggcaaaag cccacaaagg tgagtcatta cacccggatg gtaaacaggc
      661 acttccttgt tgtagaagcc gttatattgt ccataatgtc tgg
//
LOCUS       DAJTRM010000290          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRM010000290 DAJTRM010000000
VERSION     DAJTRM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.300"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            33..>696
                     /gene="ospC4"
                     /locus_tag="OO322_004303"
     CDS             33..>696
                     /gene="ospC4"
                     /locus_tag="OO322_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5908902.1"
                     /translation="MIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEV
                     SRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRI
                     INFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAY
                     SLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYF
                     INN"
BASE COUNT          253 a           93 c          125 g          225 t
ORIGIN      
        1 atcatactgt agattttggc gccaatgcgt atatcattga tcatgactct ccatatggat
       61 atatgacatt aaccgatcac tttgataatg ctattccacc tgttttttac catgagcacc
      121 aatcattttt cttagataac tttaaagagg ttgtggatga agttagcagg tatgtgcatg
      181 gtaatcaggg gaaaactgat gttccaatat ttaacactaa agatatgagg cttggaattg
      241 ggttgcatct tattgatttt ataagaaaga gtaaagatca aggattcaga gagttttgtt
      301 ataacaaaaa tattgatcct gttagtctgg atagaattat aaactttgtg tttcagcttg
      361 agtatcatat accaagaatg ctaagtacag ataacttcaa aaaaataaaa ctcagagaca
      421 tatctttgga ggatgcgatt aaagcatcta attatgaaga gattaacaat aaagtgactg
      481 ataaaaaaat ggctcatcag gcccttgctt attctttagg caataaaaaa gctgatatag
      541 cactttattt actatctaaa tttaatttta caaaacaaga tgtcgcagag atggagaaaa
      601 tgaaaaacaa tagatattgt aatctgtatg atgttgaata cttacttagt aaagatggtg
      661 ccaactataa agtgctagaa tattttatca ataatg
//
LOCUS       DAJTRM010000291          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRM010000291 DAJTRM010000000
VERSION     DAJTRM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.301"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..673
                     /locus_tag="OO322_004304"
     rRNA            <1..673
                     /locus_tag="OO322_004304"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAJTRM010000292          658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRM010000292 DAJTRM010000000
VERSION     DAJTRM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.302"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(103..233)
                     /locus_tag="OO322_004305"
                     /pseudo
     CDS             complement(103..233)
                     /locus_tag="OO322_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(231..326)
                     /locus_tag="OO322_004306"
                     /pseudo
     CDS             complement(231..326)
                     /locus_tag="OO322_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          189 a          121 c          159 g          189 t
ORIGIN      
        1 tccttatgtt atattttggt aaaaaattta tttttttaca ttatagtcgg ctaaatgaag
       61 tttactgtaa aaaaacgata ctttttcggg tggcgcttcc aatcagtaga atatgtgttt
      121 ttcaatgaag gagccgatga cttttcgtgg atttccacgg aacgcgagaa gcagattgtt
      181 ttacgtgcca gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc aagatatagc
      241 atgtgctgga tgaagatccc ttttctggtc acctgtttat ctttcgtggc cgacggggtg
      301 acactgttaa aatgtaatga acgcatccca cacaattaaa cacggagtag tatgatggtt
      361 aaaagggtga aactggcagg tgtggagcct cgcagctttc cggcatcata ctgcccaata
      421 cgggatgcat aaagcgtcct cagactggag actccacaaa tgaaatatac accggttggc
      481 gttgatatgg caaaacatgt cattcagatt cacttcatca atgagcacat gtcagcgcca
      541 gtgatataag acggtaatgg ggatgcggac gatttcctgg actctcagaa gatgaaacat
      601 gtttactgaa gaacaaaaaa ttcgcgctat tgagctttat ttcaaatatg gtaagaag
//
LOCUS       DAJTRM010000293          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRM010000293 DAJTRM010000000
VERSION     DAJTRM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.303"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            52..>656
                     /locus_tag="OO322_004307"
     CDS             52..>656
                     /locus_tag="OO322_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5908903.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLP"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAJTRM010000294          652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRM010000294 DAJTRM010000000
VERSION     DAJTRM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.304"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            38..652
                     /locus_tag="OO322_004308"
                     /pseudo
     CDS             38..652
                     /locus_tag="OO322_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          142 a          172 c          189 g          149 t
ORIGIN      
        1 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg
       61 tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca
      121 gcgctatctc tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc
      181 tcaacccggt acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc
      241 aaccgcccgc attatgggcg ttggcctcaa cacgattttc cgccatttaa aaaactcagg
      301 ccgcagtcgg taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg
      361 gacgaacagt ggggatacgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat
      421 gacaggctcc ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg
      481 gggcgtctta tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg
      541 ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag
      601 cgaattgagc ggcataacct gaatctgagg cagcacctgg cacggctggg ac
//
LOCUS       DAJTRM010000295          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRM010000295 DAJTRM010000000
VERSION     DAJTRM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.305"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            52..303
                     /locus_tag="OO322_004309"
     CDS             52..303
                     /locus_tag="OO322_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908904.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            370..648
                     /locus_tag="OO322_004310"
                     /pseudo
     CDS             370..648
                     /locus_tag="OO322_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a          152 c          157 g          161 t
ORIGIN      
        1 aaaagggatg gaccccgaac aggggagtgc tacttatctg gaaggattct gatgatgaac
       61 atcgaagaac tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat
      121 ggaaatattg ttagtcatta ccgcattgtg tgtttgcaaa aaaatggggc tgcgttaatt
      181 aattttgtgg atgcccgagt gacggacgga tttatcttgc gcgacggtga gtttgtcact
      241 tcattacagg cattgaaaga gatcggaata aaagctggct tttctgcttt ttcagaagaa
      301 taaactcatc tacaatcttg cgcggggctg aactcccgct gagtaacacc gtgccaccgg
      361 agaaaaccga tggcacgcaa cgcaaaatat tacaatcatg ataattcgac cgttcttgcc
      421 cacacgcacg agcggtattc tcacgcattt aagtcagact ggtaccagca tccctcatgc
      481 actgaagaac aggccgaatg gcttattcag tgttaccgca ggcgcggatg cgaggttaaa
      541 aaagccctta gcctcgacta ccgtcactag ataatctccg tcaggctccc ttactccgaa
      601 cagccaccgc gtccgtcccg cacattccag caacggatct ggaggtaa
//
LOCUS       DAJTRM010000296          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRM010000296 DAJTRM010000000
VERSION     DAJTRM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.306"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>647)
                     /locus_tag="OO322_004311"
     CDS             complement(<1..>647)
                     /locus_tag="OO322_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5908905.1"
                     /translation="NYSASAPHKTGAGRGNPNVTRAHSRAEAVFLCVMHSSIQIMVGC
                     AGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMATIPALSHPDVTIE
                     NGRAVTTSVAVAEFFHKRHDNVLRAIANTECSPEFNALNFEDVTYTDTKGEKRPMYQI
                     TKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVV"
BASE COUNT          150 a          154 c          180 g          163 t
ORIGIN      
        1 acaaccagag tgcgaccatc acccggaatc atcttgtctg caggtggggt gttattctgg
       61 cgcagttcgg cctccatgcg gtcgaactca gcaatgtagg cttccttgaa tgcggcggct
      121 tttttgccag tgaagcccat caccaggaaa acgaagccgt ttttggtgat ttggtacatt
      181 gggcgttttt cgcctttggt gtcggtgtag gtgacgtcct caaaattgag ggcgttaaat
      241 tcaggtgagc attcagtatt tgcgatggca cgtaacacat tgtcgtgtcg tttgtggaag
      301 aactctgcaa ccgcaacaga cgtagtgaca gcgcgaccgt tttcgatggt tacgtcaggg
      361 tgagaaaggg cagggatagt agccatgatg gcatcctcga gtgataagtt aattaactca
      421 ccaccgaggt tttccacgac cataagggtg gtgagacgta caggggtgga aataccggtc
      481 actcgagaac ccggccagtc ttgcgactgc cctgcacatc ccaccataat ttgaatggag
      541 ctgtgcatta cgcataaaaa aaccgcttca gcgcggctat gcgctcgagt aacgttcggg
      601 tttccacgcc cggcccccgt tttatgaggt gcagatgcac tataatt
//
LOCUS       DAJTRM010000297          640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRM010000297 DAJTRM010000000
VERSION     DAJTRM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.307"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..561
                     /gene="ospC2"
                     /locus_tag="OO322_004312"
     CDS             <1..561
                     /gene="ospC2"
                     /locus_tag="OO322_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5908906.1"
                     /translation="QRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIR
                     LRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSKFNFTKQDI
                     AEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKANSGDTMLDN
                     AMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          237 a           80 c          125 g          198 t
ORIGIN      
        1 caaagattcc gagagttttg ttataataaa aacattgatc ctgttagtct ggatagaatt
       61 ataaactttg tgtttcagct tgagtatcat ataccaagaa tgctaagcac agataacttc
      121 aaaaagataa gactcagaga tatatctttg gaggatgcca ttaaagcatc caattatgaa
      181 gaaattaaca ataaggtgac tgataaaaaa atggctcatc aagctcttgc ttattctcta
      241 ggcaatgcaa aatctgatat ggcactttat ttactatcta aatttaattt tacaaaacaa
      301 gatatcgcag agatggagaa aatgaacaac aatatgtatt gtgagctgta tgatgttgag
      361 tatttactta gtgaagatag tgccaactat aaagtgctag aatattttat tagtaatgga
      421 ttggttgatg taaacaaaag atttcaaaaa gcaaatagtg gggacactat gcttgacaat
      481 gcaatgaaaa gcaaagattc aaaaacaatt gattttttat taaaaaatgg agcggtatca
      541 ggcaaacgat ttgggaggtg acctaaaata tatttaggtt gtgccctgaa tctgacgttg
      601 tcatttttgt tatattgatc gatatagggg atggtgcaga
//
LOCUS       DAJTRM010000298          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRM010000298 DAJTRM010000000
VERSION     DAJTRM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.308"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(40..115)
                     /locus_tag="OO322_004313"
     tRNA            complement(40..115)
                     /locus_tag="OO322_004313"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Ile,seq:cat)
     gene            complement(292..>638)
                     /locus_tag="OO322_004314"
     CDS             complement(292..>638)
                     /locus_tag="OO322_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5908907.1"
                     /translation="EYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKA
                     ELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQK
                     AAFSKQSIAKVD"
BASE COUNT          155 a          162 c          129 g          192 t
ORIGIN      
        1 tagaaggttg atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg
       61 aaccagcgac ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg
      121 cagaataata accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac
      181 gtcctgtgcc atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac
      241 catcgcgtac aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac
      301 ttttgcaata ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa
      361 caataaagcc tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt
      421 aaatgttttg gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc
      481 ataaccttcc ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt
      541 tttcaggcgt tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc
      601 ttcccacgcc cagtgggtta tggtgatctg atactccc
//
LOCUS       DAJTRM010000299          636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRM010000299 DAJTRM010000000
VERSION     DAJTRM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.309"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>636
                     /locus_tag="OO322_004315"
     CDS             <1..>636
                     /locus_tag="OO322_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908908.1"
                     /translation="QDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGK
                     ACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRY
                     VDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEP
                     LAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLIST"
BASE COUNT          165 a          133 c          182 g          156 t
ORIGIN      
        1 ccaggacact atcaacgctt tctacggcaa gcagcagtat ctgtttaatc caccgtggga
       61 atctctggca gataaattcc agttccgtga ggcagagttg acgctggtca atggtgtgca
      121 cggtcacgga aaggcatgcc cactgaatga gcctattctt ttagctgatg ggacatggac
      181 tactcacggg aatgtaaaaa ttggcgatca ggtggcgtca gtagacggca atccgtcaac
      241 tgtcactggg atattcccgc agggtgttag agatgtttac cgagtcacat ttgaagatgg
      301 tcgttatgtt gattgcgcag gcgatcacct atgggaggtc actagtcgtg gattcacgaa
      361 aggcgagaaa cgccgcgtga tagatacctt cgggttgaag cggttgagtg aaacgaagag
      421 gcacaaaaat ggcgttagga ttcctgaaat aactggtgac tttggcgacc actcagagcc
      481 attagcatgg gttatcggct cccttctcgg ggatggtagt cttagcaatg ggagcgtgaa
      541 gttttcaaac gtcgagccat acatgatcga gcgtatgaag gctgaactgc ctgattacaa
      601 cttctctgga gatggtaagg actggctgat atcaac
//
LOCUS       DAJTRM010000300          622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRM010000300 DAJTRM010000000
VERSION     DAJTRM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.310"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..185
                     /locus_tag="OO322_004316"
     CDS             <1..185
                     /locus_tag="OO322_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5908909.1"
                     /translation="LPQHLFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRY
                     MAETIAHHQVDIDLED"
     gene            191..>622
                     /locus_tag="OO322_004317"
     CDS             191..>622
                     /locus_tag="OO322_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5908910.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTD"
BASE COUNT          172 a          147 c          158 g          145 t
ORIGIN      
        1 gtctgcctca gcacctgttt gaatcgctct gcatcgacca tttgcaacgc cacggtgcca
       61 ataaacaggc aatcagccac gcatttgatg atgatgttga gtttcaggag cgcatggcag
      121 aacacatccg gtacatggct gaaaccattg cccaccacca ggttgatatt gatttggagg
      181 actaaaatct atggcgcttt ttcaacgagc aataaacaca caggcctatc taaaagcagg
      241 aattatgggg tttgctggcg acggcaaaac ctacacagca agcgaactgg ctattggtct
      301 ggtaatgctt atgcggcaac ggggaattga agctggtaac cgcccggtaa tgtttctgga
      361 cacagaaaca ggctctgact gggttaaacc ccgctttgat gcggaaaata tcgaattatt
      421 tacggcaaaa acccgctcgt ttgttgatct gcttgaagcc attaatgaag cggaaagcag
      481 cggttccgta atgatcatcg actccatcag tcacttctgg accggattat gcgacgagta
      541 tgccagacgc cgaaaccgta aacgaggact ggaattttca gactgggcgt ggctcaaaca
      601 ggagtggcga cgctttaccg at
//
LOCUS       DAJTRM010000301          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRM010000301 DAJTRM010000000
VERSION     DAJTRM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.311"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO322_004318"
     CDS             complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO322_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5908911.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..>615)
                     /locus_tag="OO322_004319"
     CDS             complement(332..>615)
                     /locus_tag="OO322_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HCR5908912.1"
                     /translation="IGLDTPLERLLLQALQGGGGLEPGLADRLLAQTQEALSRQEMLG
                     APPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRHIRMTATIGGK"
BASE COUNT          151 a          195 c          152 g          117 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataa
//
LOCUS       DAJTRM010000302          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRM010000302 DAJTRM010000000
VERSION     DAJTRM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.312"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            67..132
                     /locus_tag="OO322_004320"
                     /pseudo
     CDS             67..132
                     /locus_tag="OO322_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            360..>605
                     /locus_tag="OO322_004321"
     CDS             360..>605
                     /locus_tag="OO322_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5908913.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          186 a          125 c          142 g          152 t
ORIGIN      
        1 aaaactacct ggttcgcaaa actgcgtcta aagttaaact gggacctcgc gagcaagggt
       61 gagacgatgg cgctttacac aattggtgaa gtggcgttgc tttgtgatat taatcctgtc
      121 acgttacgcg cgtgacgcaa catttttaaa aatcgacgaa gatcgtccag gagcgccgtt
      181 tttcaagggt tggatagatg attcgtttac catgtcgacg aacttcgacg aatttcgccg
      241 ataaaaagtg tcccatacat gccccattga tgccccataa acaatatcag gaattgacac
      301 tggttatcca tacagataaa aataacacct gtacataact acagttacag gagggtgtta
      361 tgcgtgttga gatctgtatc gctaaagaaa aaatcactaa aatgccaaac ggtgctgtgg
      421 atgcgttaaa ggaagaatta acccgacgca tcagtaaacg ttatgacgat gtagaggtga
      481 tcgtaaaagc caccagcaac gatggccttt ctgttacacg caccgcagat aaggattctg
      541 caaaaacatt tgttcaggag actctgaaag atacctggga gtctgctgac gagtggtttg
      601 ttcac
//
LOCUS       DAJTRM010000303          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRM010000303 DAJTRM010000000
VERSION     DAJTRM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.313"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            18..>605
                     /locus_tag="OO322_004322"
     CDS             18..>605
                     /locus_tag="OO322_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5908914.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRL"
BASE COUNT          130 a          162 c          175 g          138 t
ORIGIN      
        1 gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct
       61 ggcttaactg gctggagaaa caaccggaag gggcagtccg tccggtggta attgaatcag
      121 tgacaaaaat catggcctgc gggaccacac tgatgggcta cacgcagtgg tgttgttcat
      181 caccggactg ttgccataca aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc
      241 actgcggggt gaaggctggc gcacagtgga tacagtatct gctgagcctg gtcccctact
      301 gcccgtggca gcatattgtg ttcacacttc cctgccagta ctggtccctg gtgttccaca
      361 accggtggtt actggcagag atgagccgca ttgcagcgga tgtgatactg gaaatctgcc
      421 atcaggcaga tgtggagccg gggatattca cggtgatcca cacatggggg cgtgaccagc
      481 agtggcatcc gcatatccat ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct
      541 ggaaaaatct tcatttttac gcccgtaagg tgatgagcat gtggcgctac cggataacac
      601 ggttg
//
LOCUS       DAJTRM010000304          601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRM010000304 DAJTRM010000000
VERSION     DAJTRM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.314"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            27..101
                     /gene="yoaJ"
                     /locus_tag="OO322_004323"
     CDS             27..101
                     /gene="yoaJ"
                     /locus_tag="OO322_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5908915.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(262..453)
                     /locus_tag="OO322_004324"
                     /pseudo
     CDS             complement(262..453)
                     /locus_tag="OO322_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a          145 c          141 g          166 t
ORIGIN      
        1 tgctatgctg ccccgggagc ataaagatga aaaaaacaac gattattatg atgggtgtgg
       61 cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacctc taaaaaatag
      121 caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt taacttttaa tcttcctgta
      181 gataaatagc acgacaatcg caccaataac ggctgaagcg gcgcacgaaa aacgctttcg
      241 cgtttttcgt gcgccgcttc acccctggtt cagcatttcg tctgcagtct gaaccgcagg
      301 caaccatttt tcagcaagtc tgcgctggga atcgctggct ttatcccatt tgctccacag
      361 tgcctcacga tcgtaatcgt gcgcttccag cgtggggcgg gcgatttcaa aataccccta
      421 gcttgtttct atttgctcag attttaataa caactcagca cgtgctgccg ctggtagaac
      481 tgatatatga aattccaaag cctttgaacc cgtacgcttg cgacgtagtt ctggatgatt
      541 ggctgcaagc ttttcaagat tgcggcgaat actcacctca tgtttaggaa agcatgacat
      601 t
//
LOCUS       DAJTRM010000305          598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRM010000305 DAJTRM010000000
VERSION     DAJTRM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.315"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>598)
                     /locus_tag="OO322_004325"
     CDS             complement(<1..>598)
                     /locus_tag="OO322_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5908916.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQL"
BASE COUNT          160 a          145 c          107 g          186 t
ORIGIN      
        1 tagttgtgcc tgtgtacttt ctgaaatagc tttccagcca cctttagcgg tgatttgttt
       61 attcagttcc tgagtgacct tctgaagagt atcggcagat tttaaagccc ccttcttcat
      121 agcatcaaat aattcctgtt cagtctttcc ggttgcttta gcgaatacct gaaccatacc
      181 aggaacagct tcagctagtt gccccttaag ctcttcagcc ataatctgac ccttaccagc
      241 catctgtgat aatgctgtca gtgctcgctt aatctcttct tcacttcgcc ccatcagacg
      301 agaatagcca atcatatttc taaaaagttc ttcagaacct tgaatgccta gagactctct
      361 agctgaagca ccgaaaccag caaactgtga ttgcagttca gcgatatcta aacccgttgt
      421 ttgagctaat gactttgtga aatcttttac ttgatttgca tctttagaaa atacgaaatc
      481 aattgaacgt tcagcacttg cacgttgaag gccaacttcc attactcgct gataagcgga
      541 tagtacagcc tgaaagctga taagtacacc tgctgtttta actgctgctg aacttaaa
//
LOCUS       DAJTRM010000306          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRM010000306 DAJTRM010000000
VERSION     DAJTRM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.316"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..291
                     /locus_tag="OO322_004326"
     CDS             1..291
                     /locus_tag="OO322_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5908917.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            288..>581
                     /locus_tag="OO322_004327"
     CDS             288..>581
                     /locus_tag="OO322_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5908918.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTD"
BASE COUNT          175 a          124 c          166 g          116 t
ORIGIN      
        1 atggtggatt tacgtaaagc ggcgcggggg cagatgtgca ccgtcagaat tcctggctac
       61 tgcaatcaca atcccgaaac ttctgtgctg gcgcattaca ggctggcggg gacgtgcgga
      121 acagcgacaa aaccacacga tatgcaggca gcgattgcct gtagctcatg ccacgattta
      181 atcgacgggc gggtaaaaac cagcgattac accaaagaag aattacgcct gatgcatgca
      241 gaaggtgttt ttcgcacaca agaaatctgg agaaaggaag gttatttatg atttacccaa
      301 caaatacagg caaaagcggg gaacaccttc gtctcaccac gctggaaagt gtctggattc
      361 agggaaaact gcgcatgtgg gggcgctggt cgtatattgg cggcggtaag acgggaaata
      421 tgtttaacca gttgctggca tccaaaaaat tgacgaaaac agccgtcaat gaagccctgc
      481 gcaggatgaa aaaagcggga atagagaaac ctgagctgga agcgtttttg cgagagatga
      541 tcaatggcaa gcaaaagacc tggctggcgc attgtactga t
//
LOCUS       DAJTRM010000307          572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRM010000307 DAJTRM010000000
VERSION     DAJTRM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.317"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>572
                     /locus_tag="OO322_004328"
     CDS             <1..>572
                     /locus_tag="OO322_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5908919.1"
                     /translation="TTLEIRKNLLTYLPDFPPMLKVIHAQFNQLESLPALPETLEELN
                     VGDNKIKELPYLPETLTHLRIHNNRLHILPLLPPELKLLIVSGNRLDSTPPFPDKLEG
                     LALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRT
                     LQQITTGPDYSGPQIFFSMGNSATISAPEH"
BASE COUNT          187 a          134 c           94 g          157 t
ORIGIN      
        1 taacaacact ggaaataaga aaaaacctat taacatatct ccctgatttc ccaccaatgc
       61 ttaaggtaat acatgctcaa tttaatcaac tggaaagctt acctgcctta cccgagacgt
      121 tagaagagct taatgtgggt gataacaaga taaaagaatt accatatctt cctgaaactc
      181 taactcattt acggatccat aataaccgat tgcatattct gccactattg ccaccggagc
      241 taaaattact gatagtttct ggaaacagat tagacagcac cccccccttt ccagataagc
      301 ttgaagggct ggctctggct aataatttta tagaacaact accggaatta ccttttagta
      361 tgaacagggc tgtgctaatg aataataatc tgacaacact tccggaaagt gtcctgagat
      421 tagctcagaa tgccttcgta aatgttgcag gtaatccatt gtctggccat accatgcgta
      481 cactacaaca aataaccacc ggaccagatt attctggtcc tcaaatattt ttctctatgg
      541 gaaattctgc cacaatttcc gctccagaac ac
//
LOCUS       DAJTRM010000308          570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRM010000308 DAJTRM010000000
VERSION     DAJTRM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.318"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            106..>570
                     /gene="curA"
                     /locus_tag="OO322_004329"
     CDS             106..>570
                     /gene="curA"
                     /locus_tag="OO322_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5908920.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVV"
BASE COUNT          127 a          125 c          181 g          137 t
ORIGIN      
        1 tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca atcccgtgag
       61 cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg gcaacaaaag
      121 cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt tccggagaat
      181 ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt actgcgcaca
      241 gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc atcttattca
      301 ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt cgtggagtcg
      361 aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg gcaggactat
      421 gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa tccatcgtgg
      481 tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct ggatatcggt
      541 cagcctaaag agggcgaaac gttggtggta
//
LOCUS       DAJTRM010000309          567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRM010000309 DAJTRM010000000
VERSION     DAJTRM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.319"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..567
                     /locus_tag="OO322_004330"
                     /pseudo
     CDS             1..567
                     /locus_tag="OO322_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          135 a          143 c          168 g          121 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gctcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgc
//
LOCUS       DAJTRM010000310          560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRM010000310 DAJTRM010000000
VERSION     DAJTRM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.320"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..560
                     /locus_tag="OO322_004331"
                     /pseudo
     CDS             1..560
                     /locus_tag="OO322_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          159 a          142 c          149 g          110 t
ORIGIN      
        1 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
       61 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      121 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      181 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      241 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      301 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      361 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      421 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      481 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      541 ggtgcggtga tgtgacctat
//
LOCUS       DAJTRM010000311          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRM010000311 DAJTRM010000000
VERSION     DAJTRM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.321"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>544
                     /locus_tag="OO322_004332"
     CDS             <1..>544
                     /locus_tag="OO322_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5908921.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAEL"
BASE COUNT          150 a          123 c          126 g          145 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 atta
//
LOCUS       DAJTRM010000312          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRM010000312 DAJTRM010000000
VERSION     DAJTRM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.322"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          158 a          132 c          113 g          135 t
ORIGIN      
        1 agatcgcgta gcattcttaa ttcatgaggg actttacccg ccagcaatct gagtagcgaa
       61 agctgttcat ccccaatgtt tttgctggcg ggtccttttt tctacggtcc tcctccccgg
      121 aggggggcat ttcattattt ttctaatcgt tactggtgag aaggcggaag aaaaagccag
      181 ttcttcgccc ctaaataaca gcatgattcc agtccatgca aaatctgatt tccagctcac
      241 acataccgag cgctgtacaa aaaaccgcca gccaaggcag ttaagagtgt gttcccgggg
      301 tttgcttaga atattttgta agttgtccga agagatattt acaacaccag aatgatgaat
      361 catcagtccc ctgccagagg ttcactacgt aacctgaccg acaatgcacc attctggtgc
      421 tgaaaaaaga gcactggaac tgcaacaaaa caacatcaca gaacaggaga tggtaaggag
      481 tcagacattc ccacacaata ttgtgtcagt gcaaataaca accttcgtct caaatctc
//
LOCUS       DAJTRM010000313          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRM010000313 DAJTRM010000000
VERSION     DAJTRM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.323"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..508
                     /locus_tag="OO322_004333"
     CDS             <1..508
                     /locus_tag="OO322_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR5908922.1"
                     /translation="SMAHKLGSQFHIPHGLANALLICNVIRYNANDNPTKQTAFSQYD
                     RPQARRRYAEIADHLGLSAPGDRTAAKIEKLLAWLETLKAELGIPKSIREAGVQEADF
                     LANVDKLSEDAFDDQCTGANPRYPLISELKQILLDTYYGRDYVEDETAAKKEAAPAKA
                     EKKAKKSA"
BASE COUNT          146 a          141 c          136 g          111 t
ORIGIN      
        1 ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg caaacgccct
       61 gctgatttgt aacgttattc gctacaatgc gaacgacaac ccgaccaagc agactgcatt
      121 cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgccg accacctggg
      181 tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg catggctgga
      241 aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg ttcaggaagc
      301 agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg
      361 cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg atacctacta
      421 cggtcgtgat tatgtagaag atgaaactgc agcgaaaaaa gaagccgctc cggctaaagc
      481 tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaata taaa
//
LOCUS       DAJTRM010000314          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRM010000314 DAJTRM010000000
VERSION     DAJTRM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.324"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..527)
                     /locus_tag="OO322_004334"
                     /pseudo
     CDS             complement(1..527)
                     /locus_tag="OO322_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          184 a          125 c          124 g           94 t
ORIGIN      
        1 aaacgcaata aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa
       61 aaaggccaga tcaaacgcgc tcacagcttg gcgtggtccg ggcaaattgc gcaacacggc
      121 aaagtaccag aagaagattt gcaatattac ggaagatctc aatataaatt gtggaaagct
      181 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
      241 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
      301 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
      361 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
      421 tttgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
      481 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggtt
//
LOCUS       DAJTRM010000315          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRM010000315 DAJTRM010000000
VERSION     DAJTRM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.325"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..341
                     /locus_tag="OO322_004335"
     CDS             <1..341
                     /locus_tag="OO322_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5908923.1"
                     /translation="CTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSY
                     QSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRRLSES
                     VPCMPHKKAG"
     gene            362..>520
                     /locus_tag="OO322_004336"
     CDS             362..>520
                     /locus_tag="OO322_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR5908924.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQV"
BASE COUNT          159 a          122 c          123 g          116 t
ORIGIN      
        1 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
       61 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
      121 acagaaaatc gtatcaatcc aggcatacct atgcatgctg gtcacttgcc gcaggagcta
      181 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
      241 acggagcatg gatgaaagac aacaatcacg aacagataga acttcttaac agaagactat
      301 ctgaaagtgt cccatgcatg ccccataaga aagcggggta aaataaaaac ttgcaaaatc
      361 aattggttta cctttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct
      421 gaaaccgcaa cggacagacg gcggtcatcg gctgttcaac gatgccgata ttgaccggat
      481 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc
//
LOCUS       DAJTRM010000316          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRM010000316 DAJTRM010000000
VERSION     DAJTRM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.326"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>520)
                     /locus_tag="OO322_004337"
     CDS             complement(<1..>520)
                     /locus_tag="OO322_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5908925.1"
                     /translation="KRYEVVSGKVWNGERELDALVLPYKRDGELLQVKRISTERPDGK
                     KVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQ
                     QWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMN
                     GVTEDEIWQYIGT"
BASE COUNT          122 a          167 c          104 g          127 t
ORIGIN      
        1 gtgccgatgt actgccagat ttcatcctcg gtaacaccgt tcatcaggca ttcgttgatg
       61 tctttgtacg gcagagtaac aagacggcaa cgatgttcac cgagtcggct tacgatttcc
      121 cttgcggctt cacgaccaac atcatcaacg tccatcgaga tgaatatttc ctcaaacctg
      181 tcgaggttgt gatactcaaa ctcaatccac tgttgcttag cgcctttccc gccaccaaac
      241 ggcacggata acgccgagat gccgtattgc gcatagctca tacaatcaat ttcgccttcg
      301 caaagcacaa ccaccctcac gccagcgtcc agagcctgcc atccgaacag gcaaggttcg
      361 caatcacctt ctgccataat gactttcttc ccgtccgggc gctcagtgct gattcgcttg
      421 acctgcaaca actcaccatc gcgtttgtac ggaagcacca gagcatccag ttctcgttct
      481 ccattccaca ccttgccgct gacaacctcg tagcgcttta
//
LOCUS       DAJTRM010000317          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRM010000317 DAJTRM010000000
VERSION     DAJTRM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.327"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            214..>518
                     /locus_tag="OO322_004338"
     CDS             214..>518
                     /locus_tag="OO322_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5908926.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          174 a          112 c           87 g          145 t
ORIGIN      
        1 cagaaagcgc ataaaaataa caacataaaa caccttgcca ccacccaaga attctctatt
       61 acattgtttt taatgaaaaa atatcattcc aacaatatct cacgataaat aacattagga
      121 ttttgttatt taaacacgag tcctttgcac ttgcttactt tatcgataaa tcctactttt
      181 ttaatgcgat ccaatcattt taaggagttt aaaatggata agaagcaagt aacggattta
      241 aggtcggaac tactcgattc acgttttggt gcgaagtcta tttccactat cgcagaatca
      301 aaacgttttc cgctgcacga aatgcgcgac gatgtcgcat ttcagattat caatgatgaa
      361 ttatatcttg atggcaacgc tcgtcagaac ctggccactt tctgccagac ctgggacgac
      421 gaaaacgtcc acaagttgat ggatttatcc attaacaaaa actggatcga caaagaagaa
      481 tatccgcaat ccgcagccat cgacctgcgt tgcgtaaa
//
LOCUS       DAJTRM010000318          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRM010000318 DAJTRM010000000
VERSION     DAJTRM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.328"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            1..491
                     /gene="tcyP"
                     /locus_tag="OO322_004339"
                     /pseudo
     CDS             1..491
                     /gene="tcyP"
                     /locus_tag="OO322_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT           92 a          144 c          151 g          129 t
ORIGIN      
        1 acgtttgcct ttaccagccg ttccagtgct gcgtctatcc cactgaatgt ggaagcacaa
       61 acgcgtcgtc tgggcgttcc tgaatccatc gccagtttcg ccgcctcttt cggtgcaacc
      121 attggtcaga acggctgcgc cggtttgtat ccggcaatgc tgcggtgatg gttgcgccta
      181 cggttggcat taacccgctg gacccgatgt ggattgcgac gctggtcggt attgttaccg
      241 ttagttccgc aggcgttgcc ggtgtcggtg gtggtgcaac tttcgccgca ctgattgtgc
      301 tgcctgcgat gggcctgcca gtaaccctgg tggcgctgtt aatctccgtt gaaccgctta
      361 tcgacatggg ccgtacggcg ctaaacgtta gtggctcgat gacagctggc acgctgacca
      421 gccagtggct gaagcaaacc gataaagcca ttctggatag cgaagacgac gccgaactgg
      481 cacaccgtta attcttatgc tggcaacggt ccgttt
//
LOCUS       DAJTRM010000319          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRM010000319 DAJTRM010000000
VERSION     DAJTRM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.329"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>514)
                     /locus_tag="OO322_004340"
     CDS             complement(<1..>514)
                     /locus_tag="OO322_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5908927.1"
                     /translation="RPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLF
                     SSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQN
                     EPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVD
                     PQAKDISHLLA"
BASE COUNT          131 a          125 c          128 g          130 t
ORIGIN      
        1 agccagcaaa tgactgatat ctttagcctg aggatcaacc gagaggcgag aagaaacgct
       61 gtatgcccca gatgcctgtt cacgaatatt aacgcgtaga tcttttgcca gtgcgacgtt
      121 aaaagcatcg agcgccatac gcgtcgccag attaacaggt gtccgggaat cataacgctt
      181 ccactgtgaa acctgtgcca caggttcatt ttgttctttt acagtaaccg atgcgttgtc
      241 cgtcgcgcga gttaatggtt tacctgcggc taatggcgaa tcagagtgtt tgattgagcc
      301 taagtaacgc gtaattaacg ccacgagttt gtcttctgcg acattaccga caatgacaaa
      361 cgtgatatcc gctggagatg aaaacaactg gcgatcggca gccagcgcat ctgcggcagt
      421 aaactgtgca atctgatttt cttgcagtaa tttcgtgcgg tcatcagcat agcgcgtctc
      481 atacatcagc tgggcgaatt tctccgctgg acgc
//
LOCUS       DAJTRM010000320          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRM010000320 DAJTRM010000000
VERSION     DAJTRM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.330"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..227)
                     /locus_tag="OO322_004341"
     CDS             complement(<1..227)
                     /locus_tag="OO322_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5908928.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKG"
BASE COUNT          168 a          123 c           97 g          125 t
ORIGIN      
        1 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
       61 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
      121 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
      181 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
      241 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
      301 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
      361 ttagtgtaaa taatagacgc caaaatgtgg caaagcacat agaacaccga acactacgtt
      421 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
      481 gaagaataaa aaataaccaa aacgttgaaa gga
//
LOCUS       DAJTRM010000321          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRM010000321 DAJTRM010000000
VERSION     DAJTRM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.331"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..264
                     /locus_tag="OO322_004342"
     CDS             <1..264
                     /locus_tag="OO322_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5908929.1"
                     /translation="AKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRS
                     KDARANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            278..>510
                     /locus_tag="OO322_004343"
     CDS             278..>510
                     /locus_tag="OO322_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5908930.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQ"
BASE COUNT          157 a          136 c          123 g           94 t
ORIGIN      
        1 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
       61 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
      121 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
      181 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
      241 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
      301 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
      361 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
      421 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
      481 acgcaaacgc cagcgtgagc ttgagcagca
//
LOCUS       DAJTRM010000322          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRM010000322 DAJTRM010000000
VERSION     DAJTRM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.332"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>501)
                     /gene="ssuC"
                     /locus_tag="OO322_004344"
     CDS             complement(<1..>501)
                     /gene="ssuC"
                     /locus_tag="OO322_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5908931.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVV"
BASE COUNT          143 a          153 c          119 g           86 t
ORIGIN      
        1 cgactaccac cacgtctgtt tgcaaaaact ctcgcgcatt catcgccaga taaccaatgc
       61 cggaattggc agaaatggtt tcggcaacaa tcagcgtcag ccacatcagg cccaacgcaa
      121 aacgcacgcc gaccataatt gagggcaggg catcgggcag gatcacatgg ataaacagcg
      181 gtatgccgga taatccatag ctacgcgcca tctccaccag cccgcgatcg atattacgga
      241 tgccatgcca ggtgttgata taaatgggga acagcgtacc gagcgccacc agaaagattt
      301 tcgcggattc atcaatgcca aaccacaaaa tcaccagcgg gatcagcgcc agatgcggca
      361 cgttgcgcaa catctgaatt gaggtatcca gcagcctttc tccccagcgc gacagtccgc
      421 taatcagtcc cagaatcagg cccagcgatc cgccaattga aaagccaatc agcgcccgcc
      481 aggaactgat cgccagatgc t
//
LOCUS       DAJTRM010000323          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRM010000323 DAJTRM010000000
VERSION     DAJTRM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.333"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..207)
                     /gene="ospI"
                     /locus_tag="OO322_004345"
     CDS             complement(<1..207)
                     /gene="ospI"
                     /locus_tag="OO322_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5908932.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHA"
     gene            complement(360..489)
                     /locus_tag="OO322_004346"
                     /pseudo
     CDS             complement(360..489)
                     /locus_tag="OO322_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          145 a          126 c           77 g          141 t
ORIGIN      
        1 agcatgaagc gcacaccctg aacagttacc atccccacca gacggattta tcattttctg
       61 taaagtcgct gccaattcag aatctttatc catatcacca agacttttag agtttaaaga
      121 ttccagcaat tttttaacat ttacatttcc ctcctctgta tgtgcctcat atccagcagt
      181 tccctgtaac gacaccccat taatcatatt gtatcctgac agtatatata ctcactatat
      241 tttcttcata acaaatatcc ctctgatatg acaacaaaaa acggagccgg attcctgttt
      301 ttgtgaactg cagctattcg tgtctaaaaa gtgatccact taccaccaac ggcctaatat
      361 atacagaacg gagtaaacag gactaacagt tcccgacagg tctcatcatt ccgtggcccg
      421 aaagtgtaag ccaacacacc gccagtttta gtgttgtacg aataccagag ccagtgttgt
      481 tgcagcctt
//
LOCUS       DAJTRM010000324          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRM010000324 DAJTRM010000000
VERSION     DAJTRM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.334"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          167 a           92 c          105 g          124 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 gggggggg
//
LOCUS       DAJTRM010000325          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTRM010000325 DAJTRM010000000
VERSION     DAJTRM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.335"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..150
                     /locus_tag="OO322_004347"
     CDS             <1..150
                     /locus_tag="OO322_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HCR5908933.1"
                     /translation="ERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGINGK
                     WVRKF"
     gene            complement(322..>485)
                     /locus_tag="OO322_004348"
     CDS             complement(322..>485)
                     /locus_tag="OO322_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5908934.1"
                     /translation="RLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMT
                     PGEYRRKFR"
BASE COUNT          159 a          105 c          109 g          112 t
ORIGIN      
        1 gaacgtaaag cgagttacca acaacgggca aagcaaaata atgtgtctgt agatgatatc
       61 gcgaaactcg ccgggcaaaa gctcgtagct cgggccaaac cgggggaata tgtacaaggg
      121 ataaacggta aatgggtgcg aaagttttaa tagcaaaacc gcaatatcgg gataacgcta
      181 caggagaaag cgattgatct attttcctga aacaaggtga atattcaaaa actcctgtca
      241 aattgccttt cgccctgaaa aatgcatagt caaatatctg ttttaactaa ttggcgttgc
      301 agtacatgca acgccaatta gttagcggaa tttacgtcga tactcgcctg gcgtcatccc
      361 aaagcgttgc ttaaataccg ttgaaaaatg actctggtca gaaaatcccc aatgaaagcc
      421 gatgcctgcc agtttttcat catctgcggc atggcgaatc gcatctgcac aaaaatcgag
      481 acgac
//
LOCUS       DAJTRM010000326          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRM010000326 DAJTRM010000000
VERSION     DAJTRM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.336"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(74..>471)
                     /locus_tag="OO322_004349"
     CDS             complement(74..>471)
                     /locus_tag="OO322_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HCR5908935.1"
                     /translation="EKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKD
                     VAFVSSRMSGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDN
                     GQAELILRNGLTVPVSRRYLKSLKEAIGL"
BASE COUNT          116 a          126 c          105 g          124 t
ORIGIN      
        1 gctgcgcaga atatcgttac ttatcatgag gccttcaggt gttgatgagg caaaaagcca
       61 ttttagcagt cttttacagg ccaatcgcct cttttaagct tttcagataa cggcggctga
      121 ccggcacggt taagccatta cgcaaaatca actcggcctg gccgttatct tccagacgaa
      181 tctcctgtaa atgcgcgagg ttaaccagat actgacgatg gcagcgcagt agtggtgtac
      241 gactttccaa ggtacgtaat gtcaattcgg taaagccctc tttcccttcg tggctggtaa
      301 cgtagacacc gctcatccga ctgctgacaa atgccacatc tttcatttgc agcaaataaa
      361 tccgactatg ccccgtacaa gggataaatt tcagcgcctg ttgattttcc ggtaacagcg
      421 aaacatcctg cttgctgcgc tcctgacgca atcgcgccag cgttttctcc a
//
LOCUS       DAJTRM010000327          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRM010000327 DAJTRM010000000
VERSION     DAJTRM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.337"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..333
                     /locus_tag="OO322_004350"
     CDS             <1..333
                     /locus_tag="OO322_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908936.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIY
                     DSFCRGVA"
     gene            330..>467
                     /locus_tag="OO322_004351"
     CDS             330..>467
                     /locus_tag="OO322_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908937.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EH"
BASE COUNT          119 a          131 c          126 g           91 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaatct ggtgagcttc gacaaccccc cccagcatca tccactctcc
      301 atctacgact cattctgcag aggagtggcg tgatgatgga actgcaacat caacgactga
      361 tggcgctcgc cgggcagttg caactggaaa gccttataag cgcagcgcct gcgctgtcac
      421 aacaggcagt agaccaggaa tggagttata tggacttcct ggagcat
//
LOCUS       DAJTRM010000328          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRM010000328 DAJTRM010000000
VERSION     DAJTRM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.338"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..221
                     /locus_tag="OO322_004352"
     CDS             <1..221
                     /locus_tag="OO322_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5908938.1"
                     /translation="GNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRTDDALWRRTKQ
                     GMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT          130 a          108 c          120 g          108 t
ORIGIN      
        1 tcggcaatgc gggaacggta agcgatctcg gggaagattt cggtcatgag ttctacgaag
       61 cggaactgaa atacctggtg gatcacgaat gggtccgccg caccgacgat gccctgtggc
      121 gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca atctcgtgtg agtcagtggc
      181 tggtggaata tacgcagcag aagttgtcgc tggcgtcgta aattaacgta aggtgatcag
      241 gtcagatttc aatctggcct gagactgatg acaaacacaa aactgcctga tgcgctacgc
      301 ttatcaggcc tacgtggttt atgcaatata ttgaatttgc atggtcttgt aggccagata
      361 agacgttcac gtcgcatccg gcatgaacaa agcgcacttt gtcaaaaatc taacctactt
      421 tttaattcag gaaattaccg caaagcccac gccatcatac ccaacg
//
LOCUS       DAJTRM010000329          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRM010000329 DAJTRM010000000
VERSION     DAJTRM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.339"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            35..452
                     /locus_tag="OO322_004353"
                     /pseudo
     CDS             35..452
                     /locus_tag="OO322_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          116 c          121 g          114 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc tt
//
LOCUS       DAJTRM010000330          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRM010000330 DAJTRM010000000
VERSION     DAJTRM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.340"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            101..>450
                     /locus_tag="OO322_004354"
     CDS             101..>450
                     /locus_tag="OO322_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5908939.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          111 a          125 c          116 g           98 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat
//
LOCUS       DAJTRM010000331          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRM010000331 DAJTRM010000000
VERSION     DAJTRM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.341"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>443
                     /locus_tag="OO322_004355"
     CDS             <1..>443
                     /locus_tag="OO322_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5908940.1"
                     /translation="TQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQ
                     RLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLP
                     IGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVA"
BASE COUNT          124 a          104 c          122 g           93 t
ORIGIN      
        1 acgcagatac tgaaagaccg cgagaagata gccggagagc atattccaat cgttccagtg
       61 tatggcgaat ggtcattcgc tggtgacaag gagtgctacg agggtgtggt aaggctgacg
      121 aaagacggtc aacgccttcg taacatgatt atgtcgttca acgccgatat tgttgctcgg
      181 tcaccgaaga agaaaccgac cttcttccct gagcaaatcg aaggctacga atacatgtac
      241 ggcggaaatg atgactatcc gtactatctg cagaacagga ctgatgaaaa cggtaacgac
      301 ctgccgattg gtccaatctc ctacatggaa aaccctgaag tgccgcaagc caacgcttac
      361 atgcttgagg ctgccaccaa cgcagtgaaa gaagtggcta gtcttggcgt ggatgcgcag
      421 gcggcaaatg gtcaggtcgc ttt
//
LOCUS       DAJTRM010000332          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRM010000332 DAJTRM010000000
VERSION     DAJTRM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.342"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..141)
                     /locus_tag="OO322_004356"
     CDS             complement(<1..141)
                     /locus_tag="OO322_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5908941.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT          154 a           70 c           76 g          142 t
ORIGIN      
        1 aaagtctgag aaagcaacgt ttgttttggt tgctttcagc tttacatcat ctgccgcatg
       61 agcagcaaat gcagccatac cagatgccag tatcaaagat atcgaggtac ttacgacact
      121 tttctttaat gcagacttca taaatgttcc tcttcttatt gtacttaatt ccgaagtaat
      181 tttacgaaga gaaataagtg gatgtaagtg aagttagtca cataaagaga tagcagattt
      241 agctaaaaaa agggaaataa cagtccataa agcgttgaca ttactttctg ttctattaag
      301 taatttctcg ccgataaaca actaatttat tgatatttaa taaattattg cattttactg
      361 acaaaatgcc gaattgagat cataaataat catgcaacag gttatgcaag tgcataaata
      421 tgtgatggat gtcacttatt ta
//
LOCUS       DAJTRM010000333          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRM010000333 DAJTRM010000000
VERSION     DAJTRM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.343"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(194..>437)
                     /gene="ydcF"
                     /locus_tag="OO322_004357"
     CDS             complement(194..>437)
                     /gene="ydcF"
                     /locus_tag="OO322_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HCR5908942.1"
                     /translation="GNNADSVIFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRG
                     RDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT          132 a           92 c          101 g          112 t
ORIGIN      
        1 cgactcttgt gatttatatg tttgttttcg gtgaatatat tgttaattaa catcaatcat
       61 cagagagacg gcatcgggaa aggacaagtc tgtggagggc ttcataaaaa caagtgtaaa
      121 aggaataaaa ggcagaaata aaagtcagga gtcatgcccg tttgcaaaca agtggttcac
      181 aaacgggcaa tttttaacgt aacgagcgac tttccatcgc ctcgatgagc accgcatcat
      241 gtttcagcgt ttgccatgca tggatgactt ctgccggaaa atcaacgtga acgataaaat
      301 ctcgcccgcg gggaccgtag ccatcgctat catcgtgtaa acgcggcagc tcgccagtga
      361 gtagtgagag ataacgctca accggccata atccttgtaa ctgattaata aagattacac
      421 tgtctgcgtt atttcct
//
LOCUS       DAJTRM010000334          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRM010000334 DAJTRM010000000
VERSION     DAJTRM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.344"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            19..171
                     /locus_tag="OO322_004358"
     CDS             19..171
                     /locus_tag="OO322_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5908943.1"
                     /translation="MEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTM
                     SQQEVK"
     gene            171..>436
                     /locus_tag="OO322_004359"
     CDS             171..>436
                     /locus_tag="OO322_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5908944.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPV"
BASE COUNT          130 a          103 c          105 g           98 t
ORIGIN      
        1 gaagcttatg aattattcat ggaaaagcgt tttggtgact tagtagatcg tcggagagca
       61 aaaaacggcg ataacgaata catggcatgg gatatgactc tcggttggat cgtctggcag
      121 caacgagctg gtatccattt ctcaacaatg tcacagcaag aggtgaaata atggagccat
      181 acagcctcac actcgatgag gcctgtcatt ttctcaagat atccagaccg actgccatta
      241 actggatacg cacagggcgt cttcaggcaa cacgcaaaga tcccactaag aataaatctc
      301 cttacctcac aacacgacaa gcctgcattg cggctcttca gtctccgctg catactgtcc
      361 aggtgagcgc gggtgatggc ataacagagg aaagaaaatg tcactcttcc gcagaggtga
      421 aatatggtac gccagt
//
LOCUS       DAJTRM010000335          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRM010000335 DAJTRM010000000
VERSION     DAJTRM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.345"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          110 a          117 c           81 g          118 t
ORIGIN      
        1 gaggtgcagg tgcactataa ttccacccgt tctggttttc aatagctaca ttcaacattt
       61 tctctttcct ttcatcaccg aagtgaactt tgttgatgcg gtgcctggtg cctccaggtg
      121 acgttaacca gttaacaatt acagttggct ttcccaccca aaccaataag gactaacatg
      181 acttttaact gtgccgcgtg cgcatagccg cattcaccgc atcacaaaat tcactttaaa
      241 aagggcggac atcagccagc aattaaaccg atgccgccaa ctggtacttc acacagcaat
      301 gtcgttattt acaaccggaa gcgcactccc accatttaaa tttcacagac aagaccgact
      361 ctttatggat accggaaatg cgccttcgtg ttgtgcccgg ttttatttca ccacttccgg
      421 gctttg
//
LOCUS       DAJTRM010000336          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRM010000336 DAJTRM010000000
VERSION     DAJTRM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.346"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          140 a           83 c           60 g          143 t
ORIGIN      
        1 ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt acagggctta
       61 ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca taagaaatca
      121 aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt cattaatact
      181 gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga gatccatcgg
      241 tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg tatcagattc
      301 ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat ttacattatt
      361 tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt aatctcttac
      421 tacttt
//
LOCUS       DAJTRM010000337          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRM010000337 DAJTRM010000000
VERSION     DAJTRM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.347"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..206
                     /locus_tag="OO322_004360"
     CDS             <1..206
                     /locus_tag="OO322_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR5908945.1"
                     /translation="FWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRYALLD
                     YIHETTIRSLDAYPVHSEHQNLN"
     gene            271..>409
                     /locus_tag="OO322_004361"
     CDS             271..>409
                     /locus_tag="OO322_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5908946.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VL"
BASE COUNT          125 a           77 c           79 g          128 t
ORIGIN      
        1 atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta ccaacccagg
       61 tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgggtac cgaaaacgat
      121 acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca tatcctgtac
      181 actcagaaca tcagaattta aattaaggtt tgatgtattc tctgaataaa acagtaatga
      241 taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga gtttattcct
      301 gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct tttgggaatg
      361 attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttgg
//
LOCUS       DAJTRM010000338          407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRM010000338 DAJTRM010000000
VERSION     DAJTRM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.348"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(224..>407)
                     /locus_tag="OO322_004362"
     CDS             complement(224..>407)
                     /locus_tag="OO322_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908947.1"
                     /translation="RDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYL
                     RQRACNGLSDNRCLEI"
BASE COUNT           89 a          102 c          107 g          109 t
ORIGIN      
        1 ggtcgcccgg ccagtgacat tatgcaggca catttgatgg tggtgaataa gtaaaaaagc
       61 ggccatttgg aagccgcctg cggttgattg ccggatgcgg cgtaaacgcc ttatccggcc
      121 tacatgatcg tgcaaattca ataaattgca gcgttctgtg gatactggtt tgggtgatcg
      181 tatccggcaa agcctttacg tgcggtggca gttggatttg cccctatatt tccagacatc
      241 tgttatcact taacccatta caagcccgct gccgcagata ttcccgtggc gagcgataac
      301 ccagcgcact atgcggatgc cattcgttat aatgctcgaa cgcctctgca aggttctttg
      361 ctgccgttaa cccgtctggt ttgggcatga tactgatgta gtcacgc
//
LOCUS       DAJTRM010000339          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRM010000339 DAJTRM010000000
VERSION     DAJTRM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.349"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>406)
                     /locus_tag="OO322_004363"
     CDS             complement(<1..>406)
                     /locus_tag="OO322_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5908948.1"
                     /translation="FKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVNVHG
                     QFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAARKF
                     RHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSA"
BASE COUNT           91 a          106 c          103 g          106 t
ORIGIN      
        1 gcactgtaaa atgtcacgtc atccacgcct ttgaagtatt tcttgatggt tcctactcgc
       61 ttaaggccaa tcattgcgca gtacatctga ccgtggcgga atttgcgcgc agcgaacgat
      121 gtgacgcact gaacggtggt gttagtcaga atgtatcgcc agaacgccag cccgatttcc
      181 ttgctgaatc cacgaacctc tggcaggtac atggcgtggc aatcaaaggt caaaggctga
      241 atctcctgat agtaaacaat gccgccgaac tgcccgtgca cgttcacctc gaagtaacgg
      301 cattcaggct tgtagtcgta tccatcaccg ttgttgctcc cggcgataat gtcggggtga
      361 tttcccacgg cttctatcag gtcgatgttt cgcgttggtt tgaact
//
LOCUS       DAJTRM010000340          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRM010000340 DAJTRM010000000
VERSION     DAJTRM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.350"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(298..>406)
                     /locus_tag="OO322_004364"
     CDS             complement(298..>406)
                     /locus_tag="OO322_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5908949.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          112 a           96 c           81 g          117 t
ORIGIN      
        1 gtccgttaaa ctcaggctac ctcactcccg taaagacagg agtcaagttc ccgctccgac
       61 ataactgcgt gcaatggatt ttgaaggaac cgtcagccca ttccccggag gcaaacatgg
      121 gctggcaaaa gtgtagcggt attaggcaca gctatttagg aaagagggac catctccatc
      181 tcattaaatg gaccacgcca gacaattcac tgtcatgctg atgctgcact gtggggacag
      241 aatcactcat gatgacttcg ataatcgacg acattattat ttggccgagc ttttaggtca
      301 tatctcaaat cgtttgccta atattgctcc attttttaat aaaaaatcaa tcatttttga
      361 atctttgctt ttcattgcat tgtcaagcat agtgtcccca ctattt
//
LOCUS       DAJTRM010000341          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRM010000341 DAJTRM010000000
VERSION     DAJTRM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.351"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..241)
                     /locus_tag="OO322_004365"
     CDS             complement(<1..241)
                     /locus_tag="OO322_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908950.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG"
BASE COUNT          123 a           92 c           71 g          118 t
ORIGIN      
        1 cacctatact tttgccgaat tcataagaag cttttctcaa tataatcgca ccgctggcag
       61 aaggggatgg gcacgatatc ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat
      121 tatctgcaga tgttttaacg ttactaataa atcgtgaaac aatctcctca tttgttagta
      181 ttttcgcctg agcacagcaa gaggttaata ttgcaaccgt cattaaaaat ggctttatca
      241 caaaacaaac tccattgtta gcatgttacg cgataatttt gcctcatctt tcaccacaaa
      301 aaaagaactc accttttaaa tggagaatac ccaacatcat gggtgatttt cataaaggga
      361 tcgcctgttt tcggcaaatc gataacgcgc attccaggcg gcgc
//
LOCUS       DAJTRM010000342          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRM010000342 DAJTRM010000000
VERSION     DAJTRM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.352"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          124 a           95 c           98 g           84 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca g
//
LOCUS       DAJTRM010000343          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRM010000343 DAJTRM010000000
VERSION     DAJTRM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.353"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            243..>397
                     /gene="raiA"
                     /locus_tag="OO322_004366"
     CDS             243..>397
                     /gene="raiA"
                     /locus_tag="OO322_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5908951.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADA"
BASE COUNT          133 a           88 c           71 g          105 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgc
//
LOCUS       DAJTRM010000344          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRM010000344 DAJTRM010000000
VERSION     DAJTRM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.354"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>397)
                     /locus_tag="OO322_004367"
     CDS             complement(<1..>397)
                     /locus_tag="OO322_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5908952.1"
                     /translation="MTTAHNRAQAVFLCVKHSHIQIMVGRAGQPQGWPVSVVTGCSNP
                     VRLTTHEIATSGGESFKLTIEAAIMATILTLSHPDATIENGRAVTTSVAVAEFFRKMH
                     KNVIQKIETLECSPEFNRLNFKPVTYTDAK"
BASE COUNT          100 a           96 c          110 g           91 t
ORIGIN      
        1 ctttggcgtc ggtataggtg acgggcttaa aattaagccg gttaaactcc ggcgagcatt
       61 cgagagtttc gatcttctga ataacgtttt tgtgcatttt ccggaagaac tccgcaaccg
      121 caacagacgt agtgacagcg cgaccatttt cgatggttgc gtcagggtga gaaagggtaa
      181 ggatagtagc catgatggca gcctcgatag tcagtttaaa tgactcacca ccagaggtag
      241 caatctcatg ggtggtgaga cgcacagggt tgctacaacc ggtgactacc gaaaccggcc
      301 agccttgcgg ctgccctgca cgccccacca taatctgaat gtggctgtgc ttgacgcata
      361 aaaaaaccgc ctgagcgcgg ttatgcgcag tagtcat
//
LOCUS       DAJTRM010000345          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRM010000345 DAJTRM010000000
VERSION     DAJTRM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.355"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(84..>396)
                     /locus_tag="OO322_004368"
     CDS             complement(84..>396)
                     /locus_tag="OO322_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5908953.1"
                     /translation="YSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTL
                     IHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKR
                     D"
BASE COUNT          121 a          105 c           96 g           74 t
ORIGIN      
        1 cagcgatttt gacgccacca atacccgtgc ggtttgtaat gtcgaagata aaacaaccag
       61 ggcagataaa atgccaatgc taattaatca cgcttttcca gcagagtccg gtaaatcaga
      121 ccaccgataa tgccgccgac aattggcacc acccagaaga accacagttg ttctaatgcc
      181 cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg ggttaacaga agtgttagtc
      241 accggaatac tgattaagtg aatcagggtt aaggccagac caatagcgat cggcgcaaaa
      301 cctgccggag cgaatttgtc ggttgcaccg tggatcacta acaggaagcc cgcactcaat
      361 accagttcaa ctaccagcgc ggaaagcatg gaataa
//
LOCUS       DAJTRM010000346          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRM010000346 DAJTRM010000000
VERSION     DAJTRM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.356"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..264)
                     /locus_tag="OO322_004369"
     CDS             complement(<1..264)
                     /locus_tag="OO322_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908954.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLP"
     gene            complement(261..>394)
                     /locus_tag="OO322_004370"
     CDS             complement(261..>394)
                     /locus_tag="OO322_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908955.1"
                     /translation="VPDTLPVVRKASWHCRQCHHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT           88 a          119 c           96 g           91 t
ORIGIN      
        1 cggtaatggc gataaaaaca tcgccatatc ctcaatgtaa gggtgggact tctccggatt
       61 cagcaccaca cagtccgcct tctgttgctc actcagggca tgtaaatcat gctcaaacca
      121 cgccccctga gcatctgtct gcagaatcaa ccggccatgg caggaaaggc agaaacactg
      181 ccggatattt ctgctgagcg cagagtcggc actgataatg tgctcacccg gcgtgatccc
      241 cagccccgtt tttataccgt tcattctgct cccccctcct cactgaagcg cccggtctgg
      301 cagtgtgtgc agtactgctc cccataataa tcgtggtgac attgcctgca gtgccagctg
      361 gctttacgta ctacgggtaa ggtatccggg acga
//
LOCUS       DAJTRM010000347          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRM010000347 DAJTRM010000000
VERSION     DAJTRM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.358"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..151
                     /locus_tag="OO322_004371"
     CDS             <1..151
                     /locus_tag="OO322_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5908956.1"
                     /translation="PEIFAEAAHTLAFETLEHATRADHIDSDRYLIKSTYYNLFSGEY
                     SNKKT"
BASE COUNT          121 a           79 c           85 g          102 t
ORIGIN      
        1 gcctgagata tttgctgaag ccgcacatac cctggcattt gaaacattag agcatgcaac
       61 tcgtgctgac catatagatt ctgacagata tctaataaaa tcaacttatt ataatttatt
      121 tagtggcgaa tattctaata aaaaaacata gctgcagagc aagcaattcg gttgttggca
      181 agtcaagggc aaagcattcg cgtgagaatg ggcgtatgct ttgtgccaaa agcaaacgtt
      241 gctaattctg cactgttcag cgatggcgca gtagagacag gcactgccac tgtccagtcg
      301 gcaaaagcga acactttgcc actgcgggga taaactgatt gaagctctcg cgcaaaatca
      361 cttattcttt tgcgttaata aaatgta
//
LOCUS       DAJTRM010000348          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRM010000348 DAJTRM010000000
VERSION     DAJTRM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.359"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>385
                     /locus_tag="OO322_004372"
     CDS             <1..>385
                     /locus_tag="OO322_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5908957.1"
                     /translation="NRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDLILNPS"
BASE COUNT          113 a           88 c          102 g           82 t
ORIGIN      
        1 aaaccgcgca attggcgttg cgctgaatga tactgcatgt cgcgtattga aaaaacaaat
       61 cggaaatcat caccgttggg tatttgtgta caaggaaagc tgtaccaaac cagacggaac
      121 gaaagcgcca acagtaagga agatgcggta tgacgcaaac acagcctgga aagcggcgct
      181 gagacgggct ggtattgatg atttcagatt tcacgacttg agacacacct gggcaagttg
      241 gctggttcaa gccggagtcc cgttatcagt gttacaggaa atgggaggct gggagtctat
      301 cgaaatggtt cgtcgatatg ctcacctcgc acctaatcac cttaccgaac acgcacggca
      361 aatagacttg attctgaacc catcg
//
LOCUS       DAJTRM010000349          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRM010000349 DAJTRM010000000
VERSION     DAJTRM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.360"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          110 a           94 c           67 g          114 t
ORIGIN      
        1 tttaaaacca atgcacatgg tctcactgtc taaaattatt gcttctgacc taactgcctc
       61 tgacgaccac gcatacgttt gaggctgaat cctgatgcca aagctttcat cagcagaata
      121 atttcctctg ctgaaatggc aggagctgac actaacactt acgcactggc ctaaaaagtg
      181 aagaccgact ctacaggttc cagcctgaca tgctataaat tttgagtgcc agagcccttg
      241 cattaaataa tgcggtttca acttcattca ccattacatt atcaaatacc cagccataaa
      301 cctgtctgat gacagagtct tctgctgtaa tgtttgtgtc aataccattg caggtgttat
      361 taccagttac tcgtatattt ctcag
//
LOCUS       DAJTRM010000350          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRM010000350 DAJTRM010000000
VERSION     DAJTRM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.361"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>375
                     /locus_tag="OO322_004373"
     CDS             <1..>375
                     /locus_tag="OO322_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908958.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLDAAARLQYEQARDK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELAL"
BASE COUNT          118 a           72 c           87 g           98 t
ORIGIN      
        1 aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct gattgcgctg
       61 gaaagtcgtc tgcatcaaaa gcataaactg gatgctgctg cacgccttca gtatgagcaa
      121 gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct ggttgatgag
      181 cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga taatcaaggc
      241 gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt agtcgtcaat
      301 gagttgcaga ttgaaatgct ggcgcgagca atcattcatg caattaataa tgcggaaatg
      361 cgtgaactgg catta
//
LOCUS       DAJTRM010000351          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRM010000351 DAJTRM010000000
VERSION     DAJTRM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.362"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(213..>374)
                     /locus_tag="OO322_004374"
     CDS             complement(213..>374)
                     /locus_tag="OO322_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908959.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          125 a           91 c           63 g           95 t
ORIGIN      
        1 tcactccctg cttctggcaa atatgagctg cgagtgctgc aaactcgaaa cgacgcccgt
       61 aaaaacaaaa ctaaaaaata taacgtcgat attcaaatta agtaaatgtc aaccgacggg
      121 gactatcccc gtcatagacg ttaacgcccc actcctctct gtcgctcaag aggttaatga
      181 cgaatagcag agaacataat gtaaacaagg catcactcac gacacataaa taacatcttt
      241 gtggtggttt gattgatttt tccactccgc ttaccatcta caccaaaata acgatcatca
      301 cgacataatt tttctttaac atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga
      361 tcgtagcctt gtaa
//
LOCUS       DAJTRM010000352          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRM010000352 DAJTRM010000000
VERSION     DAJTRM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.363"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            70..>374
                     /locus_tag="OO322_004375"
     CDS             70..>374
                     /locus_tag="OO322_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908960.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE"
BASE COUNT          119 a           79 c           98 g           78 t
ORIGIN      
        1 tgccggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat tatgcttcca
       61 tcggaaacga tgatctgtca gcctgagttc acagataaaa cactctccag gaaacccggg
      121 gcggttcaga aagtaaatga aacagatgct gaatacctgg tgcgtaaatt cgatgagctt
      181 gaagctaaat gtgcagcaca ggaaaacaaa gtaataccag tgtcaactga actgccacca
      241 gcaaatgaaa gtgttttgtt attcgatgct aacggagaag gctggctaat tggctggcgt
      301 tctctctggt acacctgggg acaaaaagaa accggagaat ggcagtggac atttcaggtc
      361 ggggaccttg aaaa
//
LOCUS       DAJTRM010000353          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRM010000353 DAJTRM010000000
VERSION     DAJTRM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.364"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..311)
                     /locus_tag="OO322_004376"
     CDS             complement(<1..311)
                     /locus_tag="OO322_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5908961.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTRM010000354          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRM010000354 DAJTRM010000000
VERSION     DAJTRM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.365"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..145
                     /locus_tag="OO322_004377"
     CDS             <1..145
                     /locus_tag="OO322_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908962.1"
                     /translation="QQLEPNAGTGARIWLAKEALESGDYDSEEAFYKAEGRAGYSPGL
                     GGV"
BASE COUNT          102 a           87 c           95 g           86 t
ORIGIN      
        1 ccagcaactg gagccaaatg caggcactgg ggcacgcatc tggctggcta aagaagcgct
       61 ggaatctggc gattatgata gcgaagaagc cttctacaaa gcagaaggcc gtgcaggata
      121 ttcaccgggt cttggcgggg tataaatacc atgcgcattg actgaattcg caaacaaaaa
      181 cagacgcgcg atacccggat agtcggtctg catagtcaga tatctggctg cgaatttgat
      241 aaatatcacc gcccttttcg gaaacataag ttccgtccgg gagttgatta tacgaaccat
      301 ctccatgggg gatcgttctc ctgaatccta gtgagagcat atatttatga agcccttcgt
      361 aatcctctgg
//
LOCUS       DAJTRM010000355          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTRM010000355 DAJTRM010000000
VERSION     DAJTRM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.366"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..106)
                     /gene="hokE"
                     /locus_tag="OO322_004378"
     CDS             complement(<1..106)
                     /gene="hokE"
                     /locus_tag="OO322_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5908963.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKE"
BASE COUNT           97 a           99 c           76 g           94 t
ORIGIN      
        1 gttcctttac ggtgaactca cacagcgagt ctccgaccag aagcgtaaat cccagcaccg
       61 tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt atattgcctc
      121 catgcgaaga agggactacc atccgagttg ttgaggttcg gagtggcagc cccaggattg
      181 atagaaatat ctcttggggc ttcatctttc caaacctcag agttaacctg aaaccggaaa
      241 gcttcaggca cccgccgtta tcttaccctt ccttagcaaa atagctgttt atttatacag
      301 tgagtcctgt ttcggacttc tgtcggcaaa actcatcatt gctcttaaaa cgaaatcgcc
      361 cgcagc
//
LOCUS       DAJTRM010000356          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRM010000356 DAJTRM010000000
VERSION     DAJTRM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.367"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            143..>363
                     /locus_tag="OO322_004379"
     CDS             143..>363
                     /locus_tag="OO322_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5908964.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNA"
BASE COUNT          126 a           80 c           46 g          111 t
ORIGIN      
        1 gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt cagaaaacaa
       61 acttattttt attaaaatca attctttttc tttgaacatt caactgcgat atttgtaaaa
      121 acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat tccccgcata
      181 tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc aatacgcacg
      241 cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac gtactgttga
      301 gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa cgaagtgtaa
      361 tgc
//
LOCUS       DAJTRM010000357          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRM010000357 DAJTRM010000000
VERSION     DAJTRM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.368"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT          116 a           54 c           74 g          107 t
ORIGIN      
        1 gggtgaagtg ctgattgaaa gaatagataa gaaagcgtaa ccgcgggggc agaatgggga
       61 ttaagtagcc agatatgtgt tactaaatgt aactaacaag ctaaccttca aacgggggtt
      121 aatctttgaa cgtatgtcat gtttcaaccc ttcagatcgt gaatctaaag ggttacatat
      181 taactatatt ctcaacaaga ccagacactt atagaattag tgatgattta ttcactaaaa
      241 aggccatgaa ttggaggaaa acgatctgtc cagaaagtct atcggttcct ctcttattgc
      301 ctgattgtaa taatcgttat tatagtaagt attgtttatt tcatctaagg t
//
LOCUS       DAJTRM010000358          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRM010000358 DAJTRM010000000
VERSION     DAJTRM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.369"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO322_004380"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO322_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5908965.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTRM010000359          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRM010000359 DAJTRM010000000
VERSION     DAJTRM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.370"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..228
                     /locus_tag="OO322_004381"
     CDS             <1..228
                     /locus_tag="OO322_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5908966.1"
                     /translation="ERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPE
                     AYLRHILSVLPEWPSNRVDDLLPWKVVLPSG"
BASE COUNT           80 a           87 c           98 g           78 t
ORIGIN      
        1 gaaagagcgc ttcgtgcagt ctgtctcgga aagaaaaatt acgtgttctt cggtagcgat
       61 cacggcggcg agcgtggtgc actgctgtac gggctgatcg gcacctgccg tctgaacggt
      121 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccttccaac
      181 cgagttgacg acctcctgcc atggaaggtg gtgctaccat caggataatc gtcaatacgg
      241 tgttcggtga acgtttactt ggagcatcat gcttcatgaa gaaaaactgg cacgtcatca
      301 acgtaaacag gcgatgtata cccgaatggc agccttcccg gcg
//
LOCUS       DAJTRM010000360          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRM010000360 DAJTRM010000000
VERSION     DAJTRM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.371"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>340
                     /locus_tag="OO322_004382"
     CDS             <1..>340
                     /locus_tag="OO322_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5908967.1"
                     /translation="YLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGV
                     MFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYV
                     SFSEAAHAIT"
BASE COUNT           82 a           85 c          108 g           65 t
ORIGIN      
        1 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
       61 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      121 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      181 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      241 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      301 gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAJTRM010000361          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRM010000361 DAJTRM010000000
VERSION     DAJTRM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.372"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..>332
                     /locus_tag="OO322_004383"
     CDS             <1..>332
                     /locus_tag="OO322_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5908968.1"
                     /translation="WARRPSGTIHHSDKGSQYVSLAYKARLKEAKLLASTGSTGDSYD
                     NAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGND"
BASE COUNT          100 a           78 c           92 g           62 t
ORIGIN      
        1 tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca gtatgtgtca
       61 ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg gagtactggt
      121 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
      181 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      241 tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc agaagcagaa
      301 aaagcttatt atgcttccat cggaaatgat ga
//
LOCUS       DAJTRM010000362          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRM010000362 DAJTRM010000000
VERSION     DAJTRM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.373"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>331)
                     /locus_tag="OO322_004384"
     CDS             complement(<1..>331)
                     /locus_tag="OO322_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5908969.1"
                     /translation="GINPRLMSVNRNSYSNDLLIRSGQNIYLNGVHLTANADSFEIIR
                     WIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWRKQLTPDRQWSKW
                     IDLPGIEP"
BASE COUNT           94 a           78 c           72 g           87 t
ORIGIN      
        1 aggttcaata cctggtaggt ctatccactt gctccactga cggtcgggcg tgagctgttt
       61 acgccagcga acgcgacttt cccctacttc aaaagagacg tcatcatcta cgggtatcgc
      121 tttgcctgat aattgaccaa agggataacg atgcagaccc ttattgtcgc gaaaaaccag
      181 cagtgaatga ggtatccagc gaattatctc aaatgagtct gcattcgccg taaggtgaac
      241 gccatttaaa taaatattct gcccactgcg gataagcaga tcattggaat atgaatttct
      301 gtttacactc ataagacgtg gattaatacc c
//
LOCUS       DAJTRM010000363          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRM010000363 DAJTRM010000000
VERSION     DAJTRM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.375"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..230)
                     /locus_tag="OO322_004385"
     CDS             complement(<1..230)
                     /locus_tag="OO322_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5908970.1"
                     /translation="MERIMQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIP
                     EVCNLLFTPTIYLLLNIFMLTLGALMLFFSGRV"
BASE COUNT          100 a           79 c           66 g           78 t
ORIGIN      
        1 actcgccccg agaaaaatag cattaatgcg cccagcgtca gcataaaaat attgagcaga
       61 aggtaaattg ttggcgtaaa gagtaaattg caaacctcag gaatagcggc aagcccagcc
      121 cccagcatgg caataaccgc tgcaaaaaat tgcgtcagta ttgggatgcc gaaaaaagtc
      181 attaccagcg aggtcaaaat ccattttcta ttttgcatta ttctttccat tctttctgaa
      241 tggcgaaatt atactccccg agtccccttg ccccttctgg acacttttcc gaaatgatgg
      301 cgaaaaaaac gggacccttt ggc
//
LOCUS       DAJTRM010000364          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRM010000364 DAJTRM010000000
VERSION     DAJTRM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.376"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
BASE COUNT           99 a           64 c           61 g           87 t
ORIGIN      
        1 actggtctgt accctggaga ctttccggtt ttatctggaa aaacagggat ggactcttgt
       61 taaccggggc gcaattaaaa atatgactgt ggaacaatta ctttctttaa aataaaaatg
      121 gagctacgga tatgaaccct atgcttgatt attgttttac ctctgcacct cagcagacca
      181 taacacacac cggaatcaaa aatttgttat ggataatcaa aaaaaacctc cagtagaaag
      241 gtgatcccga tttaattccc tgtgcttatg gcctgtgtga cagacaagta agccctgata
      301 aggcttacac t
//
LOCUS       DAJTRM010000365          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRM010000365 DAJTRM010000000
VERSION     DAJTRM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.377"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..184
                     /locus_tag="OO322_004386"
     CDS             <1..184
                     /locus_tag="OO322_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5908971.1"
                     /translation="VRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLA
                     EQVLMHEPLARMRWCG"
BASE COUNT           76 a           66 c          105 g           64 t
ORIGIN      
        1 agtgagaaaa acggcgatgc aaatcacctg gagagggatg tatcagaggt tactgaaggt
       61 tgacccgctg aagtgcgtgc tgtgcgggag tcagatgcgt tttacggggc tgaagcgggg
      121 ctaccgtctg gcagagcagg tcctgatgca tgagccgctg gcccggatgc ggtggtgcgg
      181 ctgagagccg cagaggggaa gttgcgtcca ttttgaggga aacggagcaa aaaaccgcca
      241 ttcataccct gaatcaatca gagccatcca gtttaatcgt cggttccgtt catatggagc
      301 aaaagtggtg t
//
LOCUS       DAJTRM010000366          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRM010000366 DAJTRM010000000
VERSION     DAJTRM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.379"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..>309)
                     /locus_tag="OO322_004387"
     CDS             complement(<1..>309)
                     /locus_tag="OO322_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908972.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     "
BASE COUNT           73 a           75 c           82 g           79 t
ORIGIN      
        1 ttaaccggct cataaaaata gacatagcgg tcattgagat cgagataata gtgaaatttc
       61 tcttcggcca tcccaagacc tgaaccgtaa tcatcctggt ggcggttgcc ttccactgcc
      121 tgatcaaaag cgggtaatag tgctgcagct tccacaattg tgtcgcagtc agtggatgcg
      181 gtttgtagtg tgccgcgtaa gcccgggtat ttatgggcag tcaggtttaa gccatgggta
      241 ttattggcag actcaaaagc ctcgcagatg gcgcgccgct gttctggcga cacttccgac
      301 tgctcagca
//
LOCUS       DAJTRM010000367          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTRM010000367 DAJTRM010000000
VERSION     DAJTRM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.383"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(<1..142)
                     /locus_tag="OO322_004388"
     CDS             complement(<1..142)
                     /locus_tag="OO322_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5908973.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
     gene            complement(154..>301)
                     /locus_tag="OO322_004389"
     CDS             complement(154..>301)
                     /locus_tag="OO322_004389"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="NAD(P)H-dependent; catalyzes the reduction of
                     2,3-diketo-L-gulonate to 3-keto-L-gulonate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate reductase"
                     /protein_id="HCR5908974.1"
                     /translation="MDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAK
                     IQAL"
BASE COUNT           66 a           76 c           87 g           72 t
ORIGIN      
        1 gcgcataaat attcttgccg tcgatttcga caacgcccgg ctccaggacg ttgaagttgg
       61 tagcgcgcag aaaatcgagc gccttttcaa tggcggcggg caaacggcac ggattgggct
      121 gcgcgatatg tccaaaaatc atgacttatc tcctcataac gcctggattt tggcccacac
      181 gctgtcatca acagtgatgc cgttacggcg gttttcggcc agcagggtag taaattcgtg
      241 gccgggtaag cggatggcct ggttttcgtc agcgcgttca gcggtagtaa cgtaatccat
      301 g
//
LOCUS       DAJTRM010000368          299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTRM010000368 DAJTRM010000000
VERSION     DAJTRM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.denovo.384"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(56..250)
                     /locus_tag="OO322_004390"
     CDS             complement(56..250)
                     /locus_tag="OO322_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5908975.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT           77 a           81 c           56 g           85 t
ORIGIN      
        1 catcgtagcc tttaaaaacg aattttaaag gcaccctaaa ggcaggtcaa tgaagttaac
       61 ccgccttcgc gtttacactc tctgagagaa tactagcttc aatctcttcc aaaatctctc
      121 tgacttgctc gtcgggcata tcaacgagca tcaacgctgc caggcgttta cggccctcta
      181 gttgcagtag ctcttgagtt tccttatcta acaagcgtag cacagtctca acacctttcc
      241 ttgcgagcat gtttgtaagg gcttctcgtt cctcactggt aagaaacgac aacatttct
//
LOCUS       DAJTRM010000369         5962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.169, whole genome shotgun sequence.
ACCESSION   DAJTRM010000369 DAJTRM010000000
VERSION     DAJTRM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.169"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO322_004391"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO322_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5908976.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO322_004392"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO322_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5908977.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3232
                     /locus_tag="OO322_004393"
                     /pseudo
     CDS             2399..3232
                     /locus_tag="OO322_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(3342..3746)
                     /locus_tag="OO322_004394"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO322_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO322_004395"
     CDS             complement(3790..4179)
                     /locus_tag="OO322_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908978.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO322_004396"
     CDS             4397..4837
                     /locus_tag="OO322_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5908979.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5582..5731)
                     /locus_tag="OO322_004397"
     CDS             complement(5582..5731)
                     /locus_tag="OO322_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908980.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1452 a         1570 c         1551 g         1389 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actatatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     5761 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     5821 cttactatgt tgcaacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     5881 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     5941 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJTRM010000370         5961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.170, whole genome shotgun sequence.
ACCESSION   DAJTRM010000370 DAJTRM010000000
VERSION     DAJTRM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.170"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO322_004398"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO322_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5908981.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1640..2398
                     /locus_tag="OO322_004399"
     CDS             1640..2398
                     /locus_tag="OO322_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5908982.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2398..3231
                     /locus_tag="OO322_004400"
                     /pseudo
     CDS             2398..3231
                     /locus_tag="OO322_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(3341..3745)
                     /locus_tag="OO322_004401"
                     /pseudo
     CDS             complement(3341..3745)
                     /locus_tag="OO322_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3789..4178)
                     /locus_tag="OO322_004402"
     CDS             complement(3789..4178)
                     /locus_tag="OO322_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908983.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4396..4836
                     /locus_tag="OO322_004403"
     CDS             4396..4836
                     /locus_tag="OO322_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5908984.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5581..5730)
                     /locus_tag="OO322_004404"
     CDS             complement(5581..5730)
                     /locus_tag="OO322_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5908985.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1452 a         1570 c         1551 g         1388 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat atttttttgg
     1621 tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg
     1681 acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc
     1741 tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat
     1801 caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc
     1861 ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact
     1921 tggcgctgtt cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat
     1981 gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga
     2041 cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt
     2101 gcggctcgac caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa
     2161 cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg
     2221 aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc
     2281 agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc
     2341 ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg
     2401 ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac
     2461 accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag
     2521 ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg
     2581 cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa
     2641 tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc
     2701 gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct
     2761 gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa
     2821 aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc
     2881 aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc
     2941 agtcgcggct ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc
     3001 gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt
     3061 gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac
     3121 caggcgtacg cttatgggtg cctttccgca gcttggaacg cggatggaga agaggagcaa
     3181 cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata ctatatatca
     3241 agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc gctggccaaa
     3301 gccttagcgt aggattccgc cccttcccgc aaacgacccc aaacaggaaa cgcagctgaa
     3361 acgggaagct caacacccac tgacgcatgg gttgttcagg cagtacttca tcaaccagca
     3421 aggcggcact ttcggccatc cgccgcgccc cacagctcgg gcagaaaccg cgacgcttac
     3481 agctgaaagc gaccaggtgc tcggcgtggc aagactcgca gcgaacccgt agaaagccat
     3541 gctccagccg cccgcattgg agaaattctt caaattcccg ttgcacatag cccggcaatt
     3601 cctttccctg ctctgccata agcgcagcga atgccgggta atactcgtca acgatctgat
     3661 agagaagggt ttgctcgggt cggtggctct ggtaacgacc agtatcccga tcccggctgg
     3721 ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc aatactgtgt ttacatacag
     3781 tctatcgctt agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt tgctagacat
     3841 tgccagccag tgcccgtcac tcccactctt acattgttcg cagtatctag gtacatgtta
     3901 ttgcgtgcac cttcatgatg tataatgtta ttaatgacag cagaaagatc gacagaatta
     3961 atattcttcg tacctgggac agttatcggg tgtcggccag tgatgagacc aaaggatgca
     4021 gtataagatg aaccactact attatcatgg ccaccattac caccaccgtt aaaactacct
     4081 cccgaacttc cagagccgct atgaccatca ttgtaggggt catttggtcc catgtgacca
     4141 aaaccaccga cagtaccgct atttaactct gtactcataa aattatcctc tttgactttt
     4201 aaaaacagca aaaactgtat ttataacaat gtgtttatat acagtattcg aaatactacg
     4261 cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt ttcacgcctc acgggcagcc
     4321 tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt acaatacggt acacacagga
     4381 gacactgtca cttttatggc atggcacaca gttcaagaga cactgaaatt aacaggaaag
     4441 tcgcgctcgc aattatatcg tgacatggct aaaggccttg tcagctatcg cacaggaaaa
     4501 aatgatcgcc gggagttcga gaccagtgaa ctgattcggg cctatggcga gctgcgcact
     4561 tctgagacac caaaaaggca caaggaggga cactccaaat ctgtcgttag ccaggcggaa
     4621 aatgctcagc tagacgtcat tcagcagaaa ctggacgctc tgcagcagac tgttacccta
     4681 atgcttgaag acaagatggc gcgggaagca gaaaaccgcc tgcgaaatga ggagagggaa
     4741 caactacagg ccgaaattag ccgtctggct gacgcgctgg cacaggaaaa aaagcgggga
     4801 ttctggtcgc ggctgtttcg ctccggttca gagtaacagt gcaggtgact ctgatttacc
     4861 ataacatctg ttatcagcac cgcctgagag gccctcaggg cgtttctgac gggaaaccgg
     4921 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     4981 catgcatgag tatgcgcaca agtgcatagc gcggaaattc acaccattca gccggggtaa
     5041 gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt
     5101 ggccgtgaag cgttgccggc gcagccggaa aacccgctgt cagcacgttc aggaaggggg
     5161 tgatgtacaa atggcacaga ggatgaaagt gacgtatgag ctgaaggaat gatgtaaaag
     5221 tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc
     5281 aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct ttttttgtca
     5341 cgatctcccc ttaacctgcg taaatcaccc gccatggttg attatttttc aacctcaaaa
     5401 tggtcataca cctaacgcag ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa
     5461 aaggaatatt tcgttttcac gagtaatacc cgggtattaa aaatccactg taattacatg
     5521 aaaataaaga aataaaaggt aataccggag tattaccacg tacgcgggca agcgaaaaag
     5581 ctataacgct tcccggccga caggcgaccc aaaatccaca ggggcatgca ggttctcttc
     5641 agtaacctgc gccattaaat caaaaaggtc atattcgccg ggaagctgcg tatcagtcga
     5701 atccagttgt tcatcatatt cagatggcat ggggtgatat ccagtagctg agaggttgtt
     5761 agcgggtttc ggggcgcagc cctgaaccag tcatgtagcg ctagcggagt gtatactggc
     5821 ttactatgtt gcaacgattg cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag
     5881 gcagcaccgg cgcgtcagca gaatatgcga tacaggagat attccgcttc ctcgctcact
     5941 gactcgctac gctcggtcgt t
//
LOCUS       DAJTRM010000371         5832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.174, whole genome shotgun sequence.
ACCESSION   DAJTRM010000371 DAJTRM010000000
VERSION     DAJTRM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.174"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            <1..255
                     /locus_tag="OO322_004405"
     CDS             <1..255
                     /locus_tag="OO322_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR5908986.1"
                     /translation="GDYSRLVQPVRSEETLHSLAHGAGRKWGRTECKGRLAAKYTATQ
                     LSRTELGSRVICRDKQLIFEEAPQAYKSAESVVQCQGRPD"
     gene            317..931
                     /locus_tag="OO322_004406"
                     /pseudo
     CDS             317..931
                     /locus_tag="OO322_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(988..2445)
                     /gene="pepD"
                     /locus_tag="OO322_004407"
     CDS             complement(988..2445)
                     /gene="pepD"
                     /locus_tag="OO322_004407"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5908987.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2707..3165
                     /gene="gpt"
                     /locus_tag="OO322_004408"
     CDS             2707..3165
                     /gene="gpt"
                     /locus_tag="OO322_004408"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5908988.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3257..4501
                     /gene="frsA"
                     /locus_tag="OO322_004409"
     CDS             3257..4501
                     /gene="frsA"
                     /locus_tag="OO322_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5908989.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4559..4960
                     /gene="crl"
                     /locus_tag="OO322_004410"
     CDS             4559..4960
                     /gene="crl"
                     /locus_tag="OO322_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5908990.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(4999..>5832)
                     /gene="phoE"
                     /locus_tag="OO322_004411"
     CDS             complement(4999..>5832)
                     /gene="phoE"
                     /locus_tag="OO322_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5908991.1"
                     /translation="GRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGA
                     LYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKN
                     ENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATG
                     LKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGK
                     DIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQ
                     F"
BASE COUNT         1413 a         1452 c         1526 g         1441 t
ORIGIN      
        1 ggtgattact ccaggctggt tcagcccgtc aggagtgagg aaacattgca ttcgctggcg
       61 catggggctg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac
      121 acagcgacgc aactctcgcg tactgaactt ggcagtcggg taatttgtcg cgataaacaa
      181 ctcatcttcg aagaagcgcc acaagcctat aaatcggctg aaagtgtggt gcaatgtcag
      241 ggcaggccgg attaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca
      301 gtggagggaa aaaaggatga tcttgctata actctcctca gcgcaggggc cggaagaatg
      361 ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt
      421 cgcggtaacg gtgctggaaa cagaaacagg ccgctactct gacacgctgc gttcggcgct
      481 gatttctctg gatggcgaca acgcatgggc gttaagcgaa agctggtgtg gcactattca
      541 gtggatttgc ccgagcccgt atcgcccaca tcacgggcgt aaaaactggt ttctgggcat
      601 tgggcgtttt accgctgatg agcaggagca atccgatgcg atccggtatg agacgctgcg
      661 ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca
      721 tttggcatcc ggtattagcg tgaaggttca gtcagagcgc agccagcatg ctaacaaacg
      781 gctggcgcga ttgctgattg cctggaagct ggatcagcag caataggaaa atagcgcggt
      841 gctgaaatca cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgcgacgaac
      901 gtttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta
      961 aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt
     1021 cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg
     1081 accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag
     1141 accacattcc aggcccgcgt ggataatctg gatgttcggc gtcttgttga acaggcgctg
     1201 ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata
     1261 tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac
     1321 cacgtagtct ttaccgctgt cgattagtga acggatcagg cagtgaattt ctacgttatt
     1381 gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc
     1441 ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga
     1501 tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt
     1561 ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag
     1621 tgcgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt
     1681 acgcagtgtg ccgccgttga aatcgataag gcgcaggtcc agttcttctg catgacccgc
     1741 caggaagcgc accagcagtt tgttggcatt acccaggcca acgtggattt caccgccgga
     1801 gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc
     1861 ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat
     1921 ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg
     1981 taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg
     2041 gccgtgaacc acgttttcgt cagccagaac cgccagtgca gaggccatac caatgccgtt
     2101 atccgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat
     2161 aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg
     2221 ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt gacgaatcag
     2281 gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat
     2341 gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa
     2401 aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt
     2461 cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa
     2521 gacaggcttg cgagatatgt attgagaata ccactttatc ccgcgtcagg gagaggcagt
     2581 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     2641 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     2701 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     2761 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     2821 ggtctggtac cgggggcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     2881 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     2941 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgctgtt
     3001 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     3061 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat cgaacagccg
     3121 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     3181 gcattgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc cagtaagtat
     3241 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     3301 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     3361 gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     3421 gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac gtattgtgat
     3481 gagcgatgct gaacgtaccg acgatgattt atacgatacc gtgattggct accgtggtgg
     3541 caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtacgga
     3601 agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt acaacattgc
     3661 cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg
     3721 cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
     3781 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     3841 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     3901 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
     3961 ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc
     4021 gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     4081 cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag cggttgcctg
     4141 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
     4201 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt
     4261 acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc
     4321 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     4381 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
     4441 tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta
     4501 aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat
     4561 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
     4621 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     4681 cgtcaacgtg aaaccggcac cggaagtgcg tgaattctgg ggctggtgga tggagctgga
     4741 agcgcaggaa tcccgtttta cctacagcta ccagtttggt ctgttcgata aagcaggcga
     4801 ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg
     4861 tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc
     4921 gaatgatttt cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat
     4981 cacatccggc aatattcatt aaaactgata cgtcatccca accgcgacag tatcatcatt
     5041 attaatattc agcttattat cgctatccag ttggttgatt ttataatcaa caaacgctga
     5101 catatttttg ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc
     5161 accgatgccc tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc
     5221 aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc
     5281 agttattggc gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc
     5341 atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag
     5401 gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc
     5461 gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg
     5521 gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa
     5581 gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg
     5641 cgccgaggaa tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa
     5701 cgcgcccagg ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa
     5761 agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg caaactccgc
     5821 ttcccaccga cc
//
LOCUS       DAJTRM010000372         4187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.196, whole genome shotgun sequence.
ACCESSION   DAJTRM010000372 DAJTRM010000000
VERSION     DAJTRM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.196"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            118..1250
                     /locus_tag="OO322_004412"
                     /pseudo
     CDS             118..1250
                     /locus_tag="OO322_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1339..1872
                     /gene="blc"
                     /locus_tag="OO322_004413"
     CDS             1339..1872
                     /gene="blc"
                     /locus_tag="OO322_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5908992.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1869..2186)
                     /gene="sugE"
                     /locus_tag="OO322_004414"
     CDS             complement(1869..2186)
                     /gene="sugE"
                     /locus_tag="OO322_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5908993.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2362..2508)
                     /gene="ecnB"
                     /locus_tag="OO322_004415"
     CDS             complement(2362..2508)
                     /gene="ecnB"
                     /locus_tag="OO322_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5908994.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2619..2744)
                     /gene="ecnA"
                     /locus_tag="OO322_004416"
     CDS             complement(2619..2744)
                     /gene="ecnA"
                     /locus_tag="OO322_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5908995.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2796..3362)
                     /gene="efp"
                     /locus_tag="OO322_004417"
     CDS             complement(2796..3362)
                     /gene="efp"
                     /locus_tag="OO322_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5908996.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3404..>4187
                     /gene="epmB"
                     /locus_tag="OO322_004418"
     CDS             3404..>4187
                     /gene="epmB"
                     /locus_tag="OO322_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5908997.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVN"
BASE COUNT         1057 a         1086 c         1043 g         1001 t
ORIGIN      
        1 cggtctggct gctatcctga cagttgtcac gctgattggt gtcgttacaa tctaacgcat
       61 cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg
      121 ttcaaaacga cgctctgcgc cttattaatt accgcctctt actccacatt tgctgcccct
      181 caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc
      241 cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat
      301 gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc
      361 agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta
      421 agcgatcccg caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca
      481 ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg
      541 aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg ggctccagga
      601 acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg
      661 tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact caaactcaac
      721 catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa
      781 ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg
      841 accattgaag atatggcccg ctgggtgcaa agcaatttac cccccttgat atcaatgaga
      901 aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
      961 atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg
     1021 gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc
     1081 ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg
     1141 tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca
     1201 atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     1261 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     1321 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     1381 ctggtcgttg cctgtagttt tcctacgccg ccgcgtggcg tgaccgtagt gaataatttc
     1441 gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     1501 cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc
     1561 attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac
     1621 tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     1681 ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac
     1741 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     1801 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     1861 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     1921 ctcgccagac gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg
     1981 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctaccggtaa cgatttcatc
     2041 gcccaggcaa gtagcgccat actgactatc atcgccgtca cggtaataac actcggcgtc
     2101 aaacggctaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     2161 gcaataacta agataatcca ggacatatca ggctccggaa atttggggcc gtccccggtg
     2221 aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
     2281 ggcaggggaa tttcaactat tacctttatg acgatcgcag aaaaaacaaa cggcacgaca
     2341 ctgctgtcgt accgttattg cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt
     2401 accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt
     2461 tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt
     2521 ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa
     2581 tgatggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg
     2641 gagttaccga gatgtttgat gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc
     2701 agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag
     2761 tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca
     2821 ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg aactttaacc
     2881 acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg
     2941 cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc
     3001 tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt agcattgtcg
     3061 ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag
     3121 aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg
     3181 gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta
     3241 acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa
     3301 ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc
     3361 ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac
     3421 cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc
     3481 tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag
     3541 cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg
     3601 caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc
     3661 gtccggattc tccaccgacc cactggaaga acagcacagc gtagtgcctg gtttgttgca
     3721 taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgcta
     3781 ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta actggcagac
     3841 tgcacttgag tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga
     3901 tccgctgatg gcgaaagagc acgagctgga ctggttgctc acacaactgg aagccatccc
     3961 gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg cacgtatcac
     4021 cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat
     4081 caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt
     4141 cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacg
//
LOCUS       DAJTRM010000373         2484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.226, whole genome shotgun sequence.
ACCESSION   DAJTRM010000373 DAJTRM010000000
VERSION     DAJTRM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.226"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            128..1333
                     /gene="tet(B)"
                     /locus_tag="OO322_004419"
     CDS             128..1333
                     /gene="tet(B)"
                     /locus_tag="OO322_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5908998.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1446..2039)
                     /gene="tetC"
                     /locus_tag="OO322_004420"
     CDS             complement(1446..2039)
                     /gene="tetC"
                     /locus_tag="OO322_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5908999.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2127..>2484
                     /locus_tag="OO322_004421"
     CDS             2127..>2484
                     /locus_tag="OO322_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5909000.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIF"
BASE COUNT          671 a          437 c          510 g          866 t
ORIGIN      
        1 gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat
       61 ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa
      121 aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg
      181 gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga
      241 tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc
      301 tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt
      361 aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta
      421 tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat
      481 tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt
      541 tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca
      601 tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg
      661 gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca
      721 atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt
      781 ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg
      841 ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc
      901 agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt
      961 actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg
     1021 ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt
     1081 acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt
     1141 ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa
     1201 tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat
     1261 tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga
     1321 gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc
     1381 ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat
     1441 ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaattggcct
     1501 tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat aaaccagcca
     1561 ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc ttggatcaat
     1621 ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt ttcaagttca
     1681 ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt attatcaata
     1741 atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt tgttaaatca
     1801 gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca ggcactgagt
     1861 aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa cgcttttccc
     1921 gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat tcgtgctgag
     1981 ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt attttccatg
     2041 atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg tggtcgtgac
     2101 aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc tatcaaaata
     2161 aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg gttatcaaag
     2221 atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat gatgtggcga
     2281 ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta acaggggtca
     2341 cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga
     2401 cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgactcccaa caatctttta
     2461 cacgtcgatt taagtacatt ttta
//
LOCUS       DAJTRM010000374         2120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.234, whole genome shotgun sequence.
ACCESSION   DAJTRM010000374 DAJTRM010000000
VERSION     DAJTRM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.234"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            complement(1..664)
                     /gene="intI2"
                     /locus_tag="OO322_004422"
                     /pseudo
     CDS             complement(1..664)
                     /gene="intI2"
                     /locus_tag="OO322_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            996..1469
                     /locus_tag="OO322_004423"
     CDS             996..1469
                     /locus_tag="OO322_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5909001.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1564..2088
                     /gene="sat2"
                     /locus_tag="OO322_004424"
     CDS             1564..2088
                     /gene="sat2"
                     /locus_tag="OO322_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5909002.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          622 a          449 c          472 g          577 t
ORIGIN      
        1 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
       61 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
      121 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
      181 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
      241 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
      301 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
      361 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
      421 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
      481 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
      541 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
      601 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
      661 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
      721 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
      781 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
      841 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
      901 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
      961 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     1021 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     1081 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     1141 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     1201 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     1261 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     1321 aagtagatac actacatata tctacaatag acatcgagcc ggaaggtgat gtttactttc
     1381 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     1441 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     1501 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     1561 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     1621 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     1681 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     1741 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     1801 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     1861 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     1921 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     1981 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     2041 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     2101 gccgacaccg cttcgcggcg
//
LOCUS       DAJTRM010000375          988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.280, whole genome shotgun sequence.
ACCESSION   DAJTRM010000375 DAJTRM010000000
VERSION     DAJTRM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.280"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            137..928
                     /gene="aadA1"
                     /locus_tag="OO322_004425"
     CDS             137..928
                     /gene="aadA1"
                     /locus_tag="OO322_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5909003.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          251 a          251 c          261 g          225 t
ORIGIN      
        1 attctaaaca cactaaattt ataaaaaatc taatggcaaa atcgcccaac ccttcaatca
       61 agtcgggacg gccaaaagca agcttttggc tcccctcgct ggcgctcggc gccccttatt
      121 tcaaacgtta aacatcatga gggaagtggt gatcgccgaa gtatcgactc aactatcaga
      181 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
      241 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
      301 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
      361 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
      421 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
      481 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
      541 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
      601 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
      661 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
      721 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
      781 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
      841 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg
      901 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt
      961 cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAJTRM010000376          985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.281, whole genome shotgun sequence.
ACCESSION   DAJTRM010000376 DAJTRM010000000
VERSION     DAJTRM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.281"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            137..925
                     /gene="aadA1"
                     /locus_tag="OO322_004426"
     CDS             137..925
                     /gene="aadA1"
                     /locus_tag="OO322_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5909004.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          248 a          251 c          261 g          225 t
ORIGIN      
        1 attctaaaca cactaaattt ataaaaaatc taatggcaaa atcgcccaac ccttcaatca
       61 agtcgggacg gccaaaagca agcttttggc tcccctcgct ggcgctcggc gccccttatt
      121 tcaaacgtta aacatcatga gggaagtggt gatcgccgaa gtatcgactc aactatcaga
      181 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
      241 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
      301 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
      361 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
      421 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
      481 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
      541 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
      601 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
      661 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
      721 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
      781 cctgccggcc cagtatcagc ccgtcatact tgaagctagg caggcttatc ttggacaaga
      841 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga
      901 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc
      961 ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAJTRM010000377          917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.285, whole genome shotgun sequence.
ACCESSION   DAJTRM010000377 DAJTRM010000000
VERSION     DAJTRM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.285"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            66..857
                     /gene="aadA1"
                     /locus_tag="OO322_004427"
     CDS             66..857
                     /gene="aadA1"
                     /locus_tag="OO322_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5909005.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          226 a          232 c          253 g          206 t
ORIGIN      
        1 acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt caggagttaa
       61 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      121 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      181 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
      241 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      301 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      361 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
      421 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
      481 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
      541 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
      601 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
      661 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
      721 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
      781 tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca
      841 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
      901 ttaactcaag cgttaga
//
LOCUS       DAJTRM010000378          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600702
            SAMEA8900410-rid15818693.guided.287, whole genome shotgun sequence.
ACCESSION   DAJTRM010000378 DAJTRM010000000
VERSION     DAJTRM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900410
            Sequence Read Archive: ERR6004801
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477715.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477715.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:49:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,355
            Genes (coding)                    :: 3,968
            CDSs (with protein)               :: 3,968
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 61
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 387
            CDSs (without protein)            :: 387
            Pseudo Genes (ambiguous residues) :: 0 of 387
            Pseudo Genes (frameshifted)       :: 185 of 387
            Pseudo Genes (incomplete)         :: 101 of 387
            Pseudo Genes (internal stop)      :: 145 of 387
            Pseudo Genes (multiple problems)  :: 43 of 387
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900410-rid15818693.guided.287"
                     /serovar="S. flexneri 2b"
                     /isolate="600702"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-06-20"
     gene            66..854
                     /gene="aadA1"
                     /locus_tag="OO322_004428"
     CDS             66..854
                     /gene="aadA1"
                     /locus_tag="OO322_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5909006.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          223 a          232 c          253 g          206 t
ORIGIN      
        1 acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt caggagttaa
       61 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      121 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      181 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
      241 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      301 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      361 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
      421 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
      481 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
      541 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
      601 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
      661 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
      721 agtatcagcc cgtcatactt gaagctaggc aggcttatct tggacaagaa gatcgcttgg
      781 cctcgcgcgc agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg
      841 tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta
      901 actcaagcgt taga
//
